LOCUS       DAVLVP010000001        67741 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.001, whole genome shotgun sequence.
ACCESSION   DAVLVP010000001 DAVLVP010000000
VERSION     DAVLVP010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 67741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67741
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.001"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            231..527
                     /locus_tag="VYN69_000001"
     CDS             231..527
                     /locus_tag="VYN69_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000380708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEV1532364.1"
                     /translation="MDTPDENGYVADNYRITYLEAHIKAMRDAIYQDGVDLLGYTTWS
                     CIDPVSAGTGEMNKRYGFIYVDRDNVGNGALKRSKKKSFYWYKDVIDSNGASIG"
     gene            648..1592
                     /gene="manA"
                     /locus_tag="VYN69_000002"
     CDS             648..1592
                     /gene="manA"
                     /locus_tag="VYN69_000002"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEV1532365.1"
                     /translation="MSEPLFLQSVMQEKIWGGAKLRDEFGYDIPSEKIGEYWAISAHP
                     NGVSKVANGRYEGTDLATLYAEHRELFGNRPEPVFPLLTKILDANDWLSVQVHPDDAY
                     GLEHEGELGKTECWYIIAADEGSEIIYGHNAKSKEELRQQIEDKNWDALLTKVPVKAG
                     DFFYVPSGTMHAIGAGILILETQQSSDTTYRVYDFDRKDDKGNLRELHLEKSIDVLNI
                     GEPANSRPVTIKADDLRSTLLVSNDFFAVYKWEITGKVNFEKTADYSLLSVLAGQGQL
                     TVDGKNYPIQKGRHFILPSDVESWTLEGQDLELIVSHP"
     gene            complement(1632..2957)
                     /locus_tag="VYN69_000003"
     CDS             complement(1632..2957)
                     /locus_tag="VYN69_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HEV1532366.1"
                     /translation="MSEKSQWGSKLGFILASAGSVIGLGSVWKFPYMTAANGGGGFLL
                     IFLISTILIDFPLLLAEFALGRSAGVSAIKTFGKLGKNNKYNFIGWIGAFALFILLSF
                     YSVIGGWILVYLGIEFGKLFQLGGTGDYAQLFTSIISNPAIALGAQATFILLNIFIVS
                     RGVQKGIERASKVMMPLLFIIFVVIIERSLSLPNAMEGVLYFLKPDFSKLTSAGLLYA
                     LRQSFFALSLGVTAMLTYASYLDKKTNLVQSRISIVAMNISVSIMAGLAIFQARSPFN
                     IQSEGGPSLVFIVLPQLFDKMPFGTIFYVLFLFATVTSSVVMLEINVDNITNQDNSKR
                     AKWSVILGILTFVFGIPSALSYGVMADVHIFGKAFFDAMDFLVSNLLMPFGALFLSLF
                     TGYIFKKALAMEELHLDERAWKQGLFQVWLFLLRFVIPIIIVVFIAQFM"
     gene            3219..3311
                     /locus_tag="VYN69_000004"
     CDS             3219..3311
                     /locus_tag="VYN69_000004"
                     /inference="COORDINATES: protein motif:HMM:NF024803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1532367.1"
                     /translation="MHHYDKIGLLVPLKSENGYRTYSQEDLERL"
     gene            3315..3908
                     /locus_tag="VYN69_000005"
     CDS             3315..3908
                     /locus_tag="VYN69_000005"
                     /inference="COORDINATES: protein motif:HMM:NF020839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TipAS antibiotic-recognition domain-containing
                     protein"
                     /protein_id="HEV1532368.1"
                     /translation="MLYYKYLGFSLEKIAELLKEERTDLLPHLTRQLDYLTRERQHLD
                     TLISTLQKTIQEQKGERKMTIEEKFTGFSYQDNQKYHQEAVEKYGQEVMGQALERQKG
                     HEDEATAAFNQVFQTLAQNLQVGLPATATENQEQAAKLLQAIRTYGFDCSIEVFGHIG
                     KGYVYNPEFKENIDKFGSGTAQYTSDAIAAYVQTNAE"
     gene            4015..5305
                     /locus_tag="VYN69_000006"
                     /pseudo
     CDS             4015..5305
                     /locus_tag="VYN69_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002937360.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            complement(5456..5911)
                     /locus_tag="VYN69_000007"
     CDS             complement(5456..5911)
                     /locus_tag="VYN69_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1532369.1"
                     /translation="MNRREAVEFVNMCMIKNGDKVLVQDRVSPDWSGITFPGGHVERG
                     ESFVDAVIREVKEETGLIISKPQLCGIKNWYDDKDYRYVVLFYKTEHFTGELQSSDEG
                     KVWWEDFENLSHLKLATDDMSDMLRVFLEEDLSEFFYYKNGDDWLYDLK"
     gene            complement(5931..7106)
                     /locus_tag="VYN69_000008"
     CDS             complement(5931..7106)
                     /locus_tag="VYN69_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1532370.1"
                     /translation="MKHFDTIVIGGGPAGMMATISSSFYGQKTLLIEKNRKLGKKLAG
                     TGGGRCNVTNNGSLDNLLAGIPGNGRFLYSVFSQFDNHDIINFFTENGVKLKVEDHGR
                     VFPASDKSRTIIEALEKKITELGGQVATQIEIVSVKKVDDQFVLKSADQTFTCEKLIV
                     TTGGKSYPSTGSTGFGHEIARHFKHTITDLEAAESPLLTDFPHKALQGISLDDVTLSY
                     GKHVITHDLLFTHFGLSGPAALRMSSFVKGGEVLSLDVLPQLSEKDLVTFLEENREKS
                     LKNALKTLLPERLAEFFVQGYPEKVKQLTEKEREQLVQSIKELKIPVTGKMSLAKSFV
                     TKGGVSLKEINPKTLESKLVPGLHFAGEVMDINAHTGGFNITSALCTGWVAGSLHYD"
     gene            complement(7165..8010)
                     /locus_tag="VYN69_000009"
     CDS             complement(7165..8010)
                     /locus_tag="VYN69_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1532371.1"
                     /translation="MTWKIIADSGCDYRQLPTPAINTTFVSVPLTIQVADQVFVDDAS
                     LDIDQMMETMYATAEASKSACPSPDDYLRAFEGAKNIFLVTITGTLSGSHNSAQLAKN
                     IYLEDHPDTKIHVIDSLSAGGEVDLLVEKLNDLIDQGLSFEEVVEAITAYQEKTKLLF
                     VLAKVDNLVKNGRLSKLIGTVVGLLNIRMVGKASETGTLELLQKARGSKKSVQAAYDE
                     LVKAGYAGGRIVMAQRNNEKCCQQLSERIRETFPQADIKILPTSGLCSFYAEDGGLLM
                     GYEID"
     gene            8135..8692
                     /locus_tag="VYN69_000010"
     CDS             8135..8692
                     /locus_tag="VYN69_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1532372.1"
                     /translation="MSERRISEKSLENLRKSNQESNLLTREAIETALLQLLEKKELTK
                     ISISELVKRAGVSRAAFYRNYDSKEEILESVFKRTVHNIMEQLHHYDLKTDLYLVWVH
                     LFREARKEARVIQLALDYHLEKIFVQAMQEFLEKYHGKSKGVSSYLHSFWSSAIVSVL
                     LKWIKDGMKVPAEKIADLGLPFFKK"
     gene            8711..9178
                     /locus_tag="VYN69_000011"
     CDS             8711..9178
                     /locus_tag="VYN69_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HEV1532373.1"
                     /translation="MTEKRLAWDEYFAAQALLIANRSTCKRAKVGAILVKDNKVISTG
                     YNGSVSGTEHCIDHECLVIEGHCVRTLHAEVNAILQGAERGVPKGFTAYVTHFPCLNC
                     TKQLLQVGCKRVVYINQYRMDDYAQYLYQEKGTELTHLPLETVQTALKEADLM"
     gene            9267..9896
                     /gene="upp"
                     /locus_tag="VYN69_000012"
     CDS             9267..9896
                     /gene="upp"
                     /locus_tag="VYN69_000012"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002883819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEV1532374.1"
                     /translation="MGKIEVINHPLIQHKLSILRRTDTSTKAFRELVDEIAMLMGYEV
                     LRDLPLEDVEIETPITKTVQKQLAGKKLAIVPILRAGIGMVDGLLSLVPAAKVGHIGM
                     YRDEETLQPVEYLVKLPEDIDQRQIFVVDPMLATGGSAILAVDSLKKRGASNIKFVCL
                     VSAPEGVKALQEAHPDVEIFTAALDERLNEHGYIVPGLGDAGDRLFGTK"
     gene            10070..10660
                     /gene="clpP"
                     /locus_tag="VYN69_000013"
     CDS             10070..10660
                     /gene="clpP"
                     /locus_tag="VYN69_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit
                     ClpP"
                     /protein_id="HEV1532375.1"
                     /translation="MIPVVIEQTSRGERSYDIYSRLLKDRIIMLTGPVEDNMANSVIA
                     QLLFLDAQDSTKDIYLYVNTPGGSVSAGLAIVDTMNFIKADVQTIVMGMAASMGTVIA
                     SSGAKGKRFMLPNAEYMIHQPMGGTGGGTQQTDMAIAAEHLLKTRNTLEKILAENSGQ
                     SMEKVHADAERDNWMSSQETLEYGFIDEIMANNSLN"
     gene            10739..10987
                     /locus_tag="VYN69_000014"
     CDS             10739..10987
                     /locus_tag="VYN69_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEV1532376.1"
                     /translation="MFEKVNRSGLIIYLYYNRDAKKLQDYGDITYHSKKHRYLQLYVP
                     TQEVEQLVGRLSKEKFIKKVRVCHIQDLETPFVGNLYR"
     gene            11088..12248
                     /locus_tag="VYN69_000015"
     CDS             11088..12248
                     /locus_tag="VYN69_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1532377.1"
                     /translation="MKKKFALSFVALASVALLAACGEVKSGAVNTAGNSVEEKTIKIG
                     FNFEESGSLAAYGTAEQKGAQLAVDEINAAGGIDGKQIEVVDKDNKSETAEAASVTTN
                     LVTQSKVSAVVGPATSGATAAAVANATKAGVPLISPSATQDGLTKGQDYLFIGTFQDS
                     FQGKIISNYVSEKLNAKKVVLYTDNASDYAKGIAKSFRESYKGEIVADETFVAGDTDF
                     QAALTKMKGKDFDAIVVPGYYNEAGKIVNQARGMGIDKPIVGGDGFNGEEFVQQATAE
                     KASNIYFISGFSTTVEVSAKAKAFLDAYRAKYNEEPSTFAALAYDSVHLVANAAKGAK
                     NSGEIKNNLAKTKDFEGVTGQTSFDADHNTVKTAYMMTMNNGKVEAAEVVKP"
     gene            12516..13385
                     /locus_tag="VYN69_000016"
     CDS             12516..13385
                     /locus_tag="VYN69_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1532378.1"
                     /translation="MLQQLVNGLILGSVYALLALGYTMVYGIIKLINFAHGDIYMMGA
                     FIGYFLINSFQMNFFVALIVAMLVTAILGVVIEFLAYRPLRHSTRIAVLITAIGVSFL
                     LEYGMVYLVGANTRAFPQAIQTVRYDLGPISLTNVQLMILAISLILMILLQVIVQKTK
                     MGKAMRAVSVDSDAAQLMGINVNRTISFTFALGSALAGAAGVLIALYYNSLEPLMGVT
                     PGLKSFVAAVLGGIGIIPGAALGGFVIGLLETFATAFGMSDFRDAIVYGILLLILIVR
                     PAGILGKNVKEKV"
     gene            13389..14345
                     /locus_tag="VYN69_000017"
     CDS             13389..14345
                     /locus_tag="VYN69_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HEV1532379.1"
                     /translation="MKENLKVNILWLLLLLAGYSLISVLVSVGVLNLFYVQILQQIGI
                     NIILAVGLNLIVGFSGQFSLGHAGFMAIGAYAAAIIGSKSPTYGAFFGAMLVGALLSG
                     AVALLVGIPTLRLKGDYLAVATLGVSEIIRIFIINGGSLTNGAAGILGIPNFTTWQMV
                     YFFVVITTIATLNFLRSPIGRSTLSVREDEIAAESVGVNTTKIKIIAFVFGAITASIA
                     GSLQAGFIGSVVPKDYTFINSINVLIIVVFGGLGSITGAIVSAIVLGILNMLLQDVAS
                     VRMIIYALALVLVMIFRPGGLLGTWELSLSRFFKKSKKEEQN"
     gene            14345..15109
                     /locus_tag="VYN69_000018"
     CDS             14345..15109
                     /locus_tag="VYN69_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1532380.1"
                     /translation="MALLEVKQLTKHFGGLTAVGDVTLELNEGELVGLIGPNGAGKTT
                     LFNLLTGVYEPSEGTVTLDGHLLNGKSPYKIASLGLGRTFQNIRLFKDLTVLDNVLIA
                     FGNHHKQHVFTSFLRLPAFYKSEKELKAKALELLKIFDLDGDAETLAKNLSYGQQRRL
                     EIVRALATEPKILFLDEPAAGMNPQETAELTELIRRIKDEFKITIMLIEHDMNLVMEV
                     TERIYVLEYGRLIAQGTPDEIKTNKRVIEAYLGGEA"
     gene            15109..15819
                     /locus_tag="VYN69_000019"
     CDS             15109..15819
                     /locus_tag="VYN69_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1532381.1"
                     /translation="MSMLKVENLSVHYGMIQAVRDVSFEVNEGEVVSLIGANGAGKTT
                     ILRTLSGLVRPSSGKIEFLGQEIQKMPAQKIVAGGLSQVPEGRHVFPGLTVMENLEMG
                     AFLKKNREENQANLKKVFSRFPRLEERKNQDAATLSGGEQQMLAMGRALMSTPKLLLL
                     DEPSMGLAPIFIQEIFDIIQDIQKQGTTVLLIEQNANKALAISDRGYVLETGKIVLSG
                     SGKELASSEEVRKAYLGG"
     gene            16127..16783
                     /locus_tag="VYN69_000020"
     CDS             16127..16783
                     /locus_tag="VYN69_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1532382.1"
                     /translation="MAVKDFMTRKVVYISPDTTVSHAADLMREQGLHRLPVIENDQLV
                     GLVTEGTIAQASPSKATSLSIYEMNYLLNKTKVKDVMIRDVVTVSGYASLEDATYLML
                     KNKIGILPVVDNHQVYGVITDRDVFQAFLEIAGYGEEGIRVRFVTEDEVGVLGKIVSL
                     IVEENLNISHTVNIPRKDGKVIIEVQIDESIDLPALKEKFEANGIQVEEIARTSAKVL
                     "
     gene            16891..17986
                     /gene="prfB"
                     /locus_tag="VYN69_000021"
     CDS             join(16891..16962,16964..17986)
                     /gene="prfB"
                     /locus_tag="VYN69_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511312.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEV1532383.1"
                     /translation="MDISVIRQKIDANREKLASFRGSLDLEGLEEEIAILENKMTEPD
                     FWNDNIAAQKTSQELNELKNTYNTFHKMEELQDEVEILLDFLAEDESVHDELVAQLAE
                     LDKIMTSYEMTLLLSEPYDHNNAILEIHPGSGGTEAQDWGDMLLRMYTRYGNAKGFKV
                     EVLDYQAGDEAGIKSVTLSFEGPNAYGLLKSEMGVHRLVRISPFDSAKRRHTSFTSVE
                     VMPELDDTIEVEIREDDIKMDTFRSGGAGGQNVNKVSTGVRLTYIPTGIVVQSTVDRT
                     QYGNRDRAMKMLQAKLYQMEQEKKAAEVDSLKGEKKEITWGSQIRSYVFTPYTMVKDH
                     RTSFEVAQVDKVMDGDLDGFIDAYLKWRIS"
     gene            18004..18696
                     /gene="ftsE"
                     /locus_tag="VYN69_000022"
     CDS             18004..18696
                     /gene="ftsE"
                     /locus_tag="VYN69_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEV1532384.1"
                     /translation="MSIIEMRDVVKKYDNGTTALRGVSVSVQPGEFAYIVGPSGAGKS
                     TFIRSLYREVKIDKGSLSVAGFNLVKIKKKDVPLLRRSVGVVFQDYKLLPKKTVYENI
                     AYAMEVIGENRRNIKRRVMEVLDLVGLKHKVRSFPNELSGGEQQRIAIARAIVNNPKV
                     LIADEPTGNLDPDNSWEIMNLLERINLQGTTILMATHNSQIVNTLRHRVIAIENGRVV
                     RDESKGEYGYDD"
     gene            18689..19615
                     /locus_tag="VYN69_000023"
     CDS             18689..19615
                     /locus_tag="VYN69_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1532385.1"
                     /translation="MISRFFRHLFEALKSLKRNGWMTVAAVSSVMITLTLVAIFASVI
                     FNTAKLATDIENNVRVVVYIRKDVEDNSQTIEKEGQTVTNNDYHKVYDSLKNMSTVKS
                     VTFSSKEEQYEKLTEIMGDNWKIFEGDANPLYDAYIVEANAPNDVKTIAEDAKKIEGV
                     SEVQDGGANTERLFKLASFIRVWGLGIAALLIFIAVFLISNTIRITIISRSREIQIMR
                     LVGAKNSYIRGPFLLEGAFIGLLGAIAPSVLVFIVYQIVYQSVNKSLVGQNLSMISPD
                     LFSPLMIALLFVIGVFIGSLGSGISMRRFLKI"
     gene            19901..22081
                     /locus_tag="VYN69_000024"
     CDS             19901..22081
                     /locus_tag="VYN69_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000974069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1532386.1"
                     /translation="MMKDTFKNVLSFEFWQKFGKALMVVIAVMPAAGLMISIGKSIVM
                     INPTFAPLVITGGILEQIGWGVIGNLHILFALAIGGSWAKERAGGAFAAGLAFILINR
                     ITGTIFGVSGDMLKNPDAMVTTFFGGSIKVADYFISVLEAPALNMGVFVGIISGFVGA
                     TAYNKYYNFRKLPDALSFFNGKRFVPFVVILRSAIAAILLAAFWPVVQTGINNFGIWI
                     ANSQETAPILAPFLYGTLERLLLPFGLHHMLTIPMNYTALGGTYDILTGAAKGTQVFG
                     QDPLWLAWVTDLVNLKGTDASQYQHLLDTVHPARFKVGQMIGSFGILMGVIVAIYRNV
                     DADKKHKYKGMMIATALATFLTGVTEPIEYMFMFIATPMYLVYSLVQGAAFAMADVVN
                     LRMHSFGSIEFLTRTPIAISAGIGMDIVNFVWVTVLFAVIMYFIANFMIQKFNYATPG
                     RNGNYETAEGSEETSSEVKVAAGSQAVNIINLLGGRVNIVDVDACMTRLRVTVKDADK
                     VGNAEQWKAEGAMGLVMKGQGVQAIYGPKADILKSDIQDILDSGEIIPETLPSQMTEV
                     QQNTVHFKDLTEEVYSVADGQVVALEQVKDPVFAQKMMGDGFAVEPANGNIVSPVSGT
                     VSSIFPTKHAFGIVTEAGLEVLVHIGLDTVSLEGKPFTVHVAEGQKVAAGDLLVTADL
                     NAIRAAGRETSTVVVFTNGDAIKSVKLEKTGSLAAKTAVAKVEL"
     gene            22133..22948
                     /locus_tag="VYN69_000025"
     CDS             22133..22948
                     /locus_tag="VYN69_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1532387.1"
                     /translation="MKFLTLNTHSWMEKEAEEKFQILLEDILEKDYDLICFQEINQEM
                     TSSEVEVNDLYQALPAAEPIHQDHYVRLLVEKLSEQGKNYYWTWAYNHIGYDRYHEGV
                     AILSKTPIEAREILVSDVDDPTDYHTRRVALAETVVDGKELAVASVHLSWWDKGFQEE
                     WARFEVVLKKLNKPLLLAGDFNNPAGQEGYQAILASPLGLQDAFEVAQEKSGSYTVPP
                     EIDGWKGNTEPLRIDYVFTTKELAVENLHVVFDGNKSPQVSDHYGLNAMLNWK"
     gene            23088..24431
                     /locus_tag="VYN69_000026"
     CDS             23088..24431
                     /locus_tag="VYN69_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1532388.1"
                     /translation="MSFTKFQFKNYIREALEELKFTTPTEVQDKLIPIVLAGRDLVGE
                     SKTGSGKTHTFLLPIFQQLDEASDSVQAVITAPSRELATQIYQVARQISAHSDVEVRV
                     VNYVGGTDKARQIEKLESNQPHIVIGTPGRIYDLVKSGDLAIHKAKTFVVDEADMTLD
                     MGFLETVDKIAGSLPKDLQFMVFSATIPQKLQPFLKKYLSNPVMEKIKTKTVISDTID
                     NWLISTKGHDKNAQIYQLTQLMQPYLAMIFVNTKTRADELHSYLTAQGLKVAKIHGDI
                     APRERKRIMNQVQNLDFEYIVATDLAARGIDIEGVSHVINDAIPQDLSFFVHRVGRTG
                     RNGLPGTAITLYQPSDDSDIRELEKLGIKFSPKMVKDGEFQDTYDRDRRANREKKQDK
                     LDIEMIGLVKKKKKKVKPGYKKKIQWAVDEKRRKTKRAENRARGRAERKAKRQTF"
     gene            24646..25836
                     /locus_tag="VYN69_000027"
     CDS             24646..25836
                     /locus_tag="VYN69_000027"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000003937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEV1532389.1"
                     /translation="MSERKLFTSESVSEGHPDKIADQISDAILDAILAKDPEAHVAAE
                     TAVYTGSVHVFGEISTNAYVDINRVVRDTIAEIGYTNTEYGFSAETVGVHPSLVEQSP
                     DIAQGVNEALEVRGNADQDPLDLIGAGDQGLMFGFAVDETEELMPLPIALSHKLVRRL
                     AELRKSGEISYLRPDAKSQVTVEYDENDRPVRVDTVVISTQHDPEATNEQIHQDVIDK
                     VIKEVIPSSYLDDKTKFFINPTGRFVIGGPQGDSGLTGRKIIVDTYGGYSRHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLAKKAEVQLAYAIGVAQPVSVRIDTFGTGTVA
                     ESQLEKAARQIFDLRPAGIIQMLDLKRPIYRQTSAYGHMGRTDIDLPWERLDKVDALK
                     EAVK"
     gene            26389..27324
                     /locus_tag="VYN69_000028"
     CDS             26389..27324
                     /locus_tag="VYN69_000028"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEV1532390.1"
                     /translation="MVSTKTQIAGFEFDNCLMNAAGVACMTIEELEEVKNSAAGTFVT
                     KTATLDFRQGNPEPRYQDVPLGSINSMGLPNNGLDYYLDYLLDLQEKESNRTFFLSLV
                     GMSPEETHTILKKVQESDFRGLTELNLSCPNVPGKPQIAYDFETTDRILAEVFAYFTK
                     PLGIKLPPYFDIVHFDQAAAIFNKYPLKFVNCVNSIGNGLYIEDESVVIRPKNGFGGI
                     GGEYIKPTALANVHAFYQRLNPQIQIIGTGGVLTGRDAFEHILCGASMVQVGTTLHKE
                     GVSAFDRITNELKAIMVEKGYESLEDFRGKLRYID"
     gene            27358..28395
                     /locus_tag="VYN69_000029"
     CDS             27358..28395
                     /locus_tag="VYN69_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001845438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1532391.1"
                     /translation="MLAIEESQKLTLSNLPSLSLFTGTDQGQFEVMKSQVLKQIGYDS
                     ADLNFAYFDMKEVVYKDVELELVSLPFFADEKIVILDYFMDITTAKKRFLTDDELKSF
                     EEYLDNPSPTTKLIIFAEGKLDSKRRLVKLLKRDAKVFDAVEVKEQELRQYFQKWSQK
                     QGLQFTNHSFENLLIKSGFQFSEIQKNLLFLQSYKVDSVIEEEDIVNAIPKTLQDNIF
                     DLTQFILTKKMDQARDLVRDLTLQGEDEIKLIAVMLGQFRTFTQVKILAESGQTESQI
                     ASSLGSYLGRNPNPYQIKFALRDSRGLSLSFLKQAISYLIETDYQIKTGLYEKGFLFE
                     KALLQIASQVN"
     gene            28568..29173
                     /locus_tag="VYN69_000030"
     CDS             28568..29173
                     /locus_tag="VYN69_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEV1532392.1"
                     /translation="MAIILPELPYAYDALEPYIDAETMHLHHDKHHQTYVNNANAALE
                     KHPEIGEDLEALLADVESIPADIRQALINNGGGHLNHALFWELMTPEKTAPSAELAAA
                     IDATFGSFEEFQAAFTAAATTRFGSGWAWLVVNKEGKLEVTSTANQDTPISEGKKPIL
                     GLDVWEHAYYVKYRNVRPDYIKAFFSVINWNKVDELYAAAK"
     gene            29331..29861
                     /locus_tag="VYN69_000031"
     CDS             29331..29861
                     /locus_tag="VYN69_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEV1532393.1"
                     /translation="MRKEIAPELYNYNKFPGPEFHLHGDKVETEGIAFSLVENIKDAF
                     DVTTFNQRFSEVLTKFDYIVGDWSNEQLRLRGFYKDDRTEEKLEKISRLQDYLLEYCS
                     YGCAYFVLENEAPKRASFDKKMRKTEEETPSRKGKKPTQTKRKSNADKKNRRRQKDQH
                     SQKEDKEQRHFVIRQK"
     gene            29882..30967
                     /gene="rlmN"
                     /locus_tag="VYN69_000032"
     CDS             29882..30967
                     /gene="rlmN"
                     /locus_tag="VYN69_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEV1532394.1"
                     /translation="MKPSIHSLVHQTMQEWVLEQGEKKFRADQIWEWLYRKRVQSFEE
                     MTNLSKDLIAKLNDQFVVNPLKQRIVQESADGTVKYLFELPDGMLIETVLMRQHYGLS
                     VCVTTQVGCNIGCTFCASGLIKKQRDLNNGEIVAQIMLVQKYFDERGQDERVSHIVVM
                     GIGEPFDNYNNVLNFFRTINDDKGMAIGARHITVSISGLAHKIRDFADEGVQVNLAVS
                     LHAPNNELRSSIMKINRAFPIEKLFAAIEYYIETTNRRVTFEYIMLNEVNDGVEQALE
                     LAELLKNIKKLSYVNLIPYNPVSEHDQYSRSPKERVLAFYDTLKKKGVNCVVRQEHGT
                     DIDAACGQLRSNTMKRDRQKAVAAVNP"
     gene            30967..31449
                     /locus_tag="VYN69_000033"
     CDS             30967..31449
                     /locus_tag="VYN69_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1532395.1"
                     /translation="MMKKTYNHILVWGVIFYSICIVCFCFTPQEQSTVGVGTPDIQHL
                     GRLVFLLTPFNSLWKLGEVSDIGQLCWIFLQNILNVFLFFPLIFQLLYLFPNLRKTKK
                     VLLFSFLVSLGIECTQLILDFFFDFNRVFEIDDLWTNTLGGYLAWLLYKRLHKNKIRN
                     "
     gene            31451..32992
                     /locus_tag="VYN69_000034"
     CDS             31451..32992
                     /locus_tag="VYN69_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002918612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1532396.1"
                     /translation="MSILEVKNLSHGFGDRAIFEDVSFRLLKGEHIGLVGANGEGKST
                     FMSIVTGKMLPDEGKVEWSKYVTAGYLDQHSVLAERQSVRDVLRTAFDELFKAEARIN
                     DLYMKMAEDGADVDALMEEVGELQDRLESRDFYTLDAKIDEVARALGVMDFGMDTDVT
                     SLSGGQRTKVLLAKLLLEKPDILLLDEPTNYLDAEHIDWLKRYLQNYENAFVLISHDI
                     PFLNDVINIVYHVENQQLTRYSGDYYQFQEVYAMKKSQLEAAYERQQKEIADLKDFVA
                     RNKARVATRNMAMSRQKKLDKMDIIELQSEKPKPSFDFKPARTPGRFIFQAKNLQIGY
                     DRPLTKPLNLTFERNQKVAIIGANGIGKTTLLKSLLGIISPIAGEVERGDYLELGYFE
                     QEVEGGNRQTPLEAVWNAFPALNQAEVRAALARCGLTTKHIESQIQVLSGGEQAKVRF
                     CLLMNRENNVLVLDEPTNHLDVDAKDELKRALKEYRGSILMVCHEPDFYEGWIDQIWD
                     FNNLT"
     gene            complement(33048..33851)
                     /locus_tag="VYN69_000035"
     CDS             complement(33048..33851)
                     /locus_tag="VYN69_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEV1532397.1"
                     /translation="MKKLATLLLLSTVALAGCSSVQRSLRGDDYVDSSLAAEESSKVA
                     AQSAKELNDALTNENANFPQLSKEVAEDEAEVILHTSQGDIRIKLFPKLAPLAVENFL
                     THAKEGYYNGITFHRVIDGFMVQTGDPKGDGTGGQSIWHDKDKTKDKGTGFKNEITPY
                     LYNIRGALAMANTGQPNTNGSQFFINQNSTDTSSKLPTSKYPQKIIEAYKEGGNPSLD
                     GKHPVFGQVIDGMDVVDKIAKAEKDEKDKPTTAITIDSIEVVKDYDFKS"
     gene            complement(33935..34147)
                     /locus_tag="VYN69_000036"
     CDS             complement(33935..34147)
                     /locus_tag="VYN69_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532398.1"
                     /translation="MSFYGLFYNGIAITPNTYLSAWFVNFIAALPLNFLIVEPIARFI
                     LSSFQKPFTGEEVEDFQDDDEIPTII"
     gene            complement(34626..34835)
                     /locus_tag="VYN69_000037"
     CDS             complement(34626..34835)
                     /locus_tag="VYN69_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000953868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532399.1"
                     /translation="MLSVNTILEKFYKEHQVKPFISPERELDTWLLSPKPVPKRNMDL
                     LVDDSLAGDIILLWRIQFGTFTTET"
     gene            35005..35277
                     /gene="rpsP"
                     /locus_tag="VYN69_000038"
     CDS             35005..35277
                     /gene="rpsP"
                     /locus_tag="VYN69_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002884675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEV1532400.1"
                     /translation="MAVKIRLTRMGSKKKPFYRINVADSRSPRDGRFIETVGTYNPLV
                     AENQVTLKEDRVLAWLANGAQPSDTVRNILSKEGVLKKFHDSKFSK"
     gene            35297..35536
                     /locus_tag="VYN69_000039"
     CDS             35297..35536
                     /locus_tag="VYN69_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEV1532401.1"
                     /translation="MDTIENLIIAIVKPLISQPDALTIKIEDTPEFLEYHLNLDQSDV
                     GRVIGRKGRTISAIRTIVYSVPTEYKKVRIVIDEK"
     gene            36113..36901
                     /locus_tag="VYN69_000040"
     CDS             36113..36901
                     /locus_tag="VYN69_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1532402.1"
                     /translation="MKRFEVSTEIGSLSVTYQKQKKVLVCLNGAGLLPSYENFSLILE
                     KPPPTIGYLTIDFPNTGRSPIHDQAGKNLDNLADAVYEVLEELGISEYILCAHSWSGI
                     LACKLLEKPIKRQTLVAIEPTTKKVMFADFSENPYPKMEEQMRLIDECGPELYFKNLT
                     QATFSPETNKKIWELMQEKGLELENQDPEFQISGEITEEDFENLSIESHVPVFIFCQT
                     YREKEYRESEYWTSNTKLILGGNHHYLQWSESEKIAAIIRELSE"
     gene            36932..37450
                     /gene="rimM"
                     /locus_tag="VYN69_000041"
     CDS             36932..37450
                     /gene="rimM"
                     /locus_tag="VYN69_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEV1532403.1"
                     /translation="MNYFNVGKIVNTQGLQGEMRVLSVTDFAEERFKKGAELALFDEK
                     DQFVQTVTIASHRKQKNFDIIKFKDMYHINTIEKYKGYSLKVAEEDLNDLDDGEFYYH
                     EIIGLEVYEGDSLVGTIKEILQPGANDVWVVKRKGKRDLLLPYIPPVVLNVDIPNKRV
                     DVEILEGLDDED"
     gene            37440..38159
                     /gene="trmD"
                     /locus_tag="VYN69_000042"
     CDS             37440..38159
                     /gene="trmD"
                     /locus_tag="VYN69_000042"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEV1532404.1"
                     /translation="MKIDILTLFPEMFSPLEHSIVGKAREKGLLDIQYHNFRENAEKA
                     RHVDDDPYGGGQGMLLRAQPIFDSFDAIEKKNPRVILLDPAGKQFDQAYAEDLAQEEE
                     LIFICGHYEGYDERIKTLVTDEISLGDYVLTGGELAAMTMIDATVRLIPEVIGKESSH
                     QDDSFSSGLLEYPQYTRPYDYRGMVVPDVLMSGHHEKIRQWRLYESLKKTYERRPDLL
                     EHYQLTVEEEKMLAEIKENKE"
     gene            38171..38509
                     /locus_tag="VYN69_000043"
     CDS             38171..38509
                     /locus_tag="VYN69_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532405.1"
                     /translation="MQVIKRNGEIAEFNPDKIYQAILKAAQTVYVLTDDLRQNLAQVT
                     KKVVLDLQEAKVERATISMIQSMVEHRLLGAGYITIAEHYISYRLQRDLERSGYGDHI
                     AVHLHFEQIR"
     gene            complement(38540..38860)
                     /locus_tag="VYN69_000044"
     CDS             complement(38540..38860)
                     /locus_tag="VYN69_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001216095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532406.1"
                     /translation="MRHKFQQVLDKIHDFLNGHDQPDQTETNSLTATIEEAIQKQTAV
                     HLILSETSFTGDIIKYDQQGQQIIVKNFAKNVSRIIRISDIQRLRFVPSTVQTAQKNR
                     FKKE"
     gene            38958..39173
                     /locus_tag="VYN69_000045"
     CDS             38958..39173
                     /locus_tag="VYN69_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000807422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEV1532407.1"
                     /translation="MAEFTFEIEEHLLTLSENEKGWTKEINRVSFNGAPAKFDIRAWS
                     PDHTKMGKGITLSNEEFQTMVDAFKGN"
     gene            complement(39287..39373)
                     /locus_tag="VYN69_000046"
                     /pseudo
     CDS             complement(39287..39373)
                     /locus_tag="VYN69_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(39424..39960)
                     /locus_tag="VYN69_000047"
     CDS             complement(39424..39960)
                     /locus_tag="VYN69_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000709035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEV1532408.1"
                     /translation="MKKAHVYAIPAIGAALIAVLAQISLPIGPVPFTLQNFAIGLIAT
                     VFRPREAVLSAGLYLLLGAIGLPVFAGGGAGFQALVGPTAGYLWFYLVYSGLTSSLTN
                     SKSGVVKIFLANLLGDALVFVGGILSLHFLAGMAFEKALVVGVLPFIIPDLGKLLAIS
                     FISRPLLQRLKNQAYFTN"
     gene            complement(40157..41503)
                     /gene="gor"
                     /locus_tag="VYN69_000048"
     CDS             complement(40157..41503)
                     /gene="gor"
                     /locus_tag="VYN69_000048"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEV1532409.1"
                     /translation="MREYDIIAIGGGSGGIATMNRAGEHGAKAAVIEEKKLGGTCVNV
                     GCVPKKIMWYGAQIAETFHQFGEDYGFKTTDLNFDFATLRRNRESYIDRARSSYDGSF
                     KRNGVDLIEGHAEFVDSHTVSVNGELIRAKHIVIATGAHPSIPNIPGAELGGSSDNVF
                     AWEELPESVAILGAGYIAVELAGVLHTFGVKTDLFVRRDRPLRGFDSYIVEGLVKEME
                     RTNLPLHTHKVPVKLEKTTDGITIHFEDGTSHTASQVIWATGRRPNVKGLQLEKAGVT
                     LNERGFIQVDEYQNTVVEGIYALGDVTGEKELTPVAIKAGRTLSERLFNGKTTAKMDY
                     STIPTVVFSHPAIGTVGLTEEQAIKEYGQDQIKVYKSSFASMYSACTRNRQESRFKLI
                     TAASEEKVVGLHGIGYGVDEMIQGFAVAIKMGATKADFDATVAIHPTSSEEFVTMR"
     gene            41789..42988
                     /locus_tag="VYN69_000049"
     CDS             41789..42988
                     /locus_tag="VYN69_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HEV1532410.1"
                     /translation="MKKKNGKAKKWQLYAAIGAASVVVLGAGGILLFRQPSQTALKDE
                     PTHLVVAKEGSVASSVLLSGTVTAKNEQYVYFDASKGDLDEILVSVGDKVSEGQALVK
                     YSSSEAQAAYDSASRAVAKADRHINELNQARNEAASAPAPQLPAPVGGEDATVQSPTP
                     VAGNSVASIDAQLGDARDARADAAAQLSKAQSQLDATTVLSTLEGTVVEVNSNVSKSP
                     TGASQVMVHIVSNENLQVKGELSEYNLANLSVGQEVSFTSKVYPDKKWTGKLSYISDY
                     PKNNGEAASPAAGNNTGSKYPYTIDVTGEVGDLKQGFSVNIEVKSKTKAILVPVSSLV
                     MDDSKNYVWIVDEQQKAKKVEVSLGNADAENQEITSGLTNGAKVISNPTSSLEEGKEV
                     KADEATN"
     gene            42972..43673
                     /locus_tag="VYN69_000050"
     CDS             42972..43673
                     /locus_tag="VYN69_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1532411.1"
                     /translation="MKQLISLKNIFRSYRNGDQELQVLKNINLEVNEGEFVAIMGPSG
                     SGKSTLMNTIGMLDTPTSGEYYLEGQEVAGLGEKQLAKVRNQQIGFVFQQFFLLSKLN
                     ALQNVELPLIYAGVSSSKRRKLAEEYLDKVELTERSHHLPSELSGGQKQRVAIARALV
                     NNPSIILADEPTGALDTKTGNQIMQLLVDLNKEGKTIIMVTHEPEIAAYAKRQIVIRD
                     GVISSDSAQLGKEEN"
     gene            43675..44934
                     /locus_tag="VYN69_000051"
     CDS             43675..44934
                     /locus_tag="VYN69_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1532412.1"
                     /translation="MQNLKFAFSSIMAHKMRSLLTMIGIIIGVSSVVVIMALGDSLSR
                     QVNKDMTKSQKNISVFFSPKKSKDGSFTQKQSAFTVSGKEEEVPVEPPKPQESWVQEA
                     AKLKGVDSYYVTNSTNAILTYQDKKVENANLTGGNRTYMDAVKNEIIAGRSLREQDFK
                     EFASVILLDEELSISLFESPQEAINKVVEVNGFSYRVIGVYTSPEAKRSKIYGFGGLP
                     ITTNISLAANFNVDEIANIVFRVNDTSLTPTLGPELARKMTELAGLQQGEYQVADESL
                     VFAEIQQSFSFMTTIISSIAGISLFVGGTGVMNIMLVSVTERTREIGLRKALGATRAN
                     ILIQFLIESMILTLLGGLIGLTIASGLTALAGLLLQGLIEGIEVGVSIPVALFSLAVS
                     ASVGMIFGVLPANKASKLDPIEALRYE"
     gene            45058..47055
                     /gene="metG"
                     /locus_tag="VYN69_000052"
     CDS             45058..47055
                     /gene="metG"
                     /locus_tag="VYN69_000052"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEV1532413.1"
                     /translation="MSEKNFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRLMGYDV
                     FYLTGLDEHGQKIQQKAEEAGITPQAYVDGMAVGVKELWQLLDISYDKFIRTTDDYHE
                     KVVAQVFERLLAQDDIYLGEYSGWYSVSDEEFFTESQLAEVFRDEAGNVTGGIAPSGH
                     EVEWVSEESYFLRLSKYQDRLVEFFKAHPEFITPDGRLNEMLRNFIEPGLEDLAVSRT
                     TFTWGVPVPSNPKHVVYVWIDALLNYATALGYAQDEHGNFDKFWNGTVFHMVGKDILR
                     FHSIYWPILLMMLDVKLPDRLIAHGWFVMKDGKMSKSKGNVVYPEMLVERYGLDPLRY
                     YLMRNLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVSMINKYFDGQIPAYVEGVT
                     EFDHVLAEVAEQSIADFHTHMEAVDYPRALEAVWTLISRTNKYIDETAPWVLDKDEAL
                     RDQLASVMSHLAASIRVVAHLIEPFMMETSRAVLTQLGLEEVSSLENLSLADFPADVT
                     VVAKGTPIFPRLNMEEEIAYIKEQMEGNKPAVEKEWNPDEVELKLNKDEIKFEDFDKV
                     EIRVAEVKEVSKVEGSDKLLQFRLDAGDGEDRQILSGIAKYYPNEQELVGKKVQIVAN
                     LKPRKMMKKYVSQGMILSAEHDGKLTLLTVDPAVPNGSVIG"
     gene            47431..47751
                     /locus_tag="VYN69_000053"
     CDS             47431..47751
                     /locus_tag="VYN69_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1532414.1"
                     /translation="MKETQLLKGVLEGCVLDMIGQKERYGYELVQTLREAGFDTIVPG
                     TIYPLLQKLEKNQWIRGDMRPSPDGPDRKYFSLMKEGEERVSVFWQQWDDLSQKVEGI
                     KNGG"
     gene            47755..48570
                     /locus_tag="VYN69_000054"
     CDS             47755..48570
                     /locus_tag="VYN69_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532415.1"
                     /translation="MKKMKYYEETSALLHEFSEENQKYFEELWESFNLAGFLYDEDYL
                     REQIYLMMLDFSEAERDGMSAEDYLGKNPKKIMKEILKGAPRSSIKESLLTPILVLAV
                     LRYYQLLSDFSKGPLLTVNLLTFLGQLLIFLIGFGLVATILRRSLVQDSPKMKIGTYI
                     VVGTIVLLVVLGYVGMASFIQEGAFYIPAPWDSLSVFTISLVIGIWNWKEAVFRPFVS
                     MIIAHLVVGSLLRYYEWMGISNVFLTKVIPLAVLFIGIFVLFRGFKKIKWSEV"
     gene            48645..49571
                     /locus_tag="VYN69_000055"
     CDS             48645..49571
                     /locus_tag="VYN69_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HEV1532416.1"
                     /translation="MRYITLGQDDKELSEIVLGMMRIKDKSVKEVEELVETALSVGIN
                     AFDLADIYGRGRCEELLGLVLKNRPDLREKMWIQSKCGIRIEEFTYFDFSKDYIIKSV
                     DGILQRLKIDHLDSLLLHRPDALMESDQVAEAFDLLYKQGKVRDFGVSNQNPMMMELL
                     KKDVKQPLAVNQLQLSAAFTPGFESAFHVNMEDSQAAMRDGSIFEYCQLHDVVIQAWS
                     VLQFGYFKGNFVGNEKFQALNQVLDRLAIKYGVTSSTIAISWILRYPAKMQAVVGTTN
                     PKHLREVSRAANFSLTRKEWYEIYLAAGNNLP"
     gene            49637..49903
                     /locus_tag="VYN69_000056"
     CDS             49637..49903
                     /locus_tag="VYN69_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEV1532417.1"
                     /translation="MKNVELKEKNMTFEEILPGLKAKRKYVRTGWGGAENYVQLFDTI
                     EQNGLALEMTPYFLINVSGEGEGFSMWSPTVCDVLATDWVEVHD"
     gene            49896..50594
                     /locus_tag="VYN69_000057"
     CDS             49896..50594
                     /locus_tag="VYN69_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEV1532418.1"
                     /translation="MTKRVLITGVSSGIGLAQARLFLEKGYQVYGVDQGEKSLLEGDF
                     HFLQRDLTLDLEPIFDWCPQVDVLCNTAGVLDDYKPLLEQTAQDIQAIFEINYIIPVE
                     LTRYYLTQMLENKKGIIINMCSIASSLAGGGGHAYTSSKHALAGFTKQLALDYAEAGI
                     QVFGIAPGAVKTAMTAADFEPGGLADWVASETPIKRWIEPEEIAELSLFLASGKASAM
                     QGQILTIDGGWSLK"
     gene            50605..51105
                     /locus_tag="VYN69_000058"
     CDS             50605..51105
                     /locus_tag="VYN69_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1532419.1"
                     /translation="MEIKKHFGVYAVCLENGKLLCIEKTRGPYQHRYDLPGGSQQLGE
                     GLTETLTREVMEETGFTVRSYSNPRIYDVFVREELKNFMVHHVMALYDVEMNESAPQV
                     TTSEAVSDGANDSLGYIWMDIQEITEENASPLVLKVKSELLGFPELDKTSYMNWKVND
                     EKSTCP"
     gene            51083..51952
                     /locus_tag="VYN69_000059"
     CDS             51083..51952
                     /locus_tag="VYN69_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme, TIM barrel domain protein"
                     /protein_id="HEV1532420.1"
                     /translation="MKNQLALSGEKILEKIYPQLFHHVGMIRGEYLLRELNQNILLQS
                     CQEFVKDYLETICSLYSDEEVWYRFTELTNTEANCLVGTKEFFDEGHPLFGYRGTRRL
                     LACLDEFQAEAHVVTEVYQTNPNLSVIFPFVNDADQLKQAITVLRQYGFTGKVGTMIE
                     LPSAYFDLSSILETGISKIVVGMNDLTSFVFATMRNSQWHDLESPIMLDMLRDMQDKA
                     RKNKINFAVAGYLNTSFIQKMNQLGIKCIIHYSSIPEIFDLEIDHPDHLKHIKEESKK
                     LQRSTHDTARNVE"
     gene            51927..52367
                     /locus_tag="VYN69_000060"
     CDS             51927..52367
                     /locus_tag="VYN69_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEV1532421.1"
                     /translation="MTPQEMWNKYKQINPSIGDEIDAWAFGVEPDLLADLVFKGEKTA
                     TASAYDLYVLEDEPLPQVGTFDVILDSQNQAVCIVEITKVSVKPFNRVSADHAYKEGE
                     GNKTLVYWRQVHEDFFRDCLGEAGLTFTPESKVVLEEFRKVYPL"
     gene            52490..55036
                     /locus_tag="VYN69_000061"
     CDS             52490..55036
                     /locus_tag="VYN69_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001149089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HEV1532422.1"
                     /translation="MQAVEHFIKQFVPEHYDLFLDLSRETKTFSGKVTITGQAQSDRI
                     SLHQKDLEITSVEVAGQARPFTVDHDNEALHIELAEAGQVELVLAFSGKITDNMTGIY
                     PSYYTVDGVKKEVLSTQFESHFAREAFPCVDEPEAKATFDLALRFDQAAGELALSNMP
                     EIDVDNRKETGIWKFETTPRMSSYLLAFVAGDLQGVTAKTKNGTLVGVYSTKAHPLSN
                     LDFSLDIAVRSIEFYEDYYGVKYPIPQSLHIALPDFSAGAMENWGLVTYREVYLVVDE
                     NSTFASRQQVALVVAHELAHQWFGNLVTMKWWDDLWLNESFANMMEYVCVDTIEPSWN
                     IFEDFQTGGVPLALERDATDGVQSVHVEVKHPDEINTLFDGAIVYAKGSRLMHMLRRW
                     LGDADFAKGLHAYFEKHQYSNTIGSDLWDALGQASGRDVAAFMDSWLEQPGYPVLTVK
                     VENDVLKISQKQFFIGENEDKNRLWVVPLNSNWKGLPDTLETESIEIPGYAALLAENE
                     GALRLNTENTAHYITDYQGDLLEAVLAELETLDNTSKLQIVQERRLLAEAGHISYADL
                     LPVLDKLAKEESYLVVSAVSQVISALERFIDEGTDAETAFKGLVAKLARHNYDRLGFE
                     AKDGESDEDELVRQLAVSMMIRSNDAEASQVASQIFATHKENLAGLPAAIRSQVLINE
                     MKHHETKDLLALYLDTYTHATDAVFKRQLAAALAYSTDADNIQNLITSWKDKFVVKPQ
                     DLSAWYYQFLAHQATQKTAWSWARENWAWIKAALGGDMSFDSFVILPAHVFKTQQRLA
                     EYKEFFEPQLSDLALSRNIGMGIKEIAARVDLISREKAAVEAVVLQYGNA"
     gene            55145..55819
                     /gene="ciaR"
                     /locus_tag="VYN69_000062"
     CDS             55145..55819
                     /gene="ciaR"
                     /locus_tag="VYN69_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CiaR"
                     /protein_id="HEV1532423.1"
                     /translation="MIKILLVEDDLGLSNSVFDFLDDFADVMQVFDGEEGLYEAESGV
                     YDLILLDLMLPEKNGFQVLKELREKGITTPVLIMTAKESLDDKGHGFELGADDYLTKP
                     FYLEELKMRIQALLKRSGKFNENTLTYGNIVVNLSTNTVKVEDTPVELLGKEFDLLVY
                     FLQNQNVILPKTQIFDRLWGFDSDTTISVVEVYVSKVRKKLKGTTFAENLQTLRSVGY
                     LLKDVQ"
     gene            55809..57143
                     /gene="ciaH"
                     /locus_tag="VYN69_000063"
     CDS             55809..57143
                     /gene="ciaH"
                     /locus_tag="VYN69_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000491790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CiaH"
                     /protein_id="HEV1532424.1"
                     /translation="MFSKLKKTWYADDFSYFIRNFGVFTLIFSTMTLIILQVMHSSLY
                     TSVDDKLHGLSENPQAVIQLAINRATEEIKDLENARADASKVEIKPNVSSNTEVILFD
                     KDFTQLLSGNRFLGLDKIKLEKKELGHIYQIQVFNSYGQEEIYRVILMETNISSVSTN
                     IKYAAVLINTSQLEQASQKHEQLIVVVMASFWILSLLASLYLARVSVRPLLESMQKQQ
                     SFVENASHELRTPLAVLQNRLETLFRKPEATIMDVSESIASSLEEVRNMRFLTTSLLN
                     LARRDDGIKPELAEVPTSFFNTTFTNYEMIASENNRVFRFENRIHRTIVTDQLLLKQL
                     MTILFDNAVKYTEEDGEIDFLISATDRNLYLLVSDNGIGISTEDKKKIFDRFYRVDKA
                     RTRQKGGFGLGLSLAKQIVDALKGTVTVKDNKPKGTIFEVKIAIQTPSKKKK"
     gene            complement(57177..57464)
                     /locus_tag="VYN69_000064"
     CDS             complement(57177..57464)
                     /locus_tag="VYN69_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3270 domain-containing protein"
                     /protein_id="HEV1532425.1"
                     /translation="MPVRKLQSYEVDYQEELNQQLPHYQAYTPEAQSDANLKEILFFI
                     NIAVFCICIAIFSFIFLALKLSTALAFAAAIGFSLLVLKVQRSIIKRKRRK"
     gene            57604..58533
                     /locus_tag="VYN69_000065"
     CDS             57604..58533
                     /locus_tag="VYN69_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006154193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1532426.1"
                     /translation="MEKIVITATAESIEQVEQLLEAGVDRIYVGEKDFGLRLPTTFSY
                     DQLREIAKLVHDAGKELIVAVNALMHQDMMDRIKPFLDLLEEIKTDYITIGDAGVFYV
                     VNRDGYSFKTIYDASTMVTSSRQINFWGQKAGASEAVLAREIPSAELFKMPEILEIPA
                     EVLVYGASVIHHSKRPLLQNYYNFTHIDDEKTHKRDLFLAEPSDPESHYSIFEDNHGT
                     HIFANNDLDLMIKLTELVEHGFTRWKLEGLYTPGQNFVEIAKLFIQARSLIQEGNFSH
                     DQAFLLDEEVRKLHPKNRFLDTGFYDYDPDMVR"
     gene            58733..61183
                     /locus_tag="VYN69_000066"
     CDS             58733..61183
                     /locus_tag="VYN69_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DnaQ family
                     exonuclease/ATP-dependent helicase"
                     /protein_id="HEV1532427.1"
                     /translation="MKTIGNRYVVVDLEATSTGSKAKIIQVGIVVIEDGEIVDHYTTD
                     VNPHEPLDAHIKELTGLTDQRLAQAPDFSQVARKIFDLVEDGIFVAHNVQFDANLLAE
                     NLFFEGYELRNPRVDTVELAQVFFPELEKYSLPILCRELGIPLKHAHTALSDAQATAE
                     LLLFLREKMTQLPKGLLERLLEMADALLYESYLVIEETYRNQSILSSPDLVQVQGLYF
                     KKTEASLEPRKLSQDFSKNISLLNLEVREEQESFAKEVGLLLKDEPVSLIQAPTGIGK
                     TYGYLLPALSQSKERQIVLSVPTKILQNQIMEEEGKRLKEVFHTDIHSLKGPQNYLKL
                     DAFYHSLQENDENRLFRRFKMQVLVWLTETETGDLDEIGQLYRYQHFLADLRHDGNLS
                     SQSLFVTEDFWKRSQERAETCKLLVTNHAYLVTRLEDNPEFVSDRLLIIDEVQKILLA
                     LENLLQETYDIQSIIDLIDKALVGEENRVQQRILESIRFECLYLIEQFQSGKSRKNIL
                     DSLDNLHQYFSELEVEDFDELVRYFTAEGDYWLEVTETSQKKIQISSTKSGRTLLSSL
                     LPESCQVLGVSATLEISQRVSLADLLGYPEAKFVKIESRGKQEQEVVMVKDFPLVTET
                     SLEVYAREVAALLVEIQAFQQPILVLFTAKDMLLAVSDLLTVSHLAQYKNGDVHQLKK
                     RFEKGEQQILLGAASFWEGVDFSSHPSVIQVVPRLPFQNPQEPLTKKINQELNQEGKN
                     AFYDYQLPMAIIRLKQALGRSMRREYQRSLTLILDRRIVGKRYGKQIVASLAEEATVK
                     TISRSEVDEAIDRFFNEL"
     gene            61220..62443
                     /locus_tag="VYN69_000067"
     CDS             61220..62443
                     /locus_tag="VYN69_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1532428.1"
                     /translation="MKRSLDSRVDYSLLLPVFFLLVIGVVAIYIAVSHDYPNNILPIL
                     GQQVAWIALGLVIGFVVMLFNTEFLWKVTPFLYILGLGLMILPIVFYNPSLVASTGAK
                     NWVSINGITLFQPSEFMKISYILMLARVIVQFTKKHKEWRRTVPLDFLLIFWMILFTI
                     PVLVLLALQSDLGTALVFVAIFSGIVLLSGVSWKIIIPVFVTAVTGVAGFLAIFISKD
                     GRAFLHQIGMPTYQINRILAWLNPFEFAQTTTYQQAQGQIAIGSGGLFGQGFNASNLL
                     IPVRESDMIFTVIAEDFGFIGSVLVIALYLMLIYRMLKITLKSNNQFYTYISTGLIMM
                     LLFHIFENIGAVTGLLPLTGIPLPFISQGGSAIISNLIGVGLLLSMSYQTNLAEEKSG
                     KVPFKRKKVVLKQIK"
     gene            62454..63008
                     /locus_tag="VYN69_000068"
     CDS             62454..63008
                     /locus_tag="VYN69_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HEV1532429.1"
                     /translation="MVKVAVMLAQGFEEIEALTVVDVLRRANITCDMVGFEEQVTGSH
                     AIQVRADHVFDGDLSDYDMIVLPGGMPGSAHLRDNQTLIQELQSFEQEGKKLAAICAA
                     PIALNQAEILKNKRYTCYDGVQEQILDGHYVKETVVVDGQLTTSRGPSTALAFAYELV
                     EQLGGDAESLRTGMLYRDVFGKNQ"
     gene            63093..63656
                     /locus_tag="VYN69_000069"
     CDS             63093..63656
                     /locus_tag="VYN69_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1532430.1"
                     /translation="MKYHDYIWDLGGTLLDNYETSTAAFVETLALYGITQDHDSVYQA
                     LKVSTPFAIETFAPNLENFLEKYKENEARELEHPILFEGVSDLLEDISNQGGRHFLVS
                     HRNDQVLEILEKTSIAAYFTEVVTSSSGFKRKPNPESMLYLREKYQISSGLVIGDRPI
                     DIEAGQAAGLDTHLFTSIVNLRQVLDI"
     gene            63671..65617
                     /gene="gyrB"
                     /locus_tag="VYN69_000070"
     CDS             63671..65617
                     /gene="gyrB"
                     /locus_tag="VYN69_000070"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002283665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEV1532431.1"
                     /translation="MTEEIKNLQAQDYDASQIQVLEGLEAVRMRPGMYIGSTSKEGLH
                     HLVWEIVDNSIDEALAGFSSHIQVFIEPDDSITVVDDGRGIPVDIQEKTGRPAVETVF
                     TVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVHVHKNGKIHYQEYRRGHVVAD
                     LEVVGDTDRTGTTVHFIPDPKIFTETTTFDFDKLNKRIQELAFLNRGLQISITDKRQG
                     LEQTKHYHYEGGIASYVEYINENKDVIFDTPIYTDGEMDDITVEVAMQYTTGYHENVM
                     SFANNIHTHEGGTHEQGFRTALTRVINDYARKNKLLKDNEDNLTGEDVREGLTAVISV
                     KHPNPQFEGQTKTKLGNSEVVKITNRLFSEAFSDFLMENPQIAKRIVEKGILAAKARV
                     AAKRAREVTRKKSGLEISNLPGKLADCSSNNPAETELFIVEGDSAGGSAKSGRNREFQ
                     AILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGAEFDVSKARYQKLVLMTDAD
                     VDGAHIRTLLLTLIYRYMKPILEAGYVYIAQPPIYGVKVGSEIKEYIQPGADQEIKLQ
                     EALARYSEGRTKPTIQRYKGLGEMDDHQLWETTMDPEHRLMARVSVDDAAEADKIFDM
                     LMGDRVEPRREFIEENAVYSTLDV"
     gene            65699..67426
                     /gene="ezrA"
                     /locus_tag="VYN69_000071"
     CDS             65699..67426
                     /gene="ezrA"
                     /locus_tag="VYN69_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEV1532432.1"
                     /translation="MSNGQLIYLMVAIAVILVLAYVVAIFLRKRNEGRLEALEERKEE
                     LYNLPVNDEVEAVKNMHLIGQSQVAFREWNQKWVDLSLNSFADIENNLFEAEGYNHSF
                     RFLKASHQIDQIESQITLIEEDIAAIRNALADLEKQESKNSGRVLHALDLFEELQHRV
                     AENSEQYGQALDEIEKQLENIQSEFSQFVTLNSSGDPVEAAVILDNTENHILALSHIV
                     DRVPALVTTLSTELPDQLQDLESGYRKLIDANYHFVETDIEARFHLLYEAFKKNQENI
                     RQLELDNAEYENGQAQEEINALYDIFTREIAAQKVVENLLATLPTYLQHMKENNTLLG
                     EDIARLNKTYLLPETAASHVRRIQTELESFEAAIVEVTSNQEEPTQAYSVLEENLEDL
                     QTQLKDIEDEQISVSERLTQIEKDDINARQKANVYVNRLHTIKRYMEKRNLPGIPQTF
                     LKLFFTASNNTEDLMVELEQKMINIESVTRVLEIATNDMEALETETYNIVQYATLTEQ
                     LLQYSNRYRSFDERIQEAFNEALDIFEKEFDYHASFDKISQALEVAEPGVTNRFVTSY
                     EKTRETIRF"
BASE COUNT        20701 a        12408 c        14716 g        19916 t
ORIGIN      
        1 tacttctaaa acatttttag aaatcgattt gactgtcctg atcgatttgc cctgttcttg
       61 tttcgtttta ctatagaacg atttgaaggc gtttatgata tttagctgta cgagagtctt
      121 ttaaaagtgt tttgatggct tggatttctt ctttagttga tttcatatca ctattatata
      181 atgctttttg attttagttt agtattatcg tagaaaatgg actcggtgct atggatacac
      241 cggatgagaa tggttatgta gcagataact atcggattac ttacttagag gcccacatca
      301 aggccatgcg agatgccatt taccaagacg gggttgactt gcttggttat acgacttgga
      361 gctgtatcga tccggtttca gctggcacag gggaaatgaa taagcgctat ggctttatct
      421 atgtagatcg agataatgta ggaaacggag ctctcaaacg tagcaagaag aagtccttct
      481 attggtacaa ggatgtcatt gatagcaatg gtgcaagcat cgggtagagc acatttgttt
      541 actattttta taaaatcttc tccctgcttt ataagatgta aaaaagagtt caattcgaac
      601 tctttttttg ctataatgag gggagaaaaa tcagacagga gatcgacatg tcagaaccat
      661 tatttttaca atcagttatg caagaaaaaa tctggggtgg agccaagcta cgtgatgagt
      721 ttggctacga tatcccaagt gaaaaaatcg gagaatattg ggccatctca gcccatccaa
      781 atggagtctc taaagttgcc aatggtcgtt acgagggaac agatcttgct actttgtatg
      841 cggaacaccg tgaattattt ggcaatcgtc cagaacctgt atttccactt ttgaccaaga
      901 tcctcgatgc caacgactgg ctcagtgtcc aagttcaccc agacgatgct tatggactcg
      961 agcatgaagg cgaactcgga aaaacagaat gctggtacat tatcgcagcg gatgaaggtt
     1021 cagagattat ctacggtcac aatgccaagt caaaagaaga actccgccag caaatcgagg
     1081 ataagaactg ggatgcgctg ttgacaaaag tgccagttaa agcaggagat ttcttctatg
     1141 taccaagcgg taccatgcac gctatcgggg cgggtatctt gattcttgaa acccagcagt
     1201 ctagcgatac cacttaccgt gtctatgact ttgaccgtaa ggatgacaag ggcaatttac
     1261 gtgaacttca ccttgaaaaa tctatcgatg tcttgaacat tggtgaacct gcaaatagcc
     1321 gtccagtgac aatcaaagca gatgatctac gttccactct ccttgtatcc aatgatttct
     1381 tcgcagttta caaatgggaa attactggaa aagttaactt tgaaaagaca gctgactaca
     1441 gcttgttgag tgtcttggct ggtcaaggtc aactaactgt tgacgggaaa aactatccaa
     1501 tccaaaaagg gagacacttt atcctaccaa gtgatgttga atcttggact ctagaagggc
     1561 aagatttaga attaattgtt agtcatccat aaaaaagaaa ggacctgagt tcattactca
     1621 agtccttttg attacataaa ttgggcgatg aagaccacga tgatgattgg aatgacgaaa
     1681 cgaagaagga agagccagac ttggaacagt ccttgtttcc atgctctttc atcgagatgg
     1741 agttcctcca ttgcaagagc ctttttaaag atatagcctg taaaaagtga aaggaagaga
     1801 gctccaaatg gcatgaggag attggaaacc aagaagtcca tagcgtcaaa gaaggcttta
     1861 ccaaaaatgt gaacatccgc catgacaccg taagataggg ctgaaggaat gccaaagaca
     1921 aaggtcaaaa ttcctaaaat aacactccat ttagcacgct tgctgttgtc ctgattggtg
     1981 atattgtcta cattgatttc cagcataacg acagaagaag tgacagtcgc aaagaggaag
     2041 aggacataga aaatggttcc aaaaggcatc ttgtcaaaga gttgaggcaa gacgataaag
     2101 accaagctcg gtcccccttc agactggata ttgaaggggg accgagcttg gaaaatggct
     2161 agacctgcca tgatggatac cgagatattc atggctacga tggagattct tgactggacc
     2221 agattggttt tcttgtccaa gtaagaagca taggtcaaca tggctgtaac ccctagtgag
     2281 agggcaaaga aagattgtct cagagcatag aggagaccag cgctagtcag ttttgaaaaa
     2341 tctggtttga ggaagtaaag aaccccttcc atggcatttg gcaaactgag agagcgttcg
     2401 atgatgacga caaagatgat aaagagcagg ggcatcatga ctttcgaagc tctttcaatc
     2461 cctttttgaa ccccacgtga tacaatgaag atattcaata ggataaaggt cgcttgagct
     2521 cctagggcaa tggctggatt tgaaatgatt gaagtaaata actgagcata atcacccgtt
     2581 ccaccaagtt ggaacaattt cccaaactca atacctagat agactagaat ccatcctccg
     2641 ataacactgt aaaaagataa gaggataaag agggcaaagg cgccaatcca accgataaag
     2701 ttgtacttgt tattcttgcc cagttttcca aaggttttga tagcggaaac gccagcacta
     2761 cggccaaggg caaactcagc cagcaggaga gggaaatcga ttaaaatagt ggaaatgaga
     2821 aagattagta aaaagcctcc accgccatta gcagcagtca tgtagggaaa cttccaaacg
     2881 gaaccaagcc cgatgaccga gccagcagat gctagaataa agccaagttt cgacccccat
     2941 tgtgattttt ctgacatagt taaactctga tctccttttt caaaataaga aaaagctact
     3001 tgagttgtca aatagccttc tgtttgattg atagacttga ctatcctatc atgttttcta
     3061 aggtctgtca agaaaaccat tttcaagttt tcacaccttt ctcaaaaacg cttgactctg
     3121 acctaagggg aggggttata ctatcattgt aagaaggaaa tcatgtacca tataaaagaa
     3181 gctgcgcagc tttcaggtgt ctctgtcaag accctgccct gcatcactat gacaagatag
     3241 gactcttggt ccccttaaag tcggaaaacg gctatcgaac ctatagtcaa gaggatttgg
     3301 aacgccttta ggtcattctt tactacaaat atctaggctt ttctttagag aaaatagcag
     3361 agctgttaaa ggaagaaagg acagatttat tgccccattt aactaggcag ttggactatc
     3421 taactcgcga aaggcaacat ctggatacct tgatttccac cttgcaaaaa actattcaag
     3481 aacaaaaagg agaaagaaaa atgaccattg aggaaaaatt cacgggattt agctatcaag
     3541 acaatcaaaa atatcaccaa gaagcggtag agaaatatgg tcaagaagtc atgggacaag
     3601 cgctcgaacg ccaaaaaggt cacgaagacg aggctacggc cgcctttaac caagtctttc
     3661 aaactttggc acaaaatctt caagttggtt tacctgcaac agcaaccgaa aaccaagagc
     3721 aagcagccaa gctcttgcaa gctattcgca cttatggatt tgactgctct attgaggtat
     3781 tcggtcatat cggtaaaggt tacgtctaca acccagagtt taaggaaaac attgacaagt
     3841 ttggttctgg aacagcccag tacacgtcag atgccattgc ggcttacgtt cagacaaatg
     3901 cagaataaat agactaggaa tttcctcaat gtcattaagt taagaaattc aaatacaatt
     3961 ctgtatttgg gtctcttttt tatgtgataa actatagtta aggaggtatt tcccatgata
     4021 aaacagataa aagcccactt gaataagagt attcagagta tcattggcca aaaagttgag
     4081 ttcgtcaaac aagatgaaca ggcctttact cgtaaaagaa ggttatcact agaaactatg
     4141 attcgtacaa ttctgggcat gggaggaaaa tcactatcaa aagaattact agatgccaga
     4201 ctgacagttt caaattcagc ctttgttcaa agacgctatc agataaaacc tgaagctttc
     4261 tatgctctat ttaaagaatt tacagcacct attccactta atactgattt tccaatattc
     4321 gctgcagatg ggagtgatat ctgtattcct cgaaatccca tggatacaga aacctctatc
     4381 caaacccaaa cggatgttaa atcctataat ctcatacaca taaatgccct atacgacttg
     4441 acgactggag tgtatagaga tgtctctatt caagacaaac atgcacaaca tgagcgctta
     4501 gctctgattc agatgatgga ggcttctccc tttcgagaaa gctcttgtta tcatggatag
     4561 aggctatgaa agttacaatc tcatggctca ttttcaagaa aaaggttggc tttatatcat
     4621 ccgtattcga gatggaaaac aatccatgcc ttcctccttc aacttgccaa atacggagtg
     4681 ttttgatcaa aaagtttctc taaaattatc acgaaagcaa actaatcagc tcaaaaaact
     4741 ttatcgtgat tttcccaatg actatcattt tattccacac aattctattt ttgattttct
     4801 tccagaaaca agtcgaaaac aagatcctgt aacactctat gaacttcctt ttcgtatggt
     4861 gcgtttagaa gtggaagaag gaaaatacga aactttagtg acgaatacag actattcagt
     4921 ccaagaatta aaaaatctct acgccagtag atggggcata gagactagtt ttcgtgacct
     4981 gaaatacagt attggcttgg tcaattttca cgccaaaaag aagggaggga ttctccaaga
     5041 aatctttgct cgctttacaa attttaactt ttgtcgttgg gtaacctcgc aagttgccat
     5101 cgacagtagt cacaaaaaac aaagatataa agtgtgtttc tcagatgcgg cttatgcctg
     5161 ccgtttgttt tttaacggtt ccctttcttc cctccagttg aaaaactacc tcaagaaaca
     5221 gttatctatt attcgaccga atcgaaaata ttcaagaaag ataaaagctc aatcggtagt
     5281 tgatttcatc tatagagtaa cataaatctc aaaaaatagg gtgaaaaaac ctttatttgt
     5341 catgcaaaaa aatagaagat gtgatagaga atcactttga tgatctcaat cacacttcct
     5401 attcaagtag ttagatcact aacttaatga cattgggaat ttcctagcct attttttact
     5461 tcaaatcata aagccagtcg tcaccgtttt tgtagtaaaa gaattcactg agatcttctt
     5521 ctagaaacac acgaagcata tcagacatat catcggttgc aagttttaga tgagaaagat
     5581 tttcaaagtc ctcccaccaa actttccctt cgtctgaaga ctggagttca ccagtaaagt
     5641 gttctgtctt gtaaaaaagg acgacataac gataatcctt gtcgtcatac cagtttttga
     5701 taccacagag ttggggtttg gaaatgatca gaccagtttc ttctttcact tcacgaatga
     5761 cagcatcgac aaaggattcg ccacgttcaa catgaccacc aggaaaagta atgccagacc
     5821 agtcgggact aactcggtct tggaccagga ccttatctcc gtttttaatc atacacatgt
     5881 taacaaattc gactgcctct cttctgttca ttcttcacaa cctttaatct ttaatcataa
     5941 tgcagacttc ccgccaccca gccggtacag agggcagaag tgatgttaaa gccacccgtg
     6001 tgggcattga tatccataac ttcgcctgca aagtggaggc caggtaccag cttactttca
     6061 agggttttag gattgatttc cttgagactg actccaccct tggtaacaaa ggactttgca
     6121 agggacattt ttccagttac aggaatttta agttctttaa tggactggac aagttgttct
     6181 cgttcctttt cagtcagctg tttgactttt tcaggatatc cttgtacaaa aaattcggcc
     6241 aagcgttctg gtaacaaggt ttttaaagcg tttttcaagg atttttcccg attttcttct
     6301 agaaatgtaa ccaagtcctt ctcagaaagt tgaggcaaaa catcgagtga gagaacctcc
     6361 ccacctttga caaagctaga catgcgtagg gcagcaggac ctgacaaacc aaagtgggta
     6421 aagagtaaat catgagtgat gacatgctta ccataactta gggtcacatc gtccagagaa
     6481 atcccttgta aggctttatg tggaaaatct gttaataaag gactttcagc agcctcaaga
     6541 tcggtgatgg tatgcttaaa atggcgagca atctcgtgac caaaaccagt cgaaccagtc
     6601 gaaggataag acttaccacc tgttgtgaca atgagtttct cacaagtgaa ggtttgatcc
     6661 gctgacttaa ggacaaactg gtcatctact tttttaacag aaacgatttc tatttgagta
     6721 gcaacttgac cacctagttc agtgattttc ttttccaaag cttcgataat agtccgagac
     6781 ttgtcactgg ctggaaagac gcgtccgtgg tcttcgacct taagtttaac accattttct
     6841 gtaaaaaagt tgatgatgtc atgattatcg aactgggaga aaacactgta aagaaagcgt
     6901 ccgtttccag gaattccagc tagcaggttg tctaagctac cattgttggt cacattgcaa
     6961 cgtcccccac cagtcccagc taattttttt ccaagtttcc gatttttttc gatgaggagg
     7021 gttttctgtc cataaaagct actggaaatc gtagccatca taccagcagg tcccccaccg
     7081 atgacaatag tatcaaaatg tttcatagct ctattgtacc acaaaaaaac aagagatgat
     7141 ggtcacctct tgtcaagaat acaattaatc aatttcatag cccatcagca aaccgccatc
     7201 ttctgcatag aaactgcaga gaccagaggt tggtagaatt ttaatatccg cttgtgggaa
     7261 ggtttcacgg attcgctctg agagttgttg acaacatttt tcgttattgc gttgggccat
     7321 gacaatacgg ccaccagcat atccagcttt tactaactca tcataggcag cttgaactga
     7381 tttctttgat ccccttgctt tttgtagcaa ttcgagagtt ccagtttcac tagcttttcc
     7441 gaccatacga atgttgagaa ggccaacgac cgtaccgata agcttgctca aacggccgtt
     7501 cttcaccaag ttatcgactt tggctaggac aaagagcaac ttagtttttt cttgataggc
     7561 ggtgatagct tcaaccactt cttcaaaaga caagccctgg tcaatcaagt cattcaattt
     7621 ttctacgagt aggtcaactt caccaccagc agataaacta tcaatcacat gaatcttagt
     7681 gtcaggatgg tcttccagat aaatattctt tgctagttga gcactattgt gactgccaga
     7741 aagggtacct gtgatggtta ctaggaaaat gtttttggca ccttcaaatg ctcgcaaata
     7801 gtcatctggg cttggacaag ccgattttga agcttctgca gttgcataca tggtttccat
     7861 catttggtca atatcgagac tggcgtcatc aacaaagacc tgatcagcta cttgaatggt
     7921 taaggggaca cttacaaagg ttgtgttaat agctggtgtt ggcagttgac gataatcaca
     7981 accagagtca gcaataatct tccaagtcat agaaattctc catctttgtc agttatacat
     8041 tgacaaaggt tctgtctttt tttacaatta tatcatgaaa acccttgaaa caaaagcatc
     8101 atccgtctgt tgtgacaagt agaaagaaaa tgttatgtct gaacgtagaa tctctgaaaa
     8161 gtctcttgaa aatctcagaa aatcaaacca agaatccaat ttattaacca gagaagccat
     8221 tgaaacagcc ctcttgcaac tcttggaaaa aaaggaactg accaagatta gtatttctga
     8281 attggtcaaa cgtgcaggtg tttcgcgtgc ggccttttat cgcaattatg actccaaaga
     8341 ggaaatttta gagagcgtct ttaaacgaac tgtccacaat attatggaac agctgcatca
     8401 ttacgattta aagacagacc tttatttggt ttgggttcac cttttccggg aggccagaaa
     8461 ggaagctaga gtaattcaat tggccttgga ttaccatctg gaaaaaatct ttgtccaagc
     8521 catgcaggaa tttctagaaa aataccatgg gaaatcaaaa ggtgtcagct cttatcttca
     8581 ttccttctgg agctcagcca tcgtctctgt ccttctaaaa tggatcaagg atggcatgaa
     8641 ggtaccagct gaaaagattg cagatttagg tttaccattt tttaaaaaat agagaaaaag
     8701 gagaaaagag atgactgaaa aaagactggc ctgggatgag tattttgcag cccaagctct
     8761 actaattgcg aatcgttcca cttgtaaacg tgctaaagtg ggcgcgattc tggtaaaaga
     8821 taataaggtt atttccactg gttacaatgg ttcggtgtca gggactgagc attgtattga
     8881 tcacgaatgt ttggtcattg aaggccactg tgttcgcacc cttcacgctg aggtcaatgc
     8941 tatccttcaa ggtgctgagc gtggtgttcc caaaggtttt acagcctatg taacccattt
     9001 tccttgtctc aactgtacaa aacaattgct tcaggtcggc tgcaagcgtg tggtttatat
     9061 caaccagtac cgaatggacg actacgccca atacctttat caagaaaaag ggacagaatt
     9121 gacccattta ccacttgaga cagtacagac agctcttaaa gaggctgatc taatgtaaaa
     9181 attatcaaaa ataaatggtt tagaaagatt tttaccccgt tttttggtat aataagaaga
     9241 ataaattgaa agaaggaatt ccaaaaatgg gaaaaattga agttattaat cacccactga
     9301 ttcaacacaa attgtcaatc ttgcgtcgta cagatacttc tacaaaagct tttcgtgagc
     9361 tagtagatga gattgcaatg ttgatggggt atgaagtact tcgtgatctt ccactagaag
     9421 atgtggaaat cgaaacacca attacaaaaa cagttcaaaa acaattggca ggtaagaaat
     9481 tggccatcgt cccaatcttg cgtgcaggta tcgggatggt tgatggtctc ttgagcttgg
     9541 ttccagctgc taaagttggc cacatcggta tgtaccgtga tgaagaaaca cttcaaccag
     9601 ttgaatactt ggtgaaattg cctgaggaca ttgaccaacg tcaaattttt gtagtagacc
     9661 caatgttggc aacaggtggc tcagcaatct tggctgttga ttctcttaaa aaacgtggcg
     9721 catcaaatat caaatttgtc tgccttgtat ctgctccaga gggtgtaaaa gcccttcaag
     9781 aagctcatcc agatgtagaa atctttacag cagccttgga tgaacgtttg aacgaacacg
     9841 gttatatcgt tccaggtctt ggagatgctg gagaccgctt gttcggtaca aaataagatc
     9901 gaaaagaaga atgacttgga aaagtatttc cagtcacgaa aggaggttgg gtttttgttt
     9961 ctgtctaatg aaagcagagc aaaaatttga ccttttttga ccaagatatt ataatagtct
    10021 tatcttcagt catttgacca acaaaattaa aactcaaaag gagaaatgaa tgattcctgt
    10081 agttattgaa caaacaagcc gtggagaacg ttcttacgat atttactcac gtcttctcaa
    10141 agaccgcatc attatgctga caggtccggt tgaagacaat atggctaact ctgttattgc
    10201 ccaactgctt ttcttggatg cccaagatag tacaaaagat atttaccttt atgtcaatac
    10261 accaggtggt tctgtttcag ctggtttggc aatcgtagat accatgaact ttatcaaggc
    10321 agatgtccaa accattgtta tgggaatggc tgcatctatg ggaacagtca tcgcatcaag
    10381 tggagcaaaa ggcaaacgtt tcatgcttcc aaatgctgaa tacatgattc accaaccaat
    10441 gggcggtaca ggtggtggta cccaacaaac tgatatggct atcgctgcag aacacttgct
    10501 caaaactcgt aataccttgg aaaaaatctt ggctgaaaat tcaggtcagt caatggaaaa
    10561 agtccatgca gatgcagaac gtgataactg gatgagctcc caggaaacac ttgaatatgg
    10621 ctttattgat gaaattatgg ccaacaattc attgaactaa tgatgataga aggcaaactc
    10681 gactgggttt gctttttttg gtataatagg gagagatttc ttagaaagag gatttatcat
    10741 gtttgaaaaa gtcaatcgct ctggcttgat tatctatctt tactataatc gtgatgccaa
    10801 aaaactgcag gattatggag atattaccta tcattccaag aaacatcgtt acttacaact
    10861 ctatgttcca actcaagaag tggagcaatt ggtcggacgc ttgagcaagg aaaaatttat
    10921 taaaaaagtt agggtttgtc atatccaaga cttggaaaca ccctttgtgg gcaatcttta
    10981 tcgataggaa aacgttatca tcgaaaaagt tcaaggaaaa cattgacaat tttctgataa
    11041 ttcggtatat tcttaacaga ctatttaaga aataaggaga caaaaagatg aagaaaaaat
    11101 ttgccctatc gtttgtggcg cttgcaagtg tagcacttct tgcagcctgt ggagaagtga
    11161 agtctggagc agtcaacact gctggtaact cagtagagga aaagacaatt aaaatcgggt
    11221 ttaactttga agaatcaggt tctttagctg catacggaac agctgaacaa aaaggtgccc
    11281 aattggctgt tgatgaaatc aatgccgcag gtggtatcga tggaaaacaa atcgaagtag
    11341 tcgataaaga taataagtct gaaacagctg aggctgcttc agttacaact aaccttgtaa
    11401 cccaatctaa agtatcagca gtcgtaggac ctgcgacatc tggtgcgact gcagctgcgg
    11461 tagcgaacgc tacaaaagca ggtgttccat tgatctcacc aagtgcgact caagatggat
    11521 tgactaaagg tcaagattac ctctttattg gaactttcca agatagcttc caaggaaaaa
    11581 ttatctcaaa ctatgtttct gaaaaattaa atgctaagaa agttgttctt tacactgaca
    11641 atgccagtga ctatgctaaa gggattgcca aatctttccg cgagtcatac aagggtgaaa
    11701 tcgttgcaga tgaaactttc gtagcaggtg acacagactt ccaagcagcc cttacaaaaa
    11761 tgaaagggaa agactttgat gctatcgttg ttcctggtta ctataatgag gctggtaaga
    11821 ttgtaaacca agcgcgtggt atgggaattg acaaaccaat cgttggtggt gatggattca
    11881 acggtgagga gtttgtacaa caagcaactg ctgaaaaagc atcaaacatc tactttatct
    11941 caggcttctc aactactgta gaagtttcag ctaaagctaa agccttcctt gacgcttacc
    12001 gtgctaagta caatgaagag ccttcaacat ttgcagcctt ggcttatgat tcagttcacc
    12061 ttgtagcaaa cgcagcaaaa ggtgctaaaa attcaggtga aatcaagaat aaccttgcta
    12121 aaacaaaaga ttttgaaggt gtaactggtc aaacaagctt cgatgcagac cacaacacag
    12181 tcaaaactgc ttacatgatg accatgaaca atggtaaagt tgaagcagca gaagttgtaa
    12241 aaccataata gaaaaatgtt gaaataggga atgagccttt gactcactcc ctgtttcgat
    12301 atttaatact cttcgaaaat ctcttcaaac tgcgtcaacg tcgccttgga ttatatatgt
    12361 gactgacttc gtcagtctta tctacaacct caaagcagtg ctttgagcaa cctgcggcta
    12421 gtttcctagt ttgctctttg attttcattg agtataagaa cctatcaaaa agtgagggaa
    12481 aaccctcgga attataaata gaaagagtga atcttatgct ccaacaactc gtaaatggtt
    12541 tgattctagg tagtgtttac gcgctgttag ccctaggata taccatggtt tacggaatta
    12601 tcaagctcat caacttcgcc catggtgata tttatatgat gggagccttt atcggttatt
    12661 tcttgatcaa ttctttccaa atgaatttct ttgtagcgct tattgtagct atgctagtga
    12721 cagctattct tggtgtcgtg attgagtttc ttgcttaccg acctttgcgc cactctactc
    12781 gtattgctgt tttgattacg gctattgggg tttctttcct attggagtat ggaatggtct
    12841 atctggttgg tgccaatacc cgtgccttcc ctcaagcgat tcaaacagtt cgatatgatt
    12901 tgggaccaat tagcttaaca aatgtgcagt taatgatttt ggccatttcc ttgattttga
    12961 tgattttgtt acaagtcatt gtccaaaaga ctaagatggg gaaagccatg cgtgcagtat
    13021 cagtagatag cgacgcggca caattgatgg ggatcaatgt aaaccgtacg attagcttta
    13081 ccttcgcttt gggttctgct cttgcgggtg cggctggtgt tctgattgct ctttattata
    13141 actctcttga gcctttgatg ggggttactc caggtcttaa atctttcgtt gccgcagtac
    13201 ttggtggtat cggaattatt cctggtgcgg ctcttggtgg ctttgtgatt ggtctattgg
    13261 aaacctttgc gacagccttt gggatgtcag atttccgtga tgccattgtt tatggaatct
    13321 tgttgttgat cttgattgtc cgcccagctg gtatccttgg taagaatgtg aaagagaagg
    13381 tgtaaacgat gaaggaaaat ttaaaagtta atattctatg gttactcctt ttgttagctg
    13441 gctatagctt gattagtgta ctggtttcag tcggagtact taatctattc tatgtacaga
    13501 ttttacaaca aattggaatt aatattattt tggctgttgg tctcaactta atcgttggtt
    13561 tttcaggaca attttcactt ggtcatgctg gtttcatggc gattggtgcc tatgcagcag
    13621 ctattattgg ttctaaatca ccaacctacg gtgccttctt tggagctatg cttgtagggg
    13681 ctttgctttc aggagcagtt gccttacttg tcggcattcc aaccttgcgc ttgaaggggg
    13741 actatcttgc ggtagcaact ctaggtgttt ctgaaattat ccgtatcttt atcatcaatg
    13801 gtggaagcct tacaaatggt gcggcaggta tcttagggat tcctaacttt acaacttggc
    13861 aaatggttta cttctttgtc gtgattacaa ccattgcaac cttgaacttc ttgcgtagcc
    13921 caattggtcg ttcaaccctc tctgttcgtg aagatgaaat cgctgctgag tcagttgggg
    13981 ttaatacgac taaaattaaa atcatcgctt ttgtctttgg tgccattact gcaagtattg
    14041 ctgggtcact tcaggcagga tttatcgggt ctgttgtacc gaaagattac accttcatca
    14101 actcaatcaa cgttttgatt attgttgtat ttggtggact cggttccatt acaggtgcga
    14161 ttgtttcggc tattgttctg ggaattttga atatgcttct ccaagatgtt gctagtgtgc
    14221 gtatgattat ttacgctttg gccttggtat tggtaatgat tttcagacca ggtggactcc
    14281 ttggaacatg ggaactgagc ctatcacgtt tctttaaaaa atctaagaag gaggaacaaa
    14341 actaatggca ttacttgaag taaaacagtt aaccaaacat tttggtggtc taacagctgt
    14401 tggagatgtg actcttgaat tgaacgaagg ggaactggtt ggattaatcg gtccaaacgg
    14461 agctgggaaa accacccttt tcaacctttt gaccggtgtt tatgaaccaa gtgagggaac
    14521 agtaactcta gatggtcacc ttttgaatgg gaaatcacct tataagattg cctctttggg
    14581 acttggacgt actttccaaa atatccgtct ctttaaagat ttaacagttt tagacaatgt
    14641 tttgattgct tttggaaacc atcacaaaca gcatgttttt actagtttct tacgcttacc
    14701 agctttttac aagagtgaaa aagaattaaa ggctaaagct ttggaattgt tgaaaatctt
    14761 tgatttagat ggtgatgcag agactcttgc taaaaatctt tcctacggac aacaacgtcg
    14821 tttggaaatt gttcgtgccc ttgctacgga acctaaaatt ctcttcttag atgaaccagc
    14881 agcaggtatg aacccacagg aaacagccga attgactgag ttaattcgtc gtatcaaaga
    14941 tgaatttaag attacaatca tgttgattga acacgatatg aatctggtca tggaagtaac
    15001 agaacgtatc tacgtacttg aatatggccg tttaattgct caaggaactc cagacgaaat
    15061 taagaccaat aaacgcgtta tcgaagctta tctaggaggt gaagcctaat gtctatgtta
    15121 aaagttgaaa atctttctgt gcattacggt atgatccaag cagtccgtga tgtaagcttt
    15181 gaagttaatg aaggagaagt tgtttccctt atcggtgcca acggtgcagg taagacaact
    15241 attcttcgca ccttgtcagg tttggttcga ccaagttcag gaaagattga atttttaggt
    15301 caagaaatcc aaaaaatgcc agctcagaaa atcgtggcag gtggtctttc acaagttcca
    15361 gaaggacgcc acgtctttcc tggcttgact gttatggaaa atcttgaaat gggagctttc
    15421 ttaaagaaaa atcgtgaaga aaatcaagct aacttgaaga aggttttctc acgctttcct
    15481 cgtcttgaag aacggaagaa ccaagatgca gctactcttt caggagggga acaacaaatg
    15541 cttgccatgg gacgcgctct tatgtcaaca ccaaaacttc ttcttttaga tgaaccatca
    15601 atgggacttg ccccaatctt catccaagag atttttgata tcattcaaga tattcagaag
    15661 caaggaacaa ccgtcctctt gattgaacaa aatgccaata aagcacttgc aatctctgac
    15721 cgaggatatg tattggaaac aggaaaaatc gtcctatcag ggtcaggaaa agaactcgct
    15781 tcatcagaag aagtcagaaa agcatatcta ggtggctaaa acaatccaga ggattgtttt
    15841 agtcggcaga tggagattac gaagtaatca tcaatatagt ccgggggacc tttttagtcg
    15901 gtagattgag attgcaaaca aatctgcatc tacattgaaa gcttaatttc taataattga
    15961 aaaaatcgaa tgaaaagttt cttaccttca ttcacagagc tcgattttag agctcttttt
    16021 gctagcttat tcatactttt ctaaattttg aaaaagaaat gtaagcgttt gatcgatttg
    16081 caaaaagatt gtataatagg gataagaata gaaaaggaga agtctcatgg cagttaaaga
    16141 ttttatgacc cgcaaggtag tttatattag tccagataca acagtatctc atgcagcaga
    16201 tttgatgaga gagcaaggtt tgcaccgtct gcctgttatc gaaaatgatc aattagttgg
    16261 tttggtgact gagggaacca ttgcacaagc aagtccatct aaagcaacaa gtctttctat
    16321 atatgagatg aattatcttc tgaataagac aaaagtaaaa gatgtcatga ttcgcgatgt
    16381 tgtcactgtc tcaggctatg ctagtctaga agatgcaact tatctgatgt tgaaaaataa
    16441 gattggtatt ctccctgtcg tagataacca tcaagtatac ggagttatta ctgaccgtga
    16501 tgttttccaa gcctttcttg aaattgcagg ttatggcgaa gaagggattc gtgtacgctt
    16561 tgttacagaa gatgaagttg gtgttcttgg aaaaattgtt tctttgattg tagaagaaaa
    16621 tttgaatatc tcccatacag tcaatattcc gcgtaaggat ggtaaggtga ttatcgaagt
    16681 gcaaatcgat gaatcaattg atttaccagc cttgaaagaa aaatttgaag caaatggtat
    16741 tcaagtggaa gaaatcgctc gcacttcagc aaaagtcttg taagaaggga agcccaaagg
    16801 cttctttttt catgaaaagg ggattagagc aaaagatgga aagaaatgat aaaatatgct
    16861 ataatgaaat aatgtaaaaa aggagtattt atggacattt cagtaattcg tcagaaaatt
    16921 gacgcaaatc gtgaaaaatt agcttctttc agggggtctc tttgacctcg aagggctaga
    16981 ggaagagatt gccatcttgg aaaacaagat gacagaacct gatttttgga acgataatat
    17041 tgcggcccaa aaaacgtcgc aagaattgaa tgaattaaaa aacacttaca ataccttcca
    17101 taagatggaa gagttgcagg atgaagtcga aattttattg gattttttgg ctgaagacga
    17161 gtcagtgcat gatgaactgg tagcgcagtt agccgaactt gataagataa tgaccagcta
    17221 cgagatgact ctactcttgt cagaacctta tgaccacaac aatgccatct tggaaatcca
    17281 tccaggttct ggtggtactg aggcgcagga ctggggtgat atgttgcttc gtatgtatac
    17341 tcgttatggt aatgctaaag gctttaaagt ggaagtgttg gattaccaag caggtgatga
    17401 ggctggtatt aagtcggtaa ctttatcatt tgaagggcct aatgcctatg gtctcctcaa
    17461 gtcagaaatg ggtgttcacc gcttagtgcg aatctcacca tttgactctg ccaaacgtcg
    17521 ccatacctct ttcacatctg tagaagtgat gccagaattg gatgatacta ttgaagtgga
    17581 aatccgtgaa gatgatatca agatggatac cttccgttca ggtggtgccg gtggacaaaa
    17641 cgtcaataag gtttcaacag gtgtacgttt aacctacatt ccaactggaa ttgttgtcca
    17701 atcaacagtg gatcgtaccc agtatggaaa tagagatcgc gccatgaaga tgttgcaggc
    17761 taagctctat caaatggagc aagagaagaa ggctgcggag gtagattctc tcaaaggtga
    17821 gaaaaaggag atcacttggg gaagccaaat ccgttcttat gtcttcacgc cttatactat
    17881 ggtaaaagat caccgaacta gctttgaggt tgctcaggta gataaggtta tggatgggga
    17941 cctagatggt tttatcgatg cttatctcaa gtggcgaatt agctaagata gaaaggaact
    18001 cacatgtcaa ttattgaaat gagagatgtc gttaaaaaat acgacaacgg aacgactgct
    18061 ctacgcggtg tttcggttag cgttcaaccg ggggaatttg cttacatcgt aggaccttca
    18121 ggagcaggga agtcaacttt tattcgttct ctgtatcgtg aagtaaaaat cgataaagga
    18181 agtctatcag ttgctggttt taatctggtt aagatcaaaa agaaagatgt cccgcttcta
    18241 cgtcgtagtg ttggggttgt cttccaagat tataaattgt taccaaagaa aactgtctat
    18301 gaaaatattg cttacgctat ggaagtaatc ggggaaaatc gccgtaatat caaaagacga
    18361 gtgatggaag ttttggactt ggttggattg aagcataagg ttcgttcttt cccaaatgaa
    18421 ctctcaggtg gggagcaaca gcggattgcg attgcgcgtg caattgtaaa taatcccaaa
    18481 gtattgatag ctgatgagcc aacaggaaat ctggatccgg ataattcatg ggaaattatg
    18541 aatctcttgg aacggattaa cctacaagga acaactattt tgatggcgac tcataatagc
    18601 cagattgtaa ataccttgcg ccaccgtgtc attgccattg aaaatggccg tgtcgttcgt
    18661 gacgaatcaa aaggagagta tggatacgat gattagtaga ttttttcgcc atttatttga
    18721 agccttaaaa agtttgaaac gaaatggttg gatgacagta gctgctgtca gttcagtcat
    18781 gattactttg accttggtgg caatatttgc atctgttatt ttcaatacag cgaaactagc
    18841 tacagatatt gaaaataatg tccgtgtagt agtttatatc cgaaaggatg tggaagataa
    18901 tagtcagaca attgaaaaag aaggtcaaac tgttacaaat aatgactacc acaaggtata
    18961 tgattctttg aagaacatgt ctacggttaa aagtgttacc ttttcaagta aagaagaaca
    19021 atatgaaaaa ttaaccgaga taatgggaga taactggaaa atctttgaag gagatgccaa
    19081 tcctctctat gatgcctata ttgtagaggc aaacgctcca aatgatgtaa aaactatagc
    19141 cgaagatgct aaaaaaattg aaggtgtctc tgaggttcaa gatggcggtg ccaatacaga
    19201 aagactcttc aagttagctt catttatccg tgtttgggga ctagggattg ctgctttgtt
    19261 aatttttatc gcagttttct tgatttcaaa taccattcgt attaccatta tttcccgcag
    19321 tcgcgaaatt caaatcatgc gcttggtcgg agctaaaaac agttatatcc gtggaccgtt
    19381 cttgttagaa ggagccttta tcggtttatt gggagctatc gcaccatctg ttttggtctt
    19441 tattgtttat caaattgttt accaatctgt caacaaatcg ttggtagggc aaaatctatc
    19501 catgattagt ccagatttat ttagtccgtt gatgattgcc ctactatttg tgattggggt
    19561 tttcattggt tcattgggat caggaatatc catgcgccga ttcttgaaga tttaggtaaa
    19621 atagctgctt ttatgaggag attgtaaaat ctcctttttt gctacaagag tttttgaaaa
    19681 gagatgcgca gaagaaaaga gcttccaaag aagtccccca gagaagactt cttgatggct
    19741 aatttgaact tgaaatttgg cgcactagaa attttttaga aaaaagtgta atttttttat
    19801 aaaagccttt acaaaaaaaa ttaaagtggt ataattaaat catagaaagt gcaaacgttt
    19861 tcgtaaaacg tttgcctaaa taaaaataaa ggagatttga atgatgaaag atacattcaa
    19921 aaatgtcttg tctttcgaat tttggcaaaa attcggtaag gctttgatgg tagttatcgc
    19981 ggttatgccg gctgctggtt tgatgatttc aatcggtaag tctatcgtga tgattaaccc
    20041 aacctttgca ccacttgtca tcacaggtgg aattcttgag caaatcggtt ggggggttat
    20101 cggtaacctt cacattttgt ttgccctagc cattggagga agctgggcta aagaacgtgc
    20161 tggtggtgct ttcgccgctg gtcttgcctt catcttgatt aaccgtatca ctggtacaat
    20221 ctttggtgta tcaggcgata tgttgaaaaa tccagatgct atggtaacta ctttctttgg
    20281 tggttcaatc aaagttgctg attactttat cagtgttctt gaagctccag ccttgaacat
    20341 gggggtattc gtagggatta tctcaggttt tgtaggggca actgcttaca acaaatacta
    20401 caacttccgt aaacttcctg atgcactttc attcttcaac gggaaacgtt tcgtaccatt
    20461 tgtagttatt cttcgttcag caatcgctgc aattctactt gctgctttct ggccagtagt
    20521 tcaaacaggt atcaataatt tcggtatctg gattgccaac tcacaagaaa ctgctccaat
    20581 tcttgcacca ttcttgtatg gtactttgga acgtttgctc ttgccatttg gtcttcacca
    20641 catgttgact atcccaatga actacacagc tcttggtggt acttatgaca ttttaactgg
    20701 tgcagctaaa ggtactcaag tattcggtca agacccacta tggcttgcat gggtaacaga
    20761 ccttgtaaac cttaaaggta ctgatgctag tcaataccaa cacttgttag atacagtaca
    20821 tccagctcgt ttcaaagttg gacaaatgat cggttcattc ggtatcttga tgggtgtgat
    20881 tgttgctatc taccgtaatg ttgatgctga caagaaacat aaatacaaag gtatgatgat
    20941 tgcaacagct cttgcaacat tcttgacagg ggttactgaa ccaatcgaat acatgttcat
    21001 gttcatcgca acacctatgt atcttgttta ctcacttgtt caaggtgctg ccttcgctat
    21061 ggctgacgtc gtaaacctac gtatgcactc attcggttca atcgagttct tgactcgtac
    21121 acctattgca atcagtgctg gtattggtat ggatatcgtt aacttcgttt gggtaactgt
    21181 tctctttgct gtaatcatgt actttatcgc aaacttcatg attcaaaaat tcaactacgc
    21241 aactccaggg cgcaacggaa actacgaaac tgctgaaggt tcagaagaaa ccagcagcga
    21301 agtgaaagtt gcagcaggct ctcaagctgt aaacattatc aaccttcttg gtggacgtgt
    21361 aaacatcgtt gatgttgatg catgtatgac tcgtcttcgt gtaactgtta aagatgcaga
    21421 taaagtagga aatgcagagc aatggaaagc agaaggagct atgggtcttg tgatgaaagg
    21481 acaaggggtt caagctatct acggtccaaa agctgacatt ttgaaatctg atatccaaga
    21541 tatccttgat tcaggtgaaa tcattcctga aactcttcca agccaaatga ctgaagtaca
    21601 acaaaacact gttcacttca aagatcttac tgaggaagtt tactcagtag cagacggtca
    21661 agttgttgct ttggaacaag taaaggatcc agtatttgct caaaaaatga tgggtgatgg
    21721 atttgcagta gaacctgcaa atggaaacat tgtatctcca gtttcaggta ctgtatcaag
    21781 catcttccca acaaaacatg cttttggtat tgtgacggaa gcaggtcttg aagtattggt
    21841 tcacattggt ttggacacag taagtcttga aggtaaacca tttacagttc atgttgctga
    21901 aggacaaaaa gttgcagcag gagatctcct tgtcacagct gacttgaatg ctatccgtgc
    21961 agcaggacgt gaaacttcaa cagtagttgt cttcacaaat ggtgatgcaa ttaaatcagt
    22021 taaattagaa aaaacaggtt ctcttgcagc taaaacagca gttgctaaag tagaattgta
    22081 atatacttga ggttggaagc tgtattccaa cctcttattt tgggagaaaa gaatgaaatt
    22141 tttaacactc aatactcata gttggatgga gaaagaagca gaggaaaaat tccagatttt
    22201 gcttgaagat attcttgaaa aggactatga tttgatttgt ttccaagaaa ttaatcagga
    22261 gatgacctcg tcagaggtgg aggttaatga cctttatcaa gctttgccag cagctgagcc
    22321 tattcaccaa gatcactatg ttagactctt ggttgaaaag ttatctgagc aagggaaaaa
    22381 ttactactgg acctgggcct ataaccatat cggctatgac cgctaccacg aaggtgtggc
    22441 tatcttgtct aaaacaccta ttgaagccag agaaattttg gtttcagatg tggatgatcc
    22501 aacagactat catactcgcc gtgttgccct agctgaaact gtagtcgatg gcaaggagct
    22561 agcagttgcc agtgttcatc tctcttggtg ggataaaggt tttcaagaag aatgggcacg
    22621 atttgaggtt gtcttgaaaa aattgaacaa gccactttta ctagctggag attttaacaa
    22681 cccagcaggt caggaaggtt accaagctat tttagctagt ccattaggct tacaagacgc
    22741 atttgaagtt gctcaagaga aaagtggtag ctatactgtt ccgcctgaaa ttgatggctg
    22801 gaaagggaac actgaacccc ttcgaatcga ttatgtcttt actaccaaag agttagcggt
    22861 ggaaaattta catgtcgtat ttgatggtaa caagagtcca caagtgagtg atcactatgg
    22921 cttgaatgct atgttaaact ggaaataata actgaaaaga ggttggaact ataaaattcc
    22981 agccttttct tactagagaa gctactggaa atagcctaaa taagtgagac tactgtaatg
    23041 gaataaaata tggtataatt gataagatag atagaatcga ggatgttatg tcatttacga
    23101 aatttcaatt taaaaactat attagagaag ccttggagga gttaaaattt acaactccaa
    23161 cagaggtgca agacaagttg attcctattg ttttggcagg tcgtgaccta gtaggagaat
    23221 caaaaacagg ttcaggtaag actcatactt tcttgttacc gattttccag caattagatg
    23281 aagctagcga tagtgtacaa gcagtgatta ctgcaccgag tcgtgagttg gctactcaaa
    23341 tttaccaagt agcgcgtcag atttcagctc actcagatgt cgaagttcgt gtggttaatt
    23401 atgtgggtgg tacggataag gctcgccaga ttgagaaatt ggaaagcaat cagcctcata
    23461 ttgttattgg aacaccaggc cgtatctacg acttggttaa atctggtgat ttagctattc
    23521 ataaagccaa gacatttgtt gttgatgaag cagatatgac cttggatatg ggattcttgg
    23581 aaactgttga taagattgct ggcagtcttc caaaagactt gcaattcatg gtcttctcag
    23641 cgactatccc acaaaaactg cagccattct tgaaaaaata cttatcaaat cctgttatgg
    23701 agaaaattaa gaccaaaacg gttatttctg acaccattga taattggttg atttcgacca
    23761 agggacatga taagaatgct caaatttacc agttgactca gttgatgcag ccgtatttgg
    23821 caatgatttt tgttaacact aaaacgcgtg ctgatgaatt gcattcatat ctgactgctc
    23881 aaggcttgaa ggttgcaaaa atccatggcg atattgcccc tcgtgaacgc aagcgaatca
    23941 tgaatcaggt gcaaaatctg gattttgagt atattgtcgc aacagatttg gcagcacgtg
    24001 ggattgacat tgaaggtgtc agccatgtca tcaatgatgc cattccgcaa gacttatctt
    24061 tttttgttca ccgtgttggt cgtactggac gaaatggcct accaggtaca gctattaccc
    24121 tttatcagcc aagtgatgac tcggatatcc gtgagttgga gaaattggga atcaagttta
    24181 gtcctaagat ggtcaaagac ggggaatttc aagataccta tgaccgtgat cgtcgtgcca
    24241 accgtgagaa aaaacaagat aaacttgata tcgaaatgat tggtttggtt aaaaagaaaa
    24301 agaaaaaagt caaaccgggt tataagaaga aaattcaatg ggcggttgat gaaaagcgcc
    24361 gtaaaaccaa gcgtgctgaa aatcgcgctc gcggtcgtgc agagcgtaaa gctaaacgcc
    24421 aaacatttta atagaaattg ttggagtact gagctccaac ttttttattt atgagaacga
    24481 actatctaaa tcgaaacact acattaaaga ctgcaaattg cgattaaaaa tggtataatg
    24541 ataaagttat atagtcccga taagatggta ggtatttatt acgaagagtt ttcctatcag
    24601 tactttgtaa ctctataaca atatttttta aggggggaca tttttatgtc agaacgtaaa
    24661 ttattcacgt ctgaatctgt atctgagggg catccggata agattgcaga ccaaatttca
    24721 gatgcgattt tggatgctat tttagcaaag gatccagagg cgcacgttgc tgctgaaaca
    24781 gctgtatata ctggttctgt ccacgttttt ggtgaaattt ctacaaatgc ctatgtggat
    24841 attaaccgtg tggttcgtga taccattgca gagattggtt ataccaatac agaatatgga
    24901 ttttctgctg agacggtggg agtacaccca tctttggtgg aacaatctcc tgacatcgct
    24961 caaggtgtta acgaagcctt ggaggttcgt ggaaatgctg atcaagatcc actggacttg
    25021 attggagcag gtgaccaagg gctcatgttt ggatttgcag tagatgaaac agaagagctc
    25081 atgccattgc caattgcact cagtcataaa ttggttcgtc gtctggcaga acttcgtaag
    25141 tctggagaaa ttagctatct ccgtccagat gcaaaatcac aagttacagt tgagtacgat
    25201 gaaaatgacc gtccggtacg tgtagataca gtcgttattt ctactcagca tgatccagag
    25261 gccactaatg aacaaatcca tcaagatgtg attgacaagg tcatcaaaga agttattcca
    25321 tcttcttatc ttgatgataa gacaaaattc tttatcaatc cgacaggtcg ttttgtaatc
    25381 ggtggtcctc aaggggactc aggtttgact ggtcgtaaga ttattgtaga tacttatggt
    25441 ggctactctc gtcatggtgg tggtgccttc tctggtaaag atgcgactaa ggtggatcgt
    25501 tcagcttctt atgcggctcg ctatattgcc aagaatatcg ttgcagcagg ccttgctaag
    25561 aaggcagaag tgcagttggc ctatgctatc ggtgttgcgc aacctgtttc tgttcgtatc
    25621 gatactttcg gtacaggaac agtagctgaa agtcaacttg aaaaagcggc tcgtcaaatc
    25681 tttgaccttc gccctgcagg gattatccaa atgctggacc ttaagcgtcc aatttaccgt
    25741 caaacatcgg cttacggtca catgggacgt acagatattg atcttccatg ggaacgtttg
    25801 gataaggtag atgctttgaa agaagcagta aaataagatt ttaagagggg aacgtcctct
    25861 cttttttata gtttttaact atactgggat actattctga aaatccattt tgcgaaagta
    25921 gagatttaca tgtatagtag attgaaacta gaatagtaca cctcaacttc taaaacattg
    25981 ttagaaatcg atttgactgt cctgatcgat ttctcctgtt cttgtttcat tttactatat
    26041 ttctctaaaa atgataaagg ttgagatttc tcctcgtaat agataatcat ggggatattt
    26101 caatccaaag ttttattcgt tatcacttga ctattgcaag gttttctaga gcaacagagt
    26161 catggaatgg actcatggtt gagatttctc cttgttgctt ggacttcatt caaaagtctg
    26221 ttacccaagc cttgttcaaa cttctaatac actagctgtt tccatagcat gacttctgta
    26281 ctagactttc ttttccgaat aaatagatag aaccacagaa tctagtaaac ctagaattaa
    26341 aattatggta taatattagc aataaaagaa atctggagga ttagaatcat ggtatcaacg
    26401 aaaacacaaa ttgctggttt tgagtttgac aattgcttga tgaatgcagc aggtgtggct
    26461 tgtatgacga tagaggagtt agaagaggtc aaaaactcag cggcaggaac ctttgttact
    26521 aagacagcga ccttggactt ccgtcagggg aatcctgagc cacgctacca agatgttcca
    26581 cttggttcca tcaactctat gggcttgcca aataatggct tagactatta tttggattat
    26641 cttttagatt tgcaggaaaa agagtcgaac cgaactttct tcttatctct ggtcggcatg
    26701 tctccagagg aaacccatac tattttgaaa aaagtccaag agagtgattt tcgtggtctg
    26761 actgagctaa atctttcctg tccaaatgtt ccaggtaaac ctcagattgc ctatgatttt
    26821 gagacaacag accggatttt ggcagaagtg tttgcttact tcaccaaacc tcttggaatt
    26881 aaattgccac cttattttga tattgttcac tttgatcaag cggcagctat tttcaacaaa
    26941 tatccgctca agtttgtcaa ctgcgttaac tctatcggaa acggcctcta tatagaagac
    27001 gaatctgtcg ttattcggcc taagaatggt tttggtggaa ttggtggaga atacatcaaa
    27061 ccgactgctc tagccaatgt tcacgccttt tatcaacgtt taaatcctca aatccaaatt
    27121 atcggaacag gtggcgttct gactggtcga gatgcctttg aacacatcct ctgtggagca
    27181 agtatggtgc aggtgggaac gacccttcac aaagaaggcg tcagtgcttt tgaccgcatt
    27241 accaatgaac tgaaagcaat catggtggaa aaaggctacg agagcttaga agatttccgt
    27301 gggaaattgc gctatattga ctaaattaaa tcgaaaaatc tgaagaaagg agagacgatg
    27361 ctagccattg aagaaagtca gaagttgact ttatcaaatt taccgagcct gagcctattt
    27421 acagggacag atcagggtca gtttgaagtg atgaagagtc aagtgttgaa acagattggg
    27481 tatgattctg ctgacctcaa ctttgcctac tttgatatga aagaagtagt ttacaaggat
    27541 gtggaactgg agttggtcag ccttcctttc tttgcggatg agaaaatcgt gatattagat
    27601 tattttatgg atatcacgac tgctaagaaa cgctttttga cagatgatga gcttaagtca
    27661 tttgaggaat accttgacaa tccttctcca acaaccaagt tgataatctt tgcagaagga
    27721 aagctggata gcaaaagacg gttagtcaaa ttacttaagc gtgatgccaa ggtcttcgat
    27781 gcagtagaag taaaagaaca agaattgcgc cagtacttcc aaaagtggag tcagaaacaa
    27841 ggtctgcagt ttaccaatca ttcttttgaa aatctcctca tcaagtcggg gtttcaattt
    27901 agcgaaatcc agaaaaatct tctcttttta cagtcctata aggtggattc tgttatcgag
    27961 gaagaggata ttgttaacgc aattcccaag accttgcagg acaatatttt tgatttaact
    28021 cagtttattc tgactaaaaa gatggatcag gcgcgcgatt tggtgagaga cttgaccttg
    28081 caaggggaag atgaaatcaa actgattgca gtcatgctgg gacaatttcg gacttttact
    28141 caggtgaaga ttttggcgga gtctggccaa acagaatcgc agattgcaag tagtttaggt
    28201 agttatctgg gacgtaaccc aaatccttat caaatcaagt ttgcattaag agattcgaga
    28261 ggactttctt tgagctttct gaagcaagct atttcctatt tgattgagac agactatcag
    28321 attaagacag gtctttatga aaaaggtttc ctttttgaaa aggcactctt acagattgct
    28381 agtcaggtca attgacattt gttgaaacta caacccgcgt caggattatc ttgtcgtggg
    28441 tttcttgctg atttgcttat tttattagaa agtatgtcag tgatttcctt atctataagc
    28501 aaaaaacttg aaaaaagtga atgtttgcgt tatcattttc atatagaaag aaaaagaggt
    28561 attacagatg gctattatct taccagaact tccatatgca tacgacgctt tggaaccata
    28621 catcgatgcg gaaacaatgc atttgcacca tgacaaacac catcaaactt atgtcaacaa
    28681 tgccaatgca gctttagaaa aacaccctga aatcggtgaa gaccttgaag ccttgcttgc
    28741 tgatgtagaa tctatcccag ctgatatccg tcaagcactt atcaacaatg gtggcggaca
    28801 cttgaaccac gctcttttct gggaattgat gactcccgag aaaacagctc cttcagcaga
    28861 actggcagca gcaatcgatg caacatttgg ttcatttgaa gaattccaag cagccttcac
    28921 tgcagcagca acaactcgtt ttggttcagg ttgggcatgg ttggttgtca acaaagaagg
    28981 gaaacttgaa gtgacttcaa cagcaaacca agacacacca atctcagaag gtaaaaaacc
    29041 aatcttgggc ttggacgttt gggaacatgc ttactacgtg aaataccgca acgtgcgtcc
    29101 tgactacatc aaagctttct tttcagtaat caactggaat aaagtagatg aattgtacgc
    29161 agctgctaaa taatgatagt tggagggaag aattgttctt ctctttttta ggttataaga
    29221 ttctggtctg acaaaatcgt tagacttttt tcatttttta tgagaaacgt gctaactgct
    29281 agaaattatg gtagaataaa gtattaagta atcgtggaaa aaggatatct atgcgaaaag
    29341 aaattgcacc tgaattatac aactataaca agtttcctgg tccggagttc catttacacg
    29401 gggacaaggt cgaaacggaa gggatagctt tttccttggt tgaaaatatc aaggatgcct
    29461 ttgatgtgac gacttttaat cagcgttttt cagaagtctt aaccaagttt gattatatcg
    29521 tgggggactg gagcaacgaa cagcttcgcc tacgaggttt ttacaaggat gaccgaacag
    29581 aagaaaaact tgaaaaaatc agtcgtttac aagactacct tttagagtat tgtagttatg
    29641 gttgtgccta ttttgtctta gaaaatgaag cccctaagcg agcatcattt gacaagaaaa
    29701 tgcgtaagac ggaagaagaa acaccttcta gaaaaggaaa gaaaccgact caaactaaac
    29761 gaaagtcgaa tgcagataag aaaaatagac gtcgtcagaa agaccagcat tctcagaaag
    29821 aggacaagga acaacgtcat tttgtcattc gtcagaagtg agtagagaat aaggagaaga
    29881 aatgaaaccc tcaattcata gtttggtaca tcaaaccatg caggaatggg tattggagca
    29941 gggagaaaag aaattccgtg cagatcaaat ctgggaatgg ctctaccgta aacgtgtgca
    30001 gtcatttgaa gaaatgacca acctttccaa ggatttgatt gctaagctca atgaccagtt
    30061 tgtggtcaat cccttgaaac aacgtattgt gcaagagtct gctgatggta cagtcaaata
    30121 tctctttgaa ttgcctgacg gtatgttaat tgagactgta ctcatgcgtc aacactatgg
    30181 tttatctgtc tgtgtgacca ctcaggtcgg ctgtaatatc ggttgtacct tctgcgcctc
    30241 tggtttgatt aagaagcaac gtgacctcaa taacggtgaa atcgtagcgc aaatcatgct
    30301 ggttcagaaa tactttgatg agcgtggtca ggatgaacgc gtcagccata tcgttgtcat
    30361 gggaatcggt gagccctttg ataattacaa caatgtcttg aatttctttc gtactatcaa
    30421 tgatgataaa ggaatggcta tcggtgctcg tcacatcacg gtttcaatct caggtttggc
    30481 tcataaaatt cgtgactttg ctgatgaagg ggttcaggtc aatcttgccg tgtctcttca
    30541 cgcacccaac aatgaacttc gttcaagtat catgaagatt aaccgtgcct ttccgattga
    30601 aaaactcttt gctgctattg aatactacat tgagacaaca aaccgtcgtg taacctttga
    30661 atatatcatg cttaatgaag tcaacgacgg tgtagaacaa gccttggaat tggctgaatt
    30721 gctcaagaac atcaagaaat tgtcttatgt aaacttgatt ccttataacc cagttagtga
    30781 gcatgaccaa tatagccgta gtcccaaaga gcgcgtgttg gctttctatg atacgcttaa
    30841 gaaaaaaggg gtcaactgtg tggttcgtca agagcatggt acagatattg acgcagcttg
    30901 tggacaattg cgctctaaca caatgaaacg tgaccgccag aaggcagtcg cagcggtaaa
    30961 tccttaatga tgaagaagac ttataatcat attttggtct ggggagtcat tttctatagc
    31021 atttgcattg tctgtttttg ctttactcct caagaacaat ctaccgtggg agtgggaact
    31081 ccagatattc agcatcttgg acgcctggtt tttcttttga ctcctttcaa ttctctctgg
    31141 aaactgggcg aagtgagtga cattggacaa ttatgttgga tttttttaca aaatatcctc
    31201 aatgtcttct tgttttttcc tctgattttc caactccttt atctatttcc aaatttgcgg
    31261 aaaacaaaaa aggtccttct ttttagtttt cttgtgagtc ttggaatcga gtgtacgcaa
    31321 ttaatcttgg actttttctt tgatttcaat cgcgtctttg agattgatga tttgtggacc
    31381 aacactttgg gtggctatct ggcttggctc ctttataaac gattacataa aaacaagata
    31441 aggaattaaa atgagtattt tagaagttaa aaatctgagt cacggttttg gtgaccgtgc
    31501 aatttttgaa gatgtgtcct tccgtctcct caagggagaa catatcggcc tggtcggtgc
    31561 caatggtgaa ggaaaatcaa cctttatgag tatcgtgact ggtaaaatgc tgccagatga
    31621 aggaaaggtt gagtggtcca aatatgtgac ggctggttac ttggatcagc actctgtcct
    31681 tgctgaaaga cagtcggtgc gtgatgttct ccgtacggct tttgatgagc ttttcaaagc
    31741 tgaagctcgt atcaatgacc tctatatgaa aatggctgaa gacggcgcgg atgttgatgc
    31801 tctcatggaa gaagtaggag aacttcaaga ccgtctggag agtcgtgatt tctatacctt
    31861 ggatgctaag attgacgaag tagcgcgtgc tcttggtgtt atggactttg gcatggatac
    31921 ggatgtaact tctttgtcag gtgggcaaag aaccaaggtg cttttggcaa aacttctcct
    31981 tgaaaagcct gatattttgc tgttggacga gccgaccaac tacttggatg ctgagcatat
    32041 tgattggctc aagcgctatc tccaaaacta tgagaatgcc tttgttctca tttcgcacga
    32101 tattccattc ctcaatgacg ttatcaatat tgtctatcat gtggaaaatc aacagctgac
    32161 gcgttactct ggtgactact accagttcca agaagtttat gctatgaaga aatctcagct
    32221 agaggcagcc tacgaacgcc agcagaaaga gattgcagac ctcaaggact ttgtggctcg
    32281 taataaagcc cgtgttgcaa ctcgtaatat ggctatgtct cgtcaaaaga aattggataa
    32341 gatggatatt atcgaactcc aaagtgagaa accaaaacca tcctttgatt tcaaaccagc
    32401 tcgtacacca gggcgcttta tcttccaagc caagaacttg caaattggtt acgaccgtcc
    32461 tcttactaag cctttaaatc ttaccttcga acgcaatcaa aaggttgcga ttattggtgc
    32521 taatggtatt ggaaaaacaa ctctcttgaa gagtctcttg ggcattatct cgccaatcgc
    32581 tggggaagtg gagcgtggag attatttaga acttggttat tttgagcagg aagtagaagg
    32641 cggtaatcgc caaactcctc ttgaagctgt ctggaatgcc tttcctgccc ttaatcaagc
    32701 agaagtccgt gcagcccttg cccgttgtgg tttgacaacc aaacatattg aaagccagat
    32761 tcaagtatta tcagggggag agcaagccaa ggttcgtttc tgtctcttga tgaatcgtga
    32821 aaacaacgtt ttagtgctgg acgagccgac caaccatttg gatgtggatg caaaggatga
    32881 gctcaaacgc gctctcaaag aatatagggg atctatcctt atggtctgcc acgagccaga
    32941 cttttatgaa ggctggatag accaaatatg ggattttaat aatttaactt aagaatgaga
    33001 gtaaaagaaa tacagtaccg aatgtggata ctgtattttt ttggttttta agatttaaaa
    33061 tcgtagtctt tcaccacttc gatgctgtcg attgtgatag cagtagttgg cttgtctttt
    33121 tcatcttttt cggccttagc aatcttatcc acaacatcca taccgtcaat cacttgacca
    33181 aagactgggt gtttgccatc tagactaggg tttccacctt ctttgtaggc ttcgataatt
    33241 ttctgtggat acttgcttgt agggagttta gaagaggtat ctgtagagtt ttggttgatg
    33301 aagaactggc tgccattggt gtttggttga ccagtattag ccatagcaag agcaccacgg
    33361 atgttataca aataaggagt aatctcgttc ttgaaaccag ttcctttgtc tttagtctta
    33421 tccttgtcat gccagatgga ctgaccacct gtaccgtccc cttttggatc tccagtttgg
    33481 accataaagc catcgatgac acggtggaag gtaataccgt tatagtagcc ttctttggcg
    33541 tgagtgagga aattttcaac cgctagagga gcgagtttag ggaagagttt aatgcgaata
    33601 tcaccttggc ttgtgtggag aatcacttcg gcttcatctt cagcaacttc cttagatagt
    33661 tgtgggaaat tggcgttttc gtttgttaaa gcatcgttta actccttggc agattgggca
    33721 gctactttgg aactttcttc agcagcaaga ctggaatcaa cataatcatc accacgcaga
    33781 ctgcgttgga cgctgctaca cccagctagg gctacagtag acagtaaaag aagggttgct
    33841 agttttttca ttctgatcct ctcaaaaatt catttataac cattgtaccc taaaaggaag
    33901 tggatttcaa atattagtag tttacagaac tggcttagat aatagttggg atttcatcat
    33961 catcttgaaa atcttcaact tcttccccag taaatggttt ctgaaaagaa cttagtataa
    34021 aacgggcaat tggttcaaca attaggaaat ttagaggaag agctgcaata aagtttacga
    34081 accaggcgct tagataggta ttgggtgtga tagcaatgcc attgtaaaaa agtccataga
    34141 aggacatgaa agtgaccagc taattcttct tctgaaaaat gctcatgaag ggcttgatcc
    34201 aggtcgcaag atttcatttt agaaagtcca gtaactccct tcttcttcag gatattcttt
    34261 ttcatggtat tatttagatg gtcaagtgag tcaaaagaag tttcgacaag agcataccct
    34321 ttttctacca gagtttttag atgtttaggg gcatcaatac cataagtgta ctcaaagtat
    34381 tttggaaacc agatcttaag aaagctcgta aagctagcca gttctctaaa cgctaagaac
    34441 tagctacaac acagcattta caacacttca tggttcacac catggggtgt tttttaatga
    34501 ttttaagcaa aattcacact taattgaaag ttatttaaat tagatgaaaa tttatttttt
    34561 gaaaaagtgg gaaaacattg attctaagcg aattttgaat catattcgaa tgggagtttt
    34621 taaaatcatg tttcagtggt aaaagtacca aactggattc tccatagtaa aatgatatct
    34681 cctgctagtg aatcatctac taataagtcc atatttcgct tgggaacggg cttgggactt
    34741 agaagccaag tatccaactc tcgttcaggc gatatgaagg gtttgacttg gtgttcttta
    34801 taaaattttt ctaaaatagt attcacggat agcatctttc aaacatttat ttcctattct
    34861 aacaaatttc taaatacttt aagaaattta tagtctgtca agtttttttc ttgacacatg
    34921 ccagaaatct gctataatag aacatgtgct aaatagctca gctatttcac cgaaataaaa
    34981 aattaaagaa atgagacttt aaaaatggca gttaaaatcc gtttgactcg tatgggttct
    35041 aagaaaaaac ctttctaccg tatcaacgta gcagattcac gttcaccacg tgacggacgt
    35101 ttcatcgaaa cagttggaac ttacaaccca cttgttgctg aaaaccaagt aactttgaaa
    35161 gaagaccgcg ttcttgcatg gttggctaat ggagctcaac cttcagacac agtacgtaac
    35221 atcctttcaa aagaaggcgt attgaaaaaa ttccacgatt ctaaattttc aaaataagtt
    35281 taaagtaggt tgacagatgg atacgattga aaatctcatt attgcgattg tgaaaccctt
    35341 aatttcacaa ccagatgcct taactatcaa gattgaggat acaccagaat ttttggaata
    35401 tcatttgaat cttgatcaaa gcgatgtggg tcgtgtaatc ggtcgtaagg gtcgcactat
    35461 ttctgcgata agaacgattg tctactctgt cccaactgaa tacaaaaaag taagaatcgt
    35521 tattgacgaa aaataagaag ggcgggacgg atgtttcgct tttttgtgtg gaatagaaga
    35581 tatagtaaaa tgaaacaaga acaggacaaa tcgatcagga cagtcaaatc gatttctaac
    35641 aatattttag aagtaaaggt gtactattct agtttcaata tactataaaa gattgaacgt
    35701 ttaggcaatt ccaagactac ttaatcgaac attaacagca gtctcggact gatgaaggtc
    35761 tctttatgaa gttagtggag gaagtcggaa aagtagccga ggttttgaat aggcgctctg
    35821 gtcgaaaaga agggatagga ttcgaacgag gaacttgcca gagaactggc tgatatcatt
    35881 tactacaccg tcgcaatcgt agctattaat catattgacc ttaccaagac tgtctttgag
    35941 aaagacaaaa ccgcagccgt taattaccaa cataaacata atttggaagg attattgaag
    36001 ggaaagtaga gatgttgtcc ccttcctcca acttgagaaa atccttggaa cgtgataaaa
    36061 taaaggataa tgatttttaa taattcatta tatagaatga gtggatttct ttatgaagag
    36121 gtttgaagta tctacagaaa ttggtagcct ctctgttact tatcaaaagc aaaagaaagt
    36181 gctagtttgt ctaaacggag caggtttgct accaagttat gaaaattttt cacttatact
    36241 tgaaaaacct cctcctacaa ttggttattt gacaattgat tttccgaaca caggtaggag
    36301 tccgattcat gaccaagctg gaaaaaatct agataatctt gcagatgcgg tttatgaagt
    36361 acttgaagaa ttaggaattt ctgaatatat actttgtgca catagttgga gtggaatttt
    36421 agcttgcaaa ttactcgaga aaccaattaa gcgtcagact ttagtagcaa ttgaaccgac
    36481 aactaaaaaa gtcatgtttg ctgatttttc agaaaatcct tatccaaaaa tggaagagca
    36541 gatgaggctg attgacgagt gtggtcctga actttatttt aagaacttaa ctcaagcaac
    36601 atttagtcct gaaacgaata aaaaaatctg ggaattaatg caagaaaaag gcttagagtt
    36661 ggaaaatcaa gatccagaat ttcagatatc tggagagatt actgaggaag attttgagaa
    36721 tttgtcgata gaatctcatg tccctgtttt tattttttgt cagacttata gagaaaaaga
    36781 gtacagagaa tcagaatatt ggacttccaa tactaaactc attttaggag ggaatcacca
    36841 ttatttacaa tggtcagaat cggaaaaaat tgcggctatt attcgagaat tgtcagaata
    36901 agatggaaaa aaggagatta caggagacaa gatgaactac tttaatgttg ggaaaatcgt
    36961 taatacgcag ggattacagg gtgagatgcg agtcttgtct gtgacggatt ttgcagaaga
    37021 acggtttaaa aaaggagctg agctggcttt gtttgatgaa aaagatcagt ttgtccaaac
    37081 agtgaccatc gctagccacc gtaaacagaa gaactttgac attattaaat tcaaagatat
    37141 gtaccatatc aatactatcg aaaagtacaa gggatacagt ctcaaggtcg ctgaggaaga
    37201 tttgaatgac ctagacgatg gtgaatttta ctatcacgag attatcggtt tggaagtcta
    37261 tgagggtgat agcttggttg gaaccatcaa ggaaatcctg caaccaggtg ctaatgatgt
    37321 ctgggtggtc aaacgaaaag gcaaacgtga tttgctttta ccttatatcc caccagtggt
    37381 tctcaatgtt gatattccaa ataaacgggt cgatgtggaa atcttagaag ggttagacga
    37441 tgaagattga tattttaacc ctctttccag agatgttttc tccactggag cactcaatcg
    37501 ttggaaaggc tcgagaaaaa gggctcttgg atatccagta tcataatttt cgagaaaatg
    37561 ctgaaaaggc ccgtcatgta gatgatgatc cctacggagg cggtcagggc atgttgctca
    37621 gagcacaacc tattttcgat tcctttgatg ctattgaaaa gaaaaatccg cgcgttattc
    37681 tccttgatcc tgctggaaag cagtttgatc aggcttatgc tgaagatttg gctcaagagg
    37741 aagagctaat ctttatctgt gggcactatg agggttatga tgagcgcatt aagaccttgg
    37801 taacagatga gatttcccta ggcgactatg tcctcactgg tggagaattg gcagctatga
    37861 ccatgattga tgctacagtt cgcctgattc cagaagtgat tggcaaggag tctagccacc
    37921 aagatgatag tttttcttca ggtcttttag aatatcctca gtacacacgt ccctatgatt
    37981 atcgaggcat ggtcgtgcca gatgtattga tgagtggcca ccatgaaaag attcgtcagt
    38041 ggcgattgta cgagagttta aagaaaacct acgagcgcag accggattta cttgaacatt
    38101 atcaactgac agtagaagaa gaaaaaatgc tggcagaaat caaagaaaac aaagaataaa
    38161 ggagaaacct atgcaagtaa tcaaacgtaa tggcgaaatt gctgaattta atccagataa
    38221 gatttaccaa gccatcttga aggcagccca aactgtttat gttttgacag atgatttgcg
    38281 tcaaaatctt gctcaagtca ctaagaaggt agttttggat ttacaagaag ccaaggtgga
    38341 acgtgcgact atcagtatga ttcaatctat ggttgaacat cgtttattgg gcgcaggtta
    38401 cattaccatt gcagaacact acatttccta tcgtttacaa cgtgacttgg aaagaagtgg
    38461 ttatggagat catatcgcag ttcatttaca ttttgaacaa attcgctaag aaaaaagagt
    38521 gggatgaagc aactacatct cactctttct taaatctatt tttttgggct gtttggacag
    38581 ttgaggggac aaatcgcagg cgttgaatat cgcttatacg gataatccgg ctcacatttt
    38641 tggcaaaatt tttcacgata atttgctggc cttgctgatc atatttgatg atatcacctg
    38701 taaagcttgt ctcagacaag ataaggtgaa cagcggtttg tttctggata gcctcttcaa
    38761 tagtggctgt aagggagttg gtttcagtct ggtcaggttg gtcatgtcca tttaaaaaat
    38821 catgtatttt atctagaact tgctggaatt tatgtctcat attggcctcc cttcttctat
    38881 atcaatatta tataaaattt tcctaaaatc tacaagattt tacgaataaa ctaatgaaac
    38941 taaaaaagga gaagaaaatg gcagaattta catttgaaat cgaagagcac ttgttgactc
    39001 tttctgaaaa cgaaaaaggt tggaccaagg aaattaaccg tgtgagcttt aatggtgccc
    39061 ctgcaaagtt tgatattcgt gcttggagtc cagaccatac taaaatgggc aaagggatta
    39121 ctctctcaaa tgaagaattt caaacgatgg tggatgcctt taaaggcaac taatactctt
    39181 cgaaaatctc ttcaaaccgc atcagctttg ccttgccgta tgtatggtta ctgacttcgt
    39241 cagtttcatc cacaacctca aaacaatgtt ttgagctgac ttcgtcagtt tcatccacaa
    39301 cctcaaaaca gtgttttgag cagcctgcgg ctagcttcct agtttgctct ttgattttca
    39361 ttgagtataa gttttcaaaa taaaaaaaaa ggatattgag tcatgataac tcgatatcct
    39421 tttttagtta gtaaagtaag cctgattttt aaggcgttga agtaggggac ggctaataaa
    39481 actaatagcc agaagtttgc caaggtctgg aatgataaag ggaagaaccc ccacaacaag
    39541 agctttttca aatgccattc cagctaggaa atgcaagctg agaatcccgc cgacaaagac
    39601 aagggcatca cccaagaggt ttgcaagaaa aatcttaaca acaccactct tgctgttggt
    39661 tagagaggaa gtaagtccag agtaaacgag ataaaaccaa agatagcctg cagtagggcc
    39721 aactaaagcc tgaaaaccag ctccgcctcc tgcaaagaca ggaagaccga tagcacctag
    39781 aagaagatag agtccagcag aaagtacagc ctctctcggt ctaaagacag tagcaatcaa
    39841 gccgattgca aagttttgca gagtgaaggg aacaggtcca attggaagac tgatttgtgc
    39901 caaaacagca atgagagcag ccccaatagc agggatagca taaacgtgag cttttttcaa
    39961 aatagttaac ctcttttcta atgtatagta actattatac tcatatagga gtcgatgtca
    40021 actatttttt aaaaacaagg taacaaaaac tgtaacagcc gattttcgtg actagtcaag
    40081 ttggataatt tttttgttga ttcgaaaatc attaaaaaag aagcagacat atttatatca
    40141 gcttcttttg tttcgattaa cgcatggtta caaattcttc agatgaagtt gggtgaatcg
    40201 ctacagttgc gtcaaagtca gccttggttg ctcccatttt gatagcaaca gcaaatcctt
    40261 gaatcatttc atcaacgccg tagccaattc cgtgaagtcc gacaactttt tcttctgaag
    40321 cagctgttat gagtttgaaa cgggattctt gacggttgcg agtgcaagca gagtacatag
    40381 atgcaaagct tgatttataa accttgattt ggtcttgacc gtattcttta atagcttgct
    40441 cttctgtcaa accaacagtt ccgatagcag ggtgtgaaaa gacaacagtt ggaatagttg
    40501 agtaatccat ttttgcagta gtttttccgt taaagagacg ttcagatagg gtacgtccgg
    40561 ccttgattgc aactggagtc agttctttct cgcccgttac atcacctaga gcatagattc
    40621 cctcaacgac ggtattttgg tattcatcta cttggataaa gccacgttca ttcagagtca
    40681 ctccagcttt ttcaagttgc aagcccttaa cgtttggacg gcggcctgta gcccagataa
    40741 cttggctagc tgtgtgacta gtaccatctt cgaaatgaat ggtaatgccg tcagtagttt
    40801 tttctaactt gacagggact ttgtgagtgt gaagtggtaa gtttgttctt tccatttcct
    40861 tgaccaaacc ttcaacgatg taggaatcaa aaccacgtaa aggacgatcg cggcgaacaa
    40921 agagatctgt cttgacacca aaagtgtgga gtacgccagc taattcaacg gcgatataac
    40981 cagcgcctag aatggcaact gactctggaa gttcttccca ggcaaataca ttatcagaag
    41041 agccacctag ctcagcacca ggaatattag gaatacttgg atgggcacct gtagcaatca
    41101 cgatatgttt agcacgaatc agttcaccat ttacgcttac agtatgagaa tctacaaatt
    41161 cagcatgacc ttcaatcaag tctacaccgt tgcgtttaaa actgccatca taagaagaac
    41221 gagcgcgatc aatgtaggat tcacgattgc gacgtagggt tgcaaagtca aagttaagat
    41281 cagtagtctt aaagccgtag tcttctccaa attgatggaa agtctcagcg atttgtgccc
    41341 cgtaccacat gattttttta ggaacacaac cgacgttgac acaggttcca cctaatttct
    41401 tttcctcaat aacggctgct ttggctccat gttcaccagc acggttcatg gtagcgattc
    41461 ctccgctacc tccaccgata gcaatgatat catattctct cattgaaaac tcctttgtaa
    41521 tcttttaaat agtagagcgt cttttttgat agttatattt tattcttttt ttgatttgat
    41581 tgcaaaaatc tgctacgcac aaaaaattgc aaaaaagctt gccaaataag tcaagatagt
    41641 gtattatata tctagtacaa tagaaaagta aaattccgaa cgattgttta atcctgaact
    41701 gccattattt ccatctgaac tgcattgttt gctggaggga aatttggtat aatagcaaag
    41761 tattgtaaaa aagaaaagga gtttcattat gaagaaaaag aatggtaaag ctaaaaagtg
    41821 gcaactgtat gcagcaatcg gtgctgcgag tgtagttgta ttgggtgccg gggggatttt
    41881 actctttaga caaccttctc agactgctct aaaagatgag cctactcatc ttgttgttgc
    41941 caaggaagga agcgtggcat cctctgtttt attgtcaggg acagtaacag caaaaaatga
    42001 acaatatgtt tattttgatg ctagtaaggg tgatttagat gaaatccttg tttctgtggg
    42061 cgataaggtc agcgaagggc aggctttagt caagtacagt agttcagaag cgcaggcggc
    42121 ctatgattca gctagtcgag cagtagctaa ggcagatcgt catatcaatg aactcaatca
    42181 agcacgaaat gaagccgctt cagctccggc tccacagtta ccagcgccag taggaggaga
    42241 agatgcaacg gtgcaaagcc caactccagt ggctggaaat tctgttgctt ctattgacgc
    42301 tcaattgggt gatgcccgtg atgcgcgtgc agatgctgcg gcgcaattaa gcaaggctca
    42361 aagtcaattg gatgcaacaa ctgttctcag taccctagag ggaactgtag tcgaagtcaa
    42421 tagcaatgtt tctaaatctc caacaggggc gagccaagtt atggttcata ttgtcagcaa
    42481 tgaaaattta caagtcaagg gagaattgtc tgagtacaat ctagccaacc tttctgtagg
    42541 tcaagaagta agctttactt ctaaagtgta tcctgataaa aaatggactg ggaaattaag
    42601 ctatatttct gactatccta aaaacaatgg tgaagcagct agtccagcag ccgggaataa
    42661 tacaggttct aaataccctt atactattga tgtgacaggc gaggttggtg atttgaaaca
    42721 aggtttttct gtcaacattg aggttaaaag caaaactaag gctattcttg ttcctgttag
    42781 cagtctagta atggatgata gtaaaaatta tgtctggatt gtggatgaac aacaaaaggc
    42841 taaaaaagtt gaggtttcat tgggaaatgc tgacgcagaa aatcaagaaa tcacttctgg
    42901 tttaacgaac ggtgctaagg tcatcagtaa tccaacatct tccttggaag aaggaaaaga
    42961 ggtgaaggct gatgaagcaa ctaattagtc taaaaaatat cttcagaagt taccgtaatg
    43021 gtgaccaaga actgcaggtt ctcaaaaata tcaatctaga agtgaatgag ggtgaatttg
    43081 tagccatcat gggaccatct gggtctggta agtccactct gatgaatacg attggcatgt
    43141 tggatacacc aaccagtgga gaatattatc ttgaaggtca agaagtggct gggcttggtg
    43201 aaaaacaact agctaaggtc cgtaaccaac aaatcggttt tgtctttcag cagttctttc
    43261 ttctatcgaa gctcaatgct ctgcaaaatg tagaattgcc cttgatttac gcaggagttt
    43321 cgtcttcaaa acgtcgcaag ttggctgagg aatatttaga caaggttgaa ttgacagaac
    43381 gtagtcacca tttaccttca gaattatctg gtggtcaaaa gcaacgtgta gccattgcgc
    43441 gtgccttggt aaacaatcct tctattatcc tagcggatga accgacagga gccttggata
    43501 ccaaaacagg taaccaaatt atgcaattat tggttgattt gaataaagaa ggaaaaacca
    43561 ttatcatggt aacgcatgag cctgagattg ctgcctatgc caaacgtcag attgtcattc
    43621 gggatggggt catttcgtct gacagtgctc agttaggaaa ggaggaaaac taagatgcag
    43681 aatctgaaat ttgccttttc atctatcatg gctcacaaga tgcgttcttt gcttactatg
    43741 attgggatta ttatcggtgt ttcatcagtt gttgtgatta tggctttggg tgattcccta
    43801 tctcgtcaag tcaataaaga tatgactaaa tctcagaaaa atattagcgt ctttttctct
    43861 cctaaaaaaa gtaaagacgg gtcttttact cagaaacaat cagcttttac ggtttctgga
    43921 aaggaagagg aagttcctgt tgaaccgcca aaaccgcaag aatcctgggt ccaagaggca
    43981 gctaaactga agggagtgga tagttactat gtaaccaatt caacgaatgc catcttgacc
    44041 tatcaagata aaaaggttga gaatgctaat ttgacaggtg gaaacagaac ttacatggac
    44101 gctgttaaga atgaaattat tgcaggtcgt agtctgagag agcaagattt caaagagttt
    44161 gcaagtgtca ttttgctaga tgaggaattg tccattagtt tatttgaatc tcctcaagag
    44221 gctattaaca aggttgtaga agtcaatgga tttagttacc gggtcattgg ggtttatact
    44281 agtccggagg ctaaaagatc aaaaatatat gggtttggtg gcttgcctat tactaccaat
    44341 atctcccttg ctgcgaattt taatgtagat gaaatagcta atattgtctt tcgagtgaat
    44401 gataccagtt taaccccaac tctgggtcca gaactggcac gaaaaatgac agagcttgca
    44461 ggcttacaac agggagaata ccaggtggca gatgagtccc ttgtatttgc agaaattcaa
    44521 caatcgttta gttttatgac gacgattatt agttccatcg cagggatttc tctctttgtt
    44581 ggaggaactg gtgtcatgaa catcatgctg gtttcggtga cagagcgcac tcgtgagatt
    44641 ggtcttcgta aggctttggg tgcaacacgt gccaatattt taattcagtt tttgattgaa
    44701 tccatgattt tgaccttgtt aggtggctta attggcttga caattgcaag tggtttaact
    44761 gccttagcag gtttgttact gcaaggttta atagaaggta tagaagttgg agtatcaatc
    44821 ccagttgccc tatttagtct tgcagtttcg gctagtgttg gtatgatttt tggagtcttg
    44881 ccagccaaca aggcatcgaa acttgatcca attgaagccc ttcgttatga atgagatcaa
    44941 caagatggac actcgtctat cttgtttttg tatggatttc cctatcgaaa ttcattaaaa
    45001 atgtgatata atgaagtgtt agaaaaacag gtttcatttg cctggaaagg aaaaattatg
    45061 tctgaaaaaa atttttatat tacaacgccg atttactatc catctgggaa acttcatatc
    45121 ggttctgcct acacaactat cgcatgtgat gtcctagcac gttacaaacg cctgatgggc
    45181 tacgatgtat tttatctgac aggtcttgat gaacatggtc agaaaatcca gcagaaagcg
    45241 gaagaagctg gtattacacc tcaagcctat gttgatggaa tggcggttgg agttaaagaa
    45301 ctctggcaat tactagatat ctcatacgat aaatttatcc gtacaactga tgactaccat
    45361 gaaaaagttg tcgcacaggt ctttgaacgc ttacttgctc aagatgatat ctacttgggt
    45421 gaatactctg gttggtattc agtatcagac gaagaattct ttacagaaag ccagctggca
    45481 gaagttttcc gtgatgaagc tggaaatgtg actggtggta ttgctccatc aggtcatgag
    45541 gttgaatggg tttctgaaga atcatacttc cttcgcctta gcaaatacca agaccgtttg
    45601 gtcgaatttt tcaaagctca tcctgaattt atcacgccag atggtcgcct taatgaaatg
    45661 ctacgcaact tcatcgagcc aggtttggaa gatttggcgg tatctcgtac aacctttaca
    45721 tggggagtgc ctgtcccatc aaatccaaaa cacgttgtct acgtttggat tgatgccctt
    45781 cttaactatg cgacagctct tggctacgct caagacgaac atggtaactt tgacaagttc
    45841 tggaatggaa cagtcttcca tatggtagga aaagatatcc ttcgcttcca ctctatctac
    45901 tggccaatcc ttcttatgat gttggatgtt aaattacctg atcgtttgat tgcccatggt
    45961 tggtttgtca tgaaagacgg aaaaatgtct aagtcaaaag ggaatgtcgt ttaccctgaa
    46021 atgttggtag agcgttatgg actagatcca cttcgttact acctcatgcg taaccttcca
    46081 gttggttcag acggaacctt tactcctgaa gactatgtcg gccgtatcaa ctatgaattg
    46141 gctaatgacc ttgggaacct ccttaaccgt acggtttcca tgattaataa gtactttgat
    46201 ggacaaatcc ctgcctatgt agaaggtgtg actgaatttg atcatgttct tgctgaggtt
    46261 gcagaacaat caatcgcaga cttccataca cacatggaag cagttgacta tccacgtgcg
    46321 cttgaagcag tctggactct gatctctcgt accaataaat acatcgatga gactgcacca
    46381 tgggtcttgg acaaggatga agctcttcgt gaccaattgg caagtgtcat gagccacttg
    46441 gcagccagca ttcgtgtagt tgctcacttg attgaaccat ttatgatgga aactagtcgt
    46501 gcagttttga ctcaacttgg tttggaagaa gtttctagtc ttgaaaactt gagtttggct
    46561 gacttcccag cagatgtgac tgtagttgcc aaaggaacac ctatctttcc acgtctaaat
    46621 atggaagaag aaatcgccta tatcaaggaa caaatggaag gcaataaacc agcagtcgaa
    46681 aaagaatgga atccggacga agttgagctc aaactaaaca aggatgaaat caagtttgaa
    46741 gactttgaca aggttgaaat ccgtgtcgca gaagtcaaag aagtgtctaa agtagaaggt
    46801 tcagataagt tgcttcaatt ccgcttggat gctggtgatg gagaagatcg tcagattctt
    46861 tcaggaattg caaaatacta tccaaatgaa caagaattgg tcggcaagaa agttcaaatc
    46921 gttgctaacc tcaaaccacg taaaatgatg aaaaaatatg tcagccaggg tatgattctc
    46981 tcagctgaac atgatggcaa attaaccctt ctcacagttg atccagctgt accaaatgga
    47041 agtgtgattg ggtaaaagta gacaggacta gttcaggcta gttctgtttt tttatttata
    47101 ctcaatgaaa atcaaagagc aaactaggaa gctagccgcg ggttgatcaa agcactgctt
    47161 tgaggttgta gatagaactg acgaagtcag ctcaaaacat ggttttgagg ttgtagatga
    47221 aactgacgaa gtcagctcaa aacactgttt tgaggttgta gatgaaactg acgaagtcag
    47281 taaccataca tacggtaggg cgacgatgac gtggtttgaa gagattttcg aagagtatta
    47341 actattatgc tttacaaact agtgtagaag tgatatacta taggcgaagt tactagtagg
    47401 tattacaaaa tagattagaa gggagaagtg atgaaagaaa ctcagctatt aaaaggtgtt
    47461 cttgaaggtt gtgtcttgga tatgattggt caaaaagagc ggtatggtta tgagttggtt
    47521 cagactttgc gagaggctgg atttgatact atcgttccag gaactattta tcctttgttg
    47581 caaaagttag aaaaaaatca atggataaga ggcgacatgc gcccgtcgcc agatggtcca
    47641 gatcggaagt atttttcatt aatgaaagaa ggagaagagc gtgtctcagt cttttggcaa
    47701 caatgggacg atttgagtca aaaagtagaa gggattaaga atgggggtta aaccatgaag
    47761 aaaatgaagt attacgaaga aacaagcgct ttgctacatg agttttctga ggagaatcaa
    47821 aagtattttg aggagttgtg ggaaagtttt aatcttgctg gatttctcta tgatgaagac
    47881 tatctcagag agcagatcta tttgatgatg ctagatttct cagaagcaga acgagatggc
    47941 atgagtgcag aggattatct aggtaagaat cctaaaaaaa taatgaaaga gattctcaag
    48001 ggagcacctc gcagttctat caaagagtcc cttttgacgc caattcttgt cctggcggta
    48061 ttacgttatt atcaactact aagtgatttt tctaaaggtc ctctcttaac agtcaatttg
    48121 ctcacatttt tagggcaact tcttattttt ctgattggat ttggacttgt ggccacaatt
    48181 ttacgaagaa gtttagtcca agattctcct aaaatgaaaa ttggcactta cattgttgtt
    48241 gggactatag ttcttctagt tgttttagga tatgtaggaa tggcaagctt catacaagaa
    48301 ggagcctttt atattccggc tccctgggat agtttgtctg tctttacgat ttcgctagtt
    48361 atcggtattt ggaattggaa agaagcggtc tttcgtccat ttgtcagtat gattattgcc
    48421 catcttgtgg tgggttctct gctccgttat tatgagtgga tgggaatttc aaatgttttc
    48481 cttacaaaag ttattccttt agctgtcctc tttattggaa tctttgtctt gttccgtggg
    48541 tttaagaaga taaaatggag tgaagtatag tcaaaaagcc gttgcaaagc ggttttttgt
    48601 tataatgaag ggaagaatgt tagaaattaa aggagaaaaa tttgatgaga tacataactc
    48661 ttggtcaaga tgacaaagaa ttatcagaaa ttgttctcgg aatgatgaga ataaaagata
    48721 agtctgtaaa agaagttgaa gagcttgtag aaacagcact ttctgttgga atcaatgcct
    48781 tcgacttggc tgatatttat ggtcgtggtc gttgtgaaga actgttaggt cttgtcctaa
    48841 aaaatcgtcc agatttaaga gaaaagatgt ggattcagtc caaatgtggc attcgcattg
    48901 aagaatttac ctattttgat ttttctaagg actatattat aaaatcagta gacggtattt
    48961 tgcaaagatt gaagattgat catctagata gcttgctcct tcatcgacca gatgctttga
    49021 tggaatctga ccaagtagca gaagcctttg atctccttta taaacaaggt aaagttcgag
    49081 attttggagt ttctaatcaa aatcctatga tgatggagtt gcttaaaaaa gatgtcaagc
    49141 agccgttagc tgttaatcag ctacaattga gtgcggcttt tactccagga tttgaatcag
    49201 cttttcatgt taatatggaa gatagtcaag cagctatgcg agatggcagc atttttgaat
    49261 attgccaatt acacgatgtg gtcattcaag catggtctgt cttgcaattc gggtatttta
    49321 aagggaactt tgttggaaat gagaaatttc aagctttaaa tcaagtactt gatcgtttag
    49381 ctattaaata tggagtaacc tcttcaacta ttgccatttc ttggatattg cgttatccag
    49441 caaaaatgca ggcagtcgta ggtacaacaa atcctaagca cttgagagaa gttagccgag
    49501 cggcaaactt tagcttaaca agaaaagaat ggtatgaaat ttatcttgct gcagggaata
    49561 atttgccgta agtagtagca ggtttgaata actcacattc aaaaagccgt ataaagcggt
    49621 tttttgctat aattaaatga agaatgtaga attaaaggag aaaaacatga catttgaaga
    49681 gatcctgcct gggttaaagg ctaagagaaa atatgtacga actggttggg gaggtgcgga
    49741 aaactatgtc caactctttg ataccatcga gcaaaatggg cttgcacttg aaatgacacc
    49801 ttatttccta atcaacgttt ctggcgaagg agaaggtttt tccatgtgga gcccgacagt
    49861 ttgtgatgtt ttggcaacgg attgggtaga agtgcatgac taaacgtgta ctcattacag
    49921 gagtgagttc agggatcgga ttggctcaag ctcgcctctt tttagagaag ggctatcaag
    49981 tttatggagt tgaccaaggt gaaaagtcac tcttagaggg tgattttcac tttttacaga
    50041 gagatttgac cttggacttg gaacctattt ttgactggtg ccctcaggtg gatgttttgt
    50101 gtaatactgc tggagttttg gatgattaca aaccactgtt ggaacaaacg gcgcaggaca
    50161 ttcaagcgat ttttgaaatc aactacatta ttccagtaga gttgactcgg tattatttga
    50221 cacaaatgct ggaaaataaa aagggaatca tcatcaatat gtgttccatt gcttctagcc
    50281 tagcaggtgg aggtggtcac gcctatactt catccaagca tgccttggct ggcttcacca
    50341 agcagttggc tctagactat gctgaagctg ggattcaggt ctttggtatc gctccaggag
    50401 cagtcaagac agctatgacc gctgcggatt ttgaaccagg tggtttggct gactgggtgg
    50461 ctagtgaaac cccaatcaag cgctggattg agccagagga aatagcagag cttagtcttt
    50521 ttttagcaag tggaaaagcg agcgccatgc aaggacaaat cttgacaata gatggtggct
    50581 ggtctttgaa gtaggaggag ttggatggaa atcaaaaaac actttggagt ctacgctgtt
    50641 tgcttagaaa atgggaagtt actctgcatt gaaaaaacga gaggccctta tcaacatcgg
    50701 tatgatctac ctggaggcag tcagcaactt ggtgaaggac tgacggaaac gctgactaga
    50761 gaagttatgg aagagacggg atttactgtt agaagctact ctaatcctcg aatctacgat
    50821 gttttcgtta gggaagagtt aaaaaatttt atggttcacc atgtcatggc cttgtatgat
    50881 gttgaaatga atgagagtgc acctcaagtt acgacttcgg aagctgtgtc tgatggtgcg
    50941 aatgattcac ttggatatat ttggatggat attcaagaga ttacagaaga aaatgcatcg
    51001 ccattagtct tgaaggttaa gtctgaatta ttaggatttc cagaactgga caagacttct
    51061 tacatgaatt ggaaggtgaa tgatgaaaaa tcaacttgcc cttagtggcg aaaaaattct
    51121 tgaaaaaatt tatcctcaac tatttcacca tgtcggcatg attcgtgggg aatacttatt
    51181 gagagagctc aatcaaaata tactattaca aagttgtcag gaatttgtaa aagattatct
    51241 agagactatc tgctccctgt actcagatga agaagtttgg tatcgtttta ctgaattaac
    51301 gaatacagaa gccaattgtt tagtggggac taaagagttt tttgacgaag ggcatccctt
    51361 gtttggctat agaggaacca ggcgtttact ggcgtgtttg gatgaatttc aggctgaagc
    51421 acatgtcgtt acagaagttt atcaaaccaa ccccaatcta tctgttatct ttccttttgt
    51481 caatgatgcc gaccaattaa aacaagctat tacagtattg cgtcagtatg gctttactgg
    51541 gaaagtcgga acgatgattg aattaccgtc agcgtatttt gacttatcta gtatactgga
    51601 aacgggcatt tcaaagattg tagttggaat gaatgatttg acttcctttg tttttgcgac
    51661 tatgagaaac agtcaatggc atgatctgga aagtccaata atgttagata tgctaagaga
    51721 tatgcaggat aaagcaagaa agaacaagat taattttgct gtagcaggct atctgaatac
    51781 ttctttcata caaaaaatga atcagttggg tatcaagtgc attatccact acagttctat
    51841 tccagagatt tttgatttag agattgacca tccagatcat cttaaacaca taaaagaaga
    51901 aagtaaaaaa ttacaaagga gcacccatga caccgcaaga aatgtggaat aaatacaaac
    51961 aaatcaatcc ttctatcgga gatgaaatcg atgcctgggc ttttggagtt gaaccagacc
    52021 ttttagcgga tttggtgttt aaaggagaaa agacagcaac agcatcagcc tatgacctct
    52081 atgtactaga agacgaacca cttccccaag tagggacctt cgatgtcatt ttagatagtc
    52141 aaaatcaggc tgtctgcatt gtcgaaatta caaaggtttc tgttaaaccc ttcaatcgag
    52201 tgtcagctga ccatgcctat aaggaaggtg agggaaacaa gacacttgtc tattggcgcc
    52261 aggttcatga ggattttttc agagactgct tgggtgaagc aggattgact tttacacctg
    52321 aaagcaaggt tgttttagaa gaatttcgca aggtctaccc actgtagact attagaagga
    52381 agaaagtttt ggaaatcgct gtccaatcct tttttctcaa gcaaaatatg atataataag
    52441 tttgtttgaa gaagagcagc agctcttaaa cttagaatag gagaaaacta tgcaagcagt
    52501 tgaacatttt attaagcaat ttgttcctga acattatgat ttatttttag atttgagtcg
    52561 tgagaccaag actttttctg ggaaagtgac catcactggt caagcacaga gtgaccgcat
    52621 ctccctccac caaaaagact tggaaatcac ctctgtagaa gttgcaggtc aagctcgtcc
    52681 atttacagtt gaccatgaca atgaagccct tcatatcgaa ttggctgagg ctggtcaagt
    52741 tgaattggtt cttgcctttt ctggtaaaat tacagacaac atgacaggga tttacccttc
    52801 ttattataca gttgatggag tcaagaagga ggtcttgtct actcagttcg agagccattt
    52861 tgcgcgcgaa gctttcccat gtgtggatga gccagaagcc aaagcaacct ttgatctcgc
    52921 tcttcgcttt gaccaagcag caggtgagtt ggccttgtca aacatgcctg agattgatgt
    52981 ggataaccgc aaggaaacag gtatttggaa gtttgagaca acacctcgta tgtcatctta
    53041 cttgttggcc tttgtagctg gtgatttgca aggtgtgacg gctaaaacta aaaacggtac
    53101 cctggtaggt gtctactcaa ccaaagcaca tccactttca aatcttgatt tctcactgga
    53161 tatcgctgtt cgctctatcg agttttacga agattactac ggtgttaaat acccaatccc
    53221 tcaatctctc cacatcgccc ttcctgactt ctcagctggt gctatggaaa actggggtct
    53281 tgtgacctac cgtgaagttt acttggttgt cgatgagaac tctacatttg ctagccgtca
    53341 acaagttgcc cttgttgtgg cccatgaatt ggctcaccaa tggtttggga acctcgtgac
    53401 tatgaaatgg tgggatgacc tttggctcaa tgaaagtttc gctaatatga tggaatacgt
    53461 ctgtgtggat accatcgaac caagctggaa tatctttgaa gatttccaaa caggtggagt
    53521 acctcttgct cttgaacgtg acgctactga tggcgttcag tctgtccacg tcgaagttaa
    53581 acatccagat gaaattaata cactctttga cggcgctatc gtctatgcca aaggaagccg
    53641 tctcatgcac atgcttcgcc gttggctagg agatgctgat tttgctaaag gtttgcacgc
    53701 ctactttgaa aaacaccaat acagcaacac cattggtagt gacctttggg atgcccttgg
    53761 tcaagcgtca ggacgtgatg tcgcagcctt catggattct tggttggaac agcctggtta
    53821 cccagttctt actgtcaaag ttgaaaatga tgtcttgaag atttcacaaa aacaattctt
    53881 catcggtgag aacgaagaca agaaccgtct ctgggtggtg cccctcaata gcaactggaa
    53941 aggcttgcct gatacactcg aaactgaaag tatcgaaatc cctggctacg cagctcttct
    54001 tgctgagaat gaaggagctc ttcgtctcaa cactgaaaat actgcccact atattaccga
    54061 ctatcaagga gacttgttag aagctgttct tgctgagcta gagacacttg ataacacaag
    54121 caaactgcaa attgttcaag aacgtcgttt gttggctgag gcagggcaca tttcttatgc
    54181 agacttgctt ccagttcttg ataaacttgc taaggaagag tcttatctgg tggtttcagc
    54241 tgtttctcaa gtgatttcag cccttgagcg ctttattgat gaaggaacgg atgctgaaac
    54301 agccttcaaa ggactggttg ctaaattggc tcgtcataac tatgaccgtc ttggttttga
    54361 agctaaagac ggagaatcag atgaggatga attggttcgt cagctggctg tttcgatgat
    54421 gattcgctca aatgatgcag aagctagtca agtcgctagc caaatcttcg caacacacaa
    54481 ggagaatctt gcaggactcc cagcagctat tcgctcacaa gttctgatta atgagatgaa
    54541 acatcatgag actaaagact tgttagcact ttatcttgat acttatactc acgcaacaga
    54601 tgctgtcttt aaacgccagt tggcagcagc tcttgcatac agtacagatg cggataatat
    54661 ccaaaacttg attacttctt ggaaggacaa atttgtggtc aaaccacagg atttgtctgc
    54721 ttggtattac caattcctag ctcatcaagc aactcagaaa acagcttggt cttgggcgcg
    54781 tgaaaactgg gcttggatta aggcagccct tggaggagat atgagctttg atagctttgt
    54841 tatccttcct gctcatgtat ttaagactca gcaacgcttg gcggaataca aggagttctt
    54901 tgagccgcaa ctttctgacc ttgctcttag ccgtaacatc ggtatgggaa tcaaggaaat
    54961 tgcagcacgt gttgacttga ttagccgtga aaaagctgca gtcgaagcag ttgttcttca
    55021 atacggaaat gcataaataa gcctaaaata aaaagaaaac tcagctatct catgtaaaat
    55081 gcagagagtt gagttttttt taaggcaaat ttggtataat agttttaata aggaggagtt
    55141 tctcatgata aaaatcttat tggttgagga tgacctaggt ctgtcaaatt cagtatttga
    55201 ctttttagac gattttgcgg atgttatgca ggtatttgat ggagaagaag gtctctacga
    55261 agctgagagt ggtgtctatg atttgatttt gctggatttg atgttgccag aaaaaaatgg
    55321 tttccaagtc ttaaaagaat tgcgtgaaaa gggaattacg acaccagttc tgattatgac
    55381 tgccaaggaa agtttggatg acaagggaca tggatttgaa ctgggagcgg atgattatct
    55441 gaccaaacct ttctacctag aagaacttaa aatgcggatt caggcccttc tcaaacgttc
    55501 agggaagttt aatgaaaaca ccttgactta tgggaatatc gtggttaatt tatcaaccaa
    55561 taccgttaaa gttgaagata ctcctgtcga attgctgggg aaagagttcg atttactagt
    55621 ttatttcctt caaaatcaaa atgtgatttt gcctaagacg cagatttttg accgtctatg
    55681 gggatttgat agtgatacaa cgatttcggt tgtcgaagtc tatgtttcaa aagtccgtaa
    55741 gaaattaaag ggaaccactt ttgcagagaa tttgcaaacc ttgcgtagtg ttgggtatct
    55801 tttaaaagat gttcagtaaa cttaaaaaaa catggtatgc ggatgacttt agttatttta
    55861 tccgcaactt cggtgtcttc accctgattt tttctacaat gactctgatt attttacaag
    55921 tcatgcattc gagtctttat acttcggtgg acgataagct tcatggactg agtgaaaatc
    55981 ctcaagcagt tattcagctg gctataaata gggcaacaga agagattaaa gatttagaaa
    56041 atgctagggc ggacgctagt aaagtagaaa taaaacctaa tgtcagttcc aatacggaag
    56101 ttattctctt tgataaggac tttactcaac ttctttctgg aaatcgattt ttgggcttgg
    56161 ataagattaa gttagaaaag aaagaactag gacatatcta ccagattcag gtttttaata
    56221 gctatgggca ggaagaaatc tatcgtgtga ttttgatgga gaccaatatt agttcggttt
    56281 caaccaatat caagtatgct gctgtcttga ttaataccag tcagttggag caggctagtc
    56341 aaaagcatga gcaattgatt gtggtcgtga tggctagttt ctggattttg tctttacttg
    56401 ccagtctcta tctagctagg gtcagtgtta ggcccctgct tgagagtatg cagaagcaac
    56461 agtcttttgt ggaaaatgcc agtcatgagt tacgaactcc actcgcagtt ttgcaaaatc
    56521 gcttagagac cctttttcgt aagccagaag ctaccattat ggatgtgagc gaaagcattg
    56581 catcgagttt ggaagaagtc cgaaatatgc gttttttaac gacaagcttg ctgaacttag
    56641 ctcggagaga tgatgggatt aagccggagc ttgcagaagt tccaactagc ttttttaata
    56701 caactttcac aaactatgag atgattgctt cggaaaataa tcgtgtcttc cgttttgaaa
    56761 atcgtatcca tcgaacaatt gtcacagatc agcttcttct gaaacaactg atgaccattc
    56821 ttttcgataa tgccgtcaag tatactgagg aggatggtga aattgatttt cttatctcgg
    56881 cgaccgatcg caatctttat ttacttgttt ctgataatgg aatcggtatt tcgacagaag
    56941 ataaaaagaa aatttttgac cgtttttatc gagtagacaa ggctagaacc cggcaaaaag
    57001 gtggttttgg tttaggatta tccctagcca agcaaattgt agatgctcta aaaggaactg
    57061 ttactgtcaa agataataaa cccaagggaa caatctttga agtgaagatt gccattcaga
    57121 caccatctaa aaagaaaaaa taaaaatatc gctccaattg gggcgatatt ttggatttat
    57181 tttctacgtt ttcgtttgat aatagaccgt tgaactttta aaacaagtaa gctgaatccg
    57241 attgctgcgg caaaggcaag agcagttgat aattttaatg ctaaaaagat aaaactaaag
    57301 atagcaatac agatacaaaa aacagcgata ttaataaaaa ataggatttc cttgagattg
    57361 gcatcagatt gcgcttcagg tgtataagct tggtaatgag gaagctgctg gtttaattct
    57421 tcttgatagt ctacctcata ggattgtaat tttcttacgg gcatgattct ctccttaaca
    57481 gtacatacct attttatcat tttttcggca gagaattatt acagaaaggt tacaaaaaga
    57541 ataaagtccc ttttcatttt caaagcatgg ctgattttgg agaaatgtgg tataattttt
    57601 cttatggaaa agattgtcat tacagcaact gctgaaagta ttgaacaagt tgaacaacta
    57661 ctcgaagctg gcgtagaccg tatctatgtc ggtgagaaag attttggcct tcgtctgcca
    57721 acgaccttta gttatgacca attacgtgaa atcgctaagt tggttcatga tgctggtaag
    57781 gaattgatcg ttgcggtcaa tgctctcatg caccaagata tgatggaccg tatcaagcct
    57841 ttcttagact tattggaaga aatcaagaca gactatatta cgattgggga tgcaggcgtc
    57901 ttttacgtag tcaaccgcga tggttattca tttaagacca tctacgatgc ttcaaccatg
    57961 gtaactagca gtcgtcagat taacttctgg ggacaaaagg ctggtgcatc tgaggctgtt
    58021 ttggcgcgtg aaattccatc agctgaactt ttcaaaatgc cagagatttt ggaaattcct
    58081 gctgaagttt tggtttacgg tgctagtgtc atccatcatt ctaaacgtcc gctcttgcaa
    58141 aactactata actttacaca tatcgatgat gaaaagacgc ataaacgtga cctcttcttg
    58201 gctgagccaa gtgatccaga gagccactat tccatttttg aagataatca tgggacccat
    58261 atctttgcca acaatgacct tgatttgatg atcaaattaa cagaattggt ggagcatggc
    58321 tttactcgct ggaaactaga agggctctac actcctggtc agaactttgt tgagattgca
    58381 aaactcttta tccaagcgcg tagcttgatt caagagggca actttagcca tgaccaagcc
    58441 ttcttgctgg atgaagaagt tcgtaaactt caccctaaaa accgtttcct tgatacagga
    58501 ttttatgact acgatcctga catggttaga taaaatacat gattcgttga gagaaggaag
    58561 atgcaaacat ttcttctctc aatttttcgt atttcttcac tattttacaa aaatcagcag
    58621 gctagaatgc tctattcgat gggattttta agaaaagtag tgttcttgag tttgaaaatt
    58681 atcctatgtt tgcaggtgcc aaatggccct ttttttggta taatttttta taatgaaaac
    58741 gattggtaat cgctatgttg tggtggattt agaggcaact agcacaggta gtaaggctaa
    58801 aattatccaa gtgggaattg tcgtgattga ggacggagaa atcgtcgatc actatacgac
    58861 ggatgtcaat ccacatgaac ccttggatgc tcatatcaaa gaactgacag gattgacaga
    58921 ccaacgtctg gcgcaagcac ctgatttttc gcaagttgcc agaaaaatat ttgacttggt
    58981 ggaggatggg atttttgtag cccataatgt tcagtttgat gctaatctct tggcggaaaa
    59041 tttatttttt gaaggctatg agctaagaaa ccctcgtgtt gatacggtcg aattggccca
    59101 ggtctttttc cctgaactgg aaaaatatag cttgccgatt ttgtgtcgag aattaggaat
    59161 tcctcttaaa cacgcacaca cagccctttc agatgcccaa gctacagcag aattacttct
    59221 ttttttacgg gaaaagatga cccagcttcc taaaggtctc ttggaacgct tgctggaaat
    59281 ggctgacgct ctcctatatg agtcctatct ggttattgag gaaacttatc gcaaccaatc
    59341 tatcctgagt tctccagact tggtccaagt tcaaggtcta tattttaaga aaacggaagc
    59401 ttctctggag ccacgaaaac tatctcaaga cttttctaaa aatatttctc tgttgaacct
    59461 tgaagtgagg gaggaacaag aaagttttgc taaagaggtt ggcttgctat tgaaagatga
    59521 acctgtctct ctgattcaag cgccgacagg gattgggaaa acctatggct atctcttacc
    59581 cgctttatct caatccaaag agcgacaaat tgttcttagt gttccgacaa agattcttca
    59641 aaatcaaatc atggaagaag aaggtaaacg cctcaaggaa gtgttccata cagatattca
    59701 tagcttaaag ggaccacaaa attatctgaa gttggatgcc ttttatcatt ccttgcagga
    59761 aaatgatgaa aatcgcttat ttagacgctt taaaatgcaa gtcttggtct ggcttactga
    59821 gacagagaca ggagatttgg atgaaatcgg gcaactctac cgttaccaac attttctagc
    59881 agaccttcgt catgatggga atttatcatc ccagagctta tttgtgacgg aagatttttg
    59941 gaaacgtagt caagaaaggg cagagacttg caagctttta gtgactaatc atgcctatct
    60001 cgtaaccaga cttgaagata atcctgaatt tgtcagtgac cgtttactga ttattgatga
    60061 agtccaaaag attttgttag ctctagaaaa tctgcttcaa gagacctacg atatacaatc
    60121 tattatcgat ttaattgata aggctttagt aggagaagaa aacagggttc aacaacggat
    60181 actagaaagt attcgctttg agtgtctcta cttgatagaa caatttcagt ctggcaaatc
    60241 taggaaaaat atcttagatt ctctggacaa tctccatcag tatttttcag aattggaagt
    60301 agaagacttt gatgagctgg ttcgctattt tacagctgaa ggtgattact ggcttgaagt
    60361 aactgaaacg agtcaaaaga aaattcagat ttcttctaca aaatcaggcc gtactcttct
    60421 gtcctcttta cttcctgaga gttgccaagt cttgggagta tcggctactc ttgagattag
    60481 tcagagggtt tctttggcag accttttagg ctatcctgaa gctaaatttg tcaagattga
    60541 atctcgggga aaacaggaac aagaagtggt catggtcaaa gatttccctc tggtaacaga
    60601 aacctcctta gaagtctatg ccagagaggt agctgcttta ctagtggaaa ttcaagcttt
    60661 ccagcaaccg attttggttc tctttaccgc taaagacatg cttctagcag tatcggattt
    60721 acttacagtt agccacttgg cccagtataa aaatggggat gttcatcagc taaagaaacg
    60781 ctttgaaaaa ggtgaacaac aaatcttgct tggtgcagca agtttctggg agggagttga
    60841 tttttcaagc catccttctg tgattcaagt tgtaccgagg cttcctttcc aaaatcctca
    60901 agaacccttg acgaaaaaga ttaatcaaga actgaatcaa gaagggaaaa atgcctttta
    60961 tgattatcaa ttgccaatgg ccattattcg tttaaaacag gctttgggaa gaagtatgag
    61021 acgtgaatac caacgttcct taactcttat tttggatagg agaatcgtcg gaaaacgata
    61081 cggcaaacaa atagtagcat ctctagcaga agaagcaact gttaaaacca tctctcgatc
    61141 cgaagttgac gaggctattg atagattttt taatgaactt tgataaatag tattgtatga
    61201 aagtataagg ttagtatata tgaaacgttc tctcgactct agagtcgatt atagtttgct
    61261 cttgccagta ttttttctac tggtcatcgg tgtggtggct atctatatag ccgttagtca
    61321 tgattatccc aataatattc tgcccatttt agggcagcag gtcgcctgga ttgccttggg
    61381 gcttgtgatt ggttttgtgg tcatgctctt taatacagaa tttctttgga aggtgacccc
    61441 ctttctatat attttaggct tgggacttat gatcttgccg attgtatttt ataatccaag
    61501 cttagttgca tcaacgggtg ccaaaaactg ggtatcaata aatggaatta ccctattcca
    61561 accgtcagaa tttatgaaga tatcctatat cctcatgttg gctcgtgtca ttgtccaatt
    61621 tacaaagaaa cataaggaat ggagacgcac ggttccgctg gactttttgt taattttctg
    61681 gatgattctc tttaccattc cagtcctagt tcttttagca cttcaaagtg acttggggac
    61741 ggctttggtt tttgtagcca ttttctcagg aatcgtttta ttatcagggg tttcttggaa
    61801 aattattatc ccagtatttg tgactgctgt aacaggagtt gctggtttct tagctatctt
    61861 tattagcaag gacggacgag cttttcttca ccagattgga atgccgacct accaaatcaa
    61921 tcggattttg gcttggctca atccctttga gtttgcccaa acaacgactt accagcaggc
    61981 tcaagggcag attgccattg ggagtggtgg cttatttggt cagggattta atgcttcgaa
    62041 tctgcttatc ccagttcgag agtcagatat gatttttacg gttattgcag aagattttgg
    62101 ctttattggc tctgtcctgg ttattgccct ctatctcatg ttgatttacc gtatgttgaa
    62161 gattactctt aaatcaaata accagttcta cacttatatt tccacaggtt tgattatgat
    62221 gttgctcttc cacatctttg agaatatcgg tgctgtgact ggactacttc ctttgacggg
    62281 gattcccttg cctttcattt cgcaaggggg atcagctatt atcagtaatc tgattggtgt
    62341 tggtttgctt ttatcgatga gttaccagac taatctagct gaagaaaaga gcggaaaagt
    62401 cccattcaaa cggaaaaagg ttgtattaaa acaaattaaa taaggagaaa atcatggtaa
    62461 aagtagcagt tatgttagct cagggctttg aagaaattga agccttgaca gttgtagatg
    62521 tcttgcgtcg agccaatatc acatgtgata tggttggttt tgaagagcaa gtaacgggtt
    62581 cgcatgcaat ccaagtaaga gcagatcatg tctttgatgg agatttatca gactatgata
    62641 tgattgttct tcctggaggt atgcctggtt ctgcacattt acgtgataat cagaccttga
    62701 ttcaagaatt gcaaagcttc gagcaagaag ggaagaaact agcagccatt tgtgcggcac
    62761 caattgccct caatcaagca gagatattga aaaataagcg atacacttgt tatgacggcg
    62821 ttcaagagca aatccttgat ggtcactacg tcaaggaaac agtagtggta gatggtcagt
    62881 tgacaaccag tcggggtcct tcaacagccc ttgcctttgc ctacgagttg gtggagcaac
    62941 taggagggga cgcagagagt ttacgaacag gaatgctcta tcgagatgtc tttggtaaaa
    63001 atcagtaaaa cgggagttat tctctcgttt tttatgtgga aaactcaggg aaatcatcgc
    63061 ttttttcata aaaaaatgct ataatgaagg gtatgaaata tcacgattac atctgggatt
    63121 taggtggaac tttactggat aattatgaaa cttcaacagc tgcatttgtt gaaacattgg
    63181 cactgtatgg tatcacacaa gaccatgaca gtgtctatca agctttaaag gtttctactc
    63241 cttttgcgat tgagacattc gctcccaatt tagagaattt tttagaaaag tacaaggaaa
    63301 atgaagccag agagcttgaa cacccgattt tatttgaagg agtttctgac ctattggaag
    63361 acatttcaaa tcaaggtggc cgtcattttt tggtctctca tcgaaatgat caggttttgg
    63421 aaattttaga aaaaacctct atagcagctt attttacaga agtggtgact tctagctcag
    63481 gctttaagag aaagccaaat cccgaatcca tgctttattt aagagaaaag tatcagatta
    63541 gctctggtct tgtcattggt gatcggccga ttgatatcga agcaggtcaa gctgcaggac
    63601 ttgataccca cttgtttacc agtatcgtga atttaagaca agtattagac atataagaaa
    63661 aaggaataag atgacagaag aaatcaaaaa tctgcaggca caggattatg atgccagtca
    63721 aattcaagtt ttagagggct tagaggctgt tcgtatgcgt ccagggatgt acattggatc
    63781 aacctcaaaa gaaggtcttc accatctagt ctgggaaatt gttgataact caattgacga
    63841 ggccttggca ggattttcca gccatattca agtttttatt gagccagatg attcgattac
    63901 tgttgtggat gatgggcgtg gtatcccagt cgatattcag gaaaaaacag gccgtcctgc
    63961 tgttgagacc gtctttacag tccttcatgc tggaggaaag ttcggcggtg gtggatacaa
    64021 ggtttcaggt ggtcttcacg gggtggggtc gtcagtagtt aatgcccttt ccactcaatt
    64081 agacgttcat gtccataaaa acggtaagat tcattaccaa gaataccgtc gtggtcatgt
    64141 tgttgcagac cttgaggtgg ttggggatac agatagaact ggaacaacgg ttcacttcat
    64201 accggaccca aaaatcttta ctgaaacaac aacctttgat tttgataaat taaataaacg
    64261 gattcaagag ttggcctttt taaatcgcgg tcttcaaatt tctatcactg ataagcgcca
    64321 aggtttggaa caaaccaagc attatcatta tgaaggtggg attgctagtt acgttgaata
    64381 tatcaacgag aacaaggatg taatctttga tacaccaatc tatacagacg gtgagatgga
    64441 tgatatcaca gttgaggtag ccatgcagta cacaacgggt taccatgaaa atgtcatgag
    64501 tttcgccaat aatattcata cacatgaagg tggaacgcat gaacaaggtt tccgtacagc
    64561 cttgacacgt gttatcaacg attatgctcg taaaaataag ttactgaaag acaatgaaga
    64621 caatctaaca ggggaagatg ttcgcgaagg cttaactgca gttatctcag ttaaacaccc
    64681 aaatccacag tttgaaggac aaaccaagac caaattggga aatagcgaag tggtcaagat
    64741 taccaatcgc ctcttcagtg aagccttctc cgatttcctc atggaaaatc cacagattgc
    64801 caaacgtatc gtagaaaaag ggattttagc tgccaaggct cgtgtggctg ccaagcgtgc
    64861 gcgtgaagtc acacgtaaaa aatctggttt ggaaatttcc aaccttccag ggaaactagc
    64921 agactgttct tctaataacc ctgctgaaac agaactcttc atcgtcgaag gagactcagc
    64981 tggtggatca gccaaatctg gtcgtaaccg tgagtttcag gctatccttc caattcgcgg
    65041 taagattttg aacgttgaaa aagcaagtat ggataagatt ctagctaacg aagaaattcg
    65101 tagtcttttc acagccatgg gaacaggatt tggcgcagaa tttgatgttt cgaaagcccg
    65161 ttaccaaaaa ctcgttttga tgaccgatgc cgatgtcgat ggagcccaca ttcgtaccct
    65221 tcttttaacc ttgatttatc gttatatgaa accaatccta gaagctggtt atgtttatat
    65281 tgcccaacca ccaatctatg gtgtcaaggt tggaagcgag attaaagaat atatccagcc
    65341 gggtgcagat caagaaatca aactccaaga agctttagcc cgttatagtg aaggtcgtac
    65401 caaaccgact attcagcgtt ataaggggct aggtgaaatg gacgatcatc agctgtggga
    65461 aacaaccatg gatcccgaac atcgcttgat ggctagagtt tctgtagatg atgctgcaga
    65521 agcagataaa atctttgata tgttgatggg ggatcgagta gagcctcgtc gtgagtttat
    65581 cgaagaaaat gctgtctata gtacacttga tgtctaaaaa atagggagaa gtagttccct
    65641 tggtctaaaa aatatgatat aatcgttacg attgtttcaa gtaaaaaagg agtttgatat
    65701 gtctaatgga caactaattt atttaatggt tgcaattgca gtcattttag ttctggctta
    65761 tgtagtggca atctttctac gtaagcgaaa cgaggggaga ttagaggcgc tagaagaaag
    65821 aaaagaagaa ctatacaatc ttccagtaaa tgatgaagta gaagctgtaa aaaatatgca
    65881 cttgattgga caaagtcaag tggctttccg tgaatggaat caaaaatggg tcgatttatc
    65941 tctcaactct tttgccgata ttgaaaataa tctctttgaa gcagaaggct ataaccattc
    66001 atttcgtttt ctcaaggcca gtcatcaaat tgaccaaatt gagagtcaaa ttactttgat
    66061 tgaagaagat attgcggcaa ttcgcaatgc tttggcagac ttagagaagc aagaatctaa
    66121 aaatagtggt cgtgttcttc atgctttgga tttatttgag gaacttcagc atagagttgc
    66181 tgaaaattca gaacagtatg gtcaagcctt ggatgaaatt gaaaaacaat tagaaaatat
    66241 ccaatctgaa ttttcacaat ttgtaacctt gaattcatcg ggtgaccctg tggaagccgc
    66301 agtgattttg gataatacag aaaatcacat tttggcctta agtcatattg tggatcgtgt
    66361 tccagccttg gttacgacgc tttctacaga attgccagat caattacagg atttggaatc
    66421 cggttatcgt aaactaattg atgctaatta tcattttgtt gaaacggata ttgaagcgcg
    66481 tttccacttg ctttatgaag cattcaagaa aaaccaagag aatattcgtc agttggaatt
    66541 ggataatgcc gaatatgaga atggacaggc acaagaggaa atcaatgcct tgtatgatat
    66601 ttttactcga gaaattgctg ctcagaaagt agtggaaaat ctacttgcaa ctcttccaac
    66661 ttatcttcaa catatgaaag agaataatac tttattggga gaagatattg cacgtttgaa
    66721 caagacctat ttacttcctg agacagctgc aagccatgtt cgtcgtattc agacagaatt
    66781 agagagtttt gaggcagcta ttgttgaggt aacttcaaat caagaagaac caacccaagc
    66841 ttattcagtt cttgaagaaa atcttgagga tttacaaact caactaaaag atattgaaga
    66901 tgagcaaatt tcagttagtg agcgcctgac acaaattgag aaagatgata ttaatgcacg
    66961 tcaaaaggcc aatgtttatg tcaatcgtct ccatactatc aagcgataca tggaaaaacg
    67021 caatctgcca ggtattccac aaactttctt gaagttattc tttacggcaa gcaataatac
    67081 cgaggattta atggttgagt tagaacaaaa aatgattaac attgaatctg ttacccgagt
    67141 tcttgaaatt gcaacgaatg atatggaagc tttagaaacg gaaacttata atattgtaca
    67201 atatgcaact ttgacagagc aactcttaca atattctaac cgctatcgct catttgatga
    67261 acgcattcaa gaagcattta acgaagcttt agatattttt gaaaaagaat ttgattatca
    67321 cgcttcattt gacaagattt ctcaagcatt ggaagtggca gagcctggtg taaccaatcg
    67381 ctttgttacc tcatatgaga aaacacgtga aacgattcgt ttttaataaa agaaaaagat
    67441 tttattgtgt gaggagcaga atcaaatctt tttctatagt tgtggggaga tttacttcat
    67501 tttctcctga gattgagttt ttgcccagcc gatttatcca ccacctcaaa acagtgtttt
    67561 atactcttcg aaaatctctt caaatcacgt cagcgtcgcc ttaccgtact caagtacagc
    67621 ctgaggctct tagtttgctt tttgattttc atttagtatt aaagtgattt cgtcagtctt
    67681 atctgcagct tcaaatctgt actttgagta acttggtaac cgtccaataa cgaagtctat
    67741 t
//
LOCUS       DAVLVP010000002        58698 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.002, whole genome shotgun sequence.
ACCESSION   DAVLVP010000002 DAVLVP010000000
VERSION     DAVLVP010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 58698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58698
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.002"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            41..814
                     /locus_tag="VYN69_000072"
     CDS             41..814
                     /locus_tag="VYN69_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1532433.1"
                     /translation="MLAACSGGAKKEGQAASKKEIIVATNSSPKPFNYEENGELTGYE
                     IEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYQMAVNNISYTKERAEKYLYAAPT
                     VKDPNVLVVKKDDSSIKSLDDVGGKSTEVVQGTTSAKQLEDYNKQHSDNPTILNYTKA
                     NLQQIMGHLSDGQFDYKIFDKITVETVIKNQGLDNLKVIDLPSDQQPYVYPLLAKGQD
                     ELKTFVDKRIQELYKDGTLEKLSKQFFGDTYLPAEADIK"
     gene            855..932
                     /locus_tag="VYN69_000073"
                     /pseudo
     CDS             855..932
                     /locus_tag="VYN69_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000253911.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC protein"
     gene            1036..1866
                     /locus_tag="VYN69_000074"
     CDS             1036..1866
                     /locus_tag="VYN69_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1532434.1"
                     /translation="MKKIVKYSSLAALGLVAAGVLAACSGGAKKEGEAASKKEIIVAT
                     NGSPRPFIYEENGELTGYEIEVVRAIFKDSDKYDVKFEKTEWSGVFAGLDADRYNMAV
                     NNLSYTKERAEKYLYAAPIAQNPNVLVVKKDDSSIKSLDDIGGKSTEVVQATTSAKQL
                     EAYNAEHTDNPTILNYTKADFQQIMVRLSDGQFDYKIFDKIGVETVIKNQGLDNLKVI
                     ELPSDQQPYVYPLLAQGQDELKSFVDKRIKELYKDGTLEKLSKQFFGDTYLPAEADIK
                     "
     gene            2020..2874
                     /locus_tag="VYN69_000075"
     CDS             2020..2874
                     /locus_tag="VYN69_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEV1532435.1"
                     /translation="MKIKKWLGLVALATVAGLALAACGNSEKKADNATTIKIATVNRS
                     GSEEKRWDKIQELVKKDGITLEFTEFTDYSQPNKATADGEVDLNAFQHYNFLNNWNKE
                     NGKDLVAIADTYISPIRLYSGLNGSANKYTKVEDIPANGEIAVPNDATNESRALYLLQ
                     SAGLIKLDVSGTALATVANIKENPKNLKITELDASQTARSLSSVDAAVVNNTFVTEAK
                     LDYKKALFKEQADENSKQWYNIIVAKKDWETSPKADAIKKVIAAYHTDDVKKVIEESS
                     DGLDQPVW"
     gene            2974..4347
                     /locus_tag="VYN69_000076"
     CDS             2974..4347
                     /locus_tag="VYN69_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HEV1532436.1"
                     /translation="MVFPSEQEQIEKFEKDHVAQHYFEVLRTLISKKSVFAQQVGLKE
                     VANYLGEIFKRVGAEVEIDESYTAPFVMAHFKSSRPDAKTLIFYNHYDTVPADGDQVW
                     TEDPFTLSVRNGFMYGRGVDDDKGHITARLSALRKYMQHHDDLPVNISFIMEGAEESA
                     STDLDKYLEKHADKLRGADLLVWEQGTKNALEQLEISGGNKGIVTFDAKVKSADVDIH
                     SSYGGVVESAPWYLLQALQSLRAADGRILVEGLYEEVQEPNEREMALLETYGQRNPEE
                     VSRIYGLELPLLQEERMAFLKRFFFEPALNIEGIQSGYQGQGVKTILPAEASAKLEVR
                     LVPGLEPHDVLEKIRKQLDKNGFDKVELYYTLGEMSYRSDMSAPAILNVIELAKKFYP
                     QGVSVLPTTAGTGPMHTVFDALEVPMVAFGLGNANSRDHGGDENVRIADYYTHIELVE
                     ELIRSYE"
     gene            4340..5401
                     /locus_tag="VYN69_000077"
     CDS             4340..5401
                     /locus_tag="VYN69_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HEV1532437.1"
                     /translation="MSRDIIKLDQIDVTFHQKKRTITAVKDVTIHIQEGDIYGIVGYS
                     GAGKSTLVRVINLLQKPSAGKITIDDDVIFDGKVTLTAEQLRRKRQDIGMIFQHFNLM
                     SQKTAEENVAFALKHSGLSKEEKKAKVAKLLDLVGLADRAENYPSQLSGGQKQRVAIA
                     RALANDPKILISDESTSALDPKTTKQILALLQDLNQKLGLTVVLITHEMQIVKDIANR
                     VAVMQDGHLIEEGSVLEIFSDPKQPLTQDFISTATGIDEAMVKIEKQEIVEHLSENSL
                     LVQLKYAGASTDEPLLNELYKHYQVMANILYGNIEILDGTPVGELVVVLSGEKAALAG
                     AQEAIRQAGVQLKVLKGVQ"
     gene            5403..6095
                     /locus_tag="VYN69_000078"
     CDS             5403..6095
                     /locus_tag="VYN69_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1532438.1"
                     /translation="MESLIQTYLPNVYKMGWAGQAGWGTAIYLTLYMTVLSFIIGGFL
                     GLVAGLFLVLTAPGGVLENKVVFWILDKITSIFRAVPFIILLAILSPLSHLIVKTSIG
                     PNAALVPLSFAVFAFFARQVQVVLAELDGGVIEAAQASGATFWDIVGVYLSEGLPDLI
                     RVTTVTLISLVGETAMAGAVGAGGIGNVAIAYGFNRYNHDVTILATIIIILIIFAIQF
                     LGDFLTKKLSHK"
     gene            complement(6125..6694)
                     /locus_tag="VYN69_000079"
     CDS             complement(6125..6694)
                     /locus_tag="VYN69_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1532439.1"
                     /translation="MKKSILTTLLFAVLYFLCMGIGVLLGNLFDQTGNMFYAPAFTAL
                     VGGSVYMILVAKVPRFGAITTIGLVIALFFLGTKHGAGSFLPGIICGLLADEVAQLGK
                     YKDKTKNFLSFIIFAFSTTGPILLMWIAPKAYMATLLARGKSQEYIDRIMVAPNPGTV
                     LLFIASIVIGALVGALIGQALSKKFAQKI"
     gene            6829..7443
                     /locus_tag="VYN69_000080"
     CDS             6829..7443
                     /locus_tag="VYN69_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000532354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1532440.1"
                     /translation="MGRFLDFVFNRFFLGMIATAFFWLLTLVGGIILGLAPASATLMS
                     LYAEHGYSFREYSLKEAWSLYKQNFVSSNLIFYSFLGVGLVLTYGLYLLVQLPHQTIV
                     HLIATLLNVLVVALIFLAYTVSLKLQVYFALSYRNSLKLSLIGIFMSLAAVAKVLLGT
                     VLLVAIGYYMPALLFFVGIGMWHFFISDMLEPVYEIIHEKLATK"
     gene            7445..9091
                     /locus_tag="VYN69_000081"
     CDS             7445..9091
                     /locus_tag="VYN69_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1532441.1"
                     /translation="MKQFWLHTLLRTYSSVMIIIIASFAILLSYADWDSREKEAQRVA
                     QRVTARTVSEIEYYHRESTQIAQALVENQARIEGIYKYFSLSMPDYFYWQLERKASPY
                     ISVSLYENVDDLYVRNDFVTGVAIAFQDYKEVYVSTKDKRSGEKIRAEDFKPVGNSFA
                     IPVSDPVSDQDLGVIYISLDPAVLYHAIDNTRGHTPMVVTVTSPFDTEIFHIGETVDK
                     ESENWLVGLTSHGYQVQVAVPKNFVLQGTVTSSALIVGLSLLFIVILYLTLRQTFANY
                     QKQVVDLVESIQVIAQGEEGRRIDISEKDQELLLIAETTNDMLDRLEKNIHDIYQLEL
                     SQKDANMRALQAQINPHFMYNTLEFLRMYAVMQSQDELADIIYEFSSLLRNNISDERE
                     TLLKQELEFCRKYSYLCMVRYPKSIAYGFKIDPELENMKIPKFTLQPLVENYFAHGVD
                     HRRTDNVISIKALKQDGFVEILVVDNGRGMSAEKLANIREKLSQRYFEHQASYSDQRQ
                     SIGIVNVHERFVLYFGDRYAITIESAEQAGVQYRITIQDE"
     gene            9103..10389
                     /locus_tag="VYN69_000082"
     CDS             9103..10389
                     /locus_tag="VYN69_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1532442.1"
                     /translation="MYKVLLVDDEYMVTEGLKRLIPFDKWDMEVVATASHADEALEYV
                     QENPVDVIISDVNMPDKTGLDMIREMKEILPDAAYILLSGYQEFDYVKRAMNLSVVDY
                     LVKPVDKVELGNLLEKIAGQLGERGKKSQTLSQELDEAGFVSYLGDKENWWIGLSKEK
                     QGSFTIPYYVLGQDWQIFISGHPLDGLVVTPFEAPYQEHFERWKLNAEKTLFYGSVNL
                     QQSESLFAYYEPIYRVIIQGNLNQIVEELNLLEKVVLENTPRVSITKQLFIQFVMDVF
                     HLFEHLKADDMTDIVKTIHAIQSFDELVSYIKETLISFFGQYRMNENVVSVLEVIGRD
                     YQKELSLKDISKALFINPVYLGQLIKRETDSTFAELLNKQRIKAAQQLLLSTSDSIED
                     ICYAVGYSNLGYFYKVFRKLCGKSPKAYRKQVETIL"
     gene            10441..10974
                     /locus_tag="VYN69_000083"
     CDS             10441..10974
                     /locus_tag="VYN69_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1532443.1"
                     /translation="MKKKPIYLWVLLILSALISVPSLFGIVSPLPSKEALRAAQKQVA
                     GVNAQQLEDQLNYTYRVAEASHSIFNVALIVLSTILVVVAIVFLVRKNLQYANYTYVG
                     YVLLAIIGSIYGYVGLQDAVQLVQDESMRLTVSIGSKAVSIFYIVINVLFLALVFYKM
                     WRQQKALAEEEETEELT"
     gene            11052..11522
                     /gene="nrdI"
                     /locus_tag="VYN69_000084"
     CDS             11052..11522
                     /gene="nrdI"
                     /locus_tag="VYN69_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEV1532444.1"
                     /translation="MKTISLVYISLSGNTESFVTRLKDYLLSQYKGIEVQKIHIKDLV
                     KEGKNFYEMDHPYVAFLPTYLEGGNGVDNGDVEILTTPVGDFIAYGNNASKCFGVVGS
                     GNRNFNNQYCLTAKQYSQRFGFPVLADFEMRGMLEDIKHVAAIIADLYELEKEN"
     gene            complement(11815..13077)
                     /locus_tag="VYN69_000085"
     CDS             complement(11815..13077)
                     /locus_tag="VYN69_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000075801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HEV1532445.1"
                     /translation="MSQAISLNQSTWASKLKAMGPGILMATAAVGGSHIVSSTQAGGS
                     YGWSLLLLVILANVFKYPFFRFGAEYTADTGKTLVEGYAEKGKLYLWIFFILNVFSAM
                     VNTAGVAILCSAIIASAFPMIGLSITQWSLILVAIIWAMLLFGGYKLLDGMVKWIMSA
                     LTIATVLAVIIAAVKHPEYSSDFVEKTPWQMAALPFIVSLLGWMPAPIEISAINSLWS
                     AEKRKTVNFNTEDALFDFNTGYIGTAILAVFFVALGALIQYPTGQAVEAASAKYISQF
                     VGMYASVLGEWSRYLITFIAFLCIFGTVITVIDGYSRVNQESLRLLISQKEDNRKSLN
                     IWMTITAIIGIVIIKFFAGQVSTMLRFAMIGSFLTTPFFALLNYALVTRENKNLPSWL
                     KHLAIAGLIFLFGFAIFFIYALAIGKAG"
     gene            complement(13387..13845)
                     /locus_tag="VYN69_000086"
     CDS             complement(13387..13845)
                     /locus_tag="VYN69_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HEV1532446.1"
                     /translation="MKKQVFHDAATGVLIGLILSILFSLIYAPNTYAPLNPYSLIGQV
                     MDQHQVHGALVLLYCTLIWATIGMLFNFGNRLFSRDWSMLRATLTHFFLMLAGFVPLA
                     TLAGWFPFHWIFYLQLIIEFEIVYLIIWAILYKREAKKVDHINQLLEHRK"
     gene            complement(13842..14282)
                     /locus_tag="VYN69_000087"
     CDS             complement(13842..14282)
                     /locus_tag="VYN69_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1532447.1"
                     /translation="MKVELQISETYEEEKLIVQAPQPTDKVQKVIEFAENLDQREKIK
                     GKSDDQVYLVEIGKIQRFYIENRKVLAETASQTYSIDLRLYQVPKLLPSNFIQISQSE
                     IINIDSISHLKLTPNGLVEIFLKNESFTYSSRRYLKTIKEKLEL"
     gene            14638..16587
                     /gene="mutL"
                     /locus_tag="VYN69_000088"
     CDS             14638..16587
                     /gene="mutL"
                     /locus_tag="VYN69_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEV1532448.1"
                     /translation="MSHIIELPEMLANQIAAGEVIERPASVVKELVENAIDAGSSQII
                     IEIEEAGLKKVQITDNGHGIAHDEVELALRRHATSKIKNQADLFRIRTLGFRGEALPS
                     IASVSVLTLLTAVDGASHGTKLVARGGEVEEVIPATSPVGTKVCVEDLFFNTPARLKY
                     MKSQQAELSHIIDIVNRLGLAHPEISFSLISDGKEMTRTAGTGQLRQAIAGIYGLVSA
                     KKMIEIENSDLDFEISGFVSLPELTRANRNYISLFINGRYIKNFLLNRAILDGFGSKL
                     MVGRFPLAVIHIHIDPYLADVNVHPTKQEVRISKEKELMTLVSEAIANSLKEQTLIPD
                     ALENLAKSTVRNREKVEQTILPLKENTLYYEKTEPSRPSQTEVADYQVELTDEGQDLT
                     LFAKETLDRLTKPAKLHFAERKPANYDQLDHPELDLASIDKAYDKLEREEASSFPELE
                     FFGQMHGTYLFAQGRDGLYIIDQHAAQERVKYEEYRESIGNVDQSQQQLLVPYIFEFP
                     ADDALRLKERMPLLEEVGVFLAEYGENQFILREHPIWMAEEEIESGIYEMCDMLLLTK
                     EVSIKKYRAELAIMMSCKRSIKANHRIDDHSARQLLYQLSQCDNPYNCPHGRPVLVHF
                     TKSDMEKMFRRIQENHTSLRELGKY"
     gene            complement(16912..17379)
                     /locus_tag="VYN69_000089"
     CDS             complement(16912..17379)
                     /locus_tag="VYN69_000089"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HEV1532449.1"
                     /translation="MNTYEGNLVANNIKIGIVVARFNEFITSKLLSGALDNLKRENVN
                     EKDIEVAWVPGAFEIPLIASKMAKSKKYDAIICLGAVIRGNTSHYDYVCSEVSKGIAQ
                     ISLNSEIPVMFGVLTTDTIEQAIERAGTKAGNKGSECAQGAIEMVNLIRTLDA"
     gene            complement(17380..18585)
                     /locus_tag="VYN69_000090"
     CDS             complement(17380..18585)
                     /locus_tag="VYN69_000090"
                     /EC_number="3.5.4.25"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     3,4-dihydroxy-2-butanone-4-phosphate synthase/GTP
                     cyclohydrolase II"
                     /protein_id="HEV1532450.1"
                     /translation="MEYRKIQEALEALQKGRLVLVIDDKDRENEGDLICSAQAATTEN
                     VNFMATYAKGLICMPMSESLANQLMLSPMVENNTDNHKTAFTVSIDYKETTTGISAEE
                     RGLTARMCVAEDITPSDFRRPGHMFPLIAKKGGVLERNGHTEATVDLLKLAGLKECGL
                     CCEIMNHDGKMMRTDDLIQFSKKHNIPLITIKELQEYRKVYDQLVERVSTVNMPTRYG
                     NFKAISYIDKLNGEHHLALIMGNIEDEANVLCRVHSECLTGDVLGSLRCDCGQQFDKA
                     MKMIVENGSGVLLYLRQEGRGIGLINKLKAYHLQDQGMDTLDANLALGFEGDLREYHI
                     GAQMLKDLGLQSLHLLTNNPDKVEQLEKYGITISSRISIEIEANPYDSFYLETKKNRM
                     GHILNMEEK"
     gene            complement(18605..19240)
                     /gene="ribE"
                     /locus_tag="VYN69_000091"
     CDS             complement(18605..19240)
                     /gene="ribE"
                     /locus_tag="VYN69_000091"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HEV1532451.1"
                     /translation="MFTGIIEEIGKVERIQKDSRNCKLSIKASKILTDIHLGDSIAVN
                     GICLTVTHFNHQSFTVDVMNETWSRTALTLLKHGSEVNLERALSVNGRLGGHVVTGHI
                     DGTGKISSIKKDDNAVWYQINTQKEILDLIVEKGSITIDGISLTVAKVSKVNFSVSVI
                     PHTLEQTILKSKQVGSTVNLENDILGKYVQKLMDNSPKSEISKELLYQNGF"
     gene            complement(19225..20325)
                     /gene="ribD"
                     /locus_tag="VYN69_000092"
     CDS             complement(19225..20325)
                     /gene="ribD"
                     /locus_tag="VYN69_000092"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HEV1532452.1"
                     /translation="MSDSKYMKLAIKLAQKGAGYVNPNPMVGAVIVKDNHIIGQGYHE
                     FFGGPHAERNALKNCRESPVGATLYVTLEPCCHFGKTPPCIDAIIDSGITRVVIGSLD
                     CNPIVSGKGVKILEENNLQVTVGILENECLNLIKSFRKYITQHVPYVFMKYAMSMDGK
                     IATKTNQSKWITEEEARKHVHQLRHYVSAIMVGVNTVIQDDPLLTCRLEEGKNPIRII
                     CDTHLRTPLTSKIVKTANDIKTYIATSSEDKNKMKLYQNHGCEILSIKKKGNHIDLSS
                     LMQHLGNMQIDSLVLEGGSLMNWSALEQQIVDELKIYIAPKIFGGSAKFPVGGEGISL
                     PNDAIRLKPYAFSQIGNDYLIESEVIYPCSQE"
     regulatory      complement(20460..20574)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            20731..21324
                     /gene="ruvA"
                     /locus_tag="VYN69_000093"
     CDS             20731..21324
                     /gene="ruvA"
                     /locus_tag="VYN69_000093"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEV1532453.1"
                     /translation="MYAYLKGIITKITAKYIVLETNGIGYILHVANPYAYSGQVNQEA
                     QIYVHQVVREDAHLLYGFRSEDEKKLFLSLISVSGIGPVSALAIIAADDNAGLVQAIE
                     TKNITYLTKFPKIGKKTAQQMVLDLEGKVVVAGDDLPAKVAVQASAENQELEEAMEAM
                     LALGYKATELKKIKKFFEGTTDTAENYIKSALKMLVK"
     gene            21334..21897
                     /locus_tag="VYN69_000094"
     CDS             21334..21897
                     /locus_tag="VYN69_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEV1532454.1"
                     /translation="MTKRCSWVKMTNPLYIAYHDEEWGQPLHDDQALFELLCMETYQA
                     GLSWETVLNKRQAFREAFHSYQIHSVAEMTDTELEAMLENPAIIRNRAKIFATRANAQ
                     AFLQLQAEYGSFDAYLWSFVEGKIVVNDVPDYRQAPAKTPLSEKLAKDLKKRGFKFTG
                     PVAVLSFLQAAGLVDDHENDCEWKGLK"
     gene            21894..22571
                     /locus_tag="VYN69_000095"
     CDS             21894..22571
                     /locus_tag="VYN69_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000987430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1532455.1"
                     /translation="MMSNKNKGILIFAILYTVLFVFDGVKLLASLMPSVIANYLVYVV
                     LALYGSFLFKDRLIQQWKEIRKTKRKFFFGVLTGWLFLILMTVVFEFVSEMLKQFVRL
                     DGQGLNQSNIQSTFQEQPLLIAVFACVIGPLVEELFFRQVLLHYLQERLPGLLSIILV
                     GLVFALTHMHSLALSEWIGAVGYLGGGLAFSIIYVKEKENIYYPLLVHMLSNSLSLII
                     LAISIVK"
     gene            complement(22726..23757)
                     /locus_tag="VYN69_000096"
     CDS             complement(22726..23757)
                     /locus_tag="VYN69_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000255096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEV1532456.1"
                     /translation="MVSTIGIVSLSSGIIGEDFVKHEVDLGIQRLKDLGLNPIFLPHS
                     LKGLDFIKDHPEARAEDLIHAFSDDSIDMILCAIGGDDTYRLLPYLFENDQLQKVIKQ
                     KIFLGFSDTTMNHLMLHKLGIKTFYGQSFLADICELDKEMLAYSLHYFKELIETGRIS
                     EIRPSDVWYEERTDFSPTALGTPRVSHTNTGFDLLQGSAQFEGKILGGCLESLYDIFD
                     NSRYADSTELCQKYKLFPDLSDWEGKILLLETREEKPKPEDFKKMLLTLKDTGIFAVI
                     NGLLVGKPMDETFHDDYKEALLDIIDNNIPIVYNLNVGHATPRAIVPFGVHAHVDAQE
                     QVIRFDYNK"
     gene            complement(24012..24695)
                     /locus_tag="VYN69_000097"
     CDS             complement(24012..24695)
                     /locus_tag="VYN69_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEV1532457.1"
                     /translation="MSQIDLQKLTKKNQEFVHIATQQFIKDGKTDAEIQTIFEEVIPQ
                     ILEEQSKGTTARSLYGAPTHWAHSFTVKEQYEKEHPKENDDPKLMIMDSALFITSLFA
                     LVSALTTFFAADQAFGYGLITLLLVGLVGGFAFYLMYYFVYQYYGPDMDRSQRPPFWK
                     SVLVILASMFLWLLVFFATSFLPASLNPVLDPLPLAIIGAALLALRFYLKKRLNIRSA
                     SAGPTRYQE"
     gene            complement(24707..25651)
                     /locus_tag="VYN69_000098"
     CDS             complement(24707..25651)
                     /locus_tag="VYN69_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1532458.1"
                     /translation="MKQVFLSTTTEFKEIDTLEPGTWINLVNPTQNESLEIANTFDID
                     IADLRAPLDAEEMSRITIEDEYTLIIVDVPVTEERNNRTYYVTIPLGIIITEETIITT
                     CLEPLPVLDVFINRRLRNFYTFMRSRFIFQILYRNAELYLTALRSIDRKSEQIESQLH
                     QSTRNEELIELMELEKTIVYFKASLKTNERVIKKLTSSTSNIKKYLEDEDLLEDTLIE
                     TQQAIEMADIYGNVLHSMTETFASIISNNQNNIMKTLALVTIVMSIPTMVFSAYGMNF
                     KDNEIPLNGEPNAFWLIVFIAFAMSVSLTLYLIHKKWF"
     gene            25783..28614
                     /gene="uvrA"
                     /locus_tag="VYN69_000099"
     CDS             25783..28614
                     /gene="uvrA"
                     /locus_tag="VYN69_000099"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEV1532459.1"
                     /translation="MQDKIVIHGARAHNLKNIDVEIPRDKLVVVTGLSGSGKSSLAFD
                     TLYAEGQRRYVESLSAYARQFLGNMEKPDVDAIDGLSPAISIDQKTTSKNPRSTVGTT
                     TEINDYLRLLYARVGTPYCINGHGAINASSVEQIVDKVLELPEGQRLQILAPVIRKKK
                     GQHKSVIEKVQKDGYVRVRVDGEVYDVTEVPELSKSKQHNIDVVVDRIVIKEGIRSRL
                     FDSIEAALRIAEGYVIIDTMDDSELLFSEHYACPVCGFTVPELEPRLFSFNAPFGSCS
                     ECDGLGIKLEVDTDLVVPDASKTLREGALAPWNPISSNYYPNMLEQAMKVFGVAMDKP
                     FEDLSEEDKNLILYGSDGKEFHFHYENEFGGVRDIDIPFEGVINNIKRRYHETNSDYT
                     RTQMRLYMNELTCGTCQGYRLNDQALSVRVGGQQGPHIGEISDLSIADHLDLVSQLTL
                     SENEAIIARPILKEIKDRLTFLNNVGLNYLTLSRSAGTLSGGESQRIRLATQIGSNLS
                     GVLYILDEPSIGLHQRDNDRLIASLKKMRDLGNTLIVVEHDEDTMREADYLIDVGPGA
                     GVFGGEIVAAGTPKQVARNSKSITGQYLSGKRVIPVPEERRVGNGRFIEVIGARENNL
                     QNVTARFPLGKFIAVTGVSGSGKSTLINSILKKAIAQKLNRNSDKPGKFKTITGIEHV
                     DRLIDIDQSPIGRTPRSNPATYTGVFDDIRDLFAQTNEAKIRGYKKGRFSFNVKGGRC
                     EACSGDGIIKIEMHFLPDVYVACEVCHGTRYNSETLEVHYKEKNISQVLDMTVNDAVE
                     FFQHIPKIQRKLQTIKDVGLGYVTLGQPATTLSGGEAQRMKLASELHKRSTGKSFYIL
                     DEPTTGLHTEDIARLLKVLARFVDDGNTVLVIEHNLDVIKTADHIIDLGPEGGVGGGT
                     IIVTGTPEEVAANEASYTGHYLKGKLHHE"
     gene            28607..29668
                     /locus_tag="VYN69_000100"
     CDS             28607..29668
                     /locus_tag="VYN69_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1532460.1"
                     /translation="MNKRVQAFLAKMQEKELDGIIINNLKNVYYLTGFWGSNGTVFIS
                     RDRQVLVTDSRYIIAAKQETSGFEIVADRDELAVIAGIVKDMGLTRIGFEDEISVSYY
                     HRMQAAFAGLDLLPQTQFVEGLRMIKDEAEIAAIRKACSISDQAFRDALDFIKPGKTE
                     IEIANFLDFRMRELGASGLSFDTILASGINSSKPHAHPMHKPVELGEAITMDFGCLYD
                     HYVSDMTRTIYLGHVSDEQAEIYNTVLKANQALIDQAKAGLGFRDFDKIPRDIIIEAG
                     YGDYFTHGIGHGIGLDIHEEPYFSQTSTETIKTGMALTDEPGIYIEGKYGVRIEDDIL
                     ITETGCELLTLAPKELIVI"
     gene            29800..30198
                     /gene="spx"
                     /locus_tag="VYN69_000101"
     CDS             29800..30198
                     /gene="spx"
                     /locus_tag="VYN69_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017768833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEV1532461.1"
                     /translation="MIKIYTVSSCTSCKKAKTWLNAHQLSYKEQNLGKEGITREELLD
                     ILTKTDNGIASIVSSKNRYAKALGVDIEDLSVNEVLNLIMETPRILKSPILVDEKRLQ
                     VGYKEDDIRAFLPRSVRNVENAEARLRAAL"
     gene            30264..30833
                     /locus_tag="VYN69_000102"
     CDS             30264..30833
                     /locus_tag="VYN69_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEV1532462.1"
                     /translation="MKKIVLVSLAFLFVLVGCGQKKETGPATKTEKDTLQSALPVIEN
                     AEKNTVVTKTLVLPKSDDGSQQTQTITYKDKTFLSLTIQQKRPVSDELKTYIDQHGVE
                     ETQKALLEAEEKDKSIIEARKLAGFKLETKLLSATELQTTTSFDFQVLDVKKASQLEH
                     LKNIGLENLLKNEPSNYISDRLANGATEQ"
     gene            30920..31186
                     /locus_tag="VYN69_000103"
     CDS             30920..31186
                     /locus_tag="VYN69_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEV1532463.1"
                     /translation="MGFTEETVRFKLDDSNKKEISETLTDVYASLNDKGYNPINQIVG
                     YVLSGDPAYVPRYNNARNQIRKYERDEIVEELVRYYLKGQGVDL"
     gene            31190..31609
                     /gene="ruvX"
                     /locus_tag="VYN69_000104"
     CDS             31190..31609
                     /gene="ruvX"
                     /locus_tag="VYN69_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002939356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEV1532464.1"
                     /translation="MRIMGLDVGSKTVGVAISDPLGFTAQGLEIIQINEEQGQFGFDR
                     VKELVDTYKVERFVVGLPKNMNNTSGPRVEASQAYGAKLEEFFGLPVDYQDERLTTVA
                     AERMLIEQADISRNKRKKVIDKLAAQLILQNYLDRKF"
     gene            31625..31930
                     /locus_tag="VYN69_000105"
     CDS             31625..31930
                     /locus_tag="VYN69_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEV1532465.1"
                     /translation="MSHDHNHDHEERELITLVDEQGNETLFEILLTIDGKEEFGKNYV
                     LLVPVNAEEDEDGQVEIQAYSFIENEDGTEGELQPIPEDSEDEWNMIEEVFNSFMEE"
     gene            32236..33486
                     /locus_tag="VYN69_000106"
     CDS             32236..33486
                     /locus_tag="VYN69_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1532466.1"
                     /translation="MFEVEEWLHSRIGLNFRSGLGRMQQAVDLLGNPEQSYPIIHVTG
                     TNGKGSTIAFMRELFMGHGKKVATFTSPHIVSINDRICINGQPIADADFIRLTDQVKE
                     MEKTLLQTPAQLSFFELLTLVAFLYFREQEVDLVLLEVGIGGLLDTTNVVTGELAVIT
                     SIGLDHQETLGDSLEAIAEQKAGIFKAGKKAVIAKLPPEARLVCQKKAESLAVNLYQA
                     GQDFLMLNGDFSSSLLNLSQLNIGLEGVYQQENAALALQTFLLFMGEGKEAVDEQAVR
                     KALEQTHWAGRLERIRPQIYLDGAHNLPALTRLVEFIKEKEQEGYRPQILFGALKRKD
                     YQGMLGYLTEKLPQVELKVTGFDYQGALDERDVTGYDIVSSYREFISDFEERADAQDL
                     LFVTGSLYFISEVRGYLLDREQIN"
     gene            33483..34028
                     /locus_tag="VYN69_000107"
     CDS             33483..34028
                     /locus_tag="VYN69_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001816192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin/fatty acid-binding family protein"
                     /protein_id="HEV1532467.1"
                     /translation="MTPLFELVILGELLVRENFSKLVAERKFIMKLKRFTLSLASLAS
                     FSLLVACSQRVQQVQQPVAQQQVQQPAQQNTNTANAGGNQNQAAPVQNQPVAQPTDID
                     GTYTGRDDGDRITLVVTGTTGTWTELESDGDQKVKQVTFDSANQRMIIGDDVKIYTVN
                     GNQIVVDDMDRDPSDQIVLTK"
     gene            34174..35706
                     /gene="cls"
                     /locus_tag="VYN69_000108"
     CDS             34174..35706
                     /gene="cls"
                     /locus_tag="VYN69_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000877342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEV1532468.1"
                     /translation="MKYRKFQLLMSKYGFSLSIMLLELCLVFGLFLYLGRMAPILWIT
                     VLILLSIITIISIVNRNTTPENKVTWLLVAFVPVFGPLLYLMFGERRLSKKEIKQLKK
                     LGSMHFQEANSQLLKEKLKESDKAAYGVIKSLLSMDTNADIYDQTASTFFPNGEAMWK
                     KMVEDLKKAEKFIFLEYYIIEEGLMWNRILDILEQKVAQGVEVKMLYDDIGCMATLTG
                     DYAHRLRQLGIEAHKFNKVIPRLTVAYNNRDHRKILIVDGQIAYTGGVNLADEYINHV
                     ERFGYWKDSGIRLDGLAVKALTRLFLTTWYINRGEISDFDQYHLENHSIPSDGLTIPY
                     GSGPKPIFRAQVGKKVYQSLINQATESVYITTPYLIIDYDLTETIKNAAMRGVDVRII
                     TPCIPDKKFIQLVTRGAYPDLLSAGVRIYEYSPGFIHSKQMLVDEDFAVVGTINLDYR
                     SLVHHYENAVLLYKTPSIREIARDFRNIFADSQEVYPHSIKTSWYQKLVKEIAQLFAP
                     IL"
     gene            35964..37502
                     /locus_tag="VYN69_000109"
     CDS             35964..37502
                     /locus_tag="VYN69_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1532469.1"
                     /translation="MSVYGRVEEVHKENREPLEYQIEQESHHRESSRLPLVKILLWST
                     LVTGITLGVPLLLDLMSAQEVQDFYAGWALHQTGKIYSDYYGSQGLLYYLLTYVSQGG
                     FFFAIFEWLALVAGGFFLFRSADTLTEQGDQAGHLVTIFYMLVTGLAFGGGYATLLAL
                     PFLFAAFSLVAAYLSNPSRDKGFVRIGLALAGGFFFAPLSSLLFIAVVSLGLLVFNLG
                     HRRFAHGFYQFLAVALGFSLVFYPTAYYSAATGSFGDAISGIRYPIDSIRFDFTSKIL
                     ENMFFYGLLSLGLGFVVCIFLGLFQSKPFKLYVISVPASLVVILGLILLFFSQEPLHA
                     SYLMVVFPVFLLLLVTNIKSQQRGRSARRSRRETPVSLWSRFFKGNLYLLVFGFVYLL
                     SVPFLMKFVLYPVPYQERNRLADLVKEETNTEDAIYAWDDTATLYRKSERLSPSAILS
                     PLHYTATEENRNKLLNDLKEKQPKVIVVNDKVVVWSEVETLLKENYQQVKTDYSEFKV
                     YKIK"
     gene            37622..39829
                     /gene="nrdD"
                     /locus_tag="VYN69_000110"
     CDS             37622..39829
                     /gene="nrdD"
                     /locus_tag="VYN69_000110"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004239081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEV1532470.1"
                     /translation="MIALEEKITILPTLFVEKRDGRRVVFDVDKIDKALHKAADKVMD
                     VTPLVEKRLNDLTERIITEIHSRFPQGIKIYEIQNIVEHELLEAKEYALAEEYITYRT
                     QRDFERSKATDINFSIHKLLNKDQTVVNENANKDSDVFNTQRDLTAGIVGKSIGLQML
                     PKHVANAHQKGDIHYHDLDYSPYTPMTNCCLIDFKGMLENGFKIGNAEVESPKSIQTA
                     TAQISQIIANVASSQYGGCSADRIDEILAPYAEKNYQKHLKDAEEWVLPEKREDYAWK
                     KTQKDIYDAMQSLEYEINTLFTSNGQTPFTSLGFGLGTSRFEREIQKAILNIRIKGLG
                     SEHRTAIFPKLIFTLKRGLNLEEGTPNYDIKQLALECATKRMYPDVLSYDKIVDLTGS
                     FKVPMGCRSFLQGWKDENGVEVNSGRMNLGVVTVNLPRIALESEGDMNKFWEIFNERM
                     NIAEDALVYRVERTKEATPANAPILYQYGAFGHRLGKEESVDQLFKNRRATVSLGYIG
                     LYEVATVFFGNSWESNPDAKEFTLDIIHDMKRRVEEWSDQYGYHFSIYSTPSESLTDR
                     FCRLDIDKFGSIPDITDKEYYTNSFHYDVRKNPTPFEKLDFEKVYPEAGASGGFIHYC
                     EYPVLQQNPKALEAVWDYAYDRVGYLGTNTPIDRCYKCDFEGDFEPTERGFACPNCGN
                     SDPKTVDVVKRTCGYLGNPQARPMVNGRHKEIAARVKHMNGSTIKIAGHQVTN"
     gene            39841..39981
                     /locus_tag="VYN69_000111"
     CDS             39841..39981
                     /locus_tag="VYN69_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532471.1"
                     /translation="MGKYQLDDKGRAQVTRYHEKHSKGGAGKKERLLSFREQFLNKNK
                     KK"
     gene            40025..40531
                     /locus_tag="VYN69_000112"
     CDS             40025..40531
                     /locus_tag="VYN69_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1532472.1"
                     /translation="MELRRPRLADKKAVLDMMTEFEKFQSPHDGGFWDTENFVYEDWL
                     ESNQEQEMGINLPEGWVPAIQLVAFSEKGQAVGFLNLRLRLSNFLLEEGGHIGYSIRP
                     SERGKGYAKETLRQGLQVAKEKNIKKALVTCSVNNPASRAVILANGGIFEDARNGVER
                     YWIEVANE"
     gene            40524..41114
                     /gene="nrdG"
                     /locus_tag="VYN69_000113"
     CDS             40524..41114
                     /gene="nrdG"
                     /locus_tag="VYN69_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001064213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEV1532473.1"
                     /translation="MNNPKPQEWKSEELSQGRIIDYKAFNFVDGEGVRNSLYVAGCMF
                     HCEGCYNVATWSFNAGIPYTAELEEQIMADLAQPYVQGLTLLGGEPFLNTGILLPLVK
                     RIRKELPDKDIWSWTGYTWEEMMLETPDKLELLSLIDILVDGRYDRTKRNLMLQFRGS
                     SNQRIIDVQKSLKSGQVVIWDKLNDGKESYEQVKRE"
     gene            41111..41728
                     /locus_tag="VYN69_000114"
     CDS             41111..41728
                     /locus_tag="VYN69_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HEV1532474.1"
                     /translation="MKKKDLVDQLVSEIETGKVRTLGIYGHGASGKSTFAQELYQALD
                     STTVNLLETDPYITSGRYLVVPKDAPNQKVTASLPVAHELESLQRDILALQAGMDVLT
                     IEEPWKASEVLSGAKPILIVEGMSVGFLPKELFEKTICFYTDEETELKRRLARDTTVR
                     NRDASFILASHQMRREQYLRYYKETESKADILVDQSEDKFDVKRT"
     gene            41995..42303
                     /gene="rpsJ"
                     /locus_tag="VYN69_000115"
     CDS             41995..42303
                     /gene="rpsJ"
                     /locus_tag="VYN69_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEV1532475.1"
                     /translation="MANKKIRIRLKAYEHRTLDTAAAKIVESATRTGAQVAGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTQKTVDALMKLDLPSGVNVEIKL
                     "
     gene            42519..43145
                     /gene="rplC"
                     /locus_tag="VYN69_000116"
     CDS             42519..43145
                     /gene="rplC"
                     /locus_tag="VYN69_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002986659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEV1532476.1"
                     /translation="MTKGILGKKVGMTQIFTEAGELIPVTVIEATPNVVLQVKTVETD
                     GYNAIQVGFDDKREVLSNKPAKGHVAKANTAPKRFIREFKNVEGLEVGAEITVETFAA
                     GDVVDVTGTSKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKGKNLAG
                     RMGGDRVTIQNLEVVQVVPEKNVILIKGNVPGAKKSLITIKSAVKAGK"
     gene            43170..43793
                     /gene="rplD"
                     /locus_tag="VYN69_000117"
     CDS             43170..43793
                     /gene="rplD"
                     /locus_tag="VYN69_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEV1532477.1"
                     /translation="MANVTLFDQTGKEAGQVVLSDAVFGIEPNESVVFDVIISQRASL
                     RQGTHAVKNRSAVSGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYGYKLP
                     QKVRRLALKSVYSEKVAENKFVAVDALSFTAPKTAEFAKVLAALSIDSKVLVILEEGN
                     EFAALSARNLPNVKVATATTASVLDIANSDKLLVTQAAISKIEEVLA"
     gene            43793..44089
                     /locus_tag="VYN69_000118"
     CDS             43793..44089
                     /locus_tag="VYN69_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEV1532478.1"
                     /translation="MNLYDVIKKPVITESSMAQLEAGKYVFEVDTRAHKLLIKQAVEA
                     AFEGVKVANVNTINVKPKAKRVGRYTGFTNKTKKAIITLTADSKAIELFAAEAE"
     gene            44107..44940
                     /gene="rplB"
                     /locus_tag="VYN69_000119"
     CDS             44107..44940
                     /gene="rplB"
                     /locus_tag="VYN69_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019790357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEV1532479.1"
                     /translation="MGIRVYKPTTNGRRNMTSLDFAEITTSTPEKSLLVALKSKAGRN
                     NNGRITVRHQGGGHKRFYRLVDFKRNKDNVEAVVKTIEYDPNRSANIALVHYTDGVKA
                     YIIAPKGLEVGQRIVSGPEADIKVGNALPLANIPVGTLIHNIELKPGRGGELVRAAGA
                     SAQVLGSEGKYVLVRLQSGEVRMILGTCRATVGVVGNEQHGLVNLGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGKAPVGRKAPSTPWGKPALGLKTRNKKAKSDKLIVRRRNE
                     K"
     gene            45044..45325
                     /gene="rpsS"
                     /locus_tag="VYN69_000120"
     CDS             45044..45325
                     /gene="rpsS"
                     /locus_tag="VYN69_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEV1532480.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQANDEKKKVIKTWSRRSTIFPSF
                     IGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYKGHAADDKKTRRK"
     gene            45337..45681
                     /gene="rplV"
                     /locus_tag="VYN69_000121"
     CDS             45337..45681
                     /gene="rplV"
                     /locus_tag="VYN69_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEV1532481.1"
                     /translation="MAEITSAKAMARTVRVSPRKSRLVLDNIRGKSVADAIAILTFTP
                     NKAAEIILKVLNSAVANAENNFGLDKANLVVSEAFANEGPTMKRFRPRAKGSASPINK
                     RTAHITVAVAEK"
     gene            45694..46347
                     /gene="rpsC"
                     /locus_tag="VYN69_000122"
     CDS             45694..46347
                     /gene="rpsC"
                     /locus_tag="VYN69_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEV1532482.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYADYLHEDLAIRKFVQK
                     ELADAAVSTIEIERAVNKVNVSLHTAKPGMVIGKGGANVDALRAKLNKLTGKQVHINI
                     IEIKQPDLDAHLVGEGIARQLEQRVAFRRAQKQAIQRAMRAGAKGIKTQVSGRLNGAD
                     IARAEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEVLPARKNTKGGK"
     gene            46351..46764
                     /gene="rplP"
                     /locus_tag="VYN69_000123"
     CDS             46351..46764
                     /gene="rplP"
                     /locus_tag="VYN69_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003029477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEV1532483.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQATTSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKV
                     MFEIAGVSEEIAREALRLASHKLPVKCKFVKREAE"
     gene            46774..46980
                     /locus_tag="VYN69_000124"
     CDS             46774..46980
                     /locus_tag="VYN69_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEV1532484.1"
                     /translation="MKLNEVKEFVKELRGLSQEELAKRENELKKELFELRFQAATGQL
                     EQTARLKEVKKQIARIKTVQSEAK"
     gene            47005..47265
                     /gene="rpsQ"
                     /locus_tag="VYN69_000125"
     CDS             47005..47265
                     /gene="rpsQ"
                     /locus_tag="VYN69_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEV1532485.1"
                     /translation="MERNNRKVLVGRVVSDKMDKTITVVVETKRNHPVYGKRINYSKK
                     YKAHDENNVAKEGDIVRIMETRPLSATKRFRLVEVVEEAVII"
     gene            47291..47659
                     /gene="rplN"
                     /locus_tag="VYN69_000126"
     CDS             47291..47659
                     /gene="rplN"
                     /locus_tag="VYN69_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEV1532486.1"
                     /translation="MIQTETRLKVADNSGAREILTIKVLGGSGRKFANIGDVIVASVK
                     QATPGGAVKKGDVVKAVIVRTKSGARRADGSYIKFDENAAVIIREDKTPRGTRIFGPV
                     ARELREGGFMKIVSLAPEVL"
     gene            47737..48042
                     /gene="rplX"
                     /locus_tag="VYN69_000127"
     CDS             47737..48042
                     /gene="rplX"
                     /locus_tag="VYN69_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011921687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEV1532487.1"
                     /translation="MFVKKGDKVRVIAGKDKGTEAVVLTALPKVNKVIVEGVNIVKKH
                     QRPTNELPQGGIIEKEAAIHVSNVQVLDKNGVAGRVGYKFVDGKKVRYNKKSGEVLD"
     gene            48066..48608
                     /gene="rplE"
                     /locus_tag="VYN69_000128"
     CDS             48066..48608
                     /gene="rplE"
                     /locus_tag="VYN69_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEV1532488.1"
                     /translation="MANRLKEKYLNEVVPALTEQFNYSSVMAVPKVDKIVLNMGVGEA
                     VSNAKSLEKAAEELALISGQKPLITKAKKSIAGFRLREGVAIGAKVTLRGERMYEFLD
                     KLVSVSLPRVRDFHGVPTKSFDGRGNYTLGVKEQLIFPEINFDDVDKTRGLDIVIVTT
                     ANTDEESRALLTGLGMPFAK"
     gene            48626..48895
                     /gene="rpsN"
                     /locus_tag="VYN69_000129"
     CDS             48626..48895
                     /gene="rpsN"
                     /locus_tag="VYN69_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006738440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEV1532489.1"
                     /translation="MAKKSMVAREAKRQKIVDRYAEKRAALKAAGDYEGLSKLPRNAS
                     PTRLHNRCRVTGRPHSVYRKFGLSRIAFRELAHKGQIPGVTKASW"
     gene            49180..49578
                     /gene="rpsH"
                     /locus_tag="VYN69_000130"
     CDS             49180..49578
                     /gene="rpsH"
                     /locus_tag="VYN69_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEV1532490.1"
                     /translation="MVMTDPIADFLTRIRNANQAKHEVLEVPASNIKKGIAEILKREG
                     FVKNVEIIEDDKQGVIRVFLKYGPNGEKVITNLKRVSKPGLRVYKKREDLPKVLNGLG
                     IAILSTSEGLLTDKEARQKNVGGEVIAYVW"
     gene            49770..50306
                     /gene="rplF"
                     /locus_tag="VYN69_000131"
     CDS             49770..50306
                     /gene="rplF"
                     /locus_tag="VYN69_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEV1532491.1"
                     /translation="MSRIGNKVIVLPAGVELANNDNVVTVKGPKGELTREFSKDIEIR
                     VEGTEITLHRPNDSKEMKTIHGTTRALLNNMVVGVSEGFKKELEMRGVGYRAQLQGSK
                     LVLAVGKSHPDEVEAPEGITFELPNPTTIVVSGISKEVVGQTAAYVRSLRSPEPYKGK
                     GIRYVGEFVRRKEGKTGK"
     gene            50390..50746
                     /gene="rplR"
                     /locus_tag="VYN69_000132"
     CDS             50390..50746
                     /gene="rplR"
                     /locus_tag="VYN69_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEV1532492.1"
                     /translation="MISKPDKNKLRQKRHRRVRGKLSGTADRPRLNVFRSNTGIYAQV
                     IDDVAGVTLASASTLDKEVSKGTKTEQAVAVGKLVAERANAKGISEVVFDRGGYLYHG
                     RVKALADAARENGLKF"
     gene            50764..51258
                     /gene="rpsE"
                     /locus_tag="VYN69_000133"
     CDS             50764..51258
                     /gene="rpsE"
                     /locus_tag="VYN69_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEV1532493.1"
                     /translation="MAFKDNAVELEERVVAVNRVTKVVKGGRRLRFAALVVVGDHNGR
                     VGFGTGKAQEVPEAIRKAVDDAKKNLIEVPMVGTTIPHEVLSEFGGAKVLLKPAVEGS
                     GVAAGGAVRAVVELAGVADITSKSLGSNTPINIVRATVEGLKQLKRAEEIAALRGISV
                     SDLA"
     gene            51230..51454
                     /gene="rpmD"
                     /locus_tag="VYN69_000134"
     CDS             51230..51454
                     /gene="rpmD"
                     /locus_tag="VYN69_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEV1532494.1"
                     /translation="MVFQFLIWHKKGDKMAQIKITLTKSPIGRIPSQRKTVVALGLGK
                     LNSSVIKEDNAAIRGMITAVSHLVTVEEVN"
     gene            51599..52039
                     /gene="rplO"
                     /locus_tag="VYN69_000135"
     CDS             51599..52039
                     /gene="rplO"
                     /locus_tag="VYN69_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004225266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEV1532495.1"
                     /translation="MKLHELKPAEGSRKVRNRVGRGTSSGNGKTSGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNINAKEYAIVNLDQLNVFEDGAEVTPVVLIEAGIV
                     KAEKSGIKILGNGELTKKLTVKAAKFSKSAEEAITAKGGSVEVI"
     gene            52052..53362
                     /gene="secY"
                     /locus_tag="VYN69_000136"
     CDS             52052..53362
                     /gene="secY"
                     /locus_tag="VYN69_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEV1532496.1"
                     /translation="MFFKLLREALKVKQVRSKILFTIFIVLVFRIGTSITVPGVNANS
                     LNALSGLSFLNMLSLVSGNALKNFSIFALGVSPYITASIVVQLLQMDILPKFVEWGKQ
                     GEVGRRKLNQATRYIALVLAFVQSIGITAGFNTLAGAQLIKTALTPQVFLTIGIILTA
                     GSMIVTWLGEQITDKGYGNGVSMIIFAGIVSSIPEMIQGIYVDYFVNVPSSRITSSII
                     FVIILIITVLLIIYFTTYVQQAEYKIPIQYTKVAQGAPSSSYLPLKVNPAGVIPVIFA
                     SSITAAPAAILQFLSATGHDWAWVRVAQEMLATTSPTGIAMYALLIILFTFFYTFVQI
                     NPEKAAESLQKSGAYIHGVRPGKGTEEYMSKLLRRLATVGSLFLGVISILPIAAKDVF
                     GLSDVVAFGGTSLLIIISTGIEGIKQLEGYLLKRKYVGFMDRTE"
     gene            53513..54151
                     /locus_tag="VYN69_000137"
     CDS             53513..54151
                     /locus_tag="VYN69_000137"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEV1532497.1"
                     /translation="MNLLIMGLPGAGKGTQAAKIVEQFHVAHISTGDMFRAAMANQTE
                     MGVLAKSYIDKGELVPDEVTNGIVKERLSQDDIKETGFLLDGYPRTIEQAHALDKTLA
                     ELGIELEGIINIEVNPDSLLERLSGRIIHRVTGETFHKVFNPPVDYKEEDYYQREDDK
                     PETVKRRLDVNIAQGEPIIAHYRAKGLVHDIEGNQDINDVFSDIEKVLTNLK"
     gene            54268..54486
                     /gene="infA"
                     /locus_tag="VYN69_000138"
     CDS             54268..54486
                     /gene="infA"
                     /locus_tag="VYN69_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEV1532498.1"
                     /translation="MAKDDVIEVEGKVVDTMPNAMFTVELENGHQILATVSGKIRKNY
                     IRILAGDRVTVEMSPYDLTRGRITYRFK"
     gene            54511..54627
                     /gene="rpmJ"
                     /locus_tag="VYN69_000139"
     CDS             54511..54627
                     /gene="rpmJ"
                     /locus_tag="VYN69_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEV1532499.1"
                     /translation="MKVRPSVKPICEYCKVIRRNGRVMVICPANPKHKQRQG"
     gene            54645..55010
                     /gene="rpsM"
                     /locus_tag="VYN69_000140"
     CDS             54645..55010
                     /gene="rpsM"
                     /locus_tag="VYN69_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEV1532500.1"
                     /translation="MARIAGVDIPNDKRVVISLTYVYGIGLATSKKILAAAGISEDVR
                     VRDLTSDQEDAIRREVDAIKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGKAVAIAGKKK"
     gene            55028..55411
                     /gene="rpsK"
                     /locus_tag="VYN69_000141"
     CDS             55028..55411
                     /gene="rpsK"
                     /locus_tag="VYN69_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003130556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEV1532501.1"
                     /translation="MAKPTRKRRVKKNIESGIAHIHATFNNTIVMITDVHGNAIAWSS
                     AGALGFKGSRKSTPFAAQMASEAAAKSAQEHGLKSVEVTVKGPGSGRESAIRALAAAG
                     LEVTAIRDVTPVPHNGARPPKRRRV"
     gene            55454..56389
                     /locus_tag="VYN69_000142"
     CDS             55454..56389
                     /locus_tag="VYN69_000142"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEV1532502.1"
                     /translation="MIEFEKPNITKIDENKDYGKFVIEPLERGYGTTLGNSLRRVLLA
                     SLPGAAVTSINIDGVLHEFDTVPGVREDVMQIILNIKGIAVKSYVEDEKIIELDVEGP
                     AEVTAGDILTDSDIEIVNPDHYLFTIGEGSSLKATMTVNSGRGYVPADENKKDNAPVG
                     TLAVDSIYTPVTKVNYQVEPARVGSNDGFDKLTLEILTNGTIIPEDALGLSARILTEH
                     LDLFTNLTEIAKSTEVMKEADTESDDRILDRTIEELDLSVRSYNCLKRAGINTVHDLT
                     EKSEAEMMKVRNLGRKSLEEVKLKLIDLGLGLKDK"
     gene            56401..56787
                     /gene="rplQ"
                     /locus_tag="VYN69_000143"
     CDS             56401..56787
                     /gene="rplQ"
                     /locus_tag="VYN69_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEV1532503.1"
                     /translation="MAYRKLGRTSSQRKAMLRDLTTDLLINESIVTTEARAKEIRKTV
                     EKMITLGKRGDLHARRQAAAFVRNEIASENYDEATDKYTSTTALQKLFSEIAPRYAER
                     NGGYTRILKTEPRRGDAAPMAIIELV"
     gene            57052..57318
                     /locus_tag="VYN69_000144"
     CDS             57052..57318
                     /locus_tag="VYN69_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002905356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HEV1532504.1"
                     /translation="MKAIITVVGKDKSGIVAGVSGKIAELGLNIDDISQTVLDEYFTM
                     MAVVSSDEKQDFTYLRNEFEAFGQTLNVKINIQSAAIFEAMYNI"
     gene            57328..58665
                     /locus_tag="VYN69_000145"
     CDS             57328..58665
                     /locus_tag="VYN69_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PFL family protein"
                     /protein_id="HEV1532505.1"
                     /translation="MDIRQVTETIAMIEEQNFDIRTITMGISLLDCIDPDINRAAEKI
                     YQKITTKAANLVAVGDEIAAELGIPIVNKRVSVTPISLIGAATDATDYVVLAKALDKA
                     AKEIGVDFIGGFSALVQKGYQKGDEILINSIPRALAETDKVCSSVNIGSTKSGINMTA
                     VADMGRIIKETANLSDMGVAKLVVFANAVEDNPFMAGAFHGVGEADVIINVGVSGPGV
                     VKRALEKVRGQSFDVVAETVKKTAFKITRIGQLVGQMASERLGVEFGIVDLSLAPTPA
                     VGDSVARVLEEMGLETVGTHGTTAALALLNDQVKKGGVMACNQVGGLSGAFIPVSEDE
                     GMIAAVQNGSLNLEKLEAMTAICSVGLDMIAIPEDTPAETIAAMIADEAAIGVINMKT
                     TAVRIIPKGKEGDMIEFGGLLGTAPVMRVNGASSVDFISRGGQIPAPIHSFKN"
BASE COUNT        17676 a        11132 c        13000 g        16890 t
ORIGIN      
        1 atactcatct cttgctgccc tagggcttgt tgctgcaggt gtgctagcag cttgctcagg
       61 tggtgctaag aaagaaggac aagcagctag caagaaagaa attattgttg caactaattc
      121 ttcaccaaaa ccattcaact atgaagaaaa tggcgaattg actggttacg agattgaagt
      181 cgttcgcgct atctttaaag actctgacaa gtatgatgtc aagtttgaga agacagagtg
      241 gtcaggtgtc tttgcgggtc ttgacgctga ccgttaccaa atggctgtta acaatatcag
      301 ctacactaaa gaacgtgcgg agaaatacct ttatgcagcc ccaactgtta aagaccctaa
      361 cgttcttgta gtcaagaaag acgattctag catcaaatcg cttgatgatg ttggtggaaa
      421 atcaacagaa gttgttcaag gaacaacatc tgctaaacag ttagaagact acaacaaaca
      481 acactcagat aatccaacta tccttaacta tactaaagca aacttacaac aaatcatggg
      541 ccacttgagc gatggtcagt ttgactacaa gatttttgat aaaatcactg ttgaaacagt
      601 catcaagaac caaggtttgg acaacttgaa agtcatcgat cttccaagcg accaacaacc
      661 gtacgtttac ccacttcttg ctaaaggtca agatgagttg aaaacatttg tagacaaacg
      721 tatccaagaa ctctacaaag acggaactct tgaaaaattg tctaaacaat tcttcggaga
      781 cacttaccta ccggcagaag ctgatattaa ataatttctt gaaatcatcc attctgagta
      841 agatgggtga tttttcagtt cgctactgca atctagtcgg aacagttctc tttggagtgg
      901 tcttgattgc catcttgcgt ttggtctttt aaattaccca taaaaaaaac ttatcacaga
      961 gatagatatc atataatggc gtaaatgctc tttttctgtt aagattataa ggtattctat
     1021 tttggaggaa atgacatgaa aaaaatcgtt aaatactcat ctcttgctgc cctaggactt
     1081 gttgctgcag gtgtgcttgc ggcttgctca ggtggtgcta agaaagaagg agaagcagct
     1141 agcaagaaag aaatcatcgt tgcaaccaat ggatcaccaa ggccatttat ctatgaagaa
     1201 aatggcgaat tgactggtta cgagattgaa gtcgttcgcg ctatctttaa agattctgac
     1261 aaatatgatg tcaagtttga aaagacagaa tggtcaggtg tctttgctgg tcttgacgct
     1321 gaccgttaca atatggctgt caacaatctt agctacacta aagaacgtgc ggagaaatac
     1381 ctctatgccg caccaattgc ccaaaatcct aatgtccttg tcgtgaagaa agatgactct
     1441 agtatcaagt ctctcgatga tatcggtgga aaatcgacgg aagtcgttca agccactaca
     1501 tcagctaagc agttagaagc atacaatgct gaacacacgg acaacccaac tatccttaac
     1561 tatactaagg cagacttcca acaaatcatg gtacgtttga gcgatggaca atttgactac
     1621 aagatttttg ataaaatcgg tgttgaaaca gtgatcaaga accaaggttt ggacaacttg
     1681 aaagttatcg aacttccaag cgaccaacaa ccgtacgttt acccacttct tgctcaaggt
     1741 caagatgagt tgaaatcatt tgtagacaaa cgcattaaag aactctacaa agacggaacg
     1801 cttgaaaaat tgtctaaaca attcttcgga gatacttatc taccagcaga ggctgatatt
     1861 aaataatttc ttgaaatcat ccattctgag taagatgggt gatttctcag tttttaggga
     1921 tatagtttta aactatatat ctgattatct aataacaatt tgtgaaatca aacaaattgt
     1981 gagatactag tacggtatta tttttaagga gaaagaatca tgaaaatcaa aaaatggctt
     2041 ggcctagtag cccttgctac agtcgcaggt ttggctcttg cagcttgcgg aaactcagaa
     2101 aagaaagcag acaatgcaac aactatcaaa atcgcaactg ttaaccgtag cggttctgaa
     2161 gaaaaacgtt gggacaaaat ccaagaattg gttaaaaaag acggaattac cttggaattt
     2221 acagagttca cagactactc acaaccaaac aaagcaactg ctgatggcga agtagatttg
     2281 aacgctttcc aacactataa cttcttgaac aactggaaca aagaaaacgg aaaagacctt
     2341 gtagcgattg cagatactta catctctcca atccgccttt actcaggttt gaatggaagt
     2401 gccaacaagt acactaaagt agaagacatc ccagcaaacg gagaaatcgc tgtaccgaat
     2461 gacgctacaa acgaaagccg tgcgctttac ttgcttcaat cagctggctt gattaaattg
     2521 gatgtttctg gaactgctct tgcaacagtt gccaacatca aagaaaatcc aaagaacttg
     2581 aaaatcactg aattggacgc tagccaaaca gctcgttcat tgtcatcagt tgacgctgcc
     2641 gttgtaaaca ataccttcgt tacagaagca aaattggact acaagaaagc acttttcaaa
     2701 gaacaagctg atgaaaactc aaaacaatgg tacaacatca ttgttgcgaa aaaagattgg
     2761 gaaacatcac ctaaggctga tgctatcaag aaagtaatcg cagcttacca cacagatgac
     2821 gtgaaaaaag ttatcgaaga atcatcagat ggtttggatc aaccagtttg gtaataagaa
     2881 acagggaggt gggagagaaa attccacctc ttgcttttgt atagagtata gattgtaaag
     2941 aagactattc gttcatagaa aggtagagag aatatggttt ttcctagcga acaagaacag
     3001 attgaaaaat ttgaaaagga tcatgtagcc cagcattatt ttgaggtttt gcgtaccttg
     3061 atttctaaga aatcagtctt tgcccagcag gttggactca aggaagtcgc aaattatctg
     3121 ggtgagattt tcaagcgtgt tggagctgaa gtggagattg atgagagcta tacagcgccc
     3181 tttgtcatgg cacatttcaa gagttcgcgt ccagatgcca agaccttgat tttctataac
     3241 cactatgaca ctgtgccagc ggatggggat caggtctgga cagaggatcc ttttacgctt
     3301 tcggtccgca atggcttcat gtatgggcgt ggggttgatg acgacaaggg tcatatcaca
     3361 gctcgcttga gtgctttgag aaaatatatg cagcaccatg atgatttacc tgtcaatatc
     3421 agctttatca tggagggagc ggaggaatcg gcttcaacag acctagataa gtatttggaa
     3481 aagcatgcag acaaactccg tggggcggat ttgttggtct gggaacaagg gaccaaaaat
     3541 gccttggaac agctggaaat ttctggtggc aataagggga ttgtgacctt tgatgccaag
     3601 gtaaaaagcg ctgatgtgga tatccactcg agttatggtg gtgttgtgga atcagctcct
     3661 tggtatctcc tccaagcctt acagtctctt cgtgccgcgg atggccgtat cttggttgaa
     3721 ggcttgtacg aagaagtaca agagcccaat gaacgagaaa tggccttgct agaaacttat
     3781 ggtcaacgaa acccagagga agttagtcgg atttatggat tggagttgcc tctcttacag
     3841 gaggagcgga tggcctttct aaaacgtttc tttttcgagc cagcgcttaa tatcgaagga
     3901 atccagtctg gttatcaagg tcagggtgtt aagactattt tacctgcaga agccagtgcc
     3961 aagctagagg ttcgtctggt tccgggccta gaaccgcatg atgttctgga aaaaattcgg
     4021 aaacagctag acaaaaatgg ctttgataag gtagaattat actatacctt gggagagatg
     4081 agctatcgaa gcgatatgag cgcaccagcc attctcaatg tgatcgagtt ggccaagaaa
     4141 ttctatccac agggcgtttc agtcttgccg acgacagcgg ggacaggacc tatgcatacg
     4201 gtctttgatg ccctagaggt accaatggtt gcattcggtc taggaaatgc caatagccga
     4261 gaccacggtg gagatgaaaa tgtgcgaatc gctgattatt acacccatat cgaattagta
     4321 gaggagctga ttagaagcta tgagtagaga tattatcaag ttagatcaga tcgatgtgac
     4381 ttttcaccaa aagaagagaa ccatcacagc ggttaaggat gtgactattc acatccaaga
     4441 aggggatatc tacggaatcg ttggatattc tggagcaggg aaatcaaccc ttgtacgggt
     4501 gattaacctc ttgcaaaaac catctgcagg gaaaattacc attgacgacg atgtgatttt
     4561 tgatggtaag gtcaccttga cggcagagca gttgcgtcgt aaacgtcaag atatcgggat
     4621 gattttccag cattttaacc tgatgagcca aaagacagca gaggagaatg tagcctttgc
     4681 ccttaaacac tctggactta gcaaggaaga aaagaaggct aaagtagcta agttgttgga
     4741 cttggttggt ttggcagatc gtgctgaaaa ctacccttca caactatctg gagggcaaaa
     4801 acagcgtgtg gcaattgcgc gtgccttggc caatgatcca aaaatcttga tttcagacga
     4861 gtcaacttct gcccttgacc ctaagacaac caagcagatt ttggccttgt tgcaagattt
     4921 gaaccaaaaa ttaggcttga ctgttgtctt gattacgcat gaaatgcaga ttgtcaaaga
     4981 cattgccaac cgtgttgcag ttatgcagga tgggcatttg attgaagagg gtagtgtgct
     5041 tgaaatcttc tcagacccta aacaaccttt gactcaagac tttatctcaa cagctacagg
     5101 tattgacgaa gccatggtca aaatcgagaa gcaagaaatc gtggaacact tgtctgaaaa
     5161 cagtctcttg gtgcaactca agtacgctgg agcttcaaca gacgagccac ttttgaatga
     5221 attgtacaag cattaccaag taatggctaa tattctctat gggaatatcg aaattctcga
     5281 tggtactcct gttggagaat tggtggtggt cttgtcaggt gaaaaagcag cgttggcagg
     5341 tgcccaagaa gccattcgtc aagcaggtgt acaactaaaa gtattgaagg gagtacagta
     5401 agatggaatc attgattcaa acctatttac caaatgtcta taaaatgggt tgggctggtc
     5461 aggcaggctg gggaacggct atctacttaa ctctttatat gacagttctt tccttcatta
     5521 tcggaggctt cttggggcta gtggcaggtc tctttctcgt cttgacagcg ccaggtggtg
     5581 tcttggagaa taaagtcgta ttctggattt tagacaaaat tacctcaatt tttcgtgcgg
     5641 ttccctttat catcctcttg gcaatcttgt caccactttc tcacttgatt gttaaaacaa
     5701 gtatcgggcc aaatgcagcc cttgtcccac tttcttttgc agtctttgcc ttctttgccc
     5761 gtcaggtgca ggttgtcttg gctgaattgg atggcggtgt cattgaggcg gctcaagcga
     5821 gcggagcgac tttctgggac atcgtgggtg tttacctatc agaaggtctt ccagatttga
     5881 tccgtgtgac gactgtgacc ttgatttccc ttgttgggga aacagctatg gccggtgcgg
     5941 ttggagctgg tggtatcggt aacgtagcca tcgcttatgg atttaaccgc tacaatcacg
     6001 atgtgaccat cttggcaacc atcattatca ttttgattat ctttgcaatc caattcttgg
     6061 gagatttctt gactaagaaa ttgagccata aataaaaaag agccgcgtgg ctctttttaa
     6121 ctgatcagat tttctgtgca aattttttac tcaaggcttg tccaatcaag gcacccacta
     6181 gggctccgat gacaatactt gcgataaata gaaggacagt tccagggttt ggagcgacca
     6241 tgatacggtc gatatattct tgggattttc ctcttgccag aagagtagcc atataggctt
     6301 tgggcgcaat ccacataagc aagattggtc ctgttgtact aaaggcgaaa ataatgaaag
     6361 aaaggaagtt ctttgttttg tccttgtatt ttcctaattg agctacttca tctgctagga
     6421 ggccacagat aattccagga aggaaggaac cagcaccgtg tttagttccc aagaaaaaga
     6481 gggcaatgac aaggccgata gtggtaatgg ctccaaagcg cggaactttt gcgactagga
     6541 tcatatagac gctaccgccg acaagggcag taaaggcagg cgcataaaac atgtttccag
     6601 tttggtcaaa gagattgccc aaaaggacac caatccccat gcagaggaag taaagaactg
     6661 caaaaagcag tgtagttaag atagatttct tcatgaattg tctcctgata attttttcac
     6721 aattctcatt gtaccacggt ttggtgggat tgtaaatgga tactagaagt ttttgaaaac
     6781 gcttgaaata tgatataatg gtttcatagt tatttttagg ggaatatcat gggtagattt
     6841 ttagactttg tctttaatcg tttcttttta gggatgattg cgacagcctt cttttggcta
     6901 ttaactttag taggagggat tatccttggt ctagcgccgg ctagtgccac cttgatgagc
     6961 ttatatgcag aacatggtta tagctttcgg gaatacagtt tgaaggaggc ttggtctctt
     7021 tacaagcaaa attttgtctc aagcaacctg attttctata gctttttagg tgtgggtcta
     7081 gttttgacct atggtttgta tctcttggtg caattgcctc atcagaccat tgttcatttg
     7141 attgcgaccc ttttgaatgt cctagtagtt gccctgatct ttttggctta tacagtatct
     7201 ttaaaattac aagtttattt tgccttgtcc tatcgaaata gtctcaaatt atccttgatt
     7261 ggcatcttta tgagtctagc agctgttgcc aaggttctcc ttgggactgt gctacttgta
     7321 gcaattggtt actatatgcc tgccctgcta ttttttgtag gaattgggat gtggcatttc
     7381 tttatcagtg atatgttgga acctgtctat gaaatcatcc atgaaaaatt ggcgacaaaa
     7441 tagaatgaag cagttttggc tacatacgct tctaagaacc tatagttcag tgatgatcat
     7501 tatcattgcg agttttgcaa tcttactctc ttacgctgac tgggattcac gtgaaaagga
     7561 agcccagaga gtagcccagc gtgtaactgc tagaacagtg agtgaaattg aatattacca
     7621 tagagagtca acccagatag ctcaggcttt agttgaaaat caagctcgta ttgagggaat
     7681 ctataaatac tttagcctta gcatgccaga ctatttttac tggcaattag agcggaaagc
     7741 ttcgccttat atatcagtct ctctgtatga aaatgttgat gacctctatg ttcgaaatga
     7801 ttttgtaact ggagtggcca ttgcttttca agattacaag gaagtctatg tttctactaa
     7861 agacaaacgt agtggagaaa aaatcagggc tgaggatttc aaaccagtag gaaatagttt
     7921 tgccattccg gtgtcagatc cagtgtcaga tcaagattta ggagtgattt acatctcctt
     7981 ggatcctgct gttttatacc atgccattga taatactaga ggtcatactc cgatggtagt
     8041 aacagtgacc tcaccttttg atacggagat ttttcatatt ggtgagacag ttgataagga
     8101 gagtgaaaat tggctagttg gcttaacttc tcatggatat caggttcagg tggcagttcc
     8161 taaaaacttt gttttacaag gaacagtgac tagctctgct ttgattgtgg gtttgagcct
     8221 tctctttatt gtcattcttt atctgacttt gaggcagact tttgctaatt accaaaagca
     8281 ggtagtggat ttagtagaat ccattcaagt cattgctcaa ggcgaagagg ggcgtcggat
     8341 tgacatttcc gagaaagatc aggaattact cctaatcgcg gagacgacca atgatatgtt
     8401 ggatcgattg gaaaagaata tccatgatat ttaccagtta gagcttagtc aaaaagatgc
     8461 caatatgcga gccttgcagg cgcaaatcaa tcctcatttt atgtataata cgctggagtt
     8521 cttgcgcatg tatgcagtta tgcagagtca agatgagttg gcagatatca tttatgaatt
     8581 cagtagtctc ttgcgtaaca atatttccga cgaaagagag accctcctca aacaggaatt
     8641 agaattttgc cgtaaataca gctatctctg catggttcgc tatcccaagt ccattgccta
     8701 tggtttcaag atagatccag agttagagaa tatgaagatt cccaagttta ccttgcaacc
     8761 gctggtagaa aactatttcg cgcatggtgt tgaccacagg cggacagata atgtgattag
     8821 catcaaggct cttaaacagg atgggtttgt ggaaattttg gtggtcgata atggtagagg
     8881 aatgtcggct gaaaagttgg caaatatccg agaaaaatta agtcagagat attttgaaca
     8941 ccaagccagc tacagtgatc aaaggcagtc tatcgggatt gtcaatgtac acgagcgttt
     9001 tgtgctctat tttggagacc gctatgccat tactatagag tctgcagagc aagccggtgt
     9061 tcagtatcgt attacaattc aagatgagta gaaagggaga aaatgtataa agtattatta
     9121 gtagatgatg agtacatggt gacagaaggt ctgaagcgtt tgattccctt tgataagtgg
     9181 gatatggagg tcgtcgcaac agccagtcat gccgatgaag ctctagaata tgttcaggaa
     9241 aatcctgtcg atgtcatcat ttccgatgtc aatatgccag acaaaacagg acttgatatg
     9301 attcgggaga tgaaagagat cttaccagat gctgcctata tcctgctctc aggttatcag
     9361 gagtttgatt atgtaaaaag agcaatgaac cttagtgtgg tggactattt ggtcaagcct
     9421 gttgataagg tagagctggg aaatctgctg gagaagattg caggtcagct cggcgagaga
     9481 gggaagaaaa gtcagactct tagtcaagaa ttagacgagg ctggatttgt tagttattta
     9541 ggggataagg agaattggtg gataggtcta tccaaggaaa aacaaggttc cttcaccatt
     9601 ccctactatg tcttgggtca agactggcag attttcattt ctggccaccc cctagatggt
     9661 ttagtcgtta caccttttga agctccttat caagaacatt ttgaacgctg gaagctgaat
     9721 gctgagaaaa ccctctttta cggttctgta aatctgcagc agtctgagag tctctttgcc
     9781 tattacgaac cgatttatag ggttatcatt cagggaaatc tcaatcaaat cgtagaagag
     9841 ttaaatctct tggagaaggt agttcttgaa aatacacctc gtgtttcgat tactaaacag
     9901 ctttttatcc agtttgtcat ggatgttttc catttatttg aacatctcaa agctgatgat
     9961 atgacggaca ttgtcaaaac cattcatgct attcaatcct tcgatgaatt ggtttcttat
    10021 atcaaggaaa ctctgatcag ctttttcggt caataccgta tgaatgaaaa tgtggtcagt
    10081 gtgctggaag tcattggtcg tgattaccaa aaagagcttt ccctcaagga tatcagtaag
    10141 gccctcttta tcaatcctgt ctatctaggg cagttgatta agcgtgaaac cgattcgacc
    10201 tttgcagagt tactaaataa acaacgtatt aaggctgccc agcagctctt gctttcaact
    10261 agtgacagca tcgaagatat ttgttatgct gttggttaca gtaaccttgg atatttctat
    10321 aaagttttcc gaaaattgtg cggaaaatcg ccaaaagcct accgaaaaca ggtagaaact
    10381 atactataag atttgtattc ctttacaaaa ggtgctataa tattatcagg aggtcctatc
    10441 atgaaaaaga aaccaattta tctatgggtt ttgctaatct tgtctgccct tatttcagtt
    10501 ccgtctctgt ttggaattgt gagtcccttg cctagtaaag aagcccttcg tgctgctcag
    10561 aaacaagttg caggggtcaa tgctcagcaa ttagaagacc agcttaatta tacctataga
    10621 gtagcagaag catctcattc tatttttaat gttgctttga ttgtgctatc tactatttta
    10681 gtggtcgtag cgattgtctt ccttgttcgt aaaaatttgc aatatgcaaa ctatacttac
    10741 gttggctatg ttttgttagc tattattggt tcgatttatg gctatgttgg tttacaagac
    10801 gctgtgcagt tggttcaaga tgagagcatg cgtttgacag tgagtattgg ttcaaaagct
    10861 gttagcattt tctatatcgt catcaatgtt ctcttcctag cccttgtctt ctataagatg
    10921 tggcgacaac agaaagcctt ggcagaagaa gaggaaacag aagagcttac ctaagtattg
    10981 acaaaaaaga actatcaaga tacaatcttg gtagttgttt tttgattata gataaaatca
    11041 tggaggtaca tatgaagaca atttctttag tttatattag tttgagtggc aacactgaaa
    11101 gttttgtgac gcgcttgaaa gactatctct tgtcccagta caaagggatt gaggttcaaa
    11161 agattcatat caaggatttg gtcaaggaag gcaaaaattt ctatgaaatg gaccatccct
    11221 atgtcgcttt tttgccgacc tatctcgaag gtgggaatgg cgtggataac ggagatgttg
    11281 agattttgac gacaccggtg ggagatttta ttgcctatgg taacaatgct agtaagtgtt
    11341 ttggtgtggt tgggtcagga aatcgtaact ttaataacca atactgccta acagccaagc
    11401 aatacagtca acgctttggc ttccctgtat tggctgactt tgaaatgcga ggcatgctgg
    11461 aagatatcaa acatgtcgca gctattatcg cagatttgta tgagttggaa aaggagaatt
    11521 aatactcaat gaaaatcaaa gagcaaacta ggaagctagt cgcaggttgc tcaaagcaca
    11581 gctttgaggt tgcagataga gctgacgtgg tttgaagaga ttctcgaata gtataaagtt
    11641 cttataagag gtggttgaag ttgttcaact gttttttgag tataaacagt ctttaagaac
    11701 gtaaaaaagc ataagttcag gtcttcaaaa ccttgttctt atgcttttta tcatagaaat
    11761 atttgctgaa ttttctcttg aaatgaaacc ttatcttcat ctcgcgcttg tcccttaccc
    11821 tgcttttccg attgcgagtg cgtagataaa gaagatggcg aagccaaaga ggaaaatcaa
    11881 tcccgcaatg gcaaggtgtt tgagccaaga aggaagattt ttgttttcac gcgttaccaa
    11941 ggcgtaattc aaaagagcaa agaaaggtgt tgtcaggaaa gagccaatca tggcaaagcg
    12001 gagcatggtt gaaacctgac cagcgaagaa cttgataatg acgataccga tgatagcagt
    12061 gatggtcatc cagatgttca aagatttacg attgtcctct ttttgactga ttagcagtcg
    12121 gagagattcc tgattaacgc gagaatagcc atcgataaca gttataactg ttccaaagat
    12181 acagaggaag gcaataaagg taatcaagta acgggaccat tcgccaagaa cagaggcata
    12241 catgcccacg aattgagaga tgtatttggc tgaagcagct tcaaccgcct gccctgtagg
    12301 atactgaatc agtgctccca gtgccacaaa gaagacggct aggatagctg ttccaatata
    12361 accagtgtta aagtcaaaca gagcgtcttc tgtgttaaag ttgacggtct ttctcttttc
    12421 agctgaccaa agtgaattga tggctgaaat ttcaatagga gccggcatcc atcctaggag
    12481 ggagacgatg aagggcagag ctgccatttg ccaaggtgtc ttctcgacaa aatcagaact
    12541 gtattctgga tgcttgaccg ccgcaatgat aactgcaaga acagtcgcaa tggttaaggc
    12601 agacataatc catttgacca tgccgtctaa aagtttgtag cctccaaaga gtagcatagc
    12661 ccaaatgatt gcaacgagaa tgagggacca ctgagtaatg ctaagtccaa tcattgggaa
    12721 ggcactggcg atgatagctg agcacagaat ggcaacacca gccgtgttga ccatagccga
    12781 aaagacattg aggataaaga aaatccagag atagagtttt cctttttcgg cataaccttc
    12841 aaccaaagtc tttccagtat cagctgtgta ttcagcacca aaacggaaaa atggatattt
    12901 aaagacattg gctaagatga ccaagagaag tagagaccaa ccgtaagaac cgccagcttg
    12961 agttgaggat acaatgtggg aacctccaac agcggcagtt gccattagga ttccaggtcc
    13021 cattgctttt agcttgcttg cccaggttga ttgatttaag gaaatagctt gcgacatgat
    13081 aaatactcct ttttgaattt tcagaataat ctgtatatgt aatctatatt ctacaaaaaa
    13141 ttttgaaaaa atgcaagagt attttgaaaa aagatagaaa atttacaaag aaaatctgaa
    13201 ataactgtta tgatatagag gattgaaact agaatagtac acctctgctt ctaaaacatt
    13261 gttagaaatc gatttgactg tcctgatcga tttatcctgt tcttatttca tttcactata
    13321 gtaaggtgca gcaggataaa gaaaaaccga gaagtttctt tctcggattt gcttattatg
    13381 aggttcctat tttctatgct ccaagagttg attgatgtga tctacttttt tagcctctct
    13441 tttatagaga atagcccaga tgatgagata gacaatctca aactcgataa tgagctggag
    13501 gtagaaaatc cagtggaaag ggaaccaacc agcaagagtt gctagtggga caaagccagc
    13561 cagcataagg aagaaatgag tcagagtggc acgaagcatg ctccagtcac ggctaaataa
    13621 gcggttgcca aagttgaaga gcataccgat ggttgcccag ataagtgtgc agtagagcaa
    13681 gaccagggca ccgtgaacct gatgctgatc catcacttgg ccgatgagag agtagggatt
    13741 tagtggtgcg taggtatttg gtgcataaat gagtgaaaag aggatagaga ggatgaggcc
    13801 gataagaaca ccggtagctg catcgtgaaa tacttgtttt ttcatagttc taatttctcc
    13861 ttgatggttt ttagataacg gcgtgaagag taggtgaagc tttcgttttt caagaaaatt
    13921 tctaccagac cgtttggcgt gagcttgagg tgagagatgg aatcgatatt gatgatttct
    13981 gattgggaaa tttggataaa attgcttggc aagagtttag gaacctgata gagtcgcaaa
    14041 tcaatgctgt aggtctgact cgcggtttct gctagaacct tccgattctc gatatagaag
    14101 cgctgaatct tgccaatctc aactagatag acctgatcat cgcttttccc tttgattttt
    14161 tctctttggt ccagattttc tgcgaactcg atgactttct ggactttatc tgtcggctga
    14221 ggtgcttgga caatcagctt ttcctcctcg taagtctcac taatctgtag ttctactttc
    14281 ataaatttct ctccttgttt ttcatacaaa attaatctca cttcttgtac acctttatta
    14341 atactcttca aaaatctctt caaaccacgt cagcgtcgtc ttgccgtagg atagttgttg
    14401 actttgtcag ttttatctac aacctcaaaa cagtattttg aactgtctgc gcctagcttt
    14461 ctagtttgct ctttgatttt tattgagtat accactattt tactccctct ggcaagggac
    14521 tttgtctatg tggagggatt gggctcctat gtggtggagc ttttctgttc tttctgaaat
    14581 atggtataat agcactaatt aatttctagg aaaatagata cagaaagggg ctgaaagatg
    14641 tctcatatta ttgaattgcc agagatgctg gcaaaccaaa tcgcagctgg agaggtcatt
    14701 gaacgtcctg ccagtgtggt caaagagttg gtagaaaatg ccattgacgc gggctctagt
    14761 cagattatca ttgagattga ggaagctggt ctcaagaagg ttcaaatcac ggataacggt
    14821 catggaattg cccacgatga ggtggagttg gccctgcgtc gccatgcgac cagtaagata
    14881 aaaaatcaag cagatctctt tcggattcgg acgcttggtt ttcgtggtga agccttgcct
    14941 tctattgcgt ctgttagtgt cttgactctg ttaacggcgg tggatggtgc tagtcatgga
    15001 accaagttag tcgcgcgtgg gggtgaagtt gaggaagtca tcccagcgac tagtcctgtg
    15061 ggaaccaagg tttgtgtgga ggatctcttt ttcaacacgc ctgcccgtct caagtatatg
    15121 aagagccagc aagcggagtt gtctcatatc attgatattg tcaaccgtct gggcttggcc
    15181 catcctgaga tttcttttag cttgattagt gatggcaagg aaatgacgcg gacagcaggg
    15241 actggtcaat tgcgccaagc aatcgcaggg atttacggtt tggtcagtgc caagaagatg
    15301 attgaaattg agaactctga cctagatttc gaaatttcag gttttgtgtc cttgcctgag
    15361 ttgactcggg ctaaccgcaa ttatatcagc ctcttcatca atggtcgtta tatcaagaac
    15421 ttcctgctca atcgtgctat tttggatggt ttcggaagca agcttatggt tggacgtttt
    15481 ccactggctg tcattcacat ccatatcgac ccttatctag cggatgtcaa tgtgcatcca
    15541 actaagcaag aggtgcggat ttccaaggaa aaagaactga tgactctggt ttcagaagct
    15601 attgcaaata gtctcaagga acaaaccttg attccagatg ccttggaaaa tcttgccaaa
    15661 tcgaccgtgc gcaatcgtga gaaggtggag caaactattc tcccactcaa agaaaatacg
    15721 ctctactatg agaaaactga gccgtcaaga cctagtcaaa ctgaagtagc tgattatcag
    15781 gtagaattga ctgatgaagg gcaggatttg accctgtttg ccaaggaaac cttggaccga
    15841 ttgaccaagc cagcaaaact gcattttgca gagagaaagc ctgctaacta cgaccagcta
    15901 gaccatccag agttagatct tgctagcatc gataaggctt atgacaaact ggagcgagaa
    15961 gaagcatcca gcttcccaga gttggagttt ttcggacaaa tgcacgggac ttatctcttt
    16021 gcccaagggc gagatggact ttacatcata gatcagcacg ctgctcagga acgggtcaag
    16081 tacgaggagt accgtgaaag cattggcaat gttgaccaga gccagcagca actcctagtg
    16141 ccctatatct ttgaatttcc tgcggatgat gccctgcgtc tcaaggaaag aatgcctctc
    16201 ttagaggaag tgggcgtctt tctagcagag tacggagaaa atcaatttat tctacgtgaa
    16261 catcctattt ggatggcaga agaagagatt gaatcaggca tctatgagat gtgcgacatg
    16321 ctccttttga ccaaggaagt ttctatcaag aaataccgag cagagctggc tatcatgatg
    16381 tcttgcaagc gatctatcaa ggccaatcat cgtattgatg atcattcagc tagacaactc
    16441 ctctatcagc tttctcaatg tgacaatccc tataactgtc ctcacggacg tcctgttttg
    16501 gtgcatttta ccaagtcgga tatggaaaag atgttccgac gtattcagga aaatcacacc
    16561 agtctccgtg agttggggaa atattaaaag tataaaaaag tctgggaaaa attttcaaaa
    16621 tcagaaaaac gcataaaatc aggtgttcaa aaaccttgat tttatgcgtt ttatcatgga
    16681 aatagttact tcattttttc ctaattcttt tcgaaactct ttttaaacga cgtcagtttt
    16741 atcagtaatc tcaaaacagt gttttgagct aattttgcca gttttgtctg taacatcgaa
    16801 gttgtgtttt accactctgc gactggtttc ctagtttgct ctatgatttt cacagagtat
    16861 taaattgcga ttttgccaag tttctttatt cgtctaaaag tagagtctgt tctatgcgtc
    16921 taatgtacga attaggttga ccatttcaat agctccttgt gcacactcag aacccttatt
    16981 tcctgctttg gtaccagctc gttctatggc ttgttcaatt gtatctgtcg ttagcacacc
    17041 aaacataaca ggaatttcgc tatttaaact gatttgggcg attcccttag atacctcgct
    17101 acatacataa tcataatgac ttgtattccc tctaatgaca gctcccaagc agataattgc
    17161 atcatatttt ttactttttg ccatttttga tgcaatcagt ggtatttcaa aagctcctgg
    17221 aacccaggct acctcgatat ctttctcgtt tacattctct cttttgagat tatctagtgc
    17281 tccagataat aattttgaag ttataaattc attaaatctc gctacaacaa tacctatttt
    17341 aatattgttt gctactaaat taccttcata agtgttcatt tatttttcct ccatatttaa
    17401 aatgtgaccc attcgatttt tctttgtttc taaataaaaa ctatcgtaag gattggcttc
    17461 tatttcgatt gatattctac tggaaatggt aattccatat ttttctaact gttcaacctt
    17521 gtcaggatta tttgtcagta aatgaagtga ctgaagtccc agatctttaa gcatttgtgc
    17581 tccaatatga tattctctta aatcaccttc aaagcctaat gcaagattgg catcaagcgt
    17641 atccatgcct tgatcttgta aatgataggc ttttaattta ttgataagtc caattcctcg
    17701 tccctcctgt cgcaagtaaa gtaagacacc cgaaccattc tcaacaatca ttttcatagc
    17761 tttatcgaat tgctgtccac aatcgcaacg taaagagcct aaaacatccc ctgttaaaca
    17821 ttcggagtgg acccgacata atacattggc ttcatcctct atgtttccca taataagagc
    17881 aagatgatgt tccccattta gtttatctat atagctaatt gctttgaaat taccgtatct
    17941 agtaggcata ttgacagttg aaactcgttc taccagctga tcatatactt ttctatattc
    18001 ttgtaattct ttgatggtaa ttagtggaat gttgtgtttc ttcgagaact gaattaaatc
    18061 atctgttctc atcattttgc catcatgatt cattatttca caacataggc cacactcttt
    18121 tagtccagct aattttaata aatcaacagt tgcttctgtg tgtccatttc tttctagaac
    18181 accacctttt tttgcaatta aaggaaacat gtgtcctggc ctgcgaaaat cagagggtgt
    18241 tatatcttca gctacacaca tacgtgcggt cagtcctctt tcctcggcag aaatacctgt
    18301 ggtcgtttct ttataatcaa ttgaaactgt aaaagcagtc ttatgattat ctgtattgtt
    18361 ttcaaccata ggtgaaagca ttaattgatt agctaaactt tcgctcatag gcatacaaat
    18421 taatcctttg gcataagtag ccataaaatt aacattttct gttgtagctg cttgtgcaga
    18481 acaaattaag tctccttcat tttctctatc cttgtcgtct ataacaagaa caagtcgtcc
    18541 cttctgcaat gcttctaatg cttcttgtat ttttcgatat tccattgact gattatcctt
    18601 tctgctaaaa tccattttga tataatagtt ccttagatat ttctgatttt ggagagttat
    18661 ccatcagttt ttgcacatat ttacctaaga tatcattttc aagatttact gtactcccga
    18721 cttgtttact cttaagaatg gtttgttcca aggtatgagg gataacagat actgaaaagt
    18781 ttactttgga gactttagcg acagtcagac taatgccgtc aattgtaata gatccttttt
    18841 caactattaa atctaaaatt tctttttgtg tgttgatttg ataccataca gcattatcat
    18901 ctttttttat tgacgagatt tttcctgtac catcaatgtg tcctgtaacg acgtgacccc
    18961 caagtcgacc gttgacagat aaggctcttt ctagattcac ctcacttcca tgttttaata
    19021 gagtaagagc tgttcgactc catgtttcat tcattacatc aactgtaaag gattgatggt
    19081 tgaaatgagt aactgtaaga cagataccat ttactgctat actatcgcct aaatggatat
    19141 ccgttaatat ttttgaggct ttaattgata gtttacaatt acgagagtct ttctgtattc
    19201 tttcaacttt tccgatttct tcaattattc ctgtgaacat ggataaatca cttcactttc
    19261 tatgagatag tcatttccta tttgagaaaa agcataaggt ttcaatctaa tagcgtcatt
    19321 tggcaaagaa atgccttcac ctccgacagg aaacttggca ctgcctccaa aaatttttgg
    19381 tgcaatatat attttcagct catcaacaat ttgttgttcc aaagcactcc aattcattag
    19441 actgccccct tctagaacta ggctatcaat ctgcatgttt cctagatgtt gcattaaact
    19501 cgataagtct atatgattgc cttttttctt tatggaaagt atttcacagc catgattttg
    19561 atatagcttc attttatttt tgtcttcaga ggaagtggca atgtaagttt taatatcatt
    19621 tgctgttttt acgattttag aggtaagagg agttcgtaaa tgtgtatcgc atatgatacg
    19681 gataggattt ttcccttcct ccaatctaca tgtcagcaaa ggatcgtctt gaataacagt
    19741 attgactccc accataattg cactaacata gtgtcgtaac tgatgcacat gctttcttgc
    19801 ttcttcttca gtaatccatt tggattgatt tgttttagtg gctatttttc catccattga
    19861 cattgcatat ttcataaaaa catagggtac atgctgggta atatactttc taaaactttt
    19921 tattaagtta agacactcat tttctaaaat tccaacagta acttgaagat tattttcctc
    19981 aagtatcttt actccttttc cagatacaat aggattacag tctaggcttc caatgactac
    20041 tcttgtaata ccactatcga ttatagcatc tatacaggga ggtgttttcc cgaagtgaca
    20101 acagggttca agtgttacat aaagcgtcgc tccgacaggg gattctctac agtttttaag
    20161 agcatttctc tcagcatgtg ggccaccaaa aaactcatga taaccttgtc cgatgatgtg
    20221 attatctttt acaataactg cgccgaccat aggattggga ttgacgtaac cagccccttt
    20281 ttgtgccagt tttattgcta atttcatata ttttgaatcg ctcatctcac tacctccaaa
    20341 aaaaatatac cttgaatagg ggactactca aggcatacaa aagaaaactt atgcgattaa
    20401 caaaaatact ctgaaatgac aagtaatcat ttcagagcac gcaaaaagca caaatatact
    20461 tttatcttct ttcatccaga ctatactgtc ggctttggaa tttcaccaaa tcatgccttt
    20521 cggctcgtgg gctataccac cggtagggaa ttgcaccctg ccctgaagat agttattcaa
    20581 ttacagatga ttatagtact taattttgaa tatgtcaaca gataaatacc gattgttttt
    20641 gatatactgt atttgtgata atcgattctc gctcctcgga taaagaaaat atgatatact
    20701 agataaacga aataagagag aaggaacact atgtacgcat atttaaaagg aatcattacc
    20761 aaaattactg ccaaatacat tgttcttgaa accaatggta ttggttatat cctgcatgtg
    20821 gccaatcctt atgcctattc aggtcaggtt aatcaggagg ctcagattta tgtgcatcag
    20881 gttgtgcgtg aggacgccca tttgctttat ggatttcgct cagaggatga gaaaaagctc
    20941 tttcttagtc taatttcggt ctctgggatt ggtcctgtat cagctcttgc tattatcgct
    21001 gctgatgaca atgctggctt ggttcaagcc attgaaacca agaacatcac ctacttgacc
    21061 aagttcccta aaattggcaa gaaaacagcc cagcagatgg tgctggactt ggaaggcaag
    21121 gtagtagttg caggagatga ccttcctgcc aaggtcgcag tgcaagcaag tgctgaaaac
    21181 caagaattgg aagaagctat ggaagccatg ttggctctgg gctacaaggc aacagagctc
    21241 aagaaaatca agaaattctt tgaaggaacg acagatacag ctgagaacta tatcaagtcg
    21301 gcccttaaaa tgttggtcaa ataggagcag agaatgacaa aacgttgttc gtgggtcaag
    21361 atgaccaacc cgctctacat cgcctatcat gatgaggagt ggggccagcc cctccatgat
    21421 gaccaagcat tgtttgagtt gttgtgtatg gaaacctatc aggcaggcct gtcttgggaa
    21481 acggtactca acaaacgcca agctttccga gaagcatttc atagctatca aattcactca
    21541 gtcgcagaga tgactgacac tgaattggaa gccatgctgg agaatccagc tatcattcga
    21601 aatagagcca agatttttgc tacacgcgct aacgcccaag cttttctaca gttacaggca
    21661 gagtacggct cttttgatgc ctatctttgg tcttttgttg aggggaaaat tgtcgttaac
    21721 gatgttcctg attatcgcca agcgccagct aaaacaccct tatctgagaa attagccaaa
    21781 gatctcaaaa aacgaggctt caagttcaca ggcccagtcg ccgtattgtc ttttctacag
    21841 gctgcagggc tagttgatga ccacgagaat gattgtgagt ggaaaggtct taaatgatgt
    21901 ctaacaaaaa taagggaatt ctgatttttg cgattctcta tacagtcctc tttgtgtttg
    21961 atggcgttaa attgctggct tctttaatgc catctgtcat tgcaaattat cttgtttatg
    22021 tagttttagc tttatatggc tccttcttgt tcaaggatag attgatccaa caatggaagg
    22081 agattagaaa gactaaaaga aaattcttct ttggcgtctt aacaggatgg ctctttctca
    22141 ttctgatgac tgttgtcttt gaatttgtat cagagatgtt gaagcagttt gtgagactag
    22201 atggacaagg tctaaatcag tctaatattc aaagtacctt tcaagaacaa ccactactga
    22261 tagctgtttt tgcttgtgtc attggacctc tggtagaaga attatttttc cgtcaggtct
    22321 tattgcatta cttgcaggaa cggttgccag gtttactaag cattattctg gtaggacttg
    22381 tttttgctct gactcatatg cacagtttgg ccctatcaga gtggattggt gcagttggtt
    22441 acttaggtgg aggccttgcc ttttctatta tttatgtgaa agaaaaagag aatatctact
    22501 atcccctact tgttcacatg ttaagcaaca gcctctcctt aatcatttta gctatcagta
    22561 tagtaaaatg aaataagaac aggacaaatc aatcaggaca gtcaaatcga tttctaacaa
    22621 tgttttagaa gtagaggtgt actattctag tttcaataca ctgtaacatg tgatgaaaat
    22681 gccagtaatg ataccgagaa aaaagctgag aaacttttcc cagctttatt tgttatagtc
    22741 aaagcgaatg acttgttcct gtgcatctac atgagcatgg accccaaagg gtacaattgc
    22801 tcttggagtt gcgtggccga cattcagatt atagacaatc gggatattgt tgtcaatgat
    22861 atccaatagt gcctctttat agtcgtcatg gaaagtttca tccataggtt ttccgaccaa
    22921 gagtccattg atgaccgcga atatgccagt gtcctttaaa gttagcaaca tctttttgaa
    22981 gtcttctggc ttaggctttt cttctcttgt ttcgagcaag aggattttcc cttcccagtc
    23041 tgacaagtca gggaaaagtt tgtatttttg gcagagctcc gtgctatctg cgtatcgaga
    23101 gttgtcaaag atatcgtaga gggattcgag gcaaccaccg aggattttcc cctcgaactg
    23161 agcacttcct tgcaacaagt caaaacctgt atttgtatga ctgacacgag gtgttcccag
    23221 ggccgtggga ctaaaatcag ttcgttcctc ataccaaacg tcactagggc ggatttctga
    23281 aattcttccc gtctcaatca attctttaaa gtagtgaagg ctataggcta gcatttcttt
    23341 gtctaattca caaatgtctg ctaaaaagga ttgaccataa aaagtcttga ttcctaattt
    23401 atgcaacatg aggtggttca tggttgtatc cgagaagcca agaaaaattt tttgtttgat
    23461 aaccttttgg agttggtcat tttcaaaaag ataaggtagc aagcgatagg tatcgtctcc
    23521 accgatggca cataggatca tgtcgatgct atcatcagaa aaggcatgaa tcaaatcctc
    23581 tgcacgagct tcaggatggt ccttgataaa gtctaatcct tttaacgaat ggggcaaaaa
    23641 gatgggattg agtcccagat ccttgagacg ttggataccc aagtccactt cgtgtttgac
    23701 aaaatcctct ccgataatgc cactagataa actaacaata ccaatagtag aaaccatatc
    23761 tcatcctcct agaaatagat tgagcctatt ttatcacaaa atataagaga aagctatgtg
    23821 ggatttcaga agaatacttt taatactctt cgaaaatctc ttcaaatcac gtcagtttta
    23881 tttgcaacct caaagctgtg ctttgagcaa cctgcagcta gcttcctagt ttgctctttg
    23941 attttcattg agtataactc aaaaaagtaa gtgaaaaagc aaccgcacct gcagttgctt
    24001 ttatcgtttt cttattcttg atagcgtgtt ggtcctgcac ttgcactacg gatattcaag
    24061 cgtttcttga gatagaagcg aagggctagg agggctgctc caataatagc tagtggcaat
    24121 ggatccagta ctgggttaag gctagctggt aggaagcttg ttgcaaagaa gacaagcaac
    24181 caaaggaaca tagaagctag gataactagt acagatttcc agaaaggtgg acgttgactg
    24241 cgatccatat ctggtccata gtattggtaa acaaagtagt acatcaagta gaaggcaaat
    24301 ccaccaacca gtccaactaa tagaagagta atcaatccat agccgaaagc ttgatctgcc
    24361 gcaaagaagg ttgtgagggc gctgacaagg gcaaagaggc tagtgatgaa aagagctgag
    24421 tccataatca tcagttttgg gtcatcattt tcttttggat gctctttttc gtactgctct
    24481 ttgacagtga agctatgagc ccaatgagtt ggtgcgccgt atagggaacg ggcagttgta
    24541 cctttagatt gctcctcaag gatttgggga atgacttcct caaaaatagt ctggatttca
    24601 gcgtctgttt tcccatcttt gatgaattgt tgggtagcaa tgtggacaaa ctcttggttt
    24661 ttcttagtta atttttgtag atcaatttga gacataggaa ctcctcttag aaccattttt
    24721 tatggatgag atagagagtg agcgagacac tcatagcaaa ggcgataaag acgattaacc
    24781 agaaggcatt tggctctccg tttaggggaa tttcattatc cttaaagttc atcccgtagg
    24841 cagaaaagac catggttggg atggacatga cgatggtcac aagggccaag gttttcatga
    24901 tgttgttctg gttgttagaa atgatagagg caaaggtctc tgtcatagaa tgcaagacgt
    24961 ttccataaat atctgccatc tcgatggcct gttgggtttc aatcagggtg tcttcaagca
    25021 ggtcttcgtc ctcaaggtat ttcttgatat tgctggttga actggtcaat ttcttaatca
    25081 cgcgctcatt tgttttgagg gaggccttga aatagacgat agttttttcc aattccatga
    25141 gctcaatcaa ttcttcatta cgagttgatt gatgcagttg actttcgatt tgttcactct
    25201 tgcggtcgat tgaacgaagg gctgttaggt aaagctctgc attgcgataa agaatttgaa
    25261 agataaaacg tgaacgcatg aaggtataga aattacgcaa tcgacggttg ataaagacat
    25321 caaggacagg tagtggttcc aaacacgtag tgataatggt ttcctcagtg atgataatac
    25381 caagcgggat ggttacgtag taggtgcggt tatttctttc ctccgtgacc ggcacgtcta
    25441 cgataatcag ggtatactcg tcttcaatgg taatacgaga catttcttcc gcatcgagcg
    25501 gtgctcgaag gtcggcaata tcaatatcga aggtgttggc gatttcgagt gattcatttt
    25561 gagtcggatt gacgagattg atccaagtac ccggttcaag cgtatcgatc tctttaaatt
    25621 cagttgttgt agagagaaaa acttgtttca tatcccttat cctttctcat ttttcagatt
    25681 ttttcacact gtactattat actacaaaat cggccttttg ggtaatagaa tctcactttt
    25741 tttggtataa tggtaagcaa taatggacta gaaagaacaa agatgcaaga taaaattgtc
    25801 attcatgggg cgcgtgccca taatttaaaa aatattgatg tggagattcc gcgagacaag
    25861 ttggttgtcg tgactggctt gtcaggttca gggaagtcca gtctggcttt tgatactctc
    25921 tatgcggagg gacaacgtcg ctatgtagag agtttgtccg cctacgctcg tcagttcttg
    25981 ggaaatatgg aaaaaccaga tgtagatgcc attgatggtc tcagcccagc tatttccatc
    26041 gaccagaaaa cgactagtaa aaatcctcgc tcgacggtgg gaacgacgac tgaaatcaat
    26101 gactatctgc gtctcctcta cgcacgtgtg gggacgcctt actgtatcaa cggacatgga
    26161 gctatcaatg cttcttctgt ggagcaaatc gttgataagg ttttggagtt acctgaaggc
    26221 cagcgcttgc agatcttggc tcctgtcatc cgcaagaaaa aaggccaaca taagagtgtt
    26281 atcgagaagg ttcagaaaga cgggtatgtt cgtgttcgtg tggatgggga agtctatgat
    26341 gtgaccgaag tgccagagtt gtctaagagc aagcaacaca atatcgatgt cgtggttgac
    26401 cgtattgtta tcaaggaggg cattcgtagt cgtctctttg attccattga ggctgccctt
    26461 cgtattgcag aaggttatgt cattatcgac accatggacg attcggagtt gctcttctct
    26521 gagcattatg cctgtccagt ttgtggtttt actgtcccag agttagagcc tcgtctcttc
    26581 tctttcaatg ctccttttgg ctcttgtagt gagtgtgacg gcttgggcat caagctggag
    26641 gtggatactg atttggtagt gccagatgcc agcaaaacct tacgtgaggg agcgctagca
    26701 ccttggaatc ctatctcatc caactactat ccaaacatgt tagagcaggc catgaaagtc
    26761 tttggagtgg ctatggataa gccttttgag gacctgtcag aagaagataa gaacttgatt
    26821 ctctatgggt cagatggtaa ggaattccat ttccactatg agaatgaatt tggtggcgtg
    26881 cgcgatatcg acattccgtt tgagggagtt atcaataata tcaaacgtcg ttaccatgaa
    26941 accaatagcg attacactcg cactcagatg cgtctctaca tgaatgagct gacctgcgga
    27001 acctgtcaag gctatcgtct caatgaccag gccttgtctg tccgtgtggg cggccagcaa
    27061 gggccacata ttggagaaat ctcagacctg tctatcgctg accacttgga cttggtgagc
    27121 cagttaactt tgtctgaaaa tgaagccatc attgctcgtc ccattctcaa ggaaatcaag
    27181 gatcgtttga ccttccttaa taatgtgggt cttaactatc taaccctgtc acgttcagca
    27241 ggaacccttt caggtgggga aagtcagcgc attcgtttgg caacccagat tggttccaac
    27301 ctatcaggcg tcctctatat cctagacgag ccgtcaatcg gtcttcacca gagggacaat
    27361 gaccgcctga ttgccagtct gaaaaagatg cgtgacttgg gcaatactct tatcgtggtg
    27421 gaacacgacg aagataccat gcgtgaagct gattatctga ttgacgttgg tcctggtgcc
    27481 ggtgtttttg gtggtgagat tgttgcagca ggtacaccta agcaagtggc tcgcaacagc
    27541 aagtctatca caggtcagta cttgtcaggc aaacgtgtca tcccagtacc agaagagcgc
    27601 cgtgtcggaa atggtcgttt tattgaagtg ataggagcgc gtgagaacaa cttgcaaaat
    27661 gtcacggctc gctttccact aggaaaattt atcgcagtga caggtgtgtc gggttcaggg
    27721 aaatcgactc taatcaacag cattctcaaa aaagccattg cccagaagct caaccgtaat
    27781 tcagacaaac ctggtaagtt taagactatt acagggattg agcatgtcga ccgtttgatt
    27841 gatattgacc agagccctat cggacggacg ccgaggtcta acccagctac ctatacagga
    27901 gtttttgacg atatacgtga cctttttgct cagacaaatg aagccaagat tcgaggctac
    27961 aagaaggggc gcttcagttt caacgtcaag ggaggtcgct gtgaagcttg ctcaggtgac
    28021 gggattatca agattgagat gcacttcttg ccagatgttt atgtggcttg tgaagtttgc
    28081 cacggaaccc gctataacag tgaaacccta gaagttcact acaaggaaaa gaatatttcg
    28141 caggtcttgg atatgacggt caacgatgcg gtagaatttt tccaacacat tccgaaaatt
    28201 caacgcaaac tccagaccat caaggatgta gggctaggct atgtgacctt gggacagcca
    28261 gctaccaccc tttctggggg agaagcccaa cgtatgaagt tagctagcga actccacaaa
    28321 cgctcgacag gaaaatcttt ctacattctg gatgagccga cgacagggct tcatacagag
    28381 gacattgctc gcctgctcaa ggttttagct cgctttgtag acgatggcaa tacagtcctc
    28441 gtcatcgagc acaatctgga tgttatcaag acggcagacc atatcattga cttgggacct
    28501 gagggcggtg tcggtggtgg aaccatcatc gtaacaggaa ctccagaaga agtagctgcc
    28561 aacgaagcca gctatacagg acactatttg aaaggaaagt tacatcatga ataaacgcgt
    28621 acaagcattt ctagctaaaa tgcaagaaaa agaactagat ggtatcatca tcaacaatct
    28681 taaaaacgtc tattatttga ctggtttttg gggctcaaac ggaacagtct ttatcagccg
    28741 tgaccgtcag gtcttagtga cagactctcg ctatatcata gcagctaagc aagaaaccag
    28801 tggttttgag attgtggctg atcgtgatga attggctgtc attgcaggaa ttgttaagga
    28861 catgggcttg actcgtatcg gttttgaaga tgagatttca gtgtcttatt accaccgtat
    28921 gcaggcagct tttgcaggtt tggacttgct tccacaaact cagtttgtgg aaggtcttcg
    28981 tatgattaag gatgaggcag agattgcagc gattcgcaag gcttgttcta tctcagacca
    29041 agctttccgc gatgcgcttg actttattaa accaggaaaa actgaaattg agattgccaa
    29101 cttccttgac ttccgcatgc gtgagttggg agcatctggc ttatcttttg atacgatcct
    29161 agctagcggt atcaattctt ctaaacccca tgcccatcca atgcacaaac cagtggagtt
    29221 gggagaagcc attaccatgg acttcggctg tctctatgac cactatgtca gtgatatgac
    29281 ccggactatc tatctagggc atgttagcga tgagcaggca gagatttaca atacggttct
    29341 aaaagctaac caagccttga ttgaccaagc taaggcaggc ttaggtttcc gtgactttga
    29401 caaaatccct cgtgatatta tcattgaggc aggttatggc gactacttta ctcacggcat
    29461 tggccacggt attggtctgg atatccatga ggaaccctac ttcagccaga cttctacaga
    29521 aactattaag acaggtatgg ccttgaccga tgaaccaggt atctatatcg aaggcaaata
    29581 tggcgttcgt atcgaagatg atatcctgat tacagagaca ggttgtgaat tattgaccct
    29641 agctccaaaa gagttgatag tcatttagtt gtttgtcaag aaaaaagtac gaaaatgacg
    29701 aaaaaagtca aaaaaaatta aaaataggtc gcaagtcgga tgttttttat ggtataatag
    29761 actaaactga tagtaacatg tagcgaaagg ggtaggtaca tgattaaaat ttatacagtc
    29821 tcaagttgta ctagctgtaa aaaagcaaaa acctggctca atgcccacca gttaagttat
    29881 aaagaacaaa accttggtaa agaaggaatt acgagagaag aattactgga tattctaacc
    29941 aaaacagata acggaatagc cagcatcgtt tcgtctaaaa atcgctatgc caaagccctt
    30001 ggagtggata ttgaagattt gagtgtcaat gaagttctca atctgattat ggaaacaccg
    30061 agaattttaa agagcccaat ccttgtagat gaaaaacgcc tgcaagttgg ctacaaggaa
    30121 gacgatattc gtgccttcct accacgctct gtccgtaatg tagaaaatgc agaagcacgt
    30181 ttgcgtgcag ctctataaac atcaaggctg ggagcacttc ccagtcttat tctattttaa
    30241 tttcaaaaag aaagaagaaa gaaatgaaaa aaatagttct tgttagtcta gctttccttt
    30301 ttgtcctggt tggttgcgga cagaaaaaag aaactggacc agctacaaaa acagaaaaag
    30361 atacgcttca gtcggcattg ccagttattg aaaatgctga gaagaataca gttgtaacta
    30421 agactttggt cttgcccaag tcagatgatg gtagccagca gacacaaaca attacttaca
    30481 aagacaagac ttttttgagt ctaactatcc aacaaaaacg tccagtctct gatgagttga
    30541 agacttatat tgaccaacat ggagtggagg aaactcaaaa agctcttctt gaagcggagg
    30601 agaaggataa gtctatcatt gaagctcgta aactggcagg tttcaaactt gaaacaaaac
    30661 tattgagcgc aacggaactt caaacaacga ctagttttga ttttcaagtt ctggatgtca
    30721 agaaggcttc ccagttggaa catctgaaga atattggttt ggaaaatctt ttgaaaaatg
    30781 aaccaagcaa ctatatttca gatagattgg caaatggcgc gacagaacaa tagaaaatcc
    30841 aaataaatag actgcctgaa ttgtagaagg taaggaatta tgctataatg gtactattct
    30901 aaggaaagag ggtgttagaa tgggatttac tgaagaaaca gtacgtttta aattggacga
    30961 ttccaataaa aaagaaatta gcgaaacttt gacagatgtt tatgcttcgt tgaacgataa
    31021 gggttacaac ccaattaacc aaatcgtagg ttacgtattg agtggagacc ctgcctacgt
    31081 tcctcgttat aataatgcac gaaatcaaat ccgtaagtat gagcgtgatg aaatcgttga
    31141 ggaattggtt cgctactacc tcaaagggca aggagtcgat ctataaccta tgagaattat
    31201 gggattggac gtcggttcaa aaacggtagg ggtggcgatt agcgatccgc ttggttttac
    31261 agctcaaggg cttgaaatca tccagataaa tgaagaacaa ggccaatttg gttttgaccg
    31321 cgttaaggaa ttggttgata cttacaaggt ggaacgattt gtagtgggct tgcctaaaaa
    31381 catgaacaat acaagtggac cgcgcgtaga agctagtcaa gcctacggag caaagctaga
    31441 agagtttttt ggtttaccag tagactatca ggatgaacgc ttgacaacag tggctgctga
    31501 gcgcatgttg attgaacaag cagatatcag tcgcaataag cgcaagaaag tcattgataa
    31561 gttagcagct cagctgattt tacaaaatta tttagataga aaattttaat ataaaggaga
    31621 ggctatgtca cacgatcata accacgacca cgaagaacgt gaactaatca cactagtaga
    31681 tgagcaagga aatgaaacct tgtttgaaat ccttttgaca attgatggaa aagaagaatt
    31741 tggtaaaaac tatgttcttc tagtaccagt taacgcagaa gaagacgaag acggacaagt
    31801 tgaaatccaa gcttactcat tcattgaaaa tgaagatgga acagaaggcg aattgcaacc
    31861 aatcccagaa gactcagaag acgaatggaa catgattgaa gaagtcttca acagctttat
    31921 ggaggagtaa aaacgtccgg gggacgtttt tagccctgcg ctagaaatta gaaacgcagg
    31981 tagtctggga gactgtatca gcccaactcc agaaatgggg aagagttgga acgtccaatg
    32041 aacattttta ccccaactct tagaaattga gaagagttgg aacatctagt ggatgttttt
    32101 agcccatatt cacattcata gtagctagtt attagctaac caggtaagag gtcgggacga
    32161 aaatcctggc cttgttttta ttagtaacca tactttaatg cgataattga ttggactttt
    32221 gtcaaggaga tgtgtatgtt tgaagtagaa gaatggctcc atagtcggat tggtttgaat
    32281 tttcgatcag gtttgggccg aatgcagcaa gcggtggatt tgttaggaaa tcctgagcag
    32341 tcttacccta ttatccacgt aacagggact aatgggaaag gatctaccat tgcttttatg
    32401 agggaattat ttatggggca tggcaaaaaa gttgcgacct ttacctcccc tcatatcgtc
    32461 tctatcaatg accgaatctg cattaatggg caacctatag cagacgcaga ctttatccgt
    32521 ttgactgatc aggtcaagga gatggagaaa acgcttctgc aaactcctgc ccagttgtcc
    32581 ttttttgaat tgctgacctt agttgctttt ctttatttta gggagcaaga ggtggatttg
    32641 gttttattag aagtgggaat tggtggctta cttgacacga ccaatgtggt aactggagag
    32701 cttgctgtca tcacctccat tgggcttgac catcaagaaa ccttgggtga tagtctagaa
    32761 gcaattgcag agcagaaagc tggtattttc aaggctggta agaaggcagt gattgcgaaa
    32821 ttgcctccag aagctaggct tgtctgtcag aaaaaagccg aatctttagc tgttaacctt
    32881 tatcaggcag gtcaagattt tttaatgctg aatggtgatt tttcaagctc tttactaaat
    32941 ctttcacagc tgaacatagg cttagaagga gtctatcagc aggagaatgc agccttggcg
    33001 ttgcaaactt ttcttctttt tatgggagaa ggaaaggaag ctgttgatga acaggctgta
    33061 agaaaggcct tggaacagac ccattgggct ggtcgcttgg agcgtattcg cccacagatt
    33121 tacttggatg gtgctcataa cctccctgcc ttgactcgct tggttgagtt tatcaaagaa
    33181 aaagagcagg aaggctatcg acctcaaatc ctttttggag ccttgaaacg gaaggattat
    33241 caagggatgt taggttacct gactgaaaaa ttgcctcagg tggaactcaa ggtgaccggc
    33301 tttgactatc agggggcttt ggacgaaagg gatgtgacag gttacgatat agtttcttct
    33361 taccgagaat ttatcagcga ttttgaagaa agagcagatg ctcaggattt gctgttcgtt
    33421 acagggtctc tctattttat ctcagaagta cggggctacc tgctggaccg tgagcagata
    33481 aattgacccc cttgtttgaa cttgttatac taggggaatt gctggttaga gaaaatttct
    33541 ctaaattggt agcagaaagg aaattcatca tgaaattaaa aagattcaca ctttctcttg
    33601 cttctctagc aagttttagt ctcttagtag cttgttcaca aagagttcaa caggttcaac
    33661 agcctgttgc tcagcagcag gtccaacagc ctgctcaaca gaataccaat actgcaaatg
    33721 caggaggtaa ccaaaatcaa gcggctccag tacaaaacca acctgttgct caaccgaccg
    33781 atattgatgg gacttatact ggtcgggatg acggagaccg tatcacttta gtggtaactg
    33841 gaacgactgg tacatggact gagctcgaat ctgacgggga tcagaaagtc aaacaggtta
    33901 catttgattc agcaaatcaa cgcatgatta ttggcgatga tgtcaaaatt tacactgtaa
    33961 acggtaatca aatcgtcgta gatgatatgg atagagaccc atcggaccaa atcgttttaa
    34021 ctaaataaga ctatgcaagt agaaagggct ggatttttca gcctttttac ttttacctag
    34081 aaaataatca taaatacttg ccttctaccg aatacctgcg ttatactagt atcaattact
    34141 tgtttttagc attcaaacta tcaaggaggg gatatgaaat ataggaaatt tcaattattg
    34201 atgtccaagt atggctttag tctttcgatt atgctacttg aactttgtct tgtttttggt
    34261 ctctttcttt atttaggacg catggctccc attttatgga ttactgtcct cattctactg
    34321 agtatcatca caatcatttc gatagtcaac cgtaatacga ctcctgagaa taaggtaacc
    34381 tggttgttag tagcctttgt gccagtattt ggtcccttgc tctatctgat gtttggtgaa
    34441 aggcgattgt ccaaaaaaga aatcaaacaa ctgaagaagc taggctctat gcatttccaa
    34501 gaagcaaata gccagctact aaaagagaaa ttaaaagaaa gtgacaaggc agcttatgga
    34561 gtcatcaagt ccttattgag tatggatacc aatgctgaca tctatgatca aactgcctct
    34621 acattttttc ctaacggaga agctatgtgg aaaaagatgg tagaagatct taaaaaggct
    34681 gagaaattta ttttcttgga atattacatt atagaagaag gtttgatgtg gaatcgcata
    34741 ctagatatac tagagcaaaa ggtagctcag ggtgtagagg ttaagatgct ctatgatgat
    34801 atcggctgta tggctacttt aacaggagat tatgcacatc gacttcgtca gctgggcatc
    34861 gaggcccata aattcaataa agttattcct cgtttgacag tggcttataa taacagagat
    34921 catagaaaaa tattgattgt tgatggtcag atagcctata ctggtggggt caatctggca
    34981 gatgagtaca ttaaccacgt cgagagattt ggttattgga aggatagtgg aattcgctta
    35041 gacggactag cagtaaaagc cctgacacgc ttatttttga ccacttggta cattaatcga
    35101 ggagaaatta gtgattttga tcaatatcat ttagaaaatc attctatccc gagtgacggt
    35161 ttaaccattc catacggaag tggacccaag ccaatttttc gagcgcaggt agggaaaaaa
    35221 gtttatcaga gtttaatcaa tcaagcaaca gaatcggtct atattacgac accttatttg
    35281 attatagatt atgatttaac agagacaatc aaaaatgcag ctatgagagg ggtcgatgtt
    35341 cgaattatca ccccttgcat accagataag aagttcattc agttagtcac gagaggagct
    35401 tatcccgacc ttctttctgc tggtgttcgg atttatgagt atagtccagg ttttattcat
    35461 agtaagcaga tgttggtaga cgaagatttt gcggtggtgg ggacaatcaa tctcgactac
    35521 cggagcttgg tacaccatta tgaaaatgca gtcttactct ataaaactcc ttctataagg
    35581 gaaatcgccc gagattttcg aaatatattt gcagattctc aggaagtcta tcctcattct
    35641 atcaaaacga gctggtatca aaagcttgta aaagaaatcg cccagctatt cgcccctatc
    35701 ttataagaaa tctagaactt aggatacaaa ccagaccgat ttttcttgtc ttttacgaat
    35761 agaaagaaag aggaggggaa ttcatgctaa ctaaggaaga agtgaatgat atgattgagt
    35821 tcaaacagac acatttacac cgctttcggg aaattgaaac ctttgtgaaa ctatgcaaaa
    35881 aatcactcaa acagccatcg caagctgaca atcctaggac tttttgatat actaagacag
    35941 ataaattgaa caggagaaac gatatgagtg tgtatggtag agtagaagaa gttcataagg
    36001 agaatcgtga acctctagaa taccaaatcg aacaagaatc gcatcatcgt gaatcaagtc
    36061 gtcttccttt ggtgaaaatt ttactatgga gtacgcttgt aacggggata actctaggag
    36121 taccgctatt actcgactta atgagtgcac aagaagtgca ggatttttat gcaggttggg
    36181 cgcttcatca gacagggaag atttacagcg actattatgg aagtcaaggt ttgctttatt
    36241 atttgctgac ttacgtgagt cagggcggat ttttctttgc catctttgag tggttagcct
    36301 tggtagcagg aggatttttc ctttttagat cagcggacac cttgacagag caaggagacc
    36361 aagctggaca tctggtgact attttttaca tgctagttac aggtcttgct tttggtggag
    36421 gctatgcgac tcttttagcg cttcctttct tattcgcagc ctttagttta gttgcggctt
    36481 acctaagcaa tccaagccgt gataagggat ttgtacggat tgggctagct ttggcaggcg
    36541 gatttttctt tgctccctta tcatcgctcc tgtttattgc tgtagtgagt ttaggcttgt
    36601 tggtctttaa ccttgggcat agacgctttg cgcatgggtt ttatcagttt cttgcagtgg
    36661 ctttaggttt ttcacttgtc ttttatccaa ctgcctacta tagtgctgca acaggaagtt
    36721 ttggggatgc gattagtggt attcgttatc ctattgacag tattcgcttt gattttactt
    36781 ctaaaatttt agagaatatg tttttttatg gcttgttgtc ccttggtttg ggatttgtgg
    36841 tttgtatctt tttaggtctc tttcaatcca aaccctttaa actatacgtc atttcggttc
    36901 ctgcaagtct tgtggtaatt ttaggtttga ttttactttt cttttcacaa gagcctctgc
    36961 acgcttctta tttgatggtc gtcttccctg ttttcctact tttattggta accaatatta
    37021 agagtcaaca gagggggcgt agtgctagaa gaagccgaag agaaacgcca gttagcctat
    37081 ggagtcgttt cttcaaagga aatctatatc tgctagtttt tgggtttgtc tatcttttgt
    37141 ctgttccttt tttgatgaag tttgtccttt atccagtacc ttatcaagaa cgtaatcgtc
    37201 ttgctgattt ggtaaaagag gagacaaata cggaagatgc tatctatgca tgggatgata
    37261 ctgcgactct ttatcgtaag agtgagcgct tgtcgccatc ggcgattttg tccccgttgc
    37321 actatacagc aactgaagaa aatcgtaata agttacttaa tgacttgaaa gaaaaacaac
    37381 ctaaggtgat tgtggtaaat gacaaggtgg tagtctggtc tgaagtggaa acactcttaa
    37441 aagaaaatta ccaacaagta aagactgatt actcagagtt taaagtctat aaaattaaat
    37501 aaccaaatca atatcttgtg tatttttaaa aattttagga tttttaacac aagatattga
    37561 tttttctttt tagagtggta taatactttt tagaaagaac attttagaaa agagcatgca
    37621 tatgattgca ctagaagaaa aaattacaat tttgccaact ctcttcgtcg agaaacgaga
    37681 tgggagacgt gttgtatttg atgtggacaa gattgacaag gctctccaca aggcggctga
    37741 caaggttatg gatgtgacac ccctggttga aaaacgcctc aatgatctga ctgagcgaat
    37801 tattacagaa attcatagtc gctttccaca gggaattaag atttacgaaa ttcaaaatat
    37861 cgtagaacat gaactccttg aagccaaaga atatgcgctg gctgaggagt atattactta
    37921 tcggacacag agggattttg agcgctcaaa agcgacggat atcaacttta gtattcataa
    37981 acttctcaac aaagaccaga cagttgtcaa tgaaaatgct aataaagaca gtgatgtctt
    38041 taacactcag cgtgatttga cagcagggat tgttgggaaa tcaatcggac tgcaaatgct
    38101 tcctaagcac gtagccaatg cccaccaaaa gggggatatc cactatcacg atttggacta
    38161 cagtccctat acccctatga ccaactgctg tttgattgat tttaagggta tgttggaaaa
    38221 tggttttaag attggaaatg cagaggtaga gagtcccaag tctatccaga ctgcgacagc
    38281 acagatttct caaatcattg ccaacgttgc ttctagccag tacggtggct gttcagctga
    38341 ccgtatcgat gaaattttgg cgccttatgc agagaagaat tatcaaaaac atctcaaaga
    38401 tgcagaagag tgggtattgc ctgaaaaacg ggaagattac gcttggaaga aaacgcaaaa
    38461 ggacatctac gatgccatgc aatctcttga gtatgaaatc aatactctct tcacttcaaa
    38521 tggacaaaca ccttttactt cgttaggttt tggtctggga accagtcgtt ttgaacgaga
    38581 aattcaaaaa gctattttaa acattcgcat caagggtctt ggttcagaac accgtacggc
    38641 tatctttcct aaacttattt tcactctgaa aagaggcctt aatttagagg aaggaactcc
    38701 caactacgac atcaagcagt tggctcttga gtgtgcaacc aaacggatgt atccagatgt
    38761 tttatcttac gataagattg ttgacttgac aggatccttc aaggtgccta tgggttgccg
    38821 ttctttcctc caaggatgga aggatgaaaa tggtgtagaa gtcaattcag gtcgcatgaa
    38881 tctgggtgtt gtgacggtta atctgcctcg tattgctctt gagtctgaag gtgatatgaa
    38941 taagttctgg gaaatcttca acgagcgaat gaatatcgca gaagatgctc ttgtttaccg
    39001 tgtcgaacgc actaaagagg cgacaccagc aaatgctcct attctttatc agtacggtgc
    39061 ttttggccat cgtctaggta aagaagaaag tgttgaccag ctctttaaga atcgtcgtgc
    39121 taccgtttcg ctgggctata tcggcttgta tgaagtagcg acagttttct ttggtaacag
    39181 ctgggaaagt aatccagatg ctaaggaatt cacgctagac atcattcacg atatgaaacg
    39241 ccgtgtagaa gagtggtcag accaatatgg ctaccatttc tctatctact caacaccatc
    39301 cgaaagtctg acagaccgtt tctgccgact agatatagac aagtttggct ctattcctga
    39361 tatcacagac aaggaatact acaccaactc tttccactac gatgttcgta aaaatccaac
    39421 accgtttgaa aaattggact ttgagaaagt ctatccagaa gcaggtgcgt caggtggttt
    39481 catccattat tgtgagtatc cagtccttca gcaaaatcca aaggccttgg aagctgtctg
    39541 ggattatgct tatgaccgtg taggctatct aggcaccaat actccgattg accgttgcta
    39601 caagtgtgac tttgaagggg attttgaacc aactgagaga gggtttgctt gtccaaactg
    39661 tggcaatagc gaccctaaaa cagtagatgt ggtgaagcga acttgtggct acctaggtaa
    39721 tcctcaagca agaccgatgg tcaacgggcg tcacaaggaa atcgctgcgc gtgtcaaaca
    39781 tatgaatggt tcaacgatta aaatagctgg gcatcaagta acaaattaga aagaaatgaa
    39841 atgggaaaat atcaactaga cgataagggg cgcgcacaag tgacccgtta tcacgagaaa
    39901 cactctaaag gtggagctgg taagaaagaa cgcttgctta gcttcagaga acaattttta
    39961 aacaagaaca agaaaaaata aaagtgagag ccagctctcg cttttctcat agtgggaggt
    40021 aaggatggaa ttacgcagac caagattagc ggataagaaa gctgttttag atatgatgac
    40081 agagtttgaa aaatttcagt cgcctcacga cggcggtttc tgggatacag agaactttgt
    40141 gtatgaagac tggttagaaa gcaatcagga acaggaaatg ggaattaatc tgcctgaagg
    40201 atgggttcct gcaattcagt tagtggcttt ttctgagaaa ggtcaagcag ttggatttct
    40261 taatctccgg ttgcgcctca gtaactttct actagaagaa ggtgggcaca ttggctactc
    40321 catccgtcca tctgaaagag gcaagggtta tgcaaaagag actctccgtc agggcttgca
    40381 agttgctaag gaaaagaaca tcaagaaagc tctggtgacc tgtagcgtga ataatcctgc
    40441 tagcagagca gtcattctag caaatggtgg aatatttgag gatgctcgca atggagtcga
    40501 gcgttattgg atagaggtag cgaatgaata atccaaaacc acaagaatgg aaaagcgagg
    40561 aacttagtca aggtcgtatc attgactaca aggcctttaa ctttgttgat ggcgaaggcg
    40621 tgcgtaactc tctctatgta gcaggctgta tgtttcactg cgagggatgt tataatgttg
    40681 cgacttggtc ttttaatgct ggcattccct atacagcaga attagaagag cagatcatgg
    40741 cagaccttgc ccaaccctat gttcaaggct tgactttgct gggaggggag ccttttctca
    40801 atactggcat tctcttgcct ctagttaaac gcatccgaaa ggaattgcca gacaaggaca
    40861 tttggtcctg gacgggctac acttgggaag aaatgatgtt ggaaactcca gataaactgg
    40921 aactcttgtc actgattgac attcttgtcg atggaagata tgatcgaact aagagaaatc
    40981 ttatgctcca gtttcgaggt tcgtccaacc aacgaattat cgatgtgcaa aaatcgctca
    41041 aaagtgggca agtagtgatt tgggacaagc tcaatgacgg aaaagaaagc tatgaacagg
    41101 tgaagagaga atgaagaaaa aagacttagt agaccaacta gtctcagaga tcgagacggg
    41161 gaaagtcagg acactgggaa tatacggtca tggagcttca ggtaaatcaa cctttgcaca
    41221 ggaattgtac caagctttag attctactac agtaaatttg ctagaaacag atccctatat
    41281 cacctccgga cgctatttgg tagtacccaa ggacgcgccg aatcaaaagg tgacagccag
    41341 tctgccagtg gcgcatgaac tggagagttt gcagagagat atccttgcct tgcaggcggg
    41401 tatggatgtc ttgacaattg aagaaccttg gaaggctagt gaggtcttgt ctggagccaa
    41461 accaattttg attgtcgaag ggatgtctgt tggctttcta cccaaggaac tctttgaaaa
    41521 aaccatctgt ttctacacgg atgaggagac cgaattaaag cgacgccttg ctagagatac
    41581 gactgtgaga aatcgcgatg catcctttat attggctagc catcagatga gacgggagca
    41641 gtatctgcga tactataaag aaactgagtc taaggcggat atcttagtgg accaatcaga
    41701 agataaattt gatgtcaaga ggacttaact tatgtataga agaaatatag aagaaaatcc
    41761 ctaattttag aaagaaaaag taggaaaaca gtttcatccc aaaaaaacga aaaaacagca
    41821 acaaatccct tgcaatcgca ggggctttgt gttattctat tatggtgctg taaattacag
    41881 ccttagtttt gatgcaagag gttgcgacac gctcggttgc attgccacgc aacaccgcgt
    41941 cggttttctt gtggagctag cctattatct taaatagacg aaaaggagaa aaagatggca
    42001 aacaaaaaaa tccgtatccg tttgaaagct tacgaacacc gtacgcttga cacagcggct
    42061 gcaaaaatcg tagaatcagc tactcgtaca ggtgcacaag ttgcgggtcc aatcccactt
    42121 ccaactgaac gtagcctcta cacaatcatt cgtgcgactc acaaatacaa agactctcgc
    42181 gaacaatttg aaatgcgtac acacaaacgt ttgatcgata tcgttaaccc aactcaaaaa
    42241 acagttgatg ccttgatgaa attggatctt ccaagtggtg taaacgtaga aatcaaactt
    42301 taatctaaaa tataaaagag cagaggctgg tgtttcaatc taattgaaca cgggctaaac
    42361 tcggtgtgaa aaagataaac ttcctcgcgt ctgctagaca ctgtgtcagt ttcctatttt
    42421 cacgttgagt ttgacgccct ttgtatctta gacttgagca taaaaaacgc tcgttaaaaa
    42481 ctttttgaat aaaaatatag aaaaggaact attttctcat gacaaaagga atcttaggga
    42541 aaaaagtggg aatgactcaa atcttcactg aagctggcga attgatccct gtaacagtta
    42601 ttgaagcaac tccaaacgtt gttcttcaag ttaaaactgt tgaaacagac ggatacaacg
    42661 ctatccaagt tggtttcgat gacaaacgcg aagtattgag caacaaacct gctaaaggac
    42721 atgtagcgaa agctaacacg gctcctaagc gcttcattcg tgaattcaaa aacgttgaag
    42781 gcttggaagt tggtgctgaa attacagttg aaacattcgc agctggagac gttgttgacg
    42841 taacgggtac ttctaaaggt aaaggtttcc aaggtgttat caaacgccac ggacaatcac
    42901 gtggaccaat ggctcacggt tctcgttacc accgtcgtcc aggttctatg gggcctgttg
    42961 cacctaaccg cgtattcaaa ggtaaaaacc ttgcaggacg tatgggtggc gaccgcgtaa
    43021 caattcaaaa ccttgaagtt gtacaagttg ttccagaaaa gaacgttatc cttatcaaag
    43081 gtaacgtacc aggtgctaag aaatctctta tcactatcaa atcagcagtt aaagctggta
    43141 aataataaag aaaggggaaa tcagtcacaa tggcaaacgt aacattattt gaccaaactg
    43201 gtaaagaagc tggccaagtt gttcttagcg atgcagtatt tggtatcgaa ccaaatgaat
    43261 cagttgtgtt tgatgtaatc atcagccaac gcgcaagcct tcgtcaagga acacacgctg
    43321 ttaaaaaccg ctctgcagta tcaggtggtg gacgcaaacc atggcgtcaa aaaggaactg
    43381 gacgtgctcg tcaaggttct atccgctcac cacaatggcg tggtggtggt gttgtcttcg
    43441 gaccaactcc acgttcatac ggctacaaac ttccacaaaa agttcgtcgc ctagctctta
    43501 aatcagttta ctctgaaaaa gttgctgaaa acaaattcgt agctgtagac gctctttcat
    43561 ttacagctcc aaaaactgct gaatttgcaa aagttcttgc agcattgagc atcgattcta
    43621 aagttcttgt tatccttgaa gaaggaaatg aattcgcagc tctttcagct cgtaaccttc
    43681 caaacgtgaa agttgcaact gctacaactg caagtgttct tgacatcgca aatagcgaca
    43741 aacttcttgt cacacaagca gctatctcta aaatcgagga ggttcttgca taatgaattt
    43801 gtatgatgtt atcaaaaaac ctgtcatcac tgaaagctca atggctcaac ttgaagcagg
    43861 gaaatatgta tttgaagttg acactcgtgc acacaaactt ttgatcaagc aagctgttga
    43921 agctgctttc gaaggtgtta aagttgccaa tgttaacaca atcaacgtaa aaccaaaagc
    43981 taaacgtgtt ggacgttaca ctggttttac taacaaaact aaaaaagcta tcatcacact
    44041 tacagctgat tctaaagcaa tcgagttgtt tgctgctgaa gctgaataat ctaaggagga
    44101 aatatcgtgg gaattcgtgt ttataaacca acaacaaacg gtcgccgtaa tatgacttct
    44161 ttggatttcg ctgaaatcac aacaagcact cctgaaaaat cattgcttgt tgcattgaag
    44221 agcaaggctg gtcgtaacaa caacggtcgt atcacagttc gtcaccaagg tggtggacac
    44281 aaacgtttct accgtttggt tgacttcaaa cgtaataaag acaacgttga agcagttgtt
    44341 aaaacaatcg agtacgatcc aaaccgttct gcaaacatcg ctcttgtaca ctacactgat
    44401 ggtgtgaaag catacatcat cgctccaaaa ggtcttgaag taggtcaacg tatcgtttca
    44461 ggtccagaag cagatatcaa agtcggaaac gctcttccac ttgctaacat cccagttggt
    44521 actttgattc acaacatcga gttgaaacca ggtcgtggtg gtgaattggt acgtgctgct
    44581 ggtgcatctg ctcaagtatt gggttctgaa ggtaaatacg ttcttgttcg tcttcaatca
    44641 ggtgaagttc gtatgattct tggaacttgc cgtgctacag ttggtgttgt cggaaacgaa
    44701 caacatggac ttgtaaacct tggtaaagca ggacgtagcc gttggaaagg tatccgccca
    44761 acagttcgtg gttctgtaat gaaccctaac gatcacccac acggtggtgg tgaaggtaag
    44821 gcaccagttg gtcgtaaagc accatctact ccatggggca aacctgctct tggtcttaaa
    44881 actcgtaaca agaaagcgaa atctgacaaa cttatcgttc gtcgtcgcaa cgagaaataa
    44941 tattaaacta gtcgcttaag caactagtaa atccgccagc tcggtagcgc tccataggag
    45001 tgcaagccgc tgtggtacaa catttaaagg agaaaatata aaaatgggac gcagtcttaa
    45061 aaaaggacct ttcgtcgatg agcatttgat gaaaaaagtt gaagctcaag ctaacgacga
    45121 aaagaaaaaa gttattaaaa cttggtcacg tcgatcaacg atcttcccaa gtttcattgg
    45181 ttacactatt gcagtttatg acggacgtaa acacgtacct gtttacatcc aagaagacat
    45241 ggtaggccac aaacttggtg aatttgcacc aactcgtact tacaaaggtc acgctgcaga
    45301 cgacaagaaa acacgtagaa aataaggaga acataaatgg cagaaattac ttcagctaaa
    45361 gcaatggctc gtacagtacg tgtttcacct cgtaaatcac gtcttgttct tgataacatc
    45421 cgtggtaaaa gcgtagccga tgcaatcgca atcttgacat tcactccaaa caaagctgct
    45481 gaaatcatct tgaaagtttt gaactcagct gtagctaacg ctgaaaacaa ctttggtttg
    45541 gataaagcta acttggtagt atctgaagca ttcgcaaacg aaggaccaac tatgaaacgt
    45601 ttccgtccac gtgcgaaagg ttcagcttca ccaatcaaca aacgtacagc tcacatcact
    45661 gtagctgttg cagaaaaata aggaggtaaa atcgtgggtc aaaaagtaca tccaattggt
    45721 atgcgtgtcg gcatcatccg tgattgggat gccaaatggt atgctgaaaa agaatacgcg
    45781 gattaccttc atgaagatct tgcaatccgt aaattcgttc aaaaagaact tgctgacgca
    45841 gcagtttcaa ctattgaaat cgaacgcgca gtaaacaaag ttaacgtttc acttcacact
    45901 gctaaaccag gtatggttat cggtaaaggt ggtgctaacg ttgatgcact ccgtgcaaaa
    45961 cttaacaaat tgactggaaa acaagtacac atcaacatca tcgaaatcaa acaacctgat
    46021 ttggatgctc accttgtagg tgaaggaatt gctcgtcaat tggagcaacg tgttgctttc
    46081 cgtcgtgcac aaaaacaagc aatccaacgt gcaatgcgtg ctggagctaa aggaatcaaa
    46141 actcaagtat caggtcgttt gaacggtgca gatatcgccc gtgctgaagg atactctgaa
    46201 ggaactgttc cgcttcacac acttcgtgca gatatcgatt acgcttggga agaagcagat
    46261 actacatacg gtaaacttgg tgttaaagta tggatctacc gtggtgaagt tcttccagct
    46321 cgtaaaaaca ctaaaggagg taaataacca atgttagtac ctaaacgtgt taaacaccgt
    46381 cgtgaattcc gtggaaaaat gcgcggtgaa gcaaaaggtg gaaaagaagt agcattcggt
    46441 gaatacggtc ttcaagctac aactagccac tggatcacta accgccaaat cgaagctgct
    46501 cgtatcgcca tgactcgtta catgaaacgt ggtggtaaag tttggattaa aatcttccca
    46561 cacaaatcat acactgctaa agctatcggt gtgcgtatgg gatctggtaa aggggcacct
    46621 gaaggttggg tagcaccagt taaacgtggt aaagtgatgt tcgaaatcgc tggtgtatct
    46681 gaagagattg cacgtgaagc gcttcgactt gctagccaca aattgccagt taaatgtaaa
    46741 ttcgtaaaac gtgaagcaga ataaggagaa ggcatgaaac ttaatgaagt aaaagaattt
    46801 gttaaagaac ttcgtggtct ttctcaagaa gaactcgcga agcgcgaaaa cgaattgaaa
    46861 aaagaattgt ttgaacttcg tttccaagct gctactggtc aattggaaca aacagctcgc
    46921 ttgaaagaag ttaaaaaaca aatcgctcgt atcaaaacag ttcaatctga agcgaaataa
    46981 tagactaggg aaggagaaat ttcaatggaa cgcaataatc gtaaagttct tgttggacgt
    47041 gttgtatctg acaaaatgga caagacaatc acagttgtag ttgaaacaaa acgtaaccac
    47101 ccagtctatg gtaaacgtat taactactct aaaaaataca aagctcatga tgaaaacaat
    47161 gttgccaaag aaggcgatat cgtacgtatc atggaaactc gcccgctttc agctacaaaa
    47221 cgtttccgtc ttgtagaagt tgttgaagaa gcggtcatca tctaatcaaa cctgaaagga
    47281 gaaaactgaa atgattcaaa cagaaactcg tttgaaagtc gcagacaaca gcggtgctcg
    47341 cgaaatcttg actatcaaag ttcttggtgg ttcaggacgt aaatttgcaa acatcggtga
    47401 tgttatcgtg gcatctgtaa aacaagctac tcctggtggt gcggttaaaa aaggtgacgt
    47461 tgttaaagca gttatcgttc gtactaaatc aggtgctcgt cgtgctgatg gttcatacat
    47521 caaatttgac gaaaacgcag cagttatcat ccgtgaagac aaaactcctc gcggaacacg
    47581 tatctttggc ccagttgcac gtgaattgcg tgaaggtggc ttcatgaaga tcgtgtcact
    47641 tgctccagaa gtactttaat ttttaggaac aaactagtcc cctagcttca agctagggtg
    47701 cccttatggg cgtaagaaaa atcaaggaga aacctaatgt ttgtaaaaaa aggcgacaaa
    47761 gttcgcgtaa tcgctggtaa agataaggga acagaagctg ttgtccttac tgcccttcca
    47821 aaagtaaaca aagttatcgt tgaaggtgtt aacattgtta agaaacacca acgtccaact
    47881 aacgagcttc ctcaaggtgg tatcatcgag aaagaagcag ctatccacgt atcaaatgtt
    47941 caagttttgg acaaaaatgg tgtagctggt cgtgttggtt acaaatttgt agacggtaaa
    48001 aaagttcgct acaacaaaaa atcaggcgaa gtgcttgatt aatcacgaag gaaaggagaa
    48061 gtataatggc aaatcgttta aaagaaaaat atcttaatga agtagttcct gctttgacag
    48121 aacaattcaa ctactcatca gtgatggctg tgcctaaagt agataagatt gttttgaaca
    48181 tgggtgttgg tgaagctgta tcaaacgcta aaagccttga aaaagctgct gaagaattgg
    48241 cacttatctc aggtcaaaaa ccacttatca ctaaagctaa aaaatcaatc gccggcttcc
    48301 gtcttcgtga aggtgttgcg atcggtgcaa aagttaccct tcgtggtgaa cgtatgtacg
    48361 aattcttgga taaattggta tcagtttcac ttccacgtgt acgtgacttc cacggtgtcc
    48421 caacaaaatc atttgatgga cgcgggaact atacacttgg tgtgaaagaa caattaatct
    48481 tcccagaaat caacttcgat gacgttgaca aaactcgtgg tcttgacatc gttatcgtaa
    48541 caactgctaa cactgacgaa gagtcacgtg cattgcttac aggccttgga atgccttttg
    48601 caaaataata taggaggtaa atctaatggc taaaaaatca atggtagcta gagaggctaa
    48661 acgccaaaaa attgttgacc gttatgctga aaaacgtgct gcattaaagg cggcagggga
    48721 ctacgaaggt ttatctaaat tacctcgcaa cgcctcaccg actcgtttac ataatcgttg
    48781 tagagttacg gggcgcccac attcagttta ccgcaaattt ggtctgagtc gtatcgcttt
    48841 tcgcgaactt gcgcataaag gtcaaattcc tggtgtaaca aaagcatctt ggtaatttaa
    48901 gatatcaagg gtgtcaaaac tccaagtaaa aataggaaac ttgacgaaga aactaaagtt
    48961 tctaggaaag tttatctttt tcacacagag tttagcccgg gttcaattgg gcttgccaat
    49021 ttgaacccga gctacagctt tggcaaaaaa gaccaatttg ctttggagca ttgcttctgc
    49081 attaaattgt ctatttttgc tcgtgctgtt acgctctttg tatcatgtat taactagcaa
    49141 gtgcaacttg caaactacta gtaagaggag aaaaacaaaa tggttatgac tgacccaatc
    49201 gcagacttcc taactcgtat tcgtaatgct aaccaagcta aacacgaagt acttgaagta
    49261 cctgcatcaa acatcaaaaa agggattgct gaaatcctta aacgcgaagg ttttgtaaaa
    49321 aacgttgaaa tcattgaaga tgacaaacaa ggcgtcatcc gtgtatttct taaatacgga
    49381 ccaaatggtg agaaagttat cactaacttg aaacgtgttt ctaaaccagg acttcgtgtc
    49441 tacaaaaaac gtgaagacct tccaaaagtt cttaacggac ttggaattgc catcctttca
    49501 acttctgaag gtttgcttac tgataaagaa gcacgccaaa agaatgttgg tggtgaggtt
    49561 atcgcttacg tttggtaaaa tcaagataca aagctcgtaa agaacaaagc aaaattagga
    49621 agttggagaa gtttgtttac aaacaagcca acttatctat tttgcacagt tcttagagcg
    49681 tgttcagttc agctcttgaa ctaaataagt atctgaaccc cgtgaaaact ggccgttctg
    49741 gcctgacaat ttaacaggag aaaataaaca tgtcacgtat tggtaataaa gttatcgtgt
    49801 tgcctgctgg tgttgaactc gctaacaatg acaacgttgt aactgtaaaa ggacctaaag
    49861 gagaacttac tcgtgagttc tcaaaagata ttgaaatccg tgtggaaggt actgaaataa
    49921 ctcttcaccg tccaaacgat tcaaaagaaa tgaaaactat ccacggaact actcgtgccc
    49981 ttttgaacaa catggttgtt ggtgtatcag aaggattcaa gaaagaactt gaaatgcgtg
    50041 gggttggtta ccgtgcacag cttcaaggat ctaaacttgt tttggctgtt ggtaaatctc
    50101 atccagacga agttgaagct ccagaaggaa ttacttttga acttccaaac ccaacaacaa
    50161 tcgttgttag cggaatttca aaagaagtag ttggtcaaac agctgcttac gtacgtagcc
    50221 ttcgttcacc agaaccatat aaaggtaaag gtatccgtta cgttggtgaa ttcgttcgcc
    50281 gtaaagaagg taaaacaggt aaataatgtt gagtggttga tcatcaacca ccaacctatt
    50341 ttccaacttt gtgcatagca cacgatttaa aactaaagag gtgaaaactg tgatttcaaa
    50401 accagataaa aacaaactcc gccaaaaacg ccaccgtcgc gttcgcggaa aactctctgg
    50461 aactgctgat cgcccacgtt tgaacgtatt ccgttctaat acaggcatct acgctcaagt
    50521 gattgatgac gtagcgggtg taacgctcgc aagtgcttca actcttgata aagaagtttc
    50581 aaaaggaact aaaactgaac aagccgttgc tgtcggtaaa ctcgttgcag aacgtgcaaa
    50641 cgctaaaggt atttcagaag tggtgttcga ccgcggtgga tatctatatc acggacgtgt
    50701 gaaagctttg gctgatgcag ctcgtgaaaa cggattgaaa ttctaatagg aggacactag
    50761 aaaatggcat ttaaagacaa tgcagttgaa ttagaagaac gcgtagttgc tgtcaaccgt
    50821 gttacaaaag ttgttaaagg tggacgtcgt cttcgtttcg cagctcttgt tgttgttggt
    50881 gaccacaatg gtcgcgtagg atttggtact ggtaaagctc aagaagttcc agaagcaatc
    50941 cgtaaagcag tagatgatgc taagaaaaac ttgatcgaag ttcctatggt tggaacaaca
    51001 atcccacacg aagttctttc agaattcggt ggagctaaag tattgttgaa acctgctgta
    51061 gaaggttctg gagttgccgc tggtggtgca gttcgtgccg ttgtggaatt ggcaggtgtg
    51121 gcagatatta catctaaatc acttggttct aacactccaa tcaacattgt tcgtgcaact
    51181 gttgaaggtt tgaaacaatt gaaacgcgct gaagaaattg ctgcccttcg tggtatttca
    51241 gtttctgatt tggcataaga aaggggataa aatggctcaa attaaaatta ctttgactaa
    51301 gtctccaatc ggacgcattc catcacaacg taaaactgtt gtagcacttg gacttggcaa
    51361 attgaacagc tctgttatta aagaagataa cgctgctatc cgtggtatga ttacagcagt
    51421 atctcactta gtaacagttg aagaagtaaa ctaatgaagt tttaggggat gtgcactgta
    51481 ccatccccta aaactagata tagtcatcta tgatgacatc gtataggcga gttgatgggg
    51541 gagacaacct tttctccctt atcggcgcta gcattttaca aaagaggaga aaataaaaat
    51601 gaaacttcat gaattgaaac ctgcagaagg ttctcgtaaa gtacgtaacc gcgttggtcg
    51661 tggtacttca tcaggtaacg gtaaaacatc tggtcgtggt caaaaaggtc aaaaagctcg
    51721 tagcggtggc ggagttcgcc ttggttttga aggtggacaa actccattgt tccgtcgtct
    51781 tccaaaacgt ggattcacta acatcaacgc taaagaatac gcaattgtga accttgacca
    51841 attgaacgtc tttgaagatg gtgctgaagt aactccagtt gttcttatcg aagcaggaat
    51901 tgttaaagct gaaaagtcag gtattaaaat tcttggtaac ggtgagttga ctaagaaatt
    51961 gactgtgaaa gcagctaaat tctctaaatc agctgaagaa gctatcactg ctaaaggtgg
    52021 ttcagtagaa gtcatctaag agaggtgacc tatgtttttt aaattattaa gagaagctct
    52081 taaagtcaag caggttcgat caaaaatttt atttacaatt tttatcgttt tggtctttcg
    52141 tatcggaact agcattacag ttcctggtgt gaatgccaat agcttgaatg ctttaagtgg
    52201 attatccttc ttaaacatgt tgagcttggt gtcggggaat gccctaaaaa acttttcgat
    52261 ttttgcccta ggagttagtc cctatatcac cgcttctatt gttgtccaac tcttgcaaat
    52321 ggatatttta cccaagtttg tagagtgggg taaacaaggg gaagtaggtc gaagaaaatt
    52381 gaatcaagct actcgttata ttgctctagt tctcgctttt gtgcaatcta tcgggattac
    52441 agctggtttt aataccttgg ctggagctca attgattaaa actgctttaa ctccacaagt
    52501 ttttctgacg attggtatca tcttaacagc tggtagtatg attgtcactt ggttgggtga
    52561 gcaaattaca gataagggat acggaaacgg tgtttccatg attatctttg ccgggattgt
    52621 ttcctcaatt ccagagatga ttcagggcat ctatgtggac tactttgtga acgtcccaag
    52681 tagccgtatc acttcatcta tcattttcgt aatcattttg attattactg tattgttgat
    52741 tatttacttt acaacttatg ttcaacaagc agaatacaaa attccaatcc aatatactaa
    52801 ggttgcacaa ggtgctccat ctagctctta ccttccgtta aaggtaaacc ctgctggagt
    52861 tatccctgtt atctttgcca gttcgattac tgcagcgcct gcggctattc ttcagttttt
    52921 gagtgccaca ggtcatgatt gggcttgggt aagggtagca caagagatgt tggcaactac
    52981 ttctccaact ggtattgcca tgtatgcttt gttgattatt ctctttacat tcttctatac
    53041 gtttgtacag attaatcctg aaaaagcagc agagagccta caaaagagtg gtgcctatat
    53101 ccatggagtt cgtcctggta aaggtacaga agaatatatg tctaaacttc ttcgtcgtct
    53161 tgcaactgtt ggttccctct tccttggtgt gatttccatt ttaccgattg cagctaaaga
    53221 tgtatttggt ctttctgatg ttgttgcctt tggtggaaca agtctcttga tcattatctc
    53281 tacaggtatc gaaggaatca agcaattgga aggttaccta ttgaaacgta agtatgttgg
    53341 tttcatggac agaacagaat aaaagtattt actgaatcag taaatactga gggagtggag
    53401 gtttaaactc tgacatttgt aagagttgga tctcccctct tctattttgt ttttaaatag
    53461 gggtgaaaag actttttgct tctatttaaa aataaaataa ggagatcaaa tcatgaatct
    53521 tttgattatg ggcttacctg gtgcaggtaa gggaactcaa gcagcaaaaa tcgtagaaca
    53581 attccatgtt gcacatatct caacaggtga tatgttccgc gctgcaatgg caaatcaaac
    53641 tgaaatgggt gttcttgcta agtcatatat tgacaagggt gaattggttc ctgacgaagt
    53701 tacaaatgga atcgtaaaag aacgcctttc acaagatgat attaaagaaa caggattctt
    53761 attggatggt tacccacgta caattgaaca agctcatgcc ttggacaaaa cattggctga
    53821 acttggcatt gaactagaag gtattatcaa tattgaagtg aaccctgaca gcctcttgga
    53881 acgtttgagt ggccgtatca tccaccgcgt aactggagaa actttccaca aggtctttaa
    53941 cccaccagtt gactataaag aagaagatta ctaccaacgt gaagatgata agcctgagac
    54001 agtaaaacgt cgtttggatg ttaatattgc tcaaggagaa ccaatcattg ctcactaccg
    54061 tgccaaaggt ttggttcatg acatcgaagg taatcaagat atcaatgatg tcttctcaga
    54121 tattgaaaaa gtattgacaa atttgaaata aagcgttttt cacacttgca aaaatccgct
    54181 acaaatgtta tactgaaata gtctgactta taattgttgt ctctgtgtct agaggcatcg
    54241 aatcgaaatt tatggaggtg cttttgcgtg gcaaaagacg atgtgattga agttgaaggc
    54301 aaagtagttg atacaatgcc gaatgcaatg tttacggttg aacttgaaaa tggacatcag
    54361 attttagcaa cagtttctgg taaaattcgt aaaaactata ttcgtatttt agcgggagat
    54421 cgtgttactg tcgaaatgag tccatatgac ttgacacgtg gacgtatcac ttaccgcttt
    54481 aaataatcga aaaacttgga gggataagaa atgaaagtaa gaccatcggt caaaccaatt
    54541 tgcgaatact gtaaagttat tcgtcgtaat ggtcgtgtta tggtaatttg cccagcaaat
    54601 ccaaaacaca aacaacgtca aggataagat agaaaggaga aaacatggct cgtattgctg
    54661 gagttgatat tccaaatgac aaacgcgtag taatctcatt gacttatgtt tatggtatcg
    54721 gacttgcaac atctaagaaa attttggctg ctgctggaat ctcagaagat gttcgtgtac
    54781 gtgatcttac atcagatcaa gaagatgcta tccgtcgtga agtggatgca atcaaagttg
    54841 aaggtgacct tcgtcgtgaa gtaaacttga acatcaaacg tttgatggaa atcggttcat
    54901 accgtggtat ccgtcaccgt cgtggacttc ctgtccgtgg acaaaatact aaaaacaacg
    54961 ctcgcactcg taaaggtaaa gctgttgcga ttgctggtaa gaaaaaataa tataggaggt
    55021 aaaagtcttg gctaaaccaa cacgtaaacg tcgtgtgaaa aagaatatcg aatctggtat
    55081 tgctcatatt cacgctacat ttaataacac tattgttatg attactgatg tgcatggtaa
    55141 tgcaattgct tggtcatcag ctggtgctct tggtttcaaa ggttctcgta aatctacacc
    55201 attcgctgct caaatggctt ctgaagctgc tgctaaatct gcacaagaac acggtcttaa
    55261 atcagttgaa gttactgtaa aaggtccagg ttctggtcgt gagtcagcta ttcgtgcgct
    55321 tgctgccgct ggtcttgaag taacagcaat tcgtgatgtg actccagtgc cacacaatgg
    55381 tgctcgtcct ccaaaacgtc gccgtgtata atcatcgcat tacactgctt ttcgtttaag
    55441 agggagtaac taaatgatcg agtttgaaaa accaaatata acaaaaattg atgaaaataa
    55501 agattatggc aagtttgtaa tcgaaccact tgaacgtggc tacggtacaa ctcttggtaa
    55561 ctctcttcgt cgtgtacttc tagcttctct accaggagca gctgtgacat ctatcaacat
    55621 tgatggtgtg ttacatgagt ttgacacagt tccaggtgtt cgtgaagacg tgatgcaaat
    55681 cattctgaac attaaaggaa ttgcagtgaa atcgtacgtt gaagacgaaa aaatcatcga
    55741 actggatgtt gaaggtcctg ctgaagtaac agctggtgac attttgacag atagcgatat
    55801 tgaaattgta aatccagatc attatctctt tacaatcggt gaaggttctt ctctaaaagc
    55861 gactatgact gttaacagtg gtcgtggata tgtacctgct gatgaaaata aaaaggataa
    55921 tgcaccagtt ggaacacttg ctgtagattc tatttataca ccagttacaa aagtcaacta
    55981 tcaagtggaa cctgctcgtg taggtagcaa tgatggtttc gacaaattaa cccttgaaat
    56041 cttgacaaat ggaacaatta ttccagaaga tgctttaggg ctttcagcac gtattttgac
    56101 agaacatctt gatttgttta caaatcttac tgagattgct aagtcaactg aagtgatgaa
    56161 agaagctgat actgaatctg acgaccgtat tttagatcgt acgattgagg aactggactt
    56221 gtctgtgcgt tcatacaact gtttaaaacg tgccggtatc aatactgtgc atgatttgac
    56281 agaaaaatct gaagcagaga tgatgaaagt acgaaatctt ggacgcaaga gtttggaaga
    56341 agtgaaactc aaactcattg atttgggtct tggattaaaa gataaataaa ggaggaatac
    56401 atggcttacc gtaaactagg acgcactagc tcacaacgta aagcaatgct tcgcgatttg
    56461 acaactgacc ttttgatcaa cgaatcaatc gtgacaactg aagctcgtgc taaagaaatc
    56521 cgtaaaactg ttgaaaaaat gattactcta ggtaaacgtg gtgatttgca tgcacgtcgt
    56581 caagcagctg ctttcgtacg taatgaaatc gcatctgaaa actatgatga agcaactgat
    56641 aagtacactt ctactacagc acttcaaaaa ttgttctcag aaatcgcacc tcgttatgct
    56701 gaacgtaacg gtggatacac tcgtatcctt aaaactgaac cacgtcgtgg tgatgcagcg
    56761 ccaatggcga tcatcgaatt agtataaaat catcaatttt gttgagtgtt atgatgatgg
    56821 agtcttgtgc tcttagtcta gctctggtct accgctagga tttcggtcct agcgggaaca
    56881 ctcatcataa gttgggatag tagacgcttg tttacgaaat tgtttttttc ttaagaacaa
    56941 cttcgtaagc aggcgttttt gagtattttc gttagaatta tgctatacta tttgaaaaga
    57001 atcctgttta atgttaaggt ttcttatttt aagaagaatt ggagtttact tatgaaagcg
    57061 attataactg ttgttggtaa agataaatct ggaattgttg caggtgtttc tggtaaaatt
    57121 gcagaattag gattgaatat tgacgatatc tctcaaactg tattggatga atattttacg
    57181 atgatggctg ttgtatctag tgatgaaaag caagatttta cctatcttcg taatgaattt
    57241 gaagcttttg ggcaaacttt gaatgtaaaa atcaatattc agagtgcagc gattttcgaa
    57301 gctatgtata atatctagga ggtcatcatg gatattagac aagttactga aaccatcgcc
    57361 atgattgagg agcaaaactt cgatattaga accattacca tggggatttc tcttttggac
    57421 tgtatcgatc cagatatcaa tcgtgctgcg gagaaaatct atcaaaaaat tacgacaaag
    57481 gcggctaatt tagtagctgt tggtgatgaa attgcggctg agttgggaat tcctatcgtt
    57541 aataagcgtg tatcggtgac acctatttct ctgattgggg cagcgacaga tgcgacggac
    57601 tacgtggttc tggcaaaagc gcttgataag gctgcgaaag agattggtgt ggactttatt
    57661 ggtggttttt ctgccttagt acaaaaaggt tatcaaaagg gagatgagat tctcatcaat
    57721 tccattcctc gcgctttggc tgagacggat aaggtctgct cgtcagtcaa tatcggctca
    57781 accaagtctg gtattaatat gacggctgtg gcagatatgg gacgaattat caaggaaaca
    57841 gcaaatcttt cagatatggg agtggccaag ttggttgtat tcgctaatgc tgttgaggac
    57901 aatccattta tggcgggtgc ctttcatggt gttggggaag cagatgttat catcaatgtc
    57961 ggagtttctg gtcctggtgt tgtgaaacgt gctttggaaa aagttcgtgg acagagcttt
    58021 gatgtagtag ccgaaacagt taagaaaact gcctttaaaa tcactcgtat cggtcaattg
    58081 gttggtcaaa tggccagtga gagactgggt gtggagtttg gtattgtgga cttgagtttg
    58141 gcaccaaccc ctgcggttgg agactctgtg gcacgtgtcc ttgaggaaat ggggctagaa
    58201 acagttggca cgcatggaac gacggctgcc ttggccctct tgaacgacca agttaaaaag
    58261 ggtggagtga tggcctgcaa ccaagtcggt ggtttatctg gtgcctttat ccctgtttct
    58321 gaggatgaag gaatgattgc tgcagtgcaa aatggctctc ttaatttaga aaaactagaa
    58381 gctatgacgg ctatctgttc tgttggattg gatatgattg ccatcccaga agatacgcct
    58441 gctgaaacca ttgcggctat gattgcggat gaagcagcaa tcggtgttat caacatgaaa
    58501 acaacagctg ttcgtatcat tcccaaagga aaagaaggcg atatgattga gtttggtggt
    58561 ctattaggaa ctgcacccgt tatgagggtt aatggggctt cgtctgtcga cttcatctct
    58621 cgcggtggac aaatcccagc accaattcat agttttaaaa attaagaaaa taggagaaat
    58681 tttaagttct atttaaga
//
LOCUS       DAVLVP010000003        57974 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.003, whole genome shotgun sequence.
ACCESSION   DAVLVP010000003 DAVLVP010000000
VERSION     DAVLVP010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 57974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 57974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..57974
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.003"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..627)
                     /locus_tag="VYN69_000146"
     CDS             complement(<1..627)
                     /locus_tag="VYN69_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HEV1532506.1"
                     /translation="MKPFQTLDEQIKLLQSRGLEIDNIEECKRYLLTNNYYNVVNGYS
                     KFFQTSKDKFITGADFREIAATHFYDKEIKSAFLKAIIDAEKHFKSVLAYCFSEAYPT
                     PYAYLDINNFETQKDTKRLAQITNLISILARILNDYNRDKQNNSIKHHYKQHGVVPFW
                     VIINELTLGQAFNFYRNLNTDIKNQIARDLSPFLQENIEYIQNRPSKDL"
     gene            complement(827..1495)
                     /locus_tag="VYN69_000147"
     CDS             complement(827..1495)
                     /locus_tag="VYN69_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HEV1532507.1"
                     /translation="MNYAQDYLDDILVRIAYHSSGIEGNTISLPETVSIILESTLPRN
                     GKSIREFYEIENHKQAFSYLLNSLANHQALTVGLVQDFHALLVDRLQHDRGQFKQVQN
                     AIIGVEFQTASPAETPYLMTQWADNTAYRLNHAQNEKEILEILADTHIQIEGIHPFSD
                     GNGRTGRLLLMYLAMKYLNAPIIVSKDSRAHYMELLANQDVTGLADLFKESLDYETTR
                     KGQF"
     gene            complement(1583..2122)
                     /locus_tag="VYN69_000148"
     CDS             complement(1583..2122)
                     /locus_tag="VYN69_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532508.1"
                     /translation="MKKLLTTSAILLTATVLVACSNNQSATKDSSEQPKTEQKNTTST
                     NTKAKVDNSKYDDLISEIKLKLDPESTGAISVKIQNNVIDSDSSEPHDAIMILLTGTA
                     KDSAKETMDAINSNSATTNQQNAITVFRMSISEFAKKLPDDNTTLSLGYEKSADQYDL
                     IAKSSKQKDFIPIGELIVN"
     gene            complement(2171..2350)
                     /locus_tag="VYN69_000149"
     CDS             complement(2171..2350)
                     /locus_tag="VYN69_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4177 domain-containing protein"
                     /protein_id="HEV1532509.1"
                     /translation="MKEKFFGTGSGNLTELEDVINKQASKGYRLHTITTANGGSKGLG
                     GGDRIQATMVFEKIL"
     gene            complement(2383..2928)
                     /locus_tag="VYN69_000150"
     CDS             complement(2383..2928)
                     /locus_tag="VYN69_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1532510.1"
                     /translation="MKIKLKELREKEKISLNKLKNILKEEYDITVSDSQLMYYENEIR
                     KPRDENIWSALAEHFGVSVAYLLGHEDEQNILKIIQSNEFKKLLNDIDIKKINELSSA
                     YKNVEEHINNPVKYNNFGKGLLNHIPSYMFTIEELINTDKENNTNFADILINYISLND
                     YDKKIAFNLVKKLSERNDEKE"
     gene            3098..3286
                     /locus_tag="VYN69_000151"
     CDS             3098..3286
                     /locus_tag="VYN69_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1532511.1"
                     /translation="MLITQEQAKALRRKKADLQLKNYELALEIGVASRTVPKILKGDY
                     KAPKRIYASVMEWLAKDY"
     gene            3525..4130
                     /locus_tag="VYN69_000152"
     CDS             3525..4130
                     /locus_tag="VYN69_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEV1532512.1"
                     /translation="MELVYMDGKKEPYTTSEIIAECAEVTHHTIQELLRKHKADFENY
                     GIIAFEMRKLDGRGRPMKIYRLNEQQATLLITYLKNTEPVRQFKMNLVKAFFEMRDEL
                     SKRYLQRELEKPKRKSLTEAIQTWEKAPKHAYSTLTNLLLKGATGKNKSQLMQERESK
                     NGIDGLTSVELTNYQRLEDMAIAMINLNRGYSEIKELIFKA"
     gene            4142..4402
                     /locus_tag="VYN69_000153"
     CDS             4142..4402
                     /locus_tag="VYN69_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532513.1"
                     /translation="MENEFKTVTNAKGLEIPKYPKDFKKLVEKDRQLAEYLCMNYEDL
                     ESEDLGAFLETVEQGFSWILDLIESKDLLYKPKSGSNHAKRK"
     gene            4739..4939
                     /locus_tag="VYN69_000154"
     CDS             4739..4939
                     /locus_tag="VYN69_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532514.1"
                     /translation="MTLDLDNMTRSEFDKLMTKIKDRNPNLFQFIIDFLDDKVSTEEV
                     YDFLKMERSYQVNHIKNYKARA"
     gene            4941..5156
                     /locus_tag="VYN69_000155"
     CDS             4941..5156
                     /locus_tag="VYN69_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532515.1"
                     /translation="MNELDLSNTQAIFVTVILIGILLYLNHRDRKKSAQIERENQQTI
                     ETPSEDLNPDYGRYIQLAGVRVSGGMK"
     gene            5153..5447
                     /locus_tag="VYN69_000156"
                     /pseudo
     CDS             5153..5447
                     /locus_tag="VYN69_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000122738.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            5428..5769
                     /locus_tag="VYN69_000157"
     CDS             5428..5769
                     /locus_tag="VYN69_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1532516.1"
                     /translation="MGLSENDKRVLRLIKVGAENSITGLEISLTTKLTERTVQDIIKR
                     LIIKHNIPIVEVRNGFYRGYFIPRNKGELLDGTKAFYNQVQEESKRLAVLMNSDLESY
                     KETLKEVGGYV"
     gene            5762..6043
                     /locus_tag="VYN69_000158"
     CDS             5762..6043
                     /locus_tag="VYN69_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532517.1"
                     /translation="MFSLSRESEKDLTHGILEVVERYLEAHEKVPPRLTKLINRIELK
                     EELKISDNTLNKWESQGLRRYQPPDDGSRMIYYLVTDIWKFLGVCDGKD"
     gene            6030..6893
                     /locus_tag="VYN69_000159"
     CDS             6030..6893
                     /locus_tag="VYN69_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conserved phage C-terminal domain-containing
                     protein"
                     /protein_id="HEV1532518.1"
                     /translation="MAKTKIYFWLKVDKKFFDNLFIKRLKNMPGGYTMIVIYIRLMLE
                     SLEDDCILYYEGYFDNLVQELALKLDVSEDDINMTVAYFTKCGLIQIDDDGHATLSQA
                     KAMVESETNWAKYKREQRKQAQNMARLESVQDSGTDSNSCPTEIEIDKESDKDKELYK
                     EYILSGKPDFTFPNWLTPRMIEEITKGHPEKYLIRIPLAYLNHTVGKNYKYLDKNLKP
                     IMARFKEGYTLEDFKQVIDIKTAEWKDSPEFSKYLRPETLFGTKFDGYLNQKPKTIKG
                     KSEDNFPDLPF"
     gene            6905..7750
                     /locus_tag="VYN69_000160"
     CDS             6905..7750
                     /locus_tag="VYN69_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1532519.1"
                     /translation="MKEQFKEFNNRKISDKFCNIHQVNYWEISIPVVGSSERKIQPFC
                     PECVKGEIKQQEQDLLQQFEDRQAYFKTYDVLMRDSTIPNELKGATFDNFFVKTTEEG
                     QMLEFVKGQAQKYLAGMTGNTLISGSTGIGKSHLSLALAKEINESFREKNDPKSVLFV
                     SLTEIIKQIKEGWAYGRNANLTEYEAVKKLVDVDFLIIDDLGAKNGTVTPKSDWEQDF
                     LFDIINNRETTIFNTNLDSSELRTVYNARNSSRILKGLEGNAFKAFTIKDKRYTINTV
                     RGEKG"
     gene            7756..7911
                     /locus_tag="VYN69_000161"
     CDS             7756..7911
                     /locus_tag="VYN69_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532520.1"
                     /translation="MDEIKFSTEKGFIVYEKCGIIEIEKIPRFGEITLVYSDGKFTHL
                     VKKETKK"
     gene            8001..8231
                     /locus_tag="VYN69_000162"
     CDS             8001..8231
                     /locus_tag="VYN69_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000012237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532521.1"
                     /translation="MSGDTSLGYVVANKFSMDPDKRQKIFSQCKKEGDSLEQRKQEIL
                     EKYANKQDKPKSRKNDSKGSESHKRKAKSKEF"
     gene            8281..9141
                     /locus_tag="VYN69_000163"
     CDS             8281..9141
                     /locus_tag="VYN69_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEV1532522.1"
                     /translation="MTTNLVKQKENLESYIRSTGYNTRGMNVENNHVLIEKPILDSYE
                     DEHQRKELVDLVNVIETRTRGGKYEVTDFESDSLQEISENFVERTESDKKKTISVDYL
                     VKLFSGKLDFSQEQLDDGQYNLTDFLGKKIIKLKRRTRNREIGKILQTAKVQTATSLD
                     DLKSIVSLINPERNVSIVLSQSLFSVLEKMKDTSGNYLLKVDKETGTSETFFVDNFLI
                     VDDTTLGNEGDKKGFIGDLENFVTLFDRKKDTLSWVNANDYFRKRLILHTRFDVKKVE
                     EDCGYFIQWN"
     gene            9151..9699
                     /locus_tag="VYN69_000164"
     CDS             9151..9699
                     /locus_tag="VYN69_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532523.1"
                     /translation="MDINQVFETLDDLDNKKSKINSAREQLSEKKKSLLGNQTVSFEN
                     INSFLSNNLESLEQLEKMEKAINSLQEKYNSDFSEAKAVIFEYIFKETKQRMETKKIY
                     KQYRKKLRRILDAYDEIQELKKDVEEIHTGVVREISQKHSLSLYRTEVSPLTVLPFLN
                     PDISGWMDFSKEYRDIKEYLEK"
     gene            10077..10487
                     /locus_tag="VYN69_000165"
     CDS             10077..10487
                     /locus_tag="VYN69_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF722 domain-containing protein"
                     /protein_id="HEV1532524.1"
                     /translation="MYELSNRDLDGVDIELGRYRTLANKIYLRRQELIHNKKHSAEDC
                     TGGKGKTVYSPTEATIIRIEEDQTLRYLEGFKLVVDTLMENLIESDLVIFKMRYLEAG
                     ATWEDVAEKLNKTTRYINSRRKVIAKRFVELKGY"
     gene            complement(11611..12453)
                     /locus_tag="VYN69_000166"
     CDS             complement(11611..12453)
                     /locus_tag="VYN69_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001065636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1532525.1"
                     /translation="MNNSIKLKRRLTQSLTYLYLIGLSIVIIYPLLITIMSAFKAGNV
                     SAFKLDTNIDLNFDNFKGLFTETLYGTWYLNTLIIALITMAVQTSIIVLAGYAYSRYN
                     FLARKQSLVFFLIIQMVPTMAALTAFFVMALMLNALNHNWFLIFLYVGGGIPMNAWLM
                     KGYFDTVPMSLDESAKLDGAGHFRRFWQIVLPLVRPMVAVQALWAFMGPFGDYILSSF
                     LLREKEYFTVAVGLQTFVNNAKNLKIAYFSAGAILIALPICILFFFLQKNFVSGLTSG
                     GDKG"
     gene            complement(12455..13747)
                     /locus_tag="VYN69_000167"
     CDS             complement(12455..13747)
                     /locus_tag="VYN69_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1532526.1"
                     /translation="MEKQQPSKAALLSIIPGLGQIYNKQKAKGFIFLGVTIVFVLYFL
                     ALATPELSNLITLGDKPGRDNSLFMLIRGAFHLIFVIVYVLFYFSNIKDAHTIAKRIN
                     NGIPVPRTLKDMIKGIYENGFPYLLIIPSYVAMTFAIIFPVIVTLMIAFTNYDFQHLP
                     PNKLLDWVGLTNFTNIWSLSTFRSAFGSVLSWTIIWALAASTLQIVIGIFTAIIANQP
                     FIKGKRIFGVIFLLPWAVPAFITILTFSNMFNDSVGAINTQVLPILAKFLPFLDGALI
                     PWKTDPTWTKIALIMMQGWLGFPYIYVLTLGILQSIPNDLYEAAYIDGANAWQKFRNI
                     TFPMILAVAAPTLISQYTFNFNNFSIMYLFNGGGPGSVGGGAGSTDILISWIYRLTTG
                     TSPQYSMAAAVTLIISIIVISISMIAFKKLHAFDMEDV"
     gene            complement(13858..15129)
                     /locus_tag="VYN69_000168"
     CDS             complement(13858..15129)
                     /locus_tag="VYN69_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1532527.1"
                     /translation="MSSKFMKSTAVLGTVTLASLLLVACGSKTADKPADSGSSEVKEL
                     TVYVDEGYKSYIEEVAKAYEKEAGVKITLKTGDALGGLDKLSLDNQSGNVPDVMMAPY
                     DRVGSLGSDGQLSEVKLSDGAKTDDTTKSLVTAANGKVYGAPAVIESLVMYYNKDLVK
                     DAPKTFADLENLAKDSKYAFAGEDGKTTAFLADWTNFYYTYGLLAGNGAYVFGQNGKD
                     AKDIGLANDGSIVGINYAKSWYEKWPKGMQDTEGAGNLIQTQFQEGKTAAIIDGPWKA
                     QAFKDAKVNYGVATIPTLPNGKEYAAFGGGKAWVIPQAVKNLEASQKFVDFLVATEQQ
                     KVLYDKTNEIPANTEARSYAEGKNDELTTAVIKQFKNTQPLPNISQMSAVWDPAKNML
                     FDAVSGQKDAKTAANDAVTLIKETIKQKFGE"
     gene            15679..17196
                     /gene="malQ"
                     /locus_tag="VYN69_000169"
     CDS             15679..17196
                     /gene="malQ"
                     /locus_tag="VYN69_000169"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HEV1532528.1"
                     /translation="MKKRQSGVLMHISSLPGAYGIGSFGQSAYDFVDFLVRTKQRYWQ
                     ILPLGATSYGDSPYQSFSAFAGNTHFIDLDILVEQGLLGASDLEGVDFGSDASEVDYA
                     KIYYARRPLLEKAVKRFFEVGDVKDFEKFAQDNQSWLELFAEYMAIKEHFDNLAWTEW
                     PDADARARKASALESYREQLADKLVYHRVTQYFFFQQWLKLKAYANDNHIEIVGDMPI
                     YVAEDSSDMWANPHLFKTDVNGKATCIAGCPPDEFSVTGQLWGNPIYDWEAMDKDGYK
                     WWIERLRESFKIYDIVRIDHFRGFESYWEIPAGSDTAAPGEWVKGPGYKLFAAVKEEL
                     GELNIIAEDLGFMTDEVIELRERTGFPGMKILQFAFNPEDESIDSPHLAPANSVMYTG
                     THDNNTVLGWYRNEIDDATREYMARYTNRKEYETVVHAMLRTVFSSVSFMAIATMQDL
                     LELDEAARMNFPSTLGGNWSWRMTEDQLTPAVEEGLLDLTTIYRRINENLVDLKK"
     gene            17222..19480
                     /gene="glgP"
                     /locus_tag="VYN69_000170"
     CDS             17222..19480
                     /gene="glgP"
                     /locus_tag="VYN69_000170"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000950172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan family
                     phosphorylase"
                     /protein_id="HEV1532529.1"
                     /translation="MLSLQEFVQNRYNKTIAECSNEELYLALLNYSKLASSQKPVNTG
                     KKKVYYISAEFLIGKLLSNNLINLGLYDDVKKELAAAGKDLIEVEEVELEPSLGNGGL
                     GRLAACFIDSIATLGLNGDGVGLNYHFGLFQQVLKNNQQETIPNAWLTEQNWLVRSSR
                     SYQVPFADFTLTSTLYDIDVTGYETATKNRLRLFDLDSVDSSIIKDGINFDKTDIARN
                     LTLFLYPDDSDRQGELLRIFQQYFMVSNGAQLIIDEAIEKGSNLHDLADYAVVQINDT
                     HPSMVIPELIRLLTARGIELDEAISIVRSMTAYTNHTILAEALEKWPLEFLQEVVPHL
                     VPIIEELDRRVKAEYKDPAVQIIDESGRVHMAHMDIHYGYSVNGVAALHTEILKNSEL
                     KAFYDLYPEKFNNKTNGITFRRWLMHANPRLSHYLDEILGDGWHHEADELEKLLSYED
                     KAAVKEKLESIKAHNKRKLARHLKEHQGVEINPNSIFDIQIKRLHEYKRQQMNALYVI
                     HKYLDIKAGNIPARPITIFFGGKAAPAYTIAQDIIHLILCMSEVIANDPAVAPHLQVV
                     MVENYNVTAASFLIPACDISEQISLASKEASGTGNMKFMLNGALTLGTMDGANVEIAE
                     LVGDENIYIFGEDSETVIDLYAKAAYKSSEFYAREAIKPLVDFIVSDAVLAAGNKERL
                     ERLYNELINKDWFMTLLDLEDYIKVKEQMLADYEDRDAWLDKVIVNISKAGFFSSDRT
                     IAQYNEDIWHLN"
     gene            complement(20524..21096)
                     /locus_tag="VYN69_000171"
     CDS             complement(20524..21096)
                     /locus_tag="VYN69_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532530.1"
                     /translation="MNKLMKFISVFLTSIVLIVSAIPSVSAVYASEQVSQIETNMELQ
                     PVTSLTEEQINTLANEIQSFHPDVSQQWIKEVINRQLQGDYTIPPTYSPFRAAWQGIT
                     VNQMGALLDTAIALALGGTTAGLANLIKVKGKHAAKSAIRSAISRYLGSWFVNDVALE
                     SAMNLLSPGTYLTQLWDKNDAIPNNGRINF"
     gene            complement(21718..22566)
                     /locus_tag="VYN69_000172"
     CDS             complement(21718..22566)
                     /locus_tag="VYN69_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1532531.1"
                     /translation="MNTNLKPKLQRFASATTFACPICQENLTLLETNFKCCNRHSFDL
                     AKFGYVNLAPQIKQSANYDKENFQNRQQILEAGFYQAILDAVSDLLASSKTTTTILDI
                     GCGEGFYSRKLQESHSEKTFYAFDISKDSVQIAAKSEPNWAVNWFVGDLARLPIKDAN
                     MDILLDIFSPANYGEFRRVLSKDGILIKVIPTENHLKEIRQRVQDQLTNKEYSNQDIK
                     EHFQEHFTILSSQTASLTKTITAEQLQALLSMTPLLFHVDQSKIDWSQLTEITIEAEI
                     LVGKAF"
     gene            22718..22996
                     /locus_tag="VYN69_000173"
     CDS             22718..22996
                     /locus_tag="VYN69_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003075836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532532.1"
                     /translation="MLKEVLTVAKVAKKSSLFLGGVAFGTLGLKILASKEAKKGYSKA
                     LAKAYKLKDELDASVSVVKQHGDDVLQDAKYLYEQEKKEEQLDSLIGE"
     gene            22998..25061
                     /locus_tag="VYN69_000174"
     CDS             22998..25061
                     /locus_tag="VYN69_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000008803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1532533.1"
                     /translation="MSFKVLHRGYQHIRLSSSFSLTLDIQDYLRSLARDEKGIESIQF
                     YMDQQHFTLRIKEGFSVLDNAEAFLKRIDKGKVSELMTLPIRREESAYSIVSGAAVKR
                     VLFRSFVPYPIRYIWTCYQALGYIREAYQTLARKELTMEVLDCSAILLSLFMNQSKTA
                     SNIMFMLDLGNHLDQWSLKKTATDLEQSLLAKESDVFLVQGDTVVSIKSSDVQIGDVL
                     ILSQGNEILFDGQVVSGLGMVNESSLTGESFPVEKRESDLVCANTVLETGELRIRVTD
                     NQMNSRILQLIELMKKSEENKKTKQRYFIKMADKVVKYNFLGAGLTYLLTGSFSKAIS
                     FLLIDFSCALKISTPVAYLTVIKEGLNREMVIKDGDVLEKYLEVDTFLFDKTGTITTS
                     YPIVEKVLPFGDYSEEDILRISACLEEHIYHPIANAIVKQAEIEGIEHEEMHGKLQYI
                     ASKGIKSHIDGQPVLIGNYVLMQDEQIHISSEQNALIEEYKSHYNLLFLAYQNELIGM
                     FCIHTPLRKEAKTALDKLKAQGKKLILATGDTLIRTEELVKDLPFDQVYTDLKPDGKF
                     ELVEKLQKAGHTILMVGDGLNDSAALTLSDIGVVMNESADISKQMSDILLLDNRLDFF
                     QELDSLSSSLQTLIKKNIQDTVVVNSSLIGFGLFNWFSPSNLSILHNLTTLRIVLRSL
                     SIKNR"
     gene            complement(25139..26395)
                     /locus_tag="VYN69_000175"
     CDS             complement(25139..26395)
                     /locus_tag="VYN69_000175"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEV1532534.1"
                     /translation="MHIFDELKERGLIFQTTDEEALRKALEEGQVSYYTGYDPTADSL
                     HLGHLVAILTSRRLQLAGHKPYALVGGATGLIGDPSFKDAERSLQTKDTVDGWVKSIQ
                     GQLSRFLDFENGENKAVMVNNYDWFGSISFIDFLRDIGKYFTVNYMMSKESVKKRIET
                     GISYTEFAYQIMQGYDFFVLNQDHNVTLQIGGSDQWGNMTAGTELLRRKADKTGHVIT
                     VPLITDATGKKFGKSEGNAVWLNPEKTSPYEMYQFWMNVMDADAVRFLKIFTFLSLDE
                     IEDIRKQFEAAPHERLAQKVLAREVVTLVHGEEAYKEALNITEQLFAGNIKNLSVKEL
                     KQGLRGVPNYQVQADEHNNIVELLVSSGIVNSKRQAREDVQNGAIYVNGDRIQDLDYV
                     LSDADKLENELTVIRRGKKKYFVLTY"
     gene            26537..29002
                     /locus_tag="VYN69_000176"
     CDS             26537..29002
                     /locus_tag="VYN69_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1532535.1"
                     /translation="MQNQLNELKRKMLEFFQQKQKNKKSSRPGKKGSSTKKSKTLDKS
                     AIFPAILLSIKALFNLLFVLGFLGGMLGAGIALGYGVALFDKVRVPQTEELVNQVKDI
                     SSISEITYSDGTVIASIESDLLRTSISSEQISENLKKAIIATEDEHFKEHKGVVPKAV
                     IRATLGKFVGLGSSSGGSTLTQQLIKQQVVGDAPTLARKAAEIVDALALERAMNKDEI
                     LTTYLNVAPFGRNNKGQNIAGARQAAEGIFGVDASQLTVPQAAFLAGLPQSPITYSPY
                     ENTGELKSDEDLEIGLRRAKAVLYSMYRTGALSKDEYSQYKDYDLKQDFLPSGTVTGI
                     SRDYLYFTTLAEAQERMYDYLAQRDNVSAKELKNEATQKFYRDLAAKEIENGGYKITT
                     TIDQKIHSAMQSAVADYGYLLDDGTGRVEVGNVLMDNQTGAILGFVGGRNYQENQNNH
                     AFDTKRSPASTTKPLLAYGIAIDQGLMGSETILSNYPTNFANGNPIMYANSKGTGMMT
                     LGEALNYSWNIPAYWTYRMLREKGVDVKGYMEKMGYEIPEYGIESLPMGGGIEVTVAQ
                     HTNGYQTLANNGVYHQKHVISKIEAADGRVVYEYQDKPVQVYSKATATIMQGLLREVL
                     SSRVTTTFKSNLTSLNPTLANADWIGKTGTTNQDENMWLMLSTPRLTLGGWIGHDDNH
                     SLSRRAGYSNNSNYMAHLVNAIQQASPSIWGNERFALDPSVVKSEVLKSTGQKPGKVS
                     VEGKEVEVTGSTVTSYWANKSGAPATSYRFAIGGSDADYQNAWSSIVGSLPTPSSSSS
                     SSSSSSDSSNSSTTRPSSSRARR"
     gene            29067..29978
                     /locus_tag="VYN69_000177"
     CDS             29067..29978
                     /locus_tag="VYN69_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1532536.1"
                     /translation="MNQYQKKIVNGKIYSLLSGLIWGICGILGEYFFTHYQVSSGWIT
                     SMRLTLAGSLVLIWSAIQLKSQVLDIWRDKKNYLPFLAYAILGIFSVQYFFYLCVEYS
                     NATTATILQFISPVFILFYNRLVYQKRASKSAVFYVLVAMLGVCLMATKGDLSQLSMT
                     PLALITGLLSAMGVMFNVILPQPFAKRYGFVPTVGWGMILAGLFSNVLSPIYQLSFTL
                     DIWSILICLIIAFFGTAFAFFISMKAVSLVSPLVVSVISASEPLSSALLSVLFLGLVV
                     DWSLLLAIALIILPMIFLSIEEAKESR"
     gene            30208..30906
                     /gene="dapD"
                     /locus_tag="VYN69_000178"
     CDS             30208..30906
                     /gene="dapD"
                     /locus_tag="VYN69_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEV1532537.1"
                     /translation="MTATKMNAQEIIQFIANAEKKTSVKVTFEGQLATAVPSSVVKLG
                     NVLFGDWKDVAPLLDGLVENQDYVVEQDARNSAVPLLDKRAINARIEPGAIIRDQVEI
                     GDNAVIMMGAVINIGAEIGAGTMIDMGAILGGRAIVGKNSHVGAGAVLAGVIEPASAE
                     PVRVGDNVLIGANAVVIEGVQIGSGSVVAAGAIVTQDVPENVVVAGVPARIIKEIDAQ
                     TQQKTALEDALRTL"
     gene            30974..32104
                     /locus_tag="VYN69_000179"
     CDS             30974..32104
                     /locus_tag="VYN69_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyldiaminopimelate deacetylase"
                     /protein_id="HEV1532538.1"
                     /translation="MLDLIQTRRDLHQIPEIGLEEFKTQAYLLDVIEKLTTGKDFVQI
                     RTWRTGILVYLQGSQPERTIGWRTDIDGLPIVEQTGLLFASQHQGRMHACGHDFHMTI
                     ALGCLERALEEQPKNNLLFLFQPAEENEAGGMLMYEDGAFGDWLPDQFYGLHVRPDLK
                     VGQIATNTHTLFAGTCEVKIRFKGKGGHAAFPHEANDALVVASYFVTQVQSVVSRNVN
                     PIEGAVVTFGVFQAGTTNNVITDTAFLHGTIRALTQDMSLLVQKRVKTVAEGVAAAFD
                     MEVEVELKQGGYLPVENNPALARELMDFFDEKDGIELIDIEPAMTGEDFGYLLSKVDG
                     VMFWLGIDSPYALHHPQMSPKEEVLAIGVAAVSSFLKKKAAE"
     gene            32116..32655
                     /locus_tag="VYN69_000180"
     CDS             32116..32655
                     /locus_tag="VYN69_000180"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEV1532539.1"
                     /translation="MKSELRKQVLHEMKALSQEQKQAIDQALTERILQHPFYQEAKVI
                     ATYLSFSHEFQTRELIEQALKDGKKVLIPKTYPKGRMEFVVYDPQQLVKTSFALLEPQ
                     GDLEVVDVSQIDLIHVPGLAFTTDGYRIGYGGGYYDRYLEHFSGRTLSTVYPCQIQDF
                     IPENHDIPVQEVLIDEGNL"
     gene            32639..33316
                     /locus_tag="VYN69_000181"
     CDS             32639..33316
                     /locus_tag="VYN69_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEV1532540.1"
                     /translation="MKEIFDRRYPVTSFFLLVTALVFLLMLVTAGGNFDRADTLFRFG
                     AMYGPAIRLFPEQIWRLLSAIFVHIGWEHFIVNMLSLYYLGRQVEEIFGSKQFFFLYL
                     LSGMMGNLFVFVFSPKSLAAGASTSLYGLFAAIIILRYATRNPYIQQLGQSYLTLFVV
                     NIIGSVLIPGISLAGHIGGAVGGAFLAVIFPVRGEKRMYNTSQRLGAVVLFVGLAILL
                     FYKGMGM"
     gene            complement(33383..34282)
                     /gene="galU"
                     /locus_tag="VYN69_000182"
     CDS             complement(33383..34282)
                     /gene="galU"
                     /locus_tag="VYN69_000182"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEV1532541.1"
                     /translation="MTSKVRKAVIPAAGLGTRFLPATKALAKEMLPIVDKPTIQFIVE
                     EALKSGIEDILVVTGKSKRSIEDHFDSNFELEYNLKEKGKTDLLKLVEETTGMRLHFI
                     RQTHPRGLGDAVLQAKAFVGNEPFVVMLGDDLMDITDEKAVPLTKQLMDDYERTHAST
                     IAVMPVPHDEVSAYGVIAPQGEGKDGLYSVETFVEKPAPEDAPSDLAIIGRYLLTPEI
                     FEILEKQAPGAGNEIQLTDAIDTLNKTQRVFAREFKGARYDVGDKFGFMKTSIDYALK
                     HPQVKDDLKNYLIQLGKELTEKE"
     gene            complement(34304..35320)
                     /locus_tag="VYN69_000183"
     CDS             complement(34304..35320)
                     /locus_tag="VYN69_000183"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1532542.1"
                     /translation="MEKQTVAVLGPGSWGTALSQVLNDNGHEVRIWGNLPEQINEINT
                     HHTNKHYFKDVVLDENIIAYTDLAETLKDVDAILFVVPTKVTRLVAQQVAQTLDHKVI
                     IMHASKGLEPDSHKRLSTILEEEIPEHLRSDIVVVSGPSHAEETIVRDLTLITAASKD
                     LQTAQYVQKLFSNHYFRLYTNTDVIGVETAGALKNIIAVGAGALHGLGFGDNAKAAII
                     ARGLAEITRLGVALGASPLTYSGLSGVGDLIVTGTSIHSRNWRAGDALGRGESLADIE
                     ANMGMVIEGISTTRAAYELAQELGVYMPITQAIYQVIYHGTNIKDAIYDIMNNEFKAE
                     NEWS"
     gene            35532..35912
                     /locus_tag="VYN69_000184"
     CDS             35532..35912
                     /locus_tag="VYN69_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1532543.1"
                     /translation="MREFGEKIKRLRLAKKISRSEFCGDESELSIRQLIRIENGESRP
                     TLTKLKYIAERLGVEDYKLMPSYIELDKEYLELKYFLMRTPTYEDETIAQKKESVLIR
                     FLKSIMIGYLRKKDLSSQIIHIWH"
     gene            complement(36086..36511)
                     /locus_tag="VYN69_000185"
     CDS             complement(36086..36511)
                     /locus_tag="VYN69_000185"
                     /inference="COORDINATES: protein motif:HMM:NF025080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1532544.1"
                     /translation="MTNLSSVDSEELFQFYRERGNAENFIKERKAGFFGDKTDSSTMI
                     KNEVRMMMGCLAYNLYLFLKQLAGDEVKALTIKRFRRLFLHIAGKYVSTARRHILKFS
                     SLYAYSKQFQALFDTICQINLILPVPYRARGQGKTCLTE"
     gene            complement(36579..37229)
                     /gene="phoU"
                     /locus_tag="VYN69_000186"
     CDS             complement(36579..37229)
                     /gene="phoU"
                     /locus_tag="VYN69_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1532545.1"
                     /translation="MRNQFDLELHELEQSFLGLGQLVLETASKALLALASKDKEMAEL
                     IINKDHAINQGQSAIELTCARLLALQQPQVSDLRFVISIMSSCSDLERMGDHMAGIAK
                     AVLQLKENQLAPDEEQLHQMGKLSLSMLADLLVAFPLHQASKAISIAQKDEQIDQYYY
                     ALSKEIIGLMKDQETSIPNGTQYLYIIGHLERFADYIANICERLVYLETGELVDLN"
     gene            complement(37244..37996)
                     /gene="pstB"
                     /locus_tag="VYN69_000187"
     CDS             complement(37244..37996)
                     /gene="pstB"
                     /locus_tag="VYN69_000187"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1532546.1"
                     /translation="MGTFSVRHLDLFYGDFQALKNISIQLPERQITALIGPSGCGKST
                     FLKTLNRMNDLVPSCHIEGQVLLDEQDIYSSKFNLNQLRKRVGMVFQQPNPFAMSIYD
                     NVAYGPRTHGIRDKKQLDALVEKSLKGAAIWEEVKDDLKKSAMSLSGGQQQRLCIARA
                     LAVEPDILLMDEPTSALDPISTLKIEDLIQQLKKDYTIIIVTHNMQQASRISDKTAFF
                     LTGEICEFGDTVDVFTNPKDQRTEDYISGRFG"
     gene            complement(37998..38813)
                     /gene="pstA"
                     /locus_tag="VYN69_000188"
     CDS             complement(37998..38813)
                     /gene="pstA"
                     /locus_tag="VYN69_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015057412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1532547.1"
                     /translation="MSKYLLKLLVYCFSALTFGSLFLIIGFILIKGLPHLSLSLFSWT
                     YTSENISLMPAIISTVILVFGALLLALPIGIFAGFYLVEYTKKDSLCVKIMRLASDTL
                     SGIPSIVFGLFGMLFFVVFLGFQYSLLSGILTSVIMVLPVIIRSTEEALLSVSDSMRQ
                     ASYGLGAGKLRTVFRIVLPVAMPGILAGVILAIGRIVGETAALMYTLGTSTNTPSSLM
                     SSGRSLALHMYMLSSEGLHVNEAYATGVILIITVLMINTLSSLLSRKLVKGAS"
     gene            complement(38806..39669)
                     /gene="pstC"
                     /locus_tag="VYN69_000189"
     CDS             complement(38806..39669)
                     /gene="pstC"
                     /locus_tag="VYN69_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1532548.1"
                     /translation="MTKQAFKEAVFRAIFFMSATVAVVAILLICFFIFSNGLPFMANY
                     GFARFLLGSDWSPTNIPASYGILPMIVGSLLITLGAIVIGVPTGILTSVFMVYYCPKP
                     VYGFLKSAINLMAAIPSIVYGFFGLQLLVPWIRSFLGNGMSVLTASLLLGIMILPTII
                     SLSESAIRTVPKTYYSGSLALGASHERSIFSVILPAARSGILSAVILGIGRAVGETMA
                     VILVAGNQPIIPSGLFSGTRTLTTNIVLEMAYASGQHREALIATSAVLFFLILLINAY
                     FAYLKGKSSYE"
     gene            complement(39787..40662)
                     /locus_tag="VYN69_000190"
     CDS             complement(39787..40662)
                     /locus_tag="VYN69_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-binding protein"
                     /protein_id="HEV1532549.1"
                     /translation="MKFKKMLTLAAIGLSGFGLVACGNQSAASKQSASGTIEVISREN
                     GSGTRGAFTEITGILKKDGDKKIDNTAKTAVIQNSTEGVLSAVQGNANAIGYISLGSL
                     TKSVKALEIDGVKASRDTVLDGEYPLQRPFNIVWSSNLSKLGQDFISFIHSKQGQQVV
                     TDNKFIEAKTETTEYTSQHLSGKLSVVGSTSVSSLMEKLAEAYKKENPEVTIDITSNG
                     SSAGITAVKEKTADIGMVSRELTPEEGKSLTHDAIALDGIAVVVNNDNKASQVSMAEL
                     ADVFSGKLTTWDKIK"
     gene            complement(40829..42160)
                     /locus_tag="VYN69_000191"
     CDS             complement(40829..42160)
                     /locus_tag="VYN69_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEV1532550.1"
                     /translation="MKRYLQFWLVNLSVSLILIAGMALTWISKGIGLFLLALSLGLGG
                     YWLFCLWKWEVAFETLHQPLLTSSEYFLEKGQEDLKSLAQYVSGLKTKVSQQDQQYKD
                     LAETMEVLLSHLTMGTFLVSAQGQMLLSSRSLPHYFPDVDGDISSLDDLKRMDIRNLV
                     HQAFDQKTRLKQEVSGFHEGDLILEVTAVPVFSPTQSVEAVLVLLYDLTTIRTYEKLN
                     LAFVSNASHELRTPVTSIKGFAETIKGMSAEEEALKDDFLDIIYKESLRLEHIVEHLL
                     TLSKAQQMPIQWTTLSLAEFVQDLTQSLQPQLKKKDLQLKVQVPDDVTLVSDSQLLSQ
                     ILLNLLSNAIRYTEQGGKIEVKTQKVNEGIKISVSDTGIGISQLEQDRIFERFYRVNK
                     GRSRQTGGTGLGLAIVKELSQLLGGQVTVTSQLGRGSCFTIFLPNQSFAQD"
     gene            complement(42161..42868)
                     /locus_tag="VYN69_000192"
     CDS             complement(42161..42868)
                     /locus_tag="VYN69_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1532551.1"
                     /translation="MTKQVLLVDDEEHILKLLDYHLSKEGFSTQLVTNGRKALALAET
                     EPFDFILLDIMLPQLDGIEVCKRLRAKGVKTPIMMVSAKSDEFDKVLALELGADDYLT
                     KPFSPRELLARVKAVLRRTKGEQEGDDSDNIADDSWLFGTLKVYPERHEVYKANKLLS
                     LTPKEFELLLYLMKHPNMTLTRERLLERIWGYDFGQETRLVDVHIGKLREKIEDNPKA
                     PQFIRTIRGYGYKFKEL"
     gene            complement(42952..43437)
                     /locus_tag="VYN69_000193"
     CDS             complement(42952..43437)
                     /locus_tag="VYN69_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEV1532552.1"
                     /translation="MAPTDKEDLIRTLELAPHVEGGYFRQTKKASDTINLAGKGRALY
                     TSIYFLLEETNPSHFHRLTADEIWYFHAGSPLTVHMITADGHYEAVTLGLDISKGQQL
                     HYCVPKGTIWGSTVDKDDALVSCLVAPGFEFEDFELFERVDLLATYPEHKEMIERLTR
                     Y"
     gene            43423..43980
                     /locus_tag="VYN69_000194"
                     /pseudo
     CDS             43423..43980
                     /locus_tag="VYN69_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(44295..45986)
                     /locus_tag="VYN69_000195"
     CDS             complement(44295..45986)
                     /locus_tag="VYN69_000195"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEV1532553.1"
                     /translation="MNTKELIASELSSIIDSLDQEAILKLLETPKNSEMGDIAFPAFS
                     LAKVERKAPQMIAAELAEKMNSQAFEKVVATGPYVNFFLDKSAISAQVLQAVTTEKEH
                     YADQNIGKQENVVIDMSSPNIAKPFSIGHLRSTVIGDSLSHIFQKIGYQTVKVNHLGD
                     WGKQFGMLIVAYKKWGDEEAVKAHPIDELLKLYVRINAEAENDPSLDEEAREWFRKLE
                     NGDEEALALWQWFRDESLVEFNRLYNELKVEFDSYNGEAFYNDKMDAVVDILSEKGLL
                     LESEGAQVVNLEKYGIEHPALIKKSDGATLYITRDLAAALYRKNEYQFAKSIYVVGQE
                     QSAHFKQLKAVLQEMGYDWSDDITHVPFGLVTKEGKKLSTRKGNVILLEPTVAEAVSR
                     AKVQIEAKNPELENKDQVAHAVGVGAIKFYDLKTDRTNGYDFDLEAMVSFEGETGPYV
                     QYAYARIQSILRKADFKPETAGNYSLNDTESWEIIKLIQDFPRIINRAADNFEPSIIA
                     KFAISLAQSFNKYYAHTRILDESPERDSRLALSYATAVVLKEALRLLGVEAPEKM"
     gene            46122..46568
                     /gene="argR"
                     /locus_tag="VYN69_000196"
     CDS             46122..46568
                     /gene="argR"
                     /locus_tag="VYN69_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1532554.1"
                     /translation="MRKRDRHQLIKKMITEEKLSTQKEIQDRLEAHNVCVTQTTLSRD
                     LREIGLTKVKKNDMVYYVLVNETEKIDLVEFLSHHLEGVARAEFTLVLHTKLGEASVL
                     ANIVDVNKDEWILGTVAGANTLLVICRDQHVAKLMEDRLLDLMKDK"
     gene            46619..49153
                     /gene="mutS"
                     /locus_tag="VYN69_000197"
     CDS             46619..49153
                     /gene="mutS"
                     /locus_tag="VYN69_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEV1532555.1"
                     /translation="MAIEKLSPGMQQYVDIKKQYPDAFLLFRMGDFYELFYEDAVNAA
                     QILEISLTSRNKNADNPIPMAGVPYHSAQQYIDVLIEQGYKVAIAEQMEDPKQAVGVV
                     KREVVQVITPGTVVDSSKPDSQNNFLVSIDREGNQFGLAYMDLVTGDFYVTGLLDFTL
                     VCGEIRNLKAREVVLGYDLSEEEEQILSRQMNLVLSYEKESFEDLHLLDLRLATVEQT
                     ASSKLLQYVHRTQMRELNHLKPVIRYEIKDFLQMDYATKASLDLVENARSGKKQGSLF
                     WLLDETKTAMGMRLLRSWIHRPLIDKERIVQRQEVVQVFLDHFFERSDLTDSLKGVYD
                     IERLASRVSFGKTNPKDLLQLATTLSSVPRIRAILEGMEQPTLAYLIAQLDAIPELES
                     LISAAIAPEAPHVITDGGIIRTGFDETLDKYRCVLREGTSWIAEIEAKERENSGISTL
                     KIDYNKKDGYYFHVTNSQLGNVPAHFFRKATLKNSERFGTEELARIEGDMLEAREKSA
                     NLEYEIFMRIREEVGKYIQRLQALAQGIATVDVLQSLAVVAETQHLIRPEFGDDSQID
                     IRKGRHAVVEKVMGAQTYIPNTIQMAEDTSIQLVTGPNMSGKSTYMRQLAMTAVMAQL
                     GSYVPAESAHLPIFDAIFTRIGAADDLVSGQSTFMVEMMEANNAISHATKNSLILFDE
                     LGRGTATYDGMALAQSIIEYIHEHIGAKTLFATHYHELTSLESSLQHLVNVHVATLEQ
                     DGQVTFLHKIEPGPADKSYGIHVAKIAGLPADLLARADKILTQLENQGTESPPPMRQT
                     SAVTEQISLFDRAEEHPILAELAKLDVYNMTPMQVMNVLVELKQKL"
     gene            49300..50994
                     /gene="patA"
                     /locus_tag="VYN69_000198"
     CDS             49300..50994
                     /gene="patA"
                     /locus_tag="VYN69_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatA"
                     /protein_id="HEV1532556.1"
                     /translation="MLIQKIKTYKWQALASLLMTGLMVASSLLQPRYLQEVLDALLTG
                     KYEAIYSIGAWLIGVAVVGLVAGGLNVVLAAYIAQGVSSDLREDAFRKIQTFSYADIE
                     QFNAGNLVVRMTNDINQIQNVVMMTFQILFRLPLLFIGSFILAVQTLPSLWWVIVLMV
                     VLIFGLTAVMMGMMGPRFAKFQTLLERINAIAKENLRGVRVVKSFVQEKEQFAKFTEV
                     SDELFGQNLYIGYAFSVVEPFMMLVGYGAVFLSIWLVAGMVQSDPSVVGSIASFVNYL
                     SQIIFTIVMVGFLGNSVSRAMISMRRIREILDAEPAMTFKDIPDEELVGSLSFENVTF
                     TYPMDKEPMLKDVSFTIEPGQMVGVVGATGAGKSTLAQLIPRLFDPQNGAIKIGGKDI
                     REVSEGTLRKTVSIVLQRAILFSGTIADNLRQGKGNATLFEMERAANIAQASEFIHRM
                     EKTFESPVEERGTNFSGGQKQRMSIARGIVSNPRILIFDDSTSALDAKSERLVQEALN
                     KDLKGTTTIIIAQKISSVVHADKILVLNQGRLIGQGTHADLVANNAVYREIYETQK"
     gene            complement(51325..51594)
                     /locus_tag="VYN69_000199"
                     /pseudo
     CDS             complement(51325..51594)
                     /locus_tag="VYN69_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019770457.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            51782..53548
                     /gene="patB"
                     /locus_tag="VYN69_000200"
     CDS             51782..53548
                     /gene="patB"
                     /locus_tag="VYN69_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000859866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit PatB"
                     /protein_id="HEV1532557.1"
                     /translation="MKTVQFFWHYFKVYKFSFVVVILMIVLATFAQALFPVFSGQAVT
                     QLANLVQAYQNGNPELVWQSLSGIMVNLGLLVLVLFISSVIYMCLMTRVIAESTNEMR
                     KGLFGKLAQLTVSFFDRRQDGDILSHFTSDLDNILQAFNESLIQVMSNIVLYIGLILV
                     MFSRNVTLALITIASTPLAFLMLIFIVKMARKYTNLQQKEVGKLNAYMDESISGQKAV
                     IVQGIQEDMMAGFLEQNERVRKATFKGRMFSGILFPVMNGMSLINTAIVIFAGSAVLL
                     NDKSIETSTALGLIVMFAQFSQQYYQPIIQVAASWGSLQLAFTGAERIQEMFDAEEEI
                     RPEKAPTFTKLQESVEISHIDFSYLPDKPILKDVSISAPKGQMTAVVGPTGSGKTTIM
                     NLINRFYDVDAGGIYFDGKDIRGYDLDSLRSKVGIVLQDSVLFSGTIRDNIRFGVPDA
                     SQEMVEVAAKATHIHDYIESLPDKYDTLIDDDQSIFSTGQKQLISIARTLMTDPEVLI
                     LDEATSNVDTVTESKIQHAMEVVVAGRTSFVIAHRLKTILNADQIIVLKDGEVIERGN
                     HHELLKLGGFYSELYHNQFVFE"
     gene            53687..54376
                     /locus_tag="VYN69_000201"
     CDS             53687..54376
                     /locus_tag="VYN69_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1532558.1"
                     /translation="MARTVVGVAANLCPVDAEGKIIHSSVSCRFAEIIRQVGGLPLVI
                     PVGDESVVRDYVEMIDKLILTGGQNVHPQFYGEKKTVESDDYNLVRDEFELALLKEAL
                     RQNKPIMAICRGVQLVNVAFGGTLNQEIEGHWQGLPFGTSHSIETVEGSVVAKLFGKE
                     SQVNSVHRQSIKDLAPNFRVTAIDSRDQTIEAIESIDEHRIIGLQWHPEFLVNEEDGN
                     LELFEYLLNEL"
     gene            54380..54559
                     /locus_tag="VYN69_000202"
     CDS             54380..54559
                     /locus_tag="VYN69_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532559.1"
                     /translation="MEHLVVLSFIFSKFLECGILRKRLNSDKNWRNSTKKLEKNEGKR
                     YDRKEEILEEEHVTY"
     gene            54546..55895
                     /locus_tag="VYN69_000203"
     CDS             54546..55895
                     /locus_tag="VYN69_000203"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEV1532560.1"
                     /translation="MSHIKFDYSKVLDKFVAPHEVEYMQSQVTAADELIRKGTGAGSD
                     FLGWLDLPEKYDREEFDRILKAAEQIKSDSDVLVVIGIGGSYLGAKAAIDFLNHHFAN
                     LQTKEERKAPQILYAGNSISSTYLADLVEYVADKDFSVNVISKSGTTTEPAIAFRVFK
                     ELLVKKYGQEEANKRIYATTDRQKGAVKVEADANGWGTFVVPDDIGGRFSVLTAVGLL
                     PIAASGADIKALMEGANAARKDYTSDKISENEAYQYAAVRNILYRKGYATEILVNYEP
                     SLQYFSEWWKQLAGESEGKDQKGIYPTSANFSTDLHSLGQFIQEGTRIMFETVVRVDK
                     PRKNVLIPTLEEDLDGLGYLQGKDVDFVNKKATDGVLLAHTDGDVPNMYVTLPEQDAF
                     TLGYTIYFFELAIALSGYLNAINPFDQPGVEAYKRNMFALLGKPGFEELSKELNARL"
     gene            56019..57479
                     /locus_tag="VYN69_000204"
     CDS             56019..57479
                     /locus_tag="VYN69_000204"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEV1532561.1"
                     /translation="MSKDIRVRYAPSPTGLLHIGNARTALFNYLYARHHGGTFLIRIE
                     DTDRKRHVEDGERSQLENLRWLGMDWDESPESHENYRQSERLDLYQKYIDQLLAEGKA
                     YKSYVTEEELAAERERQEVAGETPRYINEYLGMSEEEKAAYIAEREAAGIIPTVRLAV
                     NESGIYKWHDMVKGDIEFEGGNIGGDWVIQKKDGYPTYNFAVVIDDHDMQISHVIRGD
                     DHIANTPKQLMVYEALGWEAPEFGHMTLIINSETGKKLSKRDTNTLQFIEDYRKKGYL
                     PEAVFNFIALLGWNPGGEDEIFSREELIKLFDENRLSKSPAAFDQKKLDWMSNDYIKN
                     ADLETIFEMAKPFLEEAGRLTDKAEKLVELYKPQMKSVDEIIPLTDLFFSDFPELTEA
                     EREVMTGETVPTVLEAFKAKLEAMTDDEFVTENIFPQIKAVQKETGIKGKNLFMPIRI
                     AVSGEMHGPELPDTIFLLGREKSIQHIENMLKEISK"
     gene            57493..57927
                     /locus_tag="VYN69_000205"
     CDS             57493..57927
                     /locus_tag="VYN69_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1532562.1"
                     /translation="MDIWEKMYEEAQKLYNPHEVSDFVYANHVVAAVEAGDGQIFTGF
                     CMEGTCGVFHLCAERAALFNMYQFSGQTKVKKVLAFRDKPPYGGSSAMPCGACREFLL
                     ELNAENKDAEFMMDYNIRKTVKVAELIPYWWGEERASKFNER"
BASE COUNT        17675 a        10553 c        12426 g        17320 t
ORIGIN      
        1 aaggtcttta ctaggtctat tttggatgta ttcaatattt tcttgtaaga atggtgataa
       61 atctctagct atttggtttt ttatatcagt gtttagattt ctataaaagt taaatgcttg
      121 ccctaatgta agttcattga tgataaccca gaaaggaaca acgccatgtt gtttatagtg
      181 gtgttttata gaattgtttt gcttatccct attataatca ttgagtattc tagctaatat
      241 actgattaaa ttagtaattt gtgctagcct ttttgtatct ttttgtgttt caaaattatt
      301 aatatctaga taagcgtatg gtgttggata tgcttctgaa aaacaatatg ccagtacgga
      361 tttaaagtgt ttttctgcat ctataattgc ttttaaaaaa gctgatttaa tttctttatc
      421 gtaaaagtgt gttgcagcta tttctctaaa gtctgcacca gttataaatt tatcttttga
      481 tgtttgaaaa aacttactat atccgttgac gacgttgtaa tagttgttag ttagaaggta
      541 tcttttacat tcttctatgt tatctatttc taaacctcta gactggagaa gttttatttg
      601 ttcatctaat gtttgaaatg gtttcatata tgttccttat atacaaaaac accacctagt
      661 agaactaggc ggtgtttcgc gtactctgtt cccttgggaa cagaaactct tctcattgcc
      721 taataatact gtaattttta gtttttgtca atctttttgg tttgtttttt aaaaattttt
      781 gagtatcgaa aaatctcaac gctgggaaag ttcgcttttt ttcgtcctaa aactgtccct
      841 tccgtgttgt ttcgtagtct agtgattctt tgaagaggtc agctagtcca gttacgtctt
      901 ggtttgcgag gagttccata taatgcgccc ttgagtcttt tgagacgatg ataggagcgt
      961 ttagatactt catagctagg tacatgagta aaaggcgccc tgtacggccg ttgccgtcgc
     1021 taaatgggtg aatcccttca atctggatat gggtatctgc taaaatctct aagatttctt
     1081 tttcattctg agcatgattt aagcgataag cggtattatc agcccactgc gtcatcaggt
     1141 agggcgtttc tgctgggcta gccgtttgaa attccacccc gataatagca ttttgaactt
     1201 gcttaaattg tccgcgatca tgttgcagtc tatccactaa taaggcgtga aaatcttgaa
     1261 cgagccccac agtaagggct tgatggtttg ccaatgagtt caagagataa gaaaaggcct
     1321 gtttatggtt ttcaatctca taaaactcac gaatactttt gccgttgcgt ggcaaggtgc
     1381 tttctaagat aatgctgacg gtttcgggta gggaaatagt gttcccttca ataccgctag
     1441 aatggtaggc tatgcgtacc agtatgtcgt ccaggtagtc ttgagcgtag ttcatgattc
     1501 tcctttctat tcttcaattt cctccatttt tgcacaatag accatgaaac gatggttgct
     1561 attggcgaat ataggggatt ttttaattta cgatgagttc tccaataggg ataaaatctt
     1621 tctgttttga agattttgcg attaagtcgt attgatcagc agatttttca tacccaaggg
     1681 aaagagtagt attgtcgtct ggtaattttt tagcaaactc agatatagac atccgaaata
     1741 cggtaattgc attttgctga ttagttgtag cagaatttga attgattgcg tccatagtct
     1801 cttttgcgct atctttagcc gttccagtta gcaaaatcat gattgcgtca tgcggttcgg
     1861 atgaatctga atcgattaca ttattttgaa tttttacgct tattgcgcca gttgattcag
     1921 gatctaattt taatttaatt tcagagatta aatcatcata tttactgttg tctactttgg
     1981 cttttgtatt tgttgaagta gtgttttttt gctccgtttt tggttgctcc gagctatctt
     2041 tggtagctga ttgattgtta gagcaagcta ctagaacagt agcagtaagc aagattgctg
     2101 atgtggttag taattttttc ataagtagcc tcctatatgc tgatgttttc taataaaaga
     2161 tggggaattt ttatagaatt ttctcaaaaa ccattgtggc ttggatacgg tcaccaccac
     2221 ctaatccttt actcccacca ttggcggttg tgatggtatg taggcgataa ccttttgaag
     2281 cttgtttatt gataacatct tctaattctg taaggtttcc tgatccagta ccgaaaaatt
     2341 tttctttcaa agttacctga aggacaacgt agtgtagtcc atttactcct tttcgtcgtt
     2401 tctttcagat agttttttta ctaaattaaa agctattttt ttatcatagt catttaaaga
     2461 aatatagttg attaaaatat ctgcaaaatt tgtattgttc tctttatcag tatttattag
     2521 ttcttcaatt gtaaacatat acgatgggat atgattaagc aatccttttc caaaattgtt
     2581 atactttaca ggattattta tatgttcttc aacgtttttg tacgctgaac taagttcatt
     2641 tattttttta atatctatat cattaagtaa ttttttaaat tcgttacttt ggattatttt
     2701 taaaatattt tgttcatctt cgtgccctaa taagtaagcg acacttactc cgaagtgctc
     2761 agctaaagct gaccatatat tttcatctct aggttttcta atttcatttt cgtagtacat
     2821 tagttgacta tcggatactg ttatatcgta ttcttctttc aaaatgtttt tgagtttatt
     2881 taaagatatt ttttctttct ccctcagttc tttaagttta attttcatgt gataccacct
     2941 aattcaagta ctttttgaat tgattatatc atagtaaaaa aatattatct acaaaaaatt
     3001 ctcaaaaaaa gaacaaaaac gcttgacatt ctcaaaataa gattatataa tcaatttgtt
     3061 cttgaaatga gaataaaaag ggaaaggaga aagaaaaatg cttatcacac aagaacaagc
     3121 gaaagcatta aggagaaaaa aagctgattt gcaactaaaa aattatgaac tagctctaga
     3181 aattggtgta gcgtctagaa cagttcctaa aattttgaag ggagattata aagcgcccaa
     3241 aagaatatat gctagtgtta tggagtggct tgccaaagat tattagaaag gagcgaacca
     3301 atcgtaatac tactctacat ttatagattt ctcatgtggt gttttactac tggggattga
     3361 taaacggatc tagctaaata ttcgctagtg taatttgctt gctacctata gcagtatcaa
     3421 gggtttgtag gggttcatat tctccgattt taccctactt taatgcttta ccttggtact
     3481 gttttaggtg gcaagcactg acaaaagaag aaaggagtaa actaatggaa ctagtctata
     3541 tggacggcaa gaaagagccg tatactacga gcgagattat cgctgaatgt gctgaagtta
     3601 ctcatcacac aatacaagaa cttttgagaa agcataaagc tgattttgaa aactatggaa
     3661 ttatcgcatt tgaaatgcgt aaattagacg gacgagggag accgatgaaa atctatcgct
     3721 tgaacgagca acaggctacc ttgctgatca cttatctaaa gaataccgaa cctgtacggc
     3781 aattcaaaat gaacctagtc aaagcctttt ttgaaatgcg tgatgaactt tctaaacgct
     3841 atcttcaaag agaactggaa aagccaaagc gtaaaagttt aactgaagct attcaaacat
     3901 gggagaaagc acctaagcat gcctatagta ccctgacaaa cctactacta aagggagcga
     3961 cagggaaaaa caagtcccaa ctcatgcaag agcgagaaag taagaacggt attgatggct
     4021 tgaccagtgt agagctgaca aactaccaac gtttggaaga tatggcaata gctatgatta
     4081 acttgaatag gggatattca gaaattaagg aattaatttt taaagcatag gaatatagaa
     4141 aatggaaaat gaatttaaga cagttacaaa tgccaaaggg ttagaaattc ctaagtatcc
     4201 caaggatttt aaaaagctag ttgagaaaga cagacaacta gccgaatatc tttgtatgaa
     4261 ctatgaggac ttagaaagtg aagacctggg cgcatttctt gaaacggtgg agcagggatt
     4321 cagctggatt ctggatctta tcgaaagtaa agatttgctt tataaaccaa agtcaggtag
     4381 taatcatgca aaaagaaaat aaaaaaatca cttgctcaaa ttttagacga ggcgagcaag
     4441 cgacaagatt aaggatatag aaattttttc tatgctccga ttatatcaga aaacagctat
     4501 tttatcaaat acataaagaa aaaccgaaga gcaggcaagc aattagaaaa ggttttgaaa
     4561 tcgagtgctg acacgacgat tctaagcact tgtttagaaa aaatgtgggt gattacccac
     4621 gaaacatcac tacaagcgtc cgccaacttg gggcaaatgc ccagcgtttg gagtggtgaa
     4681 gcataccata tagaaaacag gcaagaaaaa ggacaaggaa aggctaatgg agaaaaatat
     4741 gactctagac ctagataaca tgacacgatc agaatttgat aagctaatga ctaaaatcaa
     4801 ggatagaaat ccgaacctct ttcagttcat cattgacttt ttagatgata aagtaagcac
     4861 cgaagaggtg tatgattttc tgaagatgga gcgaagctat caagtaaatc atatcaagaa
     4921 ttacaaagcg agggcatagc atgaatgaac tagatttaag caatacacag gcgatttttg
     4981 ttaccgtgat attgattggc atattgcttt atttaaacca ccgagaccgc aaaaaaagcg
     5041 cccaaattga gcgagaaaac caacagacga tagaaacacc tagcgaggat ttaaaccctg
     5101 attatgggcg atatatccag cttgcagggg taagagtttc cggagggatg aaatgagtta
     5161 cacagtgaag atataccttg attttgaaaa aaataccaga tgaaccttat tttaccagag
     5221 agatttttct agctatgtgt acggacaatg caacaaagtt agctgtaact atgggtttgc
     5281 tggcaaaaca agagttggat ttagagacta gggctgttat tttaaaaacc cttaatacaa
     5341 tcatagaaaa tcaaaaagct attttgcaag gggtagcgag cggacaaata acctttttga
     5401 ataaaagtaa aggagaggag gaactggatg ggtctatcag agaatgataa acgagtatta
     5461 agattaatca aggtaggggc tgagaactcc ataacagggt tagaaatcag cctgacaacc
     5521 aagctaacag aaagaaccgt gcaagatatt attaagcgtt taataatcaa gcataacatc
     5581 ccaattgtgg aggttagaaa tggattttat agaggttatt tcatacctag aaacaaaggc
     5641 gagttactag atggtactaa ggctttttac aaccaagtac aggaggagag caagcgccta
     5701 gcggtgttga tgaatagcga tttagaaagc tacaaagaaa ccttgaagga ggtgggcgga
     5761 tatgtttagc ctaagccgag aaagcgagaa agacctaacg catggcattc tggaggtagt
     5821 ggaaagatac ctggaagcgc atgagaaagt accgccaaga ttgaccaagt taataaatag
     5881 gattgaactc aaagaagagt taaaaattag tgacaacacg ctgaataagt gggaaagtca
     5941 gggtttaaga cgctatcaac cgccggacga tggttcaaga atgatttact atttagtaac
     6001 tgacatctgg aagtttctgg gggtgtgtga tggcaaagac taaaatatat ttttggttaa
     6061 aagttgataa gaagtttttt gataatcttt ttattaaacg acttaaaaat atgcctggcg
     6121 gctacactat gatagtgatt tatatccgtc ttatgttgga aagtttagaa gatgattgta
     6181 ttttgtacta tgaggggtat tttgataatt tggtacagga gctagcctta aaattggatg
     6241 tgtccgagga tgatataaat atgacagttg catattttac aaaatgcgga ctgattcaga
     6301 tagacgatga tggacatgct acattatcgc aagcaaaagc catggttgag agtgaaacaa
     6361 actgggcaaa atataaacgt gaacagcgta aacaagccca aaatatggca aggttggaga
     6421 gtgtccaaga tagtgggaca gattccaact catgtccaac agagatagag atagataaag
     6481 agtcagataa agataaagag ttatataaag aatatatatt gtcaggtaaa cctgacttta
     6541 ctttcccaaa ttggttaact ccgagaatga tcgaggaaat aactaaagga catcctgaga
     6601 agtatttaat aagaatccct ctagcttatc tgaatcatac agtagggaaa aattataaat
     6661 atttggacaa aaatttgaag ccgataatgg cacgattcaa agaaggctat acacttgaag
     6721 attttaaaca ggtgatagat attaaaacgg cagaatggaa ggatagtcct gaattttcta
     6781 aatatctgag acctgaaaca cttttcggaa ctaagtttga cggttatttg aatcaaaagc
     6841 ctaaaaccat aaaagggaag tcagaagaca acttcccaga cctaccattt taggagttgc
     6901 aaggatgaag gaacaattta aagaatttaa taacagaaaa atatcagata aattttgtaa
     6961 tattcatcag gtaaattact gggaaatttc tatacctgta gtagggagtt cagaaaggaa
     7021 aatacaacca ttttgcccgg agtgtgtgaa gggggagatt aaacaacaag agcaagacct
     7081 attgcagcag ttcgaggaca ggcaagctta ctttaaaact tatgatgtct taatgcgtga
     7141 tagtacgatt cctaacgagt tgaagggggc aacgtttgat aatttctttg tgaagacgac
     7201 agaggaaggt cagatgttag agtttgtaaa ggggcaagct cagaaatacc ttgcaggtat
     7261 gacgggaaac actttaatca gcggtagcac aggaatagga aaaagtcatt tatcgcttgc
     7321 cctggccaaa gaaatcaatg agagtttcag agagaagaac gatcctaaga gtgtcttatt
     7381 tgtcagctta accgagatta tcaagcagat aaaagaaggt tgggcttatg gaagaaatgc
     7441 aaacttaaca gagtatgagg cagttaaaaa gctagttgat gtagattttc taatcatcga
     7501 tgacctaggg gcaaaaaatg ggacggtaac tcctaagagt gactgggaac aggatttctt
     7561 gtttgatatt atcaacaatc gagaaactac gattttcaac acgaacctag atagtagcga
     7621 actgcggacg gtatacaatg ctagaaactc aagtagaatt ttgaaaggtt tagaagggaa
     7681 cgctttcaag gcttttacaa tcaaagataa gcgatatacg attaacacag tgagaggaga
     7741 gaaaggttaa tagatatgga tgaaattaaa ttttcaacag aaaaaggctt tattgtctac
     7801 gaaaaatgtg gtataataga gatagaaaaa attccaagat ttggagaaat aactttagtc
     7861 tactcagatg ggaaatttac tcatctagtc aaaaaagaaa ctaaaaaata agtctattga
     7921 gaacaactca gggacatacc gtaagcattt agtgctagtg gtatgtccct ttttgtttgc
     7981 atagaaaggg ggtgagggag atgtcaggag atacttcttt agggtatgta gtagccaata
     8041 agttttctat ggatccagat aaaagacaga aaatcttttc tcagtgtaaa aaagaaggtg
     8101 atagcttaga acaacggaaa caagaaatac tagagaaata tgctaacaaa caagacaaac
     8161 caaaatctag aaaaaatgat tctaaaggct cggagagtca taaaagaaaa gctaagagca
     8221 aagaatttta gaaaaaatta taaacaaaaa tcagatatta aaagatgaag gagcaaaaaa
     8281 atgacaacta acttagttaa acaaaaagaa aatttagaat cttatatccg aagtacaggt
     8341 tataacacta gagggatgaa tgtagaaaat aatcatgtac tcattgaaaa accaatcctt
     8401 gatagttacg aagatgaaca tcaacgtaaa gaactggttg atctagtaaa tgttattgag
     8461 actcgtaccc gtggcgggaa gtatgaggta actgactttg aatctgattc attacaagaa
     8521 attagtgaaa attttgttga gagaacagaa tcagataaaa agaaaactat cagcgttgat
     8581 tacttagtta aattattcag tggaaaactt gatttttcac aggaacaatt agatgatggc
     8641 caatataatt taacggattt tcttggtaag aagattatta aattaaaacg tagaacacga
     8701 aatagagaga ttgggaaaat tctccaaact gcgaaagtac agaccgctac aagtctggac
     8761 gacttgaaat ctattgtttc tttaatcaat ccagagcgca atgtatctat agttcttagt
     8821 caatcactat ttagtgtctt agaaaaaatg aaagacactt caggaaatta tcttcttaaa
     8881 gttgataaag agacaggaac gagtgaaaca ttctttgtag ataacttttt aattgtagat
     8941 gatacgacat tagggaacga aggcgacaaa aaaggattta tcggagatct agaaaacttt
     9001 gttactttgt ttgatcgaaa gaaagataca cttagttggg tgaatgcgaa tgactatttt
     9061 agaaaacggt tgattttaca tacccgattt gatgtaaaaa aagttgaaga agattgtggt
     9121 tactttattc aatggaacta ggaggaagaa atggatatta atcaagtatt tgaaacactg
     9181 gatgatctag ataataaaaa aagtaagatt aattcagcac gagaacagtt aagcgaaaaa
     9241 aagaaaagcc tgttaggcaa tcaaacagtt tcatttgaga acataaattc ttttttgtca
     9301 aataacttag aatctttaga gcagctggaa aagatggaaa aagctattaa ttctcttcag
     9361 gaaaaatata atagtgattt ttcagaagct aaagcagtca tctttgaata catttttaaa
     9421 gaaactaagc aacggatgga aactaagaag atctataaac aataccgaaa gaaacttaga
     9481 cgaattctgg acgcatatga tgaaattcaa gaactaaaga aggatgtaga agaaattcat
     9541 acaggtgtag tcagggaaat aagtcagaaa cattctctat cgctatatcg aacggaagta
     9601 agtccgctta ctgtcctacc attcttaaac cctgatatta gcgggtggat ggatttttct
     9661 aaggaatatc gggacattaa agagtattta gaaaaatagg gaacaaatta agtaaggcta
     9721 gtgatatatg actgaaacag aagagatatc gctagcccta cttttatgct ttactaagtt
     9781 tcacataaca aagtaagtat aaactaaaat agagtatagg cttggaagcc atatatatca
     9841 gtaagttaca gaatggagtg agtttcacag aatgtaagat atgagaaact gaggggataa
     9901 attaaagaaa tttcccttga acttgtcata ctgaagagtt gtcaaactta aaacaatgat
     9961 acctggtaag tggagtgttg gaaggctttt agcgcttttt gtcagtttga cagaatttac
    10021 aatttgacaa attgcaagat aaaaaaattt ttaaatttaa gtggaggtac ttgcctatgt
    10081 acgagttgag taacagagac ctggacgggg tagatattga gttaggacga tatagaacgc
    10141 ttgctaataa aatttatttg agaagacagg aactaataca taataagaaa catagcgctg
    10201 aagattgtac tggtgggaaa ggcaagacag tatatagtcc tactgaagca accatcatta
    10261 gaattgaaga agaccaaacg ctaagatatt tagaaggctt taaactagtt gtagatacct
    10321 tgatggaaaa cttaattgaa agtgatctag tcatttttaa aatgagatat ttagaagctg
    10381 gtgcgacttg ggaagacgtg gcagagaaac taaataaaac tactcgttat ataaatagcc
    10441 gtagaaaggt aatcgctaaa agatttgttg agttgaaagg atattgactc cccccacctt
    10501 ttaaaaaatc tttctggcca gtagggtacc ggtgaaggga gctttttcca agtcggagac
    10561 ctccagacaa aaagggggta aaaagttgat attttaaaaa atagaaggag tttttagaaa
    10621 atggatttag cagcacaatt agcaaatatt ttagcagaat agtgcgaaga ggttaatgaa
    10681 gaagttgata aaattgcgga gcaagtcgct aaagaaacag ttaaagaatt aagggaaact
    10741 agccctaaaa gagcaggtaa atattcaaaa ggatggcgta agaaaagggt gagaaatgga
    10801 gtttgggtcg tatatagttt taaatacggc tctcttactc atttacttga atttggacat
    10861 attaaacgaa atggggggag gactaaagcg tagcctcatt taagacctgc agaactgaat
    10921 gcgattcaaa aatttacaga aaggattaag aacatttcaa agtaaactat aattgaatag
    10981 tattaagtag actaattaag tgagcaaacc aacacgtcgt tatttaagtc cttagattta
    11041 tttctgaggg ctttttatcc ttgcttctca tattgctatt tgacaaaata aaagtaagaa
    11101 cggttttcgc tctatcctct atgcttttat ctttttcttt tgcaggattc tggttatatg
    11161 taagtttgag aggatatgct ataatattac caggtattaa aaaagcacgt ttgaccgtgc
    11221 tagtttcttg cctgctgaac tcgtcattta ttgccctttt gtggggcttt tttttgtgga
    11281 ctttttgaga atttttaaga acttttaatg atttctaatc aaatgagatt tttcaaaaag
    11341 tcagtaatat caagggttta ggcgctatat tgaggtgtga aatgatgcct aagtcaaatt
    11401 atagagaaat gcgctttatt tcaagtctag gagaaaaatt gcttgctaca aggatattat
    11461 ttacagtgac aattgctaat aatcaatgaa aatcaaagtg caaactagcc acaggttgct
    11521 caaagcacaa ctttgaggtt ggagataaag ctgacgtggt ttgaagagat tttcgaagag
    11581 tataaaatga aaaagggtgg cggggataaa ttatcccttg tcgccaccac ttgtaagtcc
    11641 tgaaacaaag ttcttttgta ggaagaagaa gagaatacag attggaaggg cgatgaggat
    11701 agcacctgct gagaagtagg caatcttcaa gtttttcgca ttgttaacga aggtttggag
    11761 acctacggca acagtaaagt attctttctc acgaagcaag aaactagaga ggatgtagtc
    11821 cccgaaaggt cccatgaagg cccagagagc ttgtacggca accattgggc gaacaagtgg
    11881 tagaacaatt tgccagaagc ggcggaagtg tcctgcaccg tctagttttg cagattcgtc
    11941 taaagacatt ggcactgtat cgaagtagcc tttcatgagc caagcattca tcgggatacc
    12001 accaccaacg tagaggaaga tgaggaacca gttgtggtta agggcgttca acataagcgc
    12061 cataacgaag aaggctgtca aagcggccat agttggcacc atttggatga tcaagaagaa
    12121 gaccaaactt tgtttacgag ccaagaagtt gtaacggctg taagcataac cagcaagtac
    12181 gatgatactt gtttgaacag ccatggtaat taaggcgata atcaaagtgt tgaggtacca
    12241 agtaccgtac aaggtttcag tgaagaggcc tttaaagtta tcaaaattga ggtcgatatt
    12301 agtatctagt ttaaaggctg agacgttacc tgctttaaag gctgacataa tggtaatcaa
    12361 cagtggatag ataattacaa ttgatagacc aatcaggtaa aggtaagtaa ggctttgagt
    12421 cagtctacgt ttgagtttaa ttgagttatt catcttagac gtcctccata tcaaatgcgt
    12481 gtagtttctt gaatgcgatc atagagattg agatgacaat gatagagata atcaaggtaa
    12541 cagctgccgc cattgagtat tgaggagatg tacctgttgt caaacggtag atccatgaga
    12601 tcaagatatc ggttgaacca gctccacctc cgacactacc aggtcctcca ccattgaaga
    12661 ggtacatgat agagaagttg ttaaagttga aggtgtattg gctaatcaaa gtaggtgccg
    12721 caacagccaa aatcattggg aaagtgatgt tgcggaattt ttgccaagcg ttggcaccgt
    12781 caatataagc tgcttcgtaa aggtcgttag gaatagattg caagataccc aaagtcaaaa
    12841 cgtagatgta tgggaatccg agccaacctt gcatcataat caaggcaatc ttagtccaag
    12901 ttgggtctgt tttccaagga ataagagctc catcaaggaa aggaaggaat ttagccaaga
    12961 ttggcaatac ttgagtgttg atagcaccga cactatcgtt aaacatattt gagaatgtca
    13021 agatagtgat gaaggctggg acagcccaag gaagaaggaa aataacacca aagatacgtt
    13081 ttcctttgat aaatggttgg ttagcaatga tagctgtgaa gataccaatt acgatttgta
    13141 aagtagaagc tgccaaagcc caaatgatag tccaagaaag aacagaacca aaggcagaac
    13201 ggaaggtact caagctccaa atgtttgtaa agttggtcaa accaacccag tccaacaact
    13261 tgtttggtgg caagtgttgg aagtcgtagt tggtaaaggc gatcatcaag gttacgataa
    13321 ctgggaagat aatcgcgaag gtcatggcaa cataagatgg aatgatcaag aggtaaggga
    13381 agccattttc ataaatccct ttgatcatgt ctttgagtgt gcgtggaact ggaattccat
    13441 tgttaatgcg ttttgcaatc gtatgtgcat ctttgatatt tgagaaataa aagagtacat
    13501 aaacgattac aaagattaga tggaaggcac cacgaatcag cataaagagg gaattatcac
    13561 gacctggttt gtcaccaaga gtgatgaggt tgctcaattc aggggttgca agtgctagga
    13621 agtaaaggac aaatacgatg gttacaccaa ggaagataaa acctttggct ttttgtttat
    13681 tgtaaatctg tcctaaccca ggaatgatag acagcagggc tgctttacta ggttgttgct
    13741 tttccatatt aactcctttc ataggatact acttttttat aagaaagcta aattatttgt
    13801 gtctctattg ataaattcaa agggggattt gatttccacc ccccttgaac aaatttttta
    13861 ttcaccaaat ttttgtttga ttgtttcttt gatcaatgtt acagcatcgt tagcagctgt
    13921 tttagcatct ttttgaccac ttacagcatc aaagagcata tttttcgctg gatcccaaac
    13981 tgcagacatt tgagagatgt ttggcagtgg ttgagtgttc ttgaactgtt tgataacagc
    14041 tgttgtcaac tcatcgtttt taccttcagc gtatgaacga gcctcagtat tagctgggat
    14101 ttcgttagtc ttatcatata atactttttg ttgttcagtt gcaacaagga agtctacaaa
    14161 tttttgagaa gcttcaaggt tcttaacggc ttgaggaatg acccaagctt taccaccacc
    14221 gaatgcagca tattcttttc catttggaag agttgggata gttgcaactc cgtagtttac
    14281 tttagcatct ttaaaggctt gagctttcca aggtccgtcg atgatagcag ctgttttacc
    14341 ttcttggaat tgagtttgga ttaagtttcc agcaccttct gtatcttgca tacctttagg
    14401 ccatttttcg taccaagatt tagcgtagtt gatacctacg atagaaccgt cgtttgcaag
    14461 accgatgtct ttagcgtctt taccgttttg gccaaagacg taagcaccgt taccggcaag
    14521 aagtccatat gtatagtaga agtttgtcca gtcagctagg aaggcagtag ttttaccatc
    14581 ttcaccagcg aatgcgtatt tgctatcttt agcaaggttt tccaagtcag caaatgtttt
    14641 tggagcgtct tttaccaagt ctttgttgta gtacataaca agtgactcga taacggcagg
    14701 agcaccgtaa actttaccat tagcagctgt tacaagagat ttagttgtgt cgtctgtttt
    14761 agcaccatcg ctcaatttca cttctgaaag ttgtccgtca gaaccaaggc tacctacacg
    14821 gtcgtatgga gccatcataa catcagggac attaccagat tggttgtcaa gagaaagttt
    14881 atcaagacct cctagagcat caccagtttt aagagtgatt tttactccag cttctttttc
    14941 ataagcttta gcaacctctt caatatagct cttatatccc tcgtctacat atacagtgag
    15001 ttctttgact tcagatgaac cagaatcagc aggcttatca gcagttttgc ttccgcaagc
    15061 taccaaaagc aagctagcaa gtgtaacagt tccaagcacc gcagtgctct tcataaattt
    15121 agatgacata gtgtattcct cccaaagaat agcaagtttt attgataagg aaacgcaaac
    15181 gttttccttt atgagcttag tatagcacaa atagaaaacg gttgcaagta ttttttgtaa
    15241 aaattttaaa aaatttttaa gcttgatttg atagcataat tttgcatttt aatagaagaa
    15301 aagtatgaaa tagataagaa tacagtattt ttaataaaaa tatatggaat ctgtctaaag
    15361 agggaaacca ggaggggcta cctccctgtt tctaatccct atctattttc tatagacgtt
    15421 tgtgctttgt tacatataaa ttcaagctcc ctaaggaggt tttgtacttt gtagactgtc
    15481 tcgtgagagt caaactttag gattagatag ttttaagtta tctttgctaa aatgagttag
    15541 tctccttaaa aataatcaaa aaagtttttt aaaaacgctt gcaattatgc ttgaaaagga
    15601 gtatacttat aagtaacgca aacgtttgcg tctgcaaaat acgcaacgtt ccattatttt
    15661 aacacacgag gtgctattat gaaaaaacgt caaagtggtg tgttgatgca catctcttct
    15721 cttccaggag cttacggaat cggatcattt ggtcaaagtg cttacgactt cgttgatttc
    15781 ttggtccgta caaaacaacg ttactggcaa atccttccat taggagcaac tagttacggg
    15841 gattctcctt accaatcttt ctcagccttc gcaggaaaca ctcattttat cgatttagat
    15901 atcttggtgg agcaaggttt gttgggagcg agtgaccttg aaggagttga ctttggtagc
    15961 gatgcgtctg aagttgacta tgctaaaatc tactatgcac gtcgtcctct tttagaaaaa
    16021 gcggtgaaac gtttctttga agtcggagat gttaaagatt ttgagaaatt tgctcaagac
    16081 aaccaatcat ggcttgagct ctttgctgag tatatggcta tcaaagagca ttttgacaat
    16141 cttgcttgga ctgaatggcc agatgcagat gctcgtgctc gtaaagcttc agcacttgaa
    16201 agctatcgtg agcaattggc agacaagttg gtttaccacc gtgtgactca atacttcttc
    16261 ttccaacaat ggttgaaatt gaaagcttac gctaacgaca accacattga aatcgttggg
    16321 gacatgccaa tctacgtagc ggaagattca agtgatatgt gggcaaatcc acatctcttc
    16381 aaaacagatg tcaatggtaa ggctacttgt atcgcaggat gcccaccaga tgagttttct
    16441 gtaactggtc agctttgggg taacccaatc tatgactggg aagcaatgga caaagacggc
    16501 tacaaatggt ggattgaacg cttgcgtgaa agcttcaaaa tctacgatat cgttcgtatc
    16561 gaccacttcc gtggcttcga atcttattgg gaaatccctg ctggttccga tacagcagca
    16621 cctggtgagt gggtgaaagg tccaggctac aagctttttg cagccgttaa ggaagaactt
    16681 ggtgagctaa acatcatcgc agaagacctt ggcttcatga cagatgaagt gatcgaattg
    16741 cgtgaacgta ctggcttccc aggaatgaag attcttcaat ttgccttcaa cccagaagac
    16801 gaaagcattg atagcccaca cttggcacct gctaactcag ttatgtacac aggaacacac
    16861 gataacaata cggttcttgg ttggtaccgt aatgagattg atgatgcgac tcgtgagtac
    16921 atggctcgtt acacgaaccg taaagaatac gaaacagtgg tacacgctat gcttcgtaca
    16981 gtattttcat cagttagctt tatggcaatt gcgactatgc aagatttact agaattggat
    17041 gaggcagctc gtatgaactt cccatctacc cttggtggaa actggtcttg gcgtatgact
    17101 gaagatcaat tgacaccagc tgtcgaggaa ggtttgcttg acttgacaac aatttatcgc
    17161 cgaattaatg aaaatttggt agatttaaag aaataagaca ataatcagga gacaactaaa
    17221 catgttatca ctacaagaat ttgtacaaaa tcgttacaat aaaaccattg cagaatgtag
    17281 caatgaagag ctttaccttg ctcttcttaa ctacagcaag cttgcaagca gccaaaaacc
    17341 agtcaacact ggtaagaaaa aagtttacta catctcagct gagttcttga ttggtaaact
    17401 cttgtcaaac aacttgatta accttggtct ttacgacgat gttaaaaaag aacttgcagc
    17461 tgcaggtaaa gacttgatcg aagttgaaga agttgaattg gaaccatctc ttggtaatgg
    17521 tggtttggga cgtttggctg cctgctttat cgactcaatt gctactcttg gtttgaatgg
    17581 tgacggtgtt ggtcttaact accactttgg tcttttccaa caagttctta aaaacaacca
    17641 acaagaaaca attccaaatg catggttgac agagcaaaac tggttggttc gctcaagccg
    17701 tagctaccaa gtaccatttg cagactttac tttgacatca actctttacg atattgatgt
    17761 tactggttat gaaacagcga ctaaaaaccg cttgcgtttg tttgacttgg attcagttga
    17821 ttcttctatt attaaagatg gtatcaactt tgacaagaca gatatcgctc gcaacttgac
    17881 tctcttcctt tacccagatg atagtgaccg tcaaggtgaa ttgctccgta tcttccaaca
    17941 atacttcatg gtttcaaacg gtgcgcaatt gatcatcgac gaagcaatcg aaaaaggaag
    18001 caacttgcat gaccttgctg actacgcagt tgtccaaatc aacgatactc acccatcaat
    18061 ggtgattcct gaattgattc gtcttttgac tgcacgtggt atcgagcttg acgaagcaat
    18121 ctcaattgtt cgtagcatga ctgcctacac taaccacaca atccttgctg aggcgcttga
    18181 aaaatggcct cttgaattct tgcaagaagt ggttcctcac ttggtaccaa tcatcgaaga
    18241 attggaccgt cgtgtgaagg cagagtacaa agatccagct gttcaaatca tcgatgagag
    18301 cggacgtgtt cacatggctc acatggatat ccactacgga tacagtgtta acggggttgc
    18361 agcactccat actgaaatct tgaaaaattc tgagttgaaa gccttctacg acctttaccc
    18421 agaaaagttc aacaacaaaa caaacggtat cactttccgt cgttggctta tgcatgctaa
    18481 cccaagattg tctcactact tagatgagat tcttggagat ggttggcacc atgaagcaga
    18541 tgagcttgaa aaacttttgt cttatgaaga caaagcagct gtcaaagaaa aattggaaag
    18601 catcaaggct cacaacaaac gtaaattggc tcgtcacttg aaagaacacc aaggtgtgga
    18661 aatcaatcca aattctatct ttgatatcca aatcaaacgt cttcacgagt acaaacgcca
    18721 acaaatgaac gctttgtacg tgatccacaa ataccttgac atcaaagctg gtaacatccc
    18781 tgctcgtcca atcacaatct tctttggtgg taaagcagct ccagcctaca caatcgctca
    18841 agacattatc catttaatcc tttgcatgtc agaagttatt gctaacgatc cagcagtagc
    18901 tccacacttg caagtagtta tggttgaaaa ctacaacgtt actgcagcaa gtttccttat
    18961 cccagcatgt gatatctcag aacaaatctc acttgcttct aaagaagctt caggtactgg
    19021 taacatgaaa ttcatgttga acggagcttt gactcttggt actatggacg gagctaacgt
    19081 ggaaatcgct gagttggttg gtgacgaaaa catctacatc tttggtgaag attcagaaac
    19141 tgttatcgac ctttacgcaa aagcagcata taaatcaagc gaattctacg ctcgtgaagc
    19201 tatcaaacca ttggttgact tcattgttag cgatgcagtt cttgcagctg gaaacaaaga
    19261 gcgcttggaa cgtctttaca atgaattgat caacaaagac tggttcatga ctcttcttga
    19321 tttggaagac tacatcaaag tcaaagagca aatgcttgct gactacgaag accgtgacgc
    19381 atggttggat aaagtcatcg ttaacatttc taaagcagga ttcttctcat ctgaccgtac
    19441 aatcgctcag tataacgaag acatctggca cttgaactaa tactcttcga aaatctcttc
    19501 aaaccacgtc agctttatct gcaacctcaa agcggtgctt tgagcaacct gcggctagct
    19561 tcctagtttg ctctttgatt ttcattgagt ataagataca aatttatact aatacatctt
    19621 gtaaaaaagc gagtttcaat tgaaattcgc ttttttaaat gctgtggatt tgggtcaatc
    19681 ttgtctaaaa attgggaaat cctagataca gtgaaggctt taaatgctgg tttttactgt
    19741 cttcagcctt atattttttc gtagttggtt acctcacatc tattgtattc gcttacataa
    19801 agtattataa tataattgta ggaaagaagg tgtttttatg atatacacac ttaaattggt
    19861 gttgtttatt acctttcttg taataagctt gttacctgat aagatttttg gaaaaaataa
    19921 aaaaatttgg aaaatagttt ttgtaatatt gacggcagtg gcagcattgt catttatgta
    19981 ctaagttatt ttaagaatgt agggaaataa accctacatt tttttagttt tttctgtttt
    20041 ctaaattcta tttatccaag agcttcaaca tttcttgctt cttcgcttca agttctgcac
    20101 gcttttcttc gatttcggca tgttttttct cgagttcaga acaacttgca ccattgctaa
    20161 attcttttcg ccatcaggag atagggtgag tctacatgtc tattactcac ccaaagcagt
    20221 cctacaaagc aggaattttc tgttactttt ttggaaatag taacgtttat acagctttga
    20281 cacttcgtat caaagcgcca aacacactcc gagggattta cagaaagcag aaaaggaatg
    20341 atctggtata agatcattcc ttttctctct ttttctttaa gtaattatat acaatgtacg
    20401 acgaagtcgt cattgcaatg ctgatccacc acctaaaggg aactttaaac aacattgata
    20461 agataaagaa tataaacaac gaaaatacgt tatacccaat taattttatt gtatatctca
    20521 tgattaaaag ttaatccttc cgttgttagg aatggcatca tttttatccc ataattgtgt
    20581 taaataagtc cccggtgata ataaattcat agcggattct aaagcaacat catttacaaa
    20641 ccaactacct agatatctag aaattgctga acgaatagca ctttttgctg catgttttcc
    20701 ttttacttta attagatttg caaggcctgc agtagttcct cctaatgcta aagctattgc
    20761 agtatctaat agagcaccca tttgattaac tgtaatacct tgccaagctg ctctaaatgg
    20821 agagtatgta ggtgggattg tataatcgcc ttgtaattgt cggttaatta cttctttgat
    20881 ccattgttgt gagacgtctg gatgaaaaga ttggatttcg tttgcaagtg tattgatttg
    20941 ttcttctgtt agagaagtga caggttgaag ttccatattt gtttcaattt gtgatacttg
    21001 ttcagaagcg tatacagctg aaacacttgg aatcgctgat acaattaaca caattgacgt
    21061 caaaaaaacc gaaataaatt tcattaattt gttcatgagc ttttctcctt tttatttgta
    21121 tctgcttaca ttttatcata tactgttatt atagtcaaaa aaatatgcta ttatgttaaa
    21181 aaaatatttt tcaaaatata aatggacgga tttattttgg attttatttg ttattttgac
    21241 ctgcctctat attggtaacc atgatttgtt tactctcaat catcaagaat tctcttttcg
    21301 tggtagcgtt tggggtctgg tactggcctt atatcactta ttgttcattg ataagtttgt
    21361 aatttcgaat cgaaaataaa gattagaact atgattgact ggctactttt aatattgatt
    21421 ttggaggaag attttgtctc tattatttat tattttaaat ttatttattt tgtataagat
    21481 ctattctttg aggcgggaaa aatcgaaata cttgatttat acggcctata tcatatttgg
    21541 ggtaaatgta gtatatggta ttcaatggtt attaagagaa ctgatttcaa ctatttcccc
    21601 ttaatgtgta tggaaactat aacaaaacgc ataagattaa ggtttgtgta cctgttctta
    21661 tgcgttttta taatattgaa gattttcggt aagttttata cctttgggaa gtctagttta
    21721 gaatgctttc ccaaccaaaa tctctgcttc gatggtaatc tctgtcagtt ggctccagtc
    21781 aatcttgctc tggtcaacgt gaaagaggag aggagtcata ctgagtaggg cttggagttg
    21841 ttctgctgtg atagtcttag tcagagaggc agtttgacta gataggatgg taaagtgttc
    21901 ctggaaatgc tccttgatat cttggttaga atactccttg tttgtcagct ggtcctgtac
    21961 tctttgacgg atttctttga ggtgattttc agttgggata acctttatca agataccgtc
    22021 tttggataaa acgcgacgaa attctccata gttggcaggt gagaagatat caagcagaat
    22081 atccatgtta gcgtctttta taggaagtcg tgccaagtcg ccaacgaacc aattgactgc
    22141 ccagttgggt tcacttttag cagcgatttg gactgaatct ttggagatgt caaaggcata
    22201 gaaagttttt tcagagtgac tttcttgtag tttgcgagaa tagaatcctt caccacaacc
    22261 gatatccaaa attgttgtgg tagtttttga gcttgcaagc aagtcagata cagcatctaa
    22321 gatagcttgg taaaagccgg cttctaggat ttgttgacgg ttttgaaaat tttccttgtc
    22381 atagttagca gattgcttga tttgaggtgc tagattgaca tagccaaatt tcgccaagtc
    22441 aaaagaatga cggttgcagc acttgaaatt agtctctaac agagtcagat tttcttgaca
    22501 gataggacag gcaaaggtag tcgcagaagc aaaacgctga agtttgggct tgagatttgt
    22561 attcatagtt taagtgtatc aaaagaggca aatgaaagca actttaggaa taagtagatg
    22621 ggagattcag tagaaataaa actaattctc caatttatag aatgaaattg cttttatatc
    22681 taaattgcta tataataatg ataaggagga aataagtatg ttaaaagaag tattaaccgt
    22741 tgcaaaagtt gcgaaaaaat cttcattatt tttgggtggt gtcgcatttg gtacccttgg
    22801 tttgaaaatc ttagcaagta aggaagctaa aaaaggttat tctaaagctt tggctaaggc
    22861 ttacaagttg aaagacgagc tagatgcatc tgtttctgtt gtgaagcaac atggagacga
    22921 tgtcttgcaa gatgccaaat atttgtacga gcaagagaaa aaagaagagc aattagatag
    22981 ccttataggt gaataatatg tcttttaaag tgctacatag aggataccaa catatccgac
    23041 tatcatcttc tttttcactt accttggata ttcaagacta tcttcgttcc ttggcgagag
    23101 atgaaaaggg gattgagtct atccagtttt acatggatca acagcacttt actctacgca
    23161 taaaagaagg cttttctgta ttagataatg cagaagcctt tttaaaaaga attgataaag
    23221 ggaaagtttc tgagttgatg actcttccca ttcgtagaga agagagtgct tattctattg
    23281 tttcaggtgc agcggttaag cgtgtacttt ttcgtagttt tgtgccgtat cctattcgct
    23341 atatatggac ttgttatcag gctttgggtt atattagaga agcctatcaa acactagcgc
    23401 gtaaggaact aacgatggaa gtcttggact gttcggctat tttattgtcc ttgtttatga
    23461 accaatccaa gacagctagc aatatcatgt ttatgcttga tttggggaat catttagatc
    23521 agtggtcctt gaaaaaaact gcaacagatt tagaacagag tcttcttgca aaagagagcg
    23581 atgtattcct agtacagggc gatacggttg ttagtatcaa gagttccgat gttcaaatag
    23641 gagatgtctt gatcttatct caaggaaatg aaattctgtt tgatggacaa gtagtttcag
    23701 gtttaggtat ggtcaacgaa agttccttga caggagagag ttttccagtt gaaaaaagag
    23761 agtctgattt ggtttgtgca aatacagtat tagaaactgg agagttacgc attcgtgtaa
    23821 cagataatca gatgaacagc cgtattttac agctgattga gttgatgaag aaatctgaag
    23881 aaaacaagaa aacgaaacaa cgctatttca tcaagatggc ggataaagtc gtcaaatata
    23941 atttcttggg ggctgggctg acttacctat tgacaggttc tttttctaag gctatttctt
    24001 tcctattgat cgatttctcc tgcgctttga aaatctctac tcctgtagct tatttgacag
    24061 ttatcaagga ggggttgaac cgtgaaatgg tgattaagga tggagatgtt ctggagaaat
    24121 atctggaagt tgatactttc ttgtttgata agacaggaac aatcacaact agttatccta
    24181 tagttgaaaa ggtgttacct tttggagact atagtgagga agatattctc agaatcagtg
    24241 cctgtcttga ggaacacatt tatcatccta ttgctaatgc catcgtcaag caagctgaga
    24301 tagagggaat tgaacatgag gaaatgcatg ggaaactcca atatatcgca agcaagggga
    24361 tcaaatctca tatagatggc caaccagttc ttattgggaa ttatgtcttg atgcaggatg
    24421 agcagattca tatcagttca gaacaaaatg ctttaattga agagtacaag agtcactaca
    24481 atctcttatt cttggcttat caaaatgaat tgattggaat gttctgcatt catactcctt
    24541 tgagaaaaga agcaaaaaca gccttggata aacttaaggc acaggggaaa aaattgattc
    24601 tggcaacagg agacaccttg attaggacag aggaattagt caaagatttg ccctttgatc
    24661 aggtctatac agacttgaaa cctgatggga aatttgagtt agtagagaaa ctgcagaaag
    24721 caggtcacac tatcttgatg gttggagatg gattgaatga ctcagcggct ttaactctat
    24781 cagatatcgg tgtggtgatg aatgagagtg cagatatttc taagcagatg agtgatatct
    24841 tattgttaga taatcgtttg gatttcttcc aagagttgga ttcgttatca tcatctttgc
    24901 aaacactcat caagaaaaat attcaagata ccgttgtcgt aaatagtagt ttgattggct
    24961 ttggcttatt taattggttc agtccttcaa atctctctat cttacataat ctaacaacct
    25021 tgcgcattgt cttgcgtagc ttgtctatta aaaatagata gatgagagtt attaacagca
    25081 aaaaggagtt acctttctcg aggttaactc ctttgtttat agataaatgt tgaatagttt
    25141 agtaagtcaa tacaaagtat tttttcttcc cacgacggat aacagtcagt tcattttcta
    25201 acttatcagc gtcactcaag acatagtcaa ggtcttggat gcggtcgccg tttacgtaga
    25261 tggctccgtt ttggacgtct tcacgggctt ggcgttttga gttaactata ccagatgaga
    25321 cgagcagttc cacgatattg ttatgttcgt ctgcctgtac ttggtagttt ggcacaccac
    25381 gaagtccttg tttgagctct ttgacagaaa ggtttttgat gtttcctgca aagagttgct
    25441 cagtgatgtt aagtgcttct ttgtaggctt cttctccgtg aacaagtgta acaacttcac
    25501 gagccaagac tttttgagcc aagcgttcgt gtggcgctgc ttcaaattgt ttacgaatat
    25561 cttcaatctc atcaagtgac aagaaagtaa agattttcaa gaagcgaaca gcgtcagcgt
    25621 ccatcacgtt catccagaat tggtacattt cgtatggaga agtcttttcg ggattgagcc
    25681 agacggcatt tccttctgat ttaccaaatt tcttaccagt tgcatctgtg attagtggaa
    25741 cagtgataac gtgaccagtc ttgtccgcct tacgacgaag caattcggta ccagctgtca
    25801 tatttcccca ctggtcagaa ccaccgattt gaagagtgac attatggtct tggttaagga
    25861 cgaagaagtc gtacccttgc atgatttggt aagcgaactc agtgtaagaa attcctgttt
    25921 cgatccgttt tttaacagat tccttactca tcatgtagtt gaccgtgaag tattttccaa
    25981 tatcacggag gaagtcaatg aagctgatgc tgccaaacca gtcgtagttg ttgaccatga
    26041 cagccttgtt ttcgccattt tcaaagtcaa gaaaacgaga aagttgtcct tggatagact
    26101 tgacccagcc atctactgtg tcttttgttt ggagactacg ttcagcatct ttgaaggacg
    26161 gatctccgat gagacctgta gcaccgccaa cgagcgcata aggtttgtga cctgctagtt
    26221 gcaagcgacg acttgtcaag attgcgacaa ggtggcctag gtgaaggctg tcagcagttg
    26281 gatcgtagcc agtataataa gaaacttgac cttcttctag ggctttacgc aaagcttctt
    26341 catcagtcgt ttgaaatatc aaaccacgct cttttagctc atcaaaaatg tgcatgtgtc
    26401 ttttctcctt tttaaaatta ttgtttctac ctattgtatc acaaacttgg gcaatagcct
    26461 agcagaatag ggaagaaagt ggctatttta ttgaaaattt cccttttgtg gtataataga
    26521 tagagtgagg aaatccatgc aaaatcaatt aaatgaatta aaacgaaaaa tgctggaatt
    26581 tttccagcaa aaacaaaaaa ataaaaaatc atctagacct ggcaagaaag gttcaagtac
    26641 caaaaaatct aaaaccttag ataagtcagc cattttccca gctattttac tgagtataaa
    26701 agccttattt aacttactct ttgtactcgg ttttctagga ggaatgttgg gagctgggat
    26761 tgctttgggg tacggagtgg ccttatttga caaggttcgg gtgcctcaga cagaagaatt
    26821 ggtgaatcag gtcaaggaca tctcttctat ttcagagatt acctattcgg acgggacggt
    26881 gattgcttcc atagagagtg atttgttgcg cacttctatc tcatctgagc aaatttcgga
    26941 aaatctgaag aaggctatca ttgcgacaga agatgaacac tttaaagaac ataagggtgt
    27001 agtacccaag gcggtgattc gtgcgacctt ggggaaattt gtaggtttgg gttcctctag
    27061 tgggggttca accttgaccc agcaactaat taaacagcag gtggttgggg atgcgccgac
    27121 cttggctcgt aaggcggcag agattgtgga tgctcttgcc ttggaacgcg ccatgaataa
    27181 agatgagatt ttaacgacct atctcaatgt ggctcccttt ggccgaaata ataagggaca
    27241 gaatattgca ggggctcggc aagcagctga gggaattttc ggtgtagatg ccagtcagtt
    27301 gactgttcct caagcagcat ttttagcagg acttccacag agtcccatta cttactctcc
    27361 ttatgaaaat actggggagt tgaagagtga tgaagaccta gaaattggct taagacgggc
    27421 taaggcagtt ctttacagta tgtatcgtac aggtgcatta agcaaagacg agtattctca
    27481 gtacaaggat tatgacctta aacaggactt tttaccatcg ggcacggtta caggaatttc
    27541 acgagactat ttatacttta caactttggc agaagctcaa gaacgtatgt atgactatct
    27601 agctcagaga gacaatgtct ccgctaagga gttgaaaaat gaggcaactc agaagtttta
    27661 tcgagatttg gcagccaagg aaattgaaaa tggtggttat aagattacta ctaccataga
    27721 tcagaaaatt cattctgcca tgcaaagtgc ggttgctgat tatggctatc ttttagacga
    27781 tggaacaggt cgtgtagaag tagggaatgt cttgatggac aaccaaacag gtgctattct
    27841 aggctttgta ggtggtcgta attatcaaga aaatcaaaat aatcatgcct ttgataccaa
    27901 acgttcgcca gcttctacta ccaagccctt gctggcctac ggtattgcta ttgaccaggg
    27961 cttgatggga agtgaaacga ttctatctaa ctatccaaca aactttgcta atggcaatcc
    28021 gattatgtat gctaatagca agggaacagg aatgatgacc ttgggagaag ctctgaacta
    28081 ttcatggaat atccctgctt actggaccta tcgtatgctc cgtgaaaagg gtgttgatgt
    28141 caagggttat atggaaaaga tgggttacga gattcctgag tacggtattg agagcttgcc
    28201 aatgggtggt ggtattgaag tcacagttgc ccagcatacc aatggctatc agaccttagc
    28261 taataatgga gtttatcatc agaagcatgt gatttcaaag attgaagcag cagatggtag
    28321 agtggtgtat gagtatcagg ataaaccggt tcaagtctat tcaaaagcta ctgcgacgat
    28381 tatgcaggga ttgctacgag aagttctatc ctctcgtgtg acaacaacct tcaagtctaa
    28441 cctgacttct ttaaatccta ctctggctaa tgcagattgg attgggaaga ctggtacaac
    28501 caaccaagac gaaaatatgt ggctcatgct ttcgacacct agattaaccc taggtggctg
    28561 gattgggcat gatgataatc attcattgtc acgtagagca ggttattcta ataactctaa
    28621 ttacatggct catctggtaa atgcgattca gcaagcttcc ccaagcattt gggggaacga
    28681 gcgctttgct ttagatccta gtgtagtgaa atcggaagtc ttgaaatcaa caggtcaaaa
    28741 accagggaag gtttctgttg aaggaaaaga agtagaggtc acaggttcga ctgttaccag
    28801 ctattgggct aataagtcag gagcgccagc gacaagttat cgctttgcta ttggcggaag
    28861 tgatgcggat tatcagaatg cttggtctag tattgtgggg agtctaccaa ctccatccag
    28921 ctccagcagt tcaagtagta gttctagcga tagcagtaac tcaagtacta cacgaccttc
    28981 ttcttcaagg gcgagacgat aattctctaa atgaagtggc caatcaatgg attgccactt
    29041 ttttcttttc gtgttacaat aagggtatga atcagtatca gaaaaagatt gttaacggaa
    29101 aaatttattc gctcctatcc ggcttaatat ggggaatctg tggaatttta ggagagtact
    29161 tctttactca ttatcaggtg tcttcgggct ggattacctc tatgcgtttg acactggcag
    29221 ggagtcttgt actcatttgg tctgcaatac aattaaaatc gcaagtgcta gatatttggc
    29281 gagacaagaa aaattacctg ccctttttag cctatgctat tttggggatt ttttcagttc
    29341 agtatttttt ctatctctgt gtagaatact caaatgctac gacagcaact attttacagt
    29401 ttattagccc tgtctttatc ctcttttaca atcgcttggt ttatcaaaaa cgagcgtcaa
    29461 aaagcgctgt tttctatgtt ttggttgcca tgctgggtgt ttgcttgatg gcgacaaagg
    29521 gagatctctc tcagttatcc atgacgccgc tagctcttat aacaggtttg ctgagtgcca
    29581 tgggtgttat gtttaatgtt atcttgcccc aaccttttgc taagcgttat ggttttgttc
    29641 ctacggttgg gtgggggatg attttggcag gtttgtttag caatgtcctc tcgccgattt
    29701 atcagctttc ctttactctt gatatttgga gtatcttgat ttgcctcatt atcgctttct
    29761 ttggaacggc ttttgctttt ttcatttcca tgaaggctgt gtccttggtt tctcctttgg
    29821 tggtttccgt tatcagtgcc agtgaacctc tctcttctgc tctcttgagt gttttgttct
    29881 taggattagt agtggattgg tccctccttc tagctatagc cttgattatt ttacccatga
    29941 tatttttgtc tatagaagaa gcgaaagaaa gtagataaaa agtcttttct taattctaaa
    30001 agtgtgctat actagaatag tcaataaaac acggggagat agttgtgaag agtgttttta
    30061 ggttgtatcg gtaggggctt gcttttaccg acagcacgtt ttatctcaca tccctaaaaa
    30121 atcataccta aaaacagaca aaaaggtttc aaatttgagg ccttttttgg tagaataggt
    30181 atcattataa cgaaccagga ggcacctatg actgctacaa aaatgaacgc tcaagaaatt
    30241 atccaattta tcgccaatgc tgaaaagaaa accagtgtca aagtaacctt tgaggggcaa
    30301 ctcgcaactg ctgtgcctag ctctgttgtc aaactaggga atgtcctatt tggagactgg
    30361 aaggatgtgg ctccgcttct cgatggcctg gtagaaaatc aagactatgt tgtcgagcaa
    30421 gatgctcgta attctgcagt tcctttgcta gataagcgtg ctatcaacgc tcgtatcgag
    30481 ccaggtgcta ttatccgtga ccaggtggaa attggtgaca atgctgttat catgatggga
    30541 gctgttatca atatcggtgc tgaaatcggt gctggaacca tgattgacat gggtgccatc
    30601 cttggtggcc gtgccatcgt tggaaaaaat agccacgttg gtgcaggtgc agttttggca
    30661 ggtgtgattg agccagctag tgctgagcca gtccgtgtcg gagacaatgt tcttatcggt
    30721 gctaatgcag tggttatcga aggagtccaa atcggtagtg gttcagttgt cgcagcagga
    30781 gctattgtta cccaagatgt cccagaaaac gtggtagtag caggtgttcc agctcgtatt
    30841 atcaaagaaa ttgatgccca aactcaacaa aaaacagcgc tagaggatgc gcttcgtacc
    30901 ttgtaattgt aaaagtaaaa aagaggcgga accctttttc cagcctcttt ctgctatatc
    30961 ggaggacaga tagatgttag atttgattca gactagacga gatttacacc agattccaga
    31021 gattggcttg gaggagttca agactcaggc ttatttgctg gatgtgattg agaaattgac
    31081 tacgggcaag gattttgttc aaattcgtac ttggcggaca gggattttgg tctacctgca
    31141 gggaagtcag ccggagcgaa ccattggttg gcggacagat attgatggcc tgcctatcgt
    31201 cgaacaaaca ggtctacttt ttgcttctca acaccaaggt cgcatgcatg cctgtggtca
    31261 tgattttcat atgaccattg ccttaggctg tcttgagcgc gctcttgagg agcaacccaa
    31321 gaataatctg ctctttctat ttcagcctgc tgaagaaaat gaagctggtg gtatgctcat
    31381 gtatgaggac ggtgcttttg gagattggtt gccagatcaa ttctatggtc tccatgttcg
    31441 tccagatttg aaagtcggcc agattgcgac taatactcat acactctttg caggaacttg
    31501 cgaggtgaag atccgtttca aaggcaaagg tggccacgca gcttttcctc atgaagccaa
    31561 tgacgccttg gtggtggcta gttactttgt gacccaagtg cagtcagttg ttagccgcaa
    31621 tgtcaaccca atcgagggag cagtggtgac cttcggcgtt tttcaagctg gaaccaccaa
    31681 caatgtcatc acagacacag cttttttaca tggaactatt cgtgccttga cacaggacat
    31741 gagtctcttg gtgcaaaaga gggtaaaaac agttgcagaa ggggttgcag ctgcctttga
    31801 tatggaagtc gaagtggaac tcaaacaagg tggataccta cctgttgaga acaatccagc
    31861 cttggcgcgt gaactgatgg acttctttga cgaaaaagac ggaatcgagt tgattgatat
    31921 cgaacctgct atgacaggcg aggactttgg ttatctcctt tcaaaggttg atggcgtgat
    31981 gttttggcta ggtatcgata gtccctacgc tcttcatcac cctcaaatga gtcctaagga
    32041 agaagtctta gctattgggg tggctgcggt gtctagtttc ctgaaaaaga aggcagcaga
    32101 atagagggct tgtatatgaa atcggaatta cgcaagcaag tcttgcatga aatgaaggct
    32161 ttatctcaag aacaaaaaca ggctatagac caagctttaa ccgagcgaat tttacaacac
    32221 cccttttatc aagaagccaa ggtcatcgca acctacctct ctttttctca tgagtttcaa
    32281 acgcgggaac tgattgagca ggcgctgaag gacggcaaga aggttttaat acccaaaact
    32341 tatcccaagg ggcgcatgga gtttgtggtc tatgatccgc aacagttggt aaaaacttcc
    32401 tttgccttac tggagccaca gggagatttg gaagtggtgg atgtatctca gattgatttg
    32461 attcatgttc ctggcctggc ttttacgacg gatggatatc ggattggata tggtggaggt
    32521 tattatgacc gctatctgga acatttttct ggtcgtactt tgagtacggt ttatccttgt
    32581 caaattcagg actttatacc tgaaaaccat gatattcctg ttcaggaggt attaattgat
    32641 gaaggaaatc tttgatagac gttaccctgt gacgagtttc ttcctcttag tgacggcctt
    32701 ggtattttta ctaatgttgg tcactgcagg cggaaacttt gacagggcag atacattatt
    32761 tcgatttgga gccatgtatg ggccagctat tcgcctcttt cccgagcaga tttggcgtct
    32821 cttgtctgcc atttttgttc atattgggtg ggaacatttc attgttaata tgctttcact
    32881 ttattatctt ggaaggcagg tagaggagat tttcggttct aagcagtttt tctttctcta
    32941 tcttttatca ggaatgatgg gcaatctctt tgtttttgta tttagtccta aatccttagc
    33001 agcaggcgcc tctacctctc tttatgggct atttgccgcg attatcattc ttcgctatgc
    33061 aacgcgtaat ccttatattc agcagttggg ccaatcctat ctgacacttt ttgtggttaa
    33121 cattattgga agtgttctga ttccaggaat cagcctagca ggccatatcg gtggtgcagt
    33181 tggtggcgca tttctagcag ttatctttcc agttagagga gaaaaacgga tgtataacac
    33241 cagccagaga ttaggagcgg tagtcttgtt cgtaggactc gccattttgc ttttctacaa
    33301 gggaatggga atgtgacgaa gttgaaagaa aatttgtgta atgaaaaaag ataaggcatt
    33361 ttttgaacct tatcttttta ttttattcct tctcagtcaa ttcttttcca agttggatga
    33421 ggtaattctt caaatcatct ttgacttgtg ggtgtttgag ggcgtagtcg atggatgttt
    33481 tcatgaagcc aaacttgtct ccgacatcgt aacgagcccc tttgaactca cgagcaaata
    33541 cacgttgtgt tttattgagg gtgtcgattg catctgtcag ctgaatttca tttcctgcac
    33601 ctggagcttg cttttcgaga atctcaaaaa tttcaggcgt gaggaggtag cgtccgataa
    33661 tagcaaggtc gctaggagcg tcctctggag ctggtttttc aacaaaggtt tcaacactgt
    33721 aaagaccatc ttttccttcg ccttgcggag caataacccc gtaagcagat acttcgtcat
    33781 gagggactgg catgacagcg atagtagacg cgtgggtacg ctcgtagtca tccatgagtt
    33841 gtttggtaag tggaacagcc ttttcgtctg tgatatccat caagtcatca ccaagcataa
    33901 cgacaaaagg ttcatttccg acgaaagcct tggcttgcaa aacagcatct ccgagaccgc
    33961 gtggatgagt ttggcggata aaatgcagac gcatgccagt tgtttcctca actagcttca
    34021 aaagatctgt tttccctttt tctttgaggt tatattccaa ttcgaagttt gaatcgaagt
    34081 ggtcctcaat agaacgtttt gatttaccag tgacaactag aatatcttca atacctgatt
    34141 tgagagcttc ttccacgata aactggatag ttggtttgtc tacgattggc aacatttctt
    34201 tggcaagggc cttggttgct ggtaaaaatc gagttcctag tccagcagca gggataactg
    34261 cctttctaac ttttgatgtc ataaaaatcc tttctataga gggttaagac cactcatttt
    34321 ctgctttaaa ttcattgttc atgatgtcat aaatggcatc tttgatattg gttccgtgat
    34381 aaataacttg gtaaatagcc tgtgtaatgg gcatatagac tccaagttct tgggctagtt
    34441 cataggctgc tcgagtcgtt gaaattcctt cgattaccat gcccatatta gcttctatat
    34501 cagctaggga ttctcctcgt ccgagagcat ctccagctct ccagttacga gagtggatgg
    34561 aagttcccgt tacgatcaaa tctcccacac cagataagcc gctataggtc aatggactgg
    34621 ccccgagtgc tacccctagg cgggtgattt ctgctaaacc tcgagcgatg atggctgcct
    34681 tagcattatc accaaatcca agaccatgta aagctccagc accgacagca ataatatttt
    34741 taagagcacc agcagtttca accccgataa catccgtatt ggtataaagt cggaagtagt
    34801 gattactaaa tagcttctga acgtattgag ctgtttgtaa atctttagaa gcagcagtta
    34861 ttaaagttag gtcacgcaca atggtctctt ctgcatgact aggccctgaa acaacgacga
    34921 tatcactacg gagatgttca ggaatttctt cttcaagaat ggttgataat cgtttatggc
    34981 tatcaggttc taatcccttt gatgcgtgca tgatgataac cttatggtcc aaggtttgtg
    35041 caacttgctg ggcaacaagt cgtgtcactt ttgttgggac aacaaacaaa atcgcatcca
    35101 catctttcaa tgtttctgct aagtcggtgt aggcaatgat attttcgtct agaacgacat
    35161 ctttaaagta gtgcttatta gtatggtgtg tattaatttc attgatttgc tcgggaagat
    35221 ttccccaaat acgtacctcg tgtccattgt catttaagac ttgtgaaagg gcggttcccc
    35281 aagaaccagg ccccaagacg gcgacggttt gtttttccat gatttcctcc ttgatagagt
    35341 tctttctctt attctatcat attctagggg attttgcact tgcattactg gggtatggtg
    35401 gaaaaataca caaaaaccga gacgaaggga taaactctta gtctttagtt aaagtgagaa
    35461 gaaatctgat agatagtttg attcagtata tgcttataat agaggtaaac aactcaggag
    35521 gttcttgtag gttgcgagag tttggcgaaa aaattaaaag attacgtttg gctaaaaaaa
    35581 tcagtcgttc agaattttgt ggtgatgagt ctgaattaag tatccgtcaa ttaattagaa
    35641 ttgaaaatgg agaatccaga ccaacactaa caaagttaaa atatatcgct gaacgtttgg
    35701 gggttgaaga ttacaagttg atgccaagtt atatagagtt ggataaggaa tacctagaat
    35761 tgaagtattt cttgatgaga actcctacat acgaagatga aactatcgcc caaaagaaag
    35821 agagtgtttt gataagattt ttgaagagta ttatgatagg ctacctgagg aagaaagatt
    35881 tatcatccca aattattcat atctggcact aacgaactac acagttcaaa aattaccaga
    35941 aaagctagtt gaaatactgt ccttctggtg attttgctta atttctgatg ttcgagacat
    36001 aattgaggag atttgtcttg attttttcct aaaaaagggt agactcctcc cttggtttga
    36061 tgctcgattt ttaaaataag gtgacttatt ctgttaggca tgttttcccc tgccctctag
    36121 ctctatatgg aacagggagt atcagattta tctggcagat tgtatcaaat aaggcttgaa
    36181 actgttttga ataggcgtat agacttgaga atttgagaat atgtcgtcta gcagtagaga
    36241 catattttcc ggcaatatga aggaagagac gtcgaaaacg cttgatagtc aaggccttta
    36301 cttcatcacc agctagctgc tttaaaaaga ggtagagatt ataagccaga cagcccatca
    36361 tcatacgtac ttcattctta atcatggtcg aactatctgt cttatcccca aagaatcctg
    36421 ctttcctttc tttgataaag ttttcagcat tccctcgttc acgatagaat tgaaacagct
    36481 cctcactatc tacactgctt aaattggtca aatgaacttt tttacaacca taaaaaagag
    36541 cacttaatcg tactcttctc ttttaaggat tagttgaatt aattcaaatc cactagttct
    36601 cctgtttcta ggtagactag gcgttcacaa atgttagcaa tgtaatcagc gaagcgttcc
    36661 agatgcccta tgatataaag gtattgtgtt ccattgggaa ttgaggtttc ttggtctttc
    36721 ataagtccaa tgatttcctt tgataaggca taataatatt ggtcaatctg ttcatctttt
    36781 tgagcaatac taatagcttt tgaggcttgg tgcaaaggaa aggcaaccaa taaatcggct
    36841 agcatgctga gggataattt acccatttgg tgtaactgtt cttcgtcagg ggctagttga
    36901 ttttctttta gttgcaaaac agctttggca atgcctgcca tatggtctcc catacgttca
    36961 aggtctgaac aagaagacat gatgctaatc acaaatcgaa ggtcagacac ttgtggctgc
    37021 tgcaaggcca acaaacgggc acaggtcaat tcgatagcgc tttgaccttg gttgatagca
    37081 tgatccttat tgataattag ctctgccatc tccttgtctt tggaggctaa ggccagtaag
    37141 gcttttgaag ctgtttcaag gacaagttgc cctagtccta aaaaggattg ttctaattca
    37201 tgcaattcta agtcaaattg atttctcata ggtttttcct tccttatccg aaccgtcctg
    37261 aaatatagtc ttctgtgcgc tgatcttttg gattggtaaa cacgtcaacg gtatctccaa
    37321 attcgcaaat ttctcctgtt aagaaaaaag cagttttatc tgaaatacgt gaagcttgtt
    37381 gcatgttatg ggtaacaatg ataatcgtat aatccttttt tagttgctga atgaggtctt
    37441 caatttttaa agtggagata gggtctaagg ctgaagtcgg ctcatccatt aacagaatat
    37501 caggttctac tgctaaagct cgcgcaatgc aaaggcgttg ctgctgaccg ccagataagg
    37561 acatggcact ctttttaaga tcatctttga cttcttccca aatggctgcc ccttttaaag
    37621 atttctccac taaggcatct aattgttttt tgtctcgaat accatgtgtc cttgggccat
    37681 aagccacgtt atcatagata gacatggcaa agggattagg ctgttgaaaa accatcccta
    37741 cacgcttacg tagctgatta aggttgaatt tgctactata aatatcttgc tcatctaaga
    37801 ggacttggcc ttcaatatgg caagaaggaa ccaaatcgtt catccggtta agggttttta
    37861 gaaaagttga tttgccacaa ccagatgggc ctatcaaggc agtaatctgt ctttctggta
    37921 attgaatcga aatatttttt aaggcttgaa aatccccgta aaataagtct aggtgtctga
    37981 ctgaaaatgt tcccatacta ggaagctcct ttcacaagtt ttcgagataa taagcttgat
    38041 agagtattta tcattaaaac agtaataatc aaaatcacgc cggtagcata ggcttcattg
    38101 acatgtagcc cctcacttga cagcatatac atatgtaggg ctagagaacg gcctgaagac
    38161 atgagactac ttggcgtatt ggtagaggta cctaatgtat acatgagggc agctgtttca
    38221 ccaacgatac ggccaatagc tagtatcact ccagctaaaa tacctggcat ggcaactggt
    38281 agaacaattc taaaaacagt ccgtaactta cctgccccaa gtccataact tgcttgacgc
    38341 atgctatcac taacagataa aagggcttct tctgttgagc gaataatgac tggcaacacc
    38401 atgatgactg aggttaagat tcctgataac agagagtatt gaaaacctaa gaagactaca
    38461 aagaagagca tgccaaacag accaaaaaca atggaaggaa tcccagacaa ggtatctgag
    38521 gccaatcgca tgattttaac acaaagggaa tctttttttg tatattccac aagataaaaa
    38581 ccagcaaaaa tccctatggg caaggctaaa agaagagcac caaagaccag aataacggtg
    38641 gaaataatcg ctggcataag ggaaatgttc tcagaagtat aagtccaaga aaagagggat
    38701 agacttagat gaggtaagcc tttgatgagg ataaaaccaa tgattaaaaa gagagagcca
    38761 aaggttaaag ctgaaaaaca ataaacgaga agttttagca ggtatttact cataagatga
    38821 ttttcctttc aagtaggcaa agtaggcatt aatcaagaga ataaggaaaa agagaactgc
    38881 tgaggttgca ataagggctt ccctatgctg acctgatgcg taagccattt ccagaacaat
    38941 attggttgtt aaggttctgg ttcctgaaaa gagtccactt ggaataatcg gctggttgcc
    39001 tgccaccaaa ataactgcca tggtttcacc tactgcgcga ccgattccta aaataactgc
    39061 tgataaaata ccagatctcg cagctggcaa gatgacacta aaaatactcc gttcatgact
    39121 agctcctaga gccaagctac cagaataata cgttttggga actgttcgga tagcagattc
    39181 tgacaaactg ataatggttg gcaaaatcat tattcctaat agtaacgaag cggttaggac
    39241 actcatgcca tttcctaaaa agcttctaat ccaaggcacc aataattgta ggccgaaaaa
    39301 accataaaca atagatggaa tggccgccat caagttgata gctgatttta agaagccata
    39361 gacgggcttt ggacaataat aaaccataaa caccgatgtc aagatgcctg ttggcacccc
    39421 aatcacaatc gctcctaagg taattaataa ggaaccaacg atcattggta aaataccata
    39481 gcttgccgga atgttcgttg gcgaccaatc actgcctaat aaaaaacggg caaagccgta
    39541 gttagccatg aaaggtaagc cattactaaa aataaagaaa cagattagca aaatagctac
    39601 aacagctact gttgcactca tgaaaaaaat tgccctaaaa actgcttctt tgaaggcttg
    39661 ttttgtcaca tcttgtcctt tctagtgaag aaagtaaggg agatacgaca cctccctact
    39721 tgccttcttt atcttattgt acgatgaaac gtctgcatct ctttagagat ttatggagca
    39781 aacattttat ttaatcttgt cccaggtggt taatttgcca ctaaaaacgt ctgcaagttc
    39841 agccatactg acttggcttg ccttattgtc attattgacc acaacagcaa taccgtctaa
    39901 agcaatagca tcatgggtga gactcttacc ttcttcagga gttaattccc tagaaaccat
    39961 accaatatca gcggttttct ccttaacagc ggtaatacct gctgaagacc cattagaggt
    40021 aatatcaatc gtaacttctg gattttcttt tttataagct tctgctaatt tttccattaa
    40081 agaagatact gaagtggaac ctacaacaga caacttgcct gataagtgtt ggcttgtata
    40141 ttctgtggtt tcggttttag cttcaataaa tttattatct gtgaccactt gttgaccttg
    40201 tttggagtgg ataaagctga taaaatcttg acctagcttg gaaagattag aagaccaaac
    40261 aatgttgaag ggacgttgaa gagggtattc accatctaaa actgtgtctc gactagcctt
    40321 gacaccatca atctctaaag ccttgacaga tttcgttaaa gatcccaagg agatgtagcc
    40381 gatagcatta gcattccctt gaactgctga gagaacacct tctgtactat tttgaatcac
    40441 agctgttttg gcagtgttgt caattttttt atcaccgtct tttttgagaa tccctgtgat
    40501 ttctgtgaag gcaccccgtg ttccagagcc attttctcgt gaaatcacct caatcgttcc
    40561 tgaagctgac tgtttggaag cagctgactg attgccacag gcaacaagcc caaatcctga
    40621 taagccaatg gctgcaagag taagcatttt tttgaatttc ataataatca cctttatctc
    40681 tatgtatttt tcttgtgtag gcttactaca tttatagtct aacaagtctt tgtaaaggtt
    40741 tatccctgat tcatgtaaag attgtgtaaa gaatcaaaaa aagccacttt tgaaaaatgg
    40801 ctgctcctaa aaatagcttt aaaaattatt agtcctgtgc gaaagattgg ttaggaagaa
    40861 aaatcgtgaa gcaactgcct ctgccaagct gactcgtcac cgtgacttgg ccacctaata
    40921 attgactgag ttctttgaca atggcaaggc caagaccagt gccaccagtt tgtctgcttc
    40981 gacctttatt aactcggtaa aaacgttcaa aaatacgatc ctgctctaat tgactaatac
    41041 caatccctgt atctgataca gaaatcttaa tgccttcgtt caccttttgg gtcttgacct
    41101 caattttccc cccttgttca gtgtaacgga tggcattgga taaaagattg agtaagattt
    41161 gggaaagtaa ttgactatct gatacgaggg tgacatcatc tggcacctgc acctttagct
    41221 gtaaatcctt cttcttgagc tgaggttgca agctttgagt caaatcctgt acaaattctg
    41281 ccaaagaaag ggtcgtccat tgtataggca tttgttgagc cttagataag gtaagaagat
    41341 gctcaacaat atgctcaaga cgcaaacttt ctttgtaaat aatgtctaga aagtcatcct
    41401 tgagcgcttc ttcttcagct gacatcccct taatggtttc agcaaagccc ttaatcgaag
    41461 taactggtgt cctcaattca tgggaggcat ttgagacaaa ggctaaattt aacttttcat
    41521 aagttctaat cgttgttaaa tcatatagca agacgagcac agcttccaca gattgggtgg
    41581 ggctaaaaac gggaactgct gtcacttcta aaatcaagtc accctcatga aacccactta
    41641 cttcttgttt taaccttgtt ttttgatcaa aggcttggtg aactaaattc cgaatatcca
    41701 tccgtttgag gtcatcaagt gaacttatgt cgccgtccac atcgggaaaa taatgaggca
    41761 gagagcgact ggataataac atctgacctt gagcggaaac taaaaacgtc cccatggtta
    41821 ggtgcgacag aagaacctcc attgtttcgg ctagatcctt gtattgctga tcctgttggg
    41881 agactttggt ttttaggcca gacacatact gagccaaaga ctttaagtct tcttgccctt
    41941 tttctaaaaa gtattcacta ctggtcaaga gaggttggtg caaggtctca aaagcaactt
    42001 cccatttcca aaggcaaaag agccagtagc cacctagtcc caaagaaagg gctagaagaa
    42061 agagaccgat gcctttactg atccaagtta atgccatccc tgcaatcaga atgaggctaa
    42121 cacttagatt gactagccaa aattgaaggt agcgtttcat ctataactcc ttgaacttat
    42181 aaccataacc ccgaatggtt cgaataaatt gaggggcttt aggattgtct tcaatttttt
    42241 ccctcaactt accaatatga acgtccacca aacgtgtttc ctgcccaaag tcataccccc
    42301 agatacgttc caaaagacgc tctctagtca gtgtcatgtt gggatgtttc ataagataga
    42361 gcaagagttc aaattctttt ggggtcaaac tcagtaactt attcgccttg tagacttcat
    42421 gacgctcagg gtatactttc aaggtcccaa atagccaaga atcgtcagcg atattatctg
    42481 aatcatctcc ttcttgttct cctttagttc gcctgaggac agccttgaca cgcgccagca
    42541 attctctagg gctaaaaggc ttggtcaggt agtcatcagc ccctaattcc aaggccaaaa
    42601 ccttatcaaa ttcatcactt ttcgcagaaa ccatcataat tggagttttg acgcctttgg
    42661 ctctcagccg cttacaaact tctatgccat ctaattgtgg taacatgata tcaagcaaga
    42721 taaaatcaaa gggttctgtt tctgccaaag ctaaggcctt ccgtccattt gtcaccaatt
    42781 gagtagaaaa gccttcctta cttaaatggt agtcaagcaa tttcagaatg tgttcttcat
    42841 catccactaa taagacttgt tttgtcatct attatctcct attggtaaca ttataacaca
    42901 attatcagaa atcctaacat tgctaaatca gattaaattt gcctatcaag actagtatct
    42961 ggtcaaacgc tcaatcatct ccttgtgctc tggataggtc gccagtagat ctaccctttc
    43021 aaataattca aaatcctcaa attcaaaacc aggagcaaca agacaagaaa ccagagcatc
    43081 atccttatca actgttgatc cccaaatagt gcccttagga acacagtagt gaagttgttg
    43141 ccctttggat atgtccaggc ctaaagtgac tgcttcgtag tgaccatctg ctgtaatcat
    43201 gtgaacagta agtggggatc ctgcatgaaa ataccagatt tcatctgctg tcaatcggtg
    43261 aaaatgtgaa ggattcgttt cttctaataa gaaataaata ctggtataaa gcgcccttcc
    43321 cttaccagca aggtttatag tgtctgaagc tttttttgtt tgtctaaaat agccaccttc
    43381 aacatgggga gctaactcta gagttcttat caagtcttct ttatccgtcg gagccaatgg
    43441 gttgaagtaa ctcttgttca aagtggtttt acgatttcaa gaactcctct cagttctgag
    43501 gacacggtaa tgattgatgc gacggaagta caaatcaatc gccctaaaaa aagaattagc
    43561 gaattattct gataaaaaaa atgccacgct atgaaggctc aagcgattgt cacaagtcaa
    43621 gggagaattg tttctttgga tatcgctgtg aactattgtc atgatatgaa gttgttcaaa
    43681 ataagtcgca gaaatatcgg acaagctggt aaaatcttgg ctgacagtgg ttatcaaggg
    43741 ctcatgaaga tatatcctca agcacaaact ccacgtaaat ccagcaaact caagccgcta
    43801 acagttgaag ataaagccta taaccatgcg ctatccaagg agagaagcaa ggtcgagaac
    43861 atctttgcca aagtaaaaac gtttaaaatg ttttcaacaa cctatcgaaa tcatcgtaaa
    43921 cgcttcggat tacgaatgaa tttgattgct ggtattatca atcatgaact aggattctag
    43981 ttttgcagga agtctattat ttggttaggt gaattagtga agcgtttagg caagtgtctc
    44041 tggttacgac gtcatggcct ctaaatcgat tatatttagg ggtcatgact agtgaagcag
    44101 ttagctagtt cgcatataag cggctagcgt ctaacaatta ggaactttag ttccaataac
    44161 tttaagatta cgacgtttta ggacataaat cgatcatatt tatgtcctaa aactagtgaa
    44221 gcgcctagcc aaagtccgaa taggatttgg cgttagttac ttagattgct ttgcaatcaa
    44281 gtaactttgg cgatttacat cttctctggc gcttctactc caagcaagcg aagggcttct
    44341 ttgagaacga ctgcggttgc gtagctgagg gctagacggc tgtcgcgttc tgggctttca
    44401 tccaagatac gtgtatgtgc atagtatttg ttaaaggatt gagccaggct aattgcaaat
    44461 ttagcaatga tagaaggttc aaagttatct gccgcacggt tgataatacg tgggaagtct
    44521 tgaatgagtt taatgatttc ccagctttca gtatcattca agctatagtt gccagctgtt
    44581 tctggtttga aatcggcttt gcgtaagata gattggatac gagcgtaggc atattgaacg
    44641 taaggtccag tttcaccctc gaaggatacc atagcttcta ggtcgaagtc gtatccattt
    44701 gtacggtcgg ttttgaggtc atagaattta atggctccaa ccccaacagc atgtgctact
    44761 tggtctttgt tttctagttc aggattttta gcctcgattt ggaccttggc acggctaaca
    44821 gcctctgcaa cagtaggctc tagcaagatg acattccctt tacgagtaga gagcttcttc
    44881 ccttcttttg taaccagacc aaaaggaacg tgggtaatgt cgtcactcca atcgtagccc
    44941 atctcttgca agacagcttt gagctgttta aagtgggcag attgttcttg accaacgaca
    45001 tagatagatt tagcaaattg gtattcgttt ttacggtaga gggctgcagc caagtcacgt
    45061 gtgatataga gagttgcacc atcagacttc ttgatgaggg ctggatgttc aattccatat
    45121 ttctcaagat tcacaacttg ggcaccttct gattcaagaa gtagtccttt ttcagaaaga
    45181 atgtctacaa ctgcatccat cttatcattg tagaaggctt ctccgttata gctgtcaaat
    45241 tcaaccttca attcattgta aaggcggtta aattccacta aactttcatc gcggaaccat
    45301 tgccaaagag cgagagcttc ctcatctcca ttttcaagtt tacggaacca ttcgcgcgct
    45361 tcttcatcca agctagggtc attttcagct tcagcgttga tgcggacata gagtttaagg
    45421 agttcatcga ttggatgagc ttttacagct tcttcgtcgc cccatttttt gtaggcaaca
    45481 atcaacatcc caaattgttt accccagtct cccaaatggt tgaccttgac cgtttgataa
    45541 ccgatttttt ggaaaatatg tgacaagcta tctccgataa cagttgaacg caggtggcca
    45601 atagaaaatg gtttagcgat attcggacta gacatgtcga taacaacatt ttcttgtttg
    45661 ccaatatttt ggtcagcata gtgttctttt tcagtggtaa cagcttgcaa tacttgagca
    45721 gaaatggcag atttatcaag gaaaaagtta acgtaaggtc ctgttgcgac aactttttca
    45781 aaggcttggc tgttcatttt ttcagccagt tcagccgcaa tcatttgtgg tgctttacgt
    45841 tcgacttttg caagagaaaa agcagggaaa gcaatgtctc ccatttctga gtttttaggg
    45901 gtttccagta actttaaaat agcctcttgg tccaggctat caatgatgct agataattcg
    45961 ctagcaatca attcttttgt attcattaag agctcctttt tggacttttc tactatttta
    46021 tcacaatttt aaagaaagaa gaaaaaattt ttgaaatctc ctgttttttt ggtataatat
    46081 ggttataaat atagttataa atatgcacgc aagaggattt tatgagaaaa agagatcgtc
    46141 atcagttaat aaaaaaaatg attactgagg agaaattaag tacacaaaaa gaaattcaag
    46201 atcggttgga ggcgcacaat gtttgtgtga cgcagacaac cttgtctcgt gatttgcgcg
    46261 aaatcggctt gaccaaggtc aagaaaaatg atatggtgta ttatgtacta gtaaatgaga
    46321 cagaaaagat tgatttggtg gaatttttgt ctcatcattt agaaggtgtt gcaagagcag
    46381 agtttacctt ggtgcttcat accaaattgg gagaagcctc tgttttggca aatattgtag
    46441 atgtaaacaa ggatgaatgg attttaggaa cagttgctgg tgccaatacc ttattggtta
    46501 tttgtcgaga tcagcacgtt gccaaactca tggaagatcg tttgctagat ttgatgaaag
    46561 ataagtaagg tcttgggagt tgctctcaag acttattttt gaaaaggaga gacagaaaat
    46621 ggcgatagaa aagttatcac ccggcatgca acagtatgtg gatattaaaa agcaatatcc
    46681 agatgctttt ttgctctttc ggatgggtga tttttatgaa ttattttatg aggatgcggt
    46741 caatgctgcg cagattctgg aaatttcctt aacgagtcgc aacaagaatg ccgacaatcc
    46801 gatccctatg gcgggtgttc cctatcattc tgcccaacag tatatcgatg tcttgattga
    46861 gcagggttat aaggtggcta tcgcagagca gatggaagat cctaaacaag cagttggggt
    46921 tgttaaacga gaggttgttc aggtcattac gccagggaca gtggtcgata gcagtaagcc
    46981 ggacagtcag aataattttt tggtttccat agaccgcgaa ggcaatcaat ttggcctagc
    47041 ttatatggat ttggtgacgg gtgactttta tgtgacaggt cttttggatt tcacgctggt
    47101 ttgtggggaa atccgtaacc tcaaggctcg agaagtggtg ttgggttatg acttgtctga
    47161 ggaagaagaa caaatcctca gccgccagat gaatctggta ctctcttatg aaaaagaaag
    47221 ctttgaagac cttcatttat tggatttgcg attggcaacg gtggagcaaa cggcatctag
    47281 taagctgctc cagtatgttc atcggactca gatgagggaa ttgaaccacc tcaaacctgt
    47341 tatccgctac gaaattaagg atttcttgca gatggattat gcgaccaagg ctagtctgga
    47401 tttggttgag aatgctcgct caggtaagaa acaaggcagt cttttctggc ttttggatga
    47461 aaccaaaacg gctatgggga tgcgtctctt gcgttcttgg attcatcgcc ccttgattga
    47521 taaggaacga atcgtccaac gtcaagaagt agtgcaggtc tttctcgacc atttctttga
    47581 gcgtagtgac ttgacagaca gtctcaaggg tgtttatgac attgagcgct tggctagtcg
    47641 tgtttctttt ggcaaaacca atccaaagga tctcttgcag ttggcgacta ccttgtctag
    47701 tgtgccacgg attcgtgcga ttttagaagg gatggagcaa cctactctag cctatctcat
    47761 cgcacaactg gatgcaatcc ctgagttgga gagtttgatt agcgcagcga ttgctcctga
    47821 agctcctcat gtgattacag atgggggaat tatccggact ggatttgatg agactttaga
    47881 caagtatcgt tgcgttctca gagaagggac tagctggatt gctgagattg aggctaagga
    47941 gcgagaaaac tctggtatca gcacgctcaa gattgactac aataaaaagg atggctacta
    48001 ttttcatgtg accaattcgc aactaggaaa tgtgccagcc cactttttcc gcaaggcgac
    48061 gctgaaaaac tcagaacgct ttggaaccga agaattagcc cgtatcgagg gagatatgct
    48121 tgaggcgcgt gagaagtcag ccaacctcga atacgaaata tttatgcgca ttcgtgaaga
    48181 ggtcggcaag tacatccagc gtttacaagc tctagcccaa ggaattgcga cggttgatgt
    48241 cttacagagt ctggcggttg tggctgaaac ccagcatttg attcgacctg agtttggtga
    48301 cgattcacaa attgatatcc ggaaagggcg ccatgctgtc gttgaaaagg ttatgggggc
    48361 tcagacctat attccaaata cgattcagat ggcagaagat accagtattc aactggttac
    48421 agggccaaac atgagtggga agtctaccta tatgcgtcag ttagccatga cggcggttat
    48481 ggcccagctg ggttcctatg ttcctgctga aagcgcccat ttaccgattt ttgatgcgat
    48541 ttttacccgt atcggagcag cagatgactt ggtttcgggt cagtcaacct ttatggtgga
    48601 gatgatggag gccaataatg ccatttcgca tgcgaccaag aactctctca ttctctttga
    48661 tgaattggga cgtggaactg caacttatga cgggatggct cttgctcagt ccatcatcga
    48721 atatatccat gagcacatcg gagctaagac cctctttgcg acccactacc atgagttgac
    48781 tagtctggag tctagtttac aacacttggt caatgtccac gtggcaactt tggagcagga
    48841 tgggcaggtc accttccttc acaagattga accgggacca gctgataaat cctacggtat
    48901 ccatgttgcc aagattgctg gcttgccagc agacctttta gcaagggcgg ataagatttt
    48961 gactcagcta gagaatcaag gaacagagag tcctcctccc atgagacaaa ctagtgctgt
    49021 cactgaacag atttcactct ttgatagggc agaagagcat cctatcctag cagaattagc
    49081 taaactggat gtgtataata tgacacctat gcaggttatg aatgtcttag tagagttaaa
    49141 acagaaacta taaaaccaag actcactagt taatctagct gtatcaagga gacttctttg
    49201 acaattctcc acttttttgc tagaataaca tcacacaaac agaatgaaaa ggagctgacg
    49261 cattgtcgct cccttttgtc tattttttaa ggagaaagta tgctgattca gaaaataaaa
    49321 acctacaagt ggcaggcctt ggcttcgctc ctgatgacag gcttgatggt tgctagttca
    49381 cttctgcaac cgcgttatct gcaggaagta ttagacgccc tccttactgg gaaatatgaa
    49441 gctatttata gtatcggggc ttggttgatt ggtgtggccg tagtcggtct agttgctggt
    49501 ggactcaatg ttgtcctcgc agcctatatt gcccaaggag tttcatccga ccttcgggag
    49561 gatgccttcc gtaaaattca aaccttttct tatgctgata ttgaacaatt taatgcggga
    49621 aatctagtcg ttcgaatgac aaatgatatc aaccagattc agaacgttgt catgatgacc
    49681 ttccaaattc ttttcagact tcccctcttg ttcatcggtt cgtttatcct agcggttcaa
    49741 accttacctt ctctgtggtg ggtgattgtt ctcatggtag tcttgatttt tggtttgact
    49801 gctgtcatga tgggaatgat ggggcctcgt tttgccaagt ttcaaaccct tcttgagcgc
    49861 atcaatgcca ttgccaagga aaatttacgt ggcgttcgtg tggtcaagtc ctttgtccaa
    49921 gaaaaagagc aatttgctaa gtttacagag gtctcagacg agctttttgg tcaaaacctt
    49981 tacattggtt atgccttttc agtagtggaa ccctttatga tgttggttgg ttacggggcg
    50041 gtcttcctct ctatttggct ggtcgcggga atggttcagt cggatccgtc tgttgttggt
    50101 tccatcgctt cttttgttaa ttacctaagc cagattatct ttaccattgt tatggttgga
    50161 tttttgggaa attctgtcag ccgtgccatg atttccatgc gtcgtattcg agaaattctt
    50221 gacgcagagc cagctatgac cttcaaggat atcccagatg aagagttggt tggaagcctt
    50281 agctttgaaa atgtgacctt tacctatcca atggacaagg aaccgatgct gaaagatgtg
    50341 agctttacta ttgaacctgg tcaaatggtt ggtgtagttg gagcgactgg tgcaggaaag
    50401 tcaaccttgg ctcaattgat tccacgtctc tttgatccac agaacggggc cattaaaatc
    50461 ggtggcaagg atattcgaga agtgagtgaa ggaaccctgc gtaaaacagt ttccatcgtt
    50521 ctccaacgtg ccattctttt tagtggaacg attgcagata acttgagaca ggggaaggga
    50581 aatgctactc tatttgaaat ggagcgcgca gccaatattg cccaggctag tgaattcatt
    50641 catcgtatgg agaaaacctt tgaaagtcca gttgaagaac ggggaaccaa tttctctggt
    50701 ggacaaaaac aaaggatgtc gattgcgcgt gggattgtca gcaatccacg tattctgatt
    50761 tttgatgatt cgacctcagc cttggatgcc aaatcagagc gcttggtgca agaagctttg
    50821 aataaggact tgaaggggac gacaaccatt attattgctc aaaaaattag ctcggttgtc
    50881 catgcagaca agatcttggt tctaaatcaa ggacgattga ttggtcaagg tacgcatgca
    50941 gacttggttg ccaacaatgc cgtttaccgt gaaatctatg aaacacagaa atgaaagaca
    51001 aactataaga aaagtcaata gttttatcta aactatttct tatttcaatt tgatgatttg
    51061 gcgatgattt tagagcacgg caaaaggccc ttgaaaaagt ccattttttc aaaggtaatc
    51121 ctgtgttaat ttcagaaatt acatcacttt ttgttcgtca aatggcagct ctttttttag
    51181 gatataaaac agggttcgga taagtttttt tgcaaggtgg atgatggcta cattgtaatg
    51241 ttttccttgt tctaatttag tcttaagata ggccttaaaa gcaggcgaaa agcgagggca
    51301 tactttggca gcttgtatga gtacctaccg cagatgaggg gaactccgtt tgaccattct
    51361 tcctgctaaa tcaatctgat ctgactgata aatagaagaa tccagtccag cgaaagcttg
    51421 taattgagca ggattatcaa aggcatgaat atttcgaatc tcagctaaaa tgaccgcccc
    51481 taaacgatcc ccaatcccag taaccgtcgt gatgaccgag ttgaactcag ccatcaagtc
    51541 attgacacat ttttccgcct tgtcaatgag cctcttgtaa tgtttgatgt tttcattaca
    51601 cgagataaaa cgtctatgcg ttatcaaact cattaccaat taaaacaaat gtggttagat
    51661 cctttcggaa attgtcaagc gattggagga aatgaactaa tccacagcgg cttattccaa
    51721 gtataccact tgggctttgg cagtagctaa ctgcgctaaa tataatataa ggaggagtaa
    51781 aatgaagaca gttcaatttt tttggcatta ttttaaggtc tacaagttct catttgtagt
    51841 tgtcatcctg atgattgttc tggcgacttt tgcccaagcc ctctttccag tcttttctgg
    51901 acaagcggtg acgcagctag ccaatttagt tcaagcttat caaaatggca atccagaact
    51961 tgtatggcaa agcctatcag gaatcatggt caatcttggc ctgctggttt tggttctatt
    52021 tatctctagt gtaatataca tgtgtctcat gacgcgcgtg attgcagaat cgaccaacga
    52081 gatgcgcaaa ggtctctttg gtaagcttgc tcagttgacg gtttctttct ttgaccgtcg
    52141 acaagatggc gatatcctgt ctcattttac cagtgatttg gataatatcc tccaagcctt
    52201 taacgaaagc ttgattcagg tcatgagcaa tattgtttta tacattggtc tgattcttgt
    52261 catgttttcg agaaatgtga cgctggctct catcaccatt gccagcaccc cattggcttt
    52321 ccttatgctg attttcatcg tgaaaatggc acgtaaatac accaacctcc agcagaaaga
    52381 ggtagggaag ctcaacgcct atatggatga gagcatctca ggccaaaaag ccgtgattgt
    52441 gcaaggaatt caagaggata tgatggcagg atttcttgaa caaaatgagc gcgtgcgcaa
    52501 ggcaaccttt aaaggaagaa tgttctcagg aattcttttc cctgtcatga atgggatgag
    52561 cctgattaat acagccatcg tcatctttgc tggttcggct gtacttttga atgataagtc
    52621 tattgaaaca agtacagccc taggtttgat tgttatgttt gcacaatttt cacagcagta
    52681 ctaccagcct attatccaag ttgcagcgag ttggggaagc cttcagttgg cctttactgg
    52741 agctgaacga attcaggaaa tgtttgatgc agaggaggaa atccgacctg aaaaggctcc
    52801 aaccttcact aagttgcaag aaagtgttga aatcagtcat atcgattttt catacttgcc
    52861 tgataaacct attttgaaag atgtcagcat ttctgcccct aaaggccaga tgacagcagt
    52921 tgttgggccg acaggttcag gaaaaacgac tattatgaac ctcatcaatc gcttttatga
    52981 tgttgatgct ggtggtattt attttgatgg taaagacatt cgtggctatg acttagatag
    53041 tcttagaagc aaggtgggaa ttgtattgca agattcggtc ttgtttagcg gaacgattcg
    53101 agacaatatc cgatttggtg tgccagatgc tagtcaggaa atggttgagg tagcagcaaa
    53161 agcaacccac attcacgact atatcgaaag tttgcctgat aagtacgata ctcttattga
    53221 tgatgaccag agcatctttt caacagggca gaagcaattg atttcaatcg ctcgaaccct
    53281 gatgacagat ccagaagttc tcattctcga tgaagcaact tcaaacgtag atacggtgac
    53341 agaaagcaag attcagcatg ccatggaggt ggttgtagca ggtagaacta gtttcgtcat
    53401 tgcccaccgc ttgaaaacca ttctcaatgc agatcagatt attgtcctta aagatggaga
    53461 agtcattgaa cgtggtaacc accatgaact tttgaagcta ggtggctttt attcagaact
    53521 ctatcacaat caatttgttt tcgaataaga aagaagttgt cctatgtggg cagctttttc
    53581 ttgtccataa aaaatgttta tcacagcctt aaaaaaaaca tattagacga aagtcatttt
    53641 gagtgatatg ataggactat cgttagcatt cgaaaggaga ggcatcatgg ctagaacggt
    53701 tgtaggagtt gctgcaaatc tatgtcccgt agacgcagaa ggtaaaatca ttcattcatc
    53761 tgtatcttgt agattcgcag agatcattcg tcaagtcggt ggtctccctt tagtcattcc
    53821 tgttggtgat gagtcagttg tacgtgatta tgtggaaatg attgacaaac tcattttgac
    53881 aggaggccaa aatgttcatc ctcagtttta tggagagaaa aagaccgtcg agagcgatga
    53941 ttacaatctg gtccgtgacg aatttgaatt ggcactcttg aaggaagcgc ttcgtcagaa
    54001 taaaccaatt atggcaatct gtcgcggtgt ccaacttgtc aatgttgcct ttggtggaac
    54061 cctcaatcaa gaaatcgaag gtcactggca aggactacct ttcgggacat ctcactctat
    54121 tgagacagta gaaggaagcg tggtggctaa gctatttggg aaagaaagtc aggtcaattc
    54181 agtccatcgt caaagcatta aagatttggc acccaatttc cgtgtaactg ctattgattc
    54241 aagagaccaa actattgaag cgattgagtc tatcgacgag caccgcatta tcggtttaca
    54301 gtggcatcca gagtttctgg ttaatgaaga agatggcaat ttagaattat ttgagtattt
    54361 attgaatgaa ctgtaacgat tggaacatct ggtcgttctt tcttttattt tttcaaaatt
    54421 tttagaatgt ggtattttac gcaaacgttt gaattctgat aaaaattgga gaaattcgac
    54481 aaaaaaactt gaaaaaaacg aaggtaagcg ttatgataga aaagaagaaa tattggagga
    54541 agaacatgtc acatattaaa tttgattatt caaaagtttt agacaaattt gttgcaccac
    54601 atgaagtgga atacatgcaa tcacaagtaa cagcagcaga tgaattgatc cgtaaaggaa
    54661 ctggtgctgg tagcgacttc ttgggatggt tggaccttcc tgaaaaatac gatcgcgaag
    54721 aatttgaccg catcttgaaa gctgctgagc aaatcaagtc agacagcgat gttttggttg
    54781 tgatcggtat tggtggatct taccttggtg caaaagcagc aatcgacttc ttgaatcacc
    54841 attttgctaa cttgcaaaca aaagaagaac gcaaagctcc acaaatcctt tacgcaggaa
    54901 actcaatctc atctacctac cttgctgact tggtagagta tgttgcagac aaggacttct
    54961 cagtaaacgt aatttctaaa tcaggtacaa caactgaacc agcgattgct ttccgtgtct
    55021 ttaaagaact cttggttaag aaatacggtc aagaagaagc taacaaacgt atctatgcaa
    55081 caactgaccg ccaaaagggt gctgttaagg ttgaagcaga cgctaacggt tggggaacat
    55141 ttgttgttcc agatgatatc ggtggacgct tctcagtatt gacagccgtt ggtttgcttc
    55201 caatcgcagc atcaggagct gacatcaaag ctcttatgga aggtgcgaat gcagctcgca
    55261 aagactacac ttcagacaaa atctctgaaa acgaagctta ccaatacgca gccgttcgta
    55321 acatccttta tcgtaaaggc tatgcaactg agatcttggt aaactatgag ccatcacttc
    55381 aatacttctc agaatggtgg aaacaattgg ctggtgaatc agaaggaaaa gaccaaaaag
    55441 gtatctaccc aacttcagcc aacttctcaa ctgacttgca ctcacttggt caatttatcc
    55501 aagaaggaac tcgtatcatg tttgaaacag ttgtccgtgt tgacaaacct cgtaaaaacg
    55561 tgcttattcc tactttggaa gaagaccttg acggacttgg ttaccttcaa ggaaaagacg
    55621 ttgactttgt aaacaaaaaa gcaactgacg gtgttcttct tgcccacaca gatggtgatg
    55681 taccaaacat gtatgtgact cttccagagc aagacgcttt cactcttggt tacactatct
    55741 acttcttcga attggcaatt gccctttcag gttacttgaa tgctatcaac ccatttgacc
    55801 aaccaggtgt tgaagcttat aaacgtaaca tgtttgccct tcttggaaaa ccaggatttg
    55861 aagaattgag caaagaactt aacgcacgtc tataatagaa gaaaagagtg gtttgcccac
    55921 tctttttact ctctttatcc atagaaattg gactcagcca agacttgtga tataatatag
    55981 aaagcaaaaa ggcagacgcc tagataatag gagaaactat gtcaaaagat atccgcgtac
    56041 gttacgcacc aagtccaaca ggactactac acatcggaaa tgctcgtaca gcattgttta
    56101 attacttgta tgcgcgccat catggtggaa catttctcat ccgtatcgaa gatactgacc
    56161 gtaaacgcca tgtcgaggat ggtgaacgtt cacaacttga aaaccttcgc tggttaggca
    56221 tggattggga tgaaagtcca gaatcacatg agaattatcg ccagtctgag cgtttagact
    56281 tgtatcaaaa atacattgac caactattag ctgaaggaaa agcctataaa tcttacgtta
    56341 cagaagaaga gttggcagct gaacgcgaac gccaagaagt agctggcgaa acaccacgct
    56401 acatcaatga ataccttggt atgagtgaag aagaaaaagc agcttacatc gcagaacgtg
    56461 aagcagcagg gatcatccca actgttcgtt tggctgtcaa tgagtcaggt atctacaagt
    56521 ggcatgatat ggtcaaaggc gatatcgaat ttgaaggtgg caatatcggt ggtgactggg
    56581 ttatccaaaa gaaagacggt tacccaactt acaactttgc cgttgttatc gatgaccacg
    56641 atatgcaaat ctctcatgtt atccgtggag atgaccatat tgctaataca ccaaaacagc
    56701 ttatggttta tgaagctctt ggttgggaag ctccagagtt cggtcacatg accttgatta
    56761 tcaactctga gactggtaag aagttatcta aacgtgatac taatacactt caatttatcg
    56821 aagattaccg aaagaaaggt taccttccag aagcagtctt taactttatt gctcttcttg
    56881 gttggaaccc aggtggtgaa gatgagattt tctctcgtga agaactcatt aaactttttg
    56941 atgaaaaccg cctcagcaag tcaccagcag cctttgatca gaaaaaacta gactggatga
    57001 gcaatgatta tatcaagaat gcagaccttg aaaccatctt tgaaatggca aaaccattct
    57061 tagaggaagc aggccgtttg actgacaagg ctgaaaaatt agttgagctc tataaaccac
    57121 aaatgaaatc agtagatgag attatcccat tgacagatct tttcttctca gatttcccag
    57181 aattgacaga agcagagcgc gaagtcatga cgggtgaaac agttccaaca gttcttgaag
    57241 cattcaaagc aaaacttgaa gcgatgacag atgatgaatt tgtgacagaa aatatcttcc
    57301 cacaaattaa agcagtccaa aaagaaacag gtattaaagg gaaaaatctt ttcatgccta
    57361 ttcgtatcgc agtttcaggc gaaatgcatg ggccagaatt accagataca attttcttgc
    57421 ttggacgtga aaaatcaatt cagcatatcg aaaacatgct aaaagaaatc tctaaataaa
    57481 gaggatacaa taatggacat ctgggaaaag atgtacgaag aagcacagaa actatacaat
    57541 ccacatgaag tatcagactt tgtttatgcc aatcatgttg tagctgcagt agaagcagga
    57601 gatggacaaa tatttacagg attctgtatg gagggaacct gtggtgtttt ccatctctgc
    57661 gcagaacggg cggccctctt caatatgtac caattttcag gacaaactaa ggttaagaaa
    57721 gtattagcct ttcgagacaa accaccttat ggtggaagtt cagccatgcc ttgcggtgct
    57781 tgtagagaat tccttttaga gcttaatgct gaaaataaag atgcagaatt catgatggac
    57841 tataatataa gaaaaacagt taaagtcgca gaactaatcc cttattggtg gggagaagaa
    57901 cgtgcttcta agtttaatga gcgatagaag agtcagtata attctggctt tttttactat
    57961 aagttgaaaa aata
//
LOCUS       DAVLVP010000004        56627 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.004, whole genome shotgun sequence.
ACCESSION   DAVLVP010000004 DAVLVP010000000
VERSION     DAVLVP010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 56627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 56627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56627
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.004"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(60..1022)
                     /locus_tag="VYN69_000206"
     CDS             complement(60..1022)
                     /locus_tag="VYN69_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEV1532563.1"
                     /translation="MPKKILVLHTGGTISMQADASGAVVTSSDNPMNHVSNPLEGIQV
                     HALDFFNLPSPHIKPKHMLVLYQKIKEEADNYDGVVITHGTDTLEETAYFLDTMEVPH
                     MPIVLTGAMRSSNELGSDGVYNYLSALRVASDDRAADKGVLVVMNDEIHAAKYVTKTH
                     TTNVSTFQTPTHGPLGLIMKQEILYFKTAEPRVRFDLDHIQGLVPIISAYAGMTDELI
                     DMLDLEHLDGLIIQAFGAGNIPKETAQKLESLLQKGIPVALVSRCFNGIAEPVYAYQG
                     GGVQLQKAGVFFVKELNAQKARLKLLIALNAGLTGQALKDYMEG"
     gene            1088..2476
                     /locus_tag="VYN69_000207"
     CDS             1088..2476
                     /locus_tag="VYN69_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000453140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1532564.1"
                     /translation="MEVKAVFFDIDGTLVNNRKSVLKSTKDAIKIVKEQGVLVGVATG
                     RGPFFVKELMDDLDLDFAVTYNGQYIFSKDRVLFTSPISKLHLRHLISYAKKEGTEIA
                     LGTKDAMLGSKIMSFGLGSFSQRISRFVPSVLTRTVSQSFNRMVSKVVPQKEEDLLHL
                     MNQPIYQVLMLMTPEESEKAAADFEDLKLTRSNPFASDVINQGNSKLEGIRRVGKEYG
                     FDLNQVMAFGDSDNDLEMLAGVGMSVAMGNGSSSVKEAAKHITTSNQQDGIHKALEHF
                     GVLSSEKVFVSRDYHFNKVKTFHHMMDERTQEEPRAWDLEGATHRAGFKIEELVEFVR
                     AASPSEEDFGQAVSQLHQALDKAADKVAKKTPAQQDLIGQVDALIDTLYFTYGSFVLM
                     GVDPERIFDIVHQANMGKIFPDGKAHFDPVTHKILKPDNWEEKYAPEPAIKKELQRQL
                     KAYERHKERNKS"
     gene            complement(2511..2963)
                     /locus_tag="VYN69_000208"
     CDS             complement(2511..2963)
                     /locus_tag="VYN69_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEV1532565.1"
                     /translation="MAQRYQNIMVAIDGSKEADLAFVKGVHSALRNDAKLTIVHVIDT
                     RALQSVSTFDAEVYEELQVDAESLMKEYEKRAKDAGVADVHIVIEMGNPKTLLARTIP
                     DAEEVDLILVGATGLNAFERLLVGSSSEYILRHAKVDLLVVREQEKTL"
     gene            3125..3418
                     /locus_tag="VYN69_000209"
     CDS             3125..3418
                     /locus_tag="VYN69_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009014348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532566.1"
                     /translation="MMKNSFQKSNFLYYGIILVSVLVEVIMIWQLESLVPLLYPSFIG
                     FLVFHVLYHLILFLVAKRSGRWDYLMIWGLFLMFNFLYDSFLGLLFLGLSFGM"
     gene            3574..4788
                     /locus_tag="VYN69_000210"
     CDS             3574..4788
                     /locus_tag="VYN69_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1532567.1"
                     /translation="MKEYNKSSKLEHVAYDIRGPVLEEAMRMRANGEKILRLNTGNPA
                     EFGFTAPDEVIHDLIMNARDSEGYSDSKGIFSARKAIMQYCQLKKFPNVDIDDIYLGN
                     GVSELIVMSMQGLLDNGDEVLVPMPDYPLWTAAVSLAGGNAVHYICDEAVEWYPDIDD
                     IKSKITSNTKAIVLINPNNPTGALYPKELLLEIIEIARQNDLIIFADEIYDRMVMDGH
                     VHTPVASLAPDVFCVSMNGLSKSHRIAGFRVGWMVLSGPKTHVKGYIEGLNMLSNMRL
                     CSNVLAQQVVQTSLGGHQSVDELLLPGGRIYEQRNFIYNAIQDIPGLSAVKPKAGLYI
                     FPKIDRNMYRIDDDEQFVLDFLKQEKVLLVHGRGFNWQEPDHFRIVYLPRVDELAQIQ
                     EKMTRFLKQYRR"
     gene            4933..5067
                     /gene="rpmH"
                     /locus_tag="VYN69_000211"
     CDS             4933..5067
                     /gene="rpmH"
                     /locus_tag="VYN69_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEV1532568.1"
                     /translation="MKRTYQPSKLRRARKHGFRNRMSTKNGRRVLAARRRKGRKVLAA
                     "
     gene            5330..5995
                     /locus_tag="VYN69_000212"
                     /pseudo
     CDS             5330..5995
                     /locus_tag="VYN69_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153795.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
     gene            6300..7073
                     /locus_tag="VYN69_000213"
     CDS             6300..7073
                     /locus_tag="VYN69_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEV1532569.1"
                     /translation="MIFDTHTHLNVEEFAGREAEEIALADEMGVTQMNIVGFDKPTIE
                     HALELVDEYEQLYATIGWHPTEAGTYTEEVEAYLLDKLKHSKVVALGEIGLDYHWMTA
                     PKEVQEQVFRRQIQLSKDLDLPFVVHTRDALEDTYEIIKSEGVGPRGGIMHSFSGTLE
                     WAEKFVDLGMTISFSGVVTFKKATDLQEAAKELPLDKMLVETDAPYLAPVPKRGRENK
                     TAYTRYVVDFIADLRGMTTEELAAATTANAERIFGLDSK"
     gene            7073..7633
                     /gene="rnmV"
                     /locus_tag="VYN69_000214"
     CDS             7073..7633
                     /gene="rnmV"
                     /locus_tag="VYN69_000214"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEV1532570.1"
                     /translation="MKEKISQVIVVEGRDDTVNLKRYFDVETYETRGSAINDQDIERI
                     QRLHQRHGVIVFTDPDFNGERIRRMIMTAIPTVQHAFLKRDEAVPKSKTKGRSLGIEH
                     ASYEDLKTALAQVTEQFEHESQFDISRSDLIRLGFLVGADSRKRREYLGETLRIGYSN
                     GKQLLKRLELFGVTLAEVEEAMKSYE"
     gene            7705..7848
                     /locus_tag="VYN69_000215"
     CDS             7705..7848
                     /locus_tag="VYN69_000215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1532571.1"
                     /translation="MLIGQKIKEIRIEKGISRPDFCGDEQELTVRQLSRIESGASQPS
                     CPS"
     gene            7890..8603
                     /locus_tag="VYN69_000216"
     CDS             7890..8603
                     /locus_tag="VYN69_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEV1532572.1"
                     /translation="MPDFSALPPAYLELKYQILREPIYGKEEEYDKKEACLEEIYKTY
                     FDNLPKEEQLACEVLQACLDTSRTRRPEYAELILEEHMPQIIEKEVYSTNDMLLIRLF
                     FYQMLIRKDLAKFINQIEKLMLFLLEQKKVTKLENFFIIRDTLISGMCCLEKVGVTDC
                     FNDYLSCLQEIMDKTQDYQKKPLVFMFLWKQALRVERDFSLAESFYQSSKTFAKLIGD
                     GFLVKKLTEEWQEDVKKYL"
     gene            8872..10902
                     /locus_tag="VYN69_000217"
     CDS             8872..10902
                     /locus_tag="VYN69_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1532573.1"
                     /translation="MKRLFILISMVLVSLYMVITSVDHREEILFGNYPSVDVTGMMIN
                     QPVASREKVTEALSHLAVEHNSLIARRIVEPNEAGETLFTYATYGEGELPEGLTISSK
                     ESAETSDLLGSYLIVSGSLDGVSLQTTLKELGYQGFVSNGEDPFSIVLLLTATPMVLL
                     SLAIFLLTFMSLTLIYRIKSLRQAGIRLIAGESLFGVALRPVLEDVRQLICSVLVSSI
                     LGLGILWYQGALFMATVQLVIIALLLYGLILAGISTLLSVVYLLGLQENSLVDLLKGK
                     LPLKRMMTLMMVGQLLAVLVVGSSATALLPHYREMQEMERASNKWSQYSDRYRLSFGW
                     SSAFADEEGTRKDNREWQTFTEERLANTDSFYIMSNVDNFSDGAEVDLDGNRLSDYTP
                     SGNVIYVSPRYLIEEKITVSSEFMDKMQNLSEGEFGLILPESLREQSAYYQGLFTDYL
                     QNFSSESVEVTSQKHYFPQVRLAFTETGQERFLYNDGYKTTRQYLKDPIIVVLTPQAT
                     GTRPVAGMLWGTTANSALKLDRYGDSITALKEQGLYHKVSYLVKSQLFFAKVLNDKRV
                     EFYSLLIGTILTLSTAILLFDSMNLLYFEQFRRELMIKRLAGMTIYELHGKYLLAQGG
                     VLLLGLVLSSILTRDGLISALVVALFTLNALLILVRQDKKEEAGSMAVLKGK"
     gene            10904..11545
                     /locus_tag="VYN69_000218"
     CDS             10904..11545
                     /locus_tag="VYN69_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1532574.1"
                     /translation="MIDIQGLEKKFNDRAIFSGLNLKLEKGKVYALIGKSGSGKTTLL
                     NILGKLEKIDGGRVLYQGKDLKTIPTREYFRDQMGYLFQNFGLLENQSIKENLDLGFV
                     GQKISKVERLERQVGALEKVNLGYLDLEQKIYTLSGGEAQRVALAKTILKNPPLILAD
                     EPTAALDPENSEEVMNLLVDLKDENRIIIIATHNPLVWNKADEIIDMRKLAHV"
     gene            11538..11696
                     /locus_tag="VYN69_000219"
     CDS             11538..11696
                     /locus_tag="VYN69_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532575.1"
                     /translation="MCEKIRIRRVSDYPSVRGGLEDILIMENMTNHLLLVQIRVHGYL
                     LDFASIEG"
     gene            12082..12954
                     /gene="rsmA"
                     /locus_tag="VYN69_000220"
     CDS             12082..12954
                     /gene="rsmA"
                     /locus_tag="VYN69_000220"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEV1532576.1"
                     /translation="MRIADYSVTKAVLERHGFTFKKSFGQNFLTDTNILQKIVDTAEI
                     DDQVNVIEIGPGIGALTEFLAERAAQVMAFEIDHRLVPILADTLRDFDNVTVVNEDIL
                     KVDLAQHIQNFKNPNLPIKVVANLPYYITTPILMHLIESGIPFSEFVVMMQKEVADRI
                     SAQPNTKAYGSLSIAVQYYMTAKVAFIVPRTVFVPAPNVDSAILKMVRRPEPAVAVED
                     ENFFFKVSKASFIHRRKTLWNNLTGYFGKTEEVKDKLTKALNQAGLSPSVRGEALSLA
                     EFAGLADALKGQGL"
     gene            12956..13834
                     /gene="rsgA"
                     /locus_tag="VYN69_000221"
     CDS             12956..13834
                     /gene="rsgA"
                     /locus_tag="VYN69_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEV1532577.1"
                     /translation="MQGQIIKALAGFYYVESDGQVYQTRARGNFRKKGHTPYVGDWVD
                     FSAEENSEGYILKIHERKNSLVRPPIVNIDQAVVIMSVKEPDFNSNLLDRFLVLLEHK
                     GIHPIVYISKMDLLEDRGELDFYRQTYGDIGYDFVTSKEELLSLLTGKVTVFMGQTGV
                     GKSTLLNKLVPDLNLETGEISDSLGRGRHTTRAVSFYNLNGGKIADTPGFSSLDYEVS
                     RAEDLNQAFPEIATVSRDCKFRTCTHTHEPSCAVKPAVEEGVIATFRFDNYLQFLSEI
                     ENRRETYKKVSKKIPK"
     gene            13845..14501
                     /locus_tag="VYN69_000222"
     CDS             13845..14501
                     /locus_tag="VYN69_000222"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEV1532578.1"
                     /translation="MSQYKIAPSILAADYANFEREIKRLEATGAEYAHIDIMDSHFVP
                     QISFGAGVVESLRPHSKMVFDCHLMVSNPEHHLEDFARAGADIISIHVEATPHIHGAL
                     QKIRSLGVKPSVVINPGTSVEAIKHVLHLVDQVLVMTVNPGFGGQAFLPETMDKVREL
                     VALREEKGLNFEIEVDGGIDDQTIAQAKEAGATVFVAGSYVFKGDVNERVQTLRKQLD
                     "
     gene            14464..15126
                     /locus_tag="VYN69_000223"
     CDS             14464..15126
                     /locus_tag="VYN69_000223"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000006147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEV1532579.1"
                     /translation="MSEYKLSENNWTRVAVFAGGDRGHYRTDFDAFVGVDRGSLWVLE
                     EDLPLALAVGDFDSVTEEERQVIQKRAQHFVQARPEKDDTDLELALLTIFEQNPQAQV
                     TIFGALGGRIDHMLANVFLPSNPKLAPYMHQIEIEDGQNLITYCPEGTSQLEPRSDYD
                     YLAFMPVRDSQLTILGAKYELTEENFFFKKVYASNEYIDREVSVTCPDGYVVVLHSKD
                     RR"
     gene            15128..16384
                     /locus_tag="VYN69_000224"
     CDS             15128..16384
                     /locus_tag="VYN69_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HEV1532580.1"
                     /translation="MESLLILLLIANLAGLFLIWQRQDRQEKHLSKSLEDQADHLSDQ
                     LDYRFDQARQASQLDQKDLEVAVSDRLQEVRIELHQGLTQVRQEMTDNLLQTRDKTDQ
                     RLQALQESNEQRLEQMRQTVEEKLEKTLQTRLQASFETVSKQLESVNRGLGEMQTVAR
                     DVGALNKVLSGTKTRGILGELQLGQIIEDIMTPAQYEREYATVENSSERVEYAIKLPG
                     QGDQEYVYLPIDSKFPLADYYRLEEAYETGDKDEIERCRKSLLASVKRFAKDIKSKYI
                     APPRTTNFGILFVPTEGLYSEIVRNPVFFDDLRREEQIIVAGPSTLSALLNSLSVGFK
                     TLNIQKSADHISKTLASVKTEFGKFGGILVKAQKHLQHASGNIDELLNRRTIAIERTL
                     RHIELSEGEPALDLLHFQENEEEYED"
     gene            16374..17315
                     /locus_tag="VYN69_000225"
     CDS             16374..17315
                     /locus_tag="VYN69_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM family protein"
                     /protein_id="HEV1532581.1"
                     /translation="MKISHMKKDELFEGFYLIKSADLRQTRAGKNYLAFTFQDDSGEI
                     DGKLWDAQPHNIEAFTAGKVVHMKGRREVYNNTPQVNQITLRLPQAGEPNDPADFKVK
                     SPVDVKEIRDYMSQMIFKIENPVWQRIVRNLYTKYDKEFYSYPAAKTNHHAFETGLAY
                     HTATMVRLADAISEVYPQLNKSLLYAGIMLHDLAKVIELTGPDQTEYTVRGNLLGHIA
                     LIDSEITKTVMELGIDDTKEEVVLLRHVILSHHGLLEYGSPVRPRIMEAEIIHMIDNL
                     DASMMMMSTALALVDKGEMSNKIFAMDNRSFYKPDLD"
     gene            17582..18409
                     /gene="purR"
                     /locus_tag="VYN69_000226"
     CDS             17582..18409
                     /gene="purR"
                     /locus_tag="VYN69_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004242125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEV1532582.1"
                     /translation="MKLRRSDRMVVISNYLINNPYKLTSLNTFAEKYESAKSSISEDI
                     VIIKRAFEEIEIGHIQTVTGAGGGVIFTPSISSQDAKEMVEDLRTKLSESDRILPGGY
                     IYLSDLLSTPAILKNIGRIIAKSFMDQKIDAVMTVATKGVPLANAVANVLNVSFVIVR
                     RDLKITEGSTVSVNYVSGSSGDRIEKMFLSKRSLKAGSRVLIVDDFLKGGGTVNGMIS
                     LLREFDSELAGVAVFADNAQEEREKQFDYKSLLKVTNIDVKNQAIDVEVGNIFDEDK"
     gene            18476..19726
                     /locus_tag="VYN69_000227"
     CDS             18476..19726
                     /locus_tag="VYN69_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000856498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEV1532583.1"
                     /translation="MKTPFINREELEAIVAEFPTPFHLYDEKGIREKARAVNQAFSWN
                     KGFKEYFAVKATPTPAILKILQEEGCGVDCSSYVELLMSHKLDFLGSEIMFSSNNTPD
                     KEYAYARELGATINLDAFEDIEHLERVAGIPEIISCRYNPGGVFELGTDIMDNPGEAK
                     FGMTKDQLFEVFAILKEKGAKTFGLHSFLASNTVTHLYYPELARQLFELAVEIKEKLG
                     ISLDFINLSGGIGVNYRPDQEPNDIALIGEGVRKVYEEVLTSAGLGQVKIFTELGRFM
                     LAPHGALVTRVTHKKETYRTYLGVDASAVNLMRPAMYGAYHHISNVTHPDGPAEAVDV
                     VGSLCENNDKFAVNRELPHTEIGDLLVIHDTGAHGFSMGYQYNAKLRSAEILYTEEGK
                     ARQIRRAERPEDYFATLYGFDFEE"
     gene            19852..20646
                     /gene="pflA"
                     /locus_tag="VYN69_000228"
     CDS             19852..20646
                     /gene="pflA"
                     /locus_tag="VYN69_000228"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001288284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEV1532584.1"
                     /translation="MSEETIDYGQVTGMVHSTESFGSVDGPGIRFIVFLQGCHMRCQY
                     CHNPDTWAMESNKSRERTVDDVLTEALRYRGFWGNKGGITVSGGEALLQIDFLIALFT
                     KAKEQGIHCTLDTCALPFRNKPRYLEKFDKLMAVTDLVLLDIKEINEEQHKIVTSQTN
                     KNILACAQYLSDIGKPVWIRHVLVPGLTDRDDDLIELGKFVKTLKNVDKFEILPYHTM
                     GEFKWRELGIPYSLEGVKPPTADRVKNAKQLMDTESYQDYMKRVHG"
     gene            complement(20721..21647)
                     /gene="yidC"
                     /locus_tag="VYN69_000229"
     CDS             complement(20721..21647)
                     /gene="yidC"
                     /locus_tag="VYN69_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1532585.1"
                     /translation="MKSIKRFALSAMGVAMLLVLTGCVNVDKTTGQPTGFIWNTIGAP
                     MAEAIKYFATDKGLGFGVAIIIVTIIVRLIILPLGIYQSWKATLHSEKMNALKHVLEP
                     HQTRLKEATTQEEKLEAQQALFAAQKEHGISMFGGVGCFPILLQMPFFSAIYFAAQHT
                     EGVAQASYLGIPLGSPSMILVACAGVLYYLQSLLSLHGVEDEMQREQIKKMIYMSPLM
                     IVVFSLFSPASVTLYWVVGGFMMILQQFIVNYIVRPKLRKKVREELAKNPSKASAFST
                     PSGRKDVTPEQPTAITSKKKHKNRNAGKQRSR"
     gene            complement(21725..22003)
                     /locus_tag="VYN69_000230"
     CDS             complement(21725..22003)
                     /locus_tag="VYN69_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEV1532586.1"
                     /translation="MQKVRMIAQGRVQGVGFRWGVYSLALEIGGITGRVWNNDDGTVE
                     ILAQADSSAIMAKFIQEIRKGPTPFSKVSYLDVKLSNFPPYPDFKIAN"
     gene            22037..22777
                     /locus_tag="VYN69_000231"
     CDS             22037..22777
                     /locus_tag="VYN69_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEV1532587.1"
                     /translation="MTIITSKANSVVKNTKKLHQKKYRKSAYLIEGWHLFEEAVQAGV
                     TIEKIFTLENYRDQLATFSQTVWVSEDILLDLADSQTPQGIVAVVQKEEVGQADLSQG
                     KFLFLEDVQDPGNVGTIIRTADAAGFTGVIVSDKSADIYSLKTLRSMQGSHFHLPIYR
                     MTSQALLDETKKVAIPVLATTLSKDSVDYRELPSIENFVLVMGNEGQGISPLMAESAD
                     QLVHISMKGQAESLNVAVAAGILIFHLS"
     gene            22833..23516
                     /locus_tag="VYN69_000232"
     CDS             22833..23516
                     /locus_tag="VYN69_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEV1532588.1"
                     /translation="MNHTIIHDRAGLNQFYAKVYAFVGLGIGLSALVSGLMLTVFQSQ
                     LVYLLMQGRLWLTIATFAELALVFVASSMASRNSPAALPVFLLYSVLNGFTLSFVVAF
                     YTPGTVLSAFVSSALLFFVMAAVGIFTKKDLSGIGRAMMAALIGLLIAMVVNIFLASG
                     FFDYMISVAMVLVFSGLIAWDNQKIRLAYEQSQGRVATGWVVSMALSIYLDFINLFLS
                     ILRIFGRND"
     gene            23628..24620
                     /locus_tag="VYN69_000233"
     CDS             23628..24620
                     /locus_tag="VYN69_000233"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016502595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HEV1532589.1"
                     /translation="MKKSFIHQQEEISFVKNTFTQYLKDKLEVVEVQGPILSKVGDGM
                     QDNLSGVENPVSVKVLQIPDATYEVVHSLAKWKRHTLARFGFGEGEGLFVHMKALRPD
                     EDSLDATHSVYVDQWDWEKVIPNGKRNIVYLKETVEKIYKAIRLTELAVEARYDIESI
                     LPKQITFIHTEELVERYPDLTPKERENAICKEFGAVFLIGIGGELPDGKPHDGRAPDY
                     DDWTSESENGYKGLNGDILVWNESLGGAFELSSMGIRVDEETLRRQVEITGDEDRLEL
                     EWHKSLLNGLFPLTIGGGIGQSRMAMFLLRKRHIGEVQTSVWPQEVRDTYENIL"
     gene            24685..25224
                     /gene="rsmD"
                     /locus_tag="VYN69_000234"
     CDS             24685..25224
                     /gene="rsmD"
                     /locus_tag="VYN69_000234"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEV1532590.1"
                     /translation="MKIVSGIYGGRPLKTLEGKTTRPTSDKVRGAIFNMIGPYFEVGR
                     VLDLYAGSGGLSIEAVSRGMSSAVLVERDRKAQTIVAENIQMTKEVGKFQLLKMDAER
                     ALEQVSGEFDLVFLDPPYAKEQIVADIEKMAERELFSEDVMVVCETDKAVELPEEIAC
                     LGIWKEKIYGISKVTVYVR"
     gene            25214..25702
                     /gene="coaD"
                     /locus_tag="VYN69_000235"
     CDS             25214..25702
                     /gene="coaD"
                     /locus_tag="VYN69_000235"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEV1532591.1"
                     /translation="MSDKIGLFTGSFDPMTNGHLDIIERASRLFDKLYVGIFFNPHKQ
                     GFLPLENRKRGLEKALGHLENVEVVASHDKLVVDVAKRLGATFLVRGLRNAADLQYEA
                     SFDYYNHQLSSDIETIYLHSRPEHLYISSSGVRELLKFGQDIACHVPESILEEIRNEK
                     KD"
     gene            25686..26723
                     /locus_tag="VYN69_000236"
     CDS             25686..26723
                     /locus_tag="VYN69_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEV1532592.1"
                     /translation="MKKKIRWPLYVIAALIVTFLAFVVPLPYYIEVPGGSEDIRQVLK
                     VNDTEDKEAGAYQFVTVGVQHATLAHMIYAWLTPFTDIRSAQETTGGSSDVEFMRINQ
                     FYMQTSQNMAKYQGLKTAGKDIELKYFGVYVLNVTDNSTFKGILNISDTVTAVNDQTF
                     DSSKDLIDYVSSQKLGDSVKVTYEEDGQTKSAEGKIITLENGKNGIGIGLIDRTEVIS
                     NVPISFSTAGIGGPSAGLMFSLAIYTQIAHPDLRNGRIVAGTGTIDRDGNVGDIGGID
                     KKVVASARAGAAIFFAPDNPVSEEEQKAHPDAKNNYQTALEAAKTIKTDMKIVPVKTL
                     QDAIDYLKNNP"
     gene            26781..27011
                     /locus_tag="VYN69_000237"
     CDS             26781..27011
                     /locus_tag="VYN69_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014017351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEV1532593.1"
                     /translation="MVQLLFTLSSHMFFIYVSFYLLKDLVRWEKVLKVTDENTRKVRL
                     LVAFFSIVIGYILSSFFISLYHLWQEALRGLL"
     gene            27035..28318
                     /gene="murA"
                     /locus_tag="VYN69_000238"
     CDS             27035..28318
                     /gene="murA"
                     /locus_tag="VYN69_000238"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1532594.1"
                     /translation="MDKIVVQGGDNRLVGSVTIEGAKNAVLPLLAATILASEGKTVLQ
                     NVPILSDVFIMNQVVGGLNAKVDFDEEAHLVKVDATGDITEEAPYKYVSKMRASIVVL
                     GPILARVGHAKVSMPGGCTIGSRPIDLHLKGLEAMGVKISQTAGYIEAKAERLHGAHI
                     YMDFPSVGATQNLMMAATLADGVTVIENAAREPEIVDLAILLNEMGAKVKGAGTETIT
                     ITGVEKLHGTTHNVVQDRIEAGTFMVAAAMTGGDVLIRDAVWEHNRPLIAKLLEMGVE
                     VIEEDEGIRVRSQLENLKAVHVKTLPHPGFPTDMQAQFTALMTVAKGESTMVETVFEN
                     RFQHLEEMRRMGLHSEIIRDTARIVGGQPLQGAEVLSTDLRASAALILTGLVAQGETV
                     VGKLVHLDRGYYGFHEKLAQLGAKIQRIEASDEDE"
     gene            28311..28499
                     /locus_tag="VYN69_000239"
     CDS             28311..28499
                     /locus_tag="VYN69_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1532595.1"
                     /translation="MNKKSSYVINRLLLVIIVLILGTLALGIGLMVGYGILGKGQDPW
                     AILSPAKWQELIHKFTGN"
     gene            28538..29362
                     /gene="endA"
                     /locus_tag="VYN69_000240"
     CDS             28538..29362
                     /gene="endA"
                     /locus_tag="VYN69_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-entry nuclease EndA"
                     /protein_id="HEV1532596.1"
                     /translation="MNKKTRQTLIGLLVLLLLSTGSYYIKQMQSTANSPKTKLSQKKQ
                     APEAPSQALAESVLTDAVKSQIKGSLEWNGSGAFIVNGNKTNLDAKVSSKPYADNKTK
                     TVGKETVPTVANALLSKATRQYKNRKETGNGSTSWTPPGWHQVKNLKGSYTHAVDRGH
                     LLGYALIGGLDGFDASTSNPKNIAVQTAWANQAQAEYSTGQNYYESKVRKALDQNKRV
                     RYRVTLYYASNEDLVPSASQIEAKSSDGELEFNVLVPNVQKGLQLDYRTGEVTVTQ"
     gene            29653..30984
                     /locus_tag="VYN69_000241"
     CDS             29653..30984
                     /locus_tag="VYN69_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEV1532597.1"
                     /translation="MEDPSSQNLLLQFVLLFILTVLNAFFSATEMAMVSLNRARVEQK
                     AEEGDRRYIRLLKVLENPNHFLSTIQVGITLITILSGASLTDTLGRVIASWLGNGETA
                     QAVATFLSLAFLTYISIVFGELYPKRIALNLKDALAIRTAPIIIGIGKLVSPFVWLLA
                     ASTNFLSHLTPMSFDDADEKMTRDEIAYMLTNSEETLDADEIEMLQGVFSLDELMARE
                     VMVPRTDAFMVDIQDDSQAIIQSILKQNYSRIPVYDGDKDNVIGIIHTKSLLKAGFVD
                     GFDNIVWKRILQDPLFVPETIFVDDLLKELRNTQRQMAILLDEYGGMAGLVTLEDLLE
                     EIVGEIDDETDKAAIDVHQIGEDTYIVQGTMTLNDFNNYFDVELESDDVDTIAGYYLT
                     GVGTIPTTEKLSYELVSQNKQFILTNDKVKNGRVTKVKVQITEVEIEEETE"
     gene            complement(31182..31252)
                     /locus_tag="VYN69_000242"
     tRNA            complement(31182..31252)
                     /locus_tag="VYN69_000242"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31218..31220),aa:Cys,seq:gca)
     gene            31668..35279
                     /gene="rpoB"
                     /locus_tag="VYN69_000243"
     CDS             31668..35279
                     /gene="rpoB"
                     /locus_tag="VYN69_000243"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEV1532598.1"
                     /translation="MAGHDVQYGKHRTRRSFSRIKEVLDLPNLIEIQTDSFKAFLDHG
                     LKEVFEDVLPISNFTDTMELEFVGYEIKEPKYTLEEARIHDASYSAPIFVTFRLINKE
                     TGEIKTQEVFFGDFPIMTEMGTFIINGGERIIVSQLVRSPGVYFNDKVDKNGKVGYGS
                     TVIPNRGAWLELESDSKDITYTRIDRTRKIPFTTLVRALGFSGDDEIFDIFGDSELVR
                     NTVEKDIHKNPMDSRTDEALKEIYERLRPGEPKTAESSRSLLVARFFDPRRYDLAAVG
                     RYKINKKLNVKTRLLNQTIAEPLVDPETGEILVEAGTIMTRSVIESIESHLDGDLNKI
                     VYIPNDAAVVTEPVVLQKFKVIAPTDPDRVVTIIGNANPDDKVRTVTPADILAEMSYF
                     LNLAEGLGRVDDIDHLGNRRIRAVGELLANQVRLGLSRMERNVRERMSVQDNEVLTPQ
                     QIINIRPVTAAVKEFFGSSQLSQFMDQHNPLSELSHKRRLSALGPGGLTRDRAGYEVR
                     DVHYTHYGRMCPIETPEGPNIGLINNLSSYGHLNKYGFVQTPYRKVDRETGVVTNEIV
                     WLTADEEDEYTVAQANSRLNEDGTFAEKIVMGRHQGVNQEYPANIVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPYVGTGMEYQAAHDSGAAVIAQY
                     DGKVTYADADKVEVRREDGSLDVYHIQKFRRSNSGTAYNQRTLVKVGDVVEKGDFIAD
                     GPSMENGEMALGQNPIVAYMTWEGYNFEDAVIMSERLVKDDVYTSVHLEEYESETRDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVKEGDILVGKVTPKGEKDLSAEERL
                     LHAIFGDKSREVRDTSLRVPHGADGVVRDVKIFTRVNGDELQSGVNMLVRVYIAQKRK
                     IKVGDKMAGRHGNKGVVSRIVPVEDMPYLPDGTPVDIMLNPLGVPSRMNIGQVMELHL
                     GMAARTLGIHIATPVFDGASSEDLWSTVKEAGMDSDAKTILYDGRTGEPFDNRVSVGV
                     MYMIKLHHMVDDKLHARSVGPYSTVTQQPLGGKAQFGGQRFGEMEVWALEAYGASNVL
                     QEILTYKSDDINGRLKAYEAITKGKPIPKPGVPESFRVLVKELQSLGLDMRVLDEDDQ
                     EVELRDLDEGMDEDVIHVDDLEKAREKAAQEAKAAFEAEEAEKATKAEATEEAAEQE"
     gene            35312..38989
                     /gene="rpoC"
                     /locus_tag="VYN69_000244"
     CDS             35312..38989
                     /gene="rpoC"
                     /locus_tag="VYN69_000244"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000228741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEV1532599.1"
                     /translation="MVDVNRFKSMQITLASPSKVRSWSYGEVKKPETINYRTLKPERE
                     GLFDEVIFGPTKDWECACGKYKRIRYRGIVCDRCGVEVTRTKVRRERMGHIELKAPVS
                     HIWYFKGIPSRMGLTLDMSPRALEEVIYFAAYVVIDPKDTPLEHKSIMTEREYRERLR
                     EYGYGSFVAKMGAEAIQDLLKQVDLEKEIAELKEELKTATGQKRVKAIRRLDVLDAFY
                     KSGNKPEWMILNILPVIPPDLRPMLQLDGGRFASSDLNDLYRRVINRNNRLARLLELN
                     APGIIVQNEKRMLQEAVDALIDNGRRGRPITGPGSRPLKSLSHMLKGKQGRFRQNLLG
                     KRVDFSGRSVIAVGPTLKMYQCGVPREMAIELFKPFVMREIVARDIVQNVKAAKRLVE
                     RGDERIWDILEEVIKEHPVLLNRAPTLHRLGIQAFEPVLIDGKALRLHPLVCEAYNAD
                     FDGDQMAIHVPLSEEAQAEARILMLAAEHILNPKDGKPVVTPSQDMVLGNYYLTMEEA
                     GREGEGMVFKDRDEAVMAYRNGYVHLHSRVGIATDSLNKPWTEEQRHKVLLTTVGKIL
                     FNDIMPEGLPYLQEPNNANLTEGVPAKYFLPLGGDIKEAISNLELNPPFKKKNLGNII
                     AEIFKRFRTTETSALLDRMKNLGYHHSTLAGLTVGIADIPVVDDKAEIIEESHKRVEQ
                     ITKQFRRGMITDDERYNAVTAEWRAAREKLEKRLIANQDPKNPIVMMMDSGARGNISN
                     FSQLAGMRGLMAAPNGRIMELPILSNFREGLSVLEMFFSTHGARKGMTDTALKTADSG
                     YLTRRLVDVAQDVIIREDDCGTDRGLLIRSIAEGKEMIESLEERLNGRYTKKTVKHPE
                     TGAVIIGPNELITEDKAREIVNAGVEEVTIRSVFTCNTRHGVCRHCYGINLATGDAVE
                     VGEAVGTIAAQSIGEPGTQLTMRTFHTGGVASNTDITQGLPRVQEIFEARNPKGEAVI
                     TEVKGQVTAIEEDASTRTKKVFVKGETGEGEYVVPFTARMRVEVGGQVARGAALTEGS
                     IQPKRLLAVRDVLSVETYLLGEVQKVYRSQGVEIGDKHIEVMVRQMIRKVRVMDPGDT
                     DLLMGTLMDINDFTDANKDVLIAGGVPATGRPVLMGITKASLETNSFLSAASFQETTR
                     VLTDAAIRGKKDHLLGLKENVIIGKIIPAGTGMARYRNLEPHAVNEEEYLNPPVEEEG
                     NEETTEVVVDTAVETVEETVE"
     gene            39100..39513
                     /gene="ndk"
                     /locus_tag="VYN69_000245"
     CDS             39100..39513
                     /gene="ndk"
                     /locus_tag="VYN69_000245"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000438285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEV1532600.1"
                     /translation="MEQTFFIIKPDGVKRGLVGEVLKRIEQRGFTIEKLEFRSQVSEE
                     LIDQHYQDLVGQSFYPPIREFMTSGPVLVGVISGPKVIETWRTMMGATRPEEALPGTI
                     RGDFAKAAGENEIIQNVVHGSDSEESAKREIALWF"
     gene            complement(40442..41074)
                     /locus_tag="VYN69_000246"
     CDS             complement(40442..41074)
                     /locus_tag="VYN69_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1532601.1"
                     /translation="MIELKQVSKSFGERELFSNLSMTFEAGKVYALIGSSGSGKTTLM
                     NMIGKLEPYDGTIFYRGKDLANYKSSDFFRHELGYLFQNFGLIENQSIEENLKLGLIG
                     QKLSRSEQRLRQKQALEQVGLAYLDLDKRIFELSGGESQRVALAKIILKNPPFILADE
                     PTASIDPATSQLIMEILLSLRDDNRLIIIATHNPAIWKMADEVFTMDHLK"
     gene            complement(41076..43091)
                     /locus_tag="VYN69_000247"
     CDS             complement(41076..43091)
                     /locus_tag="VYN69_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1532602.1"
                     /translation="MKKLFILISNLLASLFFVWVFTIWTDTYVSYYYSNVVVHDSSPE
                     TTFQHVATRLEKLAEETDSFIAIQHQDPNSEGTTVFSYTTFGDGKLPDGLQEKKLEDA
                     QSSSVETNYFVFDGHLDIHLLREELSQLGLTNMNLTIPSKLSTLMAIFSNGFQLISLL
                     IFILTFVALTLISQISQLRSSGIRLISGEKRWSIFLRPVGEDLKGIAVGFSLAGVLAI
                     LMQKILSLPTQSLMTIGAGLLSYNLILLSISLFFAQLFAVGIKKIHLMQIIKGQVPVR
                     GIISLILIGQLLAIIIVTLGIGSSLKYSQAWQQHRIGQEAWSQERQLITLSISREGTS
                     PGFDEQAQRKLRTWYQLMDLAVSEQKAFLSRHQLIDRTLQNGMASSKNLITSTEWHDY
                     NPNGNVLIVTPQYLERQNIPVDTTIEQKMNHLDVGEFVLLLPEHLRSEEEHYKSVFED
                     DLTSRMSSQDERQQMTATVGYLESGQDRFVYNTTPISYQQFLKDPIIIVITPQSTGPQ
                     SILFWIDAVQNYVLFNQLSDAQELIQRQGIENWVSEMQTGYHNYITLLDNIQRERWVM
                     LAGAVLGIATSILLFNTMNRLYFEEFRRAIFIKRIAGLRFLEIHRTYLFAQLGVFLLG
                     FVASVFLQVEIGVAFLVLLLFTGLSLLQLHVQMQKENKMSILVLKGG"
     gene            43595..44458
                     /locus_tag="VYN69_000248"
     CDS             43595..44458
                     /locus_tag="VYN69_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1532603.1"
                     /translation="MNTLAEKFRLKRKELRLSQQTLAEGICEQSQISKIERGHFIPSA
                     DLLFKLSQRLEVPLDYFFNEQIEIKSNLSNFKQLSARLLDDRNYDDLEYIYRIEIERS
                     TFLTLEDRTYLEWIKAIIDFYQYDSKCEAISSLENILLKVSSNTLIYLKALNTLSNFY
                     SLVGREQEYEANYSHLIELYQTKNLDHQEFLFGYIRVRYNYAHYLVSKEKYNEAIQEA
                     LETIELCKQRQTSYQLAPLLILVGNAGAKFLDREQVKNYYIEARELCKIYNNPLMLMK
                     IENYLKELDTV"
     gene            44841..45461
                     /locus_tag="VYN69_000249"
     CDS             44841..45461
                     /locus_tag="VYN69_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000839915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532604.1"
                     /translation="MKSMRILFLLALIQISLSSCFLWKECILSFKQSTAFFIGSMVFV
                     SGICAGVNYLYTRKQEVHSVLASKKSVKLFYSMLLLINLLGAVLVLSDNLFIKNTLQQ
                     ELVDFLLPSFFFLFGLDLLIFLPLKKYVRDFLAMLDRKKTVLVTILATLLFLRNPMTI
                     VSLLIYIGLGLFFAAYLVPNSVKKEVSFYGHIFRDLVLVIVTLIFF"
     gene            45548..45991
                     /gene="tsaE"
                     /locus_tag="VYN69_000250"
     CDS             45548..45991
                     /gene="tsaE"
                     /locus_tag="VYN69_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEV1532605.1"
                     /translation="MYTKNEEELQALGERLGHLLAKNDVLILTGELGAGKTTFTKGLA
                     KGLQISQMIKSPTYTIVREYEGRLPLYHLDVYRIEGDADSIDLDEFIFGGGVTVIEWG
                     NLLGDALPDAYLELEILKEADGRRLNFQAKGLRAEKLLEELQYGV"
     gene            45981..46499
                     /locus_tag="VYN69_000251"
     CDS             45981..46499
                     /locus_tag="VYN69_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1532606.1"
                     /translation="MEYELLIREAEPKDAAELVTFLNRVSLETDFTSLDGDGILLTSE
                     EMEIFLNKQASSDNQITLLAFLNGKIAGIVNITADQRKRVRHIGDLFIVIGKRYWNNG
                     LGSLLLEEAIEWAQASGILRRLQLTVQTRNQAAVHLYQKHGFVIEGSQERGAYIEEGK
                     FIDVYLMGKLIG"
     gene            46507..47523
                     /locus_tag="VYN69_000252"
     CDS             46507..47523
                     /locus_tag="VYN69_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEV1532607.1"
                     /translation="MVKKIIGMVLALLSVTVVGVGVFAYTIYQQGTETLAKTYKKIGE
                     ETKVIEATEPLTILLMGVDTGNAERTETWVGRSDSMILMTVNPKTKKTTMMSLERDIL
                     TRIESGNGQAHEAKLNSAYADGGAELAIETIQKMMNIHIDRYVMVNMRGLQKLVDAVG
                     GITVNNILGFPISISDQEEFNTISIGVGEQHIGGEEALVYARMRYQDPEGDYGRQKRQ
                     REVIQKVMEKALSLNSVGHYQEILKALSDNMQTNIDLSAKSIPNLLGYKDSFKTIETQ
                     QLQGEGEILQGVSYQIVSRAHMLEMQNILRRSLGQEEVTQLETNAVLFEDLFGRAPVG
                     DEDN"
     gene            47608..48864
                     /locus_tag="VYN69_000253"
     CDS             47608..48864
                     /locus_tag="VYN69_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEV1532608.1"
                     /translation="MKAEIIAVGTEILTGQIVNTNAQFLSEKLAEIGVDVYFQTAVGD
                     NEVRLLSLLEIASQRSSLVILTGGLGPTEDDLTKQTLAKFLGKALVFDPQAQEKLDIF
                     FALRPDYARTPNNERQAQIVEGAIPLPNETGLAVGGKLEVDGVTYVVLPGPPSELKPM
                     VLNQLLPKLMTGSKLYSRVLRFFGIGESQLVTILADLIDNQIDPTLAPYAKTGEVTLR
                     LSTKASSQEEANQALDILENQILDCQTFEGISLRDFCYGYGEETSLASIVVEELKRQG
                     KTIAAAESLTAGLFQATVANFSGVSSIFKGGFVTYSLEEKSRMLDIPAKNLEEHGVVS
                     EFTAQKMAEQARSKTQSDFGISLTGVAGPDSLEGHPVGTVFIGLAQDQGTEVIKVNIG
                     GRSRADVRHIAVMHAFNLVRKALLSD"
     gene            48919..50085
                     /gene="recA"
                     /locus_tag="VYN69_000254"
     CDS             48919..50085
                     /gene="recA"
                     /locus_tag="VYN69_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEV1532609.1"
                     /translation="MAKKPKKLEEISKKFGAEREKALNDALKLIEKDFGKGSIMRLGE
                     RAEQKVQVMSSGSLALDIALGSGGYPKGRIIEIYGPESSGKTTVALHAVAQAQKEGGI
                     AAFIDAEHALDPAYAAALGVNIDELLLSQPDSGEQGLEIAGKLIDSGAVDLVVVDSVA
                     ALVPRAEIDGDIGDSHVGLQARMMSQAMRKLGASINKTKTIAIFINQLREKVGVMFGN
                     PETTPGGRALKFYASVRLDVRGNTQIKGTGDQKETNVGKETKIKVVKNKVAPPFKEAV
                     VEIMYGEGISKTGELLKIASDLDIIKKAGAWYSYKDEKIGQGSENAKKYLAEHPEIFD
                     EIDKQVRSKFGLIDGEEVSEQDTENKKDEPKKEEAVNEEVTLDLGDELEIEIEE"
     gene            50392..51762
                     /locus_tag="VYN69_000255"
     CDS             50392..51762
                     /locus_tag="VYN69_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1532610.1"
                     /translation="MNKKRTVDLIHGPILPSLLSFTFPILLSNIFQQLYNTADVLIVG
                     RFLGQESLAAVGATTAIFDLIVGFTLGVGNGMGIVIARYYGARNFTKIKEAVAATWIL
                     GALLSILVMLLGFLGLYPLLQYLDTPAEILPQSYQYISMIVTCVGVSFAYNLFAGLLR
                     SIGDSLAALGFLIFSALVNVVLDLYFITQLHLGVQSAGLATIISQGLSAVLCFYYIRK
                     SVPELLPQFKHFKWDKSLYADLLEQGLAMGLMSSIVSIGSVILQFSVNTFGAVIISAQ
                     TAARRIMTFALLPMTAISASMTTFASQNLGAKRPDRIVQGLRIGSRLSISWAVFVCIF
                     LFFASPALVSFLASSTDGYLIENGSLYLQISSAFYPILSLLLIYRNCLQGLGQKILPL
                     VSSFIELIGKIVFVVLIIPWAGYKGVILCEPLIWVAMTVQLYFSLFRHPLIKEGKAIL
                     ATKVQS"
     gene            52138..53094
                     /gene="lytA"
                     /locus_tag="VYN69_000256"
     CDS             52138..53094
                     /gene="lytA"
                     /locus_tag="VYN69_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase LytA"
                     /protein_id="HEV1532611.1"
                     /translation="MEINVSKLRTDLPQVGVQPYRQVHAHSTGNPHSTVQNEADYHWR
                     KDPELGFFSHIVGNGCIMQVGPVDNGAWDVGGGWNAETYAAVELIESHSTKEEFMTDY
                     RLYIELLRNLADEAGLPKTLDTGSLAGIKTHEYCTNNQPNNHSDHVDPYPYLAKWGIS
                     REQFKHDIENGLTIETGWQKNDTGYWYVHSDGSYPKDKFEKINGTWYYFDSSGYMLAD
                     RWRKHTDGNWYWFDNSGEMATGWKKIADKWYYFNEEGAMKTGWVKYKDTWYYLDAKEG
                     AMVSNAFIQSADGTGWYYLKPDGTLADKPEFTVEPDGLITVK"
     gene            53459..53620
                     /locus_tag="VYN69_000257"
     CDS             53459..53620
                     /locus_tag="VYN69_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1532612.1"
                     /translation="MNMINVCDVGKRIKELRISSNLTQDKIAEYLSLNQSMIAKMEKG
                     ERNITNGFK"
     gene            53607..53813
                     /locus_tag="VYN69_000258"
     CDS             53607..53813
                     /locus_tag="VYN69_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532613.1"
                     /translation="MDLSNKASNLRKKLGADGESPIDIFKLVQKIENLTLVFYGLGKN
                     ISGVCYKGTQFSLIAVNSDMPLGR"
     gene            53859..54218
                     /locus_tag="VYN69_000259"
     CDS             53859..54218
                     /locus_tag="VYN69_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532614.1"
                     /translation="MKKSSVSLILIGEGDETERKADQFASYFLIFPSSLYRMVEEIRE
                     NANRTHLEVEDIIKLGQFYGISHKAMLYRLRNDGYLDAEEIKNMDISVIETASRLGYD
                     TSLYRPLSESKKEMALG"
     gene            54347..54772
                     /locus_tag="VYN69_000260"
     CDS             54347..54772
                     /locus_tag="VYN69_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000204059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532615.1"
                     /translation="MTSRVFIDADCISVFLWVGTEHLLEKLYLGKIVIPQEVYDEINI
                     PTIPHLKSRIDQLVAKGSAEIVSIDIGTEEYALYRDLTRNHDSNKIIGKGEGASISLA
                     KKHNGILGSNNLRDVKSYVEEFSLEYMTTGDILIEAFKA"
     gene            55180..55974
                     /locus_tag="VYN69_000261"
     CDS             55180..55974
                     /locus_tag="VYN69_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001101215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532616.1"
                     /translation="MNVKKIMSIFQSFYVDVSIEELTLTLPISFVKRFEYTQMTFHKE
                     SFLLIKEKRRGSLSSFVTQARTMGEKANMDVVLVFSKLSDSEKKQLLQARVPFVDFKG
                     NLFFPPLGLVLNANDTEVPKELTPSEQLTWIAFLLTKGQKVVDVDLLSQVTGLPNSTI
                     YRCLRTFKALYWLNKQNKLYTYTVSKKELFLKSVSCLFNPIKKRILLPDGDIKQIKSV
                     SNLLYGGAYALSHSTFLAETDENISYVIWQRKFNQLSLPLSQHVLK"
     gene            55978..56175
                     /locus_tag="VYN69_000262"
     CDS             55978..56175
                     /locus_tag="VYN69_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000890145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532617.1"
                     /translation="MLEIWKYRPFVSEFWNDFKNNHDKQFVDPISLYLTLKDDDDPRI
                     EEESEALENMILQYLGEDDAS"
     gene            56165..>56627
                     /locus_tag="VYN69_000263"
     CDS             56165..>56627
                     /locus_tag="VYN69_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532618.1"
                     /translation="MPANTKVIFQEMFADFQNYYVLIGGTATSIVLDSQGFKSRTTKD
                     YDMVIIDEVKNKEFYTTLNHFLELGEYQGSQKDEKAQLFRFTTTNPEFPSMIELFSIL
                     PEYPLKKDGREIPLHFDQDASLSALLLDEDYYNILVHEKETIQGYSVLSNCG"
BASE COUNT        16902 a        10542 c        12792 g        16391 t
ORIGIN      
        1 cagtaaccat acatacggta aggcgacgtg acgtggtttg cagagatttt cgaagagtat
       61 tagccttcca tatagtcttt caaagcctgt cctgttagtc cggcattgag ggcgatgagg
      121 agtttcaagc gagctttttg ggcgttgagt tctttaacaa agaaaacgcc tgctttttgc
      181 aactgtacgc ccccaccctg gtaggcataa acaggctcgg caataccgtt aaagcatcgt
      241 gataccagag cgactggaat tcctttttgc agaaggcttt ctaatttttg agccgtttct
      301 ttgggaatat taccagctcc gaaggcttgg ataatcaaac cgtccaagtg ttctaaatcc
      361 agcatatcaa tcagctcatc tgtcatacca gcataagccg agatgatagg gactaaacct
      421 tgtatgtgat caaggtcaaa gcgaacacga ggttcagctg ttttgaagta gaggatttcc
      481 tgtttcatga tgagaccaag ggggccatgt gttggagtct ggaaggtgct gacattagtc
      541 gtatgtgttt tggtgacata cttggcagcg tggatttcat cgttcataac gaccaaaact
      601 cctttgtcag cagccctgtc atcgctggcc actcgtaaag cacttaggta attataaaca
      661 ccatcactac cgagttcatt ggagctacgc atggctcctg ttagaacgat aggcatatgg
      721 ggaacttcca tggtatcaag gaaataggct gtttcctcta aagtatcggt tccgtgtgtg
      781 atcaccactc catcgtagtt atctgcttcc tctttaattt tctggtagag gaccagcata
      841 tgtttgggtt tgatatgggg acttggaagg ttaaaaaagt ccaaggcgtg gacttggatt
      901 ccttcaagtg ggttggacac atggttcatg ggattatctg aactcgtcac aacagcgcca
      961 gaagcatcgg cctgcatgga aatagttcca cccgtatgta aaacaaggat tttcttaggc
     1021 atgatttact cactctttct gaaatgtggt ataatcattg tatcacaaaa ggggagattg
     1081 ggataggatg gaagtcaaag ctgttttttt tgatatcgat ggaaccttgg tcaacaatcg
     1141 caagagtgtt ttgaaatcca ctaaggatgc gattaagatt gtcaaagaac aaggggtact
     1201 agttggcgta gcgacagggc gaggaccttt ttttgttaag gaattgatgg acgatttgga
     1261 tctggatttt gcggtaacct ataatggcca gtatatcttt agtaaagaca gagtcttgtt
     1321 cacgagccct atttccaagt tacatttgcg ccatctaatt agctatgcta aaaaagaggg
     1381 cacggagatt gccttaggga ccaaggatgc catgttgggt tctaaaatca tgtcctttgg
     1441 tctgggttct ttttcccaac gaattagtcg cttcgttccc tctgttttaa ctcggacggt
     1501 gagtcagtct tttaatcgca tggtcagcaa ggttgttccc caaaaggaag aggacctgct
     1561 tcacctgatg aatcagccta tctaccaagt tttgatgctg atgacaccag aagaatctga
     1621 gaaggcggca gctgattttg aagacttgaa attgacacgt agcaatcctt ttgcatcgga
     1681 tgtcatcaat cagggaaatt ctaaattaga aggcattcgc cgagttggga aagaatatgg
     1741 ctttgacctc aatcaagtca tggcttttgg tgactcagat aatgaccttg aaatgctggc
     1801 tggcgtcggt atgtcggtcg ctatgggaaa tggtagtagc agtgtcaagg aagctgccaa
     1861 gcacattacc actagcaatc aacaagatgg gatccacaag gccttggaac attttggtgt
     1921 tttgtcttca gaaaaagtct ttgtcagccg tgactatcat ttcaataagg tcaagacctt
     1981 ccaccacatg atggatgaac gaacccaaga agaacctcga gcttgggatt tagagggtgc
     2041 aacccatagg gctggtttta aaatagaaga attggtggag tttgttcgag cagccagtcc
     2101 ttctgaagaa gattttggtc aagctgtatc gcaacttcat caggcccttg ataaggcagc
     2161 agataaagta gccaagaaga cacctgctca gcaagatttg atagggcaag tggatgcctt
     2221 gattgacacg ctttacttca cctacggcag ttttgtcttg atgggggtgg acccagaacg
     2281 tatttttgat attgtccatc aggccaatat ggggaaaatt tttccagatg gcaaggctca
     2341 ttttgatcca gtgacccata aaatcttaaa accagataat tgggaagaaa aatatgctcc
     2401 agaacctgct attaaaaagg aactgcagcg ccagctcaag gcttacgaac gccataaaga
     2461 gagaaataaa tcgtaaacaa aaaggagcta ggggctcctt ttctttgtga ttataaggtt
     2521 ttttcttgtt ctctcacaac cagcaaatcg accttagcat ggcggagtat gtattcagat
     2581 gaagagccga ctaagaggcg ttcaaaggcg ttgagaccag ttgcgccaac gaggatgagg
     2641 tccacttcct cggcatctgg aatagtacgt gctagcaggg tctttggatt tcccatttca
     2701 atgacgatat gaacatctgc taccccagca tctttagcac gtttttcgta ctctttcatc
     2761 agactttcag cgtcgacttg gagttcttcg taaacttcag catcaaaggt ggatacgctt
     2821 tggagagcgc gtgtgtcaat gacatgtacg atggtgagtt tagcgtcgtt tcgtagagca
     2881 gaatgaactc ccttgacaaa agccaagtcc gcttccttag aaccatcgat tgcgaccatg
     2941 atattttggt aacgttgtgc cataatgaaa cctcctgaaa ttttcttact ataattgtaa
     3001 accttttcac tatagaagta aagcaaaaaa agtatttttc aaaagaatat tagggcttaa
     3061 aatctatata aatatatgat aaaataaaag aaaggatagc aggaaagaag tgaaggaagg
     3121 gagaatgatg aaaaactctt ttcaaaaatc aaatttcctt tattatggaa ttatcctagt
     3181 ttcagtcttg gtagaagtga tcatgatctg gcaattagag agtctggtac cgcttcttta
     3241 tccaagtttt attggatttt tagtctttca tgtactctac catctaatct tatttctagt
     3301 tgcaaaacga agtgggcgtt gggattactt gatgatatgg gggcttttcc tcatgtttaa
     3361 ttttctttat gactcctttc taggcttgct ttttctaggt ttatcttttg gtatgtgatg
     3421 tgtctttgca aaatacctgc tatctctacg ttcttaacct cgcctttagc aaggtttttc
     3481 ttctatacga aactagtaac acatttcagt aaacttgtcg cattctcttt ctagtggtat
     3541 aatgttacta tcctgatgaa ttgaggaaac aagatgaaag aatataacaa gtctagtaag
     3601 ttagagcatg ttgcttatga tatccgtggt cctgtattgg aagaagccat gcggatgcga
     3661 gcgaatggag aaaagatttt acgtctgaat acaggaaatc cagcagaatt tggctttaca
     3721 gcgccagacg aggtcattca tgacttgatt atgaatgcgc gtgatagtga aggctattct
     3781 gactccaaag ggattttctc agcccgtaag gccatcatgc agtattgcca gctgaagaaa
     3841 ttcccaaacg tagatattga tgatatttac cttggaaatg gtgtcagtga gttgattgtc
     3901 atgtccatgc aggggctttt ggacaatggg gatgaggtct tagtgccaat gccagactat
     3961 cctctatgga cggcggctgt cagcctagct ggaggaaatg ccgttcacta tatctgtgat
     4021 gaagctgtgg aatggtaccc agatattgac gatatcaagt caaaaatcac ttccaatacc
     4081 aaggcaattg tccttatcaa tccaaataac ccaactggag ccctttatcc taaggaactc
     4141 ttgctggaga ttattgagat tgcccgtcaa aacgatttga ttatctttgc ggatgaaatc
     4201 tatgaccgca tggtaatgga cggacatgtg catacgcctg tggcgagctt ggcaccagat
     4261 gtcttctgtg tcagcatgaa tggtctgtca aaatcccacc gcatagcagg tttccgtgtg
     4321 ggttggatgg tcttgtctgg ccctaagact catgttaagg gctatatcga agggctcaat
     4381 atgctgtcca atatgcgcct ttgctctaac gttttggccc aacaagtcgt acaaacttcc
     4441 ttggggggtc accaatcagt cgatgaattg cttcttcctg gtggacgaat ctacgagcaa
     4501 agaaatttca tctataatgc cattcaagat attccaggtt tgtctgccgt taaacccaag
     4561 gcggggctct atatcttccc aaaaatcgac cgcaatatgt accgtatcga tgatgatgag
     4621 cagtttgtcc ttgatttctt gaagcaggaa aaggttctct tggttcatgg tcgaggcttt
     4681 aactggcagg aaccagacca cttccgtatc gtttaccttc ctcgtgttga tgagttagcc
     4741 caaatccaag aaaagatgac tcgtttcttg aaacagtatc gtagataggg cttgcattcg
     4801 aaaaagctgg aaacatttgc ctagggctat tgacaaaagt gaaagaatca gatagaatag
     4861 taaagtatgt ttagtaactg atcactttat agccggctaa acgaatacaa aatctatgag
     4921 gaggtattca tcgtgaaacg tacttatcaa ccaagtaaac ttcgtcgtgc gcgcaaacac
     4981 ggattccgta accgtatgtc aactaaaaat ggtcgtcgcg tattggcagc tcgtcgtcgt
     5041 aaaggacgca aagttttggc tgcataatcc aaacgaattc tatcaaaaat cagtaggaac
     5101 tcgagtctac tgatttttat ttttgtaaaa aagttcagta gatgcaaatg gattcggaag
     5161 cgatgttata gtagattgaa actagaatag tacacctctg tttctaaaac attgttagaa
     5221 atcgatttga ctgtcctgat cgatttgtcc tgttattatt ttattttact ataaagttga
     5281 agtagctgga gatggcacag cgacaatcac attcccagat ggttcagtag tgacgattct
     5341 aggaaaagat acagttcaac aatctgcgaa aggtgaatct gtaactcaag aagctacacc
     5401 agagtataag ctagaaaata caccaggtgg agataaggga ggcaatactg gaagctcaga
     5461 tgctaatgcg aatgaaggcg gtggtagcca ggcgggtgga tcagctcaca caggttcaca
     5521 aaactcagct caatcacaag cttctaagca attagctact gaaaaagaat cagctaaaaa
     5581 tgccattgaa aaagcagcca agaacaagca ggatgaaatc aaaggcgcac cgctttctga
     5641 taaagaaaaa gcagaacttt tagcaagagt ggaagcagaa aaacaagcag ctctcaaaga
     5701 gattgaaaat gcgaaaacta tggaagatgt gaaggaagca gaaacgattg gagtgcaagc
     5761 cattgccatg gttacagttc ctaagagacc agtggctcct aatgctgctc ctaagacaac
     5821 aagtgcaccg caagcaactg caggaacaat gcaagatgtt acctaccagt cacctgctgg
     5881 caaacaatta cctaacacag gttaagcatc aagtgcagca cttgctagtc ttggtctagt
     5941 ggtggcaaca agtggttttg ctttgctagg aagaaagact agacgtagaa aatagaacag
     6001 ctagaaaatt ctattctcta cttaaagtta gattataaga gggattttga gaagtcatca
     6061 atcctagtga tgggtgagaa aagtgagaac ccaagataat cacatacttt agctgaatag
     6121 gaatattcta tcaatgtagc caatctcttc tgtctctaac tgtggaatag gagatgggca
     6181 atatcggata gaaaagatag cagaatagct ccctattgaa gagaggaggg gaaaccgaaa
     6241 aattaggtgc ccctcctctt ttttggtata atagaagata gaaaacgagg ttagaagaga
     6301 tgatttttga tacacataca cacttgaatg tagaagaatt tgcaggtcgt gaggcagaag
     6361 aaattgcctt ggctgatgag atgggtgtga cacagatgaa tattgttggt tttgataaac
     6421 cgacgattga gcatgccttg gagttggtag atgagtatga gcagctctat gcgactattg
     6481 gttggcatcc tacagaagct ggtacttata cagaggaagt tgaggcttac ttgttggata
     6541 agttaaaaca ttccaaggtt gtggctttag gtgaaattgg cttagactac cattggatga
     6601 cagcgcccaa agaggtgcag gagcaggttt ttcgccgtca gattcagcta tctaaggact
     6661 tggatttgcc ttttgttgtc catacccgtg atgcgctgga agatacctat gagattatca
     6721 agagtgaggg cgttggtcct cgtggtggta tcatgcattc attttcaggg acgcttgagt
     6781 gggcagagaa gtttgtggat cttggtatga ccatttcctt ctcaggagtg gtgactttca
     6841 agaaggcaac tgacctccaa gaagcagcta aagagttacc tttggacaag atgttggtgg
     6901 aaacagatgc gccttactta gcacctgtac ccaagcgtgg ccgtgaaaat aaaacagcct
     6961 atactcgcta tgtggtggac tttatcgctg acttgcgtgg tatgacgaca gaagagctag
     7021 cggcggcaac gactgcaaat gcagaacgca tttttggatt ggacagcaag taatgaaaga
     7081 gaaaatttcc caagttatcg tggttgaagg tcgcgatgat acggtcaatc tcaagcgtta
     7141 tttcgatgtg gagacctatg agactcgagg ttctgccatc aatgaccagg acatagagcg
     7201 gattcagcgt ctacaccaac gtcatggagt cattgtcttt acagacccag attttaatgg
     7261 ggagcggatt cggcgcatga ttatgacggc cattccaaca gttcagcatg cctttctcaa
     7321 gcgagatgaa gctgttccca agtccaagac caaggggcgt tctctgggaa ttgagcatgc
     7381 cagctatgaa gacctgaaaa cggctctagc tcaggtgaca gaacaatttg aacatgagag
     7441 tcagtttgac attagtcgta gcgacttgat tcgccttggt tttctagtag gggcagacag
     7501 ccgtaagcgt agggaatatc tcggagagac tctccgaatc ggctattcta acggcaagca
     7561 actcctcaaa cgcctagagt tgtttggggt tactttggca gaagtggaag aagctatgaa
     7621 atcttatgag taggaaagat gtgaccgtta caatttttta agtttcacag tatttttcga
     7681 agcaggtaga agaggaggcg tctgatgtta attggtcaaa aaattaaaga gattcggata
     7741 gaaaaaggaa ttagtcgtcc agatttttgt ggagatgagc aagaactgac agttcgtcaa
     7801 ctgtcgcgaa ttgaaagtgg agcttcgcaa ccgagttgcc caagttagac tatattgctc
     7861 accggctagg agttccagtt tatagcctta tgccggattt ttcagctctt cctcctgctt
     7921 atttagaatt gaaataccag attttacgtg aaccaatcta tggtaaagaa gaggagtatg
     7981 ataagaaaga agcgtgtttg gaagagattt ataaaacata ctttgataat cttcctaaag
     8041 aagaacaatt agcatgtgaa gtattgcagg cgtgtttgga tacttctaga actagaaggc
     8101 ctgaatatgc agagttaata cttgaggaac atatgcctca gatcatagaa aaagaagttt
     8161 attcaacaaa tgatatgttg ttgattcgtt tgttttttta tcaaatgctc attagaaaag
     8221 atcttgccaa atttataaat caaatcgaaa agctaatgct ctttcttttg gaacagaaga
     8281 aggtaactaa attagaaaac ttctttataa ttagagatac tcttatttca ggaatgtgtt
     8341 gtcttgaaaa ggtaggagta actgattgtt ttaatgatta tctatcgtgt ttacaagaaa
     8401 ttatggataa aactcaagat tatcaaaaga aacctcttgt atttatgttt ttgtggaagc
     8461 aagcattaag agtagaaaga gattttagtt tagctgaatc attttatcag tcttctaaaa
     8521 catttgcgaa gctaattgga gatggatttc tagtaaagaa attgacagag gaatggcaag
     8581 aggatgtcaa aaaatattta taaacatagt gaatcagtga caaaaatgtc cttgtcctcg
     8641 tatcaaaaca gttctaaagt tcgtctttag aactgttttt ttagatataa gctaaaaata
     8701 atatgaaata gttagatttt aaggacattg atgtccttga tagttcttga aaaaacggat
     8761 ataatatatt tgaagatgtt gctagcagaa agaacttaat aagcttctta acatcgcagt
     8821 gactgtggtc accttctcac taagtgacca agtaactttt tggaggaaat gatgaaacgt
     8881 ttatttattt tgatttcaat ggtattggta tcgctttata tggtgataac ttccgttgac
     8941 catcgagaag agattttatt tggtaactat ccttctgttg atgtgacagg aatgatgata
     9001 aatcaaccag tagctagtcg cgaaaaggtg acagaggctt tgagtcactt ggcggtagag
     9061 cataatagtc tcatcgcccg tcgaatcgtt gagccaaatg aagctggaga aacactcttt
     9121 acctatgcca cttatggtga gggagagctt ccagaaggtc tgaccatttc ctccaaggag
     9181 agtgcagaaa cgagtgattt attagggtct tacttgattg tgtcaggaag tttggatgga
     9241 gtgagcttac agaccacctt gaaagaactt ggctatcaag gctttgtttc gaatggagaa
     9301 gatccatttt cgatagtctt actattgacg gccaccccta tggtgctact gagtttagct
     9361 atttttctgc tgacctttat gagtctgacc ctgatttatc ggatcaaatc ccttcgtcag
     9421 gcagggattc gcttaatagc tggtgagagc ttgtttggag ttgctctcag accagtgtta
     9481 gaagatgtga gacagcttat ctgctcagtg ctggtatcca gtattttggg attggggatt
     9541 ctctggtatc aaggtgcctt gtttatggca acggtgcaac tggtcatcat tgctcttcta
     9601 ctttatggat tgatcttggc agggatttct accttactaa gtgtcgtcta tctacttggt
     9661 ttacaggaaa atagtctggt ggatctattg aaagggaaac tccctctcaa acgtatgatg
     9721 acattgatga tggtggggca actcttagct gtattggtgg tcggatcgag tgcgacagct
     9781 ctcctacccc actaccgtga aatgcaggaa atggagagag ctagcaataa atggagccag
     9841 tactcagacc gttaccgtct atcctttggt tggtctagtg catttgccga tgaagaagga
     9901 acgcgtaagg ataatcgtga gtggcagaca tttactgaag aacggttagc caatacagac
     9961 tctttttata ttatgagcaa tgttgacaat ttctcagatg gagcagaagt ggacctagat
    10021 ggtaatcgtc tcagtgacta cacaccgtca gggaatgtta tctatgtctc accacgctat
    10081 ctgatagaag aaaagattac cgtttcttca gagtttatgg acaagatgca aaacttgtct
    10141 gagggagagt ttgggctgat cttgcctgag agcttgcgag agcagtctgc ctactaccaa
    10201 ggattgttta cagattacct gcaaaacttt tcatctgaaa gtgtagaagt gacgagtcag
    10261 aaacactact tcccacaggt aaggctagct tttacagaaa caggacagga acgtttcctc
    10321 tataatgatg ggtacaagac aacacgccag tacctaaaag atccgattat tgtagttcta
    10381 acgccgcaag cgactggaac aagacctgtt gcagggatgt tgtggggaac tacggctaat
    10441 agtgccttga aactagatcg atatggagac agcatcacag ctctaaaaga gcaaggtctg
    10501 tatcacaagg tttcttactt ggtaaaaagc cagctatttt ttgccaaggt actaaatgac
    10561 aaacgggtgg agttttactc tctccttatt gggacgattt tgaccctgtc tacggctatc
    10621 ttgttatttg attccatgaa tcttctctat tttgagcagt tcagacggga gcttatgatt
    10681 aaacgtcttg ctggtatgac aatctatgag cttcatggca agtatttact ggcgcaagga
    10741 ggagttctct tgcttggcct agtcctatct agtattttga caagagatgg tttgattagc
    10801 gctctagttg tagctttgtt tacgcttaac gccctcttga ttttagtaag gcaggacaaa
    10861 aaagaagaag ctggtagcat ggcagtattg aaaggaaaat aagatgattg atattcaagg
    10921 attggaaaag aaatttaatg accgcgcgat tttctctggt ttgaatctca agctggagaa
    10981 gggcaaggtt tatgccttaa tcggaaagag tggaagcgga aagacgacgc tgctgaatat
    11041 cttgggaaag ctagaaaaga tagatggtgg aagggttctc tatcagggga aagatttaaa
    11101 aaccattccc actcgtgagt attttcgaga ccagatgggc tatctctttc aaaatttcgg
    11161 cctcttagaa aaccaatcaa tcaaagaaaa tttggatttg ggttttgttg gtcagaaaat
    11221 ctcaaaagta gaacgtttgg aaaggcaagt gggggcttta gaaaaagtta atctagggta
    11281 tttggattta gaacaaaaaa tctatacttt atctggggga gaggcccaac gagttgccct
    11341 tgctaagact attttgaaaa atccaccctt gattttggca gatgaaccaa cagcagctct
    11401 tgatcctgaa aattcagagg aggttatgaa tctcttggtg gatttgaaag atgaaaatcg
    11461 aattatcatc attgcgaccc ataatcccct agtctggaat aaggctgatg aaatcattga
    11521 tatgaggaaa cttgctcatg tgtgaaaaaa tccgtattcg cagggtatct gattatccta
    11581 gtgtcagagg tggtttagaa gatatcctca tcatggaaaa tatgaccaat catctccttt
    11641 tggttcaaat ccgagtgcat ggctatttgc ttgattttgc tagtattgaa gggtaaaggc
    11701 aaaagcatta tcgtttgaaa aatttacctc aaacggttga actgacagtg gatgatgtgg
    11761 aggaggatgt ggatttgacc ctacccgaaa atcgaagtta tcaagaagct gatttttttg
    11821 atactcaatg aaaatcaaag agcaaactag gaagctagcc gcaggctgta cttgagtacg
    11881 gcaaggtgaa gctgacgtgg tttgaagaga ttttcgaaga gtatgaacgt atgtttcgag
    11941 agaagcgcta agtccctctt taaagatttc caagactatc tttcttcatg aggaaagata
    12001 gttttttggt atgattttca ttcccaaaat acaaggggaa tgtgttacaa tagtagtaac
    12061 agataataga aaagagaata gatgagaatt gcagattata gcgtgaccaa ggcagtgttg
    12121 gagcgtcacg gttttacctt taaaaagtcc tttgggcaaa atttcttgac ggataccaat
    12181 atccttcaaa aaattgtgga tacggctgaa attgatgatc aggtcaatgt catcgaaatc
    12241 gggccaggta ttggtgcctt gacagaattt ttggctgagc gtgcagccca agtcatggct
    12301 tttgagattg accaccgttt ggtgccaatt ttggcagata ctctgcgtga ttttgataat
    12361 gtgaccgtag ttaacgaaga tattctcaag gttgatttgg cgcaacatat ccaaaatttt
    12421 aaaaatccta acctgccaat caaggtagtg gctaatttgc cttactacat cacgacgcct
    12481 attctcatgc acttgattga gagtggcatt ccttttagtg agtttgtggt catgatgcag
    12541 aaagaagtag cggaccgcat ttcagcccag cctaacacca aggcttacgg tagcttgtct
    12601 atcgccgtgc agtattacat gacagccaag gttgccttta tcgtgcctcg tacggtcttt
    12661 gtgccagcgc caaatgtgga ttcagccatc ttgaaaatgg tgcgccgtcc agagccagct
    12721 gtagcagtgg aagatgagaa cttcttcttt aaggtttcca aggctagttt tatccatcgc
    12781 cgtaagacct tgtggaataa cttgacaggt tactttggta agactgaaga ggtcaaggac
    12841 aagctgacca aggctttgaa ccaggcaggc ttgtcaccaa gtgtgcgtgg ggaagctctc
    12901 agcttggcag aatttgccgg tctagcagac gcacttaaag ggcaaggact ctaagatgca
    12961 gggacaaatc attaaagcct tggcaggttt ctactatgtg gagagtgatg gccaggttta
    13021 tcaaacacgc gcgcgtggga atttccgtaa aaaaggccat accccttatg ttggggactg
    13081 ggtagatttc tctgccgagg aaaattcaga aggctatatc ctaaaaattc acgaacggaa
    13141 aaacagtctg gttcgtccgc ctattgtcaa tatcgatcaa gctgtagtaa tcatgtccgt
    13201 caaggaacct gattttaaca gcaatttgct ggatcgtttc ttggttcttt tggagcacaa
    13261 gggcatccat cccattgtct atatttccaa aatggatttg ttggaagata ggggagaact
    13321 ggatttttac cggcagacct atggtgacat cggctatgac tttgtgacca gtaaagagga
    13381 actcctgtct ttgttaacag gcaaggttac ggtctttatg gggcagacag gtgttgggaa
    13441 gtcaactctt ctcaataaac tcgtaccaga cctcaatctt gaaacgggag aaatttcaga
    13501 cagtctaggt cgcggtcgcc ataccactcg agctgttagt ttttacaatc tcaacggggg
    13561 taaaatcgca gatacaccag gattttcatc cttggactat gaagtatcaa gggctgaaga
    13621 cctcaatcag gctttcccag agattgctac tgttagccga gattgtaagt tccgtacttg
    13681 tacccatacc catgagccgt cttgtgccgt caaaccagct gttgaagagg gtgttattgc
    13741 aaccttccgt tttgataatt acctgcaatt ccttagtgaa attgaaaatc gtagagaaac
    13801 ctataaaaaa gtcagcaaaa aaattccaaa ataaggagaa acctatgtct caatacaaga
    13861 ttgctccgtc aattctggca gcagattatg ccaactttga acgtgaaatc aaacgtctag
    13921 aagcaactgg ggcagaatat gcccatatcg atatcatgga cagtcatttt gtaccgcaaa
    13981 tcagttttgg tgcaggtgtg gtcgagagcc ttcgtcctca tagtaagatg gttttcgatt
    14041 gccacttgat ggtgtcaaac cctgagcatc atctggaaga ttttgcgcgt gcaggtgcag
    14101 acatcatcag tatccatgta gaagcaacgc ctcatattca tggcgccctc caaaaaattc
    14161 gttcactcgg agttaagcct tcagtcgtta tcaatcctgg cacatcagtt gaagccatca
    14221 agcacgtcct tcatctagtt gaccaagttt tagtcatgac ggttaatcca ggttttggtg
    14281 ggcaagcctt tctgccagaa accatggata aggttcgtga gttggttgct cttcgtgagg
    14341 aaaaaggttt gaactttgaa atcgaagtgg atggtgggat tgatgaccaa actattgccc
    14401 aagctaaaga agctggtgcg accgtttttg tagcaggttc ctatgtcttt aagggagatg
    14461 tcaatgagcg agtacaaact ctcagaaaac aactggacta gggttgccgt ttttgcaggc
    14521 ggagaccgcg gtcattatcg gacagatttt gatgcttttg ttggggtgga tcgaggctcg
    14581 ctctgggtct tggaagaaga cttgcctctt gctctagcag ttggggattt tgattctgtg
    14641 accgaagaag agcgacaggt gattcaaaaa cgtgcccagc attttgtcca agcccgacca
    14701 gaaaaagatg ataccgattt ggaattggct ctcttaacca tctttgaaca aaatcctcag
    14761 gctcaggtta ctattttcgg tgccttgggt ggccgtattg accatatgtt ggccaatgtc
    14821 tttctgccta gcaatcctaa gttggcaccc tatatgcatc aaatagaaat tgaggatggg
    14881 caaaacttga ttacttattg tccagaaggg accagtcagc tagaacctcg ttcagactac
    14941 gactatctag cctttatgcc agttcgggat agccagctga ctattcttgg agccaagtat
    15001 gagttgacag aggaaaattt tttctttaaa aaagtgtacg cttctaacga atatatagat
    15061 agggaagtgt cggtaacttg cccagatggt tatgtggtcg tactgcatag caaggatagg
    15121 aggtaggatg gaaagtttac ttattctatt attaattgcc aacctagctg gtctctttct
    15181 gatttggcaa aggcaggata ggcaggagaa acacttaagt aagagcttgg aggatcaggc
    15241 agatcatttg tcagaccagt tggattaccg ctttgaccaa gccagacaag ccagccagtt
    15301 agaccaaaaa gatttggaag tggctgtcag cgaccgtttg caagaagtgc ggattgaatt
    15361 gcaccaaggc ctgacccaag tccgtcaaga aatgacagat aatctcctcc aaactagaga
    15421 caagacagac caacgtctcc aagccttgca ggaatcaaat gagcaacgtt tggaacaaat
    15481 gcgccagacg gtcgaggaaa aactagaaaa gaccttgcag acacgcttac aggcttcctt
    15541 tgagacagtt tctaaacaac tggagtctgt caatcgtggt cttggagaaa tgcagacagt
    15601 tgcccgtgat gtcggagctc ttaacaaggt tctctctgga accaagacgc gagggattct
    15661 gggagaattg caactggggc aaattattga agacatcatg acacctgccc agtacgaacg
    15721 agaatacgca acggttgaaa actctagtga acgagtggag tatgccatca agttacccgg
    15781 acaaggcgac caagaatacg tctatcttcc aattgactct aagtttccac tggcagatta
    15841 ttaccgcttg gaagaagcct atgagacagg tgacaaggat gagattgaac gctgtcgtaa
    15901 gtcactccta gcaagcgtca agcgctttgc caaggatatc aagagcaagt acatagcacc
    15961 gcctcggaca accaattttg gaatcttgtt tgttccgaca gaaggtctct actcagaaat
    16021 cgtccgcaat ccggtcttct ttgatgattt gagacgggaa gaacagatta ttgttgcagg
    16081 accaagtacc ctatcagccc ttcttaactc cctatcagtt ggtttcaaga cccttaatat
    16141 ccaaaagagt gccgaccata tcagcaagac tcttgccagt gtcaagaccg agtttggcaa
    16201 gtttggtggt attctggtca aggcacaaaa acatctccaa catgcctctg gcaatattga
    16261 tgaattatta aaccgtcgta ccatagctat cgagcggacg ctccgtcaca ttgagttgtc
    16321 agaaggtgag cctgcgcttg atctactcca ttttcaagaa aatgaggaag aatatgaaga
    16381 ttagtcacat gaaaaaagat gagttatttg aaggctttta cctaatcaaa tcagctgacc
    16441 tgaggcaaac tcgagctggg aaaaactacc tagcctttac cttccaagat gatagtggcg
    16501 agattgatgg gaagctctgg gatgcccaac ctcataacat tgaggccttt accgcaggta
    16561 aggttgtcca catgaaagga cgccgagaag tttataacaa tacccctcaa gtcaatcaaa
    16621 ttactctccg cctgcctcaa gctggtgaac ccaatgaccc agctgatttc aaggtcaagt
    16681 caccagttga tgtcaaggaa attcgtgact acatgtcgca aatgattttc aaaattgaaa
    16741 atcctgtctg gcaacggatt gtccgaaatc tctacaccaa gtatgataag gaattctact
    16801 cctatccagc tgccaagacc aaccaccatg cctttgaaac gggcttggcc tatcatacgg
    16861 cgaccatggt gcgtttggca gacgctatta gcgaagttta tcctcagctc aataagagcc
    16921 tgctctatgc ggggattatg ttgcatgact tagctaaggt catcgagttg acggggccag
    16981 accagacaga gtacacagtg cgaggcaatc ttcttggaca tatcgctctc attgatagcg
    17041 aaattaccaa gacagttatg gaactcggca tcgacgatac caaggaagaa gtcgttttgc
    17101 ttcgtcatgt catcctcagt caccacggct tgcttgaata tggaagtcca gtccgtccac
    17161 gtattatgga ggcagagatt atccatatga ttgacaatct ggatgcaagc atgatgatga
    17221 tgtcaacagc tctggctttg gtggataaag gagagatgtc caataaaatc ttcgctatgg
    17281 ataatcgttc cttctataaa ccagatttag attaataatt taagaaaaat gagcattttt
    17341 taggataaga atgttcgttt ttttatgtga atatggtata ataagtaaaa gacaaaaatg
    17401 aatactcttc gaaaatctct tcaaactagg gtagtatcgc cttgtcgtat gtatatatgc
    17461 aggtatatta cagggtttgt cagttctatc gacaatctca aagcagtgtt ttgaaccacc
    17521 agcgaccagc tttctagttt gctttttgat tttttgaata aaaatggaat aggaaataga
    17581 aatgaaatta agaagaagtg atcggatggt tgtcatttcc aactatttga ttaataatcc
    17641 ttataaacta actagtctca atacttttgc tgaaaagtat gagtctgcta aatcatccat
    17701 ctcagaagat atcgtcatta tcaaacgcgc ctttgaggaa attgaaatcg gtcatatcca
    17761 gacagtgact ggggctggcg gaggtgtcat cttcacaccg tctatttcga gtcaggatgc
    17821 taaggaaatg gttgaagact tgcgtaccaa gttatcagaa agtgaccgta tcttgccagg
    17881 tggttatatc tatctgtctg atttgcttag cacaccagcc atcttgaaaa atattggtcg
    17941 tattattgcc aaaagcttta tggaccaaaa aattgacgcg gttatgaccg tagcaactaa
    18001 gggtgtgcca cttgcaaatg cagttgccaa tgtcctcaat gtctcttttg tcattgtgcg
    18061 ccgtgacctg aaaattaccg aaggttcaac tgttagcgtc aactatgttt caggttcaag
    18121 tggtgaccgt atcgagaaaa tgttcctttc aaaacgtagt cttaaggcag gcagccgtgt
    18181 cttgattgtg gatgacttct tgaaaggtgg cggaacggtc aatggtatga ttagtctctt
    18241 gcgcgagttc gactcagaac tggcaggtgt agcggtcttt gcggacaatg cccaagaaga
    18301 acgtgaaaag cagtttgact acaagtcact cttgaaggta accaatattg atgtcaagaa
    18361 ccaagccatc gatgttgagg ttggcaatat ctttgacgaa gataaataag agatagaact
    18421 aaaggttgga acgattgtcc cagcctttct ttgcaaacag aatagaagga agcttatgaa
    18481 aacaccattt atcaatagag aagagttaga agcgattgtt gccgagttcc cgactccctt
    18541 tcacttgtat gatgagaagg ggattcgtga gaaggcaaga gccgtcaacc aagctttttc
    18601 gtggaacaag ggctttaagg aatattttgc agttaaggct actccaactc cagctatttt
    18661 gaaaattctc caagaagaag gttgtggtgt ggactgctct agttatgtag agcttttgat
    18721 gagccataaa ctggactttc tgggttctga gattatgttc tcttccaaca acacgccaga
    18781 caaggaatac gcctatgcac gtgaattggg tgcgaccatt aacttggatg cctttgaaga
    18841 tattgaacat ctggagagag tagcaggcat tccagaaatc atctcttgtc gttataatcc
    18901 tggaggcgtt tttgaactgg ggacagacat tatggacaat cctggggagg ctaagtttgg
    18961 catgaccaag gaccagctct ttgaagtctt tgctatcttg aaggaaaaag gagccaagac
    19021 ttttgggctt cactccttcc tagcgtccaa taccgtgacc catctctatt atccagagtt
    19081 ggctcgtcag ctctttgaac tggctgttga aatcaaggaa aagttgggca tttcgttaga
    19141 ctttatcaat ctttctggcg gtattggtgt taattatcgt ccagaccagg agccgaacga
    19201 tatcgccttg attggtgagg gagttcgtaa ggtgtatgaa gaagttctta cgtcagcagg
    19261 tcttggtcag gtcaagattt tcaccgaatt gggtcgtttt atgctggcac ctcacggtgc
    19321 tctagtcaca agagtcactc ataagaagga aacctaccgt acctatctag gtgtagatgc
    19381 ctcagcagtc aacctcatgc gtccagctat gtacggagct taccatcata ttagcaacgt
    19441 gacccatcca gatggaccag ctgaagcggt agatgtggtt ggttcactct gtgaaaacaa
    19501 tgataaattt gcagtcaatc gcgaactgcc tcatacagaa atcggtgatt tgctggtaat
    19561 tcatgataca ggtgcccacg gattttcaat gggctaccag tataatgcca aattacgttc
    19621 tgcggaaatc ctctataccg aagaaggtaa agcccgtcaa atccgccgtg cagagcgccc
    19681 tgaggactat tttgcaacct tatatggctt cgattttgaa gaataatctg ataatagatt
    19741 gaaaatgaaa ttgaaaaaca gattgctttc taaaaaatag gcaaaaatct tgtttttcct
    19801 tcaattcgtg atataataaa actataaaac gttttcaagg aaggtaacga tatgtctgaa
    19861 gaaacaattg attatggaca agtgacagga atggtgcatt cgacagaaag ctttgggtca
    19921 gtagatgggc ccggtattcg ctttattgta tttttgcagg gctgtcacat gcgttgccag
    19981 tattgccaca acccagacac ttgggctatg gagtccaata agtcacgtga acggacggta
    20041 gatgatgtct tgacagaggc cttgcgctac cgtggtttct ggggaaataa gggtgggatt
    20101 acagtcagtg gaggagaagc tctcttgcag attgatttcc tgattgccct cttcaccaag
    20161 gctaaggaac aaggaatcca ctgtaccttg gacacctgtg ctcttccttt ccgtaataaa
    20221 ccacgttacc ttgagaagtt tgacaaactc atggctgtca ctgacttggt tcttttggat
    20281 atcaaggaaa tcaacgaaga acagcacaag attgtcacta gccaaaccaa taaaaatatc
    20341 ttggcttgtg cccagtatct atcagatatt ggaaaacctg tctggattcg ccacgtgcta
    20401 gttccaggat tgacagacag agatgatgac ttgattgaac ttggtaagtt cgtcaagacc
    20461 ctcaaaaatg ttgataagtt tgaaattcta ccttatcaca ccatgggtga gttcaagtgg
    20521 cgtgaacttg gaattccata ttccctcgaa ggagtcaaac caccaacagc agatcgcgtc
    20581 aagaacgcta aacaactcat ggataccgaa agttatcaag attatatgaa acgtgtacat
    20641 ggatagaaaa gaagcctgat ggaaacatcg ggcttttgat ttgcaaaaag acttagcaaa
    20701 tcagctaagc ctttttcttc ttatctcgaa cgttgttttc cagcgttgcg atttttgtgt
    20761 tttttcttgc ttgtgatagc agttggttgt tcaggggtaa cgtcttttcg tccacttggt
    20821 gttgagaaag cacttgcttt tgatgggttc tttgccagtt cttcacggac ttttttgcga
    20881 agttttggac gaacgatata gttgacgata aattgctgga gaatcatcat gaaaccaccg
    20941 acaacccagt aaagtgtgac actagctggt gagaagaggg agaagacgac gatcatgagt
    21001 gggctcatgt aaatcatttt cttgatttgt tctctttgca tttcatcttc tactccgtga
    21061 agtgaaagga gcgattgaag atagtaaagg acaccagcac aggcaaccaa aatcatactt
    21121 ggagaaccta gaggaatgcc taggtagctt gcttgagcaa ccccttcagt atgttgggca
    21181 gcaaagtaga tagcagagaa gaaaggcatt tgaaggagga tagggaaaca tcctacaccg
    21241 ccaaacatgc tgataccgtg ctctttttga gcggcaaaga gggcttgttg ggcttcgagt
    21301 ttttcttctt gagtagtcgc ttctttgaga cgcgtttggt gtggctcaag gacgtgcttg
    21361 agggcgttca tcttttcaga gtgaagcgtt gccttccatg attggtagat accaagtggt
    21421 aagataatca agcgtacgat aatggttacg ataatgatag cgacaccaaa gcctagacct
    21481 ttatcagtag cgaagtactt gatggcttca gccataggcg ctccgatcgt attccaaata
    21541 aatcctgttg gctgacctgt ggttttatcg acattgacac agccagtcaa gacaagcaac
    21601 atagccactc ccatagccga gagtgcaaaa cgtttaatag atttcactgt ttttattcct
    21661 tctttaaaaa ttataccttt ctattctact gtttttttac aaaatataca atagttctgg
    21721 agacctaatt tgcgatttta aagtcagggt agggaggaaa gttgctcagt ttgacatcta
    21781 agtaactgac ttttgaaaaa ggtgtcggtc cttttcggat ttcttggata aattttgcca
    21841 taatagctga tgagtctgct tgggctaaga tttccactgt gccatcgtcg ttattccata
    21901 cccgacctgt gatgccaccg atttcaaggg ccaggctata aacaccccaa cgaaagccga
    21961 ctccctgcac cctaccttgg gcaatcattc taaccttttg cataccaaac ccctttgatt
    22021 gtgttataat atttctatga ctattataac ctcaaaagcc aattctgtgg taaaaaatac
    22081 caagaaatta caccaaaaaa aataccgcaa gtcagcctat ttgattgaag gctggcactt
    22141 gtttgaagaa gctgttcaag ctggagtgac gattgagaaa atctttaccc tagaaaatta
    22201 tcgagatcag ttagcaactt tttcgcaaac tgtctgggtg tcagaggata ttttgctaga
    22261 tttggcggat tctcagactc cacagggaat tgtggccgtg gttcaaaaag aagaagtagg
    22321 acaagctgat ttgagtcagg gcaagttctt gtttttggaa gatgtgcaag atcctggtaa
    22381 tgtaggaact atcattcgaa ctgcggatgc agcaggtttt acaggagtga ttgtttcaga
    22441 taagtcggca gacatctaca gtctcaaaac cctacgttcc atgcaaggta gtcatttcca
    22501 tctgcccatt taccggatga ctagtcaagc gcttcttgac gaaactaaaa aggtagctat
    22561 cccagtgcta gcaacaaccc tatctaaaga ttctgttgat tacagagaac tgccttctat
    22621 agaaaatttt gtactagtta tgggaaatga gggtcaagga attagtcccc ttatggctga
    22681 aagtgcagac cagttggtcc atattagcat gaaggggcag gccgagagtt tgaatgttgc
    22741 agttgcagcc ggtattttaa tcttccattt aagctaattt taactttttt tgttataatc
    22801 aaggaaagat gttcacagaa aaggagaaaa ggatgaatca cactattata catgaccgtg
    22861 caggtctcaa tcaattttac gctaaggttt atgcctttgt tggtctggga atcggactat
    22921 ctgctttggt atcaggcctt atgttgacgg tctttcagtc tcagttggtt tacttgttga
    22981 tgcaggggcg tctctggttg accattgcta cttttgcgga gttggctttg gttttcgttg
    23041 ccagtagcat ggcttcaaga aatagtccag ctgctcttcc agtattttta ctttactccg
    23101 ttttaaacgg ctttaccctt agttttgtgg tggctttcta tactccgggt acagttttat
    23161 ctgcctttgt atcgagcgcc cttctcttct ttgtcatggc ggcagttggt atctttacca
    23221 agaaagactt gagtgggatt ggtcgagcaa tgatggcagc tctcattggt ttactgattg
    23281 ctatggtagt caatattttc ttggctagtg gcttctttga ttatatgatt agcgtagcca
    23341 tggttttggt tttttcaggg ttgattgctt gggataacca aaagattcgc ctcgcctatg
    23401 aacaatcaca aggtcgtgta gcgacaggct gggtggtgtc aatggctctc agcatctatc
    23461 ttgattttat caatcttttc ctaagtattc tacgtatctt tggtcgcaat gactaaaagg
    23521 tctatgaaat cagtgttttg agtgacactg atttttttta aattttctct tttcttttct
    23581 tggaaatagg tgtataatac gtttattaaa tttttgagga gttgtctatg aagaaaagtt
    23641 ttatccatca acaagaagaa atttcctttg tcaaaaacac ttttacccag tatttgaaag
    23701 ataagctaga agttgtcgaa gttcaaggtc ctatcttgag taaggtcggt gacggaatgc
    23761 aggacaacct gtctggtgtg gaaaatccag tatcggtcaa ggttctccaa atccctgatg
    23821 ctacttatga agtggtgcac tcacttgcta aatggaaacg ccacaccttg gctcgttttg
    23881 gctttggtga aggagagggt ctctttgtcc acatgaaagc ccttcgtcca gatgaggatt
    23941 ccttggatgc aacccactct gtttatgttg accagtggga ctgggagaag gttatcccaa
    24001 atggtaagcg taacatcgtt tatctaaaag aaacagttga gaagatttat aaggctattc
    24061 gcctgactga gctagctgtt gaagcccgct atgacatcga gtctatcttg ccaaaacaaa
    24121 tcacctttat ccatacagaa gaattggtag aacgctaccc agacttgaca ccgaaagaac
    24181 gtgaaaatgc gatttgtaaa gaatttggag ccgtcttttt gattggtatc ggtggcgagt
    24241 tgccagatgg taaaccgcac gatggacgtg caccagacta tgatgactgg acaagcgagt
    24301 ctgagaatgg ctacaagggt ctaaatggtg atattcttgt ctggaatgag tctttaggtg
    24361 gagcctttga gttgtcttct atgggaattc gtgtagatga agaaactctt agacgtcagg
    24421 tggaaattac aggtgatgaa gaccgcttgg aattggaatg gcacaagtct ttgttgaatg
    24481 gtctattccc attgacaatc ggtggaggaa ttggacaatc tcgtatggcc atgttcctac
    24541 ttcgcaagag acacatcgga gaagtgcaaa caagtgtttg gcctcaagaa gtccgcgata
    24601 cttacgaaaa tattttgtag agaatcgaac cgcaaggttc ggttttcttt ctctttttgt
    24661 ctataatttg gtataataaa cagtatgaaa atcgtatcag gaatctatgg gggacgtccc
    24721 ctcaagacac tagaaggcaa gacgacaaga cctacttcgg ataaggttag gggagccatt
    24781 tttaacatga ttggtcccta ctttgaagtg ggacgagtct tggaccttta tgcaggtagt
    24841 ggtggtttat ctatcgaagc agtatcgcgt ggcatgtcca gtgctgtttt ggtggagcga
    24901 gaccgtaagg ctcagaccat cgtggctgaa aatatccaga tgaccaagga agttggaaaa
    24961 tttcaactcc tcaagatgga tgcagaaagg gcattggaac aggtatctgg ggaatttgac
    25021 ctcgttttct tagaccctcc ctatgccaag gaacaaatcg tagcagatat tgaaaaaatg
    25081 gctgagagag agcttttttc tgaagatgtt atggttgtgt gcgagacgga taaagccgtt
    25141 gaacttccag aagagattgc ctgtctgggt atctggaagg aaaagattta tggaattagt
    25201 aaggtgacag tctatgtcag ataagattgg cttattcaca ggctcatttg atccgatgac
    25261 aaatgggcat ctggatatca ttgaacgggc gagcagactt tttgataagc tttatgtggg
    25321 tatttttttt aatccccaca aacaaggatt tctccctctt gaaaatcgta aacgggggct
    25381 agaaaaggct ttgggacatt tggaaaatgt tgaagtcgtg gcttctcatg ataaattggt
    25441 ggtcgatgtt gcaaaaagac tgggggctac tttcctagta cgggggttga ggaatgcggc
    25501 ggatttgcaa tatgaagcta gttttgatta ctacaatcat cagctgtctt ctgatataga
    25561 gactatttat ttacatagcc ggcctgaaca tctctatatc agttcatcag gtgttcgaga
    25621 gcttttgaag tttggtcagg atattgcctg ccatgttccc gagagtattt tggaggaaat
    25681 aagaaatgaa aaaaaagatt agatggcctt tgtatgtcat tgcggccttg attgtgacct
    25741 tcttggcatt tgtggtgcct ttgccctact atatagaggt tccaggtggt tcggaagata
    25801 ttcgccaagt ccttaaagta aatgacacag aagataagga agctggtgcc tatcaattcg
    25861 ttacggttgg tgttcaacat gctactttag ctcatatgat ttatgcttgg ttgacacctt
    25921 ttacagatat tcgtagtgct caggagacta caggtggttc ttccgatgtt gaatttatgc
    25981 gaatcaatca attctacatg caaacatcgc aaaatatggc caagtatcaa ggactaaaaa
    26041 cagctggtaa ggatatcgaa ctcaagtact ttggagttta tgttttgaat gtgacggata
    26101 attcaacctt taaagggatt ctcaatatct ctgatacagt cacagcagtc aatgatcaga
    26161 cctttgatag ttccaaagac ttgattgatt acgtcagttc tcaaaaatta ggggattccg
    26221 tcaaggtcac ctatgaagag gatgggcaaa ccaagtctgc agaaggaaag attatcacct
    26281 tggaaaatgg caaaaatgga attggaatcg gcttgattga ccgtacagag gtaatcagca
    26341 atgtcccaat tagcttttca acagctggta ttggcggtcc aagtgctggt ctcatgttta
    26401 gtctagctat ctatactcaa atagctcacc cagatcttcg taatggtcgt attgttgccg
    26461 gtacaggtac cattgaccgc gatggtaatg tgggagacat tggaggtatt gataagaagg
    26521 ttgtagcttc ggctagggca ggtgctgcta ttttctttgc tcctgataac cctgttagcg
    26581 aagaagaaca aaaggcgcat ccggacgcga aaaacaacta ccaaacagcc ctagaagcgg
    26641 ctaaaacaat caagacggat atgaaaatcg tgcccgttaa aaccctacaa gatgcgattg
    26701 attacttgaa aaacaatccc taatttttga gtgaagtttc caaactagcg cacaaacaga
    26761 gaagatggta taatagtcaa atggttcaat tattattcac tctaagtagt catatgttct
    26821 ttatttatgt gagtttttac cttttaaagg atcttgttag atgggagaag gttttaaaag
    26881 tgacagatga gaatacaaga aaagttcgtt tattagtagc cttttttagc attgtcatag
    26941 gctacatcct gagttctttc tttattagcc tgtatcattt gtggcaagaa gcgcttagag
    27001 gattattatg aaatcaagag taaaggaaac gagtatggat aaaattgtgg ttcaaggtgg
    27061 cgataatcgt ctggtaggaa gcgtgacgat cgagggagca aaaaatgcag tcttaccctt
    27121 gttggcagcg actattctag caagtgaagg aaagaccgtc ttgcagaatg ttccgatttt
    27181 gtcggatgtc tttattatga atcaggtagt tggtggtttg aatgccaagg ttgactttga
    27241 tgaggaagct catcttgtca aggtggatgc tactggcgac atcactgagg aagcccctta
    27301 caagtatgtc agcaagatgc gcgcctccat cgttgtatta gggccaatcc ttgcccgtgt
    27361 gggtcatgcc aaggtatcca tgccaggtgg ttgtacgatt ggtagccgtc ctattgatct
    27421 tcatttgaaa ggtctggaag ctatgggggt taagattagt cagacagctg gttacatcga
    27481 agccaaggca gaacgcttgc atggtgctca tatctatatg gactttccaa gtgttggtgc
    27541 aacgcagaac ttgatgatgg cagcgactct ggctgatggg gtgacagtga ttgagaatgc
    27601 tgcgcgtgag cctgagattg ttgacttagc cattctcctt aatgaaatgg gagccaaggt
    27661 caaaggtgct ggtacagaga ctataaccat tactggtgtt gagaaacttc atggtacgac
    27721 tcacaatgta gtccaagacc gtatcgaagc aggaaccttt atggtagctg ctgccatgac
    27781 tggtggtgat gtcttgattc gagacgctgt ctgggagcac aaccgtccct tgattgccaa
    27841 gttacttgaa atgggtgttg aagtaattga agaagacgaa ggaattcgtg ttcgttctca
    27901 actagaaaat ctaaaagctg ttcatgtgaa aaccttgccc cacccaggat ttccaacaga
    27961 tatgcaggct caatttacag ccttgatgac agttgcaaaa ggcgaatcaa ccatggtgga
    28021 gacagttttc gaaaatcgtt tccaacacct agaagagatg cgccgcatgg gcttgcattc
    28081 tgagattatc cgtgatacag ctcgtattgt tggtggacag cctttgcagg gagcagaagt
    28141 tctttcaact gaccttcgtg ccagtgcagc cttgattttg acaggtttgg tagcacaggg
    28201 agaaactgtg gtcggtaaat tggttcactt ggatagaggt tactacggtt tccatgagaa
    28261 gttggcgcag ctaggtgcta agattcagcg gattgaggca agcgatgaag atgaataaga
    28321 aatcaagcta cgtaatcaat cgtttacttt tagtcatcat agtactgatt ttaggtactc
    28381 tggctctagg aatcggtcta atggtaggtt atggaatctt gggcaagggt caagatccat
    28441 gggctatcct gtctccagca aaatggcagg aattgattca taaatttaca ggaaattagg
    28501 ctggagaacc agcctttttc taaagataag gagaaatatg aacaaaaaaa caagacagac
    28561 actaatcgga ctgctagtgt tattgctttt gtctacaggg agctattata tcaagcagat
    28621 gcagtcgaca gctaatagtc ctaaaaccaa gcttagtcag aaaaaacaag cgcctgaagc
    28681 tcctagtcaa gcattggcag agagtgtctt aacagacgca gtcaagagtc aaataaaggg
    28741 gagtctggag tggaatggct caggtgcttt tatcgtcaat ggtaataaaa caaatctaga
    28801 tgccaaggtt tcaagtaagc cctacgctga caataaaaca aagacagtgg gcaaggaaac
    28861 tgttccaacc gtagctaatg ccctcttgtc taaggccact cgtcagtaca agaatcgtaa
    28921 agaaactggg aatggttcaa cttcttggac tcctccaggt tggcatcagg tcaagaatct
    28981 aaagggctct tatacccatg cagtcgatag aggtcatttg ttaggctatg ccttaatcgg
    29041 tggtttggat ggttttgatg cctcaacaag caatcctaaa aacattgctg ttcagacagc
    29101 ctgggcaaat caggcacaag ccgagtattc gactggtcaa aactactatg aaagcaaggt
    29161 gcgtaaagcc ttggaccaaa acaagcgtgt ccgttaccgt gtaacccttt actacgcttc
    29221 aaacgaggat ttagttccct cagcttcaca gattgaagcc aagtcttcgg atggagaatt
    29281 ggaattcaat gttctagttc ccaatgttca aaagggactt caactggatt accgaactgg
    29341 agaagtaact gtaactcagt aaaagataag cctaaactcc tatgtcactt atggatgtag
    29401 gagttctttt tactagttta agcagggcta gaacaggtac taagaaaaaa tagtaacttc
    29461 taaaactaac ttccagtttc ccacaaccta gcttttagta tgagaaaaac gcgtgaaatc
    29521 agtggaaatc cgtcttagac taacttccag ttttgggaga gagatggaag ttacgctaag
    29581 aaaaaaataa tatgtattgg attaaatttt gtgatataat aaaggataag atactatttt
    29641 aggagaagaa ctatggaaga cccaagtagt cagaatttgt tgctacaatt tgttttattg
    29701 tttatcttga cggtattaaa tgcctttttc tcagccacag aaatggccat ggtttctctt
    29761 aatcgtgccc gagttgaaca aaaggcggaa gaaggagaca gacgctacat ccgcctgctg
    29821 aaggtactag aaaatcctaa ccacttttta tcaaccattc aggtaggaat caccctgatt
    29881 acaatcttat caggggcgag tttaactgat actctaggac gtgtgattgc ctcttggctt
    29941 gggaatggcg aaacagcaca agccgtggca acttttctat ccttggcatt tttgacctat
    30001 atttccatcg tttttgggga attatatcct aagagaatcg ctcttaatct aaaggatgcc
    30061 ttggcaattc gtacagcgcc gattatcatt gggatcggaa aactagtcag tccttttgtt
    30121 tggcttttag ctgcttcgac caatttcttg agccatttga ctcctatgtc attcgatgat
    30181 gcggatgaaa agatgacccg tgatgaaatt gcctatatgc tgacaaatag tgaagaaaca
    30241 ttggatgctg atgagattga gatgctacaa ggtgtctttt cgctcgatga actgatggca
    30301 cgagaggtta tggttcctcg aacggatgcc tttatggtgg atattcagga tgatagtcaa
    30361 gccattatcc aaagtatttt aaaacaaaat tattctcgta tcccggttta tgatggggat
    30421 aaggacaatg taattggaat cattcacacc aagagtctcc ttaaggcagg ctttgtggac
    30481 ggttttgaca atattgtttg gaagagaatt ttacaagatc cactttttgt acctgaaact
    30541 atttttgtgg atgacttgct aaaagaactg cgaaataccc aaagacaaat ggcaatcttg
    30601 ctggatgaat acggtggtat ggctggtttg gtgactttgg aagacctctt agaggaaatt
    30661 gttggtgaaa ttgatgatga aacggacaaa gcagcaatag atgttcatca gattggcgag
    30721 gatacctaca ttgttcaagg aactatgacc ctcaatgatt tcaataatta ctttgatgtt
    30781 gaactggaaa gtgatgacgt tgataccatc gctggttatt atttgacagg agtgggaacc
    30841 attccaacga ctgagaaact cagctatgaa ttggtcagcc aaaacaaaca gtttatctta
    30901 accaatgata aagtgaagaa tggacgtgtt accaaggtaa aagtccaaat cacagaagtc
    30961 gaaatagaag aagaaacaga ataaactaga ggcttttgtc accaggcgtg acgaagcttt
    31021 tttcaattga tttcaaaagg gaattatgcc atggaaagaa ctctgccaca aactcgctcc
    31081 aaaagttttt aaagttatac gtatttattc gagggaaaat aaaaaaagcc cttcaaattg
    31141 ggctttctgt tcatttgaaa cctgataaat caagttatag aaggcggtag acggatttga
    31201 accgacgatc aagcttttgc agagccgtgc cttaccactt ggctataccg ccttaacgtt
    31261 tattattata ccttgaaaat gattttcagt caatagtttt ttcagttttt tgtaccatag
    31321 cgtgattcga atgtgatgcc aagttttcta tgggaaaaaa tttaccaaaa acctactgaa
    31381 accccttgaa aaatctagct aaatagggta taatatgggt aatcatttgt cgtaggtttt
    31441 gtctgaaata ttgtccagac aaggctcaca gcagttaaat cttctgaaaa agtcagattt
    31501 agctgctctt tttgtgcttt ttttcaagat tttgagcatt tgtaacagag gcttaaagat
    31561 tctgaaaatt cgtcaagagg acacggtgat aaggggttta caaccatatg gcgattagaa
    31621 aagcctgatt gacaaggctt ggaacttatt tacaaaggag aatcatcttg gcaggacatg
    31681 acgttcaata cgggaaacat cgtacccgtc gtagtttttc aagaatcaaa gaagttcttg
    31741 acttaccaaa tttgattgaa attcaaactg actcattcaa agctttccta gaccacggtc
    31801 ttaaggaagt gtttgaagat gtattgccaa tttcaaactt cacagacaca atggagttgg
    31861 aatttgttgg atatgaaatc aaggaaccaa aatacacgct agaagaagct cgtatccacg
    31921 atgctagcta ctcagcacca atttttgtaa ccttccgctt gatcaataaa gaaacaggcg
    31981 aaatcaagac ccaagaagtt ttctttggtg atttcccaat catgacagaa atgggtactt
    32041 tcatcatcaa tggtggtgaa cgtattatcg tttctcagtt ggtccgctca ccaggtgttt
    32101 actttaacga caaagtagac aaaaatggta aggtgggcta tggttcaact gttatcccta
    32161 accgtggagc ttggttggaa cttgaaagcg actcaaaaga tatcacctac actcgtatcg
    32221 accgtactcg taagattcca tttacaacct tggttcgtgc tcttggtttc tcaggtgatg
    32281 atgaaatctt tgatatcttt ggtgacagcg aattggttcg caacactgtt gaaaaagata
    32341 tccacaagaa tccaatggac tctcgtacag acgaagcctt gaaagaaatt tacgaacgcc
    32401 ttcgtccagg tgagcctaag acggctgaaa gctcacgtag cttgcttgta gctcgcttct
    32461 ttgacccacg tcgttatgac ttggcagcag ttggtcgtta caaaatcaat aaaaaactca
    32521 atgttaaaac acgtttgctc aaccaaacca ttgcagagcc attggtagac cctgaaactg
    32581 gagaaatctt ggtagaagct ggtacgatta tgactcgtag cgtgattgaa agcattgaaa
    32641 gccatttgga tggcgacttg aacaagattg tctacatccc aaacgatgca gccgttgtga
    32701 ctgagcctgt tgttcttcaa aaattcaagg ttattgctcc aactgatcca gatcgcgtcg
    32761 taacgatcat tggtaatgct aacccagatg acaaggttcg tacggtgact cctgcagata
    32821 tccttgctga gatgagctac ttcctcaact tggctgaagg acttggccgt gtagatgata
    32881 tcgaccacct tggaaatcgt cgtatccgtg cggttggtga attgcttgcc aaccaagtac
    32941 gtttgggact ttctcgtatg gaacgtaatg tccgtgaacg tatgtctgtt caggacaatg
    33001 aagtcttgac accacaacaa attatcaata tccgtcctgt aacagctgca gttaaagaat
    33061 tctttggttc atcacagttg tcacagttca tggaccaaca caacccgctt tctgagttgt
    33121 ctcacaaacg ccgtttgtca gccttaggac ctggtggttt gactcgtgac cgtgctggat
    33181 atgaagtacg tgacgtgcac tacactcact atggtcgtat gtgtccaatc gagacacctg
    33241 aaggacctaa catcggtttg atcaataact tgtcatctta tggacacttg aacaaatatg
    33301 gttttgttca aacaccatac cgtaaggttg accgtgaaac aggtgttgtc acgaacgaaa
    33361 ttgtttggtt gacagctgat gaagaagatg aatatactgt agctcaggct aactctcgtc
    33421 tgaatgaaga tggaaccttt gctgagaaga ttgtcatggg acgtcaccaa ggggtcaacc
    33481 aagagtatcc agctaatatt gttgactaca tggacgtttc accaaaacag gtagttgccg
    33541 ttgcgacagc atgtattcct ttcttggaaa acgatgactc caaccgtgcc ctcatgggag
    33601 ccaatatgca acgtcaggct gtgccattga ttaatcctca ggcaccttac gttggtactg
    33661 gtatggaata ccaagcagcc cacgattctg gtgcggctgt gattgctcag tatgatggta
    33721 aagttactta cgcagatgct gacaaggtag aagttcgtcg tgaagatggt tcattggatg
    33781 tttaccacat ccaaaaattc cgtcgttcaa actcaggtac tgcttacaac caacgcactc
    33841 tcgtaaaagt tggtgatgtc gttgaaaaag gcgatttcat cgctgacgga ccttctatgg
    33901 aaaatggaga aatggcgctt ggacaaaacc caatcgttgc ctacatgact tgggaaggtt
    33961 acaacttcga ggatgccgtt atcatgagcg aacgcttggt gaaggacgat gtctacacat
    34021 ctgttcacct tgaagaatac gaatcagaaa cgcgcgatac aaagcttggg cctgaagaaa
    34081 tcactcgcga aattccaaac gttggtgaag atgccctcaa agaccttgac gaaatgggga
    34141 ttatccgtat tggtgctgag gttaaagaag gtgatattct tgtaggtaaa gtaacaccta
    34201 agggtgagaa agatctttca gctgaagaac gtctcttgca cgctatcttt ggagacaagt
    34261 ctcgtgaagt gcgtgatact tctcttcgtg taccacacgg tgccgatggt gtcgttcgtg
    34321 atgttaagat ctttacacgt gtaaatggag atgagttgca atcaggtgtt aacatgttgg
    34381 ttcgtgttta catcgctcaa aaacgtaaga ttaaggtcgg agataaaatg gccggacgtc
    34441 acggaaacaa aggggttgtc tctcgtatcg ttcctgtaga agacatgcct taccttccag
    34501 acggaactcc agtcgacatc atgttgaacc cacttggggt accatcacgt atgaatatcg
    34561 gtcaggttat ggagcttcac cttggtatgg cagctcgtac tcttggtatt cacatcgcta
    34621 caccagtctt tgatggagca agttctgaag atctttggtc aactgttaaa gaagcaggta
    34681 tggatagcga tgccaaaacg atcctttacg atggacgtac tggtgaaccg tttgataacc
    34741 gtgtttctgt cggagtcatg tacatgatca aactccacca catggttgat gataaattgc
    34801 acgcgcgttc agtcggacct tactcaactg ttacccaaca accactcgga ggtaaagctc
    34861 agtttggtgg acaacgtttc ggtgagatgg aggtttgggc tcttgaagcc tacggtgcgt
    34921 caaatgtcct tcaagaaatc ttgacttaca agtcggacga tatcaacgga cgtttgaaag
    34981 cctatgaagc tattacaaaa ggcaaaccaa ttccaaaacc aggtgttcca gaatccttcc
    35041 gagttcttgt caaagaattg caatctcttg gtcttgacat gcgtgtccta gacgaagatg
    35101 accaagaagt ggaacttcgc gacttggatg aaggaatgga cgaagatgtc atccacgtag
    35161 atgaccttga aaaagcccgc gaaaaagcag cccaagaggc taaagcagcc tttgaagctg
    35221 aagaagctga gaaagcaaca aaagcggaag caacagaaga agctgctgaa caagaataag
    35281 cagttcactt agaatagaaa gggaagaaat agtggttgat gtaaatcgtt ttaaaagtat
    35341 gcaaatcacc ctagcttctc caagtaaagt ccgttcatgg tcttatggag aagtcaaaaa
    35401 acctgaaaca atcaattatc gtaccttgaa accagaacgt gaaggactct ttgatgaagt
    35461 gatctttggt cctacaaaag actgggaatg tgcttgtggt aagtacaaac gcattcgtta
    35521 cagaggaatt gtttgtgacc gctgtggggt tgaagtaacg cgtacgaaag ttcgtcgtga
    35581 gcgtatggga catatcgaat tgaaagctcc tgtatctcac atctggtact tcaaggggat
    35641 tccaagccgt atgggcttga cccttgatat gagccctcgt gccctcgagg aagttatcta
    35701 ctttgcggct tatgtggtga ttgatcctaa ggatacacca cttgagcaca agtctatcat
    35761 gacagagcgc gaataccgag agcgcttgcg tgaatatggt tatggttcat ttgttgccaa
    35821 gatgggtgcg gaagccatcc aagacctttt gaagcaagta gatcttgaaa aagaaattgc
    35881 tgaactcaaa gaagaattga aaacagctac tggacaaaaa cgtgtcaaag ccatccgtcg
    35941 tttggatgtt ttggatgcct tttacaagtc tggaaacaaa cctgaatgga tgattcttaa
    36001 catccttccg gttatcccac cagatcttcg tccaatgttg cagttggatg gtggccgttt
    36061 tgcctcatct gacttgaatg acctttaccg ccgtgttatc aaccgtaaca accgtttggc
    36121 tcgtttgctt gagttaaatg caccaggtat catcgttcaa aatgagaagc gtatgcttca
    36181 agaagcagtt gatgctttga ttgacaatgg tcgtcgtggt cgtccaatca caggaccagg
    36241 tagccgtcca ttgaaatcat tgagccacat gcttaaaggt aaacaaggac gcttccgtca
    36301 aaacttgctc ggtaaacgtg ttgacttctc aggacgttcc gttatcgccg ttggtccaac
    36361 tcttaagatg taccaatgtg gtgtgccacg tgaaatggcg attgaactct ttaaaccatt
    36421 tgtcatgcgt gaaatcgttg cccgtgatat cgtgcaaaac gtcaaagcag ctaaacgctt
    36481 ggtggaacgc ggagatgagc gtatctggga tatccttgaa gaagtgatta aagaacaccc
    36541 agtgcttttg aaccgcgcac cgacccttca ccgtttgggt atccaagcct tcgagccagt
    36601 cttaattgat ggtaaggctc ttcgcttgca cccacttgtc tgtgaagcct acaatgctga
    36661 ctttgacggg gaccaaatgg ccatccacgt accactttca gaagaagcac aagcagaagc
    36721 tcgtatcctc atgctagctg ctgagcacat cttgaacccg aaagatggga aaccggtagt
    36781 tactccatct caggacatgg ttttgggtaa ctactacttg accatggaag aagctggtcg
    36841 cgaaggtgaa ggaatggtct tcaaagaccg tgacgaagcg gttatggctt accgcaatgg
    36901 ttatgttcac ctccactcac gtgttggtat cgcaacagac agcctcaaca agccttggac
    36961 agaagagcaa agacataagg tcttgcttac aacagttggt aaaattctct tcaacgatat
    37021 catgccagag gggctaccat acttgcaaga accaaacaat gccaacttga cagaaggtgt
    37081 tccagctaaa tacttcttgc cacttggtgg agatatcaag gaagctatca gcaatcttga
    37141 gctcaaccct ccattcaaga agaaaaacct tggaaatatc atcgctgaaa tcttcaaacg
    37201 tttccgtacg acagaaactt ctgccctact tgaccgcatg aagaacctcg gttaccacca
    37261 ctcaactctt gcaggattga cagtgggtat tgccgatatc ccagtcgttg atgacaaggc
    37321 tgaaatcatt gaagaatcac acaaacgtgt cgaacaaatc accaaacaat tccgtcgtgg
    37381 tatgatcaca gacgacgagc gttacaatgc tgttacagct gaatggcgtg ctgcccgtga
    37441 aaaacttgag aaacgcttga ttgctaacca agatcctaag aacccaatcg ttatgatgat
    37501 ggactctgga gcccgtggta acatctcaaa cttctcacag cttgccggta tgcgtggtct
    37561 gatggctgct ccgaatggac gtatcatgga attgccaatc ctttcaaact tccgcgaagg
    37621 tttgtcggta ttggaaatgt tcttctcaac tcacggtgct cgtaaaggta tgaccgatac
    37681 ggcccttaag acagccgact caggttactt gactcgtcgt ttggtcgacg ttgcccaaga
    37741 cgttatcatc cgtgaggacg actgtggaac tgaccgtggt ctcttgatcc gttctatcgc
    37801 agaaggaaaa gagatgatcg agtctctcga agagcgtctc aacggtcgtt acactaagaa
    37861 aactgttaaa catccagaaa ctggtgcagt gattattggt ccaaatgaat tgattacaga
    37921 agacaaggcg cgtgaaattg tcaatgctgg tgtggaagaa gtgactatcc gttctgtatt
    37981 tacatgtaac actcgtcacg gtgtctgccg tcactgttat ggtatcaact tggcgactgg
    38041 tgatgcggtt gaagttggtg aagcagttgg tacaatcgct gcccaatcta tcggggaacc
    38101 tggtacacag cttacaatgc gtaccttcca cacaggtggg gttgcctcaa ataccgatat
    38161 cactcagggt cttcctcgtg tccaagaaat ctttgaagcc cgcaatccta aaggggaagc
    38221 ggttatcaca gaggttaaag gacaagttac tgctatcgaa gaagatgcat caactcgtac
    38281 caagaaagtc tttgttaagg gtgaaactgg cgaaggtgaa tatgtcgttc catttacagc
    38341 tcgtatgcgt gtcgaagttg ggggccaagt agcgcgtggt gctgctctga cagaaggttc
    38401 tatccaacca aaacgtctcc ttgcagttcg tgatgtcttg tcagttgaaa cgtaccttct
    38461 cggtgaagta caaaaagttt accgtagcca aggggtagaa atcggtgaca aacacatcga
    38521 ggtaatggtt cgtcaaatga tccgtaaagt ccgtgtcatg gatccaggtg atacagatct
    38581 tctcatgggt accctcatgg atatcaatga ctttacagat gccaacaaag atgtccttat
    38641 cgcaggtgga gttccagcga caggtcgccc agtccttatg ggaattacca aagcctcact
    38701 tgaaacaaac agtttcttgt cagcggcttc cttccaggaa acaactcgtg tccttactga
    38761 cgcagctatc cgtggtaaga aagaccatct ccttggactt aaagaaaatg ttatcatcgg
    38821 taagatcatc ccagctggta ctggtatggc ccgttaccgt aaccttgaac cacatgctgt
    38881 caacgaagaa gaatacctta atcctccagt agaggaagaa ggaaatgaag aaacaacaga
    38941 agtagttgtg gatactgccg ttgaaactgt ggaagaaaca gtagaataag agagaatgag
    39001 agaaccctcg ggttctctca ttctctcttc tgaaaacttt ctccattttt ccacgaaatg
    39061 tggtaaaata aaagaaagaa gctgacaaag gagacgggta tggaacaaac attctttatc
    39121 atcaaaccag atggtgtaaa aagagggcta gtgggtgaag tgttaaagcg catcgaacaa
    39181 cgtggattta caatcgaaaa attggagttt cgttcacagg tttcagaaga gttgattgac
    39241 cagcactatc aggacttggt tggtcagagt ttttacccac cgattcgtga attcatgact
    39301 tcaggtccag ttcttgtggg tgtcatttct ggtcccaaag taatcgaaac ttggcggacc
    39361 atgatgggtg caactcgtcc agaagaagct ttaccaggca ctattcgagg tgattttgca
    39421 aaagctgctg gagaaaatga gattatccaa aatgttgtac atggttcaga ttcagaagag
    39481 tcagctaagc gagaaattgc tctttggttt taagagtgga ttggctcaat caattggtta
    39541 aaagctcatt tgaatagaaa gtatagtcaa ttagtttaag acatgacgca tgatatcaaa
    39601 ctttttagtt tttgatatgg tgcgtttttt gattaagtga ctattagttt gtctcgctcc
    39661 gttaggtgcg agacaaaaaa aacacccgct atgcgggtgc gcgtcgaagg ttataccaaa
    39721 aaaactccaa acgcgataca ataaaggtgt tcaatccaat tgtaaagcga aaggagaaaa
    39781 atatggcaca aaaggttctt ttatcctgtt cttgtttcaa ttgactatat ttctatactt
    39841 tttcttcctt tctctgtgaa aatagcaagt ttttcccacc ttttctcaat actgaaacaa
    39901 catctttcca gaacttcaat aatttctttt tgtgttaaat cattatttaa ctaaatcttt
    39961 ggaggcaata cgttttcaaa gctacttttt taccatagat tttgagcctt caacacagat
    40021 tactaagcca aaaatgctct aaatttttga aattctaaca aattccactc aactttatca
    40081 tcatctttga ctttcacaat atcttcctca aaataacatg gaataaagat agttttcttt
    40141 taaaacggtt gtaggaggaa aatctctcct acactattat tgattctgag agaaaattgc
    40201 aaaatatcat ctaccaaact ttcaattatt aagatttttt gcagtttatc tgaattagaa
    40261 tcgcaaggct tctcaataat ataattgtcg aattttttca ttctaacttc ctctccatta
    40321 tttcttcctt tatcaagaaa aagttacctc aatattaata aagtacaatt ctaaattaac
    40381 tccaattctt gattcatgat aatatttcag taactcatcg tgcaattaaa aacaaggatt
    40441 tttatttcag atgatccatc gtgaacactt catcagccat cttccaaatt gccggattat
    40501 gtgttgcgat aatgattagc ctattatcat ctcgaagaga tagcaaaatc tccataatca
    40561 actgagaggt tgctgggtct attgaagctg ttggctcatc tgccagaata aagggtggat
    40621 tctttaagat aatttttgcc aaggcaaccc gttgcgattc tccgcccgat aactcaaaga
    40681 tgcgcttatc taggtcaaga taagccaggc cgacctgttc taaagcctgc ttctgcctca
    40741 accgctgttc cgaccgactc aacttttgac caatgagacc tagcttaagg ttttcttcaa
    40801 tactttggtt ttcaattaag ccaaagttct ggaagaggta gcccaattcg tgacggaaaa
    40861 aatcactcga tttataattg gccaagtctt tacctcggta aaaaatcgtc ccatcataag
    40921 gttctaattt cccaatcatg ttcatcaagg ttgtttttcc gctaccactt gaaccaatta
    40981 aggcatagac ttttccagcc tcaaatgtca ttgaaagatt cgaaaataac tctcgttctc
    41041 caaaagattt actcacctgt ttaagttcaa tcatattaac ctcccttcaa aacaagaatg
    41101 gacatcttgt tttctttctg catttggaca tgtaactgta aaagagatag accagtaaag
    41161 agtaacaaga ctaagaaagc aactcctatc tctacctgaa gaaatacact cgcaacaaat
    41221 cccagtaaaa acacacccag ttgagcaaag agataagtgc gatggatttc taagaacctg
    41281 agacctgcaa tgcgtttgat aaaaatggca cgtctaaatt cttcaaagta gagcctattc
    41341 atagtgttaa acaacaagat tgaagttgca atcccaagca cagctcctgc tagcattacc
    41401 caacgttccc tctggatatt atccaataat gtgatgtagt tgtggtaacc tgtttgcatt
    41461 tctgagaccc aattttcaat gccttgtctc tggataagct cctgggcatc agacaattga
    41521 ttaaagagaa cgtagttctg tactgcgtct atccaaaaca aaatggactg tggaccagtt
    41581 gattggggtg ttataacaat gatgattgga tctttcaaaa actgctggta agaaataggg
    41641 gtcgtattat acacaaaacg atcctgacct gattctaaat aacctaccgt agcagtcatt
    41701 tgctgtcgtt catcttgact agacatgcga ctggttaagt cgtcttcaaa aacagattta
    41761 taatgttctt cctctgaacg gaggtgttca ggcagcaata agacaaactc ccctacatca
    41821 aggtgattca tcttttgctc aatagttgta tctacaggaa tgttttgacg ctccaagtat
    41881 tgcggtgtga cgataaggac attgccattc gggttgtaat cgtgccattc tgtagaggtt
    41941 atcaagtttt tggaggaagc catgccattt tgcaaagtac ggtcaattaa ctggtgtcta
    42001 gataagaaag ccttttgttc tgaaacagcc agatccatca attgatacca agttctgagt
    42061 ttcctttgag cttgttcatc aaaaccagga ctcgttcctt cacggctgat tgatagggta
    42121 atcagttgcc tttcctgact ccaagcctct tgtccaatcc gatgctgttg ccaggcttgg
    42181 gaatacttta agctactccc tattcccagc gttacaataa taatcgctaa tagttgacca
    42241 attaaaatca aactaatgat tcctctgaca ggcacttgcc cttttataat ctgcatcagg
    42301 tgtatctttt ttatcccaac tgcgaagagt tgagcgaaaa acagggagat cgacaacaag
    42361 atgagattat aactgagcaa gcctgctcct atggtcatta gggattgcgt tggaagcgac
    42421 agaatttttt gcataagaat ggcgagcacg ccagctaaac tgaaaccaac agctatccct
    42481 ttcaaatcct caccaactgg tctaaggaaa atggaccacc gtttctctcc tgaaatgagg
    42541 cgaatgcccg acgaccgtaa ttggctaatt tgactaatta aagtcagtgc tacaaaggtt
    42601 aagatgaaaa tcaatagact gattaactga aatccgttac taaagatagc cattaacgta
    42661 gatagtttag atggaattgt caggttcatg ttagtcaagc caagttgact tagctcctcc
    42721 cttagcaagt gaatatctag gtgtccatcg aatacaaaat agtttgtttc aacactacta
    42781 ctttgagcat cttctagttt tttttcctga agcccatcag gcaactttcc gtccccaaag
    42841 gtcgtataag aaaaaactgt agtaccttct gagttaggat cttggtgttg aatcgcaata
    42901 aagctatctg tttcttctgc tagtttttcc aagcgtgtag caacatgctg aaaagttgtt
    42961 tcaggggaag aatcatgtac aacaacgttg gagtaataat aggagacata tgtatcagtc
    43021 caaatggtaa atacccaaac aaagaacaaa ctagcaagca ggttggatat gagtataaat
    43081 aattttttca tagtgcattc cttattctaa aacagagggc agaaataccc ctgccctcta
    43141 taaactaaca agcttactaa tatctatctg gtattgttac aagctctcat cagaatacct
    43201 ttctctaaat agttgaattt taaagtaata cgaaatcaat cagccttccc aacatcaaga
    43261 ccattggact tcttttgcga ttgcactcga atctccaatt gtctattctt ctgaataaac
    43321 tgttggtaag gaattgtatt actaaacata aaaacaccta gtaatactaa tatagctaat
    43381 ttttgaattc cacgattttt cttcattcgt gaaacccttt tctttttcat tatccttatt
    43441 ttaacagata tattttattt agccaagcat ttttatgtaa tttttgactt tttttagttt
    43501 attaccataa agaattattt attcctttaa cttgctattt tgaactaaaa attttataat
    43561 gcaattaagc aaataggaag gtttattatc tttaatgaat acactcgcag agaaattcag
    43621 attgaaaaga aaagagttga gactctccca acaaactctt gcagaaggaa tttgtgaaca
    43681 aagtcagatt agtaaaattg agagagggca tttcattccc tccgcagacc ttttgttcaa
    43741 actctctcaa cgacttgaag taccattaga ttattttttt aatgaacaaa ttgaaattaa
    43801 atctaacctc tctaatttca agcaattgtc tgctcgacta ttagacgata gaaattatga
    43861 cgatttggaa tatatttata gaatagagat tgaacgaagt acttttctaa cactagaaga
    43921 ccgaacttac cttgaatgga ttaaagctat tattgacttc tatcaatatg acagtaagtg
    43981 tgaggcaatt tcttcattgg aaaatatatt attaaaagtc tcttcaaata ctctgattta
    44041 tttaaaggcg ttgaatactc tatctaattt ctattcctta gtgggtcgtg aacaagaata
    44101 tgaggcaaac tactctcatt taatagagtt gtatcagaca aaaaatttgg accatcaaga
    44161 gtttttattt ggctacatca gagttcgtta caactacgct cactacctag tatcaaagga
    44221 aaaatataat gaagccatcc aagaagctct tgaaacgatt gaactctgta aacaaagaca
    44281 gacaagctac caattggctc ctctacttat tcttgtagga aatgctggag ccaaatttct
    44341 agacagagaa caagtcaaaa attattatat agaagcaaga gagttatgta agatttataa
    44401 caatccttta atgttgatga agatagaaaa ttatttgaag gaactagata ctgtttagtt
    44461 aatcttgaca ttatagtttt tctgaaacgt taaataacct gtaaggctga tagttagatt
    44521 aatactatca gccttataat tatgtgactc tgagtccgtc tcgctccgtg aggtgcgaga
    44581 caaaacaacc acccgtttga cggattttca tgatttcaaa tttatattct tcgaaaatct
    44641 cttcaaacca cgtcagctct atctgcaatc tcaaaacagt gttttgagca acctgtggct
    44701 agctacctag tttgctcttt gattttcatt gagtattagt ctctagtgtt tgtttagcgt
    44761 cttaaagaga catttgagaa gattagaaac agttctctct tttctcctag tataatgatt
    44821 ggtaggaagg gagagagaag atgaaatcaa tgagaatctt atttttgtta gctttaattc
    44881 aaatcagttt gagtagctgt ttcctatgga aggaatgcat cttgtccttt aaacaaagta
    44941 cagctttttt catcggaagc atggttttcg tttcaggaat ctgtgctgga gtaaattatc
    45001 tttatacccg taagcaagaa gtccatagtg tcctagccag taagaagtcg gtgaagcttt
    45061 tttacagtat gttactctta attaatttgt taggagctgt tcttgttttg tcagataact
    45121 tgttcatcaa aaatacgctg cagcaagaat tagttgactt tttattgcca tccttctttt
    45181 tcctatttgg gctagatttg ctgatttttt tacccttgaa aaaatacgtg cgcgattttc
    45241 ttgctatgct ggacagaaaa aagacagtgt tggtgactat tttagcaaca cttcttttct
    45301 taagaaatcc aatgaccatt gtctcacttc tgatttatat tggactgggc ttgttttttg
    45361 cagcctatct tgtcccaaat tcggttaaga aggaagtttc cttttatggt catattttcc
    45421 gagatcttgt attggtcatt gttacgctca ttttctttta gagaaagcct gtttttgtat
    45481 ggatgttatg atgataagtt tagtaggaaa atagacacaa aagaaaagtt tttggtataa
    45541 taagagtatg tacacaaaaa atgaagaaga gttgcaagcc ttaggggagc gtttgggcca
    45601 tctattagca aagaatgatg ttttaatctt aactggagaa ctgggtgcag gtaaaacgac
    45661 ctttactaaa ggacttgcaa aaggattaca gatttctcaa atgattaaaa gtcccaccta
    45721 tactatcgtg agagagtatg aaggtcgact tccactttat cacctagatg tttatcgtat
    45781 tgaaggagat gctgattcta tcgacttgga tgagtttatc tttggtggcg gcgtgactgt
    45841 tattgagtgg ggaaatctct taggagatgc cttgccagat gcttatttag aattggaaat
    45901 tctaaaagaa gcagatggac gccgtttaaa ttttcaggca aagggtttgc gtgctgagaa
    45961 attgttagag gagcttcaat atggagtatg aattgctcat tagggaagca gagcccaaag
    46021 atgcagctga attagtgacc tttttaaatc gtgtgagttt ggagacagac tttaccagcc
    46081 tagacggaga tggtattctc ttgactagtg aggagatgga aatattcctc aacaagcaag
    46141 ctagttcgga caatcagata accttacttg cattcttaaa tggcaaaatt gctggtattg
    46201 taaatattac agctgatcag cgtaagagag tccgtcatat tggagatctc ttcattgtga
    46261 ttggaaaaag atattggaat aatggcttgg gaagtttgtt actagaagaa gcgatagagt
    46321 gggcacaagc aagtggcatt ctgcgtcgtc tccaactgac tgtccaaact cgtaatcaag
    46381 cagcagtcca tctttatcaa aagcatggct ttgtcattga aggtagccaa gagcgtgggg
    46441 catatataga agaagggaaa tttatcgatg tttacttgat gggtaaactg ataggttagt
    46501 agaaatatgg ttaaaaaaat tattggaatg gtgctagctt tactttctgt aactgtagta
    46561 ggagtaggtg tttttgctta tactatttat caacaaggga cagaaacctt agctaaaacc
    46621 tataaaaaaa tcggcgaaga aaccaaggtt attgaagcga ctgaacctct aaccattctg
    46681 ttaatgggag tggacaccgg aaatgctgaa cgaactgaaa cttgggtcgg tagaagtgat
    46741 agcatgatct tgatgacagt gaatcctaaa acgaaaaaaa caacaatgat gagtttagag
    46801 cgggatattc tgacgcgcat tgaatcaggg aatggtcagg ctcatgaagc gaaactgaac
    46861 tcagcatatg cagatggtgg agcagagctt gctatagaaa ccattcaaaa aatgatgaat
    46921 atccatattg atcgctatgt aatggtcaat atgagagggt tgcaaaaact agtggatgca
    46981 gtaggaggta ttacagtcaa taatatccta ggtttcccaa tttctatcag tgaccaagaa
    47041 gaatttaata ctatttctat cggtgttggg gagcaacata ttgggggaga agaagcccta
    47101 gtctatgcac gaatgcgtta ccaagatcct gagggggatt atggtcgtca aaaacgtcaa
    47161 cgtgaagtta ttcaaaaagt catggaaaaa gctctcagtt taaatagcgt tggtcattat
    47221 caagagattc taaaagcttt gagtgacaat atgcagacca atattgattt gtctgcaaaa
    47281 agtatcccta acttgctagg ctataaagat tcatttaaaa ccattgaaac tcagcagttg
    47341 cagggtgaag gagagatact tcaaggtgtt tcttaccaga ttgtttcgag agcacatatg
    47401 ttggaaatgc aaaatatact ccgacgttct ttgggacaag aagaagttac tcagcttgaa
    47461 accaatgcgg ttttatttga agatttattt ggcagagcac ctgttggtga tgaagataat
    47521 taacttttga tacaaataaa aaaatcaatc gtaggaaaat tcctgcgatt ttttcaaaaa
    47581 aatacgaata gataggtagg aggaaacatg aaagcagaaa tcattgctgt tggaacagag
    47641 attttgacag gacagattgt caacaccaat gcccagtttt tgtcggaaaa actagctgag
    47701 attggggtag atgtatattt tcagacggct gtaggagaca atgaagttcg tctcttgtct
    47761 ttgcttgaga ttgccagtca acgtagcagt ctggtgattt tgacaggcgg tttggggcca
    47821 actgaggacg acctaaccaa acaaacccta gctaaatttt tagggaaagc attagtcttt
    47881 gatcctcagg ctcaggagaa gttggatatc ttttttgccc tgcgaccaga ctatgcccga
    47941 acaccgaata acgaaagaca agctcaaatt gtagaaggag cgattccact gccaaacgaa
    48001 acaggactgg ctgttggagg aaaattagaa gtagacggag tgacctatgt cgtccttcca
    48061 ggtccgccaa gtgaattgaa acccatggtc ttaaaccaac ttctacccaa gttgatgaca
    48121 gggagcaagc tgtattcccg agttcttcgt ttctttggga ttggcgagag ccagctggtt
    48181 acgattttgg ctgatttaat tgataatcag atcgatccta ccttggcccc ttatgccaag
    48241 acaggagaag tcactctacg tctgtcaaca aaggctagca gtcaagaaga ggcgaatcaa
    48301 gcgctggata tcttggaaaa tcaaatcttg gactgccaga ctttcgaagg aatttcttta
    48361 cgagactttt gctatggtta tggggaagaa actagtttag ccagcattgt ggtagaagaa
    48421 ctgaaaaggc aagggaaaac catcgcggct gcagagagtt tgacggcagg tcttttccaa
    48481 gctaccgtgg cgaatttttc tggagtttca agtatattta agggtggttt tgtgacctat
    48541 agcttggagg aaaaatcaag gatgttggat attcctgcca agaatttgga agaacatggt
    48601 gtggtgtctg aatttacagc tcagaagatg gctgagcagg cacgaagcaa gacccagtct
    48661 gattttggaa ttagtttgac tggagtggca ggaccagata gcctagaagg acacccagtt
    48721 gggacagtct tcataggctt ggcgcaagat caaggaactg aggttatcaa ggttaatatt
    48781 ggaggcagaa gccgagcaga tgtacgtcac attgcggtta tgcatgcctt taacctagtt
    48841 cgcaaggctt tattaagtga ctaacttttg atataatagt agataggtct gaggatcatt
    48901 agaatgtagg agaatagaat ggcgaaaaaa ccaaaaaaat tagaagaaat ttcaaaaaaa
    48961 tttggggcag aacgtgaaaa ggccttgaat gacgctctta aattgattga gaaagacttt
    49021 ggtaaaggat caatcatgcg tttgggtgaa cgtgcggagc aaaaggtgca agtgatgagc
    49081 tcaggttctt tagctcttga cattgccctt ggctcaggtg gttatcctaa gggacgtatc
    49141 atcgaaatct atggcccaga gtcatctggt aagacaacgg ttgcccttca tgcagttgca
    49201 caagcgcaaa aagaaggtgg gattgctgcc tttatcgatg cggaacatgc ccttgatcca
    49261 gcttatgctg cggcccttgg tgtcaatatt gacgaattgc tcttgtctca accagactca
    49321 ggagagcaag gtcttgaaat tgcgggaaaa ttgattgact caggtgcagt tgatcttgtc
    49381 gtagtcgact cagttgctgc ccttgttcct cgtgcggaaa ttgatggaga tatcggagat
    49441 agccatgttg gtttgcaggc tcgtatgatg agccaggcca tgcgtaaact tggcgcctct
    49501 atcaataaaa ccaaaacaat tgccattttt atcaaccaat tgcgtgaaaa agttggagtg
    49561 atgtttggaa atccagaaac aacaccgggc ggacgtgctt tgaaattcta tgcttcagtc
    49621 cgcttggatg ttcgtggtaa tacacaaatt aagggaactg gtgatcaaaa agaaaccaat
    49681 gtcggtaaag aaactaagat taaggttgta aaaaataagg tagctccacc gtttaaggaa
    49741 gccgtagttg aaattatgta cggagaagga atttctaaga ctggtgagct tttgaagatt
    49801 gcaagcgatt tggatattat caaaaaagca ggggcttggt attcttacaa agatgaaaaa
    49861 attgggcaag gttctgagaa tgctaagaaa tacttggcag agcacccaga aatctttgat
    49921 gaaattgata agcaagtccg ttctaaattt ggcttgattg atggagaaga agtttcagaa
    49981 caagatactg aaaacaaaaa agatgagcca aagaaagaag aagcagtgaa tgaagaagtt
    50041 acgcttgact taggcgatga acttgaaatc gaaattgaag aataagctgt taaagcagtg
    50101 gagaaatccg ctactttttc gatttttgat tcaagttttt agattatata tagtagcttg
    50161 aaataagata tgaacaactt tattaggaaa gtcaaattaa tttctagaaa tgttttagca
    50221 gctacagcgt actattccaa actcaaccaa ctataataga tcgaaactag aatagtacac
    50281 atctacttct aaaacattgt taaaaatcga tttgactgtc cttatttcat tccgctatat
    50341 ataagtttgc tgtttcttgt cgctcctctg gaaagctgat ataatagctt tatgaataaa
    50401 aaacgaacag tggacctgat acatggtccg attcttccct cgctcttaag cttcaccttt
    50461 ccaatcttgc tatcaaatat ttttcaacag ctctataaca ctgctgatgt cttgattgtt
    50521 ggacgatttc ttggtcaaga atccttggct gcagtaggag cgacgacagc gatttttgac
    50581 ctgattgtag gttttacact tggtgttggc aatggcatgg gaattgtcat tgctcgttat
    50641 tatggggctc ggaatttcac taaaatcaag gaagcagtag cagccacctg gattttaggt
    50701 gctcttttga gcattctagt tatgttgctg ggctttcttg gcttgtatcc tctcttgcaa
    50761 tacttagata ctcctgcaga aattcttcct caatcttatc aatatatttc tatgattgtg
    50821 acctgtgtag gtgtcagctt tgcttataat ctttttgcag gcttgttgcg gtctattggt
    50881 gacagtctag cagccctggg atttctgatt ttctctgcct tggttaatgt ggttctggat
    50941 ctctatttta ttacgcaatt gcatctggga gttcaatccg caggacttgc taccattatt
    51001 tcgcaaggtt tatcagcggt tctctgcttt tattatattc gtaaaagtgt gccagaactc
    51061 ttgccacagt ttaaacattt caaatgggac aaaagcttgt acgcggatct cttggagcaa
    51121 ggtttggcta tgggcttgat gagttcaatt gtatctatcg gcagtgtgat tttacagttt
    51181 tctgttaata catttggtgc agtgatcatt agtgcccaga cggcagctcg acgcattatg
    51241 acctttgccc ttcttcctat gaccgctatt tctgcatcaa tgacgacctt tgcttctcag
    51301 aatctaggag ctaagcgacc tgaccgcatt gttcaaggtc ttcgaatcgg cagtcgttta
    51361 agtatatcct gggcagtttt tgtttgtatt ttcctctttt ttgccagtcc agccttggtt
    51421 tccttcttgg ctagttcgac agatggttac ttgatagaaa atggaagtct ctatctgcaa
    51481 atcagttcag ccttttatcc cattttgagc ctcttgttga tttatcgcaa ttgcttgcag
    51541 ggcttggggc agaagatcct tcctctagtt tctagcttta ttgaactaat cggaaaaatc
    51601 gtttttgtgg ttttgattat tccttgggca ggatataagg gtgttatcct ttgtgaacct
    51661 cttatctggg ttgccatgac agttcaactg tacttctcac tattccgtca tcccttgata
    51721 aaagaaggca aggcaatctt ggcaactaaa gtgcaatcct agttggattt actgaataaa
    51781 atccatttcc tctagtgaaa atcgaaaaaa cttgtgttct cttctttagt ttggtgttga
    51841 aaatagttta acagactttt gacttctttt atatgatata ataaagtata gtatttatga
    51901 aaaggacata tagagactgt aaaaatatac ttttgaaaag ctttttagtc tggggtgtta
    51961 ttgtagatag aatgcagacc ttgtcagtcc tatttacagt gtcaaaatag tgcgttttga
    52021 ggttctatct acaagcctaa tcgtgactaa gattgtcttc tttgtaaggt agaaataaag
    52081 gagtttctgg ttctggattg taaaaaatga gttgttttaa ttgataagga gtagaatatg
    52141 gaaattaatg tgagtaaatt aagaacagat ttgcctcaag tcggcgtgca accatatagg
    52201 caagtacacg cacactcaac tgggaatccg cattcaaccg tacagaatga agcggattat
    52261 cactggcgga aagacccaga attaggtttt ttctcgcaca ttgttgggaa cggttgcatc
    52321 atgcaggtag gacctgttga taatggtgcc tgggacgttg ggggcggttg gaatgctgag
    52381 acctatgcag cggttgaact gattgaaagc cattcaacca aagaagagtt catgacggac
    52441 taccgccttt atatcgaact cttacgcaat ctagcagatg aagcaggttt gccgaaaacg
    52501 cttgatacag ggagtttagc tggaattaaa acgcacgagt attgcacgaa taaccaacca
    52561 aacaaccact cagaccacgt tgacccttat ccatatcttg ctaaatgggg cattagccgt
    52621 gagcagttta agcatgatat tgagaacggc ttgacgattg aaacaggctg gcagaagaat
    52681 gacactggct actggtacgt acattcagac ggctcttatc caaaagacaa gtttgagaaa
    52741 atcaatggca cttggtacta ctttgacagt tcaggctata tgcttgcaga ccgctggagg
    52801 aagcacacag acggcaactg gtactggttc gacaactcag gcgaaatggc tacaggctgg
    52861 aagaaaatcg ctgataagtg gtactatttc aacgaagaag gtgccatgaa gacaggctgg
    52921 gtcaagtaca aggacacttg gtactactta gacgctaaag aaggcgccat ggtatcaaat
    52981 gcctttatcc agtcagcgga cggaacaggc tggtactacc tcaaaccaga cggaacactg
    53041 gcagacaagc cagaattcac agtagagcca gatggcttga ttacagtaaa ataataatgg
    53101 aatgtctttc aaatcagaac agcgcatatt attaggtctt gaaaaagctt aatagtatgc
    53161 gttttcttgt ggagatattt ccttcaatgt ttgctactat attaaacaaa aatcagaaag
    53221 caaactagaa agttatgctc aaataaaatc taaatttgac aatgtaaacc gagtcggata
    53281 gctttaagta ctgttttgag gttgaagata cgatttttga taggaactca tcaaataggt
    53341 ttaatatcat caatcttaga tttttaagca gcatcaataa attgcttcct tgttttgtca
    53401 taattttttt atttaaaaaa ttatgacaag agtgtgctat tctttttatg agaggtgtat
    53461 aaatatgata aatgtatgtg atgttggaaa aagaataaaa gaacttagaa tatcttcaaa
    53521 tcttactcaa gataagattg ctgagtattt gtctttgaat caaagcatga ttgccaaaat
    53581 ggaaaaaggt gaaaggaata tcacgaatgg atttaagtaa taaagcttca aatcttagaa
    53641 aaaagttggg agctgatggt gaatcgccga tagatatttt taaattggta caaaagatag
    53701 aaaatttgac gctggtattt tatggactcg gaaagaatat tagcggagtc tgttataaag
    53761 gaactcagtt cagtctcatt gcagtcaatt cagacatgcc attaggaagg taaagatttt
    53821 ctttagcaca tggactgtat catctttatt atgatgaggt gaagaagagt tcagtcagtc
    53881 ttatcttgat tggtgaagga gatgaaactg aaagaaaagc ggatcagttt gcttcttatt
    53941 ttttaatttt cccatcttca ctgtatagga tggttgagga aatcagagaa aatgccaata
    54001 gaactcatct tgaagtagaa gatattataa aattgggtca gttttatggt atcagtcata
    54061 aagctatgtt atatagattg aggaatgatg gataccttga tgcagaagaa attaaaaata
    54121 tggatattag tgttatagag acagcttcaa gattaggcta tgatacaagt ttatatcgtc
    54181 ctttgtcaga aagtaaaaaa gaaatggcat taggataata tattaattca actgaacaac
    54241 ttttagaaaa taacagaatt tcgcaaggga agtatgagga actgttacta gatgctttca
    54301 gatatgatat tgtatatggg ctagatgaag aggggggagt tgtcgtttga ctagtcgtgt
    54361 atttattgat gcagattgta tttcagtatt tttatgggtt ggcactgaac atcttttaga
    54421 aaagctctat ttgggtaaaa ttgttattcc acaagaggtg tatgatgaaa tcaatatacc
    54481 tacaattccc catttaaaat ctaggataga tcagttggta gctaagggtt cagctgagat
    54541 tgtgagcata gacattggaa ctgaagaata cgcattatat agagatttaa caagaaatca
    54601 tgatagtaac aagattattg gtaagggaga aggggcatct atttccttag cgaaaaagca
    54661 taatgggata ttaggaagta ataacctaag agatgttaaa tcatatgtag aagaattttc
    54721 tttagaatat atgacaacag gagatatact gattgaagcg tttaaagcgt aatttattac
    54781 tgaataagag ggcaatcata tctggaataa tatgcttaaa aagagaagga aaattggtgc
    54841 aaattcattt tcagactatc ttcgtggaag tattcatcaa aatagacaaa aataaatttg
    54901 gataaatcga actcactatt caggaggcat atgagcaatt cgaaaaagaa aagtgtcaaa
    54961 ttgagcctat aggagtagaa gtgaaatagt aagtcctgca tagtggatga gagaaaagtt
    55021 ctccttgaag ttttcctgaa ctatcagtcg catgtcaaac gatatgtagg gtaatgtgag
    55081 aggggatagc gagtagtttt tggttatttt atcaaaaaac ttatatttta ttataccgaa
    55141 tgataaaata taataaaaat gatagaataa ggaaaaaaca tgaatgtcaa aaagataatg
    55201 tcaatttttc aatcctttta tgttgatgtc agtattgagg aactgacttt gactttacca
    55261 atcagttttg taaaaaggtt tgagtatact caaatgactt ttcataagga atcattttta
    55321 ttgattaaag aaaagagaag ggggagtttg agttcatttg ttactcaggc tcgcactatg
    55381 ggtgaaaaag ccaatatgga tgttgttttg gtgttttcga agttatcaga cagtgaaaaa
    55441 aagcaattac ttcaagctag agttccgttt gtagacttta agggaaacct cttcttccct
    55501 ccattgggac tagtactcaa tgcgaatgat actgaagtcc ctaaggaatt aacacctagc
    55561 gaacaattaa cgtggattgc ctttttattg acaaaaggtc aaaaagtagt agatgttgat
    55621 ttgctttcac aagtcactgg acttccaaac tcaacaattt ataggtgttt gaggactttt
    55681 aaagctttat attggttaaa caagcaaaat aagctttaca catatacggt gtcaaagaaa
    55741 gaattattct taaaatccgt gtcatgttta tttaatccca tcaaaaaacg gattttattg
    55801 ccagatggcg atataaagca gataaaatct gtttctaacc ttctatatgg tggtgcttat
    55861 gctttgtcgc attcaacttt tttagctgaa acggatgaaa atattagcta tgtcatatgg
    55921 cagagaaaat tcaatcagtt atccttgcca ctttctcagc atgttttaaa atgaaagatg
    55981 ctagagatat ggaaatatcg tccttttgta tctgagtttt ggaatgattt taaaaataat
    56041 catgataaac aatttgtaga tccgatttct ctttatttga ccttaaaaga tgatgatgac
    56101 ccacgtatag aggaagagag tgaagcacta gaaaatatga tattacagta tctgggagaa
    56161 gatgatgcca gctaatacga aagttatttt tcaagaaatg tttgcggatt ttcagaacta
    56221 ttatgttctg attgggggaa ctgctacctc tatcgtattg gattcgcaag gatttaaaag
    56281 tcgcacaaca aaagattatg atatggtcat cattgatgaa gtaaaaaata aggaatttta
    56341 tactaccttg aatcattttt tagaattggg agagtatcaa ggaagtcaga aagatgagaa
    56401 agcgcagctt tttcgattta caacaactaa tcctgagttt ccttctatga ttgaactatt
    56461 tagtatctta ccagaatatc cattaaagaa ggacggtcga gaaattccct tacattttga
    56521 ccaagatgct agtttatcag ccttattatt ggatgaagat tattataata tattggtgca
    56581 tgaaaaagaa accattcagg ggtattcggt attgagtaat tgtggtt
//
LOCUS       DAVLVP010000005        53190 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.005, whole genome shotgun sequence.
ACCESSION   DAVLVP010000005 DAVLVP010000000
VERSION     DAVLVP010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 53190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53190
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.005"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            1..236
                     /locus_tag="VYN69_000264"
                     /pseudo
     CDS             1..236
                     /locus_tag="VYN69_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            651..1268
                     /locus_tag="VYN69_000265"
     CDS             651..1268
                     /locus_tag="VYN69_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEV1532619.1"
                     /translation="MSQDKQMKAVSPLLQRVINISSIVGGVGSLIFCIWAYQAGILQS
                     KETLSAFIQQAGIWGPPLFIFLQILQTVVPIIPGALTSVAGVFIYGHIIGTIYNYIGI
                     VIGCAIIFYLVRLYGAAFVQSVVSKRTYDKYIGWLDKGNRFDRFFIFMMIWPISPADF
                     LCMLAALTKMSFKRYMTIIILTKPFTLVVYTYGLTYIIDFFWQML"
     gene            complement(1588..2475)
                     /locus_tag="VYN69_000266"
     CDS             complement(1588..2475)
                     /locus_tag="VYN69_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1532620.1"
                     /translation="MTKLYGSLEAGGTKFVCAVGDENFNVVEKTQFPTTTPIETIDKT
                     IEFFSKFDNLAGLAVGSFGPIDIDKNSKTYGFITTTPKPNWANVDLLGALRRALNVPM
                     YFTTDVNSSAYGEMVARNNAGGRIENLVYYTIGTGIGAGVIQRGEFIGGVGHPEMGHY
                     YVARHPMDIEKEFKGVCPFHKGCLEGYAAGPSLEARTGVRGETIELNNPVWDVQAYYI
                     AQAAVNATVTFRPDVIVFGGGVMAQQHMLDRVREKFTSLLNGYLPVPDVRDYIVTPAV
                     AGNGSATLGNFVLAKEVSK"
     gene            complement(2624..4507)
                     /locus_tag="VYN69_000267"
     CDS             complement(2624..4507)
                     /locus_tag="VYN69_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1532621.1"
                     /translation="MNNQEIAKKVIDALGGRENVNSVAHCATRLRVMVKDEEKINKEV
                     IENLEKVQGAFFNSGQYQIIFGTGTVNKMYDEVVVLGLPTSSKDDMKAEVAKQGNWFQ
                     RAIRTFGDVFVPIIPVIVATGLFMGVRGLFNALEMPLPGDFATYTQILTDTAFIILPG
                     LVVWSTFRVFGGNPAVGIVLGMMLVSGSLPNAWAVAQGGEVTAMNFFGFIPVVGLQGS
                     VLPAFIIGVVGAKFEKAVRKVVPDVIDLLVTPFVTLLVMSILGLFVIGPVFHVVENYI
                     LIATKAILSMPFGLGGFLIGGVHQLIVVSGVHHIFNLLEVQLLAADHANPFNAIITAA
                     MTAQGAATVAVGVKTKNPKLKTLAFPAALSAFLGITEPAIFGVNLRFRKPFFLSLIAG
                     AIGGGLASILGLAGTGNGITIIPGTMLYVGNGQLPQYLLMVAVSFALGFALTYMFGYE
                     DEVDATAAAKQAEVAEEKEEVAPAALQNETLVTPIVGDVVALADVNDPVFSSGAMGQG
                     IAVKPSQGVVYAPADAEVSIAFPTGHAFGLKTRNGAEVLIHVGIDTVSMNGDGFEAKV
                     AQGNKVKAGDVLGTFDSNKIAAAGLDDTTMVIVTNTADYASVAPVATGSVAKGNAVIE
                     VKI"
     gene            4884..6338
                     /locus_tag="VYN69_000268"
     CDS             4884..6338
                     /locus_tag="VYN69_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1532622.1"
                     /translation="MEWTTERRYRLYQDWTQEEIQHIKENMAQSPWHTHYHVEPKTGL
                     LNDPNGFSYFDGKWILFYQNFPFGAAHGLKSWAQLESDDLVHFKETGIKVLPDTPLDS
                     HGAYSGSAMQFGDNLFLFYTGNVRDENWIRHPYQIGALMDKEGKITKIDKILIDQPAD
                     STDHFRDPQIFNFKGQYYAVVGGQDLEKKGFVRLYKAVNNDYTNWQAVGDLDFANDRT
                     AYMMECPNLVFVEEQPVLLYCPQGLDKKVLDYDNIFPNMYKIGASFDPKNAKMVDVSQ
                     LQNMDYGFEAYATQAFNAPDGRALAVSWLGLPDVSYPSDRFDHQGTFSLVKELTIKDD
                     KLYQYPVAAIKDLRASEEAFSNRSQTKNTYELELNLEANSQSEIVLLADKEGKGLSIN
                     FDLVNGQVTVDRSQAGEQYAQEFGTTRSCPIENQATTATIFIDNSVFEIFINKGEKVF
                     SGRVFPHADQNGILIKSGNPTGTYYELDYGRKTN"
     gene            6319..7284
                     /locus_tag="VYN69_000269"
     CDS             6319..7284
                     /locus_tag="VYN69_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002929307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1532623.1"
                     /translation="MVAKLTDVAKLAGVSPTTVSRVINKKGYLSEKTIQKVNEAMREL
                     GYKPNNLARSLQGKSAKLIGLIFPNISNVFYAELIDKLEHQLFKNGYKTIICNSEHDS
                     EKEREYIEMLEANQVDGIISGSHNLGIEDYNRVTAPIISFDRNLSPDIPVVSSDNYAG
                     GVLAAQTLVKTGAQSIIMITGNDNSNSPTGLRHAGFASVLPKAPIINVSSDFSPVRKE
                     MEIKNILTREKPDAIFASDDLTAILVIKIAQELGISVPKELKVIGYDGTYFIENYYPQ
                     LATIKQPLEEIACLTIDLLLQKIEGKEVATTGYFLPVTLLPGKSI"
     gene            complement(7320..8594)
                     /locus_tag="VYN69_000270"
     CDS             complement(7320..8594)
                     /locus_tag="VYN69_000270"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEV1532624.1"
                     /translation="MKISWNGFSKKSYQERLELLKAQALLSPERQASLEKDEQMSVTV
                     ADQLSENVVGTFSLPYSLVPEVLVNGQEYTVPYVTEEPSVVAAASYASKIIKRAGGFT
                     AQVHQRQMIGQVALYQVANPKLAQEKIASKKAELLELANQAYPSIVKRGGGARDLHVE
                     QIKGEPDFLVVYIHVDTQEAMGANMLNTMLEALKPVLEELSQGQSLMGILSNYATDSL
                     VTASCRIAFRYLSRQKDQGREIAEKIALASQFAQADPYRAATHNKGIFNGIDAILIAT
                     GNDWRAIEAGAHAFASRDGRYQGLSCWTLNLEREELVGEMTLPMPVATKGGSIGLNPR
                     VALSHDLLGNPSARELAQIIVSIGLAQNFAALKALVSTGIQQGHMKLQAKSLALLAGA
                     SESEVAPLVEQLIADKTFNLETAQRYLENLRS"
     gene            complement(8594..9766)
                     /locus_tag="VYN69_000271"
     CDS             complement(8594..9766)
                     /locus_tag="VYN69_000271"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004181031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEV1532625.1"
                     /translation="MTIGIDKIGFATSQYVLKLQDLAEARGIDPEKLSKGLLLKELSI
                     APLTEDIVTLAASASDSILTEQERQEVDMVIVATESGIDQSKAAAVFVHGLLGIQPFA
                     RSFEIKEACYGATAALHYAKLHVENSPESKVLVIASDIAKYGIETPGEPTQGAGSVAM
                     LITQNPRMMAFNNDNVAQTRDIMDFWRPNYSTTPYVNGVYSTQQYLDSLKTTWLEYQK
                     RYQLTLDDFAAVCFHLPYPKLALKGLKKIMDKSLPQEKKDLLQKHFDQSILYSQKVGN
                     IYTGSLFLGLLSLLENTDSLKAGDKIALYSYGSGAVAEFFSGELVEGYEAYLDKDRLN
                     KLNQRTVLSVADYEKVFFEEVNLDETNSAQFAGYENQDFALVEILDHQRRYSKVEK"
     gene            complement(9897..10145)
                     /locus_tag="VYN69_000272"
     CDS             complement(9897..10145)
                     /locus_tag="VYN69_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532626.1"
                     /translation="MVSSSGSEFQSGWQEHQLIAEKVSKTLYKTFDKDVREIPTSQFY
                     QKFVDEMGRTYSGNLILQELITVNGAYKATYIGELSSN"
     gene            complement(10213..10838)
                     /locus_tag="VYN69_000273"
                     /pseudo
     CDS             complement(10213..10838)
                     /locus_tag="VYN69_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            complement(10900..11349)
                     /locus_tag="VYN69_000274"
     CDS             complement(10900..11349)
                     /locus_tag="VYN69_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1532627.1"
                     /translation="MTLTTFLLQAVASFLAIITFLIVLNVQRSMLLPGGILGMTVWLI
                     YLLLKEPTNVIVATFIAAIIGSCVSQILSILYKTPAVVFILAILAPLVPGYLSYRTTA
                     FFVTGDYNKALASATLVVMLALVISIGMASGTVILRLYHYIKTHRVS"
     gene            complement(11351..12109)
                     /locus_tag="VYN69_000275"
     CDS             complement(11351..12109)
                     /locus_tag="VYN69_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEV1532628.1"
                     /translation="MEESKELNAVIDVIMLAGTILLKSGSEIHRVEDTMIRIAHSQGI
                     VDCNVLAMPAAIFFSIENTNISRMKRVTSSSYNIEKVCDVNQISRQLVGGQIDLETAF
                     KQLTALQAQPLPYTKLQVTLAATFSAPFFSVMFSGNIYDALGAGVATLFGFAFSLYVE
                     KFIRIPFVTAFAGAFVFGIIAQFWARYTGFPSTADLIIAGAVMPFVPGIALTNAVRDI
                     MTNHINSGMSKMFESLLITLALGAGTSVALVLMN"
     gene            complement(12224..14203)
                     /gene="pknB"
                     /locus_tag="VYN69_000276"
     CDS             complement(12224..14203)
                     /gene="pknB"
                     /locus_tag="VYN69_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEV1532629.1"
                     /translation="MIQIGKIFAGRYRIVKQIGRGGMADVYLAKDLILDGEEVAVKVL
                     RTNYQTDPIAVARFQREARAMADLDHPHIVRITDIGEEDGQQYLAMEYVAGLDLKRYI
                     KEHYPLSNEEAVRIMGQILLAMRLAHTRGIVHRDLKPQNILLTPDGTAKVTDFGIAVA
                     FAETSLTQTNSMLGSVHYLSPEQARGSKATVQSDIYAMGIIFYEMLTGHIPYDGDSAV
                     TIALQHFQKPLPSVIAENPSVPQALENVIIKATAKKLTNRYRSVSEMYVDLSSSLSYN
                     RRNESKLIFDETSKADTKTLPKVSQSTLTSIPKVQAQTEHKSIKNPSQAVTEETYQPQ
                     APKKHRFKMRYLILLASLVLVAASLIWILSRTPATIAIPDVAGQTVAEAKATLKKANF
                     EIGEEKTEASEKVEEGRIIRTDPGAGTGRKEGTKINLVVSSGKQSFQISNYVGRKSSD
                     VIAELKEKKVPDNLIKIEEEESNESEAGTVLKQSLPEGTTYDLSKATQIVLTVAKKAT
                     TIQLGNYIGRNSTEVISELKQKKVPENLIKIEEEESSESEPGTIMKQSPGAGTTYDVS
                     KPTQIVLTVAKKVTSVAMPSYIGSSLEFTKNNLIQIVGIKEANIEVVEVTTAPAGSVE
                     GMVVEQSPRAGEKVDLNKTRVKISIYKPKTTSATP"
     gene            complement(14200..14940)
                     /locus_tag="VYN69_000277"
     CDS             complement(14200..14940)
                     /locus_tag="VYN69_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEV1532630.1"
                     /translation="MEISLLTDVGQKRTNNQDYVNHYVNRAGRTMIILADGMGGHRAG
                     NIASEMAVTDLGVAWVDTQIDTVNEVREWFAHYLEIENQKIHQLGQDEAYRGMGTTLE
                     VLAIIDNQAIYAHIGDSRIGLIRGEEYHQLTSDHSLVNELLKAGQLTPEEAEAHPQKN
                     IITQSIGQKDEIQPDFGTVILESGDYLLLNSDGLTNMISGSEIRDIVTSDIPLADKTE
                     TLVRFANNAGGLDNITVALVSINEEDAE"
     gene            complement(14955..16268)
                     /gene="rsmB"
                     /locus_tag="VYN69_000278"
     CDS             complement(14955..16268)
                     /gene="rsmB"
                     /locus_tag="VYN69_000278"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEV1532631.1"
                     /translation="MTKVETARSLALAVLEDVFVNQAYSNIALNKHLKGSQLSAADKG
                     LVTELVYGTVARKLTLEWYLSHFIEDRDQLDSWLYVLLLMSAYQLRYLDKIPDHAVVN
                     EAVELAKLRKKGSEKLVNAVLRRILREGWPDIASIKRKNKRDSIAYSLPVWLVAKLKE
                     EYGEERAKAIFESLLVRNKASIRVTDLSRKEEIQALLEANDSLLATTGLVKEQGHFAG
                     HNLFADGAITIQDESSQLVAPTLDLQGDERVLDACAAPGGKTAHIASYLTTGQVTALD
                     LYDHKLDLIQENAQRLGVEDRVQTQKLDARKVHEFFDQDSFDKILVDAPCSGIGLLRR
                     KPDIKYNKETADFASLQEIQLEILGSVCQILGKGGIITYSTCTIVSEENFQVVKAFLE
                     SHPEFEQVKLEHECKDIMKDGCILITPELYGSDGFFISQFRKISD"
     gene            complement(16261..17196)
                     /locus_tag="VYN69_000279"
     CDS             complement(16261..17196)
                     /locus_tag="VYN69_000279"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEV1532632.1"
                     /translation="MTKLIFMGTPDFSATVLKGLLTDDRYEILAVVTQPDRAVGRKKV
                     IQETPVKQAAKEAGLSIYQPEKLSGSPEMEDLMKLGADGIVTAAFGQFLPSKLLDSMD
                     FAVNVHASLLPRHRGGAPIHYALIQGDEEAGVTIMEMVKEMDAGDMISRRSIPITDED
                     NVGTLFEKLALVGCDLLLDTLPAYIAGDIKPEPQDTSQVTFSPNIKPKEEKLDWNKTN
                     RQLFNQIRGMNPWPVAHTFLKGDRFKIYEALPVEGQGNPGEILSIGKKELIVATAEGA
                     LSLKQVQPAGKPKMDIASFLNGVGRTLTVGERFGD"
     gene            complement(17209..19605)
                     /locus_tag="VYN69_000280"
     CDS             complement(17209..19605)
                     /locus_tag="VYN69_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001162119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEV1532633.1"
                     /translation="MALAKIIVDVPLMQTDQPYSYRIPEEFEGMLEVGMRVHVPFGKG
                     NRLIQGIVLGLKSQSDGEEMEQDLKDIAEVLDFSPVLTPEQLWLAEELRKSVFSYKIS
                     ILKAMLPGFLNSSYDKILYPLEGLSQEERVRLFGSEDSLAFSSLDLAKQAEMMRLTRK
                     GLLGLEYQAVDQKKVKTQSWYEVDHAQLEGVEISTRAKKKLELRDYLLSHPESASLAS
                     LLESYSREQVNFFVDQGAVTIVQKEVQRSAAYFEGIEASRPLELNPEQRQARDAVVSS
                     IGSSQPPFLLQGITGSGKTEVYLQIIQGALDKGKTAILLVPEISLTPQMTERFIARFG
                     DKVAILHSGLSNGEKYDEWRKVERGDAQVVVGARSAIFAPLKNLGVMIIDEEHEAAYK
                     QDSNPRYHAREVAILRAQYNQATLVLGSATPSLESRARAGKGVYQHLRLTQRANPLAT
                     IPEVQVIDFRDYIGQNETSNFTPPLLEAIQDRLVKKEQVVLMLNRRGYSSFVMCRECG
                     TVDTCPNCDISLTLHMDTKTMNCHYCGFSKDIPQVCPNCKSRSIRYYGTGTQKAYDEL
                     AELFPQARILRMDVDTTRKKGSHQALLDQFGRGEADILLGTQMIAKGLDFPNVTLVGV
                     LNADTALNLPDFRSSERTFQLLTQVAGRAGRAEKAGQVLIQSYNPQHYAIRFAKDQDY
                     EGFYAYEMGIRRQLGYPPYYFTIGITLSHKKEEEVFKRAYEVMNILRSGLSETSPILG
                     PTPKPIARTHNLYHYQILIKYRLEDELGPTLNQVLALTQERENSELRLSIDHEPQQFL
                     "
     gene            complement(19671..19985)
                     /locus_tag="VYN69_000281"
     CDS             complement(19671..19985)
                     /locus_tag="VYN69_000281"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEV1532634.1"
                     /translation="MMLKPSIDTLLDKVPSKYSLVILEAKRAHELEAGAPATQGFKSE
                     KSTLRALEEIESGNVTIHPDPEGKREAVRRRIEEEKRRKEEEEKKIKEQIAKEKEDGE
                     KI"
     gene            complement(20010..20636)
                     /gene="gmk"
                     /locus_tag="VYN69_000282"
     CDS             complement(20010..20636)
                     /gene="gmk"
                     /locus_tag="VYN69_000282"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEV1532635.1"
                     /translation="MADRGLLIVFSGPSGVGKGTVRREIFESSENQFQYSVSMTTRAQ
                     RPGEVDGVDYFFRTREEFEELIRQGQMLEYAEYVGNYYGTPLTYVNETLDKGIDVFLE
                     IEVQGALQVKKKVPDAVFIFLTPPDLDELQDRLVGRGTDSAEVIAQRIEKAKEEIALM
                     REYDYAIVNDQVPLAAERVKCVIEAEHFCVDRVIGHYQEMLPKSPTTR"
     gene            complement(20766..22379)
                     /locus_tag="VYN69_000283"
     CDS             complement(20766..22379)
                     /locus_tag="VYN69_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEV1532636.1"
                     /translation="MEIMSLAIAVFAVIIGLVIGYVSISAKMKSSQEAAELMLLNAEQ
                     EATNLRGQAEREADLLVNEAKRESKSLKKEALLEAKEEARKYREEVDAEFKSERQELK
                     QIESRLTERATSLDRKDDNLTSKEQTLEQKEQSISDRAKNLDAREEQLEEVERQKEAE
                     LERIGALSQAEARDIILAQTEENLTREIASRIREAEQEVKERSDKMAKDILVQAMQRI
                     AGEYVAESTNSTVHLPDDTMKGRIIGREGRNIRTFESLTGVDVIIDDTPEVVTLSGFD
                     PIRREIARMTMEMLLKDGRIHPARIEELVEKNRQEIDNKIREYGEAAAYEIGAPNLHP
                     DLMKIMGRLQFRTSYGQNVLRHSIEVAKLAGIMASELGENAALARRAGFLHDIGKAID
                     HEVEGSHVEIGMELARKYKEPPVVVNTIASHHGDVEAESVIAVIVAAADALSAARPGA
                     RSESLESYIKRLHDLEEIANGFEGVQTSFALQAGREIRIMVNPGKIKDDKVTILAHKV
                     RKKIENNLDYPGNIKVTVIRELRAVDYAK"
     gene            complement(22570..22824)
                     /locus_tag="VYN69_000284"
     CDS             complement(22570..22824)
                     /locus_tag="VYN69_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEV1532637.1"
                     /translation="MLPKFTEDAWADYCYWQNQDKKTLKRINKLIKDIQRDPFTGIGK
                     PEPLKYDYQGAWSRRIDAENRLIYMMDGDSVAFLSFKDHY"
     gene            complement(22828..23082)
                     /locus_tag="VYN69_000285"
     CDS             complement(22828..23082)
                     /locus_tag="VYN69_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEV1532638.1"
                     /translation="MEAVLYSTFRNHLKDYMKKVNDEFKPLTVVNKNPDEDIVVLSKS
                     EWDSIQETLRIAQNKELSDKVLRGMAQVRAGSTQVHVIEE"
     gene            complement(23320..24417)
                     /locus_tag="VYN69_000286"
     CDS             complement(23320..24417)
                     /locus_tag="VYN69_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000156376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEV1532639.1"
                     /translation="MTITGIIAEFNPFHNGHKYLLDQAEGLKIVAMSGNFMQRGEPAI
                     VDKWTRAQMALENGADLVVELPFLVSVQAADFFGQGAVDILDRLGIDSLVFGTEEVRD
                     YQKIADLYTEKGTEMEKFVENLPDSLSYPQKTQAMWKEFAGLDFSGNTPNHVLALAYA
                     KAVAGRNIKLHPIQRQGAGYHSVNKDVDFASATALRQHQKDQDFLERFMPSVALFEQA
                     SKVSWEDYFPLLRYQILSNPDLTTIYQVNQEMAVRIKEAIKTAQSVEELVELVTTKRY
                     TKARVRRLLTYILVQARENVLPEAIHVLGFTEKGRQHLKSLKEQVNLVSRIGKEPWDA
                     MTQKADQIYQLGNPSIADQNFGRVPIRIETN"
     gene            complement(24427..25173)
                     /locus_tag="VYN69_000287"
     CDS             complement(24427..25173)
                     /locus_tag="VYN69_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000605145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1532640.1"
                     /translation="MIMATYETFAAVYDAVMDDSLYDKWTNFSLRHLPKTKERKKLLE
                     LACGTGIQSVRFSQAGFDVTGLDLSADMLKIAEKRATSAKQKITFIEGNMLNLSKAGK
                     YDFVTCYSDSICYMQDEVEVGDVFKDVYNALNEEGVFIFDVHSTYQTDEVFPGYSYHE
                     NAEDFAMLWDTYEDEAPHSIVHELTFFIKEADGSFSRHDEVHEERTYEILTYDILLEQ
                     AGFKSFKLYADFEDKEPTETSTRWFFVAQK"
     gene            complement(25276..25629)
                     /gene="rsfS"
                     /locus_tag="VYN69_000288"
     CDS             complement(25276..25629)
                     /gene="rsfS"
                     /locus_tag="VYN69_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEV1532641.1"
                     /translation="MNEKELLELVVKAADEKRAEDILALDVQDLTSVTDYFVITSSMN
                     SRQLDAIAANIREKVAQAGFKGSHVEGDAAGGWVLLDLGAVVVHIFSEEMRAHYNLEK
                     LWHEANSVDISEALA"
     gene            complement(25642..26142)
                     /locus_tag="VYN69_000289"
     CDS             complement(25642..26142)
                     /locus_tag="VYN69_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1532642.1"
                     /translation="MKTAFIIIDVQNVLVETGFQTKSLLGKISYLQNQARSKNIEIIY
                     VQHIENSEAQTSEDWQLSALLNRKPAEKVFQKKYNSIFKETGLKEYLDKQGIEKLVLC
                     GMQTEYCVDTSVKVAFEYGYQLIVPEGAVTTFVGDDIPAETINEFYEDIWEEHFADVL
                     DYKHIF"
     gene            complement(26143..26736)
                     /gene="yqeK"
                     /locus_tag="VYN69_000290"
     CDS             complement(26143..26736)
                     /gene="yqeK"
                     /locus_tag="VYN69_000290"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEV1532643.1"
                     /translation="MAYQDYINCSREALLEKMAELLPEKRLTHCLGVERAAMELAQRF
                     GVDVEKASLAGLLHDYAKKLSDQEFLDLIDRYQLDFDLKNWGNNVWHGMVGIYKIQED
                     LDLHDSEILRAIEVHTVGAGQMTDLDKVIYVADYIEHNRSFPGVDVAREIASLSLNKA
                     VAYETARTVEYLAHQGFPIYPQTLETYNAFVHYLKEN"
     gene            complement(26736..27365)
                     /locus_tag="VYN69_000291"
     CDS             complement(26736..27365)
                     /locus_tag="VYN69_000291"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEV1532644.1"
                     /translation="MTIELLTPFTKVELEPEIKEKKRKQVGILGGNFNPVHNAHLIVA
                     DQVRQQLGLDQVLLMPEYQPPHVDKKETIPEHHRLKMLELAIEGIDGLVIETIELERK
                     GISYTYDTMKILTEQHPDTDYYFIIGADMVDYLPKWYRIDELVDMVQFVGVQRPRYKA
                     GTSYPVIWVDVPLMDISSSMVRAFLAQGRKPNFLLPQPVLDYIEKEGLY"
     gene            complement(27417..27728)
                     /gene="yhbY"
                     /locus_tag="VYN69_000292"
     CDS             complement(27417..27728)
                     /gene="yhbY"
                     /locus_tag="VYN69_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEV1532645.1"
                     /translation="MSLTSKQRAFLNSQAHTLKPIIQIGKNGLNDQIKTSVRQALDAR
                     ELIKVTLLQNTDENIHEVAEILEEEIGVDTVQKIGRILILFKQSSKKENRKISKKVKE
                     I"
     gene            complement(27942..29048)
                     /gene="yqeH"
                     /locus_tag="VYN69_000293"
     CDS             complement(27942..29048)
                     /gene="yqeH"
                     /locus_tag="VYN69_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000391231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEV1532646.1"
                     /translation="MEEILCIGCGATIQTTDKAGLGFTPQSALEKGLETGEVYCQRCF
                     RLRHYNEITDVQLTNDDFLKLLHEVGDSDALVVNVIDIFDFNGSVIPGLPRFVSGNDV
                     LLVGNKKDILPKSVKSGKISQWLMKRAHEEGLRPVDVVLTSAQNKHAIKEVIDKIEHY
                     RKGRDVYVVGVTNVGKSTLINAIIQEITGDQNVITTSRFPGTTLDKIEIPLDDGSYIY
                     DTPGIIHRHQMAHYLTAKNLKYVSPKKEIKPKTYQLNPEQTLFLGGLGRFDFIAGEKQ
                     GFTAFFDNELKLHRSKLEGASAFYDKHLGTLLTPPNSKEKEDFPRLVQHVFTIKDKTD
                     LVISGLGWIRVTGIAKVAVWAPEGVAVVTRKAII"
     gene            complement(29051..29641)
                     /locus_tag="VYN69_000294"
     CDS             complement(29051..29641)
                     /locus_tag="VYN69_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEV1532647.1"
                     /translation="MDCFILVTKENCEKKLRKGARMAIENYIPDFAVEAVYDLTVPSL
                     QAQGIKAVLVDLDNTLIAWNNPDGTPEMKQWLHDLRDAGIGIIVVSNNTKKRVQRAVE
                     KFGIDYVYWALKPFTFGIDRAMKEFHYDKKEVVMVGDQLMTDIRAAHRAGIRSILVKP
                     LVQHDSIKTQINRTRERRVMRKITEKYGPITYKKGI"
     gene            complement(29593..30501)
                     /locus_tag="VYN69_000295"
     CDS             complement(29593..30501)
                     /locus_tag="VYN69_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEV1532648.1"
                     /translation="MVLEKQLGNGCTWIDLDLGKLNKLEDLSEIYGLDKETIEYALDR
                     NERAHMDYHRESGTVTFIYNVLDVKKDKAYYETFPMTFIVEHRRLITISNTKNAYVIE
                     QMTRYLENHDTLSIYKFLFASLEIISNAYYPVIEQMDKSRDEVNDLLRQRTTKKNLFV
                     LSDLETGMVYLTAAAKQNRILLEHIQGHALYRSFDEIEREQFDDAMIEAHQLVSMTDL
                     ISQILQQLSASYNNILNNNLNDNLTTLTIISVLLAVLAVVTGFFGMNVPLPLTDEPHA
                     WLYISLASAGLWIVLSLLLRKIAKKS"
     gene            complement(30558..31118)
                     /locus_tag="VYN69_000296"
     CDS             complement(30558..31118)
                     /locus_tag="VYN69_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEV1532649.1"
                     /translation="MQKFIQAYIEKLDVTTIIENILTKVISLLLLLIVFYIAKKMLHT
                     MVQRIVKPSLKMSRHDVGRQKTISRLLENVFNYTLYFFLLYCILSILGLPVSSLLAGA
                     GIAGVAIGMGAQGFLSDVINGFFILFERQLDVGDEVVLTNGPITVSGKVVSVGIRTTQ
                     LRSEEQVLHFVPNRNITVVSNFSRTD"
     gene            31257..32466
                     /gene="sstT"
                     /locus_tag="VYN69_000297"
                     /pseudo
     CDS             31257..32466
                     /gene="sstT"
                     /locus_tag="VYN69_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
     gene            32534..33487
                     /locus_tag="VYN69_000298"
     CDS             32534..33487
                     /locus_tag="VYN69_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1532650.1"
                     /translation="MSISQRTTKLILATCLACLLAYFLNLSSAVSAGIIALLSLSDTR
                     RSTLKLARNRLFSMLLALAIGVLAFHLSGFHIWSLGLYLAFYVPLAYKMGWEIGITPS
                     TVLVSHLLVQESTSPDLLVNEFLLFAIGTGFALLVNLYMPSREEEIQHYHTLVEEKLK
                     DILQRFKYYLSRGDGRNRAQLVAELDTLLKEALRLVYLDHSDHLFHQTDYHIHYFEMR
                     QRQSRILRNMAQQINTCHLAASESLILAQLFSKIAGQLSQTNPASDLLDEIERYLEVF
                     RNRSLPKTREEFETRATLLQLLREAKTFIQVKVDFYQKYRQ"
     gene            complement(33630..33707)
                     /locus_tag="VYN69_000299"
                     /pseudo
     CDS             complement(33630..33707)
                     /locus_tag="VYN69_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(33768..33992)
                     /locus_tag="VYN69_000300"
     CDS             complement(33768..33992)
                     /locus_tag="VYN69_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory protein Asp5"
                     /protein_id="HEV1532651.1"
                     /translation="MIEILIVLAIILSLALIVLVTIQPRQNQLFSMDATSNIGKPSYW
                     QSNTLVKVLTLLVSLALFVLLLIFMVITYK"
     gene            complement(33997..34176)
                     /gene="asp4"
                     /locus_tag="VYN69_000301"
     CDS             complement(33997..34176)
                     /gene="asp4"
                     /locus_tag="VYN69_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory secretory system protein Asp4"
                     /protein_id="HEV1532652.1"
                     /translation="MSEEDLFYKDVEGRMEELKQKPIKKEKETRGEKISKTFSLLLGL
                     MILIGLLFTLLGILR"
     gene            complement(34178..35515)
                     /gene="gtfB"
                     /locus_tag="VYN69_000302"
     CDS             complement(34178..35515)
                     /gene="gtfB"
                     /locus_tag="VYN69_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HEV1532653.1"
                     /translation="MIELYDSYSQESRDLHESLVATGLSQLGVVIDADGFLPDGLLSP
                     FTYYLGYEDGKPLYFNQVPVSDFWEILGDNQSACIEDVTQERAVIHYADGMQARLVKQ
                     VDWKDLEGRVRQVDHYNRFGACFAKTTYSADSEPIMTVYQDVNGQQVLLENHVTGDIL
                     LTLPGQSMRYFANKVEFITFFLQDLEIDTSQLIFNTLATPFLVSFHHPDKSGSDVLVW
                     QEPLYDAIPGNMQLILESDNVRTKKIIIPNKTTYERALELTDEKYHDQFVHLGYHYQF
                     KRDNFLRRDALILTNSDQIEQVEAIVEALPDVTFRIAAVTEMSSKLLDMLRYPNVVLY
                     QNASPQKIQELYQLSDIYLDINYSNELLQAVRQAFEHNLLILGFNQTVHNRLYIAPDH
                     LFESSEVAALVETIKLALSDVDQMRQALGKQGQHANYVDLVRYQETMQTVLGG"
     gene            complement(35508..37019)
                     /gene="gtfA"
                     /locus_tag="VYN69_000303"
     CDS             complement(35508..37019)
                     /gene="gtfA"
                     /locus_tag="VYN69_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HEV1532654.1"
                     /translation="MTIYNINLGIGWASSGVEYAQAYRAGVFRKLNLSSKFIFTDMIL
                     ADNIQHLTANIGFDDNQVIWLYNHFTDIKIAPTSVTVDDVLAYFGGEESHREKNGKVL
                     RVFFFDQDKFVTCYLVDENKDLVQHAEYVFKGNLIRKDYFSYTRYCSEYFAPKDNVAV
                     LYQRTFYNEDGTPVYDILMNQGKEEVYHFKDKIFYGKQAFVRAFMKSLNLNKSDLVIL
                     DRETGIGQVVFEEAQTAHLAVVVHAEHYSENATNEDYILWNNYYDYQFTNANKVDFFI
                     VSTDRQREVLQEQFAKYTPHQPKIVTIPVGSIDSLTESSQGRKPFSLITASRLAKEKH
                     IDWLVKAVIEAHKELPELTFDIYGSGGEDSLLREIIANHQAEDYIQLKGHAELSQIYS
                     QYEVYLTASTSEGFGLTLMEAIGSGLPLIGFDVPYGNQTFIEDGQNGYLIPSSSDHVE
                     DQIKQAYAAKICQLYQESRLEAMRAYSYQIAEGFLTKEILEKWKKTVEEVLHD"
     gene            complement(37031..39403)
                     /gene="secA2"
                     /locus_tag="VYN69_000304"
     CDS             complement(37031..39403)
                     /gene="secA2"
                     /locus_tag="VYN69_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HEV1532655.1"
                     /translation="MFRRLGQDFQLRKVKKILKQINALKGKMSSLSDQELVAKTVEFR
                     QRLSEGESLDDILVEAFAVVREADKRILGMFPYDVQVMGAIVMHYGNVAEMNTGEGKT
                     LTATMPVYLNAFSGEGVMVVTPNEYLSKRDAEEMGQVYRFLGLTIGVPFTEDPKKEMK
                     AEEKKLIYASDIIYTTNSNLGFDYLNDNLASNEEGKFLRPFNYVIIDEIDDILLDSAQ
                     TPLIIAGSPRVQSNYYAIIDTLVTTLVEGEDYIFKEEKEEVWLTTKGAKSAENFLGID
                     NLYKEEHASFARHLVYAIRAHKLFTKDKDYIIRGNEMLLVDKGTGRLMEMTKLQGGLH
                     QAIEAKEHVKLSPETRAMASITYQSLFKMFNKISGMTGTGKVAEKEFIETYNMSVVRI
                     PTNRPRQRIDYPDNLYITLPEKVYASLEYIKEYHAKGNPLLVFVGSVEMSQLYSSLLF
                     REGIAHNVLNANNAAREAQIISESGQMGAVTVATSMAGRGTDIKLGKGVAELGGLIVI
                     GTERMESQRIDLQIRGRSGRQGDPGMSKFFVSLEDDVIKKFGPSWVHKKYKDYQVQDM
                     TQPEVLKGRKYRKLVEKAQHASDSAGRSARRQTLEYAESMNIQRDMIYKERNRLIDGS
                     RDLEDVVVDIIERYTEEVAADHYASRELLFHFIVTNISFHVKEVPDYIDVTDKTAVRS
                     FMKQVIDKELSEKKELLNQHDLYEQFLRLSLLKAIDDNWVEQVDYLQQLSMAIGGQSA
                     SQKNPIVEYYQEAYAGFEAMKEQIRADMVRNLLMGLVEVTPKGEIVTHFP"
     gene            complement(39396..39836)
                     /gene="asp3"
                     /locus_tag="VYN69_000305"
     CDS             complement(39396..39836)
                     /gene="asp3"
                     /locus_tag="VYN69_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HEV1532656.1"
                     /translation="MIITQRQSIYWGEVGGTYMYGTTVSYYLDKSVRLYNPLLPSGET
                     LKTWFSSVNYQAARTQPQLPLLKRKQEYQLSLVFDCQPENGVYTKITFFDRYGDILEK
                     RVEKAKDFIFTYPEDSYTYQVSLLSAGFESLTFYHFSIKEIRSV"
     gene            complement(39833..41368)
                     /gene="asp2"
                     /locus_tag="VYN69_000306"
     CDS             complement(39833..41368)
                     /gene="asp2"
                     /locus_tag="VYN69_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HEV1532657.1"
                     /translation="MSKELNILQVGLTNWENHYDIPENMSWYYFYPNSSKALREIIEK
                     EDINRFHAVLIEDGQYAKDLFSYVKYFEPYTLFYNQNLQINDREVVDFLKKRCAQAID
                     FLSPQQLINDLSKSLFGGGYGDKLFPPTIQVNPNFTGAISYQGLDYVSLEGEFGQDFA
                     QLAYWAYNIMVQKTLPIELWLEYEKEGNCDFRLVIRKMWSGSVDEFFEEVIVSEKDLE
                     QALFMDSRDGDYFLSISVEARGRGTIKLGNLHQRWSRKQFGKFVLGGNILHDSKRDEI
                     NYFFHPGDFKPPLTVYFAGYRPAEGFEGYFMMKTLGCPFILFSDPRLEGGAFYLGTDE
                     LEGKVKDTITHYLDYLGFDHKDLILSGLSMGTFPALYYGASFEPHAIIVGKPLANLGT
                     IASRGRLDAPGVSNLAFDCLIHHTGGTSSQDMTELDQRFWKIFKQANFSKTTFGLSYM
                     KDEEMDPQAYEQLVSYLCNTGAKILSKGTAGRHNDDTDTNISWFLHFYRMVLETGFGR
                     EKR"
     gene            complement(41352..42932)
                     /gene="asp1"
                     /locus_tag="VYN69_000307"
     CDS             complement(41352..42932)
                     /gene="asp1"
                     /locus_tag="VYN69_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HEV1532658.1"
                     /translation="MYYFIPAWYGSERTWHADITPWYFSHFRLEFDDTFHQIRLFQEQ
                     DIDSRLLVLAYQPHLRYFLYRHGVLETDTYSVFDVMQDFHNLHTQVLSIRDIEWDDDC
                     EFIYSPFTIIVQKNGKKFAKVEHGVEGFISDIQYFGPNGQIHMHHIMDDRGFISSIIF
                     FEDGQAAYQEYLNPKGVWQFRERLKEGGQVEVNPILGYRFKMLTYQNMGDLVAEFFEN
                     YLQTYVKDQDIFMLPSHSHHDQLVLDRLPSTNPKLLSLFIGRNSQDTFRDLDVTFEKS
                     DLILVDREDSLRLLQELYPERMHQCYHLSSFDTRLRLGRSQTKKESIIYYQLDFEQGI
                     DNQALLQVLSFVAENKDTEVIFGAFAASQEQMNVVEGIVESFIQENIQSENLGKAIDY
                     GDAENPLEENQHQDLRIQFVNLNDELDLIKTLEFVRLIVDLNSHPHLYTQIAGISAGI
                     PQINLVETVYVEHLKNGYLLTDVTEFSKAAHYYTDRLKEWNEALIYSIDKIKEHTGQQ
                     FLGKLEKWIEEVKNVKGT"
     gene            complement(42942..44159)
                     /gene="secY2"
                     /locus_tag="VYN69_000308"
     CDS             complement(42942..44159)
                     /gene="secY2"
                     /locus_tag="VYN69_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HEV1532659.1"
                     /translation="MTKIYSSIAVKKGLFTLFLLFIYVLGSRITLPFVDLNTKDFLGG
                     STAYLAFSAALTGGNLRSLSIFSVGLSPWMSAMILWQMFSFSKRLGLTSTSIEIQDRR
                     KMYLALMIAVIQSLAVSLSLPVQSSYSVTLVALMNTLLLIAGTFFLVWLSDLNASMGI
                     GGSIAILLSSIVLNIPQDVIETFKLVYIPTGIVVLLVFMTIIFSYLLALMYRARYLVP
                     VNKIGLHNRFKRYSYLEIMLNPAGGMPYMYVMSFLSVPAYLFILLGFIFPNHSGLAAL
                     SKEFMLGKPLWVYVYISVLFLFSIIFAFVTMNGEEIADRMKKSGEYIYGIYPGADTSR
                     FINRLVLRFSVIGGLFNVIMAGGPMLFVLFDEKLLRLAMIPGLFMMFGGMIFTIRDEV
                     KALRLNETYRPLI"
     gene            complement(44216..45121)
                     /locus_tag="VYN69_000309"
     CDS             complement(44216..45121)
                     /locus_tag="VYN69_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001292324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1532660.1"
                     /translation="MSELISVVVPIYNTGKYLVECVEHILKQTYQNIEIILVDDGSTD
                     NSGEICDAFMMQDNRVRVLHQENKGGAAQAKNMGISVAKGEYITIVDSDDIVKENMIE
                     TLYQQVQEKDADVVIGNYYNYDESDGNFYFYVTGQDFCVEELAIQEIMNRQAGDWKFN
                     SSAFILPTFKLIKKELFNEVHFSNGRRFDDEATMHRFYLLASKIVFINDNLYLYRRRS
                     GSIMRTEFDLSWARDIVEVFSKKISDCILAGLDVSVLRIRFVNLLKDYKQTLEYHQLT
                     DTEEYKDICFRLKLFFDAEQRNGKS"
     gene            complement(45121..46317)
                     /locus_tag="VYN69_000310"
     CDS             complement(45121..46317)
                     /locus_tag="VYN69_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV1532661.1"
                     /translation="MRKSIVLAADNAYLIPLETTIKSVLYHNRDVDFYILNSDIAPEW
                     FKLLGRKMEVVNSTIRSVHIDKELFESYKTGPHINYASYFRFFATEVVESDRVLYLDS
                     DIIVTGELATLFEIDLKGYSIGAVDDVYAYEGRKSGFNTGMLLMDVAKWKEHSIVNSL
                     LELAAEQNQVVHLGDQSILNIYFEDNWLALDKTYNYMVGVDIYHLAQECERLDDNPPT
                     IVHYASHDKPWNTYSISRLRELWWVYRDLDWSEIAFQRSDLNYFERSNQSKKQVMLVT
                     WSADIKHLEYLVQRLPDWHFHLAAPCDCSEELTSLSQYTNVTVYQNVLHSRIDWLLDD
                     SIVYLDINTGGEVFNVVTRAQESGKKIFAFDITRKSMDDGLYDGIFSVERPDDLVDRM
                     KNIEIE"
     gene            complement(46326..47546)
                     /locus_tag="VYN69_000311"
     CDS             complement(46326..47546)
                     /locus_tag="VYN69_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 8"
                     /protein_id="HEV1532662.1"
                     /translation="MRNTKRAVVFAGDYAYIRQIETAMKSLCRHNSHLKIYLLNQDIP
                     QEWFSQIRIYLQEMGGDLIDCKLIGSQFQMNWSNKLPHINHMTFARYFIPDFVTEDKV
                     LYLDSDLIVTGDLTDLFELDLGENYLAAARSCFGAGVGFNAGVLLINNKKWGSETIRQ
                     KLIDLTEKEHENVEEGDQSILNMLFKDQYSSLEDQYNFQIGYDYGAAAFKHQFIFDIP
                     LEPLPLILHYISQDKPWNQFSVGRLREVWWEYSLMDWSVILNEWFSKSVKYPSKSQIF
                     KLQCVNLTNSWCVEKIDYLAEQLPEVHFHIVAYTNMANELLALTRFPNVTVYPNSLPM
                     LLEQIVIASDLYLDLNHDRKLEDAYEFVLKYKKPMIAFDNTCSENLSEISYEGIYPSS
                     IPKKMVAAIRSYMR"
     gene            complement(47577..50021)
                     /locus_tag="VYN69_000312"
     CDS             complement(47577..50021)
                     /locus_tag="VYN69_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_1767 family glycosyltransferase"
                     /protein_id="HEV1532663.1"
                     /translation="MIKTIVLAGDRNYIRQLETTIKSILYHNRDVKIYILNQDIMPDW
                     FRKPRKIARMLGSEIIDVKLPEQTVFQDWEKQDHISSITYARYFIADYIQEDTVLYLD
                     SDLIVNTSLEKLFSICLEEKSLAAVKDTDGITFNTGVLLINNKKWRQEKLKERLIEQS
                     IVTMKEVEEGRFEHFNGDQTIFNQVLQDDWLELGRAYNLQVGHDIVALYNNWQEHLAF
                     NDKPVVIHFTTYRKPWTTLTANRYRDLWWEFHDLEWSQILQHHMGEFELISPLDKEFS
                     CLTLTNSQDLEGIEELVTALPEVVFHIAAWTDMGDKLKKLAVYNNVRLHPQIVPPVLD
                     KLKKSTNLYLDINHGSADENFLKSLQEQEKTLLAFQSTQHGELGQIVFENGKVSFMID
                     TIKDFKKNGHLTCFRQLPSLTCLTFTASQDIEQLDYLAGQLPNVVFQIAAWTAMGPKL
                     YDLSNRYPNIQLYPAISRDKLDELKEKMDAYLDINLLTSTSDIVAEMAHLSKPILAFY
                     KSQNGNNGQRLYSSEHPERLLQAFHALIKGETLGEPEKPIKVIGIDQTLDYVIKNKSS
                     LVRFGDGEVNLMWGLPIPYQNHDLELANQLKHIVGLESDEKLVVCLPDAFDDRFVFTW
                     WATPFWKEHMNVYMDFYKELCKGSWYGSTFISRPYIDYEDKSKAKGQFEKLKSIWENR
                     DILIVEGITSRSGVGNDLFDKVKSVKRIICPSHNAYSVVDNIQEEIMKHAEGRLILCM
                     LGPTAKVLSYNLCQMGYQVLDVGHIDSEYEWMKMGAKTKVKFSHKHTAEHNFDQDIEF
                     IDDETYNSQIVARILN"
     gene            complement(50031..51041)
                     /locus_tag="VYN69_000313"
     CDS             complement(50031..51041)
                     /locus_tag="VYN69_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1532664.1"
                     /translation="MKLHLTNLYGMAGDSTVILAQNAVQKIASQLGFREVGIYFYNIA
                     SDSPSEMNKRLDGIMASISIGDILVFQSPTWNGFEFDRLLFDKLKDMQVKIICFIHDV
                     VPLMFDSNYYLMKDYLYMYNLSDVLIVPSERMKTRLMEEGLTTKKILVQGMWDHPHDL
                     SLYTPAFKKELFFAGSLERFPDLQNWSQDTPLRVFSNKGEASSSARNLSTEGWKKDEE
                     LLLELSKGGFGLVWGTHQNEGESNQYYTLNISHKVSTYLTAGIPVIVPSNLSTAKFIV
                     DQGLGFVANSLEEVHAIVDKMNLQEYQEMTNRIKTFSYLLKEGYFTKKLLVDAIYHLG
                     VE"
     gene            complement(51070..51945)
                     /locus_tag="VYN69_000314"
     CDS             complement(51070..51945)
                     /locus_tag="VYN69_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1532665.1"
                     /translation="MADELISIVVPIYNVENYLRMCLDNIQNQTYQNFECLLINDGSP
                     DHSSKICEEFVEKDSRFKYFEKANGGLSSARNLGIECSGGAYITFVDSDDWLEHDALD
                     RLYGALKKENADISIGRYNSYDETRYVYMTYVTDPDDSLEVIEGKAIMDREGVEEVRN
                     GNWTVAVLKLFKRELLQDLPFPIGKIAEDTYWTWKVLLRASRIVYLNRCVYWYRVGLS
                     DTLSNTWSEKRMYDEIGAREEKIAILASSDYDLTNHILIYKNRLQRVIAKLEEQNMQF
                     TEIYRRMMEKLSLLP"
     gene            complement(51950..53164)
                     /locus_tag="VYN69_000315"
     CDS             complement(51950..53164)
                     /locus_tag="VYN69_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEV1532666.1"
                     /translation="MYRKVIALGADIHYLDKVETVIKSVSVHNHEVKFYVFNDDLPSE
                     WFLLMRNRLKVIGSEIINVKKADHNLRDFHLPNAILSYATFFRYFIADEVLEDRVLYL
                     DSDIIVNANLDELFFLDLQGYAIAAVQDFDQDGWLTTFNSGMLLIDAKKWREKKSTQN
                     LLELTAQHHEHVYGDQGVLNIYFDDQWLHLDKEYNFMVGLDQFLHLKGNADWYQSNYY
                     GNYEPKIVHYTTEFKPWTHLTLTRFRKLWWFYYGLNWNDVLLSSDIVKRSFKELVGVP
                     LYHTCIFTNSAAMESLEYLLSELPEVHFTILAHTNFAPSVVDLQRYLNLSLFPNFNSF
                     NMKETLDKMDFYLDINHEGEIVNIIEEVCRRNKPIFGFDNTSHDSKGRSQIFSSTEPN
                     KMVEAIRQFIGE"
BASE COUNT        15575 a        11951 c         9622 g        16042 t
ORIGIN      
        1 tactattcta gtttcaatct gctatatcta ttatgcacat cactatagga tctaatgaaa
       61 atcacaacag actcattcat agatggttac ctcagggaac taagaaaacg actacaaagg
      121 aagtcgcatt catcgaaaag tggattaaca actatcctaa aaaatgcttg aactacaagt
      181 cccccagaga agacttctgg atgactaact tgaacttgaa atttagcaat aattaattca
      241 ccatctaact atatttagta attatttcag aactgattaa tattaaaatt aactaacaat
      301 tcaaaggatt catactagcc ataaattacg tccatcagag agagactctt actactttta
      361 gattttagtc tttctagctt cagaatacat ctaaacttta ggaaaaatga ctattcgaaa
      421 gcgcgaatgc ctcaaaatta tctcagataa gctattcgaa acttagaatg cttttaaatt
      481 tatggaattg cgattattcg aaacctagaa tgcatataac ctttagttga cagacctatt
      541 ctaagtctcg aagggctatt tactttctat tccttatcaa aaaagactca ttcccccttt
      601 ctcctccaaa atatggtata gtagaaatat actatctatg aggagtttac atgtcacagg
      661 ataaacaaat gaaagctgtt tctccccttc tgcagcgagt tatcaatatc tcatcgattg
      721 tcggtggggt tgggagtttg attttctgta tttgggctta tcaggctggg attttacaat
      781 ccaaggaaac cctctctgcc tttatccagc aggcaggcat ctggggtcca cctctcttta
      841 tctttttaca gattttacag actgtcgtcc ctatcattcc aggggccttg acctcggtgg
      901 ctggggtctt tatctacggg cacatcatcg ggactatcta caactatatc ggcatcgtga
      961 ttggctgtgc cattatcttt tatctagtgc gcctatacgg agctgccttt gtccagtctg
     1021 tcgtcagcaa gcgcacctac gacaagtaca tcggctggct agataagggt aatcgttttg
     1081 accgcttctt tatttttatg atgatttggc ccattagccc agctgacttt ctctgtatgc
     1141 tggctgccct gaccaagatg agtttcaagc gctacatgac catcatcatt ctgaccaaac
     1201 cctttaccct cgtggtttat acctacggtc tgacctatat tattgacttt ttctggcaaa
     1261 tgctttgaca cgtaaaaaat ccgtttggtt tcccaagtgg atttttaaag cgtagattaa
     1321 ctatagcttg atactaaata tactttggta tggaaatcat gcatattttt cgatagtgag
     1381 gcgaggactt acctagcctt tccgccgtga tagaaacacc tgaaatctaa tggtttcagg
     1441 tattcggaaa ctttgagaca ctaggctcaa agtttaggta tggaatttcg aagaaagtcg
     1501 ctaccgtccg taatcactta aggaaaggct caaaaatatt gttttcaacc acaaaatccg
     1561 tttggtttcc caagcggatt ttgtgcttta ttttgaaact tcttttgcaa gaacaaagtt
     1621 cccaagtgtg gcagaaccat ttcctgcgac tgctggcgtc acgatatagt cacgcacatc
     1681 tggtactggt aggtaaccat taagaagaga tgtaaatttc tcacggacac ggtccagcat
     1741 atgttgttga gccatgaccc ctccaccaaa gacaatcacg tctgggcgga aagtcactgt
     1801 cgcattaacc gcagcttgag cgatatagta ggcttgaaca tcccaaacag ggttgttgag
     1861 ttcaatagtt tccccacgta cacctgtacg agcttccaaa cttggaccag ctgcataacc
     1921 ttctagacat cccttatgga aaggacaaac acccttaaac tctttttcaa tatccattgg
     1981 gtgtctagca acataataat gacccatttc agggtgaccc acaccaccga taaactcacc
     2041 acgttggatg acgcctgcac cgatacctgt accgattgtg tagtaaacca agttttcgat
     2101 acgaccacca gcattgttac gggcaaccat ttcaccgtaa gcagagctgt ttacgtctgt
     2161 tgtgaagtac attggcacgt ttagggcgcg acgaagggca ccaagcaagt ccacatttgc
     2221 ccagtttggt tttggagtcg tcgtgataaa gccataagtt tttgagtttt tgtcaatatc
     2281 aatcggccca aatgaaccaa ctgcaagacc agcaaggtta tcgaattttg agaagaactc
     2341 aatggtttta tcgattgttt cgattggagt tgttgttgga aattgtgttt tttctacaac
     2401 gttaaagttt tcatcaccga cagcacagac aaactttgta ccgcccgctt ccaagcttcc
     2461 atataatttt gtcatgataa acctcttgtt tttattttct ttattatagc atacttcgaa
     2521 agtctaaatg tctctatttt ttagattttc ctctgtaaat cttactatct aataaaaacg
     2581 aacaaacatg tcatttgttc gttttcacat tagagaggat tgattagatt ttcacttcga
     2641 tcacagcatt ccccttcgca actgaacctg ttgcgactgg agctactgaa gcgtagtcag
     2701 ctgtatttgt aacgataacc attgttgtat catcaagtcc agctgcagcg attttgtttg
     2761 agtcaaatgt tccaagaaca tcgccagctt tcaccttatt accttgagca acttttgctt
     2821 caaaaccgtc accgttcata gatacagtgt caataccaac gtgaatcaaa acttcagcac
     2881 catttcttgt tttcaaacca aaagcgtgcc ctgttggaaa ggcaattgaa acttcagcat
     2941 cagctggtgc atagaccacg ccttggcttg gtttcacagc gataccttgt cccatagctc
     3001 cacttgagaa gactgggtca ttgacatcag caagagcgac aacatcacca acgataggag
     3061 ttacaagtgt ttcattttga agagctgctg gcgcaacttc ttctttttct tcagccactt
     3121 cagcttgttt tgcagctgca gttgcgtcta cttcatcttc gtaaccaaac atgtaagtaa
     3181 gagcaaaacc aagggcaaat gatacagcta ccataagaag gtattgtgga agttgtccgt
     3241 taccaacata aagcattgta ccagggatga tggtgatacc attaccagta ccagcaagtc
     3301 caaggataga agccaatcca ccaccgattg caccagcaat caatgaaagg aagaatggtt
     3361 tacggaagcg caagttcacc ccgaagatag caggctctgt aatacctagg aaggcagaaa
     3421 gagcagccgg gaaagcaagt gttttcagtt ttggattttt tgttttaaca ccaaccgcaa
     3481 cagtagcagc accttgagct gtcatagcag ctgtgatgat agcgttgaat gggttagcat
     3541 ggtcagcagc aagtaattgc acttcaagca agttgaagat gtggtgcaca cctgacacga
     3601 cgatcaattg gtgaacccca ccaatcaaga aaccaccaag accaaatggc atgctaagaa
     3661 tcgcttttgt agcaataagg atgtagtttt caacaacgtg gaaaactggt ccaatgacaa
     3721 agagtccaag gatagacatg accaaaagtg tcacgaatgg tgttaccaag aggtcaatga
     3781 catctggaac aaccttgcgg acagcttttt caaatttagc tccgacaacc ccgatgatga
     3841 aggctggaag aacggaacct tgcaaaccaa caacagggat gaaaccaaag aagttcatcg
     3901 ctgttacttc accaccttga gcaactgccc aagcgtttgg aagtgagcca gagacaagca
     3961 tcataccaag aacgatacca acggcaggat ttccaccaaa tacacggaag gttgaccaca
     4021 caaccaaacc tggcaagatg atgaaggctg tatctgtcaa gatttgtgtg taagttgcaa
     4081 agtcacctgg aagtggcatt tcaagagcgt tgaaaagacc acgcacaccc atgaagagac
     4141 ctgtcgctac gataactggg atgattggaa cgaaaacatc accgaaagta cggatagcac
     4201 gttggaacca gttcccttgt ttagcaactt ctgctttcat gtcatcctta gatgatgttg
     4261 gtaagccaag tacaacaact tcatcgtaca ttttgttaac tgtacctgta ccaaagataa
     4321 tttggtattg ccctgagtta aagaaagcac cttgaacttt ttccaagttc tcaatcactt
     4381 ctttattgat tttctcttca tctttgacca tgacacgtag acgagtcgca cagtgggcaa
     4441 cactattgac attttcacgt ccgcccaagg catcgatgac tttttttgca atttcctgat
     4501 tgttcatttg caaaaatctc cttatataac attttgttct tgtttgaaag cgattttatt
     4561 cgcccttacg actattattt tatcatgttt taaaaatatg tcaagcgttt tgcagaaaaa
     4621 atattctatc cacttagcgg gcaagcttta gattgattgt ttttgactgt ttcatcaggg
     4681 attccttgaa aaataagttt gaaaccttga ttaaaaagtc ttttatttca ttatctttca
     4741 cattttcgta aaaatttgat tgatttcctg tatttatttt atgataatcg tttgatagtt
     4801 tttcctcttt tttaacataa aaggcttgca ttttttatcg aaaacagtta ctattaaaag
     4861 tgataagatt tttggaggaa tgaatggaat ggacaactga gcgtcgttac agactttatc
     4921 aagattggac gcaagaagaa attcaacata taaaggaaaa tatggcacaa tctccatggc
     4981 atactcatta ccatgttgag ccaaaaacag gacttctcaa cgacccaaat ggcttttctt
     5041 actttgatgg caagtggatc ctcttttacc agaattttcc ttttggtgca gcccacggtt
     5101 tgaaatcttg ggcacagcta gaaagtgatg atttggttca ctttaaagaa actggaatca
     5161 aagttttgcc ggatactcca ttagatagcc acggtgccta ctctggttct gccatgcaat
     5221 ttggcgataa cttattccta ttttatacag gaaatgttcg cgatgaaaac tggatccgtc
     5281 acccatacca aatcggtgct ttgatggaca aggagggtaa gattacaaag attgacaaga
     5341 tcttgattga ccagccagca gactctactg accacttccg cgatccacaa atttttaact
     5401 ttaaaggtca atactatgct gtcgtcggtg gacaggactt ggagaaaaaa ggcttcgtcc
     5461 gtctctacaa ggctgtcaat aacgactaca caaactggca agcagttggc gaccttgact
     5521 ttgctaacga ccgtactgcc tacatgatgg aatgtcctaa tctggtcttt gtagaggaac
     5581 agcctgtcct tctctactgt ccacaaggat tggataagaa agttctagac tacgataata
     5641 tctttccaaa tatgtataag attggggctt cctttgaccc taaaaatgcc aaaatggtag
     5701 atgtgtctca acttcaaaac atggattacg gtttcgaagc ctatgcaact caagccttca
     5761 acgctcctga tgggcgtgct ctagcagtta gctggcttgg tttgccagat gtttcttacc
     5821 catctgaccg ttttgaccac caaggaacct tctctttggt caaggaactc actatcaaag
     5881 acgacaagct ctaccagtat ccagtcgctg ctattaagga ccttcgtgct tctgaagaag
     5941 ccttctcaaa ccgttcccaa accaagaaca cttacgaact tgaactcaac ttggaagcta
     6001 atagccagag cgagattgtc ttacttgctg ataaagaagg taagggactt tcaatcaact
     6061 ttgaccttgt aaacggtcaa gtgacagtgg atcgtagcca ggctggagaa cagtatgccc
     6121 aagaatttgg gacaactcgc tcttgcccta tcgagaatca ggctactact gctacaatct
     6181 tcatcgataa ctctgtcttt gaaattttca tcaataaagg agaaaaagta ttttctggtc
     6241 gtgtcttccc acatgcggac caaaatggta tcctgatcaa atctggaaac ccaactggaa
     6301 cttactatga attagattat ggtcgcaaaa ctaactgatg tcgccaaact tgcaggcgtc
     6361 agtcctacta ccgtttctcg ggttatcaat aaaaaagggt atctatctga gaaaaccatc
     6421 caaaaagtca atgaagccat gcgagaattg ggctataaac ccaacaacct ggctcgtagt
     6481 ctgcaaggaa aatcagctaa gttaatcggc ttgattttcc ccaatatttc caatgttttc
     6541 tatgcagaat tgattgataa attggaacac caactcttca aaaatggtta caagaccatc
     6601 atctgcaaca gtgaacatga ttctgagaag gaacgtgaat acatcgaaat gttggaagcc
     6661 aatcaggtgg acggcatcat ttctggtagt cacaacctag gaatcgaaga ctacaatcgt
     6721 gtgacagcgc cgattatttc ctttgaccga aacctatcgc cagacatccc tgtcgtctcc
     6781 tctgacaact atgctggtgg ggttcttgct gcccaaacct tggtcaagac aggtgcccag
     6841 tctatcatca tgattacagg gaatgacaat tccaattcgc caaccggact gcgccacgct
     6901 ggttttgcat ccgtactccc aaaagctcct attatcaatg tttccagtga cttttctccc
     6961 gtcagaaaag agatggaaat caagaatatc ttgacccggg aaaaaccaga tgccattttt
     7021 gcttcggatg atttgacagc tattctggtc attaaaatcg ctcaagaatt gggcatttct
     7081 gtcccaaaag agctcaaggt catcggctat gatgggacct actttatcga aaattactac
     7141 cctcaattgg ctactatcaa gcaacctttg gaagagattg cttgtctcac tattgatctt
     7201 ctcttgcaaa agattgaagg caaggaagtc gccacaactg gttacttctt accagttaca
     7261 ctattaccag gaaaaagtat ttaaacacaa gaaaactcag accgattcgt ctgagttttt
     7321 tatgatctta aattttcgag atagcgctgg gctgtctcta ggttaaaggt tttatctgcg
     7381 ataagctgct cgactagggg agcaacttca gattcactag ccccagctag gagagctagg
     7441 gatttggcct gtagtttcat gtggccttgc tggatgcccg tacttaccaa ggctttgagg
     7501 gctgcaaaat tttgggcaag accgatggac acgataatct gggctaattc tctggcagaa
     7561 ggatttccta gtagatcatg actgagagct acacgtgggt tgaggccgat agagccaccc
     7621 ttagtcgcta caggcatggg cagggtcatc tcaccgacca attcttctct ttcaaggttc
     7681 agcgtccagc aactaagacc ttgatagcgt ccatctcgac tggcaaaggc atgggcccca
     7741 gcttcgatgg cacgccagtc attaccagtg gcaatcaaaa tcgcatcaat accattaaaa
     7801 attcctttat tatgagtagc agctcggtaa ggatcagcct gcgcaaactg actagccaac
     7861 gcaattttct ccgcaatctc tcgtccttga tccttttggc ggctcaagta gcgaaaggcg
     7921 atgcgacagc ttgcagtcac cagagaatcg gtcgcgtagt tggacaggat tcccatgaga
     7981 ctctgtccct gactgagttc ttctaagact ggtttcaagg cttccagcat ggtgttgagc
     8041 atattggcac ccatggcttc ctgggtatcg acatgaatat aaacaacgag aaagtctggt
     8101 tcgcctttta tctgctcgac atgcagatca cgcgccccac ctccacgttt aacgatagaa
     8161 ggataggctt gattggcaag ctccaagagc tccgctttct tgctggcaat cttctcttgc
     8221 gctagtttag gattagcaac ttgataaagg gctacctgcc caatcatctg tcgctgatgg
     8281 acttgtgcag taaaaccacc tgcacgcttg atgattttgc tggcatagct ggccgccgca
     8341 accacagagg gttcttctgt cacataggga acggtgtatt cctgaccgtt gacaagtacc
     8401 tccggaacca gtgaataagg cagagaaaaa gttcccacta cattctcact cagctggtct
     8461 gccacagtca cgctcatctg ttcatccttc tccagactag cttgtctctc aggactaagg
     8521 agcgcctgag cttttaacag ctcgaggcgc tcttggtatg attttttaga aaatccattc
     8581 caacttatct tcattatttt tcaaccttgc tataacggcg ttggtggtcg agaatttcaa
     8641 ccaaggcaaa atcttgattt tcatagccag caaactgggc agagtttgtt tcatccaagt
     8701 ttacttcctc aaaaaagacc ttttcatagt ctgcaacgga taagacagtt cgttggttga
     8761 gcttgttcaa gcggtcttta tccaaataag cttcatatcc ttcaaccaat tcaccactga
     8821 agaactcagc cacagctcca cttccgtaac tataaagggc gattttatcc ccagctttca
     8881 agctatctgt attttccaag agagacaaaa gtccaaggaa aagtgaacct gtgtagatat
     8941 tccccacctt ttgactgtag agaatagact ggtcaaaatg cttttgtaag aggtcttttt
     9001 tctcttgagg caggctctta tccatgattt ttttcaagcc ttttagcgct aatttaggat
     9061 aaggcaagtg gaaacaaaca gccgcaaaat catccaaagt aagctggtag cgtttttgat
     9121 attcaagcca agtcgttttc aaactatcca agtattgttg ggtagaatag acaccattta
     9181 cataaggagt tgtcgagtaa tttggtcgcc agaaatccat gatgtcacgg gtctgagcta
     9241 cattgtcatt attaaaggcc atcatgcgtg gattttgtgt aatcaacata gccacacttc
     9301 cggcaccctg agtgggttct cccggagttt caataccgta tttggcaata tcacttgcaa
     9361 tgaccaagac cttggactcc ggagaatttt ccacatgcaa tttggcataa tggagggcag
     9421 cagtcgcccc atagcaggct tctttaatct cgaaactacg agcaaagggc tggatgccca
     9481 gcaagccatg cacaaagacg gccgcagcct tactctggtc aattcctgac tcggtcgcca
     9541 caatgaccat gtcaacttct tgtctttctt gctcagttaa aatagagtca ctagcactgg
     9601 ccgccaaggt cacgatgtcc tcagttaggg gcgcaatact caattccttg agtaagagtc
     9661 ctttacttaa tttttcaggg tcaattcccc tcgcttctgc taagtcttgt aatttcaaga
     9721 catattgact ggtcgcaaaa ccaatcttat caataccgat tgtcatattt acctctgttt
     9781 tatcattcat gtaaaaaatc gttctatact attttatcac aaatggcagt aaaagagaga
     9841 aaaaagactt gattcaccaa atcaagcctc ttattggtca tcattttaaa gaatgattag
     9901 ttgctagaga gttcaccgat ataagtagct ttataagctc cattcacagt tatcagctcc
     9961 tggaggatca aatttcctga gtaagtcctt cccatctcat ctacaaattt ttgataaaac
    10021 tgactggtcg gaatttctct gacatcctta tcaaatgtct tgtaaagtgt tttactaacc
    10081 ttctcagcaa tcaattgatg ctcttgccat ccactttgaa actctgagcc cgaactagaa
    10141 accatgactg ggataaacaa caaggtcagt agacttacag acaataagga aaatagtaga
    10201 cttcctgcaa aactagaatc ctagttcatg attgataata ccagcaatca aattcattcg
    10261 taatccgaag cgtttacgat gatttcgata ggttgttgaa aacattttaa acgtttttac
    10321 tttggcaaag atattctcaa ccttgcttct ttccttagat agtgcatggt tataggcttt
    10381 atcttcaact gttagcggct tgagtttgct ggatttacgt ggagtttgtg cttgaggata
    10441 tatcttcatg ggcccttgat aaccactgtc agccaagatt ttaccaactt gtccgatatt
    10501 tctgcgactc attttgaaca acttcatatc atggcaatag ttcacagcga tatccaaaga
    10561 aacaattctc ccttgacttg tgacaatcgc ttgagccttc atagcgtgaa atttcttttt
    10621 accagaataa ttggctagtt gattttttta ggacgattta tttttacctc tgttgcatcc
    10681 acaatcaccg tattctcagc actaagatga gtttttgaaa tcgtaacacc actttgaaca
    10741 agagttactt caacccattg gctccgacgg attaagttgc tttcgtggat accaaaatca
    10801 gccgtaattt cttcataagt gcggtattct cgcacatata gaaagcgttg ttaatttatt
    10861 tatctcattt ttcagaaaat tcttttattt ctgtaaagtc tacgatactc gatgtgtttt
    10921 tatataatga tacagtctga gaatcactgt tccgctagcc attccaatag agattaccag
    10981 agccaacatg acaaccaagg tcgcacttgc cagtgcttta ttatagtccc ctgtcacaaa
    11041 aaaggcagtt gttcggtagg agagataacc tggaaccagc ggtgccaaaa tggccaagat
    11101 aaagaccaca gcaggtgtct tataaagaat acttaaaatc tggctgacac aagaaccaat
    11161 aatggctgca atgaaggtag ctacaatgac attggtcggt tccttgagca agagatagat
    11221 tagccagaca gtcatgccca aaatccctcc aggtaagagc atagaccgtt gcacattgag
    11281 tacgattaaa aaagtgataa tggcaagaaa acttgctact gcttgtaata aaaaggttgt
    11341 tagtgtcata ttagttcatc aataccaagg cgacagaagt tcctgcccct aaagcgaggg
    11401 taatgagcag ggattcaaac atcttactca taccagagtt tatgtggttg gtcataatat
    11461 cacggaccgc attggtcaag gcaatgcctg gtacaaacgg catgaccgca ccagctataa
    11521 tcaaatctgc cgttgaagga aaacctgtgt agcgagccca aaactgggca attatcccaa
    11581 agacaaaggc tccagcaaag gctgtcacaa agggaattcg gataaatttt tccacataga
    11641 gggaaaaggc aaaaccaaat aaggtcgcca ctcctgcccc aagtgcgtcg tagatatttc
    11701 cgctaaacat aactgaaaag aaaggagcac taaaggtcgc agccagagtt acctgcaact
    11761 tagtataggg aaggggttga gcttgcaagg ccgtcaattg cttaaaggct gtttctaagt
    11821 caatctgccc cccaactagc tgacgagaaa tctggttcac atcgcagact ttttcgatgt
    11881 tataagaaga ggaggtcacg cgcttcatgc gcgaaatatt ggtattttca atagagaaaa
    11941 agatagcggc aggcatggca aggacattgc aatccacaat cccctgcgaa tgcgcgattc
    12001 gaatcatggt atcttctaca cgatggattt ctgagccact tttaaggaga atagtccccg
    12061 ctagcataat cacatcaatg acggcattta attcttttga ttcttccatg ctttcctcct
    12121 tttatcaact ccctctattc tatcacaaat ctggactcaa aaaaaatctt tgccatgaaa
    12181 tcatgacaaa gattgattac tcattttgat tatccatctg cttttaagga gtagctgaag
    12241 ttgttttagg tttgtagatt gaaatcttga ctctagtctt atttaggtct accttttcac
    12301 ctgctctagg actttgttca acaaccatgc cttctacact acctgcaggc gctgtcgtca
    12361 cttctacaac ttctatatta gcttccttaa tcccaacaat ttgaatcaaa ttgttcttag
    12421 taaactccaa gctggaacca atgtaactcg gcatggcaac acttgtaact tttttagcta
    12481 ctgtcaagac aatttgagta ggtttactca catcataagt cgttccggca cctggacttt
    12541 gtttcataat cgttcctggt tcgctttcgc tggactcttc ttcctctatc ttaatcaaat
    12601 tctcaggaac cttcttctgc ttgagttctg agattacttc tgtagagttc cgtccaatat
    12661 agttccctaa ttgaatcgtc gtagcttttt tagctactgt caaaacaatt tgagttgcct
    12721 tgctcaagtc ataggtcgta ccttctggta gactttgctt caggaccgtt ccagcctcac
    12781 tttcattcga ctcttcttcc tcaattttaa tcaaattatc tggaactttt ttctctttta
    12841 attccgcgat aacatcagaa gatttccggc cgacataatt actaatttgg aaggattgtt
    12901 tgcctgatga gacaaccaga ttaattttcg ttccttcttt tcgaccagtt ccagcgccag
    12961 gatctgtacg gataatccgc ccttcttcca ccttttcact agcctctgtc ttctcctcac
    13021 caatctcaaa attggctttt ttgagcgttg ccttggcctc tgcaactgtc tgacctgcca
    13081 catctggaat ggcaatggtt gcaggagttc tggatagtat ccaaataaga gaagctgcca
    13141 ccaatacaag gctggccaac aaaatcaggt aacgcatctt aaatctatgt tttttcggtg
    13201 cttgtggttg gtaagtttcc tctgtcacag cctggcttgg gtttttgatt gatttgtgtt
    13261 ctgtctgcgc ttgaacctta ggaatagatg tcaaggtact ctgagaaacc ttcggcaagg
    13321 tcttggtatc tgccttgctc gtttcatcaa agattaactt actttcattt ctacgattgt
    13381 aggacaagct actagacaag tccacataca tctctgaaac cgagcggtag cgattggtca
    13441 actttttagc agtcgccttg ataataacat tttctaaagc ctgaggtaca gatggatttt
    13501 ctgcaataac ggacggcagg ggtttctgga aatgctggag ggcaatggtc accgcgctat
    13561 ccccgtcata agggatatgg cctgtcaaca tctcatagaa aataatcccc atggcataga
    13621 tatcactctg cacagtcgcc ttcgaaccac gcgcctgctc tggtgacaag taatgaactg
    13681 agcccaacat cgagttagtc tgggtcagac ttgtctctgc aaaggctaca gcaatcccaa
    13741 agtctgtgac cttggcagtc ccatctggtg tcaaaaggat attttgaggt ttcaagtccc
    13801 tgtgaacaat tcctcgagta tgggccaagc gcatagccaa gagaatttgt cccatgatac
    13861 ggactgcttc ttcattagaa agaggataat gttccttgat atagcgtttg aggtctagtc
    13921 cagcaacata ctccattgca agatactgtt gaccgtcttc ctcaccaata tctgttatcc
    13981 gaacgatatg aggatggtct agatctgcca tagctctcgc ttcacgctga aaacgagcta
    14041 cagctatcgg gtccgtctgg tagttggtcc tcagaacctt cactgccact tcttccccat
    14101 ctaagattaa gtctttggct aggtagacat ccgccatacc tcctcgacca atctgtttga
    14161 caatccgata gcgtccggca aaaatcttgc cgatttggat cattctgcat cctcctcgtt
    14221 tatagaaaca agggcaaccg taatgttgtc taaacctcct gcattgttag caaaacgaac
    14281 aagtgtctcc gttttatctg ctaaaggaat atcactggtt acaatatcac gaatctcact
    14341 gcctgaaatc atgttggtca agccgtcact attgagcaag agatagtcac ctgactcaag
    14401 gataactgtc ccaaaatcag gctgaatttc atctttttgc ccaatagact gggtgataat
    14461 atttttttgc ggatgagctt ctgcctcttc tggtgtcaat tgaccagcct tgagcaattc
    14521 attaaccaaa gaatgatcac tcgtcaactg atggtattct tctccacgaa tcaagccgat
    14581 acgcgaatca ccaatatgag catagatagc ctgattatca ataatagcaa ggacttccaa
    14641 agtagttccc atgcctctgt aagcttcatc ctgaccaagc tggtgaatct tttgattttc
    14701 aatttctagg taatgggcga accattcacg cacttcattg actgtatcga tctgagtatc
    14761 aacccaagct acacccaggt ctgtgaccgc catttcacta gcgatattcc ctgcgcgatg
    14821 acctcccatc ccatcagcta aaataatcat ggtacgtcca gctctattga catagtggtt
    14881 gacatagtct tggttatttg ttcgtttctg accaacatct gttaataatg aaatttccat
    14941 gtgtcagttc cttcctaatc cgatatcttg cgaaattgac tgatgaagaa tccatcactt
    15001 ccatacaatt caggtgtaat gaggatacag ccgtctttca tgatatcctt acattcatgt
    15061 tctagtttta cctgctcgaa ctcgggatga ctctctaaaa aggccttaac gacttgaaaa
    15121 ttctcctctg agacgatagt gcaggtgcta taagttatta taccaccttt gcctagtatt
    15181 tgacaaacac tacctaatat ttctaactga atttcctgca aggacgcgaa atctgccgtt
    15241 tctttattgt atttgatatc tggttttcgg cgcaaaagac cgattcctga acaaggagca
    15301 tccaccaaaa tcttatcaaa ggaatcctgg tcaaaaaact catgcacctt tctggcatcc
    15361 aatttttgag tttgaacccg atcttcaact cccagacgtt gggcattttc ttgaattaaa
    15421 tccaacttgt ggtcgtacaa gtccagagca gtaacctgac ctgtcgtaag ataagaggct
    15481 atatgggctg ttttcccacc tggagctgca caggcatcaa gcactcgctc atcaccttgt
    15541 aaatcaagcg tcggagcaac cagctgactg gactcgtctt ggatggtaat ggctccatcc
    15601 gcaaacaaat tatgccctgc aaaatgcccc tgctccttaa ccagaccagt ggttgctaaa
    15661 agggaatcat tcgcctccaa caaggcttgg atttcctctt ttcgacttag gtctgttaca
    15721 cgaatactgg ctttgtttcg cactaacagg ctttcaaaga tggcttttgc tctctcctct
    15781 ccgtattctt ccttgagttt ggcaactagc caaactggga gagaataggc aatggagtca
    15841 cgcttgtttt ttcgcttgat gctagcaata tctggccagc cttcacgcaa gatacggcga
    15901 aggacagcgt tgaccaattt ttcactgcct tttttacgga gtttggctaa ttccactgct
    15961 tcattgacca cagcatgatc tggaattttg tccaaatagc ggagttggta ggcactcatg
    16021 agaagaagga catagagcca gctgtctaac tggtctctgt cttcgataaa gtgggatagg
    16081 taccattcca gagtcagttt acgggctacc gttccataga ctagctcggt cactaagccc
    16141 ttgtctgctg cagagagctg actccccttg aggtgtttat ttaaggcaat atttgaatat
    16201 gcttgattca caaaaacatc ctctagcact gctagagcta aacttctagc cgtttctact
    16261 ttagtcacca aatcgttctc ctacagtcaa tgtacgtcca actccattga ggaaggaagc
    16321 aatgtccatc ttaggcttac cagctggctg cacttgtttg agggatagag ccccttcagc
    16381 cgttgcgaca atcaattctt tcttgccgat agagagaatc tcacctggat ttccctgacc
    16441 ttctactggt agggcttcat aaatcttaaa gcggtcgccc ttaaggaaag tatgggcaac
    16501 aggccagggg ttcattccac gaatttggtt aaagagttga cgattggttt tgttccagtc
    16561 cagtttttct tcctttggct ttatatttgg agagaaggta acctgactcg tatcctgcgg
    16621 ttcaggtttg atatcaccag caatataggc aggcagagtg tccaaaagca aatcacaacc
    16681 aactagcgcc aatttttcaa acaaggtgcc aacattgtcc tcatctgtga tcggaatgct
    16741 gcgacgagaa atcatatctc ctgcatccat ttccttaacc atttccatga tggtcacacc
    16801 agcttcctca tccccttgaa tcaaggcata atggataggc gcaccaccac ggtgtctagg
    16861 aaggagggag gcatgaacgt tgacagcaaa gtccatgcta tcaaggagtt tgcttgggag
    16921 aaactgccca aaagcagcag tcacaattcc atctgctcct agcttcataa gatcttccat
    16981 ctctggactt ccagataatt tttcaggttg gtagatagat agtcctgctt ccttggcagc
    17041 ctgcttgact ggggtttctt ggataacttt tttacgacca acagcacggt ctggctgggt
    17101 cacaacggct agaatttcgt aacggtcatc tgtcaaaagt ccttttaaga ctgttgctga
    17161 aaagtcgggg gtccccataa agattagttt tgtcatatct tctccttctt ataaaaattg
    17221 ctgcggctca tggtcaatgc tgagacggag ctcactattt tcccgttctt gagtcaaggc
    17281 caagacctgg ttgagggtcg gccccagctc atcttctaaa cggtatttaa ttaaaatctg
    17341 gtaatgatag aggttgtggg tacgggcaat cggttttggc gttggcccca gaatgggact
    17401 ggtctctgac aagcctgacc gcaaaatgtt catgacttca taggcacgtt tgaaaacctc
    17461 ttcttctttc ttgtgagaaa gggtaatacc aatcgtgaaa tagtaaggtg gatagccgag
    17521 ttgtcgtctg attcccattt cataggcata aaagccttcg taatcttgat ccttggcaaa
    17581 tcgaatagca tagtgctgcg gattgtagga ctgtatcaag acttgacctg ctttttcagc
    17641 ccgacctgct cgacctgcca cctgagtcaa gagctggaag gttctctcag aagaacggaa
    17701 atcaggcaga ttcaaggccg tatccgcatt tagaactccg actagggtaa cattgggaaa
    17761 atccaaaccc tttgcaatca tctgagtacc aagtaaaata tccgcttccc ctcgcccaaa
    17821 ctggtcaagc aaggcttggt gactgccttt ctttcgagtc gtatccacat ccatcctcaa
    17881 aatgcgagct tggggaaaga gttctgctag ctcatcataa gccttctgag ttcccgtccc
    17941 atagtaacga atactgcggc tcttacagtt aggacagacc tgaggaatat ccttcgagaa
    18001 accacaataa tggcagttca tagtcttggt atccatatgc aaggtcagag aaatatcgca
    18061 gttgggacaa gtatccaccg tcccacactc ccgacacatg acaaagctag aataaccacg
    18121 gcgattgagc atgagaacca cctgctcttt tttaaccaga cggtcttgga tagcctctag
    18181 caaaggaggc gtaaagtttg acgtctcatt ttgtccgata tagtctcgaa agtcaatcac
    18241 ttgaacctca gggattgtag ccaaaggatt ggcacgttgg gttagacgta agtgttgata
    18301 gacgcctttg ccagcacgtg cccggctctc taagctcggc gttgcagatc caagtaccag
    18361 agttgcttga ttatactgag cccgtaaaat agctacctct ctggcatggt aacggggatt
    18421 gctgtcctgc ttataagccg cttcatgctc ttcatcaata atcatgacac ccagattttt
    18481 cagaggagca aagatagcag atctggcacc aacaacaact tgggcatcgc cacgctccac
    18541 cttgcgccat tcatcatact tttcgccatt ggataatcct gagtgaagaa tggctacctt
    18601 gtccccaaaa cgtgctataa aacgctcggt catctgagga gtcaaggaaa tctcaggtac
    18661 cagcaaaata gctgtcttac ccttatccag ggcaccttgg ataatctgca agtaaacctc
    18721 ggtcttccca cttcctgtaa tcccttgaag tagaaaggga ggttgagaac tgccaataga
    18781 actcacaacc gcatcacgcg cctgtctttg ttctggattt aactccaaag gtctacttgc
    18841 ttcaattcct tcaaaataag cagccgagcg ttgaacttcc ttttggacta tggtaacagc
    18901 accttgatcc acaaagaagt tgacttgctc tcgcgagtag gactctaaca agctagccaa
    18961 ggaagcgctc tctggatgag acagcagata atctctcagt tccaactttt tcttggcacg
    19021 tgtagaaatc tcaacacctt ctaattgggc atggtcaacc tcataccaag actgggtctt
    19081 gaccttcttt tgatcgactg cctgatattc cagaccaagc aggccttttc tagtcaaacg
    19141 catcatttca gcttgcttgg caaggtctag tgaagaaaag gctagcgaat cttctgaacc
    19201 aaacaggcgt actcgttctt cctgactcaa gccttccaga ggatagagaa tcttgtcata
    19261 gctagaattc agaaaccctg gaagcatggc cttgaggata gagattttgt aggagaagac
    19321 agatttgcgt aactcctcag ccagccagag ttgttctggc gtgagaacag gagaaaaatc
    19381 cagcacctct gcaatatctt ttaaatcttg ctccatctct tctccatctg attgggactt
    19441 caaaccaaga acaatccctt gaatcaggcg attaccctta ccaaaaggca catgaacccg
    19501 catcccaact tccagcattc cctcaaattc ctccggaatc ctgtaactat agggctggtc
    19561 cgtctgcatc aagggcacat ctacgataat cttagctagg gccatcttct cacctcctcc
    19621 ttgtcagtac attcttgcaa tagaaaaaat aagattgagt ccccccaacc ttaaattttt
    19681 tcaccatctt ctttttcttt agcaatttgc tctttgattt tcttttcttc ttcttctttg
    19741 cggcgttttt cttcttcgat acggcgacgc actgcttcac gttttccttc tggatctggg
    19801 tgaattgtaa cgtttcctga ttcgatttct tctaaagcgc gaagagttga tttttcagac
    19861 ttgaaacctt gagttgctgg ggcacctgct tccaattcgt gggcacgttt tgcttccaag
    19921 attacgagtg aatattttga aggaaccttg tcgagcaagg tatcaataga gggttttaac
    19981 atcatttgct tgtacctatt ttctaaattt tatcgggtag ttggagattt tggtaacatc
    20041 tcctgatagt gaccaatgac acgatccaca cagaagtgtt ctgcttcaat cacacatttg
    20101 acacgttcag cagctagggg cacctgatcg ttgacaatcg cataatcata ctcacgcatg
    20161 agggcaattt cttccttggc cttttcgatt cgttgggcaa tcacttctgc actatctgtt
    20221 ccacgaccta ccaagcgatc ttgcaattca tccaaatctg gtggtgtcag gaagataaag
    20281 acagcatctg gaaccttttt cttgacctga agagcaccct gaacttcaat ttcaaggaaa
    20341 acatcgattc ccttgtccaa ggtttcattg acataggtca gaggagttcc atagtagttg
    20401 ccgacatatt ctgcgtattc caacatctgt ccttgacgaa tcagctcttc aaattcttca
    20461 cgagtacgga agaaatagtc aacaccgtcc acttctccag gacgttgtgc gcgtgtcgtc
    20521 atcgatacag aatattgaaa ttggttttca gaactctcaa aaatctctct tctaaccgtt
    20581 ccttttccaa cccctgaagg accagaaaaa acgattagta agcctcggtc tgccattgtg
    20641 tctcctttta gtcaatctgt gaaataacat ttctctagaa taatggcaaa aagccagatt
    20701 atcctttaca gtctttctat ctagtgtaac aaaaaagcag taatttttca actgctcttt
    20761 cttatttatt tagcataatc tactgcacga agctcgcgaa tcacggttac cttgatattt
    20821 cctggataat cgagattgtt ttcaattttc ttacgaactt tgtgagccaa gattgtgact
    20881 ttgtcgtcct tgatttttcc tggattgacc atgatacgaa tttcacgtcc tgcttgaagg
    20941 gcaaagctag tttgcactcc ttcaaagccg ttagcaattt cttccaaatc atggagacgc
    21001 ttgatgtagc tttcaagaga ctcactacga gcacctggac gggctgcgct caaggcatct
    21061 gctgcagcga cgataactgc tatcacgctc tcagcttcaa catctccgtg gtgactagca
    21121 atcgtattca ccacaactgg gggttccttg tacttacggg ccaattccat accgatttca
    21181 acgtggctac cttcaacctc atggtcaatg gctttcccga tatcgtgaag gaatccagca
    21241 cgacgggcaa gagccgcatt ttcaccaagt tcgctcgcca tgataccagc caacttagca
    21301 acctcaatcg aatggcgcaa aacattttgt ccatatgaag tacggaactg caaacgtccc
    21361 ataatcttca tcaagtctgg atgaaggttt ggcgcaccaa tttcataggc agcagcctca
    21421 ccgtattcac gaatcttatt gtcaatctct tgacggtttt tctcaaccaa ctcttcgata
    21481 cgagctggat gtatacgacc atctttgagc aacatttcca tagtcatacg ggcaatctca
    21541 cgacgaatcg gatcaaatcc tgacaaggtc accacttctg gtgtatcgtc gataatcaca
    21601 tcgacccctg tcaaactttc aaaggtacga atgttacgac cttcacgacc aataatgcgt
    21661 cccttcatag tatcgtctgg cagatgaacc gttgagtttg ttgactccgc tacatattca
    21721 ccagcgatac gttgcatagc ttgaaccaag atgtccttgg ccattttgtc agaacgttcc
    21781 ttgacctctt gctcagcttc gcgaatgcga ctggcaatct ccctggtcaa gttttcctct
    21841 gtctgagcca agataatatc tcgtgcttct gcctgagaca gagcaccaat acgctctagt
    21901 tctgcttctt tttgtctttc gacttcctct aattgctctt cacgcgcatc aaggtttttc
    21961 gctctatcag aaatactttg ttctttttgt tcaagtgttt gttctttact cgtcaaattg
    22021 tcgtccttac ggtcaaggct agtagctctc tctgtcaaac gactttcgat ttgtttgagt
    22081 tcttgacgtt ctgatttgaa ttcagcgtcc acttcttcac ggtattttct ggcttcttct
    22141 ttggcctcca atagtgcttc ttttttaaga gacttgcttt cacgtttggc ttcattaaca
    22201 agtaaatccg cttcacgctc agcttgtcca cgtaaattag ttgcttcttg ttcagcattt
    22261 aaaagcatca actctgcagc ttcctgagat gatttcatct tagctgagat gctgacatat
    22321 ccaatgacta aaccaatgat gacggcaaaa acagcaatcg caagcgacat gatttccatg
    22381 tttttacctc attttattgt tattccgaat gacatacatt cttttacatt ctaccataaa
    22441 aaagtgattt tcacaaacct aaaatagaat atgttttggg gaatttggaa cacatttacc
    22501 aaaataaact tgttgtttag aaatagtagt ttagtagaga cttgagaaaa agcctacctt
    22561 tcaatagact tagtaatgat ctttaaagga caagaaagcc acactatctc catccatcat
    22621 ataaatcaag cgattttctg catcaatacg ccgtgaccag gctccttggt aatcatattt
    22681 gagtggttct ggtttaccta ttcctgtaaa gggatcacgt tgaatatcct tgattagttt
    22741 attgattctt tttaacgttt tcttatcctg attttgccag tagcaataat ctgcccaggc
    22801 atcttctgta aacttgggca gcatttctta ctcctcaata acatggacct gagtacttcc
    22861 agcacgaact tgagccattc ctcgcaaaac cttatcagaa agttccttat tttgagcaat
    22921 tctcagggtt tcttggatac tatcccactc actctttgaa aggactacaa tgtcctcatc
    22981 tggattttta ttgaccaccg tcaaaggctt aaattcatca tttaccttct tcatgtagtc
    23041 ctttaaatga tttcggaatg ttgagtaaag gactgcttcc ataaccatac ctcgttttag
    23101 ctcttttcca ctattataca cgaaaagaaa gaaattgtca ggaacttgta caagattttc
    23161 ttttctatct atttatactc aatgaaaatc aaagagcaaa ctaggaaact agccgcaggc
    23221 tgtacttgag tacggcaagg cgacgttgac gcgatttgaa tttgattttc gaagagtatt
    23281 attcgtaaaa aatctcaaaa agcctacctt tcggtagact tagtttgttt ctattctaat
    23341 cggcactctt ccaaaattct ggtctgctat acttggattt cctagttggt aaatctggtc
    23401 tgctttttgg gtcatagcat cccaaggttc tttgccaatt cggctgacta gattgacctg
    23461 ttctttcaga gacttgagat gttgcctgcc tttttcagta aatccaagga catgaatggc
    23521 ttctggcaag acattttctc tagcctgcac caaaatatag gtcaataggc gtctgacacg
    23581 cgccttggtg taacgtttgg tagtcactaa ctcgaccaat tcttccacag actgggctgt
    23641 tttaatagct tctttaatgc gcacagccat ttcttgattg acctgataga tggtggttag
    23701 gtcgggattt gacaagattt gatagcggag caagggaaaa tagtcttccc agctcacctt
    23761 actggcctgc tcaaataggg caacagaagg cataaaacgt tctaagaaat cttggtcctt
    23821 ctgatgttga cggagggctg tcgccgaggc aaagtccaca tctttattca cagaatggta
    23881 acctgccccc tgacgctgaa tcggatgcag cttgatattt cgtcctgcaa ccgcctttgc
    23941 ataggccaga gcaagaacat gattgggggt attacctgaa aaatcaagac cagcaaattc
    24001 cttccacata gcttgggttt tctggggata ggaaagggaa tcaggcagat tttccacaaa
    24061 tttctccatc tcagtacctt tctctgtgta taagtcagcg attttctgat aatctcgaac
    24121 ttcctctgtc ccaaaaacta gactatcaat gcccaagcga tccaagatat ccacagctcc
    24181 ttgaccaaag aaatcagctg cctgaacact gactaaaaag ggcaattcca ctaccaagtc
    24241 tgcgccattt tccagcgcca tctgggccct tgtccactta tccacaatag caggctctcc
    24301 acgctgcatg aaattccctg acatggcaac aattttcagt ccctctgcct gatccagcag
    24361 gtatttatgg ccattatgaa aaggattgaa ctctgcgata atacctgtga tggtcatgat
    24421 gttctcctac ttctgcgcca caaaaaacca acgggtgcta gtttctgttg gttccttatc
    24481 ctcaaagtct gcatagagtt tgaaggactt aaatccagcc tgttccagca agatatcata
    24541 ggtcaagatc tcataggtcc tctcctcatg tacttcatcg tggcgactaa aggaaccgtc
    24601 agcctccttg ataaagaaag tcagctcatg cacgatggag tgaggagctt cgtcctcata
    24661 tgtatcccaa agcatggcaa aatcttccgc attttcatgg taggaatagc cagggaatac
    24721 ttcatctgtc tggtaggtcg agtgcacgtc aaagatgaaa actccctctt cattcagcgc
    24781 attgtacacg tccttaaaga cgtcccctac ttccacctca tcctgcatat agcagataga
    24841 gtccgaataa cacgtgacaa aatcgtattt ccctgccttg gacaaattca gcatattacc
    24901 ttctataaaa gtaatctttt gcttggctga agttgctctc ttctccgcaa tcttcaacat
    24961 atccgcactc aagtcaagtc cagtcacatc aaaaccagcc tgagagaagc gcactgattg
    25021 aattcctgtc ccacaagcca attccaagag tttctttctc tccttggtct taggcaaatg
    25081 acgcagagaa aaattcgtcc atttgtcgta taaactatcg tccatgaccg catcatagac
    25141 cgccgcaaag gtttcataag tcgccataat catgatacta agagctccat ggtaaacacc
    25201 actagccctt tacctttcta tcaatttttc taaaataagc aaagaaaccc agttgactac
    25261 aactgagttc atcttctaag caagagcttc tgaaatatct actgaattcg cctcatgcca
    25321 tagcttctct aggttataat gggcacgcat ttcttctgaa aagatatgca cgacaacagc
    25381 accgaggtcc agtaagaccc agcctccagc tgcatcgcct tcgacatggc tacctttaaa
    25441 gcctgcttga gctacttttt cacggatatt agctgcgata gcgtccaact gacggctatt
    25501 cattgagcta gtgatgacaa agtagtccgt cacactagtc aaatcttgta catcaagtgc
    25561 gaggatatcc tccgcacgtt tctcatcagc cgctttcacg actagttcta gtaattcttt
    25621 ttcgttcatt taatcctctt tttagaaaat atgtttgtaa tctaggacat ctgcaaagtg
    25681 ctcctcccaa atgtcctcat aaaattcatt aatcgtttct gctggaatgt catccccaac
    25741 aaaggttgtg acagcacctt ctggaacaat aagctgatag ccatattcaa aggcaacctt
    25801 gacagaggta tccacacaat attctgtctg cataccacat aaaactaatt tttcaatccc
    25861 ctgcttatcc aagtattctt ttaagccagt ttctttgaaa atactgttat acttcttctg
    25921 aaagaccttt tcagcaggtt ttcgatttaa aagcgcagat aactgccaat cttctgatgt
    25981 ttgagcttca gagttctcaa tatgttgaac atagataatt tcaatattct tgcttctagc
    26041 ctggttttgt aaataagaaa tttttcccaa tagacttttt gtctggaacc cagtttctac
    26101 taaaacattc tgaacatcaa tgattataaa agccgtcttc atttagttct ctttcaaata
    26161 gtgcacaaag gcgttatagg tttcaagggt ttggggatag atggggaatc cctgatgagc
    26221 tagatactcc acggtacgag ctgtttcgta ggccaccgcc ttattgagcg atagacttgc
    26281 aatctcacgc gccacatcta ctccaggaaa ggatcgattg tgctcgatat agtctgcgac
    26341 gtagatgacc ttatctaggt ctgtcatctg accagcaccg actgtatgga cttcaatggc
    26401 tcgcaggatt tctgaatcat gcaaatccaa atcttcctga attttgtaaa ttccgaccat
    26461 accatgccag acattattac cccagttttt gaggtcgaaa tctagctgat aacggtcaat
    26521 caagtctaga aattcctgat ctgacaactt tttagcatag tcatgaagaa gacctgctag
    26581 acttgctttc tcaacatcta caccaaagcg ctgagctaat tccatagcgg cacgctccac
    26641 acctagacaa tgggttagac gtttctcagg tagaagctct gccatttttt ccaacaaggc
    26701 ctcacgagag cagttgatat agtcttgata ggccatcagt agagcccctc cttctcgata
    26761 tagtctagca caggctgagg taggagaaag ttgggtttcc gaccttgggc aaggaaggca
    26821 cgcaccatgc tggacgagat atccatgagc ggtacgtcca cccagataac tggataggaa
    26881 gttcctgcct tgtagcgtgg acgctgaacc cccacaaact gaaccatgtc aaccagttca
    26941 tcaattcggt accacttagg caaatagtca accatgtcgg caccgatgat aaagtaataa
    27001 tccgtatctg gatgttgctc tgtcaagatt ttcatggtgt cgtaggtgta ggaaataccc
    27061 ttgcgctcca actcaatggt ttcaatgact aggccgtcaa tcccctcaat tgccaactca
    27121 agcatcttga gacgatggtg ttcagggatg gtttcctttt tatcaacgtg aggaggttgg
    27181 tattcaggca tgagcagaac ttgatccagt cccaactgtt gccgtacttg atccgcaaca
    27241 atgagatggg cattgtgaac agggttaaaa ttccccccta aaatcccaac ttgtttgcgt
    27301 tttttctcct tgatttctgg ctccaactct accttggtaa agggagtcaa tagttcgatt
    27361 gtcataggct agtccccttt atttctctat aaaaacagtt atttggagtt tcggtcttag
    27421 atttctttga ctttcttaga aatcttgcga ttttctttct tgctagattg tttaaacaaa
    27481 atcaagatgc gtcctatttt ttggactgta tccacaccga tttcttcttc caaaatttca
    27541 gctacttcgt ggatgttttc atctgtgttt tgtaagagag taaccttgat taattcacgc
    27601 gcatcaagag cttgacggac gctggttttg atttggtcgt tgagtccatt tttcccgatt
    27661 tggatgatag gtttgagggt gtgtgcctgg ctgttgagga aggcacgttg ttttgatgtt
    27721 aatgacataa ttttcttcct tgtttttgga tagttatttt aggtggtgag ggacggtcgg
    27781 aactaatttt ccaaagattt catctttgaa aaatggattt cctgacctca caattgagcc
    27841 ttggtctcaa ttgtgcccct tgccaatcta cagattggca atcacaccac ggcaataact
    27901 atcgctatat gagctcactt tgttcgctca gcgattttgt tttaaataat tgcttttcgt
    27961 gtgacgacgg cgacgccttc tggtgcccag acggcgactt ttgctatgcc tgttacacga
    28021 atccagccta ggcctgaaat gactaggtct gtcttatctt taatggtaaa gacatgctgg
    28081 actagccttg ggaaatcttc tttttccttg ctatttggtg gtgtcagaag agttcccagg
    28141 tgcttatcgt agaaagcact agctccttca agcttgctac gatggagttt gagttcatta
    28201 tcaaagaaag cagtaaatcc ttgcttttct cctgctataa agtcaaagcg tcccaaacca
    28261 cctaaaaata gggtttgctc aggattaagc tgataggtct taggcttgat ttccttttta
    28321 ggactgacat acttgaggtt tttggccgtc aaatagtgag ccatctggtg gcggtggata
    28381 attcccggcg tatcgtaaat ataagatccg tcgtcaagcg gaatctctat tttgtccaag
    28441 gttgtccctg ggaagcgtga agtagtgatg acattctgat cacccgtgat ttcttggata
    28501 atagcattga ttagagttga ttttccaacg ttggtcacac cgaccacata gacatcgcgg
    28561 cccttacggt agtgttcaat cttatcaatg acttccttaa tggcatgttt attttgtgct
    28621 gaagttagga ccacatcgac tggacgaaga ccttcttcat gggcacgttt catgagccac
    28681 tggctaatct taccagactt aactgactta ggaaggatat cttttttatt tcctaccaag
    28741 aggacatcat tgcccgagac gaaacgtggt aaacctggga tgacagatcc attaaaatca
    28801 aagatatcaa tgacattgac cactaaggca tcactgtctc ccacctcgtg caagagcttg
    28861 aggaaatcat cgttcgtcaa ctggacatct gtgatttcat tgtagtggcg gagacggaaa
    28921 cagcgttggc aatagacttc gccagtctcc aaaccttttt caagtgccga ctggggggta
    28981 aaaccaagac cagctttatc tgtcgtctga atagttgctc cacaaccaat acagagaatt
    29041 tcttccatag ttaaattcct tttttatatg taatcggtcc gtacttttca gtgatttttc
    29101 tcataacacg acgctcacga gttcggttaa tctgcgtttt gattgagtca tgttggacca
    29161 agggtttgac taaaattgac cgaatccctg cacggtgggc tgctcgtata tctgtcatga
    29221 gctggtcgcc aaccatgacc acttcctttt tgtcatagtg gaattccttc atggcacggt
    29281 caataccaaa tgtgaagggc ttcaaggccc agtaaacgta atcaatccca aatttctcaa
    29341 ctgctcgttg aacgcgtttt ttggtgttat ttgacactac gataatgcca atacccgcgt
    29401 cccgaaggtc atgtagccat tgcttcatct ctggcgttcc atcagggttg ttccaagcaa
    29461 tgagggtatt atccaaatcg accaaaacag ccttgattcc ctgcgcctgc aggcttggga
    29521 ctgtcagatc atagactgct tccacagcaa aatctggtat ataattttca atcgccattc
    29581 tggctccttt tcttaacttt ttttcgcaat tttccttagt aacaaggata aaacaatcca
    29641 caaacctgca ctagccaaac tgatatagag ccaagcatgg ggctcatctg ttaaaggtaa
    29701 gggaacattc attccgaaaa agcctgtcac gactgccaaa acagctagca agactgaaat
    29761 gatagtcaag gttgtcaaat tgtcattcag attattgttt agaatattgt tgtaagaggc
    29821 tgaaagctgc tgtaaaatct gagagattag gtctgtcatg gataccagct gatgagcctc
    29881 aatcatggca tcatcaaact gttctctctc aatctcatca aaactacgat acaaggcatg
    29941 accttgaata tgctctaaca aaatccgatt ttgtttggca gctgccgtca gataaaccat
    30001 accagtctcc aaatcagaca ggacaaagag gtttttcttg gtagttcgct ggcgcaagag
    30061 gtcattgacc tcatccctac tcttgtccat ctgctcaatg acaggatagt aggcattgct
    30121 gataatttcc agactggcaa agagaaactt ataaatcgaa agcgtgtcat ggttctccag
    30181 ataacgagtc atctgttcaa tgacataggc gttcttggta ttactaatgg taatcaggcg
    30241 acgatgctcg acaataaagg tcatgggaaa agtctcatag taggccttgt ccttttttac
    30301 gtctaggaca ttatagataa aggtaaccgt cccactttca cggtggtagt ccatgtgggc
    30361 gcgctcgttt ctatccagtg cgtattcaat ggtttccttg tccaaaccgt aaatttcaga
    30421 aaggtcttct agtttattca actttcctag gtctaggtct atccaggtac aaccattgcc
    30481 caactgcttt tctaaaacca tcttttctcc ttattaaact ccctctattg taccacaaat
    30541 tacttaaaat aacaggtcta gtctgtgcgt gagaaattgc taacaactgt gatatttcgg
    30601 ttagggacaa agtgaaggac ttgctcctcg ctacgaagct gtgtcgtacg aattcccaca
    30661 ctgacaacct tacccgatac agtaatcggt ccatttgtca gaacgacctc atctcccaca
    30721 tccagttgac gttcaaagag gatgaaaaag ccattgatga catcagacag aaagccttgg
    30781 gctcccatac caatcgctac cccagcaata ccagctccag ccagcaaact agaaactggc
    30841 aaacctaaaa tcgacaaaat gcagtatagt aaaaagaaat atagcgtata attaaacaca
    30901 ttttctagta aacgtgagat ggttttttgg cgtccgacat catgacgaga catttttaga
    30961 gaaggtttga caattctctg caccatggta tgaagcattt ttttagcaat ataaaataca
    31021 atcaaaagca gtaaaagaga aatgaccttg gttagaatat tctcgataat ggttgtcaca
    31081 tctagctttt caatataagc ctgaataaat ttttgcatat aaaactcctt tatttcatta
    31141 taccatattt tcatcagtct cattctccat aaatattcaa aaaatagaca gaaaattctt
    31201 gattttgtct atcatttata ctataatcat aatcactaca caaaaaggag attattatga
    31261 aaagaatcat tcatgcttgg aataaggcaa gccttgtcaa gcgtatcttg attggtatgc
    31321 tttatgggga atcctaggac tgacccttcc taatctctca gagattgggc tactcgggga
    31381 tttattcgtt ggaggtctaa aagctgttgc tcctatccta gtctttgccc tcgttgccaa
    31441 tgccctttcc caacatcaaa agggacaaga tagcactatg aaaactgttg ttttcttgta
    31501 tatcctatga tagggacttt cgccgctgct cttgtagctg tactagcaag tttcatcgtc
    31561 cctattgaaa ttaccctaaa tagtgccaat actgaaattg caccaccaga tgggattggg
    31621 caggtcctca gcaacctctt gctcaaactg gttgacagcc cagtcaacgc cttgcttact
    31681 gctaactata ttggaatctt atcttgggca gtcatttttg gaatcgctat gagagaagcc
    31741 agtaaaaata gtaaagaatt gctaaaaact attgctgacg tgacttctaa aattgtcgaa
    31801 tggatcatca atctagctcc atttggaatc cttggtcttg tttttaaaac catttctgac
    31861 aagggagtcg gaagccttgc caactacggt attttattgg ttccattagt aacgactatg
    31921 ctttttgttg cccctgtggt caaccctttg attgccttct tctttatgag acgcaatcct
    31981 taccctctag tttggaactg cctccgtgtc agcggtgtga cagacttttt cacttgtagt
    32041 tctgcgacta acattcctgt caacatgaaa ctctgccatg accttggact caacccagat
    32101 acctattctg tttctatccc actcggttct actatcaata tggctggagt agcgattacc
    32161 attaacgttt tgacccttgt tacagttaac actcttggaa ttcctgttga ctttgccaca
    32221 gcctttgtcc tcagtgtggt agcagctatc tcagcctgtg gtgcttcagg tattgccgga
    32281 ggttccctcc ttcttatccc agttgcttgt agccttttcg gtatttctaa cgatattgcc
    32341 atacaagttg ttggggttgg ttttgtgatt ggtgtcatcc aagactcatg tgaaacagcc
    32401 cttaactctt ctacagatgt cctctttacc gccgttgccg aatacgcagc aacccgtaaa
    32461 aaataactca tcaaggcaag cctgctcatg tcttgtcttt tacgctttta ttctaactta
    32521 ttaggaaatt cttatgtcta ttagccaacg tacgaccaag ctcatcttag ctacctgtct
    32581 tgcctgcctg cttgcttatt ttctcaatct ttcgtcagca gtttcggctg gaattatcgc
    32641 tctcttgagc ctatctgata cgcgtagaag tactttaaaa ctggctcgca atcgtctttt
    32701 ttctatgctt ctagctctgg ctatcggtgt tctagctttt cacttgagcg gatttcatat
    32761 ctggagtctc ggcctctatc tggccttcta cgttccttta gcctacaaga tgggctggga
    32821 aattggcatc acaccaagca ctgttttggt tagccatctc ttggttcaag agtcaacctc
    32881 tccagacctt ctagtcaatg aattccttct ctttgctatt ggtacaggat ttgccttgct
    32941 tgttaatctc tatatgcctt cacgagaaga ggaaatccag cactaccaca cgctggtgga
    33001 agaaaagtta aaagatatcc tccagcgctt caaatactat ttatccagag gagacggacg
    33061 caaccgagca cagctggtag cagaattaga cacgcttttg aaagaagccc tcagactggt
    33121 ctatttggat cactctgacc acctctttca ccagaccgac taccatatcc actactttga
    33181 gatgagacaa cgacaaagtc gtatcctgag aaacatggcc caacagatta acacttgtca
    33241 ccttgccgcc agtgaaagcc tgatcttagc gcaactcttt tcaaaaattg caggtcaact
    33301 gagccagacc aatcctgctt ctgatttgct agatgaaatt gaacgttatc tggaagtctt
    33361 ccggaaccgc agtctgccca agacaagaga agaatttgaa acccgcgcca cccttcttca
    33421 actcctacgt gaagccaaaa ccttcatcca agtaaaagtt gatttttacc aaaaatatag
    33481 acagtaatac tcttcaaact acgtcagctt caccttgctg tatatatgtt actgacttcg
    33541 tcagttctat ccacaacctc aaaacagtgt tttgagctga cttcgtcagt tctatccaca
    33601 acctcaaaac ggtgttttga gctgacttcg tcagttctat ctacaacctc aaaacggtgt
    33661 tttgagcagc ctgcggctag cttcctagtt tgctctttga ttttcattga gtataaaaaa
    33721 gaaaacttca gaccaatcca caaaaggtga atatctgaag ttttctttta tttataagta
    33781 atcaccataa agattaatag tagaacaaat aaagccaaac tcaccaataa agtgagcacc
    33841 ttgaccaagg tgttgctctg ccagtagctt ggtttaccaa tattactagt ggcatccatg
    33901 gaaaatagtt gattttgacg gggttgtata gttaccaata caatcaaagc aagagatagg
    33961 ataatagcta aaacaattag tatttcaatc atagatctac ctcaaaattc ccagcaaagt
    34021 aaagagcaaa ccaatcagaa tcatcaaacc cagtaaaagt gaaaaagtct tactaatctt
    34081 ttcccctcgg gtttcttttt ccttcttgat gggtttttgt ttcaactctt ccatgcggcc
    34141 ttcaacgtct ttgtaaaata aatcttcctc tgacatgtta gcctcctaaa acagtttgca
    34201 tggtttcctg atatctcacc aagtcaacat aatttgcatg ttggccttgt ttgccaagtg
    34261 cctgacgcat ttgatcaaca tctgaaaggg ccaatttaat ggtctcaacc aaagcagcaa
    34321 cttcactact ttcaaataga tggtctggag cgatataaag tctattgtga accgtttgat
    34381 taaatccaag aatcaagaga ttgtgctcaa aggcctgacg aactgcctgc aataactcat
    34441 tgctatagtt gatatccaag taaatatccg acagttgata caactcctga atcttctgtg
    34501 gactagcatt ctggtaaaga accacattag gatagcgaag catgtctaag agcttagaag
    34561 acatctctgt caccgctgcg atacggaagg taacatcagg caaggcttct acgattgctt
    34621 ctacttgctc aatctgatct gaattggtca agattaaggc gtctcgtctt aggaaattat
    34681 cacgtttgaa ctggtaatga taacccaagt gcacaaactg atcatggtat ttctcgtcag
    34741 ttaactctaa agcgcgctca taagtcgtct tatttggaat gatgatcttc ttagtacgca
    34801 cattatcact ttccaaaatc aactgcatat tacctggaat ggcatcatag agaggttcct
    34861 gccataccaa gacatccgag ccagatttat ctggatgatg gaaggaaacc aagaaaggag
    34921 tcgctagagt attaaagata agctgactgg tgtctatttc caaatcttgc aaaaagaagg
    34981 tgataaattc aactttattt gcaaagtaac gcatggactg accaggcaaa gtcaataaga
    35041 tatcacccgt cacatggttt tccagtaaaa cttgttggcc attgacatct tggtaaactg
    35101 tcataatcgg ctcgctatct gcgctataag tggttttagc aaagcaagca ccaaagcgat
    35161 tgtagtggtc aacctgacgt actcgacctt ctaggtcttt ccagtctacc tgtttaacca
    35221 agcgagcctg cattccatca gcataatgaa tgacagccct ctcctgcgtc acatcttcaa
    35281 tacaagcaga ctgattatct cctaaaattt cccaaaaatc tgaaacggga acttggttaa
    35341 aatagagggg ttttccatcc tcgtaaccta gataataggt aaaaggagaa agcagaccat
    35401 caggcagaaa accatctgca tcgatgacca ctccaagttg agaaagacca gtagcgacta
    35461 gactttcatg taaatctcga ctttcctgac tgtaactgtc ataaagttca atcatggagc
    35521 acctcctcta ctgttttctt ccacttttct aaaatttctt tggtcaagaa gccttctgca
    35581 atttggtaag aataggcacg catagcttcc aaacgacttt cttgatacaa ttgacaaatc
    35641 ttagcggcat aagcttgctt gatttggtct tctacatggt cagatgaact tggaatcaaa
    35701 taaccatttt gcccatcctc tataaaggtc tgattaccat aaggcacatc aaaaccaatt
    35761 agaggtagac ctgaaccaat agcttccatc aaggtcagac caaatccttc gctggtagaa
    35821 gccgttaagt agacctcata ctggctataa atctgcgaaa gttccgcatg ccccttgagt
    35881 tggatatagt cctctgcctg atgatttgca ataatttctc taagcagaga atcttctcca
    35941 ccactaccat agatatcaaa ggttagttcc ggtaactcct tatgagcttc aatcacagct
    36001 ttcacaagcc aatcaatgtg tttttctttg gccagacgtg aagcggtaat taaagaaaat
    36061 ggcttacgtc cctgacttga ctccgtcaag gaatcaatac tacctacagg aatcgtaaca
    36121 atctttggtt gatgcggggt gtatttggca aattgctctt gcagaacttc tctctgtcta
    36181 tcagttgata cgataaagaa atcaacctta ttcgcattgg taaactggta gtcgtaatag
    36241 ttgttccaaa ggatatagtc ctcgtttgta gcattttcac tataatgctc cgcatgaaca
    36301 actaccgcta aatgtgctgt ctgtgcttcc tcaaacacaa cctgaccaat gcctgtctcc
    36361 ctatcgagaa tgaccaaatc agacttattc aaattcaaag atttcataaa ggcacgcaca
    36421 aaagcttgct ttccatagaa aatcttatcc ttgaaatgat aaacttcttc cttcccttga
    36481 ttcatcaaaa tatcatagac tggagtcccg tcttcattgt aaaaagttcg ttggtataag
    36541 actgcaacat tgtccttggg agcaaaatac tcgctacaat aacgcgtata agaaaagtaa
    36601 tccttccgaa tcaggtttcc cttaaaaaca tactcggcat gttgaaccaa gtccttgttc
    36661 tcatcaacca aataacaggt tacaaactta tcttggtcaa aaaagaatac acgtaaaacc
    36721 ttgccatttt tttctctgtg actttcttca ccaccaaagt aagccaagac atcatccact
    36781 gtcacgctag taggtgcaat tttgatatct gtgaaatgat tataaagcca gataacctga
    36841 ttatcatcaa aaccaatatt ggctgttaag tgctgaatat tatcggctaa aatcatatct
    36901 gtaaagataa acttagagga cagatttaat ttccgaaaaa caccagcacg ataggcttga
    36961 gcgtattcaa caccgctact agcccaacca attcctaaat ttatattgta aattgtcata
    37021 ttttctcctc ttatggaaaa tgagtcacga tttcaccttt tggagtgacc tcaaccagcc
    37081 ccatcaggag attacgcacc atatccgcac ggatttgttc tttcatagct tcaaagcccg
    37141 cgtaggcttc ttgatagtac tctacgattg gatttttctg actagcagat tgaccaccga
    37201 tagccatgga tagctgttgt agatagtcta cctgctctac ccagttgtca tcaatggctt
    37261 taagcagtga aagtcgtaaa aactgttcat ataagtcatg ttgattaagt aattctttct
    37321 tttcagaaag ttctttatca atcacctgct tcataaagct acgaactgca gttttgtcag
    37381 ttacatctat ataatctgga acctctttaa catgaaaact aatattggtc acaataaagt
    37441 gaaacagtaa ttcacgacta gcatagtgat cagccgctac ctcttctgta tatctctcaa
    37501 tgatatccac aacaacatcc tctaagtcac gagaaccatc tattagacga ttacgctcct
    37561 tgtaaatcat atcccgttga atattcatac tttcagcata ttctagagtc tgacgacgtg
    37621 ccgaacggcc agcactatca ctagcatgtt gagccttttc gactagtttc cggtatttac
    37681 gacctttcaa tacttccggt tgagtcatat cttgaacctg atagtctttg tactttttat
    37741 gaacccaaga tggaccaaat ttcttgataa catcatcctc taaggataca aaaaatttac
    37801 tcataccagg atctccctga cgaccagaac ggccacgaat ttgtaggtcg atccgctgac
    37861 tttccatccg ctcagtccca ataacaatca agcccccaag ctctgcgact cctttaccaa
    37921 gcttgatatc cgtaccacgt cctgccatag aggtagccac tgtcacagcc cccatctgac
    37981 ctgactcgga gataatctga gcctcacgcg ccgcattatt agcatttagg acattatggg
    38041 caatcccttc acgaaacaag agagacgaat agagttgaga catttcaact gagcctacaa
    38101 aaacgagtaa aggatttccc ttagcatggt attccttgat gtactccaag gatgcataca
    38161 ctttttcagg taaagtgata tatagattat ctggatagtc aatccgttgt ctcggacgat
    38221 tggttggaat gcgtactaca gacatattgt aagtttcaat aaactctttt tccgcgacct
    38281 tacctgtccc tgtcatacca gatatcttat taaacatctt aaaaagactc tgataggtga
    38341 tcgatgccat agcccgcgtc tcaggagata atttgacatg ttccttggct tcaatagcct
    38401 gatggagacc tccttgaagt ttagtcattt ccattagacg ccctgttccc ttatcaacca
    38461 gtagcatctc atttccacga atgatatagt ccttatcttt agtaaagagc ttatgagctc
    38521 gaatcgcata aaccaaatga cgagcaaaag acgcatgctc ttccttgtat aaattatcaa
    38581 tccctaggaa attctcagca gacttggccc ccttagtagt gagccaaacc tcctctttct
    38641 cctctttaaa gatataatcc tctccttcga ccaaggttgt tacaagtgta tcaatgatcg
    38701 catagtaatt agactgaaca cgaggagaac ccgcaataat cagaggagtt tgtgcactat
    38761 caagcaagat atcatcaatt tcatcaataa tcacatagtt aaacggtcgt aaaaacttac
    38821 cttcttcatt cgaggctagg ttatcattta gataatcaaa acctaaatta ctattggttg
    38881 tgtagatgat atccgaagca tagataagct tcttttcttc agctttcatt tccttctttg
    38941 gatcttccgt aaatggtaca ccaatggtca atcctagaaa acgataaact tgacccattt
    39001 cctcggcatc acgctttgat aaatactcat taggagtcac aaccatcact ccttctcctg
    39061 aaaaagcgtt caaatagaca ggcatggtag ctgtcaaggt cttaccttcc cccgtattca
    39121 tctcagcaac atttccatag tgcatgacaa tagctcccat gacttgaaca tcatagggga
    39181 acatccctaa aatccgctta tctgcttcac gtaccacagc aaaagcttca accaaaatat
    39241 cgtctagact ttctccctcg gaaagacgct gacgaaactc tactgtttta gctactaatt
    39301 cttgatccga aagagaggac atcttgcctt tcagggcatt aatctgcttt aaaatctttt
    39361 tcactttcct aagctggaaa tcttgaccta aacgtctaaa cacttctgat ctccttgata
    39421 gaaaaatgat aaaaagttaa ggactcaaat ccagcactta aaagagaaac ttgataagta
    39481 taactatctt ctggataagt aaatatgaaa tctttcgctt tttctaccct tttttctaaa
    39541 atatcaccat agcggtcaaa aaaagttatc ttggtataaa ctccattttc aggctgacaa
    39601 tcaaaaacca gtgatagttg atactcctgc tttcttttta ataagggaag ctgaggttgg
    39661 gttcgtgcag cctggtaatt gacactagaa aaccaagtct ttagagtctc accagatggc
    39721 aatagaggat tatacagacg aacacttttg tcaaggtaat aagatacagt tgttccatac
    39781 atataagtcc caccaacttc tccccaataa atagactgtc tctgtgtaat aatcatcttt
    39841 tctcccttcc aaaaccagtc tctaagacca ttctataaaa gtgcaaaaac caagaaatat
    39901 tggtatctgt atcatcattg tgtcgtccag cagttccttt agataaaatc ttcgcacctg
    39961 tattacacag ataagacact aattgttcat aggcttgtgg atccatttct tcatctttca
    40021 tataggataa accaaaggtt gtctttgaaa aatttgcttg tttaaaaatt ttccaaaaac
    40081 gctgatccaa ctccgtcata tcttgagaac ttgtcccacc tgtatgatga attaaacaat
    40141 caaaagctaa gttagagaca cccggtgcgt ccaaacgtcc acgactagct atagttccta
    40201 aattagccaa gggcttacca actatgatgg catggggttc aaaagaagca ccataataga
    40261 gagccggaaa cgttcccata gaaagacctg ataaaatcaa atccttatga tcaaagccta
    40321 aataatcaag atagtgagtg atcgtatcct ttacttttcc ctctagctca tccgttccga
    40381 gataaaaagc tcccccctct aaacgtggat cagaaaataa aatgaaggga catccaagag
    40441 ttttcatcat aaagtaaccc tcgaatcctt ctgcaggtcg ataacctgca aaatagacag
    40501 tcaaaggcgg tttaaaatca cctggatgga agaaatagtt tatttcatca cgcttggaat
    40561 catgtaggat atttccacca agtacaaact taccaaattg ttttcgactc catcgttggt
    40621 gaagattacc tagtttgata gttccacgac ctcttgcttc aacagatatc gagagaaagt
    40681 agtctccgtc tcgactatcc ataaaaagtg cttgctccaa gtctttttca gatactatta
    40741 cttcttcaaa gaactcatca acagacccac tccacatttt acggattact aaacgaaagt
    40801 cacaattgcc ttccttctca tattcaagcc acaactcaat agggagtgtt ttttgcacca
    40861 taatattata agcccaatag gcaagctggg caaaatcttg cccaaactca ccttccaaac
    40921 tgacataatc caatccttga taagaaatag ctcctgtaaa atttggattg acttgtatcg
    40981 tcggaggaaa gagtttatca ccatacccac cgccaaaaag agatttactt aaatcattga
    41041 ttagttgttg gggacttaaa aaatcaattg cttgtgcaca tcgttttttt agaaaatcca
    41101 caacctctct atcatttatt tgtagattct ggttatagaa caaagtataa ggttcaaaat
    41161 attttacata ggaaaataag tctttagcat actgaccatc ttctattaaa actgcatgaa
    41221 aacggttaat atcttctttt tcaattattt cacgaagggc ttttgatgag tttgggtaaa
    41281 aataatacca actcatattt tcaggtatgt catagtgatt ttcccaattg gtaagcccta
    41341 cctgtaaaat attaagttcc tttgacattt ttaacctctt ctatccattt ctctaatttt
    41401 ccaagaaatt gttgtccagt gtgctcctta atcttatcaa tcgaataaat taaagcctca
    41461 ttccactctt tcaacctatc agtgtaataa tgtgcagcct tagaaaattc tgtcacatct
    41521 gtcagcaaat aaccattttt taaatgttcc acatagacgg tttcaactag gttgatttga
    41581 ggaattcctg cactaatccc agctatctgt gtatagagat gaggatgact atttaaatcc
    41641 acaatcaaac ggacaaattc tagtgttttt atcaaatcta actcatcatt caagttgaca
    41701 aactgtatac gtaagtcctg gtgttgattt tcttccagag gattttctgc atcaccataa
    41761 tcaatcgcct ttcccagatt ttcggattga atgttttctt ggatgaaaga ctcaacaatc
    41821 ccttcaacca cattcatttg ctcctgacta gcagcaaaag ctccaaaaat cacctcagta
    41881 tccttatttt cggcgacaaa ggacaagact tgaagtagag cttgattatc aatcccctgt
    41941 tcaaaatcca gttgataata tatgatggat tctttcttag tttggcttcg tcccaatcgt
    42001 aatcgggtgt caaaagatga taaatgataa cattgatgca ttcgttcagg atacaactcc
    42061 tgcaacaatc gtaaactatc ctctctatcc accaaaatca aatccgattt ttcaaaagtt
    42121 acatctaaat ccctaaaggt atcttgagaa ttacgtccaa tgaacagact caacagttta
    42181 ggattagtac taggtaaacg atctagtacc aactggtcat gatgagaatg agaaggaagc
    42241 ataaaaatat cctgatcctt cacatacgtt tgcagataat tctcaaaaaa ttctgccacc
    42301 agatctccca tattttgata ggtaagcatt ttaaagcgat aacccaaaat tggattgact
    42361 tctacctgtc ctccttcttt taaacgctct ctaaattgcc aaactccctt aggattcaaa
    42421 tattcttgat aggctgcttg cccatcttca aaaaaaataa tactcgatat aaaccctcga
    42481 tcatccatga tatggtgcat atgtatttga ccattcggtc caaaatactg tatatcactg
    42541 atgaaacctt caactccatg ttcaacctta gcaaatttct tcccattttt ttgaacgata
    42601 atcgtaaagg gactataaat aaattcacag tcatcatccc actcaatatc tctaatgctt
    42661 aaaacttggg tatggagatt atgaaaatct tgcataacat caaaaacgga ataagtatcc
    42721 gtttctaaca caccatgtct atataaaaaa taacgtagat gaggctggta agctaatact
    42781 aatagacgag aatctatatc ttgctcttga aagagccgaa tctggtgaaa ggtatcatca
    42841 aattctagac gaaaatgaga aaaataccat ggagtgatat ctgcatgcca tgttctttct
    42901 gacccatacc aagctggaat aaaataatac ataaaagtct cctaaatcaa aggtctatag
    42961 gtctcattta gccttaaagc cttgacctcg tctctaatcg taaaaatcat gcccccgaac
    43021 atcataaata agcctggaat cattgccaat cgtaataact tttcatcaaa caaaacaaaa
    43081 agcatgggac cacctgccat aatcacatta aagagaccac ctatgactga gaaacgaagg
    43141 accaatcgat taataaatcg actagtatcc gcacctggat aaataccata aatgtattct
    43201 ccagattttt tcatacggtc tgcaatctct tctccattca tcgtaacaaa agcaaaaatg
    43261 atactaaata aaaataagac cgaaatataa acatagaccc acaaaggctt cccaagcata
    43321 aattcctttg ataaagccgc tagccctgaa tgattaggga aaataaatcc caacaagatg
    43381 aacaaataag ctggtacact aagaaaactc atcacataca tataaggcat cccacctgca
    43441 ggattcaaca tgatttcgag ataagaatag cgtttaaatc gattgtgtaa gccaatttta
    43501 ttaacaggaa ccaaatagcg agctcgatac ataagggcaa gcaagtaaga aaatataatg
    43561 gtcataaaaa caagcaagac aacaatccct gtagggatat aaaccagttt aaaagtttca
    43621 ataacatcct gagggatatt taaaacaatg ctggatagga ggattgcaat agaaccccca
    43681 attcccatgc tagcatttaa atctgacaac caaacaagaa aaaatgttcc tgctatgagc
    43741 aacaaggtat tcattagagc aaccaaagtt accgagtagg aggattgtac tggcaagctc
    43801 aagctaactg ccaaggattg aatcacagct atcatcaagg ccaggtacat tttacggcga
    43861 tcttgtattt ctatagacgt agatgttaaa cccaaccgtt tagaaaaaga aaacatctgc
    43921 cacaaaatca tagctgacat ccaaggggat aatccaacag aaaaaattga caaacttctt
    43981 agatttccac ctgttagggc ggctgagaag gctagatagg ctgttgaacc tcctaaaaaa
    44041 tctttagtat ttaggtcaac aaaagggaga gtaatacgac ttcccaatac atagataaac
    44101 agtagaaata aggtaaatag tccttttttt actgctattg acgaataaat cttagtcata
    44161 aaacactcct ctcaccttca ttgtttgtga tatggtaaat aacaattctt ttatttcaac
    44221 ttttaccatt tctttgttct gcatcaaaaa acaactttaa tctgaaacaa atatctttat
    44281 attcctcagt atctgttaat tgatggtatt ctaaagtttg cttataatct tttaaaagat
    44341 tgacaaatcg aatacgcaga acggagacat ccaaaccagc caagatacaa tccgatattt
    44401 tcttagaaaa cacttcaaca atatctcttg cccaggaaag atcaaattcc gttctcatga
    44461 tgcttcctga acgtcttcta tacagataga gattatcgtt tataaagacg attttagagg
    44521 ctaaaagata aaagcgatgc atagttgctt catcatcaaa gcggcgacca tttgaaaagt
    44581 gaacttcatt gaataattct tttttaatca acttaaatgt cggcaatata aaggccgagc
    44641 tattgaattt ccaatctcct gcttgacggt tcataatttc ttgtatagct aattcttcga
    44701 cgcaaaaatc ttgccctgtt acataaaaat aaaaattccc gtcactttcg tcataattat
    44761 agtaattccc tataacaaca tctgcatcct tttcttggac ttgctgataa agagtttcaa
    44821 tcatattttc ttttacgata tcatctgaat caacaatcgt gatgtactct cccttagcta
    44881 cactaatccc catattttta gcttgtgctg cccccccctt attttcttga tgcaatactc
    44941 gcacacgatt atcttgcatc ataaaagcat cacaaatttc cccagaatta tccgtagaac
    45001 cgtcatcaac taaaataatt tctatatttt gataggtttg cttcagaata tgctcgacac
    45061 actccactaa atattttccc gtattgtata tcggtaccac aacactaatt aattcactca
    45121 ttactctatc tctatattct tcattctatc cactaaatca tctggtctct ccacagaaaa
    45181 aataccgtca tagagtccat catccatact tttacgtgtg atatcaaaag cgaagatttt
    45241 cttgccactt tcttgtgccc ttgtaactac attaaaaacc tctccacctg tattaatatc
    45301 taaataaact atagaatcgt ccaatagcca atcaattcta ctatgtaata cattttgata
    45361 tactgttaca ttcgtatact gtgatagaga ggtcagctcc tcagaacaat cacacggtgc
    45421 agccaaatga aaatgccaat caggtaaccg ttgtactaaa tactctaaat gttttatatc
    45481 tgcactccat gtcacaagca tcacttgttt tttagactga ttgcttcttt caaaataatt
    45541 taaatcggaa cgttgaaaag caatctctga ccaatccaaa tctctataaa cccaccataa
    45601 ttcacgtagt ctagatatac tatatgtatt ccaaggttta tcatgactag catagtgaac
    45661 aattgtaggt ggattgtcat ctagacgttc acattcttga gcaaggtgat aaatatcaac
    45721 acccaccata taattatatg ttttatctaa cgctagccaa ttatcctcaa aataaatatt
    45781 taaaatactc tgatccccaa gatgaacaac ttgattctgc tcggccgcta attccaataa
    45841 actattgaca atagaatgtt ctttccactt tgcaacatcc attagtaaca taccagtatt
    45901 aaatccagat tttcgtcctt cataggcata gacatcatca acagcaccaa ttgaatatcc
    45961 tttgagatct atctcaaaca aagtagctag ttccccagtt acaatgatat cggaatccag
    46021 atacaatacc ctatcagatt caaccacttc tgtcgcaaaa aatctaaagt aagaagcata
    46081 atttatatga ggtcctgttt tatagctttc aaaaagttct ttatcaatgt gtacactgcg
    46141 aattgtagaa ttcacaactt ccatttttct ccccaataat ttaaaccatt caggagctat
    46201 atcactgttg agaatataaa aatcaacatc tctattgtga tacaatacag actttatagt
    46261 cgtctctaaa ggaataagat aggcattatc tgccgctaat actattgatt ttctcatact
    46321 gttctctacc tcatgtaaga tctgattgct gcaaccattt ttttcggaat ggagcttgga
    46381 tagatacctt catatgaaat ctcagaaaga ttttcagagc aagtattgtc gaaagctatc
    46441 attggttttt tgtacttaag cacaaactca tatgcatctt ctaattttcg atcatgattc
    46501 aaatccaaat acaaatctga agctattact atttgttcca ataacattgg taaggaattt
    46561 ggatatacgg taacattagg aaaacgcgtt aaagctagta gttcatttgc catatttgta
    46621 taagcaacaa tatgaaaatg aacttctggc aattgctccg ccaaataatc gattttctcg
    46681 acacaccaag aattcgttaa attaacacat tgcaacttaa atatttgtga tttactagga
    46741 tacttcacac tctttgaaaa ccattcattt aaaataacag accaatccat caaagagtat
    46801 tcccaccaaa cttctcttag acgtccaaca gaaaattgat tccaaggctt atcctgagaa
    46861 atatagtgta aaattagtgg cagtggttcg agcggaatat caaaaatgaa ttgatgttta
    46921 aaggctgccg ccccataatc atatcctatt tgaaaattat attgatcttc aagggaacta
    46981 tattgatctt taaacaacat attcaaaatt gactggtctc cttcttccac attctcatgt
    47041 tctttttctg ttaagtcaat caatttttgt cgaatagttt cagatcccca ttttttgttg
    47101 ttaatcaaga gaacaccagc attgaagccg actcctgctc caaagcaaga acgagctgct
    47161 gccaaataat tttcacctaa gtctaattca aacaaatcgg tcaaatcacc agtcacaatc
    47221 aaatcactat ctagatagag aactttatct tctgttacaa aatctggaat aaaatagcgt
    47281 gcaaatgtca tatgattgat atgaggtaat ttattagacc aattcatttg aaactgtgag
    47341 ccaattaact tgcagtcaat caagtcgccc cccatctctt gtaaatatat tcttatttga
    47401 ctaaaccatt cctgaggaat gtcctgattt agcagataaa ttttcaaatg actattgtgt
    47461 ctacagagtg acttcatcgc cgtttcgatt tgtcgaatat aagcgtaatc acctgcaaat
    47521 actacagcgc gtttggtatt tctcatttta aatccttatc atttatttta aatagtctag
    47581 tttaatattc gtgcaacaat ctgactgtta taggtttcat catcaataaa ttcaatatct
    47641 tggtcgaaat tatgttctgc agtatgttta tgagaaaatt taaccttagt tttagctccc
    47701 attttcatcc attcatactc tgagtcaata tggcctacat ccaaaacttg atagcccatc
    47761 tggcatagat tataactcag aacttttgct gtaggaccca gcatacataa aatcagtctt
    47821 ccttcagcat gtttcataat ttcctcttga atgttatcga caacggaata agcattatga
    47881 gatggacaaa taatcctttt cacagatttg accttatcaa ataaatcatt tccaacccca
    47941 gagcgagatg taatcccctc tacaatcaga atatccctat tctcccaaat actctttagc
    48001 ttttcaaatt gtccttttgc cttactctta tcttcataat caatgtatgg acgcgagata
    48061 aaggtagagc cataccatga acccttacac aactctttat aaaaatccat ataaacattc
    48121 atatgttctt tccagaatgg tgtagcccac caagtaaaga caaaacgatc gtcaaaagca
    48181 tcaggtaaac aaaccactaa tttctcgtca ctctcaagac ccacaatatg ttttaattgg
    48241 ttagctaact ctagatcatg attttgataa ggtataggca aaccccacat caaattgact
    48301 tctccgtctc caaaacgaac taatgaggac ttatttttga taacataatc cagagtctga
    48361 tcaattccga taaccttgat tggcttctct ggttctccca aagtctctcc ttttatcaat
    48421 gcatgaaatg cttgaagaag cctttctgga tgctcacttg aatacaacct ttggccatta
    48481 ttcccatttt gagatttata aaaggctagt ataggtttag atagatgagc catttctgca
    48541 acgatatcgg atgttgaagt cagtaggttg atatctaaat aagcatccat cttctccttc
    48601 aactcgtcta gcttatctct agaaattgcc ggatagagct gaatattagg ataacgatta
    48661 gacaaatcat ataattttgg ccccatagct gtccaagcag caatttgaaa aacaacattt
    48721 ggcaactgtc cagccaagta atccaattgt tcgatatcct gagaagccgt aaacgttaaa
    48781 caagttaaac ttggaagttg tcgaaaacag gtaagatgtc cgtttttctt aaaatcttta
    48841 atcgtatcaa tcataaagga aactttccca ttttcgaaaa caatttgtcc taactctccg
    48901 tgctgagtcg attgaaaagc tagtagcgtt ttttcttgtt cttgcaaaga ttttaaaaag
    48961 ttctcatctg cactaccatg attgatatcc aaatatagat ttgttgactt tttcagctta
    49021 tctaatacag gaggaacaat ttgtggatgc aatctcacat tattatatac agctaatttt
    49081 tttaatttat ctcccatatc cgtccaagct gcgatatgaa ataccacctc aggtagagct
    49141 gtaactagct cttctattcc ttctaaatct tgggaattcg ttaaggttaa gcaagaaaat
    49201 tccttatcta gaggcgatat tagttcaaat tctcccatat ggtgttgtaa aatctgactc
    49261 cactccaaat catggaattc ccaccataaa tcacgataac gattagctgt caaggtggtc
    49321 cagggttttc tgtaggtcgt aaaatgaatc accactggtt tatcgttaaa agccagatgt
    49381 tcctgccaat tgttatacaa agccacaata tcatgcccta cttgtaaatt ataagctcga
    49441 cctagctcta accaatcatc ttgtaagacc tgattaaaaa tcgtttgatc accattaaaa
    49501 tgctcgaaac ggccttcttc aacttccttc attgtaacaa tgctctgttc aattagtcgt
    49561 tcttttaatt tctcttgacg ccatttttta ttgttgatta atagaacacc tgtattaaat
    49621 gtaattccat ctgtatcttt aactgctgcg agtgattttt cttctaaaca aatactaaat
    49681 aatttctcta aagaagtatt tacaatcaaa tcactgtcta aatataaaac cgtatcttct
    49741 tggatataat ctgcaataaa atatctagca taggtaatgc tactaatgtg atcttgcttt
    49801 tcccaatctt gaaacacagt ttgttcaggt agtttaacat cgataatctc actacctaac
    49861 atgcgagcta tttttcgtgg tttgcgaaac caatctggca taatatcttg atttaaaata
    49921 taaatcttaa catctcgatt gtggtataaa atagatttta tggttgtttc taactgcctg
    49981 atgtaattac gatcaccagc taatacaatt gttttaatca tcttgttctc ttattcaact
    50041 cccaaatggt aaattgcatc taccaataat tttttagtaa aataaccttc ttttagcaaa
    50101 taactaaagg tttttatccg attcgtcatt tcttgatatt cttgtagatt cattttatca
    50161 actatcgcat gaacttcttc tagactattc gccacaaagc ctaagccttg atctactata
    50221 aatttagcag ttgataagtt gctcggtaca atgactggga taccagctgt tagataggta
    50281 ctcaccttat gagatatatt caaggtatag tattggttac tttctccctc attttgatgg
    50341 gttccccaga caaggccaaa tccacccttt gataattcta gcaacaattc ctcatctttt
    50401 ttccatcctt cggtgctgag atttctagca ctagaactag cttccccttt atttgaaaat
    50461 actctcaaag gcgtatcttg agaccaattt tgtaagtctg gaaaacgctc taaacttcca
    50521 gcaaaaaaaa gttctttttt aaaagcaggg gtgtataagg ataaatcatg aggatgatcc
    50581 cacatccctt gaacaagaat cttcttagtc gtcaatcctt cttccatcag gcgtgttttc
    50641 attctctctg acggcactat caaaacatct gatagattat acatatacag atagtctttc
    50701 atgagataat agttactatc aaacatgagg ggaacaacat catggataaa gcaaataatt
    50761 ttcacctgca tatcctttag cttgtcaaac aagagacgat caaattcaaa gccattccag
    50821 gttggagact gaaagactaa gatatcacca atagagatac tggccataat accatccaga
    50881 cgcttattca tttcagaagg actatctgaa gcaatgttgt aaaaataaat accaacctct
    50941 ctaaatccca gttgactagc tatcttttga acagcatttt gagctaagat aaccgtacta
    51001 tcaccagcca tgccgtataa atttgttaaa tgtaatttca tattatgcta tctcattttt
    51061 tattactatc tacggaagta aagacaattt ttccatcatt cttctataaa tctctgtgaa
    51121 ctgcatattt tgttcttcta attttgctat cactctttgt aatctatttt tataaatcaa
    51181 aatatgattg gtcaagtcat agtctgaact tgctaaaata gctatctttt cttccctagc
    51241 cccaatttca tcatacatac gcttttcact ccatgtattc gataaagtat cagataaacc
    51301 aacacggtac cagtaaacac aacgattcaa atagactatc ctcgaagctc ttagaagtac
    51361 cttccatgtc cagtaagtat cctctgcaat ttttcctata ggaaatggta aatcttgtag
    51421 taactctctc ttgaataact tcaagacagc tacagtccag ttcccatttc tgacttcttc
    51481 gacaccttcc ctatccataa ttgctttacc ttctatcact tctagagaat catctggatc
    51541 cgtaacataa gtcatatata catagcgtgt ttcatcataa gaattataac gcccgatact
    51601 aatatctgcg ttttcctttt tcaaagcacc atataatcgg tctaaagcat catgttccaa
    51661 ccaatcatca gagtctacaa aagtaatgta cgccccaccc gaacattcaa tacctaggtt
    51721 acgagctgat gaaagaccgc cgtttgcttt ctcaaaatat ttgaaacgag aatctttctc
    51781 tacaaattct tcacatattt tggatgaatg atctggagag ccatcattga ttaataaaca
    51841 ctcaaaattt tgatacgtct gattctgaat gttatccaaa cacattcgca aataattctc
    51901 aacattgtag attggaacta caatactaat tagttcgtct gccataaact tattctccta
    51961 taaattgtct aatagcctct accattttgt ttggttcggt tgaagaaaaa atttgacttc
    52021 ttcctttact atcatgacta gtattatcaa aaccaaagat tggcttattg cgcctacaga
    52081 cttcttctat aatgttgaca atctctcctt catggttaat atcaagatag aaatccatct
    52141 tatccaaggt ttctttcata ttaaaagaat taaaatttgg aaaaagtgaa agattcagat
    52201 atctctgtaa atcaacaaca gagggagcaa aatttgtatg cgccaagatc gtaaagtgca
    52261 cttctggcaa ttccgatagg agatattcca aggactccat tgcggcacta tttgtaaaaa
    52321 tacaagtatg atataaggga acacccacta actccttaaa agagcgttta acgatatctg
    52381 aacttaacaa cacatcgttc cagttcaatc cataataaaa ccaccagagt ttacgaaaac
    52441 gtgtaagtgt taaatgagtc cacggtttga actctgtagt ataatggaca atcttaggct
    52501 cgtaatttcc ataatagttg gattggtacc agtcagcatt accttttaaa tgtaaaaact
    52561 gatctaaacc aaccatgaaa ttatactctt tgtccaaatg tagccactgg tcatcgaaat
    52621 atatattgag aacaccttgg tccccataga catgttcatg gtgttgagca gtcaactcta
    52681 gcaaattttg tgttgatttt ttttctcgcc atttttttgc atcaatcaat aacatacctg
    52741 aattaaatgt tgttaaccaa ccatcttgat caaaatcttg aacagctgcg atagcgtatc
    52801 cttgcaaatc caaaaaaaat aattcatcca aattggcatt cacaatgata tccgagtcta
    52861 agtagagtac ccgatcttca agcacttcgt ctgctatgaa atacctaaag aaagttgcat
    52921 aggacaggat tgcatttggc aagtggaaat cacgaagatt gtgatctgct tttttgacat
    52981 taataatctc agaaccaata accttcagac gatttctcat caaaagaaac cattcactag
    53041 gcagatcatc attaaacaca taaaatttca cttcgtgatt atgaactgaa actgacttta
    53101 taacggtttc taccttatca agataatgaa tatcagctcc taaagcaata acttttctat
    53161 acattcttat ctcccgattt ttctatttta
//
LOCUS       DAVLVP010000006        43888 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.006, whole genome shotgun sequence.
ACCESSION   DAVLVP010000006 DAVLVP010000000
VERSION     DAVLVP010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 43888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43888
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.006"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(829..2079)
                     /gene="ilvA"
                     /locus_tag="VYN69_000316"
     CDS             complement(829..2079)
                     /gene="ilvA"
                     /locus_tag="VYN69_000316"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HEV1532667.1"
                     /translation="MLSSKDIIKAHKILNGVVVNTPLDYDHYLSEKYGAKIYLKKENA
                     QRVRSFKIRGAYYAISQLSKEERERGVVCASAGNHAQGVAYTCNEMKIPATIFMPITT
                     PQQKIGQVRFFGGDFVTIKLVGDTFDASAKAAQEFTVSENRTFIDPFDDAHVQAGQGT
                     VAYEILEEARKESIDFDAVLVPVGGGGLIAGVSTYIKETSPEIEVIGVEANGARSMKA
                     AFEAGGPVKLKEIDKFADGIAVQKVGQLTYEATRQHIKTLVGVDEGLISETLIDLYSK
                     QGIVAEPAGAASIASLEVLAEYIKGKTICCIISGGNNDINRMPEMEERALIYDGIKHY
                     FVVNFPQRPGALREFVNDILGPNDDITRFEYIKRASKGTGPVLIGIALADKHDYAGLI
                     RRMEGFDPAYINLNGNETLYNMLV"
     gene            complement(2129..2485)
                     /locus_tag="VYN69_000317"
     CDS             complement(2129..2485)
                     /locus_tag="VYN69_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001027901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1532668.1"
                     /translation="MNITNLFSIKTGCDETDRQLQKLFFQLDLQLGELTDQLRKLDSN
                     FVPRSQFVDTLDLNDVEYKEILNYFIFHRNDSEESLVEWLYDWISTNRYELPKEFSIR
                     MAHKYHESVTEVFGDE"
     gene            complement(2723..2959)
                     /locus_tag="VYN69_000318"
     CDS             complement(2723..2959)
                     /locus_tag="VYN69_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532669.1"
                     /translation="MLADQNKADWSYDENTVINIYDDANFEDGRLHMTFEQFFKLAQI
                     AREEGLEIHSSFERAGATKSARYIAKWILRNKKH"
     gene            complement(3066..4088)
                     /gene="ilvC"
                     /locus_tag="VYN69_000319"
     CDS             complement(3066..4088)
                     /gene="ilvC"
                     /locus_tag="VYN69_000319"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HEV1532670.1"
                     /translation="MTVQMEYEKDVKVAALDGKKIAVIGYGSQGHAHAQNLRDSGRDV
                     IIGVRPGKSFDKAKEDGFDTYTVTEATKLADVIMILAPDEIQQELYEAEIAPNLEAGN
                     AVGFAHGFNIHFEFIKVPADVDVFMCAPKGPGHLVRRTYEEGFGVPALYAVYQDATGN
                     AKNIAMDWCKGVGAARVGLLETTYKEETEEDLFGEQAVLCGGLTALIEAGFEVLTEAG
                     YAPELAYFEVLHEMKLIVDLIYEGGFKKMRQSISNTAEYGDYVSGPRVITEQVKENMK
                     AVLADIQNGKFANDFVNDYKAGRPKLTAYREQAANLEIEKVGAELRKAMPFVGKNDDD
                     AFKIYN"
     gene            complement(4154..4630)
                     /gene="ilvN"
                     /locus_tag="VYN69_000320"
     CDS             complement(4154..4630)
                     /gene="ilvN"
                     /locus_tag="VYN69_000320"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002927678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HEV1532671.1"
                     /translation="MRRMLTAKLQNRSGVLNRFTGVLSRRQVNIESISVGATEDPNVS
                     RITIIIDVASHDEVEQIIKQLNRQIDVIRIRDITDKPHLEREVILVKMSAPAEKRAEI
                     LAIIQPFRATVVDVAPSSITIQMTGNAEKSEALLRVIRPYGIRNIARTGATGFTRD"
     gene            complement(4623..6323)
                     /locus_tag="VYN69_000321"
     CDS             complement(4623..6323)
                     /locus_tag="VYN69_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HEV1532672.1"
                     /translation="MEKISLESPKTGSDLVLETLRDLGVDTIFGYPGGAVLPFYDAIY
                     NFKGIRHILGRHEQGCLHEAEGYAKSTGKLGVAVVTSGPGATNAITGIADAMSDSVPL
                     LVFTGQVARAGIGKDAFQEADIVGITMPITKYNYQVRETADIPRIITEAVHIATTGRP
                     GPVVIDLPKDISALETDFIYSPEVNLPSYQPTLEPNDMQIKKILKQLSKAKKPVLLAG
                     GGISYAEAATELNEFAERYQIPVVTSLLGQGTIATSHPLFLGMGGMHGSFAANIAMTE
                     ADFMISIGSRFDDRLTGNPKTFAKNAKVAHIDIDPAEIGKIISADIPVVGDAKKALQM
                     LLAEPTVHNNTEKWIEKVTKDKNRVRSYDKKERVVQPQAVIERIGELTNGDAIVVTDV
                     GQHQMWTAQYYPYQNERQLVTSGGLGTMGFGIPAAIGAKIANPDKEVVLFVGDGGFQM
                     TNQELAILNIYKVPIKVVMLNNHSLGMVRQWQESFYEGRTSESVFDTLPDFQLMAQAY
                     GIKNYKFDNPETLAQDLEVITEDVPMLIEVDISRKEQVLPMVPAGKSNHEMLGVQFHA
                     "
     gene            complement(6524..8191)
                     /locus_tag="VYN69_000322"
     CDS             complement(6524..8191)
                     /locus_tag="VYN69_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEV1532673.1"
                     /translation="MSKITTSLFQEMVQAASTRLNKQAEYVNSLNVFPVPDGDTGTNM
                     GMTIENGAKEVADKPASTVGEVASILAKGLLMGARGNSGVITSQLFRGFSQAIKDKDE
                     LTGQDLALAFQSGVEVAYKAVMKPVEGTILTVSRGAAIGAKKKAEQTDDTVEVMRAAL
                     EGAKTALAKTPDMLPVLKEVGVVDSGGQGLVFIYEGFLSALTGEYIASEDFVATPANM
                     SEMINVEHHKSVAGHVATEDITFGYCTEIMVALKQGPTYAKDFDYDEFRNYLDELGDS
                     LLVVNDDEIVKVHVHTEDPGLVMQEGLKYGSLVKVKVDNMRNQHEAQVEKEATQVIKS
                     AEEKEYALIAVVAGKGLADIFCSQGVDYVIEGGQTMNPSTEDFIKAVEQVNARNIIFL
                     PNNKNIFMAAQSAAEVLEQPAVVVEARTLPQGMTSLLAFDPSKSIEENQERMTAALSD
                     VVSGSVTTAVRDTTIDGLEIHENDNLGMVDGKILVSNPDMHQTLTETLKHMLDEDSEI
                     VTFYVGEDGSEELANEIAQEIVEEFEDVEVEIHQGQQPVYPYLFSVE"
     gene            complement(8194..8559)
                     /locus_tag="VYN69_000323"
     CDS             complement(8194..8559)
                     /locus_tag="VYN69_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1532674.1"
                     /translation="MTVKINTKDGQIELTDEVIATVVGGAATEIFGVVGMASKNALKD
                     NFQALLGKENYSKGVVVKAAEDGSIAVDVYTVLSYGTKISEVSKNIQERVAFSLENQL
                     GITAQTVNVYIQNIKVVGE"
     gene            complement(8716..8904)
                     /locus_tag="VYN69_000324"
     CDS             complement(8716..8904)
                     /locus_tag="VYN69_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008536053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEV1532675.1"
                     /translation="MAKVCYFTGRKTVSGNNRSHAMNQTKRAVKPNLQKVTVLIDGKP
                     KKVWASARALKSGKVERV"
     gene            complement(9020..9196)
                     /locus_tag="VYN69_000325"
     CDS             complement(9020..9196)
                     /locus_tag="VYN69_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001858191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1532676.1"
                     /translation="MDSDIVVRKSIDELWDLDLTAIPLAAVRDDFYTHNFNSGVLLIN
                     DGMWRAENVTQDLI"
     gene            complement(9307..9504)
                     /locus_tag="VYN69_000326"
     CDS             complement(9307..9504)
                     /locus_tag="VYN69_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 8"
                     /protein_id="HEV1532677.1"
                     /translation="MKEEKKAIVLGADNAYMDKVETTIKSLCVHHYNLKFYVFNDDLP
                     REWFQLMEKRLETLNSEIVNV"
     gene            complement(9759..11303)
                     /locus_tag="VYN69_000327"
     CDS             complement(9759..11303)
                     /locus_tag="VYN69_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEV1532678.1"
                     /translation="MNIQEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEICEAGTVK
                     GKKTGTFAKSDWMDIEKQRGISVTSSVMQFDYDGKRVNILDTPGHEDFSEDTYRTLMA
                     VDAAVMVVDSAKGIEAQTKKLFEVVKHRGIPVFTFMNKLDRDGREPLDLLQELEEILG
                     IASYPMNWPIGMGKAFEGLYDLYNQRLELYKGDERFASLEDGDKLFGSNPFYEQVKDD
                     IELLNEAGNEFSEEAILAGELTPVFFGSALTNFGVQTFLEIFLKFAPEPHGHKKTDGE
                     IVDPYDKDFSGFVFKIQANMDPRHRDRIAFVRIVSGEFERGMSVNLPRTGKGAKLSNV
                     TQFMAESRENVTNAVAGDIIGVYDTGTYQVGDTLTVGKNKFEFEPLPTFTPEIFMKVS
                     AKNVMKQKSFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAE
                     VVMSPMGKKTVRWIKPEDLDERMSSSRNILAKDRFDQPVFLFENDFALRWFADKYPDV
                     ELEEKM"
     gene            11516..11818
                     /gene="gatC"
                     /locus_tag="VYN69_000328"
     CDS             11516..11818
                     /gene="gatC"
                     /locus_tag="VYN69_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006147946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEV1532679.1"
                     /translation="MKITQEEVTHVANLSKLRFSEEETAAFATTLSKIVDMVELLGEV
                     DTTGVAPTTTMADRKTVLRPDVAEEGTDRDRLFKNVPEKDNYYIKVPAILDDGGDA"
     gene            11818..13284
                     /gene="gatA"
                     /locus_tag="VYN69_000329"
     CDS             11818..13284
                     /gene="gatA"
                     /locus_tag="VYN69_000329"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEV1532680.1"
                     /translation="MTFNNKTIEELHNLLVSKEISATELTQATLEDIKSREEALNSFV
                     TIAEEQALVQAKAIDEAGIDADNVLSGIPLAVKDNISTDGILTTAASKMLYNYEPIFD
                     ATAVANAKTKGMIVVGKTNMDEFAMGGSGETSHYGATKNAWDHSKVPGGSSSGSAAAV
                     ASGQVRLSLGSDTGGSIRQPAAFNGIVGLKPTYGTVSRFGLIAFGSSLDQIGPFAPTV
                     KENALLLNAIASEDAKDSTSAPVRIADFTSKIGQDIKGMKIALPKEYLGEGINPEVKE
                     TILNAAKHFEKLGAIVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRAEDA
                     TNLDEIYVNSRSQGFGEEVKRRIMLGTFSLSSGYYDAYYKKAGQVRTLIIQDFEKVFA
                     DYDLILGPTAPSVAYDLDSLNHDPVAMYLADLLTIPVNLAGLPGISIPAGFSQGLPVG
                     LQLIGPKYSEETIYQAAAAFEATTDYHKQQPVIFGGDN"
     gene            13284..14726
                     /gene="gatB"
                     /locus_tag="VYN69_000330"
     CDS             13284..14726
                     /gene="gatB"
                     /locus_tag="VYN69_000330"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEV1532681.1"
                     /translation="MNFETVIGLEVHVELNTNSKIFSPTSAHFGNDQNANTNVIDWSF
                     PGVLPVLNKGVVDAGIKAALALNMDLHKKMHFDRKNYFYPDNPKAYQISQFDEPIGYN
                     GWIEVELEDGTTKKIGIERAHLEEDAGKNTHGTDGYSYVDLNRQGVPLIEIVSEADMR
                     SPEEAYAYLTALKEVIQYAGISDVKMEEGSMRVDANISLRPYGQEKFGTKTELKNLNS
                     FSNVRKGLEYEVQRQAEILRSGGQIRQETRRYDEANKTTILMRVKEGAADYRYFPEPD
                     LPLFEISDEWIEEMRTELPEFPKERRARYVSDLGLSDYDASQLTANKVTSDFFEKAVA
                     LDGDAKQVSNWLQGEVAQFLNAEGKTLEQIELTPENLVEMIAIIEDGTISSKIAKKVF
                     VHLAKNGGGAREYVEKAGMVQISDPAILIPIIHQVFADNEAAVADFKSGKRNADKAFT
                     GFLMKATKGQANPQVALKLLAQELAKLKEN"
     gene            14855..15415
                     /gene="efp"
                     /locus_tag="VYN69_000331"
     CDS             14855..15415
                     /gene="efp"
                     /locus_tag="VYN69_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEV1532682.1"
                     /translation="MIEASKLKAGMTFETADGKLIRVLEASHHKPGKGNTIMRMKLRD
                     VRTGSTFDTSYRPEEKFEQAIIETVPAQYLYKMDDTAYFMNTETYDQYEIPVVNVENE
                     LLYILENSDVKIQFYGTEVIGVTVPTTVELTVAETQPSIKGATVTGSGKPATMETGLV
                     VNVPDFIEAGQKLVINTAEGTYVSRA"
     gene            15437..15826
                     /locus_tag="VYN69_000332"
     CDS             15437..15826
                     /locus_tag="VYN69_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1532683.1"
                     /translation="MGIEEQLGEIVIAPRVLEKIIAIATAKVEGVHSFSNRSVSDTLS
                     KLSLGRGIYLKNVDEELTADIYLYLEYGVKVPKVAVAIQKAVKDAVRNMADVELAAIN
                     IHVAGIVPDKTPKPELKDLFDEDFLND"
     gene            15819..16241
                     /gene="nusB"
                     /locus_tag="VYN69_000333"
     CDS             15819..16241
                     /gene="nusB"
                     /locus_tag="VYN69_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000203663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEV1532684.1"
                     /translation="MTSPLLESRRQLRKCAFQALMSLEFGTDVETACRFAYTHDREDT
                     DVQLPAFLIDLVSGVQAKKEELDKQITQHLKAGWTIERLTLVERNLLRLGVFEITSFD
                     TPQLVAVNEAIELAKDFSDQKSARFINGLLSQFVTEEQ"
     gene            complement(16290..16666)
                     /locus_tag="VYN69_000334"
                     /pseudo
     CDS             complement(16290..16666)
                     /locus_tag="VYN69_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244505.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
     gene            complement(16724..17010)
                     /locus_tag="VYN69_000335"
                     /pseudo
     CDS             complement(16724..17010)
                     /locus_tag="VYN69_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844738.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17028..17213)
                     /gene="briC"
                     /locus_tag="VYN69_000336"
     CDS             complement(17028..17213)
                     /gene="briC"
                     /locus_tag="VYN69_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm-regulating peptide BriC"
                     /protein_id="HEV1532685.1"
                     /translation="MTGTETFTVISTEDLEQTSGGLAVWEDGYSRWLYYREFAPYMRQ
                     GALNSYIDAWKYGFRTG"
     gene            complement(17476..18243)
                     /locus_tag="VYN69_000337"
     CDS             complement(17476..18243)
                     /locus_tag="VYN69_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEV1532686.1"
                     /translation="MNIAKIVREAREQSRLTTLDFATGIFDEFIQLHGDRSFRDDGAV
                     VGGIGWLGDQAVTVVGIQKGKSLQDNLKRNFGQPHPEGYRKALRLMKQAEKFGRPVVT
                     FINTAGAYPGVGAEERGQGEAIARNLMEMSDLKVPIIAIIIGEGGSGGALALAVADRV
                     WMLENSIYAILSPEGFASILWKDGTRAMEAAELMKITSHELLEMDVVDKVISEVGLSS
                     KELIKSVKKELQTELARLSQKPLEELLEERYQRFRKY"
     gene            complement(18240..19106)
                     /locus_tag="VYN69_000338"
     CDS             complement(18240..19106)
                     /locus_tag="VYN69_000338"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEV1532687.1"
                     /translation="MALFSKKDKYIRINPNRSVREKPQAKPEVPDELFSQCPGCKHTI
                     YQKDLGSERICPHCSYTFRISAQERLALTIDMGTFKELFTGIESKDPLHFPGYQKKLA
                     SMREKTGLHEAVVTGTALIKGQTVALGIMDSNFIMASMGTVVGEKITRLFEYATVEKL
                     PVVLFTASGGARMQEGIMSLMQMAKISAAVKRHSNAGLFYLTILTDPTTGGVTASFAM
                     EGDIILAEPQSLVGFAGRRVIENTVRESLPEDFQKAEFLLEHGFVDAIVKRRDLPDTI
                     ASLVRLHGGSPR"
     gene            complement(19143..20510)
                     /gene="accC"
                     /locus_tag="VYN69_000339"
     CDS             complement(19143..20510)
                     /gene="accC"
                     /locus_tag="VYN69_000339"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000488661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEV1532688.1"
                     /translation="MFRKILIANRGEIAVRIIRAARELGIATVAVYSTADKEALHTLL
                     ADEAVCIGPGKATESYLNINAVLSAAVLTEAEAIHPGFGFLSENSKFATMCEEVGIKF
                     IGPSGHVMDMMGDKINARAQMIKAGVPVIPGSDGEVHNSEEALIVAEKIGYPVMLKAS
                     AGGGGKGIRKVEKPDDLVSAFETASSEAKANYGNGAMYIERVIYPARHIEVQILGDEH
                     GHVIHLGERDCSLQRNNQKVLEESPSIAIGKTLRHEIGAAAVRAAEFVGYENAGTIEF
                     LLDEASSNFYFMEMNTRVQVEHPVTEFVSGVDIVKEQICIAAGQPLSVKQEDIVLRGH
                     AIECRINAENPAFNFAPSPGKITNLYLPSGGVGLRVDSAVYPGYTIPPYYDSMIAKII
                     VHGENRFDALMKMQRALYELEIEGVQTNADFQLDLISDRNVIAGDYDTSFLMETFLPK
                     YQEKE"
     gene            complement(20522..20944)
                     /gene="fabZ"
                     /locus_tag="VYN69_000340"
     CDS             complement(20522..20944)
                     /gene="fabZ"
                     /locus_tag="VYN69_000340"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEV1532689.1"
                     /translation="MIDIQGIKEALPHRYPMLLVDRVLEVSEDTIVAIKNVTINEPFF
                     NGHFPQYPVMPGVLIMEALAQTAGVLELSKPENKGKLVFYAGMDKVKFKKQVVPGDQL
                     VMTATFVKRRGTIAVVEAKAEVDGKLAASGTLTFAIGN"
     gene            complement(20941..21426)
                     /locus_tag="VYN69_000341"
     CDS             complement(20941..21426)
                     /locus_tag="VYN69_000341"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEV1532690.1"
                     /translation="MNLNDIKDLMTQFDQSSLREFSYKNGTDELQFSKNEARPVPEVA
                     TQVAPAPVLAPPSPVAPTSAPAETVAEEVPAPAEASVASEGNLVESPLVGVVYLAAGP
                     DKPAFVTVGDSVKKGQTLVIIEAMKVMNEIPAPKDGVVTEILVSNEEMVEFGKGLVRI
                     K"
     gene            complement(21429..22664)
                     /gene="fabF"
                     /locus_tag="VYN69_000342"
     CDS             complement(21429..22664)
                     /gene="fabF"
                     /locus_tag="VYN69_000342"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEV1532691.1"
                     /translation="MKLNRVVVTGYGVTSPIGNTPEEFWNSLATGKIGIGGITKFDHS
                     DFDVHNAAEIQDFPFDKYFVKKDTNRFDNYSLYALYAAQEAVNHANLDVEALNRDRFG
                     VIVASGIGGIKEIEDQVLRLHEKGPKRVKPMTLPKALPNMASGNVAMRFGANGVCKSI
                     NTACSSSNDAIGDAFRSIKFGFQDVMLVGGTEASITPFAIAGFQALTALSTTEDPTRA
                     SIPFDKDRNGFVMGEGSGMLVLESLEHAEKRGATILAEVVGYGNTCDAYHMTSPHPEG
                     QGAIKAIKLALEEAEISPEQVAYVNAHGTSTPANEKGESGAIVAVLGKEVPVSSTKSF
                     TGHLLGAAGAVEAIVTIEAMRHNFVPMTAGTSEVSDYIEANVVYGQGLEKEIPYAISN
                     TFGFGGHNAVLAFKRWENR"
     gene            complement(22686..23417)
                     /gene="fabG"
                     /locus_tag="VYN69_000343"
     CDS             complement(22686..23417)
                     /gene="fabG"
                     /locus_tag="VYN69_000343"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEV1532692.1"
                     /translation="MKLEHKNIFITGSSRGIGLAIAHKFAQAGANIVLNSRGAISEEL
                     LAEFSNYGIKVVPISGDVSDFADAKRMIDQAIAELGSVDVLVNNAGITQDTLMLKMTE
                     ADFEKVLKVNLTGAFNMTQSVLKPMMKAREGAIINMSSVVGLMGNIGQANYAASKAGL
                     IGFTKSVAREVASRNIRVNVIAPGMIESDMTAILSDKIKEATLAQIPMKEFGQAEQVA
                     DLTVFLAGQDYLTGQVVAIDGGLSM"
     gene            complement(23451..24371)
                     /gene="fabD"
                     /locus_tag="VYN69_000344"
     CDS             complement(23451..24371)
                     /gene="fabD"
                     /locus_tag="VYN69_000344"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEV1532693.1"
                     /translation="MTKTAFLFAGQGAQYLGMGRDFYDQYPIVKETIDRASQVLGYDL
                     RYLIDTEEDKLNQTRYTQPAILATSVAIYRLLQEKGYQPDMVAGLSLGEYSALVASGA
                     LDFEDAVALVAKRGAYMEEAAPADSGKMVAVLNTPVEVIEEACQKASELGVVTPANYN
                     TPAQIVIAGEVVAVDRAVELLQEAGAKRLIPLKVSGPFHTALLEPASQKLAETLAQVS
                     FSDFTCPLVGNTEAAVMQKEDIAQLLTRQVKEPVRFYESIGVMQEAGISNFIEIGPGK
                     VLSGFVKKIDQTAHLAHVEDQASLVALLEK"
     gene            complement(24364..25338)
                     /gene="fabK"
                     /locus_tag="VYN69_000345"
     CDS             complement(24364..25338)
                     /gene="fabK"
                     /locus_tag="VYN69_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-[acyl-carrier-protein] reductase FabK"
                     /protein_id="HEV1532694.1"
                     /translation="MKTRITELLKIDYPIFQGGMAWVADGDLAGAVSKAGGLGIIGGG
                     NAPKEVVKANIDKIKSLTDKPFGVNIMLLSPFVEDIVDLVIEEGVKVVTTGAGNPSKY
                     MERFHEAGIIVIPVVPSVALAKRMEKIGADAVIAEGMEAGGHIGKLTTMTLVRQVATA
                     VSIPVIAAGGIADGEGAAAGFMLGAEAVQVGTRFVVAKESNAHPNYKEKILKARDIDT
                     TISAQHFGHAVRAIKNQLTRDFELAEKDAFKQEDPDLEIFEQMGAGALAKAVVHGDVE
                     GGSVMAGQIAGLVSKEETAEEILKDLYYGAAKKIQEEASRWTGVVRND"
     gene            complement(25457..25681)
                     /locus_tag="VYN69_000346"
     CDS             complement(25457..25681)
                     /locus_tag="VYN69_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1532695.1"
                     /translation="MAVFEKVQEIIVEELGKDASEVTLESTFDDLDADSLDLFQVISE
                     IEDAFDIQIEAENDLKTVGDLVAYVEEQAK"
     gene            complement(25741..26715)
                     /locus_tag="VYN69_000347"
     CDS             complement(25741..26715)
                     /locus_tag="VYN69_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEV1532696.1"
                     /translation="MAFAKISQVAHYVPEQVVTNHDLAQIMDTNDEWISSRTGIRQRH
                     ISRTESTSDLATEVAKKLMAKAGITGEELDFIILATITPDSMMPSTAARVQANIGANK
                     AFAFDLTAACSGFVFALSTAEKFIASGRFQKGLVIGSETLSKAVDWSDRSTAVLFGDG
                     AGGVLLEASEQEHFLAESLNSDGSRSECLTYGHSGLHSPFSDQESADSFLKMDGRTVF
                     DFAIRDVAKSIKQTIDESPIEVTDLDYLLLHQANDRILDKMARKIGVDRAKLPANMME
                     YGNTSAASIPILLSECVEQGLIPLDGSQTVLLSGFGGGLTWGTLILTI"
     gene            complement(26715..27149)
                     /locus_tag="VYN69_000348"
     CDS             complement(26715..27149)
                     /locus_tag="VYN69_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1532697.1"
                     /translation="MDYQRINEYLTSIFNNVLVIEEVNLRGSRFKDISIKEMHTIDVI
                     GKAPDVTPSQVSKELMVTLGTVTTSLNNLERKGYIERVRSEQDRRVVHLHLTKKGRLI
                     HRLHKRFHKAMVEKIIDGMSEEEIAVMGKGLTNLYQFLEDLK"
     gene            complement(27243..28028)
                     /locus_tag="VYN69_000349"
     CDS             complement(27243..28028)
                     /locus_tag="VYN69_000349"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HEV1532698.1"
                     /translation="MEHIIYQLEEDLAILTLNRPEVANGFHIPMCEEILEALTLAEEN
                     PAVHFILINANGKVFSVGGDLVEMKRAVDEDDIPSLTKIAELVNTISYKIKQIAKPVL
                     MEVDGAVAGAAANMAVAADFCLATDKAKFIQAFVGVGLAPDAGGIHLLSRSIGVTRAA
                     QLAMTGEALTAEKALEWGLVYRVSEAEKLEKTREQLLKKLRRASSNSYAAIKKLVWES
                     QFKDWQGYATLELNLQKSLAQTEDFKEGVRAHSERRRPKFTGK"
     gene            28397..29761
                     /locus_tag="VYN69_000350"
     CDS             28397..29761
                     /locus_tag="VYN69_000350"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEV1532699.1"
                     /translation="MKVVKFGGSSLASASQLEKVLNIVKSDSERRFVVVSAPGKRNAE
                     DTKVTDALIKYYRDYVAGNDISKSQSWIIDRYAAMVSELGLKPAVLEKISKSIHALAT
                     LPIEENEFLYDTFLAAGENNNAKLIAAYFNQNGIDARYMHPREAGIVVTSEPGHARII
                     PSSYDKIEELTNTNEVLVIPGFFGVTKENQSCTFSRGGSDITGSIIAAGVKADLYENF
                     TDVDGIFAAHPGIIHQPHSIPELTYREMRELAYAGFSVLHDEALLPAYRGKIPLVIKN
                     TNNPDHPGTRIVLKHSNDEFPVVGIAGDSGFVSINMSKYLMNREVGFGRKVLQILEEL
                     NIGWEHMPTGIDDLSIILRSRQLTPIKEEEILRQLVQKAKVDHAEIEHDLSIIMIVGE
                     KMKSHIGVTATATRALSENKINIQMMSQGSSEVSIMFVVNKDQEKAAIKALYNAFFGE
                     SKED"
     gene            29764..30135
                     /locus_tag="VYN69_000351"
     CDS             29764..30135
                     /locus_tag="VYN69_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEV1532700.1"
                     /translation="MAQSLNKTVLLSTTGTSYLSIAGKVGKFLVGDQALEFYPDVNVE
                     QFIQIPWSHINQIGANVTGRKISRHFEVFTDKGKFLFASKDSGAILKIAREKLGNDKV
                     VKLPTLIQTISQKFKNLFAKK"
     gene            30349..31623
                     /gene="serS"
                     /locus_tag="VYN69_000352"
     CDS             30349..31623
                     /gene="serS"
                     /locus_tag="VYN69_000352"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEV1532701.1"
                     /translation="MLDIKRIRTDFEAVAEKLATRGVDAAVLNEMKEIDAKRRNILVK
                     VETLKAERNTVSAEIAQAKRNKENTDDKIAAMQNLSAEVKALDAELAEIDAKLTEFTT
                     TLPNIPADSVPVGADEDDNVEVRRWGTPREFDFEPKAHWDLGEDLGILDWERGGKVTG
                     ARFLFYKGLGARLERAIYNFMLDEHGKEGYTEVITPYIVNHDSMFGTGQYPKFKEDTF
                     ELSDTNFVLIPTAEVPLTNYYRDEILDGKDLPIYFTAMSPSFRSEAGSAGRDTRGLIR
                     LHQFHKVEMVKFAKPEESYEELEKMTANAENILQKLNLPYRVVALSTGDMGFSATKTY
                     DLEVWIPAQNNYREISSCSNTEDFQARRAQIRYRDEADGKVKLLHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTIPEALRPYMGGAEVIKP"
     gene            complement(31648..32547)
                     /locus_tag="VYN69_000353"
     CDS             complement(31648..32547)
                     /locus_tag="VYN69_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000735961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEV1532702.1"
                     /translation="MKKLPLVFSGCLLGLAGAGNLILDTLPVLSHLFSLTGLVLWIYF
                     LILHLFNWKETKQELTKPPLLSGMATFPMAGMILSTYVFRVFSYLPLVAQGIWWFSFL
                     LDLTLIAGFTIKFACPGRRVHATPSWTVLYVGIAVAALTYPLVGIIEIAYATLSFGFL
                     LTFYLYPLIYSDLKKHPLPLALLGQEGIYCAPFSLLLASLVRVGGTSLPTWVLIVMIL
                     ASQSFFFFVLTRLPNILKQGFQPAFSALTFPTIITATSLKMAQGILKLPFLDYLVLTE
                     TIICLTILFFVLGAYLIWLRKKV"
     gene            complement(32657..33205)
                     /locus_tag="VYN69_000354"
     CDS             complement(32657..33205)
                     /locus_tag="VYN69_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000206764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEV1532703.1"
                     /translation="MTTFTIHTVESAPAEVKEILETVEKDNNGYIPNLIGLLANAPTV
                     LEAYQIVSSIHRRNSLTPVEREVVQITAAVTNGCAFCVAGHTAFSIKQIQMNDDLIQA
                     LRNRTPIETDPKLDTLAKFTLAVINTKGRVGDEALSEFLEAGYTQQNALDVVFGVSLA
                     ILCNYANNLANTPINPELQPYA"
     gene            complement(33423..34745)
                     /locus_tag="VYN69_000355"
     CDS             complement(33423..34745)
                     /locus_tag="VYN69_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
                     /protein_id="HEV1532704.1"
                     /translation="MLELLKSIDAFAWGPPLLILLVGTGIYLTMRLGLLQVLRLPKAF
                     QLIFIQDKGHGDVSSFAALCTALASTVGTGNIIGVATAIKVGGPGALFWMWMAAFFGM
                     ATKYAEGLLAIKYRTKDDHGAVAGGPMHYILLGMGEKWRPLAVLFAVAGVLVALLGIG
                     TFTQVNSITESIQNTTTISPAITALVLSVFVAIAVFGGLKSISKVSTTVVPFMALIYI
                     LGTLTVIFFNIGKIPGTIALVFTSAFSPLAAVGGFAGASVRMAIQNGVARGVFSNESG
                     LGSAPIAAAAAKTNEPVEQGLISMTGTFIDTLIICTLTGLTILITGVWSGDLNGVALT
                     QSAFSTVFSHFGPALLTIFLVLFAFTTILGWNYYGERCFEFLFGARFIWLYRVVFVLM
                     VLLGGFIELDMVWIIADIVNALMALPNLIALLVLSPVVIAETKKYFDK"
     regulatory      complement(34836..34924)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            complement(35087..37423)
                     /locus_tag="VYN69_000356"
     CDS             complement(35087..37423)
                     /locus_tag="VYN69_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEV1532705.1"
                     /translation="MNKKILETLEFDKVKALFEPHLLTEQGLEQLRQLAPTAKADKIK
                     QAFAEMKEMQALFVEQPHFTILSTKEIAGVCKRLEMGADLNIEEFLLLKRVLLASREL
                     QSFYANLENVSLEELALWFEKLHDFPQLQGNLQAFNDAGFIENFASEELARIRRKIHD
                     SESQVRDVLQDLLKQKAQMLTEGIVASRNGRQVLPVKNTYRNKIAGVVHDISASGNTV
                     YIEPREVVKLSEEIASLRADERYEMLRILQEISERVRPHAAEIANDAWIIGHLDLIRA
                     KVRFIQERQAVVPQLSENQEIQLLHVCHPLVKNAVANDVHFGQDLTAIVITGPNTGGK
                     TIMLKTLGLTQVMAQSGLPILADKGSRVGIFEEIFADIGDEQSIEQSLSTFSSHMTNI
                     VDILGKVNQHSLLLLDELGAGTDPQEGAALAMAILEDLRLRQIKTMATTHYPELKAYG
                     IETAFVQNASMEFDTATLRPTYRFMQGVPGRSNAFEIAKRLGLSEVIVGDASQQIDQD
                     NDVNRIIEQLEEQTLESRKRLDNIREVEQENLKMNRALKKLYNELNREKETELNKARE
                     QAAEIVDMALSESDQILKNLHSKSQLKPHEIIEAKAKLKKLAPEKVDLSKNKVLQKAK
                     KKRAPKVGDDIVVLSYGQRGTLTSQLKDGRWEAQVGLIKMTLEEKEFDLVQAQQEKPV
                     KKKQVNVVKRTSGRGPQARLDLRGKRYEEAMNELDTFIDQALLNNMAQVDIIHGIGTG
                     VIREGVTKYLQRNKHVKSFGYAPQNAGGSGATIVTFKG"
     gene            complement(37527..38075)
                     /locus_tag="VYN69_000357"
     CDS             complement(37527..38075)
                     /locus_tag="VYN69_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEV1532706.1"
                     /translation="MISFLLLLVLVWGFYIGYRRGLLLQVYYLISAMASAFMAGQFYK
                     GLGEQFHLLLPYANSQEGQGTFFFPSDQLFQLDKVFYAGIGYLLVFGIVYSIGRLLGL
                     LLHLIPSKKLGGKLFQVSAGILSMLVTLFVLQMALTILATIPMAVIQNPLEKSIVAKH
                     IIQSIPVTTSWLKQIWVTNLIG"
     gene            complement(38072..38374)
                     /gene="zapA"
                     /locus_tag="VYN69_000358"
     CDS             complement(38072..38374)
                     /gene="zapA"
                     /locus_tag="VYN69_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEV1532707.1"
                     /translation="MANLNRFKFTFGKKSLTLTSEHDNLFMEEIAKVATEKYQAIKEQ
                     MPSADDETIALLLAVNCLSTQLSREIEFDDKEQELEELRHKLVTCKQEQSKIEDSL"
     gene            38461..39342
                     /locus_tag="VYN69_000359"
     CDS             38461..39342
                     /locus_tag="VYN69_000359"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEV1532708.1"
                     /translation="MASITLTPSEKDIQAFLEHYQTSLAPSKNPYIRYFLKLPQATVS
                     IYTSGKILLQGEGAEKYASFFGYQAVEQTSGQNLPLIGTDEVGNGSYFGGLAVVAAFV
                     TSDQHDFLRKLGVGDSKTLTDQKIRQIAPILKEKIQHQALLLSPSKYNEVIGDRYNAV
                     SVKVALHNQAIYLLLQKGVQPEKIVIDAFTSAKNYDKYLAQEANRFSNPISLEEKAEG
                     KYLSVAVSSVIARDLFLENLENLGRELGYQLPSGAGTASDKVASQILQAYGMQGLSFC
                     AKLHFKNTEKAKKRLER"
     gene            39346..39960
                     /gene="lepB"
                     /locus_tag="VYN69_000360"
     CDS             39346..39960
                     /gene="lepB"
                     /locus_tag="VYN69_000360"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEV1532709.1"
                     /translation="MNSFKNFLKEWGLFLLILSLLALSRIFFWSNVRVEGHSMDPTLA
                     DGEILFVVKHLPIDRFDIVVAHEEDGNKDIVKRVIGMPGDTIRYENDKLYINDKETDE
                     PYLADYIKRFKDDKLQSTYSGKGFEGNKGTFFRSIAQKAQAFTVDVNYNTNFSFTVPE
                     GEYLLLGDDRLVSSDSRHVGTFKAKDITGEAKFRFWPITRIGTF"
     gene            40088..42454
                     /locus_tag="VYN69_000361"
     CDS             40088..42454
                     /locus_tag="VYN69_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEV1532710.1"
                     /translation="MEVYFSGTIERIIFENPSNFYRILLLEIDDTDAEDFDDFEIIVT
                     GTMADVIEGEDYTFWGQIVQHSKYGEQLQISRYDRAKPTSKGLVKYFSSSHFKGIGLK
                     TAQKIVDTYGENTIDEILQHPEKLEGITGLSAKNREAFVSTLRLNYGTEMVLAKLANY
                     GIPNKLAFQIQDFYKEETLDVVENYPYQLVEDIKGLGFTIADQLAEELGIESQAPERF
                     RAGLVHSLFQACMETGDTYVEARDLLEQTLTLLESSRPVELDPSQVAQELSYLIEEDK
                     VQQIDTKIFDNSLFFAEEGIRSHLVRILEKGKQKSHDLETIQKHITTIEEELGIEYDN
                     IQKQAICDAIQNKVFILTGGPGTGKTTVINGIIAVYALLEGLDFRKKSNLPILLAAPT
                     GRAARRMNELTGLPSATIHRHLGMTGDDDTSHLEDYLDAEFIIVDEFSMVDTWLANQL
                     FSNISSNSKILIVGDSDQLPSVSPGQVLADLLHIPLIPQTRLEKIYRQSKESTIVTLA
                     SQIRQGILPADFTQKKADRSYFEIASGHIPATIEKILGAALRNGIPARDIQVLAPMYR
                     GTAGIDAINQLMQDLLNPPQKDQLSFEAPQCHYRKRDKVIHLVNDAEINVFNGDLGAI
                     TDLIPGKYTESKQDEIVIDFDGNEVSYPRNEWYKIRLAYAMSIHKSQGSEFPVVILPI
                     TSASRRMLERNLIYTAITRAKSKLILLGELQAFDYATQHIGTARKTYLIERFSDLLEN
                     VEEKQQAVSETVTSSASEQSYILTEENWDRIPAMIGITDTDLKEIFGK"
     gene            complement(42502..43785)
                     /locus_tag="VYN69_000362"
     CDS             complement(42502..43785)
                     /locus_tag="VYN69_000362"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEV1532711.1"
                     /translation="MSVSFENKETNRGVLTFTISQDQIKPELDRVFKSVKKSLNVPGF
                     RKGHLPRPIFDKKFGEESLYQDVMNALLPNAYEAAVKEAGLEVVAQPKIDVTSMEKGQ
                     DWVIAAEVVTKPEVKLGDYKNLEVSVDVEKEVTDADVEERIERERNNLAELVIKEAAA
                     ENGDTVVIDFVGSIDGVEFDGGKGENFSLGLGSGQFIPGFEDQLVGHSAGETVDVIVT
                     FPEDYQAEDLAGKEAKFVTTIHEVKAKEVPALDDELAKDIDEEVETLADLKEKYRKEL
                     AAAKEEAYKDAVEGAAIDTAVENAEIVELPEEMIHEEVHRSVNEFLGNLQRQGINPDM
                     YFQITGTTQEDLHNQYQAEAESRTKTNLVIEAVAKAEGFDASEEEIQKEVEQLAADYN
                     MEVAQVQNLLSADMLKHDITIKKAVELITSTATVK"
BASE COUNT        12775 a        10081 c         8310 g        12722 t
ORIGIN      
        1 ttgtcaataa aaaaacatgt attcaacgaa aataattagt tcagaatttt agcttcagaa
       61 ttttcttact tcctttttga atccgataat atataggttt tactagtctc tacaccataa
      121 atatttgcta ataaaaatac tgtattatta ccctcttaag gtgcgaaatt attcaagttc
      181 ttaaaaaatg cttctctatt tccgtaagat agcttatttc ttcgatttaa gaactgaaga
      241 tgatagttct aacctttaga aatccaagcc ttcactagct caaaaactcc tatctttctt
      301 cctcaataaa tcatctgaga gataggtatt ccttatatcc ttcaaattca tcactccttt
      361 ggatattgag gtgctagcct agaagcagac acccgattat ctaaaccatc cttaatagag
      421 atgacaagac agataagctc agttaattac ttatatatct ttttaatatg gtatatttgt
      481 caacaaaaag aaatcaaatc ataccttata ctcaatgaaa atcaaagagc aaactaggaa
      541 gctagccgca agttgctcaa aacactgttt tgaggttgca gatagagcta acgtggtttg
      601 aagagatttt cgaagagtat taaagacagt gtgactatgc ttgtcaatag ataggaaatc
      661 aaaatgaagg atggaaatct gagccaaaca tcataagtgc tataatgaac agaagtaaga
      721 attgcacgat aagtttccta aaaccataga aatcgtcccc tttcttcatc ttcattatag
      781 cacatataca tcagttgtac aaataatatg atattttttt attagtcctc agacaagcat
      841 attataaagc gtttcattac catttaagtt aatataagct ggatcaaaac cttccattct
      901 acgaatcaaa cctgcataat catgcttatc tgctaaagcg atcccaatta atactgggcc
      961 tgttcccttg ctagctcgtt tgatatactc aaaacgtgtg atatcatcat ttggccccag
     1021 gatatcattt acaaactcac gcaaagctcc tggacgttgt gggaaattga ccacaaagta
     1081 atgtttgata ccatcataaa tcaaggcacg ctcttccatt tctggcatac ggttgatatc
     1141 attatttcct ccagaaatga tacaacaaat ggttttcccc ttaatatatt cagctaaaac
     1201 ctctaaagag gcgatactag ccgctccagc aggttctgcg actatccctt gcttagagta
     1261 aaggtcaatc aaggtttcag aaatcaatcc ctcatcgaca cctaccaaag ttttaatatg
     1321 ttgacgagtt gcttcatagg tcaactgacc taccttttgc acagcaatcc catccgcaaa
     1381 tttatcaatt tccttgagtt tgactggacc tccagcctca aaggcagctt tcatggaacg
     1441 cgctccattc gcctctactc cgataacctc aatctctgga cttgtttcct tgatataggt
     1501 agaaaccccg gcaatgagac cgccaccacc aacaggaacc aagacagcat caaaatcaat
     1561 cgattctttt cgagcttctt ctaaaatctc ataagcaact gttccttgac ctgcttgaac
     1621 atgagcatca tcaaaaggat caataaaggt acgattttca gagactgtaa attcttgagc
     1681 tgctttggct gaggcatcaa aggtatctcc aactagttta atagttacaa aatccccacc
     1741 aaaaaagcga acctgaccaa tcttttgttg tggcgtagta ataggcataa agatagtagc
     1801 aggaattttc atttcattac aagtataggc tactccctgc gcatgatttc ccgcagaagc
     1861 gcagactacc ccacgttcac gttcttcctt gctgagctgg gaaatggcat aataggcacc
     1921 acgaatttta aaggagcgaa cacgctgggc attttctttt ttcaaataaa tcttagcacc
     1981 atacttctcc gataaataat gatcgtaatc cagtggagta ttcacaacca caccgttcaa
     2041 gatcttgtga gccttgatta tatcttttga acttaacata taaaacctct ttttctataa
     2101 aaaacagtca ctaatgactg ttttttagtt attcatctcc gaaaacttca gtaacacttt
     2161 catggtattt atgagccata cgaatcgaaa actctttagg aagctcataa cgatttgtgg
     2221 aaatccaatc atataaccat tctaccaaac tttcttcact atcattacga tggaagataa
     2281 aatagtttaa aatttcttta tattctacat cattcaaatc caacgtgtct acaaattgac
     2341 tacgaggaac aaaattagaa tctaattttc ttagttgatc tgtcaattct cccaattgta
     2401 aatccaactg aaaaaatagt ttttgcagtt gcctatcagt ttcatcacat cctgtcttga
     2461 tagaaaacaa attggtaata ttcatatgat ttcaacccct aaataagtac atctaatatc
     2521 ccacgaatag ctgaagctaa agttttcgca gtccagtcat ttaaacccga ttctttcaat
     2581 ccagatgcaa taatttgctc tatttgatat aaaccgccat ctatcgtctc atttattctg
     2641 cgaagaggac cttcaatata ggataaagct tctccgaatc taacagctga tttaggtcct
     2701 aatgatttac caactatatt tgttaatgtt ttttatttct caaaatccat ttcgctatat
     2761 aacgagcaga tttagtcgca ccagctctct caaacgaaga atgaatttca agaccttctt
     2821 ctctagctat ttgtgccaat ttgaagaatt gttcaaaggt catatgcaac ctaccatctt
     2881 caaaattagc atcatcataa atgttaatta cagtattttc atcatacgac caatctgctt
     2941 tattttggtc tgctagcact attaccgacg gtgtaattac attatacaac tagaaccaat
     3001 aataacgatg caatttttct cataatcttt ttcataaaat catctccagc gctatatatt
     3061 tctaattagt tatagatttt gaatgcatca tcgtcgtttt taccaacgaa tggcattgct
     3121 ttacgcaatt ctgcaccaac tttttcaatt tcaaggttag ctgcttgttc acggtaagca
     3181 gtcaattttg gacgtccagc tttatagtca tttacaaagt catttgcaaa tttaccattt
     3241 tggatgtctg ccaagacagc cttcatattt tctttaactt gttcagtgat tacacgtgga
     3301 cctgatacat agtcaccgta ttcagcagtg tttgaaatag attgacgcat tttcttgaat
     3361 ccaccttcgt agatcaagtc aacgatcaat ttcatttcgt gaagaacttc aaagtaagcc
     3421 aattctggag cgtaacctgc ttctgtcaag acttcgaaac ctgcttcgat aagggcagtc
     3481 aaaccaccac aaagtacagc ttgttcacca aacaaatctt cttcagtttc ttctttgtaa
     3541 gttgtttcaa gaagacctac acgagccgct ccaacacctt tacaccagtc catagcaatg
     3601 tttttagcat ttcctgttgc atcttggtat actgcataaa gagctggaac accaaatcct
     3661 tcttcgtaag tacgacgtac caagtgtcct ggtcctttag gagcacacat gaagacatct
     3721 acatccgcag gaactttgat aaattcaaag tggatgttga aaccatgggc aaatccaact
     3781 gcgtttccag cttccaagtt tggagcgatt tctgcttcgt acaattcttg ttgaatttcg
     3841 tctggcgcca agatcatgat aacatcagcc aacttagtag cttctgttac tgtgtaagta
     3901 tcaaatccat cttcttttgc tttatcaaaa gatttacctg gacgtacacc gataataacg
     3961 tcacgacctg aatcacgcaa gttttgagca tgcgcatgcc cttgtgaacc ataaccgata
     4021 acggcgattt ttttaccgtc aagtgctgct actttaacat ctttttcata ttccatttga
     4081 actgtcatag cttttctctc ttttctatta tttattgcct tttaagctgg tttaacaaat
     4141 ttaagttgga tttttaatcg cgggtaaatc cagttgcacc cgttcgagca atattgcgaa
     4201 taccgtatgg gcgaatgact cgcaataggg cttcgctctt ttctgcattt cccgtcatct
     4261 gaatggtaat cgagcttggc gctacgtcta ccactgttgc acggaaaggt tgaataatcg
     4321 ctaaaatctc agctctcttc tcagctggcg ctgacatctt aaccaaaatc acctcgcgct
     4381 ccaaatgagg cttgtctgta atatctcgaa tgcgaatcac atcaatctga cgattgagct
     4441 gtttgatgat ttgctccact tcatcatgag aagcaacatc aataataata gtgatacgcg
     4501 atacattcgg atcttctgtt gctccaacag agatgctttc aatattaacc tgacgacgag
     4561 ataggacacc tgtaaagcga ttgaggactc ctgaacgatt ttgtagtttt gctgttaaca
     4621 ttctacgcat ggaactgcac ccccaacatc tcatgattac tcttaccagc cggtaccatt
     4681 ggtaacacct gttccttacg agaaatatct acctcaatta gcataggaac atcctcagtg
     4741 atgacttcaa ggtcttgagc caaggtctca ggattgtcaa acttatagtt tttaatacca
     4801 taagcctgcg ccatcaattg gaaatcagga agggtatcaa agaccgactc tgatgttctg
     4861 ccttcataga aggattcctg ccactggcga accattccga gtgaatgatt gttcagcata
     4921 accaccttga ttggcacctt gtaaatattc aaaatagcca actcctggtt ggtcatttgg
     4981 aaaccaccat ccccaacaaa caagactact tccttatctg ggttagcaat tttagcaccg
     5041 attgctgctg gaattccaaa gcccattgtt cccaaaccac ctgaagtcac taactgacgt
     5101 tcattttggt agggataata ctgagctgtc cacatttggt gttgaccaac gtctgttacc
     5161 acaatggcat ctccattcgt caattcacca attcgttcaa taactgcttg cggttgaacc
     5221 acacgctctt tcttgtcata agaacgaaca cgattcttgt ctttagtgac tttctcaatc
     5281 cacttttcag tattgttgtg aactgttggt tctgctagca acatttgcaa ggccttctta
     5341 gcatctccaa ctacaggaat gtctgcactg ataatcttgc caatctcagc tgggtcaata
     5401 tcaatgtggg caaccttagc attcttagcg aaagtcttag gattccccgt caaacggtca
     5461 tcgaaacgag aaccaatact aatcataaag tccgcttccg tcatggcaat atttgctgcg
     5521 aatgacccgt gcatgcctcc cattccaaga aagagtgggt gactcgttgc aatcgttcct
     5581 tgtcccaaaa gactggttac cactggaatt tgatagcgtt ctgcaaattc atttagttcc
     5641 gtagcagcct cagcataact aattccacca ccagctaaca agactggctt tttagccttg
     5701 gacaattgct tcaagatttt cttgatttgc atatcattcg gctcaagagt cggctgataa
     5761 cttggtaaat tcacttctgg tgaataaatg aagtctgttt ctaaagcaga tatgtctttt
     5821 ggtaggtcaa ttacaactgg ccctggacgg cctgtagttg cgatatggac agcttccgta
     5881 atgatacgcg gaatatcagc tgtctcacga acttggtaat tgtacttagt gattggcatg
     5941 gtaattccca cgatgtctgc ctcctgaaag gcatccttcc caatccctgc tcgcgccacc
     6001 tgacctgtaa agaccaaaag gggaacgcta tcgctcatag catccgcaat ccctgtaatg
     6061 gcatttgttg ctcctggtcc actagtgacg acggcaacac ccaactttcc agttgatttg
     6121 gcataacctt cagcttcatg caaacaacct tgctcatggc gccctagaat gtggcgaatg
     6181 cctttaaaat tatatatcgc atcataaaaa ggcaaaaccg caccaccagg ataaccaaag
     6241 atggtatcaa ctcctaaatc acgaagtgtt tccaaaacta ggtccgaccc cgtcttagga
     6301 gattctaaac tgattttctc cattgttccc ctttctttcc tcttaaaaat aacttgttac
     6361 tatcatacca ttttttcaaa atttttcaag ataaaagaag aaattttctg aattttctat
     6421 tttaacgttt atttatgaat gttatttttg tttttattaa aaagataccg atttcattta
     6481 ctaaaaaaga aaaaagaact gatttctcag tccttcatta atcttattcc acactaaata
     6541 ggtatgggta aacaggttgt tgaccttggt gaatctcgac ttcaacgtct tcgaattctt
     6601 ctacgatttc ttgagcgatt tcattagcaa gttcttcgct tccgtcttca ccgacataga
     6661 aggttacgat ttcactgtct tcatccaaca tatgtttcaa ggtttcagtc aatgtttggt
     6721 gcatatcagg gtttgacaca agaatttttc catccaccat acctagatta tcgttttcat
     6781 ggatttctaa gccatcgatc gttgtatcac gcacggctgt tgtgacgctt ccgctaacaa
     6841 catcgctaag agcagctgtc atacgctctt ggttttcttc aatggacttg cttggatcaa
     6901 aggcaagaag acttgtcata ccttgaggaa gagtgcgagc ctctaccact actgctggtt
     6961 gctccaaaac ttctgccgca gattgagctg ccatgaagat gttcttgttg tttggcaaga
     7021 agatgatgtt acgggcattg acctgttcaa cagccttgat aaagtcttct gttgaagggt
     7081 tcatggtttg accgccttcg ataacataat ccacgccttg agaacagaag atatctgcta
     7141 gacctttacc agccaccaca gcaatcaaag catactcttt ttcttcagcc gacttgataa
     7201 cttgagtagc ttctttctca acctgtgctt cgtgttggtt acgcatattg tcaactttta
     7261 ccttgaccaa gctaccatat ttgagacctt cttgcataac aagtcctgga tcttctgtat
     7321 gaacatggac tttgacaatt tcatcatcgt taacaacgag gagagaatcc ccaagctcat
     7381 ccaagtagtt acggaattca tcgtagtcaa aatctttagc ataggttgga ccttgcttaa
     7441 gagctaccat gatttcagta cagtaaccaa acgtgatgtc ctcagtcgct acgtgaccag
     7501 ctacagactt atgatgctct acattgatca tctcactcat gttggcagga gtcgctacaa
     7561 agtcctcaga tgcaatatat tcgccagtaa gggctgaaag gaaaccttcg tagatgaaga
     7621 ccaatccttg accacctgag tccacaacgc caacttcttt caatactgga agcatgtctg
     7681 gtgttttagc tagagctgtt ttagcacctt ccaaggctgc gcgcatgact tcaacagtat
     7741 catctgtttg ctcagctttt ttcttagcac cgatagcagc tccacgagaa actgtcaaaa
     7801 tcgttccttc aacaggtttc atcactgcct tataggcaac ttccacacct gattggaagg
     7861 ccagagccaa gtcttgacct gttaactcgt ctttatcctt gatagcttgg gaaaatccac
     7921 ggaaaagctg agatgtaatc actcctgagt tcccacgcgc acccatcaaa agccctttgg
     7981 caagaatgct cgctacttct ccaactgtag aagctggctt gtctgcaact tctttagcac
     8041 cattttcaat ggtcattccc atatttgtcc cagtatctcc atctggaact ggaaagacgt
     8101 ttaatgaatt gacatattca gcttgcttat tcaagcgagt tgatgcagcc tgcaccattt
     8161 cttgaaataa gctagtagta atctttgaca cggttattct cctacaactt tgatattttg
     8221 aatgtagaca tttacagtct gagcagtaat tccaagctgg ttttccaagc taaaggcaac
     8281 acgctcttga atgttttttg acacttcact aatctttgtt ccgtagctta acacggtata
     8341 tacatcaact gcaatactgc catcttcggc tgcctttacg acgacacctt tagaataatt
     8401 ttccttacct agcagggctt ggaaattatc tttgagggca tttttactag ccataccgac
     8461 cacaccaaaa atctcagttg ctgctccacc tacgacggtt gcaatcactt catctgttag
     8521 ttcgatttga ccatcttttg tattaatttt tacagtcatc ctttttacct caactagttg
     8581 atactctatt ttatcatatt tcagcccaag tgtaaaagca gaatgatgta tcagcggata
     8641 tttactctat ttttcaaacg atgttatacc cacaataaaa gaaaaaagac cctaaggtct
     8701 cctttgcttt tattattaaa cgcgttcaac tttacctgat ttcaaagcac gagctgaagc
     8761 ccaaactttt ttaggtttac catcgataag aacagtaact ttttgaaggt ttggttttac
     8821 ggcacgtttt gtttggttca tcgcgtgtga acggttgttt cctgatacag tcttacgacc
     8881 tgtaaagtaa catactttag ccattgtgtt ttcctcctat tagatctaat atagcggatg
     8941 tgctagcacc acataccgta ctatgttatc acactttctt gttttttgca agggaattgg
     9001 aagatttttt atttgtgtct taaatcaggt cttgcgtgac attttctgct ctccacatgc
     9061 catcgttgat taacagaaca ccagaattaa aattatgtgt ataaaaatca tctctaactg
     9121 cagctaaggg tatagccgtc aagtccaaat cccacagctc atctatcgat tttcttacaa
     9181 caatatctga atccaaatac agtacacgag actcgcttac atactttgga ataaaatacc
     9241 taaaaaagcc gcatatgaaa gtccctcaaa ggggagacga taacctttca gaatattact
     9301 gtcaatctaa acattcacaa tctcactatt caaagtctct agtctttttt ccatcaattg
     9361 gaaccattct cgcggaaggt catcattaaa aacataaaac ttaagattat aatgatgaac
     9421 acaaagagat tttattgttg tttcaacttt atccatataa gcattatctg cacctaagac
     9481 aatcgctttt ttctcttctt tcacttttta tctcatttct ttttattccc atcatatgtt
     9541 tctacgtaac cattattttc gcctattcgt tcgtaaaacc ataccagtgg agattttaga
     9601 tgaagtccca ttacggttta caatttttac attacgacac ggagttttac aaatcgattt
     9661 catttgccaa acgtagttag tgaggcagtt agctagttcg ccaaatagcg actagcgtcc
     9721 aacaatttgg aactttagtt ccaattgttg gtactgagtc acatcttctc ctctaactct
     9781 acgtctggat acttgtccgc aaaccagcgg agggcaaagt cattttcaaa gagaaagact
     9841 ggttggtcaa aacggtcttt ggctaagata ttgcgacttg acgacatccg ttcatccaag
     9901 tcctcaggct tgatccaacg aacggtcttt ttacccattg ggctcatgac cacttccgca
     9961 ttgtattcgc cttccatacg gtgtttaaag acttcaaact ggagttgacc aacagctccc
    10021 agcatgtact cacctgtttg gtaattctta taaagctgaa cggctccttc ttgcaccaat
    10081 tgctcaatcc ccttgtggaa ggatttttgc ttcataacat tcttagcaga aactttcatg
    10141 aaaatttcag gagtaaaggt tggcagtggt tcaaattcaa acttgttttt tccaaccgtc
    10201 aaggtatccc caacctgata agtaccggta tcgtaaaccc cgataatatc acctgccacg
    10261 gcattggtca cattctcacg actctccgcc ataaactggg taacattaga tagtttagcc
    10321 cccttaccag tacgagggag attgacactc atgccgcgct caaattcgcc tgatacgata
    10381 cggacaaagg caatacggtc acggtgacga ggatccatgt tggcttggat tttaaagaca
    10441 aagcctgaga aatccttgtc ataaggatcc acaatttcac cgtctgtttt cttgtgacca
    10501 tgtggttctg gagcaaactt gaggaagatt tcaaggaagg tctgcacacc aaagtttgtc
    10561 agggctgaac cgaaaaagac aggcgtcaat tctccagcca gaatagcttc ctctgaaaac
    10621 tcattcccag cttcatttaa aagctcaatg tcatccttga cttgctcgta gaaaggattg
    10681 ctaccaaaga gtttgtcccc gtcttctaga ctggcaaaac gctcatcccc tttataaagc
    10741 tctaaacgtt ggttatagag gtcatacaag ccctcaaagg ctttccccat cccgataggc
    10801 cagttcatag ggtagctagc aatgcccaag atttcttcca attcttgcaa gagatccaaa
    10861 ggctcacgac cgtcacggtc cagcttgttc ataaaggtaa agactggaat gccacgatgt
    10921 ttcacaacct caaacaattt cttggtttga gcctcgatcc ccttggcaga gtccacgacc
    10981 atgaccgcag catccaccgc catcaaggta cgataggtat cttctgagaa gtcctcgtgc
    11041 cctggcgtgt ctaagatatt cacgcgcttg ccgtcgtagt caaattgcat aacagatgaa
    11101 gtaacagaaa tcccacgttg cttctcgata tccatccagt cagatttagc aaaagtccct
    11161 gttttcttcc cttttaccgt accagcctca caaatctcac ccccaaagta gagtaactgc
    11221 tcagtgatgg ttgttttccc cgcgtccggg tgggagataa tggcaaaggt acgacgtttc
    11281 ttaatttctt cttgaatatt cataagttct ctttctttga ttctctattt ttcttgtttc
    11341 aatagctgag aatgattttt acattggatt ttaccattcc tttcaacact ccattatatc
    11401 ggattttagc atttttttca atttctattt cttttcactt ccccctccct tatttatggg
    11461 aaaatatggt aaaatagaac agactaaaaa tcatcatttc acgaaaggat gcaagatgaa
    11521 aattacgcaa gaagaggtaa cacacgttgc caatctttca aaattaagat tctctgaaga
    11581 agaaactgct gcctttgcga ccaccttgtc taagattgtt gacatggttg aattgctggg
    11641 cgaagttgac acaactggtg tcgcacctac tacgactatg gctgaccgca agactgtact
    11701 ccgccctgat gtggccgaag aaggaacaga ccgtgatcgc ttgtttaaaa acgtacctga
    11761 aaaagacaac tactatatca aggtgccagc tatcctagac gatggaggag atgcctaatg
    11821 acttttaaca ataaaactat tgaagagttg cacaatctcc ttgtttctaa ggaaatttct
    11881 gcaacagaat tgacccaagc aacacttgaa gatatcaagt ctcgtgagga agccctcaat
    11941 tcatttgtca ccatcgctga ggagcaagct cttgttcaag ctaaagccat tgatgaagct
    12001 ggaattgatg ctgacaatgt cctttcagga attccacttg ctgttaagga taacatctct
    12061 acagacggta ttctcacaac tgctgcctca aaaatgctct acaactatga gccaatcttt
    12121 gatgcgacag ctgttgccaa tgcaaaaacc aagggcatga ttgtcgttgg aaagaccaac
    12181 atggacgaat ttgctatggg tggttcaggt gaaacttcac actacggagc aactaaaaac
    12241 gcttgggacc acagcaaggt tcctggtggg tcatcaagtg gttctgccgc agctgtagcc
    12301 tcaggacaag ttcgcttgtc acttggttct gatactggtg gttccatccg ccaacctgct
    12361 gccttcaacg gaatcgttgg tctcaaacca acctacggaa cagtttcacg tttcggtctc
    12421 attgcctttg gtagctcatt agaccagatt ggaccttttg ctcctactgt taaggaaaac
    12481 gccctcttgc tcaacgctat tgccagcgaa gatgctaaag actctacttc tgctcctgtc
    12541 cgcatcgccg actttacttc aaaaatcggc caagacatca agggcatgaa aattgctttg
    12601 cctaaggaat acctcggtga aggaattaac ccagaggtta aggaaaccat tctaaatgcc
    12661 gctaaacact ttgaaaaatt gggtgctatc gtcgaagaag tcagccttcc tcactctaaa
    12721 tacggtgttg ccgtttatta catcatcgct tcatcagaag cttcatcaaa cttgcaacgt
    12781 tttgacggta tccgttacgg ctaccgcgca gaagatgcga ctaatcttga tgaaatctat
    12841 gtaaacagcc gtagccaagg ttttggtgaa gaagtgaagc gccgtatcat gctgggtact
    12901 ttcagtcttt catcaggtta ctacgatgcc tactacaaga aggctggaca ggtccgtaca
    12961 cttatcattc aagatttcga aaaagtcttc gcggattacg atttgatttt gggtccaact
    13021 gctccaagtg ttgcctatga cttggattct ctcaaccatg acccagttgc catgtactta
    13081 gccgacctct tgaccatacc tgtaaacttg gcaggactgc ctggaatttc gattcctgct
    13141 ggattctctc aaggtctacc tgtcggactc caattgattg gtcccaagta ctctgaggaa
    13201 accatttacc aagctgctgc tgcttttgaa gcaacaacag actaccacaa acaacaaccc
    13261 gtgatttttg gaggtgacaa ctaatgaact ttgaaacagt catcggactt gaagtccacg
    13321 tagagctcaa caccaattca aaaatcttct cacctacttc tgcccacttt ggaaatgacc
    13381 aaaatgccaa cactaacgtg attgactggt ctttcccagg agttctacca gttctcaata
    13441 aaggggttgt tgatgccggt atcaaggctg ctcttgccct caacatggac ctccacaaaa
    13501 aaatgcactt tgaccgcaag aactacttct accctgataa tcccaaagcc taccaaattt
    13561 ctcagtttga tgagccaatc ggttataacg gctggattga agtcgagcta gaagacggta
    13621 cgaccaagaa aatcggtatc gaacgtgccc acctagagga agacgctggt aaaaacaccc
    13681 acggtacaga cggctactct tacgttgacc ttaaccgcca aggggtacct ttgattgaga
    13741 ttgtatctga agccgatatg cgttcgccag aggaggccta tgcttatctg acagccctca
    13801 aggaagttat ccagtacgct ggcatttctg acgttaagat ggaagaaggt tctatgcgtg
    13861 tggatgccaa catctccctt cgcccttatg gtcaagaaaa attcggtacc aagactgaat
    13921 tgaagaacct caactccttc tcaaacgttc gtaaaggtct tgaatacgaa gtccaacgcc
    13981 aggctgaaat tcttcgctca ggtggtcaaa tccgccaaga aacacgccgt tacgatgaag
    14041 ctaacaaaac aaccatcctc atgcgtgtca aggaaggggc tgctgactac cgctacttcc
    14101 cagaaccaga cctacccctc tttgaaattt ctgacgagtg gattgaggaa atgcggactg
    14161 agttgccaga gtttccaaaa gaacgtcgtg cgcgttatgt atctgacctt ggtttatcag
    14221 actacgatgc tagtcagttg actgctaata aagtcacttc tgacttcttt gaaaaagctg
    14281 ttgccctaga tggtgatgcc aaacaagtct ctaactggct ccaaggggaa gtcgctcagt
    14341 tcttgaatgc tgaaggtaaa acactggaac aaatcgaatt gacaccagaa aacttggttg
    14401 aaatgattgc catcatcgaa gacggtacta tttcatctaa gattgccaag aaagtctttg
    14461 tccatctagc taaaaatggc ggtggcgcgc gtgaatacgt ggaaaaagca ggtatggttc
    14521 aaatttcaga tccagctatc ttgatcccaa tcatccacca agtctttgcc gataacgaag
    14581 ctgctgttgc cgacttcaag tcaggcaaac gtaacgccga caaggccttt acaggattcc
    14641 ttatgaaggc aaccaaaggc caagccaacc cacaagttgc ccttaaacta cttgcacagg
    14701 aattggcgaa gttgaaagaa aactagacag aacaaaacca gccctaaggt tggttttttc
    14761 ttctctacca actcccaata actattttgg ctttatttcc agagtatttt atggtaaaat
    14821 gaagagtaat aatatttatt aaagaggtaa aaacatgatt gaagcaagta aattaaaagc
    14881 tggtatgacc tttgaaacag ctgacggcaa attgattcgc gttttggaag ctagtcacca
    14941 caaaccaggt aaaggaaaca cgatcatgcg tatgaaattg cgtgatgtcc gtactggttc
    15001 tacatttgac acaagctacc gtccagagga aaaatttgaa caagctatta tcgagactgt
    15061 cccagctcaa tacttgtaca aaatggatga cacagcatac ttcatgaata cagaaactta
    15121 tgaccaatac gaaatccctg tagtcaatgt tgaaaacgaa ttgctttaca tccttgaaaa
    15181 ctctgatgtg aaaatccaat tctacggaac tgaagtgatc ggtgtcaccg ttcctactac
    15241 tgttgagttg acagttgctg aaactcaacc atctatcaaa ggtgctactg ttacaggttc
    15301 tggtaaacca gcaacgatgg aaactggact tgtcgtaaac gttccagact tcatcgaagc
    15361 aggacaaaaa ctcgttatca acactgcaga aggaacttac gtttctcgtg cctaatctct
    15421 agaaagaggt cattctatgg gaattgaaga acaacttggc gaaatcgtta tcgccccacg
    15481 tgtacttgaa aaaatcattg ctatcgctac tgcaaaggta gagggtgttc actctttttc
    15541 aaacagatca gtgtctgata ccctttcaaa actttcactc ggccgtggca tttatcttaa
    15601 aaacgtggac gaagaactca cagcagatat ctatctctac cttgagtacg gagtaaaagt
    15661 tcctaaggta gcggttgcta tccagaaagc tgtcaaagat gccgtccgta atatggctga
    15721 tgtagaactc gctgctatca atattcacgt tgcaggtatc gtcccagata aaacaccaaa
    15781 accagaattg aaagatctat ttgacgagga cttcctcaat gactagtcca ctattagaat
    15841 ctagacgcca actccgtaaa tgcgcttttc aagctctcat gagccttgag ttcggtacgg
    15901 atgtcgaaac tgcttgtcgt ttcgcctata ctcatgatcg tgaagatacg gatgtacaac
    15961 ttccagcctt tttgatagac ctcgtttctg gtgttcaagc taaaaaggaa gaactagata
    16021 agcaaatcac tcagcattta aaagcaggtt ggaccattga acgcttaacg ctcgtggaga
    16081 gaaacctcct tcgcttggga gtctttgaaa tcacttcatt tgacactcct cagctggttg
    16141 ctgttaatga agctatcgag cttgcaaagg acttctccga tcaaaaatct gcccgtttta
    16201 tcaatggact gctcagccag tttgtaacag aagaacaata aaaagaaaaa gtgtttgata
    16261 gatatcaaac actttttctt tgcctcccac tatctaaaaa aatgataata gatataattg
    16321 taaacaaaaa tccagatagg ttttgcatga ttgagaaagt taaaaaacta tggcagagaa
    16381 tcgttaatct cagattgtcg gtagaacgat aaacaagggc aaaaaagaaa ccaatcaaac
    16441 tataatataa taaactaatt ggatctctgt gagatagtat caaatgacta agcccaaaga
    16501 taaggcctga taagatgaca tctaaatagt actttgactt aggaaagaag gtattcataa
    16561 aataccctct atcaagagtc tcctcaaaaa caggatcgat gattacaggc aggacaaaag
    16621 ataagatagt cgataaaaag gttggttgtc catttgaaag aaccacggta aaatactcat
    16681 catgaatatt cctatgatta atcaaatgag catagcgtgc ccaaaaatta ccgagaatct
    16741 gataaaccac ataagttgca aataagtaga agacaaatga ccagttccag ctctttttct
    16801 caaagataaa gagcatcttt tcttttttaa cctccaaatt aatagaagga aacttcccac
    16861 taatcccatt gttaaaataa gagaatagac atcagctcct aaccctaaaa tgatcgtcac
    16921 atacaatcca attgtttgtg gtaaataggt agatagtaaa ataataagca aaaatattcc
    16981 aaattgtctt agtttctttg tgtttctcat cgtacttttt tgaaagatta ccctgttcgg
    17041 aagccgtact tccaagcatc tatataagaa ttaagtgccc cttgcctcat ataaggagca
    17101 aattctctat aatataacca tctactatat ccatcttccc aaacagcaag accacctgaa
    17161 gtttgctcca agtcctcagt tgaaataact gtaaatgttt ctgtacctgt cattgcaagt
    17221 accttcttaa aatatagtga tttgaaataa gatgtgaaca actctatcag gaaagtcaaa
    17281 ttaatttata gaaatatttt agcagccaag gtgtactgtt atagattcaa tacactataa
    17341 atttttgtaa gcttacatct acagtataca tcttttttgt ctattttata gcccatctcc
    17401 tcaactgtca atttttcaac ctgaattgca tttttccata aaaaatgaga cctttctagt
    17461 ctcatttagt cattcttagt attttctaaa tcgttgatag cgttcttcca gcaactcttc
    17521 tagcggtttt tgtgaaagtc tagccagctc cgtttggagt tcttttttga cactcttaat
    17581 cagttcttta ctagaaagtc ctacttcaga aatcacctta tccaccacgt ccatttctaa
    17641 cagttcatgc gaagtgattt tcatcagttc tgctgcttcc atggcgcgag taccgtcctt
    17701 ccataaaatg gaagcaaagc cttctggact gagaatggca tagatagaat tttccagcat
    17761 ccagacacgg tccgcgacag ctagagccag agccccgcct gaaccacctt caccgataat
    17821 aatggcgata ataggaactt tcaggtcact catttccatg agattgcgag cgatagcttc
    17881 cccttgacca cgttcttccg ctccgacacc aggataagca cctgctgtat tgataaaggt
    17941 cacaactgga cggccaaatt tctcagcctg tttcatcaac cgcagtgcct ttcggtagcc
    18001 ttctggatgt ggttggccaa aatttcgttt gaggttgtct tgcaaactct tgcctttttg
    18061 gataccaacc actgttacag cttggtctcc aagccaacca ataccaccaa caactgcacc
    18121 atcatcacga aaagaacggt caccatgtaa ttggataaat tcatcaaaaa tgcctgtcgc
    18181 aaagtccaag gttgtcaagc gactctgctc acgcgcttct ctgactattt ttgcaatatt
    18241 catctaggac tccctccatg caatctgact aggctagcaa tcgtatctgg taagtctctt
    18301 cttttgacaa tagcatccac aaagccatgt tctaatagga attctgcctt ttggaaatcc
    18361 tcaggcaagc tttcacgaac cgtattttca atcacacgac gtccagcaaa accaaccaag
    18421 ctctgtggtt cagccagaat gatatcgcct tccatagcga aagaagctgt cacaccacca
    18481 gtcgttggat ctgtcaaaat ggtcaggtaa aagagaccag catttgaatg gcgtttaacc
    18541 gccgcagaga tcttagccat ctgcatgaga ctcatgattc cttcctgcat acgggctcca
    18601 ccagaggctg tgaataggac aactggcaat ttttcgacag tcgcatactc aaacaaacga
    18661 gtgatttttt cacctacaac cgtacccata gaagccatga taaagttaga atccataatc
    18721 ccaagagcca cagtctgacc tttaataaga gcagttcctg tcacaacggc ttcatgcaga
    18781 cctgtttttt cacgcataga tgccagtttc ttttggtaac cagggaaatg caagggatcc
    18841 ttgctttcaa tccctgtaaa caattctttg aaggttccca tatcaatcgt caaagccaag
    18901 cgttcttggg cagaaatacg aaaggtatag ctacagtgcg gacagatacg ttcacttccc
    18961 agatccttct gatagatggt atgcttacag cctggacact gggaaaataa ttcatctgga
    19021 acctctggct tagcttgagg tttttcccta accgaacgat tgggattgat tcgaatatac
    19081 ttatcttttt tactaaatag agccattgat tccccttttc ggtttaaact cttaaagtca
    19141 ttttattctt tttcttgata tttaggtaag aaggtttcca tcaagaagga agtatcataa
    19201 tccccagcaa tgacattgcg atctgaaatg aggtcaagct ggaaatctgc attggtctgc
    19261 actccttcaa tttctaattc atagagggca cgttgcattt tcatcaaggc gtcaaaacga
    19321 ttttcgccgt gtactatgat tttggcaatc atactatcat aataaggcgg aatggtataa
    19381 cctggataaa ctgctgaatc cacgcgcaag ccaactccac cacttggcag atagagatta
    19441 gtaatcttac ctggacttgg agcaaagtta aaggctgggt tttctgcatt gatacgacac
    19501 tcgatggcat gaccgcgtag gacaatatct tcttgcttaa cagacaaagg ctgacctgcc
    19561 gcaatgcaaa tctgttcctt aacgatatca acacctgaaa caaactctgt tactggatgt
    19621 tctacctgaa cacgagtatt catctccatg aaatagaaat tgctacttgc ttcatcaaga
    19681 agaaattcaa tggttcctgc attctcatag ccaacaaact ctgccgctcg aacagcagca
    19741 gcacctattt catgacgcag cgtttttccg attgcaatcg agggactttc ttccaaaacc
    19801 ttttggttat tcctttgaag agaacaatcc cgttcaccca agtgaatcac atgtccatgc
    19861 tcatcaccta ggatttgaac ctcaatgtgc cgagctggat agataacccg ttctatgtac
    19921 atggcaccat tgccataatt ggccttggcc tcactagagg cagtttcaaa ggcagaaacg
    19981 aggtcatctg gtttttcaac cttacgaatc cctttaccac ctccacctgc tgaagccttg
    20041 agcataacag gatagccaat tttttcagca acaatcaaag cttcttcaga gttatgcact
    20101 tctccatctg aacctggtat aacaggcaca cctgctttaa tcatctgagc acgcgcattg
    20161 atcttatccc ccatcatatc cataacatga ccagatggac cgataaactt gatacctact
    20221 tcttcacaca tggtcgcaaa tttggaattt tcactgagaa atccaaaacc agggtgaata
    20281 gcttctgcct cagtcaagac tgcagctgat agaactgcat taatattgag ataagactct
    20341 gttgccttgc caggaccaat acaaactgct tcatctgcca acagcgtatg aagagcttcc
    20401 ttatcagcag ttgaataaac cgctaccgtc gcaatcccca attcacgtgc cgcacggata
    20461 atacgaaccg caatttcacc acgattggca attaaaattt ttcgaaacat ggagaacctc
    20521 cttagttccc aattgcaaaa gtaagggtac cactggctgc aagcttgcca tccacttcag
    20581 cctttgcttc aaccacagct atggtgccac gacgttttac aaaagtcgct gtcataacca
    20641 attggtcgcc tggtacaact tgcttcttga acttaacctt atccatacca gcgtaaaaga
    20701 ccagttttcc tttattttca ggttttgata actccaacac accagcagtt tgcgccaagg
    20761 cttccataat cagaacacct ggcataactg ggtattgagg aaagtggccg ttaaagaaag
    20821 gctcgttgat ggtcacattt ttgatagcaa caatggtatc ctcgctcacc tccaagacac
    20881 ggtccactag aagcatagga taacggtggg gaagggcttc tttgattcct tgaatatcga
    20941 tcatttgata cgtaccaatc ctttaccaaa ctcaaccatt tcttcgttag agacgagaat
    21001 ttccgttacc acaccatcct taggagctgg gatttcattc atgactttca tggcttcgat
    21061 aattaccaat gtttgacctt ttttgacact atcaccaact gtaacgaagg caggtttatc
    21121 tggtccagca gccaagtaaa ccactccaac aagtggactc tctacaagat ttccctcact
    21181 agccacactt gcttcagctg gagctggaac ttcttctgct acagtctctg ctggagcaga
    21241 tgtaggagct actggactcg gtggtgctag aacgggtgct ggagcgactt gagttgcaac
    21301 ttcaggcaca ggtctcgctt cattcttgct aaactgcaac tcatccgtcc catttttata
    21361 agaaaattct ctcaaacttg actggtcaaa ttgagtcatc aagtctttaa tatcgtttaa
    21421 attcatactt atctattctc ccaacgtttg aaagcaagaa ctgcattgtg gcctccaaaa
    21481 ccaaaagtat ttgaaatagc gtatggaatt tctttctcca agccttgtcc ataaacgaca
    21541 ttagcttcga tataatctga tacttcactt gtcccagctg tcattggtac aaagttatga
    21601 cgcatagctt cgatggtgac gatagcttct actgcacccg cagcccccag caaatgtcct
    21661 gtaaaagact tggttgatga tacaggtact tccttaccaa gaacagctac gatagcacca
    21721 ctttctcctt tttcattggc aggagttgac gttccgtgag cattgacata ggctacttgc
    21781 tctggagaaa tctcagcttc ttccaaggct agtttgatgg ccttgatagc tccctgacct
    21841 tctggatgtg gagaagtcat gtggtaggca tcacaagtat ttccgtaacc aaccacttca
    21901 gccaggatag tagctccacg tttttcagcg tgttcaagac tttctagaac caacatccct
    21961 gaaccttcac ccataacaaa cccattgcga tccttatcaa atgggatcga agcacgagtt
    22021 ggatcctctg tagtagagag agctgttaag gcttggaaac cagcgatggc aaaaggtgtg
    22081 atagaagctt ctgttcctcc caccaacatc acatcttgga aaccaaactt aatggagcgg
    22141 aaggcatccc caatcgcatc atttgatgaa gagcaggcag tattgataga tttacaaaca
    22201 ccgtttgcac caaaacgcat ggctacattc ccagaagcca tatttggtaa agcttttgga
    22261 agagtcattg gtttgacacg tttgggtcct ttttcatgaa ggcgaagtac ctgatcttca
    22321 atttccttga ttccaccaat accagatgca acgataacac caaaacgatc cctattaaga
    22381 gcctctacat caagattggc atgatttaca gcctcttggg ctgcatacaa ggcatataaa
    22441 gaatagttat caaaacggtt ggtatctttt tttacaaagt atttatcgaa cggaaaatct
    22501 tggatttctg ccgcattatg cacatcaaag tcactatgat caaattttgt aatgccacca
    22561 atgccgattt tcccagttgc taaactattc caaaattctt ctggtgtatt tccgattgga
    22621 gatgttactc cataacctgt taccactact cgatttagtt tcattctttt cacctctagc
    22681 tttcgctaca tacttaagcc accatcaatg gcaaccactt gaccagttag ataatcttgg
    22741 cctgctaaaa atactgtcaa atctgcaacc tgctctgcct gcccaaattc tttcatcgga
    22801 atctgagcta gtgtagcttc cttaatctta tctgataaga tagctgtcat atcagactca
    22861 atcattcctg gagcaatcac attgactcgt atattccgac tagcgacctc gcgtgccaca
    22921 gacttggtaa agccaatcaa gccagcctta gaagcagcat agttagcttg accaatattc
    22981 cccatcaaac caacaacact agacatatta atgatagcac cttctctggc tttcatcatc
    23041 ggtttcaaga ctgattgtgt catattaaag gcaccagtca gattgacctt gagcactttt
    23101 tcaaaatctg cttctgtcat cttgagcata agagtatctt gggtaatccc tgcattgttg
    23161 accaaaacat ctactgaacc cagttctgca atagcttgat caatcatacg cttagcgtct
    23221 gcaaaatctg atacatctcc tgaaatggga accaccttga taccatagtt tgaaaactca
    23281 gcgagcaatt cttctgagat tgccccacga ctgtttaaga caatgttggc tcctgcttga
    23341 gcaaacttgt gggcgatggc aagaccaatt ccacgactcg aacctgtaat aaagatattt
    23401 ttatgttcta gtttcatttt tttcctttca aaacttctac ttattttagt ctatttttct
    23461 aaaagtgcta ctaaactcgc ttgatcttcc acatgagcta agtgagcagt ttgatcaatt
    23521 tttttaacaa aacctgacaa gactttcccc ggtccaatct cgataaagtt gcttatgcct
    23581 gcttcttgca tgaccccaat actttcatag aaacgaacgg gttccttgac ctgacgcgtc
    23641 aagagctgag caatgtcctc tttttgcatc acagcagctt ctgtattgcc gactagggga
    23701 caagtaaaat ctgaaaaact tacctgagct agagtttcag ctagtttctg gctagctggc
    23761 tcaaggagag cggtgtgaaa gggacctgac accttaagag gaatcaagcg tttggcacct
    23821 gcttcttgca aaagttcaac cgctcgatca actgcaacca cttctccagc aatgacgatt
    23881 tgtgcaggtg tgttatagtt ggctggagta accactccaa gttcagaagc tttttgacag
    23941 gcttcttcaa tgacctctac tggcgtattg agaactgcta ccatcttgcc agagtcagca
    24001 ggagccgctt cttccatata ggctccacgc ttagctacca aggcaaccgc atcttcaaaa
    24061 tccaaggcgc cgcttgccac caaggcagag tattctccaa gagacaaacc agcaaccata
    24121 tcaggctgat agcccttttc ttgcaataaa cggtagatag caaccgaagt cgctagaatg
    24181 gctggttgcg tatagcgggt ctgattgagt ttgtcttctt ccgtatcgat gagataacgc
    24241 aaatcataac ctagcacctg actcgctcga tcaatcgttt ctttaacaat cggatactga
    24301 tcatagaaat cccgtcccat ccctagatac tgggcacctt gaccagcaaa taaaaaggct
    24361 gttttagtca tttcttacaa ctcctgtcca gcgagaggct tcttcttgaa ttttcttagc
    24421 ggctccgtaa tacaaatctt ttaggatttc ttcagctgtt tcttctttag aaacaagccc
    24481 tgcgatttga cctgccatga cagagccacc ctccacatca ccgtgaacaa ctgctttggc
    24541 tagggcaccg gctcccattt gttcaaagat ttctaaatca ggatcttcct gcttaaaggc
    24601 atctttttca gccagttcaa aatctctagt caactgattt ttaatagcac gaacagcatg
    24661 accaaagtgc tgagctgaaa tcgtagtgtc aatatccctt gcttttaaaa ttttctcctt
    24721 gtagtttgga tgggcattcg actcttttgc aactacaaac cgtgtcccca cctgtacagc
    24781 ctctgcacct agcataaagc cagccgcagc accttcacca tccgcaattc ctcctgcagc
    24841 aataacagga atagatacag ctgtggctac ctgtcgcacc aaggtcatgg ttgttaattt
    24901 accgatatgc cccccagctt ccattccttc tgcaataaca gcgtctgcac cgattttttc
    24961 catgcgttta gctaaagcga cactaggaac gacaggaata acgattatcc cagcttcatg
    25021 gaaacgttcc atatacttgc ttggatttcc tgctcctgtt gtgacaactt taacaccttc
    25081 ttcaataacg agatccacga tatcttccac aaagggagat aagagcatga tgttgacccc
    25141 aaagggttta tcagtcaatg atttgatttt atcaatattg gccttgacaa cttctttcgg
    25201 ggcatttccc ccaccgataa ttcctaatcc tccagccttg gaaacagccc ctgccaaatc
    25261 accatcagca acccaggcca tccctccttg gaaaatagga tagtcaatct tcaataattc
    25321 tgtaatacgc gttttcatag tgcctccaac cttccttgct tacgtaatag ttcgatttca
    25381 ccataatttg acagtcaaac tattacctaa acaagaggga gtgggtttct ccctactcct
    25441 tctactaata ttctgcttat tttgcttgct cttcaacgta agcaaccaag tcaccaactg
    25501 ttttcaagtc attttctgct tcgatttgga tatcaaaagc atcttcgatt tctgagatta
    25561 cttggaacaa gtccaatgaa tctgcgtcca aatcatcaaa agttgattca agtgttactt
    25621 ctgatgcgtc ttttccaagt tcttcaacga taatttcttg tactttttca aatactgcca
    25681 tgataggact cctttaaaat aaatagtttt tttataacaa tgtgttcacc acatgattac
    25741 ctaaattgta agaatgagcg tgccccaggt caagcctcca ccgaagcctg atagaagaac
    25801 agtctggcta ccatctaaag ggatgagacc ttgttctaca cactctgaaa gtaaaatcgg
    25861 gatactggct gcactggtat tgccatattc catcatattg gctggaagtt tggctcggtc
    25921 aacaccaatt tttctagcca tcttatccaa aatacggtca ttggcttgat gaagtagcag
    25981 ataatccaag tctgtcacct ctataggaga ttcatcaata gtctgcttga tagacttggc
    26041 tacatctcga atggcaaaat caaagactgt gcgtccatcc atcttcaaaa atgaatctgc
    26101 actttcttga tctgaaaatg gagaatgcaa acctgaatgc ccataagtta aacactcgct
    26161 gcgacttcca tcgctattga gactctcagc taagaaatgc tcttgctcgc tagcttctaa
    26221 caagacacca ccagcaccat ctccaaacaa cacagctgtt gatcgatccg accaatcgac
    26281 tgccttagag agggtttcac taccaatcac caagcctttt tgaaagcgac cagaagcgat
    26341 aaacttttca gcagttgaaa gagcaaatac aaatccactg caagccgcgg ttaagtcaaa
    26401 agcaaaggct ttattagcac caatattagc ttgaacacga gcagctgtag agggcatcat
    26461 cgaatctgga gtaatggtag ctaggatgat aaaatccagt tcttctcctg ttattccagc
    26521 ttttgccatc agtttcttag caacctctgt agccaaatca ctggtagatt ctgttcttga
    26581 aatatgcctt tgtcgtattc ccgttcgact tgaaatccac tcatcattgg tatccataat
    26641 ctgagccaag tcgtgatttg taaccacttg ctctggcaca taatgagcaa cctgacttat
    26701 ttttgcaaaa gccattattt caaatcctcc aaaaattggt aaagattagt caagccttta
    26761 cccatgacag caatttcttc ctcgctcatg ccatcaataa ttttttctac catggccttg
    26821 tggaagcgtt tatgcagtct atgaatcaag cgacccttct ttgtcaaatg cagatgcacc
    26881 acacgacgat cctgttctga ccgaactcgc tcaatgtagc ccttacgctc taaattgttc
    26941 aaacttgtcg taacagttcc aagagttacc atcaactctt ttgacacttg acttggagtc
    27001 acatctggag ccttaccgat gacatcaatc gtatgcattt ctttgatgga gatatcctta
    27061 aaacgactac ctctcaagtt cacttcctca attacaagga cattgttaaa tatagatgtt
    27121 aaatattcat tgattcgttg gtagtccatt tttcatatcc ctccttcttc aaaaaacttt
    27181 tacaatcaaa acatttgaca gaaagaatta tatcaaaatt caaagaatga tgcaagtatt
    27241 ttttattttc ctgtaaattt aggtcttctt ctctccgaat gagctcgcac tccttctttg
    27301 aaatcctctg tttgagctaa ggatttctgt aggttcagtt ctaaagtagc gtaaccctgc
    27361 cagtctttaa attggctctc ccaaaccaac ttcttaatgg cagcatagga attacttgaa
    27421 gcacgtctta attttttaag aagctgttct ctcgtctttt caagtttttc agcttcagaa
    27481 acgcggtaaa ccaaccccca ttctagggct ttttctgccg ttaaagcctc tcctgtcata
    27541 gctaattgag cagcacgcgt cacaccgata ctgcggctca agagatgaat tccccctgca
    27601 tctggagcca aaccaacacc aacaaaggct tggataaact tagccttatc cgtcgccaaa
    27661 cagaaatctg ccgcaacagc catattcgct gcggcacctg caacagcacc gtcaacttcc
    27721 attaaaacag gtttggctat ttgcttgatt ttataagaaa tcgtattgac caactctgcg
    27781 atttttgtca atgatggaat atcatcctca tccactgccc gcttcatctc taccaaatct
    27841 cccccaactg agaagacctt cccattggca ttgattaaga taaaatgcac agctggattt
    27901 tcttctgcca aagtcagagc ttctaaaatc tcctcacaca tgggaatatg aaaaccattt
    27961 gcgacctcag gacggttcaa ggtaaggatt gccaagtcct cttcaagctg ataaataatg
    28021 tgttccatca gggctccttt ttattttata agcagtttat actcttcgaa aatctcttca
    28081 aaccgcgtca gcttccatct gcaacctcaa aacagtattt tgagctgact tcgtcagtct
    28141 tatttacaac ctcaaagcag tgctttgagc agtctgcggc tagcttccta gtttgctctt
    28201 tgatttcatt gagtgttaaa ttatttcact gtcaaagtat atctttttta ggagcagaaa
    28261 gcaagaaaaa atcgactgaa agtgtctcag ccgataagaa aggctcgctt taggctagaa
    28321 agcatcttat tttcttcatt gaaaaaagcc tttctttctg ctataatcgt ataaaatact
    28381 tactttagga gttcttatga aagttgttaa atttggaggt agttctcttg cctctgctag
    28441 tcaattagaa aaagttttaa acatcgtcaa aagcgattca gagcgtcgtt ttgtagtcgt
    28501 ttctgcgcct ggtaaacgca atgctgaaga tactaaggtt acggatgccc tgattaaata
    28561 ctaccgcgac tatgttgcgg gtaacgatat tagcaagagc caaagctgga ttatcgaccg
    28621 ctatgctgct atggttagtg aattgggact aaaaccagct gtgctagaaa aaatttctaa
    28681 aagtattcac gccttggcca ctcttcctat tgaagaaaat gaatttctct acgatacttt
    28741 cctagcagcc ggtgaaaata acaatgccaa attgattgct gcctacttta accaaaatgg
    28801 tatcgatgca cgctatatgc accctagaga agctgggatt gtggtcacaa gtgaacctgg
    28861 tcacgctcgc atcattccat caagttatga caagattgaa gaattgacaa acaccaatga
    28921 agtccttgtc attcctggtt tctttggtgt cactaaggaa aatcaaagct gtactttctc
    28981 acgtggaggt tctgatatta caggttctat cattgctgct ggtgtcaaag ctgacctcta
    29041 tgaaaacttt acggacgttg atggtatctt tgcagcccac cctggtatta tccaccaacc
    29101 acactcgatt cctgagttga cctaccgtga aatgcgcgag ttggcctatg caggcttctc
    29161 agtccttcat gacgaggctc ttcttcctgc ctaccgtgga aaaattcctc tggttatcaa
    29221 gaataccaac aaccctgacc atccaggtac tcgtatcgtt ctaaaacaca gtaatgatga
    29281 atttccagtt gtgggaattg ctggtgactc aggctttgtc agcattaaca tgtcgaaata
    29341 cctcatgaac cgtgaggttg gatttggccg caaggttctg caaatcctgg aagaacttaa
    29401 catcggttgg gaacatatgc caacaggtat cgacgatctt tctatcattc tccgttctcg
    29461 ccaactaact cccatcaagg aagaagaaat cctgcgtcag ttggttcaaa aggctaaagt
    29521 agatcatgca gaaatcgaac acgacctttc tatcattatg attgttggtg aaaaaatgaa
    29581 gagccacatc ggagtgactg ctactgcgac acgcgctcta tctgaaaaca aaatcaacat
    29641 ccagatgatg tctcaaggtt ctagtgaagt ttctattatg tttgttgtca ataaagacca
    29701 agagaaagca gcaattaaag ccctctacaa tgcctttttt ggagaaagta aggaagacta
    29761 agcatggccc aatcacttaa caaaacagta ctcctcagta cgacaggcac ttcctacctc
    29821 tctatcgctg ggaaagttgg gaaattcctt gtcggagatc aggctttgga attttaccca
    29881 gatgtcaatg tcgaacaatt tatccagatt ccttggagcc atatcaatca gattggagcc
    29941 aatgtcactg gacgcaaaat cagccgccac ttcgaagtct ttacagacaa aggaaaattc
    30001 ctctttgctt caaaagactc aggtgccatt ctcaaaattg cacgcgaaaa actaggcaat
    30061 gacaaggtcg tcaaacttcc gaccctgatt cagacaatta gccaaaaatt taaaaatcta
    30121 tttgcaaaaa agtagaaact ttagtataat catagcaacg gataagtagg ttatcttctt
    30181 ctttcagaaa gtctgcgggt actgcgagca gataggaggg ataggtgaaa ttctaccgtt
    30241 agtaacaatt acatacacaa tcaagtgcac acctgtgaag ttggatggaa ccgtggccct
    30301 gccactccaa cgttttgtca ggcgtgcttt tttcataaag gagttcttat gttagatatc
    30361 aaacgtattc gtacagattt tgaagctgtc gcagaaaaat tagctacacg tggtgtagat
    30421 gctgctgtct tgaatgaaat gaaagaaatc gatgctaaac gtcgtaacat cttggtcaag
    30481 gttgaaactc tcaaagcaga acgtaacaca gtttctgctg agattgccca agctaagcgc
    30541 aacaaggaaa atacagatga caagattgct gccatgcaaa atctatctgc tgaggttaaa
    30601 gccttggatg ctgaattggc agaaatcgat gctaaattga cagaatttac aacgactctt
    30661 ccaaatatcc cagctgacag cgttcctgtt ggggctgacg aagacgacaa tgtggaagtt
    30721 cgccgttggg gtactccacg cgagtttgac ttcgaaccta aagctcactg ggatctcggt
    30781 gaagaccttg gtatccttga ctgggaacgc ggtggtaagg taacaggcgc tcgcttcctc
    30841 ttctataaag gcctcggtgc tcgtttggaa cgtgctatct acaactttat gttggatgaa
    30901 catggaaaag aaggctatac tgaagtcatc acaccttaca tagtcaacca tgattctatg
    30961 tttggtactg gtcagtatcc aaaatttaag gaagatactt ttgaactcag cgataccaac
    31021 tttgtcttga ttccaactgc tgaagttcct ctgacaaact actaccgtga tgaaatctta
    31081 gacggcaaag atcttccaat ctacttcact gctatgagtc cgtcattccg ttctgaggct
    31141 ggttctgccg gtcgtgatac gcgtggcttg atccgtttgc accaattcca caaggttgaa
    31201 atggtcaaat ttgccaaacc agaagaatct tacgaagaat tggaaaaaat gacagccaac
    31261 gctgaaaaca ttcttcaaaa actcaacctt ccataccgtg tcgttgctct ctctactgga
    31321 gatatgggct tctcagctac gaagacttac gacttggaag tgtggattcc agcacaaaac
    31381 aattaccgtg aaatctcaag ctgttcaaac acagaagatt tccaagcccg tcgtgcccaa
    31441 atccgttacc gtgatgaagc agatggcaag gtgaaactcc ttcatacctt gaacggttct
    31501 ggacttgcag ttggacgtac agtggctgca attcttgaaa attaccaaaa tgaagatggt
    31561 tctgtgacca tcccagaagc acttcgtcca tacatgggtg gagctgaagt catcaaacca
    31621 taaaaaataa ggtttagcta tttctagcta gacctttttt cgtaaccaaa tcagataagc
    31681 acctagtaca aagaataaaa tagttaggca tataatggtt tcagtcaata ccaggtaatc
    31741 cagaaatgga agtttcaaaa ttccctgagc catcttgagc gaggtcgctg tgataatggt
    31801 tgggaaggtg agggctgaga aggctggttg aaaaccttgt tttaaaatgt tgggcagacg
    31861 agttaaaaca aagaaaaaga aggattgaga agccaaaatc atgacaatca agacccaagt
    31921 cggcaggctg gttcctccta ctcgaactag agaagccaag agtagagaga aaggagcaca
    31981 gtagattcct tcttgtccaa gcaaggctag tgggagtgga tgtttcttta aatcgctata
    32041 aataagggga tagagataga aggtcaagag aaaaccaaaa ctcaaggtcg cataggcaat
    32101 ttcgataata cctaccagag gataggtcaa ggcagccact gctatcccca catagagaac
    32161 cgtccaactt ggagtggcat gaaccctccg ccctggacaa gcaaacttga tggtaaaacc
    32221 agcaatcaag gtcaaatcca agagaaatga aaaccaccaa atcccttgtg ctaccaaagg
    32281 aagataagag aatacgcgaa agacataggt cgataaaatc atcccagcca taggaaaggt
    32341 tgccattcct gacaaaagag ggggcttggt caattcttgc ttggtttctt tccaattaaa
    32401 gagatgcaga attagaaagt aaatccataa aaccaaacca gtcagactaa aaagatggga
    32461 tagaaccggc aacgtatcta aaataagatt tccagctcct gccaaaccta gcaaacaacc
    32521 tgaaaatact aaggggagtt ttttcatcct aacctccaat aatcatgtta gtttcagtat
    32581 aacataaaag cgcttaaatg aggatttaaa aaaacgaagt ccgcttattt cagacttcat
    32641 tttactcaga tatgaattag gcataaggtt gcaattctgg attaattggt gtattagcta
    32701 agttgttggc atagttacag aggattgcta ggctgacacc aaaaaccaca tccaaggcat
    32761 tttgttgagt gtagccagct tctaaaaact cagacaaggc ttcatctcct acacgaccct
    32821 tggtattgat aactgccaag gtaaacttag ctagggtatc caatttagga tctgtttcaa
    32881 ttggagtacg attgcgaaga gcttgaatca agtcatcatt catctggatt tgtttgatgg
    32941 aaaaggctgt gtgacctgcg acacagaagg cacaaccatt ggtcacggct gccgtgattt
    33001 gcaccacttc acgctcaacg ggtgtcaggc tgttgcgacg gtggatagat gagacaattt
    33061 ggtaggcttc taaaacagtc ggggcattgg ccaagagacc gattaggttg ggaatatagc
    33121 cattgttgtc tttttctact gtttcaagaa tttctttcac ttctgctggt gctgactcta
    33181 ctgtatggat agtaaatgtt gtcataagat acctcttttc ttattattga cactaatatt
    33241 attggaaaat cttataaaat cctgattcct aagtttatct aagataaagc tttattctct
    33301 cataagattt tcgttgttat attagtttat cacacttcca atcacttgca taatatatat
    33361 tatatatcag gctgataaaa attatttata ggcaaaaaaa tcacacgagc tgtgtgattc
    33421 cattatttgt caaaatactt tttagtttca gcaataacga ctggcgacaa gaccaagagg
    33481 gcaatcaagt ttggcagagc catcaaggcg ttaacgatat ctgcgataat ccagaccata
    33541 tccaactcga taaatcctcc taacaagacc atgagcacaa aaaccacacg gtagagccag
    33601 ataaagcgag ccccaaagag gaactcaaaa cagcgttctc cgtaatagtt ccaacctaga
    33661 atcgttgtaa aggcaaaaag tacaaggaag atggtcaaga gggcaggccc aaagtgtgaa
    33721 aagactgttg agaaagctga ctgagtcaag gcaaccccat tcaagtcacc actccaaact
    33781 ccagttatca agatggtcaa accagttaga gtacaaatga tgagggtatc aataaaggtt
    33841 cctgtcatgg aaatcaaacc ttgctctact ggttcatttg tcttggcagc tgcagctgca
    33901 ataggagcag aacccagacc agattcgttt gagaacacac cacgcgccac accattttga
    33961 atagccatcc gaacgctagc accagcaaat ccacctaccg cagcaagggg actaaaagct
    34021 gaggtaaaga ctaaagcgat tgtgccaggg atttttccga tattaaagaa aataactgta
    34081 agagttccta agatataaat gagggccata aaaggaacaa cagtagttga aaccttagaa
    34141 atagacttga gtccaccaaa gactgcaatc gctacaaaga cagacaagac gagagctgtg
    34201 atggctggcg aaatcgtcgt tgtattttgg atagattctg taatcgagtt gacttgggtg
    34261 aaggttccga ttcccaagag agcaaccaat actcctgcta ctgcaaacaa aacagcaagt
    34321 ggtcgccact tttctcccat ccctagaagg atatagtgca tgggacctcc cgctactgca
    34381 ccatggtcgt ccttggtgcg gtatttgatg gccaagagtc cttccgcata cttggtagcc
    34441 attccaaaga aagccgccat ccacatccaa aatagagctc ctggtccacc aaccttgata
    34501 gccgtcgcaa ctcctatgat atttcctgtt ccaacagttg atgccaaggc tgtacacaga
    34561 gctgcaaaac tggatacatc accatgtccc ttatcctgga taaaaataag ctgaaaggcc
    34621 ttgggcagac gcaaaacctg caagagtcct agccgcatgg ttaggtaaat ccctgttccg
    34681 accaataaaa tcaagagggg cggtccccaa gcaaaagcat cgattgattt aagcaattct
    34741 aacatttcct tctcctatcg tttcaacccc aaaagaaaga gcacatgcaa gatacatgta
    34801 ctctggaatg cttagataaa tgctaaaaag cggtctatcc tagctctgtc cttttacctg
    34861 agagtttgag cagttgcctg ccttgcccct tcggtgcctt tacggtctct ccagagttcc
    34921 gtccatttac agtcatggaa aatcaaacga ttccccactt ctattaaact tcattcggtg
    34981 ttggtattaa ttgattctaa ttttacaaaa aatgttggct tttgtcaatg tgtttattag
    35041 taaaaattag ttcaacagtt tttactttat aaagtccaga atactgctat cctttaaaag
    35101 tgacaatagt cgcaccactg cctccagcat tttgtggggc atagccgaaa ctcttgacat
    35161 gtttgtttct ttgcaagtat ttggtaactc cttcacggat gactcctgtt ccgataccat
    35221 ggatgatatc aacttgagcc atattgttaa gcaaagcttg gtcgatgaag gtatctagct
    35281 cattcatggc ttcttcatag cgcttgcctc gaagatccag tctagcttga ggtcctcgcc
    35341 cagaagttcg tttcacaaca ttgacctgtt tcttcttgac tggtttttct tgctgggctt
    35401 gaacaagatc aaactctttc tcttccaagg tcatcttaat caagccaact tgggcttccc
    35461 agcgaccgtc cttgagttga ctggtcaagg taccacgctg accataactg agaaccacga
    35521 tatcatctcc cacctttgga gctcgttttt tcttggcctt ttgaaggacc ttatttttag
    35581 acaagtccac tttttcagga gccaattttt tcaacttggc cttggcttca atgatttcgt
    35641 ggggcttgag ttgggattta ctgtggagat ttttgagaat ctggtcactt tcacttaggg
    35701 ccatatccac aatctcagca gcctgttcac gcgccttgtt aagctcggtt tccttttcac
    35761 gattaagctc gttgtagagt ttttttagcg cacggttcat cttgagattt tcttgctcca
    35821 cctcacggat attgtccaaa cgtttgcggc tttccagcgt ctgctcttct aattgctcaa
    35881 tgatacgatt gacgtcattg tcctgatcga tctgctgact ggcatctcct acgataactt
    35941 cagataggcc tagacgtttg gcaatttcaa aggcattact tcggccagga acaccctgca
    36001 taaagcgata ggtcgggcga agagttgcag tatcaaactc catactggca ttttgcacaa
    36061 aggctgtctc aataccgtag gccttgagtt ctggatagtg ggtcgtcgcc atggtcttga
    36121 tttgacgcag gcgaaggtcc tccagaatag ccatggcaag ggctgctccc tcttggggat
    36181 cagtaccagc ccccaactca tccaaaagta agagtgaatg ttggttgacc ttgccaagaa
    36241 tatccacgat attggtcata tggctagaga aggtagacaa gctctgctca atagactgct
    36301 catctccaat atcagcaaag atttcttcaa aaataccaac acgacttccc ttgtctgcta
    36361 aaatcggcaa tcctgactgg gccatgacct gtgtcaagcc cagagttttg agcatgatgg
    36421 tcttcccacc tgtattggga cctgtaatga caatagctgt taaatcttga ccaaaatgga
    36481 catcatttgc gacggcattt ttgaccaaag gatggcagac atggagcagt tgaatctctt
    36541 gattttctga cagctgaggc acgactgctt gtctttcttg gataaatcga accttggcac
    36601 gaatcaagtc cagatgaccg ataatccaag cgtcattagc aatctcagcc gcatgagggc
    36661 ggacacgctc agaaatttct tggagaatgc gaagcatttc atagcgctca tctgctcgca
    36721 gactagcaat ttcttcgctc agtttgacta cctcacgtgg ttcgatatag acggtgtttc
    36781 cactagcaga aatatcatga acgacacctg caatcttatt gcggtaggtg tttttgactg
    36841 gtaaaacctg acggccattt ctgctagcaa caattccttc cgtcaacatc tgcgcttttt
    36901 gcttgagcaa gtcttgtaaa acatcgcgta cctgactctc gctatcatgt atttttcgac
    36961 ggattcgcgc caattcttca ctggcaaaat tttcaatgaa acccgcatca ttaaaggcct
    37021 gaagatttcc ttgtaattgc ggaaaatcat gtaatttctc aaaccaaagg gctaattctt
    37081 ccaagctgac attttccaga ttggcataaa aactttgaag ttctcggctg gcaagaagca
    37141 cgcgtttcaa gagtaggaac tcctcgatat tgagatccgc tcccatctcc aacctcttgc
    37201 agactcctgc aatttcctta gttgagagaa tagtaaaatg cggttgctcg acgaaaagag
    37261 cctgcatttc cttcatctca gcaaaagcct gtttgatttt atctgctttg gcagtcggag
    37321 ccagttgtct caattgctcc aagccctgct cggtcaacaa atgaggctca aacaaggcct
    37381 tgaccttatc gaactctaat gtttctaata ttttcttatt catctttatt tccttgtctt
    37441 tgaatgtcta ggtgtggtag ctttttacat tctgatagat tcgagtcaaa tctgtaaaca
    37501 aagggctagg aaaactcctg ccctttttat ccgattaaat ttgtcaccca gatttgtttg
    37561 agccaactgg ttgttaccgg tatgctctgg atgatgtgtt ttgcgacgat actcttttca
    37621 agaggatttt gtataactgc catggggatg gtcgccaaga ttgtcaaggc catttgcaag
    37681 acaaataagg tcaccaacat ggacaagata cctgctgaaa cttggaacaa cttaccaccc
    37741 agttttttgc taggaatcaa gtgtaagaga agaccaagta aacgaccaat gctatagaca
    37801 atcccaaata caagcaagta gccgatacct gcataaaaga ccttatccag ctgaaagagt
    37861 tgatccgatg ggaaaaagaa agtcccctga ccttcctgcg aatttgcata agggagcaat
    37921 aaatggaatt gctctccaag ccccttataa aactggccag ccataaaagc cgatgccatg
    37981 gctgaaatca ggtaataaac ctgtaagagc aggcctctcc gatagccgat ataaaatccc
    38041 caaaccaaga ccaatagaag aaggaatgaa atcataagga atcctcaatc ttgctctgtt
    38101 cttgcttaca agtcacaagc ttgtgacgga gttcttctag ctcttgctcc ttatcgtcaa
    38161 attcaatctc acggctgagc tgagttgata aacagttgac tgccaacaaa agagcgattg
    38221 tttcatcatc tgcgctaggc atttgttctt taattgcttg gtatttttct gtcgcaacct
    38281 tagcgatttc ctccataaaa aggttgtcat gttcgcttgt caaggttaac gattttttcc
    38341 cgaatgtaaa tttgaatcga tttagatttg ccataaaatt cacctcacga tattatacca
    38401 aaattcgcta attttgtcag tttttacaaa ttttactgct tttgtggtac aatagaaact
    38461 atggcaagta tcacactcac accaagcgaa aaggatattc aggcttttct tgaacactat
    38521 caaaccagtc tggctcccag taagaatccc tatatccgct actttttgaa actacctcaa
    38581 gcaacggttt ctatctatac ttctggaaaa atcttgcttc agggtgaagg ggctgagaaa
    38641 tacgctagtt tctttggcta tcaagctgta gagcaaacca gcggacaaaa tcttccttta
    38701 attgggacag atgaggtggg aaatggttcc tactttggtg ggcttgcagt tgtggctgcc
    38761 tttgtcacat ctgaccagca cgacttttta cgaaaactcg gtgtggggga ttctaagact
    38821 ctgaccgacc aaaagatccg tcagattgct cctattctca aggaaaaaat ccagcaccag
    38881 gcactccttc tctcacccag caagtacaac gaggtcatcg gagaccgcta caatgctgtt
    38941 tcggttaagg ttgccctcca taatcaggct atctatctcc tccttcaaaa aggtgttcag
    39001 cctgagaaaa ttgtgattga tgcctttacc agtgctaaaa attatgacaa gtacttggca
    39061 caagaggcca atcgtttcag caatcctatc agcttagaag aaaaggctga gggcaaatac
    39121 ttgtctgtcg cagtttcttc tgtcattgcg cgtgatctct ttctggaaaa tcttgaaaat
    39181 ttgggacgag aactgggtta tcagctccca agtggagctg gaacggcttc tgacaaggtg
    39241 gctagccaga ttttgcaagc ctatggtatg cagggactca gcttctgcgc caaattgcac
    39301 tttaaaaaca ctgaaaaagc gaaaaaacgc ttagaaaggt aagttatgaa ttcatttaaa
    39361 aatttcttaa aagagtgggg attgttcctc ctaattctgt cattactagc tttaagtcgt
    39421 atcttttttt ggagcaatgt tcgcgtagaa ggacattcca tggatccgac cctagcggat
    39481 ggcgaaattc tcttcgttgt aaaacacctt cctattgacc gttttgatat cgtggtggcc
    39541 catgaggaag atggcaataa ggacatcgtc aagcgcgtga ttggaatgcc tggcgacacc
    39601 attcgttacg aaaatgataa actctacatc aatgacaaag aaacggacga gccttatcta
    39661 gcagactata tcaaacgctt caaggatgac aaactccaaa gcacttactc aggcaagggc
    39721 tttgaaggaa ataaaggaac tttctttaga agtatcgctc aaaaagccca agccttcaca
    39781 gttgatgtca actacaacac caactttagc tttactgttc cagaaggaga ataccttctc
    39841 ctcggagatg accgcttggt ttcgagcgac agccgccacg taggtacctt caaagcaaaa
    39901 gatatcacag gggaagctaa attccgcttc tggccaatca cccgtatcgg aacattttaa
    39961 gaaacctaag aggccgagaa tcaccaatct cagcctcttc ttctatcgtg agaaaatgat
    40021 tggtaactat ctaaacttac cagaacagaa acacctcaac tctcacctat tcatgcaaag
    40081 gaattttatg gaagtttatt tttcaggaac cattgaacgg attatttttg aaaatcccag
    40141 caatttttat cgcatcctcc tcctagaaat cgacgatacg gacgcagagg attttgatga
    40201 ttttgaaatc attgtcacag gaaccatggc tgatgtaatt gagggcgaag actatacttt
    40261 ttgggggcaa attgtccagc actccaagta tggagaacaa ctgcaaatca gtcgttatga
    40321 tcgcgcaaaa ccaactagta agggcttggt caagtacttt tcaagtagcc atttcaaggg
    40381 gattggtctc aagacagctc agaaaatcgt ggatacctat ggcgaaaata ccattgacga
    40441 aattttgcaa cacccagaaa agttagaagg catcacagga ctctctgcca aaaatcgcga
    40501 ggctttcgtc tccactctcc gtctcaacta cggaacggag atggttttgg ccaaactagc
    40561 caactacggc attcccaaca aactagcctt tcagattcaa gacttttaca aggaagaaac
    40621 ccttgatgtg gttgaaaatt atccctacca gctggttgag gatatcaagg gtttgggctt
    40681 taccattgct gaccaactag ctgaggaact aggcatcgaa agtcaggctc ctgaacgctt
    40741 ccgcgccggt ctagttcaca gtctttttca ggcctgtatg gaaacagggg acacctatgt
    40801 tgaagcacgg gatttgctgg aacaaaccct tactctcctt gagtcttccc gtcccgtgga
    40861 actagacccc agccaagttg cccaagagct ctcctacctg atcgaagaag acaaggttca
    40921 gcagattgat accaagatct ttgacaacag cctctttttc gctgaggaag gcatccgcag
    40981 tcacttggtt cgtatccttg aaaaaggaaa acagaagagt catgatttag aaaccattca
    41041 aaaacatatc actactatcg aggaagagct gggaattgag tatgataaca ttcaaaaaca
    41101 ggctatctgt gatgctatcc agaataaggt ctttatcctg acaggtgggc ctggtactgg
    41161 taagacaact gttatcaatg gaatcattgc tgtttatgcc cttttagaag gacttgactt
    41221 caggaagaaa agcaatctgc ccattcttct tgctgctcca actggtcgag ctgctcgtcg
    41281 catgaatgaa ttgacaggtt tgcctagcgc gaccatacat cgccacttgg gaatgacagg
    41341 tgacgatgat accagtcatc tggaagatta cctagatgct gagtttatca tcgtggatga
    41401 attctctatg gtagatactt ggctggccaa tcaactcttc tccaatatct cttctaacag
    41461 taagatcctc atcgtaggtg acagtgatca gctaccttct gtcagtcctg gacaggttct
    41521 agcggatctg cttcatattc ctttgattcc tcagactcgc ttggaaaaaa tttatcgaca
    41581 aagcaaagaa tcaaccatcg tcaccctagc tagtcagatt cgacagggca tcttgccagc
    41641 tgatttcacc caaaaaaaag ctgaccgttc ctactttgaa attgctagtg gccatattcc
    41701 tgccaccatt gaaaaaatct taggtgccgc cctcagaaat ggtattcccg cccgtgatat
    41761 ccaagttctg gctcctatgt accgagggac ggcagggatt gatgctatca atcagctcat
    41821 gcaagacctg ctcaatcccc cacaaaaaga tcaactcagt tttgaagctc ctcagtgtca
    41881 ctatcgtaag agagacaagg tcattcattt ggttaacgat gctgaaatca atgtctttaa
    41941 tggggattta ggagctatca cagacctgat tcctggtaaa tacaccgaat cgaaacaaga
    42001 cgagattgtc attgattttg atggtaatga agtctcttac ccccgtaacg aatggtacaa
    42061 gattcgcttg gcctatgcca tgagtattca taagtctcag ggaagtgagt tcccagttgt
    42121 catcctaccg attactagtg ctagccgacg tatgctggag cgaaatctca tctacacagc
    42181 cattacacgt gccaaaagca aactcatctt actaggcgaa ttacaagcct tcgactatgc
    42241 tacccaacac atcggaactg cccgaaaaac ctatctgatt gaacgcttca gtgatctact
    42301 ggagaatgtt gaagaaaagc aacaagctgt ctctgaaaca gtcacatcaa gtgcctctga
    42361 acaatcctac atcctaaccg aagaaaactg ggaccgcatc ccagccatga ttgggattac
    42421 agacacagac ctcaaagaga tttttggaaa atagtgcatc agaaaaccca ccaattcagg
    42481 tgggttttct gtttattaag attattttac tgttgctgtg cttgtgatca attcaacagc
    42541 ttttttgata gtgatatcat gtttcaacat gtcagctgaa agcaagtttt gaacttgtgc
    42601 aacttccatg ttgtagtctg ctgccaattg ctcaacttct ttttggattt cttcttctga
    42661 agcatcaaat ccttcagctt tggcaactgc ttcgataaca aggttagtct tagtacgtga
    42721 ctcagcttct gcttggtatt ggttgtgaag gtcttcttga gtagttccag tgatttggaa
    42781 gtacatgtca gggttgatcc cttgacgttg caaattccca aggaattcat ttactgaacg
    42841 gtgaacttct tcatggatca tttcttctgg aagttctacg atttcagcat tttctacagc
    42901 tgtatcaatt gctgcacctt caactgcatc tttgtaagct tcttctttag cagcagccaa
    42961 ttctttgcgg tatttttctt tcaagtcagc aagtgtttca acttcttcat caatgtcttt
    43021 tgcaagttca tcgtcaagag ctggaacttc tttagctttt acttcgtgga tagttgtcac
    43081 gaatttagct tctttacctg caaggtcttc tgcttggtag tcttctggga atgttacgat
    43141 aacatcaacg gtttcgccag ctgagtgacc taccaattgg tcttcgaaac cagggatgaa
    43201 ttgacctgaa ccaagtccaa gtgagaagtt ttcacctttt ccgccgtcaa attcaacacc
    43261 gtcgatagaa ccaacgaagt cgatcacaac agtgtcgccg ttttcagcag cagcttcctt
    43321 gataaccaat tcagccaggt tgttgcgttc gcgttcgata cgctcttcaa catcagcgtc
    43381 agttacttct ttttctacat caactgatac ttcaaggttt ttgtagtcac ccaattttac
    43441 ttcaggtttt gtaacgactt cagcagcgat aacccagtct tgaccttttt ccattgaagt
    43501 tacgtcaatt tttggttggg caaccacttc aagaccagct tcttttacag ctgcttcata
    43561 agcgtttggc aaaagagcgt tcataacgtc ttggtaaagt gattcttcac caaatttttt
    43621 atcgaagata ggacgtggaa ggtgaccttt acggaaacct ggaacattaa gagatttctt
    43681 cactgacttg aagacacggt ccaattctgg tttgatttgg tcttgagaga tagtgaaagt
    43741 caagacacca cggtttgttt ctttgttttc aaatgataca gacattctgt catttctcct
    43801 taaaattttt taaatacagt ctattataac attttctata aataaaatct agactaatgc
    43861 agccaattat agacaacaag cctttttc
//
LOCUS       DAVLVP010000007        38959 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.007, whole genome shotgun sequence.
ACCESSION   DAVLVP010000007 DAVLVP010000000
VERSION     DAVLVP010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.007"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            98..1123
                     /gene="trpS"
                     /locus_tag="VYN69_000363"
     CDS             98..1123
                     /gene="trpS"
                     /locus_tag="VYN69_000363"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEV1532712.1"
                     /translation="MTKPIILTGDRPTGKLHIGHYVGSLKNRVLLQEEDKYDMFVFLA
                     DQQALTDHAKDPQTIVESIGNVALDYLAVGLDPNKSTIFIQSQIPELAELSMYYMNLV
                     SLARLERNPTVKTEISQKGFGESIPTGFLVYPIAQAADITAFKANYVPVGTDQKPMIE
                     QTREIVRSFNNAYNCDVLVEPEGIYPENERAGRLPGLDGNAKMSKSLNNGIYLADDAD
                     TLRKKVMSMYTDPDHIRVEDPGKIEGNMVFHYLDVFGRPEDAQEIADMKERYQRGGLG
                     DVKTKRYLLEILERELGPIRERRIEFAKDMGEVYNMIQKGSERAREVAGQTLSEVKGA
                     MGLHYFN"
     gene            1275..2753
                     /gene="guaB"
                     /locus_tag="VYN69_000364"
     CDS             1275..2753
                     /gene="guaB"
                     /locus_tag="VYN69_000364"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEV1532713.1"
                     /translation="MSNWDTKFLKKGFTFDDVLLIPAESHVLPNDADLTTKLADNLTL
                     NIPIITAAMDTVTESQMAIAIARAGGLGVIHKNMSIAQQADEVRKVKRSENGVIIDPF
                     FLTPEHTIAEADELMGRYRISGVPVVETLENRKLVGILTNRDLRFISDYNQPISNHMT
                     SENLVTAPVGTDLATAESILQEHRIEKLPLVDEEGSLSGLITIKDIEKVIEFPNAAKD
                     EFGRLLVAGAVGVTSDTFERAEALFEAGADAIVIDTAHGHSAGVLRKIAEIRAHFPDR
                     TLIAGNIATAEGARALYEAGVDVVKVGIGPGSICTTRVIAGVGVPQVTAIYDAAAVAR
                     EYGKTIIADGGIKYSGDIVKALAAGGNAVMLGSMFAGTDEAPGETEIFQGRKFKTYRG
                     MGSIAAMKKGSSDRYFQGSVNEANKLVPEGIEGRVAYKGAAADIVFQMIGGIRSGMGY
                     CGAANLKELHDNAQFIEMSGAGLKESHPHDVQITNEAPNYSM"
     gene            complement(2804..3901)
                     /gene="recF"
                     /locus_tag="VYN69_000365"
     CDS             complement(2804..3901)
                     /gene="recF"
                     /locus_tag="VYN69_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEV1532714.1"
                     /translation="MWLQHLSLKTFRNYKETKIDFNPKLNVFLGRNAQGKTNMLEAIY
                     FLALTRSHRTQTDKNLIHFDEEQLHLSGLVQKKTGSIPLEIELTQKGRVTKVNHLKQA
                     RLSDYVGHMNVVLFAPEDLQLIKGAPSIRRKFIDMELGQIKPIYLSDLTNYNHILKQR
                     NTYLKSAQKIDETFLSVLDDQLVDYGCRVMNHRLDFIKKLESFGRKKHFELSNQIEEL
                     SISYQSSVNITDKQNLSESFKIALEKSRSRDLFKKNTGVGPHRDDISFYINGMDASFG
                     SQGQHRSLVLSIKLAEIELMESITTESPILLLDDVMSELDNTRQLKLLETISQSIQTF
                     ITTTSLDHLQNLPENLSIFTIQDGKAAVNGN"
     gene            complement(3904..4272)
                     /gene="yaaA"
                     /locus_tag="VYN69_000366"
     CDS             complement(3904..4272)
                     /gene="yaaA"
                     /locus_tag="VYN69_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEV1532715.1"
                     /translation="MEYKLFEEFITLQALLKELGITHSGGAIKSFLSEHSVYFNRELE
                     SRRGKKLRIGDKVDIPDMNIDILLTQPTSEEQDEYQADKVEKERIAKLVKKMNKGVKK
                     DKSKPASSPKSQQAPRFPGR"
     gene            4431..5681
                     /locus_tag="VYN69_000367"
     CDS             4431..5681
                     /locus_tag="VYN69_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1532716.1"
                     /translation="MELVHGISTHFIQSKKFKTNKITVRFTAPLSLDTIAGHMLSASM
                     LETANQMYPTSQDLRRHLASLYGTDMSTNCFRRGQSHIIELTFTYVRDEFLSRKNVLT
                     SQILELVKETLFSPVVVDNGFDPALFEIEKKQLLASLAADMDDSFYFAHKELDKLFFH
                     DERLQLEYSDLRNRILAETPQSSYSCFQEFLANDRIDFFFLGDFNEVEIQNVLESFGF
                     KGRKGDVKVQYCQPYSNILQEGMVRKNVGQSILELGYHYRSKYGDEQHLPMIVMNGLL
                     GGFAHSKLFTNVRENAGLAYTISSELDLFSGFLRMYAGINRENRNQARKMMNNQLLDL
                     KKGYFTEFELNQTKEMIRWSLLLSQDNQSSLIERAYQNTLFGKSSADFKSWIAKLEQI
                     DKDAICRVANNVKLQAIYFMEGIE"
     gene            5678..6961
                     /locus_tag="VYN69_000368"
     CDS             5678..6961
                     /locus_tag="VYN69_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEV1532717.1"
                     /translation="MTKVVFEEKYYPAVKEMVYRTRLANGLTVALLPKKEFKEVYGSV
                     TVQFGSIDTLVTEVDGDVKQYPGGIAHFLEHKLFEREDSSDLMSAFTSLGADSNAFTS
                     FTKTNYLFSATDYFLENLYLLDELVTSAHFTEASILTEQDIIQQEREMYQDDPDSCLF
                     FSTLANLYPGTPLATDIVGSEESISQINLTNLQENFTKFYKPVNMSLFLVGNFDVERV
                     QDYFESKELKDSDFQEVAREKLFLQPVKPTDSMRMEVSSPKLAIGVRGKREVSEADCY
                     LHHILLKLLFAMMFGWTSDRFQKCYESGKIDASLSLEVEITSRFHFVMLTMDTKEPVA
                     LSHQFRKAIRNFTKDLDITEEHLDIIKREMFGEFFSSMNSLEFIATQYDAFENGETIF
                     DLPKILQEITLEDVLDAGHRLIDDGDIVDFTIFPS"
     gene            7003..7824
                     /locus_tag="VYN69_000369"
     CDS             7003..7824
                     /locus_tag="VYN69_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1532718.1"
                     /translation="MRKKTIGEVLRLARINQGLSLDELQKKTEIQLDMLEAMEADDFD
                     QLPSPFYTRSFLKKYAWAVELDDQIVLDAYDSGSMITYEEVDVDEDELTGRRRSSKKK
                     KKKTSFLPLFYFILFALSILIFVTYYVWNYIQTQPEEPSLSNYSVVQSTSSTSSVPHS
                     SSSSSSSIESAISVSGEGNHVEIAYKTSKETVKLQLAVSDVTSWVSVSESELEGGVTL
                     SPKKKSAEATVATKSPVTITLGVVKGVDLTVDNQTVDLSKLTAQTGQITVTFTKN"
     gene            7835..8380
                     /gene="pgsA"
                     /locus_tag="VYN69_000370"
     CDS             7835..8380
                     /gene="pgsA"
                     /locus_tag="VYN69_000370"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEV1532719.1"
                     /translation="MKKEQIPNLLTIGRILFIPIFIFILTIGNSIESHIVAAIIFAVA
                     SITDYLDGYLARKWNVVSNFGKFADPMADKLLVMSAFIMLIELGMAPAWIVAVIICRE
                     LAVTGLRLLLVETGGTILAAAMPGKIKTFSQMFAIIFLLLHWTLLGQVLLYVALFFTI
                     YSGYDYFKGSAYVFKGTFGSK"
     gene            8377..9204
                     /locus_tag="VYN69_000371"
     CDS             8377..9204
                     /locus_tag="VYN69_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1532720.1"
                     /translation="MKSIIDVKNLSFRYKENQNYYDVKDITFHVKRGEWLSIVGHNGS
                     GKSTTVRLIDGLLEAESGEIVIDGQRLTEENVWNIRRQIGMVFQNPDNQFVGATVEDD
                     VAFGLENQGLSRQEMKKRVEEALALVGMLDFKKREPARLSGGQKQRVAIAGVVALRPA
                     ILILDEATSMLDPEGRRELIGTVKGIRKDYDMTVISITHDLEEVAMSDRVLVMKKGEI
                     ESTSSPRELFSRNDLDQIGLDDPFANQLKKSLSQNGYDLPENYLTESELEDKLWELL"
     gene            9189..10028
                     /locus_tag="VYN69_000372"
     CDS             9189..10028
                     /locus_tag="VYN69_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000510406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HEV1532721.1"
                     /translation="MGIALENVNFTYQEGTPLASAALSDVSLTIEDGSYTALIGHTGS
                     GKSTILQLLNGLLVPSQGSVRVFDTLITSTSKNKDIRQIRKQVGLVFQFAENQIFEET
                     VLKDVAFGPQNFGVSEEDAVKTAREKLALVGIDESLFDRSPFELSGGQMRRVAIAGIL
                     AMEPAILVLDEPTAGLDPLGRKELMTLFKKLHQSGMTIVLVTHLMDDVAEYANQVYVM
                     EKGRLVKGGKPSDVFQDVVFMEEVQLGVPKITAFCKRLADRGVSFKRLPIKIEEFKES
                     LNG"
     gene            10021..10815
                     /locus_tag="VYN69_000373"
     CDS             10021..10815
                     /locus_tag="VYN69_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1532722.1"
                     /translation="MDSMILGRYIPGDSIVHRLDPRSKLLAMMLLILIVFWANNPLTN
                     LILFIATGIFIALSGVSLSFFIQGLKSMFFLIAFTTIFQLFFISNGNVLFEFSFVRIT
                     DYALQQAGIIFCRFVLIIFFSTLLTLTTMPLSLASAVEALLAPLKRVKVPVHEIGLML
                     SMSLRFVPTLMDDTTRIMNAQKARGVDFGEGSIVQKVKAMIPILIPLFATSLKRADSL
                     AIAMEARGYQGGKGRSQYRQLKWTLKDTLTILVILVLGCCLFFLKS"
     gene            10874..11692
                     /gene="mreC"
                     /locus_tag="VYN69_000374"
     CDS             10874..11692
                     /gene="mreC"
                     /locus_tag="VYN69_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEV1532723.1"
                     /translation="MNRFKKSKYVIIVFVTVLLVSALLATTYSSTIVTKLGDGISLVD
                     RVVQKPFQWFDSVKSDLAHLTRTYNENESLKKQLYQLEVKSNEVESLKTENEQLRQLL
                     DMKSKLQATKTLAADVIMRSPVSWKQELTLDAGRSKGASENMLAIANGGLIGSVSKVE
                     ENSTIVNLLTNTENADKISVKIQHGSTTIYGIIIGYDKENDVLKISQLNSNSDISAGD
                     KVTTGGLGNFNVADIPVGEVVATTHSTDYLTREVTVKLSADTHNVDVIELVGNS"
     gene            11692..12186
                     /gene="mreD"
                     /locus_tag="VYN69_000375"
     CDS             11692..12186
                     /gene="mreD"
                     /locus_tag="VYN69_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEV1532724.1"
                     /translation="MRQLKRVGVFLLLPFFVLIDAHISQLLGSFFPHVHLASHFLFLF
                     LLFETIEVSEYLYLVYCFVIGLVYDVYFFHLIGITTLLFILLGAFLHKLNSVILLNRW
                     TRMLAMIVLTFLFEMGSYLLAFMVGLTVDSMSIFIVYSLVPTMILNFLWITVFQFIFE
                     KYYL"
     gene            12280..13458
                     /locus_tag="VYN69_000376"
     CDS             12280..13458
                     /locus_tag="VYN69_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEV1532725.1"
                     /translation="MKKKILASLLLSTVMVSQVAVLTTAHAETTDDKIAAQDNKISNL
                     TAQQQEAQKQVDQIQEQVSAIQAEQSNLQAENDRLQAESKKLEGEITELSKNIVSRNQ
                     SLEKQARSAQTNGAVTSYINTIVNSKSITEAISRVAAMSEIVSANNKMLEQQKADKKA
                     ISEKQVANNDAINTVIANQQKLADDAQALTTKQAELKAAELSLAAEKATAEGEKASLL
                     EQKAAAEAEARAAAVAEAAYKEKRASQQQSVLASANTNLTAQVQAVSESAAAPVRAKV
                     RPTYSTNASSYPIGECTWGVKTLAPWAGDYWGNGAQWATSAAAAGFRTGSTPQVGAIA
                     CWNDGGYGHVAVVTAVESTTRIQVSESNYAGNRTIGNHRGWFNPTTTSEGFVTYIYAD
                     "
     gene            13682..14461
                     /gene="rpsB"
                     /locus_tag="VYN69_000377"
     CDS             13682..14461
                     /gene="rpsB"
                     /locus_tag="VYN69_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEV1532726.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMAKYIFTERNGIHVIDLQ
                     QTVKYADQAYDFMRDAAANDAVVLFVGTKKQAADAVAEEAVRSGQYFINHRWLGGTLT
                     NWGTIQKRIARLKEIKRMEEDGTFEVLPKKEVALLNKQRARLEKFLGGIEDMPRIPDV
                     MYVVDPHKEQIAVKEAKKLGIPVVAMVDTNTDPDDIDVIIPANDDAIRAVKLITAKLA
                     DAIIEGRQGEDAVAVEAEFAASETQADSIEEIVEVVEGDNA"
     gene            14540..15580
                     /locus_tag="VYN69_000378"
     CDS             14540..15580
                     /locus_tag="VYN69_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003053075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEV1532727.1"
                     /translation="MAEITAKLVKELREKSGAGVMDAKKALVETDGDIEKAIELLREK
                     GMAKAAKKADRVAAEGLTGVYVNGNVAAVIEVNAETDFVAKNAQFVELVNTTAKVIAE
                     GKPANNEEALALIMPSGETLEAAYVSATATIGEKISFRRFALIEKTDAQHFGAYQHNG
                     GRIGVISVVEGGDEALAKQLSMHIAAMKPTVLSYKELDEQFVKDELAQLNHVIDQDNE
                     SRAMVNKPALPHLKYGSKAQLTDDVIAQAEADIKAELAAEGKPEKIWDKIIPGKMDRF
                     MLDNTKVDQAYTLLAQVYIMDDSKTVEAYLESVNASVVEFARFEVGEGIEKAANDFEA
                     EVAATMAAALNN"
     gene            15826..16149
                     /gene="tnpB"
                     /locus_tag="VYN69_000379"
     CDS             15826..16149
                     /gene="tnpB"
                     /locus_tag="VYN69_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1532728.1"
                     /translation="MMIQLSDLGQVHIVCGKTDMRQGIDSLAYVVKTHFELDSFSGQV
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGKLTWLSTEKDVKALTSEQVDWLMKGFS
                     ITPQI"
     gene            complement(16290..16598)
                     /locus_tag="VYN69_000380"
                     /pseudo
     CDS             complement(16290..16598)
                     /locus_tag="VYN69_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            16589..16987
                     /locus_tag="VYN69_000381"
     CDS             16589..16987
                     /locus_tag="VYN69_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1532729.1"
                     /translation="MKSPIPKAPLAHNFGSASIIAHTIHQKFNLKVPNYRQEEDWARM
                     GLPITRKEISNRHIKTSQYYLEPLYNLLRERLLTQSLLHADETSYRVLESDSQLTYYW
                     TFLSGKAEKQGITLYHHVLIDLFISYFNPL"
     gene            complement(17184..18104)
                     /gene="cysK"
                     /locus_tag="VYN69_000382"
     CDS             complement(17184..18104)
                     /gene="cysK"
                     /locus_tag="VYN69_000382"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEV1532730.1"
                     /translation="MSIYNNITELIGQTPIVKLNNIVPEGAADVYIKLEAFNPGSSVK
                     DRIALSMIEKAEQDGILKPGSTIVEATSGNTGIGLSWVGAAKGYKVVIVMPETMSVER
                     RKIIQAYGAELVLTPGSEGMKGAIAKAQEIAAERDGFLPLQFDNPANPEVHERTTGAE
                     ILAAFGKDGLDAFVAGVGTGGTISGVSHALKSENSNIQVFAVEADESAILSGEKPGPH
                     KIQGISAGFIPDTLDTKAYDGIVRVTSDDALALGREIGGKEGFLVGISSAAAIYGAIE
                     VAKKLGTGKKVLALAPDNGERYLSTALYEL"
     gene            complement(18389..19024)
                     /locus_tag="VYN69_000383"
     CDS             complement(18389..19024)
                     /locus_tag="VYN69_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEV1532731.1"
                     /translation="MEFRTIKEDGQVQEEIKKSRFICHAKRVYSEEEARDFITAIKKE
                     HYKATHNCSAFIIGERSEIKRTSDDGEPSGTAGVPMLGVLENHNLTNVCVVVTRYFGG
                     IKLGAGGLIRAYAGSVALAVKEIGIIEIKEQAGIAIQMSYAQYQEYSNFLKEHDLMEL
                     DTNFTDQIDTMIYVDKEEKETIKAALVEFFNGKVTLTDQGLREVEVPVNLV"
     gene            19080..20378
                     /locus_tag="VYN69_000384"
     CDS             19080..20378
                     /locus_tag="VYN69_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1532732.1"
                     /translation="MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCN
                     SIILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQEDFPKQDVLKWRGQLTPFQEKV
                     SEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
                     LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPML
                     YHAVKNSVKENGLRIFLTATSTNELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSD
                     FNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEILQEQFPNEKIGFVS
                     SVTEDRLEQVQAFRDGALTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGG
                     RVGRSMDRPTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL"
     gene            20375..21037
                     /locus_tag="VYN69_000385"
     CDS             20375..21037
                     /locus_tag="VYN69_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEV1532733.1"
                     /translation="MKCLLCGQTMKTVLTFSSLLLLRNDDSCLCSDCDSTFERIGEEN
                     CPNCMKTELSTKCQDCQLWCKEGVEVSHRAIFTYNQAMKDFFSRYKFDGDFLLRKVFA
                     SFLSEELKKYKEYQFVVIPLSPDRYANRGFNQVEGLVEAAGFEYLDLLEKREERASSS
                     KNRSERLGTELPFFIKSGVTIPKKILLIDDIYTTGATINRVKKLLEEAGAKDVKTFSL
                     VR"
     gene            21117..21665
                     /gene="raiA"
                     /locus_tag="VYN69_000386"
     CDS             21117..21665
                     /gene="raiA"
                     /locus_tag="VYN69_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000599099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEV1532734.1"
                     /translation="MIKYSIRGENLEVTEAIRDYVVSKLEKIEKYFQPEQELDARINL
                     KVYREKTAKVEVTIPLGSITLRAEDVSQDMYGSIDLVTDKIERQIRKNKTKIERKNKN
                     KVATGQLFTDALVEDSNIVQSKVVRSKQIDLKPMDLEEAILQMDLLGHDFFIYVDVED
                     QTTNVIYRREDGEIGLLEVKES"
     gene            21801..23774
                     /locus_tag="VYN69_000387"
     CDS             21801..23774
                     /locus_tag="VYN69_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEV1532735.1"
                     /translation="MKKFYVSPIFPILVGLIAFGVLSTFIIFVNNNLLTVLILFLFVG
                     GYVFLFKKLRVHYTRSDVEQIQYVNHQAEESLTALLEQMPVGVMKLNLSSGEVEWFNP
                     YAELILTKEDGDFDLEAVQTIIKASVGNPSTYAKLGEKRYAVHMDASSGVLYFVDVSR
                     EQAITDELVTSRPVIGIVSVDNYDDLEDATSESDISQINSFVANFISEFSEKYMMFSR
                     RVSMDRFYLFTDYTVLEGLMNDKFSVIDAFREESKQRQLPLTLSMGFSYGDGNHDEIG
                     KVALLNLNLAEVRGGDQVVVKENDETKNPVYFGGGSAASIKRTRTRTRAMMTAISDKI
                     RSVDQVFVVGHKNLDMDALGSAVGMQLFASNVIENSYALYDEEQMSPDIERAVSFIEK
                     EGVTKLLSVKDAMGMVTNRSLLILVDHSKTALTLSKEFYDLFTQTIVIDHHRRDQDFP
                     DNAVITYIESGASSASELVTELIQFQNSKKNRLSRMQASVLMAGMMLDTKNFTSRVTS
                     RTFDVASYLRTRGSDSIAIQEIAATDFEEYREVNELILQGCKLGSDVLIAEAKDTKCY
                     DTVVISKAADAMLAMSGIEASFVLAKNTQGFISISARSRSKLNVQRIMEELGGGGHFN
                     LAAAQIKDVTLSEAGEKLTEIVLNEIKEKEKEE"
     gene            23771..24223
                     /gene="rplI"
                     /locus_tag="VYN69_000388"
     CDS             23771..24223
                     /gene="rplI"
                     /locus_tag="VYN69_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEV1532736.1"
                     /translation="MKVIFLADVKGKGKKGEIKEVPTGYAQNFLIKKNLAKEATAQAV
                     GELRGKQKSEEKAHAEMIAEGKAIKAQLEAEETVVEFVEKVGPDGRTFGSITNKKIAE
                     ELQKQFGIKIDKRHIQVQAPIRAVGLIDVPVKIYQDITSVINLRVKEG"
     gene            24267..25619
                     /gene="dnaB"
                     /locus_tag="VYN69_000389"
     CDS             24267..25619
                     /gene="dnaB"
                     /locus_tag="VYN69_000389"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEV1532737.1"
                     /translation="MAEVEELRVQPQDILAEQSVLGAIFIDESKLVFVREYIESRDFF
                     KYAHRLIFQAMVDLSDRGDAIDATTVRTILDNQGDLQNIGGLSYLVEIVNSVPTSANA
                     EYYAKIVAEKAMLRRLIAKLTESVNQAYEASQPADEIIAQAEKGLIDVSENANRSGFK
                     NIRDVLNLNFGNLEARSQQTTDITGIATGYRDLDHMTTGLHEEELIILAARPAVGKTA
                     FALNIAQNIGTKLDKTVAIFSLEMGAESLVDRMLAAEGLVESHSIRTGQLTDEEWQKY
                     TIAQGNLANASIYIDDTPGIRITEIRSRSRKLAQETGNLGLILIDYLQLITGTGRENR
                     QQEVSEISRQLKILAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYERGGEEEEGIPNNKVEVIIEKNRSGARGTVELIFQKEYNKFSSISKREA
                     "
     gene            25621..25887
                     /locus_tag="VYN69_000390"
     CDS             25621..25887
                     /locus_tag="VYN69_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532738.1"
                     /translation="MSDAFTDVAKMKKIKEEIKAHEGQVVEMTLENGRKRQKNRLGKL
                     IEVYPSLFIVEFGNVEGDKQVNVYVESFTYSDILTEKNLIHYLD"
     gene            25976..27322
                     /gene="cbpD"
                     /locus_tag="VYN69_000391"
     CDS             25976..27322
                     /gene="cbpD"
                     /locus_tag="VYN69_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase CbpD"
                     /protein_id="HEV1532739.1"
                     /translation="MKILPFIARGTSYYLKMSVKKLVPFLVVGLMLAAGDSVYAYSGG
                     NGSIARGDDYPAYYKNGSQEIDQWRMYSRQCTSFVAFRLSNVNGFEIPAAYGNANEWG
                     HRARREGYRVDNTPTIGSITWSTAGTYGHVAWVSNVMGDQIEIEEYNYGYTESYNKRV
                     IKANTMTGFIHFKDLDGGSVGNSQSSASTGGTHYFKTKSAIKTEPLASGTVIDYYYPG
                     EKVHYDQILEKDGYKWLSYTAYNGSYRYVQLEAVNKNPLGNSVLSSTGGTHYFKTKSA
                     IKTEPLVSGTVIDYYYPGEKVHYDQILEKDGYKWLSYTAYNGSRRYIQLEGVTSSQNY
                     QNQSGNISSYGSNNSSTVGWKKINGSWYHFKSNGSKSTGWLKDGSSWYYLKLSGEMQT
                     GWLKENSSWYYLGSSGAMKTGWYQVSGKWYYSYSSGALAVNTTVDGYRVNSDGERV"
     regulatory      27405..27507
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            27578..27868
                     /locus_tag="VYN69_000392"
     CDS             27578..27868
                     /locus_tag="VYN69_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HEV1532740.1"
                     /translation="MKASIALQVLPLAQGIDRIAVIDQVIAYLQTQEVTMVVTPFETV
                     LEGEFDELMRILKEALEVAGQEADNVFANVKINVGEILSIDEKLEKYNETTH"
     gene            27831..28589
                     /locus_tag="VYN69_000393"
     CDS             27831..28589
                     /locus_tag="VYN69_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009013602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1532741.1"
                     /translation="MRNLKSIMRRHISLLGFLGVLLIWQLAGFLKLLPKFILPTPLEI
                     LQPFVRDREFLWHHSWATLRVALLGLILGVLIACLMAVLMDSLTWLNDLIYPMMVVIQ
                     TIPTIAIAPILVLWLGYGILPKIVLIILTTTFPIIVSILDGFRHCDKDMLTLFSLMRA
                     KPWQILWHFKIPVSLPYFYAGLRVSVSYAFITTVVSEWLGGFEGLGVYMIQSKKLFQY
                     DTMFAIIILVSIISLLGMKLVDISEKYVIKWKRS"
     gene            28628..29635
                     /locus_tag="VYN69_000394"
     CDS             28628..29635
                     /locus_tag="VYN69_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1532742.1"
                     /translation="MKKTWKVFLTLVTALVAVVLVACGQGTASKDNKEAELKKVDFIL
                     DWTPNTNHTGLYVAKEKGYFKEAGVDVDLKLPPEESSSDLVINGKAPFAVYFQDYMAK
                     KLEKGAGITAVAAIVEHNTSGIISRKSDNVSSPKDLVGKKYGTWNDPTELAMLKTLVE
                     SQGGDFEKVEKVPNNDSNSITPIANGVFDTAWIYYGWDGILAKSQGVDANFMYLKDYV
                     KEFDYYSPVIIANNDYLKDNKEEARKVIQAIKKGYQYAMEHPEEAADILIKNAPELKE
                     KRDFVIESQKYLSKEYASDKEKWGQFDAARWNAFYKWDKENGILKEDLTDKGFTNEFV
                     K"
     gene            29635..30363
                     /locus_tag="VYN69_000395"
     CDS             29635..30363
                     /locus_tag="VYN69_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1532743.1"
                     /translation="MTEIRLEHVSYAYGQERILEDINLQVTSGEVVSILGPSGVGKTT
                     LFNLIAGILEVQSGRIVLDGEENPKGRVSYMLQKDLLLEHKTVLGNIILPLLIQKVDK
                     AEAISRADKILATFQLTAVRDKYPHELSGGMRQRVALLRTYLFGHKLFLLDEAFSALD
                     EMTKMELHAWYLEIHKQLQLTTLIITHSIEEALNLSDRIYILKNRPGQIVSEIKLDWS
                     EDEDKEVQKIAYKRQILAELGLDK"
     gene            30482..30940
                     /locus_tag="VYN69_000396"
     CDS             30482..30940
                     /locus_tag="VYN69_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEV1532744.1"
                     /translation="MRFKNTSDHIEAYIKAILDQSGIVELQRSQLADTFQVVPSQINY
                     VIKTRFTESRGYLVESKRGGGGYIRIGRIEFSSHHEMLRDLLYSIGERVSQEIYEDIL
                     QLLVEQELMTKQEMNLLESVALDRVLGEEAPVVRANMLRQVIQEVDRKGK"
     gene            30942..33374
                     /locus_tag="VYN69_000397"
     CDS             30942..33374
                     /locus_tag="VYN69_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1532745.1"
                     /translation="MNYSKALNECIESAYMVAGHFGARYLESWHLLIAMSNHSYSVAG
                     ATLNDYPYEMDRLEEVALELTETDYSQDETFTELPFSRRLQVLFDEAEYVASVVHAKV
                     LGTEHVLYAILHDGNALATRILERAGFSYEDKKDQVKIAALRRNLEERAGWTREDLKA
                     LRQRHRTVADKQNSMANMMGMPQTPSGGLEDYTHDLTEQARSSKLEPVIGRDKEISRM
                     IQILSRKTKNNPVLVGDAGVGKTALALGLAQRIASGDVPAEMAKMRVLELDLMNVVAG
                     TRFRGDFEERMNNIIKDIEEDGQVILFIDELHTIMGSGSGIDSTLDAANILKPALARG
                     TLRTVGATTQEEYQKHIEKDAALSRRFAKVTIEEPSVADSMTILQGLKATYEKHHRVQ
                     ITDEAVETAVKMAHRYLTSRHLPDSAIDLLDEAAATVQNKAKHVKADDSDLSPADKAL
                     MDGKWKQAAQLIAKEEEVPVYKDLVTESDILTTLSRLSGIPVQKLTQTDAKKYLNLEA
                     ELHKRVIGQDQAVSSISRAIRRNQSGIRSHKRPIGSFMFLGPTGVGKTELAKALAEVL
                     FDDESALIRFDMSEYMEKFAASRLNGAPPGYVGYEEGGELTEKVRNKPYSVLLFDEVE
                     KAHPDIFNVLLQVLDDGVLTDSKGRKVDFSNTIIIMTSNLGATALRDDKTVGFGAKDI
                     RFDQENMEKRMFEELKKAYRPEFINRIDEKVVFHSLSSDHMQEVVKIMVKPLVASLAE
                     KGIDLKLQASALKLLANQGYDPEMGARPLRRTLQTEVEDKLAELLLKGDLVAGSTLKI
                     GVKAGQLKFDIA"
     gene            34016..34669
                     /gene="rr06"
                     /locus_tag="VYN69_000398"
     CDS             34016..34669
                     /gene="rr06"
                     /locus_tag="VYN69_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RR06"
                     /protein_id="HEV1532746.1"
                     /translation="MNILVADDEEMIREGIAAFLTEEGYHVIMAKDGQEVLEKFQDLP
                     IHLMVLDLMMPRKSGFEVLKEINQKHDIPVIVLSALGDETTQSQVFDLYADDHVTKPF
                     SLVLLVKRIKALIRRYYVIEDLWRYQDVTVDFTSYKAHYKNEEIDLKPKELLVLKCLI
                     QHKNQVLSREQILEEISKDVADLPCDRVVDVYIRTLRKKLALDCIVTVKNVGYKISL"
     gene            34666..35997
                     /gene="hk06"
                     /locus_tag="VYN69_000399"
     CDS             34666..35997
                     /gene="hk06"
                     /locus_tag="VYN69_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HK06"
                     /protein_id="HEV1532747.1"
                     /translation="MIKNPKLLTKSFLRSFAILGGVGLVIHIAIYLTFPFYYIQLEGE
                     KFNESARVFTEYLKTKTSDEIPSLLQSYSKSLTISAHLKRDIVDKRLPLVHDLDIKDG
                     KLSNYIVMLDMSVSTADGKQVTVQFVHGVDVYKEAKNILLLYLPYTFLVTIAFSFVFS
                     YFYTKRLLNPLFYISEVTSKMQDLDDNIRFDESRKDEVGEVGKQINGMYEHLLKVIHE
                     LESRNEQIVKLQNQKVSFVRGASHELKTPLASLRIILENMQHNIGDYKDHPKYIAKSI
                     NKIDQMSHLLEEVLESSKFQEWTECRETLTVKPVLVDILSRYQELAHSIGVTIENQLT
                     DATRVVMSLRALDKVLTNLISNAIKYSDENGRVIISEQDGYLSIKNTCAPLSDQELEH
                     LFDIFYHSQIVTDKDESSGLGLYIVSNILESYQMDYSFLPYEHGMEFKISL"
     gene            36092..36619
                     /locus_tag="VYN69_000400"
     CDS             36092..36619
                     /locus_tag="VYN69_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HEV1532748.1"
                     /translation="MVLAIILVTFLIRLIFLKRSIENEKRILSNGGKEFGVENTKRLT
                     LAHIVFYLSCLVEAMVHKTMFDGMSMVGLVLLIFSMLMLMLVIHLLGDIWTVKLMLVN
                     NHKYVDHILFRTVKHPNYFLNILPELIGLTLLSHAYMTFVLVFPVYAVILYRRIAEEE
                     KLLHEVIIPNGSIKR"
     gene            36698..>38959
                     /locus_tag="VYN69_000401"
     CDS             36698..>38959
                     /locus_tag="VYN69_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RICH domain-containing protein"
                     /protein_id="HEV1532749.1"
                     /translation="MFASKSERKVHYSIRKFSIGVASVVVASLVMGSVVHATENEGIT
                     QVATSYNKANESQTEHRKAAKQVDEDIKKMLSEIQEYIKKMLSEIQLDKRKHTQNVNL
                     NRKLSAIQTKYLYELRVLKEKSKKEELTSKTKKELDAAFEKFKKEPELTKKLAEAKQK
                     AKAQKEEDFRNYPTNTYKTLELEIAEFDVKVKEAELELVKEEAKPRNEEKIKQAKAKV
                     ESKKAEATRLEEIKTERKKAEEEAKRKAEESEKKAAEAKQKVDTKEQGKPKRRAKRGV
                     SGELATPDKKENDAKSSDSSVGEETLPSPSLNMANESQTEHRKDVDEYIKKMLSEIQL
                     DRRKHTQNVNLNIKLSAIKTKYLYELSVLKENSKKEELTSKTKAELTAAFEQFKKDTL
                     KPEKKVAEAEKKVEEAKKKAKDQKEEDRRNYPTNTYKTLELEIAESDVKVKKAELELV
                     KEEANESRNEEKIKQAKEKVESKKAEATRLEKIKTDRKKAEEEAKRKAEESEKKAAEA
                     KQKVDAEEYALEAKIAELEYEVQRLEKELKEIDESDSEDYLKEGLRAPLQSKLDTKKA
                     KLSKLEELSDKIDELDAEIAKLEVQLKDAEGNNNVEAYFKEGLEKTTAEKKAELEKAE
                     ADLKKAVDEPETPAPAPQPAPAPEKPAEKPAPAPAPEKPAPAPEKPAEKPAEKPAPTP
                     ETPKTGWKQENGMWYFYNTDGSMATGWLQNNGSWYYLNSNGAMATGWLQNNGSWYYLN
                     SNGAMATGWLQYNG"
BASE COUNT        12347 a         6330 c         8561 g        11721 t
ORIGIN      
        1 aaatctgtaa aaagcaaggc tgatttattt tgataaatta cggttatttc attaaaaaaa
       61 tgctataatt gaaaggacta tatcgaagga gaacaaaatg actaaaccca ttattttaac
      121 aggagaccgt ccaacaggaa aattgcatat tggacattat gttggaagtc tcaaaaatcg
      181 agtattatta caggaagagg ataagtatga tatgtttgtg ttcttggctg accaacaagc
      241 cttgacagat catgccaaag atcctcaaac cattgtagag tctatcggaa atgtggcttt
      301 ggattatctt gcagttggat tggatccaaa taagtcaact atttttattc aaagccagat
      361 tccagagttg gctgagttgt ctatgtatta tatgaatcta gtttcgttag cacgtttgga
      421 gcgaaatcca acagtcaaga cagagatttc tcagaaagga tttggagaaa gcattccgac
      481 aggattcttg gtctatccaa tcgctcaagc agctgatatc acagctttca aggctaatta
      541 tgttcctgtt gggacagatc agaaaccaat gattgagcaa actcgtgaaa ttgttcgttc
      601 ttttaacaat gcatataact gtgatgtctt ggtagagccg gaaggtattt atccagaaaa
      661 tgagagagca gggcgtttgc ctggtttaga tggaaatgct aaaatgtcta aatcactaaa
      721 taatggtatt tatttagctg atgatgcgga tactttgcgt aaaaaagtaa tgagtatgta
      781 tacagatcca gatcatatcc gcgttgagga tccaggtaag attgagggaa atatggtttt
      841 ccattatcta gatgtttttg gtcgtccaga agatgctcaa gaaattgctg atatgaaaga
      901 acgttatcaa cgaggtggtc ttggtgatgt gaagaccaag cgttatctac ttgaaatatt
      961 agaacgtgaa ctgggtccta ttcgtgagcg ccgtattgaa tttgctaagg atatgggaga
     1021 agtttataat atgattcaaa aaggtagtga aagagcgcgt gaagttgcgg gtcaaaccct
     1081 atctgaggta aaaggggcaa tgggacttca ttactttaac taagtttatt ttacaagaaa
     1141 ctatcttttc tatctcgaag aaaagatagt tttttattta cccctgtaac atttgacaaa
     1201 tttttataga atggtatgat agatacaata taaaaagagt caagctcaaa aagaaagaaa
     1261 agaggaaact tcgaatgtct aattgggaca ctaaattttt gaaaaaaggt tttacctttg
     1321 atgatgtatt gcttattcca gctgaaagtc atgtgttgcc taacgatgca gatttaacaa
     1381 ctaaattggc agataatttg actttaaata tcccaattat taccgctgcc atggacacag
     1441 ttacagagag tcaaatggcc attgctattg ctcgtgcagg cggtctcgga gttatccata
     1501 aaaacatgtc aattgctcaa caagcagacg aggttcgtaa ggtaaaacgt tctgaaaatg
     1561 gagttattat tgatccgttc ttcttgacgc ctgaacatac aattgctgaa gcagatgagc
     1621 ttatgggtcg ttaccgcatc agtggtgttc cagttgttga aacacttgaa aatcgtaaat
     1681 tggttggtat tttaacaaac cgagatcttc gttttatttc agattataat caaccaattt
     1741 caaaccatat gactagtgaa aatcttgtta ctgctcctgt gggtacggat cttgcaacgg
     1801 ctgagagtat tcttcaagag catcgtattg aaaaacttcc gttggtcgat gaagaaggca
     1861 gtctttctgg tttgatcact atcaaagata ttgaaaaagt tattgagttt ccaaatgcgg
     1921 ctaaagatga gtttggtcgt cttctagttg caggtgcagt aggtgttact tcagatacat
     1981 ttgaacgtgc agaggctctt tttgaggcag gagcggatgc gattgttatt gatactgcac
     2041 atggtcattc tgcaggtgtc ttgcgtaaaa ttgccgagat tcgtgctcat ttcccagatc
     2101 ggactttgat tgctggaaat attgctactg ctgaaggtgc acgtgccctt tatgaagcgg
     2161 gtgtagacgt tgttaaggtt ggtattggac caggttctat ctgtactact cgtgtgattg
     2221 ctggtgttgg tgttccgcaa gtaacagcta tctacgatgc tgcagctgtt gcgcgcgaat
     2281 atggtaaaac gattattgct gacggtggga tcaagtattc tggagatatt gtaaaagcac
     2341 ttgctgcagg tggaaatgct gttatgcttg gatctatgtt tgctggaact gatgaagctc
     2401 caggcgaaac tgaaatcttt caaggacgta aattcaagac ttaccgtggt atgggatcaa
     2461 ttgctgctat gaagaaaggt tcaagcgacc gttatttcca aggttctgtc aatgaagcaa
     2521 acaagctcgt tccagaagga attgaaggtc gtgttgctta taaaggagcg gcagctgata
     2581 ttgttttcca aatgattggt ggtattcgct ctggtatggg ttactgtggt gcagctaacc
     2641 ttaaagaact acacgataat gctcaattta ttgaaatgtc tggtgctggt ttgaaagaaa
     2701 gccatcctca tgatgtgcaa attactaatg aggcaccaaa ttattctatg taaaaaacaa
     2761 tgaaaagaac tccagtgaaa acaggagttc ttttacaatg ttgtcaattt ccatttacag
     2821 cagccttacc atcctgaata gtgaagatac ttagattttc tggcagattt tgaagatggt
     2881 ctaagcttgt tgttgtgata aaggtttgga ttgattgaga aatcgtttct aataatttta
     2941 actgtctagt gttgtcaagt tcactcatca catcgtcaag cagtaatatc ggagattctg
     3001 tggtaatgct ttccattaat tcgatttctg ctaattttat cgagaggacg agactacgat
     3061 gttgaccttg gcttccgaaa ctagcatcca tcccatttat ataaaaagaa atgtcatctc
     3121 gatgaggacc gacaccagta ttctttttaa ataaatctct ggatctactt ttttctaaag
     3181 caattttgaa agattcggat aagttttgtt tgtcagttat attgacagaa gattgatagg
     3241 atattgacaa ctcttcgatc tgattagaga gttcaaaatg tttcttacgc ccaaatgatt
     3301 ctagtttttt tatgaaatct aagcggtgat tcattacacg acatccataa tcaactagct
     3361 gatcatctaa cacagaaagg aatgtttcat ctattttttg agctgatttt aggtaagtgt
     3421 ttctttgctt taggatgtgg ttataattgg ttaagtcaga taaatagatt ggcttaattt
     3481 gcccaagttc catatcaatg aattttcgtc gaatcgaagg tgctccttta attagttgta
     3541 aatcttcagg agcaaataag acaacattca tgtgtcctac ataatctgaa aggcgtgcct
     3601 gttttaagtg attaactttt gttacacggc ctttttgtgt tagttcgatt tctaggggaa
     3661 tggatccagt ttttttctga acgagacctg aaagatgaag ttgttcctca tcaaaatgaa
     3721 tgagattttt atctgtttga gttcgatgac tacgcgttaa ggctaaaaaa tagatagcct
     3781 ctaacatgtt tgtttttcct tgtgcattac gtcccaaaaa gacattcaat ttaggattaa
     3841 agtctatttt cgtttctttg taattacgaa aagtcttgag agatagatgt tgtagccaca
     3901 tgattatcta ccagggaatc gtggagcttg ttggctttta ggtgatgaag caggtttcga
     3961 tttgtctttt ttaactccct tattcatctt tttgacaagt ttagcgatcc gttctttttc
     4021 aactttatca gcttggtatt catcttgttc ttcagaagta ggttgtgtca acaagatgtc
     4081 aatgttcatg tcagggatgt caactttatc gccaatacga agtttttttc cacgacgact
     4141 ctctaattcc ctattaaagt aaacagaatg ttcagagaga aatgatttaa tagctcctcc
     4201 gctatgtgta attccaagtt ctttgagtag tgcttggagg gtaataaatt cttcaaataa
     4261 tttgtattcc ataattcacc tcaatctttg gtattatacc atattttcct aaaaatagtg
     4321 agagagacag cgagaaatgt aagcgatttt tatttcaaaa gtgttacagg gaacaagaaa
     4381 atttcaggtt ttcgtgatat aatagaagtc tgtatataag gaggtaaatc atggagttag
     4441 tgcatggaat ttcaacacat tttatccaat caaaaaagtt taaaacaaac aaaattaccg
     4501 tgcgttttac cgctccatta tcccttgata cgattgcagg tcacatgttg agtgcaagta
     4561 tgctagagac tgctaatcag atgtacccca cttctcaaga tttgaggaga cacttggcca
     4621 gtctatacgg tacagatatg tcaaccaatt gtttcagaag agggcaaagc cacattatag
     4681 aattgacatt tacctatgtt cgtgatgagt ttttaagtag gaaaaacgtg ctaacctctc
     4741 agattttgga acttgtaaaa gaaactcttt tttcacccgt agtagttgat aatgggtttg
     4801 atccggcctt atttgaaatt gagaaaaaac aattgctagc aagtttagca gctgatatgg
     4861 atgattcttt ttattttgca cataaagaat tggataaatt gttttttcat gatgaacgtc
     4921 ttcaattgga atatagcgat ttacgaaatc gtattttagc tgaaactcca caaagttctt
     4981 attcttgttt ccaagaattt ttagccaatg atcgaataga tttctttttc ctaggtgatt
     5041 ttaatgaggt tgaaattcaa aatgtattag aatcatttgg ctttaaaggt cgaaaaggag
     5101 atgtgaaggt tcagtattgt caaccttatt ctaatatcct tcaggaaggt atggttcgga
     5161 aaaatgtggg acaatccatt ttggaattag gttatcatta ccgttctaaa tatggtgatg
     5221 agcaacattt acccatgatt gtaatgaatg gtttacttgg tggatttgct cactctaagc
     5281 tctttacaaa tgtccgtgaa aatgctggat tagcttatac catttcaagt gagcttgatt
     5341 tatttagtgg attcttgagg atgtatgctg gtatcaatcg agaaaatcgt aaccaggctc
     5401 gtaaaatgat gaataatcaa ctgcttgatt taaaaaaagg ttattttaca gagtttgagt
     5461 taaatcagac caaggaaatg attcgttggt cgttgttact ttctcaagat aatcaatctt
     5521 cattgattga acgtgcttat caaaatacct tatttggaaa atcttcagca gactttaaaa
     5581 gttggattgc aaagcttgaa caaattgaca aagatgctat ttgtagagta gctaataatg
     5641 tgaaactaca agcgatttac tttatggaag gaatagaatg acaaaggttg tttttgaaga
     5701 aaaatactat ccagctgtaa aagaaatggt ttatcgaact cgtttggcca acggattgac
     5761 agttgctctt ttgcctaaaa aggaatttaa agaggtttac gggagtgtaa ctgtacagtt
     5821 tggttcgata gatacgcttg tcacagaagt tgacggagat gtaaaacaat atcctggagg
     5881 aattgctcat tttcttgaac ataaattatt tgagagagaa gattctagtg atttgatgtc
     5941 ggcttttacg agtctaggtg cagatagtaa tgcctttaca agctttacaa aaacaaacta
     6001 tcttttttca gcaacggatt attttttaga aaatttatat ttacttgatg aattggtaac
     6061 atcagcacac tttactgaag cttccattct gacagagcag gatattattc agcaagaacg
     6121 agaaatgtac caagatgatc cagattcgtg tttattcttt tcaactttag cgaatttgta
     6181 tcctggtaca cctttagcaa ctgatatagt tggaagtgag gagtccattt cccaaatcaa
     6241 tctaactaat ttgcaagaaa attttacaaa gttttacaaa cctgtaaaca tgtctctgtt
     6301 tttagttggt aattttgatg tggagcgagt acaggactat tttgaaagca aagaactgaa
     6361 agattcagat tttcaggaag tagcaagaga aaagttgttt ttacagcctg taaagccaac
     6421 agatagtatg agaatggaag tatcttctcc caaactagcg attggagtta gaggtaagcg
     6481 agaagtttct gaagcggatt gctatctaca tcatatttta ttaaaattat tgtttgcaat
     6541 gatgtttggt tggacttcgg atcgttttca aaaatgttat gaatcaggta aaattgatgc
     6601 gtccttatct ctggaagttg aaataacaag tcgctttcat tttgtcatgt tgacaatgga
     6661 tacgaaagag ccagttgctt tgtctcatca atttaggaag gctattcgta attttacaaa
     6721 ggatttagat attacagagg aacatttaga tattatcaaa agagagatgt ttggcgaatt
     6781 tttcagtagc atgaactctc ttgaatttat tgcaacgcaa tatgatgctt ttgaaaatgg
     6841 tgagacaatt tttgatttgc cgaaaatttt acaggaaatt actttagagg atgtccttga
     6901 tgctggacat cgtttaatag atgatggtga catagttgat tttacaatat tcccatcgta
     6961 gtaacctatc ataatagaca ctagaaagaa gggatgacaa gtatgagaaa aaaaacaatt
     7021 ggagaggttt tacgattagc tagaatcaat cagggattga gtttagatga attgcagaaa
     7081 aagacagaaa tccagttaga tatgttggaa gcaatggaag cagacgattt cgatcaactt
     7141 ccaagtcctt tttacacgcg ttctttcttg aaaaaatatg catgggctgt tgagttagat
     7201 gaccaaattg ttttggatgc ttatgattct gggagtatga ttacttatga ggaagtagat
     7261 gttgatgaag atgagttgac aggtcgtaga cgttcaagta agaaaaagaa gaaaaaaaca
     7321 tcatttttac ctttatttta ttttatcctt tttgctttat cgattttaat ttttgtgact
     7381 tattatgttt ggaactatat tcaaactcaa ccagaggagc cttctctttc taattacagt
     7441 gtggttcaat caacaagttc aactagctct gttccccact cctcaagtag tagttcttct
     7501 agtatagaat cagctataag tgtatcaggc gaaggaaatc atgtagaaat cgcttataag
     7561 acaagtaagg aaacagttaa attgcaattg gcagtttcag atgttacaag ttgggtcagt
     7621 gtttcagaaa gcgaacttga gggcggtgta accttatcgc caaagaagaa aagtgcagaa
     7681 gcaacagttg caactaaaag tcctgtaaca attacgttag gtgttgtaaa aggtgttgat
     7741 ttgacagtag ataatcagac tgttgattta tcgaaattaa cagctcagac tggacaaatc
     7801 actgtaacct ttactaaaaa ttaaggaaaa acgaatgaaa aaagaacaaa ttcccaatct
     7861 cttaacaata ggtcgaattc tctttatacc tatttttatc tttattttaa cgataggaaa
     7921 ttcgatagag agtcatatag ttgcagctat tatctttgct gttgccagta ttaccgacta
     7981 tttagatgga tatttagctc gtaaatggaa tgtggtcagt aattttggta aatttgcaga
     8041 tcctatggcg gataagttac tagttatgtc ggcttttatt atgttgattg agttaggtat
     8101 ggctccggct tggattgttg cagtgattat ctgtcgtgag ttagctgtga caggtttaag
     8161 gcttttattg gttgaaactg gtggaacaat tttagcagca gcaatgcctg gaaaaattaa
     8221 aacttttagt cagatgtttg ctattatttt cttgctatta cattggactt tgcttggtca
     8281 agttctactt tatgtagcct tatttttcac tatctactct ggctatgact atttcaaggg
     8341 tagtgcctat gtatttaaag ggacatttgg ttcgaaatga aatcaataat tgatgtaaaa
     8401 aatctttctt ttcgctataa agaaaatcag aactactacg atgtgaagga tattacgttt
     8461 cacgtgaaac gtggagaatg gctttcgatt gtagggcata atggtagtgg taaatcaacg
     8521 acggttcgat taattgatgg cttactggaa gcagaatctg gagagattgt aattgatggc
     8581 caacgtttga ctgaggaaaa tgtttggaat atacgtcgtc aaatcggtat ggtttttcaa
     8641 aatccagaca atcaatttgt tggagcgact gttgaagatg atgttgcttt tggtttggaa
     8701 aatcagggac tttctcgtca agaaatgaaa aagagagtgg aagaagctct ggctttagtt
     8761 ggcatgttgg actttaaaaa gagagagcca gcgcgtctat caggtggcca aaagcaacgt
     8821 gtggccattg caggtgttgt agccctaaga ccagctattt taatcttaga tgaagcaacg
     8881 agtatgttgg atcctgaggg gcgtagagaa cttattggga cagtaaaagg aattcgaaaa
     8941 gactatgata tgacggtcat ttctattacc catgatttgg aagaagtcgc catgagtgat
     9001 cgcgtattgg tcatgaaaaa aggggaaatt gaatcaacta gtagtccaag ggagcttttc
     9061 tctcgaaatg atttagatca aattggatta gacgatcctt ttgctaatca attaaaaaaa
     9121 tctttgagcc agaatggcta tgatttacct gaaaattatt tgacagaaag tgagctagag
     9181 gataagctat gggaattgct ctagaaaatg tgaattttac atatcaagaa ggtactccct
     9241 tagcttcagc agctttgtcg gatgtttctt tgacgattga agatggctct tatacagctt
     9301 taattgggca cacaggtagt ggtaaatcaa ctattttaca actcttaaat ggtttattgg
     9361 tgccaagtca agggagtgtg agggtttttg ataccttaat cacctcgact tctaaaaata
     9421 aagatattcg tcaaattaga aaacaggttg gcttggtatt tcagtttgct gaaaatcaga
     9481 tttttgaaga aacggttttg aaggacgttg cttttggacc gcaaaatttt ggagtttctg
     9541 aagaagatgc tgtgaagact gcgcgtgaga aactggctct ggttggaatt gatgaatcac
     9601 tttttgatcg tagtccgttt gagctgtcag ggggacaaat gagacgtgtt gccattgcag
     9661 gcatacttgc catggagcca gctatattag tcttagatga gccaacagct ggtctagatc
     9721 ctctagggag aaaagagttg atgaccctgt tcaaaaaact ccaccagtca gggatgacca
     9781 tcgtcttggt aacgcatttg atggatgatg ttgctgaata tgcgaatcaa gtctatgtaa
     9841 tggaaaaggg acgtttagta aaggggggca aaccaagtga tgtctttcaa gacgttgttt
     9901 ttatggaaga agttcagttg ggagtaccta aaattacggc cttttgtaaa cgattggctg
     9961 atagaggcgt gtcatttaaa cgattaccga ttaagataga ggagttcaag gagtcgctaa
    10021 atggatagta tgattttggg gcgttatatc ccaggggatt cgattgttca ccgattggat
    10081 ccacgtagca aattgcttgc tatgatgcta ctgattttga ttgtgttttg ggctaataac
    10141 cccttgacga atctgattct ttttatagcg acagggatat ttattgcctt gtcaggagta
    10201 tctctttcat tttttattca gggcttgaag tctatgtttt tcttgattgc cttcacaact
    10261 atttttcaac tatttttcat ttctaatggg aatgttttat ttgagttttc gtttgtgaga
    10321 atcacggatt atgctttgca acaagctggg attatttttt gtcgctttgt attgattatt
    10381 ttcttttcaa ctttgttaac cttaacaacc atgcctttaa gtttggcatc agctgtcgaa
    10441 gctttattag cacctttaaa gcgtgtgaaa gttccagttc atgaaattgg attgatgctg
    10501 tctatgagtt tgcgttttgt cccaaccttg atggatgata cgactcggat tatgaatgca
    10561 cagaaagctc gtggagtgga ttttggagaa ggaagcatcg ttcaaaaagt aaaggcgatg
    10621 attcccattt tgattcctct ttttgcgaca agtttaaaac gtgcagattc cttggctatc
    10681 gctatggaag cgcgtggcta tcagggtgga aaaggcagaa gtcaataccg acaattgaaa
    10741 tggactctaa aggatacgct gaccattctt gttattctcg tacttggttg ttgtttattt
    10801 ttcttaaaat cttagtagct ttcaggaatt gataaaaagt tactgtaaca gtttttgata
    10861 taaaggtagg aatatgaacc gttttaaaaa atcaaaatat gtcattattg tttttgtcac
    10921 tgttttgctt gtgtcagctc tcttagcgac gacttattca agtacaattg tgacaaaatt
    10981 aggagatgga atctcattgg ttgatagagt tgtacaaaaa ccttttcagt ggtttgattc
    11041 tgtcaaatca gatttggctc atttgacacg aacatataat gaaaatgaaa gtttgaagaa
    11101 acagctttac caattagaag ttaaatcaaa tgaggtggaa agtttaaaga cagaaaatga
    11161 acaactgcgc caattgcttg atatgaagtc taaattgcaa gccacaaaga ctttagcagc
    11221 agatgttatt atgcgttctc cggtatcttg gaagcaggag ttgaccttag atgcaggtag
    11281 atcaaaaggt gcttctgaga acatgttagc tattgcaaat ggtggcttga ttgggagtgt
    11341 ttcaaaagta gaggagaact ctactatagt caaccttctg acaaatacgg aaaatgctga
    11401 taagatttct gttaaaattc aacatggctc tactacaatt tatggaatta ttattggcta
    11461 tgacaaggaa aatgacgttc ttaaaattag ccaattaaat agtaatagcg atattagtgc
    11521 gggagataag gtgactactg gtggattagg aaactttaac gttgctgata ttcctgttgg
    11581 tgaagtggtt gccacaacgc atagtacaga ctatttgaca cgagaagtaa ctgttaaatt
    11641 gagtgcagat actcataatg tagatgtgat agaattagtg gggaattcat aatgagacag
    11701 ttgaagcgag ttggagtatt tttattgctt cctttctttg ttctaattga cgcccatatt
    11761 agccagcttc tgggctcatt tttcccccat gtacatttgg ctagtcattt tctttttcta
    11821 tttctcttat ttgagacgat agaagtatca gagtatctct acctagtcta ttgttttgtt
    11881 ataggcttgg tttatgatgt ttactttttc catctaatag ggattacaac tctcttattt
    11941 atcttattgg gagccttcct tcataaattg aatagtgtta ttttgttgaa tcgttggaca
    12001 agaatgctag ctatgattgt gctgacattc ctgtttgaaa tgggtagtta tcttttggct
    12061 tttatggtag ggttgacagt agatagcatg tcgattttta tagtctatag cttggtaccg
    12121 acgatgattt taaatttttt atggattact gtttttcaat ttatttttga aaaatattat
    12181 ctataagaac gacatataaa tgtaacaaag gcgtaatatt tattaggcct ttttttggta
    12241 tactagtatt gtctttaaaa gaaggagtat ctacgtaata tgaagaaaaa aatcttagcg
    12301 tcacttttat taagtacagt aatggtttct caagtagctg ttttaacaac tgcgcatgca
    12361 gaaacgactg atgacaaaat tgctgctcaa gataataaaa ttagtaactt aacagcacaa
    12421 caacaagaag cccaaaaaca agttgaccaa attcaggagc aagtatcagc tattcaagct
    12481 gagcagtcta acttgcaagc tgaaaatgat agattacaag cagaatctaa gaaactcgag
    12541 ggtgagatta cagaactttc taaaaacatt gtttctcgta accaatcgtt ggaaaaacaa
    12601 gctcgtagtg ctcaaacaaa tggagccgta actagctata tcaataccat tgtaaactca
    12661 aaatcaatta cagaagctat ttcacgtgtt gctgcaatga gtgaaatcgt atctgcaaac
    12721 aacaaaatgt tagaacaaca aaaggcagat aaaaaagcta tttctgaaaa acaagtagca
    12781 aataatgatg ctatcaatac tgtaattgct aatcaacaaa aattggctga tgatgctcaa
    12841 gcattgacta cgaaacaggc agaactaaaa gctgctgaat taagtcttgc tgctgagaaa
    12901 gcgacagctg aaggggaaaa agcaagtcta ttagagcaaa aagcagcagc tgaggcagag
    12961 gctcgtgcag ctgcggtagc agaagcagct tataaagaaa aacgagctag ccaacaacaa
    13021 tcagtacttg cttcagcaaa cactaactta acagctcaag tgcaagcagt atctgaatct
    13081 gcagcagcac ctgtccgtgc aaaagttcgt ccaacataca gtacaaacgc ttcaagttat
    13141 ccaattggag aatgtacatg gggagtaaaa acattggcac cttgggctgg agactactgg
    13201 ggtaatggag cacagtgggc tacaagtgca gcagcagcag gtttccgtac aggttcaaca
    13261 cctcaagttg gagcaattgc atgttggaat gatggtggat atggtcacgt agcggttgtt
    13321 acagctgttg aatcaacaac acgtatccaa gtatcagaat caaattatgc aggtaatcgt
    13381 acaattggaa atcaccgtgg atggttcaat ccaacaacaa cttctgaagg ttttgttaca
    13441 tatatttatg cagattaatt tacagaggga ctcgaataga gccctctttt caggttttac
    13501 cgtgacaatc cctattaaaa attatatcaa aatagcttga aaatattgga aaagtatggt
    13561 agaatgaaaa ttgtcgtgtg aacgataata ctcattcttg atgaattgtg aagcagttgc
    13621 ccttgggtcg ttttgcgagt tgaagtcaag aagaggaaaa aaacaaaaag gagaaatact
    13681 catggcagta atttcaatga aacaacttct tgaggctggt gtacactttg gtcaccaaac
    13741 tcgtcgctgg aatcctaaga tggctaagta catctttact gaacgtaacg gaatccacgt
    13801 tatcgacttg caacaaactg taaaatacgc tgaccaagca tacgacttca tgcgtgatgc
    13861 agcagctaac gatgcagttg tattgttcgt tggtactaag aaacaagcag ctgatgcagt
    13921 tgctgaagaa gcagtacgtt caggtcaata cttcatcaac caccgttggt tgggtggaac
    13981 tcttacaaac tggggaacaa tccaaaaacg tatcgctcgt ttgaaagaaa ttaaacgtat
    14041 ggaagaagat ggaactttcg aagttcttcc taagaaagaa gttgcacttc ttaacaaaca
    14101 acgtgcgcgt cttgaaaaat tcttgggcgg tatcgaagat atgcctcgta tcccagatgt
    14161 gatgtacgta gttgacccac ataaagagca aatcgctgtt aaagaagcta aaaaattggg
    14221 aatcccagtt gtagcgatgg ttgacaccaa tactgatcca gatgatatcg atgtaatcat
    14281 cccagctaac gatgacgcta tccgtgctgt taaattgatc acagctaaat tggctgacgc
    14341 tattatcgaa ggacgtcaag gtgaggatgc agtagcagtt gaagcagaat ttgcagcttc
    14401 agaaactcaa gcagattcaa ttgaagaaat cgttgaagtt gtagaaggcg acaacgctta
    14461 atttatacaa atagtaatta cctaggaggg cggggcttag cccggctctc ctattttcaa
    14521 aaaatatagg agaattaaaa tggcagaaat tacagctaaa cttgtaaaag agttgcgtga
    14581 aaaatctggt gccggtgtta tggacgctaa aaaagcgctt gtagaaacag acggtgacat
    14641 cgaaaaagcg attgaattgc ttcgtgaaaa aggtatggct aaggcagcta agaaagctga
    14701 ccgtgttgct gcagaaggtt tgactggtgt ttatgttaac ggtaatgttg cagcagttat
    14761 tgaagtaaac gctgaaactg acttcgttgc aaaaaacgct caattcgttg aattggtaaa
    14821 tactacagct aaagtcattg ctgaaggaaa acctgctaac aatgaagaag ctcttgcttt
    14881 gataatgcct tcaggtgaaa ctcttgaagc tgcatacgta tctgcaacag caactatcgg
    14941 agagaaaatc tcattccgtc gctttgcatt gattgaaaaa acagacgcac aacactttgg
    15001 agcataccaa cataacggtg gacgtatcgg tgttatttca gttgttgaag gtggagacga
    15061 agcacttgct aaacaattgt caatgcacat cgcagcgatg aaaccaacag ttctttctta
    15121 caaagaattg gatgagcaat tcgttaaaga tgagttggca caattgaatc acgttatcga
    15181 ccaagacaac gaaagccgtg caatggttaa taaaccagct cttccacact tgaagtatgg
    15241 atcaaaagct caattaactg atgatgttat tgctcaagct gaagctgaca tcaaagctga
    15301 attggctgca gaaggcaaac cagaaaaaat ctgggacaaa attattccag gtaaaatgga
    15361 tcgcttcatg cttgataaca ctaaagttga ccaagcttac acacttcttg cacaagttta
    15421 catcatggat gacagcaaga cagttgaagc ataccttgaa tcagttaacg cttcggtagt
    15481 tgagtttgct cgctttgaag ttggtgaagg tatcgagaaa gctgcaaacg acttcgaagc
    15541 tgaagttgca gctacaatgg cagcagcctt gaataactaa taatagaaag gaagcaattt
    15601 gcttcctttt ttgtataggg cttatatttt tcctgaaatt gtttttcccc cagtctcact
    15661 cgattagaat aattaaaaca aaaagcccta taataagggc taagtgcatt taggagacta
    15721 tacttcaaat tcatagtaag cgcgtcataa caaggtatct atcctaattc cccaacttcc
    15781 agtatactag ataaaaaata attatcggag gaaaggtatt gtactatgat gattcaacta
    15841 agtgatttag gtcaagttca cattgtttgt ggcaagacag atatgaggca gggaatagac
    15901 tcattagcct atgtagttaa aacccacttt gaattggatt ctttctccgg tcaagtcttt
    15961 ctcttttgtg gtggacgtaa agaccgcttt aaagtccttt actgggatgg tcaaggattt
    16021 tggctactat ataaacgctt tgagaacgga aaactgactt ggctaagtac agaaaaggat
    16081 gtcaaagctc tcacatctga acaagtagac tggcttatga agggcttttc tatcactccc
    16141 caaatatagt ggtttgaaac tagaatagta cacctctgct tctaaaacat tgttagaaat
    16201 cgatttgact gtcctgttct tatttcattt tactataaat tcatcagaaa gtcgtgattt
    16261 ctattgaaat gaggactttc tttttatact catctgtttt cacaaagcat tctagtccat
    16321 ctccgattaa cgatggactt tatcacctcc ttctccagtc cttgtataac atcttggagt
    16381 tgattcatga catcttccaa aatttgaaag gctttattct taaatccacg tttacgaatc
    16441 tctttccaca cttgttcaat ggggttcatc tctggtgtgt atggaggaat aaatgcaaag
    16501 ccaatattag tcggaatctt taaggtactt gatttatgcc atatagcatt gtccataacg
    16561 agtaaaagat aatcatctgg ataagcttgt gaaatctcct attcctaaag cccctttagc
    16621 gcataacttt ggctcagctt ctattatcgc tcacaccatc catcagaagt ttaatctgaa
    16681 ggtacccaat tatcgccaag aagaagattg ggctaggatg ggtttaccaa tcacacgtaa
    16741 ggaaatctct aatcggcata tcaagacgag tcaatactat ttggagcccc tttataacct
    16801 cttgcgagag agactattga ctcagtcctt acttcatgcg gatgaaactt cttatagggt
    16861 gctagagagt gatagtcagc tgacttacta ttggactttt ttgtctggga aagctgagaa
    16921 acaagggatt acgctttacc atcatgtcct aatcgatttg ttcatatctt atttcaatcc
    16981 attataaata gtgagaaata tctatcctat cttctagaat gtcttccaaa cgtggaaact
    17041 ctcgtaaaca aagaggtttt agaggcctat ttaccatgga ctaaagttgt acaagaaaag
    17101 tgcaaataag aaatctccag attaggaact atccgtgagt tctctagtct ggagattttt
    17161 caatagactt cgttattgga cggttacaat tcataaagtg ctgtagagag ataacgttca
    17221 ccgttatctg gtgctagagc aaggactttt ttacctgtac ctaatttttt ggcaacctcg
    17281 atggctccgt agatagctgc agctgaggaa atccctacaa ggaagccttc ttttccacca
    17341 atttcacgtc cgagtgcaag agcgtcatct gatgttacac gaacgatacc atcataggct
    17401 ttagtatcaa gtgtatcagg aataaatcca gctgagatac cttgaatttt gtgaggaccg
    17461 ggtttttcac cagatagaat agcagattca tctgcttcta ctgcaaaaac ttgaatgtta
    17521 gaattttctg atttgagtgc atgagaaaca ccagaaatcg ttccaccggt acctactcca
    17581 gcaacaaagg catctaatcc atctttaccg aaagcagcta gtatctcagc tcctgttgtt
    17641 ctttcgtgta cttctggatt agctggattg tcaaattgaa gaggaaggaa accatcacgt
    17701 tcagcagcga tttcttgagc cttagcaata gcacctttca ttccctcgct accaggagtt
    17761 aggacgagtt cagcaccata agcttggata attttacgtc gttctacact catagtttca
    17821 ggcataacga tgacgacttt atacccttta gcagcaccta cccatgaaag tccaatacca
    17881 gtgtttccac ttgttgcttc aacaatagta gaaccaggtt tcagaatacc atcttgttca
    17941 gctttttcaa tcatgctaag ggcaatacgg tcttttacag atgaaccagg attaaatgct
    18001 tcaagcttta tatagacgtc tgcagcacct tctggcacga tgttgttaag tttaacaatt
    18061 ggtgtttgac cgattaattc agtaatgttg ttataaatag acatatgaat acctctttgt
    18121 ataagatatc tttagtataa cgctatctat accatttgta aaatatagaa atcctatcga
    18181 agcgatagga tttctatata tcatagatca aagtcattaa ttttcaagtg attgcgttca
    18241 gctaattcta gtaaataggt gtgaaggaaa acgatatcag gtttcgtttg aattgcttca
    18301 ttttctagta tattttggta cgcatctttt tattttgctc gctaaagtag ttgtgagaat
    18361 gtcaacgaaa cgttgtatta ttcattgttt acactaagtt tacaggaact tcaacctctc
    18421 gtaaaccttg gtcagttaaa gtgacttttc cattaaaaaa ctccacaagt gcagctttaa
    18481 tagtttcttt ttcttcttta tcaacataaa tcatcgtatc gatttgatct gtaaagtttg
    18541 tatccagctc catgagatca tgttctttaa ggaagttact gtactcttgg tactgagcat
    18601 aagacatttg aatagcaatg ccagcctgtt cttttatttc aataatacca atttctttga
    18661 cagctaaggc gacactgccg gcgtaagcac gaattagtcc tccagcgcct agtttaatac
    18721 caccaaagta gcgtgtcacg accacacaga cattggtgag attgtgattt tctagtaccc
    18781 caagcatggg aacaccagca gtaccactag gttcaccatc atcacttgta cgtttaattt
    18841 cactacgttc tccaataata aaggcagagc agttatgtgt cgctttgtag tgttcttttt
    18901 tgatggcagt aatgaagtca cgagcctctt cttcgctata aacacgcttg gcatggcaga
    18961 taaagcgaga ttttttgatt tcttcttgga cttgaccgtc ctctttaatt gttctaaatt
    19021 ccatgattta attgtactaa aaaatcatct aaagtgctag tttttacgaa taaagaagta
    19081 tgaaagtaaa tttagattat ctcggtcgtt tatttactga gaatgaatta acagaagaag
    19141 aacgtcagtt ggcggagaaa cttccagcaa tgagaaagga gaaggggaaa cttttctgtc
    19201 aacgctgtaa tagtattatt ctagaagaat ggtatttgcc catcggtgct tactattgtc
    19261 gagagtgctt gctgatgaag cgagtcagaa gtgatcaaac tttatactat tttccgcagg
    19321 aggattttcc gaagcaagat gttctcaaat ggcgcggcca attaactcct tttcaagaga
    19381 aggtgtcaga gggactgctt caagcagtag acaagcaaaa gccaacctta gttcatgcgg
    19441 taacaggagc tggaaagaca gaaatgattt atcaagtagt ggctaaagtg atcaatgcgg
    19501 gtggtgcagt gtgtttggct agtcctcgca tagatgtttg tttggagctg tacaagcgcc
    19561 tgcaacagga tttttcttgc ggaatagctt tgctacatgg agaatcggaa ccttattttc
    19621 gaacaccact agttgttgca acaacccatc agttattgaa gttttatcaa gcttttgatt
    19681 tgctgatagt ggatgaagta gatgcttttc cttatgttga taatcccatg ctttaccacg
    19741 ctgtcaagaa tagtgtaaag gagaatggat tgagaatctt tttaacagcg acttcgacca
    19801 atgagttaga taaaaaggtc cgtttaggag aactaaaaag actgaattta ccgagacggt
    19861 ttcatggaaa tccgttgatt attccaaaac caatttggtt atcggatttt aatcgctact
    19921 tagacaagaa tcgtttgtca ccaaagttaa agtcctatat tgagaagcag agaaagacag
    19981 cttatccgtt actcattttt gcttcagaaa ttaagaaagg ggagcagtta gcagaaatct
    20041 tacaggagca atttccaaat gagaaaattg gctttgtatc ttctgtaaca gaggatcgat
    20101 tagagcaagt acaagctttt cgagatggag cactgacaat acttatcagt acgacaatct
    20161 tggagcgtgg agttaccttc ccttgtgtgg atgttttcgt agtagaggcc aatcatcgtt
    20221 tgtttaccaa gtctagtttg attcagattg gtggacgagt tggacgaagc atggatagac
    20281 cgacaggaga tttgcttttc ttccatgatg ggttaaatgc ttcaatcaag aaggcgatta
    20341 aggaaattca gatgatgaat aaggaggctg gtctatgaag tgcttgttat gtgggcagac
    20401 tatgaagact gttttaactt ttagtagtct cttacttctg aggaatgatg actcttgtct
    20461 ttgttcagac tgtgattcta cttttgaaag aattggggaa gagaactgtc caaattgtat
    20521 gaaaacagag ttgtcaacaa agtgtcaaga ttgtcaactt tggtgtaaag agggagttga
    20581 agtcagtcat agagcgattt ttacttacaa tcaagctatg aaggattttt tcagtcggta
    20641 taagtttgat ggagacttcc tgttaagaaa agttttcgct tcatttttaa gtgaggagtt
    20701 gaaaaagtac aaagagtatc aatttgttgt aattccccta agtcctgata gatatgctaa
    20761 tagaggattt aatcaggttg agggcttggt agaggcagca ggctttgagt atctggattt
    20821 attagagaaa agagaagaga gagccagttc ttctaaaaat cgttcagagc gcttggggac
    20881 agaacttcct ttctttatta aaagtggagt cactattcct aaaaaaatcc tacttataga
    20941 tgatatctat actacaggag caactataaa tcgtgttaag aaactgttgg aagaagctgg
    21001 tgctaaggat gtaaaaacat tttcccttgt aagatgagga aaaaatttgc aaaaatagaa
    21061 tgaaagcgtt ataataaaat cataaaaaca aaaatgttta gaaagaaggt actcatatga
    21121 ttaaatatag tatccgtggt gaaaacctag aagtaacaga agcaattcgt gattatgtag
    21181 tttctaaact cgaaaagatc gaaaagtact tccaaccaga acaagagttg gatgcccgaa
    21241 ttaacttaaa agtttatcgt gaaaaaacgg ctaaagtgga agtaacgatt ccgcttggat
    21301 ctattactct ccgcgcagaa gatgtatctc aagatatgta tggttcaatt gaccttgtaa
    21361 ctgataaaat tgaacgtcag attcgtaaaa ataaaacaaa aatcgagcgt aaaaataaaa
    21421 ataaggtagc aactggtcaa ttatttacag atgctttggt ggaagattca aatattgtcc
    21481 agtctaaagt tgttcgttca aaacaaattg atttaaaacc aatggatttg gaagaagcaa
    21541 ttctacaaat ggatttattg gggcatgatt tctttatcta tgtggatgtt gaagatcaga
    21601 caaccaatgt gatttatcgt cgtgaggatg gcgaaattgg tttgttagag gttaaagaat
    21661 cttaatttca atatgtgtaa aggtaggttt actgaattgt aaactacctt tttcttataa
    21721 ttgatagaat tactgattac acttttaaaa agtcgctttt tgaggggtat tatggtataa
    21781 tgggtgccaa gaggttttga atgaaaaaat tttatgtaag tccaattttt cctattctag
    21841 taggattgat tgcgtttgga gtcttatcca ctttcattat ttttgttaat aataatctgt
    21901 tgacggtttt aattttgttt ctttttgtag gaggctatgt ttttttattt aagaaactga
    21961 gagtgcatta tacaaggagt gatgtagaac agatacagta tgtaaaccac caagcggaag
    22021 aaagtttgac agctctattg gaacagatgc ctgtaggtgt tatgaaattg aatttatctt
    22081 ctggagaggt tgagtggttt aatccctatg ctgaattgat tttgaccaag gaagatggtg
    22141 attttgattt agaagctgtt caaacgatta ttaaggcttc agtaggaaat ccgtctactt
    22201 atgccaagct tggtgagaag cgttatgctg ttcatatgga tgcttcttcc ggtgttttgt
    22261 attttgtaga tgtatccagg gaacaagcca taacagatga attggtaaca agtcgaccag
    22321 tgattgggat tgtctctgtg gataattatg atgatttgga ggatgcaact tctgagtcag
    22381 atattagtca aatcaatagt tttgtagcta attttatatc agagttttca gaaaaataca
    22441 tgatgttttc tcgtcgggta agtatggatc gattttatct atttactgac tacacggtgc
    22501 ttgagggctt gatgaatgat aaattttctg ttattgatgc tttcagagaa gagtcgaaac
    22561 agagacagtt gcccttgacc ttaagtatgg gattttctta tggcgatgga aatcatgatg
    22621 agatagggaa agttgctttg ctcaatttga acttggctga agtacgtggt ggcgaccagg
    22681 tggttgttaa ggaaaacgac gaaacgaaaa atccagttta ttttggtggt gggtctgctg
    22741 cttcaatcaa gcgtacacgg actcgtacgc gcgctatgat gacagctatt tcagataaga
    22801 ttcggagtgt agatcaggtt tttgtagtcg gtcacaaaaa tttagacatg gatgctttgg
    22861 gctctgctgt aggtatgcag ttgttcgcca gcaatgtgat tgaaaatagc tatgctcttt
    22921 atgatgaaga acaaatgtct ccagatattg aacgagctgt ttcattcata gaaaaagaag
    22981 gagttacgaa gttgttgtct gttaaggatg caatggggat ggtgaccaat cgttctttgt
    23041 tgattcttgt agaccattca aagacagcct taacattatc aaaagaattt tatgatttat
    23101 ttacccaaac cattgttatt gaccaccata gaagggatca ggattttcca gataatgcag
    23161 tcattactta tatcgaaagt ggtgcaagta gtgccagtga gttggtaacg gaattgattc
    23221 agttccagaa ttctaagaaa aatcgtttga gtcgtatgca agcaagtgtt ttgatggctg
    23281 gtatgatgtt ggatactaaa aatttcacct cgcgagtaac tagtcggaca tttgatgttg
    23341 ctagctatct cagaacgcgc ggaagtgata gtattgctat ccaggaaatt gctgcgacag
    23401 attttgaaga atatcgtgag gttaatgaac ttattttaca ggggtgtaaa ttaggttcag
    23461 atgtactaat agcagaggct aaggacacga aatgctatga tacagttgtt attagtaagg
    23521 cagcagatgc catgttagcc atgtcaggta ttgaagcgag ttttgttctt gcgaagaata
    23581 cacaaggatt tatctctatc tcagctcgaa gtcgtagtaa actgaatgta caacggatta
    23641 tggaagagtt aggcggtgga ggccacttta atttggcagc agctcaaatt aaagatgtaa
    23701 ccttgtcaga agcaggtgaa aaactgacag aaattgtatt aaatgaaata aaggaaaagg
    23761 agaaagaaga atgaaagtaa tctttttagc agatgttaaa ggaaaaggta aaaaaggcga
    23821 aattaaggaa gtaccaacag ggtatgcgca aaactttctt atcaaaaaga atctagccaa
    23881 agaagcgact gctcaagctg taggtgaact tcgtggtaaa caaaaatcgg aagaaaaagc
    23941 tcacgctgag atgattgcag aaggaaaagc aattaaagca caacttgaag cagaagaaac
    24001 tgttgtagaa tttgttgaaa aagttggtcc agatggtcgt acctttggtt ctattaccaa
    24061 taagaagatt gcagaagaat tgcaaaagca atttggaatt aagattgata aacgtcatat
    24121 tcaagtacaa gctccgattc gagcggttgg tttgattgat gtgccagtga aaatatatca
    24181 agatatcaca agtgtaatca atcttcgtgt gaaagaagga taagtttaca ccttcttgac
    24241 aagattgtaa aaggaaggga agtctgatgg cagaagtaga agagttacga gtacaacctc
    24301 aagatatctt agctgagcaa tccgttttag gggctatctt tattgatgag agtaaacttg
    24361 tttttgtgcg agaatacatt gagtctcggg acttttttaa gtatgcccat cgtttgattt
    24421 tccaagccat ggtcgattta tccgatcgtg gtgatgccat agatgcaaca acggttcgta
    24481 ctatccttga taatcaaggt gatttacaga atattggtgg cttgtcttac ttggttgaga
    24541 ttgttaattc tgtgccaact tctgctaatg cggagtatta tgctaagatt gttgcagaaa
    24601 aagcaatgct acgtcgttta attgccaagt tgacagagtc tgtcaaccaa gcttacgaag
    24661 cgtcacaacc agctgatgaa attattgctc aggcagaaaa agggttgatt gatgtcagtg
    24721 aaaatgcaaa tcgaagcggg tttaagaaca ttcgagatgt gttgaatctc aactttggaa
    24781 atctggaagc tcgctcgcaa caaacgaccg atattacagg tattgcgaca ggttatcgtg
    24841 atttggatca tatgacaaca ggacttcatg aggaggagtt gattatctta gcagctcgtc
    24901 cagcagttgg taagacagca tttgccttga atatcgctca gaatattggg actaagttgg
    24961 acaaaacggt tgctattttt tcactcgaaa tgggtgcgga aagcttggta gatcgtatgt
    25021 tagctgcaga aggcttagtg gagtcacatt ctatccgtac agggcaattg acagatgagg
    25081 agtggcaaaa atatactatt gctcagggta atctagctaa cgccagtatc tatatcgatg
    25141 atacgccagg tattcggatt acagagattc gttctcgttc tcgtaaattg gctcaagaaa
    25201 ctggaaatct tggtttgatt ttgatagact atttgcaact tatcacggga actggtcgag
    25261 aaaatcgtca acaagaagtt tctgaaattt ctcgtcagtt gaaaatacta gccaaggaat
    25321 tgaaggttcc agtaatcgct ctgagtcagc tttctcgtgg tgtagaacaa cgtcaggaca
    25381 agagaccggt cttgtctgat attcgtgaat ctgggtctat tgagcaggac gctgatatcg
    25441 tagcttttct ctatcgcgat gactactatg aacgtggtgg tgaagaagag gagggtatcc
    25501 caaataataa ggtggaagtt attatcgaga aaaaccgtag tggagctcgt ggaacagtgg
    25561 aattgatttt ccaaaaagaa tacaataaat tttcaagtat ctcaaagagg gaggcataag
    25621 atgtcagatg catttacaga tgtagccaag atgaaaaaaa tcaaagaaga aatcaaggca
    25681 catgagggac aagtcgtaga aatgactttg gagaatggtc gtaagcgcca aaaaaataga
    25741 ttgggtaagc taattgaagt ttatccatct ctatttattg tggagtttgg gaatgtggaa
    25801 ggagataaac aagttaatgt ttacgttgaa tcctttactt actcagatat tcttacagaa
    25861 aagaatttga ttcattatct tgactaaagt gagaaatttt ctcacttttt cttttttctc
    25921 cgaataattt aggtgaaggc aatcatcgct ttatattatt tttcaaggag gaagaatgaa
    25981 aattttaccg tttatagcaa gagggacaag ttattacttg aagatgtcag ttaaaaagct
    26041 tgttcctttt ttagtagtag gattgatgct agcagctggt gatagtgtct atgcctattc
    26101 cggaggaaat ggatcgattg cgcgtgggga tgattatcct gcttattata aaaatgggag
    26161 ccaggagatt gatcagtggc gcatgtattc tcgtcagtgt acttcttttg tagcctttcg
    26221 tttgagtaat gtcaatggtt ttgaaattcc ggcagcttat ggaaatgcga atgaatgggg
    26281 acatcgtgct cgtcgggaag gttatcgtgt agataataca ccgacgattg gttccattac
    26341 ttggtctact gcaggaactt atggtcatgt tgcctgggtg tcaaatgtaa tgggagatca
    26401 gattgagatt gaggaatata actatggtta tacagaatcc tataataaac gagttataaa
    26461 agcaaacacg atgacaggat ttattcattt taaagatttg gatggcggca gtgttgggaa
    26521 tagtcaatcc tcagcttcaa caggcggaac tcattatttt aagaccaagt ctgctatcaa
    26581 aactgaaccc ctagctagcg gaactgtgat tgattactat tatcctgggg agaaggttca
    26641 ttatgatcag atacttgaaa aagacggcta taagtggttg agttatactg cctataatgg
    26701 aagctatcgt tatgttcaat tggaggctgt gaataaaaat cctctaggta attctgttct
    26761 ttcttcaaca ggtggaactc attattttaa gaccaagtct gctatcaaaa ctgaacccct
    26821 agttagcgga actgtgattg attactatta tcctggggag aaggttcatt atgatcagat
    26881 acttgaaaaa gacggctaca agtggttgag ttatacggct tataacggaa gtcgtcgcta
    26941 tatacagcta gagggagtga cttcttcaca aaattatcag aatcaatcag gaaatatctc
    27001 tagctatgga tccaataata gttcaactgt cggttggaag aaaataaatg gtagttggta
    27061 tcatttcaaa tcaaatggtt ctaaatcaac aggatggctg aaagacggtt ctagctggta
    27121 ttatttgaaa ttatctggtg aaatgcagac aggatggtta aaggaaaata gttcgtggta
    27181 ttatctgggt agttcagggg caatgaaaac aggctggtac caggtctctg gtaagtggta
    27241 ttattcttac tcttcaggcg ccttagctgt taatacgacg gtggatggct acagagtaaa
    27301 cagtgatgga gaacgagtat agaaaattgg agtaggagaa atttcctgct cttttctgta
    27361 agcagtttcc ttgacatttt tcttattttg cgctatcata tatacatata atatatggga
    27421 gtctgtgtac agtctgagag gaagtgttaa acttcgaccg cacctgatct gggtaatgcc
    27481 agcggaggga acgatactta gtctaatttt gcaccttttc catgtatggt aaaggttttt
    27541 ctttttttaa aaaggaaaac gagaagagga ggttcttatg aaagcaagca ttgccttgca
    27601 agttttaccc ctagcacagg ggattgatcg gatagctgtt attgatcagg tcattgctta
    27661 tctgcaaact caagaagtga cgatggtagt gacaccattt gaaacggtct tggaagggga
    27721 gtttgatgag cttatgcgca ttctaaaaga agcgctggaa gtggcagggc aggaggcaga
    27781 caatgtcttt gccaatgtca aaataaatgt aggagagatt ttaagtattg atgagaaact
    27841 tgaaaagtat aatgagacga cacattagtc tattgggctt tctcggagtc ttgttaatct
    27901 ggcagttagc aggttttctt aaacttctcc ccaagtttat cctgccgaca cctcttgaaa
    27961 ttctccagcc ctttgttcgt gacagagaat ttctctggca ccatagctgg gcgaccttga
    28021 gagtggcttt actggggctg attttgggag ttttgattgc ctgtcttatg gctgtgctca
    28081 tggatagttt gacttggctc aatgacctga tttaccctat gatggtggtc attcagacca
    28141 ttccgaccat tgccatagct cctatcctgg tcttgtggct aggttatggg attttgccca
    28201 agattgtctt gattatctta acgacaacct ttcccatcat cgttagtatt ttggacggtt
    28261 ttaggcattg cgacaaggat atgctgacct tgtttagtct gatgcgggcc aagccttggc
    28321 aaatcctgtg gcattttaaa atcccagtta gcctgcctta cttttatgca ggtctgaggg
    28381 tcagtgtctc ctacgccttt atcacaactg tggtatctga gtggttggga ggttttgaag
    28441 gtcttggtgt ttatatgatt cagtctaaaa aattgtttca gtatgatacc atgtttgcca
    28501 ttattattct ggtgtcgatt atcagtcttt tgggtatgaa gctggtcgat atcagtgaaa
    28561 aatatgtgat taaatggaaa cgttcgtaga attagaatgt ttctgaaaaa gaaaagagga
    28621 aatcaaaatg aagaaaacat ggaaagtgtt tttaacgctt gtaacagctc ttgtagctgt
    28681 tgtgcttgtg gcctgtggtc aaggaactgc ttctaaagac aacaaagagg cagaacttaa
    28741 gaaggttgac tttatcctag actggacacc aaataccaac cacacagggc tttatgttgc
    28801 caaggaaaaa ggttatttca aagaagctgg agtggatgtt gatttgaaat tgccaccaga
    28861 agaaagttct tctgacttgg ttatcaacgg aaaggcacca tttgcagtgt atttccaaga
    28921 ctacatggct aagaaattgg aaaaaggagc aggaatcact gccgttgcag ctattgttga
    28981 acacaataca tcaggaatca tctctcgtaa atctgataat gtaagcagtc caaaagactt
    29041 ggttggtaag aaatatggga catggaatga cccaactgaa cttgctatgt tgaaaacctt
    29101 ggtagaatct caaggtggag actttgagaa ggttgaaaaa gtaccaaata acgactcaaa
    29161 ctcaatcaca ccgattgcca atggcgtctt tgatactgct tggatttact acggttggga
    29221 tggtatcctt gctaaatctc aaggtgtaga tgctaacttc atgtacttga aagactatgt
    29281 caaggagttt gactactatt caccagttat catcgcaaac aacgactatc tgaaagataa
    29341 caaagaagaa gctcgcaaag tcatccaagc catcaaaaaa ggctaccaat atgccatgga
    29401 acatccagaa gaagctgcag atattctcat caagaatgca cctgaactca aggaaaaacg
    29461 tgactttgtc atcgaatctc aaaaatactt gtcaaaagaa tacgcaagcg acaaggaaaa
    29521 atggggtcaa tttgacgcag ctcgctggaa tgctttctac aaatgggata aagaaaatgg
    29581 tatccttaaa gaagacttga cagacaaagg cttcaccaac gaatttgtga aataatgaca
    29641 gaaattagac tagagcacgt cagttatgcc tatggtcagg agaggatttt agaggatatc
    29701 aacctacagg tgacttcagg cgaagtggtt tccatcctag gcccaagtgg tgttggaaag
    29761 accaccctct ttaatctaat cgctgggatt ttagaagttc agtcagggag aattgtcctt
    29821 gatggtgaag aaaatcccaa ggggcgcgtg agttatatgt tgcaaaagga tctgctcttg
    29881 gagcacaaga cggtgcttgg aaatatcatt ctgcccctct tgattcaaaa ggtggataag
    29941 gcagaagcta tttcccgagc ggataaaatt cttgcgacct tccagctgac agctgtaaga
    30001 gacaagtatc ctcatgaact tagcggtggg atgcgccagc gtgtagcctt actccggacc
    30061 tacctttttg ggcacaagct ctttctctta gatgaggcct ttagcgcctt ggatgagatg
    30121 acaaagatgg aactccacgc ttggtatctt gagattcaca aacagttgca gctaacaact
    30181 ctgatcatca cgcatagtat tgaggaggcc ctcaatctca gcgaccgcat ctatatcttg
    30241 aaaaatcgcc ctgggcagat tgtttcagaa attaaactag attggtctga agatgaggac
    30301 aaggaagtcc aaaagattgc ctacaaacgt caaatcttgg cagaattagg cttagataag
    30361 tagaaaaata gggagttggt gaagattatc ctttaccagc gccctttttc ttttaaaaat
    30421 gagaaaattt cggtataata gtcaaacaag gtcaaggttt aaagagagag gtgggtttgt
    30481 tatgagattt aaaaatacat cggatcatat tgaggcctac atcaaggcga ttttagatca
    30541 atctggtatc gtggagttgc aacggagtca gttggcagat acctttcagg ttgttcctag
    30601 tcagattaac tacgtgatca agacacgctt tacggaaagt agaggctact tggttgaaag
    30661 taagcgtggt ggcggaggct acattcgtat aggacggatt gagttttcta gtcatcatga
    30721 aatgctccgg gatctgcttt actcgattgg tgagcgagtc agtcaagaaa tttatgagga
    30781 tattctccag cttttggttg agcaggaatt gatgaccaag caggagatga atttgctaga
    30841 atcagtagct ttggatcgcg ttttaggaga agaagctcca gttgttcgag caaacatgct
    30901 acgtcaggtc atacaagagg tagatagaaa agggaagtaa gatgaactat tcaaaagcat
    30961 tgaatgaatg tatcgaaagt gcctacatgg ttgctggaca ttttggagct cgttatctag
    31021 agtcgtggca cttgttgatt gccatgtcta atcacagtta tagtgtagca ggggcaactt
    31081 taaatgatta tccgtatgag atggaccgtt tagaagaggt ggctttggaa ctgactgaaa
    31141 cggactatag ccaggatgaa acctttacgg aattgccgtt ctcccgtcgt ttgcaggttc
    31201 tttttgatga agcagagtat gtagcgtcag tggtccatgc taaggtacta gggacagagc
    31261 acgtcctcta tgcgattttg catgatggca atgccttggc gactcgtatc ttggagaggg
    31321 ctggtttttc ttatgaagac aagaaagatc aggtcaagat tgctgccctt cgtcgaaatt
    31381 tagaagaacg tgcaggctgg actcgtgaag atctcaaggc tttacgccaa cgccatcgta
    31441 cagtagctga caagcaaaat tctatggcca atatgatggg catgccgcag actcctagtg
    31501 gtggtctcga ggactatacg catgatttga cagagcaagc gcgttctagc aagttagaac
    31561 cagtcatcgg tcgggacaag gaaatctcac gtatgattca aatattgagc cgtaagacta
    31621 agaacaaccc tgtcttggtt ggggatgctg gtgtcgggaa aacagctctg gcccttggtc
    31681 ttgctcagcg tattgctagt ggtgacgtgc ctgcggaaat ggctaagatg cgcgtgttag
    31741 aacttgattt gatgaatgtc gttgcaggga cacgcttccg tggtgacttt gaagaacgca
    31801 tgaataatat catcaaggat attgaagaag atggccaagt catcctcttt atcgatgaac
    31861 tccacaccat catgggttct ggtagcggga ttgattcgac tctggatgcg gccaatatct
    31921 tgaaaccagc cttggcgcgt ggaactttga gaacggttgg tgccaccact caggaagaat
    31981 atcaaaaaca tatcgaaaaa gatgcggctc tttctcgtcg tttcgctaaa gtgacgattg
    32041 aagaaccaag tgtggcagat agtatgacta ttttacaagg tttgaaggcg acttatgaga
    32101 aacatcaccg tgtacaaatc acagatgaag cggttgaaac agcggttaag atggctcatc
    32161 gttatttaac cagtcgtcac ttgccagact ctgctatcga cctcttggac gaagcagcag
    32221 caacagtgca aaataaggca aagcatgtaa aagcagacga ttcagatttg agtccagctg
    32281 acaaggccct gatggatggc aagtggaaac aggcagccca gctaatcgca aaagaagagg
    32341 aagtacctgt ctacaaagac ttggtgacag agtctgatat tttgaccacc ttgagtcgct
    32401 tgtcaggaat cccagttcaa aaactgactc aaacggatgc taagaagtat ttaaatcttg
    32461 aagcagaact ccataaacgt gtcatcggtc aagatcaagc tgtttcaagt attagtcgtg
    32521 cgattcgccg taatcagtca ggaattcgca gtcacaagcg tccgatcggt tcctttatgt
    32581 tcctaggacc cacaggtgtc ggtaagaccg agttggccaa ggctttggca gaagttctct
    32641 ttgatgacga atcagccctt atccgctttg atatgagtga gtatatggag aaattcgcag
    32701 ccagccgtct aaatggagct cctccaggct atgtgggtta cgaagaaggt ggggagttga
    32761 cagagaaggt tcgcaataaa ccctattccg ttctcctctt tgatgaggta gagaaggctc
    32821 acccagatat ctttaatgtt ctcttgcagg ttctggatga cggtgtcttg acagatagca
    32881 agggacgcaa ggtcgatttt tcaaatacca ttatcattat gacatcgaat ctaggtgcga
    32941 ctgcccttcg tgatgataag actgttggtt ttggggctaa ggatattcgt tttgaccagg
    33001 aaaatatgga aaaacgcatg tttgaagaac tgaaaaaagc ttatagaccg gaattcatca
    33061 accgtattga tgagaaggtg gtcttccata gcctatctag tgaccatatg caggaagtgg
    33121 tgaagattat ggtcaagcct ttagtggcaa gtttggctga aaaaggcatt gacttgaaat
    33181 tacaagcttc agctctgaaa ttgttagcaa atcaaggata tgacccagag atgggagctc
    33241 gcccacttcg cagaaccctg caaacagaag tggaggacaa gttggcagaa cttcttctta
    33301 agggagattt agtggcaggc agcacactta agattggtgt caaagcaggc cagttaaaat
    33361 ttgatattgc ataaaagaat aaaagtatca gcatctgacc ataagtcaca gtggagtgaa
    33421 actcaatgaa aatcaaagag caaactaggc agctagccgc aggttgctta aaacactgtt
    33481 ttgaggttgc agatagagct gacgtggttt gaagagattt tcgaagagta tgaaactaaa
    33541 acctatagct tctaaacgat tcgtggtttt caccattcaa cacaaaattc atatgtttat
    33601 taccctccgt cgtatttgtc ttagagcgtg tgtagtagaa aaagagcagt cttatctgaa
    33661 atttttattc tttcaaaaga gacctgtttc ttttttgcat gtcaaatccg ttctaactgg
    33721 tatttgaaaa atcaaactaa tactcaatga aaatcaaaga acaaactagg cagctagccg
    33781 caggttgctc aaaacactgt tttgaggttg tagatagagc tgacgtggtt tggagagatt
    33841 ttcgaagagt ataagctgca agatgaatga ttttcttgta ttgacgttgt tgttgacaaa
    33901 aagtagcgga taaatgaaat ccattccatt atcatagatg ataggctggt aggaaatttt
    33961 caaatagcat acaggaaata gatgtatgga gttctggtag tagaaaggga gagagatgaa
    34021 cattttagtt gcagatgacg aggaaatgat tagagaagga attgcagcat ttctgacaga
    34081 agagggttat catgtcatta tggctaagga tggacaagag gtcttggaaa aatttcaaga
    34141 tctccctatc catctcatgg tactggattt aatgatgcct aggaagagtg gttttgaagt
    34201 gttaaaagaa atcaatcaaa agcacgatat tcctgtcatc gtcttgagtg ctctgggaga
    34261 tgaaactact cagtcacagg tatttgatct ctatgctgat gatcatgtga caaaaccttt
    34321 ttctttggta ctgcttgtca agcgtattaa ggcgcttatc agacgttact acgtcataga
    34381 ggatctttgg cgatatcagg atgtaacagt ggattttacc tcttacaaag cacattataa
    34441 aaatgaagaa attgatctca aaccaaagga attactggta ctaaagtgtt tgattcagca
    34501 taaaaatcaa gttttaagta gagagcagat attggaagaa atttcaaaag atgtagctga
    34561 tttaccttgt gatagggtcg ttgatgtcta tattcgtact cttcgcaaaa aattagcttt
    34621 agattgtatc gtgactgtga aaaatgttgg gtataagatt agcttatgat aaaaaatcct
    34681 aaattattaa ccaagtcttt tttaagaagt tttgcaattc taggtggtgt tggtctagtc
    34741 attcatatag ctatttattt gacctttcct ttttattata ttcaactgga gggggaaaag
    34801 tttaatgaga gcgcaagagt gtttacggag tatttaaaga ctaagacatc tgatgaaata
    34861 ccaagcttac tccagtctta ttcaaagtcc ttgaccatat ctgctcacct taaaagagat
    34921 attgtagata agcggctccc tcttgtgcat gacttggata ttaaagatgg aaagctatca
    34981 aattatatcg tgatgttaga tatgtctgtt agtacagcag atggtaaaca ggtaacagtg
    35041 caatttgttc acggggtgga tgtctacaaa gaagcaaaga atattttgct tttgtatctc
    35101 ccatatacat ttttggttac aattgctttt tcctttgttt tttcttattt ttatactaaa
    35161 cgcttgctca atcctctttt ttacatttca gaagtgacta gtaaaatgca agatttggat
    35221 gacaatattc gttttgatga aagtaggaaa gatgaagttg gtgaagttgg aaaacagatt
    35281 aatggtatgt atgagcactt gttgaaggtt attcatgagt tggaaagtcg taatgagcaa
    35341 attgtaaaat tgcaaaatca aaaggtttcc tttgtccgtg gagcatcaca tgagttgaaa
    35401 acccctttag ccagtcttag aattatccta gagaatatgc agcataatat tggagattac
    35461 aaagatcatc caaaatatat tgcaaagagt ataaataaga ttgaccagat gagccactta
    35521 ttggaagaag tactggagtc ttctaaattc caagagtgga cagagtgtcg tgagaccttg
    35581 actgttaagc cagttttagt agatatttta tcacgttatc aagaattagc tcattcaata
    35641 ggtgttacaa ttgaaaatca attgacagat gctaccaggg tcgtcatgag tcttagggca
    35701 ttggataagg ttttgacaaa cctgattagt aatgcaatta aatattcaga tgaaaatggg
    35761 cgcgtaatca tatccgagca agatggctat ctctctatca agaatacatg tgcgcctcta
    35821 agtgaccaag aactagaaca tttatttgat atattctatc attctcaaat cgtgacagat
    35881 aaggatgaaa gttccggttt gggtctttac attgtgagta atattttaga aagctatcaa
    35941 atggactata gttttctccc ttatgaacac ggtatggaat ttaagattag cttgtagaca
    36001 gattagtttt ttattaaagt tcacataggg ttaacataag tgtattattc tttatataga
    36061 taaaaatata gataaaagaa aggatactaa tatggtatta gcgattattt tagtaacatt
    36121 ccttattcga ttgatttttt taaagcgttc gatagagaat gagaaacgaa tccttagcaa
    36181 tggcgggaaa gaatttggag ttgagaatac aaaacgatta acgttggctc atattgtttt
    36241 ttatctctct tgcctcgttg aagcaatggt gcataagaca atgtttgatg gtatgagcat
    36301 ggttggttta gtcttgctta ttttttctat gctgatgttg atgttggtga ttcacttgtt
    36361 gggagatatt tggacagtga agcttatgct tgtcaataat cacaaatatg tagatcatat
    36421 cttgtttagg acagtaaaac atcctaatta ctttttaaat attttacctg agttgattgg
    36481 cttgaccttg ttgagtcatg cctatatgac ttttgtttta gtttttccag tttatgcagt
    36541 tattttgtat cgacgaatag ctgaagagga aaagctatta catgaagtta taatcccaaa
    36601 tggaagcata aagagataaa tacaaaattc gatttatata cagttcatat tgaagtgata
    36661 tagtaaggtt aaagaaaaaa tatagaagga aataaacatg tttgcatcaa aaagcgaaag
    36721 aaaagtacat tattcaattc gtaaatttag tattggagta gctagtgtag tagttgctag
    36781 tcttgttatg ggaagtgtgg ttcatgcgac ggagaatgag ggaattaccc aagtagccac
    36841 ttcttataat aaggcaaatg aaagtcagac agaacatagg aaagctgcta aacaagtcga
    36901 tgaagatata aaaaaaatgt tgagtgagat ccaagaatat ataaaaaaaa tgttgagtga
    36961 gatccaatta gataaaagaa aacataccca aaatgtcaac ttaaacagaa agttgagcgc
    37021 aattcaaacg aagtatttgt atgaattaag agttttaaaa gagaagtcga aaaaagaaga
    37081 gttgacgtca aaaacaaaaa aagagttaga cgcagctttt gagaagttta aaaaagaacc
    37141 agaacttact aaaaaattag cagaagctaa acaaaaagcc aaggctcaaa aagaagaaga
    37201 tttccgtaac tacccaacca atacttacaa aacgcttgaa cttgaaattg ctgagttcga
    37261 tgtgaaagtt aaagaagcgg agcttgaact agtaaaagag gaagctaaac cccgaaacga
    37321 ggaaaaaatt aagcaagcaa aagcgaaagt tgagagtaaa aaagctgagg ctacaaggtt
    37381 agaagaaatc aagacagaac gtaaaaaagc agaagaagaa gctaaacgaa aagcagaaga
    37441 atctgagaaa aaagctgctg aagccaaaca aaaagtggat actaaagagc aaggtaaacc
    37501 aaagaggcgg gcaaaacgag gagtttctgg agagctagca acacctgata aaaaagaaaa
    37561 tgatgcgaag tcttcagatt ctagcgtagg tgaagaaact cttccaagcc catcccttaa
    37621 tatggcaaat gaaagtcaga cagaacatag gaaagatgtc gatgaatata taaaaaaaat
    37681 gttgagtgag atccaattag atagaagaaa acatacccaa aatgtcaact taaacataaa
    37741 gttgagcgca attaaaacga agtatttgta tgaattaagt gttttaaaag agaactcgaa
    37801 aaaagaagag ttgacgtcaa aaaccaaagc agagttaacc gcagcttttg agcagtttaa
    37861 aaaagataca ttgaaaccag aaaaaaaggt agcagaagct gagaagaagg ttgaagaagc
    37921 taagaaaaaa gccaaggatc aaaaagaaga agatcgccgt aactacccaa ccaatactta
    37981 caaaacgctt gaacttgaaa ttgctgagtc cgatgtgaaa gttaaaaaag cggagcttga
    38041 actagtaaaa gaggaagcta acgaatctcg aaacgaggaa aaaattaagc aagcaaaaga
    38101 gaaagttgag agtaaaaaag ctgaggctac aaggttagaa aaaatcaaga cagatcgtaa
    38161 aaaagcagaa gaagaagcta aacgaaaagc agaagaatct gagaaaaaag ctgctgaagc
    38221 caaacaaaaa gtggatgctg aagaatatgc tcttgaagct aaaatcgctg agttggaata
    38281 tgaagttcag agactagaaa aagagctcaa agagattgat gagtctgact cagaagatta
    38341 tcttaaagaa ggcctccgtg ctcctcttca atctaaattg gataccaaaa aagctaaact
    38401 atcaaaactt gaagagttga gtgataagat tgatgagtta gacgctgaaa ttgcaaaact
    38461 tgaagttcaa cttaaagatg ctgaaggaaa caataatgta gaagcctact ttaaagaagg
    38521 tttagagaaa actactgctg agaaaaaagc tgaattagaa aaagctgaag ctgaccttaa
    38581 gaaagcagtt gatgagccag aaactccagc tccggctcct caaccagctc cagctccaga
    38641 aaaaccagct gaaaaaccag ctccagctcc agctccagaa aaaccagctc cagctccaga
    38701 aaaaccagca gaaaaaccag ctgaaaaacc agctccaact ccagaaactc caaaaacagg
    38761 ctggaaacaa gaaaacggta tgtggtactt ctacaatact gatggttcaa tggcaacagg
    38821 ctggctccaa aacaatggct catggtacta cctcaacagc aatggcgcta tggcgacagg
    38881 ctggctccaa aacaatggct catggtacta cctcaacagc aatggcgcta tggcgacagg
    38941 atggctccaa tacaatggt
//
LOCUS       DAVLVP010000008        38402 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.008, whole genome shotgun sequence.
ACCESSION   DAVLVP010000008 DAVLVP010000000
VERSION     DAVLVP010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 38402)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38402)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38402
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.008"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            3..75
                     /locus_tag="VYN69_000402"
     tRNA            3..75
                     /locus_tag="VYN69_000402"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Val,seq:tac)
     gene            78..150
                     /locus_tag="VYN69_000403"
     tRNA            78..150
                     /locus_tag="VYN69_000403"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Asp,seq:gtc)
     gene            175..247
                     /locus_tag="VYN69_000404"
     tRNA            175..247
                     /locus_tag="VYN69_000404"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:208..210,aa:Lys,seq:ttt)
     gene            253..334
                     /locus_tag="VYN69_000405"
     tRNA            253..334
                     /locus_tag="VYN69_000405"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:287..289,aa:Leu,seq:tag)
     gene            353..425
                     /locus_tag="VYN69_000406"
     tRNA            353..425
                     /locus_tag="VYN69_000406"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:386..388,aa:Thr,seq:tgt)
     gene            448..519
                     /locus_tag="VYN69_000407"
     tRNA            448..519
                     /locus_tag="VYN69_000407"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:480..482,aa:Gly,seq:gcc)
     gene            527..612
                     /locus_tag="VYN69_000408"
     tRNA            527..612
                     /locus_tag="VYN69_000408"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:561..563,aa:Leu,seq:taa)
     gene            622..695
                     /locus_tag="VYN69_000409"
     tRNA            622..695
                     /locus_tag="VYN69_000409"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:656..658,aa:Arg,seq:acg)
     gene            711..784
                     /locus_tag="VYN69_000410"
     tRNA            711..784
                     /locus_tag="VYN69_000410"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:745..747,aa:Pro,seq:tgg)
     gene            790..863
                     /locus_tag="VYN69_000411"
     tRNA            790..863
                     /locus_tag="VYN69_000411"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:824..826,aa:Met,seq:cat)
     gene            877..950
                     /locus_tag="VYN69_000412"
     tRNA            877..950
                     /locus_tag="VYN69_000412"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:911..913,aa:Ile,seq:cat)
     gene            961..1050
                     /locus_tag="VYN69_000413"
     tRNA            961..1050
                     /locus_tag="VYN69_000413"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:997..999,aa:Ser,seq:tga)
     gene            1064..1137
                     /locus_tag="VYN69_000414"
     tRNA            1064..1137
                     /locus_tag="VYN69_000414"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1098..1100,aa:Met,seq:cat)
     gene            1141..1213
                     /locus_tag="VYN69_000415"
     tRNA            1141..1213
                     /locus_tag="VYN69_000415"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1174..1176,aa:Phe,seq:gaa)
     gene            1233..1303
                     /locus_tag="VYN69_000416"
     tRNA            1233..1303
                     /locus_tag="VYN69_000416"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1265..1267,aa:Gly,seq:tcc)
     gene            1339..1412
                     /locus_tag="VYN69_000417"
     tRNA            1339..1412
                     /locus_tag="VYN69_000417"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1373..1375,aa:Ile,seq:gat)
     gene            1419..1506
                     /locus_tag="VYN69_000418"
     tRNA            1419..1506
                     /locus_tag="VYN69_000418"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1453..1455,aa:Ser,seq:gct)
     gene            complement(1632..2567)
                     /locus_tag="VYN69_000419"
     CDS             complement(1632..2567)
                     /locus_tag="VYN69_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     synthetase/biotin operon repressor"
                     /protein_id="HEV1532750.1"
                     /translation="MKSYQAVYQILSKETDYISGEKIAEKLSLSRTSIWKAIKRLEQE
                     GIEIDSIKNRGYKLMNGDLILPEILEENLPIKVSFKPETKSTQLDAKEAIDLGHEANT
                     LYLASYQTAGRGRFQRSFYSPQGGIYMTLHLKPNLPYDKLPSYTLLVAGAVYKAIKNL
                     TLIDVDIKWVNDIYLNNHKIGGILTEAMTSVETGLVTDIIIGVGINFTIKDFPQELKE
                     KAASLFKATAPITRNELIIEIWRAFFETPAEELLYLYKKQSFILGKEVTFTLEQKDYK
                     GLAKDISENGKLLVQCDNGKEIWLNSGEISLNSWK"
     gene            complement(2564..3424)
                     /locus_tag="VYN69_000420"
     CDS             complement(2564..3424)
                     /locus_tag="VYN69_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000959966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEV1532751.1"
                     /translation="MLVFSEYQTGTIDLALSFYGYEECTPNYSFGPAIRDTYVLHYIT
                     KGQGKFHYKGKIVDLKEGDFFLLKPEELTFYQADSKEPWAYYWLGITGGKSPDYFALS
                     QISDQSYLIQSETCHTQTTAKLISDIVRFAQITKSSELAQLHIMGQLHELMFHLGTIA
                     PNQKKKNISSTHQLYLECKRLIDSHYPQSLTIQDLAKELSVHRSYLSSVFKEFNTLSP
                     KEYLLYVRMHRARQLLENTQESIKVIAYSVGFSDPLHFSKAYKQYFNQTPSHTRKEYS
                     QYQLVRKATL"
     gene            3532..5694
                     /locus_tag="VYN69_000421"
     CDS             3532..5694
                     /locus_tag="VYN69_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000538915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEV1532752.1"
                     /translation="MGVRIENNLFYVESKNLSLIIENRNGYLLLKHLGKTIKNYKGSN
                     SVYERDHAFSGNPTATNRTFSLDTQRQIFGQHGLGDFRKPTIQVQHNVTEVTDFRFVE
                     AKILKGQNGPQGLPSPHSMDDTETLVLMLEDSKAQLSLTLYYTTFNNDATITSYSKLD
                     NNSNQEVVIHKDFSFMADFPAADYEIATLQGAYAREKTVRRQQVEQGIFSISSNRGAS
                     GHAQTPALLLCEQGVTEDAGNVFAIQLMYSGNFEAFVQKNQLNEVRVAIGINPENFSW
                     KLAPEEYFETPVALVTHSDQGLTGISHESQNFVLKHIMLSEFSKKERPILINNWEATY
                     FDFQREKLLELADEAKKAGIELFVLDDGWFGNRFDDNRALGDWVVNEEKLGGSLESLI
                     SAIHERGLQFGLWLEPEMISVDSDLYRQHPDWAIQVPGYEHTYSRNQLVLNLANPQVV
                     EYLKSVLDQLLSYHDIDYIKWDMNRNITKLGNGLTYLETQMQSHQYMLGLYELVSYLT
                     EKHSHILFESCSGGGGRNDLGMIRYFPQVWASDNTDAIARLPIQYGSSYLYPTISMGA
                     HVSAVPNHQMGRMTPLETRGHVAMMGNLGYELDLTNLSDEEKATIANQVNLYKELRPV
                     VQLGQQYRLINPDTASNEAAVQFNYGNQTIVTYVRVLSVVETMETTLKLKDLDEEGLY
                     KLQENGEVYSGAELMYAGLTVILSQGDFLSRQYIFRKL"
     gene            5786..7045
                     /locus_tag="VYN69_000422"
     CDS             5786..7045
                     /locus_tag="VYN69_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1532753.1"
                     /translation="MEWYKKIGLLATTGLALVGLGACSNYGKSADGTVTIEYFNQKKE
                     MTKTLEGITRDFEKENSKIKVKVVNVPNAGEVLKTRVLAGDVPDVVNIYPQSIELQEW
                     AKAGVFEDLSNKDYLKRVKNGYAEKYAVNEKVYNVPFTANAYGIYYNKDKFEELGLKV
                     PETWDEFEQLVKDIVAKGQTPFGIAGADAWTLNGYNQLAFATATGGGKEANQYLRYSQ
                     PNAIKLSDPIMKDDIKVMDILRINGSKQKNWEGAGYTDVIGAFARGDVLMTPNGSWAI
                     TAINEQKPNFKIGTFMIPGKEKGQSLTVGAGDLAWSISATTKHPKEANAFVEYMTRPE
                     VMQKYYDVDGSPTAIEGVKQAGEDSPLAGMTEYAFTDRHLVWLQQYWTSEADFHTLTM
                     NYVLTGDKQGMVNDLNAFFNPMKADVD"
     gene            7059..7925
                     /locus_tag="VYN69_000423"
     CDS             7059..7925
                     /locus_tag="VYN69_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1532754.1"
                     /translation="MKKVLQKYWAWAFVVIPLLLQAIFFYVPMFQGAFYSFTNWTGLT
                     YNYKFVGLNNFKLLFMDPKFMNAIGFTAIITIAMVVGEIALGIFIARVLNSKIKGQTF
                     FRAWFFFPAVLSGLTVALIFKQVFNYGLPAIGNALHIEFLQTSLLGTKWGAIFAAAFV
                     LLWQGVAMPIIIFLAGLQSIPTEITEAARIDGATSKQVFWNIELPYLLPSVSIVFILA
                     LKGGLTAFDQVFAMTGGGPNNATTSLGLLVYNYAFKNNQFGYANAIAVILFFLIVVIS
                     IIQLRVSKKFEI"
     gene            7939..8772
                     /locus_tag="VYN69_000424"
     CDS             7939..8772
                     /locus_tag="VYN69_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1532755.1"
                     /translation="MKQDERKALIGKYILLILGSVLILVPLLATLFSSFKPTKDIVDN
                     FFGFPTNFTWDNFSRLLADGIGGYYWNSVVITVLSLLAVMIFIPMAAYSIARNMSKRK
                     AFTIMYTLLILGIFVPFQVIMIPITVMMSKLGLANTFGLILLYLTYAIPQTLFLYVGY
                     IKISIPESLDEAAEIDGANQFTTYFRIIFPMMKPMHATTMIINALWFWNDFMLPLLVL
                     NRDSKMWTLPLFQYNYAGQYFNDYGPSFASYVVGIISITIVYLFFQRHIIAGISNGAV
                     K"
     gene            9092..10534
                     /gene="gtfA"
                     /locus_tag="VYN69_000425"
     CDS             9092..10534
                     /gene="gtfA"
                     /locus_tag="VYN69_000425"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEV1532756.1"
                     /translation="MPIQNKIMLITYSDSLGNNLKDLYDNLEEHFGDAIGGVHLLPFF
                     PSTGDRGFAPVDYDEVDSAFGDWEDVKRLGERYYLMFDFMINHISRQSKYYKDYQEKH
                     EASEFKALFLNWDKFWPENRPTQSDVDLIYKRKDRAPKQEIVFEDGSVEHLWNTFGEE
                     QIDLDVTKEVTMEFIRKTIQHLASNGCDLIRLDAFAYAVKKLDTNDFFVEPDIWDLLD
                     KVRDIAAEYGTELLPEIHEHYSIQFKIADHDYYVYDFALPMVTLYTLYSSRTERLAKW
                     LKMSPMKQFTTLDTHDGIGVVDVKDILTDEEIDYASNELYKVGANVKRKYSSAEYNNL
                     DIYQINSTYYSALGDDDVKYFLARLIQAFAPGIPQVYYVGLLAGKNDLKLLEETKEGR
                     NINRHYYSNEEIAKEVQRSVVKALLNLFSFRNRSEAFDLEGTTEIETPTAQSIVIKRQ
                     NKDKSVTAVAEIDLQNQTYRVIENGVEVTF"
     gene            10672..11865
                     /locus_tag="VYN69_000426"
     CDS             10672..11865
                     /locus_tag="VYN69_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008293041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEV1532757.1"
                     /translation="MKSIGFIEKLKGLSSKELILLGIILSIFLPFYLFVVVLCLYIIS
                     LIFTGDMKSILQKMGEHPMLLLFLSYSTVISILAQNWMGLVASVGMFLFTIFFLHYQS
                     ILSHKFFRLILQFVLFGSVLSAAFASLEHFQIVKKFNYAFLSPNMQVWHQNRAEVTFF
                     NPNYYGIICCFCIMIAFYLFTTTKLNWLKVFCVIAGFVNLFGLNFTQNRTAFPAIIAG
                     AIIYLFTTIKNWKAFWLSIGVFAIGLSFLFSSDLGVRMGTLDSSMEERISIWDAGMAL
                     FKQNPFWGEGPLTYMHSYPRIHAPYHEHAHSLYIDTILSYGIVGTILLVLSSVAPVRL
                     MMDMSQESGKRPIIGLYLSFLTVVAVHGIFDLALFWIQSGFIFLLVMCSIPLEHRMLV
                     SDMTD"
     gene            12551..14530
                     /locus_tag="VYN69_000427"
     CDS             12551..14530
                     /locus_tag="VYN69_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000742236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1532758.1"
                     /translation="MKKNRVFATAGLVLLAAGVLAACSSSKSSDSSAPKAYGYVYTAD
                     PETLDYLISSKNSTTVVTSNGIDGLFTNDNYGNLAPAVAEDWEVSKDGLTYTYKIRKG
                     VKWFTSDGEEYAEVTAKDFVNGLKHAADKKSEAMYLAENSVKGLADYLSGTSTDFSTV
                     GVKAVDDYTLQYTLNQPEPFWNSKLTYSIFWPLNEEFETSKGSDFAKPTDPTSLLYNG
                     PFLLKGLTAKSSVEFVKNEQYWDKENVHLDTINLAYYDGSDQESLERNFTSGAYSYAR
                     LYPTSSNYSKVAEEYKDNIYYTQSGSGIAGLGVNIDRQSYNYTSKTTDSEKVATKKAL
                     LNKDFRQALNFALDRSAYSAQINGKDGAALAVRNLFVKPDFVSAGEKTFGDLVAAQLP
                     AYGDEWKGVNLADGQDGLFNADKAKAEFAKAKKALEADGVQFPIHLDVPVDQASKNYI
                     SRIQSFKQSVETVLGVENVVVDIQQMTSDEFLNITYYAANASSEDWDVSGGVSWGPDY
                     QDPSTYLDILKTTSSETTKTYLGFDNPNSPSVVQVGLKEYDKLVDEAAKETSDLNVRY
                     EKYAAAQAWLTDSSLFIPAMASSGAAPVLSRIVPFTGASAQTGSKGSDVYFKYLKLQD
                     KVVTKEEYEKAREKWLKEKAESNEKAQKELASHVK"
     gene            14597..16093
                     /locus_tag="VYN69_000428"
     CDS             14597..16093
                     /locus_tag="VYN69_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1532759.1"
                     /translation="MKKYIFMRVLRSLVSIFLVTTLTYTIIYTLVPRKLIFKQDPNYN
                     KIATTADKRDNYENTVFERMGYIEYYDTKELQEKASSMDSSVTVEANATNKAIYEKYI
                     NQLGHGWTLGEFTESGQFYATREIPIFERVFHFYANLIDIDHTNKIQDPENPDLKRYL
                     RFENDPAIGWSLVGSGTKHKYLLYFNSQFPFVHQNFVNLNLGDSYPTYANTPVLQVIT
                     QGQGQTKTAQVQFPTGKKTSSVNIYSRTYKSPSQADSREVASYGKDDPYTATESNYQY
                     PSMIVSSAITGLIGLVLAYALAVPLGSAMARFKNTWIDSLSTGALTFLLALPTIALVY
                     IVRLIGSSISLPDSFPILGAGDWRSYVLPAVILGLLGAPGTAIWIRRYMIDLQSQDFV
                     RFARAKGLSEKEISNKHIFKNAMVPLVSGIPGAIIGVIGGATLTETVFAFPGMGKMLI
                     DSVKASNNSMVVGLVFIFTCISIFSRLLGDIWMTIIDPRIKLTEKGGK"
     gene            16093..17019
                     /locus_tag="VYN69_000429"
     CDS             16093..17019
                     /locus_tag="VYN69_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1532760.1"
                     /translation="MSTIDKEKFQFVKRDDFASETIDAPAYSYWKSVFKQFMKKKSTV
                     VMLGILVAIILISFIYPMFSKFDFNDVSKVNDFSVRYIKPNAEHWFGTDSNGKSLFDG
                     VWFGARNSILISVIATVINLVIGVFVGGIWGISKSVDRVMMEVYNVISNIPPLLIVIV
                     LTYSIGAGFWNLIFAMSVTTWIGIAFMIRVQILRYRDLEYNLASRTLGTPTLKIVAKN
                     IMPQLVSVIVTTMTQMLPSFISYEAFLSFFGLGLPITVPSLGRLISDYSQNVTTNAYL
                     FWIPLTTLVLVSLSLFVVGQNLADASDPRTHR"
     gene            17028..18095
                     /locus_tag="VYN69_000430"
     CDS             17028..18095
                     /locus_tag="VYN69_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1532761.1"
                     /translation="MTKEKNVILTARDIVVEFDVRDKVLTAIRGVSLELVEGEVLALV
                     GESGSGKSVLTKTFTGMLEENGRIAQGSIDYRGQDLTALSSHKDWEQIRGAKIATIFQ
                     DPMTSLDPIKTIGSQITEVIVKHQGKTAKEAKELAIDYMNKVGIPDADRRFNEYPFQY
                     SGGMRQRIVIAIALACRPDVLICDEPTTALDVTIQAQIIDLLKSLQNEYHFTTIFITH
                     DLGVVASIADKVAVMYAGEIVEYGTVEEVFYDPCHPYTWSLLSSLPQLADDKGDLYSI
                     PGTPPSLYTDLKGDAFALRSDYAMQIDFEQKAPQFSVSETHWAKTWLLHEDAPKVEKP
                     AVIANLHDKIREKMGFAHLAD"
     gene            18106..19032
                     /locus_tag="VYN69_000431"
     CDS             18106..19032
                     /locus_tag="VYN69_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1532762.1"
                     /translation="MSEKLVEIKDLEISFGEGSKKFVAVKNANFFINKGETFSLVGES
                     GSGKTTIGRAIIGLNDTSNGDIIFDGQKINGKKSREQAAELIRRIQMIFQDPAASLNE
                     RATVDYIISEGLYNHRLFKDEEERKEKVQSIIREVGLLAEHLTRYPHEFSGGQRQRIG
                     IARALVMQPDFVIADEPISALDVSVRAQVLNLLKKFQKELGLTYLFIAHDLSVVRFIS
                     DRIAVIYKGVIVEVAETEELFNNPIHPYTQALLSAVPIPDPILERKKVLKVYDPSQHD
                     YETDKPSMVEIRPGHYVWANQAELARYQKGLN"
     gene            19651..20460
                     /locus_tag="VYN69_000432"
                     /pseudo
     CDS             19651..20460
                     /locus_tag="VYN69_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813348.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            20472..21365
                     /locus_tag="VYN69_000433"
     CDS             20472..21365
                     /locus_tag="VYN69_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1532763.1"
                     /translation="MNFVLSSLSEGLLWSIVAIGDYLTFRILDIADMTAEGAFPLGAA
                     VVVSQIQAGANPWLATLLALLAGMVAGLVSGMLHTKMKIPALLTGIVTLTGLYSINIK
                     IMGSVPNLSLGDSATVFKQLASLGLTNEGAVFSLSLVCFLLVCLVLTLLMKTEIGLVL
                     RSTGDNIPMSEANGVNVDTMKIVGYMISNGLIALCGSLFAQNDGFSDVTSGTGTIVVG
                     LSSVIIAEVLIHDLTIGGRLLSIGIGAIVYRLIILNIYEIPNLDQNLVRLFNAILLTL
                     VLFAPELQKRLKIRGLKLRNE"
     gene            21378..22181
                     /locus_tag="VYN69_000434"
     CDS             21378..22181
                     /locus_tag="VYN69_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000157668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1532764.1"
                     /translation="MASLLTLENIHKTFEAGTVNENHVLKGLDLEVEEGDFISVIGGN
                     GAGKSTLMNILAGNLSVDEGDLLLAGKSIKNLSVRKRAKDIARVFQDPKMGTASSLTI
                     EENMAIALRRGQKRGLGWGVKEKDRIQFQEALKELNIGLENRLKVDTQYLSGEQRQAL
                     TLVMAALVKPKLLLLDEHTAALDPKTSQMVMDLTQKIVEHHQWTTLMITHDMNHAIEY
                     GNRLIMLYQGKIVVDVKGEEKKHLTVEDLMHLFQKNSGQSLVSDELVLG"
     gene            complement(22214..22924)
                     /gene="treR"
                     /locus_tag="VYN69_000435"
     CDS             complement(22214..22924)
                     /gene="treR"
                     /locus_tag="VYN69_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEV1532765.1"
                     /translation="MKKYQQLFKQIQETIQNETYAVGDFLPSEHDLMEQYQVSRDTVR
                     KALSLLQEEGLIKKIRGQGSQVVKEETVNFPVSNLTSYQELVKELGLRSKTNVVSLDK
                     IIIDKKSSLITGFPEFRMVWKVVRQRVVDDLVSVLDTDYLDMELIPNLTRQIAEQSIY
                     SYIENGLKLLIDYAQKEITIDHSSDRDKILMDIGKDPYVVSIKSKVYLQDGRQFQFTE
                     SRHKLEKFRFVDFAKRKK"
     gene            23109..25076
                     /gene="treP"
                     /locus_tag="VYN69_000436"
     CDS             23109..25076
                     /gene="treP"
                     /locus_tag="VYN69_000436"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEV1532766.1"
                     /translation="MGKFEQEAKDLLQAIGGKENVTAVTHCATRMRFVLGDDKKANVK
                     AIESIPAVKGTFTNAGQFQVIIGNDVPIFYNDFTAVSGIEGVSKEAAKSAAKSNQNVV
                     QRVMTTLAEIFTPIIPALIVGGLILGFRNVLEGVHWSMLDGKTITESSQFWAGVNHFL
                     WLPGEAIFQFLPVGITWSVSRKMGTSQILGIVLGICLVSPQLLNAYAVASTPAADIEA
                     NWVWNFGYFTVNRIGYQAQVIPALLAGLSLSYLEIFWRKHIPEVISMIFVPFLSLIPA
                     LILAHTVLGPIGWTIGQGLSSVVLAGLTGPVKWLFGAIFGALYAPFVITGLHHMTNAI
                     DTQLIADAGGTALWPMIALSNIAQGSAVFAYYFMHRHDEREAQVSLPATISAYLGVTE
                     PALFGVNVKYIYPFVAGMTGSALAGMLSVTFNVTAASIGIGGLPGILSIQPQYMLPFA
                     GTMLVAIVVPMLLTFFFRKAGLFTKTEGDTNLQAEFVAQEEAEFVSHELVELTSVEII
                     SPLTGQVKELSQATDPVFASGVMGQGLVIEPSQGELTSPVNGTVTVLFPTKHAIGIVS
                     DEGVELLIHIGMDTVGLDGKGFESLVVQGDHVTVGQQLIRFDMDVIKAAGLVTETPVI
                     ITNQDAYTATITGTYPTTIQAGASLMVATRI"
     gene            25254..26879
                     /gene="treC"
                     /locus_tag="VYN69_000437"
     CDS             25254..26879
                     /gene="treC"
                     /locus_tag="VYN69_000437"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEV1532767.1"
                     /translation="MTLDKGKVVYQIYPKSYKDTTENGIGDFRGIIEKIPYLAKLGVD
                     MVWLNPFYPSPQRDNGYDISDYMAVDPLFGDMADFEEMVCVGKEHKIDFMLDMVLNHC
                     STEHEWFQKALAGDKYYQDFFFIQDQPTDWQSKFGGSAWAPFGDTGKYYLHLFDETQA
                     DLNWRNPNVRKELFKVVNFWRDKGVKGFRFDVINLIGKDEVSVDCPENEGKPAYTDKP
                     IVHNYLRMMNQATFGSDDSFMTVGEMSSTTMENCVLYSSPDRQELSMTFNFHHLKVDY
                     KDGQKWTLAPFDFEELKSLYHSWGKEMSDKDGWSALFWNNHDQPRALNRFVDIQNFRK
                     EGATMLAASIHLSRGTPYIYMGEEIGMIDPDYDSMADYVDVESLNAYQMLLEEGKSQQ
                     EAFQIIQAKSRDNSRIPMQWDASENAGFSTGTPWLKAGKSYKYINVENEIQGPIFTFY
                     QDLIRLRKEMPIISEGSYKPAFEDSKQVYAFERQFEDQKLLVLNNFYAKEVEIDLPAV
                     YQNGQILISNYEDAEVSEKILLKPYQTLAIYVN"
     gene            27135..27383
                     /locus_tag="VYN69_000438"
     CDS             27135..27383
                     /locus_tag="VYN69_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEV1532768.1"
                     /translation="MDLLLAIVLIVLAFLGGALGGMYLVRKQIEKEFADNPRLNAEAV
                     RTLLSANGQKPSEAKVQQVYHQIIRQQKAALANNKKKK"
     gene            27590..28384
                     /gene="racE"
                     /locus_tag="VYN69_000439"
     CDS             27590..28384
                     /gene="racE"
                     /locus_tag="VYN69_000439"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEV1532769.1"
                     /translation="MDNRPIGFLDSGVGGLTVVRELMRQLPHEEIVYIGDSARAPYGP
                     RPAEQIREYTWQLVNFLLTKDVKMIVIACNTATAVVWEEIKAQLDIPVLGVILPGASA
                     AIKSSQGGKIGVIGTPMTVQSDIYRQKIHDLDPDLQVESLACPKFAPLVESGALSTSV
                     TKKVVYETLRPLVGKVDSLILGCTHYPLLRPIIQNVMGPKVQLIDSGAECVRDISVLL
                     NYFEINRGRDAGPLHHRFYTTASSQSFAQIGEEWLEKEIHVEHVEL"
     gene            28381..29352
                     /locus_tag="VYN69_000440"
     CDS             28381..29352
                     /locus_tag="VYN69_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000182414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphate diphosphatase"
                     /protein_id="HEV1532770.1"
                     /translation="MTNKIYEYKDDQDWYVGSYSIFGGVNSLSDYKTDFPLFEFSKIF
                     GDEEYGFPLSVTVLRYGSTYRLFSFVVDMLNQEMGRNLEVIQRHGALLLVENGQLLYV
                     ELPKEGVNVHDFFETSKVRETLLIATRNEGKTKEFRAIFDKLGYDVENLNDYPDLPEV
                     AETGMTFEENARLKAETISQLTGKMVLADDSGLKVDVLGGLPGVWSARFAGVGATDRE
                     NNAKLLHELAMVFELKDRSAQFHTTLVVASPNKESLVVEADWSGYINFEPKGENGFGY
                     DPLFLVGETGESSAELTLEEKNSQSHRALAVKKLLEVFPSWQSKPSL"
     gene            29328..29849
                     /locus_tag="VYN69_000441"
     CDS             29328..29849
                     /locus_tag="VYN69_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEV1532771.1"
                     /translation="MAKQTIIVMSDSHGDSLIVEEVRDRYVGKVDAVFHNGDSELRPD
                     SPLWEGIRVVKGNMDFYAGYPERLVTELGSTKIIQTHGHLFDINFNFQKLDYWAQEEE
                     AAICLYGHLHVPSAWLEGKILFLNPGSISQPRGTIRECLYARVEIDDSYFKVDFLTRD
                     HEVYPGLSKEFSR"
     gene            29846..30307
                     /locus_tag="VYN69_000442"
     CDS             29846..30307
                     /locus_tag="VYN69_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1532772.1"
                     /translation="MIAKEFETFLLGQEETFLTPAKNLAVLIDTHNADHATLLLSQMT
                     YTRVPVVTDEKQFVGTIGLRDIMAYQMEHDLSQEIMADTDIVHMTKTDVAVVSPDFTI
                     TEVLHKLVDESFLPVVDAEGIFQGIITRKSILKAVNALLHDFSKEYEIRCQ"
     gene            30298..31038
                     /gene="xerD"
                     /locus_tag="VYN69_000443"
     CDS             30298..31038
                     /gene="xerD"
                     /locus_tag="VYN69_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEV1532773.1"
                     /translation="MPMRDRISVFLEEKQGLSVNSKQSYKYDLEQFLDMVGERISETS
                     LKIYQAQLANLKISAQKRKISACNQFLYFLYQKGEVDSFYRLELAKQAEKKTEKPEIL
                     YLDSFWQESDHPEGRLLALLILEMGLLPSEILAIKVADINLDFQVLRISKASQQRIVT
                     IPTALLSELEPLMGQTYLFERGEKPYSRQWAFRQLESFVKEKGFPSLSAQVLREQFIL
                     RQIENKVDLYEIAKKLGLKTVLTLEKYR"
     gene            31038..31766
                     /locus_tag="VYN69_000444"
     CDS             31038..31766
                     /locus_tag="VYN69_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEV1532774.1"
                     /translation="MDIKLKDFEGPLDLLLHLVSKYQMDIYDVPITEVIEQYLAYVST
                     LQAMRLEVTGEYMVMASQLMLIKSRKLLPKVAEVTDLGDDLEQDLLSQIEEYRKFKLL
                     GEHLEAKHQERAQYYSKAPTELIYEDAELVHDKTTIDLFLAFSNILAKKKEEFAQNHT
                     TILRDEYKIEDMMIIVKESLVGRAQLRLQDLFKEAQNVQEVITLFLATLELIKTQELI
                     LVQEESFGDIYLMEKKEESQVPQS"
     gene            31785..32354
                     /locus_tag="VYN69_000445"
     CDS             31785..32354
                     /locus_tag="VYN69_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein B"
                     /protein_id="HEV1532775.1"
                     /translation="MSTLAKIEALLFVAGEDGIRVRQLAELLSLPPTGIQQSLGKLAQ
                     KYEKDPDSSLALIETSGAYRLVTKPQFAEILKEYSKAPINQSLSRAALETLSIIAYKQ
                     PITRIEIDAIRGVNSSGALAKLQAFDLIKEDGKKEVLGRPNLYVTTDYFLDYMGINHL
                     EELPVIDELEIQAQESQLFGERIEEDENQ"
     gene            32341..33063
                     /locus_tag="VYN69_000446"
     CDS             32341..33063
                     /locus_tag="VYN69_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1532776.1"
                     /translation="MRINKYIAHAGVASRRKAEELIKQGLVTVNGQVVRELATTIKSG
                     DKVEVEGQPIYNEEKVYYLLNKPRGVISSVTDDKGRKTVVDLLPNVKERIYPVGRLDW
                     DTSGVLILTNDGDFTDEMIHPRNEIDKVYVARVKGVANKDNLRPLTRGLEIDGKKTKP
                     AVYEILKVDPVKNRSVVQLTIHEGRNHQVKKMFEAVGLQVDKLSRTRFGHLDLTGLRP
                     GESRRLNKKEISQLHTMAVTKK"
     gene            33063..33305
                     /gene="yidD"
                     /locus_tag="VYN69_000447"
     CDS             33063..33305
                     /gene="yidD"
                     /locus_tag="VYN69_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEV1532777.1"
                     /translation="MKRILIAPVRFYQRFISPVFPPSCRFELTCSNYMIQAIEKHGFK
                     GVLMGLARILRCHPWSKTGKDPVPDHFSLKRNQEGE"
     gene            complement(33530..34495)
                     /locus_tag="VYN69_000448"
     CDS             complement(33530..34495)
                     /locus_tag="VYN69_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1532778.1"
                     /translation="MKTSLKLYFTALVASFLLLLGACSTNSSTSQTETSSSAPTEITI
                     KSSLDEVKLSKVPEKIVTFDLGAADTIRALGFEKNIVGMPTKTVPTYLKDLVGTVKNV
                     GSMKEPDLEAIAALEPDLIIASPRTQKFVDKFKEIAPTVLFQASKDDYWTSTKANIES
                     LASAFGETGTQKAKEELAKLDKSIQEVATKNESSDKKALAILLNEGKMAAFGAKSRFS
                     FLYQTLKFKPTDTKFEDSRHGQEVSFESVKEINPDILFVINRTLAIGGDNSSNDGVLE
                     NALIAETPAAKNGKIIQLTPDLWYLSGGGLESTKLMIEDIQKALK"
     gene            complement(34591..35343)
                     /locus_tag="VYN69_000449"
     CDS             complement(34591..35343)
                     /locus_tag="VYN69_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000764290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1532779.1"
                     /translation="MKLENIDKSIQKQDILQGISLEVSPQKLTAFIGPNGAGKSTLLS
                     IMSRLTKKDQGVLSIKGREIESWNSQELAQELTILKQKINYQAKLTVEELVSFGRFPY
                     SRGRLRSEDWEKIRETLNYLELTNLKDRYINSLSGGQLQRVFIAMVLAQDTDFILLDE
                     PLNNLDIKQSVSMMQILRRLVEELGKTIIIVLHDINMASQYADEIVAFKDGQVFSKGR
                     TDQIMQADLLSQLYEIPITLADINDKKICIYS"
     gene            complement(35340..36296)
                     /locus_tag="VYN69_000450"
     CDS             complement(35340..36296)
                     /locus_tag="VYN69_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEV1532780.1"
                     /translation="MQTKSKHTKLFWILIILAIGACLLYFWPITHLSAFAWKLRSQKI
                     IVYLLVAIATGISTISFQTLTENRFLTPSILGIESFYVLLQTLLLVFESKFLQLGKSP
                     ILEFLVLLLVQSFFFLALQGYLKTLMKQDLVFILLICLALRSLFRNISTFLQVLMDPN
                     EYDKLQNSLFASFQHLNTSILAIGSLIILALTIFFFRKAVVLDVLHLQRETAQILGLD
                     VEKEQKELLWGIVLLTSTATALVGPMAFFGFMLANLTYLIVKDYQHKLLFIVAILVGF
                     ISLTLGQALIERVFALEIRISMIIESVGGFLFFILLYRRARQ"
     gene            complement(36286..37245)
                     /locus_tag="VYN69_000451"
     CDS             complement(36286..37245)
                     /locus_tag="VYN69_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1532781.1"
                     /translation="MKLSHYLIGLLLLLVFLSISIGTSDFSWGKLFDFDQQTWLLFQE
                     SRLPRTISILLTASSMSMAGLLMQTITQNQFAAPSTVGTTEAAKLGMVLSLFVFPSAS
                     LTQKMLFAFVSSIVFTLFFLAFMTIFTVKERWMLPLIGIIYSGIIGSVTEVIAYRFNL
                     VQSMTAWTQGSFSMIQTHQYEWLFLGLIILITVWKLSQTFTIMNLGKETSESLGISYS
                     LLEKLALFLVALTTSVTMITVGGLPFLGVIVPNLVRKRYGDNLSQTKLMVALVGANLV
                     LACDILSRVLIRPYELSVSLLLGIIGSLVFILLLWRGGRKDAD"
     gene            complement(37668..38369)
                     /gene="galE"
                     /locus_tag="VYN69_000452"
     CDS             complement(37668..38369)
                     /gene="galE"
                     /locus_tag="VYN69_000452"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1532782.1"
                     /translation="MTKTILVTGGTSYIGSHTVKALLNAGYQVHVLDNLSTGNRAAVD
                     SRASFKELDVYDASALKAYLEENQIDAVLHCAGEIVVSESIENPSKYFTANVAGMNQV
                     LKVLSEVGIQKIMFSSTASLYGNNCIDKPMTEDTLLDPVNPYAETKLMGERMIYWMAN
                     RYDWKYVIFRYFNVAGAEMDASNGLRVKNPTHIIPNINKTALGQHDSLKIFGDDYDTR
                     DGSCIRDYIYVLDLA"
BASE COUNT        10999 a         7220 c         8618 g        11565 t
ORIGIN      
        1 tagggagttt agctcagctg ggagagcatc tgccttacaa gcagagggtc agcggttcga
       61 tcccgttaac tcccaaaggt cccgtagtgt agcggttatc acgtcgccct gtcacggcga
      121 agatcgcggg ttcgattccc gtcgggaccg tttaaggtaa cgcaagttat tttagactcg
      181 ttagctcagt tggtagagca attgactttt aatcaatggg tcactggttc gagcccagta
      241 cgggtcatat atgcgggttt ggcggaattg gcagacgcac cagatttagg atctggcgct
      301 taacggcgtg ggggttcaag tcccttaacc cgcattaaga tataacaaat gagccggctt
      361 agctcagttg gtagagcatc tgatttgtaa tcagagggtc gcgtgttcaa gtcatgtagc
      421 cggcattttt agatagaaga aaacagtgcg aacgtagttc agtggtagaa caccaccttg
      481 ccaaggtggg ggtcgcgggt tcgaatcccg tcgttcgctt agagaggccg gggtggcgga
      541 actggcagac gcacaggact taaaatcctg cgattggtaa cgatcgtacc ggttcgattc
      601 cggtcctcgg cataatatgg agcaccctta gctcaactgg atagagtacc tgactacgaa
      661 tcaggcggtt agaggttcga ctcctctagg gtgcattttt ctatttaact cgggaagtag
      721 ctcagcttgg tagagtactt ggtttgggac caaggtgtcg caggttcgaa tcctgtcttc
      781 ccgattcatg gcggtgtagc tcagctggct agagcgtccg gttcataccc gggaggtcgg
      841 gggttcgatc cccttcgccg ctataatgat cttgtcggac ctttagctca gctggttaga
      901 gctctcggct cataaccgag tggtcgtagg ttcaagtcct acaaggtcca tttgaatatt
      961 ggaggattac ccaagtccgg ctgaagggaa cggtcttgaa aaccgtcagg cgtgtaaaag
     1021 cgtgcgtggg ttcgaatccc acatcctcct tttatattat taacgcggga tggagcagct
     1081 cggtagctcg tcgggctcat aacccgaagg tcgtaggttc aaatcctgct cccgcaataa
     1141 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cggttcgatt
     1201 ccgtctcgcg ccattttatt gatagtattt atgcgggtgt agtttagtgg taaaactaca
     1261 gccttccaag ctgttgtcgc gagttcgatt ctcgtcaccc gctttgaact ttgttcaaat
     1321 taccaagttt tttaacttgg gcgcgtagct caggtggtta gagcgcacgc ctgataagcg
     1381 tgaggtcggt ggttcgagtc cactcgtgcc cattaattgg agaattactc aagaggctga
     1441 agaggacggt ttgctaaatc gttaggtcgg gtaactggcg caagggttcg aatcccttat
     1501 tctccgtttt aatgagagtt tgtaactctc ttttttattt ttagagacaa taaaacttca
     1561 gatccgtaaa acttaattga attactaatc tgaagttatt tttatatcgt ttatattata
     1621 attgtgttat tttacttcca actattgaga gaaatttcgc cactatttag ccagatttct
     1681 tttccgttat cacattgaac taaaagtttt ccattttctg agatgtcttt agcaagtccc
     1741 ttgtagtctt tttgctctag tgtgaaagtg acttcttttc ctagaatgaa tgactgtttt
     1801 ttgtataggt ataatagctc ttctgctggt gtttcgaaga aagcacgcca gatttctatg
     1861 atcaattcat tccttgttat aggagctgta gctttaaata agctggcagc tttttctttt
     1921 aattcctgag ggaagtcttt aatagtgaaa ttgataccta ctccaataat gatatctgtg
     1981 actaagccag tttctacaga ggtcattgct tcagtaagga ttcctccaat tttatgattg
     2041 tttagataga tatcattgac ccattttatg tcgacatcta ttaaagttag gttcttaata
     2101 gctttgtaga cagctccagc tacaagtagt gtgtaggatg gtaatttgtc ataggggaga
     2161 tttggtttaa gatggagtgt catataaata ccaccttgtg gtgagtagaa ggaacgttga
     2221 aaacggcctc ggcctgctgt ttgataggaa gctagataga gggtatttgc ttcatggcct
     2281 aaatcaattg cttcttttgc atctagttgt gttgattttg tttcgggttt aaagctgact
     2341 ttaattggaa gattttcttc tagaatctct ggaagaataa ggtcaccatt catcagttta
     2401 tatcctctat ttttgatact atcaatttca atgccttctt gttctagtcg cttgatagct
     2461 ttccaaattg atgttcggct tagggatagt ttttctgcga ttttttctcc gctgatatag
     2521 tcggtttctt tagataggat ttggtagaca gcttggtagg atttcataat gttgcctttc
     2581 tcactagttg gtattgagag tattcttttc ttgtatgact tggagtctga ttaaagtatt
     2641 gtttataagc tttcgaaaaa tggagtggat ctgaaaaacc taccgagtat gcaattacct
     2701 tgatggactc ttgggtattt tcgagaagtt gtctagctcg gtgcattcga acgtagagta
     2761 ggtattcttt gggtgataag gtattaaatt ctttgaatac gcttgataag tagcttctgt
     2821 gaacggatag ttcttttgct aaatcttgaa ttgtaagtga ttgaggatag tggctatcaa
     2881 ttaatcgttt gcattcaaga tagagttggt gggttgatga aatattcttt tttttctgat
     2941 tgggagcaat agttcccaga tgaaacatca gttcatgaag ttgtcccatg atatggagtt
     3001 gagctaattc acttgatttt gtaatctgag cgaagcggac aatgtctgag atgagttttg
     3061 cagtagtctg ggtatgacaa gtttcagatt ggatgagata ggattgatca gaaatttgag
     3121 aaagagcaaa ataatcaggg gatttccctc cagtgattcc taaccagtag taggcccaag
     3181 gttctttact atctgcttga taaaaggtta gttcctctgg ttttaataga aagaaatctc
     3241 cttcttttaa atcaacaatt ttacccttgt aatgaaattt tccttgtcct ttagtaatgt
     3301 aatgtaggac gtatgtatca cgaatggctg gaccaaaaga gtaattaggt gtgcattcct
     3361 catatccata aaagcttagg gcaaggtcaa ttgttccagt ctggtattct gaaaaaacta
     3421 acatgtgcta cctcctacct aacattttac catattcttg agacattttt ctattttaga
     3481 aagcgatttc aggtgataaa atataatcaa taaagtgatg aggtgaagta aatgggagtt
     3541 aggatagaga ataatctatt ttatgttgag agtaaaaatc taagtttgat tattgaaaat
     3601 cgaaatggct acttactttt gaaacattta ggaaagacta ttaagaacta taaaggttcc
     3661 aatagtgttt atgaacgaga ccatgccttt tcaggaaatc caacagctac taatcgaacc
     3721 tttagtttag atactcaacg tcaaattttt ggacaacatg gcttaggaga ttttaggaaa
     3781 ccaaccatac aggttcagca taatgtaact gaagtaacag actttcgatt tgtagaagca
     3841 aagattttaa aaggtcagaa tggtccacag ggcttacctt ctccacatag catggacgat
     3901 acagagactc ttgtcttaat gttagaagat tctaaggctc aacttagtct gactttgtat
     3961 tatactactt ttaataatga tgcgactatt actagctaca gtaaattaga taataatagt
     4021 aatcaggaag ttgtcatcca taaagatttt tcttttatgg ctgattttcc agctgcagat
     4081 tatgaaatag caactctgca gggtgcttat gctcgtgaaa agactgttag acgtcaacag
     4141 gtagaacaag gaatcttttc gattagttca aaccgaggtg cttctggtca tgctcaaaca
     4201 ccagctcttc tactatgcga acaaggagtc acagaggatg ctgggaatgt gtttgctatt
     4261 caactaatgt atagtggcaa ctttgaagct tttgttcaaa aaaatcaatt gaatgaagtt
     4321 cgggtggcta ttggcattaa tccagaaaac ttttcttgga agttagcccc tgaggaatac
     4381 tttgaaacac cggtagcttt agtgactcat tcagatcagg gattaactgg tattagtcat
     4441 gaaagtcaga attttgtact gaagcacatt atgctaagtg aattttctaa aaaagaacgt
     4501 ccaattctaa tcaataactg ggaagctact tactttgact ttcagagaga aaaactgtta
     4561 gagttagcag atgaagctaa gaaagctggc attgaacttt ttgtattaga tgatggttgg
     4621 tttggcaatc gttttgatga taatcgtgct ttaggtgatt gggttgttaa tgaggaaaaa
     4681 ctgggtggaa gtctagaaag tctgatttca gctatccatg aaagaggttt gcagtttgga
     4741 ctttggttag aacccgaaat gatttctgta gatagtgatt tgtatcgtca acatcctgac
     4801 tgggctattc aggttcctgg ctatgagcat acttattctc ggaatcaatt agtacttaat
     4861 cttgccaatc ctcaggtagt agaatacttg aaaagtgtct tagatcaact cctatcttat
     4921 catgatattg attacattaa atgggatatg aaccgcaata tcactaagct agggaatgga
     4981 ttaacttatc tagagacaca gatgcaatct catcagtaca tgctggggct ttacgaactc
     5041 gtttcttatc tgacagagaa gcacagccat attctctttg agtcctgctc tggtggtggt
     5101 ggacgaaatg atcttggtat gatacgctat ttcccacaag tctgggctag tgataatact
     5161 gatgccattg cacgtttacc aattcaatac ggttcatcct atctctatcc aaccatttct
     5221 atgggggctc atgtgtcagc agtaccgaat catcagatgg gacgaatgac accattagaa
     5281 acacgtggcc atgtagcaat gatgggaaat ttgggctatg agcttgattt gacaaattta
     5341 tcagatgaag agaaagctac gattgctaat caggtgaact tgtataaaga attacgacca
     5401 gtagttcagt taggacagca gtatagacta attaatcctg atactgcatc caatgaagca
     5461 gctgtacaat ttaattacgg aaatcaaacg attgtaacct acgttcgcgt tttatctgtt
     5521 gtagagacca tggaaacaac tttaaaatta aaagatttgg atgaagaggg actatataaa
     5581 ttacaggaaa atggcgaagt ttactcaggt gcagaactaa tgtatgcggg cttaactgtt
     5641 atcttatccc aaggagattt tttgagtaga cagtatattt ttagaaaact atgatatgta
     5701 ctgaccccaa aagttagatt tattctgtct aacttttggg gtgcagttca ataagggtgt
     5761 ataaatttat aaaggaggct ttctaatgga atggtataaa aaaatcggac ttcttgcaac
     5821 tacaggttta gctttggttg ggctcggcgc ttgctccaac tatggtaaat ctgcggatgg
     5881 cacagtaacc atcgagtatt tcaaccagaa aaaagaaatg accaaaacct tggaaggaat
     5941 cactcgtgat tttgagaagg aaaactctaa gatcaaggtc aaagtcgtca atgtaccaaa
     6001 tgctggtgaa gtattgaaga cacgcgttct cgcaggagat gtgcctgatg tggtcaatat
     6061 ttacccacag tccatcgaac tgcaagaatg ggcaaaagca ggtgtctttg aagatttgag
     6121 caacaaagac tacctgaaac gcgtgaaaaa tggctacgct gaaaaatatg ctgtaaacga
     6181 aaaagtttac aacgttcctt ttacagctaa tgcttatgga atttactaca acaaagataa
     6241 attcgaagaa ctgggcttga aggttcctga aacctgggat gaatttgaac agttagtcaa
     6301 agatatcgtt gctaaaggac aaacaccatt tggaattgca ggtgcagatg cttggacact
     6361 caatggttac aatcaattag cctttgcgac agcaacaggt ggaggaaaag aagcaaatca
     6421 ataccttcgt tattctcaac caaatgccat taaattgtcg gatccgatta tgaaagatga
     6481 tatcaaggtc atggacatcc ttcgcattaa tggctctaag caaaagaact gggaaggtgc
     6541 tggctatacc gatgttatcg gagccttcgc acgtggggat gtcctcatga caccaaatgg
     6601 gtcttgggcg atcacagcga ttaatgaaca aaaaccgaac tttaagattg ggaccttcat
     6661 gattccagga aaagaaaaag gacaaagctt aaccgttggt gcgggagact tggcatggtc
     6721 tatctcagcc accaccaaac atccaaaaga agccaatgcc tttgtggaat atatgacccg
     6781 tccagaagtc atgcaaaaat actacgatgt ggacggatct ccaacagcga tcgaaggggt
     6841 caaacaagca ggagaagatt caccgcttgc tggtatgacc gaatatgcct ttacggatcg
     6901 tcacttggtc tggttgcaac aatactggac cagtgaagca gacttccata ccttgaccat
     6961 gaactatgtc ttgaccggtg ataaacaagg catggtcaat gatttgaatg ccttctttaa
     7021 cccgatgaaa gcggatgtgg attaggagta gagagactat gaaaaaagta ttacaaaaat
     7081 attgggcatg ggcttttgtg gtcatccccc tcttgttaca agcaattttc ttctatgtgc
     7141 cgatgtttca aggagccttt tacagtttta ccaactggac aggattgact tataactaca
     7201 aatttgtcgg cttaaacaac tttaagctcc tcttcatgga tccaaaattc atgaatgcga
     7261 ttggctttac cgcaatcatt acgattgcca tggtggttgg tgagattgca ctcgggatct
     7321 tcattgcgcg tgtcttgaac tctaaaatca aaggccaaac cttcttccgt gcttggttct
     7381 tcttcccagc tgttttatct ggtttgacag tggctttgat cttcaagcaa gtcttcaact
     7441 acggtcttcc agcgattggg aatgcccttc atattgaatt tctccaaacc agtcttttag
     7501 ggactaagtg gggagcaatc tttgcggctg cctttgtcct tctttggcaa ggggtggcta
     7561 tgcccatcat catcttccta gctggtttgc aatctattcc aactgagatt acagaggcag
     7621 caaggattga tggtgcgact agcaagcaag ttttctggaa cattgaattg ccttacttgc
     7681 taccaagtgt ctctatagtc tttatcctag ccctaaaagg tgggctgact gcctttgacc
     7741 aagtctttgc catgaccggt ggtggtccaa acaatgccac aacctcactt gggctcttgg
     7801 tttataacta tgcctttaaa aacaaccaat tcggttatgc caatgccatt gccgtaatct
     7861 tgttcttctt aattgtagtg atttcgatca tccaattgag agtatctaag aaatttgaaa
     7921 tttaagagga gaagcatgat gaaacaagat gaaagaaaag ccctgattgg caaatacatt
     7981 ctattgattc taggatcggt tctgatttta gtgccgctcc ttgctaccct ctttagttcc
     8041 ttcaaaccca ctaaggatat tgtagataat ttctttggct ttccaaccaa cttcacatgg
     8101 gacaacttta gccgtctctt agctgatggg attggaggct attattggaa ctctgtcgtc
     8161 atcactgtct tgtctttact tgcagtaatg atctttatcc ctatggcagc ctactccatc
     8221 gctcgcaata tgagtaaaag aaaagccttt accatcatgt ataccctctt aatcctcgga
     8281 atattcgtac ctttccaagt catcatgatt ccgattacgg ttatgatgag taaactcggt
     8341 ttggctaata cctttggttt gatcttgctc tacttgacct atgcgattcc acagaccctc
     8401 tttctctatg ttggctatat caaaatctcg attccagaaa gtctggatga agcagcagag
     8461 atcgatgggg ctaatcaatt tacaacctat ttccgcatca tcttcccaat gatgaaaccg
     8521 atgcatgcga caaccatgat catcaatgcc ctttggttct ggaatgactt catgttgcca
     8581 ctccttgtct tgaaccggga ttccaaaatg tggactctgc ctttgttcca atacaactac
     8641 gcaggccaat atttcaacga ttacggacca agctttgcct cttacgtggt cggcattatc
     8701 agtatcacca ttgtctatct cttcttccaa cgccatatca tcgcaggaat aagcaacggg
     8761 gcagtgaagt aagaagagct catagaatct atagtctctg acacagagga agagttcagc
     8821 tagttgacaa ttttatgtga aaacaagaaa gtagtactta gtttattgaa gtctatattt
     8881 agtacgagtt tgatgataaa tagagattta atactcaatg aaaatcaaag agcaaactag
     8941 gaagctagcc gcaggctgct caaaacactg ttttgaggtt gcagatagaa ctgacgtggt
     9001 ttgaagagat tttcgaagag tataaaatga atttgattta cgaagccaga tcatatgaga
     9061 cttggcttca attagaaaaa ggagagtaat gatgccaatt caaaataaaa tcatgttgat
     9121 tacctattct gatagccttg gaaataatct taaagactta tatgataatt tggaagagca
     9181 ttttggagat gctattggag gagttcacct tttaccattt ttcccatcaa caggtgatcg
     9241 tggatttgcg ccagttgact acgacgaagt ggactcagct tttggtgatt gggaggatgt
     9301 taagcgttta ggtgagagat attatcttat gtttgatttt atgattaatc atatttctcg
     9361 tcaatccaag tattataagg actatcaaga aaaacatgaa gccagtgaat ttaaagctct
     9421 ctttttaaac tgggataagt tttggccaga aaaccgtccg acacagtctg atgtagattt
     9481 aatttacaag cgtaaggatc gtgcaccaaa gcaagagatt gtgtttgaag atggttcagt
     9541 ggaacatttg tggaatacct ttggtgagga gcagattgat cttgatgtga ccaaagaagt
     9601 aactatggaa tttatccgta agaccattca gcacttggca agtaatgggt gtgatttgat
     9661 tcgtctagat gcctttgctt atgcagtgaa gaaattggat actaatgatt tctttgtgga
     9721 accagatatt tgggatttat tggacaaagt tcgagatatc gctgctgagt atgggacaga
     9781 gcttttacct gagattcatg aacactattc gattcagttt aaaatagcag accatgatta
     9841 ctatgtttat gattttgctc ttccaatggt gacactttat actctttaca gttccagaac
     9901 agagcgtttg gctaagtggt taaagatgag cccgatgaag caatttacga cgctagatac
     9961 ccatgatggg attggagtag tagatgtcaa ggatatcctg accgatgagg agattgacta
    10021 tgcttcaaat gaactctata aggttggagc caatgtcaaa cgtaagtact ctagtgccga
    10081 gtataacaac ttagatatct accaaatcaa ttcaacctac tattcagcgc ttggagatga
    10141 tgatgtcaag tattttctcg ctcgtctaat tcaagctttt gccccaggta ttcctcaggt
    10201 ttactatgtg ggtctattag caggcaagaa tgacttgaaa ttattagaag aaactaaaga
    10261 aggtcgaaat attaatcgtc attactatag caacgaggaa atagcaaaag aagtgcaacg
    10321 atctgttgtg aaggcccttc tcaatctatt ttctttccgt aaccgttcag aagcctttga
    10381 tctagaaggg actactgaga tagagacacc aacagctcag agcattgtaa tcaaacgtca
    10441 aaataaagat aagtccgtaa cagcagtagc agaaattgat ttgcaaaatc agacttatcg
    10501 ggtaattgag aatggagttg aagtaacatt ttgaagcctt gatatggtgg ataaaatagg
    10561 gtttttattt tggaaaacgt ttcctttgtt ttcaaattgc taaaaaagtg gtacaatata
    10621 ggatagctta ctattatctg aatcagctga tttggagaga aaggattcat tttgaaatca
    10681 ataggcttta ttgaaaagct gaaggggttg tctagtaaag agctgatttt attgggaatt
    10741 atcctgagta tctttttacc cttttatctt tttgtagttg tactctgttt atatattatc
    10801 agtttgattt ttacaggaga catgaaaagt attcttcaga aaatggggga gcatccgatg
    10861 ctgcttcttt ttcttagcta tagtactgtt atatccattc ttgcacaaaa ttggatgggt
    10921 cttgtggctt cagtaggaat gtttctattt actattttct ttttgcacta tcagtcgatt
    10981 ttatcccata aattctttcg attgattttg cagttcgtct tgtttggtag tgtcttgtca
    11041 gctgcttttg ccagtttaga acatttccaa attgtgaaga aatttaacta tgcttttctt
    11101 tcacccaata tgcaggtgtg gcatcagaac cgggcagaag tgaccttctt taatcctaat
    11161 tattatggaa ttatttgttg tttctgtatt atgattgctt tctatctgtt tacaacgacc
    11221 aagttgaatt ggttgaaagt attctgtgtg attgcaggct ttgttaatct ctttggtttg
    11281 aactttactc aaaatcgaac tgcctttcct gctattatcg ctggagcaat tatctatctc
    11341 tttacgacta ttaaaaactg gaaggccttt tggcttagta ttggggtctt cgcgattggt
    11401 ttgagtttcc tcttttctag tgatttggga gttcgaatgg gtactttaga ctcttctatg
    11461 gaagaacgca tttctatctg ggatgctggg atggccttgt ttaagcaaaa tcctttttgg
    11521 ggtgaagggc cattgaccta tatgcactct tatcctcgga tacatgctcc ttatcatgaa
    11581 catgcccaca gtctttatat tgatacgatt ctgagttacg gaattgtggg taccatttta
    11641 ttagttttgt cttctgttgc tcctgttcgc ttgatgatgg atatgagtca ggagtcgggg
    11701 aaacgtccga ttatcggcct ttatctatct ttccttacag tggttgctgt gcacggaatt
    11761 tttgacttgg ctctcttctg gattcagtca ggctttattt tcttgctagt tatgtgcagt
    11821 attccattgg agcatcgaat gttggtatcg gacatgacgg attaagtttt ataagtttga
    11881 aatcttctac cttgggtagg agattttttt atacgatggg catgtcgtgc atctgaggtg
    11941 taagtcctct gtagggatcc actaccggtg aatacaatag cgctccccaa gcctgactat
    12001 cgtgaggtag cggattaaaa tggtctgggg atagatcgtt ttaagtctga cgcaggaaat
    12061 aagaattgtc agagaaagca atagtgaaat cgtaggctat gaatagaaac gtgatagcga
    12121 ggagcatata gtggataagg tagcgagaaa ttttaaagta taaaagatag tcttaatcta
    12181 tatatgtaaa tcacaaaagt aattgaattc ggaataaggt tggtgtgaaa agacatagta
    12241 aactgaatct tgaatactag aatgatactt ctacaatatt gttataattt aatttgaatt
    12301 ccaaaatcac attgttccgt ccgtatttca ttttactgta tgtcttccta aactccaaag
    12361 tttctgcgtc aaatttccta ttttcactgt agtctttgac acttcgaacg acaatttgaa
    12421 tcatatattc aaaaaaaatg aaaaatttgt gccaacaagc ctaaatattc tgaaaatttg
    12481 tttgattttt aatggataat gtgatataat ggttgtcaat ctataattca cataaacaga
    12541 ggagtagaaa atgaaaaaaa atcgtgtatt tgctacagca ggtcttgttt tattagcagc
    12601 aggtgtactt gcagcatgca gttcttcaaa atcatctgat tcatcagccc ctaaagctta
    12661 tggctatgtt tatacagcag acccagaaac cttggactac ctgatttcaa gtaaaaatag
    12721 tacaacagta gtgacttcaa atgggattga tggtttattc actaacgata attacggtaa
    12781 tcttgctcct gcagttgcag aggattggga agtctctaag gatggtttga cctacactta
    12841 taagattcgt aaaggggtta aatggtttac ctctgatgga gaagaatatg cagaggtgac
    12901 ggctaaagat ttcgtgaacg gtttaaaaca cgcagcagat aaaaaatcag aagctatgta
    12961 tttagctgaa aattcggtta aaggcttggc agattatcta tcaggaactt caacagattt
    13021 ttcaacagtt ggtgtcaagg cggttgatga ttatacgtta caatacactt tgaaccagcc
    13081 tgaaccgttc tggaactcta agttgaccta ttctattttc tggcctctga atgaagaatt
    13141 cgaaacatca aaaggaagcg attttgctaa accaacagat ccgacatcct tgctttataa
    13201 tggtccattc ttgttgaaag ggttgactgc aaaatcttct gtagagtttg taaaaaatga
    13261 gcaatattgg gataaagaaa atgtccacct agatactatc aatctagctt actatgatgg
    13321 atcagatcag gagtcgctag agcgtaactt cactagtgga gcttatagtt atgcccgtct
    13381 ttaccctacc agctccaact attctaaggt tgcagaagaa tacaaggaca atatctatta
    13441 cacacaatca ggctctggga ttgctggtct gggtgtgaat attgatcgcc aaagttacaa
    13501 ctatacttct aaaactacag attcagagaa agtagctact aagaaggcat tgcttaacaa
    13561 agatttccgt caagccttga attttgctct tgatcgctca gcttactcag ctcaaatcaa
    13621 tggtaaagat ggagcagctt tagcagttcg taatttattt gtaaaaccag actttgtttc
    13681 agctggtgag aagacctttg gtgatttagt cgctgctcaa cttcctgctt atggtgatga
    13741 gtggaaaggt gtgaatttag ctgatgggca ggatggttta ttcaatgctg acaaggccaa
    13801 ggcagagttt gcgaaagcta agaaagcttt agaagcagac ggcgttcagt ttcctattca
    13861 tctggacgtt ccagtagacc aagcatcaaa aaactacata tctcgtattc agtcctttaa
    13921 acaatctgta gaaacagttc ttggtgttga aaatgtcgtt gttgatattc aacaaatgac
    13981 aagtgatgaa ttccttaata ttacttacta tgctgccaat gcttcatctg aggattggga
    14041 tgtatcagga ggagtttcat gggggccaga ctatcaagac ccatctactt acctggatat
    14101 tttaaaaaca actagcagtg aaactacaaa aacatattta ggatttgata atccaaatag
    14161 cccttcagta gttcaagttg gtttgaaaga atacgataaa ttagttgatg aagctgccaa
    14221 agagacaagc gacttgaatg tccgttatga aaaatatgca gcggctcaag catggttgac
    14281 agatagttca ctctttattc ctgctatggc ttcttctggt gcagcaccag tgctttcacg
    14341 aattgttcca tttactggag cttctgcgca aacaggctct aaggggtcag atgtttactt
    14401 caaatatttg aaattacaag ataaagtggt gactaaggaa gagtatgaaa aagctcgtga
    14461 aaaatggttg aaagaaaaag ctgaatcaaa tgagaaagct caaaaagaat tggcaagtca
    14521 tgtgaagtaa ataatttcta atagaacttt ctctccatga ggagaaggtt cttttgggat
    14581 ttttaaagga aataatatga aaaaatatat ttttatgcgt gttttgcggt cattggtttc
    14641 gattttctta gtaacgactt tgacctacac gattatctat accttggttc ctcgaaaatt
    14701 gattttcaag caggatccta actataataa aattgcgaca acggctgata aacgtgataa
    14761 ctatgaaaat actgtgtttg agcgtatggg ctacattgag tattacgata ctaaagagtt
    14821 gcaagaaaag gcaagtagca tggattcttc tgtaacagta gaagcaaatg cgaccaataa
    14881 agctatttat gaaaagtaca tcaatcaatt aggtcatggt tggactttgg gagaatttac
    14941 tgaaagtggt caattctatg ctactcgtga aattccaatt tttgaacgtg tttttcactt
    15001 ctatgctaac ttgattgaca ttgaccatac aaataaaatc caagaccctg aaaatccaga
    15061 cttgaaacgc taccttcgtt ttgaaaatga tccagctatc ggatggtcat tggtcggttc
    15121 aggaactaaa cataaatatc tcttgtactt taacagtcag ttcccatttg ttcatcaaaa
    15181 ctttgtgaac ttgaatttag gtgactctta cccaacctat gctaatacac cagttcttca
    15241 ggttattact caaggtcaag gacaaaccaa aactgcccaa gttcagttcc caacaggtaa
    15301 gaaaacgtct tctgtaaata tttactcaag aacctacaag tcacctagtc aggctgactc
    15361 tcgtgaagta gctagctatg ggaaagatga tccttataca gcgactgaaa gtaattacca
    15421 atatccatct atgattgtca gctctgctat tactggtttg attggtttgg ttcttgccta
    15481 tgctcttgcc gtgccacttg gttcagccat ggctcgtttc aagaacactt ggattgatag
    15541 cctctcaaca ggggctttga ccttcttgct tgctcttcca acgattgcct tggtttacat
    15601 cgttcgattg attggatcat ctatttccct tccagattca ttccctatct tgggagctgg
    15661 agattggcgt tcttacgttt taccagcagt catccttggt ttgttgggtg ctcctggtac
    15721 agccatttgg attcgtcgtt acatgattga cttgcaatct caagactttg ttcgtttcgc
    15781 tcgtgcaaaa ggtttgtctg aaaaagaaat ttcaaacaaa cacatcttta aaaatgccat
    15841 ggttccgctg gtttcaggaa ttcctggtgc cattattggg gttatcggtg gtgcaaccct
    15901 tactgaaaca gtcttcgcct tcccaggtat gggtaaaatg ttgattgact ctgtaaaagc
    15961 atctaataac tctatggtcg ttggtcttgt cttcatcttt acatgtattt ctatcttctc
    16021 acgtcttttg ggagatattt ggatgactat tattgaccca cgtattaaat tgactgagaa
    16081 aggaggcaaa taatgtctac aatcgataaa gaaaaatttc agtttgtaaa acgtgacgat
    16141 tttgcctctg aaactattga tgcgccagca tattcttact ggaaatcagt gtttaaacaa
    16201 tttatgaaga aaaaatcaac tgtagtcatg ttgggaatct tggtagccat cattttgata
    16261 agcttcatct acccaatgtt ttctaagttt gatttcaatg atgtcagcaa ggtaaacgac
    16321 tttagtgttc gttatatcaa gccaaatgcg gagcattggt tcggtactga cagtaacggt
    16381 aaatcgctct ttgacggtgt ctggttcgga gctcgtaact ccatcctcat ttctgtgatt
    16441 gcgacagtga ttaacttggt tatcggtgtt tttgtcggtg gtatttgggg tatttcaaaa
    16501 tcagttgacc gtgtcatgat ggaagtttac aacgtcatct caaacatccc acctcttttg
    16561 attgttattg tcttgactta ctcaatcgga gctggattct ggaatctgat ttttgccatg
    16621 agcgtaacaa catggattgg tattgccttc atgatccgtg tgcaaatctt gcgctatcgt
    16681 gacttggaat acaacttggc gtcacgtact ttgggaacac caaccttgaa gattgttgcc
    16741 aaaaatatca tgcctcaatt ggtatctgtt attgtgacaa ccatgactca aatgcttcca
    16801 agctttatct catacgaagc cttcttgtct ttcttcggtc ttggattacc gattacagtg
    16861 ccaagtttgg gtcgtttgat ttcggattat tcacaaaacg taacaaccaa tgcttacttg
    16921 ttctggattc cattgacaac ccttgtcttg gtatccttgt cccttttcgt agttggtcaa
    16981 aacttagcgg atgctagtga tccacgtaca catagatagg agtagaaatg acaaaagaaa
    17041 aaaatgtaat tttgactgct cgcgatattg tcgtggaatt tgacgttcgt gacaaagtat
    17101 tgacagccat tcgcggcgtt tcccttgaac tagtcgaagg agaagtatta gccttggtag
    17161 gtgagtcagg atcaggtaaa tctgttttga caaagacctt cacaggtatg ctcgaagaaa
    17221 atggtcgtat tgcccaaggt agtattgact accgtggtca ggatttgaca gctttatctt
    17281 ctcacaagga ttgggaacaa attcgtggtg ctaagattgc gactatcttc caggacccaa
    17341 tgactagttt ggaccccatt aaaacaattg gtagtcagat tacagaagtt attgtaaaac
    17401 accaaggaaa aacagctaaa gaagcgaaag aattggccat tgactacatg aataaggttg
    17461 gcattccaga cgcagataga cgttttaatg aatacccatt ccaatattct ggaggaatgc
    17521 gtcaacgtat cgttattgcg attgcccttg cctgccgacc tgatgtcttg atctgtgatg
    17581 agccaacaac tgccttggat gtaactattc aagctcagat tattgatttg ctaaaatctt
    17641 tacaaaacga gtatcatttc acaacaatct ttattaccca cgaccttggt gtggtggcaa
    17701 gtattgcgga taaggtagcg gttatgtatg caggagaaat cgttgagtat ggaacggttg
    17761 aggaagtctt ctatgaccct tgccatccat atacatggag tctcttgtct agcttgcctc
    17821 agcttgctga tgataaaggg gatctttact caatcccagg aacacctccg tcactttata
    17881 ctgacctgaa aggggatgct tttgccttgc gttctgacta cgcaatgcag attgacttcg
    17941 aacaaaaagc tcctcaattc tcagtatcag agacacattg ggctaaaact tggcttcttc
    18001 atgaggatgc tccaaaagta gaaaaaccag ctgtgattgc aaatctccat gataagatcc
    18061 gtgaaaaaat gggatttgcc catctggctg actaggagga aggaaatgtc tgaaaaatta
    18121 gtagaaatca aagatttaga aatttccttc ggtgaaggaa gtaagaagtt tgtcgcggtt
    18181 aaaaatgcta acttctttat caacaaggga gaaactttct cgcttgtagg tgagtccggt
    18241 agtgggaaaa caactattgg tcgtgctatc atcggtctaa atgatacaag taatggagat
    18301 atcatttttg atggtcaaaa gattaatggt aagaaatcgc gtgaacaagc tgcggaattg
    18361 attcgtcgaa tccagatgat tttccaagac cctgccgcaa gtttgaatga acgtgcgact
    18421 gttgattata ttatttctga aggtctttac aatcaccgtt tatttaagga tgaagaagaa
    18481 cgtaaagaga aagttcaaag tattatccgt gaagtaggtc ttcttgctga gcacttgact
    18541 cgttaccctc atgaattctc aggcggtcaa cgtcaacgta tcggtattgc ccgtgccttg
    18601 gtcatgcaac cagactttgt tattgcagat gagccaattt cagccttgga cgtttctgta
    18661 cgtgcccaag tcttgaactt gctcaaaaaa ttccaaaaag agctcggctt gacctatctc
    18721 ttcatcgccc atgacttgtc ggttgttcgc tttatttcag atcgtatcgc agttatttac
    18781 aagggtgtta ttgtagaggt tgcagaaaca gaagaattgt ttaacaatcc aattcaccca
    18841 tatactcaag ccttgctttc agcggtacca atcccagatc caatcttgga acgtaagaag
    18901 gtcttgaagg tttacgaccc aagtcaacac gactatgaga ctgataagcc atctatggta
    18961 gaaatccgtc caggtcacta tgtttgggcg aaccaagccg aattggcacg ttatcaaaaa
    19021 ggactaaact aataatggtt ttataatttc catgtcaatt tttatggaaa ttataaaact
    19081 ttttttgttg gactgatttg aaaaactttg aaagcttcct gaaagaattt tcgaaaaatt
    19141 ttaaaaaatt ctgaaatatt attgacaggt gatgaaaaag gtggtaggat aggaagcatc
    19201 aaaaaagaaa gcagaaaaag aaatgaataa gagacaagcc gtaactatga atcaaaactt
    19261 ttactttagc tactttagat agtgtttgta gacatgtcac catggatgca aacagttcaa
    19321 gcaatttgtt tgtatctatg tggctaagat ttttgattgt ggtccatata gatgaatatc
    19381 taaatggact agctaagttg taacttgtta ggttatttca agtatccgtt tagatagtat
    19441 ctaggcggtt ttttgtttta tcttttctga gaaggagttt cattatgaaa gttgttcgaa
    19501 aatcactagc cccctcttgg tagtggggct cctcttgact tctctgatca gtttgcatca
    19561 gttgaaggca gataagaaaa agatgtcttt cgtatcggta tttcgcaatt tattacccac
    19621 cagtccttat acgctactag agaaggattt gtggatgagc tggccaaaca aggctatgtt
    19681 gagggggaaa atattgagat tgatttgcaa aatgcacagg gagaacaaag aaatctaaaa
    19741 acgatttccc agcaactagc agaatccagc gatgtcgttc tagccatcgc aaggccttct
    19801 gctcagagct tggccaatac gacgcaaacg acaccagtta tcttttcagc tgtaacagac
    19861 ccagtcagtg ccaagttggt tgagtcaaga gaacatcctg ggggcaatgt aactgggaca
    19921 agcgatcagt catcagatgc catttcaacc caaatcaatt tgataaagaa agtgttgcta
    19981 aaggctaaaa cgattggaat cctctatact cagagcgagc ccaattcggt tgtttaaaag
    20041 gatgaagcta agcgacttct gaaagaaaaa ggctttaccg ttgttgaaaa aacaatcttg
    20101 gacagtaata acgtcaaggc ggcagcagag agtttgatgg cagaggtgga tatggttttt
    20161 gtcccaactg ataatatcat tttgtcaacc atggaaacag ttaagcaggt ttctattaaa
    20221 cacaaggttc cagtattcgg tggttcaaca gaaatgattg ctgttggtgg cttgtataac
    20281 tacggaacca attatgaaga attgggaaga cagacagctc gcatgctgat tcgtgtttta
    20341 aaaggtgagg aaccagaaaa tatagcagtt gagttgcctg aaaaactgga attgcatacc
    20401 aatcaagaaa tggcagatgc attgggaatt gatattagta agttagaagg caaggaataa
    20461 ggaggattaa gatgaatttt gtgttatcta gtttatcaga aggtttgttg tggtcgattg
    20521 tggcgattgg ggactacttg actttccgta ttttggatat tgcggatatg actgctgagg
    20581 gagcctttcc tctgggggca gcagtcgttg tatcacagat acaggcaggg gcaaatcctt
    20641 ggcttgcgac tttacttgct ttgctggcag gtatggtagc aggtcttgta tcaggaatgc
    20701 tccacaccaa gatgaaaatc ccagctctct tgacagggat tgtgaccttg acagggctct
    20761 attcaatcaa tattaaaatc atgggcagtg tacccaatct ttccttggga gattctgcaa
    20821 ccgtctttaa acagttggcg agcttgggtc tgacaaatga aggagctgtt ttctcactta
    20881 gtttggtctg ttttttactt gtttgtttgg tcttgaccct tttgatgaaa acagagattg
    20941 gcttggtctt gcgttcgact ggggataata tcccgatgag tgaggccaat ggagtcaatg
    21001 tagacaccat gaagattgtt ggttacatga tttcaaacgg tttgattgct ctatgtggtt
    21061 ccttgtttgc ccaaaatgat ggattttcag atgtgacttc tgggacagga accattgttg
    21121 ttggtttgag ctcggtgatt attgcggaag tcttgataca cgacttgacc attggaggcc
    21181 gcttattatc catcggaatc ggtgctattg tttaccgttt gattatttta aatatctatg
    21241 aaattccaaa tctagatcaa aacttggtac gtctctttaa tgcaatcttg ctcactttgg
    21301 ttttatttgc accagaattg caaaagagat taaagattcg tggtctgaaa ttgagaaatg
    21361 aataggagga gaaagttatg gcaagtttgt taacacttga aaatattcac aagacatttg
    21421 aagcagggac ggtcaatgag aaccatgtcc tcaaaggatt agatttagag gttgaagagg
    21481 gagattttat ctctgtgatt ggtggaaatg gagcaggaaa atccactttg atgaatatct
    21541 tggctggcaa tctatcggtg gacgaagggg accttctact ggcagggaaa tccattaaaa
    21601 atctgagtgt tagaaagaga gccaaggata ttgcccgtgt ttttcaagat cctaagatgg
    21661 gaacagcttc tagtttgacg attgaggaga atatggctat tgccttgaga cgtgggcaaa
    21721 agagagggct tggttggggc gtgaaggaga aggatagaat ccagttccaa gaggccttga
    21781 aggagttgaa tattggtctt gaaaaccgct taaaagtaga tactcaatat ctctcaggag
    21841 aacaaagaca ggctttgacc ctagtcatgg cagctctggt gaaacccaaa cttttgcttt
    21901 tggatgaaca tactgcggca cttgacccga aaaccagtca aatggtgatg gacttgacgc
    21961 aaaagattgt ggagcaccat cagtggacga ctttgatgat tacccatgat atgaatcatg
    22021 cgattgagta cggcaatcgt ctcattatgc tctatcaagg aaagatagtg gtagatgtca
    22081 agggagaaga gaaaaagcat ctgacagttg aagaccttat gcatctcttc cagaaaaata
    22141 gtggccaaag tctggtcagt gatgaattag ttttgggata aagaaaaagg ctgagatctg
    22201 gtgtctcagt cttttatttc ttgcgttttg caaaatctac aaatctaaat ttctctaact
    22261 tatggcgact ttcggtaaac tgaaattggc gtccgtcttg gagatagact tttgatttaa
    22321 tcgaaacgac ataagggtct ttgccaatgt ccatgagaat cttgtctcgg tcgcttgagt
    22381 ggtcaatggt gatttccttc tgagcataat caataaggag tttgaggcca ttttctatat
    22441 aagaatagat agactgctca gcaatttggc gagtgagatt tggaattagt tccatatcca
    22501 gatagtccgt atccagaacg gataccagat catccaccac acgctggcgg accaccttcc
    22561 aaaccatccg aaactctggg aaaccggtta tcagtgagga ttttttatca ataataatct
    22621 tgtccagact gaccacgttg gttttagagc gcagtccaag ttctttaact agttcttggt
    22681 agctggttag gttggataca gggaaattga cggtttcttc tttgacgact tgagaacctt
    22741 gccctcttat ctttttgatc aatccttcct cttggaggag agacagggcc tttcggacgg
    22801 tatcacgact cacttgatat tgctccataa ggtcgtgctc gctaggaagg aaatctccga
    22861 cagcgtaagt ctcgttttga atggtttctt ggatttgctt aaataattgt tggtatttct
    22921 tcattttgcc tgcttaaata ggtttttagt ctagtttaat gaacacctgt atctatttta
    22981 ccatacaagt gctagaaaat ttacaaaaaa acaccctttt tacaccaaac aagttgcaga
    23041 caagtttggt atcgtttaca atatacttat caaatcaaac ttgctttgac aagtataagg
    23101 agaatcaaat gggaaaattt gaacaagaag ccaaagatct gcttcaggca attggaggca
    23161 aagaaaatgt gactgccgta actcactgtg cgacacggat gcgctttgtt ttaggagatg
    23221 ataagaaggc taatgttaaa gctatcgagt caattccagc tgttaaagga acttttacaa
    23281 atgcaggtca atttcaggta atcattggaa atgacgtgcc catcttttat aatgatttta
    23341 cagccgtttc aggtattgag ggtgtttcca aagaagcagc caagtctgca gctaagagta
    23401 atcaaaacgt ggtccaacgt gttatgacca ctctggcgga gatttttact ccgattattc
    23461 cagccttgat agtcggagga ttgatcctcg gtttccgtaa tgtcttggaa ggtgtccatt
    23521 ggtcgatgtt ggatggcaag accatcacag aatcctctca gttttgggca ggggtcaatc
    23581 acttcctttg gttgcctggt gaagctatct tccagttctt accagtaggg attacttggt
    23641 ctgtttctcg taagatggga accagccaaa ttttgggaat tgttctcgga atctgtttgg
    23701 tatcgcctca gttgctcaat gcctatgcgg ttgcttcaac gccagcagct gatatcgagg
    23761 caaactgggt ttggaatttt ggttatttta ctgttaatcg tattggttac caagcccaag
    23821 ttatcccagc cttgcttgca ggtttgagtc tgtcttatct tgaaattttc tggcgcaaac
    23881 atatcccaga agtcatttcc atgatttttg tacctttttt gtcattgatt ccagccttga
    23941 ttttggctca tactgttttg ggaccaatcg gttggacaat tggacaagga ctttcatcag
    24001 ttgtcttggc aggtttaact ggtccagtta aatggctctt cggtgcaatt tttggtgccc
    24061 tctacgctcc atttgtcatc acaggtctgc accatatgac caatgccatt gatacacaat
    24121 tgattgcgga tgctggtggc actgccctat ggccaatgat tgctctttct aatattgctc
    24181 aaggctcagc cgtgtttgcc tattatttca tgcatcgcca tgatgagcgt gaggctcagg
    24241 tttcacttcc tgcaaccatt tcagcctatc tcggtgttac agaaccagct ctttttgggg
    24301 ttaacgtaaa atatatttat ccatttgttg ctgggatgac tggttcagcc cttgcaggca
    24361 tgttatccgt tacttttaat gtaactgcgg cttctattgg tatcggtggt ttgccaggta
    24421 ttctctctat tcaacctcaa tacatgctgc catttgcagg aactatgcta gttgcgattg
    24481 ttgttccaat gctcttgact ttcttcttcc gcaaggctgg tctctttaca aaaacagagg
    24541 gcgatacgaa cttgcaggca gaattcgttg ctcaagaaga agcagaattt gtgagccatg
    24601 aactagtaga acttacttcg gtagaaatta tcagcccact aactggccaa gtgaaagaat
    24661 tgagtcaagc gacggatcct gtttttgcat caggtgtcat ggggcaaggt ctggtcattg
    24721 aaccaagcca aggtgagttg acctctccag ttaatggaac agtgacggtt cttttcccta
    24781 ccaagcatgc catcggcatt gtctctgacg agggagttga attgctcatc cacatcggta
    24841 tggatacagt aggtcttgat ggcaaaggtt ttgaaagtct tgtagtccaa ggagatcacg
    24901 ttacagttgg tcagcaactg attcgttttg atatggatgt cattaaggct gcaggtctgg
    24961 tgacagaaac tcctgttatc atcaccaacc aagatgctta tacagcgact attactggaa
    25021 cttatccgac aacgatccaa gctggagcat ctctcatggt cgctacacga atctaatact
    25081 cttcgaaaat ctcttcaaac cacgtcagct ctatctgcaa cctcaaaata gtgttttgag
    25141 caacctgtgg ctagcttcct agtttgctct ttgattttca ttgagtataa ttgaaatagc
    25201 acaaataggg acggaagact gcttcctccc ttttatcaga aaaggagaaa cagatgacac
    25261 ttgataaagg aaaagtagtc tatcaaatct atccaaaatc ttacaaagac accactgaaa
    25321 acggtattgg ggatttccgt gggattatcg aaaaaattcc ctatttagcc aaactaggtg
    25381 tggatatggt ctggctcaat ccattctatc caagccctca acgggataat ggttacgata
    25441 tttcagatta tatggcagtg gatcctcttt ttggtgatat ggctgatttt gaggaaatgg
    25501 tatgtgtcgg taaagagcac aagattgact ttatgctgga catggtactt aatcattgtt
    25561 cgacagagca tgaatggttt cagaaagccc tagctggtga taagtattat caagactttt
    25621 tcttcatcca agaccaacca acagattggc agtctaagtt tggtggttct gcatgggcgc
    25681 ctttcgggga tactgggaaa tactatcttc acctatttga tgagacccag gctgacctta
    25741 actggcgcaa tcccaatgtc cgtaaggagc ttttcaaggt tgttaatttc tggcgcgaca
    25801 agggtgtcaa aggttttcga tttgatgtga tcaatttgat cggtaaggac gaggtttcag
    25861 tggactgtcc tgaaaatgaa gggaagccag cttatacaga caaacccatc gttcataact
    25921 atttgcgtat gatgaaccaa gccacttttg gatctgacga tagctttatg acagttgggg
    25981 aaatgtcttc taccaccatg gaaaactgtg tcctttattc gtcgcctgat cgtcaggaat
    26041 tatccatgac ctttaatttt catcacctca aggtggacta taaggatgga caaaagtgga
    26101 ccttggctcc ctttgatttt gaagagttga agagtcttta ccatagttgg ggtaaggaaa
    26161 tgagtgataa agacggttgg agtgccctct tttggaacaa tcacgatcaa ccacgtgcct
    26221 tgaaccgttt tgtcgatatt caaaacttcc gcaaggaagg agccaccatg ctagcagcca
    26281 gcattcacct gtcacgtggg acaccttata tctacatggg tgaggaaatc gggatgattg
    26341 acccagacta tgattctatg gctgattatg tggatgtcga atcgcttaat gcctatcaga
    26401 tgctcttaga agaaggaaag agtcagcaag aagccttcca gattattcaa gctaagtcgc
    26461 gtgataattc acgcattccc atgcagtggg atgcttcgga aaatgcagga ttttcaacag
    26521 gcactccttg gttgaaagct ggtaaatcct ataaatacat caatgtagaa aatgaaatcc
    26581 agggtccgat ttttaccttc tatcaagact tgattcgact gcgtaaggaa atgccaatca
    26641 tttcagaagg aagttacaaa ccagcctttg aagatagcaa acaagtctac gcttttgaac
    26701 gccagtttga ggatcaaaag ttactagtgc tcaataactt ttatgctaaa gaagtggaaa
    26761 tcgacttgcc agcagtctac caaaatggac agattttgat atcaaactat gaagatgcag
    26821 aagtgtctga gaaaattcta ctcaaaccct atcaaacact ggctatttat gtaaattaat
    26881 actcttcgaa aatctcttca aaccgtgtca gttttatctg caacctcaaa gcggtgcttt
    26941 gagcaacctg tggctagctt cctagtttgc tctttgattt tcattgagta taaacttagc
    27001 aaggagacct ctttttcaga aaagcagggt tctttttgct atctattcca tccctgaaag
    27061 tgtagaacaa actggtcagg gtggctagtt tgtggtataa tgaaagggat tcaaaaagaa
    27121 acaggagaaa aatgatggat ttacttttag caattgtatt gattgtgcta gcttttctag
    27181 gaggagctct tggaggaatg tacttggttc gtaagcaaat cgaaaaagaa tttgctgaca
    27241 acccacgttt gaatgctgaa gcagttcgta ctcttttgag tgcaaatggt caaaaaccaa
    27301 gcgaagctaa ggtacaacaa gtttaccacc aaatcatccg ccaacaaaag gcagcccttg
    27361 ctaacaataa aaagaaaaaa taaataagga aaagtctggg atgaaagttc cagacttctc
    27421 actatgttgg ttatggttta gggatgagac tttttccttg cattctattg atatttgatt
    27481 atgattaaga gagaggtagt tgacattcta tcaagctgtc ggtcagttcg ttcagtacaa
    27541 ggaatcataa tgaacgatca atcagaaaaa agactagaaa gaagactgta tggataatcg
    27601 accaattggt tttttggatt cgggtgtcgg gggcttgacc gttgtgcgcg agctcatgcg
    27661 ccagcttccc catgaagaaa tcgtctatat tggagattcg gcgcgggcgc cctatggccc
    27721 ccgtcctgct gagcaaattc gtgaatatac ttggcagctg gtcaactttc tcttgaccaa
    27781 ggatgtcaaa atgattgtca ttgcttgtaa cactgcgact gcggtcgtct gggaagaaat
    27841 caaggctcaa ctagatattc ctgtcttggg tgtaattttg ccaggagctt cggcagccat
    27901 caagtccagt caaggtggga aaatcggagt gattggaacg cccatgacgg tacaatcaga
    27961 catataccgt cagaaaatcc atgatctgga tcccgactta caggtggaga gcttggcctg
    28021 tcccaagttt gctcccttgg ttgagtcagg tgccctgtca accagtgtta ccaagaaggt
    28081 ggtctatgaa accctgcgtc ccttggttgg aaaggtggat agcctgattt tgggctgtac
    28141 tcattatcca ctccttcgcc ctattatcca aaatgtgatg gggccaaagg ttcagctcat
    28201 cgatagtggg gcagagtgcg tacgggatat ctcagtctta ctcaattatt ttgaaatcaa
    28261 tcgtggtcgc gatgctggac cactccatca ccgtttttac acaacagcca gtagccaaag
    28321 ttttgcacaa attggtgaag aatggctgga aaaagagatt catgtggagc atgtagaatt
    28381 atgacaaata aaatttatga atataaggat gaccaggact ggtatgttgg gtcttatagt
    28441 atttttggtg gcgttaacag tttgagcgac tataagacag attttcctct gtttgaattc
    28501 tccaaaatat ttggagatga agagtatggt ttcccgcttt cagttactgt tttacgctat
    28561 ggttctacct accgtttgtt ctcctttgtg gtagacatgc ttaatcaaga aatgggacga
    28621 aacttggaag ttattcaacg tcatggggcc ctgctcttgg ttgaaaatgg gcaactcttg
    28681 tatgtagaat tgcctaaaga aggggtcaat gttcatgatt tctttgagac aagcaaggtc
    28741 agagaaacct tgttgattgc gactcgtaac gaaggtaaaa ccaaggaatt ccgagctatc
    28801 tttgataagt taggctacga tgtggaaaat cttaatgact accctgacct gcctgaagta
    28861 gcagaaacag gtatgacctt tgaagaaaat gcccgcctta aggcagaaac catttctcaa
    28921 ttaacgggca agatggtttt ggcagatgat tctggtctca aagtcgatgt ccttggtggc
    28981 ttaccaggcg tctggtcagc tcgtttcgca ggtgtgggag caactgaccg tgaaaataat
    29041 gccaaactct tgcacgaatt ggccatggtc tttgaactca aggaccgctc ggctcagttc
    29101 cacacaaccc tagtcgtagc cagcccaaat aaggaaagtt tagttgttga agcagactgg
    29161 tcaggttata ttaactttga acctaagggt gaaaatggct ttggctatga tcccctcttc
    29221 cttgtaggag aaacaggtga gtcatcagct gaattaaccc tggaagaaaa aaatagtcaa
    29281 tctcaccgtg ccttagccgt taagaaactt ttggaggtat ttccatcatg gcaaagcaaa
    29341 ccatcattgt aatgagcgat tcccatggcg atagcttgat tgtggaagaa gtccgtgatc
    29401 gctatgtggg caaagtcgat gccgtttttc ataacggcga ttctgaacta cgtccggatt
    29461 ctccactttg ggagggcatc cgcgttgtta aagggaacat ggacttctac gccggctacc
    29521 cagaacgtct ggtgactgag cttggttcga ccaagattat ccaaactcat ggtcacttgt
    29581 ttgacatcaa tttcaacttt caaaagttgg actactgggc tcaggaggaa gaggccgcta
    29641 tctgcctcta tggtcacttg catgtgccaa gtgcttggtt ggaaggcaag atcctctttc
    29701 taaatccagg ctctatcagt caaccacgag gtaccatcag agaatgtctc tatgctcgtg
    29761 tggagattga tgatagttac ttcaaagtgg actttttgac acgagatcac gaggtgtatc
    29821 caggtttgtc caaggagttt agccgatgat tgccaaggag tttgagactt tcttgttggg
    29881 gcaggaggaa acttttttga cccctgctaa aaatctagct gtgttgattg atacccacaa
    29941 tgcggatcat gcgaccctct tgctcagtca gatgacctat acccgtgttc ccgttgtgac
    30001 agatgaaaaa cagtttgttg ggacgattgg actcagagat attatggctt atcagatgga
    30061 gcatgacttg agccaagaaa tcatggcgga tacggatatc gttcatatga caaaaacgga
    30121 cgtagcggtt gtttcgcctg atttcaccat tacggaggtc ttgcacaagc tagtagatga
    30181 gtccttctta ccggttgtgg atgcagaggg tattttccaa gggattatta cgcgcaagtc
    30241 catcctcaag gccgttaatg ccctcttgca tgactttagt aaggaatatg agattcgatg
    30301 ccaatgagag acaggatttc agtcttttta gaggaaaagc agggcttgtc tgtcaattcc
    30361 aagcagtcct ataagtatga tttggagcaa tttttagaca tggtaggtga gcggatttct
    30421 gagaccagtc tcaagattta ccaagcccag ctagccaatc taaaaatcag cgcccagaag
    30481 cgaaagattt cggcctgtaa ccaatttcta tactttctct atcaaaaagg agaggtggac
    30541 agcttttacc gcttggaatt agccaaacaa gctgaaaaga agacggaaaa gccagagatt
    30601 ctatacctag actctttttg gcaggaaagc gaccatccag agggccgctt gctagcgctc
    30661 ttaatcctag aaatggggct cttgcccagt gagattttag ccatcaaggt tgcggacatc
    30721 aatctggatt ttcaggtgtt gcgaatcagc aaggcttccc aacagaggat tgtcaccatt
    30781 cccacggcct tgctttcaga attggaaccc ttgatggggc agacctatct ttttgaaaga
    30841 ggagagaaac cctattctcg tcagtgggcc tttcgtcagt tagaatcttt tgtcaaggag
    30901 aaaggttttc catccttatc agctcaagtc ttacgtgaac agtttattct aagacaaata
    30961 gaaaacaagg tcgatttgta cgaaattgca aaaaaattag gattaaaaac agtcctgacc
    31021 ttagaaaaat atagataatg gatattaaat taaaagattt tgaaggaccc ctggacttgc
    31081 tcttgcatct ggtttctaag taccagatgg atatctacga tgtgcccatt acggaagtca
    31141 tcgaacagta tctagcctat gtctcaaccc tgcaggccat gcgtctggaa gtgacgggtg
    31201 agtacatggt catggctagt cagctcatgc tgattaagag tcgtaaactc cttccgaagg
    31261 tagcagaagt gacagacttg ggggatgacc tggagcagga cctcctctct caaatcgaag
    31321 aatatcgcaa gttcaagctc ttgggtgagc acttggaagc caagcaccaa gaacgggccc
    31381 agtattattc caaagcgccg acagagttga tttacgaaga tgcggagctt gtgcatgaca
    31441 agacgaccat tgacctcttt ttggcttttt caaatatcct agccaagaaa aaagaggagt
    31501 ttgcacaaaa tcacacgacg atcttgcggg atgagtataa gattgaggac atgatgatta
    31561 tcgtgaaaga gtccttggtt ggacgagctc aattgcgctt gcaggatttg ttcaaggaag
    31621 cccagaatgt ccaagaggtc atcaccctct ttttggcaac cctagagtta atcaaaaccc
    31681 aggagttgat cctcgtgcaa gaggagagtt ttggagatat ctatctcatg gaaaagaagg
    31741 aagaaagtca agtgcctcaa agctagactt aatagagagg aaagatgagt actttagcaa
    31801 aaatagaagc gctcttgttt gtagcgggtg aagatgggat tcgggtccgc cagttagctg
    31861 aactcctctc tctgccaccg acaggcatcc agcaaagttt aggaaaatta gcccagaagt
    31921 atgaaaagga cccagattcc agtttggctt tgattgagac aagtggtgct tatagattgg
    31981 tgaccaagcc tcaatttgca gagattttga aggaatactc taaggcgcct atcaaccaga
    32041 gcttgtctcg ggctgccctt gagaccttgt ccattattgc ctacaaacag ccgattacgc
    32101 ggatagaaat tgatgccatc cgtggagtta actcgagtgg agccttggca aagttgcagg
    32161 cttttgacct gataaaggaa gacgggaaaa aggaagtatt ggggcgcccc aacctctatg
    32221 tgactacgga ttatttccta gattacatgg ggataaacca tttagaagaa ttaccagtga
    32281 ttgatgagct tgagattcaa gcccaagaaa gccaattatt tggtgaaagg atagaagaag
    32341 atgagaatca ataagtatat tgcccacgca ggtgtggcca gtaggagaaa agcagaagag
    32401 ctgattaagc aaggcttggt gacggttaac ggccaagtgg tgcgtgaact agcaaccact
    32461 atcaagtcag gcgacaaggt cgaagttgaa ggtcaaccta tctacaacga agaaaaggtc
    32521 tactatctgc ttaacaaacc acgcggtgtg atttccagtg tgacagatga taagggtcgc
    32581 aagacggttg tcgacctctt gcccaatgtc aaagagcgta tttaccctgt gggtcgtttg
    32641 gactgggata catcaggtgt cttgattttg accaatgatg gggactttac agacgagatg
    32701 attcaccctc gtaatgagat tgacaaggtt tatgtcgcgc gtgttaaagg tgtggccaat
    32761 aaggacaatc tccgcccctt gacccgtggt cttgagattg atggtaagaa aaccaagcca
    32821 gctgtttatg aaattctcaa agtggaccca gtcaaaaatc gctctgtggt gcagttgacc
    32881 atccatgaag ggcgtaacca tcaggttaaa aagatgtttg aagctgttgg tctccaagta
    32941 gataagttgt ctcggactcg tttcggacac ctagacttga caggactccg tccaggagaa
    33001 tcccgtcgtc ttaataaaaa agaaatcagc caactacaca ccatggctgt aactaagaaa
    33061 taatgaaacg aattttaata gcgcctgtgc gcttttacca acgttttatc tcaccagtct
    33121 ttccaccctc ttgtcgcttt gagctgactt gttccaacta catgattcag gctattgaaa
    33181 aacatggttt taagggggta ttgatgggct tggctcggat tttacgttgt catccctggt
    33241 cgaaaacagg taaggatccc gttccagacc acttttccct taaacgaaat caagaagggg
    33301 aatgaggtgg ggtaaataga tttcaaaatg ataaaaacgc atcctatcag gtttgagtaa
    33361 acttgatagg atgcgtttta gaatgtcaaa attttatact cttcgaaaat ctcttcaaac
    33421 cgcgtcagct ttcatctgca acctcaaaac agtgttttga gcaacctgcg gctagtttcc
    33481 tagtttgctc tttgattttc attgagtatt aaattgagtt tgaagtggct tatttcaaag
    33541 ctttttgtat gtcttcaatc atgagttttg ttgattcaag tccgcctccg cttagatacc
    33601 agaggtctgg tgttagttgg ataatcttac catttttagc agcaggtgtt tcagcgataa
    33661 gggcattttc taggacaccg tcgttgctag agttgtcccc accgatggca agggtacggt
    33721 tgatgacaaa gaggatgtca gggttgattt ctttgacact ttcaaagctg acttcttgtc
    33781 cgtggcgtga gtcttcaaat tttgtatcag ttggtttgaa tttcaaggtt tggtacaaga
    33841 aagagaaacg agatttggca ccaaaggctg ccatttttcc ttcattaagg aggatcgcaa
    33901 gggctttttt gtcagagctt tcatttttag tagcgacttc ttggatgctc ttgtctagct
    33961 tggccaattc ttccttggct ttctgtgtac cagtttcgcc gaaggcactt gctaaggatt
    34021 cgatattagc cttggtagaa gtccagtagt catccttgct tgcttggaag agaacggttg
    34081 gggcgatttc tttgaatttg tctacgaatt tttgtgtacg tggtgaagcg ataatcaaat
    34141 caggctcaag ggcggcgata gcttctaaat caggttcttt catagaacca acatttttga
    34201 cagttcccac taggtctttt agataagtcg gaacagtttt tgtaggcatt ccgacgatat
    34261 ttttttcaaa tcctaaagcg cgaatagtat ccgcagcgcc gaggtcaaag gtcacaatct
    34321 tttcaggaac tttggaaagt ttgacctcgt ccagtgaact tttaatggtt atctctgttg
    34381 gagcagagct actggtctct gtctgactag tgcttgagtt tgtactacat gcaccaagta
    34441 ggagcaagaa gctggccact agggcagtga aataaagttt aagggatgtt ttcataattt
    34501 ctccttttta aaatgtgata acgatttagg gagtctctta atcttattga ctaagagact
    34561 gaaggttctc taacttgagc ttttatgtta ctagctatag atacagatct ttttgtcatt
    34621 gatatcagct agcgtgatgg gaatctcata aagttgactg agcaggtcag cctgcatgat
    34681 ttgatcggtt cttcccttgc taaagacctg gccgtccttg aaggcgacaa tttcatctgc
    34741 atactgactg gccatgttga tatcgtggag gacgatgata atggtcttgc cgagttcctc
    34801 caccagtcgt cgaagaatct gcatcatgct gacgctttgc ttgatatcga gattgttgag
    34861 tggttcgtcc agcaagataa agtccgtatc ctgggccagt accatagcga taaagacgcg
    34921 ctggagttgc ccccctgaca ggctattgat gtagcggtct tttaagttgg tcagttccaa
    34981 atagttcaga gtttctcgga ttttttccca gtcttctgat ctaagtcgac ctcggctgta
    35041 gggaaaacgt ccaaaactga ccagttcttc aacagtcaat ttggcttggt aattgatttt
    35101 ctgttttagg atggttagtt cttgggccag ttcttgcgaa ttccagctct cgatttcacg
    35161 tcctttgata ctgagaactc cctgatcttt cttggttagt ctgctcatga tggagaggag
    35221 agtcgatttt ccagcaccat ttggaccaat aaaggctgtc agtttttgag gactgacttc
    35281 aagcgaaatg ccttgcaaaa tatcctgttt ttgaatggat ttgtcaatgt tttccagttt
    35341 cactgacgag ccctcctata tagtaagata aagaataaga agccacccac actctcaatg
    35401 atcatactga tacgaatttc cagtgcaaag actcgttcaa tcaaggcttg ccccaaggtt
    35461 aagctaataa atccaaccag aatggccact ataaagagta acttgtgctg atagtctttg
    35521 acaatcaggt aggtgaggtt ggccagcata aagccgaaga aggccatagg tcctaccaag
    35581 gcagtggccg ttgaggtcaa aagcacgatt ccccagagga gctctttctg ttctttttca
    35641 acatcgagtc ccaatatctg agccgtttct ctttgcaggt gcaagacatc tagaacgact
    35701 gcttttcgaa agaaaaagat tgtcaaagcg aggatgatca gagaaccgat ggctaggatg
    35761 gaagtgttaa gatgttgaaa ggaggcaaaa agactatttt gcagtttatc gtattcgttt
    35821 ggatccatta ggacttgaag gaaggtgctg atatttcgaa agagacttct gagcgctaga
    35881 cagatcagca ggatgaagac caggtcttgc ttcatcagtg tcttcaagta accttgtaag
    35941 gcgagaaaga agaaggactg gacaagaagt aagactagga attctaagat aggggatttg
    36001 ccaagttgaa gaaacttgct ttcaaaaacc agtagtaggg tttgtagtag gacgtagaag
    36061 gattcaattc ccaaaatact aggcgtcagg aagcgatttt ccgtcagggt ttgaaaacta
    36121 atggtcgaaa tcccagtcgc gatggctacc aagagataaa cgatgatctt ttgggaacgc
    36181 aacttccaag caaaggctga caagtgagtg atgggccaaa agtagagaag acaagctccg
    36241 atagcaagaa taatgagaat ccagaagagc ttggtatgtt tgcttttagt ctgcatcttt
    36301 tcgtccccct ctccagagaa gtaggataaa gacgagacta ccgatgattc ctagcaagag
    36361 actgacagac aactcatagg gcctaatcag aactcgggat aggatatcgc aagccagaac
    36421 tagattggca ccaaccagtg cgaccatgag tttggtttga cttagattat ctccatagcg
    36481 cttgcgaaca agattgggaa cgataactcc gagaaatggt aagccaccca cggtaatcat
    36541 ggtgacgctt gtcgttagcg ccaccagaaa gagggccagt ttttcaagta gggagtagga
    36601 aatccccaaa ctctcgctgg tttctttccc cagattcatg atggtgaagg tttgggataa
    36661 tttccaaacg gttatcagga tgatgaggcc taagaagagc cactcatact gatgggtctg
    36721 aatcatggag aaggagccct gggtccaggc agtcatactc tgaaccagat tgaaacgata
    36781 ggcgataact tctgtgactg agccgataat cccgctatag atgatcccaa tcagaggcaa
    36841 catccacctt tcctttacag taaaaatggt cataaaggct aggaagaaga gggtgaatac
    36901 gatggatgaa acaaaagcga agagcatctt ttgggtcaga ctagccgatg gaaagacaaa
    36961 aaggctcagc accattccca gtttggcggc ttcagtcgtt ccaactgtac tcggtgcagc
    37021 aaactgattt tgggtaatag tctgcatgag aaggcctgcc atactcatac tagaggcagt
    37081 caggagaata ctgatagttc ttgggagacg ggactcttga aagaggagcc aggtctgctg
    37141 gtcgaaatca aatagctttc cccatgaaaa atcactggtc ccaatgctaa tagagagaaa
    37201 gactaggagt agaagtaagc caattaaata atgagaaagt ttcatacccc gtcctttcat
    37261 gtagatttgg tatcgaaaga tatctgcgga tataaatgta acattatttt tctaatctgt
    37321 caataaaatt tctgacaatt taataaatac aacaaggaga gagcaacaag actttctcct
    37381 ttgttatcct attctaaaat gtttttacct taatctgata aaataatatc ttcgagggag
    37441 tagctagccg tccaatcaag atattgttta gcttttgaag catctgctag gacactggct
    37501 gggtcactag cacgtcgagc aacaatctcg tgtgggattt tttaatttag taattcttca
    37561 gcagttttaa agatttcttt gatagtatag ccttttttag ttcctaagtt aaagatttga
    37621 gaagaactgt cttcttgaaa taggtagttc attcctttaa catgagccta tgcaaggtcc
    37681 aagacataaa tgtaatctcg aatacatgaa ccgtcacgtg tatcgtagtc atctccaaat
    37741 atttttaagc tatcatgttg tcccaatgcg gtcttgttga tatttggaat gatgtgagtt
    37801 ggatttttca cacgcagacc gtttgaagca tccatttcag ccccagcaac attaaagtaa
    37861 cggaaaataa catatttcca gtcgtagcga ttggccatcc agtaaatcat tcgttcgccc
    37921 atcagttttg tctctgcata agggttgaca gggtcgagca gggtatcttc agtcatcggc
    37981 ttgtcaatac agttatttcc atagagagaa gcagtcgaag agaacatgat tttttgaatg
    38041 ccaacttcag ataagacttt gagaacttgg ttcataccag caacgttggc agtgaagtat
    38101 ttacttggat tttcaatact ttcgctcaca acaatttcac ctgcacaatg gaggacagca
    38161 tcaatctgat tttcttctaa gtaagccttt aaggcgctag catcataaac atccagttct
    38221 ttaaagctag cacgactatc cacagccgct cgatttcctg tggagagatt atcgaggaca
    38281 tgcacctgat agccagcatt taaaagagct ttaacggtat gggagccaat gtagctagtc
    38341 ccacctgtaa caagaattgt tttggtcatg attttttcct tttctagttt tgtactatag
    38401 ta
//
LOCUS       DAVLVP010000009        37860 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.009, whole genome shotgun sequence.
ACCESSION   DAVLVP010000009 DAVLVP010000000
VERSION     DAVLVP010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 37860)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37860)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37860
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.009"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..170
                     /locus_tag="VYN69_000453"
     CDS             <1..170
                     /locus_tag="VYN69_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="choline-binding protein"
                     /protein_id="HEV1532783.1"
                     /translation="QYNGSWYYLNSNGAMKASQWFKVSDKWYYVNGSGALAVNTTVDS
                     YRVNANGEWVN"
     gene            complement(400..1380)
                     /gene="dusB"
                     /locus_tag="VYN69_000454"
     CDS             complement(400..1380)
                     /gene="dusB"
                     /locus_tag="VYN69_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEV1532784.1"
                     /translation="MTNLNTPFMIGNVEIPNRTVLAPMAGVTNSAFRTIAKELGAGLV
                     VMEMVSDKGIQYNNEKTLHMLHIDEGENPVSIQLFGSDEDSLARAAEFIQENTKTDIV
                     DINMGCPVNKIVKNEAGAMWLKDPDKIYSIINKVQSVLDIPLTVKMRTGWADPSLAVE
                     NALAAEAAGVSALAMHGRTREQMYTGHADLETLYKVAQALTKIPFIANGDIRTVQEAK
                     QRIEEVGADAVMIGRAAMGNPYLFNQINHYFETGEILPDLTFEDKMKIAYEHLKRLIN
                     LKGENVAVREFRGLAPHYLRGTSGAAKLRGAISQASTLAEIEALLQLEKA"
     gene            complement(1367..2239)
                     /gene="hslO"
                     /locus_tag="VYN69_000455"
     CDS             complement(1367..2239)
                     /gene="hslO"
                     /locus_tag="VYN69_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEV1532785.1"
                     /translation="MDKIIKTISESGAFRAFVLDSTETVRTAQEKHQTQASSTVALGR
                     TLIASQILAANEKGNTKLTVKVLGSSSLGAIITVADTKGNVKGYVQNPGVDIKKTATG
                     EVLVGPFVGNGQFLVITDYGAGNPYNSITPLISGEIGEDLAFYLTESQQTPSAVGLNV
                     LLDEEDKVKVAGGFLVQVLPGAKKEEIARFEKRIQEMPAISTLLESDDHIEALLKAIY
                     GDEAYKRLSEEEIRFQCDCSHERFMNALASLPSSDLQEMKEEDHGAEITCQFCQTTYN
                     FDEKDLEELIRDKS"
     gene            2404..3831
                     /locus_tag="VYN69_000456"
     CDS             2404..3831
                     /locus_tag="VYN69_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1532786.1"
                     /translation="MYLGDLMEKAECGQFSILSFLLQESQTTVKAVMEETGFSKATLT
                     KYVTLLNDKALDSGLELAIHSEDENLRLSIGAATKGRDIRSLFLESAVKYQILVYLLY
                     YQQFLAHQLAQELVISEATLGRHLAGLNQILSEFDLSIQNGRWRGPEHQIRYFYFCLF
                     RKVWSSQEWEGHMQKPERKQEIANLEEICGASLSVGQKLGLVLWAHISQQRLRVNACQ
                     FQVIEEKMRGYFDNIFYLRLLRKVPSFFAGQHIPLGVEDGEMMIFFSFLLSHRILPLH
                     TMEYILGFGGQLADLLTQLIQEMKKEELLGDYTEDHVTYELSQLCAQVYLYKGYILQD
                     RYKYQLENRHPYLLMEHDFKETAEEIFHALPTFQQGTDLDKKILWEWLQLIEYMAENG
                     GQHMRIGLDLTSGFLVFSRMAAILKRYLEYNRFITIEAYDPSRHYDLLVTNNPIHKKE
                     QPPVYYLKNDLDMEDLAGIRQLLFT"
     gene            3990..5498
                     /gene="glpK"
                     /locus_tag="VYN69_000457"
     CDS             3990..5498
                     /gene="glpK"
                     /locus_tag="VYN69_000457"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000076796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEV1532787.1"
                     /translation="MSQEKYIMAIDQGTTSSRAIIFNKKGEKVSSSQKEFTQIFPQAG
                     WVEHNANEIWNSVQSVIAGAFIESGVKPNQIEAIGITNQRETTVVWDKKTGLPIYNAI
                     VWQSRQTAPLAEQLKSQGYVEKFHEKTGLIIDAYFSATKVRWILDHVEGAQERAEKGE
                     LLFGTIDTWLVWKLTDGAAHVTDYSNAARTMLYNIKELKWDDEILEILNIPKAILPEV
                     RSNSEIYGKTAPFHFYGGEVPISGMAGDQQAALFGQLAFEPGMVKNTYGTGSFIIMNT
                     GEEMQLSENNLLTTIGYGINGKVYYALEGSIFIAGSAIQWLRDGLRMVENSPESEKYA
                     RDSHNNDEVYVVPAFTGLGAPYWNQNARGSVFGLTRGTSKEDFIKATLQSIAYQVRDI
                     IDTMQVDTQTAIQVLKVDGGAAMNNFLMQFQADILGIDIARAKNLETTALGAAFLAGL
                     SVGYWKDLDELKLLNETGELFEPSMNESRKEQLYKGWKKAVKATQVFAEVDD"
     gene            5540..7366
                     /gene="glpO"
                     /locus_tag="VYN69_000458"
     CDS             5540..7366
                     /gene="glpO"
                     /locus_tag="VYN69_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEV1532788.1"
                     /translation="MEFSKKTRELSIKKMQERTLDLLIIGGGITGAGVALQAAASGLE
                     TGLIEMQDFAEGTSSRSTKLVHGGLRYLKQFDVEVVSDTVSERAVVQQIAPHIPKPDP
                     MLLPVYDEDGATFSLFRLKVAMDLYDLLAGVSNTPTANKVLSKKEVLERQPNLKKEGL
                     VGGGVYLDFRNNDARLVIENIKRANQDGALIANHVKAEGFLFDESGKITVVVARDLLT
                     DQVFEIKARLVINTTGPWSDKVRNLSNKGTQFSQMRPTKGVHLVVDSSKIKVSQPVYF
                     DTGLGDGRMVFVLPRENKTYFGTTDTDYTGDLEHPKVTQEDVDYLLGIVNNRFPESNI
                     TIDDIESSWAGLRPLIAGNSASDYNGGNNGTISDESFDNLIATVESYLSKEKTREDVE
                     SAVSKLESSTSEKYLDPSAVSRGSSLDRDDNGLLTLAGGKITDYRKMAEGAMERVVDI
                     LKAEFDRSFKLINSKTYPVSGGELNPANVDSEIEAFAQLGVSRGLDSKEAHYLANLYG
                     SNAPKVFALAHSLEQAPGLSLADTLSLHYAMRNELALSPVDFLLRRTNHMLFMRDSLD
                     SIVEPVLDEMGRFYDWTEEEKATYRADVEAALANNDLAELKN"
     gene            7436..8140
                     /locus_tag="VYN69_000459"
     CDS             7436..8140
                     /locus_tag="VYN69_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1532789.1"
                     /translation="MMNELFGEFLGTLILILLGNGVVAGVVLPKTKSNSSGWIVITMG
                     WGIAVAVAVFVSGKLSPAHLNPAVTIGVALKGDLPWASVLPYILAQFAGAMLGQILVW
                     LQFKPHYEAEENAGNILATFSTGPAIKDTVSNLISEILGTFVLVLTIFALGLYDFQAG
                     IGTFAVGTLIVGIGLSLGGTTGYALNPARDLGPRIMHSILPISNKGDGDWSYAWIPVV
                     GPVIGAALAVLVFSLF"
     gene            8494..8640
                     /locus_tag="VYN69_000460"
     CDS             8494..8640
                     /locus_tag="VYN69_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004247184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532790.1"
                     /translation="MLVEFHKSQGTLETPEAQAEIAQKREEIEQRRAELEDKKQELLN
                     RLNK"
     gene            complement(8727..9335)
                     /locus_tag="VYN69_000461"
     CDS             complement(8727..9335)
                     /locus_tag="VYN69_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532791.1"
                     /translation="MSSISPSLVVFADDSLSANSKVVSGEAQFENGSSVRFGDTQVNI
                     LSDEVLEVVNPDGSVDTIERRADGVYINGAFYMAYQKNEIDLNISFRSYDPNVWNYVN
                     TIHGNKQANTFANFMTGAGISYMIGRIGALLGGPWGAMIGGAYFGIQAYQSYLDSQSP
                     YPYYITSTYIHVAQRKWKFITEYYRNSNYTGYVKTVTTYVNF"
     gene            10427..10930
                     /locus_tag="VYN69_000462"
                     /pseudo
     CDS             10427..10930
                     /locus_tag="VYN69_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            11009..11530
                     /locus_tag="VYN69_000463"
     CDS             11009..11530
                     /locus_tag="VYN69_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1532792.1"
                     /translation="MEIFSNTIWVSSIILVFVTELLNKTESYWGYSNTAYSIGIIISG
                     LIAFRLSEKFLAAKWESILFPLVAMAIVTLTILYFPNAQMFLLFSALVGMLSQLKEVP
                     ESVFLQETVEENHLVNVYSVLEVISTLAFSVFVLLMSYITENFGISISFWLSAICLMI
                     EAILIYIRRDYFK"
     gene            11844..11975
                     /locus_tag="VYN69_000464"
     CDS             11844..11975
                     /locus_tag="VYN69_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEV1532793.1"
                     /translation="MKQRKELYLFLGRTALYFLIFLGLLYFFSYLGQGQGSFIYNEF"
     gene            11996..13546
                     /gene="dltA"
                     /locus_tag="VYN69_000465"
     CDS             11996..13546
                     /gene="dltA"
                     /locus_tag="VYN69_000465"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012972622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEV1532794.1"
                     /translation="MSNKPIADMIETIEHFAQTQPSYPVYNVLGQRHTYGDLKADSDS
                     LAAVIDQLGLPEKSPVVVFGGQEYEMLATFVALTKSGHAYIPIDSHSALERVSAILEV
                     AEPSLIIAISAFPLEQVSTPMINLAQVQEAFAQGNNYEITHPVKGDDNYYIIFTSGTT
                     GKPKGVQISHDNLLSFTNWMITDKEFATPSRPQMLAQPPYSFDLSVMYWAPTLALGGT
                     LFTLPSVITQDFKQLFAAIFSLPIAIWTSTPSFADMAMLSEYFNSEKMPGITHFYFDG
                     EELTVKTAQKLRERFPNARIINAYGPTEATVALSAVAVTDEMLATLKRLPIGYTKADS
                     PTFIIDEEGNKLPNGEQGEIIVSGPAVSKGYMNNPEKTAEAFFEFENLPAYHTGDVGT
                     MTDEGLLLYGGRMDFQIKFNGYRIELEDVSQNLNKSRFIESAVAVPRYNKDHKVQNLL
                     AYVILKDGVREQFERDIDITKAIKEDLTDIMMSYMMPSKFLYRDSLPLTPNGKIDIKG
                     LINEVNKR"
     gene            13543..14787
                     /gene="dltB"
                     /locus_tag="VYN69_000466"
     CDS             13543..14787
                     /gene="dltB"
                     /locus_tag="VYN69_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEV1532795.1"
                     /translation="MMEFFQQLPHLEPYGNPQYFVYVIAATLPIFIGLFFKKRFAWYE
                     VLVSLFFIVTMLVGGKTNQLAALGIYLCWEILLLLFYKHYRKSKDGKWVFYLVSFLSL
                     LPIIFVKVQPAINGTQSLLGFLGISYLTFRSVGIIIELRDGVIKDFTLWEFLRFLLFM
                     PTFSSGPIDRFKRFNENYQAIPERDELMDMLDESVRYIMRGFLYKFILAHVLGETLLP
                     PLKNLALQSGGFFNLYALAVMYTFGLELFFDFAGYSMFALAISNLMGIRSPINFNKPF
                     LSRDLKEFWNRWHMSLSFWFRDFVFMRMVMVLTRKKVFKNRNVTSSMAYIVNMLIMGF
                     WHGVTWYYIAYGLFHGLGLVINDAWVRKKKTLNKERKKAGKAALPENRWIQFLGMVVT
                     FHVVMLSFLIFSGFLNNLWFKK"
     gene            14801..15040
                     /gene="dltC"
                     /locus_tag="VYN69_000467"
     CDS             14801..15040
                     /gene="dltC"
                     /locus_tag="VYN69_000467"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005591431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEV1532796.1"
                     /translation="MDIKSEVIEIIDELFMEDVSDMMDEDLFDAGVLDSMGTVELIVE
                     IENRFDIRVPVTEFGRDDWNTANKIIAGIVELQNA"
     gene            15033..16301
                     /gene="dltD"
                     /locus_tag="VYN69_000468"
     CDS             15033..16301
                     /gene="dltD"
                     /locus_tag="VYN69_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000919885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEV1532797.1"
                     /translation="MLKRLWMIFGPVLIAGLLVFLLIFFYPTEMHHNLGAEKRSAVAT
                     TIDSFKERSQKVRALSDPNVRFVPFFGSSEWLRFDGAHPAVLAEKYNRSYRPYLLGQG
                     GAASLNQYFGMQQMLPQLENKQVVYVISPQWFSKNGYDPAAFQQYFNGDQLTSFLKHQ
                     SGDQASQYAATRLLQQFPNVAMKDLVQKLASKEELSTADNEMIELLARFNERQASFFG
                     QFSVRGYVNYDKHVAKYLKILPDQFSYQAIEDVVKADAEKNTSNNEMGMENYFYNEQI
                     KKDLKKLKDSQKSFTYLKSPEYNDLQLVLTQFSKSKVNPIFIIPPVNKKWMDYAGLRE
                     DMYQQTVQKIRYQLESQGFTNIADFSKDGGEPFFMKDTIHLGWLGWLAFDKAVDPFLS
                     NPTPAPTYHLNERFFSKDWATYDGDVKEFQ"
     gene            16456..16896
                     /gene="adcR"
                     /locus_tag="VYN69_000469"
     CDS             16456..16896
                     /gene="adcR"
                     /locus_tag="VYN69_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001249319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent transcriptional regulator AdcR"
                     /protein_id="HEV1532798.1"
                     /translation="MRQLAKDINAFLNEVILQAENQHEILIGHCTSEVALTNTQEHIL
                     MLLSEESLTNSELARRLNVSQAAVTKAIKSLVKEGMLETSKDSKDARVIFYQLTDLAR
                     PIAEEHHHHHEHTLLTYEQVATQFTPNEQKVIQRFLTALVGEIK"
     gene            16896..17600
                     /locus_tag="VYN69_000470"
     CDS             16896..17600
                     /locus_tag="VYN69_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1532799.1"
                     /translation="MRYITVEDLSFYYDKEPVLEHINYCVDSGEFVTLTGENGAAKTT
                     LIKASLGILQPRIGKVAISKTNTQGKKLRIAYLPQQIASFNAGFPSTVYEFVKSGRYP
                     RKGWFRRLNAHDEEHIKASLDSVGMWEHRDKRLGSLSGGQKQRAVIARMFASDPDVFI
                     LDEPTTGMDAGSKNEFYELMHHSAHHHGKAVLMITHDPEEVKDYADRNIHLVRNQDSP
                     WRCFNVHENGQEVGHA"
     gene            17593..18399
                     /locus_tag="VYN69_000471"
     CDS             17593..18399
                     /locus_tag="VYN69_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1532800.1"
                     /translation="MLSLLSYDFIQRAFLAVIAMSLFSPVLGTFLILRRQSLMSDTLS
                     HVSLSGVAFGLVLGISPTVSTIAIVLIAAVFLEYLRTVYKSFMEIGTAILMSTGLAVS
                     LIVMSKGKSSSSMSLDQYLFGSIVTISEEQVISLFVIAAVVLILTFLFLRPMYILTFD
                     EDTAFVDGLPVRTMSILFNMVTGVAIALMIPAAGALLVSTIMVLPASIALRLGKNFKS
                     VMLLASAIGFLGMVAGLYISYYAETPASASITIIFVTVFILISLVRRFIK"
     gene            18409..19914
                     /gene="adcA"
                     /locus_tag="VYN69_000472"
     CDS             18409..19914
                     /gene="adcA"
                     /locus_tag="VYN69_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HEV1532801.1"
                     /translation="MKKISLLLASLCALFLVACSNQKQADGKLNIVTTFYPVYEFTKQ
                     VAGDTANVELLIGAGTEPHEYEPSAKAVAKIQDADTFVYENENMETWVPKLLDTLDKK
                     KVKTIKATGDMLLLPGGEEEEGDHDHGEEGHHHEFDPHVWLSPVRAIKLVEHIRDSLS
                     ADYPDKKETFEKNAAAYIEKLQALDKAYAEGLSQAKQKSFVTQHAAFNYLALDYGLKQ
                     VAISGLSPDAEPSAARLAELTEYVKKNKIAYIYFEENASQALANTLSKEAGVKTDVLN
                     PLESLTEEDTKAGENYISVMEKNLKALKQTTDQEGPAIEPEKAEDTKTVQNGYFEDAA
                     VKDRTLSDYAGNWQSVYPFLEDGTFDQVFDYKAKLTGKMTQAEYKAYYTKGYQTDVTK
                     INITDNTMEFVQGGQSKKYTYKYVGKKILTYKKGNRGVRFLFEATDADAGQFKYVQFS
                     DHNIAPVKAEHFHIFFGGTSQEALFEEMDNWPTYYPDNLSGQEIAQEMLAH"
     gene            complement(21085..21858)
                     /locus_tag="VYN69_000473"
     CDS             complement(21085..21858)
                     /locus_tag="VYN69_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1532802.1"
                     /translation="MIKDERVLELIEIIKKKKRIAVKELAEITFSSTSTLRRDLIFLE
                     NQGLIKRKHGYVTLSSMNTIELSHQIREGESTRQKRLIASLAKDFIRSGMCIYLDSST
                     TVYELCPYLSELDNLIIFTNGLHTAQTLSETVKDSSKIFITSGEVKHQSCSVVNYDKE
                     NSLLDHFNIDLAFCSARGIDDQYVYEASLSQAISKKNIIDKAHETILLIDSSKFYKTG
                     FFKINPLSKYTTFISDTLPDQKLLDAVELFDGEWVSDIQ"
     gene            22034..23437
                     /locus_tag="VYN69_000474"
     CDS             22034..23437
                     /locus_tag="VYN69_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEV1532803.1"
                     /translation="MEAVLAIDLGATSGRAIVGYLSENKLVMEEINRFSNLPIRVKGH
                     LSWDIDFLLAKILESIRLANTSYKILSIGIDTWGVDFGLVDNEGKLLLQPVHYRDERT
                     KGVLKEISEMTELEKLYSETGNQIMEINTLFQLFKARQESPDSFYKTNKILLMPDLFN
                     YLLTGKFATEKSIASTTQLFDPRSQNWNQNILKLFELDSSLLPEIVSEGNVLGRIKKE
                     YGLGDIPVVNVCSHDTASAIVSVPKTEGSLFISSGTWSLVGVELTSPLLTTESFSYGF
                     TNEVGKDGVITFLKNCTGLWIIEELRRSFERRGKAYSFDDIRTMVEKEKENLPLIDTE
                     STEFATESDMHKTLTEYLAYHHETREWTDGQLFKIVYESLAETYRKAIELLEELTHKV
                     YKRIYVIGGGARASYFNQMIADRTGKEVLTGLTEATAVGNIVVQLIAMGQLKGMEEAH
                     HVIEEFLQLESYYSQKN"
     gene            23552..24190
                     /locus_tag="VYN69_000475"
     CDS             23552..24190
                     /locus_tag="VYN69_000475"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001286141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HEV1532804.1"
                     /translation="MSDVKQELIKYGKKLVETDLTKGTGGNLSVFDREKQLMAITPSG
                     IDFFEIKESDIVVMDINGNVVEGERLPSSEWYMHLIQYQTRDDIDAIIHAHTTYATVL
                     ACLREPLPASHYMIAVAGKDVRVAEYATYGTKELAVNAAKAMEGRRAVLLANHGILAG
                     AQNLLNAFNIVEEVEYCAKIYCLAKNFGEPVVLPDEEMDLMAEKFKTYGQRK"
     gene            24202..24642
                     /locus_tag="VYN69_000476"
     CDS             24202..24642
                     /locus_tag="VYN69_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000912112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HEV1532805.1"
                     /translation="MLKHIPKNISPDLLKTLMEMGHGDEIVLADANYPSASCANKLIR
                     CDGVNIPELLDSILYLMPLDSYVDSSIQFMNVVSGDDIPKIWGTYRQMIEGHGTDLKT
                     ITYLRREDFYERSKKAYAIVATGETSLYANIILKKGVVVERENV"
     gene            24635..25066
                     /locus_tag="VYN69_000477"
     CDS             24635..25066
                     /locus_tag="VYN69_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1532806.1"
                     /translation="MFNRGILVASHGNFASGALMTAEMFVGETTNDRVRTLGLMPGEN
                     IVEFEHYFKNQVNELLDSNQEVIVLTDLIGGSPNNVALSRFLNLDSVDIVTGFNIPLL
                     VELISSYDSKINLEEIVQNAQNSLFNVKQQLNVEEEEDLCL"
     gene            25057..25527
                     /locus_tag="VYN69_000478"
     CDS             25057..25527
                     /locus_tag="VYN69_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000020992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1532807.1"
                     /translation="MSIEFVRIDDRLVHGQVVTTWLKKYDIEQVIIVNDRISEDKTRQ
                     SILKISAPVGLKIVFFSVKRFVEVLNSVPIKKRTMLIYTNPKDVYDSIEGNLKLEYLN
                     VGQMSKTEENEKVTGGVALGEEDKYYFKKIVDKGTRVEIQMVPNDKVTMLEKFL"
     gene            25552..26325
                     /locus_tag="VYN69_000479"
     CDS             25552..26325
                     /locus_tag="VYN69_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1532808.1"
                     /translation="MLFTQALLVTLVGIIATIDYNGPLFMIHRPLVTSAMVGLVLGDF
                     TQGVLIGSALELTWLGVTGIGGYTPPDTISGAIIGTAFGILSGQGETAGIAIAVPIAV
                     ATQQLDVLAKTLDVYFVKKADNDAKNGDYSKIGFYHYSSLVLITLFKIVPIFLAIMLG
                     GEYVADLFAKVPPIVMQGLNSAGALLPSIGFGMLLNMMLKKNMWVFLLIGFICSVYGG
                     MSTIGISLVGIAVAYFYDMIGSKPQETTSSSDVEEDLDL"
     gene            26322..27122
                     /locus_tag="VYN69_000480"
     CDS             26322..27122
                     /locus_tag="VYN69_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1532809.1"
                     /translation="MMNNKVTKVELKKVFKRSFMYGSSWNYERMQNLGFLYTILPVLK
                     KLYPDKDSASPAMKRHLEFFNTHQTAAPFILGVTSAMEEQEGNEGAASITGIKVGLMG
                     PLAGLGDSLFWLTLVPICFSIGASYSKDGGALGIFIALILFNIINIPVKYFGLKYGYT
                     KGSSLIQENNTKGTLNRVTSMATALGLVLVGGLIPSMVGINFGLEFKQGELVISVQEM
                     ITKLIPGFIPMALTLLMCKLIRKGKNPVVLIFSVMAIGVILVVLGILK"
     gene            27181..29475
                     /locus_tag="VYN69_000481"
     CDS             27181..29475
                     /locus_tag="VYN69_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1532810.1"
                     /translation="MKLWYKKAASNWNEALPIGNGHLGGMIYGSATKECIQLNDETIW
                     YRGKSDRNNPDSLLHLKKIREYLLDGEIQKAEELIKLTMFATPRDQSHYELLGELYIE
                     HIDIQSCALSLYERELDLDTAISNVVFEPNSCNLQIKREYFTSFNKNILCCRIVSSVQ
                     NTLNLNINLGRNKRFNDEVSKLDSSTILMSASAGGRKGVQFKVVCHSKVTDGEVSVLG
                     ETIVIRNATEVFLYLKSMTDYWGNIDISSLQGEFSSIDYFTEKDEHVKKYQEQFNRVD
                     FKLDYSKDCLSIPTNLLLENTKKYSNYLTNLLFHYGRYLLISSSQPNGLPANLQGIWC
                     DELNPIWGSKYTININTQMNYWMVGPCDLPEVEYPLFDMLERMREPGRLTAKKMYGAR
                     GFTAHHNTDGFGDTAPQSHAMGAAIWVLTIPWLCTHIWEHYLYFQDERILTEHFEMIK
                     EAFLFFEDYLFEVDGYLMTGPSVSPENKYRLKNGIEGNACLSSTIDNQILRYFCDSCI
                     GIAKQLGDNSDFISRVKELKKKLPKTKIGSNGQIQEWLEDYEEVEPGHRHISPLFGLY
                     PYNEIDIHKTPELAEAAKITINRRLSNANFLSSQEREQAINNWLVSGLHASTQTGWSA
                     AWLIHFFARLYQGEPAYNQINGLLNNATLGNLFLDHPPFQIDGNLGLVSGICELLVQS
                     HHNWLSLIPALPSAWSEGEVKGFRVRGGYKVSFAWKNGDITFLKLEGGNKDQKVRVRI
                     YGKNTDVQNIELVFNSEKIIELNF"
     gene            29485..32601
                     /locus_tag="VYN69_000482"
     CDS             29485..32601
                     /locus_tag="VYN69_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001031112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1532811.1"
                     /translation="MNKEKIKRKLITILFVCIGMLCFGLLAGVKADNRVQMRTTINNE
                     SPLLLSPLYGNDNGNGLWWGNTLKGAWEAIPEDVKPYAAIELHPAKVCKPTSCIPRDT
                     KELREWYVKMLEEAQSLNIPVFLVIMSAGERNTVPPEWLDEQFQKYSVLKGVLNIENY
                     WIYNNQLAPHSAKYLEVCAKYGAHFIWHDHEKWFWETIMNDPTFFEASQKYHKNLVLA
                     TKNTPIRDDAGTDSIVSGFWLSGLCDNWGSSTDTWKWWEKHYTNTFETGRARDMRSYA
                     SEPESMIAMEMMNVYTGGGTVYNFECAAYTFMTNDVPTPAFTKGIIPFFRHAIQNPAP
                     SKEEVVNRTKAVFWNGEGRISSLNGFYQGLYSNDETMPLYNNGRYHILPVIHEKIDKE
                     KISSIFPNAKILTKNSEELSSKVNYLNSLYPKLYEGDGYAQRVGNSWYIYNSNANINK
                     NQQVMLPMYTNNTKSLSLDLTPHTYAVVKENPNNLHILLNNYRTDKTAMWALSGNFDA
                     SKSWKKEELELANWISKNYSINPVDNDFRTTTLTLKGHTGHKPQINISGDKNHYTYTE
                     NWDENTHVYTITVNHNGMVEMSINTEGTGPVSFPTPDKFNDGNLNIAYAKPTTQSSVD
                     YNGDPNRAVDGNRNGNFNSGSVTHTRADNPSWWEVDLKKMDKVGLVKIYNRTDAETQR
                     LSNFDVILYDNNRNEVAKKHVNNLSGESVSLDFKEKGARYIKVKLLTSGVPLSLAEVE
                     VFRESDGKQSEEDIDKITEDKVVSTNKVATQSSTNYEGVAALAVDGNKDGDYGHHSVT
                     HTKADSNAWWQVDLGEEFTVSKVDIYNRTDAEPQRLSNFDVIFLSSSGEEVFRRHFDK
                     VVDGLLSLKVPSVGAKLVKIELKSAAIPLSLAEVEVYGSKRTPKKLSNIALTKETRQS
                     STDYNGFSRLAVDGNKNGDYGHHSVTHTKEDSPSWWEIDLAQTEELEKLIIYNRTDAE
                     IQRLSNFDIIIYDSNDYEVFTQHIDSLESNNLSIDLKGLKGKKVRISLRNAGIPLSLA
                     EVEVYTYK"
     gene            32684..34450
                     /locus_tag="VYN69_000483"
     CDS             32684..34450
                     /locus_tag="VYN69_000483"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HEV1532812.1"
                     /translation="MIQHPRIGIRPTIDGRRQGVRESLEVQTMNMAKSVADLISSTLK
                     YPDGEPVECVISPSTIGRVPEAAASHELFKKSNVCATITVTPCWCYGSETMDMSPDIP
                     HAILGFNGTERPGAVYLAAVLASHAQKGIPAFGIYGRDVQEASDTDIPEDVKEKLLRY
                     ARAALATGLMRDTAYLSMGSVSMGIGGSIVNPDFFQEYLGMRNESVDMTEFTRRMDRS
                     IYDPEEFERALKWVKENVKEGFDHNREDLVLSREEKDRQWEFVIKMFMIGRDLMVGNP
                     RLAELGFEEEAVGHHALVAGFQGQRQWTDHFPNGDFMETFLNTQFDWNGIRKPFVFAT
                     ENDSLNGVSMLFNYLLTNTPQIFADVRTYWSPEAVKRVTGHTLEGRAAAGFLHLINSG
                     SCTLDGTGQATRDGKPVMKPFWELEESEVQAMLENTDFPPANREYFRGGGFSTRFLTK
                     GDMPVTMVRLNLLKGVGPVLQIAEGYTLELPEDVHHTLDNRTDPGWPTTWFAPRLTGK
                     GAFKSVYDVMNNWGANHGAITYGHIGADLITLASMLRIPVNMHNVPEEDIFRPKNWSL
                     FGTEDLESADYRACQLLGPLHK"
     gene            34596..35747
                     /locus_tag="VYN69_000484"
     CDS             34596..35747
                     /locus_tag="VYN69_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HEV1532813.1"
                     /translation="MATFYVPAVNLIGKGVVNEVGPYIKELGYKKALLVTDKYIEGSD
                     ILPKVLKPLDTEGIEYVIFSDVEPNPTCKNVTDGVAALQEHGCDFIISLGGGSPQDAA
                     SCISIMATNGGKPQDYEGLHKSAKKGLPVVAINTTAGTSAEITINYVITDEERKVKMV
                     MVDKNSLALISVNDPELMLSKPKGLTAATGMDALTHAVEALVTPGAYDVTKKLSIGAI
                     ELIKEYLPRAVENGHDIEAREGMVNAIFLGGMSFNNAGLGYVHSMAHQLGAVYNLPHG
                     VCCAMLLPVIERENAKRVPEAFRNVAKALGLHVEGKSDQECADYAIAEIEKLSETVGI
                     PKKLTELGIEEKDFDFEYLSKNALIDACAPGNPFMPTLEETIAFYKELF"
     gene            35892..36776
                     /locus_tag="VYN69_000485"
     CDS             35892..36776
                     /locus_tag="VYN69_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1532814.1"
                     /translation="MIFLIVCVLLLVIVTLSTVYVVRQQSVAIIERFGKYQKVANSGI
                     HIRLPFGIDSIAARIQLRLLQSDIVVETKTKDNVFVMMNVATQYRVNEQSVTDAYYKL
                     IRPESQIKSYIEDALRSSVPKLTLDELFEKKDEIALEVQHQVAEEMTTYGYIIVKTLI
                     TKVEPDAEVKQSMNEINAAQRKRVAAQELAEADKIKIVTAAEAEAEKDRLHGVGIAQQ
                     RKAIVDGLAESITELKEANVGMTEEQIMSILLTNQYLDTLNTFASKGNQTIFLPNTPN
                     GVDDIRTQILSALRAEKK"
     gene            37130..37381
                     /locus_tag="VYN69_000486"
     CDS             37130..37381
                     /locus_tag="VYN69_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657761.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1532815.1"
                     /translation="MKEFQFERKQRFSLRKYAIGACSVLLGTSLFFAGMGAQPVQATE
                     TSSALISSHYLDEQDLSEKLKSELQWFELENKLLNLWEH"
     gene            37369..37659
                     /locus_tag="VYN69_000487"
     CDS             37369..37659
                     /locus_tag="VYN69_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532816.1"
                     /translation="MGALGYYEGFVPYVSNQYKNQTEEEGKPLSDKYIFEKILGKTYA
                     AFKKDQINERVEKLGKLKPITINYNGKSEVIDSKEKLQKLMNKAVKDEVAQI"
BASE COUNT        11739 a         6550 c         8232 g        11339 t
ORIGIN      
        1 tccaatacaa tggttcatgg tactacctaa acagcaatgg tgctatgaaa gcaagccaat
       61 ggttcaaagt atcagataaa tggtactatg tcaatggctc aggtgccctt gcagtcaaca
      121 caactgtaga tagctataga gtcaatgcca atggtgaatg ggtaaactaa acttaatata
      181 actagttaat actgacttcc tgtaagaact ctttaaagta ttccctacaa ataccatatc
      241 ctttcagtag ataatatacc cttgtaggaa gtttagatta aaaaataact ctgtaatctc
      301 tagccggatt tatagcgcta gagactacgg agtttttttg atgaggaaag aatggcggca
      361 ttcaagagac tctttaagag agttacgggt tttaaactat taagccttct ccaattgcaa
      421 gagggcttca atctctgcta gggtgctagc ttgcgaaatg gctccacgga gtttggcagc
      481 gccagatgtt ccacggaggt agtgaggagc gaggccgcgg aattcacgaa ctgcgacgtt
      541 ttctcctttg aggttaatca atcgtttcaa gtgttcgtag gcgatcttca tcttgtcttc
      601 aaaggtcaaa tcaggtagga tttctcctgt ttcaaagtaa tggttgattt ggttgaagag
      661 gtaaggattt cccatggcag ctcggccaat catgactgcg tcagcaccaa cttcttcgat
      721 gcgttgcttg gcttcttgga cagtacggat atcaccgttg gcgatgaatg gaatcttggt
      781 tagagcttgg gcaaccttgt aaagggtctc aaggtctgcg tggccagtat acatttgttc
      841 acgggtacgg ccatgcatgg cgagggcaga aacacctgca gcttcagcag cgagggcatt
      901 ttctactgcc agagatgggt ccgcccagcc ggtacgcatt ttgacagtaa gtgggatatc
      961 aaggacagac tggaccttgt tgatgataga gtagatcttg tcaggatcct tgagccacat
     1021 agcaccagct tcgttcttca cgattttgtt gacagggcag cccatgttga tatcgacgat
     1081 atcggtcttg gtgttttctt ggatgaattc tgctgcgcgt gctaggctgt cttcatcgct
     1141 accaaaaagt tggatagaga cagggttttc gccctcatcg atatgaagca tgtgcagggt
     1201 tttttcgttg ttgtattgga ttcccttgtc agagaccatt tccattacaa cgagtccagc
     1261 tccgagctct tttgcgatgg tacgaaaggc tgagttggtc acgccagcca taggcgctaa
     1321 aacggtacga ttgggaatct caacattgcc aatcataaaa ggtgtattaa gatttgtcac
     1381 gaatgagttc ctccaggtcc ttttcatcaa agttgtaagt agtttggcag aattgacaag
     1441 tgatttctgc cccgtggtct tcctctttca tttcctgtaa gtctgagctt ggaaggctgg
     1501 caagagcgtt cataaagcgt tcatggctac agtcacattg gaaacggatt tcttcttcag
     1561 aaagacgctt gtaggcttcg tccccgtaga tagccttgag gagggcttcg atatggtcgt
     1621 cgctttcgag aagagtagag atagctggca tttcttggat gcgtttttca aagcgagcaa
     1681 tctcttcttt cttggctcct ggcaagactt gaactaggaa accacctgca accttgacct
     1741 tgtcttcctc gtccaaaagg acattgaggc cgaccgctga aggcgtttgt tggctttcag
     1801 taaggtaaaa ggcaaggtct tcaccgattt ctccagagat gaggggagtt atagagttgt
     1861 aaggatttcc agcaccgtag tctgtgataa cgaggaattg accatttcca acaaaaggtc
     1921 cgactaggac ttcaccagtc gcagtctttt tgatgtcaac accaggattt tgaacatagc
     1981 ctttgacgtt ccccttggta tcagcgacgg tgataatagc acctagagag ctagagccca
     2041 acaccttaac tgtaagtttg gtatttcctt tttcattggc tgcgagaatc tggctggcga
     2101 taagagttcg accaagcgct acagttgagc tagcttgggt ttgatgtttt tcttgagcag
     2161 tgcggacggt ttccgtgcta tcaaggacaa aagcacgaaa ggctccgctt tctgatatag
     2221 ttttaataat tttatccata gctactattt tagcataaaa atgcccaaag ggggagccgt
     2281 gtgtttactg attttcagga taatggacca ggaaatcagc atgaaaataa aaagagaaac
     2341 agattatttt agcatttgta agatttatgc tatgcttaag gtagaaaatg aaaggggtaa
     2401 caaatgtatt taggagattt gatggagaaa gccgagtgtg gtcaattttc aatcctttcc
     2461 tttctattac aagagtctca gacgaccgtc aaggctgtaa tggaagaaac aggattttca
     2521 aaagcaaccc taaccaaata tgtcaccctg ctcaatgaca aggctttgga tagtggctta
     2581 gagctggcta ttcactcaga agatgaaaat ctgcgtctgt ctatcggtgc agctaccaag
     2641 gggagagata ttcggagctt gtttttggag agtgctgtta aataccagat tttggtttat
     2701 cttctctact accaacagtt tttagcccat cagctggctc aagaattggt gattagcgag
     2761 gctacgcttg gtcgtcactt ggctggttta aatcagattt tgtcagaatt tgatttatcc
     2821 atccaaaatg gacgttggcg aggtccagag catcagattc gctatttcta tttctgtctt
     2881 ttccgaaagg tctggtcgag tcaggaatgg gaaggtcaca tgcagaaacc agagagaaaa
     2941 caggagattg ccaatttaga ggaaatctgc ggtgcaagtt tgtctgtggg gcagaaattg
     3001 ggcttggttc tctgggctca catcagtcaa caacgtcttc gggtcaatgc ttgtcagttt
     3061 caagtcatag aagagaaaat gcgagggtat tttgacaata ttttctacct tcgtttgctg
     3121 agaaaggttc cgtccttttt tgctgggcaa catattccac taggagttga ggatggtgag
     3181 atgatgatat tcttctcttt tctcctatct catcgcattc ttcctcttca tactatggag
     3241 tatattcttg gttttggagg gcagttggca gatttactga cgcaattgat tcaagaaatg
     3301 aagaaggagg aactattggg ggattataca gaggaccatg tcacctatga actcagtcag
     3361 ctttgtgctc aagtctatct ctataagggc tatattttac aggatcgcta caagtaccag
     3421 ttagagaatc gtcatccata tttactgatg gaacatgatt ttaaagagac agcagaggag
     3481 atttttcatg ctctacctac ttttcaacag gggacagatt tagataagaa gattctctgg
     3541 gaatggctcc agttaatcga atatatggct gaaaacggtg gccagcatat gcggattggt
     3601 ctggatttga catctggttt tcttgtcttt tcaaggatgg cagccatttt gaaacggtat
     3661 ttggaataca atcgttttat taccattgaa gcttatgacc ctagtcggca ttatgatttg
     3721 ctggttacca ataacccgat tcataagaag gaacagcccc cagtttatta tttaaaaaat
     3781 gacttggata tggaagattt ggcagggatt cgtcagttat tattcactta aaaggcttgg
     3841 ttaatccagg tcttttttgt gaaattcaca caatctcctc acattttttt aaaaattaaa
     3901 aaaagttgat aaacaagaaa gcgctttatt ttgtatacta gtaagtgtaa agaggaaaca
     3961 cctcaagatc tttatcagga ggacagtaca tgtcacaaga aaaatacatc atggccattg
     4021 accagggaac tacaagttct cgtgccatca ttttcaacaa aaaaggggaa aaggttagct
     4081 cgagtcaaaa agagtttacc cagattttcc ctcaggcagg ttgggttgag cacaatgcca
     4141 atgaaatttg gaactctgtt cagtcagtta ttgcgggtgc tttcatcgaa agtggtgtca
     4201 agccaaatca aatcgaggca atcgggatta ccaaccaacg tgaaacaacg gttgtctggg
     4261 ataagaaaac aggacttcct atctacaatg ctatcgtttg gcagtcacgc cagacagcac
     4321 ctttggctga gcaactaaaa agccaaggtt atgtggaaaa attccatgaa aagactggtt
     4381 tgattattga tgcttacttc tctgctacca aggttcgttg gattttggat catgtagaag
     4441 gtgctcaaga gcgagcagaa aaaggggaat tgctctttgg tactatcgat acttggttgg
     4501 tttggaaatt gactgacggt gcggctcacg tgactgacta ctcaaatgca gctcgtacca
     4561 tgctttataa cattaaagaa ctcaaatggg atgatgagat tttggaaatc cttaacattc
     4621 cgaaggctat acttccagaa gttcgttcta actccgaaat ctacggcaag acagctccat
     4681 tccatttcta cggtggagag gtgccaatct caggtatggc tggggaccaa caagcagccc
     4741 tctttggaca gttggctttt gagccaggta tggttaagaa tacttatgga acaggctctt
     4801 tcatcatcat gaatactggg gaagagatgc agttgtctga aaacaacctc ttgacaacca
     4861 ttggttacgg aatcaacggt aaggtttatt atgccttgga aggttctatc ttcatcgcag
     4921 gaagtgctat tcagtggctt cgtgacggtc ttcgcatggt tgaaaattca ccagaatctg
     4981 aaaaatacgc tcgtgattct cacaacaacg atgaagttta tgtcgttcca gcctttacag
     5041 gtctaggcgc tccatactgg aaccaaaatg ctcgtggttc cgtctttggt ttgactcgtg
     5101 gaacaagcaa agaagacttt atcaaggcga ctttgcaatc tattgcttat caagtgcgtg
     5161 atatcatcga caccatgcaa gtggatactc agaccgccat tcaagtactg aaggtggatg
     5221 gtggtgcagc catgaacaac ttcctcatgc agttccaggc ggatatttta ggcattgaca
     5281 ttgcacgtgc taaaaacttg gaaacaacag ctctaggagc ggccttccta gcaggtttgt
     5341 cagtagggta ctggaaagac ttggacgagt tgaaactctt gaacgagaca ggagaactct
     5401 ttgagccatc tatgaacgaa tctcgcaagg aacaactcta caagggctgg aagaaggctg
     5461 tgaaagcaac tcaagtcttt gcggaagtag acgactaata ctggcagaat aaagcgattt
     5521 atttagaaag tgtgtaaata tggaattttc aaaaaaaaca cgtgaattgt caattaaaaa
     5581 aatgcaggaa cgcaccctgg acctcttgat tatcggtgga ggaatcacag gagctggtgt
     5641 agccttgcag gcggcagcta gcggtcttga gactggtttg attgaaatgc aagactttgc
     5701 agaaggaaca tctagtcgtt caacaaaatt ggttcacgga ggtcttcgct acctcaaaca
     5761 attcgacgta gaagtggtct cagatacggt ttctgaacgt gcagtggttc aacaaatcgc
     5821 tccacacatt ccaaaaccag atccaatgct cttaccagtt tacgatgaag atggagcaac
     5881 ctttagcctc ttccgtctta aagtagccat ggacttgtac gacctcttgg caggtgttag
     5941 caacacacca actgcgaaca aggttttgag caagaaagaa gtcttggaac gccagccaaa
     6001 cttgaagaag gaaggcttgg taggaggtgg agtgtatctt gacttccgta acaacgatgc
     6061 gcgtctcgtg attgaaaaca tcaaacgtgc caaccaagac ggtgccctca ttgccaacca
     6121 cgtgaaggca gaaggcttcc tctttgacga aagtggcaag attacagttg ttgtagctcg
     6181 tgatctcttg acagatcaag tatttgaaat caaggcccgt ctggttatta atacaacagg
     6241 tccttggagt gataaagtac gtaatttgtc taataaggga acgcaattct cacaaatgcg
     6301 cccaactaag ggagttcact tggtagtgga ttcaagtaaa atcaaggttt cacagccagt
     6361 ttacttcgac acaggtttgg gtgacggtcg tatggtcttt gttctcccac gtgaaaacaa
     6421 gacttacttt ggtacaactg atacagacta cacaggtgat ttggagcatc caaaagtaac
     6481 tcaagaagat gtagattatc tacttggcat tgtcaacaac cgcttcccag aatccaacat
     6541 caccattgat gatatcgaaa gcagctgggc aggtcttcgt ccattgattg cagggaacag
     6601 tgcctctgac tataatggtg gaaataacgg taccatcagt gatgaaagct ttgacaactt
     6661 gattgcgact gttgaatctt atctctccaa agaaaaaaca cgtgaagatg ttgagtctgc
     6721 tgtcagcaag cttgaaagta gcacctctga gaaatatttg gatccatctg cagtttctcg
     6781 tgggtctagc ttggaccgtg atgacaatgg tcttttgact cttgctggcg gtaaaatcac
     6841 agactaccgt aagatggctg aaggagctat ggagcgcgtg gttgacatcc tcaaagcaga
     6901 atttgaccgt agctttaaat tgatcaattc taaaacttac cctgtttcag gtggagaatt
     6961 gaacccagca aatgtggatt cagaaatcga agcctttgcg caacttggag tatcacgtgg
     7021 tttggatagc aaggaagctc actatctggc aaatctttac ggttcaaatg caccgaaagt
     7081 ctttgcactt gctcacagct tggaacaagc gccaggactc agcttggcag atactttgtc
     7141 ccttcactat gcaatgcgca acgagttggc tcttagccca gttgacttcc ttcttcgtcg
     7201 taccaaccat atgctcttta tgcgtgatag cttggatagc atcgttgagc cagttttgga
     7261 tgaaatggga cgattctatg actggacaga agaagaaaaa gcaacttacc gtgctgatgt
     7321 cgaagcagct ctcgctaaca acgatttagc agaattaaaa aattaagaaa aaataaaaga
     7381 ggtggagggc agcattcctt gtcgctcgtc ccttcttttt aatggagaca gaaagatgat
     7441 gaatgaatta tttggagaat ttctagggac tttaatcctg attcttctag gaaatggtgt
     7501 tgttgcaggt gtggttcttc ctaaaaccaa gagcaatagc tcaggttgga ttgtgattac
     7561 tatgggttgg gggattgcag ttgcggttgc agtatttgta tctggcaagc tcagtccagc
     7621 tcatttaaat ccagctgtga caattggtgt agctttaaaa ggtgacttgc cttgggcttc
     7681 cgttttgcct tatatcttag cccagttcgc aggggctatg ctgggtcaga ttttggtttg
     7741 gttgcaattc aaacctcact atgaggcaga agaaaatgca ggcaatatcc tggcaacctt
     7801 cagtactgga ccagccatca aggatactgt atcaaacttg attagcgaaa tccttggaac
     7861 ctttgttttg gtgttgacaa tctttgcttt gggtctttac gattttcagg caggtatcgg
     7921 aacctttgca gtgggtacct tgattgtcgg tatcggtcta tcgctaggtg ggacaacagg
     7981 ttatgccttg aatcctgcgc gtgaccttgg acctcgtatc atgcacagta tccttccaat
     8041 ttcaaacaag ggagacggag actggtctta tgcttggatt cctgttgtgg gacctgttat
     8101 cggtgcagcc ttggcagtgc ttgtattctc acttttctag tttatactct tcgaaaatca
     8161 aattcaaacc acgtcatcgt cgccttaccg tactcaagta cagcttgcgg ctagcttcct
     8221 agtttgctct ttgattttca ttgagtatta gaaaacaatt atgttgatag agcttgggca
     8281 agagtccaat ttcagcaaaa aatgaagtaa atcttctcat aataaaacgc atcatatcaa
     8341 gcacgaaaat tccacgaggt caactacagt cagaaagctg aacaacaagc caaaacgccc
     8401 aaaaaaggcg gcaaaaagca agcacctgca agcaacgtgc cgaaatagtc aaatcctgat
     8461 tatgtcaacg aattagaccc aaaaatcgtt gatatgctag tagaatttca caagtcacaa
     8521 ggcactttgg aaactcccga ggcgcaagca gaaatcgccc aaaaacgtga agaaatcgag
     8581 caaaggagag ctgagcttga ggataaaaaa caagagcttt tgaaccgctt gaacaaatag
     8641 agtttcgcaa gtattatgct tacaaattac ttgagcaatt aactaaaata taaaccctgc
     8701 ctttatatct aggcagggtt tatattttag aaattcacgt aggttgttac ggtttttaca
     8761 tacccagtat agtttgagtt tctatagtat tcagtgataa acttccattt tctttgagca
     8821 acatggatat aagtacttgt tatgtagtat ggatatgggc tttgtgaatc caagtaagac
     8881 tgataagctt gtataccaaa atatgctcca ccaatcattg caccccatgg cccccccaat
     8941 aaagcaccta tcctaccaat catataactg attccagcac cagtcatgaa gttagcgaat
     9001 gtgttagctt gtttattccc atgtattgtg ttgacgtaat tccaaacatt aggatcgtat
     9061 gatctaaaag atatatttag gtcgatttca ttcttttgat aagccatata aaatgcccca
     9121 ttgatataga cgccgtcagc acgtcgttca atagtgtcta cacttccatc tggattgaca
     9181 acctcaagaa cttcatcgct taaaatattt acttgcgtat ctccgaaccg cactgatgag
     9241 ccattctcaa actgagcctc accagataca actttagagt ttgccgataa gctatcatca
     9301 gcaaaaacaa caagcgacgg ggaaatgcta gacatacaga aaacagacat aactagcaaa
     9361 cacatgcatt taaacatctt agacataacg gaaactcttt tgtatttttg atttttttca
     9421 acttttatta tacaataaaa ccaaataaaa agaaagcggt aacaatatgc ttaatgcgaa
     9481 aattttttat atatttttat gtttgatcgt tatcgaaact acaggcttgt tgttgttgaa
     9541 aagaggtctc gaaatgggtt atttagacac agaagctatt atcctcgcag ttttttcatt
     9601 tgctttttac aacctatgtt cattcgcttg ggtctgctct acaataaaaa acaataaaaa
     9661 ataaatagac gtattttcaa aaaaaaaaaa aaaatgcata tttatcttag caaaatgacg
     9721 atttaaatcg tcgttttttt gtagtacgac gggcatgtcg tatatctgag gtgtaagtcc
     9781 tcggtgggca cctgctaccg gtgaacccaa tagcgattcc caagcctgac tatcgtgagg
     9841 tagcagggag aggaagggat agcgaaatcg tggctctacg aacaggaacg tgatagtaag
     9901 gcgtatatag cggataagga ggcttcaaac tctaaagtcc aaaaaggtag tcgtaaccta
     9961 tatgtgtaaa tcacgagagt aattgaattc ggactaaggt ttgtgtgaaa aagataaatc
    10021 tttctagagt ctaaagactc tgcgtcagat ttcctatttt cactgtaacc ttttaacgtc
    10081 ctcatatctt gtataaacga ggaaagatgt acgacttatc ccgtgaggtt tcatgagcgc
    10141 tgaaagcgta gtaacaacga atcatgagaa gtaagccgag cccatagtag tgaggaaact
    10201 tccgtaatgg aagtggagcg aaggggtgaa tactcaaaca gtctggggag agactgtttg
    10261 aggtctgtcg ctagaaagag aaaacgacag atcgaagtaa tcctacttca cttgtgtctg
    10321 taaaatgagt ggtctgatag aactggactt tgacacgtaa tgactagatg aaggttcacc
    10381 aatataagat gtccctcagg caccaaaaca agaaaggaat acgcacatgt caaaactgct
    10441 agataagata ttatcacgcg aaaatatgct ggaagcctac aatcaagtaa aatccaataa
    10501 aggctcagct gggattgatg gaatgactat cgaagagatg gataattatc tcagacaaaa
    10561 ctggcgcttg actaaggaac tgataaaaca gagaaaatat aagcctcaac cagttcttag
    10621 agttgagata cctaaaccag acggaggcat ccgtcaacta ggaattccaa cagttatgga
    10681 tagaatgatt caacaggcca ttgtccaagt catgagcccc atttgtgaac cccatttctc
    10741 agatacgagt tatggtttca gaccaaatag gtcatgtgaa aaagccatca tgaagctctt
    10801 agaatactta aatgacggct atgagtggat agtggatata gacctagaga aatttttcga
    10861 tacagttcct caagatagat tgatgtcctt agtacataac gctgaagtgg aggtgttaga
    10921 gtcagaaact aatcttgaaa ttttgctcaa aggttggaag ttagttgcta gaaatcctag
    10981 attaagactt tttgtatcag caaatttatt ggaaattttt tcaaatacga tttgggtttc
    11041 ttccattata cttgtttttg taacggagtt attaaataaa acggaaagtt actggggata
    11101 ttctaataca gcatactcta ttggtattat aattagtggc ttaattgctt ttaggctatc
    11161 tgaaaagttc cttgctgcta aatgggaaag tattcttttt cctcttgtag ctatggcaat
    11221 agtgacactg actattctat attttccaaa cgcacagatg tttttattat tttcagcttt
    11281 agttggtatg ttatcgcaac taaaagaagt tcctgaaagt gtatttcttc aggaaacagt
    11341 agaggaaaat catttagtta atgtctattc cgttcttgaa gtgatttcta cattagcatt
    11401 ttcagtattt gttttgctaa tgagctatat tactgagaat tttggtatta gcatcagttt
    11461 ttggctatca gccatttgtc tgatgataga agctatttta atttatatca gacgagatta
    11521 ttttaaatga gagagtggtt aaaaagtctt taaaaatcag aaaagcgcat agtatcaggt
    11581 gttgaaaaaa cttgatattg tgtgttttat tatgggaaga tttacttcat tgtctcctga
    11641 aatggagttt tgaagcagga tcactctatt tgaaaatata catagaaaaa ctgtataatc
    11701 cttaaaataa gctgaaatct attggaaaca tacggtaaac aattgaaaag gtactatata
    11761 aagtggtaca atggtagaaa aataagctag taagaatcac tcttatttta gcgaaaaatt
    11821 acagaaatga atggtttttc ttgatgaaac agagaaaaga attgtacctc tttcttggtc
    11881 ggacagcctt gtattttctt atctttctag ggctgcttta cttctttagc tatcttggtc
    11941 agggtcaagg aagctttatc tataatgaat tttaacttga aggagaatcc ctattgtgtc
    12001 aaataaacca atagcagata tgattgaaac cattgagcat tttgctcaga cacagcctag
    12061 ctatcctgtc tataatgttt tggggcagag acacacttat ggcgatttaa aggctgattc
    12121 ggatagtttg gctgcagtca ttgaccaact aggcttgcct gagaagtctc ctgtggttgt
    12181 ttttggtggc caagaatatg aaatgttggc aacctttgta gcgctgacta agtcaggtca
    12241 tgcctacatt ccaattgata gccattcggc cttggagcga gtttcagcta ttttagaagt
    12301 agcagagcca agcttgatta ttgccatttc agcctttccc ttggagcagg tttctacacc
    12361 aatgataaat ctagctcagg ttcaagaagc ctttgcccaa gggaacaact atgaaatcac
    12421 gcatccagtc aagggagatg ataattacta cattatcttt acttctggta cgactggtaa
    12481 gcctaaggga gtgcagattt cacatgataa tctcctcagc tttacaaact ggatgattac
    12541 ggataaggaa tttgcgacac cgagtcgtcc gcaaatgctg gcacagccac cttattcttt
    12601 tgacttgtct gtcatgtatt gggcaccgac cttggcactt ggtggtacgc ttttcactct
    12661 tccttcagtc atcactcagg actttaagca actctttgcg gctatctttt cattgccaat
    12721 cgctatctgg acatcaacac catcctttgc agatatggcc atgttgtctg aatacttcaa
    12781 cagtgaaaaa atgcctggaa tcacgcattt ctactttgat ggtgaagaat tgacggtcaa
    12841 aacagctcaa aaactgcgcg agcgtttccc aaatgcccgt atcatcaatg cttacggccc
    12901 aacagaagcg acagtagctc tgtcagcagt tgccgtgaca gacgagatgt tagcgactct
    12961 caaacgccta ccaatcggct ataccaaggc tgattctcca acctttatca ttgacgagga
    13021 aggaaataaa ctgccaaatg gtgagcaggg agaaatcatt gtttctgggc cagctgtttc
    13081 aaaaggttat atgaacaatc ctgaaaaaac agcagaagcc ttctttgagt ttgaaaatct
    13141 gccagcctat cacacaggcg atgtgggaac catgacagat gagggcttgc ttctctacgg
    13201 cggacgcatg gacttccaga ttaagtttaa cggttaccgc attgagttag aagatgtctc
    13261 tcaaaaccta aacaagtctc gctttatcga atctgctgtc gcagtaccgc gctataacaa
    13321 ggaccacaag gtacaaaatc tattggctta tgtcatctta aaagacggtg ttcgtgagca
    13381 gtttgagcga gatatcgata ttaccaaggc catcaaggaa gacctgacag acatcatgat
    13441 gtcctatatg atgccatcta aattccttta ccgagacagt ttgccactaa ctccaaatgg
    13501 aaagattgac atcaaaggat tgattaacga ggtgaataag agatgatgga gttttttcaa
    13561 cagcttcctc atttagagcc atatggcaat cctcagtatt ttgtttatgt gattgctgca
    13621 accttgccca tctttatagg tctctttttc aagaaacgct ttgcctggta tgaagtgttg
    13681 gtaagtctct tctttattgt caccatgttg gtgggtggaa agaccaatca actagctgcc
    13741 ttgggtattt acctttgctg ggaaatattg ctcctgcttt tctacaagca ttatcgaaaa
    13801 agcaaggatg gcaagtgggt cttctactta gttagttttc tgtccctact tccgattatc
    13861 tttgtcaagg tgcaaccagc tatcaatgga acgcagtctt tgcttgggtt cttgggaatt
    13921 tcttacctga cctttcgttc ggttgggatt atcatcgagc tgagagatgg agtgattaag
    13981 gattttaccc tctgggaatt cctccgtttc cttctcttca tgccaacttt ctcgagtggt
    14041 ccaatcgatc gctttaagcg atttaatgaa aattatcagg ccattcctga gcgagatgag
    14101 ttgatggata tgctggatga atctgtccgc tatatcatgc ggggcttttt gtataagttt
    14161 atcctagctc atgttttagg agagacctta ctccctcctc tgaagaattt agccttgcag
    14221 tcaggtggct tctttaatct ctatgccttg gcagttatgt atacttttgg tctggagctc
    14281 ttctttgact ttgcaggtta ttctatgttt gctttggcca tctcaaactt gatgggaatc
    14341 cgtagcccta tcaactttaa caagcccttt ttatcaaggg atttaaagga gttttggaat
    14401 cgctggcata tgagtctgtc cttctggttc cgtgactttg tctttatgcg aatggtgatg
    14461 gtgttaacca gaaagaaagt ctttaaaaat cgtaatgtaa cctcaagcat ggcctacatt
    14521 gtaaatatgc tgattatggg attttggcat ggcgtgacct ggtactatat cgcctatgga
    14581 ctctttcatg gactaggctt ggtcatcaat gatgcctggg ttcgcaagaa aaaaacgctc
    14641 aataaggaac ggaaaaaagc agggaaggct gccctacctg agaatcgctg gattcagttt
    14701 cttggcatgg ttgtcacttt ccatgttgtc atgttgtcat tcttaatctt ttctggattc
    14761 ttgaataatc tatggtttaa aaaataaaag gaaataaaat atggatatca aatcagaagt
    14821 tattgaaatt attgatgagt tgtttatgga agatgtttct gacatgatgg atgaagatct
    14881 ttttgatgca ggtgtcttgg atagtatggg aacggttgag ttgattgtgg agattgagaa
    14941 ccgttttgac attcgtgtcc ctgtaacaga gtttggtcgt gacgactgga atacagctaa
    15001 taaaatcata gctggtattg tggagctaca aaatgcttaa acgcttatgg atgatcttcg
    15061 gaccggtctt gatcgctggt ttgttggttt ttctgctcat tttcttttat cctactgaga
    15121 tgcatcataa tctaggagct gaaaagcgtt cagcagtggc tactactatc gatagtttta
    15181 aggagcgaag tcaaaaagtc agagcactat ctgatccaaa tgtgcgtttt gttcccttct
    15241 ttggctctag tgaatggctt cgttttgacg gtgctcatcc tgcggtatta gctgagaaat
    15301 acaatcgttc ctaccgtcct tatcttttag gacagggggg agctgcatcg cttaaccaat
    15361 attttggaat gcaacagatg ttaccacagc tggagaataa acaagttgtg tatgttatct
    15421 cacctcagtg gttcagtaaa aatggctatg atccagcagc cttccagcag tattttaatg
    15481 gagaccagtt gactagtttt ctgaaacatc aatctgggga tcaggctagt caatatgcag
    15541 cgactcgctt actgcaacag ttcccaaacg tagctatgaa ggacctggtt cagaagttgg
    15601 caagtaaaga agaattgtcg acagcagaca atgaaatgat tgaattattg gctcgtttta
    15661 atgaacgcca agcctccttt tttggtcagt tttcggttag aggctatgtt aactacgata
    15721 agcatgtagc taagtattta aaaatcttgc cagaccagtt ttcttatcaa gcaatagaag
    15781 atgttgtcaa agcagatgct gaaaaaaata cttccaataa tgagatggga atggaaaatt
    15841 atttctataa tgagcagatc aagaaggatt tgaagaaatt aaaggattct cagaaaagct
    15901 ttacctatct caagtcgcca gagtataatg acttgcagtt ggttttaaca cagttttcta
    15961 aatctaaggt aaacccgatt tttatcattc cacctgttaa taaaaaatgg atggactatg
    16021 ctggtctacg agaggatatg taccaacaaa cggtgcagaa gattcgctac cagttagaaa
    16081 gtcaaggttt taccaatata gcagattttt ctaaggacgg cggggagcct ttctttatga
    16141 aggacaccat tcaccttggt tggttgggtt ggttggcttt tgacaaggca gttgatcctt
    16201 tcctatccaa tcccacacca gctccgactt accatctgaa tgagcgcttt ttcagcaaag
    16261 attgggcgac ttatgatgga gatgtcaaag aatttcaata gataatataa tatagaagag
    16321 ttcaagaatg aaactagttt tcacgttttt tcttgactct ttttttgctt gtgatataat
    16381 taactggtaa acaaaatgtc aaagaatagt atttttctct taaaaaagag aagtctaaaa
    16441 ggaaaggagt cagatatgag acagctagca aaggatatca atgctttttt gaatgaggtg
    16501 attttgcagg cggaaaatca gcatgaaatc ctaataggtc attgcactag cgaggtggcc
    16561 ctgaccaata ctcaggagca tatccttatg ctcttgtcag aggaatcttt aacaaattca
    16621 gaattggccc gtcgtctcaa tgtcagtcag gcggcagtta ccaaggccat taagtctttg
    16681 gtcaaggaag ggatgttgga aacatctaaa gattctaaag atgcgcgtgt gattttttat
    16741 cagttgactg acttggctcg tccaatcgct gaggagcacc accatcacca tgagcataca
    16801 cttttaacct atgaacaagt ggcgactcag tttactccaa atgaacaaaa agtgattcag
    16861 cggtttttga ctgctttagt aggagaaatc aaataatgag atatattacg gtagaggatt
    16921 tgtccttcta ttatgataag gagcctgttc ttgaacatat caattattgt gttgatagtg
    16981 gggaatttgt taccttgact ggggaaaatg gagcggctaa gacgacgctc atcaaggcta
    17041 gtcttggaat tctgcaacca cgcattggaa aggtggctat ttcaaagaca aatacgcaag
    17101 gtaagaaatt gagaatagcc tatcttcctc aacaaattgc cagttttaat gctggttttc
    17161 caagtacggt ctatgaattt gtcaagtcgg gtcgctatcc gagaaaaggc tggttccgtc
    17221 gtttgaatgc tcatgatgag gagcatatca aggctagtct ggactcagtt ggcatgtggg
    17281 aacatcgaga caaacgcttg gggtctctat ctgggggaca aaagcagcga gcggtaattg
    17341 cgcgtatgtt tgcttctgac cctgatgtgt ttatcctaga cgagccgaca acggggatgg
    17401 atgcaggaag taaaaatgaa ttttacgaac tcatgcacca cagcgcccat catcatggca
    17461 aggctgtttt gatgattacc catgaccctg aagaagttaa ggattatgcg gatcgcaata
    17521 ttcatctagt ccgtaaccaa gactcgccat ggcgttgttt caatgttcat gagaatggcc
    17581 aggaggtggg ccatgcttag tttattatct tacgacttta tacaacgcgc ctttttggcg
    17641 gttattgcta tgagtctttt ctcaccggta ttgggaacct tcctcatctt gcgtcgtcag
    17701 agtttgatga gtgataccct tagccacgtc tcactttcag gtgtagcctt tggtctggtt
    17761 ttggggattt ctccaactgt ttctactatt gccattgtct tgattgcggc ggtctttctg
    17821 gagtatctcc gtacggttta caagagcttt atggaaatcg ggacagctat cctcatgtca
    17881 acaggtctgg ctgtttctct gattgtcatg agcaagggta aaagctcgag ttcaatgagt
    17941 ttggaccaat atctctttgg ttcgatcgtg actatcagtg aagaacaggt catttccctc
    18001 tttgtcattg cggcggttgt tttgattttg acctttctct ttcttcgtcc tatgtatatc
    18061 ttaacttttg acgaagatac ggcctttgtg gatggcttgc cagttcgtac catgtccatt
    18121 ctttttaaca tggtgacagg ggtggctatt gcccttatga ttcctgcagc aggagctctt
    18181 ctggtatcga ccattatggt cttgccagct agtattgccc tgcgtctggg gaaaaacttt
    18241 aaatcggtta tgctgcttgc cagtgcgatt ggctttttgg gaatggtagc aggactttac
    18301 atttcctact atgcagaaac acctgcaagt gcaagtatta ccattatttt tgtaactgtc
    18361 tttatactaa tcagtttagt aagacgtttt atcaaatagg agacgaacgt gaaaaaaatt
    18421 agcttattac tagccagtct atgtgccttg tttttagtgg cttgttccaa tcaaaaacag
    18481 gcagatggta aactcaatat cgtgacaacc ttttaccctg tctatgaatt taccaagcaa
    18541 gtcgcaggag atacggctaa tgtagaactc ctaatcggtg ctgggacaga acctcatgaa
    18601 tacgaaccat ctgccaaggc agttgccaaa atccaagatg cagatacctt cgtttatgaa
    18661 aatgaaaaca tggaaacatg ggtacctaaa ttgctagata ccttggataa gaaaaaagtg
    18721 aaaaccatca aggcgacagg cgatatgttg ctcttgccag gtggcgagga agaagaggga
    18781 gaccatgacc atggagaaga aggtcatcac catgagtttg atccccatgt ttggttatca
    18841 ccagttcgtg ccattaaact agtagagcac attcgcgaca gcttgtcagc agattatcct
    18901 gataaaaaag agacctttga gaagaatgca gctgcctata tcgaaaaatt gcaagccttg
    18961 gataaggctt acgcagaagg cttgtctcaa gcaaaacaaa agagctttgt gactcaacac
    19021 gcagccttta actatcttgc cttggactat ggactcaaac aagtcgcaat ctcaggcctt
    19081 tctccagatg cagagccatc agctgctcgc ttggcagaat tgacagagta tgtcaagaaa
    19141 aataaaatcg cctatatcta ttttgaagaa aatgcctcac aagcccttgc taacacactt
    19201 tcaaaagaag caggtgtcaa aactgatgtc ctcaatcctt tagaaagtct gacagaagag
    19261 gacaccaagg ctggagaaaa ctacatttcc gtgatggaga aaaacctcaa ggctttgaaa
    19321 caaacaacag accaagaagg cccagcaatc gaacctgaaa aggcagagga taccaagaca
    19381 gtccaaaatg gttacttcga ggatgcagct gtcaaggacc gcaccttgag tgactatgca
    19441 ggtaactggc aatcagttta tcctttcctt gaagacggca cgtttgacca agtctttgac
    19501 tacaaggcta agttgactgg taagatgacc caggctgagt acaaggctta ctatacaaaa
    19561 ggctatcaga cagatgtgac taagattaac attactgata atactatgga atttgttcaa
    19621 ggtggacaaa gcaagaaata cacttacaag tatgtcggta agaaaatttt gacttacaag
    19681 aaaggcaatc gtggcgtgcg tttcctcttt gaagccacag atgctgacgc tggacaattc
    19741 aagtatgttc agtttagtga ccacaatatc gccccagtta aggcagaaca tttccatatc
    19801 ttctttggag gcacaagcca agaagccctc tttgaagaaa tggacaactg gccaacctac
    19861 tacccagata acctatctgg ccaagaaatc gcccaagaaa tgttggcgca ttgatgagaa
    19921 atcgactagt tcataagagc tagtcggttt tttgatacta gatggctaac ttcattatag
    19981 acctttcaga actaagggaa cctgaaaaaa taaataaaac tcttgaaata tctggactta
    20041 tatgctagaa tgtagttagt gtataaggtt cattagaaca ccaaagcccc taactagtgc
    20101 catagttaag ggcttttttg acgtatctta gcgatttaat gggtgtttat aggctagtca
    20161 gtcggtctac ttcttaccat ctagccattt gcagacgaaa tacaagataa ttccagctat
    20221 aacatctgct ataatagtaa gagcaagctc tggtataagg ctcattagtt tcacctcctc
    20281 tcacgaaccc ataggaacgt aatcggtaac cgatgacaaa aatagtatac cacactaaat
    20341 ttagatcatc aacctcactt aatgcttgaa ctattgtaga ttatatgcta aaatgtagtt
    20401 aattgataag gttcattaga acaccaaagc ccctaactat ggccgtagtt aagggctttt
    20461 ttgacgtatc ttagcgattt aacgggtgtt tataggctag tcagtcggtc tactccttac
    20521 tatctagcca tctgcagacg aaatacaaga taattccagc tataacatcc gctataatag
    20581 taagagcgag ctctatgata aggctcatta gtttcacctc ctctcacgaa cccataggaa
    20641 cgtaatcggt aaccgatgac aaaaatagta taccacaata aatttagatc atcaaggtca
    20701 cttaattctt gaaatatagg gtctaggcga caaagtcttt aaaatcagaa aaacgcataa
    20761 tatcaggtgt gcaaaaactt gatactatgc gttttattgt gggaaggttt actccatttt
    20821 ctcctgaaat tgagtttttg tccagcctct gtttttgggg ttgctaagaa aataatgtca
    20881 tgtggtgaat atttgtaaat cagtcagcag acagaacgaa tactcttcga aaatctcttc
    20941 acatcatgtc agcttcgtct ttccgtatat atgtgactga cttcatcagt tctatctaca
    21001 acctcaaaac agtgtttcga gctgacttca tcagttttca aatctgtact ttgagcaagc
    21061 tgagactagc ttcctatttg attttcattg aatatcagaa acccattctc catcaaataa
    21121 ttcgactgcg tctaataatt tttggtctgg caaggtgtca gaaataaagg ttgtgtattt
    21181 ggagagggga ttaattttaa aaaatccagt cttgtaaaat ttagaactat caatcagtaa
    21241 gatggtttca tgggctttgt caataatatt cttttttgaa atagcttggc tgagagaagc
    21301 ttcataaaca tattggtcat caatacctct tgctgaacaa aatgctaaat cgatattaaa
    21361 atgatctaat aaagaatttt ccttatcata gttgactacg gaacaggatt gatgtttgac
    21421 ctcgccagat gtgataaaga ttttggagct atctttaaca gtttcagata gggtttgtgc
    21481 agtatgtaaa ccatttgtaa aaataatcaa attatcaagt tcagaaagat agggacagag
    21541 ttcgtagaca gtcgtactgg aatcaagata aatacacatg cctgaccgaa taaagtcttt
    21601 agcgagacta gcgattagtc ttttttgcct agtactttct ccttcccgta tttgatgaga
    21661 aagttcaatt gtgttcatag aggacagggt cacgtatccg tgctttcttt tgataagacc
    21721 ttgattttct aagaaaatta aatcacgacg taaggtactt gtgctggaga aagtgatttc
    21781 tgccagctct tttacggcaa ttcttttttt ctttttgatg atttcaatca attcaagtac
    21841 acgttcatct tttatcataa gctcctccta atttatcatt tcaactatat tatagcacaa
    21901 attggaggaa tttgaattat ttttatgaat attgggttaa catttgaaca ttattcaagt
    21961 aagcgttcac atattgaaaa aataaaacgt ggggattata ataaagttaa tcaaggacga
    22021 agagagaaga aaaatggaag cggttttagc aatagattta ggtgcgactt ctggaagagc
    22081 aatcgttggt tacctttctg aaaataaact agtaatggaa gaaataaatc gcttttctaa
    22141 tctacctatt agagtaaaag ggcatttatc ttgggatatt gactttctac tagctaaaat
    22201 tcttgaaagt atccgcttgg ctaatactag ttacaagatt ttatctatcg gtattgacac
    22261 atggggagtt gattttggac tggttgataa tgaaggtaag ctgttattac aacctgttca
    22321 ttatcgtgat gaaagaacaa agggagtgtt aaaggaaata tctgaaatga ctgaattaga
    22381 aaaactgtat tcagagacag gaaatcagat tatggagata aataccttgt ttcaactctt
    22441 taaggcacgt caagaatctc ctgactcttt ctataagacc aataagattc ttttaatgcc
    22501 agatttgttt aattatctct tgacaggtaa gtttgctaca gaaaaaagca ttgcttcaac
    22561 aactcaatta tttgatccta ggagtcaaaa ctggaatcag aatatcttaa aactatttga
    22621 attggattca tctttacttc ctgagattgt ttcagaggga aatgttcttg gaaggataaa
    22681 aaaggagtat ggtttaggcg atattcctgt tgtgaatgtt tgtagtcatg atacagcaag
    22741 cgcgattgtc tcagtaccta agacagaagg tagtttattt atttcatcag gtacttggtc
    22801 tttggttgga gtggaactta cttcaccgct tcttactacc gaatccttca gttatggatt
    22861 tacaaatgaa gtcggtaaag atggagtgat tacatttctg aagaattgta cagggttgtg
    22921 gatcatagag gaactaagac gttcatttga acgaagaggg aaagcctatt cttttgatga
    22981 tattaggaca atggtggaga aagaaaaaga aaatcttcct ctgattgata ctgaatcaac
    23041 tgaatttgca acagaatctg atatgcacaa gactttgaca gaatatctag cttatcatca
    23101 tgaaactaga gagtggacag atggacaact atttaagatt gtttatgaaa gcctagctga
    23161 aacgtatagg aaagcgatag agttactaga agaactaact cataaggttt ataagaggat
    23221 atatgtgatt ggaggaggtg ctagagccag ttactttaac caaatgattg ctgatagaac
    23281 tggtaaagag gttcttacag gtttgactga ggctacagct gtggggaata ttgttgtgca
    23341 gctcatagct atgggacaat taaaagggat ggaagaagct caccatgtta ttgaggagtt
    23401 tctacaatta gagagttatt actcccaaaa gaattaaaaa gattgagagt ttgtaaattt
    23461 gcctccctcc cccttcttaa gttttgtaca agaagggggg ataattggtg aattgaaaaa
    23521 tatttagtgt tttgatatga ggaggacaag aatgtcagat gtaaaacaag aattaattaa
    23581 gtatggcaag aagctagtag aaacagattt gacgaaagga acaggtggga atctcagtgt
    23641 tttcgatcgt gaaaaacaat tgatggcaat taccccgtcg ggtattgatt tctttgaaat
    23701 caaagaatcc gatattgtag tgatggatat taatggaaat gttgtagagg gagaacgctt
    23761 gccatctagc gaatggtata tgcatttgat tcaatatcaa actcgtgatg atatcgatgc
    23821 aattatccat gctcatacaa cttatgcaac agtattagct tgtctcagag aaccacttcc
    23881 ggctagtcat tatatgattg cagtggcagg gaaagatgtt cgggtagctg agtatgcaac
    23941 atatggtacg aaagaattgg ctgtgaatgc agctaaagca atggaaggtc gtagagcagt
    24001 tttactagcg aatcatggaa ttttagcagg tgcacaaaat ttattgaatg catttaatat
    24061 tgttgaagaa gttgaatatt gtgcaaaaat ttattgttta gctaagaatt ttggagagcc
    24121 agtagttctt cctgatgagg agatggattt aatggcagaa aaatttaaaa catacggtca
    24181 gagaaaatag ggaggatatt aatgttaaaa catataccga aaaatatttc tccagattta
    24241 ttgaagactt taatggaaat gggacatgga gatgaaatag tattagctga cgcgaattat
    24301 ccttctgcct catgtgcaaa taagctaatt cgttgtgatg gcgtaaatat tccagaatta
    24361 ttagattcca ttctgtattt aatgccatta gatagttacg tcgatagttc aattcagttt
    24421 atgaacgttg tttcgggtga tgatattcct aagatatggg gtacctatag acagatgatt
    24481 gaaggtcatg gtacagatct taaaacgatt acttatctta gaagagaaga cttttatgaa
    24541 cgtagtaaga aagcttatgc tattgttgct acaggagaaa cttcacttta tgctaatatt
    24601 atccttaaga aaggagtagt tgttgaaaga gaaaatgttt aatagaggaa ttttagttgc
    24661 cagtcatggt aattttgcta gcggagctct catgaccgca gaaatgtttg ttggtgagac
    24721 aacaaatgat agagttagga cattaggttt gatgcctgga gagaatattg tagagtttga
    24781 acattatttt aaaaatcaag taaatgaact gttagactca aatcaagagg ttatcgtttt
    24841 gactgacttg attggaggaa gtcctaataa tgtggctttg tcacggtttt taaatttgga
    24901 ttcagttgat attgtaacag ggtttaatat ccctctccta gtggaattaa tatcaagtta
    24961 tgattcaaaa atcaatttag aagaaattgt tcaaaatgct caaaatagtt tgtttaatgt
    25021 taaacaacaa cttaacgtag aggaggaaga ggatttatgt ctatagagtt tgttcgtatt
    25081 gatgaccgtc tggtacatgg tcaagttgtc actacgtggc taaaaaagta tgatattgag
    25141 caagttatca ttgttaatga tcgcatctca gaagataaaa cacgacaatc tattttaaag
    25201 atttctgcac cggtaggttt aaaaattgtt ttctttagtg taaaacggtt tgtggaagtt
    25261 ttaaactctg tgccaataaa aaagagaaca atgctgatat atacaaatcc aaaagatgtg
    25321 tatgattcta ttgaaggaaa tttaaaattg gagtacctca atgtaggaca gatgagtaaa
    25381 acggaggaaa atgaaaaggt aacgggaggt gtagctctag gtgaagaaga caaatattat
    25441 tttaagaaaa tagttgataa gggaacgaga gttgaaattc aaatggttcc taatgataaa
    25501 gttacaatgt tggaaaaatt tttataaaaa taatttaagg aggtacagta tatgctattc
    25561 acacaagcat tactggtgac attagttggg attattgcca ctattgacta taatggaccg
    25621 ttatttatga ttcaccgtcc gttagttaca agtgcaatgg ttggcttagt cttaggagat
    25681 ttcacccaag gtgttcttat tggttcagct cttgaattaa cttggctcgg tgtaacaggt
    25741 attggaggtt acactccacc agatactatt tcaggtgcga ttattggtac tgcatttggt
    25801 attttatctg gtcaaggaga aactgctggt atcgctatag cagttccaat tgcagttgct
    25861 acccaacagt tggatgttct tgcaaaaact ttagatgttt attttgtgaa aaaagctgat
    25921 aatgatgcta aaaacggaga ttattcaaag atcggttttt atcattattc aagtttggtt
    25981 ttaatcacgt tatttaaaat tgtaccaatt ttcctagcta ttatgcttgg aggggaatat
    26041 gtggcagact tgtttgctaa ggttccacca atcgttatgc agggacttaa ctctgcaggt
    26101 gctttactac cttcaattgg ttttggtatg cttttaaata tgatgctcaa gaaaaatatg
    26161 tgggtattct tgttgattgg attcatttgt tctgtgtatg gaggaatgtc aaccattggg
    26221 atctcactag ttggtattgc ggtagcatac ttctacgata tgattggaag taaaccacaa
    26281 gaaacaactt caagtagtga tgttgaggag gatcttgatc tatgatgaat aataaagtaa
    26341 ctaaagttga acttaaaaaa gttttcaaac gaagttttat gtatggttct tcatggaact
    26401 atgagagaat gcagaaccta ggttttctat atacaattct tccagtattg aaaaaactat
    26461 acccagacaa agattcagct tctcctgcaa tgaaacgtca ccttgagttt ttcaatactc
    26521 atcaaacagc ggcaccattt attcttggag ttacttccgc tatggaagaa caagaaggaa
    26581 atgaaggtgc agcttcaatt actggtatta aagttggctt gatggggcca ctggctggtc
    26641 taggagatag tttgttctgg ctgacactag ttcctatctg ttttagtatt ggtgcgtctt
    26701 attctaaaga cggcggtgct ttaggtatct ttatcgcctt aatattgttt aatattatta
    26761 atattcctgt taaatatttc ggtttgaaat atgggtatac taagggttct agtcttatcc
    26821 aagaaaataa tacaaaagga acattgaatc gcgttacgag tatggcgaca gcattagggc
    26881 tagtactagt gggtggtttg attccatcaa tggttggtat taattttgga ttagaattta
    26941 agcaggggga acttgttatt tctgttcaag aaatgattac aaaattaatt ccaggattta
    27001 tccctatggc tttgacttta ttaatgtgta aattaattag aaaaggaaag aatccggttg
    27061 tactaatctt tagtgttatg gctattggag ttatcctagt tgttttagga attttgaagt
    27121 agtagaaagt gtggaggtgg tatttgggat atcacctcca ttttggaaga gaggtaaaga
    27181 gtgaaattat ggtataagaa agctgcctca aattggaatg aagccttgcc gattgggaac
    27241 ggtcatttag gtggtatgat ttatggttca gctacaaaag aatgtattca actaaacgat
    27301 gagactattt ggtatagagg aaagtcagat agaaataatc cagactcact attgcatctt
    27361 aaaaaaattc gggaatatct tttagatgga gaaattcaga aagccgaaga attgataaag
    27421 ttaacaatgt ttgctacccc aagagatcaa agccactatg aattacttgg ggaactttac
    27481 attgagcata tagatattca gtcttgtgct ctttcattgt atgaaagaga gctagattta
    27541 gatacagcta tttctaatgt tgtgtttgag cctaatagtt gtaatttaca aataaaaaga
    27601 gaatatttta cgagttttaa taagaatatt ttatgttgcc gtatagtgtc atcagttcaa
    27661 aacacattaa atttaaacat taatttgggt agaaataaac ggtttaatga tgaagtatct
    27721 aaactggatt caagtacaat tttaatgtcg gcctctgctg gaggtagaaa aggtgttcag
    27781 tttaaagtag tatgtcattc taaggttacg gatggtgaag taagtgtatt gggagagaca
    27841 atagttattc ggaatgctac agaggtattt ctttatctca aatcaatgac ggattattgg
    27901 ggaaatatag atatttcttc tcttcaggga gaatttagta gtattgatta ctttacagaa
    27961 aaagatgaac atgtaaaaaa atatcaggag caatttaata gagttgattt taaactagac
    28021 tatagtaaag attgtcttag cattccaacg aatctacttc ttgaaaacac taaaaagtat
    28081 agtaactact tgactaactt gttatttcat tatggaagat atctgttaat atcgtctagt
    28141 caaccgaatg gtttacctgc caatcttcaa ggaatatggt gtgatgaatt aaatccaatt
    28201 tggggttcta aatatacgat taatattaat actcaaatga attattggat ggtaggtcca
    28261 tgtgatttac cagaagtaga atatccatta tttgatatgc tcgaaagaat gagagaaccg
    28321 ggaagactaa ccgctaagaa aatgtatgga gctagaggtt ttacagcaca tcataatacg
    28381 gatggttttg gcgatacggc tccccaatct catgccatgg gggctgcaat ttgggtatta
    28441 actattccat ggttatgtac tcatatttgg gaacactatt tatatttcca agatgagcgt
    28501 attcttacgg aacattttga aatgataaaa gaagcatttc ttttctttga agattattta
    28561 tttgaggtgg atggctactt gatgacaggt ccaagtgtct caccggaaaa taaatatcgc
    28621 ttaaaaaatg gtattgaagg aaatgcttgt ctatcatcta caattgataa tcaaattcta
    28681 agatattttt gtgattcatg cattggcatt gcaaaacaat taggagacaa ttcggatttt
    28741 attagtcgtg tgaaggagtt aaaaaagaaa ctacctaaaa caaaaatagg tagtaatggg
    28801 caaatccaag aatggttaga agattatgaa gaagtagagc ctgggcatag acacatttca
    28861 cctctatttg ggctttatcc ttataatgag attgatattc ataaaactcc ggaattagca
    28921 gaagcagcta aaatcactat caataggaga ttatcaaacg ctaatttttt atcttcacag
    28981 gagagggagc aagcgattaa taattggtta gtaagtggtt tgcatgctag tacacaaaca
    29041 ggttggagtg ctgcatggct gattcatttt tttgcgagac tatatcaagg tgaacctgct
    29101 tataaccaga ttaatggttt gttaaataat gcgactcttg gcaatttatt tcttgaccat
    29161 ccaccatttc aaattgatgg taatttaggt ttggtgagtg gaatttgtga attattagta
    29221 cagagccatc ataattggtt atcactaatt ccagctttac cttctgcttg gtcagaagga
    29281 gaagtgaaag gtttcagagt aagaggagga tataaggtat cgtttgcttg gaaaaatggg
    29341 gatataacat tcctaaaatt ggaaggagga aacaaagatc aaaaagtaag agtaagaata
    29401 tatggcaaaa atactgatgt acaaaatatt gaattggtat ttaattcaga aaaaattatt
    29461 gagttaaatt tttaggtata agtcatgaat aaagaaaaaa taaaaagaaa attaatcaca
    29521 atattgtttg tatgtattgg gatgttatgt tttggattgt tagcaggagt taaggctgat
    29581 aatcgtgttc aaatgagaac gacgattaat aatgaatcgc cattgttgct ttctccgttg
    29641 tatggcaatg ataatggtaa cggattatgg tgggggaaca cattgaaggg agcatgggaa
    29701 gctattcctg aagatgtaaa gccatatgca gcgattgaac ttcatcctgc aaaagtctgt
    29761 aaaccaacaa gttgtattcc acgagatacg aaagaattga gagaatggta tgtcaagatg
    29821 ttggaggaag ctcaaagtct aaacattcca gttttcttgg ttattatgtc ggctggagag
    29881 cgtaatacag ttcctccaga gtggttagat gaacaattcc aaaagtatag tgtgttaaaa
    29941 ggtgttttaa atattgagaa ttattggatt tacaataacc agttagctcc gcatagtgct
    30001 aaatatttgg aagtttgtgc caaatatgga gcgcatttta tctggcatga tcatgaaaaa
    30061 tggttctggg aaactattat gaatgatccg acattctttg aagcgagtca aaaatatcat
    30121 aaaaatttgg tgttggcaac taaaaatacg ccaataagag atgatgcggg tacagattct
    30181 atcgttagtg gattttggtt gagtggctta tgtgataact ggggctcatc aacagataca
    30241 tggaaatggt gggaaaaaca ttatacaaac acatttgaaa ctggaagagc tagggatatg
    30301 agatcctatg catcggaacc agaatcaatg attgctatgg aaatgatgaa tgtatatact
    30361 gggggaggca cagtttataa tttcgaatgt gccgcgtata catttatgac aaatgatgta
    30421 ccaactccag catttactaa aggtattatt cctttcttta gacatgctat acaaaatcca
    30481 gctccaagta aggaagaagt tgtaaataga acaaaagctg tattttggaa tggagaaggt
    30541 aggattagtt cattaaacgg attttatcaa ggactttatt cgaatgatga aacaatgcct
    30601 ttatataata atgggagata tcatattctt cctgtaatac atgagaaaat tgataaggaa
    30661 aagatttcat ctatattccc taatgcaaaa attttgacta aaaatagtga ggaattgtct
    30721 agtaaagtca actatttaaa ctcgctttat ccaaaacttt atgaaggaga tgggtatgct
    30781 cagcgtgtag gtaattcctg gtatatttat aatagtaatg ctaatatcaa taaaaatcag
    30841 caagtaatgt tgcctatgta tactaataat acaaagtcgt tatcgttaga tttgacgcca
    30901 catacttacg ctgttgttaa agaaaatcca aataatttac atattttatt gaataattac
    30961 aggacagata agacagctat gtgggcatta tcaggaaatt ttgatgcatc aaaaagttgg
    31021 aagaaagaag aattagagtt agcgaactgg ataagcaaaa attattccat caatcctgta
    31081 gataatgact ttaggacaac aacacttaca ttaaaagggc atactggtca taaacctcag
    31141 ataaatataa gtggcgataa aaatcattat acttatacag aaaattggga tgagaatacc
    31201 catgtttata ccattacggt taatcataat ggaatggtag agatgtctat aaatactgag
    31261 gggacaggtc cagtctcttt cccaacacca gataaattta atgatggtaa tttgaatata
    31321 gcatatgcaa aaccaacaac acaaagttct gtagattaca atggagaccc taatagagct
    31381 gtggatggta acagaaatgg taattttaac tctggttcgg taacacacac tagggcagat
    31441 aatccctctt ggtgggaagt cgatttgaaa aaaatggata aagttgggct tgttaaaatt
    31501 tataatcgca cagatgctga gactcaacgt ctatctaatt ttgatgtgat tctatatgac
    31561 aataatagaa acgaagttgc taagaaacat gttaataatt tgtcgggtga atctgttagt
    31621 ctagatttca aagaaaaagg agcaaggtat attaaagtta aattactaac gagtggagtg
    31681 cctttgagtt tagcagaagt agaggttttt agagaatcag atggtaagca atctgaagag
    31741 gatatagata aaataacaga agataaagta gtctctacaa ataaggtagc tactcaaagt
    31801 tcaaccaatt atgagggtgt agctgcttta gcagttgatg gtaataaaga tggagattac
    31861 ggacatcatt cggtgactca tactaaggca gattctaacg cttggtggca ggtcgatctg
    31921 ggagaagagt ttacggtttc taaagttgat atttataata gaacagatgc cgaacctcag
    31981 cgtttatcta attttgatgt tatttttcta tcttcatcag gagaagaagt ttttagaaga
    32041 cattttgata aagtagttga tggtttgtta tctttaaaag taccttctgt aggggctaag
    32101 ctagtcaaaa tagaattaaa atcagcagct attccgttaa gtttagcgga agttgaagtc
    32161 tatggttcaa agagaactcc gaagaaactt tctaatattg cattaacaaa agaaactcga
    32221 cagagttcaa cggattacaa tggtttttct cgtctagcag ttgatggaaa taaaaacgga
    32281 gattatggtc atcattcagt gactcatacc aaagaagatt ctccttcatg gtgggagata
    32341 gatttagcac aaaccgaaga attagaaaag ttaattattt ataatagaac agatgctgaa
    32401 attcagagat tatcaaattt tgatattatt atatatgatt caaatgatta tgaagttttt
    32461 acacaacata ttgacagttt agaaagcaat aatctatcca tagacttaaa aggactgaag
    32521 ggaaaaaagg ttagaatttc tttgagaaac gcaggaattc ctttaagttt agcagaggta
    32581 gaggtttata cttataagta attttaaaaa ttatcaccca ggctaccgta aatataatgg
    32641 agatggtagt atgaaagaaa cagaaaaata agaggaaaat agtatgattc aacatccacg
    32701 tattgggatt cgtccgacta ttgatggtcg tcgtcaaggt gtacgcgaat cacttgaagt
    32761 gcaaacaatg aacatggcta aaagtgtggc agatttgatt tcaagcacat tgaaatatcc
    32821 agatggggaa cctgtggaat gcgtgatttc tccatctact attggccgtg taccagaggc
    32881 tgcagcttcc catgagttgt ttaaaaaatc aaatgtttgc gcaacaatta cagttacacc
    32941 atgctggtgt tatggtagtg aaactatgga tatgtctcca gatattcctc atgctatttt
    33001 gggatttaat gggacagaac gcccaggagc tgtctatctt gcagctgtac tagcttcaca
    33061 tgctcaaaaa gggattccag cctttgggat ttatggaaga gatgttcagg aagctagtga
    33121 cacagatatt ccagaagatg tcaaagaaaa acttttacgc tatgcgcgtg cagctcttgc
    33181 aactggcttg atgagagaca ctgcttacct atcaatgggt agtgtttcga tggggattgg
    33241 tggttctatt gtaaatccgg atttcttcca agaataccta ggaatgcgaa atgaatcggt
    33301 agatatgacg gagttcacgc gccgtatgga ccgtagtatt tacgaccctg aagagttcga
    33361 acgtgcgctc aaatgggtga aagaaaacgt aaaagaagga ttcgaccata accgtgaaga
    33421 ccttgtttta agccgtgaag aaaaagatag acaatgggaa tttgttatta agatgttcat
    33481 gattggacgt gacttaatgg ttggtaaccc aagacttgct gagcttggtt ttgaggaaga
    33541 agcagttggt caccatgctt tagtagctgg tttccaaggt caacgccagt ggacagatca
    33601 tttcccaaat ggggacttta tggaaacttt cctcaatact cagtttgact ggaatggtat
    33661 tcgaaaacca tttgtatttg cgacagagaa tgattcacta aatggtgtgt ctatgctctt
    33721 taattatcta ttaacaaata ctccacaaat ctttgctgat gtgcgtactt attggagccc
    33781 agaggctgtt aaacgtgtaa cgggacatac tttagagggt cgtgctgcag ctggcttctt
    33841 acatctaatc aactctggtt cttgtacatt ggatggtaca ggtcaagcta ctcgagatgg
    33901 caaacctgtt atgaaaccat tctgggagtt ggaagaaagt gaagtgcagg ctatgcttga
    33961 aaatacagac ttcccaccag caaaccgcga atacttccgt ggaggaggat tctcaactcg
    34021 tttcttgacg aagggggata tgccagtaac aatggtacgt ctcaatcttt taaaaggggt
    34081 tggtccagtg ctacaaattg cagaaggtta tacacttgaa cttcctgaag atgttcacca
    34141 tactttagat aatcgtacag atccaggatg gccaactact tggtttgctc cacgtttgac
    34201 aggaaaaggt gctttcaagt ctgtctatga cgtcatgaat aattggggag ctaatcacgg
    34261 agccataaca tatggacaca ttggagcaga cttgattacc ttggcttcta tgttgagaat
    34321 tcctgtcaat atgcataatg tacctgagga agatatcttt agacctaaaa attggtcctt
    34381 atttggaaca gaagatctag aatcagcaga ctatcgtgca tgtcagttgt tgggaccact
    34441 acataaataa aacttgttta tataggaggt gaacttacgt ccctcctatc cttttaaaaa
    34501 gatttgttaa acaattcaca aataattgaa aacgaataca aaaagtaata taatgatgtt
    34561 aaatagatag cgcggaggcg caggaggaaa attatatggc tacattttat gttccggcag
    34621 tcaaccttat tggtaaaggt gttgtaaatg aagtgggtcc ttatatcaag gaacttggct
    34681 ataaaaaggc acttttggtt acagataagt acatcgaagg cagtgatatt ttgcctaagg
    34741 ttttaaaacc actggataca gaaggaatcg aatatgtcat ctttagcgat gtagagccaa
    34801 accctacttg taaaaatgtc acagatgggg tagctgcttt gcaagaacat ggctgtgact
    34861 ttatcatcag tcttggcggg ggttctccac aggatgcagc tagttgtatt tctatcatgg
    34921 ctacaaatgg tggaaaacca caggattatg aagggcttca taagtctgct aaaaaaggct
    34981 tgccagttgt ggctatcaat acaacggcag gaacttctgc agaaatcact attaactatg
    35041 tgattactga tgaagaacgc aaggttaaga tggtaatggt tgacaagaat agccttgctc
    35101 ttatctctgt taatgacccg gaactcatgc tttccaaacc taaaggcctg actgctgcta
    35161 ctggtatgga tgctctgact cacgctgttg aagctttggt aacacctggt gcttatgatg
    35221 taaccaagaa actgtctatt ggtgctattg agcttatcaa ggaatatctt cctcgtgctg
    35281 tagaaaatgg acatgatatt gaagcgcgtg aaggtatggt caatgccatc ttccttggtg
    35341 gtatgagctt taataatgct ggtcttggtt atgttcactc aatggctcac caactcggtg
    35401 cagtatataa tttgccacat ggcgtgtgct gtgccatgtt gctaccagtt atagaacgtg
    35461 aaaatgctaa acgtgtacca gaagctttcc gcaatgttgc caaagccttg ggacttcatg
    35521 tagaaggtaa atcagatcaa gaatgtgccg attatgcgat tgctgagatt gagaagcttt
    35581 ctgagacagt aggtattcct aagaaactga ctgaacttgg tattgaagaa aaagatttcg
    35641 actttgaata cctttctaag aatgccttga ttgatgcctg cgcaccagga aatccattta
    35701 tgccaacctt agaagaaacg attgcctttt ataaagagtt attttagcaa gagattggac
    35761 gacaatagga caattcacct ctaattattg acatttcagg aaaaatcgca gtaaaaacgg
    35821 cacagccttc tttaaatgtg ctataatact agaaaaatac ttgtggaggt tccattatgg
    35881 caatattttt catgattttt ctgattgttt gtgtgctcct attggtgata gtcacactga
    35941 gtacagttta tgtggttcgt cagcagtcgg tggcgattat tgaacgcttt gggaaatacc
    36001 aaaaggttgc taatagcggt attcatattc gcttgccttt tgggattgac tcgattgcag
    36061 cacggattca gttgcgcttg ttgcaaagtg atattgtggt tgagactaag accaaggaca
    36121 atgtgttcgt tatgatgaat gtagcgactc agtaccgtgt caacgagcag agcgtgacag
    36181 atgcttacta taaactcata cgtccagaat ctcagattaa atcttatatc gaagatgctc
    36241 ttcgctcttc tgttccaaaa ttaaccttgg atgaattgtt tgagaaaaaa gatgagattg
    36301 cccttgaagt tcaacaccaa gtagcagaag aaatgaccac ttacggctac attatcgtga
    36361 aaaccttgat taccaaggtc gaaccagatg cagaagttaa gcaatccatg aatgaaatca
    36421 atgcggcgca acgtaagcgg gtcgcagcac aagaattggc ggaagctgac aagattaaaa
    36481 ttgtcactgc agctgaagca gaagcagaaa aagaccgcct tcatggtgtg gggattgccc
    36541 aacaacgtaa ggcgattgtg gatggattgg cagagtctat caccgaactc aaggaagcca
    36601 atgttggcat gacagaagaa caaatcatgt ctatcctctt gaccaaccag tatttggata
    36661 ccttgaatac ctttgcctct aaaggaaatc aaaccatctt tttaccaaat actccaaatg
    36721 gtgtggatga tatccgaaca caaatcttgt cagcccttcg cgctgagaag aaataataga
    36781 ctaatactct tcgaaaatct cttcaaacca cgtcagcgtc gtcttgccgt atagatgtta
    36841 ctgacttcgt cagttctatc tgcaacctca aaacagtgtt ttgagcaacc tgtggctagt
    36901 ttcctagttt gctctttgat tttcattgag tataaagagc ttggcaacag gctctttttg
    36961 tattgcgttt attaagaaaa acaaaaaaca tcgatataca tttaactaaa aattattgta
    37021 tgtcatctcc cttaattagg aatgataagg gaataactag aaagatttgt gaatacaaac
    37081 tatttctgat atacaaaata tacagtaata atgaatgatg ggagatggga tgaaagaatt
    37141 tcaatttgag agaaagcagc gtttttcttt gaggaaatat gcaataggag cttgttcggt
    37201 cttgctagga acgagtttat tttttgctgg tatgggtgct cagcctgtac aggctacaga
    37261 aacgagttca gcactaattt caagtcatta tttggatgag caggatttat ctgaaaagct
    37321 gaaatctgag ttgcaatggt ttgaattaga aaacaagctt ttgaacttat gggagcatta
    37381 ggttactatg aaggatttgt tccttatgtt tcaaatcaat acaaaaacca aactgaagaa
    37441 gaaggcaaac cgctatctga taaatatatt ttcgaaaaaa tcttaggaaa aacatatgca
    37501 gccttcaaaa aagatcaaat taatgagcgt gtcgagaaat taggtaagtt aaaacctatt
    37561 acaataaatt acaacggaaa atcagaagta attgatagta aagaaaaatt acaaaagctt
    37621 atgaataaag ccgttaaaga cgaagtggct caaatatagt aaaatgaaat aagaacagga
    37681 caaatcgatc aggacagtca aatcgatttc taaaaatgtt ttagaaggag aggtgtacta
    37741 ttctagtttc aatatactat aaaatcaggc aataccacgg ctaaaaagtt tgagtttatc
    37801 gaaacaccag tgcaaaaact gaaaaaagcg atttataaag ctcatctcaa agattcagat
//
LOCUS       DAVLVP010000010        35989 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.010, whole genome shotgun sequence.
ACCESSION   DAVLVP010000010 DAVLVP010000000
VERSION     DAVLVP010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35989
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.010"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            3..75
                     /locus_tag="VYN69_000488"
     tRNA            3..75
                     /locus_tag="VYN69_000488"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:36..38,aa:Val,seq:tac)
     gene            81..151
                     /locus_tag="VYN69_000489"
     tRNA            81..151
                     /locus_tag="VYN69_000489"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:113..115,aa:Gly,seq:tcc)
     gene            186..259
                     /locus_tag="VYN69_000490"
     tRNA            186..259
                     /locus_tag="VYN69_000490"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:220..222,aa:Ile,seq:gat)
     gene            343..432
                     /locus_tag="VYN69_000491"
     tRNA            343..432
                     /locus_tag="VYN69_000491"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:379..381,aa:Ser,seq:tga)
     gene            443..516
                     /locus_tag="VYN69_000492"
     tRNA            443..516
                     /locus_tag="VYN69_000492"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:477..479,aa:Met,seq:cat)
     gene            520..592
                     /locus_tag="VYN69_000493"
     tRNA            520..592
                     /locus_tag="VYN69_000493"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:553..555,aa:Phe,seq:gaa)
     gene            605..685
                     /locus_tag="VYN69_000494"
     tRNA            605..685
                     /locus_tag="VYN69_000494"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:639..641,aa:Tyr,seq:gta)
     gene            690..760
                     /locus_tag="VYN69_000495"
     tRNA            690..760
                     /locus_tag="VYN69_000495"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:722..724,aa:Trp,seq:cca)
     gene            775..847
                     /locus_tag="VYN69_000496"
     tRNA            775..847
                     /locus_tag="VYN69_000496"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:808..810,aa:His,seq:gtg)
     gene            858..929
                     /locus_tag="VYN69_000497"
     tRNA            858..929
                     /locus_tag="VYN69_000497"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:890..892,aa:Gln,seq:ttg)
     gene            939..1022
                     /locus_tag="VYN69_000498"
     tRNA            939..1022
                     /locus_tag="VYN69_000498"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:973..975,aa:Leu,seq:caa)
     gene            1129..2406
                     /locus_tag="VYN69_000499"
     CDS             1129..2406
                     /locus_tag="VYN69_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcysteine synthetase"
                     /protein_id="HEV1532817.1"
                     /translation="MSCSIDLLKHRYLKNIKENPELFVGIELEYPVASLEGDATDVEV
                     IKDLFHYLVSTLDLTVAKVDDFGNLIQLVDPISQDAILFEVSYTTIEFAFGKAETIQE
                     VENRFNNYMNVIQRKLAESNHAIVGCGIHPNWDKNENCPVAYPRYQMLMDYLNLSRNI
                     IKSDLHHFPEYGTFICGSQVQLDISKTNYLRVINAFTQIEAAKAYLFANSEFSGADWD
                     TKISRDIFWEESMHGIYPENVGVNARLLNDEADFFDYLNHSAIFTAERDGQTYYFYPI
                     QAGDYLATPEIQAFALNGDEVIIYPQEKDFETHRSYQYQDLTTRGTVEFRSVCTQPLD
                     RTFASAAFHLGLLVNLDKLEAYLETAPFFKVFGYDYKSLRRQFSKKNLTDEEETTIIE
                     FSKDLLLLAEEGLVVRNKEEMTYLQPLREELSL"
     gene            2482..3966
                     /locus_tag="VYN69_000500"
     CDS             2482..3966
                     /locus_tag="VYN69_000500"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006595200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HEV1532818.1"
                     /translation="MTLVYQSTRDANNTVTASQAILQGLATDGGLFTPVTYPKVDLDF
                     DKLKDASYQEVAKLVLSAFLDDFTAEELDYCINNAYDSKFDTPAIAPLVKLDGQYNLE
                     LFHGSTIAFKDMALSILPYFMTTAAKKHGLENKIVILTATSGDTGKAAMAGFADVPGT
                     EIIVFYPKGGVSKVQELQMTTQNGDNTHVIAIDGNFDDAQTNVKHMFNDVALRERLAA
                     NKLQFSSANSMNIGRLVPQIVYYVYAYAQLVKTGEIVAGDKINFTVPTGNFGNILAAF
                     YAKQIGLPVGKLICASNDNNVLTDFFKTRVYDKKREFKVTTSPSMDILVSSNLERLIF
                     HLLGNDAVKTAELMNALNTQGQYELTDFDAEILELFAAEYATEEETAAEIKRVYELDS
                     YIEDPHTAVASAVYRKYQAVTNDATKTVIASTASPYKFPVVAVEAVTGKAGLTDFEAL
                     AQLHEISGVAVPPAVDGLETAPVCHKTTVAAADMQVAVEAYLGL"
     gene            4027..5307
                     /locus_tag="VYN69_000501"
     CDS             4027..5307
                     /locus_tag="VYN69_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEV1532819.1"
                     /translation="MFKKNKDIFNIALPAMGENFLQMLMGMVDSYLVAHLGLIAISGV
                     SVAGNIITIYQAIFIALGAAISSVISKSIGQKDQSKLAYHVTEALKITLLLSFLLGFL
                     SIFAGKEMIELLGTERDVAESGGLYLSLVGGSIVLLGLMTSLGALIRATHNPRLPLYV
                     SFLSNALNILFSSLAIFVLDMGIAGVAWGTILSRLVGLVILWSQLKLPYGKPTFGLDK
                     ELLTLALPAAGERLMMRAGDVVIIALVVSFGTEAVAGNAIGEVLTQFNYMPAFGVATA
                     TVMLLARAVGEDDWKRVASLSKQTFWLSLFLMLPLSFSIYVLGVPLTHLYTTDSLAVE
                     ASVLVTLFSLLGTPMTTGTVIYTAVWQGLGNARLPFYATSIGMWCIRIGTGYLMGIVL
                     GWGLPGIWAGSLLDNGFRWLFLRYRYQRYMSLKG"
     gene            5311..5931
                     /locus_tag="VYN69_000502"
     CDS             5311..5931
                     /locus_tag="VYN69_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001172828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1532820.1"
                     /translation="MQKTAFIWDLDGTLLDSYEAILSGIEETFAQFSIPYDKEKVREF
                     IFKYSVQNLLVRVAEDRNLDVEVLNQVRAQSLAEKNAQVVLMPGAREVLAWADESGIQ
                     QFIYTHKGNNAFTILKDLGVESYFTEILTSQSGFVRKPSPEAATYLLDKYQLNSDNTY
                     YIGDRTLDVEFAQNSGIQSINFLESTYEGNHRIQALADISRIFETK"
     gene            6097..7275
                     /locus_tag="VYN69_000503"
     CDS             6097..7275
                     /locus_tag="VYN69_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1532821.1"
                     /translation="MKKRMLLASTVALSFAPVLATQAEEVLWTARSVEQIQNDLTKTD
                     NKTSYTVQYGDTLSTIAEALGVDVTVLANLNKITNMDLIFPETVLTTTVNEAEEVTEV
                     EIQTPQADSSEEVTTATADLTTNQVTVDDQTVQVADLSQPIAEAPKEVASSSEVTKTV
                     IASEEVAPSTGTSVPEEQTAETSSAVAEEAPQETTPAEKQETQVSSQTESAVEATTMP
                     VEEKATETTATSSEAKEVASSNGATAAVSTYQPEETKTISTTYEAPAAPDYAGLAVAK
                     SENAGLQPQTAAFKEEIANLFGITSFSGYRPGDSGDHGKGLAIDFMVPESSELGDKIA
                     EYAIQNMASRGISYIIWKQRFYAPFDSKYGPANTWNPMPDRGSVTENHYDHVHVSMNG
                     "
     gene            7789..8127
                     /locus_tag="VYN69_000504"
     CDS             7789..8127
                     /locus_tag="VYN69_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1532822.1"
                     /translation="MTRYQILLFLLEHSLCNQMAVQERLKIDQAALTRHFKILETEGL
                     VERHRNPENQREVLVEAAKYAKEQLVVNPPLQHIKVKEEMENILTEFERTELSRLLNK
                     LVLGIENIEI"
     gene            8138..8497
                     /locus_tag="VYN69_000505"
     CDS             8138..8497
                     /locus_tag="VYN69_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HEV1532823.1"
                     /translation="MSIILTTIVALEHFYIFYLESIATQSDATSRVFNMEKEELAHPS
                     VSSLFKNQGIYKDLLGVFLLYGIYFSQNLEIVTIFVLFVIGAATYGSLTADKKIILKQ
                     GGSAILALISILLFKYT"
     gene            8604..9248
                     /gene="pcp"
                     /locus_tag="VYN69_000506"
     CDS             8604..9248
                     /gene="pcp"
                     /locus_tag="VYN69_000506"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1532824.1"
                     /translation="MKVLVTGFEPFGGEKGNPALEAIKGLPAEIHGAEVRWLEVPTVF
                     HKSAQVLEEEMNRYQPDFVLCIGQAGGRTSLTPERVAINQDDARTSDNEDNQPIDRPI
                     RPDGASAYFSSLPIKAMVQAIKKEGLPASVSNTAGTFVCSHLMYQALYLVEKKFPYVK
                     AGFMHIPYMMEQVVNRQTTPAMSLVDIRRGIEAAIGAIIEHGDQELKLVGGETH"
     gene            complement(9276..10133)
                     /locus_tag="VYN69_000507"
     CDS             complement(9276..10133)
                     /locus_tag="VYN69_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEV1532825.1"
                     /translation="MNYPKIDLKTIRQESKHFQADTPRLFLLYILPSMLVILSSFLNP
                     LSRIHGTVLEQPFFSILGQILQTYLFPLLVSFIGTILLTSSVYATLTLMKDSKTEPSV
                     KNSLALFDEERFSQTFLTLLLKRFYLFLWSIPNLLGIYLLFYSSFLAKKFVTLHPEFP
                     NLDLSSVETERFLMVFGLYFLASLILIIVGNILYIPQYYAYSQVEFLLCYSLDLGQVS
                     PRRILKTSRSFMKGYKFQHFVLDLQLLPWYFLNWITFGIASFSLLPYIQCTKIMFYRA
                     VLARKRPKA"
     gene            10263..11405
                     /gene="tgt"
                     /locus_tag="VYN69_000508"
     CDS             10263..11405
                     /gene="tgt"
                     /locus_tag="VYN69_000508"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004298827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEV1532826.1"
                     /translation="MSDSPIKYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQAT
                     VKTQSPEELKEMGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQPILTDSGGFQV
                     YSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQPYD
                     YVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAHDLVSMDFSGYSI
                     GGLAVGETHEEMNAVLDFTTQLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAQFAEDFTPLDPECDCYTCNNYTRAYLRHLLKADETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREYFVEKYGYNKSGRNF"
     gene            11503..13320
                     /locus_tag="VYN69_000509"
     CDS             11503..13320
                     /locus_tag="VYN69_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001220923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEV1532827.1"
                     /translation="MRIKWFSLIRIIGLLLVLLYHFFQTIFPGGFFGVDVFFTFSGFL
                     ITALLIEEFSKNNEIDLIGFFRRRFYRIVPPVVLMVLVTMPFTFLVRQDYVAGIGGQI
                     AGVLGFMTNFYELLTGGSYESQFIPHLFVHNWSLAVEVHYYILWGLAVWFLSKQAKSN
                     GQLKGMVFLLSAAAFLISFFSMFIGSFLVTSYSSVYFSSLTHVYPFFLGSMLATIVGV
                     RQTTSLVKQLDKIWDLRKTLVVFGGGFGFLVLLTFFVKFTYLFAYLIGFLLASLAALA
                     MILAARVLHEKTHHIQEPKIISFLADTSYAVYLFHWPFYIIFSQLTSNLLAVLLTLIC
                     SYGFASLSFYVLEPWIAGKNTPILQTLRPLPYIHAILAAGTGILTFIVCMVTLLAPQV
                     GAFETDLTVNGLKQAATNIGQTKVMAERADANSLGIADGTMLIGDSVALRANTALQTV
                     LPGAQINAQVSVTTKTANEIMLNNSQNKFLPKTVVIATGVNNPENYKDDWDSIVKNLP
                     KGHHMILVTPYEGDKTKETYAIVEKAAAYMRELAEKTPYITIADWNQVAKEHPEIWAG
                     TDQVHFGSESSTIEAGAKLYADTIATALQTAQDKPVKSK"
     gene            13473..14624
                     /gene="nagA"
                     /locus_tag="VYN69_000510"
     CDS             13473..14624
                     /gene="nagA"
                     /locus_tag="VYN69_000510"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEV1532828.1"
                     /translation="MPNYIKADQFFYPHGVRRGGYLELVDGKFGKHVEQIPEGAEVID
                     YTGYSIAPGLVDTHIHGYAGVDVMDNNIEGTLHTMSEGLLSTGVTSFLPTTLTATYEQ
                     LLAVTENLGNHYKEATGAKIRGIYYEGPYFTETFKGAQNPTYMRDPGVEEFHSWQKAA
                     NGLLNKIALAPERDGVEDFVRTVTGEGVTVALGHSNATFDEAKKAVDAGASVWVHAYN
                     GMRGLTHRELGMVGAMYQLPHTYAELICDGHHVDPKACEILIKQKGTENIALITDCMT
                     AGGLEDGDYMLGEFPVVVANGTARLKSTGNLAGSILKLKDGLKNVVEWGIANPHEAVM
                     MASFNPAKSVHIDDVCGQIREGYDADFIVLDKDLELVATYLDGVKRYQA"
     gene            14787..15845
                     /locus_tag="VYN69_000511"
     CDS             14787..15845
                     /locus_tag="VYN69_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1532829.1"
                     /translation="MKAYTYVKPGLASFVDVDKPVIRKPTDAIVRIVKTTICGTDLHI
                     IKGDVPTCQSGTILGHEGIGIVEEVGEGVSNFKKGDKVLISCVCACGKCYYCKKGIYA
                     HCEDEGGWIFGHLIDGMQAEYLRVPHADNTLYHTPEDLSDEALVMLSDILPTGYEIGV
                     LKGKVEPGCSVAIIGSGPVGLAALLTAQFYSPAKLIMVDLDDNRLETALSFGATHKVN
                     SSDPEKAIKEIYDLTDGRGVDVAIEAVGIPATFDFCQKIIGVDGTVANCGVHGKPVEF
                     DLDKLWIRNINVTTGLVSTNTTPQLLKALESHKIEPEKLVTHYFKLSEIEKAYEVFSK
                     AADHHAIKVIIENDISEA"
     gene            15996..16361
                     /locus_tag="VYN69_000512"
     CDS             15996..16361
                     /locus_tag="VYN69_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEV1532830.1"
                     /translation="MYRVIEMYGDFEPWWFLEGWEEDIVASRKFDQYYDALKYYKTCW
                     FRLEQESPLYKSRSDLMTIFWDPEDQRWCDECDEYLQQYHSLALLQDEQVIPDEKLRS
                     GYEKQTSQERNRSCRMKLK"
     gene            16437..17378
                     /locus_tag="VYN69_000513"
     CDS             16437..17378
                     /locus_tag="VYN69_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000249584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HEV1532831.1"
                     /translation="MVQEIAQEIIRSAREKGTQDIYFVPKLDAYELHMRVGDERCKIG
                     SYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYAYDQKMASLRLSTVGDYRGHESL
                     VIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
                     VMSIEDPVEIKQDDMLQLQLNEAIGLTYGNLIKLSLRHRPDLLIIGEIRDSETARAVV
                     RASLTGATVFSTIHAKSIRGVYERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFAS
                     RDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS"
     gene            17326..18342
                     /locus_tag="VYN69_000514"
     CDS             17326..18342
                     /locus_tag="VYN69_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000355375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HEV1532832.1"
                     /translation="MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLD
                     RSALLDKQCVTQMRVGLSQGKSFSEMMKSLGCSSAIVTQLSLAEVHGNLHLSLGKIEE
                     YLDNLAKVKKKLIEVATYPLILLFFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
                     LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYVREWGNMISQ
                     GMELTQIFQMMQEQGSQLFKEIGQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGE
                     VKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIVLLYAAMLMPMYQNM
                     EVNF"
     gene            18344..18670
                     /locus_tag="VYN69_000515"
     CDS             18344..18670
                     /locus_tag="VYN69_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEV1532833.1"
                     /translation="MKKMMTFLKKAKVKAFTLVEMLVVLLIISVLFLLFVPNLAKQKE
                     AVNDKGKAAVVKVVESQAELYSLEKNEDASLSKLKEDGRITEEQAKAYKEYNDKNVGA
                     NRKVYD"
     gene            18663..19067
                     /locus_tag="VYN69_000516"
     CDS             18663..19067
                     /locus_tag="VYN69_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEV1532834.1"
                     /translation="MIKAFTMLESLLVLGFVSILALGLSGSVQSTFAAVEEQIFFMEF
                     EELYRETQKRSVASQQKTSLNLDGQTLSNGSQKLPVPKGIQAPSGQSITFDRAGGNSS
                     LAKVEFQTSKGAIRYQLYLGNGKIKRIKETKN"
     gene            19072..19332
                     /locus_tag="VYN69_000517"
     CDS             19072..19332
                     /locus_tag="VYN69_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000601743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532835.1"
                     /translation="MILLEAVVALAIFASIATLLLGQIQKNRQEEAKILQKEEVLRVA
                     KMALQTGQNQVNINGVEIQVFSSEKGLEVYHGSEQLLAIKEP"
     gene            19295..19756
                     /locus_tag="VYN69_000518"
     CDS             19295..19756
                     /locus_tag="VYN69_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000250539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1532836.1"
                     /translation="MVQNSCWQSKSHKVKAFTLLESLLALLVISGGLLLFQAMSQLLI
                     SEVRYQQQSEQKEWLLFVDQLEAELDRSQFEKVESNRLYMKQDGKDIAIGKSKSDDFR
                     KTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES"
     gene            19734..20147
                     /locus_tag="VYN69_000519"
     CDS             19734..20147
                     /locus_tag="VYN69_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGF"
                     /protein_id="HEV1532837.1"
                     /translation="MWKKKKVKAGVLLYAVTMAAIFSLLLQFYLNRQVAHYQDYALNK
                     EKLVAFAMAKRTKDKAEQESGEQVFNLGQVSYQNKKTSLVTTVRTDKSQYEFLFPSVK
                     IKEEKRDKKEEVATDSSEKAEKKKSEEKPEKKENS"
     gene            20284..20871
                     /locus_tag="VYN69_000520"
                     /pseudo
     CDS             20284..20871
                     /locus_tag="VYN69_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679792.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
     gene            20932..21885
                     /locus_tag="VYN69_000521"
     CDS             20932..21885
                     /locus_tag="VYN69_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1532838.1"
                     /translation="MDFEKIEQAYIYLLENVQVIQSDLATNFYDALVEQNSIYLDGET
                     ELNQVKDNNQALKRLALRKEEWLKTYQFLLMKAGQTEPLQANHQFTPDAIALLLVFIV
                     EELFTEEEITILEMGSGMGILGATFLTSLDKKVDYLGMEVDDLLIDLAASMADVIGLQ
                     AGFVQGDAVRPQMLKESDVVISDLPVGYYPDDAVASRHQVASSQEHTYAHHLLMEQGF
                     KYLKSDGYAIFLAPSDLLTSPQSDLLKVWLKEEASLVAMISLPENLFANAKQSKTIFI
                     LQKKSEIAVEPFVYPLASLQDASVLMKFKENFQKWTQGTEI"
     gene            21936..23126
                     /locus_tag="VYN69_000522"
     CDS             21936..23126
                     /locus_tag="VYN69_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEV1532839.1"
                     /translation="MTKTIAINAGSSSLKWQLYLMPEEKVLAKGLIERIGLKDSISTV
                     KFDGRSEQQILDIENHIQAVKILLDDLIRFDIIKAYDEITGVGHRVVAGGEYFKESTV
                     VEGDVLEKVEELSLLAPLHNPANAAGVRAFKELLPDITSVVVFDTSFHTSMPEKAYRY
                     PLPTKYYTENKVRKYGAHGTSHQFVAGEAAKLLGRPLEDLKLITCHIGNGGSITAVKA
                     GKSVDTSMGFTPLGGIMMGTRTGDIDPAIIPYLMQYTEDFNTPEDISRVLNRESGLLG
                     VSANSSDMRDIEAAVAEGNHEASLAYEMYVDRIQKYIGQYLAVLNGADAIVFTAGVGE
                     NAENFRRDVISGISWFGCDVDDEKNVFGVTGDISTEAAKIRVLVIPTDEELVIARDVE
                     RLKK"
     gene            23275..23646
                     /gene="rnpA"
                     /locus_tag="VYN69_000523"
     CDS             23275..23646
                     /gene="rnpA"
                     /locus_tag="VYN69_000523"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEV1532840.1"
                     /translation="MKKNFRVKREKDFKAIFKEGTSFANRKFVVYQLENQKNHFRVGL
                     SVSKKLGNAVTRNQIKRRIRHIIQNAKGSLVEDVDFVVIARKGVETLGYAEMEKNLLH
                     VLKLSKIYREGNGSEKETKVD"
     gene            23621..24445
                     /locus_tag="VYN69_000524"
     CDS             23621..24445
                     /locus_tag="VYN69_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000727905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEV1532841.1"
                     /translation="MKKKLKLTSLLGLSLLIMTACATNGVTSDITAESADFWSKLVYF
                     FAEIIRFLSFDISIGVGIILFTVLIRTVLLPVFQVQMVASRKMQEAQPRIKALREQYP
                     GRDMESRTKLEQEMRKVFKEMGVRQSDSLWPILIQMPVILALFQALSRVDFLKTGHFL
                     WINLGSVDTTLVLPILAAVFTFLSTWLSNKALSERNGATTAMMYGIPVLIFIFAVYAP
                     GGVALYWTVSNAYQVLQTYFLNNPFKIIAEREAVVQAQKDLENRKRKAKKKAQKTK"
     gene            24464..25450
                     /locus_tag="VYN69_000525"
     CDS             24464..25450
                     /locus_tag="VYN69_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein jag"
                     /protein_id="HEV1532842.1"
                     /translation="MVVFTGSTVEEAIQKGLKELDIPRMKAHIKVISREKKGFLGLFG
                     KKPAQVDIEAISETTVVKANQQVVKGVPKKINDLNEPVKTVSEETVDLGHVVDAIKKI
                     EEEGQGISDEVKAEILKHERHASTILEETGHIEILNELQIEEAMREEAGADDLETEQD
                     QTENQDLKEMGLKVEQSYDIAQVATDVTAYVQAIVDDMDVEATLSNDYNRRSINLQID
                     TNEPGRIIGYHGKVLKALQLLAQNYLYNRYSKTFYVTINVNDYVEHRAEVLQTYAQKL
                     ANRVLEEGRSHKTDPMSNSERKIIHRIISRMDGVTSYSEGDEPNRYVVVDTE"
     gene            25501..26124
                     /locus_tag="VYN69_000526"
     CDS             25501..26124
                     /locus_tag="VYN69_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P"
                     /protein_id="HEV1532843.1"
                     /translation="MLNKIRDYLDFSGLQYRSPDKAGAEREKMLAFRHKGQEARKAFT
                     ELAKAFQASHPEWQLQQTSQWMNQAQCLRPHFWIYLQRDGQVTEPMMALRLYGNPSDF
                     GISLEVSFIEHKKDEQTLGKQAKVLDIPTVKGIYYLTYSNGQSQRWEANEEKRRTLRE
                     KVRSQEVRKVLVKVDVPMTENSSEEEIVEGLLKSYSKILPYYLATRK"
     gene            26255..27712
                     /locus_tag="VYN69_000527"
     CDS             26255..27712
                     /locus_tag="VYN69_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1532844.1"
                     /translation="MEVVSSVLNWFSSNILQNPAFFVGLLVLIGYALLKKPAHDVFSG
                     FVKATVGYMLLNVGAGGLVTTFRPILAALNYKFQIGAAVIDPYFGLAAANNKIAAEFP
                     DFVGTATTALLIGFGINILLVALRKITKVRTLFITGHIMVQQAATVSLMVLFLVPQLR
                     NAYGTAAIGIICGLYWAVSSNMTVEATQRLTGGGGFAIGHQQQFAIWFVDKVAGRFGK
                     KEESLDNLKLPKFLSIFHDTVVASATLMLVFFGAILLILGPDIMSNKEVITSGTLFNP
                     AKQDFFMYIIQTAFTFSVYLFVLMQGVRMFVSELTNAFQGISNKLLPGSFPAVDVAAS
                     YGFGSPNAVLSGFTFGLIGQLITIVLLIVFKNPILIITGFVPVFFDNAAIAVYADKRG
                     GWKAAVILSFISGVLQVALGALCVALLDLASYGGYHGNIDFEFPWLGFGYIFKYLGIV
                     GYVLVCLFLLVIPQLQFAKAKDKEKYYNGEVQEEA"
     gene            27735..28016
                     /locus_tag="VYN69_000528"
     CDS             27735..28016
                     /locus_tag="VYN69_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1532845.1"
                     /translation="MVKVLAACGNGMGSSMVIKMKVENALRKLNQTDFTVNSCSVGEA
                     KGLAVGYDIVIASLHLIQELEGRTNGKLIGLDNLMDDKEITEKLSQALQ"
     gene            28093..28578
                     /locus_tag="VYN69_000529"
     CDS             28093..28578
                     /locus_tag="VYN69_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1532846.1"
                     /translation="MNLKQALIDNDSIRLGLEANNWKEAVKVAVDPLIESGAILPEYY
                     DAIIESTEEYGPYYILMPGMAMPHARPEAGVQSDAFSLITLQNPVVFSDGKEVSVLLA
                     LAATSSKIHTSVAIPQIIALFELEDSIARLQACQTKEDVLAMIEESKDSPYLEGLDLE
                     S"
     gene            28595..29260
                     /locus_tag="VYN69_000530"
     CDS             28595..29260
                     /locus_tag="VYN69_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEV1532847.1"
                     /translation="MTKRIPNLQVALDHSDLQGAIKAAVSVGQEVDIIEAGTVCLLQV
                     GSELAEVLRSLFPDKIIVADTKCADAGGTVAKNNAVRGADWMTCICCATIPTMEAALK
                     AIKTERGERGEIQIELYGDWTFEQAQLWLDAGISQAIYHQSRDALLAGETWGEKDLNK
                     VKKLIDMGFRVSVTGGLDVDTLKLFEGVDIFTFIAGRGITEAADPAGAARAFKDEIKR
                     IWG"
     gene            29264..30127
                     /locus_tag="VYN69_000531"
     CDS             29264..30127
                     /locus_tag="VYN69_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000252119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEV1532848.1"
                     /translation="MVRPIGIYEKATPTHFTWLERLNFAKELGFDFVEMSIDERDERL
                     ARLDWSKEERLEVVKAIYETGVRIPSICFSGHRRYPLGSKDPVLEEKSLELMKKCIEL
                     AQDLGVRTIQLAGYDVYYEEKSPQTRQRFIKNLRKACDWAEEAQVVLAIEIMDDPFIS
                     SIEKYLAIEKEIDSPFLFVYPDIGNVSAWHNDIYSEFYLGHHAIAALHLKDTYAVTES
                     SKGQFRDVPFGQGCVKWEEAFDILKETNYNGPFLIEMWSENCETVEETRAAIQEAQAF
                     LYPLIKKAGLM"
     gene            30129..30833
                     /locus_tag="VYN69_000532"
     CDS             30129..30833
                     /locus_tag="VYN69_000532"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEV1532849.1"
                     /translation="MNQVINAMRKRVCDANQSLPKHGLVKFTWGNVSEVNRELGVIVI
                     KPSGVDYDELTPENMVVTDLDGKILEGDLRPSSDLPTHVQLYKAWSEIGSVVHTHSTE
                     AVGWAQAGRDIPFYGTTHADYFYGSIPCARSLTKDEVEVAYEKDTGLVIVEEFEHRGL
                     NPVEVPGIVVRNHGPFTWGKNPEDAVYHSVVLEEVSKMNRFTEQINSRVEPAPQYILE
                     KHYQRKHGPNAYYGQK"
     gene            31012..32685
                     /locus_tag="VYN69_000533"
     CDS             31012..32685
                     /locus_tag="VYN69_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1532850.1"
                     /translation="MVLDKASCDLLQYLMDQETSKTIMAISKDLKESRRKIYYHIDKI
                     NAALVDEALHIISIPRIGIHLTEEQRDACCKLLSEVDSYDYIMSAHERMMIMLLWIGI
                     SKERITIEKLIELTEVSRNTVLNDLNSIRYQLTLEQYQVTLQVSKSQGYHLHAHPLNK
                     IQYLQSLLYHIFMEENATFVSILEDKMKERLDDECLLSVEMNQFFKEQVPLVEQDLGK
                     KINHHEITFMLQVLPYLLLSCHNVEQYQERHQDIEKEFSLIRKRIEYQVSKKLGERLF
                     QKFEISLSGLEVSLVAVLLLSYRKDLDIHAESDDFRQLKLALEEFIWYFESQIRMEIE
                     NKDDLLRNLMIHCKALLFRKTYGIFSKNPLTKQIRSKYGELFLVTRKSAEILEGAWFI
                     RLTDDDIAYLTIHIGGFLKYTPSSQKNMKKVYLVCDEGVAVSRLLLKQCKLYFPNEQI
                     DTVFTTEQFKSVEDIAQVDVVITTNDDLDSRFPILRVNPILEAEDILKMLDYLKHNIF
                     RNKSKSFSENLSSLISSYIVDSKLASKFQEEVQTLINQEIVVQAFLEDI"
     gene            32797..33888
                     /gene="ulaG"
                     /locus_tag="VYN69_000534"
     CDS             32797..33888
                     /gene="ulaG"
                     /locus_tag="VYN69_000534"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEV1532851.1"
                     /translation="MPNVKEITRESWILATFPEWGTWLNEEIEEEVVPEGNFAMWWLG
                     NCGTWIKTPAGANVVMDLWSNRGKSTKKVKDMVRGHQMANMAGVRKLQPNLRVQPMVI
                     DPFAINELDYYLVSHFHSDHIDPYTAAAILNNPKLEHVKFIGPYHCGRIWEGWGVPKE
                     RIIVVKPGDTIELKDMKIHAVESFDRTCLVTLPVNGADETGGELAGLAVTDEEMAQKA
                     VNYIFETPGGTIYHGADSHFSNYFAKHGKDFKIDVALNNYGENPVGIQDKMTSIDLLR
                     MAENLRTKVIIPVHYDIWSNFMASTNEILELWKMRKDRLQYDFHPFIWEVGGKYTYPQ
                     DQHLVEYHHPRGFDDCFEQDSNIQFKALL"
     gene            34002..35978
                     /gene="tkt"
                     /locus_tag="VYN69_000535"
     CDS             34002..35978
                     /gene="tkt"
                     /locus_tag="VYN69_000535"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1532852.1"
                     /translation="MSNLSVNAIRFLGIDAINKANSGHPGVVMGAAPMAYSLFTKQLH
                     INPAQPNWINRDRFILSAGHGSMLLYALLHLSGFEDVSMDEIKSFRQWGSKTPGHPEF
                     GHTAGIDATTGPLGQGISTATGFAQAERFLAAKYNREGYNIFDHYTYVICGDGDLMEG
                     VSSEAASYAGLQKLDKLVVLYDSNDINLDGETKDSFTESVRDRYNAYGWHTALVENGT
                     DLEAIHAAIETAKASGKPSLIEVKTVIGYGSPNKQGTNAVHGAPLGADETASTRQALG
                     WDYEPFEIPEQVYADFKEHVADRGASAYQAWTKLVADYKEAHPELAAEVEAIIDGRDP
                     VEVTPADFPALENGFSQATRNSSQDALNVVAAKLPTFLGGSADLAHSNMTYIKTDGLQ
                     DDTNRLNRNIQFGVREFAMGTILNGMALHGGLRVYGGTFFVFSDYVKAAVRLSALQGL
                     PVTYVFTHDSIAVGEDGPTHEPVEHLAGLRAMPNLNVFRPADARETQAAWYLAVTSEK
                     TPTALVLTRQNLTVEDGTDFDKVAKGAYVVYENAADFDTILIATGSEVNLAVSAAKEL
                     ASQGEKIRVVSMPSTDVFDKQDAAYKEEILPNAVRRRVAVEMGASQNWYKYVGLDGAV
                     LGIDTFGASAPAPKVLAEYGFTVENLVKIVRNLK"
BASE COUNT        10774 a         6400 c         8225 g        10590 t
ORIGIN      
        1 tagggagttt agctcagctg ggagagcatc tgccttacaa gcagagggtc agcggttcga
       61 tcccgttaac tcccatttta gcgggtgtag tttagtggta aaactacagc cttccaagct
      121 gttgtcgcga gttcgattct cgtcacccgc tttgaacttt gttctttgta ccaagttttt
      181 gacttgggcg cgtagctcag gtggttagag cgcacgcctg ataagcgtga ggtcggtggt
      241 tcgagtccac tcgtgcccat agtgtttagt ccattactag gggattggaa tattatctgt
      301 tcactaagag gacacgggct tgttcccgta taaaactatt ttggaggatt acccaagtcc
      361 ggctgaaggg aacggtcttg aaaaccgtca ggcgtgtaaa agcgtgcgtg ggttcgaatc
      421 ccacatcctc cttttatatt aacgcgggat ggagcagctc ggtagctcgt cgggctcata
      481 acccgaaggt cgtaggttca aatcctgctc ccgcaataag gctcggtagc tcagttggta
      541 gagcaatgga ttgaagctcc atgtgtcggc ggttcgattc cgtctcgcgc catttatata
      601 ttttggaagg gtagcgaaga ggctaaacgc ggcggactgt aaatccgctc cttcgggttc
      661 gggggttcga atccctcccc ttccatttta cgggcatagt ttaaaggtag aactaaggtc
      721 tccaaaacct tcagtgtggg ttcaattcct actgcccgtg ttaatagaat tatggcgggt
      781 gtggtgaagt ggttaacaca ccagattgtg gctctggcat gcgtgggttc gatccccatc
      841 actcgcctat tttatattgg ggtatagcca agcggtaagg caagggactt tgactccctc
      901 atgcgttggt tcgaatccag ctaccccagt tactatttgc cggcgtggcg gaattggcag
      961 acgcgctgga ctcaaaatcc agtgtccgca aggacgtgcc ggttcgaccc cggccgccgg
     1021 tatagtatag tgttaggaac gttgttattc ttcgttcctt ttttatatta tttttggtat
     1081 aattatagtt attcaaattt tgtttagatt gagaaagtgt agggtagtat gtcttgttct
     1141 atcgatttat taaaacatcg gtatttgaaa aatattaaag aaaatcctga attgtttgtc
     1201 ggaattgagt tggagtatcc tgttgcaagt ttagaagggg atgctacaga tgttgaagtt
     1261 ataaaggatc tatttcatta tttagtttct actttggatc tcaccgtagc aaaggtagat
     1321 gattttggca atctgatcca gttagtagat ccgataagtc aggatgctat tttatttgaa
     1381 gtttcctata caacgattga gtttgcattt ggtaaggctg aaacgattca agaggtcgaa
     1441 aatcgtttca ataattatat gaatgtaatt cagagaaagt tagctgaatc aaatcatgct
     1501 attgttggct gtggtatcca tcccaactgg gataaaaatg agaattgtcc agtggcttat
     1561 ccacgctatc agatgttgat ggattatttg aatttgagta gaaatattat taaatcagat
     1621 ttacatcatt tccctgaata tggtactttt atctgtggga gccaggttca gctggatatt
     1681 tcaaaaacca actacttacg ggtgattaat gcttttactc aaattgaagc ggctaaggct
     1741 tatttatttg caaactctga attttcgggt gcggattggg atacgaaaat ttcaagggat
     1801 attttctggg aagaatctat gcatggtatc tatccagaga atgttggggt caatgctaga
     1861 ctccttaatg atgaagctga tttttttgac tatctaaatc attctgcgat ttttactgcg
     1921 gaacgtgatg ggcagaccta ttatttttat cctattcagg ctggggacta tttggctacg
     1981 cctgaaatcc aagcatttgc tctgaatggg gatgaggtta ttatttaccc ccaagagaag
     2041 gattttgaaa ctcatcgtag ttaccagtac caagacttaa cgactcgagg aacagttgag
     2101 tttcgtagtg tgtgtacaca gccacttgat aggacttttg cttctgcagc ttttcacttg
     2161 ggattattgg ttaatttaga caagttagaa gcttacttag aaacagcacc tttctttaaa
     2221 gtatttggtt atgattacaa gtctttaagg agacaatttt ctaagaaaaa tcttacagat
     2281 gaggaagaaa ctacgattat tgaattttcc aaagacttac tcctactagc tgaggaggga
     2341 ctagtggtga gaaataagga agaaatgacc tatttacagc ctttgagaga agaattgagc
     2401 ctataatttc tcttataaag ggagaatttt ctgaaaaatc atgatataat ggacgagact
     2461 atagataaag gatagagagt aatgacatta gtttatcaat caacgcgtga tgccaacaat
     2521 acagtaactg ccagccaagc aattttgcaa ggtttggcga cggacggcgg tttgtttaca
     2581 ccggttactt atccaaaggt agatttggac tttgacaaat tgaaagatgc ttcttaccag
     2641 gaagttgcta agctagtttt gtcagcattt ttagatgact ttacagctga ggagttggac
     2701 tactgtatca acaatgccta cgatagcaaa tttgatactc cagctattgc accattagtg
     2761 aaattagatg ggcaatacaa tttggaactt ttccatggtt caacgattgc ctttaaggat
     2821 atggccttgt ctattttgcc atactttatg acgactgctg ctaagaaaca tggtttggag
     2881 aacaagattg ttatcttgac agcgacatct ggtgacacgg ggaaagctgc tatggcgggg
     2941 tttgcggatg tgcctggtac tgagattatc gtcttttatc caaagggtgg tgtcagcaag
     3001 gtacaagagt tgcaaatgac cactcagaat ggtgacaata ctcatgttat cgctatagat
     3061 ggtaactttg acgatgcgca aaccaacgtg aagcacatgt ttaacgacgt ggctcttcgt
     3121 gaaagattag cggccaacaa gttgcaattt tcatctgcta actctatgaa tatcggtcgt
     3181 ttggtgccac agattgttta ttatgtttat gcctatgctc agttggtcaa gactggtgag
     3241 atcgtggctg gtgataagat taacttcaca gtaccaacag gaaactttgg aaatatcttg
     3301 gctgcctttt atgccaaaca aatcggtctc ccagttggta aattgatctg tgcttcaaat
     3361 gacaacaatg ttttgacaga cttcttcaag acacgtgttt acgacaagaa acgtgagttt
     3421 aaggtaacaa ccagcccatc tatggatatc ttggtatctt caaacttgga gcgtttgatt
     3481 ttccatcttt tggggaatga tgcggttaag acggctgaac tcatgaatgc cttgaataca
     3541 caaggacaat atgaactgac tgattttgat gcagagattc tggagctctt tgcagctgaa
     3601 tatgcgactg aggaagaaac agcggcagaa atcaagcgtg tttatgagtt agattcttat
     3661 attgaggatc cacatacggc ggttgcctca gcagtttata gaaaatacca agcggttact
     3721 aatgatgcga ctaagacagt gattgcttca acagctagtc cctacaagtt cccagtagtt
     3781 gcagtagaag ctgtaactgg aaaagcaggt ttaacagact ttgaagcttt ggctcaatta
     3841 catgaaatct caggcgttgc agtgccacca gcagttgatg ggcttgagac agctcctgtt
     3901 tgtcacaaga caacagtggc agctgctgac atgcaagtag cggttgaggc ttatttagga
     3961 ctttaagata gagggagtaa actcggttgg gaaaccaact gagtttcttt tcatcaggag
     4021 gagagattgt ttaagaaaaa taaagacatt tttaatattg cattgccagc tatgggtgaa
     4081 aactttttgc agatgctaat gggaatggtg gacagttatt tggttgctca tttaggattg
     4141 atagctattt caggggtttc agtagctggt aatattatca ccatttatca ggcgattttc
     4201 atcgctctgg gagctgctat ttccagtgtt atttcaaaaa gcatagggca gaaagaccag
     4261 tcgaagttgg cctatcatgt gactgaggcg ttgaagatta ccttactatt aagtttcctt
     4321 ttaggatttt tgtccatctt cgctgggaaa gagatgatag aacttttggg gacggagagg
     4381 gatgtagctg agagtggtgg actgtatcta tctttggtag gcggatcgat tgttctctta
     4441 ggtttaatga ctagtttggg agccttgatt cgtgcaacgc ataatccacg tctgcctctc
     4501 tatgttagtt ttttatccaa tgccttgaat attctttttt caagtctagc tatttttgtt
     4561 ctggatatgg ggatagctgg tgttgcttgg gggacaattc tgtctcgttt ggttggtctt
     4621 gtgattttgt ggtcacaatt aaaactgcct tatgggaagc caacttttgg tttagataag
     4681 gaactgttga ccttggcttt accagcagct ggagagcgac ttatgatgag ggctggagat
     4741 gtagtgatca ttgccttggt cgtttctttt gggacggagg cagttgctgg gaatgcaatc
     4801 ggagaagtct tgacccagtt taactatatg cctgcctttg gcgtcgctac ggcaacggtc
     4861 atgctgttgg cccgagcagt tggagaggat gattggaaaa gagttgctag tttgagtaaa
     4921 caaacctttt ggctttctct gttcctcatg ttgcccctgt cctttagtat atatgtcttg
     4981 ggtgtaccat taactcatct ctatacgact gattctctag cggtggaggc tagtgttcta
     5041 gtaacactgt tttcactact tggaacccct atgacgacag gaacagtcat ctatacggca
     5101 gtctggcagg gattaggaaa tgcacgcctc cctttttatg cgacaagtat aggcatgtgg
     5161 tgtatccgca ttggaacagg atatctgatg gggattgtgc ttggttgggg cttacctggt
     5221 atttgggcag ggtctctctt ggataatggt tttcgctggt tatttctacg ctatcgttac
     5281 cagcgctata tgagcttgaa aggataggaa atgcaaaaaa cagcttttat ttgggattta
     5341 gacgggactt tattggactc ttacgaagcg attttatcag ggattgagga gacttttgct
     5401 cagttttcta ttccttatga taaggagaag gtgagagagt ttatcttcaa gtattcggtg
     5461 caaaatttgc ttgtgcgggt ggcagaagat agaaatctgg atgttgaggt gctaaatcag
     5521 gtgcgtgccc agagtctggc tgagaagaat gctcaggtag ttttgatgcc aggtgcgcgt
     5581 gaggtgctag cttgggcaga cgaatcagga attcagcagt ttatatatac tcataagggg
     5641 aacaacgctt ttaccattct caaggacttg ggggtggaat cctattttac agagatttta
     5701 accagtcaga gtggctttgt gcggaagcca agtccagaag cggctaccta tctgctagat
     5761 aagtatcagt tgaattctga taatacttat tatatagggg atcggactct ggatgtggaa
     5821 tttgcccaga atagtgggat tcaaagtatc aactttttag agtctactta tgaagggaat
     5881 cacaggattc aagcgttagc agatatttcc cgtatttttg agactaagtg ataaaaagat
     5941 tgtgtcagtt ttgtgacaga gacctaacaa actatttcaa gtaacctagt ttgttacaag
     6001 gaatagacag ttctgttaaa taggcccgag agggcttttt ttctacattt tttgtgttat
     6061 gatagacagg tactcatttg aaaggaattt gaaagaatga agaaaagaat gttattagcg
     6121 tcaacagtag ccttgtcatt tgccccagta ttggcaactc aagcagaaga agttctttgg
     6181 actgcacgta gtgttgagca aatccaaaac gatttgacta aaacggacaa caaaacaagt
     6241 tataccgtac agtatggtga tactttgagc accattgcag aagccttggg tgtagatgtc
     6301 acagtgcttg cgaatctgaa caaaatcact aatatggact tgattttccc agaaactgtt
     6361 ttgacaacga ctgtcaatga agcagaagaa gtaacagaag ttgaaatcca aacacctcaa
     6421 gcagactcta gtgaagaagt gacaactgcg acagcagatt tgaccactaa tcaagtgacc
     6481 gttgatgatc aaactgttca ggttgcagac ctttctcaac cgattgcaga agctccaaaa
     6541 gaagtagcat caagttcaga agttacaaag acagtgattg cttctgaaga agtggcacca
     6601 tctacgggca cttctgtccc agaggagcaa acggccgaaa caagcagtgc agttgcagaa
     6661 gaagctcctc aggaaacgac tccagctgag aagcaggaaa cacaagtgag ctctcaaacc
     6721 gagtcagcag tggaagcaac tacaatgcca gtagaagaaa aagcaaccga aacaactgca
     6781 acaagttcag aagcaaaaga agtagcatca tcaaatggag ctacagcagc agtttctact
     6841 tatcaaccag aagagacgaa aacaatttca acaacttacg aggctccagc tgcgcccgat
     6901 tatgctggac tggcagtagc gaaatctgaa aatgcaggtc ttcaaccaca aacagctgcc
     6961 tttaaagaag aaattgctaa cttgtttggc attacatcct ttagtggtta tcgtccagga
     7021 gacagtggag atcacggaaa aggtttggct atcgacttta tggtaccaga aagttcagaa
     7081 ttaggggata agattgcgga atatgctatt caaaatatgg ccagccgtgg cattagttac
     7141 atcatctgga aacaacgttt ctatgctcca ttcgatagca aatatgggcc agctaacact
     7201 tggaacccaa tgccagaccg tggtagtgtg acagaaaatc actatgatca cgttcacgtt
     7261 tcaatgaatg gataaacccg acttgataac atcattttga cgaatgagat ctagctttcg
     7321 tgatggaaag cgattctcgt tcgttttttc tttgtcatac tcttcgaaaa tctcttcaaa
     7381 ccacgtcagt tttatctgaa acctcaaagc tgtgctttga gcaacctgcg actagcttcc
     7441 tagtttgctt tttgattttc attgagtatc aatttgaatg gaaaatggaa agttatcatc
     7501 ttgtaatgag ttaagcaaca ttcttgcaat ctattttata gtagatggaa actagaatag
     7561 tacacaactg cttctaaaac attgttagaa atcgatttga ctgtcctgat cgatttgtcc
     7621 tgctcttatt tcatttcact atactttata ttataattga ctggtcaact attgataaat
     7681 caataaaaag agaggggaag aaatgctaga gattcaagat ttactgtatc aactccgctt
     7741 gtctgagcaa gcgagtacgc aattgtttga aaaaaggctt ggattagttt gacacggtat
     7801 cagattttac tgtttttgct ggagcattct ctttgtaacc aaatggcggt tcaggagcgt
     7861 ttgaaaattg atcaggctgc tttgacacgg catttcaaga ttttggaaac ggaaggtttg
     7921 gtggagcgtc atcgtaatcc tgaaaatcag cgggaagtgt tggtagaggc tgcgaagtat
     7981 gccaaggagc agttagtggt gaatccccct ctgcaacata tcaaggttaa ggaagagatg
     8041 gaaaatatct taacagagtt tgagagaaca gaactcagcc gtttattaaa taaattggtt
     8101 ttgggtattg aaaatataga aatttaagga gaaatagatg tcaattattt taacaacgat
     8161 cgttgctttg gagcattttt acatttttta tttggaaagt attgccacgc aatcagatgc
     8221 gactagtcgt gtatttaata tggaaaagga agaattggct catccgtcag taagttcatt
     8281 gttcaaaaat caaggaattt ataaggatct gctaggagtc tttctcttgt atggcattta
     8341 tttctcacag aatttagaaa ttgtgactat ttttgtctta tttgtgattg gtgctgcgac
     8401 ttacggctct ttaacagcgg ataaaaaaat tattttgaaa caaggtggat cagctatttt
     8461 ggccttgatt agtattttac tctttaaata cacttgaagg tcgattctaa tctcgctaat
     8521 cctttttaat ccagaataag ggaaatatgt tatacttgtt tttaagaaaa aagtctcatt
     8581 gaattggttt tgaggagtta gaaatgaaag tattagtgac aggttttgag ccctttggag
     8641 gggaaaaggg caatccagct ttggaggcca ttaaaggttt accagctgaa atccatggtg
     8701 ctgaggtccg ttggctagag gtgccgacag tttttcacaa atctgctcaa gtattggaag
     8761 aagagatgaa tcgttatcaa cctgactttg tcctttgtat tgggcaagct ggtggaagaa
     8821 ctagtttgac acctgaacga gtggccatta atcaagacga tgcacgtact tctgataacg
     8881 aagataatca accgattgac cgtcccattc gcccagatgg tgcttcggcc tactttagta
     8941 gtttgccgat taaagcgatg gttcaagcta taaaaaaaga gggcttaccg gcctctgttt
     9001 ccaatacggc agggactttt gtctgcagcc atttgatgta tcaggctctc tatttggtag
     9061 aaaagaaatt tccatatgtt aaggcaggtt ttatgcatat tccttatatg atggaacagg
     9121 tggtgaacag acagactact ccagctatga gtttagtgga tattcggcga gggatagaag
     9181 cagcaatcgg cgctataata gaacatggag atcaggaact caagttggta ggcggagaaa
     9241 ctcattgata gaaaaaagct tgaggggaaa aaccttcaag cttttggacg ttttcgggcc
     9301 aatactgctc ggtaaaacat aattttagtg cattggatat aaggtaggag tgaaaaacta
     9361 gcaatgccaa aggtaatcca attgaggaag taccaaggaa gaagctgtaa atctaggaca
     9421 aagtgctgga acttgtagcc cttcataaag gaacggctag tttttaggat tcgtcttggt
     9481 gagacctgtc ctaggtctag actataacag agaagaaatt ccacctgtga ataggcataa
     9541 tactgtggaa tatagaggat atttcctaca atgatcaaga tgagacttgc aagaaagtag
     9601 agtccaaaga ccatgaggaa acgctcggtt tcaactgatg agagatctag atttggaaac
     9661 tcaggatgta gggtgacgaa ttttttggct aaaaagctac tataaaagag gaggtaaatc
     9721 ccaagtaaat tagggatact ccataaaaag agatagaaac gtttgagaag tagggtcaaa
     9781 aaggtttgag aaaagcgctc ctcatcaaag agagctaggc tgttttttac agatggctcc
     9841 gttttagaat ctttcatgag tgtcagtgtt gcatagacgg aactggtcaa aagaatagtc
     9901 ccgataaagg agactagtag aggaaagagg taggtttgaa gtatttggcc aagtatgctg
     9961 aaaaatggct gttctaaaac agtcccgtgg atccgagata agggattaag aaaactagat
    10021 aagatgacca gcatactggg aaggatatag aggagaaaga gacggggggt gtcagcctga
    10081 aaatgttttg actcctgacg aattgttttt aaatcaattt ttggatagtt cattctctta
    10141 ttataccata gttcttatac atagttcgtg acagttccta ctttttttga taaaatcata
    10201 cagtgtgtcc ttgggcacac tgtatgaact gggactgtct ttcccagctt cggaggtaaa
    10261 aaatgtcaga ttcaccaatc aaatatcgtt tgattaagaa agaaaaacac acaggagctc
    10321 gtctaggaga aatcatcact ccccacggta cctttccgac acctatgttt atgccagttg
    10381 ggacacaagc cactgtcaaa actcagtcac ctgaagaatt gaaggagatg ggttcgggaa
    10441 ttatcctatc aaacacctat catctctggc ttcgccctgg agatgaactc attgcacgcg
    10501 ctggtggtct ccacaagttc atgaattggg accagcctat cttgacagat agtggtggtt
    10561 ttcaggttta ttctttagca gatagccgta atatcacaga agaaggagta acctttaaaa
    10621 atcatctaaa tggttctaag atgttcctat ccccagaaaa agccatctct attcagaata
    10681 atctgggttc agacatcatg atgtcctttg atgaatgtcc tcagttttat caaccttatg
    10741 actacgttaa gaaatcgatc gagcgtacca gccgttgggc tgagcgtggt ttgaaggctc
    10801 accgtcgtcc acatgaccaa ggtttgtttg gaattgtgca aggtgcagga tttgaagacc
    10861 ttcgccgcca atcagctcat gatcttgtca gcatggattt ctcaggctac tctatcggtg
    10921 gtttggcagt gggagaaacc catgaagaga tgaatgcggt cttggacttt acaactcaac
    10981 tgctacctga aaataaacct cgttatctga tgggtgtggg agcgccagat agcttgatcg
    11041 atggggtcat tcgtggggtg gatatgtttg actgtgtctt accgactcga attgctcgta
    11101 acgggacttg tatgaccagt caaggacgtt tggttgtgaa aaatgcccag tttgctgagg
    11161 actttacgcc actggatcct gagtgtgact gctacacatg taataactat acacgcgctt
    11221 accttcgtca cctgctcaag gctgatgaaa cctttggtat ccgcttgact agctaccaca
    11281 atctttactt cttgcttaac ctgatgaagc aagtgcgaca agccatcatg gatgacaatc
    11341 tcttggaatt ccgtgagtat tttgtggaaa aatatggcta taataagtca ggacgtaatt
    11401 tctaaaatgg aattgatata aaaaaatcct aagttttctc ttaggatttt tcttcttttt
    11461 ttgatagaat aaagtgtaca atgaaaggaa gaataaactc gtatgcgcat taaatggttt
    11521 tccttgatta ggattatagg tttacttttg gtactcttgt atcacttctt tcagacgatc
    11581 tttcctggag gattcttcgg ggtagatgtc tttttcacat tttcaggttt cctgattaca
    11641 gctctactca ttgaagaatt ttctaagaac aatgagattg atttgatagg attttttaga
    11701 agacgctttt atcggattgt gccacctgtg gttttgatgg tcttggtgac catgcctttt
    11761 actttcttgg ttcgccaaga ctatgttgct ggaattggtg gccagattgc gggcgtctta
    11821 ggctttatga ccaacttcta tgaactccta acaggtggga gttatgaatc tcagttcatt
    11881 cctcatttgt ttgttcataa ttggagtttg gcagttgagg ttcactacta tattctttgg
    11941 ggattggcag tttggttctt atccaaacaa gctaaatcaa atggtcagtt gaaggggatg
    12001 gtctttctct tatctgctgc tgccttcttg atcagtttct tctccatgtt tattggtagt
    12061 tttctagtga cctcttattc ctctgtttat ttctccagtt taactcatgt ctatccattc
    12121 tttttgggaa gtatgttagc aactattgta ggcgttcgtc agacgacttc cctcgtcaag
    12181 cagttggata aaatctggga tttacgaaag actttggtag tttttggagg aggctttggt
    12241 ttcctagttc ttttgacttt ctttgtcaaa ttcacttatc tttttgccta tcttatcggc
    12301 ttcttgcttg ccagtcttgc agctcttgcc atgattctgg cggcgcgtgt cttacatgaa
    12361 aagacacatc atatacagga gccgaagatt atcagctttt tagcggatac tagctatgcg
    12421 gtttatcttt tccattggcc tttctatatc attttctcac agttgacatc aaatcttctt
    12481 gctgtattac tgactctgat ttgttcttat ggctttgcca gtctgtcatt ctatgtattg
    12541 gaaccttgga ttgcaggcaa gaacacacct attttacaaa ctcttcgtcc cctgccttat
    12601 attcacgcaa ttcttgcagc aggtacagga atcttgacct tcattgtctg catggtgacc
    12661 ttgttggcac cacaagtggg agcgtttgag acagacttga ctgtcaatgg cttgaagcaa
    12721 gctgcaacaa atattggcca gaccaaggtg atggcagaac gggcagatgc aaacagtttg
    12781 ggaattgctg atggcactat gttaattggt gactcagtgg ctttaagggc aaatacagca
    12841 ctacagacag ttcttcctgg agcacagatt aacgcgcagg tcagcgtaac aaccaagacc
    12901 gcaaatgaaa tcatgctaaa taatagccag aataaatttt tacctaagac ggtggtcatt
    12961 gcgactgggg taaataatcc tgagaattac aaggatgact gggacagtat cgtgaaaaat
    13021 cttcctaagg gacaccatat gattttggtg actccttatg agggagataa gacaaaagag
    13081 acctatgcca tcgttgagaa ggctgctgcc tatatgagag aattggcaga gaagacacct
    13141 tacattacga tagcagattg gaatcaagtt gcgaaagagc atccagaaat ttgggcagga
    13201 acagaccagg ttcatttcgg gagtgagagt agcactatcg aagcaggagc aaaattgtat
    13261 gcagatacga ttgccacagc tttgcagaca gctcaagaca agccggttaa atcaaaataa
    13321 cttgtattaa aaaagagaag agttggagaa atccagctct tttaaaatat ctccaaaaaa
    13381 taggtctata ccatttacaa ataaaaaaga aaggtttata atgtaattga cataataaat
    13441 tgtagaatca atcttttaag gaggttaaca ttatgcctaa ctatattaaa gcggatcagt
    13501 ttttctaccc acacggagtt cgtcgaggtg gttacttgga acttgtggac ggcaagtttg
    13561 ggaaacatgt agagcagatt cctgaagggg ctgaggtgat tgactataca ggttatagca
    13621 ttgccccagg tcttgtggat actcatattc atggatatgc aggtgtagat gtgatggaca
    13681 acaacattga aggtacattg catactatga gtgaaggact tcttagtacc ggtgttacca
    13741 gtttcttacc cacaacttta acagccactt atgagcaatt gcttgcagtc actgaaaatc
    13801 ttggaaacca ttataaagaa gcaacaggtg ctaagattcg tgggatttat tatgaaggtc
    13861 catatttcac agaaactttt aagggggcac aaaatccaac ttatatgaga gacccgggtg
    13921 ttgaggagtt tcattcttgg caaaaagcgg caaatggctt gcttaataaa attgcccttg
    13981 caccagaacg tgatggggtg gaagactttg ttcgtacagt tacgggcgaa ggtgtgacgg
    14041 ttgctcttgg acattcaaac gcgacttttg atgaagccaa aaaagcagtc gatgctggag
    14101 cgagtgtttg ggtgcatgcc tacaatggaa tgcgtgggtt gactcaccgt gaattgggta
    14161 tggttggagc catgtaccaa ttgccacata cctatgcaga gttgatctgt gatggtcacc
    14221 acgtagatcc aaaggcttgc gaaattctta tcaaacaaaa aggaacagaa aacatcgctc
    14281 ttatcacaga ctgtatgaca gctgggggat tggaagacgg agattatatg ttgggagaat
    14341 tcccagttgt cgttgcaaat ggaactgcac gcctcaaatc gacaggtaac ttggcaggtt
    14401 ctatcctcaa actcaaagat ggtttgaaga atgtggtcga atggggaatt gcgaatccgc
    14461 atgaagcagt catgatggcc agcttcaacc cagctaaatc cgttcacatc gatgacgtct
    14521 gtggccaaat ccgtgaaggc tacgacgctg acttcatcgt attagataaa gatttggaat
    14581 tggtagcaac ctacctagat ggcgtgaaac gttatcaagc ataagagaga aagcagaagt
    14641 tagagactaa cttctgcttt tttatgcagg gtactttact ggtaaataaa aagttaaaaa
    14701 aatcacaaaa aaagcttgaa gaaacaaaga aaaaggctta caattgaact ataaatagta
    14761 caaataaaaa aacggaggag tgctttatga aagcctatac ttatgttaaa ccaggacttg
    14821 cttcttttgt tgatgtagac aaaccagtta ttcgcaagcc aacagacgct attgtgcgta
    14881 ttgtaaaaac cactatttgt ggaacagacc tccatattat caaaggggat gttcctactt
    14941 gccaaagtgg taccattctt ggccacgaag ggattgggat tgttgaagaa gttggggaag
    15001 gagtttccaa cttcaaaaaa ggtgacaagg tcttgatttc ttgcgtctgt gcctgtggta
    15061 aatgctacta ctgtaaaaaa ggaatttatg cccactgtga agacgaaggg ggctggattt
    15121 tcggtcactt gattgatggt atgcaggctg aatatctacg tgtccctcat gcagataata
    15181 ctctttacca tactccagaa gacttgtcag atgaagcttt ggttatgctg tcagacattc
    15241 tgcctactgg atatgaaatt ggtgtcttaa aagggaaagt agaacctggt tgcagcgtag
    15301 ccattattgg ttcaggtcca gttggattgg ctgctctttt aacagcccaa ttctattcac
    15361 cagctaaatt gattatggta gacctagacg ataaccgctt ggaaactgcc ctatcattcg
    15421 gtgcgactca taaggttaat tcttcagacc ctgaaaaagc cattaaagaa atttatgatt
    15481 tgacagatgg tcgtggtgtg gatgtcgcta tcgaagctgt tggtattcct gcaacatttg
    15541 atttctgtca aaagattatc ggtgtagacg gaacggttgc caactgtggt gtgcatggta
    15601 aaccagttga attcgattta gataaacttt ggattcgcaa catcaatgta acaactggtt
    15661 tggtatctac aaatacgact ccacaattgt tgaaagcact tgaaagtcat aagattgaac
    15721 cagaaaaatt ggtaactcac tatttcaaac tcagtgaaat tgaaaaagcc tacgaagtct
    15781 tcagtaaggc agcagaccac catgccatta aggtcattat cgaaaacgat atctcagaag
    15841 cctaagtagt aaaaatattt ttgtacataa gtaaatagaa attcagtcat ccatcagatg
    15901 gctggatttt ttatcaaaaa attaagaaat gagcatattt ctttccttgt ctggcggaat
    15961 tggttataat atacggtaca aaggaatgaa tgaatatgta tcgtgttata gaaatgtacg
    16021 gagattttga accgtggtgg ttcttagaag gttgggaaga agatattgta gcaagtagaa
    16081 aatttgatca gtattatgat gctctcaaat actacaaaac ttgctggttt agattggaac
    16141 aagaatcccc tctttataaa agtagaagcg atttgatgac cattttttgg gacccggaag
    16201 accaacgctg gtgtgatgaa tgtgatgagt atttacaaca ataccattct ttggctcttt
    16261 tgcaggatga gcaggttatc ccagacgaaa aactacgctc aggctatgaa aaacaaacca
    16321 gtcaggaaag gaatcgttct tgccgtatga aattaaaata gagaaaagta acttttttgg
    16381 agttgctttt tttatttttc taacgctttg cgaatagtat aggtgaggag gtaagtatgg
    16441 ttcaagaaat tgcacaagaa atcattcgtt cagctcggga aaaagggacg caggatattt
    16501 attttgtccc taagttagac gcctatgagc ttcatatgag ggtaggagac gagcgctgta
    16561 aaattggtag ctatgatttt gaaaagtttg cagccgttat cagtcacttt aagtttgtgg
    16621 cgggtatgaa tgtgggagaa aaaagacgta gtcaactggg ttcctgtgat tatgcctatg
    16681 accagaagat ggcgtctcta cgtttatcta ctgtaggcga ttatcggggg catgagagtt
    16741 tggttatccg tttgttgcac gatgaggaac aggacttgca tttttggttt caggatattg
    16801 aagaattagg caagcagtac aggcaacggg gactctatct ttttgctggt ccggttggga
    16861 gtggtaagac gaccttgatg catgaattgt ccaagtcact ctttaaagga cagcaagtta
    16921 tgtccatcga agatcctgtc gaaatcaagc aggacgacat gcttcagttg cagttgaacg
    16981 aagcaatcgg cctaacctat ggaaatctaa tcaaactttc cttgcgtcat cgaccagatc
    17041 tcttgattat cggagaaatt cgtgacagcg agacggcgcg tgcagtggtc agagctagtt
    17101 tgacaggtgc gacagtcttt tcaaccattc acgccaagag tatccgaggt gtttatgagc
    17161 gtctgctgga gttgggtgtg agtgaagaag aattggcagt tgttctgcaa ggagtctgct
    17221 accagagatt aatcggggga ggaggaatcg ttgactttgc aagcagagat tatcaagaac
    17281 accaagcagc caagtggaat gagcaaattg accagcttct taaagatgga catatcacaa
    17341 gtcttcaggc tgagacggaa aaaattagct acagctaagc aaaaaaatat catcacccta
    17401 tttaacaatc tcttttctag cggttttcat ctggtggaga ctatctcctt tttagatagg
    17461 agtgctttgt tggacaagca gtgtgtgacc cagatgcgtg tgggcttgtc tcaggggaaa
    17521 tcattctcag aaatgatgaa aagtttggga tgttcaagtg ctattgtcac tcagttatcc
    17581 ctagctgaag tccatggtaa tctccacctg agtttgggaa agatagaaga gtatctagac
    17641 aatctggcta aggtcaagaa aaaattaatt gaagtagcga cctatccttt gattttgctg
    17701 ttttttcttc tcttaattat gctggggcta cggaattacc tgctcccaca actggatagt
    17761 agcaatattg ccacccgaat tatcggtaat ctgccacaaa tttttctagg catggtaggg
    17821 cttgtttccg tgcttgccct tttagcacta actttttata aaagaagttc taagatgagt
    17881 gtcttttcta tcttagcacg ccttcccttt attggaatct ttgtgcagac ctacttgaca
    17941 gcctattatg tacgtgaatg ggggaatatg atttcacagg gaatggagct gacgcagatt
    18001 tttcaaatga tgcaggaaca aggttcccag ctctttaaag aaatcggtca agatctggct
    18061 caaaccctga aaaatggccg tgaattttct cagacgatag gaacctatcc tttctttagg
    18121 aaggaattga gtctcatcat agagtatggg gaagttaagt ccaagctggg tagtgagttg
    18181 gaaatctatg ctgaaaaaac ttgggaagcc ttttttaccc gagtcaaccg caccatgaat
    18241 ttggtgcagc cactggtttt tatctttgtg gcactgatta tcgttttact ttatgcggca
    18301 atgctcatgc ccatgtatca aaatatggag gtaaattttt aaaatgaaaa aaatgatgac
    18361 attcttgaaa aaagctaagg ttaaagcttt tacattggtg gaaatgttgg tggtcttgct
    18421 gattatcagc gtgcttttct tgctctttgt acctaatctg gccaagcaaa aagaagcagt
    18481 caatgacaaa ggaaaagcag ctgttgttaa ggtggtggaa agccaggcag aactttatag
    18541 cttggaaaag aatgaagatg ctagcctaag caagttaaaa gaagatggac gcatcacgga
    18601 agaacaggct aaagcttata aagaatacaa tgataaaaat gtaggagcaa atcgtaaagt
    18661 ctatgattaa ggcctttacc atgctggaaa gtctcttggt tttgggtttt gtgagtatcc
    18721 ttgccttggg cttgtccggc tctgttcagt ccacttttgc ggcggtagag gaacagattt
    18781 tctttatgga gtttgaagaa ctctatcggg aaacccaaaa acgcagtgta gccagtcagc
    18841 aaaagactag tctgaactta gatgggcaga cgcttagcaa tggcagtcaa aagttgccag
    18901 tccctaaagg aattcaggcc ccatcaggtc aaagtattac atttgaccgt gctgggggca
    18961 attcgtccct ggctaaggtt gaatttcaga ccagtaaagg agcgattcgc tatcaattat
    19021 atctaggaaa tggaaaaatt aaacgcatta aggaaacaaa aaattagggc agtgatttta
    19081 ctggaagcag tagtcgctct agctatcttt gccagcattg cgaccctcct tttgggacaa
    19141 attcaaaaaa ataggcaaga ggaagcaaaa atcttgcaaa aggaagaagt cttgagggta
    19201 gctaagatgg ccttgcagac aggtcaaaat caggtaaaca taaacggagt tgagattcag
    19261 gtgttttcta gcgaaaaggg attggaggtc taccatggtt cagaacagtt gttggcaatc
    19321 aaagagccat aaggtcaagg cttttacctt gttagaatcc ctgcttgccc tgctcgtcat
    19381 cagtggaggc ttactccttt ttcaagctat gagtcagctc ctcatttcag aagttcgcta
    19441 ccagcaacaa agcgagcaaa aggagtggct cttgtttgtg gatcaacttg aggcagaatt
    19501 agaccgttcg cagttcgaaa aagtagaaag caatcgccta tacatgaagc aagatggcaa
    19561 ggacatcgcc atcggtaagt caaagtcaga cgattttcgt aaaaccgatg ctagtggacg
    19621 ggggtatcag cctatggttt atggcctcaa atcagctcag attacagagg acaatcaact
    19681 ggttcatttt cgtttccagt ttcaaaaagg cttagaaagg gagttcatct atcgtgtgga
    19741 aaaagaagaa agttaaggca ggtgttctcc tctatgcagt caccatggca gctatcttta
    19801 gtcttttgtt gcaattttac ttgaaccgac aagtcgccca ctatcaagac tatgctttga
    19861 ataaagaaaa attggttgct tttgccatgg ctaaacgaac caaggataaa gccgagcaag
    19921 aaagtgggga acaggtcttt aatctaggtc aggtaagcta tcaaaacaag aaaacaagct
    19981 tggtgacgac tgttcgtacg gataagagtc aatatgagtt tctgtttcct tcagtcaaaa
    20041 tcaaagaaga gaaaagagat aaaaaggaag aggtagcgac tgattcaagc gaaaaagcgg
    20101 agaagaaaaa atcagaagag aagcctgaaa agaaagagaa ttcctagtca attcaactat
    20161 aatgcgttga atccagaata gtccactgta gtttctagaa aattgctgga aatggatgtt
    20221 aagctccaat tcatttgttt atatcttatt tcagtttact atactttgtg ctaaactaaa
    20281 gatatgaaac atgattttaa ccacaaagca gaaactttcg actcccctaa aaatatcttc
    20341 ctcgcaaact tggtatgtca agcagtcgag aaacagattg atattttatc agacaaagta
    20401 attttagatt tcggtggtgg cacgggtcta ttagccttgc ccctagccaa gcaggctaag
    20461 tcagttactc ttgtagacat ttctgagaaa atgttggagt aagctcgttt gaaagcggag
    20521 cagcaagcaa tcaagaatat ccagtttttg gagcaagatt taccgaaaaa tcccttggag
    20581 aaagagtttg attgccttgc tgttagtcgg gttcttcatc atatgcctga tttggatgcg
    20641 gatctctcac tgtttcatca acatttgaag gaagatggga aactcatcat tgctgatttt
    20701 accaagacag aagctaatca tcatggattt gatttagctg aactggaaaa caagctaatt
    20761 gagcatggtt tttcatctgt gcatagtcag attctctata gcgctgaaga cctgtttcaa
    20821 gggaatcact cagaattctt tttaacagta tcccaaaaat cactcgccta gtcagggagt
    20881 gatttttcta taaggatgga aaaaagaagg gaaatttgat aagataggaa tatggatttt
    20941 gaaaaaattg aacaagctta tatctattta ctagagaatg tccaagtcat ccaaagtgat
    21001 ttggcgacca acttttatga cgccttggtg gagcaaaata gcatctatct ggatggtgaa
    21061 actgagctaa accaggtcaa agacaacaat caggccctta agcgtttagc actacgcaaa
    21121 gaagaatggc tcaagaccta ccagtttctc ttgatgaagg ctgggcaaac agaacccttg
    21181 caggccaatc accagtttac gccagatgct attgccttac ttttggtatt tattgtggaa
    21241 gagttgttta cagaggagga aattactatc ctcgaaatgg gttctgggat gggaattcta
    21301 ggcgctactt tcttgacctc gcttgataaa aaggtggatt acttgggaat ggaagtggat
    21361 gatttgctga ttgatctggc agctagcatg gcagatgtaa ttggtttgca ggctggcttt
    21421 gtccaaggag atgccgttcg cccacaaatg ctcaaagaaa gcgatgtggt catcagtgac
    21481 ttgcctgttg gctattatcc tgatgatgcc gttgcgtcgc gccatcaagt tgcttctagc
    21541 caagaacata cttacgctca tcacttgctc atggaacaag ggtttaagta ccttaagtca
    21601 gatggatatg ctatttttct agctccgagt gatttgttga ccagtcctca aagtgatttg
    21661 ttgaaagtct ggttgaaaga ggaggcaagt ctggttgcta tgattagttt acctgaaaat
    21721 ctctttgcta atgctaaaca atctaagact atttttatcc tacagaagaa aagtgaaata
    21781 gcagtggagc cttttgttta tccacttgct agcttgcaag atgcaagtgt tttaatgaaa
    21841 tttaaagaaa attttcaaaa atggactcaa ggtactgaaa tataaaatag attttgttat
    21901 aatagttgaa aacgcttaaa aaggggtatc atgttatgac aaaaacaatt gcaatcaatg
    21961 caggaagttc aagtttgaaa tggcaattat acttaatgcc agaagaaaaa gtattggcga
    22021 aaggtttgat tgaacgtatc ggtttgaaag attcaatttc aactgtaaaa tttgacggcc
    22081 gttctgaaca acaaattttg gatattgaaa atcatataca agccgttaaa attttattgg
    22141 atgacttgat tcgtttcgat attatcaagg cttatgacga gattacaggt gttggacatc
    22201 gtgttgttgc tggtggagaa tatttcaaag aatcaacagt tgttgaggga gatgttttag
    22261 aaaaagttga agagttgagt ttgttggctc ctctacacaa cccggccaat gcagcaggtg
    22321 ttcgtgcctt caaggaattg ttgccagaca ttaccagtgt agttgttttt gatacttcct
    22381 tccacacaag tatgccagag aaagcttatc gctaccctct accaacaaaa tattacacag
    22441 aaaacaaggt tcgtaaatac ggtgctcatg gtacaagtca ccagtttgta gcaggagaag
    22501 ctgcaaaact cttgggacgt ccattagaag acttgaagtt aattacctgt catattggta
    22561 acggaggctc aattacagct gtgaaagccg gcaaatctgt agacacttct atggggttca
    22621 ctcctcttgg tggtattatg atgggaacgc gtacagggga tattgatcca gctatcattc
    22681 cttatttaat gcaatataca gaggatttta acacaccaga agatatcagt cgtgttctta
    22741 accgtgaatc aggtcttttg ggagtttctg ctaattctag cgatatgcgc gatatagaag
    22801 cagctgtagc agaagggaat cacgaggcta gcttggctta tgaaatgtat gttgaccgta
    22861 tccaaaaata tatcggtcag taccttgcag tgctaaatgg agcagatgcc attgttttca
    22921 cagcaggtgt cggtgaaaat gcagagaatt tccgtcgtga tgtaatctca gggatttcgt
    22981 ggtttggttg tgatgttgat gatgaaaaga atgtctttgg cgttacagga gacatctcaa
    23041 cagaggcagc taaaatccgt gtcttggtta ttccaacaga tgaagaatta gtcattgccc
    23101 gtgacgttga acgcttgaaa aaataagtga aactaaaaaa atattcaata caaggagttg
    23161 ggaaagttat ttttccagct tctttttctg atgaaattgt ccaaaacctt gctatgattg
    23221 gcttttttga aaaatatggt ataatagtag taatttaata gatggagttg agttttgaag
    23281 aaaaactttc gtgtaaaaag agagaaagat tttaaggcga ttttcaagga ggggacaagt
    23341 tttgctaatc gcaaatttgt ggtctaccaa ttagaaaacc agaaaaacca ttttcgagta
    23401 ggtctatcag ttagcaaaaa actggggaat gccgtcacta gaaatcaaat taagcgacgg
    23461 attcggcata ttatccagaa tgcaaaaggg agtctggtag aagatgtcga ctttgttgtc
    23521 attgctcgaa aaggagtcga aaccttggga tacgcagaga tggagaaaaa tctactccat
    23581 gtattaaaat tatcaaagat ttaccgggaa ggaaatggga gtgaaaaaga aactaaagtt
    23641 gactagtttg ctaggactgt ctctgttaat catgacagcc tgtgcgacta atggggtaac
    23701 tagcgatatt acagccgaat cggctgattt ttggagtaaa ttggtttact tctttgcgga
    23761 aatcattcgc tttttatcgt ttgatattag tatcggagtg gggattattc tctttacggt
    23821 cttgattcgt acagtcctct tgccagtctt tcaggtgcaa atggtggctt ctaggaaaat
    23881 gcaggaagct cagccacgca ttaaggcgct tcgagaacaa tatccaggtc gagatatgga
    23941 aagcagaacc aaactagagc aggaaatgcg taaagtattt aaagaaatgg gtgtcagaca
    24001 gtcagactct ctttggccga ttttgattca gatgccggtt attttggccc tgttccaagc
    24061 cctatcaaga gttgactttt taaagacagg tcatttctta tggattaacc ttggtagtgt
    24121 ggatacaacc cttgttcttc cgattttagc agcagtattc acctttttaa gtacttggtt
    24181 gtccaacaaa gctttgtctg agcgaaatgg cgctacgact gcgatgatgt atgggattcc
    24241 agtcttgatt tttatctttg cagtttatgc gccaggtgga gtcgccctat actggacagt
    24301 gtctaatgct tatcaagtct tgcaaaccta tttcttgaat aatccattca agattatcgc
    24361 agagcgcgag gccgtagtac aggcacaaaa agatttggaa aatagaaaaa gaaaagccaa
    24421 gaaaaaggct cagaaaacga aataaataag gaggaatctg gtaatggtag tatttacagg
    24481 ttcaactgtt gaagaagcaa tccagaaagg attgaaagaa ttagatattc caagaatgaa
    24541 ggctcatatc aaagtcattt ctagggagaa aaaaggcttt cttggtctat ttggtaaaaa
    24601 accagcccaa gtggatattg aagcgattag tgaaacgact gttgttaaag caaatcaaca
    24661 ggtagtaaaa ggcgttccga aaaaaatcaa tgatttgaac gagcctgtga agacggttag
    24721 tgaagaaacc gttgaccttg gtcatgtggt tgatgctatt aaaaaaatag aggaagaagg
    24781 tcaaggtatt tctgatgaag tcaaggctga aatcttaaaa catgaaagac atgccagcac
    24841 tatcttagaa gaaactggtc acattgagat tttaaatgaa cttcaaatcg aggaagcgat
    24901 gagggaagaa gcaggcgctg atgaccttga aactgagcaa gatcaaactg aaaatcaaga
    24961 cttgaaagag atgggcttga aggtcgagca aagttatgat attgcccagg tggctacgga
    25021 tgtgactgcc tatgttcaag cgattgtgga tgacatggat gttgaggcta cactttcaaa
    25081 tgattataac cgtcgtagca tcaatctaca aattgacacc aacgaaccag gtcgtattat
    25141 cggctaccat ggtaaagtct tgaaggcctt gcaactgttg gctcaaaatt atctttacaa
    25201 ccgctattcc aaaaccttct acgttacaat caatgtcaat gattatgtcg aacaccgtgc
    25261 agaagtcttg cagacctatg cacaaaaatt ggcgaatcgt gttttggaag aaggtcgcag
    25321 tcataaaaca gatccaatgt caaatagcga acgcaagatt atccaccgta ttatttcacg
    25381 tatggatggc gtgaccagtt actctgaagg tgatgagcca aatcgctatg ttgttgtaga
    25441 tacagaataa gtaaaatcag ggttgacctg attttttgct agttagagga ggttaaactg
    25501 atgttgaata agataagaga ctatctagac ttttctggtt tgcagtaccg tagtcctgat
    25561 aaagcgggag cagagcgaga gaagatgctg gcgttccgcc acaaaggaca agaggcccga
    25621 aaggctttta cagaactggc caaagccttt caagcaagcc atccagaatg gcaactccaa
    25681 cagactagtc agtggatgaa tcaggcccag tgtttgagac cacatttttg gatttatcta
    25741 cagagagacg gacaagtgac agaacctatg atggccttac gtttgtatgg aaatccttct
    25801 gattttggta tttccttaga ggtcagtttc atcgaacata agaaggatga gcaaacactg
    25861 ggcaagcagg ccaaagtttt agacattcca accgttaaag ggatttatta tctaacctac
    25921 tctaatggtc aaagtcaacg gtgggaggcg aatgaagaaa agcgtcgtac tttacgcgag
    25981 aaggtgagaa gtcaagaagt tcgaaaagtt ttagtgaagg tagatgttcc tatgacagaa
    26041 aattcgtctg aagaagaaat cgtagaaggc ttattgaagt cttattctaa aattcttccc
    26101 tattatctag ctacgagaaa ataagataat ttgtaaaaca tcataaatca tacagtccaa
    26161 gagtgaacag tccgctgtgt aattcttggt ctttttgttt gcgctttcgc attatataat
    26221 aaacttacaa aaacaattca aaaggagaac aattatggaa gtcgtttcaa gtgttctaaa
    26281 ttggttttct agcaatattt tgcagaatcc cgcatttttc gtaggtttat tggtgttgat
    26341 aggatatgca cttttgaaaa aacctgccca tgacgttttt tcagggtttg ttaaagcaac
    26401 agtagggtat atgttgctta acgtgggtgc tggtggtttg gttacaacct ttcgtccaat
    26461 cttagcagct cttaactaca aattccaaat tggtgcagcg gttatcgacc cttactttgg
    26521 acttgctgca gcaaacaaca aaattgcagc agagtttcca gattttgttg gaactgcaac
    26581 tacagctcta ttgattggtt ttggaataaa tatcttgctc gtagctcttc gaaagattac
    26641 gaaggtaaga accctcttta ttactggtca catcatggta caacaagctg caacagtatc
    26701 tcttatggtt ctattcttag taccacaatt gcgcaatgct tacggtacag cagcgattgg
    26761 tatcatctgt ggactttact gggcagttag ttcaaatatg actgttgagg caactcaacg
    26821 cttgactggt ggtggaggat ttgcgattgg tcaccaacag caatttgcaa tctggtttgt
    26881 agataaagta gcaggacgct ttggtaagaa agaagaaagt ttagacaatc ttaaattacc
    26941 taagttcctc tcaatcttcc acgatacagt tgttgcatct gctaccttga tgctcgtatt
    27001 cttcggggcc attcttttaa tcttgggtcc agacattatg tctaataaag aagtcatcac
    27061 ttcaggaact ctattcaatc ctgctaaaca agatttcttt atgtacatta tccaaacagc
    27121 ctttaccttc tcagtttact tgttcgtttt gatgcaaggt gtccgaatgt tcgtatctga
    27181 gttaacaaac gctttccaag gtatttcaaa caaattgttg ccaggttcat tcccagcggt
    27241 tgacgttgca gcttcttatg gatttggttc tccaaatgct gtcttgtcag gatttacctt
    27301 tggtttgatt ggtcaattga ttacaattgt cttgctcatc gtctttaaaa atccgattct
    27361 tattattaca ggatttgtac cagtgttctt tgacaatgca gccattgcgg tctacgctga
    27421 taaacgcggc ggatggaaag cggctgttat cctttccttt atatcaggtg tccttcaagt
    27481 tgctctagga gctctttgtg tggcccttct cgatttggca tcttatggtg gctaccatgg
    27541 aaatatcgac tttgaattcc catggcttgg atttggatat atcttcaaat accttggtat
    27601 tgttggttat gtacttgtgt gtctcttctt gcttgttatt cctcaacttc aatttgccaa
    27661 agcaaaagat aaagagaaat attacaacgg tgaagttcaa gaagaagctt agtatctaga
    27721 aaaggagaaa taaaatggtt aaagtattag cagcgtgcgg aaatggaatg ggttcatcaa
    27781 tggttatcaa gatgaaggtt gaaaatgctc tccgtaagct taatcaaaca gattttacag
    27841 tcaattcatg cagtgtcggt gaagctaaag gtttagcagt aggatatgac atcgtaatcg
    27901 cttctcttca tttgattcaa gaattggaag ggcgaactaa tgggaagtta attggacttg
    27961 ataacttgat ggatgataaa gaaatcaccg aaaaactcag tcaagcacta cagtaaaagg
    28021 ttggaggggg ctggacagaa actgagagtt atcgtttctg tccttctccc tctttaaata
    28081 aaggaggcag atatgaattt aaaacaagct ttaattgaca atgactcgat ccgactaggt
    28141 ttagaggcta acaattggaa agaagcagtc aaggtagcag tagatccctt aattgaaagt
    28201 ggggcaattt tgccagagta ttacgatgct atcattgaat cgactgaaga gtatgggcct
    28261 tactatatct tgatgccagg tatggctatg ccccacgcta gacctgaagc tggtgtgcaa
    28321 agtgatgcct tttcattgat taccttacaa aatcctgttg tattttcaga tgggaaagag
    28381 gtatctgttt tgttggcact agcagcaaca agttcaaaaa ttcacacaag tgtagccatt
    28441 ccacaaatta ttgccctgtt tgaattagaa gattctattg cacgtttaca ggcttgccag
    28501 actaaagaag atgtcttggc tatgattgaa gaatctaagg atagccctta tctcgaagga
    28561 ttggatttgg aaagttagaa agaggaataa agaaatgaca aaaagaatac ctaatttaca
    28621 agttgcatta gaccattcag acttgcaagg agcgattaaa gcagctgttt ctgttggtca
    28681 ggaagtagat attatcgaag ctggaactgt ttgcttgctt caagttggaa gtgaactggc
    28741 tgaagtcttg cgtagccttt tcccagataa gattattgtg gcagacacaa aatgtgctga
    28801 tgctggtgga acagttgcta aaaataatgc ggttcgtgga gcagactgga tgacttgtat
    28861 ctgttgtgca accatcccta ctatggaagc agctctaaag gctatcaaga ctgaacgagg
    28921 agaacgaggc gaaatccaga tcgagcttta tggcgattgg acttttgaac aagctcagct
    28981 ttggctagat gcaggtatct cacaagctat ttatcaccaa tctcgtgatg ctcttcttgc
    29041 tggtgaaact tggggtgaaa aagaccttaa taaggttaaa aaactcattg acatgggctt
    29101 ccgtgtatct gtaacaggtg gtctagatgt agatactctc aaactctttg aaggtgttga
    29161 tatctttacc tttatcgcag gtcgtggaat tacagaggct gcggatccag caggagcagc
    29221 gcgtgccttc aaggatgaaa tcaaacgaat ttgggggtaa atcatggtac gtccaattgg
    29281 aatttatgaa aaggcaaccc caacacactt tacttggcta gaacgtttaa attttgccaa
    29341 ggagttaggc tttgattttg tcgagatgtc tattgacgaa cgtgacgagc gtttagcaag
    29401 acttgactgg agtaaggaag aacgcttgga agttgtcaaa gcaatctatg aaactggtgt
    29461 tcgtattcct tctatctgtt tttcaggcca tcgtcgctac ccattgggtt caaaagatcc
    29521 agttctagag gaaaaatctc tagaactcat gaaaaaatgt atcgaattag ctcaagactt
    29581 gggagttcgt acgattcaat tagctggtta cgatgtttac tatgaggaaa agtcacccca
    29641 gacacgccaa cgttttatca aaaatttgag aaaagcctgt gactgggctg aagaagctca
    29701 ggtggtactt gctattgaaa ttatggatga tcctttcatc agtagcatcg aaaaatattt
    29761 ggctatagaa aaagagattg actctccctt cctctttgta tatccagata ttggtaatgt
    29821 gtctgcatgg cataatgata tctatagtga gttttatctt ggtcatcatg ccatcgcagc
    29881 tctccatctc aaggatactt atgcagtgac agaaagttca aagggccagt tccgagatgt
    29941 acctttcggg caaggttgtg tcaaatggga agaagctttc gatattttaa aggaaaccaa
    30001 ttataatgga cctttcctaa tcgaaatgtg gtctgaaaat tgtgaaacag tagaagaaac
    30061 acgcgcagcc attcaagagg cgcaagcttt tctctatcca ctcattaaga aagcaggttt
    30121 gatgtaagat gaatcaagta atcaatgcta tgcgtaaacg agtctgtgat gccaatcaat
    30181 cattgccaaa acatggactt gtcaaattta cctgggggaa tgtatctgaa gtcaatcgcg
    30241 aactcggtgt cattgttatc aaaccatcag gcgtggatta tgacgaattg acacctgaaa
    30301 acatggtagt gactgatcta gatggtaaga tcctagaagg ggatttaaga ccatcttccg
    30361 acctcccaac tcatgtgcaa ttatataagg cttggtcaga aattggtagt gtggttcaca
    30421 cccattcgac agaagctgtt ggttgggctc aggcaggtcg tgatattcct ttctacggaa
    30481 caacccatgc agattatttc tacggttcaa tcccttgcgc ccgtagtttg accaaggacg
    30541 aagtagaagt ggcctatgaa aaagatactg gcctggttat cgtagaagag tttgaacatc
    30601 gcggacttaa cccggttgaa gtaccaggaa ttgttgtacg caatcacggt ccattcacct
    30661 ggggcaaaaa tccagaggat gctgtttatc actctgtcgt actagaggaa gtatcaaaga
    30721 tgaatcgctt tacagaacaa atcaattcaa gagttgaacc tgctccccag tacatactag
    30781 agaaacacta ccaacgtaaa catggaccaa atgcttatta tggtcaaaag taagaacgat
    30841 gaaggaggag aaaaagataa atttagctcc tctttttaca tttgattttt attgagagta
    30901 aagttggagt tgaagtaatt ttgaaagatt ttttagaaat agcgcttgat atatatatgg
    30961 taaaataaaa agaattgctg tgatatcaat agatttgggg gattttttaa tatggtactg
    31021 gataaggcaa gttgtgattt gcttcaatat ttgatggatc aagaaacgtc caaaacgatt
    31081 atggcgattt cgaaagattt gaaagagtca agaaggaaaa tttattatca cattgacaaa
    31141 atcaatgctg ctctggttga cgaggcgctt cacatcatta gtattccacg aattggtatt
    31201 cacttaacgg aagagcagag agatgcttgt tgtaaactat tatcggaagt agattcgtac
    31261 gattatatca tgagtgcgca tgaacgtatg atgataatgt tactatggat aggtatttct
    31321 aaagaacgta ttacgattga aaaattgata gagttaacag aggtatctag gaatactgtt
    31381 ctcaatgatt tgaatagcat tcgttatcaa ctaactttgg aacaatatca ggtgaccttg
    31441 caagtgagca agtcacaggg ataccacctt catgcccacc ctcttaataa aattcagtat
    31501 cttcaatcgc ttctatatca tatttttatg gaagaaaatg ccacttttgt atctatttta
    31561 gaagataaga tgaaagagag gttagatgat gagtgtttgc tttctgttga aatgaaccaa
    31621 ttttttaagg aacaggttcc tttagttgaa caagatttag ggaagaaaat aaaccatcat
    31681 gaaataactt ttatgttgca ggttctacct tatttgctgt taagctgtca taatgttgaa
    31741 cagtatcaag aaagacatca ggatatagag aaagaatttt ctttgataag aaaaagaata
    31801 gagtatcagg tgtctaagaa attaggagaa cggttgtttc aaaagtttga aatttctttg
    31861 tcaggacttg aagtttctct tgtagctgtt ctcctcctct cctatcgtaa agatttggat
    31921 attcatgcag aaagtgatga ttttcgtcaa ttaaaacttg ctttagaaga atttatctgg
    31981 tattttgaat cacaaatccg aatggagatt gagaacaagg atgatttgtt acgaaatttg
    32041 atgatccact gtaaagcctt gttatttaga aagacttacg gtattttttc taaaaatcct
    32101 ctaacaaaac aaattcgatc caagtatgga gaattatttt tagtcactag aaaatctgcg
    32161 gaaattttag aaggagcatg gtttattcgg ctaacagacg atgatattgc ctatttgacg
    32221 attcatattg gaggattttt aaagtataca ccatcgtctc aaaaaaatat gaaaaaagtt
    32281 tatctcgttt gtgatgaagg tgttgcggtt tcgagacttt tgctgaaaca atgcaaactt
    32341 tattttccaa atgagcaaat tgacactgta tttacaacag aacaatttaa gagtgtggaa
    32401 gatattgcac aagttgatgt agtgattact actaatgatg atttggatag cagatttccg
    32461 attttaaggg ttaatcctat ccttgaagca gaagatattt tgaaaatgct agactatctt
    32521 aaacacaata tatttcgtaa taagagcaaa agtttcagtg aaaatctttc tagtcttatt
    32581 tcgtcttata ttgtagacag caagttggct agtaagttcc aagaagaggt tcaaacactt
    32641 ataaatcaag aaatagtagt tcaagctttt ttggaagata tttgaaggac agtccaatga
    32701 tgaacacaaa cctgtgtttt tcttggtctt ttttagtgtt ttgaagggtg gtatactaat
    32761 ctcaaagata acaattatat ccaaaggagg caacatatgc caaacgtcaa agaaattaca
    32821 agagagtcat ggattttagc cactttccca gagtggggaa catggttgaa cgaagaaatc
    32881 gaagaagaag tcgtacctga aggcaacttt gccatgtggt ggctaggcaa ctgtggtact
    32941 tggattaaga caccagctgg tgctaacgtt gtcatggacc tttggtcaaa ccgtggaaaa
    33001 tcaaccaaaa aagtgaaaga tatggttcgt gggcaccaaa tggcaaatat ggcaggtgtt
    33061 cgtaagttgc aaccaaactt gcgtgttcag ccaatggtta tcgatccatt tgctatcaac
    33121 gaactagact attacttagt ttcacacttc cacagtgatc atatcgaccc atacacagct
    33181 gcagcaattc tcaacaatcc taagttagag catgttaagt ttatcggtcc ttaccactgt
    33241 ggacgaatct gggaaggatg gggtgttcca aaagaacgta tcatcgttgt taaaccaggt
    33301 gacactatcg aattaaaaga tatgaagatt catgcagtag aatcatttga ccgtacttgc
    33361 ttggtaactc tcccagtgaa cggtgctgat gagacaggcg gtgaacttgc tggcttggct
    33421 gttacagatg aagaaatggc tcaaaaggct gttaactata tctttgaaac accaggtgga
    33481 accatctatc atggtgcaga ttctcacttc tcaaactatt ttgcaaaaca tggtaaagac
    33541 tttaaaattg atgttgcttt gaataactat ggtgaaaatc cggtaggtat ccaagacaaa
    33601 atgacatcta tcgaccttct tcgtatggca gaaaatctgc gtaccaaagt cattatccca
    33661 gttcactatg atatctggtc taacttcatg gcttctacta atgagattct agaactttgg
    33721 aaaatgcgaa aagatcgctt gcaatacgat ttccatccat ttatctggga agttggcggt
    33781 aagtacactt atcctcaaga tcaacactta gtagaatacc atcatccacg tggttttgat
    33841 gattgttttg aacaagactc taacattcaa tttaaagctt tgctataata taaaaatatt
    33901 ctttggaggt tatccgtatg ttgcatgata cggataattt tcatataata gtaaaataga
    33961 atgtgtgatt caataatcac ctcaaataga aaggaaattc tatgtcaaat ctatctgtta
    34021 atgcaattcg ttttctaggt attgacgcca ttaataaagc caactcaggt catccaggtg
    34081 tggttatggg agcggctccg atggcttaca gcctctttac aaaacaactt catatcaatc
    34141 cagctcaacc aaactggatt aaccgcgacc gctttattct ttcagcaggt catggttcaa
    34201 tgctccttta tgctcttctt cacctttctg gttttgaaga tgtcagcatg gatgagatta
    34261 agagcttccg tcaatggggt tcaaaaacac caggtcaccc agaatttggt catacggcag
    34321 ggattgatgc tacgacaggt cctctagggc aagggatttc aactgctact ggttttgccc
    34381 aagcagaacg tttcttggca gccaaatata accgtgaagg ctacaatatc tttgaccact
    34441 atacttacgt tatctgtgga gacggagact tgatggaagg tgtctcaagc gaggcagctt
    34501 catatgcagg cttgcaaaaa cttgataagt tggttgttct ttatgattca aatgatatca
    34561 acttggatgg tgagacaaag gattccttta cagaaagtgt tcgtgaccgt tacaatgcct
    34621 acggttggca tactgccttg gttgaaaatg gaacagactt ggaagccatc catgctgcta
    34681 tcgaaacagc aaaagcttca ggcaagccat ctttgattga agtgaagacg gttattggat
    34741 acggttctcc aaacaaacaa ggaactaatg ctgtacacgg cgcccctctt ggagcagatg
    34801 aaactgcatc aactcgccaa gccctcggtt gggactacga accatttgaa attccagaac
    34861 aagtatatgc tgatttcaaa gaacatgttg cagaccgtgg cgcatcagct tatcaagctt
    34921 ggactaaatt agttgcagat tataaagaag ctcatccaga actggctgca gaagtagaag
    34981 ccatcatcga cggacgtgat ccagtcgaag tgactccagc agacttccca gctttagaaa
    35041 atggtttttc tcaagcaact cgtaattcaa gtcaagatgc cttgaatgtt gtggcagcta
    35101 aattaccaac cttcctaggt ggatcagctg accttgctca ctcaaacatg acttatatca
    35161 agacggatgg acttcaagac gataccaatc gcttgaatcg taacattcag tttggtgttc
    35221 gtgaatttgc aatgggaaca atcttgaacg ggatggccct tcatggtgga cttcgtgtat
    35281 acggtggaac tttcttcgtc ttctctgact atgtgaaggc agctgtccgc ttgtcagcct
    35341 tacaaggact tcctgtgact tatgtcttta cccatgattc aatcgcagtt ggggaagatg
    35401 gtccgactca cgaaccagtt gagcatttag caggtcttcg tgctatgcca aatctaaatg
    35461 ttttccgtcc agcagatgcg cgtgaaacgc aagcagcttg gtaccttgca gtgacaagtg
    35521 agaaaacacc aactgccctt gtcttgacac gtcaaaactt gactgttgaa gatggaacag
    35581 acttcgacaa ggttgctaaa ggtgcttatg ttgtatatga aaatgcagcc gactttgata
    35641 ccatcttgat tgcgacaggt tcagaggtta atcttgctgt ctcagctgct aaagaattgg
    35701 ctagtcaagg cgaaaaaatc cgcgtagtca gcatgccatc tacagatgtc tttgataaac
    35761 aagatgcagc ttacaaggaa gagattcttc caaatgcagt ccgccgtcgt gttgcagtcg
    35821 aaatgggtgc aagtcaaaac tggtacaaat atgttggtct cgatggtgcc gttctaggta
    35881 ttgatacctt cggagcctct gccccagcac caaaagtatt ggcagaatat ggctttactg
    35941 tagaaaatct tgtaaaaatt gttcgaaact tgaaataatc ctaaaaatc
//
LOCUS       DAVLVP010000011        35684 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.011, whole genome shotgun sequence.
ACCESSION   DAVLVP010000011 DAVLVP010000000
VERSION     DAVLVP010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 35684)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35684)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35684
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.011"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(221..1855)
                     /locus_tag="VYN69_000536"
     CDS             complement(221..1855)
                     /locus_tag="VYN69_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001237556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532853.1"
                     /translation="MRLKVIKKLVDINILYSSQEANLVNLRKKQAKNPGKKVNVSARV
                     LSSYIFSSLLMLICFSNIAIHFPFEEIPIYFSSMVAILLVIAFSTSLTAFYNVFYESK
                     DLVSYRPYAFKESEIIIAKGLSVLLPALTGIVPILAYFLVLYIRLAPSLWLGLPLMLL
                     SLTLLFVSVALVMVVAVHFLAQTRVFRKYQSIFSNVMIGIGVLIPLIFVFFLQSTFGS
                     IVDKVRDIPFLLYPLHIFYKIAVEPFSTEALVGLLAWIALTVFLLYLTKKKVLPRFYD
                     VILLNSEEKVKKERRSKERISTTKKGFFRMVLRYHLTLLGQGTGVITVLFTSTFLPYL
                     MMISLISKIRDSQIVPDIHPPYWLPLFFVGVFIAVVNNNITSLHSIALSLERENVDFL
                     KSLPFDFARYVKVKFWIIFAVQSFLPVLTLLGLSLYLGLSILSMIYLLVVWIIASVIL
                     SCHHYFKDVKNLSTNWSSITDLVNRSNGIVAIVLLFIYSAILMALVIGSIFLVQSLSP
                     ILAINLGVGALIVLLALAIFGYHYYLSRILAEIEKR"
     gene            complement(1836..2585)
                     /locus_tag="VYN69_000537"
     CDS             complement(1836..2585)
                     /locus_tag="VYN69_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1532854.1"
                     /translation="MIEFQNVSKLYGDKEALSNLNLQIENGEIMGLIGHNGAGKSTTI
                     KSLVSIISPSSGRILVDGQELSENRLAIKRKIGYVADSPDLFLRLTANEFWELIASSY
                     DLSRSDLEASLARLLNVFDFAENRYQVIETLSHGMRQKVFVIGALLSDPDIWVLDEPL
                     TGLDPQAAFDLKQMMKEHAQKGKTVLFSTHVLEVAEQVCDRIAILKKGHLIYCGSVED
                     LRKDYPAQSLESIYLSLAGRKEEVADASQGH"
     gene            2764..4218
                     /locus_tag="VYN69_000538"
     CDS             2764..4218
                     /locus_tag="VYN69_000538"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1532855.1"
                     /translation="MQNQTLMQYFEWYLPHDGQHWTRLAENAPHLAHLGISHVWMPPA
                     FKATNEKDVGYGVYDLFDLGEFNQKGTVRTKYGFKEDYLQAIQALKAQGIQPMADVVL
                     NHKAAADHREAFQVIEVDPVDRTVELGEPFTINGWTSFTFDGRQDTYNGFHWHWYHFT
                     GTDYDAKRSKSGIYLIQGDNKGWANEELVDNENGNYDYLMYADLDFKHPEVIQNIYDW
                     ADWFMETTGVAGFRLDAVKHIDSFFMRNFIRDMKEKYGDDFYVFGEFWNPDKEANLDY
                     LEKTEEHFDLVDVRLHQNLFEASQAGANYDLRGIFTDSLVELKPDKAVTFVDNHDTQR
                     GQALESTVEEWFKPAAYALILLRQDGLPCVFYGDYYGISGQYAQEDFKEILDRLLAIR
                     KDLAYGEQNDYFDHANCIGWVRSGAENQSPIAVLISNDQENSKSMFVGQEWTNQTFVD
                     LLGNHQGQVTIDEEGYGQFPVSARSVSVWAVNTI"
     gene            complement(4308..6926)
                     /gene="alaS"
                     /locus_tag="VYN69_000539"
     CDS             complement(4308..6926)
                     /gene="alaS"
                     /locus_tag="VYN69_000539"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012130635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEV1532856.1"
                     /translation="MKQLSSAQVRQMWLDFWATKGHSVEPSVSLVPVNDPTLLWINSG
                     VATLKKYFDGTIIPENPRITNAQKAIRTNDIENVGKTARHHTMFEMLGNFSIGDYFRD
                     EAITWAYELLTSPEWFDFPAEKLYMTYYPDDKDSYNRWIEVGVDPSHLIPIEDNFWEI
                     GAGPSGPDTEIFFDRGEAFDPENIGLRLLAEDIENDRYIEIWNIVLSQFNADPAVPRS
                     EYKELPHKNIDTGAGLERLVAVIQGAKTNFETDLFMPIIREVEKLSGKVYDQDGDNMS
                     FKVIADHIRSLSFAIGDGALPGNEGRGYVLRRLLRRASMHGQKLGINEPFLYKLVPTV
                     GKIMESYYPEVLEKRDFIEKIVKSEEESFARTLHSGQHFAQGIVADLKEKGQSVIAGS
                     DVFKLYDTYGFPVELTEEIAEEAAMTVDREGFEAAMKEQQERARASAVKGGSMGMQNE
                     TLQNITVESVFNYNASQLSSKLVAIVADNAEVEAVSEGTASLIFAETSFYAEMGGQVA
                     DHGQILDESGKVVATVTNVQKAPNGQALHTVEVLAPLALNQEYTLAIDSNRRHRVMKN
                     HTATHLLHAALHNILGNHATQAGSLNEVEFLRFDFTHFQAVTAEELRAIEQQVNEKIW
                     EALEVKTVETDIDTAKEMGAMALFGEKYGKEVRVVTIGDYSIELCGGTHVGNTSEIGL
                     FKIVKEEGIGSGTRRILAVTGKEAFEAYREQEDALKAVAATLKAPQVKEVPHKVEGLQ
                     EQLRQLQKENAELKEKAAAAAAGDIFKDVKEVNGHRYIASQVSVSDAGALRTFADNWK
                     QKDYSDLLVLVAAIGDKVNVLVASKTKDLHAGNLVKELAPIIDGRGGGKPDMAMAGGS
                     NQAKIQELLDAVAGKL"
     gene            complement(6948..7433)
                     /locus_tag="VYN69_000540"
     CDS             complement(6948..7433)
                     /locus_tag="VYN69_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000847073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LURP-one-related family protein"
                     /protein_id="HEV1532857.1"
                     /translation="MKTFLVKQKFRLGGERFAIKDDRGEIAYQVEGSFFKIPKTFTIY
                     DAAGEQVSQISKEILTLLPRFEIQLRDGSSFVIRKKLTFWRDKYEFDNLGLRIEGNIW
                     DLNFKLLDDRDQLIAEIKKELFHLTSTYTVTVLEDAYADLVISLCVAIDYVEMLESQS
                     H"
     gene            complement(7708..7947)
                     /locus_tag="VYN69_000541"
     CDS             complement(7708..7947)
                     /locus_tag="VYN69_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000784186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532858.1"
                     /translation="MKMSTFFKKSFWPTFMIVNQTAILFHLKDGLDRQYLTTESIYWV
                     IGTFIFGNILVAVFSNMKIWDKKKNGSKKKYILKK"
     gene            complement(8200..9270)
                     /locus_tag="VYN69_000542"
     CDS             complement(8200..9270)
                     /locus_tag="VYN69_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1532859.1"
                     /translation="MKKIYSFLAGIAAIILVLWGIATHLDSKINSRDSQKLVIYNWGD
                     YIDPELLTQFTEETGIQVQYETFDSNEAMYTKIKQGGTTYDIAIPSEYMINKMKDEDL
                     LVPLDYSKIEGIENIGPEFLNQSFDPGNKFSIPYFWGTLGIVYNETMVDEAPEHWDDL
                     WKPEYKNSIMLFDGAREVLGLGLNSLGYSLNSKDLQQLEETVDKLYKLTPNIKAIVAD
                     EMKGYMIQNNVAIGVTFSGEASQMLEKNENLRYVVPTEASNLWFDNMVIPKTVKNQNS
                     AYAFINFMLKPENALQNAEYVGYSTPNLPAKELLPEETKEDKAFYPDVETMKHLEVYE
                     KFDHKWTGKYSDLFLQFKMYRK"
     gene            complement(9267..10040)
                     /locus_tag="VYN69_000543"
     CDS             complement(9267..10040)
                     /locus_tag="VYN69_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1532860.1"
                     /translation="MKKFANLYLGLVFLVLYLPIFYLIGYAFNAGDDMNSFTGFSWTH
                     FETMFGDGRLMLILAQTFFLAFLSALIATIIGTFGAIYIYQSRKKYQEAFLSLNNILM
                     VAPDVMIGASFLILFTQLKFSLGFLTVLSSHVAFSIPIVVLMVLPRLKEMNGDMIHAA
                     YDLGASQFQMFKEIMLPYLTPSIIAGYFMAFTYSLDDFAVTFFVTGNGFSTLSVEIYS
                     RARKGISLEINALSALVFLFSIILVVGYYFISREKEEQA"
     gene            complement(10037..10843)
                     /locus_tag="VYN69_000544"
     CDS             complement(10037..10843)
                     /locus_tag="VYN69_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1532861.1"
                     /translation="MKKTSSKLFVVPYMLWIALFVLAPLVLIFGQSFFNIEGQFSLEN
                     YKSYFASQNLTYLKMSFNSVLYAGIVTFVTLLISYPTALFLTRLKHRQLWLMLIILPT
                     WINLLLKAYAFIGIFGQNGSINQFLEFIGIGSQQLLFTDFSFIFVASYIELPFMILPI
                     FNVLDDMDNNLINASYDLGATKWETFRHVIFPLSMNGVRSGVQSVFIPSLSLFMLTRL
                     IGGNRVITLGTAIEQNFLTNDNYGMGSTIGVILILTMFITMWVTKERRER"
     gene            complement(10824..11981)
                     /locus_tag="VYN69_000545"
     CDS             complement(10824..11981)
                     /locus_tag="VYN69_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018372333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1532862.1"
                     /translation="MKKPIIEFKNVSKVFEDSNTKVLKDINFELEEGKFYTLLGASGS
                     GKSTILNIIAGLLDATTGDIMLDGVRINDIPTNKRDVHTVFQSYALFPHMNVFENVAF
                     PLRLRKIDKKEIEQRVAEVLKMVQLEGYEKRSIRKLSGGQRQRVAIARAIINQPRVVL
                     LDEPLSALDLKLRTDMQYELRELQQRLGITFVFVTHDQEEALAMSDWIFVMNDGEIVQ
                     SGTPVDIYDEPINHFVATFIGESNILPGTMIEDYLVEFNGKRFEAVDGGMKPNEPVEV
                     VIRPEDLRITLPEEGKLQVKVDTQLFRGVHYEIIAYDELGNEWMIHSTRKAIVGEEIG
                     LDFESEDIHIMRLNETEEEFDARIEEYVEIEEQEAGLINAIEEERDEENKL"
     gene            complement(12141..13046)
                     /gene="murB"
                     /locus_tag="VYN69_000546"
     CDS             complement(12141..13046)
                     /gene="murB"
                     /locus_tag="VYN69_000546"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEV1532863.1"
                     /translation="MSVREKMLEILEGIDIRFKEPLHSYSYTKVGGEADYLVFPRNRF
                     ELARLVKFANQENIPWMVLGNASNIIVRDGGIRGFVILCDKLNNVSVDGYTIEAEAGA
                     NLIETTRIALRHSLTGFEFACGIPGSVGGAVFMNAGAYGGEIAHILQSCKVLTKDGEI
                     ETLSAKDLAFGYRHSAIQESGAVVLSVKFALAPGTHQVIKQEMDRLTHLRELKQPLEY
                     PSCGSVFKRPVGHFAGQLISEAGLKGYRIGGVEVSEKHAGFMINVADGTAKDYEDLIQ
                     SVIEKVKEHSGITLEREVRILGESK"
     gene            complement(13158..13910)
                     /locus_tag="VYN69_000547"
     CDS             complement(13158..13910)
                     /locus_tag="VYN69_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000152219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family intramembrane metalloprotease"
                     /protein_id="HEV1532864.1"
                     /translation="MTIHIIITMLLLLAFLIGSIWFAKKKYQINLAVLGLGAVAFFVS
                     SQILEKLVHILILHPQKDGSIALLQDHPLIYIIYGLAMAAFFEETARLVFFKWLEKKR
                     SLEKADALAYGLGHGGLELIFLGLISLLNLYIVLSAVQTQNPQVMQLLSENMLKTIQS
                     LSVWQIYLLGFERILALGFQLLLTVWVYQAVRQKKWIYLLAAYGLHAFFDLAPSFFQV
                     GWLTNPVLVEVILALELVLVAYGTKEIFCKKS"
     gene            complement(13920..14651)
                     /gene="budA"
                     /locus_tag="VYN69_000548"
     CDS             complement(13920..14651)
                     /gene="budA"
                     /locus_tag="VYN69_000548"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEV1532865.1"
                     /translation="MDKNVQEPVKLFQYNTLGALMAGLYGGTMTVGELLEHGDLGLGT
                     LDSIDGELIVLDGKAYQAKGSGDQPEIVEVSPDALIPYAAVVPHQAEVIFRQRFEMTD
                     KELEERIESYYDGENLFRSIKIRGEFLHMHVRMIPKSTPDTKFADVATHQPEYSRDNV
                     AGTIVGFWTPEIFHGVSVAGYHLHFISDDLTFGGHVMDFVIKEGIIEVGAVDQLDQRF
                     PVQDRQYLFAKFNVDEMKKDIEKAE"
     gene            14793..16064
                     /locus_tag="VYN69_000549"
     CDS             14793..16064
                     /locus_tag="VYN69_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEV1532866.1"
                     /translation="MKKQSKYKEVVSYLKNGIESGRFPTGSRLPSIRQLSLDFHCSKD
                     TIQRALLELRHEQYLYAKPQSGYYVLEQGQHQDLEIEVTDEHASAYDDFRLCVNETLI
                     GRENYLFNYYDNQEGLEDLRQSIHKLLFEQALYCKANQLVLTSGTQQALFILSQISFP
                     SQAKEILVEQPTYHRMNRLLIAQGLDYQTIERGIDGIDLKELERHFKTGKIKFFYTIP
                     RFHYPLGHSYSEQDKRSILNLAAKYDVYIVEDDYLGDLDSKKGQTFHYLDTEEHVIYI
                     KSFSTSLFPALRITALILPNAIKEAFVAYKNILDYDSNLIMQKALSLYIDSQLFEKNR
                     LARLTNHESYQKQIEERITKTPCPLPHYPLHDGLLLDLRQYPKIASLKHSQPGLDFFE
                     EAYLSTCPYQFAKVSLDNLENVLNYLKAELE"
     gene            complement(16084..16881)
                     /locus_tag="VYN69_000550"
     CDS             complement(16084..16881)
                     /locus_tag="VYN69_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1532867.1"
                     /translation="MFVLCNFLASFFLVACQSGSNGSQSAVEAIKQKGKLVVATSPDY
                     APFEFQSLVDGKNQVVGADIDMAQAIADELGVKLEISSMSFDNVLTSLQTGKADLAVA
                     GISATDERKEVFDFSIPYYENKISFLVRKADVEKYKDLTSLESANIAAQKGTVPESMV
                     KEQLPKAQLTSLTNMGEAVNELQAGKVDAVHMDEPVALSYAAKNAGLAVATVSLKMKD
                     GDANAVALRKNSDDLKEVVDKVIQKLKDEGIYQSYLEKAASLTEVEE"
     gene            complement(17057..17710)
                     /gene="phoU"
                     /locus_tag="VYN69_000551"
     CDS             complement(17057..17710)
                     /gene="phoU"
                     /locus_tag="VYN69_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000946475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEV1532868.1"
                     /translation="MLRSQFEEDLEKLHNQFYAMGQEVLSQINRTVRAFVTHDRDLAK
                     EVIEDDAEVNEYEVKLEKKSFEMIALQQPVSQDLRTVLTVLKAVSDVERMGDHAVAIA
                     QATIRMKGEERIPAVEEEIKKMGREVKSVVEAALDLYLNGSVDDAYRVASMDEQINHY
                     FETIRDLATEEIKKNPEAIVTGRDYFQVISYLERIGDYAKNICEWVVYFETGKIVEL"
     gene            complement(17722..18480)
                     /gene="pstB"
                     /locus_tag="VYN69_000552"
     CDS             complement(17722..18480)
                     /gene="pstB"
                     /locus_tag="VYN69_000552"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1532869.1"
                     /translation="MTDAILQVSDLSVYYNKKKALNSVSLSFQPKEITALIGPSGSGK
                     STLLKSLNRMGDLNPEVTTTGSVVYNGHNIYSPRTDTVELRKEIGMVFQQPNPFPMTI
                     YENVVYGLRINGIKDKQVLDEAVEKALQGASIWDEVKDRLYDSAIGLSGGQQQRVCVA
                     RVLATSPKIILLDEPTSALDPISAGKIEETLYGLKDKYTMLLVTRSMQQASRISDKTG
                     FFLDGDLIEFNDTKQMFLDPQHKETEDYITGKFG"
     gene            complement(18493..19296)
                     /gene="pstB"
                     /locus_tag="VYN69_000553"
     CDS             complement(18493..19296)
                     /gene="pstB"
                     /locus_tag="VYN69_000553"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000852852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEV1532870.1"
                     /translation="MSTYNWDEKHILTFPEEKVALSTKDVHVYYGKNESIKGIDMQFE
                     RNKITALIGPSGSGKSTYLRSLNRMNDTIDIAKVTGQILYRGIDVNRPEINVYEMRKH
                     IGMVFQRPNPFAKSIYRNITFAHERAGVKDKQVLDEIVETSLRQAALWDQVKDDLHKS
                     ALTLSGGQQQRLCIARAISVKPDILLMDEPASALDPIATMQLEETMFELKKNFTIIIV
                     THNMQQAARASDYTGFFYLGDLIEYDKTATIFQNAKLQSTNDYVSGHFG"
     gene            complement(19307..20191)
                     /gene="pstA"
                     /locus_tag="VYN69_000554"
     CDS             complement(19307..20191)
                     /gene="pstA"
                     /locus_tag="VYN69_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009443446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEV1532871.1"
                     /translation="MRAKKLDKLATAVLYTIASIIVTILASLILYILVRGLPHISWSF
                     LTGRSSAFQAGGGIGIQLYNSFFLLVITLIISVPLSMGAGIYLAEYAKKGPVTNFVRT
                     CIEILSSLPSVVVGLFGYLIFVVQFEYGFSIISGALALTVFNLPQMTRNVEDSLKHVH
                     HTQREAGLALGISRWETVVHVVIPEALPGIVTGVVLASGRIFGEAAALIYTAGQSAPA
                     LDWSNWNILSVTSPISIFRQAETLAVHIWKVNSEGTIPDGTIVSAGSAAVLLIFILIF
                     NFGARKFGSYLHKKLTAA"
     gene            complement(20181..21098)
                     /gene="pstC"
                     /locus_tag="VYN69_000555"
     CDS             complement(20181..21098)
                     /gene="pstC"
                     /locus_tag="VYN69_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018378778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEV1532872.1"
                     /translation="MNQEELAKKMLLPSKNSRLEKLGKGLTFACLSLIVILVAMILVF
                     VAQKGLSTFFVNGVNIFDFLLGGTWNPSSKEFGALPMILGSFIVTILSALIATPFAIG
                     AAVFMTEVSPKGAKILQPAIELLVGIPSVVYGFIGLQVVVPFVRSVFGGTGFGILSGI
                     SVLFVMILPTVTFMTTDSLRAVPRYYREASFAMGATRWQTIWRVILKAARSGIFTAVV
                     FGMARAFGEALAIQMVVGNSAVIPTSLTTPAATLTSILTMGIGNTVMGTVNNNVLWSL
                     ALVLLLMSLVFNSVIKLITKERGKKNYAR"
     gene            complement(21098..21976)
                     /gene="pstS"
                     /locus_tag="VYN69_000556"
     CDS             complement(21098..21976)
                     /gene="pstS"
                     /locus_tag="VYN69_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEV1532873.1"
                     /translation="MKKRKKLALSLIAFWLTACLVGCASWIDRGESITAVGSTALQPL
                     VEVAADEFGTIHVGKTVNVQGGGSGTGLSQVQSGAVDIGNSDVFAEEKDGIDASALVD
                     HKVAVAGLALIVNKEVDVDNLTTEQLRQIFIGEVTNWKEVGGKDLPISVINRAAGSGS
                     RATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDDSVKSMKLNGYD
                     LSPENISSNNWPLWSYEHMYTLGQPNELAAEFLNFVLSDETQEGIVKGLKYIPIKEMK
                     VEKDAAGTVTVLEGRQ"
     gene            complement(22560..23864)
                     /locus_tag="VYN69_000557"
     CDS             complement(22560..23864)
                     /locus_tag="VYN69_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001160253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmF rRNA methyltransferase first C-terminal
                     domain-containing protein"
                     /protein_id="HEV1532874.1"
                     /translation="MQFPEGFVEKYKEILGDEARDFLASFEEEAVSAFRVNPLKEEQL
                     SFSDAITQTPWGHYGKVSGKSPEHATGLVYSQEPAAQMVAQVAQPSPGMKVLDLAAAP
                     GGKSTQLAAYLAGEGLLVSNEISSKRAKILVENMERFGATNVVVTNESADRLVKVFKG
                     YFDLIVLDAPCSGEGMFRKQPDAMDYWSLDYPSQCASLQREILEDAVTMLAEGGHLVY
                     STCTWAPEENEEIVNWLLEEYDFDLLPVEHINGMVAGIDLPETARMYPHQFKGEGQFV
                     AHLQFKGNNPAPKFKASKSNLSREQVALWQEFAQNHLKVNLPGILQTFGDQLYLLPEL
                     LPDLGKLKIARNGLHLGTFKKKRFEPSFALGLALKPSQVEQSVEIGQEAFVKYAAGEI
                     VQLAESLPNGWYQVLVKGNGLGFAKVTGNVLKNYFPKGLRFK"
     gene            complement(23865..24638)
                     /locus_tag="VYN69_000558"
     CDS             complement(23865..24638)
                     /locus_tag="VYN69_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEV1532875.1"
                     /translation="MESKFEFAKELVKKAGQYILDHMQEDLRVETKSSPTDLVTRLDK
                     EVQELLVGEILSRYPEDKICAEEGCLRASVQEGKVWVIDPIDGTNNFVAQQEDFAVMM
                     AYFENGQGQFGLIYDVVKGDCYHGGGEFPVCLNDRSLAPFKTKPLGDFLIAGNSGMLE
                     TNEWGLADLSRSVLGVRVYGSAAISFAKILSGRLLTYLTYLQPWDYAAASILGESLGY
                     RVVTLFGEAPDFQTRQPVMMVPLEMQEEIQSYIYERKRT"
     gene            complement(24628..24906)
                     /locus_tag="VYN69_000559"
     CDS             complement(24628..24906)
                     /locus_tag="VYN69_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEV1532876.1"
                     /translation="MNKQYSYPLDLSWSTEELASVLSFFNDVEAAYEGKVEAKKLLDS
                     YKGFKAVVPSKSEEKRLGREFETVSGYSLYRAVQAAKEKGEGKISLGK"
     gene            complement(24906..25307)
                     /locus_tag="VYN69_000560"
     CDS             complement(24906..25307)
                     /locus_tag="VYN69_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007556665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEV1532877.1"
                     /translation="MITLFLSPSCTSCRKAKAWLEKHKVPFVEHNIMTSPLTRKELQH
                     ILSLTENGTDDIISTRSKIFQKLNIDVESISVSELLHLIEQYPSLLRRPIIIDAKRMQ
                     IGFNEDEIRAFLPRSYRKQELKEARMRAGIS"
     gene            complement(25517..26134)
                     /locus_tag="VYN69_000561"
     CDS             complement(25517..26134)
                     /locus_tag="VYN69_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEV1532878.1"
                     /translation="MKTKLIFWGSMLFLLSLSILLTIYLAWIFYPMEIQWLNLTNRVY
                     LKPETIQYNFHILMNYLTNPFSQVLQMPDFRSSAAGLHHFAVVKNLFHLVQLVALVTL
                     PSFYVFVNRIVKKDFLSLYRKSLLALVVLPVMIGLGGVLIGFDQFFTLFHQILFVGDD
                     TWLFDPAKDPVIMILPETFFLHAFLLFFALYENFFGYLCLKSRRK"
     gene            complement(26124..26897)
                     /locus_tag="VYN69_000562"
     CDS             complement(26124..26897)
                     /locus_tag="VYN69_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEV1532879.1"
                     /translation="MKYKGYLIDLDGTIYKGKDRIPAGETFVHELQKRDIPYLFVTNN
                     TTRTPESVKEMLAQNFNIDTPLSTVYTATLATIDYMNDLGLEKTVYVVGEAGLKEAIK
                     AAGYVEDKEKPAYVVVGLDWQVDYEKFATATLAIQKGAHFIGTNPDLNIPTERGLLPG
                     AGSLITLLEVATRVKPVYIGKPNAIIMDKAVEHLGLEREELIMVGDNYLTDIRAGIDN
                     GIPTLLVTTGFTKAEEVAGLPIAPTHVVSSLAEWDFDEN"
     gene            complement(26908..27645)
                     /locus_tag="VYN69_000563"
     CDS             complement(26908..27645)
                     /locus_tag="VYN69_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000524807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEV1532880.1"
                     /translation="MGLTYQMKMKIPFDMADMNGHIKLPDVILLSLQVSGMQSIELGV
                     SDKAILEEYNLVWIIVEYDIEVFRLPRFAEEITIETEALSYNRLFCYRRFTIYNEAEQ
                     ELIHMMATFVLMDRDSRKVHVVEPEIVAPYQSDFDKKLIRGPKYESLEEPISKDYHVR
                     FYDLDMNGHVNNSKYLDWIFEVMGADFLTHYIPKKINLKYVKEVRPGGVITSAVERTG
                     LESKHEITSDGVINAQAIITWQEIKKA"
     gene            complement(27650..28780)
                     /locus_tag="VYN69_000564"
     CDS             complement(27650..28780)
                     /locus_tag="VYN69_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEV1532881.1"
                     /translation="MQKKPTSAYVHIPFCTQICYYCDFSKVFIKNQPVDSYLEHLLEE
                     FRSYDIEKLSTLYIGGGTPTALSAPQLEVLLNGLTKNLDLSVLEELTIEANPGDLDAD
                     KIAVLKNSAVNRVSLGVQTFDDKMLKKIGRSHLEKDIYENIDRLKLAGFDNISIDLIY
                     ALPGQTMEQVKENVAKAIGLDIPHMSLYSLILENHTVFMNRMRRGKLPLPKEELEAEM
                     FEYIIAELERAGFEHYEISNFSKPGFESRHNLMYWDNAEYYGIGAGASGYVNGVRYKN
                     HGPIRHYLSAVEEGNACITEDHLSQKEQMEEEMFLGLRKKSGVSMARFEEKFGQSFAG
                     LYGEIVRDLVQQGLMQIEGDHVRMTKRGLFLGDTVAERFILE"
     gene            complement(28864..29253)
                     /locus_tag="VYN69_000565"
     CDS             complement(28864..29253)
                     /locus_tag="VYN69_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEV1532882.1"
                     /translation="MGKLSSILLGTVSGAALALFLTSDKGKQVCSQAQDFLDDLREDP
                     EYAKEQVCEKLTEVKEQATDFVLKTKEQVESGEITVDSILAQAKSYAFQATEASKNQL
                     NNLKEQWQEKAEALDDSEEIVIDITEE"
     gene            complement(29269..29652)
                     /locus_tag="VYN69_000566"
     CDS             complement(29269..29652)
                     /locus_tag="VYN69_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEV1532883.1"
                     /translation="MLEVAYILVALAVIVFLVYLIITVQKLGRVIDETEKTIKTLTSD
                     VDVTLHHTNELLAKVNVLADDINVKVATIDPLFSAVADLSLSVSDLNDHARVLSKKAS
                     SAGSKTLKTGASLSALRLASKFFKK"
     gene            complement(29653..30441)
                     /locus_tag="VYN69_000567"
     CDS             complement(29653..30441)
                     /locus_tag="VYN69_000567"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEV1532884.1"
                     /translation="MLDPIAIQLGPLAIRWYALCIVTGLILAVYLTMKEAPRKKIIPD
                     DILDFILVAFPLAILGARLYYVIFRFDYYSQNLGEIFAIWNGGLAIYGGLITGALVLY
                     IFADRKLINTWDFLDIAAPSVMIAQSLGRWGNFFNQEAYGATVDNLDYLPGFIRDQMY
                     IEGSYHQPTFLYESLWNLLGFALILIFRRKWKSLRRGHITAFYLIWYGFGRMVIEGMR
                     TDSLMFFGLRVSQWLSVVFIGLGIMIVIYQNRKKAPYYITEEEN"
     gene            complement(30434..31369)
                     /locus_tag="VYN69_000568"
     CDS             complement(30434..31369)
                     /locus_tag="VYN69_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEV1532885.1"
                     /translation="MSVLVKEVIEKLRLDIVYGEPELLEKEINTADITRPGLEMTGYF
                     DYYTPERIQLLGMKEWSYLISMPSNSRYEVLKKMFLPETPAVIVARGLVVPEEMLKAA
                     RECKIAILTSRAATSRLSGELSSYLDSRLAERTSVHGVLMDIYGMGVLIQGDSGIGKS
                     ETGLELVKRGHRLVADDRVDIFAKDEITLWGEPAEILKHLIEIRGVGIIDVMSLYGAS
                     AVKDSSQVQLAVYLENYDTHKTFDRLGNNAEELEVSGVAIPRIRIPVKTGRNISVVIE
                     AAAMNYRAKEMGFDATRLFDERLTSLIARNEVQNA"
     gene            complement(31499..31675)
                     /locus_tag="VYN69_000569"
     CDS             complement(31499..31675)
                     /locus_tag="VYN69_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000048061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEV1532886.1"
                     /translation="MSKTVVRKNESLDDALRRFKRAVTKAGTLQETRKREFYEKPSVK
                     RKRKSEVARKRKKF"
     gene            complement(31815..32522)
                     /locus_tag="VYN69_000570"
     CDS             complement(31815..32522)
                     /locus_tag="VYN69_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEV1532887.1"
                     /translation="MKVIKVENQVQGGKVAFEILKEKLANGAQTLGLATGSSPLEFYK
                     EIVESDLDFSNLTSVNLDEYVGLDGDNPQSYRYFMQENLFNQKPFKESFLPRGVKDNA
                     EAEVERYNQILADHPVDLQILGIGRNGHIGFNEPGTPFDSQTHLVELDQSTIEANARF
                     FAKIEDVPTQAISMGIKNILDAKSIILFAYGESKAEAIAGTVSGPVTENLPASSLQNH
                     PDVTIIADAEALSLLEK"
     gene            32673..33701
                     /gene="queA"
                     /locus_tag="VYN69_000571"
     CDS             32673..33701
                     /gene="queA"
                     /locus_tag="VYN69_000571"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEV1532888.1"
                     /translation="MNTADFDFHLPEELIAQTPLEKRDASKLLIVNRETGEMQDKHFH
                     SIIDMLEPGDALVMNDTRVLPARLYGQKVETGGHVELLLLKNTSGDEWEVLAKPAKRL
                     KVGTRISFGDGRLSAVVTEELTHGGRIVRFEYQGIFLEVLESLGEMPLPPYIHEKLDD
                     RERYQTVYAKESGSAAAPTAGLHFTKELLAEIQAKGVHLVYLTLHVGLGTFRPVSVDN
                     LDEHEMHSEFYQLSEEAAATLRSVKENGGRVIAVGTTSIRTLETIGSKFDGQIQADSG
                     WTNIFIKPGYEWKVVDAFSTNFHLPKSTLVMLVSAFAGRELVLDAYHHAIQEHYRFFS
                     FGDAMFIY"
     gene            complement(34362..34751)
                     /locus_tag="VYN69_000572"
     CDS             complement(34362..34751)
                     /locus_tag="VYN69_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1532889.1"
                     /translation="MVKRRIRRGTREPEKVVVPEQSSIPSYPVSVTSNQGTDVAVEPA
                     KAVAPTTGWKQENGMWYFYNTDGSMATGWVQVNGSWYYLNSNGSMKVNQWFQVGGKWY
                     YVNASGELAVNTSIDGYRVNDNGEWVR"
     gene            complement(34805..35497)
                     /locus_tag="VYN69_000573"
     CDS             complement(34805..35497)
                     /locus_tag="VYN69_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1532890.1"
                     /translation="MNTSFVHAADGIQYVRDDTRDKEEGIEYDDADNGDIIVKVATKP
                     KVVTKKISSTRIRYEKDETKDRSENPVTIDGEDGYVTTTRTYDVNPETGHVTEQVTVD
                     RKEATDTVIKVPAKSKVEEVFVPFATKYEADNDLSAGQEQEITLGKNGKTVTTITYDV
                     DGKSGQVTESTLSQKEDSQTRVVKKGTKPQVLVQEIPIETEYLDGPTLDKSQEVEEVG
                     EIGKLLLLQSIL"
BASE COUNT        10648 a         8149 c         6739 g        10148 t
ORIGIN      
        1 tacttggatc acacacatta taggaattaa cgttttttga aattgaaaaa tatcccaata
       61 aataaactat aaacaacaaa aatagaacta tattatattt cttattcaaa acattcctcc
      121 ctatattttt gattaccaat cttaatcatt tacaactaca ttctaacaaa ctataaaagc
      181 gtttgtcgaa ttgaatttat caagcaagca accaactatc tcatcttttt tctatttctg
      241 ccaatatgcg tgacaggtaa taatgatagc caaaaatagc aagagcaagc aagacgataa
      301 gagctcctac tcccaagttg atggcaagga taggggagag agactgaacc aagaatatgc
      361 tcccaattac aagggccatc aggattgcac tataaataaa caataaaact atggcgacta
      421 tgccatttga acgattcacc aggtccgtaa tgctactcca attggttgac agatttttaa
      481 cgtccttaaa gtaatggtgg caagaaagga tgacactggc aatgatccag actacaagaa
      541 ggtaaatcat cgaaaggatg gacaagccta gatatagaga aagaccgagc aaagtcagaa
      601 ctggtaaaaa ggactgaaca gcaaagataa tccaaaattt cactttcaca taacgagcaa
      661 agtcaaaggg taaactcttc agaaaatcaa cattttccct ctccaaggac aaggcaattg
      721 aatgcaggct ggtgatattg ttattgacaa ctgctataaa cactcctaca aaaaacaagg
      781 gtaaccagta tggaggatga atgtctggaa ctatctgaga atctcggatt ttggaaatca
      841 gactgatcat catgagataa ggaaggaaag tacttgtaaa aagcactgta atcacgccag
      901 tcccctgtcc caagagggtg aggtggtagc gtaaaaccat gcggaaaaat ccctttttag
      961 tagttgaaat cctctccttg ctgcgacgtt cttttttgac cttctcctca ctgttaagca
     1021 ggatcacgtc ataaaaacga ggaagaacct tctttttggt caggtaaagc aagaaaacag
     1081 ttaaagctat ccaagcgagc agacccacta aggcttctgt cgaaaaaggc tccactgcta
     1141 ttttgtaaaa gatatgaaga ggataaagga gaaatggaat gtctctaact ttgtcaacaa
     1201 tacttccaaa agtcgactgt agaaagaaga caaatattaa aggtatgaga actcctatcc
     1261 caatcatcac attcgaaaaa atagactgat actttctgaa gaccctagtc tgagccaaga
     1321 aatgcactgc cactaccatc actagagcca cagagacaaa taataaggtc aaggacagta
     1381 gcatcaaagg caaacccagc catagagaag gagctagcct aatgtagagg actagaaaat
     1441 aagctaggat tggtacaatt ccagttagag ctggcaagag gacagacagt cctttagcaa
     1501 ttataatctc tgattcttta aaggcatagg gcctatacga taccaaatcc ttactctcat
     1561 aaaagacatt gtaaaaggcc gttaaagaag ttgaaaaggc aatcactagt aaaatagcaa
     1621 ccatcgagct aaaataaata ggtatttcct caaaaggaaa atgaatggct atattactaa
     1681 aacagatgag catcaagaga ctggaaaaaa tgtaagaact taggactcta gcggaaacat
     1741 ttactttttt cccaggattc ttagcctgct tctttcgtag attaaccaga ttagcttctt
     1801 gagatgaata aaggatattg atatcaacta attttttaat gaccttgaga cgcatccgca
     1861 acctcctctt ttctaccagc aagactaagg tagatacttt ccaaagactg ggctgggtaa
     1921 tcttttctca agtcctctac actaccacaa taaatcaaat gccccttttt caaaatggca
     1981 atccgatcac agacttgctc tgccacctct aggacatgag ttgaaaacaa gactgtcttc
     2041 cctttttgtg catgttcctt catcatctgt ttcaaatcaa aggcagcctg gggatccaaa
     2101 ccagtcaaag gttcatccaa gacccaaata tcgggatcag acaagagtgc tccgatgaca
     2161 aagactttct gacgcattcc gtgagaaaga gtttcaataa cctgatagcg attttcagca
     2221 aaatcaaaaa cgttcaatag cctagctaga ctagcctcca agtcagatct actcagatca
     2281 taggatgagg cgatcaattc ccaaaattca ttggccgtta agcgtaaaaa taagtcaggc
     2341 gagtctgcta cgtagccaat ctttcgttta atagccaagc gattttccga taactcctga
     2401 ccgtctacca aaatacgacc actgctgggt gaaatgatac tgactaggga ttttatagtg
     2461 gtcgattttc cagccccatt atgaccaatc aagcccataa tctctccatt ttcaatctgc
     2521 aaattgagat tactcaaggc ctctttatca ccatacaact tactaacatt ttgaaattca
     2581 atcatgggac aactcctatc tttatctgta ttatacatcg ctatactagc actttgatac
     2641 aaaaaaatca atactttatt ttaagtagtt aaaatagttt ccttctatct tacgcaaacg
     2701 tttgcgccga gagatacttt atgatagaat aaagaacaag attgacaagt aagaggaaac
     2761 atcatgcaaa atcaaacact catgcaatac tttgaatggt atctgcccca cgacggtcaa
     2821 cactggacgc gtctggctga aaatgctcca cacctagctc atctggggat cagtcacgtc
     2881 tggatgccac cagccttcaa ggcaaccaac gaaaaagatg tcggctatgg ggtctatgac
     2941 ttatttgact taggagagtt caaccaaaaa gggactgtcc gcaccaagta tggtttcaaa
     3001 gaagactatc ttcaagccat tcaagccctt aaagcacagg gaattcaacc tatggccgat
     3061 gtagttctca accacaaggc tgctgccgat cacagggaag cctttcaggt catcgaagtt
     3121 gatcctgtag accgtacagt tgaacttgga gaacccttca ccatcaatgg ctggactagt
     3181 tttaccttcg atggtcgcca agatacctat aatggcttcc actggcattg gtaccacttc
     3241 accggtacag actacgatgc caaacgcagt aaatctggga tttatctgat ccaaggggac
     3301 aacaagggct gggctaacga ggaattggtc gataacgaaa acggaaacta cgactacctc
     3361 atgtatgccg acctagactt taaacatcct gaagtcatcc aaaacatcta tgactgggct
     3421 gattggttca tggaaacgac tggtgtagct ggtttccgtt tggatgccgt taagcatatt
     3481 gactctttct ttatgcgcaa cttcatccgc gatatgaagg aaaaatacgg tgacgatttc
     3541 tatgtttttg gtgaattttg gaacccagac aaggaagcca atctggacta tctcgaaaaa
     3601 acggaagaac actttgacct tgtcgatgtt cgtctccatc agaatctctt tgaagccagt
     3661 caagctggcg caaactatga ccttcgtggc attttcacag atagcctggt tgaactcaag
     3721 cctgacaagg ctgtgacttt tgtcgacaac cacgataccc aacgaggaca agcccttgag
     3781 tctaccgttg aagaatggtt caagccagca gcctatgccc tcattttgtt acgccaagac
     3841 ggccttccat gtgtctttta cggagactac tatgggattt caggccagta tgctcaagaa
     3901 gatttcaaag aaatccttga ccgcctccta gccatccgaa aagacttggc ctatggagaa
     3961 caaaatgact actttgacca tgctaactgt atcggttggg tacgttcagg tgctgaaaat
     4021 caatccccaa tcgcagtcct tatctcaaat gaccaagaaa acagcaagtc aatgtttgtc
     4081 ggtcaagaat ggactaatca aacctttgta gatttacttg gtaaccacca aggtcaagtt
     4141 acaattgatg aggaaggtta tggacaattc cctgtctcag ctagatccgt aagtgtctgg
     4201 gcagtcaata ccatctaata gctcataata accaagctag gtccaagcgg atttggcttt
     4261 tttgtaatca agtaaagacc tacccaaatg gatagatctt tacttgatta caatttacct
     4321 gctactgcat ccaacaattc ttggatctta gcttggttgc ttcctcctgc catggccatg
     4381 tctggtttac caccaccacg tccatcgatg attggtgcta attctttgac aaggtttcct
     4441 gcatgaaggt cttttgtctt gcttgctaca aggacattga ctttgtcacc gatagcagca
     4501 actaggacaa gaagatcaga gtagtctttt tgtttccagt tatctgcaaa ggtacgaagg
     4561 gcaccggcat ctgatacaga cacttgacta gcaatgtagc ggtgaccgtt gacttccttc
     4621 acatccttga agatatcgcc tgcggctgca gctgcggctt tttctttcaa ctcagcattt
     4681 tctttttgaa gttgacgaag ttgttcttga agtccttcta ccttgtgagg tacttccttg
     4741 acttgaggtg ctttcaaggt tgctgcaaca gctttaagag cgtcctcttg ttcacgatag
     4801 gcttcaaagg cttccttacc agtcactgcc aagatacgac gagttcctga accgattcct
     4861 tcttctttga caattttgaa gagaccaatc tcagaagtgt tgccaacgtg ggtaccacca
     4921 caaagttcaa tagagtagtc accgatagtc acgacacgaa cttccttgcc gtatttctca
     4981 ccaaagaggg ccatcgctcc catttcttta gcagtgtcaa tatccgtttc aactgtctta
     5041 acttcaagag cttcccagat tttttcgttg acttgctgtt caatcgcacg aagttcctca
     5101 gcagttactg cttggaagtg ggtaaagtca aagcgaagga attcaacttc gttaagagat
     5161 cctgcctgtg ttgcgtggtt tccaaggata ttgtgaaggg cagcgtgaag caaatgagtc
     5221 gcagtgtggt ttttcatgac acggtgacgg cgattgctat caattgccaa ggtatattct
     5281 tggttcaagg caagcggtgc aaggacttca actgtatgaa gggcttgacc atttggggct
     5341 ttctgaacat tggtcacagt agccacaacc ttacctgact catccaagat ttgtccgtgg
     5401 tcagctacct gtccacccat ttcagcataa aatgacgttt ccgcaaagat aagagaggca
     5461 gttccttctg aaacagcttc tacttctgca ttgtccgcca cgatagctac caatttagaa
     5521 gacaattggc tagcattgta gttgaagaca ctttctacag tgatgttttg aagagtttca
     5581 ttttgcatac ccattgagcc acccttgaca gctgacgcac gcgcgcgttc ttgctgttct
     5641 ttcatggctg cttcaaaacc ttcacggtct acagtcatag cagcttcttc agcgatttct
     5701 tcagtcaatt caactgggaa cccataagta tcatagagtt tgaagacatc tgaaccagcg
     5761 ataacagatt gacctttttc tttcaagtct gctacaatgc cttgggcaaa gtgttgacct
     5821 gagtgtaggg tacgagcaaa tgactcttcc tcgctcttaa cgattttttc aataaagtca
     5881 cgtttttcaa gcacttctgg gtagtagctt tccatgattt ttccaaccgt tggaacaagt
     5941 ttgtaaagga aaggctcgtt gatacccaat ttttgaccat gcatagaagc acgacggaga
     6001 agacggcgaa ggacgtaacc acgaccttca tttcctggaa gggcaccatc accgatggca
     6061 aatgaaagtg aacggatgtg gtctgcgatg accttaaagc tcatgttgtc gccatcttgg
     6121 tcataaacct taccagacaa tttctccact tcacgaatga ttggcatgaa gaggtctgtt
     6181 tcaaagttag tcttagcacc ttggataacc gctaccaaac gctccaaacc agcgcccgta
     6241 tcaatgttct tatgtggcaa ttctttgtac tcgctacgag gaacagcagg gtctgcgtta
     6301 aattgtgaca aaacgatgtt ccagatttca atataacggt cgttttcaat atcttctgca
     6361 agcaggcgaa gaccgatatt ttctgggtca aaagcttccc cacggtcaaa gaagatttct
     6421 gtatctggtc cagaaggtcc cgcaccaatt tcccagaagt tgtcctcaat tggaatcaag
     6481 tgacttggat ccactcccac ttcaatccag cggttgtaag aatctttatc gtctggatag
     6541 taggtcatgt aaagtttttc agcagggaaa tcaaaccatt cagggcttgt caaaagctca
     6601 taagcccaag tgatagcttc gtcacggaag taatccccga tagagaagtt ccccagcatt
     6661 tcaaacatgg tatggtgacg cgcagtcttc cctacgtttt cgatgtcgtt ggtacggata
     6721 gccttttggg cattggtaat acgtggattt tcagggataa tggtcccgtc aaagtatttc
     6781 ttaagggttg ctaccccaga gttgatccac aaaagagttg ggtcatttac aggaaccaaa
     6841 cttactgatg gttctactga gtgacctttg gtcgcccaga aatcaagcca catttggcgt
     6901 acttgtgcac tagatagttg tttcatattg tctccttatt cacttgttta atgtgattgg
     6961 ctttccagca tttccacata gtcaatcgcg acacagaggg aaatgactag gtctgcataa
     7021 gcgtcttcaa gaaccgttac ggtataggta gaggtcagat ggaagagttc cttcttaatt
     7081 tccgcaatca actgatcgcg atcatccagc aatttgaaat tcaaatccca gatattgccc
     7141 tcgatacgaa gacctagatt atcaaactca tacttatctc gccagaaggt caacttctta
     7201 cgaatgacaa aactcgagcc atcccgaagc tgaatctcaa aacgaggaag caaggtcaag
     7261 atttctttac tgatctgact gacttgttca ccagccgcat catagatggt aaaagttttg
     7321 ggaatcttaa aaaatgatcc ctccacctga taggcaattt ctcccctgtc atccttgata
     7381 gcgaagcgtt cgcctccaag acgaaacttt tgtttgacaa gaaatgtttt catcaacacc
     7441 tccaaaaatc aaaagacaag ctcatatcac gaagggcgaa aaaccgcggt accaccttca
     7501 ttcaatgaac ttgtcattct cttgttctta tgcaattgta tgattgagta gcatgacttc
     7561 ctagcttaga tggctcgcag caccgccatt tctctggact aagacaagtg aaaatcaatt
     7621 ctcaactttc ttattataac gtttttttaa gcttgcgtca actggaaatg atctccgttg
     7681 aattagacca attccctaca tctctgatta ctttttcagg atatattttt tcttactgcc
     7741 atttttcttt ttatcccaaa ttttcatatt actaaacaca gctactagaa tatttccaaa
     7801 tataaaggtg cctatcaccc aatatatgga ctcagttgtt aggtattgtc gatccaagcc
     7861 atcctttaaa tggaatagta tagcagtttg gttaacaatc ataaaggttg gccagaaact
     7921 ttttttgaaa aaagtagaca ttttcattat ttgttgccgc tttctgtaag gttaatactc
     7981 aataaaaatc aaaaagcaaa ctaggaagct agcctcaagc tgtacttgag tacggcaagg
     8041 caacgctgac gtggtttgaa gagtataggc ttagtatact actaggcaag caaataaaca
     8101 aataaacaac tagaatagaa aaagataggg ctctaaaaac tgacttctat tccttaaaaa
     8161 cgaaccagct tgactgattc gtcttcttac gtttatctcc tacttccgat acattttaaa
     8221 ctgtaggaag aggtcgctat atttccctgt ccatttatgg tcaaatttct cataaacttc
     8281 taggtgtttc atggtttcaa catcgggata gaaggcctta tcttcctttg tttcctctgg
     8341 gagcaattcc ttcgctggta ggtttggtgt tgaatagccg acatactccg cattttggag
     8401 agcattttca ggtttcaaca taaagttgat aaaggcataa gctgagtttt ggtttttaac
     8461 tgttttggga atgaccatat tgtcaaacca aagattgctg gcctctgtcg gtaccacata
     8521 acgtagattt tcattttttt ctaacatttg gctggcttca ccagagaagg tcacgccgat
     8581 tgcaacatta ttctgaatca tatagccctt catctcgtcc gcaacgatag ccttgatatt
     8641 tggagtcagt ttgtagagct tatccactgt ctcttccaac tgctgcagat ccttggagtt
     8701 gaggctgtag ccgagggaat tgagtcctag tcccagcacc tcacgcgccc catcaaagag
     8761 catgatagaa ttcttatact ccggcttcca aaggtcatcc caatgctcag gcgcttcatc
     8821 taccatggtt tcgttgtaga caattcctaa ggttccccag aagtaaggga tggagaattt
     8881 attacctggg tcaaaggact ggttgagaaa ctctggtccg atattttcga ttccttcaat
     8941 ttttgaataa tcaagcggaa ccaagaggtc ttcgtccttc atcttgttaa tcatgtattc
     9001 acttggaatg gcaatatcgt aggtcgttcc accctgcttt atcttagtat acatggcttc
     9061 gttggagtca aaagtctcgt actgaacttg aattcctgtt tcttctgtaa actgagtcaa
     9121 gagttcagga tcgatatagt ctccccagtt atagataacc aatttttgac tatctcgact
     9181 attgatttta ctatctaaat gagtcgcaat tccccacaag acaaggataa tcgctgcaat
     9241 tcctgctaaa aatgaataga tttttttcat gcttgctcct ccttctcacg agagataaag
     9301 taataaccta caactaggat aatactaaag agaaagacta gagcagacag ggcattgatt
     9361 tctaaggaaa tccctttccg tgcacgagag taaatctcga ctgacagggt tgaaaagcca
     9421 tttcctgtta caaagaaggt cacggcaaaa tcatccagcg aataggtgaa ggccatgaag
     9481 taacctgcaa tgatagacgg agtcaggtaa ggaagcatga tttccttaaa catctgaaat
     9541 tgactagctc ccaagtcata ggccgcatga atcatgtcgc cattcatttc cttgagtcga
     9601 ggcaagacca tcaagaccac gataggaatg gagaaggcca cgtgactaga tagaacggtc
     9661 aaaaagccaa gtgaaaactt gagttgggta aagagaatca agaagctagc accaatcata
     9721 acgtcaggcg caaccatgag gatattattg agtgatagaa aggcttcttg gtatttctta
     9781 cgagactggt agatgtaaat ggcaccaaaa gtcccgataa tggtcgctat caaggctgat
     9841 aggaaggcca agaaaaatgt ctgagccaaa atcagcatga gtctcccatc tccaaacatg
     9901 gtttcaaagt gagtccagct aaaacctgta aagctattca tatcatcacc agcattaaag
     9961 gcatagccaa tcaagtaaaa gataggcagg tagaggacca gaaagaccag tcccagataa
    10021 aggttggcaa attttttcat cgttctctcc tttccttagt cacccacatg gtgatgaaca
    10081 tggtcaggat gagaatcaca ccgatggttg aacccatacc atagttgtca ttggttagaa
    10141 aattctgctc aatagccgtc cccaaggtga taacgcggtt cccaccaatc aaacgggtca
    10201 gcatgaagag actcaaactt gggataaaga ccgactgaac cccacttcgc acaccgttca
    10261 tagatagagg gaagatgaca tgacggaagg tctcccactt agttgcacca aggtcataac
    10321 tagcattgat gagattatta tccatatcgt ctaagacatt gaaaatcggc aaaatcataa
    10381 aggggagctc gatgtagctt gcgacaaaga taaaggaaaa atcggtaaaa agcaactgtt
    10441 gtgaaccaat tccgataaat tccaagaatt ggttaataga gccattttga ccaaaaatcc
    10501 cgataaaagc ataggcttta aggagcaaat tgatccaggt aggaaggata atcagcatga
    10561 gccagagttg acggtgcttg agacgggtca aaaagagggc tgttggatag ctgataagca
    10621 aggtcacaaa ggtcacaatc cctgcataaa gcactgagtt gaaactcatt ttgaggtagg
    10681 tcaagttttg tgacgcaaag taagatttat aattttctaa actaaactgg ccttcaatgt
    10741 tgaaaaagga ttgtccgaaa atcaagacca agggtgccaa tacaaagagg gcaatccaaa
    10801 gcatgtaggg cactacaaag agtttagagc ttgttttctt catctctttc ctcctcgatc
    10861 gcattgatca gacctgcttc ttgctcttcg atttccacgt attcttcgat acgagcatcg
    10921 aactcttctt cggtttcatt gagacgcatg atgtggatgt cttctgattc aaagtccaga
    10981 ccgatttcct cacccacgat ggccttacgg gttgagtgga tcatccattc atttccaagt
    11041 tcgtcatagg cgataatttc ataatgaact ccacggaaga gctgggtgtc gaccttaact
    11101 tggagtttgc cttcttcagg aagggtaatg cgcaagtcct ctggacgaat aacgacctca
    11161 acaggttcat ttggcttcat cccaccatca accgcttcaa agcgtttgcc gttaaattcg
    11221 accaagtagt cctcaatcat ggtacctggc aagatgtttg actccccgat aaaggtggca
    11281 acaaagtggt tgattggctc atcgtagatg tccacagggg ttccagactg gacaatctcg
    11341 ccatcattca taacgaaaat ccagtcactc atggcaagag cttcttcctg atcgtgagtg
    11401 acaaagacaa aggtaatgcc caatcgttgt tgtaattcac gcaattcgta ctgcatgtct
    11461 gttctcaatt tcaagtccag cgctgataaa ggctcgtcca acaagaccac acggggttgg
    11521 ttgatgatag cacgggcgat ggccacacgc tgacgttgtc ctccagaaag tttgcggatg
    11581 gaacgttttt cataaccttc caactgaacc atcttgagaa cttccgctac gcgttgctcg
    11641 atttctttct tgtcaatctt gcgcaagcga agtggaaagg caacattttc aaacacattc
    11701 atatgtggaa acaaggcata ggattggaag accgtatgga catcacgctt gttggttgga
    11761 atatcattga tacgaacacc gtctagcatg atatctcctg tcgtcgcatc cagtaaacct
    11821 gcaataatgt ttaggatagt tgatttcccc gaaccagatg cacctagaag ggtgtagaat
    11881 ttcccttctt ccaactcaaa gttgatgtct ttgagaacct tggtgttgct gtcttcaaaa
    11941 actttagaga cgtttttgaa ttcgataatt ggttttttca attggcataa attccttctt
    12001 tttcatagat taacagatcg gggctctgtc aggtcgccac tacctcttgc agggagtaaa
    12061 accacctgca tacatcttcg ctaccgatag gctttcaccc cctttccatg acatagcagc
    12121 agcttttcaa atacagcttc ctacttgctt tcacccaaga tccggacttc tctttcaagc
    12181 gtaatacctg agtgttcctt gactttttcg ataaccgatt ggatcaagtc ctcgtagtct
    12241 ttggccgttc catctgcgac attgatcata aatcctgcat gcttttctga tacttctacg
    12301 ccaccgatac gatagccttt caagccagct tctgaaatta actgacctgc aaaatgcccg
    12361 actggacgct taaagaccga gccacaagat gggtattcca aaggttgctt gagttcacgt
    12421 aggtgcgtca agcggtccat ttcctgcttg ataacctgat gggttcctgg agctagggca
    12481 aatttaactg acaagacaac tgcaccagac tcctgaatag ctgaatggcg gtaaccaaaa
    12541 gccaagtctt tagcagacag ggtttcgatt tctccatcct tggtcaagac cttacaagac
    12601 tgcaagatgt gagcaatctc gccaccatag gcacccgcat tcataaagac agcaccgcca
    12661 acgcttcctg gaataccaca agcaaactca aagccagtta aactatgacg gagggcaatg
    12721 cgagttgttt caatcaagtt agccccagct tctgcttcaa tggtatagcc atcaacagaa
    12781 acgttattga gcttgtcaca caagatgaca aatccacgaa tcccaccatc acgaacgatg
    12841 atattgcttg cattgccaag aaccatccaa gggatatttt cttggttggc aaatttcaca
    12901 aggcgcgcca actcaaaacg atttcgtgga aagaccaaat aatcagcctc tccacctact
    12961 tttgtataac tatagctatg caagggttcc ttaaaacgga tatcaattcc ttctaagatt
    13021 tcaagcattt tttctcttac agacatgtca ctcttccttt tacaaaattc attccattat
    13081 accattttta gagacatttg acgaccataa aaataccttg tttggatttt gcataagaaa
    13141 aagaggttcc ccccttttta tgatttttta caaaagattt ccttggttcc ataggcaacc
    13201 agaacgagct ccagtgctag aatcacttca accaagactg gatttgtcaa ccagcctact
    13261 tggaaaaaag atggtgccag atcaaagaag gcatgcaagc cataggctgc taggagataa
    13321 atccatttct tctggcgaac agcttggtaa acccaaactg tcaaaagtaa ttggaaacca
    13381 agcgccaaga ttcgctcaaa accaagcaaa taaatctgcc agaccgaaag tgactgaatg
    13441 gtttttaaca tattttcaga cagtaattgc ataacctgtg gattctgagt ttgaactgcc
    13501 gaaagaacga tgtaaagatt gagtaaacta ataaggccta gaaaaatcaa ctccaagcca
    13561 ccatgcccta ggccataggc caaggcatct gccttttcca aacttctctt tttctccaac
    13621 catttgaaga aaacaagacg agccgtttcc tcaaaaaatg ctgccatggc taggccatag
    13681 ataatataga taagcggatg atcttgcaag agggcaatac taccgtcttt ttgaggatgc
    13741 aaaatcaaga tatgcaccag tttttccaaa atctgcgaag agacaaagaa agcgactgca
    13801 cccaagccca agacagctag attaatctgg tatttctttt tggcaaacca aatgcttcct
    13861 atcagaaaag ctagcaacag caacatggta ataatgatat gaatggtcat tttcttctcc
    13921 tattctgcct tttcaatatc ttttttcatc tcgtcaacat tgaacttagc aaacaagtat
    13981 tgacggtctt ggactgggaa acgttggtcc aactggtcaa ctgctcccac ctcgataatg
    14041 ccttccttga tgacaaaatc catgacatgt ccaccgaagg tcaaatcatc tgatatgaag
    14101 tgcagatggt agcctgccac actgacccca tggaaaatct caggcgtcca gaaaccaaca
    14161 atagtccccg caacattgtc acgactatat tccggttgat gggttgcgac atcagcaaac
    14221 ttggtatcgg gtgttgactt aggaatcata cgcacatgca tatgtaaaaa ttccccccga
    14281 atcttgatag agcggaaaag attttcccca tcataataag actcaattcg ttcttccaat
    14341 tccttgtctg tcatctcaaa gcgctgacgg aaaatgacct ctgcctgatg cggtactact
    14401 gctgcataag gaataagggc atctggtgac acttccacaa tttctggctg gtctcctgac
    14461 cctttggcct gataagcctt gccatccaag acaatcaatt ccccatcaat ggaatccaag
    14521 gttcccaaac caaggtcacc atgttctagc aattctccta ctgtcatagt accaccataa
    14581 aggccagcca tcaaggctcc aagagtattg tattgaaata atttcactgg ttcctgcacg
    14641 ttcttatcca ttcactttct tgtctgttat tctataaatc ttactcctaa gtataccaca
    14701 tttaccccta gatgtgaacg agagaaacgc tctagacatt gccaagaagg aaaaaaaaag
    14761 gtacaatgta acaaaatcaa gggaggtctg gaatgaagaa acaaagcaag tacaaagagg
    14821 tcgtttccta tctgaaaaat ggtatcgagt ctggacgatt tccgacggga agtcgcctgc
    14881 cttctatccg tcaactgagc cttgacttcc actgtagcaa ggacaccatt caacgagccc
    14941 tgctggaatt acggcacgaa caatacctct atgccaagcc ccagagtggc tactatgtcc
    15001 tagaacaagg gcaacaccag gacctagaaa tcgaggttac cgacgaacat gccagtgcct
    15061 atgacgattt ccgactctgt gtcaatgaaa ccttgattgg ccgagaaaac tacctcttca
    15121 actactatga caatcaagaa ggattagaag acctaagaca atccattcac aaactcctct
    15181 ttgagcaagc tctctactgc aaggctaacc aactagtact gacttctgga acccaacaag
    15241 ccttgtttat cctctctcaa atatcctttc ctagccaagc caaggaaatc ttggtggaac
    15301 agccaaccta ccatcggatg aatcgcctct tgattgcaca ggggctggac tatcaaacga
    15361 ttgaacgagg cattgatggg attgacctga aggagctgga gcgacacttc aagacaggaa
    15421 aaattaagtt tttctacacc attccccgat ttcactatcc tctgggacat tcctattctg
    15481 agcaagacaa acgatccatt cttaacttag ctgccaagta tgatgtctat atcgtagaag
    15541 acgattatct gggtgatttg gactccaaga agggccaaac cttccactat cttgatacag
    15601 aggagcatgt catttatatc aagtccttct cgaccagcct ttttcctgcc cttcgtatta
    15661 cagcactcat tcttccaaat gctatcaaag aagcatttgt ggcctacaaa aatatcctag
    15721 actacgacag caacctcatt atgcaaaaag ccctgtcact ctatattgac agtcaattgt
    15781 ttgaaaaaaa tcgtttggct cgcttgacca atcatgaatc ttaccaaaaa caaatcgagg
    15841 aaaggataac taaaacacct tgtccccttc ctcattatcc cctacacgat ggtttattgc
    15901 tagacctgag acagtatcct aaaatcgcca gtctcaaaca cagtcaaccg ggcttggact
    15961 tctttgaaga ggcctattta agtacctgtc cttatcaatt tgccaaggtg tccctagaca
    16021 atctggaaaa tgttttaaac tatttaaaag cagaattgga atgacatcca actctgcttt
    16081 ttcttattct tcaacttcag ttagacttgc tgctttttca agatagcttt ggtaaattcc
    16141 ttcatcctta agtttttgga tgaccttgtc taccacttct ttcaaatcat cactattttt
    16201 tctaagagca acggcattgg cgtcgccgtc cttcatcttc aagctgacag ttgcgacagc
    16261 taagccagcg tttttagcag cataactaag tgcaacaggc tcatccatat gaacagcatc
    16321 tacttttcca gcctgcaatt cattgactgc ttcacccata ttagttaggg aagttaattg
    16381 agcttttggc aattgttcct tgaccattga ttctggaaca gtcccttttt gggctgcaat
    16441 attagcactt tctaggctag ttaaatcctt gtatttttcc acatcagcct tacgaaccaa
    16501 gaaactaatc ttgttttcat agtatgggat tgaaaaatca aagacttctt ttctctcgtc
    16561 agtagcacta attcctgcaa ctgctaggtc agccttacca gtttgaagac tggtcaaaac
    16621 attgtcaaaa ctcatgcttg agatttccaa cttaacccca agttcatcag cgatagcctg
    16681 agccatgtcg atgtctgcac cgactacctg gttctttcca tcaaccaatg attgaaattc
    16741 aaagggtgca tagtcaggac tggttgccac aactaatttc cccttttgct taatggcctc
    16801 aacagctgac tgagaaccat tagaacctga ctggcaagct actaagaaga aacttgcaag
    16861 aaaattacat aaaacaaata tccatttttt ttgatttcat aaataaacaa cctctctgtt
    16921 aattttgtat aattataacg atatccaagt tacttgtcaa gtgtttttta aatttttatc
    16981 tcaaaaatat tttttcgttc aaaaaaagga gccatcagtt gatttcaagc tcctttttat
    17041 acagaattaa actattttat agttcgacaa tcttacctgt ttcaaagtag acaacccatt
    17101 cacagatatt tttagcatag tcaccgatac gctccaagta ggaaataact tggaaataat
    17161 cacgacccgt aacaatggct tctggatttt tcttaatctc ttcagtcgca aggtcacgga
    17221 tagtttcaaa atagtggtta atttgctcat ccatggaggc cacccggtat gcgtcgtcaa
    17281 cagaaccatt aagataaaga tcaagtgctg cttcaacaac gcttttaact tcacgtccca
    17341 ttttttttat ttcttcctct acagctggaa tacgttcttc ccccttcata cggatggttg
    17401 cctgggcaat ggctacagcg tgatccccca tacgctccac atctgataca gccttaagga
    17461 cagtcaagac tgtacgcaaa tcttgagaga ctggttgttg gagtgcgatc atttcaaatg
    17521 atttcttttc cagtttcact tcgtattcat ttacttctgc atcatcttcg atgacctctt
    17581 ttgccaggtc acggtcatgc gtgacaaaag cacgtaccgt acgattgatt tgtgagagca
    17641 cttcttgtcc catagcgtag aactggttat gtaatttctc taaatcttct tcaaattgag
    17701 atcgtaacat ctttcatctc cttatccaaa ttttcctgta atatagtctt ccgtttcctt
    17761 gtgttgggga tcaaggaaca tctgcttggt atcattaaat tcaatcaaat ctccatctag
    17821 gaaaaatcct gtcttatcag agatacgtga agcttgctgc atggaacggg ttaccagaag
    17881 catggtgtac ttgtctttta gaccatacaa ggtttcctca attttaccag ctgaaatcgg
    17941 atccaaagcc gaagttggct catccaagag gatgatttta ggactagttg ccaagacacg
    18001 ggccacgcag acacgctgct gttgaccacc tgacaatcca atagctgaat catatagacg
    18061 atccttgacc tcatcccaga tagaggcacc ttgcaaggct ttttctacgg cttcatccag
    18121 aacctgctta tccttaattc cattgatacg aagcccgtag acaacattct catagatagt
    18181 catagggaaa ggattaggtt gttggaaaac cattccgatt tccttacgta attcaaccgt
    18241 atctgtacgc ggactgtaga tgttgtgacc attgtacacc acggatccag ttgtggtcac
    18301 ctctggattg agatctccca tgcggttgag agacttgagg agggttgact tccctgatcc
    18361 agatggacca atcaaggctg taatttcctt aggttggaaa gatagggaaa cactattcaa
    18421 agccttcttt ttattataat aaacggacag gtctgatacc tgtaaaatcg catctgtcat
    18481 acggtttcct ttctaaccaa agtgaccaga tacatagtca ttggtggact gtagcttggc
    18541 attttggaaa atagttgcag tcttgtcata ctcaatcaaa tcacccaagt aaaagaagcc
    18601 tgtatagtca cttgcacgag cagcctgctg catattatgc gttacaatga tgatggtaaa
    18661 gtttttcttg agctcaaaca tggtctcttc tagttgcatg gtcgcaatcg gatccaaggc
    18721 tgaggctggc tcatccatta agaggatatc tggcttaaca gagatggcac gagcgataca
    18781 gagacgttgt tgctgaccac ctgataaggt caaggctgac ttgtggagat cgtctttaac
    18841 ctgatcccag agggcagcct gacgaaggga ggtttctacg atttcatcta ggacttgctt
    18901 atccttaact ccagcacgtt catgcgcaaa ggtaatatta cggtaaattg acttagcaaa
    18961 tggattgggg cgttgaaaaa ccattccaat gtgtttacgc atttcataaa cgttgatttc
    19021 tggacggttg acatcaattc cacgatagag aatctgccca gttactttag caatatcaat
    19081 agtatcattc atgcgattga gactgcgtaa gtaggtagat ttccccgatc ccgacgggcc
    19141 aatcaaagct gtaattttat ttctttcaaa ttgcatatca atccccttaa tggattcatt
    19201 tttaccatag taaacatgga catccttagt agaaagggct actttttctt caggaaaggt
    19261 aaggatatgc ttctcatccc agttatatgt tgacatggct tctcctttag gcagcggtta
    19321 atttcttgtg tagatagctt ccgaacttac gagctccaaa gttaaaaatc aggataaaga
    19381 tcaggagcac agcggcagaa cctgctgata caatggttcc atctggaata gtgccttcac
    19441 tattgacttt ccagatatgg acagccaagg tttctgcttg acggaagata gagatggggc
    19501 tagtcacact gaggatattc cagttagacc agtcaagagc tggcgccgat tgccctgctg
    19561 tatagatcag agctgcagct tcgccaaaga tacgaccaga tgccaagacg acacccgtta
    19621 caatacctgg aagcgcttct ggaataacaa catgaaccac tgtctcccag cgagaaatcc
    19681 caagagccag accagcctca cgttgggtat ggtgaacgtg tttcaaacta tcttctacat
    19741 tacgcgtcat ctgaggcaag ttaaagactg tcaaggccaa ggcacctgaa atgattgaaa
    19801 atccatactc aaactggact acaaagatca agtaaccaaa gagacccacc accactgatg
    19861 gtaaagagga caagatttca atacaagtcc gcacaaagtt ggtaacagga ccttttttag
    19921 catattcagc caagtaaatc ccagctccca tagaaagagg tacagaaata atcaaggtaa
    19981 tgaccaatag gaaaaaggaa ttgtaaagct gaatgccaat cccaccacct gcttgaaaag
    20041 cagaagacct tccagtcaag aaagaccaag agatatgggg caagccccga accaagatat
    20101 agagaatcaa ggaagccaag attgtcacaa tgatgctagc aatcgtatag aggacagctg
    20161 ttgcaagttt atctaatttc ttagcgcgca taatttttct ttcctctttc tttcgtaatc
    20221 aatttaatca cactgttaaa aactaaactc atcaagagca gtaccaaggc cagtgaccag
    20281 agaacattat tatttacagt tcccatgaca gtgttcccaa ttcccatagt taatatagaa
    20341 gttaaagttg cagctggtgt ggtcaaggaa gttgggataa cagctgagtt tccgacaacc
    20401 atctggatag ctaaagcctc accaaaggca cgcgccatcc caaagaccac tgcagtgaaa
    20461 ataccagaac gggccgcctt caagattaca cgccagatag tctgccagcg agtggctccc
    20521 atagcgaaac tggcctcacg ataataacga ggaaccgcac gcaagctatc cgttgtcata
    20581 aaggttacgg tcggcaaaat catgacaaag aggacggaaa tccctgacaa aatcccaaaa
    20641 ccagtcccac caaagacact gcgaacaaag ggaacgacga cttgcaagcc aataaatccg
    20701 tacactactg aaggaatccc aaccaggagt tcaatagctg gttgtaaaat cttcgcccct
    20761 tttggtgata cttcggtcat aaaaactgct gcaccaatag caaagggtgt tgcgataagg
    20821 gctgagagaa tggtaacgat aaaggaaccc aaaatcatag gaagggcacc aaattcttta
    20881 ctagaaggat tccaagttcc tcccaaaaga aagtcaaaga tattcacacc attgacaaag
    20941 aaggtcgaca agcctttttg cgctacgaaa accaaaatca tggccacaag gatgactatc
    21001 aaagaaagac aggcaaaggt caaacctttt cctaatttct ccagacgaga attctttgat
    21061 ggaagcaaca ttttcttagc taattcttct tgattcatta ttgtctccct tccaacactg
    21121 tcacagttcc ggcagcatct ttttcaacct tcatttcctt aatcggaata tacttcaatc
    21181 ctttgacaat cccttcttgg gtctcatccg agagaacaaa attgagaaat tctgcagcca
    21241 actcattggg ctgccccaat gtatacatat gctcataaga ccacaagggc caattattgc
    21301 tacttatatt ttctggactt aagtcatagc cattcaactt catgcttttg accgaatcat
    21361 ctatataggt aagagataaa taagagatag ctcctggact ttttgatacg attgatttta
    21421 ccgctccatt tgaatcctgc tcctgacttt gcatggcaga ctgaccttcc ataatgacag
    21481 tatcaaaggt agcacgagag ccagagccgg ctgcccgatt gataacagag atgggtaagt
    21541 ccttaccacc aacctctttc caattggtta cctcacctat gaagatttga cgaagttgct
    21601 ctgtcgttag gttatcaaca tcaacctcct tattgacaat cagagccaag ccagctaccg
    21661 cgaccttgtg gtcaacaaga gcagaagcat caattccgtc tttttcctca gcaaatacat
    21721 ctgagtttcc tatatcaact gccccagact gaacctggga caagcctgta ccagaacctc
    21781 ccccttggac attgaccgtt tttccaacat ggatcgtgcc aaattcatct gccgctactt
    21841 caaccaaggg ttgcaaggca gttgagccaa cagccgttat ggattctcca cgatcaatcc
    21901 agctagcaca gcctactaaa caagccgtca gccaaaaagc gataagagac agagcaagct
    21961 tttttctttt tttcactgtt tttctcctcg aaaataatta tgaatactgt gaatttttta
    22021 agtagttctt tatgagttga cgcatgaatt cttaccaaat ttctgcgcaa ttgattattt
    22081 atataatata ggctatatta ctctttccta acctcctttt ttcatatgtg gataaaatct
    22141 cttgtctatc ccttccccca ttgtcaccca ttatagtcat ttcgtgtctc tttttcccct
    22201 ttttaatgca agggaaatta ctctccttag atgataatcc aaaagctaga aaggtatctc
    22261 aaacctctct actctcccag actagtttac aactaaaagg aaaagattct attttatgag
    22321 aaatctagtt tacaagcggt aagaacgcta ataactacta ataactaaac ttcttgtact
    22381 ctttgaaaat ctcttcaaac cagtgttttg agctatctat ggctagcttc ctagtttgct
    22441 ctttgatttt cattgagtag taaaactaca tgtaatggca atcaagatat caagaatcat
    22501 cctactaaaa aaatccatac tttcactata acatagaata agatatttga ctagcatttt
    22561 catttgaatc tgagaccttt tggaaaataa tttttcaaaa catttccagt aacctttgca
    22621 aagcccaagc cattgccttt aaccaaaact tggtaccaac catttggcag actttctgcc
    22681 agctgaacga tttctccagc cgcatacttg acaaacgctt cttggccaat ttcaaccgac
    22741 tgttcgacct gactcggttt caaggctaaa ccaagagcga aactgggctc aaagcgtttc
    22801 ttcttaaaag tgcccagatg cagtccattg cgagcaatct tgagcttccc taaatctggc
    22861 aaaagttctg gcaagagata aagctggtct ccaaaagtct gcaagatacc cggtagattg
    22921 accttcaaat ggttttgggc aaattcctgc cacaaggcaa cttgttcacg gctgaggtta
    22981 ctcttacttg ccttaaattt aggagctgga ttgttaccct taaactgtag atgggcaaca
    23041 aactgaccct ctcccttaaa ctgatgagga tacatccgag ccgtttctgg caggtcaata
    23101 ccagctacca ttccattgat atgctctact ggcaacaagt caaaatcata ctcttccagc
    23161 aaccaattga caatctcttc gttttcctcg ggtgcccagg tacaggtcga ataaaccaga
    23221 tgaccacctt cagctaacat ggtcactgca tcctccagaa tttctctttg caagctagca
    23281 cattgactcg gataatctaa gctccaatag tccatagcat caggttgctt acgaaacatt
    23341 ccttcaccag agcaaggggc atcaagaacg attaagtcaa aatagccttt aaagaccttg
    23401 accaagcggt cggcagattc attggtcacc acgacatttg tcgctccaaa acgctccatg
    23461 ttttcaacca aaatcttagc ccgtttgctt gaaatttcat tggaaacaag tagcccctcc
    23521 cctgctagat aggctgccag ttgagttgat ttgccccccg gtgcagcagc caagtccaag
    23581 accttcatac caggactggg ttgggctact tgagccacca tttgagcagc aggttcttgc
    23641 gaataaacta aacctgtagc atgctcaggc gatttccctg aaaccttccc atagtggccc
    23701 caaggggttt gagtaatggc atcagaaaag gaaagttgct cttcttttaa gggattgacc
    23761 cgaaaggccg aaaccgcttc ctcctcaaaa gaggcaagaa aatctcttgc ctcatctcct
    23821 agtatctctt tatatttttc aacaaatcct tctggaaatt gcatttaagt tcttttcctt
    23881 tcgtaaatat aggactgaat ttcctcctgc atctcaagag gcaccatcat gaccggctgt
    23941 ctggtttgaa aatcaggagc ttcaccaaaa agggtcacaa cccgatagcc cagactttcc
    24001 cctaaaatac tagctgcggc ataatcccat ggttgcagat aagtgagata ggtcaacaaa
    24061 cgccctgaca aaatcttggc aaaactaatg gccgcacttc catagacacg aacaccaaga
    24121 accgatcggc tcaaatcagc cagcccccat tcattggttt ccagcatacc actattccct
    24181 gcaatgagaa aatctccaag tggtttagtt ttaaaaggag ctagggacct atcatttaga
    24241 caaactggaa attccccacc accgtggtaa caatcccctt tgaccacatc ataaatcaga
    24301 ccaaactgtc cctgaccatt ttcaaaataa gccatcataa cagcaaaatc ttcctgctgg
    24361 gctacaaaat tattggtacc atcaatggga tcaatgaccc aaaccttgcc ctcttgaacc
    24421 gaggctcgca gacaaccttc ttcagcacaa atcttatcct caggataacg ggacaaaatc
    24481 tcaccaacca agagttcctg aacttctttg tccagtctgg tcaccaaatc tgttggagag
    24541 gacttggttt caacacgcaa gtcttcctgc atatggtcaa gaatgtactg acctgctttc
    24601 ttaacaagct ctttagcaaa ttcaaattta ctttccaaga gaaatctttc cttccccttt
    24661 ttctttggca gcctgaactg ctcggtaaag tgaatagcca ctaaccgttt caaattctcg
    24721 tcccaaacgt ttctcttcgc tcttgcttgg cacgaccgct ttgaatccct tataggagtc
    24781 cagtaacttt ttagcctcta ctttgccttc ataggcagct tcaacatcat taaaaaaaga
    24841 aagcactgaa gcaagttctt cagtgctcca cgacaaatct agtgggtaac tatactgttt
    24901 gttcattaac taataccagc tctcattctt gcttctttta gttcttgctt acgataacta
    24961 cgagggagaa aagcacgaat ctcatcttca ttaaaaccga tttgcatacg cttggcatca
    25021 ataataattg gacgacgcaa aagactagga tactgctcaa tcaaatgaag caattccgat
    25081 accgaaatac tctctacatc aatattcaat ttttgaaaaa tttttgaacg agttgaaatg
    25141 atgtcatcag taccattttc ggtcaaggaa aggatgtgtt gcaattcttt tcttgttaaa
    25201 ggactggtca taatattgtg ttccacaaag ggaaccttat gtttttctaa ccaggcctta
    25261 gccttacgac atgatgtaca gctcggtgat agaaatagtg taatcatgct tttctcttct
    25321 tatctatact ttgctacttc tattatacaa aaaaataagg cgcttgacta gggattttta
    25381 gaaaaaaagc ctattttttc aggaaaaata ggctgtttgc gaacgattga cacaattgga
    25441 tttggttaat tcactcttaa cgatggtttt aaacgatata tatatttata tatgtaaatt
    25501 aaaaacatct ttcctttcac ttcctacgac ttttcagaca cagatagcca aagaagtttt
    25561 catagagggc aaaaaagagg aggaaggcat gaagaaagaa ggtctctggc aaaatcataa
    25621 taacaggatc cttggctgga tcaaaaagcc aggtatcatc tcccacaaag agaatttgat
    25681 ggaaaagagt aaagaattgg tcaaaaccaa tcaaaactcc cccaagtcca atcattacag
    25741 gtaaaactac tagagccagg agactttttc gataaagaga caaaaagtcc tttttcacaa
    25801 tcctattgac aaagacatag aaacttggca gtgtcactag agctactagc tgaaccaaat
    25861 gaaagagatt cttgaccact gcgaaatggt gcagaccagc tgctgacgaa cgaaaatcag
    25921 gcatctgtaa gacctgacta aaaggattgg tcagataatt catcaagata tgaaaattgt
    25981 attgaatggt ttctggtttt agatagactc gattcgttaa gtttagccac tgaatctcca
    26041 taggatagaa aatccaagcc agataaatgg tcagaaggat ggagagggag aggagaaaga
    26101 gcatagagcc ccaaaagatc aatttagttt tcatcaaaat cccactccgc aaggctagaa
    26161 accacatgtg tcggtgcgat tggcaggcca gctacttctt ctgccttagt aaaacctgtc
    26221 gtcaccaaga gcgttggaat gccattgtca atcccagccc gaatatcagt caaataattg
    26281 tccccaacca tgattaactc ttcacgttcc aaacctaagt gctcaaccgc cttgtccata
    26341 atgatggcat ttggttttcc gatataaacc ggcttcactc gtgtcgctac ttcaagcagc
    26401 gtaatcagtg agccagcacc tggcaaaaga ccgcgttccg tcgggatgtt gaggtcagga
    26461 ttggttccga taaaatgggc acccttttga atagcaagag ttgctgtggc aaatttttca
    26521 tagtcgactt gccaatccag accaactacc acgtaggcag gtttttcctt gtcttccaca
    26581 taaccagccg ccttgatggc ttccttgagt cctgcttctc cgacgacata gacggtcttt
    26641 tcaagcccca aatcattcat atagtcgatg gttgccaaag tcgctgtgta gacagtcgat
    26701 aggggcgtat cgatattaaa attctgagcc aacatctcct taacactctc tggagtgcgg
    26761 gttgtattgt tggttacaaa gagataggga atgtcccgct tttgcaattc atgaacaaaa
    26821 gtctctccag cagggattcg gtctttcccc ttataaatgg ttccgtctaa atcaattaaa
    26881 tagcctttat atttcatcta tttctcccta agcctttttt atttcttgcc aagtaatgat
    26941 tgcttgggca ttgataaccc catcacttgt aatttcatgc ttgctttcca gtccagtccg
    27001 ttcaacagcc gatgtaatca ccccacctgg tcgaacttcc ttgacatact tgaggttgat
    27061 tttcttggga atatagtggg tcaaaaaatc cgctcccatg acctcaaaaa tccagtccaa
    27121 gtatttactg ttattgacat gaccattcat atccaagtcg taaaaacgaa catggtaatc
    27181 cttgctgatc ggttcttcca aggactcata cttcggtcca cggataagtt ttttatcaaa
    27241 atcagactgg taaggagcca caatctcagg ttcaacaaca tggacttttc gactgtcgcg
    27301 gtccatgaga acaaaggtcg ccatcatgtg gatgagctcc tgctccgctt cattataaat
    27361 agtaaagcga cggtagcaaa aaagtcgatt gtagctcaag gcttccgttt cgatggtaat
    27421 ttcttccgca aaacgaggca aacgaaacac ctcaatatca tattctacga taatccagac
    27481 cagattatat tcttccaaaa tggccttatc actaactccc agttcaatcg actgcatccc
    27541 tgaaacttgc agtgacagca aaatcacatc tggaagtttg atatgaccgt tcatatcagc
    27601 catatcaaaa ggaattttca ttttcatttg ataagttaag cccatgatcc tactccaaaa
    27661 taaatcgttc tgctacagta tctcccaaaa agagacctct ctttgtcatg cgaacgtggt
    27721 caccctcaat ctgcatgagg ccttgttgaa ccaaatctct gacaatttct ccataaagtc
    27781 cagcaaaaga ctgtccaaat ttttcctcaa atcgcgccat ggaaaccccg gatttcttgc
    27841 ggagtcccaa gaacatttct tcttccattt gctccttttg actcaggtga tcttctgtaa
    27901 tacaagcatt gccttcctca accgcactga gataatgacg aatgggacca tgatttttat
    27961 agcgtactcc attgacataa ccagatgccc ctgcaccaat accatagtat tcagcattgt
    28021 cccagtacat gagattatga cgactttcaa agccgggttt ggagaaatta gaaatctcat
    28081 aatgctcaaa acccgctcgc tccagctctg caatgatgta ctcaaacatc tccgcttcta
    28141 gttcctcctt aggcagaggc aatttcccac gtcgcatccg gttcataaag accgtatggt
    28201 tttctaaaat caaactatac aaactcatgt ggggaatatc caatccaatg gctttagcca
    28261 cattttcctt tacttgctcc atggtctgac caggcagagc ataaatcaaa tcaatggaga
    28321 tgttgtcaaa accagccagt ttcaggcgat cgatattttc ataaatatcc ttctccaaat
    28381 gactgcgccc aatctttttc aacatcttat catcaaaggt ctggacacct agcgaaacac
    28441 gattgacagc cgaatttttc aaaacagcta tcttatccgc atccaaatcg cctggattgg
    28501 cttcaatggt caactcttcc aagacagaca aatccaagtt tttagtcaag ccattcagta
    28561 acacctccag ttgcggagcc gacagggctg tcggtgttcc accaccgata taaagggttg
    28621 acaacttttc aatatcataa gaacgaaact cttccagcag atgctctaaa tagctgtcga
    28681 ctggctgatt tttgatgaag acctttgaaa aatcacaata ataacaaatc tgggtacaaa
    28741 atgggatgtg cacataggct gacgttggtt ttttctgcat agtaattatt ataccacaaa
    28801 gactagattc cagataaaaa tcaccatccc cagatacata gtccgtccgg agatggtgat
    28861 ggtttattct tctgttatat caatcacaat ctcttctgag tcatcaagag cttcggcttt
    28921 ttcttgccat tgttccttga gattatttaa ttgatttttt gatgcttctg tcgcttgaaa
    28981 agcataggat ttagcttgag caagtatact gtccacagtg atttcacctg actcaacctg
    29041 ttcttttgtt ttcagaacaa aatctgtagc ctgctcctta acttctgtca gtttttcaca
    29101 gacttgctcc ttggcatact ccggatcttc tctcaaatca tctagaaaat cttgagcctg
    29161 actgcaaact tgtttgccct tatcacttgt taaaaacaag gcaagagctg cacctgaaac
    29221 ggttcctaaa aggattgagg ataatttacc cataaggatt ctcctttttt attttttgaa
    29281 aaatttactt gcaagacgaa gagctgacag acttgcacca gtcttgagtg tttttgaacc
    29341 agctgatgaa gctttcttgc tcaagacacg cgcatggtca ttgaggtctg aaacagatag
    29401 agataaatct gcaacagcac tgaagagtgg atcaatcgta gccaccttga cattgatatc
    29461 atctgccaag acattgacct tagccaacaa ctcattggtg tgatgcaagg tcacatccac
    29521 atctgaagtc aaggttttaa tcgtcttttc tgtttcatcg atgacacgac caagcttttg
    29581 tacagtaatg atcagataga ccaaaaagac aatcacagct agggcaacaa gaatatatgc
    29641 aacttctaac atttagtttt cctcctctgt aatatagtaa ggggccttct ttcgattttg
    29701 ataaataacg atcattatac cgagaccgat aaagacaact gacagccatt gggacactcg
    29761 aaggccgaag aacatgagac tatctgttcg cataccttcg atgaccatac gaccgaaacc
    29821 ataccaaatc aagtaaaagg ccgtgatatg acctcgtctg agactcttcc atttccgtct
    29881 aaaaatcaga atcaaggcaa agccaagcag attccataga gactcataaa ggaaagtcgg
    29941 ttgatggtag ctcccctcaa tatacatctg gtcacggata aagccaggta gataatccag
    30001 attatccact gttgcaccat aagcttcttg gttaaagaaa ttaccccaac gccccaaact
    30061 ttgagcaatc ataacgctag gcgccgcaat atctagaaaa tcccaagtat tgatgagttt
    30121 acggtcggca aagatataga gcacaagagc cccagttatc aaaccaccgt aaatggccaa
    30181 accaccattc caaatggcaa aaatctctcc taaattctga ctatagtaat caaatcggaa
    30241 aataacatag tagagacgag ctcctaaaat agccaaggga aaggctacta agataaaatc
    30301 taaaatatcg tctggtatga tcttctttct aggtgcttct ttcatggtca aataaaccgc
    30361 aagaatcaag cctgtcacaa tacataaggc ataccaacga atggctaggg gtcctagttg
    30421 aatagcaatt ggatcaagca ttttgcacct catttcgagc tatgagactt gtcagtcgtt
    30481 cgtcgaacaa acgggtagca tcaaagccca tttccttggc acgataattc atggcagctg
    30541 cctcaatcac aacagagata ttacgacctg ttttaactgg aatacgaata cgaggaatgg
    30601 ctacgccaga aacttcaagt tcctctgcat tgtttccaag acgatcaaag gtcttatgcg
    30661 tatcgtaatt ttccaaatag acagcaagct gaacctgtga agaatccttg acagcactcg
    30721 caccgtagag actcataaca tcgataatac caaccccacg aatttcaatc aagtgtttca
    30781 aaatttcagc tggttcaccc cagagagtaa tctcatcctt ggcaaagata tcgacacggt
    30841 catcggctac caaacggtga ccacgtttga caagctcaag acctgtctcg ctcttaccaa
    30901 ttccactatc tccctgaatc aagacgccca tcccataaat atccatcaag acaccgtgca
    30961 cactggtacg ttctgccaaa cgagaatcca gatagctaga taactctcca gataaacgac
    31021 tggtagctgc acggctggtt aaaatagcaa tcttacattc tctagcagcc tttaacatct
    31081 cctctggaac caccaaacca cgggcaacaa tgactgctgg tgtctcaggt agaaacattt
    31141 ttttcaaaac ttcataacgg ctgttggaag gcatgctgat cagataagac cactccttca
    31201 tccccaaaag ttgaatccgc tctggtgtat agtagtcaaa atagcctgtc atttcaagac
    31261 caggtcgcgt aatatccgct gtattgattt ccttttcaag caattctggt tcaccataga
    31321 caatatctag tctaagcttt tcaatcactt cttttactaa aaccgacatc atttcctcct
    31381 tcaaatgaat tcttctctct attatatcaa attgtaggag gaagtttcag atttttgcag
    31441 gctttggagt atttatttaa aagaaaaagg actagatttc tctagccttt catttcaatt
    31501 agaatttttt acgtttacga gctacttctg atttacgttt acgttttaca gaaggttttt
    31561 catagaattc acgtttgcgt gtttcttgaa gagtaccagc tttagtaacc gcacgtttga
    31621 aacggcgaag tgcgtcgtca agagattcat tcttacgtac tactgtttta gacatttttt
    31681 tcactccctt caagttcaat atctatttca ttctacacgt aaaaggaata attgtcaagc
    31741 aaaaatgcgg tttaattttg attttttctt ccatttatac aagagttgaa agcgttcaat
    31801 agaacattgc ttttttattt ttcgagtaag ctaagcgctt cagcatctgc gatgatggtt
    31861 acatcagggt gattttggag gctacttgca ggtaggttct cagtcactgg gccagatact
    31921 gttccggcaa tggcttctgc tttcgactca ccataagcaa aaagaataat agacttggca
    31981 tccaaaatgt ttttaatccc cattgaaata gcttgggttg ggacgtcttc aatcttggca
    32041 aagaagcgag cattggcttc gatagtagac tggtcaagtt ctactagatg cgtttgactg
    32101 tcaaatggag taccaggctc attaaagccg atatgcccat tgcgaccgat tcccaagatt
    32161 tggaggtcaa ctggatggtc agccaaaatt tggttgtagc gttcaacttc agcttcagca
    32221 ttgtccttaa caccacgagg caagaaactt tctttaaatg gtttttggtt gaacaagttt
    32281 tcttgcatga agtaacgata agactgtgga ttgtccccat caaggcccac atactcatca
    32341 aggttgacac tggttagatt tgaaaaatca aggtcactct caacaatttc cttgtaaaat
    32401 tcaagtgggc tacttcctgt cgcaagtcct agagtttgag ctccattagc caatttttcc
    32461 ttcaaaatct caaaagcaac ttttccacct tgaacttggt tttcaacttt aataactttc
    32521 atcttttcat cctccatttc tgtctatatt tattatatgg tatagaccaa tttttgtcaa
    32581 gtgaaaacga tttaatttct aaaaaaagag cgtccaaact tgaaacgtgt tataatggat
    32641 aggattagaa cttagaaaga atgaacaaat atatgaatac agctgatttt gatttccact
    32701 tgccagagga attgattgcc caaacgcccc ttgaaaaacg tgatgcctcc aaactcctca
    32761 tcgtcaaccg tgagacagga gaaatgcaag ataaacattt ccactctatt attgatatgc
    32821 tggaacctgg tgatgccctt gtcatgaacg acacccgagt tctccctgcc cgcctctatg
    32881 gtcaaaaagt ggagacagga ggtcatgtgg aacttctcct ccttaagaac actagtggag
    32941 acgagtggga agttctggct aaacctgcca aacgcctcaa ggtcggtact cgtatcagct
    33001 ttggtgatgg ccgcctcagc gctgtcgtta cagaagaatt gacccacggg ggacgcattg
    33061 tccgctttga ataccaagga attttcctag aagtcttgga aagtctggga gaaatgcctc
    33121 tgccacctta tatccacgaa aaattagatg accgtgaacg ttatcaaacc gtctacgcca
    33181 aggaaagcgg ctctgctgca gcaccgactg ctggtcttca cttcaccaaa gaactgctgg
    33241 cagaaatcca agctaagggt gttcatctag tctatctgac tctccatgtc ggactcggaa
    33301 cctttagacc tgtttctgtg gataatctgg acgaacacga aatgcactca gagttctatc
    33361 aactttctga ggaagctgct gccacccttc gctctgtcaa agaaaatggt ggtcgtgtca
    33421 tcgctgtcgg aaccacttct atccgtacct tggaaactat tggttccaag tttgatgggc
    33481 aaatccaagc agattctggt tggaccaata tctttatcaa acctgggtat gagtggaagg
    33541 tcgtggatgc cttctcaacc aacttccatc tgccaaaatc aactctggtc atgttggttt
    33601 ctgcctttgc aggccgtgaa ttagtcttag atgcctacca ccatgccatc caagaacact
    33661 accgcttctt cagttttggt gacgccatgt ttatttattg agaaagaatt tctctaaatc
    33721 ttctaatacc aataaatcgc taagatatta tttcaaagaa catctacaat tgaaactcta
    33781 gctagctgta gaagaggcct agtacattga aattaaaatg cttcccccta gcttcgaaaa
    33841 tattgccata gattgcgttg actctccaaa ttgattcatc tatattttat ttcagcttcc
    33901 tatattttct tcgctgtttg taaatcaaaa tgcaagacac atgagtagca ccatatttgt
    33961 tactcttatc tgtcctctca agagactatt atgagttatt tcagaatcat tcactacttt
    34021 gaccctgact ctccttagtc tcaaaatcaa agacttatac tcttcaaaaa tctcttcaaa
    34081 ccgcgtcaac gtcaccttgg attatatatg tgactgactt cgtcagttct atctacaacc
    34141 tcaaagcagt actttgagca acctgcgact agttttctag tttgctcttt gattttcatt
    34201 gagtattaaa caaaaagtga acaaatctga attctaatgt acagaagact aggcttgttc
    34261 acttttttat agtcgctata agatgacctt atctatggct ttttatatat aattatatat
    34321 tcagacatac tattatcaat tttgtcgcag ggaggaatct gttaacgcac ccattcacca
    34381 ttatcattaa ctctatagcc atctatactt gtattgaccg ctaactcacc cgatgcattt
    34441 acataatacc atttaccacc aacttggaac cattgattga ctttcataga accgttgctg
    34501 ttgagatagt accatgaacc attaacttgt acccaacctg ttgccatgga accatcagta
    34561 ttataaaaat accacatacc attttcttgt ttccagcctg ttgttggagc aactgcttta
    34621 gctggttcta ctgctacatc tgttccttgg ttagatgtaa cagatacagg atacgaagga
    34681 atagatgatt gctcaggaac aacaactttt tcaggttctc tcgtccctct ccttatacgt
    34741 ctttttacca tctctttagt aatttgacga gaagtagttt cttcaattgt tccatcacgt
    34801 tcatctacag tatagattgt agtaagagta atttaccaat ttctcctact tcttctactt
    34861 cttgactttt atcaagagtt gggccatcga gatattctgt ttcgattgga atttcttgga
    34921 caagaacttg gggcttggtt ccttttttaa caactcttgt ttgagagtct tctttttgac
    34981 ttaaagtact ctcagttact tgtccactct ttccatctac atcataagtt atcgttgtaa
    35041 ctgttttccc attctttcct agagtaatct cttgctcctg tcctgcagaa aggtcattgt
    35101 ctgcttcata tttagtagca aatggaacaa aaacttcttc aaccttgctt ttagctggaa
    35161 ctttgataac tgtatccgtg gcttcttttc tatcaacagt aacctgttcg gtaacatgac
    35221 cagtctctgg attaacatcg taggtccttg tcgtagttac atagccatcc tctccatcaa
    35281 ttgtaacagg attttcacta cggtcttttg tttcatcttt ttcataacga attcgcgtac
    35341 ttgaaatttt cttggttact accttaggtt tagtcgctac ttttacaata atatccccat
    35401 tgtcagcgtc atcatactct attccctctt ctttatctct agtatcatct ctgacatatt
    35461 gaatcccatc agcagcatga acaaaacttg tattcagatt cctcctaaaa ataaagttag
    35521 cccgattacc gcagaaccaa aaatctttcc gagtttacgt attgcatagc gcttattagt
    35581 attagatttt gccattacat cctacttcta gtatagcatc ttttctatca aacgttaaac
    35641 aatatacttt atatataaaa tagacttaga atgatatatt gatt
//
LOCUS       DAVLVP010000012        34676 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.012, whole genome shotgun sequence.
ACCESSION   DAVLVP010000012 DAVLVP010000000
VERSION     DAVLVP010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.012"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            147..1493
                     /gene="celB"
                     /locus_tag="VYN69_000574"
     CDS             147..1493
                     /gene="celB"
                     /locus_tag="VYN69_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEV1532891.1"
                     /translation="MDQQNGLFGFLENHVMGPMGKLAQFKVVRAITVAGMAAVPFTIV
                     GSMFLVFSILPQAFSFWPIVADIFSASFDKFTSLYMVANYATMGSLSLYFVLSLAYEL
                     TKIYAEEEELNMNPLNGALLALMAFVMTVPQIIFDGGMMKTVTSLKEGAVIADGWAMG
                     NGVARFGTTGIFTAIIMAIVTVLIYRMCVKHNWVIKMPEAVPEGVSRGFTALVPGFVV
                     AFVVIFINGLLVAMGTDIFKVIAIPFGFVSNLTNSWIGLMIIYLLTQLLWIVGIHGAN
                     IVFAFVSPIALANMAENAAGGHFAVAGEFSNMFVIAGGSGATLGLCLYIAFASKSEQL
                     KAIGRASVVPALFNINEPLIFGLPIIYNPALAIPFILAPMVTATIYYVANSLNFIKPI
                     IAQVPWPTPVGIGAFLGTADLRAVLVALVCAFAAFLVYLPFIRVYDQKLVKEEQGI"
     gene            1873..2049
                     /locus_tag="VYN69_000575"
     CDS             1873..2049
                     /locus_tag="VYN69_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532892.1"
                     /translation="MSLDIDKEKMTIMGIAFENRSVFKSVWYALSTNMIEGWRPTVSD
                     VEKLRDEALSLGMT"
     gene            2451..4664
                     /locus_tag="VYN69_000576"
     CDS             2451..4664
                     /locus_tag="VYN69_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HEV1532893.1"
                     /translation="MKIFKGEFYRISVLTDKLVRLEYSRTGNFEDRTTQLIYNRDFGQ
                     VSLDYIETSNVLDIMTDYFHLHFNKGEFNAENLFIELKGNFAVYDSRWYFGESIETLK
                     GTARTLDKADGAISLEDGIISRNGIALLDDSQGFIWDEQSGYIERENQIDLYFFAYGH
                     DYRGAIRDFYHLTGSTPLLPRYALGNWWSRYWPYTSDEYLDLIDRFETEKIPLSIGVL
                     DMDWHITDIPARFGSGWTGYSWNRNLIPNPEQLLQQLHDRKLKLSLNVHPADGIRAYE
                     EAYPQIAKRLGLNVELEEPAIFDFFNPSFREAYFKDVHYELEKQGVDFWWIDWQQGTQ
                     GMLDPLWLLNHYHYQDSCKNAEGGLILSRYAGPGSHRYPVGFSGDTIISWNSLRFQPY
                     FTATASNIGYSWWSHDIGGHMLGDYDEELQTRWLQFGVFSPITRLHSSRSPFNSKEPW
                     FFSETTSKIMKKYLRLRHQMIPYLYTMNVKTHEEGAPLISPIYYFYPENDESYNVPNQ
                     YFFGTELMVAPIVEKMDLTFQSAKVDVWFPEGEWYDFFSEKKYTGGVKLSVYRDISTT
                     PVFAKSGAIIPLVGSEIGMGVDLPEVVDWYVFPGKQHSFEMLEDQNGQRYKTRLSIDW
                     EMGMVELALQGDSSIVPSNRKHRIHFKGTNVSIIELPNKNDTAKFEWKDNKRTSLNDE
                     VFRLLKTASLPYELKDRLLNQFINAKNSHDLMNILHHQDKELRGRLLEMIFTSQN"
     gene            complement(4886..5362)
                     /locus_tag="VYN69_000577"
     CDS             complement(4886..5362)
                     /locus_tag="VYN69_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEV1532894.1"
                     /translation="MTSLYDFSVLNQNNQATPLDSYRGKVLLIVNTATGCGLTPQYQG
                     LQELYERYQDQGFEILDFPCNQFMGQAPGSAEEINAFCSLHFQTTFPRFAKIKVNGKE
                     ADPLYVWLKDQKSGPLGKRVEWNFAKFLIGRDGQVFERFSSKTDPKQIEEAIQTLL"
     gene            5585..8788
                     /locus_tag="VYN69_000578"
     CDS             5585..8788
                     /locus_tag="VYN69_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001193686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored hyaluronate lyase"
                     /protein_id="HEV1532895.1"
                     /translation="MQTKTKKLIVSLSSLVLSGFLLNHYMTVGAEETTTNTIQQSQKE
                     VQYQQRDTKNLVENGDFGQTEDGSSSWTGSKAQGWSAWVDQKNSSADASTRVIEAKDG
                     AITISSHEKLRAALHRMVPIEVKKKYKLRFKIKTDNKIGIAKVRIIEESGKDKRLWNS
                     ATTSGTKDWQTIEADYSPTLDVDKIKLELFYETGTGTVSFKDIELVEVAAQLSEDSQT
                     DKQLEEKIDLPIGKKHVFSLADYTYKVENPDVASVKNGILEPLKEGTTNVIVSKDGKE
                     VKKIPLKILASVKDTYTDRLDDWNGIIAGNQYYDSKNEQMAKLNQELEGKVADSLSSI
                     SSQADRTYLWEKFSNYKTSANLTATYRKLEEMAKQVTNPSSRYYQDETVVRTVRDSME
                     WMHKHVYNSEKSIVGNWWDYEIGTPRAINNTLSLMKEYFSDEEIKKYTDVIEKFVPDP
                     EHFRKTTDNPFKALGGNLVDMGRVKVIAGLLRKDDQEISSTIRSIEQVFKLVDQGEGF
                     YQDGSYIDHTNVAYTGAYGNVLIDGLSQLLPVIQKTKSPIDKDKMQTMYHWIDKSFAP
                     LLVNGELMDMSRGRSISRANSEGHVAAVEVLRGIHRIADMSEGETKQRLQSLVKTIVQ
                     SDSYYDVFKNLKTYKDISLMQSLLSDAGVASVPRTSYLSAFNKMDKTAMYNAEKGFGF
                     GLSLFSSRTLNYEHMNKENKRGWYTSDGMFYLYNGDLSHYSDGYWPTVNPYKMPGTTE
                     TDAKRADSDTGKVLPSAFVGTSKLDDANATATMDFTNWNQTLTAHKSWFMLKDKIAFL
                     GSNIQNTSTDTAATTIDQRKLESSNPYKVYVNDKEASLTEQEKDYPETQSVFLESSDS
                     KKNIGYFFFKKSSISMSKALQKGAWKDINEGQSDKEVENEFLTISQAHKQNGDSYGYM
                     LIPNVDRATFNQMIKELESSLIENNETLQSVYDAKQGVWGIVKYDDSVSTISNQFQVL
                     KRGVYTIRKEGDEYKIAYYNPETQESAPDQEVFKKLEQAAQPQVQNSKEKEKSEEEKN
                     HSDQKNLPQTGEGQSILASLGFLLLGAFYLFRRGKNN"
     gene            9172..9557
                     /gene="tnpA"
                     /locus_tag="VYN69_000579"
                     /pseudo
     CDS             9172..9557
                     /gene="tnpA"
                     /locus_tag="VYN69_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005816675.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            complement(9936..10565)
                     /locus_tag="VYN69_000580"
     CDS             complement(9936..10565)
                     /locus_tag="VYN69_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEV1532896.1"
                     /translation="MTKSDTIIELKKQKIVAVIRGNTKEEGLQASIACIKGGIKAIEI
                     AYTNQYAGQIIKELVDLYQDDQSVCIGAGTVLDAVTARDAILAGANYVVSPSFHAETA
                     KMCNLYSTPYIPGCITLTEITTALEAGSEIIKLFPGSTLSPAYISAVKAPIPQVSVMV
                     TGGVGLNNIPQWFAAGADAVGIGGELNKLASQGNFDRISEIAQQYITLR"
     gene            complement(10575..11576)
                     /locus_tag="VYN69_000581"
     CDS             complement(10575..11576)
                     /locus_tag="VYN69_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEV1532897.1"
                     /translation="MTKILLFGEPLIRISPLDATSIGDHVASSTYFGGSEINIACNLQ
                     ALGISTKVFTALPANEIGDRFLTFLKQHQIDTSSICRLGDRIGLYYLENGFGCRQSEV
                     FYDRKHTSISQIRPNMLDMDSLFQGISHFHFSGITVAIGQEVRAILLLLLEEAKRRGI
                     VVSMDLNLRTKMISVLEAKYEFSKFARFTDYCFGIDPLMIDDQNLEMFPRDSASLEEV
                     ENRMRLLKDAYGFKAIFHTLRSSDEQDKNVYQAYALEERFEESVQLKTAVYQRIGSGD
                     AFISGALYQLLHHSSLKTTIDFAVASATLKCTLPGDHLSTSATSIENLLANAQDIIR"
     gene            complement(11607..12248)
                     /locus_tag="VYN69_000582"
     CDS             complement(11607..12248)
                     /locus_tag="VYN69_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HEV1532898.1"
                     /translation="MKIALINENSQASKNHIIYDSLKEATDKKGYQLFNYGMRGEEGE
                     SQLTYVQNGLMAAILLNTKAVDFVVTGCGTGVGAMLALNSFPGVVCGLAVDPTDAYLY
                     SQINGGNALSIPYAKGFGWGAELTLKLMFERLFAEEMGGGYPRERVIPEQRNARILNE
                     VKQITHNDLMTILKIIDQDFLKDTISGKYFQEYFFENCQDDEVAAYLKEVLAK"
     gene            complement(12267..13082)
                     /locus_tag="VYN69_000583"
     CDS             complement(12267..13082)
                     /locus_tag="VYN69_000583"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEV1532899.1"
                     /translation="MTNTSFSIEQFSLKGKIALITGASYGIGFAIAKSYAEAGATIVF
                     NDINQDLVNKGIEAYREVGIQAHGYVCDVTDEDGIQAMVKQIEQEVGVIDILVNNAGI
                     IRRVPMCEMSAADFRKVIDIDLNAPFIVSKAVIPSMIKKGHGKIINICSMMSELGRET
                     VSAYAAAKGGLKMLTRNIASEYGGANIQCNGIGPGYIATPQTAPLRELQEDGSRHPFD
                     QFIIAKTPAARWGNTEDLMGPAVFLASDASNFVNGHILYVDGGILAYIGKQPE"
     gene            13353..13787
                     /locus_tag="VYN69_000584"
     CDS             13353..13787
                     /locus_tag="VYN69_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1532900.1"
                     /translation="MKIVLVGHGHFATGIYSSLQLIAGNQENVEAIDFVEGMSADELK
                     QKILLAISNEEEVLILSDLLGGSPFKVSSTIMGENPAKTMNVLSGLNLAMLMEAVFAR
                     MAHSFDEVVNKSVVAAQGGVVNGKELFSTDAEEEEEDFESGI"
     gene            13799..14989
                     /locus_tag="VYN69_000585"
     CDS             13799..14989
                     /locus_tag="VYN69_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003074342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="unsaturated chondroitin disaccharide hydrolase"
                     /protein_id="HEV1532901.1"
                     /translation="MIKKVTIEKIKSPERFLEVPLLTKEEVGQAIDKVIRQLELNLDY
                     FKEDFPTPATFDNVYPIMDNTEWTNGFWTGELWLAYEYSQQDAFKNIAHKNVLSFLDR
                     VNKRVELDHHDLGFLYTPSCMAEYKINGDGEAREATLKAADKLIERYQEKGGFIQAWG
                     DLGKKEHYRLIIDCLLNIQLLFFAYQETGDQKYYDIAESHFYASANNVIRDDASSFHT
                     FYFDPETGQPFKGVTRQGYSDDSCWARGQSWGVYGIPLTYRHLKDESCFDLFKGVTNY
                     FLNRLPKDHVSYWDLIFNDGSDQSRDSSATAIAVCGIHEMLKHLPEVDADKDIYKHAM
                     HAMLRSLIEHYANDQFTPGGTSLLHGVYSWHSGKGVDEGNIWGDYYYLEALIRFYKDW
                     NLYW"
     gene            15000..15491
                     /locus_tag="VYN69_000586"
     CDS             15000..15491
                     /locus_tag="VYN69_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1532902.1"
                     /translation="MTMPNIIMTRIDERLIHGQGQLWVKYLGCNTVIVANDEVSTDKM
                     QQTLMKTVVPDSVAMRFFPLQKVIDIIHKANPAQTIFIVVKDVKDALTLVEGGVTIKE
                     INIGNIHNAPGKEQVTRSIFLGEEDKAALKELSQTHQVTFNTKTTPTGNDGAVQVNIM
                     DYI"
     gene            15506..16285
                     /locus_tag="VYN69_000587"
     CDS             15506..16285
                     /locus_tag="VYN69_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1532903.1"
                     /translation="MSINVFQAILIGLWTAFCFSGMLLGIYTNRCIVLSFGVGIILGD
                     LPTALAMGAIGELAYMGFGVGAGGTVPPNPIGPGIFGTLMAITSAGKVSPEAALALST
                     PIAVAIQFLQTFAYTVRAGAPETAMKHLKNHNLKKFKFTLNATIWLFAFIGFTLGCLG
                     ALSMDTLLKLVDYIPPVLLTGLTVAGKMLPAIGFAMILSVMAKKELIPFVLLGYVCAA
                     YLNIPTIGIAIVGTIFALIEFYNKPKTADHVVEEEAHDDWI"
     gene            16272..17090
                     /locus_tag="VYN69_000588"
     CDS             16272..17090
                     /locus_tag="VYN69_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1532904.1"
                     /translation="MTGSNKLTKRDYLKTSLRAFFCQNGFNYSNYQGLGYANVMYPAL
                     KKHYGEDQEGFYQALEENCEFYNTNPHFLPFITSLHLVMLENGRPAKETRSIKMALMG
                     PLAGIGDSLSQFCLAPLFSTIAASFAQEGLVVGPILFFLAMNTILTAIKLSTGLYGYK
                     LGTTVIDKLSEQMATISRIANIIGVTVIAGLAATSVKIMVPITFAAGEVKADAKQSIV
                     SIQGMLDKVAPALLPALFTLLVYYLIKEKKWTTYKLVILTVIIGIIGSWLKIIA"
     gene            17090..17383
                     /locus_tag="VYN69_000589"
     CDS             17090..17383
                     /locus_tag="VYN69_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000381741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1532905.1"
                     /translation="MDTTLFYGIVIVLAVSPLLLSSFHSIRQQKLLRKQMEQRQEYLA
                     SLTSGDEVLLLSGIHGKIISIKDDLVSLQIAKGVVIYVEKESVMGKTKELLFK"
     gene            17405..19306
                     /locus_tag="VYN69_000590"
                     /pseudo
     CDS             17405..19306
                     /locus_tag="VYN69_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000657070.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alginate lyase family protein"
     gene            19366..20367
                     /locus_tag="VYN69_000591"
     CDS             19366..20367
                     /locus_tag="VYN69_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1532906.1"
                     /translation="MEKKLTIKDIAEMAQTSKTTVSFYLNGKYEKMSQETREKIEKVI
                     HETNYKPSIVARSLNSKRTKLIGVLIGDITNSFSNQIVKGIEDIASQNGYQVMIGNSN
                     YSQESEDRYIESMLLLGVDGFIIQPTSNFRKYSRIIDEKKKKMVFFDSQLYEHRTSWV
                     KTNNYDAVYDMTQSCIEKGYEHFLLITADTSRLSTRIERASGFVDALTDANMRHASLT
                     IEDKHTNLEQIKEFLQKEIDPDEKTLVFIPNCWALPLVFTVIKELNYNLPQVGLIGFD
                     NTEWTCFSSPSVSTLVQPSFEEGQQATKILIDQIEGRNQEERQQVLDCSVNWKESTF"
     gene            complement(20477..20812)
                     /locus_tag="VYN69_000592"
     CDS             complement(20477..20812)
                     /locus_tag="VYN69_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000838135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532907.1"
                     /translation="MKSLARLLNIHVFISIFLFFALISGAVSHTVLLLLLLFLPALNK
                     GLEKIQSKRIPVLNAALFFLLISFPQLLTNPVQWKFSIFLVVTIISSLAYFYNFYQVV
                     KEVDQKQLI"
     gene            complement(20823..21065)
                     /locus_tag="VYN69_000593"
     CDS             complement(20823..21065)
                     /locus_tag="VYN69_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532908.1"
                     /translation="MKKEKKQLRYPGLKAGSIYGTVIFFIIPLIDTLTSENPNFISSL
                     LNTKHIFKTILGAFFFGVMIHIVDSLRIARAKKDQD"
     gene            complement(21076..21618)
                     /locus_tag="VYN69_000594"
     CDS             complement(21076..21618)
                     /locus_tag="VYN69_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3278 domain-containing protein"
                     /protein_id="HEV1532909.1"
                     /translation="MKKETFTEKLIKRTYGISGPLDEYKRREADSIGNQVFIVLFYLM
                     IFGNLIPLLLAYKYPQEVALIYPPLILVIALIAAGYVTYQMKKTGITVIEPDMLNEKE
                     SKQLHYPGLKAGLFFGLWMFFITPLLSILIDEGQDYFHSLLTIRNGVSSILGSIFFGA
                     SIQFLISRRIAKTKKNQDED"
     gene            complement(21634..21828)
                     /locus_tag="VYN69_000595"
     CDS             complement(21634..21828)
                     /locus_tag="VYN69_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001082472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1532910.1"
                     /translation="MNRVKEFRKELGISQLELAKDIGVSRQTINMIENDKYNPTLELC
                     LNLARSLQTDLNSLFWEEDF"
     gene            21994..22944
                     /gene="rsmH"
                     /locus_tag="VYN69_000596"
     CDS             21994..22944
                     /gene="rsmH"
                     /locus_tag="VYN69_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEV1532911.1"
                     /translation="MTKEFHHVTVLLHETIDMLDVKPDGIYVDATLGGAGHSEYLLSK
                     LSEKGHLYAFDQDQNAIDNAQKRLAPYIEKGMVTFIKDNFRHLQARLREAGVQEIDGI
                     CYDLGVSSPQLDQRERGFSYKKDAPLDMRMNQDASLTAYEVVNNYDYHDLVRIFFKYG
                     EDKFSKKIARKIEQAREVKPIETTTELAEIIKLVKPAKELKKKGHPAKQIFQAIRIEV
                     NDELGAADESIQQAMDMLALDGRISVITFHSLEDRLTKQLFKEASTVEVPKGLPFIPD
                     DLKPKMELVSRKPILPSAEELEANNRSHSAKLRVARKIHK"
     gene            22956..23273
                     /gene="ftsL"
                     /locus_tag="VYN69_000597"
     CDS             22956..23273
                     /gene="ftsL"
                     /locus_tag="VYN69_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEV1532912.1"
                     /translation="MAEKMEKTGQILQMQLKRFSRVEKAFYFSIAVTTLIVAISIIFM
                     QTKLLQVQNDLTKINAQIEEKKTELDDAKQEVNELLRAERLKEIANSHDLQLNNENIR
                     IAE"
     gene            23277..25529
                     /gene="pbpX"
                     /locus_tag="VYN69_000598"
     CDS             23277..25529
                     /gene="pbpX"
                     /locus_tag="VYN69_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2X"
                     /protein_id="HEV1532913.1"
                     /translation="MKWTKRVIRYATKNRKSPAENRRRVGKSLSLLSVFVFAIFLVNF
                     AVIIGTGTRFGTDLAKEAKKVHQTTRTVPAKRGTIYDRNGVPIAEDATSYNVYAVIDE
                     NYKSATGKILYVEKTQFNKVAEVFHKYLDMEESYVREQLSQPNLKQVSFGAKGNGITY
                     ANMMSIKKELEAAEVKGIDFTTSPNRSYPNGQFASSFIGLAQLHENEDGSKSLLGTSG
                     MESSLNSILAGTDGIITYEKDRLGNIVPGTEQVSQRTMDGKDVYTTISSPLQSFMETQ
                     MDAFQEKVKGKYMTATLVSAKTGEILATTQRPTFDADTKEGITEDFVWRDILYQSNYE
                     PGSTMKVMMLAAAIDNNTFPGGEVFNSSELKIADATIRDWDVNEGLTGGRMMTFSQGF
                     AHSSNVGMTLLEQKMGDATWLDYLNRFKFGVPTRFGLTDEYAGQLPADNIVNIAQSSF
                     GQGISVTQTQMIRAFTAIANDGVMLEPKFISAIYDPNDQTARKSQKEIVGNPVSKDAA
                     SLTRTNMVLVGTDPVYGTMYNHSTGKPTVTVPGQNVALKSGTAQIADEKNGGYLVGVT
                     DYIFSAVSMSPAENPDFILYVTVQQPEHYSGIQLGEFANPILERASAMKDSLNLQTTA
                     KALEQVSQQSPYPMPSVKDISPGDLAEELRRNLVQPIVVGTGTKIKNSSAEEGKNLAP
                     NQQVLILSDKAEEVPDMYGWTKETAETLAKWLNIELEFQGSGSTVQKQDVRANTAIKD
                     IKKITLTLGD"
     gene            25531..26511
                     /locus_tag="VYN69_000599"
     CDS             25531..26511
                     /locus_tag="VYN69_000599"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEV1532914.1"
                     /translation="MFISISAGIVTFLLTLVEIPAFIQFYRKAQITGQQMHEDVKQHQ
                     AKAGTPTMGGLVFLITSVLVAFFFALFSSQFSNNVGMILFILVLYGLVGFLDDFLKVF
                     RKINEGLNPKQKLALQLLGGVIFYLFYERGGDILSVFGYPVHLGFFYIFFALFWLVGF
                     SNAVNLTDGVDGLASISVVISLSAYGVIAYVQGQMDILLVILAMIGGLLGFFIFNHKP
                     AKVFMGDVGSLALGGMLAAISMALHQEWTLLIIGIVYVFETTSVMMQVSYFKLTGGKR
                     IFRMTPVHHHFELGGLSGKGNPWSEWKVDFFFWGVGLLASLLTLAILYLM"
     gene            27120..29225
                     /locus_tag="VYN69_000600"
     CDS             27120..29225
                     /locus_tag="VYN69_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001063457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1532915.1"
                     /translation="MNNNFNNFNNMDDLFNQLMGGMRGYSSENRRYLINGREVTPEEF
                     AHYRATGQLPGNAETDVQMPQQASGMKQDGVLAKLGRNLTAEAREGKLDPVIGRNKEI
                     QETSEILSRRTKNNPVLVGDAGVGKTAVVEGLAQAIVNGDVPAAIKNKEIISIDISGL
                     EAGTQYRGSFEENVQNLVNEVKEAGNIILFFDEIHQILGAGSTGGDSGSKGLADILKP
                     ALSRGELTVIGATTQDEYRNTILKNAALARRFNEVKVNAPSAENTFKILQGIRDLYQQ
                     HHNVILPDEVLKAAVDYSVQYIPQRSLPDKAIDLVDVTAAHLAAQHPVTDVHAVEREI
                     ETEKDKQEKAVEAEDFEAALNYKTRIAELEKKIENHTEDMKVTASVNDVAESVERMTG
                     IPVSQMGASDIERLKDMAHRLQDKVIGQDKAVEAVARAIRRNRAGFDEGNRPIGSFLF
                     VGSTGVGKTELAKQLALDMFGTQDAIIRLDMSEYSDRTAVSKLIGTTAGYVGYDDNSN
                     TLTERVRRNPYSIILLDEIEKADPQVITLLLQVLDDGRLTDGQGNTVNFKNTVIIATS
                     NAGFGYEANLTEDADKPELMDRLKPFFRPEFLNRFNAVIEFSQLTKEDLSKIVDLMLA
                     EVNQTLAKKDIDLVVSQAAKDYITEEGYDEVMGVRPLRRVVEQEIRDKVTDFHLDHLD
                     AKHLEADMKDGVLVIREKA"
     gene            29676..30011
                     /locus_tag="VYN69_000601"
                     /pseudo
     CDS             29676..30011
                     /locus_tag="VYN69_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(30078..30560)
                     /locus_tag="VYN69_000602"
     CDS             complement(30078..30560)
                     /locus_tag="VYN69_000602"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEV1532916.1"
                     /translation="MSKEVIVESFELDHTIVKAPYVRLIGEETGPKGDIISNYDIRLV
                     QPNEDSIPTAGLHTIEHLLAKLIRTRIDGMIDCSPFGCRTGFHMIMWGRHTSAKIAAV
                     IKDSLKEIAETTTWEDVPGTTIESCGNYKDHSLFSAKEWARLILEQGISDDAFERHVI
                     "
     gene            complement(30655..32139)
                     /locus_tag="VYN69_000603"
     CDS             complement(30655..32139)
                     /locus_tag="VYN69_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008535024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEV1532917.1"
                     /translation="MKKQAFSSEQYLNLQRDHILERINQFDGKLYLEFGGKMLEDFHA
                     ARVLPGYEPDNKIKLLQELKEQIEVVIAINASNIEHSKARGDLGISYDQEVLRLIDKF
                     NELGIFVGSVVITQYAGQPAADAFRNQLEKNGIDSYLHYPIKGYPTDMDHIISSKGMG
                     KNDYIKTSRNLIVVTAPGPGSGKLATCMSNMYHDQINGIKSGYAKFETFPVWNLPLHH
                     PVNLAYEAATADLDDVNMIDPFHLQTYGETTVNYNRDIEIFPVLKRMLERILGKSPYA
                     SPTDMGVNMVGFAITDDEAAVEASKQEIIRRYYQTVLDFKAEKVSEAAVKKIELLMND
                     LGITPADRKVAVVARQKAEETGGPALAFELPNGEIVTGKNSELFGPTAAALINAIKKS
                     ADIAKEVKLIEPEVVKPIQGLKIDHLGSRNPRLHSNEILIALAITATENPDAARAMEE
                     LGNLKGSEAHSTIILTDEDKNVLRKLGINVTFDPYYQYDRLYRK"
     gene            32292..33899
                     /locus_tag="VYN69_000604"
     CDS             32292..33899
                     /locus_tag="VYN69_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEV1532918.1"
                     /translation="MQEKWWHNAVVYQVYPKSFMDSNEDGIGDLPGITSKLDYLAKLG
                     ITAIWLSPVYDSPMDDNGYDIADYQAIAAIFGTMEDMDQLIAEAKKRDIRIIMDLVVN
                     HTSDEHAWFVEACENTDSPERDYYIWRDEPNDLDSIFSGSAWEYDEKSGQYYLHFFSK
                     KQPDLNWENEKLRQKIYEMMNFWIDKGIGGFRMDVIDMIGKIPDEKVVNNGPMLHPYL
                     KEMNQATFGDKDLLTVGETWGATPEIAKLYSDPKGQELSMVFQFEHIGLQYQEGQPKW
                     HYQKELNIAKLKEIFNKWQTELGVEDGWNSLFWNNHDLPRIVSIWGNDQEYREKSAKA
                     FAILLHLMRGTPYIYQGEEIGMTNYPFETLDQVEDIESLNYAREALEKGVPIEEIMDS
                     IRVIGRDNARTPMQWDESKNAGFSTGQPWLAVNPNYEMINVQEALANPDSIFYTYQKL
                     VQIRKENSWLVRADFELLDTADKVFAYIRKDGDRRFLVVANLSNEEQDLTVEGKVKSV
                     LIENTAAKEVLEKQVLAPWDAFCVELL"
     gene            34059..34232
                     /locus_tag="VYN69_000605"
     CDS             34059..34232
                     /locus_tag="VYN69_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001022221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteinase"
                     /protein_id="HEV1532919.1"
                     /translation="MNIKKRVLSAGLTFASALLLAACGQSGSDTKTYSSTFGGNPTTF
                     NYLLDYYTDNIVN"
     gene            complement(34225..34676)
                     /locus_tag="VYN69_000606"
                     /pseudo
     CDS             complement(34225..34676)
                     /locus_tag="VYN69_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT        10638 a         6101 c         7670 g        10267 t
ORIGIN      
        1 ttataaacga acagataagg cttatcaaca atttgtccaa ttggaaaaga actcacttag
       61 tttataatcc tgaactagta taatttgtat ttgaaactag tctttaaaaa gtaaaaatta
      121 ttatgtttta ttaaggaggt ctatttatgg atcaacaaaa cgggttgttt ggttttcttg
      181 aaaaccatgt tatgggacca atgggcaaac ttgctcagtt taaagtagta cgtgctatca
      241 cggttgcagg tatggctgct gtaccattta ctattgtagg atcaatgttt ttggtattca
      301 gtattttgcc acaagctttc tcattttggc caattgtggc agatattttc tctgcttcat
      361 ttgataaatt cacatcactt tacatggttg caaactatgc gactatgggt tctctatctc
      421 tttatttcgt tctatcactt gcatatgaat tgacaaaaat ttatgcagag gaagaagaac
      481 tcaatatgaa tcctcttaat ggtgccttgc ttgccttgat ggcttttgtc atgacagtac
      541 cgcaaatcat ttttgatggt ggaatgatga agactgtgac aagtctaaaa gaaggtgcag
      601 taattgcaga tggatgggca atgggaaatg gagtcgcacg ttttgggaca acagggattt
      661 ttaccgcaat cattatggca attgtgactg ttcttattta tcgtatgtgt gttaaacata
      721 attgggttat taaaatgcct gaagctgttc cagaaggagt ttctcgtgga tttaccgctt
      781 tggttccggg atttgttgtt gcatttgttg ttatctttat caacggtctt cttgtagcaa
      841 tgggaacaga tatttttaaa gtcattgcaa ttccatttgg ttttgtatcc aatctgacta
      901 attcgtggat tggtttaatg attatttatc tattgactca actactttgg attgtaggta
      961 tccacggtgc gaacattgtt tttgcatttg ttagtccaat tgctcttgct aacatggctg
     1021 aaaatgctgc tggcgggcac ttcgctgttg caggtgaatt ttctaatatg tttgtaattg
     1081 caggtggttc tggtgcaact ttaggactat gtttatatat tgcttttgcc tctaaatctg
     1141 aacagcttaa agcaatagga cgagcatctg tagttccagc cttatttaat attaatgaac
     1201 cattaatttt tggattacct attatctata atccagcctt ggctatacca tttattttag
     1261 caccaatggt tactgctact atttattacg tagcgaattc tctaaacttt attaagccaa
     1321 ttatcgcaca ggttccatgg ccaactccag tagggattgg agctttctta gggacagcag
     1381 atcttcgagc tgtattagtt gctctagtat gtgcatttgc agcattctta gtctatcttc
     1441 cattcatccg tgtatatgat caaaaattgg tgaaagaaga gcaaggtatc taataagtaa
     1501 aaaagtatat tcgatgatag aatccatata gtttaaataa tgagtgttaa tattagcagt
     1561 gaagaggtct ttttaagata ttcttctctg ctttttcata ttttttcgct atgtagggta
     1621 tgaagcaaaa tagaaagtag ttcgaatagt tgataaacag gaaactgctg ttatttaaaa
     1681 aaaacaataa agtaaagtat gttatcgtag aatacgatac aatagtaaaa aagcaaaaag
     1741 aatgcgtttt atatttgggt ttgaggctta taaaatagga taagcgtgct gaaaacttag
     1801 atatgacaaa caaaataaaa tttttaaaag ctacagagaa acatggtata atgtatctaa
     1861 aggaggtagc atatgtcctt agatatagat aaagaaaaga tgacaatcat gggaatagct
     1921 tttgaaaacc gatccgtttt taagagtgtt tggtatgctt taagtacaaa tatgattgaa
     1981 ggatggcgac ctacggttag tgatgttgaa aaattacgag atgaagctct ttctctgggg
     2041 atgacttaat gaagcgcaac tgttatgagt cttatgacta tgttgatccg gataatttat
     2101 ataccgatcc gggttcttca gttctaagaa ataagcagga aggaaaaaag cacgcgagct
     2161 tgaatatcgt atgattgcta gtaagagttt gaaattattt atcaatccta tcttagttaa
     2221 tagtttaaaa gtagtttgtt tcacaactgc ataagcttag aaaagttgaa aagtgataac
     2281 tctttaataa actcttggtt tgttaaaaga tattttgtcc actacatttt tttcaattga
     2341 ctattatttg tcatagatat aacagataga acattgcatt cagaataatg aaaaacgcat
     2401 caaaaatggt gcgttctgtt tttatgctac cataaaagga ggaaaacaaa ttgaaaattt
     2461 ttaagggaga gttttatcga atctctgtat taacagacaa gctagtaagg ttagaatact
     2521 ctcgaactgg aaattttgag gatagaacga cacaacttat ctataataga gattttggcc
     2581 aagtttcgtt agattatatc gagacatcaa atgtactaga tattatgacg gactattttc
     2641 atctgcactt taataaagga gaatttaacg ccgaaaattt atttatagaa ttaaaaggaa
     2701 attttgccgt atatgatagt cgctggtatt ttggtgaatc tattgaaacg ttaaaaggaa
     2761 cagctcggac tctggataag gcagatggag caatctcgtt agaagatgga attattagcc
     2821 gaaatggtat agccttattg gatgattctc aaggatttat ttgggatgaa caatctggtt
     2881 atattgagag agaaaatcaa attgacctgt atttctttgc ctatgggcat gattatagag
     2941 gagcaatcag agacttttac catttgactg gttcaacacc cttgttgcca agatatgctt
     3001 taggtaattg gtggagtaga tattggcctt atacgtcgga tgaatacttg gatttaatag
     3061 acagatttga aacagagaaa attccattat ctatcggtgt gttagatatg gattggcata
     3121 taactgacat tccagctcgt tttggaagtg gctggacagg atatagttgg aatagaaact
     3181 taataccaaa tccagaacag ttattgcaac aacttcatga tagaaagtta aaactctcct
     3241 taaatgtcca tcctgctgat gggatacggg cttatgaaga agcttatcct caaatcgcaa
     3301 aacgattggg gttaaatgta gaactagaag aacctgctat ttttgatttt tttaatccct
     3361 cttttaggga agcctacttt aaagatgttc attatgagct agaaaagcag ggagtagatt
     3421 tttggtggat tgactggcaa caagggacac aaggtatgct agatccactt tggcttttaa
     3481 accattatca ctatcaggat agttgtaaaa atgcagaagg tggtttgatt ttatcaagat
     3541 atgcaggtcc tggtagtcac cgctaccctg ttggtttttc aggggatact attattagtt
     3601 ggaattcctt aagatttcaa ccctatttta cagcgacagc atctaatatc ggttatagtt
     3661 ggtggagtca tgatatcggt ggacatatgt tgggggatta tgacgaagag ctacaaacta
     3721 gatggctaca gtttggtgtt tttagtccga ttactcgatt acatagttct agaagtcctt
     3781 ttaatagtaa agaaccttgg tttttttcag aaacaacatc taagattatg aagaaatacc
     3841 ttcgtttgag acatcagatg attccctatc tatataccat gaatgtaaag acacatgagg
     3901 aaggtgcccc attaatcagt ccaatctatt atttctatcc agagaatgat gagagctata
     3961 atgttccaaa ccaatacttt tttggaacag aactgatggt ggctcccatt gtagaaaaga
     4021 tggatttgac attccaatct gcaaaagtag atgtatggtt ccctgaaggt gaatggtatg
     4081 acttcttttc agagaaaaaa tacacaggtg gtgtgaagtt aagtgtttat agggacatct
     4141 cgactacgcc tgtgtttgca aaaagtggtg caatcattcc cttggttggt tctgagatag
     4201 gtatgggtgt tgatttacct gaagttgtag attggtatgt attcccaggg aaacaacatt
     4261 cttttgaaat gcttgaagat caaaatggtc aaagatataa aacaagatta tcaatcgatt
     4321 gggaaatggg aatggtagag ttagcattac aaggagattc tagtatcgtt ccaagcaata
     4381 gaaaacatag aattcatttt aaaggaacga atgtgtctat aattgaattg ccaaataaga
     4441 atgatacagc taaatttgaa tggaaagata ataaaaggac atctttaaat gacgaagttt
     4501 ttagactact aaagacagct tctcttccat atgaattaaa agatagattg ttaaatcaat
     4561 tcatcaatgc caaaaactct catgacttaa tgaatatctt gcatcatcag gataaggaat
     4621 tgagagggcg tttgttggaa atgatcttta ctagccaaaa ctaattgaaa taattagaaa
     4681 gtcatttgtt tagtgtatat gaagattctg tatggtattc gttatcactg gtcacattga
     4741 aattgtgaaa taagcaccta caacatagac tttacaacac ttcatgtttc atatcatggg
     4801 gtgtttttct atttaagtca aagccaatca aaaaatctca ctattcatca ggttaaagga
     4861 aacctaatca tagtgagatt gtgaattata gtagagtttg tatcgcctct tcaatttgtt
     4921 ttgggtctgt ttttgaagaa aagcgttcaa agacttgccc atctcgaccg atgagaaact
     4981 tagcgaaatt ccattcgact cgttttccta gtgggccaga tttctggtct tttaaccaga
     5041 catagagagg gtctgcttcc ttaccgttga ccttaatctt ggcaaaacgt gggaaggtgg
     5101 tttgaaaatg taggctacag aaggcgttga tttcctctgc gctgccgggt gcttgtccca
     5161 taaactgatt gcaagggaaa tccaatattt cgaagccctg atcttgatag cgttcataga
     5221 gttcttgaag tccctggtac tggggcgtta aaccacatcc agtagcagtg ttgacaatca
     5281 agagaacttt gccacgatag ctatccaagg gagttgcttg gttgttttgg ttcaaaacgg
     5341 aaaaatcata aagtgaagtc atgagggcct cttttctttt tctacattgt aacagttttt
     5401 acttttttcg tcgagtcgga tggaagatat tgttgaagaa taagccctca atatgataga
     5461 aatcgtttac aaataacttt aaaaatggta tgatttaact aaaccggttt acttagttgc
     5521 tataaatctc taatttgaga tttagttcct attgatttta caatatgttt attggagtgt
     5581 atacatgcaa acaaaaacaa agaagctcat tgtgagtttg tcttcacttg ttttatcagg
     5641 atttttatta aaccattata tgacagttgg agcggaagaa acgactacga ataccattca
     5701 gcaaagccag aaggaagttc agtatcagca aagggataca aaaaatttag ttgaaaatgg
     5761 tgattttggt cagacggagg acggaagcag ttcgtggaca ggaagcaaag ctcaggggtg
     5821 gtcagcttgg gtagaccaga agaatagttc tgcagatgcc tcaactcgag tcattgaggc
     5881 taaggatggg gctatcacta tctcaagcca tgagaaatta agggcagcgc ttcaccgtat
     5941 ggttcctatt gaagttaaga aaaagtataa actgcgtttc aagattaaaa cagataataa
     6001 aatcgggatt gccaaagttc gtatcattga ggaaagtggt aaggacaagc gattgtggaa
     6061 ttctgcaacg acgtcaggaa caaaggactg gcagaccatt gaagcagact atagcccgac
     6121 tttagatgtt gataaaatca agctggagtt attctatgaa acaggaactg ggactgtttc
     6181 ctttaaggat attgagctgg tagaggtagc agcccagctt tctgaggatt ctcaaacaga
     6241 taaacagctt gaggaaaaga ttgatttacc aattggaaaa aaacatgttt tttctcttgc
     6301 ggactatact tataaggtag aaaatcctga cgttgcttca gtcaaaaatg gaattttaga
     6361 acctcttaag gaagggacaa ccaatgtcat tgtcagtaaa gatggcaagg aagtgaaaaa
     6421 gattcctttg aagattctag cctctgttaa ggatacatac acagaccgtt tggatgactg
     6481 gaatggcatc atcgctggga atcaatacta tgattctaaa aatgaacaga tggccaaatt
     6541 aaaccaggaa ttggaaggaa aggtagctga tagcctatcc agtatttcaa gtcaggcgga
     6601 ccgcacctat ttgtgggaaa aattttcaaa ttataaaacg tctgcaaatc tgactgccac
     6661 ttatcggaaa ttggaggaga tggccaagca agtgaccaat ccttcttctc gttattatca
     6721 agatgaaact gtcgttcgaa cagtcaggga ttccatggaa tggatgcata aacatgtcta
     6781 caatagtgaa aagagcattg ttgggaactg gtgggattat gaaatcggta cacctcgtgc
     6841 catcaacaat accttgtctc tgatgaaaga atacttctct gatgaggaaa ttaaaaaata
     6901 tacagatgtg attgaaaaat ttgtaccaga tcccgaacat ttccgaaaga cgactgataa
     6961 cccatttaag gctctaggtg gaaacttagt tgatatggga agagtaaaag taatagctgg
     7021 tttactgcgt aaggatgatc aagaaatttc ttctaccatt cgctcgattg agcaagtgtt
     7081 caagttggta gaccaaggtg aaggttttta tcaagatgga tcctatatcg accacaccaa
     7141 tgttgcctat acgggtgctt atgggaatgt tttgattgat ggcctgtctc aactgttgcc
     7201 agtcattcaa aagaccaaga gtccaatcga taaagataaa atgcaaacca tgtaccactg
     7261 gattgataaa tcgtttgctc ctttgctggt gaatggagag ctgatggata tgagtcgtgg
     7321 acgctcgatc agtcgtgcaa atagcgaggg gcacgtggcc gcagtagaag tactaagagg
     7381 gattcaccga atagcggata tgtctgaagg agaaaccaaa caacgtttgc agagtcttgt
     7441 gaagaccatt gttcaatcgg atagttatta tgatgtcttt aagaatttga agacttataa
     7501 ggatatcagt ttgatgcaat ccttgttaag tgatgcagga gttgcaagtg ttccaagaac
     7561 aagttaccta tctgccttta acaagatgga taaaacagcc atgtacaatg cagagaaagg
     7621 gtttggattt ggcttgtcac tcttttccag tcgtaccttg aattacgaac acatgaacaa
     7681 ggaaaataaa cgtggttggt atacgagtga tgggatgttc tatctttaca atggcgattt
     7741 gagtcactat agcgatggct actggccaac agttaatcca tataagatgc ctggtacaac
     7801 agagacggat gctaagagag cggatagcga tacaggtaaa gttttaccgt ctgctttcgt
     7861 tggaacgagc aaactagatg atgccaatgc gacagcaacc atggatttca ccaactggaa
     7921 tcaaacattg actgctcata agagctggtt tatgctaaag gataagattg cctttttagg
     7981 aagcaatatc caaaacactt caacagatac tgctgcaact acaattgacc agagaaaact
     8041 ggaatcaagt aatccatata aagtctatgt caatgataaa gaagcctccc ttacagaaca
     8101 agaaaaggat tatcctgaaa cccaaagtgt ctttttagaa tcgtccgatt cgaaaaagaa
     8161 tattggttac tttttcttta agaagagttc aatcagtatg agtaaggctt tgcaaaaggg
     8221 agcctggaag gatatcaatg aaggacagtc agacaaggaa gttgaaaatg aatttcttac
     8281 gattagtcag gctcataagc aaaatggaga ttcttatggc tatatgctca ttcctaacgt
     8341 ggatcgtgcc accttcaatc aaatgataaa agagttagaa agtagcctca tcgaaaataa
     8401 cgaaaccctt cagtctgttt atgatgccaa acaaggagtt tggggcattg tgaaatatga
     8461 tgattctgtc tctactattt ccaaccaatt ccaagttttg aaacgtggag tctatactat
     8521 tcgaaaagaa ggggatgaat ataagattgc ctactataat cctgaaactc aggaatcagc
     8581 tccagatcag gaagtcttta aaaagctaga gcaagcagct cagccacaag tacagaattc
     8641 aaaagaaaag gaaaaatctg aagaggaaaa gaaccattcg gatcaaaaga atctccctca
     8701 gacaggagaa ggtcagtcaa tcttggcaag tctagggttc ttgctacttg gggcgtttta
     8761 tctattccgt agaggaaaga acaactaatt tgttcatcat ctagatgaat tacatgaaac
     8821 tgtggaaaat agaaaaaatc cgaagaaggc caaatatctt cggatttttc ttttaaaaaa
     8881 ggataagttg atacttatcc ttttttactt cattattatg tgactatagc gttttgaaac
     8941 tagaatagga cgttataact actaaaagat ttctataaat tcatttgatt ttcctaatca
     9001 atttgttcgt atcctatttc actccactat attagtccgt ctcgctccgt gaggtgcgag
     9061 acaaaacaac cacccgctat gcgggtgcgc gtcgaaggtt ataccaaaaa aactccaaac
     9121 gcgatacaat aaaggtgttc aatccaattg taaagcgaaa ggagaaaaaa tatggcacaa
     9181 aaggctcata gtttatctca cacaaagtgg aggtgttcta tcacattgtg ttcaccccta
     9241 agtatagacg aaaagtcatc tataatcaat atagaagtag tttaggcgaa atatttcatc
     9301 gcttgtgtag ttataaaggt gttgaaatga tcgagggtca cttaatgcca gaccatgtac
     9361 acatgttagt cagtattccg tcgaggctaa gtgtttcaag ttttatgggg tatttaaaag
     9421 gcaaaagtgc actcatgatg tttgacaaac acgccaatct caagtacaag tttgggaatc
     9481 ggcatttctg ggcggaaggt tattatgtaa gtccagtagg gcttaatgaa gccacaatta
     9541 agaaatatag tcaagattaa agaaattatc cagtggatga tttcttcacg agtatgaaaa
     9601 tttgaaaacg agtaaagtat gatatatagt aaaatgaaat aagaacagga caaaatcgat
     9661 caggacagtc aaatcgattt ttaacaatgt tttagaagta gaggtgtact attccagttt
     9721 caatccacta tagcactaga taaattgagt gtaaaagaat atgaggatcc ctttagggat
     9781 agtggtaagt aataccaaag tctcttaaag aggcaagtga cgagtcaaga gcaataaggc
     9841 ttgaacaacg tgaaagccag cgtctttagg cgctggctgg taatttgggc ttatagctct
     9901 gagataaacc acccgttaga cgggtagtta tgattttatc tgagtgtaat atactgttgg
     9961 gcaatctcgc tgatgcggtc aaagttgcct tgggaagcga gtttattgag ttcgccacca
    10021 attccaacgg catctgcacc agcagcgaac cattgaggga tgttgtttag gccgactcct
    10081 ccggttacca ttacggaaac ttgtgggatc ggtgccttga ctgcagagat atatgctgga
    10141 ctgagagtac tacctgggaa gagtttgatg atttcactac cggcttcaag tgcagtcgtg
    10201 atctctgtga gggtaataca gcctggaatg tacggtgtgc tgtagagatt gcacattttc
    10261 gcagtttcag catggaaaga tggagaaaca acgtaatttg ctccagctag aatggcatct
    10321 ctagcagtta cggcatcaag cacagtacct gcaccgatac aaacactctg atcgtcctga
    10381 tacaagtcta caagttcctt gatgatttgt cctgcatact gattggtata ggcgatttca
    10441 atagctttga taccgccctt gatacaagca atcgaggctt gtagtccttc ttcctttgta
    10501 tttcctcgaa tgacagcgac aattttctgt ttttttagtt caataatcgt atctgatttg
    10561 gtcatgtaat tctcctaacg aatgatatct tgtgcatttg ccagtaaatt ttcaatacta
    10621 gttgcggaag tggagagatg gtctcctgga agagtgcatt tgagagttgc gctcgcaact
    10681 gcaaagtcaa tggtagtttt tagggaggaa tgatggagta gttggtaaag ggcaccagat
    10741 ataaaggcat ccccgctacc aattcgttga tagactgcag tttttagttg gacagactct
    10801 tcaaatcgtt cttctagagc ataggcttga tagacatttt tgtcttgctc atcactagag
    10861 cggagggtat ggaaaatggc cttgaaacca taggcgtctt ttaaaagtcg catgcgattt
    10921 tccacctctt ctaggctagc actgtctctt ggaaacatct ctagattttg gtcatcaatc
    10981 atgagaggat caataccgaa gcaatagtca gtaaaacgtg caaacttaga aaattcatac
    11041 ttggcttcta ggactgaaat catctttgtt ctcagattga gatccattga aacgacaatt
    11101 cctcggcgct tggcttcttc caagagtagg agaaggatcg cacggacctc ttgaccgata
    11161 gctacggtga ttccactaaa atgaaaatgg ctaatcccct gaaagagaga atccatatct
    11221 agcatgtttg gccgaatctg gctgatactc gtatgcttac gatcgtagaa aacttcactt
    11281 tgacgacaac caaagccgtt ctccaaatag tagaggccga ttcgatcgcc aagccgacag
    11341 attgaactgg tatcgatttg gtgctgtttc aagaatgtga gaaaacgatc tccaatctcg
    11401 ttggcaggga gtgcggtaaa aactttcgtt gagataccca gggcttgcaa attacaagcg
    11461 atgttaattt ctgatccgcc aaaataagtc gaactggcaa catgatcgcc gatactggtg
    11521 gcatctaatg gtgaaattcg aattaatggt tcgccaaaca gtaatatttt cgtcatccat
    11581 tagttccttt ctggtttaga atagctttac ttggctaata cttctttcaa ataagcagcg
    11641 acttcatcat cttggcagtt ttcaaagaag tattcttgga agtatttgcc agagatggtg
    11701 tctttgagga agtcttggtc gattatttta aggatggtca tcaaatcatt gtgggtgatt
    11761 tgtttcacct cgtttaagat acgagcgttg cgttgttcag ggattacacg ttctcttggg
    11821 tagccaccgc ccatttcttc agcaaataag cgttcaaaca tcaatttgag ggtcagttct
    11881 gccccccagc caaatccttt ggcataaggg atagacaagg cgttaccacc attgatttga
    11941 gaataaaggt aagcgtcagt tgggtccact gctagaccac agacaacacc agggaagctg
    12001 tttaaagcaa gcatagcccc tacacccgta ccacagccgg taacaacaaa gtcaactgcc
    12061 tttgtattta aaaggatggc agccattagt ccgttctgca cataagttaa ttgactttct
    12121 ccttcttctc cacgcatacc atagttaaat aattggtagc cttttttatc tgtcgcttct
    12181 tttagactat cgtaaataat gtgattcttg ctagcttgac tattttcatt gattaatgcg
    12241 attttcataa gatcttcttt ctatttttac tcaggttgtt ttccgatgta ggctaagata
    12301 ccgccatcta catataggat gtggccattg acaaaattgc tggcatcact agcgagaaag
    12361 acagcagggc ccatcaaatc ttcagtattt ccccaacgtg cagcaggtgt ttttgcaatg
    12421 atgaactggt caaatgggtg gcgagaacca tcttcttgca attcacgaag aggtgctgtt
    12481 tgaggagtgg caatataacc cggtccaatt ccgttacatt ggatattggc tccaccgtat
    12541 tcagacgcaa tgttgcgggt caacattttc aagccccctt tagcagcagc ataagcgcta
    12601 actgtttcac gtcccagttc gctcatcatc gaacaaatat tgataatctt tccatgccct
    12661 ttctttatca tagaaggaat aactgccttt gaaacgataa atggtgcgtt taagtcaata
    12721 tcgatgacct tacggaaatc agcggcgctc atttcgcaca ttggaactcg gcggataata
    12781 ccagcgttat taacgaggat gtcaatgaca ccaacctctt gttcgatttg cttgaccatg
    12841 gcttggatac cgtcctcgtc tgtcacgtca cagacatatc catgggcttg gatgccaact
    12901 tcacgataag cttcaatccc tttattgacc agatcttgat tgatatcgtt aaagacaata
    12961 gtagcgccgg cttcagcgta ggatttggca atagcaaatc caattccata agaagcgccg
    13021 gtgatgagag caatttttcc ttttaaagaa aactgctcaa ttgagaatga tgtatttgtc
    13081 atttgatact tcctttcaaa tttgttaaga cttctctata accaaattat attctagtaa
    13141 accggttttg tcaataaata aatattttta tttataaaaa ggtttgtttt cctaaaaaaa
    13201 cggaagattt tgaaatttct gtctattaaa ctggttaaaa agataatgaa agcgctaata
    13261 tttttttaaa aaaactattg acaagataaa aataaaggat tacaatgggg aatataaagt
    13321 aaaccggtaa acctaaaaag aaaggagaaa agatgaaaat tgtacttgta gggcatggac
    13381 attttgctac agggatttat agttctttac aattgattgc aggtaatcaa gaaaatgtgg
    13441 aggcgattga ctttgtggaa ggaatgtcag cagatgaact caagcaaaaa atcttacttg
    13501 caatttcaaa tgaagaagaa gttttaatcc taagtgatct cttgggagga tcgccattca
    13561 aggtttcttc taccataatg ggagaaaatc cagccaagac aatgaatgtt ctctcgggtt
    13621 tgaacttagc catgttaatg gaagcagtct ttgctagaat ggctcatagc tttgatgagg
    13681 ttgttaataa atcagtagtg gcggcccagg gcggagtcgt aaatggtaaa gaattgtttt
    13741 caacggatgc agaggaagag gaagaagatt tcgaatcggg tatttaaagg gtaaaagaat
    13801 gataaaaaag gttacgattg aaaaaataaa atcgcctgag cgcttcttag aagtaccact
    13861 tctgacgaaa gaagaagtcg gccaggcaat cgataaggtt attcggcagt tagaactcaa
    13921 ccttgactat ttcaaggaag atttcccgac gccagctacc tttgataatg tctatccaat
    13981 catggataac acggaatgga ccaatggttt ctggacagga gaactgtggt tggcttatga
    14041 atacagtcaa caggatgcat ttaaaaacat cgctcataaa aatgttcttt ctttcctgga
    14101 tcgtgtcaat aagagagtag aattggatca ccatgatctc ggcttcttgt acacaccgtc
    14161 ttgtatggct gaatataaga taaatggaga tggagaggct agagaagcaa ccttgaaagc
    14221 tgcagataag ttgattgaac gctatcaaga aaaaggtggt tttattcaag cttggggaga
    14281 cttgggcaag aaagagcatt accgtttgat tatcgactgc ttgctcaata tccaactctt
    14341 attctttgct tatcaagaaa caggcgatca aaaatactac gatattgcag aaagccattt
    14401 ctatgcttca gctaataatg taatccgtga tgacgcttcg tccttccaca ccttctattt
    14461 tgatcctgag acaggtcaac cctttaaagg tgtaacgaga caagggtata gtgatgattc
    14521 atgctgggca cgtggtcaat catggggagt ctatggtatt cctttgactt atcgtcactt
    14581 aaaagacgag tcctgctttg acttgtttaa gggtgtgacc aattatttct tgaatcgtct
    14641 gccaaaagat catgtgtcct attgggattt gatttttaat gatggtagtg atcaatcacg
    14701 agattcttca gcaacagcta tcgccgtctg tgggattcat gaaatgctaa aacatctccc
    14761 agaggtggat gctgacaaag atatttataa acatgctatg catgccatgc ttcgttcctt
    14821 gatcgaacat tatgcaaatg atcaatttac ccctggtggg acaagtctcc tccacggtgt
    14881 gtactcatgg cattcaggta aaggagtgga tgaaggcaat atctggggtg actactatta
    14941 cctagaagcc cttatccgtt tctacaaaga ctggaaccta tattggtagg aggagaaata
    15001 tgacaatgcc aaatattatt atgacccgta tcgatgaacg gttgattcat ggacaaggac
    15061 aactttgggt aaaataccta ggttgtaata cggtcattgt tgccaatgac gaagtaagca
    15121 cggacaagat gcaacaaact ctgatgaaaa cagttgtgcc agactcagtt gccatgcgtt
    15181 tcttcccttt gcaaaaggtg attgatatca ttcacaaggc taatcctgct caaacgatct
    15241 ttatcgttgt aaaggatgtg aaggacgctt taaccttggt agaaggtggt gtcactatca
    15301 aagaaatcaa tattgggaac attcacaatg cccctggtaa agagcaagtg acacgctcca
    15361 tcttcctggg tgaagaggac aaggcggccc tcaaggaatt gagccaaact catcaagtaa
    15421 catttaatac gaaaacaact ccaacaggaa atgatggagc tgttcaagtc aacattatgg
    15481 actatattta aaagaggaga tcgttatgtc gattaatgta tttcaagcga ttttaattgg
    15541 attatggaca gctttctgtt ttagtggaat gctgttagga atttacacca atagatgtat
    15601 tgttctgtca tttggtgtcg gaattattct aggtgatctg cctactgctc ttgcaatggg
    15661 agctattggt gaattggctt atatgggatt cggtgttggt gctggaggta ctgttccacc
    15721 aaacccaatc ggacctggta tctttggtac cttgatggct atcactagtg ctggtaaagt
    15781 cagtccagaa gcggctcttg ccctctctac tccgattgct gtggcgattc aattcttaca
    15841 aactttcgcc tacactgtac gtgctggtgc gcctgaaaca gctatgaagc acttgaaaaa
    15901 ccataatttg aagaaattta agttcactct aaatgcaaca atttggttgt ttgcctttat
    15961 tggatttacc ttgggttgct tgggtgccct ttcaatggat accttgttga aactcgtaga
    16021 ctacattcca ccggtattac ttacaggttt gacagttgct ggtaaaatgc tcccagctat
    16081 cggttttgcg atgatcttgt cagtgatggc taagaaagag ttgattccct ttgtcttgtt
    16141 gggatatgtt tgtgcagctt atctaaacat cccaacaatt ggtattgcaa ttgtaggtac
    16201 tatctttgct ttgattgaat tttataacaa gccaaaaaca gcggatcatg tggtagagga
    16261 ggaagcacac gatgactgga tctaacaaat taacaaaacg tgattatctt aaaacgtctt
    16321 tgcgggcatt cttttgtcaa aatggattta actatagtaa ctatcaaggg ttgggatatg
    16381 ccaatgtgat gtatcctgct ttgaaaaaac actatggaga ggatcaggaa ggtttctacc
    16441 aagccttgga agaaaactgt gaattctata ataccaaccc acacttcctg ccttttatta
    16501 ccagcttgca tcttgtaatg ttggaaaatg gccgtccggc aaaagaaaca cgtagcatca
    16561 agatggcctt gatgggacca ttggcaggta ttggggattc tctttctcaa ttctgtttag
    16621 ctccattgtt ctcaaccatc gcagcttcgt ttgctcaaga aggcttggtt gtcggtccaa
    16681 tcttgttctt ccttgcgatg aatacgattt taacagcgat taaattgtca actggtctgt
    16741 atggatacaa actaggaaca actgtgattg ataaactaag cgaacagatg gcaacgattt
    16801 ctcgtattgc caatattatc ggtgtaaccg taattgctgg tttggcagcg acatctgtta
    16861 agattatggt gccgattacc tttgctgcag gagaagttaa agcagacgct aaacaaagta
    16921 tcgtaagtat tcagggaatg cttgataagg ttgctccagc tcttctacca gccctattta
    16981 cacttttagt ttactacttg atcaaagaaa agaaatggac aacatataaa ctcgttattt
    17041 taacagttat catcggaatt atcggaagct ggcttaagat tatagcttaa tggatacaac
    17101 attgttttat ggaatagtga ttgtcttggc agtgagtccc cttttactgt caagctttca
    17161 ttctattcgt cagcaaaagt tgcttcgcaa acagatggag caacgacaag agtatttagc
    17221 ttctttaaca tctggtgatg aagtgttgtt actgtctgga attcatggaa aaatcatttc
    17281 tatcaaagat gacttggtct ccttgcagat tgcaaaaggt gtggtcatct atgtagaaaa
    17341 ggaaagtgta atgggaaaga caaaagaact gctttttaag tagttctttt tctggtaata
    17401 gataatgaaa gaagaaagaa gacaattttt tgaaagagtc gatggaaacc aatgtcgtga
    17461 ttatatcttg tcgcactgtt caaaagacta tgagaaagtc aagagttccc ttgaacgctt
    17521 gatgaacaat cgttttatgt ttgatagtcc ttgggatatg gagccttgtt caaaaatcca
    17581 tcaaatccag ccgatggtat gggatcaagt atttgaagat gatccagaat gggcttatat
    17641 gctcaatcga caagaatatc tcttgcagtt tatgataggg tatctggtag aaggagataa
    17701 gagctatatt caaaagtgca agttctttct atttgattgg attgagcagg tgagagaatt
    17761 ttctcctcaa tccttgatga ctagaacctt ggatacgggt attcgttcct ttacttggtt
    17821 gaaactactc ttgctcctct tgaaatttga cttgctagag gagaaagaac tagagaaaat
    17881 tttggtcagt ctagaaaagc agattgactt tatgaaaagc tactatcgcg ccaagtacac
    17941 ccttagtaac tgggggattt tacaaacaat tccgatgctt gctatctatc atttcttttc
    18001 agataagatg gacctagaag aagcttatca ttttgcttca gaggagttga aacagcaaat
    18061 tgagacacag attttaggag atggaagcca gtttgaacag tcgattctct atcatgtaga
    18121 ggtttataaa gccttgctgg atttgtgtct cttgcttcca gacttgcaag atagttacca
    18181 agagttgctg gaaaagatgg cgacctatat tcaaatgatg acaggcttag atggacggac
    18241 tttggctttt ggtgatagcg attctacaga aacgacagaa attttgagcc tgtctgctgt
    18301 ggttttgaac aaggaagacc ttcttaacgg tctgtatgtt aaagtcgatt tgcttagcct
    18361 cttgttcctg gggcgagaaa aggtcaagcg actgcaggaa tttgaaaaga gagcttggca
    18421 gcctaggtcc atgatctttg aagactctgg acatgtctgc attaaggatg aacatcgtta
    18481 tctatttttc aaaaatggtc cgctaggaag tgcccatagc catagtgacg agaatagttt
    18541 ttgcttacag tatcaaggct aacctatttt catagatgct gggcgttatt cttatcggga
    18601 gatatatgaa cgttatctct taaagagtgc ttggagtcat tcgacctgca ttgtagatgg
    18661 gaaagctccg gaaagaatca cgggatcctg ggaatatgaa tactatcctc actccctgtt
    18721 ttgtcaccat aaagaaaggg agggagtgca ttatattgaa ggggcttatt ggtcagcaga
    18781 acctgatttg ccttatcttc acaagagaaa aatcctcatg ttggtagagg atgtctggct
    18841 cttggtagat gacatcaggt gtcaaggtca gcatgaggcg ttgactcagt ttatccttga
    18901 caaggatgtg acctatcaag atgggaaaat caatcagttg agactatgga gtgaagttga
    18961 ttttgatttg gaagatacca tcatttctcc taaatacaat gagcttgaaa gaagtagcaa
    19021 actcaccaag cgccaattct ttgagaatca gatgctggat tataccatca ttgcgcatga
    19081 gagttttgaa atcatccgtc attctgtcta ccagacagat gatcgtgaag tggaaaatgc
    19141 tctggctttt gaagtaaaaa atgacgaaac agacaagctg attctgttat taagcgagga
    19201 tatttgtgta ggtgaaaaat tgtgcctcgt tgacggaaca aaaatgcgtg gaaaatgtct
    19261 agtatatgat aaaataaatg agagaatgat tcgcttgcag tgctagaaat aggcattttg
    19321 aatagtgaat atgttataat aagtattagt aggaggtgtt ttagattgga gaagaaactg
    19381 accataaaag acattgcgga aatggctcag acctcgaaaa caaccgtgtc attttaccta
    19441 aacgggaaat atgaaaaaat gtcccaagag acacgtgaaa agattgaaaa agttattcat
    19501 gaaacaaatt acaaaccgag cattgttgcg cgtagcttaa actccaaacg aacaaaatta
    19561 atcggtgttt tgatcggtga tattaccaac agtttctcaa accaaattgt taagggaatt
    19621 gaggatatcg ccagccagaa tggctaccag gtaatgatag gaaatagtaa ttacagccaa
    19681 gagagtgagg accggtatat tgaaagcatg cttctcttgg gagtagacgg ctttattatt
    19741 cagccgacct ctaatttccg aaaatattct cgtatcatcg atgagaaaaa gaagaaaatg
    19801 gtcttttttg atagtcagct ctatgaacac cggactagct gggttaaaac caataactat
    19861 gatgccgttt atgacatgac ccagtcctgt atcgaaaaag gttatgagca ttttctcttg
    19921 attacagcgg atacgagtcg tttgagtact cggattgagc gggcaagtgg ttttgtggat
    19981 gctttaacag atgctaatat gcgtcacgcc agtctaacca ttgaagataa gcatacgaat
    20041 ttggaacaaa ttaaggaatt tttacaaaaa gaaatcgatc ccgatgaaaa aactctggta
    20101 tttatcccta actgttgggc cctacctcta gtctttaccg ttatcaaaga gttgaattat
    20161 aacttgccac aagttgggtt gattggtttt gacaatacgg agtggacttg cttttcttct
    20221 ccaagtgttt cgacgctggt tcagccctcc tttgaggaag gacaacaggc tacaaagatt
    20281 ttgattgatc agattgaagg ccgtaatcaa gaagaaaggc aacaagtctt ggattgtagt
    20341 gtgaattgga aagagtcgac tttctaaaat gaaggaaaat gacttgcaat ctctgttaag
    20401 aaataaaata atcccaccta gaacaagcta ggtgggatta tttgcttatg aaatgagaaa
    20461 ttatgggagc aagctcctaa atcaactgtt tttgatctac ttctttaact acttgataaa
    20521 agttatagaa gtaggccaaa cttgaaatga tggttacgac taggaatatt gaaaatttcc
    20581 attggacagg gttggttaaa agttgtggaa aggatatgag gagaaagaag agggctgcgt
    20641 tgaggacagg tatccgtttt gattgtattt tctcaagtcc tttattgagc gcaggaagaa
    20701 agaggagtag gagtagtaaa actgtatgag aaacagctcc tgaaataagg gcgaagaaaa
    20761 ggaaaatact gataaaaaca tgaatgttca gtagtctagc tagtgatttc ataaggcacc
    20821 tcctaatcct ggtctttttt agctcttgca atacgaagtg agtcgacaat atgtatcatc
    20881 actccgaaaa agaaagctcc cagtatagtt ttaaaaatat gttttgtatt tagaagagaa
    20941 ctgataaaat ttggattttc acttgttagg gtatcaatga gtggaattat aaaaaatatc
    21001 actgttccat aaatcgaacc tgctttaaga ccaggataac gtaactgttt cttttctttt
    21061 ttcatgagtt tcctcctaat cctcatcttg atttttctta gtttttgcaa tgcgacggga
    21121 gatgaggaac tgtatgctcg ctccgaagaa aatagaaccg agaatacttg atacaccatt
    21181 tcttatagtg agaagagaat gaaaatagtc ctgaccttca tctatgagta tactgagaag
    21241 aggagttata aaaaacatcc atagaccaaa gaacaaacct gctttcagac ctgggtagtg
    21301 tagttgcttg ctttctttct cattcagcat atctggttca atgactgtga tgcctgtttt
    21361 tttcatttgg taggtgacat agccagcagc gatgagggca atcactaaaa tcagaggagg
    21421 atagattaga gccacttctt gagggtattt ataggctaga aggagtggaa taagatttcc
    21481 gaaaatcatc agataaaaga ggacgataaa gacttggttc ccaatgctat cggcctcacg
    21541 ccgtttgtat tcgtcaaggg gaccagaaat accgtatgtg cgtttgatca gtttttcagt
    21601 gaaggtttct tttttcatga gtttgctcct tttttaaaaa tcttcctccc aaaagagact
    21661 gttgaggtca gtttggaggc tgcgggcgag attgagacag agttccaagg ttggattgta
    21721 cttgtcgttt tcaatcatat tgatagtctg tctcgagaca ccgatatcct tggcgagttc
    21781 gagctgggaa atacccaatt ccttgcgaaa ttctttcaca cgattcatct ggtctccttt
    21841 ctgatttatg tcgtatatat ttgactatat tatagtcttt taaacataaa gtgtcaagta
    21901 tatttgacat attttttgaa gaaatagtag tctccttgtc ctatttgtct gacaagtgca
    21961 agctggtcgg atttgtggta aaatagataa gatatgacaa aagaatttca tcatgtaacg
    22021 gtcttactcc acgaaacgat tgatatgctt gacgtaaaac ctgacggtat ctacgttgat
    22081 gcgactttgg gtggagcagg ccatagcgag tatttattaa gtaaattaag tgaaaaaggc
    22141 catctctatg cctttgacca ggatcagaat gccattgaca atgcgcaaaa acgcttggca
    22201 ccttacattg agaagggaat ggtgaccttt atcaaggaca acttccgtca tttacaggca
    22261 cgtttgcgcg aagctggtgt tcaagaaatt gatggaattt gttatgactt gggagtgtct
    22321 agtcctcaat tagaccagcg tgagcgtggt ttttcttata aaaaggatgc gccactggac
    22381 atgcggatga atcaggatgc tagtctgaca gcctatgaag tggtgaacaa ttatgactat
    22441 catgacttgg ttcgtatttt cttcaagtat ggagaggaca aattctctaa aaagattgcg
    22501 cgtaagattg agcaagcgcg tgaagtgaag ccgattgaga caacgactga gttagcagag
    22561 attatcaagt tggtcaaacc tgccaaggaa ctcaagaaga aggggcatcc tgctaagcag
    22621 attttccagg ctattcgaat tgaagtcaat gatgaactgg gggcggcaga tgagtccatc
    22681 cagcaggcta tggatatgtt ggctctggat ggtagaattt cagtgattac ctttcattcc
    22741 ttagaagacc gcttgaccaa gcaattgttc aaggaagctt caacagttga agttccaaaa
    22801 ggcttgcctt tcatcccaga tgatctcaag cccaagatgg aattggtgtc ccgtaagcca
    22861 atcttgccaa gtgcggaaga gttagaagcc aataaccgct cgcactcagc caagttgcgc
    22921 gtggccagaa aaattcacaa gtaagaggga aaaagatggc agaaaaaatg gaaaaaacag
    22981 gtcaaatact acagatgcaa cttaaacggt tttcgcgtgt ggaaaaagct ttttactttt
    23041 ccattgctgt aaccactctt attgtagcca ttagtattat ttttatgcag actaagctct
    23101 tgcaagtgca gaatgatttg acaaaaatca atgcgcagat agaggaaaag aaaaccgaat
    23161 tggacgatgc caagcaagag gtcaatgaac tattacgtgc agaacgtttg aaagaaattg
    23221 ccaattcaca cgatttgcaa ttaaacaatg aaaatattag aatagcggag taagatatga
    23281 agtggacaaa aagagtaatc cgttatgcga ccaaaaatcg gaaatcgccg gctgaaaaca
    23341 gacgcagagt tggaaaaagt ctgagtttat tatctgtctt tgtttttgcc atttttttag
    23401 tcaattttgc ggtcattatt gggacaggca ctcgctttgg aacagattta gcgaaggaag
    23461 ctaagaaggt tcatcaaacc acccgtacag ttcctgccaa acgtgggact atttatgacc
    23521 gaaatggagt cccgattgct gaggatgcaa cctcctataa tgtctatgcg gtcattgatg
    23581 agaactataa gtcagcaacg ggtaagattc tttacgtaga aaaaacacaa tttaacaagg
    23641 ttgcagaggt ctttcataag tatctggaca tggaagaatc ctatgtaaga gagcaactct
    23701 cgcaacctaa tctcaagcaa gtttcctttg gagcaaaggg aaatgggatt acctatgcca
    23761 atatgatgtc tatcaaaaaa gaattggaag ctgcagaggt caaggggatt gattttacaa
    23821 ccagtcccaa tcgtagttac ccaaacggac aatttgcttc tagttttatc ggtctagctc
    23881 agctccatga aaatgaagat ggaagcaaga gcttgctggg aacctctgga atggagagtt
    23941 ccttgaacag tattcttgca gggacagacg gcattattac ctatgaaaag gatcgtctgg
    24001 gtaatattgt acccggaaca gaacaagttt cccaacgaac gatggacggt aaggatgttt
    24061 atacaaccat ttccagcccc ctccagtcct ttatggaaac ccagatggat gcttttcaag
    24121 agaaggtaaa aggaaagtac atgacagcga ctttggtcag tgctaaaaca ggggaaattc
    24181 tggcaacaac gcaacgaccg acctttgatg cagatacaaa agaaggcatt acagaggact
    24241 ttgtttggcg tgatatcctt taccaaagta actatgagcc aggttccact atgaaagtga
    24301 tgatgttggc tgctgctatt gataataata cctttccagg aggagaagtc tttaatagta
    24361 gtgagttaaa aattgcagat gccacgattc gagattggga cgttaatgaa ggattgactg
    24421 gtggcagaat gatgactttt tctcaaggtt ttgcacactc aagtaacgtt gggatgactc
    24481 tccttgagca aaagatggga gatgctacct ggcttgatta tcttaatcgt tttaaatttg
    24541 gagttccgac ccgtttcggt ttgacggatg agtatgctgg tcagcttcct gcggataata
    24601 ttgtcaacat tgcgcaaagc tcatttggac aagggatttc agtgacccag acacaaatga
    24661 ttcgtgcctt tacagctatt gctaatgacg gtgtcatgct ggagcctaaa tttattagtg
    24721 ccatttatga tccaaatgat caaactgctc ggaaatctca aaaagaaatt gtgggaaatc
    24781 ctgtttctaa agatgcagct agtctaactc ggactaacat ggttttggta gggacggatc
    24841 cggtttatgg aaccatgtat aaccacagca caggcaagcc aactgtaact gttcctgggc
    24901 aaaatgtagc cctcaagtct ggtacggctc agattgctga cgagaaaaat ggtggttatc
    24961 tagtcggggt aaccgactat attttctcgg ctgtatcgat gagtccagct gaaaatcctg
    25021 attttatctt gtatgtgacg gtccaacaac ctgaacatta ttcaggtatt cagttgggag
    25081 aatttgccaa tcctatcttg gagcgggctt cagctatgaa agactctctc aatcttcaaa
    25141 caacagctaa agctttggag caagtaagtc aacaaagtcc ttatcctatg cccagtgtca
    25201 aggatatttc acctggtgat ttagcagaag aattgcgtcg caatcttgta caacccatcg
    25261 ttgtgggaac aggaacgaag attaaaaaca gttctgctga agaagggaag aatcttgccc
    25321 cgaatcagca agtccttatc ttatctgata aagcagagga ggttccagat atgtatggtt
    25381 ggacaaagga gactgctgag acccttgcta agtggctcaa tatagaactt gaatttcaag
    25441 gttcgggctc tactgtgcag aagcaagatg ttcgtgctaa cacagctatc aaggacatta
    25501 aaaaaattac attaacttta ggagactaat atgtttattt ccatcagtgc tggaattgtg
    25561 acatttttac taactttagt agaaattccg gcctttatcc aattttatag aaaggcgcaa
    25621 attacaggcc agcagatgca tgaggatgtc aaacagcatc aggcaaaagc tgggactcct
    25681 acaatgggag gtttggtttt cttgattact tctgttttgg ttgctttctt tttcgcccta
    25741 tttagtagcc aattcagcaa taatgtggga atgattttgt tcatcttggt cttgtatggc
    25801 ttggtcggat ttttagatga ctttctcaag gtctttcgta aaatcaatga ggggcttaat
    25861 cctaagcaaa aattagctct tcagcttcta ggtggagtta tcttctatct tttctatgag
    25921 cgcggtggcg atatcctgtc tgtctttggt tatccagttc atttgggatt tttctatatt
    25981 ttcttcgctc ttttctggct agtcggtttt tcaaacgcag taaacttgac agacggtgtt
    26041 gacggtttag ctagtatttc cgttgtgatt agtttgtctg cctatggagt tattgcctat
    26101 gtgcaaggtc agatggatat tcttctagtg attcttgcca tgattggtgg tttgctcggt
    26161 ttcttcatct ttaaccataa gcctgccaag gtctttatgg gtgatgtggg aagtttggcc
    26221 ctaggtggga tgctggcagc tatctctatg gctctccacc aagaatggac tctcttgatt
    26281 atcggaattg tgtatgtttt tgaaacaact tctgttatga tgcaagtcag ttatttcaaa
    26341 ctgacaggtg gtaaacgtat tttccgtatg acgcctgtac atcaccattt tgaacttggg
    26401 ggattgtctg gtaaaggaaa tccttggagc gagtggaagg ttgacttctt cttttgggga
    26461 gttgggcttc tagcaagtct cctgaccctc gcaattttgt atttgatgta agaatggcac
    26521 cctgatgttt cagggtgttt ttgtgtttaa atacacaatg aaaatcaaag aacaaactag
    26581 aaagctaact ttaggctgct caaaacatag tagtttgaaa ctagaatagt acacatctac
    26641 ttctaaaata ttgttagaaa tcgatttgac tgtcctgatc gatttgtcat gttcttattt
    26701 cattttaata tacagttttg agatgggtga tagaactgac gaagtcagct caaaacacgg
    26761 ttttgaggtt gtagataagg agaagctgat gtggtttgaa gaggttttcg aagagtgtaa
    26821 aaaaatattg gtcagttaaa gtcaaaactc ttgacctttt ctgaccaatg tagtatatta
    26881 tgaacgtaag gtaaatgaaa gccttactag aacacttatt taaagtaaaa tagaaagagg
    26941 tgggtctatg tttcatctag aaatcttcag aagtaaagat ggtctactcc tgcttgaaaa
    27001 agaaaaacca gaaatagtac gtagggtagc ggtttagcta gtctaaattt tttaaaacaa
    27061 tggtcaaaga tagtcaatat cagtaatcat aactaagtaa acaaaaagag gtaaagaata
    27121 tgaataacaa ctttaataat tttaataaca tggatgattt atttaaccaa ttgatgggtg
    27181 gtatgcgagg atatagttct gaaaatcgcc gttacttaat taatggacgc gaagtcacac
    27241 ctgaggaatt tgctcactat cgtgcgactg gtcaattacc aggaaatgca gaaactgatg
    27301 tgcaaatgcc acaacaggca tcaggtatga aacaagacgg tgtccttgca aaactaggtc
    27361 gaaacttgac agcagaagcg cgtgagggca agttggatcc tgttatcgga cgaaacaagg
    27421 aaattcaaga aacatctgaa atcctctcac gccgcaccaa gaacaatcct gttttggtcg
    27481 gagatgcagg tgttggtaag acagcagttg tcgaaggtct agcgcaagcc attgtgaacg
    27541 gagatgttcc tgctgctatc aagaacaagg aaattatttc tattgatatc tcaggtcttg
    27601 aggctggtac tcaataccgt ggtagctttg aagaaaatgt ccaaaactta gtcaatgaag
    27661 tgaaagaagc agggaatatt atcctcttct ttgatgaaat tcaccaaatt cttggtgctg
    27721 gtagcactgg tggagacagt ggttctaaag gacttgcgga tattctcaag ccagctctct
    27781 ctcgtggaga attgacagtg attggggcaa caactcaaga cgaataccgt aacaccatct
    27841 tgaagaatgc tgctcttgct cgtcgtttca acgaagtgaa ggtcaatgct ccttcagcag
    27901 agaatacttt taaaattctt caaggcattc gtgacctcta tcaacaacac cacaatgtca
    27961 tcttgccaga cgaagtcttg aaagcagcgg tggattattc tgttcaatac attcctcaac
    28021 gtagcttgcc agataaggct attgaccttg tcgatgtaac ggctgctcac ttggcggctc
    28081 aacatccagt aacagatgtg catgctgttg aacgagaaat cgaaacggaa aaagacaagc
    28141 aagaaaaagc agttgaagca gaagattttg aagcagctct aaactataaa acacgcattg
    28201 cagaattgga aaagaaaatc gaaaaccaca cagaagatat gaaagtgact gcaagtgtca
    28261 acgatgtggc tgaatctgtg gaacgaatga caggtatccc agtatcgcaa atgggagctt
    28321 cagatatcga acgtttgaaa gatatggctc atcgcttgca agacaaggtg attggtcaag
    28381 ataaggccgt agaagctgta gctcgtgcta tccgtcgtaa ccgtgctggt tttgatgaag
    28441 gaaatcgccc aatcggcagc ttcctctttg tagggtctac tggggttggt aagacggagc
    28501 ttgctaagca attggcactc gatatgtttg gaacccagga tgcgattatc cgtttggata
    28561 tgtctgaata cagtgaccgc acagctgttt ctaagctaat tggtacaaca gcaggctatg
    28621 tgggttatga tgacaatagc aataccttaa cagaacgtgt tcgtcgcaat ccatactcta
    28681 tcattctctt ggatgaaatt gaaaaggctg atcctcaagt tattaccctt ctcctccaag
    28741 ttctagatga tggtcgtttg acagatggtc aaggaaatac agtaaacttc aagaacactg
    28801 tcattattgc gacctcaaat gctggatttg gctatgaagc caacttgaca gaagatgcgg
    28861 ataaaccaga attgatggac cgtttgaaac ccttcttccg tccagaattc ctcaaccgct
    28921 ttaatgcagt catcgagttc tcacaattga ctaaggaaga cctttctaag attgtagatt
    28981 tgatgttggc tgaagttaac caaaccttgg ctaagaaaga cattgacttg gtagtcagtc
    29041 aagcggctaa agattatatc acagaagaag gttacgacga agtcatgggg gttcgtcctc
    29101 tccgtcgcgt ggttgaacaa gaaattcgtg ataaggtgac agacttccac ttggatcatt
    29161 tagatgctaa acatctggaa gcagatatga aagatggcgt tttggttatt cgtgagaaag
    29221 cctaagacag aattttgagg ataaaaaaga aggagccagc tgaaaaaact ggttcctttt
    29281 gaggtacgac gggcatgtcg tacagtaggt tgaaactaga atagtacaca tctacttcta
    29341 aaatattgtt agaaatcgat ttgactgtcc tgattgattt gtcctgttct tatttcattt
    29401 tactatatat ctgaggtgta agtcctcggt gggtacctgc taccggtgct cccaatagtg
    29461 attcccaagc ctgactatcg tgaggtagcg gattaaaacg gtctggggat agaccgtttt
    29521 aagtctgacg ctggaaataa gaatcgtcag aggaagggat agcgaaatcg tggctctacg
    29581 aataggaacg tgataataag gcgtatatag cggataaggg gcctcaaact ctaaagttca
    29641 aaaaggtagt cgtagcctat atgtgtaaat cacgagagca actgaatttg tctgttcgag
    29701 gatggataaa ctatttctca ttgggaaata tgaaaagtat agtcactagc atagatgagc
    29761 gcttgcatac tcgcctacga gtgattatct ggaagcaatg gaagaagaaa tcgagacgat
    29821 tatggggatt gcttaagtta ggagttccta aatggatagc agataaggta tctggctggg
    29881 gcgaccatta tcaattagta gctcagaagt cggtacttaa acgtgctata tcaaaaccag
    29941 tccttgaaaa acgtggactg gtttcgtgtt tggattatta ccttgaacga catgcgttaa
    30001 aagttagttg aaccgccgta tgccgaacgg cacgtacggt ggtgtgagag gggctagaga
    30061 ttatccccta ctcgatttta aatcacatga cgttcaaagg catcatctga aatcccttgt
    30121 tccaagatga gtcttgccca ttctttagca gagaagaggc tgtggtcctt gtagtttccg
    30181 caagattcga tggttgtccc tgggacatct tcccaagtag tagtttcagc gatttccttg
    30241 agcgaatcct tgataacagc tgcgatttta gcactggtgt gacgtcccca cataatcatg
    30301 tggaagcctg tgcgacaacc aaatggtgaa cagtcaatca tgccgtcaat gcgggtacgg
    30361 atgagtttgg ctaagaggtg ctcgatagtg tgaaggccgg cagtagggat agagtcttcg
    30421 tttggttgca ccaagcgaat atcataattg gagatgatgt ctccttttgg tcctgtttct
    30481 tccccaatca agcgaacata gggtgctttg acaatggtgt ggtcaagttc aaaactttcg
    30541 acaataactt cttttgacat ggtaaatcct ttcagttttc ttctctcatt atatcataaa
    30601 ggttgctcct gagacagaga gaaaacctct ccgaggctgg agaggttgaa atctttactt
    30661 acgatataag cggtcgtatt ggtagtatgg gtcaaaggtt acgttgatac ccagtttacg
    30721 aaggacattc ttgtcttcat cagtcaagat gatggttgag tgggcttcgc ttcctttgag
    30781 gttgccgagt tcttccatag cgcgggcagc atcaggattt tctgtagctg tgatagcaag
    30841 tgcaatcagg atttcatttg aatgaaggcg tggattgcgg ctaccgagat gatcgatttt
    30901 aagaccttgg attggcttaa caacttcagg ctcgattagt tttacttctt tagcgatgtc
    30961 agctgatttt ttgatggcgt tgatcaaggc agcggctgta ggcccaaaga gttctgagtt
    31021 cttaccagtg acgatttccc catttggcaa ttcaaaggct agggctggtc caccagtttc
    31081 ttctgctttt tggcgcgcaa cgacagcaac cttacggtct gcaggtgtga taccgaggtc
    31141 gttcatgagc aactcaattt tcttgacggc agcttcgcta actttttcag ctttgaagtc
    31201 aagaactgtt tgatagtaac ggcggatgat ttcttgttta gaagcttcga cagcagcttc
    31261 gtcatctgta atagcgaaac caaccatgtt gacacccata tctgtcggtg aagcgtatgg
    31321 tgattttccg agaatacgtt ccaacatgcg cttgagcact gggaagattt cgatatcacg
    31381 gttgtagttg acagtggttt ctccataggt ttgaagatgg aaggggtcaa tcatgttgac
    31441 atcatcaagg tcagctgtgg cagcttcata agccaagtta actggatgat gaaggggaag
    31501 attccaaaca gggaaggttt caaatttagc gtagccagac ttgatgccat tgatttggtc
    31561 gtggtacata ttggacatac acgttgccaa ttttccagaa ccgggtccag gagcggttac
    31621 gacaatcaag ttgcgactgg ttttgatgta gtcgtttttg cccatgcctt ttgaggaaat
    31681 gatgtgatcc atatccgtcg gatatccttt gattggataa tgaagataag aatcaattcc
    31741 gtttttctca agttggttgc ggaaggcatc tgcagcgggt tggccagcgt attgtgtaat
    31801 gacaacggaa ccaacaaaaa tccctaattc attgaatttg tcaatcaaac gaagaacttc
    31861 ttggtcataa gaaatgccta agtcgccacg tgctttggaa tgttcaatgt tactagcatt
    31921 aatggcaatc acaacctcaa tctgctcttt caattcttgc aagagcttga ttttgttgtc
    31981 aggttcataa ccaggaagga cacgagcagc gtggaaatct tctaacattt tgccaccaaa
    32041 ctctaggtaa agcttgccgt caaattggtt aatgcgctct aagatgtggt cgcgttgtag
    32101 atttaaatat tgttcagaac taaaagcttg ttttttcatt tttttacctc tggactctat
    32161 tataataaaa aattggaagt taggaaacta cggagctaaa aaagaaatta aaaagattaa
    32221 gcaaacgctt gcacaaaatt ttaaaaagtg ctatcatata ctatagatta ttaaaataat
    32281 gaggtaagaa gatgcaagaa aaatggtggc ataatgccgt agtctatcaa gtctatccaa
    32341 agagttttat ggatagtaat gaagatggaa ttggcgattt gccaggaatt accagtaagt
    32401 tggactatct agccaagcta ggaatcacag caatttggct ttctcccgtt tatgacagcc
    32461 ctatggatga taatggctat gatattgctg attatcaagc gattgcggct atttttggaa
    32521 ccatggagga catggatcag ctgattgcag aagctaagaa gcgtgacatt cgtatcatca
    32581 tggacttggt ggttaatcat acctcagatg aacatgcttg gtttgtcgaa gcctgtgaaa
    32641 atactgacag ccctgagcga gactactata tctggcgcga tgaacccaat gacctagatt
    32701 ctatctttag tgggtctgct tgggaatacg atgaaaagtc aggtcaatac tatctccact
    32761 ttttcagcaa aaaacagccg gatctcaact gggaaaatga aaaacttcgc cagaaaattt
    32821 atgagatgat gaacttctgg attgataaag gtattggtgg tttccgtatg gatgttattg
    32881 acatgattgg caaaattcct gacgagaagg tagtcaataa tggtcctatg ctccatccct
    32941 atctcaagga aatgaatcag gcgacctttg gagataagga tctcttgaca gtaggggaga
    33001 cttggggagc aacgccagag attgccaagc tctactctga tccaaagggg caagaattgt
    33061 ctatggtctt ccagtttgaa catatcggtc ttcagtatca ggaaggtcag cctaaatggc
    33121 actatcaaaa agagctgaat atcgctaagt taaaagaaat cttcaacaaa tggcagacag
    33181 agttaggagt tgaggacggc tggaattccc tcttctggaa caaccatgac ctccctcgta
    33241 ttgtctcaat ctggggaaat gaccaagaat accgcgaaaa atctgccaaa gcctttgcaa
    33301 tcttacttca tctcatgaga ggaactcctt atatctacca aggtgaggag attgggatga
    33361 ccaactatcc gtttgaaaca ctggatcaag tagaagatat tgaatctctc aactatgcgc
    33421 gtgaggctct tgaaaaaggt gttccgattg aagaaatcat ggacagtatc cgtgttattg
    33481 gacgtgacaa tgcccgtacc cctatgcaat gggacgagag caaaaacgct ggtttctcaa
    33541 caggtcaacc ttggttggcg gttaatccaa attacgagat gatcaatgtc caagaagcgc
    33601 tggcaaatcc agattctatt ttctatacct atcagaaact ggtccaaatt cgcaaggaga
    33661 atagctggct agttcgagct gactttgaat tgcttgatac ggctgataag gtctttgctt
    33721 atatacgtaa ggatggcgac cgtcgcttcc tagttgtggc taacttgtcc aatgaagagc
    33781 aagacttgac agtagaagga aaagtcaaat ctgtcttgat tgaaaacact gcggctaaag
    33841 aagtacttga aaaacaggtc ttggctccat gggatgcttt ctgtgtggaa ttactataaa
    33901 tattttttgc agaaaaattt aaaattgaaa tcatataaaa acaagggagg actgtataaa
    33961 agacagaaat cctttgtttt ttataaccaa ggtttataaa ctttcattct cgaaattcaa
    34021 ttaactttac aaattcccac tatttaagga gaaagaagat gaacataaag aagcgagtcc
    34081 ttagtgcagg cctgactttt gcatctgctt tgcttttagc tgcttgcggc caatcaggtt
    34141 cagatacaaa aacttactca tcaacctttg gtggaaatcc aactacattt aactatctat
    34201 tagactatta cactgataat atagtcaatt gaaacaagaa caagacaaaa gagcctcata
    34261 aaaggtattg caacttggta ataccttttt gaggtgcttt ttgatatgag cccatgtttt
    34321 ctcaatagga ttgtactcag gtgagtaggg aggaagaggt aaaagtttat gcctaaactc
    34381 ttcgcataaa agttctaact tacccattct atggaatctt gcattatcca taataataac
    34441 cgatggtgtg gttaatgttg gtaagagaaa cttctgaaac catacttcaa aaaagtcgct
    34501 cgtcatcatc tcttcgtaag tcattggagc gattaactca ccattcattt gttagacctg
    34561 caaccaaaga aatcctccga tatcttcttc cagatacttt gcctcttctt aactgacctt
    34621 ttaaagagcg accatattct cgataaaaat aagtatcgaa tcctgtttca tcaatc
//
LOCUS       DAVLVP010000013        34360 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.013, whole genome shotgun sequence.
ACCESSION   DAVLVP010000013 DAVLVP010000000
VERSION     DAVLVP010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 34360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34360
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.013"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            535..987
                     /locus_tag="VYN69_000607"
     CDS             535..987
                     /locus_tag="VYN69_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1532920.1"
                     /translation="MIITKISRLGTYVGVNPHFATLIDFLEKTGLENLTEGSIAIDGN
                     RLFGNCFTYLADGQAGAFFETHQKYLDIHLVLENEEAMAVTSPENVSVTQEYDEEKDI
                     ELYTGKVEQLVHLRAGECLITFPEDLHQPKVRINDEPVKKVVFKVAIS"
     gene            1006..2343
                     /locus_tag="VYN69_000608"
     CDS             1006..2343
                     /locus_tag="VYN69_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1532921.1"
                     /translation="MKKMRKFLCLAGIALAAVALVACSGKKEATTSTEPPTELSGEIT
                     MWHSFTQGPRLESIQKSADAFMQKHPKTKIKIETFSWNDFYTKWTTGLANGNVPDIST
                     ALPNQVMEMVNSDALVPLNDSIKRIGQDKFNETALNEAKIGDDYYSVPLYSHAQVMWV
                     RTDLLKEHNIEVPKTWDQLYEASKKLKEAGVYGLSVPFGTNDLMATRFLNFYVRSGGG
                     SLLTKDLKADLTSQLAQDGIKYWVKLYKEISPQDSLNFNVLQQATLFYQGKTAFDFNS
                     GFHIGGINANSPQLIDSIDAYPIPKIKESDNDQGIETSNIPMVVWKNSKHPEVAKAFL
                     EALYNEEDYVKFLDSTPVGMLPTIKGISDSAAYKENETRKKFKHAEEVITEAVKKGTA
                     IGYENGPSVQAGMLTNQHIIEQMFQDIITNGTDPMKAAKEAEKQLNDLFEAVQ"
     gene            2422..3306
                     /locus_tag="VYN69_000609"
     CDS             2422..3306
                     /locus_tag="VYN69_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1532922.1"
                     /translation="MVKERNLTRWIFVLPAMIIVGLLFVYPFFSSIFYSFTNKHLIMP
                     NYKFVGLANYKAVLSDPNFFNAFFNSIKWTVFSLVGQVLVGFVLALALHRVRHFKKLY
                     RTLLIVPWAFPTIVIAFSWQWILNGVYGYLPNLIVKLGLMEHTPAFLTDSTWAFLCLV
                     FINIWFGAPMIMVNVLSALQTVPEEQFEAAKIDGASSWQVFKFIVFPHIKVVVGLLVV
                     LRTVWIFNNFDIIYLITGGGPANATTTLPIFAYNLGWGTKLLGRASAVTVLLFIFLVA
                     ICFIYFAIISKWEKEGRK"
     gene            3306..4139
                     /locus_tag="VYN69_000610"
     CDS             3306..4139
                     /locus_tag="VYN69_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1532923.1"
                     /translation="MKKKSSIYLDILSHVLLVGATIVAVFPLVWIIISSVKGKGELTQ
                     YPTRFWPEQFTLDYFTHVINDLHFIDNIRNSLIIALATTLIAIIISAMAAYGIVRFFP
                     KLGAIMSRLLVITYIFPPILLAIPYSIAIAKVGLTNSLFGLMMVYLSFSVPYAVWLLV
                     GFFQTVPIGIEEAARIDGANKFVTFYKVVLPIVAPGIVATAIYTFINAWNEFLYALIL
                     INNTGKMTVAVALRSLNGSEILDWGDMMAASVIVVLPSIIFFSIIQNKIASGLSEGSV
                     K"
     gene            4157..6250
                     /gene="nanB"
                     /locus_tag="VYN69_000611"
     CDS             4157..6250
                     /gene="nanB"
                     /locus_tag="VYN69_000611"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neuraminidase NanB"
                     /protein_id="HEV1532924.1"
                     /translation="MNKRGLYSKLGISVVGISLLMGVPTLIHANELNYGQLSISPIFQ
                     GGSYQLNNKSIDISSLLLDKLSGESQTVVMKFKADKPNSLQALFGLSNSKAGFKNNYF
                     SIFMRDSGEIGVEIRDAQKGINYLFSRPASLWGKHKGQAVENTLVFVSDSKDKTYTMY
                     VNGIEVFSETVDTFLPISNINGIDKATLGAVNREGKEHYLAKGSIDEISLFNKAISDQ
                     EVSNIPLSNPFQLIFQSGDSTQANYFRIPTLYTLSSGRVLSSIDARYGGTHDSKSKIN
                     IATSYSDDNGKTWSEPIFAMKFNDYEEQLVYWPRDNKLKNSQISGSASFIDSSIVEDK
                     KSGKTILLADVMPAGIGNNNANKADSGFKEINGHYYLKLKKNGDNDFRYTVRENGVVY
                     DETTNKPTNYTINDKYEVLEGGKSLTVEQYSVDFDSGSLRERHNGKQVPMNVFYKDSL
                     FKVTPTNYIAMTTSQNRGESWEQFKLLPPFLGEKHNGTYLCPGQGLALKSSNRLIFAT
                     YTNGELIYLISDDSGQTWKKSSASIPFENATAEAQMVELRDGVIRTFFRTTTGKIAYM
                     TSRDSGETWSEVSYIDGIQQTSYGTQVSAIKYSQLIDGKEAVILSTPNSRSGRKGGQL
                     VVGLVNKEDDSIDWKYHYDIDLPSYGYAYSAVTELPNHHIGVLFEKYDSWSRNELHLS
                     NVVQYIDLEINDLTK"
     gene            6262..7365
                     /locus_tag="VYN69_000612"
     CDS             6262..7365
                     /locus_tag="VYN69_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1532925.1"
                     /translation="MVKYGVVGAGYFGAELARYMQKNDGAEITLLYDPDNAEAIAEEL
                     GAKVASSLDELVSSDEVDCVIVATPNNLHKEPVIKAAQHGKNVFCEKPIALSYQDCRE
                     MVDACKENNVTFMAGHIMNFFNGVHHAKELINQGVIGDVLYCHTARNGWEEQQPSVSW
                     KKIREKSGGHLYHHIHELDCVQFLMGGMPETVTMTGGNVAHEGEHFGDEDDMIFVNME
                     FSNKRFALLEWGSAYRWGEHYVLIQGSKGAIRLDLFNCKGTLKLDGQESYFLIHESQE
                     EDDDRTRIYHSTEMDGAIAYGKPGKRTPLWLSSVIDKEMRYLHEIMEGAPVSEEFAKL
                     LTGEAALEAIATADACTQSMFEDRKVKLSEIVK"
     gene            7539..8237
                     /locus_tag="VYN69_000613"
     CDS             7539..8237
                     /locus_tag="VYN69_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEV1532926.1"
                     /translation="MPQISKEALIEQIKDGIIVSCQALPHEPLYTEAGGVIPLLVKAA
                     EQGGAVGIRANSVRDIKEIKEVTKLPIIGIIKRDYPPQEPFITATMKEVDELAELDIE
                     VIALDCTKRERYDGLEIQEFIRQVKEKYPNQLLMADTSIFEEGLAAVEAGIDFVGTTL
                     SGYTSYSPKVDGPDFELIKKLCDAGVDVIAEGKIHTPEQAKQILEYGVRGIVVGGAIT
                     RPKEITERFVASLK"
     gene            8256..9773
                     /locus_tag="VYN69_000614"
     CDS             8256..9773
                     /locus_tag="VYN69_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1532927.1"
                     /translation="MFKVLQKVGKAFMLPIAILPAAGLLLGIGGALSNPTTIATYPIL
                     DNSIFQSIFQVMSSAGEVVFSNLSLLLCVGLCIGLAKRDKGTAALAGVTGYLVMTATI
                     KALVKLFMAEGSAIDTGVIGALVVGIVAVYLHNRYNNIQLPSALGFFGGSRFVPIVTS
                     FSSILIGFVFFVIWPPFQQLLVSTGGYISQAGPIGTFLYGFLMRLSGAVGLHHIIYPM
                     FWYTELGGVETVAGQTVVGAQKIFFAQLADLAHSGLFTEGTRFFAGRFSTMMFGLPAA
                     CLAMYHSVPKNRRKKYAGLFFGVALTSFITGITEPIEFMFLFVSPVLYVVHAFLDGVS
                     FFIADVLNISIGNTFSGGVIDFTLFGILQGNAKTNWVLQIPFGLIWSVLYYIIFRWFI
                     TQFNVLTPGRGEEVDSKEISESADSTSNIADYLKQDSLQIIRALGGSNNIEDVDACVT
                     RLRVAVKEVNQVDKALLKQIGAVDVLEVKGGIQAIYGAKAILYKNSINEILGVDD"
     gene            9898..11226
                     /locus_tag="VYN69_000615"
     CDS             9898..11226
                     /locus_tag="VYN69_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1532928.1"
                     /translation="MKFRKLACTVLAGAAVLGLAACGNSGGSKDAAKSGGDGAKTEIT
                     WWAFPVFTQEKTGDGVGTYEKSIIEAFEKANPDIKVKLETIDFKSGPEKITTAIEAGT
                     APDVLFDAPGRIIQYGKNGKLAELNDLFTDEFVKDVNNENIVQASKAGDKAYMYPISS
                     APFYMAMNKKMLEDAGVANLVKEGWTTDDFEKVLKALKDKGYTPGSLFSSGQGGDQGT
                     RAFISNLYSGSVTDEKVSKYTTDDPKFVKGLEKATSWIKDNLINNGSQFDGGADIQNF
                     ANGQTSYTILWAPAQNGIQAKLLEASKVEVVEVPFPSDEGKPALEYLVNGFAVFNNKD
                     DKKVAASKKFIQFIADDKEWGPKDVVRTGAFPVRTSFGKLYEDKRMETISGWTQYYSP
                     YYNTIDGFAEMRTLWFPMLQSVSNGDEKPADALKAFTEKANETIKKAMKQ"
     gene            11478..12344
                     /locus_tag="VYN69_000616"
     CDS             11478..12344
                     /locus_tag="VYN69_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1532929.1"
                     /translation="MRETVISYAFLAPVLFFFVIFVLAPMVMGFITSFFNYSMTKFEF
                     VGLDNYIRMFKDPVFTKSLINTVILVIGSVPVVVLFSLFVASQTYHQNVIARSFYRFV
                     FFLPVVTGSVAVTVVWKWIYDPLSGILNFVLKSSHIISQNISWLGDKNWALMAIMIIL
                     LTTSVGQPIILYIAAMGNIDNSLVEAARVDGATEFQVFWKIKWPSLLPTTLYIAIITT
                     INSFQCFALIQLLTSGGPNYSTSTLMYYLYEKAFQLTEYGYANTIGVFLAVMIAIVSF
                     VQFKVLGNDVEY"
     gene            12360..13199
                     /locus_tag="VYN69_000617"
     CDS             12360..13199
                     /locus_tag="VYN69_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1532930.1"
                     /translation="MQSTEKKPLTAFTVISTIILLLLTVLFIFPFYWILTGAFKSQPD
                     TIVIPPQWFPKMPTMENFQQLMVQNPALQWMWNSVFISLVTMFLVCATSSLAGYVLAK
                     KRFYGQRILFAIFIAAMALPKQVVLVPLVRIVNFMGIHDTLWAVILPLIGWPFGVFLM
                     KQFSENIPTELLESAKIDGCGEIRTFWSVAFPIVKPGFAALAIFTFINTWNDYFMQLV
                     MLTSRNNLTISLGVATMQAEMATNYGLIMAGAALAAVPIVTVFLVFQKSFTQGITMGA
                     VKG"
     gene            13471..13923
                     /locus_tag="VYN69_000618"
     CDS             13471..13923
                     /locus_tag="VYN69_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000575445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HEV1532931.1"
                     /translation="MIFDDLKNITFYKGIHPNLDKAIDYLYQHRKDSFELGKYEIDGD
                     KVFLVVQENVLNQAENDQFEYHKNYADLHLLVEGHEYSSYGSRIKDEAVAFDEASDIG
                     FVHCHEHYPLLLGYHNFAIFFPGEPHQPNGYAGMEEKVRKYLFKILID"
     gene            14020..14364
                     /locus_tag="VYN69_000619"
     CDS             14020..14364
                     /locus_tag="VYN69_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1532932.1"
                     /translation="MVSSKYAARASFLDGQGITVDEMAWIIRGIVNALIGRYIKLGTY
                     AAKYGISMARSILSRVAATAAARVGLLTKISGWILRVAVNVADVYGNFANNIAAAWDA
                     YDKIPNNGRINF"
     gene            14641..15285
                     /locus_tag="VYN69_000620"
     CDS             14641..15285
                     /locus_tag="VYN69_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEV1532933.1"
                     /translation="MAQKGVSLIKAAFDTDNFLMRFSEKVLDIVTANLLFVVSCLPIV
                     TIGVAKISLYETMFEIKKSRRVPVFKIYLRSFKQNLKLGLQLGLMELGIVFLTLSDLY
                     LFWGQTALPFQLLKAICLGILIFLTIVMLASYPIAARYDLSWKEILQKGLMLASFNFP
                     WFFLMLAILVLIVMVLYLSAFSLLLGGSAFLLFGFGLLAFLQVGVMEKLFAKYE"
     gene            15458..16375
                     /locus_tag="VYN69_000621"
     CDS             15458..16375
                     /locus_tag="VYN69_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HEV1532934.1"
                     /translation="MSDLKKYEGVIPAFYACYDEQGEVSPERTRALVQYFIDKGVQGL
                     YVNGSSGECIYQSVEDRKLILEEVMAVAKGKLTIIAHVACNNTKDSMELARHAESLGV
                     DAIATIPPIYFRLPEYSVAKYWNDISSAAPNTDYVIYNIPQLAGVALTPSLYTEMLKN
                     PRVIGVKNSSMPVQDIQTFVSLGGEDHIVFNGPDEQFLGGRLMGARAGIGGTYGAMPE
                     LFLKLNQLIADKDLETARELQYAINAIIGKLTSAHGNMYGVIKEVLKINEGLNIGSVR
                     SPLTPVTEEDRPVVEAAAALIRETKERFL"
     gene            16393..17277
                     /locus_tag="VYN69_000622"
     CDS             16393..17277
                     /locus_tag="VYN69_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1532935.1"
                     /translation="MTYYVAIDIGGTNIKYGLVDQEGQLLESHEMPTEAHKGGPHILQ
                     KTKDIVASYLEKGPVAGVAISSAGMVDPDKGEIFYAGPQIPNYAGTQFKKEIEESFTI
                     PCEIENDVNCAGLAEAVSGSGKGASVTLCLTIGTGIGGCLIMDRKVFHGFSNSACEVG
                     YMHMQDGAFQDLASTTALVKYVAEAHGEDVDQWNGRRIFKEATEGNKICMEGIDRMVD
                     YLGKGLANICYVANPEVVILGGGIMGQEAILKPKIRTALKEALVPSLAEKTRLEFAHH
                     QNTAGMLGAYYHFKTKQS"
     gene            complement(17293..18144)
                     /locus_tag="VYN69_000623"
     CDS             complement(17293..18144)
                     /locus_tag="VYN69_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEV1532936.1"
                     /translation="MDKPDIATIIDSHFEEMTDLEQEIARYFLQAETITDDLSSQQVT
                     QKLHISQAALTRFAKKCGFTGYREFIFQYQHQAENQANQVSKHSPLTKRVLRSYSNMR
                     EQTQDLIDEIQLERIAQLIEDAERIYFFGTGSSGLVAREMKLRFMRLGVVCEALTDQD
                     GFAWTTSIMDENCLVLGFSLSGSTPSILDSLLDAKEMGAKTVLFTSVPNKNSQTYTET
                     VLVATHSQPSYIQRISAQLPMLFFIDLIYAYFLEINRESKEKIFNSYWENKKLNGYRR
                     QKRVRKS"
     gene            18344..20401
                     /gene="pbp2b"
                     /locus_tag="VYN69_000624"
     CDS             18344..20401
                     /gene="pbp2b"
                     /locus_tag="VYN69_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2B"
                     /protein_id="HEV1532937.1"
                     /translation="MRLICMRKFNSHSIPIRLNLLFSIVILLFMTIIGRLLYMQVLNK
                     DFYEKKLASASQTKITSSSARGEIYDASGKPLVENTLKQVVSFTRSNKMTATDLKETA
                     KKLLTYVSISSPNLTERQLADYYLADPEIYKKIVEALPSEKRLDSDGNRLSESELYNN
                     AVDSVQTSQLNYTEDEKKEIYLFSQLNAVGNFATGTIATDPLNDSQVAVIASISKEMP
                     GISISTSWDRKVLETSLSSIVGSVSSEKAGLPAEEAEAYLKKGYSLNDRVGTSYLEKQ
                     YEETLQGKRSVKEIHLDKYGNMESVDTIEEGSKGNNIKLTIDLAFQDSVDALLKSYFN
                     SELENGGAKYSEGVYAVALNPKTGAVLSMSGIKHDLKTGELTPDSLGTVTNVFVPGSV
                     VKAATISSGWENGVLSGNQTLTDQSIVFQGSAPINSWYTQAYGSFPITAVQALEYSSN
                     TYMVQTALGLMGQTYQPNMFVGTSNLESAMEKLRSTFGEYGLGTATGIDLPDESTGFV
                     PKEYSFANYITNAFGQFDNYTPMQLAQYVATIANNGVRVAPRIVEGIYGNNDKGGLGD
                     LIQQLQPTEMNKVNISDSDMSILHQGFYQVAHGTSELTTGRAFSNGALVSISGKTGTA
                     ESYVADGQQATNTNAVAYAPSDNPQIAVAVVFPHNTNLTNGVGPSIARDIINLYQKYH
                     PMN"
     gene            20412..21008
                     /gene="recR"
                     /locus_tag="VYN69_000625"
     CDS             20412..21008
                     /gene="recR"
                     /locus_tag="VYN69_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEV1532938.1"
                     /translation="MLYPTPIAKLIDSYSKLPGIGIKTATRLAFYTIGMSADDVNEFA
                     KNLLSAKRELTYCSICGRLTDDDPCSICTDPTRDQTTILVLEDSRDVAAMENIQEYHG
                     LYHVLHGLISPMNGISPDDINLKSLMTRLMDSEVSEVIVATNATADGEATSMYLSRLL
                     KPAGIKVTRLARGLAVGADIEYADEVTLLRAIENRTEL"
     gene            21184..22227
                     /locus_tag="VYN69_000626"
     CDS             21184..22227
                     /locus_tag="VYN69_000626"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEV1532939.1"
                     /translation="MKQTIILLYGGRSAEREVSVLSAESVMRAVDYDRFTVKTFFISQ
                     SGDFIKTQEFSHAPGQEDRLMTNETIDWDKKVAPSAIYEEGAVVFPVLHGPMGEDGSV
                     QGFLEVLKMPYVGCNILSSSLAMDKITTKRVLESAGIAQVPYVAIVEGDDVTAKIAEV
                     EEKLAYPVFTKPSNMGSSVGISKSENQEELRQALELAFRYDSRVLVEQGVNAREIEVG
                     LLGNYDVKSTLPGEVVKDVAFYDYDAKYIDNKVTMDIPAKISDDVVAVMRQNAETAFR
                     AIGGLGLSRCDFFYTDKGEIFLNELNTMPGFTQWSMYPLLWDNMGISYPELIERLVDL
                     AKESFDKREAHLI"
     gene            22311..23684
                     /locus_tag="VYN69_000627"
     CDS             22311..23684
                     /locus_tag="VYN69_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEV1532940.1"
                     /translation="MKLTIHEIAQVVGAKNDISIFEDTQLEKAEFDSRLIGTGDLFVP
                     LKGARDGHDFIETAFENGAAVTLSEKEVSNHPYILVDDVLTAFQSLASYYLEKTTVDV
                     FAVTGSNGKTTTKDMLAHLLSTRYKTYKTQGNYNNEIGLPYTVLHMPEGTEKLVLEMG
                     QDHLGDIHLLSELARPKTAIVTLVGEAHLAFFKDRSEIAKGKMQIADGMASNSLLLAP
                     ADSIVEDYLPTDKKIVRFGQGAELEITDLVERKDSLTFKANFLEQALDFPVTGKYNAT
                     NAMIASYVALQEGVSEEQIRLAFQGLELTRNRTEWKKAANGADILSDVYNANPTAMKL
                     ILETFSAIPANEGGKKIAVLADMKELGVQSVQLHNQMILSLSPDVLDTVIFYGEDIAE
                     LAQLASQMFPIGHVYYFKKTEDQDQFEDLVKQVKESLGAHDQILLKGSNSMNLAKLVE
                     SLENEDK"
     gene            23671..24282
                     /locus_tag="VYN69_000628"
     CDS             23671..24282
                     /locus_tag="VYN69_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1532941.1"
                     /translation="MKTSDFVKYLQRMIAITDTGLTFTKDPFDRERYEDLRSLLSEML
                     NQGSDLDAEEVAEVLKPTSAYATPLMDVRAWIVEDEKICLVRGQGEDSWALPGGFGEV
                     GYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQLQSKQYAKFVFECKLLDGQFQEN
                     QEIADLQFFAIDQLPNLSEKRITKEQIEILWQVYQGQREQYLD"
     gene            24373..25077
                     /locus_tag="VYN69_000629"
     CDS             24373..25077
                     /locus_tag="VYN69_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001055242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02206 family membrane protein"
                     /protein_id="HEV1532942.1"
                     /translation="MNLWDIFFTTQATEPPKFDLFWYVSLFTLLALTFYTAHRYREKK
                     VYQRFFQILQTVQLILLYGWYWVNHMPLSESLPFYHCRMAMFVVLLLPGQSKYKQYFA
                     LLGTFGTLAAFVYPVPDAYPFPHITILSFIFGHLALLGNSLVYLLRQYNARLLDVKGI
                     FLMTFALNALIFVVNLVTGGDYGFLTKPPLVGDHGLVANYLLVSIVLVATISLTKKIL
                     EFFLAQEAEKMIAKEA"
     gene            25296..26669
                     /gene="ftsA"
                     /locus_tag="VYN69_000630"
     CDS             25296..26669
                     /gene="ftsA"
                     /locus_tag="VYN69_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEV1532943.1"
                     /translation="MAREGFFTGLDIGTSSVKVLVAEQRNGELNVIGVSNAKSKGVKD
                     GIIVDIDAAATAIKSAISQAEEKAGISIKSVNVGLPGNLLQVEPTQGMIPVTSDTKEI
                     TDQDVENVVKSALTKSMTPDREVITFIPEEFIVDGFQGIRDPRGMMGVRLEMRGLLYT
                     GPRTILHNLRKTVERAGVQVENVIISPLAMVQSVLNEGEREFGATVIDMGAGQTTVAT
                     IRNQELQFTHILQEGGDYVTKDISKVLKTSRKLAEGLKLNYGEAYPPLASKETFQVEV
                     IGEVEAVEVTEAYLSEIISARIKHILEQIKQELDRRRLLDLPGGIVLIGGNAILPGMV
                     ELAQEVFGVRVKLYVLNQVGIRNPAFAHVISLSEFAGQLTEVNLLAQGAIKGENDLSH
                     QPISFGGMLQKTAQFVQSTPVQPAPAPEVEPVAPTEPMADFQQASQNKPKLADRFRGL
                     IGSMFDE"
     gene            26686..27945
                     /gene="ftsZ"
                     /locus_tag="VYN69_000631"
     CDS             26686..27945
                     /gene="ftsZ"
                     /locus_tag="VYN69_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000144288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEV1532944.1"
                     /translation="MTFSFDTAAAQGAVIKVIGVGGGGGNAINRMVDEGVTGVEFIAA
                     NTDVQALSSTKAETVIQLGPKLTRGLGAGGQPEVGRKAAEESEETLTEAISGADMVFI
                     TAGMGGGSGTGAAPVIARIAKDLGALTVGVVTRPFGFEGSKRGQFAVEGINQLREHVD
                     TLLIISNNNLLEIVDKKTPLLEALSEADNVLRQGVQGITDLITNPGLINLDFADVKTV
                     MANKGNALMGIGIGSGEERVVEAARKAIYSPLLETTIDGAEDVIVNVTGGLDLTLIEA
                     EEASQIVNQAAGQGVNIWLGTSIDESMRDEIRVTVVATGVRQDRVEKVVAPQARSATN
                     YRETVKPAHSHGFDRHFDMAETAELPKQNPRRLEPTQASAFGDWDLRRESIVRTTDSV
                     VSPVERFEAPISQDEDELDTPPFFKNR"
     gene            27950..28621
                     /locus_tag="VYN69_000632"
     CDS             27950..28621
                     /locus_tag="VYN69_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEV1532945.1"
                     /translation="MNVKENTELVFREVAEASLSANRECGSVSVIAVTKYVDVPTAEA
                     LLPLGVHHIGENRVDKFLEKYEALKDRDVTWHLIGTLQRRKVKDVIQYVDYFHALDSV
                     KLAGEIQKRSDRVIKCFLQVNISKEESKHGFSREELLEILPELARLDKIEYVGLMTMA
                     PFEASSEQLKEIFKATQDLQREIQEKQIPNMPMTELSMGMSRDYKEAIQFGSTFVRIG
                     TSFFK"
     gene            28631..29170
                     /locus_tag="VYN69_000633"
     CDS             28631..29170
                     /locus_tag="VYN69_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEV1532946.1"
                     /translation="MSLKDRFDRFIDYFTEDEDSSLPYEKRDEPVFTPVNSSQEPALP
                     MNQPSQSAGTKENNITRLHARQQELANQSQRATDKVIIDVRYPRKYEDATEIVDLLAG
                     NESILIDFQYMTEVQARRCLDYLDGACHVLAGNLKKVASTMYLLTPVNVIVNVEDIRL
                     PDEDQQGEFGFDMKRNRVR"
     gene            29170..29433
                     /locus_tag="VYN69_000634"
     CDS             29170..29433
                     /locus_tag="VYN69_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEV1532947.1"
                     /translation="MIFLIRMIYNAVDIYSLILVAFAVMSWFPGAYESSLGRWIVALV
                     KPVLAPLQRLPLQIAGLDLSVWVAIVLVRFLGENLVRFLAMIG"
     gene            29430..30215
                     /locus_tag="VYN69_000635"
     CDS             29430..30215
                     /locus_tag="VYN69_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEV1532948.1"
                     /translation="MNKGIYQHFSIEDRPFLDKGMEWIKKVEDSYAPFLTPFINPHQE
                     KLLKILAKTYGLACSSSGEFVSSEYVRVLLYPDYFQPEFSDFEISLQEIVYSNKFEHL
                     THAKILGTVINQLGIERKLFGDILVDEERAQIMINQQFLLLFQDGLKKIGRIPVSLEE
                     RPFTEKIDKLEQYRELDLSVSSFRLDVLLSNVLKLSRNQANQLIEKKLVQVNYHVVDK
                     SDYTVQVGDLISVRKFGRLRLLQDKGQTKKEKKKITVQLLLSK"
     gene            30224..31012
                     /gene="divIVA"
                     /locus_tag="VYN69_000636"
     CDS             30224..31012
                     /gene="divIVA"
                     /locus_tag="VYN69_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIVA"
                     /protein_id="HEV1532949.1"
                     /translation="MPITSLEIKDKTFGTRFRGFDPEEVDEFLDIVVRDYEDLVRANH
                     DKNLRIKSLEERLSYFDEIKDSLSQSVLIAQDTAERVKQAAHERSNNIIHQAEQDAQR
                     LLEEAKYKANEILRQATDNAKKVAVETEELKNKSRVFHQRLKSTIESQLAIVESSDWE
                     DILRPTATYLQTSDEAFKEVVSEVLGEPIPAPIEEEPIDMTRQFSQAEMEELQARIEV
                     ADKELSEFEAQIKQEVETPTPVVSPQVEEEPLLIQLAQCMKNQK"
     gene            31360..34152
                     /locus_tag="VYN69_000637"
     CDS             31360..34152
                     /locus_tag="VYN69_000637"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEV1532950.1"
                     /translation="MKLKDTLNLGKTAFPMRAGLPTKEPVWQKEWEDAKLYQRRQELN
                     QGKPHFTLHDGPPYANGNIHVGHAMNKISKDIIVRSKSMSGFYAPFIPGWDTHGLPIE
                     QVLSKQGVKRKEMDLVEYLKLCREYALSQVDKQREDFKRLGVSGDWENPYVTLTPDYE
                     AAQIRVFGEMANKGYIYRGAKPVYWSWSSESALAEAEIEYHDLVSTSLYYANKVKDGK
                     GVLDTDTYIVVWTTTPFTITASRGLTVGADIDYVLVQPAGEARKFVVAAELLTSLSEK
                     FGWADVQVLETYRGQELNHIVTEHPWDTAVEELVILGDHVTTDSGTGIVHTAPGFGED
                     DYNVGIANNLEVAVTVDERGIMMKNAGPEFEGQFYEKVVPTVIEKLGNLLLAQEEISH
                     SYPFDWRTKKPIIWRAVPQWFASVSKFRQEILDEIEKVKFHSEWGKVRLYNMIRDRGD
                     WVISRQRAWGVPLPIFYAEDGTAIMVAETIEHVAQLFEEHGSSIWWERDAKDLLPEGF
                     THPGSPNGEFKKETDIMDVWFDSGSSWNGVVVNRPELTYPADLYLEGSDQYRGWFNSS
                     LITSVANHGVAPYKQILSQGFALDGKGEKMSKSLGNTIAPSDVEKQFGAEILRLWVTS
                     VDSSNDVRISMDILSQVSETYRKIRNTLRFLIANTSDFNPAQDTVAYDELRSVDKYMT
                     IRFNQLVKTIRDAYADFEFLTIYKALVNFINVDLSAFYLDFAKDVVYIEGAKSLERRQ
                     MQTVFYDILVKITKLLTPILPHTAEEIWSYLEFETEDFVQLSELPEVQTFANQEEILD
                     TWAAFMDFRGQAQKALEEARNAKVIGKSLEAHLTVYPNEVVKTLLEAVNSNVAQLLIV
                     SELTIAEGPAPEAALSFEDVAFTVERAAGEVCDRCRRIDPTTAERSYQAVICDHCVSI
                     VEENFAEAVAEGFEEK"
BASE COUNT        10548 a         6052 c         7494 g        10266 t
ORIGIN      
        1 tatagagtat ttctctctta aaagtttttg gtgaaacgag atgtagaaag gagatttagc
       61 caaagagtct attagtgcta gaataataga ttagaattat tttagaaaaa cgaagtgagc
      121 agcttataaa ttcaagtccc caaatagatt catactagta tcttttgcaa aaaataaagg
      181 gcgacttcct tcatgaatat caatttcatc tataaggaag gtagctaatt gaactaactt
      241 atttattctg tttgtcgcta gaaaaatcag acctccttgt gaagattgag gagatactta
      301 atgaaaatca aagaagaaac tagcaagcta gtagcagatt gcccaaaaca ccgctttgag
      361 gttgtagata agactgacgt atataatcca aggtgaagcg actgtggttt gaagagattt
      421 tcaaagagta taggctagag agtagtgttt ttatgtcctt ctagtagaaa atgctagaca
      481 gaagaatggg gaacttggat agtaaaaata gattgagaaa ggaggttaga agagatgatt
      541 attacaaaaa ttagccgttt aggaacttat gtgggagtca atccacattt tgcaacatta
      601 atagattttc tagaaaaaac aggactagaa aatttaacag aaggttcgat tgctatcgat
      661 ggtaatcgat tgtttgggaa ttgctttact tatctagcag atggtcaagc aggggctttc
      721 tttgaaacac accaaaaata tttggatatt catttagttt tggaaaacga agaagccatg
      781 gctgttacat cgccggaaaa tgtaagcgtt acccaagaat atgatgaaga gaaagatatt
      841 gaattatata cagggaaagt ggaacagttg gttcatttga gagctggcga atgcctcatc
      901 acttttccag aagatttaca tcaacccaag gttcgtataa atgatgaacc tgtgaaaaaa
      961 gttgtcttta aagttgcgat ttcttaatgt agaaagagaa gaacgatgaa aaaaatgaga
     1021 aagtttttat gtctagctgg aattgcgcta gcggctgttg ccttggtagc ttgttcagga
     1081 aaaaaagaag ctacaactag tactgaacca ccaacagaat tatctggtga gattacaatg
     1141 tggcactcct ttactcaagg accccgttta gaaagtattc aaaaatcagc agatgctttc
     1201 atgcaaaagc atccaaaaac gaaaatcaag attgaaacat tttcttggaa tgacttctat
     1261 actaaatgga ctacaggttt agcaaatgga aatgtgccag atatcagtac agctcttcct
     1321 aaccaagtaa tggaaatggt caactcagat gctttggtgc cgctaaatga ttctatcaag
     1381 cgtattggac aagataaatt taacgaaact gccttaaatg aagcaaaaat cggagatgat
     1441 tactactctg ttcctcttta ttcacatgca caagtcatgt gggttagaac agatttgtta
     1501 aaagaacata atattgaggt tcctaaaact tgggatcaac tctatgaagc ttctaaaaaa
     1561 ttgaaagaag ctggagttta tggcttgtct gttccgtttg gaacaaatga cttaatggca
     1621 acacgtttct tgaacttcta cgtacgtagt ggtggaggaa gcctcttaac aaaagatctt
     1681 aaagcagact tgacaagcca acttgctcaa gatggtatta aatactgggt taaattgtat
     1741 aaagaaatct cacctcaaga ttctttgaac tttaatgtcc ttcaacaagc taccttgttc
     1801 tatcaaggaa aaacagcatt tgactttaac tctggcttcc atatcggagg aattaatgcc
     1861 aacagtcctc aattgattga ttcgattgat gcttatccta ttccaaaaat caaagagtct
     1921 gataacgacc aaggaattga aacctcaaac attccaatgg ttgtttggaa aaattcaaaa
     1981 catccagaag ttgctaaagc attcttagaa gcactttata atgaagaaga ctacgttaaa
     2041 ttccttgatt caactccagt aggtatgttg ccaactatta aggggattag cgattctgca
     2101 gcctataaag aaaatgaaac tcgtaagaag tttaaacatg ctgaagaagt aattactgaa
     2161 gctgttaaaa aaggtactgc tattggttat gaaaatggac caagtgtaca agctggtatg
     2221 ttgactaacc aacacattat tgaacaaatg ttccaagata tcattacaaa tggaacagat
     2281 cctatgaaag cagcaaaaga agcagaaaaa caattaaacg atttatttga ggctgttcag
     2341 tagatgtaaa agactagaaa ataggtgggc tagtgagctg aaaagctcta gcccaatctt
     2401 gtaaaagaag ggagaaggag aatggttaaa gaacgtaatt taactcgctg gatatttgtt
     2461 ttgccagcta tgattatcgt aggattactc tttgtttatc cgtttttctc gagtattttt
     2521 tatagcttta ccaataagca tttgattatg cctaattata aatttgttgg tttggctaac
     2581 tataaagctg tgctatcaga tcccaacttc tttaatgcgt tctttaattc aattaagtgg
     2641 accgttttct cattagttgg tcaagtttta gtagggtttg tattggcttt agctcttcac
     2701 agagtacgcc acttcaagaa attatatagg accttattga ttgttccttg ggcatttcct
     2761 accatcgtca ttgccttttc ttggcagtgg attctaaacg gggtttatgg ctacttacct
     2821 aatctaatcg taaaattagg tttaatggaa catacacctg catttttgac agatagtaca
     2881 tgggcattcc tatgtttggt gtttatcaac atttggtttg gagcaccaat gattatggtt
     2941 aatgtgcttt cagctttgca aacagtacca gaagaacaat ttgaggctgc taagatagat
     3001 ggtgcttcaa gttggcaggt gttcaagttt atcgtctttc cacatattaa agtggttgta
     3061 ggacttctag ttgttttgag aactgtatgg atctttaata actttgacat tatctacctc
     3121 attactggtg gtggaccagc caatgctaca acgacgcttc caatttttgc ttacaacctg
     3181 ggctggggaa ctaaattgtt gggtcgtgct tcagcagtta cagtattgct ctttatcttc
     3241 ttggtggcga tttgctttat ctactttgct atcatcagta agtgggaaaa ggagggtaga
     3301 aaataatgaa gaagaaatcc agtatttatt tagatattct ctcacatgta cttttagttg
     3361 gtgcgaccat cgttgcagtt ttcccattgg tatggattat catatcttct gtcaaaggga
     3421 aaggggaatt aactcagtat ccaacacgat tttggcctga acagtttaca ttagattatt
     3481 tcactcatgt tatcaacgat ttgcacttca ttgataacat tcgaaacagt ttaatcattg
     3541 ccttggctac aacccttatt gcgattatta tttctgctat ggcagcctat ggtattgttc
     3601 gattctttcc taaattggga gcaatcatgt cgagactact cgtcattacc tacattttcc
     3661 caccaatttt gttagcaatt ccctattcaa ttgccattgc taaagttggg ttaacaaata
     3721 gtttatttgg cttgatgatg gtttatctat cttttagtgt tccatatgca gtttggctct
     3781 tagttggatt tttccaaaca gttccaattg gaattgaaga agcggctaga attgatggtg
     3841 caaataaatt tgttacgttt tataaagttg tgctaccgat tgtagcacca ggtattgtag
     3901 caacagctat ttatacattt atcaatgctt ggaatgaatt cctgtatgcc ttgattttga
     3961 ttaacaatac aggaaagatg acagtagcag tagcccttcg ttcacttaat ggttcagaaa
     4021 tactagactg gggagatatg atggcagcgt ctgttattgt agttcttcca tcaattattt
     4081 tcttctctat catccaaaat aagattgcaa gtggattatc agaaggatct gtgaagtaga
     4141 cgaaagaagg aaaaaaatga ataaaagagg tctttattca aaactaggaa tttctgttgt
     4201 aggcattagt cttttaatgg gagttcccac tttgattcat gcgaatgaat taaactatgg
     4261 tcaactgtcc atatctccta tttttcaagg aggttcatat caactgaaca ataagagtat
     4321 agatatcagc tctttgttat tagataaatt gtctggagag agtcagacag tagtaatgaa
     4381 atttaaagca gataaaccaa actctcttca agctttgttt ggcctatcta atagtaaagc
     4441 aggctttaaa aataattact tttcaatttt catgagagat tctggtgaga taggtgtaga
     4501 aataagagac gcccaaaagg gaataaatta tttattttct agaccagctt cattatgggg
     4561 aaagcataaa ggacaggcag ttgaaaatac actagtattt gtatctgatt ctaaagataa
     4621 aacatacaca atgtatgtta atggaataga agtgttctct gaaacagttg atacattttt
     4681 gccaatttca aatataaatg gtatagataa ggcaacacta ggagctgtta atcgtgaagg
     4741 caaggaacat tacctcgcaa aaggaagtat tgatgaaatc agtctattta acaaagcaat
     4801 tagtgatcag gaagtttcaa atattccctt gtcaaatcca tttcagttaa ttttccaatc
     4861 aggagattct actcaagcta actattttag aataccgaca ctatatacat taagtagtgg
     4921 aagagttcta tcaagtattg atgcacgtta tggtgggact catgattcta aaagtaagat
     4981 taatattgcc acttcttata gtgatgataa tgggaaaacg tggagtgagc caatttttgc
     5041 tatgaagttt aatgactatg aggagcagtt agtttactgg ccacgagata ataaattaaa
     5101 gaatagtcaa attagtggaa gtgcttcatt catagattca tccattgttg aagataaaaa
     5161 atctgggaaa acgatattac tagctgatgt tatgcctgcg ggtattggaa ataataatgc
     5221 aaataaagcc gactcaggtt ttaaagaaat aaatggtcat tattatttaa aactaaagaa
     5281 gaatggagat aacgatttcc gttatacagt tagagaaaat ggtgtcgttt atgatgaaac
     5341 aactaataaa cctacaaatt atactataaa tgataagtat gaagttttgg agggaggaaa
     5401 gtctttaaca gtcgaacaat attcggttga ttttgatagt ggctctttaa gagaaaggca
     5461 taatggaaaa caggttccta tgaatgtttt ctataaagat tcgttattta aagtgactcc
     5521 tactaattat atagcaatga caactagtca gaatagagga gagagttggg aacaatttaa
     5581 gttgttgcct ccgttcttag gagaaaaaca taatggaact tacttgtgtc ccggacaagg
     5641 tttagcatta aaatcaagta acagattgat ttttgcaaca tatactaatg gagaactaat
     5701 ctatctcatt tctgatgata gtggtcaaac atggaagaaa tcctcagctt caattccgtt
     5761 tgaaaatgca acagcagaag cacaaatggt tgaactgaga gatggtgtga ttagaacatt
     5821 ctttagaacc actacaggta agatagctta tatgactagt agagattctg gagaaacatg
     5881 gtcggaagtt tcgtatattg atggaatcca acaaacttca tatggcacac aagtatctgc
     5941 aattaaatac tctcaattaa ttgatggaaa agaagcagtc attttgagta caccaaattc
     6001 tagaagtggc cgtaagggag gccaattagt tgtcggttta gtcaataaag aagatgatag
     6061 tattgattgg aaataccact atgatattga tttgccttcg tatggttatg cctattctgc
     6121 ggttacagaa ttgccaaatc atcacatagg tgtgctgttt gaaaaatatg attcgtggtc
     6181 gagaaatgaa ttgcatttaa gcaatgtagt tcagtatata gatttggaaa ttaatgattt
     6241 aacaaaataa aggagaaaaa catggttaaa tacggtgttg ttggagcagg gtattttgga
     6301 gctgaattgg ctcgctacat gcaaaagaat gatggagcag agattactct tctctatgat
     6361 ccagataatg cagaggcgat tgcagaagaa ttgggagcaa aagtagcaag ttccttagat
     6421 gagttggttt ctagcgatga ggtagattgt gttatcgtcg caactccaaa taatcttcat
     6481 aaggaaccgg ttattaaggc tgcacagcat ggtaaaaatg ttttctgtga aaaaccaatt
     6541 gcgctttctt atcaagattg tcgcgagatg gtagatgcgt gtaaagaaaa caatgtaacc
     6601 tttatggcag gacatattat gaatttcttt aatggtgttc atcatgcaaa agaactcatt
     6661 aatcaaggag ttatcggaga cgttctatat tgtcatacag ctcgtaatgg ttgggaagaa
     6721 caacaaccgt cagtatcatg gaaaaaaatt cgtgaaaaat caggtggtca cttgtatcac
     6781 cacatccatg aattggattg cgttcaattc cttatggggg gcatgcctga aactgtaacc
     6841 atgacaggtg gaaatgtggc ccatgaaggt gaacatttcg gtgatgaaga tgatatgatt
     6901 tttgtcaata tggaattttc taataagcgt tttgctttgt tagaatgggg ttcagcttat
     6961 cgttggggtg aacattatgt cttaatccaa ggaagcaaag gtgccatccg cttagactta
     7021 ttcaactgta aaggaactct taagctagat gggcaagaaa gctatttctt gattcacgaa
     7081 tcgcaagaag aagatgatga tcggactcgt atctatcata gtacagagat ggatggagca
     7141 attgcttatg gtaaaccagg taaacgtact ccattatggc tatcatctgt cattgataaa
     7201 gaaatgcgct atctgcatga gattatggaa ggagctccag tatcagaaga atttgcaaaa
     7261 cttttgacag gtgaagctgc cctagaagca attgctactg cagatgcttg tacccagtct
     7321 atgtttgaag atcgcaaagt aaaattgtca gaaattgtaa aataaatttt ggtattctcc
     7381 tatttatagg tcgacttgct cctctgaaag tacttttaga ggagctgttt gactttgcta
     7441 gtttttgaaa ctgaaatcta ttatactaca aactattgaa agcgttttaa ttttaaggta
     7501 taataatctc atagaaataa agaaaaggag gaaagaggat gccacagatt agcaaagaag
     7561 ccttgattga gcaaatcaaa gatggaatca tcgtttcttg tcaggctctt cctcatgaac
     7621 cgctttatac agaagcggga ggggtgattc ccttgctggt caaagcggct gagcaaggtg
     7681 gagcagtcgg tatccgagca aacagtgttc gcgatatcaa ggaaattaag gaagtcacta
     7741 aacttccaat cattgggatt atcaaacgtg attatccacc tcaggaaccc ttcatcacgg
     7801 ctactatgaa agaagttgat gaattggcag aactggacat cgaggtgatt gctctggatt
     7861 gtaccaagcg tgaacgctac gatggtttgg aaattcaaga gttcattcgt caggttaagg
     7921 agaaatatcc taatcagctt ttgatggctg atactagtat cttcgaagaa gggctagcag
     7981 ctgtagaagc aggaattgac tttgtcggaa caaccttatc aggctacaca tcctacagtc
     8041 caaaagtaga cggtccagat tttgaattga ttaagaaact ctgtgatgcc ggtgtagatg
     8101 tcattgcaga aggaaaaatt catacaccag aacaagccaa acaaatcctt gaatatggag
     8161 tgcgaggcat cgttgttggt ggcgccatta ctagaccaaa agagattaca gaacgcttcg
     8221 ttgctagtct taaataagat gtgaggggga gttttatgtt taaagtttta caaaaagttg
     8281 gaaaagcttt tatgttacct atagctatac ttcctgcagc aggtctactt ttggggattg
     8341 gtggtgcact ttcaaaccca accacgatag caacttatcc aatactagac aatagtattt
     8401 ttcaatcaat attccaagta atgagctctg caggagaggt tgtattcagt aatttgtcac
     8461 tacttctctg tgtgggatta tgtattggct tagcgaaacg agataaagga accgctgcgt
     8521 tagcaggagt aactggttac ttagttatga ctgcaacgat caaagcttta gtaaaacttt
     8581 ttatggcaga aggatctgca attgatactg gagttattgg agcattagtt gtcggaatag
     8641 ttgccgtata tttgcacaac cgatataaca atattcaatt accttccgct ttaggattct
     8701 ttggaggttc acgcttcgtt cctattgtta catcgttctc ttctatcttg attggctttg
     8761 tcttctttgt tatttggcca cctttccaac aacttcttgt ttctacaggt ggatatattt
     8821 ctcaggcggg tccaattgga acttttctat atggattttt aatgagactt tctggagcag
     8881 taggcttaca tcatataatt taccctatgt tttggtatac tgaacttggt ggtgttgaaa
     8941 ctgttgcagg acaaacagtg gttggagctc aaaaaatatt ttttgctcaa ttagccgatt
     9001 tggcccattc tggattattt acagaaggaa caaggttttt tgcaggtcgt ttctcaacaa
     9061 tgatgttcgg tttaccggct gcctgtttag cgatgtacca tagtgttcct aaaaatcgtc
     9121 gtaaaaaata cgcgggtttg ttttttggag ttgctttaac atcttttatt accggtatta
     9181 cagaaccaat tgaatttatg tttctattcg tcagtccggt tctatatgtt gttcacgcat
     9241 tccttgatgg tgttagcttc tttattgcag acgtcttaaa tatttcaata ggaaacacat
     9301 tttcaggagg tgtaatcgat ttcactttat ttggaatttt gcaggggaac gctaagacga
     9361 attgggttct tcagattcca tttggactta tttggagtgt tttgtattat attattttta
     9421 gatggttcat tactcaattc aacgttctaa cgccaggacg aggagaagaa gtagattcta
     9481 aagaaatttc tgaatccgca gattcaactt caaatattgc agattattta aaacaggata
     9541 gcctacaaat tatcagagcc ttgggtggat caaataatat agaagatgta gatgcttgtg
     9601 tgacacgttt acgtgtagct gtaaaagaag ttaatcaagt tgataaagca cttttaaaac
     9661 aaattggtgc agttgatgtc ttagaagtga agggtggcat tcaagcaatc tatggagcaa
     9721 aagcaatctt atataaaaat agtattaatg aaattttagg tgtagatgat taagtactta
     9781 ctgacttaat aaaaaacaga ggagagtgat ggatgagtag gatgaaatga aatcgcatac
     9841 aagaaataaa gaactcatta tccaagttgg atacgcttat tacataggag aatacaaatg
     9901 aaatttagaa aattagcttg tacagtactt gcgggtgctg cggttcttgg tcttgctgct
     9961 tgtggcaatt ctggcggaag taaagatgct gccaaatcag gtggtgacgg tgccaaaaca
    10021 gaaatcactt ggtgggcatt cccagtattt acccaagaaa aaactggtga cggtgttgga
    10081 acttatgaaa aatcaatcat cgaagcgttt gaaaaagcaa acccagatat aaaagtgaaa
    10141 ttggaaacca tcgacttcaa gtcaggtcct gaaaaaatca caacagccat cgaagcagga
    10201 acagctccag acgtactctt tgatgcacca ggacgtatca tccaatacgg taaaaacggt
    10261 aaattggctg agttgaacga cctcttcaca gatgaatttg ttaaagatgt caacaatgaa
    10321 aacatcgtac aagcaagtaa agctggagac aaggcttata tgtatccgat tagttctgcc
    10381 ccattctaca tggcaatgaa caagaaaatg ttagaagatg ctggagtagc aaaccttgta
    10441 aaagaaggtt ggacaactga tgattttgaa aaagtattga aagcacttaa agacaagggt
    10501 tacacaccag gttcattgtt cagttctggt caagggggag accaaggaac acgtgccttt
    10561 atctctaacc tttatagcgg ttctgtaaca gatgaaaaag ttagcaaata tacaactgat
    10621 gatcctaaat tcgtcaaagg tcttgaaaaa gcaactagct ggattaaaga caatttgatc
    10681 aataatggtt cacaatttga cggtggggca gatatccaaa actttgccaa cggtcaaaca
    10741 tcttacacaa tcctttgggc accagctcaa aatggtatcc aagctaaact tttagaagca
    10801 agtaaggtag aagtggtaga agtaccattc ccatcagacg aaggtaagcc agctcttgag
    10861 taccttgtaa acgggtttgc agtattcaac aataaagacg acaagaaagt cgctgcatct
    10921 aagaaattca tccagtttat cgcagatgac aaagagtggg gacctaaaga cgtagttcgt
    10981 acaggtgctt tcccagtccg tacttcattt ggaaaacttt atgaagacaa acgcatggaa
    11041 acaatcagcg gctggactca atactactca ccatactaca acactattga tggatttgct
    11101 gaaatgagaa cactttggtt cccaatgttg caatctgtat caaatggtga cgaaaaacca
    11161 gcagatgctt tgaaagcctt cactgaaaaa gcgaacgaaa caatcaaaaa agctatgaaa
    11221 caatagtcct tagttattct ataaaaagta gttttttaaa gaacctaaga gtgtataccc
    11281 ccttttccct ctacacagat agtgtaagaa aagggggctt ttgtttaaaa tgtaagaaac
    11341 tgtcacgaaa ttaaaatgaa gttcttacat aagcgaacca taaaaaattt cattttgatt
    11401 ttaaaacagt tcaagaaagt caaaaaatta ttctatttga aagagaggtg ccgactgtga
    11461 aagtcaataa aatccgtatg cgggaaacag tgatttccta cgctttccta gcaccagtat
    11521 tattcttctt tgtcatcttt gtgttggctc cgatggtgat gggcttcatt acaagtttct
    11581 ttaactactc aatgactaaa tttgagtttg tgggcttaga taactatatc cgtatgttta
    11641 aagatcctgt ctttacaaaa tctctgatta acacagttat tttggttatt ggatctgtac
    11701 cagttgttgt tctattctca ctctttgtag catctcagac ctatcatcaa aatgtcattg
    11761 ccagatcctt ctaccgtttc gtcttcttcc ttcctgttgt aacgggtagt gttgccgtga
    11821 cagttgtttg gaaatggatt tatgacccgc tatcagggat tctaaacttt gtccttaagt
    11881 caagccacat catcagccaa aacatttctt ggttgggaga taaaaactgg gcattgatgg
    11941 cgattatgat tattctcttg accacttcag ttggtcagcc catcatcctt tatatcgctg
    12001 ccatggggaa tattgacaat tcactggttg aagcggcgcg tgttgatggc gcaactgagt
    12061 ttcaagtttt ttggaagatt aaatggccaa gccttcttcc aacaactctt tatattgcaa
    12121 tcatcacaac aattaactca ttccagtgtt tcgccttgat tcagcttttg acatctggtg
    12181 gtccaaacta ctcaacaagt accttgatgt actaccttta cgaaaaagcc ttccaattga
    12241 cagaatacgg ctatgccaac acaattggtg tcttcttggc agtcatgatt gctatcgtaa
    12301 gctttgttca atttaaagta cttggaaacg acgtagaata ctaaagaaag gagacagcta
    12361 tgcaatctac agaaaaaaaa ccattaacag cctttactgt tatttcaaca atcattttgc
    12421 tcttgttgac tgtgctgttc atctttccat tctactggat tttgacaggg gcattcaaat
    12481 cacaacctga tacaattgtt attcctcctc agtggttccc taaaatgcca accatggaaa
    12541 acttccaaca actcatggtg cagaaccctg ccttgcaatg gatgtggaac tcagtattta
    12601 tctcattggt aaccatgttc ttagtttgtg caacctcatc tctagcaggt tatgtattgg
    12661 ctaaaaaacg tttctatggt caacgcattc tatttgctat ctttatcgct gctatggcgc
    12721 ttccaaaaca agttgtcctt gtaccattgg tacgtatcgt caacttcatg ggaatccacg
    12781 atactctctg ggcagttatc ttgcctttga ttggatggcc attcggtgtc ttcctcatga
    12841 aacaatttag tgaaaatatt cctacagagt tgcttgaatc agctaaaatc gacggttgtg
    12901 gtgagattcg taccttctgg agtgtagcct tcccgattgt gaaaccaggg tttgcagccc
    12961 ttgcaatctt taccttcatc aatacttgga atgactactt catgcaattg gtaatgttga
    13021 cttcacgtaa caatttgacc atctcacttg gggttgcgac catgcaggct gaaatggcaa
    13081 ccaactatgg tttgattatg gcaggagctg cccttgctgc tgttccaatc gtcacagtct
    13141 tccttgtctt ccaaaaatcc ttcacacagg gtattactat gggagcggtc aaaggataat
    13201 actctgcgaa aatctcttca aactacgtca gcttcacctt gccatactta agtattgcct
    13261 gcggttagct tcctagtttg ttcttcaatt ttcattgagt ataggaaaat caatctatca
    13321 agatacagaa gtatatttta tagatttaga gaatatagag gttataagtg tctacaaaat
    13381 ggagggtatg cagttacttt atgaagtttt gtcagacact tataaactta agaatggttt
    13441 tagttaacta tcagaaacga aggaaagagt atgatttttg acgatttgaa aaacatcacc
    13501 ttttacaaag ggattcatcc taatttagac aaggctatcg actatctcta ccaacatcgt
    13561 aaggattctt tcgaattagg aaagtatgag attgatggag ataaagtctt tctagttgtt
    13621 caggaaaatg tcctcaatca agctgaaaat gatcaatttg agtatcataa gaactatgca
    13681 gatttgcatt tgctggtaga aggacatgaa tattcgagct acggttcacg tatcaaagac
    13741 gaggcagtag cattcgacga agcgagtgac attggctttg ttcattgtca tgaacactac
    13801 ccactcttgt tgggttatca caattttgcg attttcttcc caggtgagcc acatcagcca
    13861 aatggttatg caggcatgga agaaaaggtt cgaaaatatc tctttaaaat tttgattgat
    13921 taaaaatagg atgaattgtt tttttgtaaa gctttgataa tactctacca tgaaattgat
    13981 ctttgtgagg tagagaaatg agaataaaat atttaaaaat tggtatcttc taagtatgct
    14041 gcaagagcta gtttcttaga tggacagggg attacagttg atgagatggc ttggataatt
    14101 aggggcattg tgaatgcatt gattggtaga tacataaaat taggtactta tgcggctaag
    14161 tatggtatta gtatggcacg ctcgatctta agtagggtag ctgcaactgc agcagcaaga
    14221 gtaggattac tgaccaagat ttctggatgg attttacgag tagctgtgaa tgtagctgat
    14281 gtatatggta attttgccaa caatattgct gcagcttggg atgcatatga taaaattcct
    14341 aacaatggtc gtataaactt ttaaaatgcg agaatgaaag cactttgtat ttttttattg
    14401 aatatgttag cttggacagt gcttgcaatg ataattcgtg gagggctaga tggatttgat
    14461 aggcatactt ggagtactat tttaattgct tcgctgttcg gggtatatga ttataagccc
    14521 atagataaaa atagaaaaaa gtccaaaaga aaaaatagat ttgttcatgg tagggactta
    14581 tgaaagcttt actgacaaaa aagaaaacag tttacaaaga aaaatgatgg aggagcaaac
    14641 atggcacaaa aaggagtaag ccttatcaag gcagcatttg atacagacaa ctttctcatg
    14701 cgttttagtg agaaagtctt ggacatcgtg acagccaatc ttctttttgt cgtctcttgt
    14761 ttgcccatcg tgacgattgg agtggctaaa atcagcctct acgagaccat gtttgagatt
    14821 aagaagagca gacgggtgcc tgtttttaaa atctatctaa gatctttcaa gcaaaatctg
    14881 aaactaggtc ttcagctggg tttaatggag ttaggaattg tgtttcttac cctttcagat
    14941 ctctatcttt tctggggtca aacagctctg cccttccaat tgctgaaagc catttgttta
    15001 ggtattctga tttttcttac tatcgtgatg ctggctagtt accctatcgc ggcacgttat
    15061 gacctatctt ggaaagaaat tcttcaaaaa ggattgatgt tggctagttt taactttcct
    15121 tggttcttcc tcatgttagc cattcttgtc ctcattgtga tggttcttta tctgtctgcc
    15181 ttcagtctac tcttaggtgg ctcagccttc ctactttttg ggtttggact tcttgctttt
    15241 ttacaagtgg gagtgatgga aaagctattt gctaagtatg agtaaggggt gcaattattt
    15301 ctgaaactac tttcaaaggc tccaaacgct attctataag cgagaaacta aaatctaagt
    15361 gtctataaga tattgaaagc cttttcggta aagtgtatac taaagaagta aagaaaaatc
    15421 tgctttagca gaaaataaaa ataataggag aaaatctatg tcagatttga aaaaatacga
    15481 aggtgtcatt ccagccttct acgcatgtta tgatgagcaa ggagaagtaa gtccagagcg
    15541 tacgcgtgcc ttggttcaat acttcattga taaaggtgtt caaggtcttt atgtcaatgg
    15601 ttcttctggt gaatgtatct accaaagcgt tgaagatcgc aagttgattt tggaagaagt
    15661 catggcggta gccaaaggta aattgaccat tattgcccat gttgcttgca ataatactaa
    15721 agatagtatg gaacttgctc gccatgctga aagcttggga gtagatgcta ttgcaacgat
    15781 tccaccaatt tacttccgct tgccagaata ctcagttgcc aaatactgga acgatatcag
    15841 ttctgcagct ccaaacacag actacgtgat ttacaacatt cctcaattgg caggggttgc
    15901 tttgactcca agcctttaca cagaaatgtt gaaaaatcct cgtgttatcg gtgttaagaa
    15961 ctcttctatg ccagttcaag atatccaaac ctttgtcagc cttggtgggg aagaccatat
    16021 cgtctttaat ggtcctgatg agcagttcct aggaggacgc ctcatgggtg ctagggctgg
    16081 tatcggtggt acttatggtg ctatgccaga actcttcttg aaactcaatc agttgattgc
    16141 ggataaggac ctagaaacag cgcgtgaatt gcagtatgct atcaacgcaa tcattggtaa
    16201 actcacttct gctcatggaa atatgtacgg tgtcattaaa gaagtcttga aaatcaatga
    16261 aggcttgaat attggatctg ttcgttcacc attgacacca gtgactgaag aagatcgtcc
    16321 agttgtagaa gcggctgctg ccttgattcg tgaaaccaag gagcgcttcc tctaatctaa
    16381 aaggaggtat ttatgacata ctacgttgca attgatatcg gtggaaccaa catcaagtat
    16441 ggtttggttg atcaagaggg gcaacttctt gaatcgcatg aaatgccaac tgaggcgcat
    16501 aagggtggac ctcatatctt acaaaagacc aaagatatcg tagctagtta tttagaaaaa
    16561 ggcccagtag caggtgttgc catatcttct gctgggatgg tggatccgga taagggtgag
    16621 attttctatg ctgggcctca aatccctaac tacgcaggca cccagttcaa aaaggaaatc
    16681 gaagaaagct ttactattcc ttgtgagatt gaaaatgatg tcaactgtgc aggtcttgct
    16741 gaggcagtat ctggttcagg caagggagca agtgtgacac tttgcttgac cattggaacc
    16801 ggtatcggtg gttgcttgat tatggatagg aaagtcttcc atggttttag caattcagcc
    16861 tgtgaagtcg ggtatatgca tatgcaggat ggagcttttc aggatctcgc ttctacaaca
    16921 gctttagtga aatatgtagc tgaagcccat ggagaagatg ttgatcagtg gaatggccga
    16981 cgtatcttta aggaagctac tgaaggaaac aaaatctgca tggaaggtat tgaccgtatg
    17041 gttgactatc taggaaaagg tctggcaaat atttgttatg tggccaatcc agaagtggtt
    17101 attcttggtg gtggtatcat ggggcaagag gctatcctca aacctaagat ccgcacagcc
    17161 ttgaaagagg cattggtacc aagtttagca gaaaaaacac gattagaatt tgcccatcac
    17221 caaaatacag cagggatgtt gggtgcatat tatcatttta agacaaaaca atcctagttt
    17281 ggctcagcca aactaggatt ttcttacacg tttttgtcta cgatagccgt tgagtttttt
    17341 attttcccag tagctattaa agattttttc cttgctttcg cgattgattt ccaaaaagta
    17401 ggcataaatc aaatcgataa agaagagcat aggaagttga gcggatattc gttggatgta
    17461 ggagggttgg ctgtgggtgg ctacaagaac agtctctgta taggtctggc tatttttatt
    17521 gggaacactt gtaaagagta cagtctttgc ccccatctcc ttggcatcta atagactatc
    17581 taaaatagaa ggagttgagc ctgaaagtga gaagcccagt actagacaat tttcatccat
    17641 gatgctggtt gtccaggcaa agccgtcttg gtctgtcaaa gcttcgcaga ccacacctaa
    17701 tcgcataaaa cgtaatttca tttcacgggc gacgaggcca gaactccctg ttccaaagaa
    17761 gtagatacgc tcagcatctt cgattagctg ggcaattcgc tctagttgga tttcgtcaat
    17821 caagtcttgt gtttgttccc tcatattgct ataacttctg aggactcgtt tggtcagtgg
    17881 actgtgcttg gagacttggt tggcttgatt ttctgcctga tgttggtatt ggaaaataaa
    17941 ttctcggtag ccagtaaagc cacacttttt agcaaagcgg gtcaaagcag cttgagaaat
    18001 atgtaatttt tgggtgactt gttgagaaga taaatcatct gtaatcgttt cagcttgcaa
    18061 aaaatagcga gcgatttctt gctctaggtc tgtcatttct tcaaaatgtg aatcaatgat
    18121 agttgcgata tctggtttgt ccatagggaa agctccttta catgagtcat actggaagac
    18181 tagatcaggg aatagtcaca cttcattata acacataata taaggataga taaataaaaa
    18241 cgcatctctg ttttaaaaac gaaaaaatcg aaaaagcttc tctcttttcc ataattttct
    18301 actcaaattg tggtacaatt aagagtaaga ttttaagtta gaaatgagac tgatttgtat
    18361 gagaaaattt aacagccatt cgattccgat tcggcttaat ttattgtttt caatcgtcat
    18421 tttactcttt atgaccatta ttggtcgttt gttgtatatg caggttttga acaaggattt
    18481 ttacgaaaaa aagctagcct cagctagtca gaccaagatt acaagcagtt cagcccgtgg
    18541 ggaaatttat gatgctagtg gaaaaccttt ggtagaaaat acgttaaagc aggttgtttc
    18601 ctttacgcgt agcaataaaa tgacggctac agacttaaaa gaaacagcta aaaagttact
    18661 gacttatgtg agcatcagtt ctccaaattt gacagaacgc cagctggcgg attactattt
    18721 ggctgatcct gaaatctata aaaaaatagt ggaagctctc ccaagtgaga aacgcttgga
    18781 ttcagatggc aatcgtctat ccgaatcaga actgtataac aatgcggtcg atagtgtaca
    18841 aacgagtcaa ctaaactata cagaggatga aaagaaagaa atctatcttt ttagtcagtt
    18901 aaatgctgtt ggaaactttg cgacaggaac cattgcgaca gatcctctaa atgattctca
    18961 ggtggctgtt attgcctcta tttcaaagga gatgcctggc attagtattt ctacttcttg
    19021 ggatagaaag gttttggaaa cttccctttc ttctatagtt gggagtgtat ccagtgaaaa
    19081 agctggtctc ccagcggaag aagcagaagc ctatcttaaa aaaggctatt ctctaaatga
    19141 tcgtgtagga acctcctatt tggaaaagca atatgaagag accttacaag gaaaacgctc
    19201 ggtaaaagaa atccatctgg ataaatatgg caatatggaa agcgtggata caattgagga
    19261 aggtagtaag ggaaacaata tcaaactgac cattgatttg gccttccaag atagcgtgga
    19321 tgctttactg aaaagttatt tcaattccga gctagaaaat ggtggagcca agtattctga
    19381 aggtgtctat gcagtcgccc ttaacccaaa aacaggtgcg gttttgtcta tgtcagggat
    19441 taaacatgac ttgaaaacgg gagagttgac gcctgattcc ttgggaacgg taaccaatgt
    19501 ctttgttcca ggttcggttg tcaaggcggc gaccatcagc tcaggttggg aaaatggagt
    19561 cttgtcagga aaccagacct tgacagacca gtccattgtc ttccaaggtt cagctcccat
    19621 caattcttgg tatactcagg cttacggttc attccctatc acagcggtcc aagctctgga
    19681 gtattcatca aatacctata tggtccaaac agccttaggt cttatggggc aaacctatca
    19741 acccaatatg tttgtcggca ccagcaatct agagtctgct atggagaaac tgcgttcaac
    19801 ctttggcgaa tatggcttgg gtactgcgac aggaattgac ctaccagatg aatctactgg
    19861 atttgttccc aaagagtata gctttgctaa ttacattact aatgcctttg ggcagtttga
    19921 taactatacg ccgatgcagt tggctcagta tgtagcaact attgcaaata atggtgttcg
    19981 tgtggctcct cgtattgttg aaggcattta tggtaataat gataagggag gactgggtga
    20041 cttgattcag caactgcaac cgacagagat gaataaggtc aatatatccg actccgatat
    20101 gagcatcttg caccaaggtt tttatcaggt tgcccatggt actagtgaat tgacaactgg
    20161 acgtgccttt tcaaatggcg ccttggtatc cattagcgga aaaacaggta cagccgaaag
    20221 ctatgtggca gatggtcagc aagcaaccaa taccaatgcg gtggcctatg ccccatctga
    20281 taatccccaa atcgctgttg cagtggtctt tcctcataat accaatctaa caaatggtgt
    20341 aggaccttcc attgcgcgtg acattatcaa tctgtatcaa aaataccatc caatgaacta
    20401 gaaaggaaat tatgctttat ccaacaccta ttgccaagtt gattgacagt tattctaagt
    20461 taccaggtat cgggattaag acggctacgc gtctggcctt ttatacgatt gggatgtctg
    20521 ctgatgatgt caatgaattt gcaaaaaatc tcctttctgc taagagagaa ttgacatatt
    20581 gttctatttg tggacgtttg acagacgacg atccttgttc tatctgtact gatccgactc
    20641 gtgaccagac aacaatttta gttcttgagg atagtagaga tgtggcagcc atggaaaata
    20701 tccaagaata ccatggactc tatcatgtcc ttcatggcct catttctcct atgaatggta
    20761 tcagtccgga cgatatcaat ctcaagagcc ttatgactcg tcttatggat agtgaggttt
    20821 cagaagtgat tgtggcgact aatgctacag cggatggtga agcgacttcc atgtatcttt
    20881 cacgtttgct caagccggct ggtatcaagg ttacgcgtct agcacgaggt ctcgctgtgg
    20941 gagcggacat tgagtatgcg gacgaagtga cactcttacg agcgattgaa aatcggacag
    21001 agttgtaagt gtaggcaaat ttacgaactc cattcattta taaaaaatca aagaggctga
    21061 aaatcgttcc tatcggcctc tttttgtata gtgtgatgag taggatcagg ttcaagtttt
    21121 aaaaaaccaa gcaaatatga tatactaaag agcgagtatt ctagtagaat taggacaaat
    21181 aatatgaaac aaacgattat tcttttatat ggtggacgga gtgcggaacg cgaagtctct
    21241 gtcctttcag ctgagagtgt catgcgtgcg gtcgattacg accgtttcac agtcaagact
    21301 ttctttatca gtcagtcagg tgactttatc aaaacacagg aatttagtca tgctccgggg
    21361 caagaagacc gtctcatgac caatgaaacc attgattggg ataagaaagt tgcaccaagt
    21421 gctatctacg aagaaggtgc agtggtcttt ccagtccttc acgggccaat gggagaagat
    21481 ggctctgttc aaggattctt ggaagttttg aaaatgcctt acgttggttg caacattttg
    21541 tcatcaagtc ttgccatgga taaaatcacg actaagcgtg ttctggaatc tgctggtatt
    21601 gcccaagttc cttatgtggc tatcgttgaa ggcgatgatg tgactgctaa aatcgctgaa
    21661 gtggaagaaa aattggctta tccagtcttc actaagccgt caaacatggg gtctagtgtc
    21721 ggtatttcta agtctgaaaa ccaagaagaa ctccgtcaag ccttagaact tgccttccga
    21781 tatgatagtc gtgtcttggt tgagcaagga gtgaatgccc gtgaaattga ggttggcctc
    21841 ttgggtaact acgatgtcaa gagcacgctg ccaggagaag ttgtcaagga cgttgccttt
    21901 tatgactacg atgccaagta tattgataac aaggttacta tggatattcc tgccaaaatc
    21961 agtgatgatg tggtagctgt catgcgtcaa aatgcagaaa cagccttccg tgccattggt
    22021 ggccttggtc tatctcgttg cgatttcttc tatacagata agggagagat ttttctcaac
    22081 gagctcaata ccatgccagg ttttacccag tggtctatgt acccactact ttgggacaat
    22141 atggggatca gctacccaga actaatcgag cgtttggttg accttgccaa ggaaagtttt
    22201 gacaagcgcg aagcgcattt gatataaaat gaaagagagg gtagaagcca gaaccatcac
    22261 tgcacggtga ctagagttct cggacttcag ccctttttaa aggagtagaa atgaaattaa
    22321 caatccatga aattgcccaa gttgttggag ccaaaaatga tatcagtatc tttgaggaca
    22381 cccagttaga aaaagctgag tttgatagtc gtttgattgg aactggagat ttatttgtgc
    22441 cacttaaagg tgcgcgtgat ggccatgact ttattgaaac agcctttgaa aatggtgcag
    22501 cagtaacctt gtctgagaaa gaggtctcaa atcatcctta cattttagta gatgatgttt
    22561 tgacagcctt tcaatcccta gcatcctact atcttgaaaa aacgactgtt gatgtctttg
    22621 ctgttacagg ttcaaatggc aagacaacga ctaaggatat gttggcgcat ttactgtcaa
    22681 caagatacaa gacctacaaa acacaaggca attacaataa tgagattggc cttccttaca
    22741 cagttctcca catgcctgaa ggaacagaaa agttggtctt ggagatgggg caggaccact
    22801 tgggagatat tcatctcttg tctgaattgg ctcgtccaaa aacagccatc gtgaccttgg
    22861 ttggagaagc tcatttggcc tttttcaaag atcgttctga gattgctaaa ggaaaaatgc
    22921 aaattgcaga tggcatggct tcgaattcct tgctcttggc gccagcagat tcgattgtag
    22981 aggactactt gccaactgat aaaaagatag ttcgatttgg gcaaggagca gagttggaaa
    23041 ttaccgactt ggttgagcgc aaggacagtc tgacctttaa ggctaatttt ttggaacaag
    23101 cccttgattt tccagtgact ggtaagtaca atgccaccaa tgctatgatt gcatcctatg
    23161 ttgccctgca agaaggagtt tcagaggagc aaatccgttt ggccttccaa ggtcttgaat
    23221 tgacgcgtaa ccgtaccgag tggaagaaag cagccaatgg agcagacatc ttgtcagatg
    23281 tttacaatgc caatccaact gctatgaagc tgattttaga gaccttctct gccattccag
    23341 ccaatgaagg tggcaagaaa attgcagtgt tggcggatat gaaggaactt ggtgtccagt
    23401 ctgttcaact tcataatcag atgattttga gcctttcgcc agatgtgctt gatactgtta
    23461 ttttctacgg agaagatatt gctgaattag cccaacttgc tagtcaaatg ttcccaatcg
    23521 gacacgttta ttacttcaag aaaacagaag accaagacca atttgaagac ctagttaagc
    23581 aggtcaaaga aagccttggg gctcatgacc aaatcctgct caaaggctct aactctatga
    23641 atctagccaa gttggtagaa agtttagaaa atgaagacaa gtgattttgt caagtatttg
    23701 caaagaatga ttgccattac agatactgga ttaaccttta caaaagatcc ttttgaccgt
    23761 gagcgctacg aggacttgcg aagtctgtta tctgaaatgt tgaatcaggg atcagacctg
    23821 gatgcagaag aagtagcaga agtcttgaaa ccaacttcag cttatgcgac tcccttaatg
    23881 gacgtccgtg cttggattgt tgaggatgag aagatttgtc tggttagggg acaaggagag
    23941 gatagttggg ctttgccagg tggctttggt gaagtcggct attctccaac tgaaaatatt
    24001 ctcaaggaaa ttgaagaaga aaccggtttt aaagccaaag ttgaaagact gctagctgtt
    24061 tttgatacca atcgtttcca actacagagc aaacaatatg caaagtttgt ttttgaatgt
    24121 aagcttctcg atggacaatt ccaagaaaat caagaaatcg ctgaccttca attttttgca
    24181 attgaccaac tgccgaattt atctgaaaaa cgcattacca aggagcaaat cgagattctt
    24241 tggcaggttt atcaaggtca gagggagcaa tatcttgact aagaagatga ttatcgtatt
    24301 tctaaattca tttttcttaa gtagcatggt ataataaaat gcaggaaaat tttgaatcat
    24361 gaggaagact agatgaattt atgggatatt ttctttacga ctcaggcaac cgagccgccc
    24421 aaatttgacc ttttttggta tgttagccta tttacgctct tagccttaac cttttataca
    24481 gcccatcgct atcgtgaaaa gaaggtttac caacgatttt tccaaatctt gcagactgtt
    24541 cagttaatcc ttctttatgg ttggtactgg gtcaatcata tgccactgtc agaaagccta
    24601 cccttttacc attgccgtat ggctatgttt gtggtactct tgcttcctgg tcagtccaaa
    24661 tataaacaat actttgcatt attgggaaca tttgggacat tagcagcctt tgtttatcca
    24721 gtgccagatg cttacccttt tccacatatc accattctat cctttatctt tggtcattta
    24781 gcactcttgg ggaactctct agtttatcta ttgagacagt ataatgcgcg attgctggat
    24841 gtgaagggaa tttttctcat gacctttgcc ctaaatgcct tgatttttgt ggtcaatttg
    24901 gtgacaggtg gcgattacgg atttttgaca aaaccgccat tggttgggga tcacggtcta
    24961 gtagctaatt atttacttgt ttcaattgtg ctggtagcta ctatcagttt gactaagaaa
    25021 atcttagaat tctttttagc tcaagaagca gaaaaaatga ttgcaaagga agcttaacac
    25081 agagctttct tttttgctct tagagagttt ttacaagcag cttataaaat aagaatttct
    25141 gaatagacaa actcaaaaaa tggctgggaa atttaggaaa aaagcaagca cgattaaatt
    25201 ttttgtgtta taatattttg tgaatagcta tgcctatgtt tagctatgga ataatacgaa
    25261 gtgcgaaact tggaagatag agaggaagcg atgtaatggc tagagaaggc ttttttacag
    25321 gtctagatat tggaacaagc tctgtcaagg tgcttgtggc cgagcagaga aatggtgaat
    25381 taaatgtaat tggcgtgagt aatgccaaaa gtaaaggtgt aaaggatgga attattgttg
    25441 atattgatgc agcagcaact gctatcaagt cagccatttc ccaagcggaa gaaaaggcag
    25501 gcatttcgat taaatcagta aatgtcggct tgcctggtaa tcttttgcag gtagaaccaa
    25561 ctcaggggat gattccagta acatctgata ctaaggaaat tacggatcaa gatgttgaaa
    25621 atgttgtcaa atcagctttg acaaagagta tgacacctga ccgtgaagtc attaccttta
    25681 ttcctgaaga atttattgtg gatggtttcc aagggattcg tgacccacgt ggcatgatgg
    25741 gggttcgcct tgaaatgcgt ggtttgcttt atacaggacc tcgtactatc ttgcacaatt
    25801 tgcgtaagac ggttgagcgt gcaggtgttc aggttgaaaa tgttatcatt tcaccactag
    25861 caatggttca atctgttttg aacgaagggg aacgtgaatt tggtgctaca gtgattgata
    25921 tgggggcagg tcaaacgact gtcgctacaa tccgtaatca agaactccag ttcacacata
    25981 ttctccaaga aggtggagat tatgtaacta aagatatctc caaggttttg aaaacctctc
    26041 gcaaattagc ggaaggcttg aaactgaatt acggggaagc ctatccgcct cttgcaagca
    26101 aagaaacctt ccaagtagag gttattggag aagtagaagc agtcgaagtg acggaagcct
    26161 acttgtcaga aattatttct gcacgaatca agcacatcct tgaacaaatc aagcaagaat
    26221 tagatagaag gcgtctattg gacctccctg gtggtattgt cttaatcggt gggaatgcca
    26281 ttttaccagg tatggttgag cttgctcagg aagtctttgg cgtccgtgtc aagctttatg
    26341 ttctaaatca agttggtatc cgtaatccag cctttgcgca tgtgattagt ttatcagaat
    26401 ttgcgggtca attaacagaa gttaatcttt tggctcaggg agcgataaaa ggtgagaatg
    26461 acttaagtca tcagccaatt agttttggtg ggatgctgca aaaaacagct cagtttgtac
    26521 aatcaacgcc tgttcaacca gctcctgctc cagaagtaga gccggtggcg cctacagaac
    26581 caatggcgga tttccaacaa gcttcacaaa ataaaccgaa attagcagat cgtttccgtg
    26641 gcttgatcgg aagcatgttt gacgaataaa gaggaaaaat aaattatgac attttcattt
    26701 gatacagctg ctgctcaagg ggcagtgatt aaagtaattg gtgtcggtgg aggtggtggc
    26761 aatgccatca accgtatggt cgacgaaggt gttacaggcg tagaatttat cgcagcaaac
    26821 acagatgtac aagcattgag tagtacaaaa gctgagactg ttattcagtt gggacctaaa
    26881 ttgactcgtg gtttgggtgc aggaggtcaa cctgaggttg gtcgtaaagc cgctgaagaa
    26941 agcgaagaaa cactgacgga agctattagt ggtgccgata tggtcttcat cactgctggt
    27001 atgggaggag gctctggaac tggagctgct cctgttattg ctcgtatcgc caaagattta
    27061 ggtgcgctta cagttggtgt tgtaacacgt ccctttggtt ttgaaggaag taagcgtgga
    27121 caatttgctg tagaaggaat caatcaactt cgtgagcatg tagacactct attgattatc
    27181 tcaaacaaca atttgcttga aattgttgat aagaaaacac cgcttttgga ggctcttagc
    27241 gaagcggata acgttcttcg tcaaggtgtt caagggatta ccgatttgat taccaatcca
    27301 ggattgatta accttgactt tgccgatgtg aaaacggtaa tggcaaacaa agggaatgct
    27361 cttatgggta ttggtatcgg tagtggagaa gaacgtgtgg tagaagcggc acgtaaggca
    27421 atctattcac cacttcttga aacaactatt gacggtgctg aggatgttat cgtcaacgtt
    27481 actggtggtc ttgacttaac cttgattgag gcagaagagg cttcacaaat tgtgaaccag
    27541 gcagcaggtc aaggagtgaa catctggctc ggtacttcaa ttgatgaaag tatgcgtgat
    27601 gaaattcgtg taacagttgt cgcaacgggt gttcgtcaag accgcgtaga aaaggttgtg
    27661 gctccacaag ctagatctgc tactaactac cgtgagacag tgaaaccagc tcattcacat
    27721 ggctttgatc gtcattttga tatggcagaa acagctgaat tgccaaaaca aaatccacgt
    27781 cgtttggaac caactcaggc atctgctttt ggtgattggg atcttcgccg tgaatcgatt
    27841 gttcgtacaa cagattcagt cgtttctcca gtcgagcgct ttgaagcccc aatttcacaa
    27901 gatgaagatg aattggatac acctccattt ttcaaaaatc gttaagtaaa tgaatgtaaa
    27961 agaaaataca gaacttgttt ttcgagaagt tgcagaggct agtctgagtg ctaatcgaga
    28021 gtgtggttcg gtctctgtca ttgcagttac caagtatgta gatgtaccga cagcggaagc
    28081 cttgcttccg ctaggtgttc atcatatcgg tgaaaatcgt gtagataagt ttctggaaaa
    28141 atatgaagct ttaaaagatc gagatgtgac ttggcatttg attggtacct tgcaaagacg
    28201 taaggtgaaa gatgtcattc aatacgttga ttatttccat gcattggact cagtaaagct
    28261 agcaggggaa attcaaaaaa gaagtgaccg agtcatcaag tgtttccttc aagtaaatat
    28321 ttctaaagaa gaaagcaaac acggtttttc gagagaggaa ctgctggaaa tcttgccaga
    28381 gttagccaga ctagataaga ttgaatatgt tggtttaatg acgatggcac cttttgaggc
    28441 tagcagtgag cagttgaaag agattttcaa ggcgacccaa gatttacaaa gagaaattca
    28501 agagaaacaa attccaaata tgcctatgac cgagttaagt atgggaatga gtcgtgatta
    28561 taaagaagcg attcaattcg gttccacttt tgttcgtata ggtacatcat tttttaagta
    28621 ggagagaacc atgtctttaa aagatagatt cgatagattt atagattatt ttacggagga
    28681 tgaggattca agtctccctt atgaaaaaag agatgagcct gtgtttactc cagtaaattc
    28741 ttcacaggaa ccggctctcc caatgaatca accttcacag tcggctggta caaaagagaa
    28801 caatatcacc agacttcatg caagacaaca ggaattggca aatcagagtc agcgtgcaac
    28861 ggataaggtc attatagatg ttcgttatcc tagaaaatat gaggatgcaa cagaaattgt
    28921 tgatttattg gcaggaaacg aaagtatctt gattgatttt cagtatatga cagaggtgca
    28981 ggctcgtcgt tgtttggact atttggatgg agcttgtcat gttttagctg gaaatttgaa
    29041 aaaggtagct tctaccatgt atttgttgac accagtgaat gttattgtaa atgttgaaga
    29101 tatccgttta ccagatgaag atcaacaggg tgagttcggt tttgatatga agcgaaatag
    29161 agtacgataa tgattttttt aattcgtatg atttataatg cagtggatat ttactccctg
    29221 attttggtag ccttcgctgt catgtcttgg tttccaggtg cctacgaatc cagtttaggt
    29281 cgttggattg tagcgttggt gaaaccagtg cttgctccct tgcaacgcct gcctttacag
    29341 atagcgggtc ttgatttatc tgtttgggtc gcgattgttt tggttcgatt tttaggagaa
    29401 aacctagtgc gttttctggc gatgatagga tgaataaagg gatttatcag catttctcca
    29461 tagaagatcg tccatttctt gacaagggaa tggaatggat aaagaaggta gaagatagct
    29521 atgctccttt tttaactcct tttatcaatc ctcatcagga gaagctatta aagattttgg
    29581 ccaaaaccta tggtcttgct tgtagcagta gtggggaatt cgtctcgagt gagtatgttc
    29641 gagttttatt atacccagat tatttccaac cagagttttc agattttgaa atatctctcc
    29701 aggaaattgt gtattccaat aaatttgaac atttaacgca tgctaagatt ttagggacag
    29761 tcatcaatca attagggatt gaacggaaac tttttggaga tatcctagta gatgaagaac
    29821 gggcgcagat tatgattaat cagcagtttc ttcttctctt tcaagatgga ctaaagaaaa
    29881 ttggtcgtat acctgtttcg ctggaggaac gtcctttcac cgagaaaata gataagctag
    29941 aacagtatcg agaactggat ttatctgtgt ctagttttcg attagatgtt cttttatcaa
    30001 atgttttgaa actatctagg aatcaagcaa accagttgat tgaaaagaaa cttgtccaag
    30061 taaattatca tgtggtagac aaatcagatt acactgttca agttggagac ttgattagtg
    30121 tgagaaaatt tggtcgcttg agattacttc aagataaggg acaaacgaaa aaagagaaga
    30181 aaaaaataac cgtccagtta ttattaagta agtgaggaat agaatgccaa ttacatcatt
    30241 agaaataaag gacaagactt ttggaactcg attcagaggt tttgatccag aagaagtcga
    30301 tgaattttta gatattgtgg ttcgtgatta cgaagatctt gtgcgtgcga atcatgataa
    30361 aaatttgcgt attaagagtt tagaagagcg tttgtcttac tttgatgaaa taaaagattc
    30421 attgagccag tctgtattga ttgctcagga tacagctgag agagtgaaac aggcggcgca
    30481 tgaacgttca aacaatatca ttcatcaagc agagcaagat gcgcaacgct tgttggaaga
    30541 agctaaatat aaggcaaacg agattcttcg tcaagcaact gataatgcta agaaagtcgc
    30601 tgttgaaaca gaagaattga agaacaagag ccgtgtcttc caccaacgtc tcaaatctac
    30661 aattgagagt cagttggcta ttgttgaatc ttcagattgg gaagatattc tccgtccaac
    30721 agctacttat cttcaaacca gtgatgaagc ctttaaagaa gtggttagcg aagtacttgg
    30781 agaaccgatt ccagctccaa ttgaagaaga accaattgat atgacacgtc agttctctca
    30841 agcagaaatg gaagaattac aagctcgtat tgaggtagcc gataaagaat tgtctgaatt
    30901 tgaagctcag attaaacagg aagtggaaac tccaactcct gtagtgagtc ctcaagttga
    30961 agaagagcct ctgctcatcc agttggccca atgtatgaag aaccagaagt agctccagtg
    31021 catccgacag gtccaacacc agctacagaa actgttgatt cagcaccagg atttgaagca
    31081 ccgcaagaat ccgttacaat tttataagaa acgatgtgag aacaatatct tatccttata
    31141 tttccagcga gcaggagatg gtgtgagtcc tgcattccct aacgataaga ttatcctctc
    31201 aaaaactcaa gtctgaatct agtaagattt gacgtttccc acgttacggg ataagaggga
    31261 gaaagactaa atctttttcc gaataaaggt ggtaccacga ttttcgtcct ttttggaagt
    31321 cgtggttttt aatttgttat tatttataaa ggagatacca tgaaactaaa agataccctt
    31381 aatcttggga aaacagcttt cccaatgcgt gcaggccttc cgaccaaaga gccagtttgg
    31441 caaaaggaat gggaagatgc aaaactttat caacgtcgtc aagaattgaa ccaaggaaaa
    31501 cctcatttca ccttgcatga tggccctcca tacgctaacg gaaatatcca cgttggacat
    31561 gctatgaaca agatttcaaa agatatcatt gttcgttcta agtctatgtc aggattttac
    31621 gcaccattta ttcctggttg ggatactcat ggtctgccaa tcgagcaagt attgtcaaaa
    31681 caaggtgtca aacgtaaaga aatggacttg gttgagtact tgaaactttg ccgtgagtac
    31741 gctctttctc aagtagataa acaacgtgaa gattttaaac gtttgggtgt ttctggtgac
    31801 tgggaaaatc catatgtgac cttgactcct gactatgaag cagctcaaat tcgtgtattt
    31861 ggtgagatgg ctaataaggg ttatatctac cgtggtgcca agccagttta ctggtcatgg
    31921 tcatctgagt cagcccttgc tgaagcagag attgaatacc atgatttggt ttcaacttcc
    31981 ctttactatg ccaacaaggt aaaagatggc aaaggagttc tagatacaga tacttatatc
    32041 gttgtctgga caacgactcc atttaccatc acagcttctc gtggtttgac ggttggtgca
    32101 gatattgatt acgttttggt tcaacctgct ggtgaagctc gtaagtttgt cgttgctgct
    32161 gaattattga ctagcttgtc tgagaaattt ggctgggctg atgttcaagt tttggaaact
    32221 taccgtggcc aagaactcaa ccacatcgta acagaacacc catgggatac agctgtagaa
    32281 gagttggtaa ttcttggtga ccacgttacg actgactctg gtacaggtat tgttcataca
    32341 gcccctggtt ttggtgagga cgattacaat gttggtattg ctaataatct tgaagtcgca
    32401 gtgactgttg atgaacgtgg tatcatgatg aagaatgctg gtcctgaatt tgaaggtcaa
    32461 ttctatgaaa aggtagttcc aactgttatt gaaaaacttg gtaacctcct tcttgcccaa
    32521 gaagaaatct ctcactcata tccatttgac tggcgtacta agaaaccaat catctggcgt
    32581 gcagttccac aatggtttgc ctcagtttct aaattccgtc aagaaatctt ggacgaaatt
    32641 gaaaaagtga aattccactc agaatggggt aaagtccgtc tttacaatat gatccgtgac
    32701 cgtggtgact gggttatctc tcgtcaacgt gcttggggtg ttccacttcc tatcttctac
    32761 gctgaagatg gtacagctat catggtagct gaaactattg aacacgtagc tcaacttttt
    32821 gaagaacatg gttcaagcat ttggtgggaa cgtgatgcca aagacctctt gccagaagga
    32881 tttactcatc caggttcacc aaacggcgag ttcaaaaaag aaactgatat catggacgtt
    32941 tggtttgact caggttcatc atggaatgga gtggtggtaa accgtcctga attgacttac
    33001 ccagccgacc tttacctaga aggttctgac caataccgtg gttggtttaa ctcatcactt
    33061 atcacatctg ttgccaacca tggcgtagca ccttacaaac aaatcttgtc acaaggtttt
    33121 gcccttgatg gtaaaggtga gaagatgtct aaatctcttg gaaatactat tgctccaagc
    33181 gatgttgaaa aacaattcgg tgctgaaatc ttgcgtctct gggtaacaag tgttgactca
    33241 agcaatgacg tgcgtatctc tatggatatc ttgagccaag tttctgaaac ttaccgtaag
    33301 attcgtaaca ctcttcgttt cttgattgcc aatacatctg actttaaccc agctcaagat
    33361 acagtcgctt acgatgagct tcgttcagtt gataagtaca tgacgattcg ctttaaccag
    33421 cttgtcaaga ccattcgtga tgcctatgca gactttgaat tcttgacgat ctacaaggcc
    33481 ttggtgaact ttatcaacgt tgacttgtca gccttctacc ttgattttgc caaagatgtt
    33541 gtttacattg aaggtgccaa atcactggaa cgccgtcaaa tgcagactgt cttctatgac
    33601 attcttgtca aaatcaccaa actcttgaca ccaatccttc ctcacactgc ggaagaaatc
    33661 tggtcatatc ttgagtttga aacagaagac ttcgtccaat tgtcagaatt accagaagtt
    33721 caaacttttg ctaaccaaga agaaatcttg gatacatggg cagccttcat ggactttcgt
    33781 ggacaagcac aaaaagcctt ggaagaagct cgtaatgcaa aagttatcgg taaatcactt
    33841 gaagcacact tgacagttta tccaaatgaa gtggtgaaaa ctctactcga agcagtaaac
    33901 agcaatgtag cacaactttt gatcgtgtct gagttgacca tcgcagaagg accagctccg
    33961 gaagctgccc ttagcttcga agatgtagcc ttcacagttg aacgtgctgc tggtgaagta
    34021 tgtgaccgtt gccgtcgtat tgacccaaca acagcagaac gcagctacca ggcagttatc
    34081 tgtgaccact gtgtaagcat cgtagaagaa aactttgcgg aagcagtcgc agaaggattt
    34141 gaagagaaat aagattgaaa agtctaggca aaattcaatt tgagaagaaa agacaactaa
    34201 ttttatagtc tattaaacgc attgtatcac gtttttgaat acctgatatg atgcgttttt
    34261 tatttatttt aaaaatttgc gaggtatgac tttttatact caacaagaat caaagagaaa
    34321 cttagcaagc taacagtagt aagataaaat aggaatttga
//
LOCUS       DAVLVP010000014        33163 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.014, whole genome shotgun sequence.
ACCESSION   DAVLVP010000014 DAVLVP010000000
VERSION     DAVLVP010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 33163)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33163)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33163
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.014"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            650..2803
                     /locus_tag="VYN69_000638"
     CDS             650..2803
                     /locus_tag="VYN69_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEV1532951.1"
                     /translation="MKFGKRHYRPQVDQMDCGVASLAMVFGYYGSYYFLAHLRELAKT
                     TMDGTTALGLVKVAEEIGFETRAIKADMTLFDLPDLTFPFVAHVLKEGKLLHYYVVTG
                     QDKDSIHIADPDPGVKLTKLPRERFEEEWTGVTLFMAPSPDYKPHKEQKNGLLSFIPI
                     LVKQRGLITNIVLATLLVTVINIVGSYYLQSIIDTYVPDQMRSTLGIISIGLVIVYIL
                     QQILSYAQEYLLLVLGQRLSIDVILSYIKHVFHLPMSFFATRRTGEIVSRFTDANSII
                     DALASTILSIFLDVSTVVIISLVLFSQNTNLFFMTLLALPIYTVIIFAFMKPFEKMNR
                     DTMEANAVLSSSIIEDINGIETIKSLTSESQRYQKIDKEFVDYLKKSFTYSRAESQQK
                     ALKKVAHLLLNVGILWMGAVLVMDGKMSLGQLITYNTLLVYFTNPLENIINLQTKLQT
                     AQVANNRLNEVYLVASEFEEKKTVEDLSLMKGDMTFKQVHYKYGYGRDVLSDINLTVP
                     QGSKVAFVGISGSGKTTLAKMMVNFYDPSQGEISLGGVNLNQIDKKALRQYINYLPQQ
                     PYVFNGTILENLLLGAKEGTTQEDILRAVELAEIREDIERMPLNYQTELTSDGAGISG
                     GQRQRIALARALLTDAPVLILDEATSSLDILTEKRIVDNLMALDKTLIFIAHRLTIAE
                     RTEKVVVLDQGKIVEEGKHADLLAQGGFYAHLVNS"
     gene            2816..4165
                     /locus_tag="VYN69_000639"
     CDS             2816..4165
                     /locus_tag="VYN69_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEV1532952.1"
                     /translation="MKPEFLESAEFYNRRYHNFSSSVIVPMALLLVFLLGFATVAEKE
                     MSLSTRATVEPSRILANIQSTSNNRILVNHLEENKLVKKGDLLVQYQEGAEGVQAESY
                     ASQLDMLKDQKKQLEYLQKSLQEGENHFPEEDKFGYQATFRDYISQAGSLRASTSQQN
                     ETIASQNAAASQTQSEIGNLISQTEAKIRDYQTAKSAIETGASLAGQNLAYSLYQSYK
                     SQGEENPQTKVQAVAQVEAQISQLESSLATYRVQYAGSGTQQAYASGLSSQLESLKSQ
                     HLAKVGQELTLLAQKILEAESGKKVQGNLLDKGKITASEDGVLHLNPETSDSSMVAEG
                     ALLAQLYPSLEREGKAKLTAYLSSKDVARIKVGDSVRYTTTHDAGNQLFLDSTITSID
                     ATATKTEKGNFFKIEAETNLTSEQAEKLRYGVEGRLQMITGKKSYLRYYLDQFLNKE"
     gene            4335..5042
                     /locus_tag="VYN69_000640"
     CDS             4335..5042
                     /locus_tag="VYN69_000640"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEV1532953.1"
                     /translation="MSKQLIYSGKAKDIYTTEDENLIISTYKDQATAFNGVKKERIAG
                     KGVLNNQISSFIFEKLNAAGVATHFVEKLSDTEQLNKKVKIIPLEVVLRNYTAGSFSK
                     RFGVDEGIALETPIVEFYYKNDDLDDPFINDEHVKFLQIADDQQIAYLKEETRRINEL
                     LKVWFAEIGLKLIDFKLEFGFDKDGKIILADEFSPDNCRLWDADGNHMDKDVFRRGLG
                     ELTDVYEIVWEKLQGLK"
     gene            5099..8824
                     /locus_tag="VYN69_000641"
     CDS             5099..8824
                     /locus_tag="VYN69_000641"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HEV1532954.1"
                     /translation="MDKRIFVEKKADFQVKSESLVRELQHNLGLSSLKSIRIVQVYDV
                     FDLTEDLFVPAEKHIFSEQVTDHVLDEVSVQADLANYAFFAIESLPGQFDQRAASSQE
                     ALLLLGSSSDVTVNTAQLYLVNKDIDATELEAVKNYLLNPVDSRFKDITTGIAKQEFS
                     ESDKTIPKLTFFESYTAEDFARYKAEQGMAMEVDDLLFIQDYFKSIGRVPTETELKVL
                     DTYWSDHCRHTTFETELKHIDFSASKFQKQLQSTYDKYIAMREELGRSEKPQTLMDMA
                     TIFGRYERANGRLDDMEVSDEINACSVEIEVDVDGVKEPWLLMFKNETHNHPTEIEPF
                     GGAATCIGGAIRDPLSGRSYVYQAMRISGAGDITAPISETRAGKLPQQVISKTAAHGY
                     SSYGNQIGLATTYVREYFHPGFVAKRMELGAVVGAAPKGNVVREKPEAGDVIILLGGK
                     TGRDGVGGATGSSKVQTVESVETAGAEVQKGNAIEERKIQRLFRNGNVTRLIKKSNDF
                     GAGGVCVAIGELADGLEIDLNKVPLKYQGLNGTEIAISESQERMAVVVRPEDVDAFVA
                     ECNKENIDAVVVATVTEKPNLVMHWNGETIVDLERRFLDTNGVRVVVDAKVVDKDVKL
                     PEERQTSAETLESDTLTVLSDLNHASQKGLQTVFDCSVGRSTVNHPLGGRYQLTPTEA
                     SVQKLPVQHGVTHTASVIAQGFNPYVAEWSPYHGAAYAVIEATARLVAAGANWSKARF
                     SYQEYFERMDKQAERFGQPVAALLGSIEAQIQLGLPSIGGKDSMSGTFEELTVPPTLV
                     AFGVTTADSRKVLSPEFKAVGENIYYIPGQALSAEIDFDLIKKNFAQFEASQADHKVT
                     SASAVKYGGVVESLALATFGNYIGAEVTLPELKTALTAQLGGFVFTSPEEIAGVEKIG
                     QTKADFTLTVNGVKLDGHKLDSAFQGTLEEVYPTEFTQAKELEEVPAVASDVVIKAKE
                     KVEKPVVYIPVFPGTNSEYDSAKAFEKEGAEVNLVPFVTLNEEAIVKSVETMVDNIDK
                     TNILFFAGGFSAADEPDGSAKFIVNILLNEKVRVAIDSFIARGGLIIGICNGFQALVK
                     SGLLPYGNFEAANNTSPTLFYNDANQHVAKMVETRIANTNSPWLAGVQVGDIHAIPVS
                     HGEGKFVVTAEEFAELRDNGQIFSQYVDFNGKPSMDSKYNPNGSVHAIEGITSKNGQI
                     IGKMGHSERYEDGLFQNIPGNKDQHLFASAVKHFTGK"
     gene            8917..10359
                     /locus_tag="VYN69_000642"
     CDS             8917..10359
                     /locus_tag="VYN69_000642"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEV1532955.1"
                     /translation="MTYEVKSLNEECGVFGIWGHPDAAKLTYFGLHSLQHRGQEGAGI
                     LSNDQGQLKRHRDMGLLSEVFRNPANLDKLTGASAIGHVRYATAGEASVDNIQPFLFR
                     FHDMQFGLAHNGNLTNAASLKKELEQRGAIFSATSDSEILAHLIRRSHNPSLMGKIKE
                     ALSLVKGGFAYILLFEDKLIAALDPNGFRPLSIGKMANGAVVVSSETCAFEVIGAEWI
                     RDLKPGEIVIIDDEGIQYDSYTDDTQLAICSMEYIYFARPDSNIHGVNVHTARKRMGA
                     QLAREFKHEADIVVGVPNSSLSAAMGFAEESGLPNEMGLIKNQYTQRTFIQPTQELRE
                     QGVRMKLSAVSGVVKGKRVVMVDDSIVRGTTSRRIVQLLKEAGATEVHVAIGSPALAY
                     PCFYGIDIQTRQELIAANHTVEETRQIIGADSLTYLSIDSLIESIGIETDAPNGGLCV
                     AYFDGDYPTPLYDYEEDYRRSLEEKISFYK"
     gene            10396..11418
                     /locus_tag="VYN69_000643"
     CDS             10396..11418
                     /locus_tag="VYN69_000643"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEV1532956.1"
                     /translation="MTNKNAYAQSGVDVEAGYEVVERIKKHVARTERAGVMGALGGFG
                     GMFDLSKTGVKEPVLISGTDGVGTKLMLAIKYDKHDTIGQDCVAMCVNDIIAAGAEPL
                     YFLDYVATGKNEPAKLEQVVAGVAEGCVQAGAALIGGETAEMPGMYGEDDYDLAGFAV
                     GVAEKSQIIDGSKVVEGDVLLGLASSGIHSNGYSLVRRVFADYTGEEVLPELEGKKLK
                     EVLLEPTRIYVKAVLPLIKEELVNGIAHITGGGFIENVPRMFADDLAAEIDESKVPVL
                     PIFKALEKYGQIKHEEMFEIFNMGVGLMLAVSPENVERVKELLDEAVYEIGRIVKKEN
                     ESVIIK"
     gene            11415..11960
                     /gene="purN"
                     /locus_tag="VYN69_000644"
     CDS             11415..11960
                     /gene="purN"
                     /locus_tag="VYN69_000644"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEV1532957.1"
                     /translation="MKKIAVFASGNGSNFQVIAEEFPVEFVFSDHRDAYVLERAKQLG
                     VLSYAFELKEFESKTDYEAALVELLEEHQIDLVCLAGYMKIVGPTLLSAYEGRIVNIH
                     PAYLPEFPGAHGIEDAWNAGVGQSGVTIHWVDSGVDTGQVIKQVRVPRLADDTIDRFE
                     ARIHEAEYRLYPEVVKALFTD"
     gene            12044..12553
                     /locus_tag="VYN69_000645"
     CDS             12044..12553
                     /locus_tag="VYN69_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000894002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEV1532958.1"
                     /translation="MLESKKTTRYVFYVYLMLLTWGILFKFETNPEFIAFFLAPRYIN
                     WIPFSEPLIVDGKIVFAEMLFNLISFIPLGVCFPLIKTNLSSLRIVGTGFLISLLFEC
                     LQYILAIGITDITDLTLNTLGVCVGLLIYQIFIRVFKSQTRKWINILGMLSLGFAYLV
                     LLLLHLIGV"
     gene            12578..14125
                     /gene="purH"
                     /locus_tag="VYN69_000646"
     CDS             12578..14125
                     /gene="purH"
                     /locus_tag="VYN69_000646"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEV1532959.1"
                     /translation="MTKRVLISVSDKAGIVEFAQELKKLGWEIISTGGTKVALDNAGV
                     DTIAIDDVTGFPEMMDGRVKTLHPNIHGGLLARRDLDSHLEAAKDNKIELIDLVVVNL
                     YPFKETILKPDVTYADAVENIDIGGPSMLRSAAKNHASVTVVVDPADYAVVLDELAAN
                     GETSYETRQRLAAKVFRHTAAYDALIAEYFTAQVGESKPEKLTLTYDLKQPMRYGENP
                     QQDADFYQKALPTDYSIASAKQLNGKELSFNNIRDADAAIRIIRDFKDSPTVVALKHM
                     NPCGIGQADDIETAWDYAYESDPVSIFGGIVVLNREVDAATAEKMHGVFLEIIIAPSY
                     TDEALAILINKKKNLRILALPFNAQEASEVEAEYTGVVGGLLVQNQDVVKESPADWQV
                     VTKRQPTETEATALEFAWKAIKYVKSNGIIVTNDHMTLGVGPGQTNRVASVRLAIDQA
                     KDRLNGAVLASDAFFPFADNVEEIAKAGIKAIIQPGGSVRDQESIEAADKYGLTMVFT
                     GVRHFRH"
     gene            14247..15509
                     /gene="purD"
                     /locus_tag="VYN69_000647"
     CDS             14247..15509
                     /gene="purD"
                     /locus_tag="VYN69_000647"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEV1532960.1"
                     /translation="MKLLVVGSGGREHAIAKKLLESKDVEKVFVAPGNDGMTLDGLEL
                     VNISISEHSKLIDFAKTNDVAWTFIGPDDALAAGIVDDFNQAGLKAFGPTRAAAELEW
                     SKDFAKEIMVKYGVPTATYGTFSDFEEAKAYIEKHGAPIVVKADGLALGKGVVVAETV
                     KQAVEAAHEMLLDNKFGDSGARVVIEEFLEGEEFSLFAFVNGDKFYIMPTAQDHKRAY
                     DGDKGPNTGGMGAYAPVPHLPQSVVDTAVDTIVKPVLEGVIKEGRPYLGVLYAGLILT
                     ADGPKVIEFNARFGDPETQIILPRLTSDFAQNITDILDSKEPNIMWTDKGVTLGVVVA
                     SKGYPLDYERGVELPAKTEGDVITYYAGAKFAENSRALLSNGGRVYMLVTTADTVKEA
                     QASIYQELSQQKIEGLFYRTDIGSKAIK"
     gene            15912..16400
                     /gene="purE"
                     /locus_tag="VYN69_000648"
     CDS             15912..16400
                     /gene="purE"
                     /locus_tag="VYN69_000648"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEV1532961.1"
                     /translation="MKPVISIIMGSKSDWATMQKTAEVLDRFGVAYEKKVVSAHRTPD
                     LMFKHAEEARSCGIKIIIAGAGGAAHLPGMVAAKTTLPVIGVPVKSRALSGVDSLYSI
                     VQMPGGVPVATMAIGEAGATNAALFALRLLSVEDKSIADALANFAEEQGKIAEESSNE
                     LI"
     gene            16387..17478
                     /gene="purK"
                     /locus_tag="VYN69_000649"
     CDS             16387..17478
                     /gene="purK"
                     /locus_tag="VYN69_000649"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEV1532962.1"
                     /translation="MSSSKTIGIIGGGQLGQMMAISAIYMGHKVIALDPAADCPASRV
                     AEIIVAPYNDVDALRQLADRCDVLTYEFENVDADGLDAVIKDGQLPQGTDLLRISQNR
                     IFEKDFLSNKAQVTVAPYKVVTSSLDLADIDLSKNYVLKTATGGYDGHGQKVIRSEAD
                     LEAAYALADSADCVLEEFVNFDLEISVIVSGNGKEVTFFPVQENIHRNNILSKTIVPA
                     RISESLADKAKAMAVRIAEQLNLSGTLCVEMFATDDDVIVNEIAPRPHNSGHYSIEAC
                     DFSQFDTHILGVLGAPLPVIKLHAPAVMLNVLGQHVEAAEQYVTENSSAHLHMYGKIE
                     AKHNRKMGHVTLFSDVPDSVDELGEGIDF"
     gene            17488..17715
                     /locus_tag="VYN69_000650"
     CDS             17488..17715
                     /locus_tag="VYN69_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazole carboxylase"
                     /protein_id="HEV1532963.1"
                     /translation="MIQIIVNTFIEKDKTGAVVEVLYASADQDKVQAKYEELAAQYPE
                     NYLAIYDVPLDTDLNTLDHYPSVFIEKEEFE"
     gene            17778..19076
                     /gene="purB"
                     /locus_tag="VYN69_000651"
     CDS             17778..19076
                     /gene="purB"
                     /locus_tag="VYN69_000651"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEV1532964.1"
                     /translation="MINRYSRPEMANIWSEENKYRAWLEVEILSDEAWAELGEIPKED
                     VALIRKKADFDIDRILEIEQETRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLYKQANDIIRRDLENFTNIIADKAKEHKFTIMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAAGVEAGKISGAVGNFANIPPFVEEYVCNKLGIRAQEISTQVLPRDL
                     HAEYFAVLASIATSIERMATEIRGLQKSEQREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MTGLARVIRGHMITAYENVALWHERDISHSSAERIITPDTTILIDYMLNRFGNIVKNL
                     TVFPENMIRNMNSTFGLIFSQRAMLTLIEKGMTREQAYDLVQPKTAYSWDNQVDFKPL
                     LEADSEVTSRLTQEEIDEIFNPVYYTKRVDDIFERLGLGD"
     gene            complement(19131..23072)
                     /gene="strH"
                     /locus_tag="VYN69_000652"
     CDS             complement(19131..23072)
                     /gene="strH"
                     /locus_tag="VYN69_000652"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000679939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1532965.1"
                     /translation="MKHEKQQRFSIRKYAVGAASVLIGFAFQAQTVAADGVTTTTTTE
                     NQPTIHTVSDSPQSSENRTEETPKAELQPEAPKTVETETPATDKVASLPKTEEKPQEE
                     VSSTPSDKAEVVTPTSAEKETANKKAEEASPKKEEAKEVDSKESNTDKTDKDKPAKKD
                     EAKAEADKPETEAGKERAATVNEKLAKKKIVSIDAGRKYFSPEQLKEIIDKAKHYGYT
                     DLHLLVGNDGLRFMLDDMSITANGKTYASDDVKRAIEKGTNDYYNDPNGNHLTESQMT
                     DLINYAKDKGIGLIPTVNSPGHMDAILNAMKELGIQNPNFSYFGKESTRTVDLDNEQA
                     VAFTKALIDKYAAYFAKKTEIFNIGLDEYANDATNAKGWSVLQADKYYPNEGYPVKGY
                     EKFIAYANDLARIVKSHGLKPMAFNDGIYYNSDTSFGSFDKDIIVSMWTGGWGGYDVA
                     SSKLLAEKGHQILNTNDAWYYVLGRNADGQGWYNLDQGLNGIKNTPITSVPKTEGADI
                     PIIGGMVAAWADTPSARYSPSRLFKLMRHFANANAEYFAADYESAEQALNEVPKDLNR
                     YTAESVAAVKEAEKAIRSLDSNLSRAQQDTIDQAIAKLQETVNNLTLTPEAQKEEEAK
                     REVEKLAKNKVISIDAGRKYFTLDQLKRIVDKASELGYSDVHLLLGNDGLRFLLNDMT
                     ITANGKTYASDDVKKAIIEGTKAYYDDPNGTALTQAEVTELIEYAKSKDIGLIPAINS
                     PGHMDAMLVAMEKLGIKNPQAHFDKVSKTTMDLKNEEAMNFVKALIGKYMDFFAGKTK
                     IFNFGTDEYANDATSAQGWYYLKWYQLYGKFAEYANTLAAMAKERGLQPMAFNDGFYY
                     EDKDDVQFDKDVLISYWSKGWWGYNLASPQYLASKGYKFLNTNGDWYYVIGNHKQDEA
                     YPLSKAVENSGKVPFNQLASTKYPEVDLPTVGSMLSIWADRPSAEYKEEEIFELMTAF
                     ADHNKDYFRANYNALREELAKIPTNLEGYSKESLEALDAAKTALNYNLNRNKQAELDT
                     LVANLKAALQGLKPAATHSGSLDENEVAANVETRPELITRTEEIPFEVIKKENPNLPA
                     GQENIITAGVKGERTHYISVLTENGKTTETVLDSQVTKEVINQVVEVGSPVTHKGDES
                     GLAPTTEVKPRLDIQEEEIPFTTVTRENPLLLKGKTQVITKGVNGHRSNFYSVSTSAD
                     GKEVKTLVNSVVAQEAVTQIVEVGTMVTHVGDENGQAAIAEEKPKLEIPSQPAPSTAP
                     AEESKALPQDPAPVVTEKKLPETGTHDSAELVVAGLMSTLAAYGLTKRKED"
     gene            complement(23392..24108)
                     /locus_tag="VYN69_000653"
     CDS             complement(23392..24108)
                     /locus_tag="VYN69_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1532966.1"
                     /translation="MAIPKYQYIKDELKNKIISGQFSSGDKFYTEAELISMYDVSSIT
                     VVRALNDLAKDGYIVRQQGKGTFVSRARKHKLVEFSDIELFNAKDDKVTVLSIERGNK
                     LVYLEKLGLRGDQFYYKIERIRESNGVVYIYHTSYIPEQYINANYPNLEYYSSIYNRF
                     KLDYHIHMNDEHFEEINEIVFPTPEHAASVLGVDEHFPTVLQTKITKLESTGQVLEYS
                     ETYKRSDYYKIKFISCDRDH"
     gene            24456..26243
                     /locus_tag="VYN69_000654"
     CDS             24456..26243
                     /locus_tag="VYN69_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HEV1532967.1"
                     /translation="MTRFEIRDDFYLDGKSFKILSGAIHYFRVPPEDWYHSLYNLKAL
                     GFNTVETYVAWNLHEPCEGEFHFEGDLDLEKFLQIAQDLGLYAIVRPSPFICAEWEFG
                     GLPAWLLTKNMRIRSSDPVYIEAVGRYYDQLLPRLVPRLLDNDGNILMMQVENEYGSY
                     GEDKAYLRAIRQLMEECGVTCPLFTSDGPWRATLKAGTLIEEDLFVTGNFGSKAPYNF
                     SQMQEFFDEHGKKWPLMCMEFWDGWFNRWKEPIITRDPKELADAVREVLEQGSINLYM
                     FHGGTNFGFMNGCSARGTLDLPQVTSYDYDALLDEEGNPTAKYLAVKKMMATHFSEYP
                     QLEPLYKESMELDAIPLVEKVSLFETLDSLSSPVESLYPQKMEELGQSYGYLLYRTET
                     NWDAEEERLRIIDGRDRAQLYVDGQWVKTQYQTEIGEDIFYQGKKKGLSRLDILIENM
                     GRVNYGHKFLADTQRKGIRTGVCKDLHFLLNWKHYPLPLDNPEKIDFSKGWTQGQPAF
                     YAYDFTVEEPKDTYLDLSEFGKGVAFVNGQNLGRFWNVGPTLSLYIPHSYLKEGANRI
                     IIFETEGQYKEEIHLTRKPTLKHIKGENL"
     gene            26240..26716
                     /locus_tag="VYN69_000655"
     CDS             26240..26716
                     /locus_tag="VYN69_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HEV1532968.1"
                     /translation="MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEK
                     SGLKLATPPGVKLSILPVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETL
                     NVGNMSQTPETRSITRSINVVDKDVEDFHKLAEKGVKLTAQMVPNDPISDFLSLLK"
     gene            26744..27649
                     /locus_tag="VYN69_000656"
     CDS             26744..27649
                     /locus_tag="VYN69_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HEV1532969.1"
                     /translation="MIQWWQILLLTLYSAYQICDELTIVSSAGSPVFAGFITGLIMGD
                     VTTGLLIGGNLQLFVLGVGTFGGASRIDATSGAVLATAFSVSQGIDAPLAITTIAVPV
                     AALLTYFDVLGRMTTTFFAHRVDAAIERFDYKGIERNYLLGAIPWALSRALPVFFALA
                     FGGAFVQSVVDFVEAYKWVADGLTLAGRMLPGLGFAILLRYLPVKRNLHYLAMGFGLT
                     AMLTVLYSYVTGLGGAVAGIVGTLPAEVAEKIGFVNNFKGLSMIGISIVGIFLAVLHF
                     KNSQKVAVAAPSTPSESGEIEDDEF"
     gene            27636..28451
                     /locus_tag="VYN69_000657"
     CDS             27636..28451
                     /locus_tag="VYN69_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000185291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1532970.1"
                     /translation="MTNSNYKLTKEDFNQINKRSLFTFQLGWNYERMQASGYLYMILP
                     QLRKMYGDGTPELKEMMKVHTQFFNTSPFFHTIIAGFDLAMEEKDGVGSKDAVNGIKT
                     GLMGPFAPLGDTIFGSLVPAIMGSVAATMAIAGQPWGIFLWIAVAVAYDIFRWKQLEF
                     AYKEGVNLINNMQSTLTALIDAASVLGVFMMGALVATVINFEISYKLPIGEKMIDFQD
                     ILNQIFPRLLPAIFTAFIFWLLGKKGMNSTKAIGIIIVLALALSALGHFALGM"
     gene            28458..28862
                     /locus_tag="VYN69_000658"
     CDS             28458..28862
                     /locus_tag="VYN69_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007284837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1532971.1"
                     /translation="MTKSLILVSHGRFCEELRGSTEMIMGPQDNIYTVALLPEDGPEE
                     FTAKFEAVIEGLDDFLVFADLLGGTPCNVVSRLIMEGRDIDLYAGMNLPMVIEFINAS
                     LTGADADYKSRAAESIVKVNDLLAGFDDDEDE"
     gene            29161..30327
                     /locus_tag="VYN69_000659"
     CDS             29161..30327
                     /locus_tag="VYN69_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEV1532972.1"
                     /translation="MLHYTKEDLLELGAEITTREIYQQPDVWREAFEFYQAKREEIAA
                     FLQEIADKHDYIKVILTGAGTSAYVGDTLLPYFKEVYDERKWNFNAIATTDIVANPAT
                     YLKKDVATVLVSFARSGNSPESLATVDLAKSLVDELYQVTITCAADGKLALQAHGDDR
                     NLLLLQPAVSNDAGFAMTSSFTSMMLTTLLVFDPTEFAVKSERFEVVSSLARKVLDKA
                     EDVKELVDLDFNRVIYLGAGPFFGLAHEAQLKILELTAGQVATMYESPVGFRHGPKSL
                     INDNTVVLVFGTTTDYTRKYDLDLVREVAGDQIARRVVLLSDQAFGLENVKEVALGCG
                     GVLNDIYRVFPYIVYAQLFALLTSLKVENKPDTPSPTGTVNRVVQGVIIHEYQK"
     gene            30443..31480
                     /locus_tag="VYN69_000660"
     CDS             30443..31480
                     /locus_tag="VYN69_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEV1532973.1"
                     /translation="MKAYTERVFGNVEGEDVLAYRFETDGGYQLEVMTYGATILRYVA
                     PDKAGNFANVILGFDDFDSYVGNSPKHGASVGPVAGRIAGATFELNGKTYDLEVNNAS
                     NCNHSGSTGWDSSLFEVEEVSDHGLTLYTERTDGTGGFPGNLKIWISYHLEETGAYEI
                     SYKVTTDQDTLVNPTNHSYFNLSGDFTQTIDRHVFQLNTEGIYSIAPDGVPAKTPEAN
                     RDVVKHVYNGTLLKDIFAEEDEQIQLASGLDHPFALPAGHDNAGFLYDQNSGRFLLFK
                     TEAPCFVVYTANFVDESVIIGGQPMLQHNGIALEAQALPDAIHSDLKGQVILKAGQTF
                     TSKTRYELVVK"
     gene            31597..32810
                     /locus_tag="VYN69_000661"
                     /pseudo
     CDS             31597..32810
                     /locus_tag="VYN69_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041867.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein C"
BASE COUNT         9162 a         6459 c         7770 g         9772 t
ORIGIN      
        1 ttaattagaa aaaaattttt aaagtcttcg gagaagaaaa cagaaaaatc tgttaaaaaa
       61 taatcaaaac tataaatgat gaatctgaat caaaattatt ttgcgcatgt aaagaggagt
      121 cttatagtaa cgagtcaaaa aaggagtaac tatgaatcgt aatttagaac ggtgttatct
      181 attctgacta ggaatagatc ataccagagg tagcttagaa atagcagaga cattagaaat
      241 tgaagtaata aataggatgt cgtaagtgtt actatcaatg atttatttgt ttcaagcttg
      301 cctagggtga cagtaaaaaa tcaatttcct ttcaatagca tatttttagt gggcaggact
      361 cttgttctgc ctattttttt atccaaaaag tgcagttggg agggagatag gctcatttgg
      421 gaaggaagtc cagtttttgt ttagtgattg gggtaagata gttgttatca gatgagttta
      481 tactcttcga aaatcaaatt caaaccacgt caacgtcgcc ttgccgtata tatgtgactg
      541 acttcgtcag tcctatctac aacctcaaaa cagtgttttg agcagcctgc ggctagtttc
      601 ctagtttgct ctttgatttt cattgagtat tagggaaaag gagatgaata tgaaatttgg
      661 gaaacgtcac tatcgtccgc aagtggatca gatggactgc ggtgtagctt cattagccat
      721 ggtttttggc tactatggta gttattattt tttggctcac ttgcgagaat tggctaagac
      781 gaccatggat gggacgacgg ctttgggctt ggtcaaggtg gcagaggaga ttggttttga
      841 gacgcgagcc attaaggcgg atatgacgct ttttgacttg ccggatttga cttttccttt
      901 tgttgcccat gtgcttaagg aagggaaatt gctccactac tatgtggtga ctgggcagga
      961 taaggatagc attcatattg ccgatccaga tcccggggtg aagttgacta aactgccacg
     1021 tgagcgtttt gaggaagaat ggacaggagt gactcttttt atggcaccta gtccagacta
     1081 taagcctcat aaggaacaaa aaaatggtct gctctctttt atccctatat tagtgaagca
     1141 gcgtggcttg attaccaata tcgttttggc aacactcttg gtaaccgtga ttaacattgt
     1201 gggttcttat tatctgcagt ctatcattga tacctatgtg ccagatcaga tgcgttcgac
     1261 actagggatt atttctattg ggctagtcat cgtctacatc ctccagcaaa tcttgtctta
     1321 cgctcaggag tatctcttgc ttgttttggg gcaacgcttg tcgattgacg tgattttgtc
     1381 ctatatcaag catgtttttc acctccctat gtccttcttt gcgacacgca ggacagggga
     1441 gatcgtgtct cgttttacag atgctaacag tatcatcgat gcgctggctt cgaccatcct
     1501 ttcgattttc ctagatgtgt caacggttgt cattatttcc cttgttctat tttcacaaaa
     1561 taccaatctc tttttcatga ctttattggc gcttcctatc tacacagtga ttatctttgc
     1621 ctttatgaag ccgtttgaaa agatgaatcg ggataccatg gaagccaatg cggttctgtc
     1681 ttcttctatc attgaggaca tcaacggtat tgagactatc aagtccttga ccagtgaaag
     1741 tcagcgttac caaaaaattg acaaggaatt tgtggattat ctgaagaaat cctttaccta
     1801 tagtcgagca gagagtcagc aaaaggctct gaaaaaggtt gcccatctct tgcttaatgt
     1861 cggcattctc tggatggggg ctgttctggt catggatggc aagatgagtt tggggcagtt
     1921 gattacctat aataccttgc tggtttactt taccaatcct ttggaaaata tcatcaatct
     1981 gcaaaccaag cttcagacag cgcaggttgc caataaccgt ctaaatgaag tgtatctagt
     2041 agcttctgag tttgaggaga agaaaacagt tgaggatttg agcttgatga agggagatat
     2101 gaccttcaag caggttcatt acaagtatgg ctatggtcga gacgtcttgt cggatatcaa
     2161 tttaaccgtt ccccaagggt ctaaggtggc ttttgtgggg atttcagggt caggtaagac
     2221 gactttggcc aagatgatgg ttaattttta cgacccaagt caaggggaga ttagtctggg
     2281 tggtgtcaat ctcaatcaga ttgataaaaa agccctgcgc cagtacatca actatctgcc
     2341 tcaacagccc tatgtcttta acggaacgat tttggagaat cttcttttgg gagccaagga
     2401 ggggacgaca caggaagata tcttacgggc ggtcgaattg gcagagattc gagaggatat
     2461 cgagcgcatg ccactgaatt accagacaga attgacttcg gatggggcag ggatttcagg
     2521 tggtcaacgt cagagaatcg ctttggcgcg tgctctcttg acagatgcgc cggtcttgat
     2581 tttggatgag gcgactagca gtttggatat tttgacagag aagcggattg tcgataatct
     2641 catggctttg gacaagacct tgattttcat tgctcaccgc ttgactattg ctgagcggac
     2701 agagaaggta gttgtcttgg atcagggcaa gattgtcgaa gaaggaaagc atgctgattt
     2761 gcttgcacag ggtggctttt acgcccattt ggtcaatagc tagaaagagg agaggatgaa
     2821 accagaattt ttagaaagtg cggagtttta taatcgtcgt taccataatt tttccagtag
     2881 tgtgattgta cccatggccc ttctgctcgt gtttttactt ggctttgcaa ctgttgcaga
     2941 aaaggagatg agtttgtcca ctagagctac tgtcgaacct agtcgtatcc ttgcaaatat
     3001 ccagtcaact agcaacaatc gtattcttgt caatcatttg gaagaaaata agctggttaa
     3061 gaagggggat cttttggttc aataccaaga aggggcagag ggtgtccaag cggagtccta
     3121 tgccagtcag ttggacatgc taaaggatca aaaaaagcaa ttggagtatc tgcaaaagag
     3181 cctgcaagaa ggggagaacc actttccaga ggaggataag tttggctacc aagccacctt
     3241 tcgcgactac atcagtcaag caggcagtct tagggctagt acatcgcaac aaaatgagac
     3301 catcgcgtcc cagaatgcag cagctagcca aacccaatcc gaaatcggca acctcatcag
     3361 tcaaacagag gctaaaattc gcgattacca gacagctaag tcagctattg aaacaggtgc
     3421 ttccttggcc ggtcagaatc tagcctactc tctttaccag tcctacaagt ctcagggcga
     3481 ggaaaatccc caaactaagg ttcaggcagt tgcacaggtt gaagcacaga tttctcagtt
     3541 agaatctagt cttgctactt accgtgtcca gtatgcaggt tcaggtaccc agcaagccta
     3601 tgcgtcaggg ttaagcagtc aattggaatc ccttaaatcc caacacttgg caaaggttgg
     3661 tcaggaattg acccttctag cccagaaaat cttggaggca gagtcaggta agaaggtaca
     3721 gggaaatctt ttagacaagg ggaaaattac ggcgagtgag gatggggtgc ttcatcttaa
     3781 tcctgagacc agtgattcta gcatggttgc agaaggtgcc ctactagctc aactttatcc
     3841 atctttggaa agagaaggga aagccaaact cacagcttat ctaagttcaa aagatgtagc
     3901 aagaatcaag gtcggtgatt ctgttcgcta tactacgact catgatgccg ggaatcaact
     3961 tttcctagat tctactatta caagtattga tgcgacagct actaagactg agaaagggaa
     4021 tttctttaaa atcgaggcgg agactaatct aacttcggag caggctgaaa aacttaggta
     4081 cggggtggaa ggccgcttgc agatgattac gggcaagaaa agttacctac gttattattt
     4141 ggatcaattt ttgaacaaag agtaatgttc gtgtttttag agttaaataa tttttaaact
     4201 gtgagaaaga ttcttcttgc agttttttct ttacaatttt tgaaaaacat ctgctattta
     4261 ttcggttaaa ttcttgtgtt ttttggtttt ttgtggtaaa atgtgctcaa gtaatacgaa
     4321 aggcgaactt taaaatgtca aaacaattga tctattcggg aaaagctaaa gatatctata
     4381 caactgagga tgaaaatctt attatttcaa cttacaagga ccaggcgact gctttcaacg
     4441 gtgtcaagaa ggagcggatt gcaggtaagg gagtcttgaa taatcagatc tcatctttta
     4501 tttttgagaa attaaatgcg gctggtgtgg cgactcactt tgtggagaaa ctttcagaca
     4561 cggaacaact caataaaaag gttaagatta ttcctttgga agtcgtgctc cgcaactata
     4621 ctgctggttc cttttcaaaa cgttttggtg tggatgaggg aatcgccttg gagactccga
     4681 ttgtcgaatt ttactacaaa aatgatgatt tggatgatcc atttatcaat gatgagcatg
     4741 tgaaattcct acagattgcg gatgaccagc agattgccta cttgaaggaa gaaacgcgtc
     4801 gtatcaatga actattgaaa gtctggtttg ctgagattgg gcttaaattg attgacttta
     4861 agctagagtt cggttttgac aaggatggca agattatctt ggcagacgaa ttttcaccag
     4921 ataactgccg cttgtgggac gctgatggca accacatgga taaggatgtt ttccgtagag
     4981 gattgggaga actaaccgac gtttatgaga ttgtttggga aaagttgcag ggtttaaaat
     5041 aacaacctca aggctgtttg ggaatattgc aagagctgaa ataaaggaat aagaattgat
     5101 ggataaacgt atttttgttg aaaaaaaggc tgattttcag gtcaagtcag agagtttggt
     5161 tagagagctc cagcacaact tgggactgtc aagcttgaaa agtattcgta ttgtgcaagt
     5221 atatgatgta tttgacttga ctgaggactt gtttgtacct gcagagaagc acattttctc
     5281 tgagcaggta accgaccatg ttttagatga agtatctgtg caggcggatc ttgctaacta
     5341 tgctttcttt gccattgaaa gtctgccagg gcagtttgac cagcgtgcag cttcgtcaca
     5401 ggaagccttg cttttgttgg gaagttcgag tgacgtgaca gtcaacacag cccaacttta
     5461 cttggtgaat aaagatattg atgcgactga gttggaagct gtcaaaaact acctgctcaa
     5521 tccagttgat tctcgtttca aggatatcac gacagggatt gccaagcagg agttttcaga
     5581 gtcagacaag accattccaa aattgacttt ctttgaaagc tatacagcag aagactttgc
     5641 tcgctacaag gccgagcaag ggatggccat ggaagtggat gatttgctct ttatccaaga
     5701 ctactttaag tcaatcgggc gcgtgccaac tgagactgaa ctcaaggttt tggacactta
     5761 ctggtctgac cactgccgtc atacgacttt tgagacagag ttgaaacaca tcgacttttc
     5821 agcttctaaa tttcaaaagc aattgcagtc aacctatgac aagtatattg ccatgcgcga
     5881 ggagttaggt cggtctgaaa aaccacaaac cttgatggat atggcgacta ttttcggtcg
     5941 ttatgagcgt gctaatggac gattggatga tatggaagtc tctgacgaaa tcaatgcctg
     6001 ctcagttgaa attgaagtgg atgttgatgg tgtcaaggaa ccttggctcc tcatgtttaa
     6061 aaatgaaacc cacaaccatc caacagaaat tgagccattt ggtggagcgg ctacctgtat
     6121 cggtggagct attcgtgatc cgttgtcagg ccgttcctat gtttaccaag ccatgcgtat
     6181 ttcaggtgct ggtgatatta cagcaccgat ttcggaaact cgtgctggga aattgccaca
     6241 acaagtcatt tctaaaacag cagctcatgg ttattcttca tatggtaacc agattgggct
     6301 tgccacaacc tacgttcgtg aatacttcca cccaggcttt gtagctaaac gtatggaact
     6361 tggtgccgtt gttggtgcgg ctcccaaggg caatgttgtc cgtgaaaaac ctgaagcagg
     6421 tgatgtgatc atccttctcg gaggcaaaac aggtcgtgat ggtgtcggtg gtgcgacggg
     6481 ctcttctaag gttcaaacag ttgagtctgt agagactgct ggtgctgagg ttcaaaaagg
     6541 aaatgccatc gaagaacgca agattcagcg cctcttccgt aatggcaatg tcactcgtct
     6601 gatcaagaag tccaatgact ttggggcagg cggcgtctgt gtggctatcg gtgaattggc
     6661 agacggtctt gaaatcgacc tcaacaaggt gcctcttaaa taccagggct tgaatggtac
     6721 agaaattgcc atctctgaat cacaagaacg gatggcggtc gtggttcgtc ctgaagatgt
     6781 ggatgccttc gttgccgaat gtaacaaaga aaatattgat gctgttgtgg tggcgacagt
     6841 aactgaaaaa ccaaatcttg tcatgcactg gaatggtgag acaatcgttg acttggagcg
     6901 tcgtttcctt gacaccaatg gtgtgcgcgt ggttgtcgat gccaaagttg tggacaagga
     6961 tgtcaaactc ccagaagagc gtcaaacatc tgctgaaaca ctggaatcag atacccttac
     7021 ggttctatct gacctcaacc atgcaagtca aaaaggatta cagactgtct ttgactgctc
     7081 tgttggacgc tcaacggtta atcacccact tggtggtcgt taccaactca caccaactga
     7141 ggcatctgtg cagaaattgc cagttcaaca cggtgtgact catactgcgt cggtcattgc
     7201 tcaaggtttc aacccatatg tagctgaatg gtctccatac cacggtgctg cttatgcggt
     7261 tatcgaagca actgctcgtt tggtggctgc tggtgccaac tggtctaagg ctcgtttctc
     7321 ttaccaagaa tatttcgagc ggatggacaa gcaagcagag cgtttcggtc agccagtagc
     7381 tgctcttcta ggttctattg aagcacaaat tcagcttggc ttgccatcta tcggtggtaa
     7441 ggactccatg tctggtacct ttgaagaatt gaccgttccg ccaaccttgg ttgcctttgg
     7501 ggtgacgacg gcagatagcc gtaaggtgct ctctccagaa tttaaagctg ttggggaaaa
     7561 tatctactac atcccaggtc aagccctctc tgcagagatt gattttgact tgattaagaa
     7621 aaattttgct cagtttgaag caagccaagc tgaccataaa gtgacgtctg catcagctgt
     7681 caaatacggt ggtgtagttg aaagtttggc tcttgctacc tttggaaact atattggtgc
     7741 agaggtgacc ttgcctgaac ttaaaacagc tttgacagct caattaggcg gctttgtctt
     7801 cacatctcct gaagaaattg ctggagtaga gaagattgga caaacgaaag cagactttac
     7861 actgactgtc aacggtgtga agctagatgg acacaagctt gacagtgcat ttcaagggac
     7921 attggaagaa gtttacccaa cagaatttac ccaagcgaaa gaactagaag aagtaccagc
     7981 tgtggcatca gatgttgtga ttaaagccaa agaaaaggtt gaaaaacctg tggtttacat
     8041 cccagtcttt ccaggaacca actcagaata tgattcagct aaggccttcg aaaaagaagg
     8101 tgcagaggtc aatttggtac cattcgtgac cttgaatgaa gaagctattg tcaagtcagt
     8161 tgaaactatg gttgacaata tcgacaagac taatattctc ttctttgccg gtggattctc
     8221 ggctgcggat gaaccagatg gttcagctaa gtttatcgtc aatatcctgc ttaatgaaaa
     8281 agtgcgtgtg gctattgata gctttatcgc ccgtggtggc ttgattatcg gtatttgtaa
     8341 tggattccaa gccttagtca aatcgggtct cctaccatac ggaaactttg aagctgctaa
     8401 caatactagc ccaaccctct tctacaatga tgccaaccaa cacgtggcca agatggtgga
     8461 aactcgcatt gccaatacca actcaccatg gttggctggt gtgcaagtgg gcgatatcca
     8521 cgctattcct gtttcgcacg gtgaagggaa gtttgtcgtg acggctgagg aatttgcaga
     8581 gctccgtgac aatggacaaa ttttcagcca atacgttgac tttaacggta aaccaagtat
     8641 ggattctaag tacaatccga atggttctgt ccatgccatc gaaggaatta ccagcaagaa
     8701 tggtcaaatc atcggtaaga tgggccactc agaacgttat gaggatggtc ttttccaaaa
     8761 tatcccaggc aataaagacc aacacctgtt cgcatcagcg gttaaacatt tcactggaaa
     8821 ataagactta cagattttct aatagatagt atcagtaatg taaaagtcat gtaaatctag
     8881 ctcttgatga ttacaaatga aaattaggta taaaaaatga catacgaagt aaaatctctt
     8941 aatgaagaat gtggtgtttt cggtatttgg ggacatccag atgctgctaa gttgacctat
     9001 tttggactcc acagtcttca acaccgtggt caggaggggg caggaatcct ctccaatgat
     9061 caaggacaac tgaagcgcca tcgtgacatg gggcttttat cagaagtttt cagaaatcca
     9121 gccaatttgg ataaattgac aggagctagt gcgattgggc atgtgcgtta tgcgactgct
     9181 ggcgaagctt ctgttgataa catccagccc ttcctcttcc gttttcacga tatgcagttt
     9241 ggcttggctc ataatggaaa tctgaccaat gcagcctctc tcaagaaaga actggaacaa
     9301 agaggagcaa ttttcagcgc gacttcggac tctgaaatct tggctcacct cattcgtcgt
     9361 agtcataatc ctagcctgat gggcaaaatc aaggaagcgc tcagccttgt caaaggtggt
     9421 tttgcctata tcttgctgtt tgaggacaag ttgattgcgg ctcttgaccc taatggcttt
     9481 cgtccgcttt ctatcgggaa aatggccaac ggagcggtgg ttgtttcctc tgaaacctgt
     9541 gcttttgagg tcattggtgc cgagtggatt cgtgatttga agccaggtga gattgtgatc
     9601 attgatgacg agggcattca gtatgacagc tatacagatg atactcagtt ggcgatttgt
     9661 tctatggagt atatctactt tgctcgccct gattctaata tccacggtgt caatgtccat
     9721 acggcacgta agagaatggg agcgcaattg gcgcgagaat ttaagcatga ggcagatatt
     9781 gtagttggtg tgcccaattc ttccctaagc gcggctatgg gatttgcgga agaatcaggc
     9841 ttaccaaatg aaatgggtct gatcaaaaac caatacaccc agcgaacttt tatccaaccg
     9901 actcaagaat tgcgggagca aggagtgcgg atgaaactgt ctgctgtttc gggtgttgtc
     9961 aaaggcaaac gtgtggtcat ggtggatgat tccattgtac gtggaacaac ctctcgtcgt
    10021 atcgttcagc tcttgaaaga agcgggtgcg actgaggttc acgttgccat tggaagtcct
    10081 gcactagcgt atccatgttt ctacgggatt gatatccaga cccgtcagga gctgattgca
    10141 gccaatcata cggtcgaaga aactcgccaa atcattggtg cggacagtct gacttatctt
    10201 tcaattgata gcttgattga gtcgattggt atcgaaacag atgcgccgaa cggtggtctc
    10261 tgtgtcgctt actttgacgg tgactaccca acgcctctct atgactacga agaagactat
    10321 cgtagaagtt tggaagaaaa gatcagtttt tacaagtagg cgacagattc tccattaaag
    10381 aaaaggaaaa aacaaatgac aaataaaaat gcatatgccc agtcgggtgt ggatgttgaa
    10441 gcgggttatg aagttgttga acggattaaa aagcacgtgg cccgtacgga gcgtgcaggt
    10501 gtcatgggag ctcttggtgg ctttggtggt atgtttgacc tttccaagac tggggttaaa
    10561 gaacccgtct tgatttcagg gactgacggt gtcggaacca agctcatgtt ggctatcaag
    10621 tacgacaagc acgataccat cgggcaggac tgtgtggcca tgtgtgtcaa cgacatcatt
    10681 gctgcaggtg cggaacccct ctattttctc gactacgtag cgacagggaa gaatgaacca
    10741 gctaagctag aacaagtggt tgctggtgtg gcagaaggtt gtgtgcaggc tggtgctgcc
    10801 ctcatcggtg gggaaacggc tgaaatgccg ggcatgtacg gcgaagacga ctatgacttg
    10861 gctggttttg cggtcggtgt ggctgaaaaa tctcaaatca ttgacggttc aaaggtggta
    10921 gagggagatg ttcttctcgg acttgcttca agtgggattc actcaaatgg ttactctttg
    10981 gttcgtcgtg tctttgcgga ttacacaggt gaggaagtcc taccagaatt ggaaggcaag
    11041 aaacttaagg aagttctact tgagccgact cgtatctatg tcaaggctgt cttgccgctc
    11101 atcaaagaag agttggtcaa cggcattgcc cacatcacag gtggtggctt tatcgaaaat
    11161 gtccctcgta tgtttgcaga tgacctagct gctgaaattg atgaaagcaa agttccagtg
    11221 cttccaattt tcaaagccct tgaaaaatat ggtcagatta aacacgaaga aatgtttgaa
    11281 atcttcaata tgggtgtggg acttatgttg gcggtcagcc ctgaaaatgt agagcgtgta
    11341 aaagaattgt tggatgaagc agtctatgaa attggtcgca tcgtcaagaa agaaaacgaa
    11401 agtgtcatta tcaaatgaaa aaaatagcgg tttttgcctc tggtaatggc tcaaattttc
    11461 aggtgattgc cgaagaattt ccagtggagt ttgtcttttc agaccatcgt gatgcctatg
    11521 tgcttgagcg tgcaaagcag ctcggcgttc tgtcctatgc ttttgaactc aaggagtttg
    11581 agagcaagac agactacgaa gcagcccttg tcgaactctt ggaagaacac cagattgact
    11641 tggtttgcct agcaggctac atgaaaatcg ttggaccaac cttattgtcg gcttatgaag
    11701 gtcggattgt caacattcat ccagcctact tgccagaatt tccaggagct catgggattg
    11761 aggatgcttg gaatgctggc gtgggtcagt ctggtgtgac cattcactgg gtggattcgg
    11821 gtgtggatac aggccaggtc atcaaacagg ttcgtgtgcc acgactagct gatgatacca
    11881 ttgacagatt tgaagctcgc atccatgaag cagagtacag gttgtatccg gaagtagtga
    11941 aggctctatt tacagattga ctttttgatg attcatatga tatctttgat tttaaattgg
    12001 agtcagtgtt tgttgaagac ggcttcaaac ggaggtattt gtaatgttag aatctaaaaa
    12061 aacaactcga tatgtatttt atgtctatct gatgttatta acttggggaa tcttatttaa
    12121 gtttgaaaca aatcctgaat ttatagcatt tttcttagct ccaaggtata tcaattggat
    12181 tccattttca gaaccactaa tagtcgatgg aaaaattgtt tttgctgaaa tgttatttaa
    12241 tctgatttcc tttattccat taggtgtttg tttccctttg ataaaaacta atttatctag
    12301 tttaagaata gtcgggacag gtttcttgat tagtttattg tttgagtgct tacagtatat
    12361 tttagcaata ggtataacag atataacgga tttgacttta aatacgctag gtgtctgtgt
    12421 aggcttactg atttatcaaa tttttataag agtgttcaaa tcacagacta gaaaatggat
    12481 caatatctta ggtatgctta gccttggttt tgcttatctt gttttactgt tactgcattt
    12541 aattggtgtt taactaatga ttaaaaagga gaatataatg actaaacgcg tcttaatcag
    12601 cgtctcagac aaagcgggca ttgttgaatt tgcccaagaa ctcaaaaaac ttggttggga
    12661 gattatctca acaggtggaa ctaaggttgc ccttgataat gctggggtgg ataccattgc
    12721 gatcgatgat gtgactggtt tcccagaaat gatggacggt cgtgtgaaga ccctccaccc
    12781 aaatatccac ggagggcttc tcgctcgtcg tgacttggat agccacttgg aagcggctaa
    12841 ggacaacaag attgagctca ttgaccttgt ggtggtcaac ctttacccat ttaaggaaac
    12901 tatccttaaa ccagatgtga cttatgctga tgcagttgaa aatatcgata ttggtgggcc
    12961 gtctatgctt cgttcagcag cgaaaaatca tgccagtgtt acagttgtgg tagatcctgc
    13021 tgactacgct gtggttttgg acgaattggc agcaaacggc gaaacctctt atgaaactcg
    13081 ccaacgttta gcagccaaag tatttcgtca cacagcggct tatgacgcct tgattgcaga
    13141 atacttcaca gctcaagtgg gtgaaagcaa gcctgaaaaa ctcactttga cttatgacct
    13201 caagcaacca atgcgttacg gtgagaatcc tcaacaagac gcggactttt accagaaagc
    13261 tttgcctaca gattactcta ttgcttcagc caaacagctc aacgggaaag aattgtcatt
    13321 taataatatc cgtgatgcag atgctgctat ccgtatcatc cgtgacttca aagatagtcc
    13381 aaccgttgtg gctctcaaac acatgaatcc atgtggaatt ggtcaagctg atgacatcga
    13441 gactgcttgg gactacgctt atgagtctga cccagtatct atctttggtg ggattgtcgt
    13501 cctcaaccgt gaggtggatg ctgcgacagc tgagaagatg cacggcgttt tcctcgaaat
    13561 catcattgca ccaagctata cggatgaagc gctagctatt ttgatcaata aaaagaaaaa
    13621 cttgcgtatc cttgccttgc catttaatgc tcaagaggct agcgaagtgg aagcagaata
    13681 cacaggtgta gtcggtggac ttctcgtgca aaatcaagac gtggtcaagg aaagcccagc
    13741 tgactggcaa gtggtgacca aacgtcagcc aactgagaca gaagcgactg ctcttgagtt
    13801 cgcttggaag gctatcaagt acgtcaaatc aaatggtatt atcgtgacca acgaccacat
    13861 gacacttggt gttggtccag gtcaaaccaa ccgtgtggct tctgttcgcc ttgccattga
    13921 ccaagccaaa gatcgtctga acggggcggt ccttgcttca gatgccttct tcccatttgc
    13981 ggataacgtg gaagaaatcg ccaaagcagg aattaaggcc atcatccagc ccggtggctc
    14041 tgtccgtgac caagaatcca tcgaagcagc ggataaatac ggcttgacta tggtctttac
    14101 aggtgtgaga cattttagac attaagaaga taaaagggaa gaaaacagtt tctttccttt
    14161 tttggcttaa aatgctaact gaaacaagat taaaacgaac ttttttgata taatgttgtt
    14221 aaataattcg caaaagaggt tgaggaatga aactgcttgt tgtcggttct ggtggtcgtg
    14281 agcatgcgat tgctaaaaag ttacttgaat caaaagacgt ggaaaaagtc tttgtagctc
    14341 ctgggaatga tgggatgact ctggatggtt tggaattggt aaatatctct atttccgaac
    14401 attctaaatt gattgacttc gcaaagacca atgatgttgc ttggaccttt atcggtccag
    14461 atgatgccct tgctgctggt atcgtggatg attttaacca agctggactt aaggcctttg
    14521 gtccgactag ggctgcagcg gagctggagt ggtccaagga tttcgccaag gaaatcatgg
    14581 tcaaatacgg cgttccgaca gcaacatatg gcacattttc agatttcgag gaagccaagg
    14641 cctatatcga aaagcatggt gcgcctatcg tagtcaaggc ggatggcttg gcacttggga
    14701 agggtgtcgt cgttgctgag acggttaagc aagcggtcga agccgctcat gagatgcttt
    14761 tggacaataa atttggtgac tcaggtgcgc gcgtggttat tgaggaattc cttgaaggag
    14821 aggaattttc actctttgcc tttgtcaatg gtgataagtt ctacatcatg ccaacggctc
    14881 aggaccacaa acgtgcctat gatggcgaca aagggcctaa cacgggtggt atgggtgcct
    14941 atgcgccagt cccacactta ccacagagtg tagttgatac agcggttgac accattgtca
    15001 agccagttct agaaggggtg attaaagaag gtcgccctta tctgggagtt ctttacgcag
    15061 ggcttatcct gacagctgat ggaccgaaag tcattgagtt caacgctcgg ttcggagatc
    15121 cagaaactca gattatcttg cctcgcttga cctctgactt tgctcaaaat atcacagata
    15181 tcctggatag caaggagcca aatatcatgt ggacggacaa gggtgtgact ctgggtgtgg
    15241 ttgtcgcatc caagggctac ccgctagact atgaaagggg cgttgagttg ccagcaaaga
    15301 cagaaggcga tgtcatcacc tactatgcag gggctaagtt tgcggaaaat agcagagcac
    15361 tgctctcaaa cggcggacga gtttatatgc tcgttaccac agcagatacc gtcaaagaag
    15421 cccaagccag catctaccaa gaactatccc aacaaaaaat agaaggactc ttctaccgaa
    15481 cagatatcgg aagcaaggca attaagtaaa gatataagaa taacgcgccg tagtcgccaa
    15541 acacgataat ggtcgtcgtg gtgaaaagac cagaacagtg aatgttctgg tcagggggaa
    15601 acttggagac cttaggctca aagtttagga atgaaaccga aggtttgctt ccgcctccat
    15661 cacctaagac cattatcaaa aagaaaaata aaaattcaca aaatacgtta atgatcgtat
    15721 ggtttgcgag cgttagcgag ctaatataga acaatcaccg ccgttgtgaa agaacgattg
    15781 gatgataatc caatcgttca gggaaattgg aagaccttgg gtttccaatt taggcatgag
    15841 acacctttgg tggctgctgc cgtccctcac aagctaaggt gattgttgaa aaagaggaaa
    15901 aaggagaaga aatgaaacca gtaatttcca tcatcatggg ctcaaaatcc gactgggcaa
    15961 ccatgcaaaa aacagcagaa gtcctagacc gcttcggtgt agcctacgaa aagaaagttg
    16021 tttccgcaca ccgtacacca gacctcatgt tcaaacatgc agaagaagcc cgtagttgtg
    16081 gcatcaagat catcatcgca ggtgctggtg gcgcagcgca tttgccaggc atggtagctg
    16141 ccaaaacaac ccttccagtc attggtgtgc cagtcaagtc tcgtgctctt agtggagtgg
    16201 attcactcta ttctatcgtt cagatgccgg gtggggtgcc tgttgcgacc atggctatcg
    16261 gtgaagctgg agcgactaac gcagctctct ttgccctccg tctcctctct gtagaagata
    16321 agtccattgc ggatgcactt gccaactttg ctgaagaaca aggaaaaatc gcagaggagt
    16381 cgtcaaatga gctcatctaa aacaatcgga attatcggtg gcggtcaact gggtcagatg
    16441 atggccattt ctgctatcta catgggccac aaggttatcg cgctggatcc tgcggcggat
    16501 tgcccggcct ctcgcgtggc ggaaatcatt gtggcacctt ataacgatgt ggacgccctc
    16561 cgtcagttgg cagaccgttg cgatgtcctc acttacgagt ttgaaaatgt cgacgctgac
    16621 ggtttggatg ccgttatcaa ggatggacaa ctccctcaag gaacagatct gctccgcatt
    16681 tcgcaaaatc gtatttttga aaaggacttt ttgtcaaaca aggctcaagt cactgtggca
    16741 ccctacaagg tcgtgacttc tagcctagac ttggcagata tcgacttgtc gaaaaactat
    16801 gtcctcaaga ctgcgactgg tggctacgat ggtcatggac aaaaggttat tcgttcagaa
    16861 gcagacttgg aagcagccta tgcgctagca gactcagcag actgcgtctt ggaagaattt
    16921 gtcaactttg accttgagat ttctgtcatc gtgtcaggaa atggcaagga ggtgacgttt
    16981 ttcccagttc aggaaaatat ccaccgcaac aatatcctgt ctaagaccat cgtaccagcc
    17041 cgcatttctg aaagtctagc agacaaggct aaagctatgg cagtgcgaat cgcagaacaa
    17101 ctcaacttgt ctggaactct ctgtgtggaa atgtttgcga ctgatgatga cgttattgtc
    17161 aatgaaatcg ccccacgacc acataactct gggcactatt ctattgaagc ctgtgatttc
    17221 tctcagtttg acacccatat tctcggtgtt ctcggagcac cattaccagt catcaaactc
    17281 catgcgccag ccgttatgct taatgtcctc ggtcagcatg tcgaggctgc tgagcaatat
    17341 gtgacagaaa attcaagcgc ccacctccac atgtatggta aaatagaagc gaagcacaac
    17401 cgcaagatgg gacatgtgac cttgtttagt gatgtaccgg atagtgtgga tgagcttggg
    17461 gaagggattg atttttagga caagtctatg atacaaatta tcgttaatac atttattgaa
    17521 aaggataaga ctggagcagt tgttgaagtg ttgtatgcca gtgctgacca agataaggta
    17581 caagctaaat atgaagaact agctgcacaa taccccgaaa attatttagc tatctatgat
    17641 gtaccgctgg atacggattt gaatacacta gatcattacc cgtctgtgtt tattgaaaaa
    17701 gaggagtttg agtagaaatc ttggtttacc tagatagctt attcccaaca gcttaagaag
    17761 aaaggaaaaa ttaacacatg atcaaccgtt actctcgccc tgagatggcg aatatttgga
    17821 gtgaagaaaa taaataccgt gcttggcttg aggtggaaat cctctctgac gaggcatggg
    17881 ctgagttggg ggaaatccct aaggaagatg tggctttgat tcgcaagaag gcggactttg
    17941 acatcgaccg tattttggaa attgagcagg agacgcgcca cgatgtggtg gctttcacgc
    18001 gtgcggtttc tgagactctt ggtgaagagc gcaagtgggt tcactatggg ttaacttcta
    18061 ccgacgtggt ggatactgct tatggttacc tctacaagca ggccaacgac atcatccgtc
    18121 gtgaccttga aaacttcact aatatcatcg ctgacaaggc caaggagcac aagttcacca
    18181 tcatgatggg gcgtactcat ggtgtgcacg ctgagccgac aacttttggt cttaaattgg
    18241 caacttggta tagcgaaatg aaacgcaata tcgagcgttt cgagcatgcg gctgctggtg
    18301 tagaagctgg taagatttct ggtgcggttg ggaactttgc caatatccca ccatttgtag
    18361 aggagtatgt ctgcaacaag cttggtatcc gtgctcaaga aatctctaca caagtccttc
    18421 ctcgtgacct tcacgctgag tactttgcag ttcttgccag cattgcgact tcaatcgaac
    18481 gtatggcgac tgagattcgt ggtctacaaa aatctgagca acgcgaagta gaagagttct
    18541 ttgctaaagg gcaaaaaggg tcttcagcaa tgcctcacaa acgcaaccca atcggttctg
    18601 aaaatatgac tggtctggcg cgtgtcattc gtggtcacat gattacggct tatgaaaacg
    18661 tcgctctctg gcacgaacgc gatatttccc actcatcagc tgagcgtatc atcacaccag
    18721 atacgaccat tttgattgac tacatgctca accgttttgg aaatatcgtc aagaacttga
    18781 cagtcttccc agaaaatatg atccgaaaca tgaactcgac ttttggtctt atctttagcc
    18841 aacgggctat gttgacattg attgaaaaag gcatgacccg tgagcaagcc tatgacttgg
    18901 tgcaaccaaa aacagcctac tcttgggaca accaagtaga ctttaaacca cttcttgagg
    18961 cagattcaga agtaacatca cgtctcactc aagaagaaat cgatgaaatc ttcaacccag
    19021 tttattacac caaacgagtg gatgatatct ttgaacgtct tggactaggt gattaattaa
    19081 aaaataaaca gcgagcttca atctcgctgt ttatttttta tcgaaaagac ttagtcttct
    19141 tttcttttag tgagtccata ggctgctagt gtggacatga gtcctgcgac tactagttct
    19201 gcagaatcgt gagttcctgt ttcaggaagt tttttctctg ttaccacagg agctggatct
    19261 tgaggaagag ctttgctttc ctcagcagga gcagttgatg gagctggttg gcttgggatt
    19321 tctagttttg gtttttcttc agcaatagcg gcttgtccgt tttcatcgcc tacatgtgtt
    19381 accatagttc cgacttcgac tatttgagta acggcttcct gtgctacgac actatttaca
    19441 agtgttttca cttccttacc atcggcagaa gtgctcacag agtagaagtt gctacgatgt
    19501 ccattgacgc ccttagtaat gacttgtgtt tttcctttga gtaagagtgg attttcacga
    19561 gtcactgtgg taaatggaat ttcttcttct tggatatcca gtctaggttt tacctcagta
    19621 gttggtgcaa gaccactttc atcacccttg tgagttacag gagagccaac ttcaaccact
    19681 tggtttataa cttctttggt tacctggcta tcaaggactg tttctgttgt ttttccattt
    19741 tcagtgagta cagagatgta atgagttcgt tcacctttga ctcctgctgt gataatattt
    19801 tcctgaccag ctgggaggtt aggattttct ttcttgataa cttcaaatgg aatttcttca
    19861 gttcttgtga tgagttctgg tctggtttca acattggcag ccacttcatt ttcatctagg
    19921 cttcctgaat gagttgcagc tggtttgagg ccttgaagag cggcttttag gttggctaca
    19981 agtgtgtcaa gctcagcttg tttattacgg ttgaggttgt aatttagagc tgttttagct
    20041 gcgtcaaggg cctcaagact ttctttacta tatccttcta agtttgtagg aattttagct
    20101 aattcttcgc ggagagcatt ataattagca cggaagtagt ctttgttgtg gtctgcaaag
    20161 gcagtcatga gttcaaagat ttcctcttcc ttgtactcag cgcttggtct atctgcccag
    20221 attgaaagca tacttccgac tgttggaaga tctacttcag gatatttggt agaagctagt
    20281 tgattgaatg gaactttacc agaattttca actgcttttg aaagagggta agcttcatct
    20341 tgtttgtgat tgcctataac ataataccag tcaccgttgg tattcaagaa tttatagcct
    20401 ttgcttgcta ggtattgagg tgatgcgagg ttatatcccc accagccttt agaccagtaa
    20461 gaaatcaaga catctttgtc aaactgaaca tcgtccttgt cttcatagta gaagccatcg
    20521 ttgaaggcca ttggttgaag ccctctttct ttggccatag ctgcgagggt gttggcatat
    20581 tcggcaaatt tgccatagag ttgataccac ttgaggtagt accagccttg ggcactagtc
    20641 gcatcgttgg cgtattcgtc agtaccaaag ttgaaaatct ttgttttacc tgcaaagaag
    20701 tccatgtatt taccgatgag ggcttttaca aagttcatcg cttcttcgtt tttcaagtcc
    20761 atagttgttt ttgaaacttt atcaaagtgg gcttgaggat ttttaatacc taatttttcc
    20821 atggcaacca gcatagcatc catgtgacct ggactgttaa tagctgggat gagaccaatg
    20881 tccttagatt tagcgtattc aattagctct gtcacttctg cctgtgttag tgcagtaccg
    20941 tttggatcgt cgtagtaagc tttagttcct tcgataatag cttttttaac gtcatcacta
    21001 gcataggttt ttccgttggc agtaatggtc atatcattga gtagaaagcg aagtccgtca
    21061 tttcctagaa ggagatggac atcagaatat ccgagctcac tggccttgtc tacgatgcgt
    21121 ttgagctggt ccagagtaaa gtatttgcgt ccagcatcga ttgagattac cttgtttttg
    21181 gcaagttttt caacctcacg tttagcttct tcttcttttt gagcttcagg cgtgagggtc
    21241 aagttgttga cagtttcttg aagtttagca atggcttgat caatcgtatc ttgttgggca
    21301 cggctaaggt tgctatcgag agagcgaata gctttttcag cttcttttac ggccgcgacg
    21361 ctttctgcag tataacggtt caggtctttt ggtacctcgt taagtgcttg ctctgcagat
    21421 tcataatcag ctgcgaagta ttcagcgttg gcatttgcaa aatgacgcat gagtttgaag
    21481 aggcgtgatg gtgaataacg tgcagatgga gtgtcagccc aagcagctac cataccaccg
    21541 atgattggga tatcagctcc ttctgttttt ggtacagaag tgattggtgt gtttttaata
    21601 ccatttagcc cctgatcgag attgtaccag ccttggccat cagcgtttcg tccaagaacg
    21661 tagtaccaag catcattggt attaaggatt tggtgacctt tttcagctag tagtttagaa
    21721 gaagcgacat cgtagcctcc ccaaccacca gtccacatag aaacgatgat gtctttgtca
    21781 aaactaccaa agcttgtgtc gctattgtag tagataccgt cgttaaaggc cattggtttg
    21841 agaccgtgcg attttacaat acgagcgagg tcattggcgt aggcaataaa tttttcatag
    21901 ccttttacag ggtagccttc gtttggatag tatttatcag cttgaagcac actccaacct
    21961 ttagcatttg tcgcatcatt ggcatattca tcaagtccga tgttgaagat ttcagtcttt
    22021 ttcgcgaaat aagcagcata cttgtcgata agggcttttg taaaagcgac agcttgttcg
    22081 ttgtcaagat cgacagtacg ggttgattcc ttcccgaaat agctaaagtt agggttttgg
    22141 attcccaatt ctttcatggc attgagaatc gcatccatgt gaccaggact atttactgtc
    22201 ggaatgagac cgataccttt atctttggca tagttaatca gatctgtcat ttgactttct
    22261 gttaagtgat tgccgtttgg atcgttgtaa taatcatttg tacctttttc aatggcgcgt
    22321 ttgacatcgt cactggcata ggtcttgccg ttagctgtga tgctcatatc gtccaacatg
    22381 aaacggagtc catcatttcc gactaatagg tgtaaatcag tgtagccata atgtttcgct
    22441 ttatcgatga tttccttgag ctgttctggt gagaaatatt tacgtccagc atcaatagaa
    22501 acaattttct ttttcgctag tttttcattt acagttgcag cacgttcctt tcctgcctct
    22561 gtttccggtt tgtcagcctc tgctttcgct tcatcttttt tagctggttt atccttgtca
    22621 gtcttgtctg tatttgactc tttagaatca acctctttcg cttcttcctt tttagggcta
    22681 gcttcttctg cctttttatt agcagtttct ttttcagcag aagttggagt taccacctct
    22741 gctttatcac taggagttga actaacttcc tcttgtggtt tttcttctgt ttttggaaga
    22801 ctagctacct tatcagtagc tggagtttct gtttctacag tttttggagc ttctggttga
    22861 agctctgctt taggtgtttc ctcagtccga ttttcggatg attgagggga atcagaaact
    22921 gtatggatgg tcggttggtt ttctgtagta gtagtagtag taactccatc ggctgcaaca
    22981 gtctgtgctt ggaaggcaaa tccaattaga acagaagctg ctcctacagc gtatttacga
    23041 atagaaaaac gctgttgttt ttcatgtttc attgcaaaac ctcctgattg cattgttata
    23101 ttgatagcga ttatataaat caacgccctt attttatttc ttatattaac gagagtcaag
    23161 aggagatgac aaaaaactat aataagtata aaaaaatata aaatttaaac ttaagatttc
    23221 agattggtcg gaaaaaatgc atatatatat atatctaata taatttttgg ttctatttct
    23281 ataaaatatt ccacaaatta tagaatcttc caaaaatagg taagcgctac ctttttggtg
    23341 tggtataata agcatagaaa aagcccgagc gattaggctc aggctttctt cttagtgatc
    23401 acggtcacat gagatgaatt taatcttgta gtaatcagat cgtttgtaag tttcactgta
    23461 ttctaaaact tggccagttg attcgagttt ggtgatttta gtttgtagga cagtagggaa
    23521 atgttcatcg actccgagga ctgaagccgc atgttctgga gttggaaaga ctatttcgtt
    23581 gatttcttca aagtgttcat cattcatgtg aatgtggtag tctaacttga aacgattata
    23641 gatagaacta tagtattcaa ggtttggata atttgcgttg atatattgtt ctgggatgta
    23701 ggatgtatgg tagatataaa cgacaccgtt tgattcgcgg atacgttcaa tcttgtagta
    23761 gaattgatcg ccgcgtagac ccaatttttc caagtaaaca agcttgtttc cgcgttcaat
    23821 tgaaagaaca gttaccttat catctttagc attgaagagt tcaatatctg aaaactctac
    23881 aagcttgtgt ttgcgtgcac gtgaaacgaa ggttcctttt ccttgttggc ggacaatata
    23941 gccatctttg gcaaggtcgt ttaaggcgcg aacaactgtg atagagctga catcgtacat
    24001 tgaaatgagt tctgcttcag tgtaaaattt atctccactg ctaaactgcc cagagatgat
    24061 tttatttttt aattcgtctt ttatgtattg atatttggga attgccatat ttttacctct
    24121 attttccttc tccatagaaa attttaccat aaaagcgaaa aaaatagtag tcttttgaat
    24181 aaaaaattag aataatagtt tgaaaaatta agcgatatag ggattgctca tattttatca
    24241 atgtattcta tacaaaatta ttttaggtcc tatattccga atttaaagag cgtttgaaat
    24301 gggtctaaaa atgtttgaaa ctaattgaat gtgtgtataa tagtaaaaat gcagtaaata
    24361 tcccgacaat ttctattgac aattcaaaca gattggttta taattaatat aacaacaaat
    24421 gaaagcgcaa actttcgcgg tcggaaggta gttttatgac acgatttgag atacgagatg
    24481 atttctatct cgatggaaaa tcatttaaga ttttatctgg tgccattcat tattttaggg
    24541 ttcctccaga ggattggtat cattcgctct ataacttgaa ggctcttggt tttaatacgg
    24601 tagagactta tgttgcttgg aatttacacg agccttgtga aggtgagttt cattttgaag
    24661 gtgatctgga tttagagaaa tttctccaaa tagcgcagga tttgggtctc tacgcaattg
    24721 tgcgtccgtc tccatttatc tgtgcggaat gggaattcgg tggcttacca gcttggctct
    24781 tgaccaagaa catgcgaatt cgctcatccg acccagtata tatcgaggca gttggtcgct
    24841 actatgatca gttattgcca agactggtgc ctcgtttgtt ggacaatgat ggcaatattc
    24901 tcatgatgca ggttgaaaat gagtatggtt cttacggaga agataaggct tacctgagag
    24961 cgattcgaca gctaatggaa gagtgtggcg taacctgtcc cctctttaca tcggatggtc
    25021 catggcgagc tactctgaaa gctggaacct taattgaaga ggacctcttt gtaacaggaa
    25081 actttggttc taaggcacct tacaactttt cgcagatgca ggaattcttt gatgaacatg
    25141 gtaagaaatg gccactcatg tgtatggagt tctgggatgg ttggttcaat cgctggaaag
    25201 aaccgattat cacacgggat cctaaggaat tggcagatgc agttcgagaa gttttggaac
    25261 aaggctccat caatctttac atgttccacg gtggtacaaa ctttggtttc atgaatggtt
    25321 gctcggctcg aggaactttg gacctgccac aagttacgtc ttatgattac gatgcccttc
    25381 tggatgaaga aggaaatcca actgctaaat atcttgcagt caagaagatg atggcaacac
    25441 atttttcaga gtatccgcag ttggaaccac tctacaaaga gagtatggag ttggatgcta
    25501 ttccactagt tgaaaaagtt tctttgtttg aaaccttaga tagcttgtca agtcctgtag
    25561 aaagtctcta tcctcaaaag atggaggagc tgggacaaag ttatggctac ctactttatc
    25621 gaacagaaac aaactgggat gcagaagaag aaagacttcg tatcattgat ggtcgagata
    25681 gggcccagct gtatgtcgat ggtcagtggg ttaaaactca atatcagaca gagattgggg
    25741 aagatatttt ttatcaaggt aaaaagaaag ggctatctag gttagatatc ttgatagaaa
    25801 atatggggcg tgtcaactat gggcataagt tcttagcgga tacgcaacgt aagggaattc
    25861 ggacaggggt ctgtaaggat ctgcatttct tactaaactg gaaacactat ccactcccac
    25921 tagacaatcc tgagaaaatt gatttttcaa aaggatggac tcaaggacaa ccagcctttt
    25981 acgcttatga ctttacagtc gaagagccaa aagatactta cctagacttg tctgagtttg
    26041 gtaagggggt tgcctttgtc aatgggcaga atctaggacg tttttggaac gttggcccaa
    26101 ctctctcact ttatatccct catagctatc tcaaggaagg tgccaaccgc atcattatct
    26161 ttgaaacaga aggtcaatat aaagaagaga ttcatttaac tcgtaaacct acactaaaac
    26221 atataaaggg ggaaaactta tgacaattgt aggatgccgt attgatggac gtttgatcca
    26281 cggacaagta gccaatcttt gggctggaaa actaaatgtt tcacgcatta tggttgtaga
    26341 cgacgaagtt gtcaacaacg atattgaaaa gagtggtttg aaacttgcga caccaccagg
    26401 tgtgaaattg agtattttgc cagttgagaa agctgcagcc aatattcttg ctggcaaata
    26461 cgatagccaa cgtctcttta tcgtggctcg taaaccagac cgcttccttg gtttggtaga
    26521 agcaggtgta ccacttgaaa cccttaatgt tgggaatatg tctcaaacac cagaaactcg
    26581 ttctattaca cgttctatca acgtagtaga caaggatgtg gaagacttcc acaaactggc
    26641 agaaaaaggt gttaaactta ctgctcagat ggttccaaat gatccaattt cagacttttt
    26701 gagcttatta aaataggaaa aaaattttta ggaggtcatt gttatgatac aatggtggca
    26761 aattttactt ctcactttgt actcagctta tcaaatctgt gatgagttga cgatcgtttc
    26821 atctgcaggt tcccctgtat ttgctggttt cattactggt ttaatcatgg gagatgtgac
    26881 tactggttta cttatcggtg gtaacttgca actgttcgtt cttggggttg gtaccttcgg
    26941 tggtgcttct cgtatcgacg caacttctgg tgcggttctt gcgacagcct tctctgtttc
    27001 acaaggaatt gatgcaccgc ttgccattac tacaatcgct gtaccagtag cagctctctt
    27061 gacttacttc gacgttcttg gtcgtatgac tactaccttc ttcgctcacc gtgtggatgc
    27121 tgcaatcgaa cgctttgact ataaaggtat tgaacgcaac tacttgcttg gtgcgattcc
    27181 gtgggctcta tctcgtgccc ttccagtctt ctttgccctt gcttttggtg gtgcctttgt
    27241 acaatcagta gtagacttcg ttgaagccta caaatgggtt gcagatggct tgacacttgc
    27301 aggacgtatg cttccaggtc ttggatttgc aatcttgctt cgttaccttc cagttaaacg
    27361 taaccttcac taccttgcta tgggatttgg tttgacagct atgttgactg ttctttactc
    27421 atatgtaaca ggtcttggtg gcgctgttgc tggtatcgta ggtactcttc ctgctgaagt
    27481 tgctgaaaaa attggtttcg tgaacaactt caaaggtttg tctatgattg gtatttctat
    27541 cgtaggtatt ttccttgcag tgcttcactt caaaaatagc caaaaagtag ctgtagcagc
    27601 accttctaca ccatcagaaa gtggggaaat cgaagatgac gaattctaat tacaaactta
    27661 caaaagaaga ttttaatcaa atcaacaaac gtagcttgtt tactttccaa ttaggttgga
    27721 actacgaacg tatgcaagct tctggttacc tttacatgat cttgcctcag ttgcgtaaaa
    27781 tgtatggtga tggaactcct gaattgaaag aaatgatgaa agttcatact caattcttca
    27841 atacttcacc attcttccat accattatcg ctggttttga ccttgccatg gaagaaaaag
    27901 atggtgtagg ttcaaaagac gccgttaacg gtatcaagac aggtttgatg ggaccattcg
    27961 ctcctcttgg ggatacaatc tttggttcac ttgtacctgc tatcatgggg tcagtcgcag
    28021 caactatggc tatcgctggc caaccttggg ggatcttcct ttggattgca gttgcagtag
    28081 cgtatgacat cttccgttgg aaacagttgg aatttgctta caaagaaggg gttaacctta
    28141 tcaacaacat gcaaagtacc ttgacagctt tgattgacgc tgcatctgta cttggtgtct
    28201 tcatgatggg tgctcttgta gcaacagtga ttaactttga aatttcttac aagttgccaa
    28261 tcggtgaaaa gatgattgat ttccaagaca tcttgaacca aatcttccca cgtttgcttc
    28321 cagcaatctt tactgccttt atcttctggt tgcttggtaa gaaaggtatg aactctacta
    28381 aagctatcgg tattattatc gtacttgctt tggctctttc tgcccttggt cactttgcac
    28441 ttggaatgta attccttatg actaaatcat taattttggt gagccatggt cgcttctgtg
    28501 aggagcttag aggtagcaca gaaatgatta tgggcccaca agacaacatt tacacagtag
    28561 ctcttcttcc agaagatggc ccagaagaat ttactgctaa atttgaagct gttattgaag
    28621 gattggatga tttcctagtc tttgcggatc ttctcggtgg gacaccttgt aatgtggtga
    28681 gtcgcttgat catggaaggt cgtgatattg acctttacgc agggatgaat cttccaatgg
    28741 tgattgaatt tatcaatgcg agccttacag gcgcagatgc ggactacaag agccgtgctg
    28801 cagaaagcat tgtgaaagtt aatgacctgt tagcgggctt cgatgatgac gaagatgaat
    28861 aatactcttc gaaaatctct tcaaactacg tcaacgtcgc cttgccgtag gtatatgtta
    28921 ctgacttcgt cagtcttatc cggcaacctc aaaacggtgt tttgagctga cttcgtcagt
    28981 cttatccggc aacctcaaag cagtgctttg agcagcctgc ggctagtttc ctacagattt
    29041 tagttggaac tcgattcaat tcatgtgaca acgtgaaaat cgttagagca ttttatatag
    29101 aatatacatg ggaatgtagc ttactcccat tcccatattt aatagaaaaa gaggaactca
    29161 atgctacatt atacaaaaga agacttgctc gaattgggtg cagaaatcac tacgcgtgaa
    29221 atctaccaac agcctgatgt atggagagaa gcttttgaat tttatcaagc aaaacgtgaa
    29281 gaaattgcag ccttcctaca agaaatcgct gataaacatg actatattaa ggttatcttg
    29341 acaggtgctg ggacttctgc ttatgtggga gataccttgc taccttattt taaggaagtc
    29401 tatgacgaac gcaaatggaa tttcaatgct attgcgacaa cagatatcgt tgccaatcca
    29461 gcaacctatt tgaaaaaaga tgtggcaact gtccttgtgt cttttgctcg tagtgggaat
    29521 tcgcctgaaa gtttggcgac tgttgatttg gccaaatcct tggtggatga gctttatcaa
    29581 gtgacgatta cttgtgcagc agatggtaaa ttggctcttc aagctcacgg tgatgatcgt
    29641 aatctcttgc tcttgcaacc agctgtctct aatgatgctg gatttgccat gacttctagc
    29701 tttacgtcta tgatgttgac aactctcttg gtctttgatc ctacagaatt tgctgttaag
    29761 tctgaacgtt ttgaagttgt atctagtctt gcccgtaaag ttttagacaa ggcagaagat
    29821 gtcaaagagc tcgttgattt agactttaac cgtgtcatct atctaggcgc tggtcctttc
    29881 tttggacttg ctcatgaagc tcagctcaag attttggaat taactgctgg tcaagttgcg
    29941 accatgtatg aaagcccagt tggcttccgt cacggtccaa aatctcttat caacgacaat
    30001 acagttgttt tggtctttgg tacaacgaca gactacactc gtaagtacga cttggacttg
    30061 gttcgtgaag ttgctggtga ccagattgct cgtcgtgttg tgcttttgag tgatcaagct
    30121 tttggtcttg aaaatgtcaa agaagtggcc cttggttgtg gcggtgtctt gaatgatatt
    30181 taccgtgtct tcccttacat cgtttatgcc caactctttg ctttattgac ttcactcaag
    30241 gtagaaaata aaccagatac accgtctcct acaggtacag taaaccgtgt agtacaaggt
    30301 gtcataattc acgaatatca aaagtaagac agtgtttatg aattcttgac aagaggattt
    30361 gtaaattatc agataaacca tagattgtca gtacgctttc tatggtttgt ttgcttgaga
    30421 gaaatagtaa aaggagaaca gaatgaaagc atacacagag cgtgtatttg gaaatgttga
    30481 gggtgaggat gtcttggcct atcgatttga gacagacggt ggctaccaac ttgaggttat
    30541 gacttatggt gcgactatct tgcgctatgt cgcacctgac aaggctggaa attttgccaa
    30601 tgttatcttg ggatttgatg actttgatag ttatgtaggc aatagtccca agcatggagc
    30661 aagtgtaggt cctgtagcgg gtcgtattgc aggtgcgacc tttgagctca atggtaagac
    30721 ctatgacctt gaggttaata atgctagcaa ctgtaatcac agtggttcaa ctggttggga
    30781 ttccagcttg tttgaagttg aagaagtaag cgatcatggc ttgactctct acacagagcg
    30841 tacagatggg acaggagggt tccctggaaa tctcaagatt tggatcagtt atcacttgga
    30901 agaaactggt gcctatgaaa tcagctacaa ggtaacgacc gatcaggata cgctggtcaa
    30961 tccaaccaac cacagctatt tcaacttgtc tggtgatttc acgcagacga ttgaccgtca
    31021 tgtcttccaa ctaaacacag agggcattta ctcaatcgct cctgacggtg ttcctgccaa
    31081 aactccagaa gccaaccgtg atgtggtcaa acacgtctac aatggtacct tgttgaagga
    31141 tatctttgca gaagaagatg agcaaatcca gctggcatca ggtttggatc atccatttgc
    31201 ccttcctgca ggccatgaca atgctggatt cctttatgac caaaattcag gtcgcttcct
    31261 gcttttcaag acagaagctc cttgctttgt ggtctacaca gcaaactttg tggatgaaag
    31321 tgtcatcata ggaggtcagc caatgctaca gcacaatggg attgctcttg aagcgcaagc
    31381 tttaccagat gccattcaca gtgaccttaa aggccaagtc attcttaaag ctggtcaaac
    31441 cttcaccagt aagacacgtt atgaacttgt tgtgaagtaa aagagtcatt gcgcctactt
    31501 ttgggagcta ggaataggta cgcagagaca aatagtagga aaatatgata taactaagcg
    31561 ttgaaagcta tctgttaata taatattcaa actacaataa ggagtaagaa agaaacgaag
    31621 aaaattgtat ttgctagtgc cttggctttg accttggctg gagcagtttt gacaaatgat
    31681 gtttttgcga acgacagact tgtggcaaca caaactactg atggtaaaaa tgaaaatgta
    31741 ttgacctcag aggtgctaaa accttctagt ggcaatgttt tggttggaat caaaggagaa
    31801 tttgtggctc ctcatcaaca atctattttg gatgccatca atgctatctg taaagaagcg
    31861 gctgacgaag gtttggtaga taagtatgtc cctatcaaat ggtcaactga cctagaaaag
    31921 gcagcttttg ccagagctac agaagcatct ataaccatgg atcatacccg tctttctagc
    31981 aaagatcttt ggagtgcctt tccaacttct aatagtataa tgggagaaaa tttggcatgg
    32041 aatcatgacg gttttctaaa agctattgaa caatggcgtg ctgaaaaagc agattatgtg
    32101 gagaaaaaaa tagtggttca gacaacggga aatctggtca ctatgagtcg ctaattaacc
    32161 ctaaatttac acacatgggg atggcagctt ttaaaaatcc taacaatcaa tacaaagcta
    32221 ttacaattgc tcaaactcta ggtgatgatg cttcttcaga ggaattggct ggtagatatg
    32281 gttctgctgt tcagtgtaca gaagtgactg cctcaaacct ttcaacagtt aaaactaaag
    32341 ctacggttgt agaaaaacca ctgaaagatt ttagagcgtc tacgtctgat cagtctggtt
    32401 gggtggaatc taatggtaaa tggtatttct atgagtctgg tgatgtgaag acaggttggg
    32461 tgaaaacaga tggtaaatgg tactatttga atgacttagg tgtcatgcag actggatttg
    32521 taaaattttc tggtagctgg tattacttga gcaattcagg tgctatgttt acaggctggg
    32581 gaacagatgg tagcagatgg ttctactttg acggctcagg agctatgaag acaggctggt
    32641 acaaggaaaa tggcacttgg tattaccttg acgaagcagg tatcatgaag acaggttggt
    32701 ttaaagtcgg accacactgg tactatgcct acggttcagg agctttggct gtgagcacaa
    32761 caacaccaga tggttaccgt gtaaatggta atggtgaatg ggtaaactag gctcaggcca
    32821 taggtaaagc attcatctta cttagcaaaa agaatgaacg ataagaaaga ggttgatggc
    32881 gaacattggc ctcttttgat ttataaagat tggattcttg tcgcctcaat ttcagacttt
    32941 tctattgtaa gctaatattt tatagcccat taaaagcata agcggtaatc taatttaaaa
    33001 aatgctgtaa ttagtctgaa gtccacactt acttgttgag atgttatctc tgttttttat
    33061 cgttataatt tactgtattt tttatagtat gcagaatatt tttaagtata tttcaataga
    33121 aatttctatc gatttattgt ataatgataa gtaattgttg aaa
//
LOCUS       DAVLVP010000015        32873 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.015, whole genome shotgun sequence.
ACCESSION   DAVLVP010000015 DAVLVP010000000
VERSION     DAVLVP010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 32873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32873
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.015"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(162..644)
                     /gene="greA"
                     /locus_tag="VYN69_000662"
     CDS             complement(162..644)
                     /gene="greA"
                     /locus_tag="VYN69_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018370745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEV1532974.1"
                     /translation="MAEKTYPMTLEEKEKLEKELEELKLVRRPEVVERIKIARSYGDL
                     SENSEYEAAKDEQAFVEGQISSLETKIRYAEIVNSDAVAQDEVAIGKTVTIQEIGEDE
                     EEVYIIVGSAGADAFAGKVSNESPIGQALIGKKTGDTATIETPVGSYDVKILKVEKTA
                     "
     gene            complement(715..2370)
                     /gene="mltG"
                     /locus_tag="VYN69_000663"
     CDS             complement(715..2370)
                     /gene="mltG"
                     /locus_tag="VYN69_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEV1532975.1"
                     /translation="MSEKSREEEKLSFKEQILRDLEKVKGYDEVLKEDEAVVRTPANE
                     PSAEELMADSLSTVEEIMRKAPTVPTHPSQGVPASPADEIQRETHGVPSHPSQDVPSS
                     PAEESGSRPGPGPVRPKKLEREYNETPTRVAVSYTTAEKKAEQAGPETPTPATETVDI
                     IRDTSRRSRREGAKPVKPKKEKKSHVKAFVISFLVFLALLSAGGYFGYQYVLDSLLPI
                     DANSKKYVTVGIPEGSNVQEIGTTLEKAGLVKHGLIFSFYAKYKNYTDLKAGYYNLQK
                     SMSTEDLLKELQKGGTDEPQEPVLATLTIPEGYTLDQIAQTVGQLQGDFKESLTAEAF
                     LAKVQDETFISQAVAKYPTLLESLPVKDSGARYRLEGYLFPATYSIKESTTIESLIDE
                     MLAAMDKNLSPYYSTIKSKNLTVNELLTIASLVEKEGAKTEDRKLIAGVFYNRLNRDM
                     PLQSNIAILYAQGKLGQNISLAEDVAIDTNIDSPYNVYKNVGLMPGPVDSPSLDAIES
                     SINQTKSDNLYFVADVTEGKVYYANNQEDHDRNVAEHVNSKLN"
     gene            complement(2451..2945)
                     /locus_tag="VYN69_000664"
     CDS             complement(2451..2945)
                     /locus_tag="VYN69_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1532976.1"
                     /translation="MKIRQARLEDLDRIVELEFENFSVEEAIPPSVFEAHLREIQTSF
                     LVAEKEGRIMGYIEGPVGLHRHLQDQSFTEEIKDYSHEPGGYISVTCLSIAKEAQGLG
                     LGQKLLTALKEVALEDERDGINLTCHDYLIAYYEKHGFVNEGQSQSTFAGETWYDMVW
                     EMKK"
     gene            complement(2942..3406)
                     /locus_tag="VYN69_000665"
     CDS             complement(2942..3406)
                     /locus_tag="VYN69_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1532977.1"
                     /translation="MEIPIKIIQASKFDLPEIGALQTSSFPAEKQQLSHILEESIRKC
                     ADTFLLARDENQLLGYILSSPQSDNPQCLKVHSLVIESDHQRQGLGTLLLAALKEVAV
                     ELDYKGIRLESPDELLSYFEMNGFVDEEEILLYATSQGYSMIWFNPFYLEEQ"
     gene            complement(3416..4750)
                     /locus_tag="VYN69_000666"
     CDS             complement(3416..4750)
                     /locus_tag="VYN69_000666"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEV1532978.1"
                     /translation="MSKTYHFIGIKGSGMSALALMLHQMGHKVQGSDVEKYYFTQRGL
                     EQAGITILPFDEKNLDGDMEIIAGNAFRPDNNVEIAYADQNGISYKRYHEFLGSFMRD
                     FVSMGVAGAHGKTSTTGMLSHVLSHITDTSFLIGDGTGRGSANAKYFVFESDEYERHF
                     MPYHPEYSIITNIDFDHPDYFTSLEDVFNAFNDYAKQITKGLFVYGEDAELRKITSDA
                     PIYYYGFEAEGNDFVASDLLRSTTGSTFTVHFRGQNLGQFHIPTFGRHNIMNATAVIG
                     LLYTAGFDLNLVREHLKTFAGVKRRFTEKIVNDTVIIDDFAHHPTEIIATLDAARQKY
                     PSKEIVAVFQPHTFTRTIALLDDFAHALNQADAVYLAQIYGSAREVDHGDVKVEDLAN
                     KINKKHQVITVENVSPLLDHDNAVYVFMGAGDIQTYEYSFERLLSNLTSNVQ"
     gene            complement(4761..5378)
                     /locus_tag="VYN69_000667"
     CDS             complement(4761..5378)
                     /locus_tag="VYN69_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1532979.1"
                     /translation="MKQERFPLVSDDEVMLTEMPVMNLYDESDLISNIKGEYRDKNYL
                     EWAPITEEKPVKPIEKQVEKPKKAPLGVKKEGKSYAEVAREEARADLKKKRSANYLTQ
                     DFSLARRHSQPSLVRQGNQPTTPFQKENPGEFVKYSQKLTQSHYILAEEVHSIPTKNE
                     EVSAPAPKKNNYDFLKKSQIYNKKSKQTEQERRVAQELNLTRMTE"
     gene            complement(5426..8524)
                     /locus_tag="VYN69_000668"
     CDS             complement(5426..8524)
                     /locus_tag="VYN69_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1532980.1"
                     /translation="MAKLIPGKIRIEGVALYETGKVDIIKEKNNRLYARVAEEELRYS
                     LEDDLVFCACDFFQKRGYCVHLAALEHFLKNDERGQEILWSLEEGHEEKEAVETKVTL
                     GGKFLDRILSPKSECAYELSAVGQVEAGTNHILWTLRIGQINSQKYYVIRDIPLFLRI
                     VEQRKSYMIGKIYEESLSWEAFDDASQELLTFLHGLTEEGLVPDLFFQNQGRHLFFPL
                     TFFEQGVNLLMALPHFQFDHQVDSYQTLLFQDMHADANLFAFTVTEYSDYFEMEISES
                     PRVNVFYQGAVLFHKGQVYFLTDQQMRLLKEIKVLPVGQQGKKYLQFDSSDRDKLASC
                     LTLFIQMGTVSAPERLQIKTFAPSFYFDREEDNRIRLEIQFDYGNRQVSSRQELEELP
                     FSSDADLEERIFQVCLAAGFEADFQSWRQALKAESVYHFFHDIIPVFEKLGHVDLSDK
                     LEELYSLASPQVQIASKGGLLEIQFDFQDIAQEEIDQAMQALVANQDFYIDSSNQVYF
                     FDEETKKIRQNLQELGQFELKDGTLQARKSLAYSLAHLFEGRDRVSFSQEFQNLAQDL
                     THPEDFPLQATQVKADLRDYQEKGIGWLQMLHHYGFGGILADDMGLGKTLQTIAFLTS
                     QVTKESRVLILAPSGLIYNWADEFQKFAPQLDVAVVHGLKASREEILAESHQIYVTSY
                     ATFRQDSEFYQGMAFDFLFLDEAQVMKNAQTKIAQTLRQFVVPSVFALSGTPIENHLG
                     ELWSIFQIVMPGLLPSKKEFMKLPAERVAQFIKPFVMRRKKEEVLTELPDLIEVVYKN
                     ELEDQQKAIYLAQLQQMRDHLAQVSEQEFQRSRVEILSGLMRLRQICDTPALFMEDYQ
                     GASGKLDSLRDLLVQVADGGHRVLIFSQFKGMLEKIEQELPDLGLTSFKITGSTPAKE
                     RQDMTKAFNQGERDAFLISLKAGGVGLNLTGADTVILVDLWWNPAVEAQAIGRAHRMG
                     QEETVEVYRLVTKGTIEEKIQELQEQKKHLVSQVLDGTESRGSLTLAEIREILGISEA
                     ST"
     gene            complement(8617..9783)
                     /locus_tag="VYN69_000669"
     CDS             complement(8617..9783)
                     /locus_tag="VYN69_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000521357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1532981.1"
                     /translation="MGKYDFTSLPNRLGHHTYKWKETETDSEVLPAWIADMDFVVLPE
                     IRQAVQTYADQLVYGYTYASEDLIKEVQKWEATQYGYNFDKEALVFIEGVVPAISTAI
                     QTFTKEGEAVLINTPVYPPFARSVKLNNRRLITNSLVEKDSLFEIDFDQLEKDLVEEE
                     VKLYVLCNPHNPGGRVWEKEVLEKIGQLCQKHGVLLVSDEIHQDLTLFGHKHQSFNTI
                     NPAFKNFAIVLSSATKTFNIAGTKNSYAVIENPKLRLAFQKRLLANNQHEISGLGYLA
                     TEAAYRYGKDWLEELKQVFEDHINYVMDLFGKETKIKVMKPQGTYLIWLDFSAYDLTD
                     ETLQELLRNEAKVILNRGLDFGEEGSLHARINIAMPKSLLQEVCQRIVATFAKR"
     gene            complement(9791..10885)
                     /locus_tag="VYN69_000670"
     CDS             complement(9791..10885)
                     /locus_tag="VYN69_000670"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEV1532982.1"
                     /translation="MSKELHINTILAQAGIKSDEATGALVTPLHFSTTYQHPEFGRST
                     GFDYTRTKNPTRSKAEEVLAAIESADYALATSSGMSAIVLAFSGFPVGSKVLAVRDLY
                     GGSFRWFNQVEQEGRFHFTYANTEEELIAELEKDVDVLYIETPTNPLMLEFDIEKLAK
                     LAHAKGAKVVVDNTFYSPIYQRPIEDGADIVLHSATKYLAGHNDVLAGVVVTNSLELY
                     EKLFYNLNTTGAVLSPFDSYQLLRGLKTLSLRMERSTANAQEVVAFLKDSPAVKEVLY
                     TGRGGMISFKVADETRIPHILNSLKVFSFAESLGGVESLITYPTTQTHADIPAEVRHS
                     YGLTDDLLRLSIGIEDARDLIADLRQAIEG"
     gene            complement(11043..12725)
                     /locus_tag="VYN69_000671"
     CDS             complement(11043..12725)
                     /locus_tag="VYN69_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1532983.1"
                     /translation="MKLKLLRVDTKVIMGSFLLVLSSLLALLLPLILKGLIDGSSIEN
                     IGSKVFQSFLIFIGQALFSSIGYYLFSQSGEKKIAKIRKKVIEGLIYAEKSFFDKSQS
                     GELTSAIVNDTSVIREFLITTFPNIILSLVMVLGSIVVLFSLDWNLSLLLFITLPCMM
                     FIILPLSNISEKYSRRLQEEIGFLTGQLTEKIQEHELIKTNQAEKSVQDVLDNCIERV
                     QNNSLKSDRVTSFETPFALLFIFATIVVMLTYGGYRVSAGYISVGTLVSFLIYLFQLL
                     NPISNIANFVTIYSRSKGSSVALENLLAVPKEKFEGGKSVSGQGLNFNHVYFGYDENR
                     PVLKDITCSIFKGQKIAFVGPSGSGKSTIVRLLERFYKPLSGYILMEQSSIYDFNLKE
                     WRSKIAWVSQNNAVLSGSIRDNLCLGLNRLVTDDELMKVLDLVSLGDEIRSMKEGLDT
                     EVGERGRFLSGGQSQRLQIARTYLKDAEILIFDEATANLDADSEYAIISILYSALKEK
                     TVVIIAHRLSTVKDVDCIFFLEGGKITGSGTHKELLENHERYARFVQEQMIE"
     gene            complement(12897..14855)
                     /locus_tag="VYN69_000672"
     CDS             complement(12897..14855)
                     /locus_tag="VYN69_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000748873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1532984.1"
                     /translation="MKKSKSKYLTLAGLVLGTGVLLSACGNSSTASKTYNYVYSSDPS
                     SLNYLAENRAATSDIVANLVDGLLENDQYGNIIPSLAEDWTVSQDGLTYTYKLRKDAK
                     WFTSEGEEYAPVTAQDFVTGLQYAADKKSEALYLVQDSVAGLDDYITGKTSDFSTVGV
                     KALDDQTVQYTLVKPELYWNSKTLATILFPVNADFLKSKGDDFGKADPSSILYNGPFL
                     MKALVSKSAIEYKKNPNYWDAKNVFVDDVKLTYYDGSDQESLERNFTAGAYTTARLFP
                     NSSSYEGIKEKYKNNIIYSMQNSTSYFFNFNLDRKSYNYTSKTSDIEKKSTQEAVLNK
                     NFRQAINFAFDRTSYGAQSEGKEGATKILRNLVVPPNFVSIKGKDFGEVVASKMVNYG
                     KEWQGINFADGQDPYYNPEKAKAKFAEAKKELEAKGVQFPIHLDKTVEVTDKVGIQGV
                     SSIKQSIESVLGSDNVVIDIQQLTSDEFDSSGYFAQTAAQKDYDLYHGGWGPDYQDPS
                     TYLDIFNTNSGGVLQNLGLEPGEANDKAKEVGLDVYTQMLEEANKEQDPAKRYEKYAD
                     IQAWLIDSSLVLPSVSRGGTPSLRRTVPFAAAYGLTGTKGVESYKYLKVQDKIVTTDE
                     YAKAREKWLKEKEESNKKAQEELAKHVK"
     gene            complement(15059..16681)
                     /locus_tag="VYN69_000673"
     CDS             complement(15059..16681)
                     /locus_tag="VYN69_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1532985.1"
                     /translation="MSHENNHQQAQMLRGTAWLTASNFISRLLGAVYIIPWYIWMGAY
                     AAKANGLFTMGYNIYAWFLLVSTAGIPVAVAKQVAKYNTMREEEHSFALIRSFLGFMT
                     GLGLVFALVLYVFAPWLADLSGVGKDLIPIMQSLAWGVLIFPSMSVIRGFFQGMNNLK
                     PYAMSQIAEQVIRVIWMLLATFIIMKLGSGDYLAAVTQSTFAAFVGMVASFAVLIYFL
                     AQEGSLKRVFETGDKINSKRLLVDTIKEAIPFILTGSAIQLFQILDQLTFINSMSWFT
                     NYSNEDLVVMFSYFSANPNKITMILISVGVSIGSVGLPLLTENYVKGDLKAASRLVQD
                     SLTLLFMFLLPATVGVVMVGEPLYTVFYGKPDSLALGLFVFAVLQSIILGLYMVLSPM
                     LQAMFRNRKAVLYFIYGSIAKLVLQLPTIALFHSYGPLISTTIALIIPNVLMYRDICK
                     VTGVKRKVILKRTILISLLTLVMFLLIGTIQWLLGFFFQPSGRLWSFFYVALVGAMGG
                     GLYMVMSLRTYLLDKVIGKAQADRLRAKFKLS"
     gene            16787..18232
                     /locus_tag="VYN69_000674"
     CDS             16787..18232
                     /locus_tag="VYN69_000674"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEV1532986.1"
                     /translation="MIKIETVLDILKKDGLFREIIDQGHYHYNYSKVVFDSISYDSRK
                     VTEDTLFFAKGAAFKKEYLLSAITQGLAWYVAEKDYEVGIPVIIVNDIKKAMSLIAME
                     FYGNPQEKLKLLAFTGTKGKTTAAYFAYNILSQGHRPAMLSTMNTTLDGETFFKSALT
                     TPESIDLFDMMNQAVQNDRTHLIMEVSSQSYLVRRVYGLTFDVGVFLNISPDHIGPIE
                     HPSFEDYFYHKRLLMENSRAVIINSDMDHFSVLKEQVEDQDHDFYGSQFDNQIENSKA
                     FSFSATGKLAGDYDIQLIGNFNQENAVAAGLACLRLGASLEDIKKGIAATRVPGRMEV
                     LTQKNGAKVFIDYAHNGDSLKKLINVVETHQTGKIALVLGSTGNKGESRRKDFGLLLN
                     QHPEIQVFLTADDPNYEDPMAIADEISSYINHPVEKIADRQEAIKAAMAITNHELDAV
                     IIAGKGADCYQIIQGKKESYPGDTAVAENYL"
     gene            complement(18275..18472)
                     /locus_tag="VYN69_000675"
     CDS             complement(18275..18472)
                     /locus_tag="VYN69_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1532987.1"
                     /translation="MSLENKLEQATGAVKEGFGKVTGDSKTELEGAVEKTVAKAKDVV
                     EDAKGAVEGAVEGLKNVFTKE"
     gene            complement(18573..19181)
                     /locus_tag="VYN69_000676"
     CDS             complement(18573..19181)
                     /locus_tag="VYN69_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEV1532988.1"
                     /translation="MFGELIRYLDQYEDVILREIKAQFPDVAVDKLMEEYIKAGLILR
                     ENKRYYLNFPTLESLDSLELDQEIFVREASPVYQALLEQSFETELRNQINAAILVEKT
                     DFARIKMTLSNYFYKVKQQYPLTEKQQELYDILGDVNPEYALKYMTAFLLKFLKKDQL
                     MQKCRDIFVDSLVVLGYIVQNEDGKYELAIDFDKERLTFYLA"
     gene            complement(19206..19718)
                     /locus_tag="VYN69_000677"
     CDS             complement(19206..19718)
                     /locus_tag="VYN69_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000892254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HEV1532989.1"
                     /translation="MLENGDLIFVRDGSDMGQAIQTSTGNYSHVAIYLDGMIYHASGQ
                     AGVVCQEPADFFESNHLYDLYVYPEMDIQSVKERACKHLGAPYNASFYPDAPGFYCSQ
                     YIAEILPIFETIPMKFGDGEQEISDFWREYYIELGLPVPLNQAGTNPSQLAASPLLQC
                     KERNLHDSDF"
     gene            complement(19805..20740)
                     /locus_tag="VYN69_000678"
     CDS             complement(19805..20740)
                     /locus_tag="VYN69_000678"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEV1532990.1"
                     /translation="MSKILVFGHQNPDSDAIGSSVAFAYLAKEAYGLDTEAVALGTPN
                     EETAFVLNYFGVEAPRVITSAKAEGAEQVILTDHNEFQQSVSDIAEVEVYGVVDHHRV
                     ANFETASPLYMRLEPVGSASSIVYRMFKEHGVAVPKEIAGLMLSGLISDTLLLKSPTT
                     HPTDKIIAPELAELAGVNLEEYGLAMLKAGTNLASKSAEELIDIDAKTFELNGNNVRV
                     AQVNTVDIAEVLERQAEIEAAMQAANESNGYSDFVLMITDIVNSNSEILALGANMDKV
                     EAAFNFKLENNHAFLAGAVSRKKQVVPQLTESFNA"
     gene            complement(20811..21110)
                     /locus_tag="VYN69_000679"
     CDS             complement(20811..21110)
                     /locus_tag="VYN69_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEV1532991.1"
                     /translation="MDHKAYMYVLECRDGSYYIGYTTDVRRRLAIHNSGKGAKYTRAR
                     LPVKLIYAQGFASKEEAMSAEALLKRKKRPQKEEFLSENQDRNLLSYFEESWGVL"
     gene            complement(21100..21849)
                     /locus_tag="VYN69_000680"
     CDS             complement(21100..21849)
                     /locus_tag="VYN69_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEV1532992.1"
                     /translation="MEEEQLLKSGERINQLFSTDIKIIQNREVFSYSVDSVLLSRFPR
                     FPKKGLIVDFCAGNGAVGLFASTRTQAQILSVEIQERLADMAERSVRLNGLEEQMQVI
                     CDDLKNMPAHIQGSKVDMILCNPPYFKVDPYSNLNESEHYLLARHEITTNLEEICRSA
                     QSILKSNGRLAMVHRPDRLLDILDTLKRHNLAPKRLQFVYPKREKEANMLLIEAIKDG
                     STSGFKVLPPLIVHNDDGSYTPEIEEIYYGS"
     gene            complement(21903..22262)
                     /locus_tag="VYN69_000681"
     CDS             complement(21903..22262)
                     /locus_tag="VYN69_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEV1532993.1"
                     /translation="MKIGDKLKGRITGIQPYGAFVELETGDTGLIHISEIRTGFIENI
                     HETLKVDEEVQVQVVDLDEFTGKASLSIRTLEEEKYQFPRRRRFSSDRFNYGFAPFRR
                     MLPIWTGEALHHLKKKK"
     gene            complement(22264..23664)
                     /locus_tag="VYN69_000682"
     CDS             complement(22264..23664)
                     /locus_tag="VYN69_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional Cof-type HAD-IIB family
                     hydrolase/peptidylprolyl isomerase"
                     /protein_id="HEV1532994.1"
                     /translation="MDAKLRYKAKKIKIVFFDIDDTLRNSKTGFIPTTIPTVFKQLRE
                     KGILTGIASGRGIFGVVPEIRDLKPDFFVTLNGAYIEDKKGQVIYQHQIEKSDVEEYI
                     SWAKQEGIEYGLVGSHDAKLSTRTDMMSEAINPIYPDLDVDPDFHEKEDIYQMWTFED
                     KGDDLHLPDSLSDKLRMVRWHQHSSDIVPISGSKATGVEKVVEHLGLKPEKVMVFGDG
                     LNDLELFDYAGISVAMGISHDKIKEKADYITKTLEEDGIFAALEVFGMVEKELHFPQV
                     DIETVEGPLATIKTNHGDLRIKLFPEHAPKTVANFVSLSKDGYYDGVIFHRIIKDFMI
                     QGGDPTGTGMGGESIYGESFEDEFSEELYNIRGALSMANAGPNTNGSQFFIVQNQHLP
                     YSKKEITRGGWPEPIAEIYANQGGTPHLDRRHTVFGQLADEASYAVLDAIAAVETGAM
                     DKPVEDVVIETIEIED"
     gene            complement(23959..24198)
                     /locus_tag="VYN69_000683"
     CDS             complement(23959..24198)
                     /locus_tag="VYN69_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEV1532995.1"
                     /translation="MAQQRRGGFKRRKKVDYIAANKIEYVDYKDTELLSRFVSERGKI
                     LPRRVTGTSAKNQRKVTTAIKRARVMALMPFVNED"
     gene            complement(24230..24700)
                     /gene="ssbA"
                     /locus_tag="VYN69_000684"
     CDS             complement(24230..24700)
                     /gene="ssbA"
                     /locus_tag="VYN69_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SsbA"
                     /protein_id="HEV1532996.1"
                     /translation="MINNVVLVGRMTRDAELRYTPSNVAVATFTLAVNRTFKSQNGER
                     EADFINVVMWRQQAENLANWAKKGSLIGVTGRIQTRSYDNQQGQRVYVTEVVAENFQM
                     LESRSVREGHTGGAYSAPTANYSAPTNSVPDFSRNENPFGATNPLDISDDDLPF"
     gene            complement(24712..25002)
                     /locus_tag="VYN69_000685"
     CDS             complement(24712..25002)
                     /locus_tag="VYN69_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018030687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEV1532997.1"
                     /translation="MAKYEILYIIRPNIEEEAKNALVARFDSILTDNGATVVESKTWE
                     KRRLAYEIQDFREGLYHIVNVEANDDAALKEFDRLSKINADILRHMIVKIDA"
     gene            complement(25155..26501)
                     /gene="asnS"
                     /locus_tag="VYN69_000686"
     CDS             complement(25155..26501)
                     /gene="asnS"
                     /locus_tag="VYN69_000686"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEV1532998.1"
                     /translation="MMTKRVTIIDVKDYVGQEVTIGAWVANKSGKGKIAFLQLRDGTA
                     FFQGVAFKPNFVEKFGEEVGLEKFDVIKRLSQETSVYVTGIVKEDERSKFGYELDITD
                     IEVIGESQDYPITPKEHGTDFLMDNRHLWLRSRKQVAVLQIRNAIIYATYEFFDKNGF
                     MKFDSPILSGNAAEDSTELFETDYFGTPAYLSQSGQLYLEAGAMALGRVFDFGPVFRA
                     EKSKTRRHLTEFWMMDAEYSYLTHDESLDLQEAYVKALLQGVLDRAPQALETLERDTE
                     LLKRYIAEPFKRITYDQAIDLLQEHENDEDADYEHLEHGDDFGSPHETWISNHFGVPT
                     FVMNYPAAIKAFYMKPVPGNPERVLCADLLAPEGYGEIIGGSMREEDYDALVAKMDEL
                     GMDRTEYEFYLDLRKYGTVPHGGFGIGIERMVTFAAGTKHIREAIPFPRMLHRIKP"
     gene            complement(26517..26882)
                     /locus_tag="VYN69_000687"
     CDS             complement(26517..26882)
                     /locus_tag="VYN69_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1532999.1"
                     /translation="MIDHFEIKVKDLQISEGFYRSFLAPLDYKMAFKTSSLISFLSPN
                     SPHPGGDFWLTQGTQDPVHFAFLAENKEEVQACYEAGIEAGGRDNGVPGYRSEHPIYY
                     AAFMIDLDGNNIEVVCHKE"
     gene            complement(26875..28062)
                     /locus_tag="VYN69_000688"
     CDS             complement(26875..28062)
                     /locus_tag="VYN69_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1533000.1"
                     /translation="MKLSNRVLEMEESVTLAAGARAKALKAEGRDILSLTLGEPDFTT
                     PKNIQDAAIASIRDGRASFYTVTSGLPELKAAVNSYFERFYGYSVASNQVTVAAGAKY
                     SLYTFFMAVVNPGDEVIIPTPYWVSYGDQVKMAEGVPVFVSAKEDNHFKVTVEQLEAA
                     RTDKTKVLVLNSPSNPTGMIYTREELLAIGNWAVENDILILADDIYGRLVYNGHEFTP
                     ISSLSEAIRKQTVVINGVSKTYAMTGWRIGYAVGEADIIAAMSKIAGQTTSNPSAVAQ
                     YAAVEALSGEQDTVESMRQAFEERLNTIYPLLAEVPGFEVVKPQGAFYLFPNVKKAME
                     MKGYTDVIDFTTAILEEAEVALVTGAGFGAPENVRLSYATDLDTLKEAVERLKAFMGS
                     END"
     gene            complement(28059..28547)
                     /locus_tag="VYN69_000689"
     CDS             complement(28059..28547)
                     /locus_tag="VYN69_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEV1533001.1"
                     /translation="MKKRQNKAKNNLLWQYGLGMTILFVVISGSFLYMVSLSMKPYQT
                     AKSEGEKLAQQYAGLEQAVQVDLYNGLESYYSVLGRNKQQEALAVLIGKDDHKIYVYQ
                     LNQGVSQEKAETVSKEKGAGEIDKITFGRYQDKPIWEVKSGSDFYLVDFETGALVNKE
                     GL"
     gene            28868..29416
                     /locus_tag="VYN69_000690"
     CDS             28868..29416
                     /locus_tag="VYN69_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1533002.1"
                     /translation="MSKKVLFIVGSLRQGSFNHQMALEAEKALAGKAEVSYLDYSALP
                     LFSQDLEVPTHPAVAAAREAVLVADAIWIFSPVYNFSIPGTVKNLLDWLSRALDLSDT
                     RGVSALQDKFVTVSSVANAGHDQLFAIYKDLLPFIRTQGVGDFTAARVNDSAWADGKL
                     VLEETVLNSLEKQAQDLVEAIK"
     gene            complement(29693..30421)
                     /gene="yaaA"
                     /locus_tag="VYN69_000691"
     CDS             complement(29693..30421)
                     /gene="yaaA"
                     /locus_tag="VYN69_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HEV1533003.1"
                     /translation="MKILIPTAKEMNTDLPSIEAISLKPESQAVLDALALYSANQLES
                     FYKVSAEKAAEEFQNIQALKRQTTQHYPALKLFDGLMYRNIKRDKLTEAEQDYLENHV
                     FITSALYGVVPALSPMAPHRLDFLMKLKVAGKTLKSHWKAVYDEALKKEEVIFSLLSS
                     EFETVFSKEIRAKMVTFKFMEDRGGQLKIHSTISKKARGAFLTALIENQVQTVGEARR
                     LNFAGFVYREDLSQPQGLVFVKEV"
     gene            complement(30500..30664)
                     /locus_tag="VYN69_000692"
     CDS             complement(30500..30664)
                     /locus_tag="VYN69_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YczI family protein"
                     /protein_id="HEV1533004.1"
                     /translation="MGFYLMVASMLLGLLALKIGFSQFKEKKDKFLSILTSLAGTALV
                     LVAVWLGWPK"
     gene            complement(30731..32326)
                     /locus_tag="VYN69_000693"
     CDS             complement(30731..32326)
                     /locus_tag="VYN69_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000822862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533005.1"
                     /translation="MKRIVFELIFIATTWYIFLPPLNLTSWEFLFFLCGHLLVVAILF
                     GFGKGINLVKTVHVRHGKAEAALNLEGFKINRLGKILLASIGGILLLAALVSLVTSSM
                     FQAKNYANVVTVTEKDFTEFPRSDTSKVPILDRSTAEKIGDRYLGSLTDKVSQYVAAD
                     TYTQLTIDGKPYRVTPLEYADPIKWFNNQAKGIGEYIKVDMVTGNADLVDLKTPIKYS
                     DSEYFNRDVKRHLRLKYPTKIFKTPSFEVDDEGNPFYVATVYQKQFGLAVPRPASVII
                     LDATNGETKEYSLSDVPEWVDRIYPAEETIEQINYNGKYKDGFLNAMISKKNVTQTTK
                     GYNYLSIGNDIYLYTGVTSANADESNLGFILENMRTGEITKYSLASATEESARESAEG
                     AVQEKSYKATFPILINLNDKPLYIMGLKDNAGLVKEYALVDAVEYQNVIVATTVEEML
                     SKYANKNDLEIDNATTESINGVVADLKSAVIKGDTVYFFKVDGKIYKVKASVSDDLPY
                     LENGKTFEGQVGKDNYLKTFKLR"
     gene            complement(32338..32748)
                     /locus_tag="VYN69_000694"
     CDS             complement(32338..32748)
                     /locus_tag="VYN69_000694"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000412213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1533006.1"
                     /translation="MEKKIVKDILFLSQVSQPASQEDLYLARDLQDTLLANHDTCVGL
                     AANMIGVQKRVIIFNLGLVPVVMFNPVLLSFEGSYEAEEGCLSLVGVRSTKRYETIRV
                     AYRDSKWQEQTITLTGFPAQICQHELDHLEGRII"
BASE COUNT         9471 a         7416 c         6137 g         9849 t
ORIGIN      
        1 agctcaaaac actgttttga ggttgtaaat gaaactgaca aagtcagtaa ccatacatac
       61 ggcaaggcga cgctgacgtg gtttgaagag attttcgaag aatagcaaaa tggaaaaagg
      121 agtgagtgaa gcacatcgcc tccccactcc tttttctgtt tttaggctgt tttttcaacc
      181 ttcaagattt ttacatcata gctaccaaca ggcgtttcaa tggttgctgt gtcacctgtt
      241 ttcttgccaa tcaaggcctg cccaattggg ctttcatttg aaaccttacc tgcaaaggca
      301 tccgcaccag ctgaacctac gataatataa acttcttctt cgtcctcacc aatttcttgg
      361 atggtgactg ttttaccaat cgctacttcg tcctgggcaa ctgcgtcgct attgacgatt
      421 tcagcatagc ggatttttgt ttctaagcta gagatttgtc cttcgacaaa ggcttgttca
      481 tccttagctg cttcgtactc actgttttct gaaagatcac cgtatgaacg ggcaatctta
      541 atgcgttcta ccacttctgg tcgacgaacc aatttcaatt cttctaattc tttttcaagt
      601 ttctcctttt cctcaagggt cataggatat gttttttctg ccatttttct caactttctt
      661 ctgataatat tttctaaaga aaattatgtg aagtatcaca taattttagt ttgtttagtt
      721 taatttgctg ttgacatgtt cagcgacatt gcggtcgtgg tcttcttgat tgttagcata
      781 gtagaccttg ccttctgtga catctgctac aaagtagagg ttatcgctct tagtttgatt
      841 gatgcttgac tcaatcgcat ccagacttgg actatcgact ggaccaggca tgagacctac
      901 atttttataa acattataag gtgaatcaat gttggtatca atcgcaacat cctcagctag
      961 actgatattt tgccccagtt ttccttgggc atacaagatt gcaatattac tttgaagtgg
     1021 catatcacga ttcaaacgat tgtagaatac acctgcaatg agcttacgat cttctgtctt
     1081 ggcaccttct ttttcgacca aggaagcaat ggtcaacaac tcattgacag tcaagttttt
     1141 agatttgata gtactatagt aaggagatag gttcttatcc atagcagcta acatctcatc
     1201 aatcaagctc tcaatagttg tgctttcctt gatagagtat gtagctggga aaaggtatcc
     1261 ttccaaacga taacgcgcac cgctgtcttt tacaggcaaa ctttccagta aagtaggata
     1321 tttcgctact gcttgactga taaacgtctc atcttgaact ttagccaaga aagcctccgc
     1381 tgtcaaagac tctttgaagt caccttgcaa ttgacccaca gtttgagcaa tctgatccaa
     1441 ggtataacct tctggaattg tcaaagtcgc aagtacaggt tcttgcggtt catctgttcc
     1501 acctttttgc aactctttga gtaagtcttc tgtactcata ctcttttgca aattgtagta
     1561 acctgctttc aagtcggtat aatttttata cttggcataa aaactaaaaa tcagaccatg
     1621 ctttaccaaa ccagcttttt caagcgtcgt accgatttct tgaacgtttg aaccttctgg
     1681 aattccaacc gtcacatatt tcttagaatt agcatcgata ggtaataagg aatctagcac
     1741 gtactggtaa ccaaagtaac cacctgctga gagcaaggca aggaatacaa ggaatgaaat
     1801 cacaaaagct ttcacatgtg acttcttctc tttcttaggc ttaacgggtt ttgctccttc
     1861 tctacggcta cgacgtgatg tatctctgat gatatccact gtttctgtag caggcgtagg
     1921 tgtttctgga cctgcttgtt ctgctttttt ctctgccgtc gtataggaaa cagctaccct
     1981 tgttggggtt tcattgtatt ctctttcaag tttcttaggt ctaacaggac ctggacctgg
     2041 tcttgatcca ctttcttccg ctggagaaga aggtacatct tgacttggat gacttggaac
     2101 accatgagtt tctctttgaa tctcatctgc tggagaagct ggtacacctt gacttgggtg
     2161 agtaggcacg gtaggagctt ttctcataat ctcctctacc gttgacaagg aatcagccat
     2221 gagttcttca gctgaaggtt catttgcagg agtgcgaact actgcctcat cttctttcag
     2281 aacttcatca tagcctttta ctttttctaa atctctcaga atctgctctt taaagcttaa
     2341 tttctcttct tctcttgact tttcactcaa aagtttttcc tccttgttga taatccataa
     2401 tattatatct taaaagtcca aaaaaagcaa tctatgatac ttttcctaac ttattttttc
     2461 atttcccaga ccatatcata ccatgtttcc cctgcaaagg ttgactggga ctggccttcg
     2521 ttgacaaatc catgtttttc ataataggcg atgagatagt catgacaggt tagattaatg
     2581 ccatctctct catcttcaag agcaacttct ttcaaggctg tcagcaattt ctgacccagt
     2641 ccgagtccct gtgcctcctt ggcaatagac agacaggtca cagagatata accaccaggc
     2701 tcatgactat agtcttttat ttcttctgta aaagactggt cttgcagatg gcggtgcagg
     2761 ccaactggtc cttcgatata acccatgatt cttccttctt tttcagcaac cagaaaagag
     2821 gtctgaattt ctctcaaatg tgcttcaaag acagaaggag gaatggcttc ttcgaccgaa
     2881 aaattttcaa attcaagttc aacaatccga tccaaatctt ctaatcttgc ttgtctgatt
     2941 ttcattgttc ctccagataa aagggattaa accaaatcat actatagccc tggctagttg
     3001 catagagcaa aatttcttct tcatcaacaa aaccgttcat ttcaaaatag gaaagcagct
     3061 catcaggact ctccaaacga atccctttgt aatccagctc aactgccacc tctttcaagg
     3121 ctgcaagaag aagtgttccc aggccctgtc tctgatggtc agactcgatg actaaagaat
     3181 gtacttttag acattgcgga ttgtctgact ggggacttga taaaatatag cctaaaagtt
     3241 gattttcatc cctagctaga agaaaggtat ccgcacactt acggatactt tcttctaaaa
     3301 tatgggaaag ttgctgcttt tcagctggaa aagacgaggt ctgaagtgcc cctatctcag
     3361 gcaaatcaaa cttgcttgcc tgaatgatct taattggaat ttccatggga acatcctatt
     3421 gaacattgct tgtcaagtta gacaagagac gctcaaatga gtattcatag gtttggatgt
     3481 ctcctgctcc cataaagacg taaacagcat tgtcatggtc taggagtgga gaaacatttt
     3541 caacagtaat cacttggtgt tttttgttga ttttattggc taggtcttct accttaacgt
     3601 caccatgatc tacttcacga gccgagccat aaatttgcgc tagataaaca gcatctgctt
     3661 ggtttaaagc atgggcaaag tcgtccaaca gggcaatggt tcttgtaaag gtatgcggtt
     3721 gaaagactgc tacaatttcc ttgcttgggt atttctgacg agccgcatcc aaggtcgcaa
     3781 taatttctgt tggatgatgg gcaaagtcat caataatcac tgtatcattg acaattttct
     3841 cagtgaaacg acgtttaaca ccggcaaatg ttttcaagtg ctcacgcacc aagttcaaat
     3901 caaatcctgc tgtgtaaaga agaccaataa cggctgtcgc attcatgata ttgtgacgac
     3961 caaaggttgg aatgtggaat tgccccaagt tttgtccacg gaaatgaacg gtgaaggttg
     4021 aaccagttgt tgaacgaaga agatcactag ctacaaagtc attgccttca gcttcaaaac
     4081 cataataata aattggtgca tcagacgtaa tcttacgcaa ttcagcatct tcaccataga
     4141 caaaaagacc cttggtaatt tgtttggcat agtcgttaaa ggcattgaaa acatcctcga
     4201 gacttgtgaa ataatctgga tggtcaaagt caatgttggt gataatagag tattctgggt
     4261 ggtaaggcat gaagtgacgc tcatattcgt cagattcaaa gacaaaatat ttggcattag
     4321 ccgaaccacg acctgtccca tctccaatca agaagctggt atctgtaatg tgagacaaga
     4381 catgagacaa catacctgtc gttgaagttt ttccatgtgc tcctgctact cccatgctaa
     4441 caaagtcacg cataaagcta cctagaaact catggtaacg tttgtagctg ataccatttt
     4501 ggtccgcata ggcaatttcg acgttgttat ctggacgaaa ggcatttcca gcgataattt
     4561 ccatatcacc gtctagattc ttttcatcaa aaggaagaat ggtaattcct gcctgctcaa
     4621 gaccgcgttg ggtaaagtag tacttttcaa catctgatcc ctgaaccttg tgccccatct
     4681 ggtgcaacat caaggccaag gcactcatcc ctgatccctt aattccgata aaatgatatg
     4741 tctttgacat gttttctccc ctattctgtc attctggtca gattcaactc ttgggcaacc
     4801 cgacgttctt gttctgtttg tttacttttt ttattgtaga tttggctctt ctttagaaaa
     4861 tcataattgt ttttctttgg agcaggtgct gacacttctt cattcttggt agggatagaa
     4921 tgaacttctt ccgccaagat ataatgagac tgggtcaatt tttggctata tttgacaaat
     4981 tcaccaggat tttccttttg gaaaggagtt gtcggttgat tgccctgtct aactagactg
     5041 ggctgagaat gacgtctcgc aaggctgaaa tcctgagtta ggtagttagc agagcgtttc
     5101 tttttcaagt ccgcacgcgc ttcttcacgc gccacctccg catagctctt tccttctttt
     5161 ttaaccccta aaggagcctt tttaggtttt tcgacttgct tttcaatcgg ttttactggt
     5221 ttttcttcag taataggagc ccattctaaa taatttttat ctcgatactc acccttgata
     5281 ttactgatca gatcagactc atcatagaga ttcatgactg gcatttcagt caacatgacc
     5341 tcgtcatctg acaccaatgg aaatcgttct tgtttcattt tctatttcct ttcaacactt
     5401 cattatagcg tattgtcttg atttttcaag tgctggcttc agaaattccc aaaatttctc
     5461 taatttctgc tagggtcaga ctaccacgtg actctgtgcc atccaatact tgtgacacca
     5521 gatgtttctt ttgttcttgg agttcctgaa ttttttcttc aatggttccc ttggtcacca
     5581 agcgatagac ctcaaccgtt tcttcctgac ccatccgatg ggcacggcca atggcttgcg
     5641 cttccaccgc aggattccac caaaggtcaa ccaagatcac tgtatctgca cctgtcaggt
     5701 tcagaccgac cccaccagcc ttgagggaaa tcagaaaggc atctctttct ccttggttaa
     5761 aggccttggt catgtcttgt ctttccttgg ctggggttga acccgtaatt ttaaaggatg
     5821 tcaggcccaa gtctggcagt tcttgttcaa ttttttccaa cattcccttg aactgagaga
     5881 aaatcaagac acggtgtccg ccgtctgcca cctgtaccag taggtctcgg agactatcta
     5941 gtttgccgct ggctccctga taatcttcca taaacagggc aggagtgtca catatttgac
     6001 gcaagcgcat caaaccagat aaaatttcca cacgacttcg ctgaaattcc tgttctgaca
     6061 cttgagccag atggtctcgc atctgttgta actgggcaag gtaaatagcc ttttgctggt
     6121 cttccagttc atttttataa accacctcaa tcaagtctgg caattcagtc agaacttctt
     6181 ctttcttgcg tcgcatcacg aaaggcttga taaactgagc cactcgctct gctggcaatt
     6241 tcataaattc tttcttgctt ggcaaaagtc cgggcatgac gatttggaaa atagaccaca
     6301 actcacccag atggttttca atcggagttc ctgacaaggc aaagaccgac ggcaccacaa
     6361 attgtctcaa ggtctgggca atcttggtct gggcattttt catgacctga gcctcatcta
     6421 agaaaaggaa gtcaaaggcc atcccctgat aaaactcact gtcctgacgg aaggtggcat
     6481 agctagtcac atagatttga tggctctcgg caagaatctc ctcacgactt gctttcaaac
     6541 catgaacaac agccacatcc aactgtgggg caaatttctg aaactcatcc gcccagttgt
     6601 aaatcaaacc cgacggagcg agaatcaaga ctcgactttc ttttgtcact tgactagtca
     6661 aaaaagcaat ggtctgaagg gttttcccaa gtcccatatc atcagccaaa atcccaccaa
     6721 aaccataatg atggagcatc tgcaaccagc caattccctt ttcctgataa tctcgcaagt
     6781 cagccttgac ctgagttgct tgcaaaggaa agtcctctgg atgcgtcaaa tcctgggcca
     6841 gattctggaa ttcttgtgaa aaagaaacac ggtctcgccc ttcaaagaga tgagctaaac
     6901 tgtaggccaa ggatttccga gcctgcaagg tcccatcttt taattcaaat tgccccagtt
     6961 cctgtagatt ttggcgaatt ttcttggttt cttcatcgaa aaagtaaact tgattagacg
     7021 aatcaatata aaaatcctga ttggcaacca aggcctgcat ggcttggtcg atttcctcct
     7081 gggcaatatc ttgaaaatca aactggattt ccaagagacc tcccttggag gcaatttgca
     7141 cctgaggact cgctaggcta taaagctctt ctagtttatc tgataggtca acatgcccga
     7201 gtttttcaaa gactggaatg atatcatgaa aaaaatgata gacagactcc gcttttaagg
     7261 cctgacgcca agattgaaaa tcggcttcaa agcccgcagc caaacagact tggaaaattc
     7321 tttcttctaa gtccgcgtca cttgaaaagg gtaattcttc tagctcttgt cggctagata
     7381 cctgtctatt tccataatca aactgaattt ctaaacgaat ccgattatct tcttccctgt
     7441 caaagtaaaa agagggcgca aaagttttga tttgtagacg ttctggagct gaaacggtgc
     7501 ccatctggat aaaaagagtc agacaggagg ccaatttgtc tcgatcactg ctatcaaatt
     7561 gcaggtattt ctttccttgt tgacccacag gtaacacttt aatttccttg agaagacgca
     7621 tctgctggtc tgttaaaaaa taaacctgac ctttatggaa aagtactgct ccctgataaa
     7681 agacattaac cctaggactc tcactgattt ccatttcaaa ataatccgag tattctgtta
     7741 ctgtaaaggc aaatagattg gcatcagcat gcatatcctg aaaaagcagg gtttggtagc
     7801 tatccacttg atggtcaaat tgaaaatggg gcaaggccat cagtaaattc acaccctgct
     7861 caaaaaaggt cagagggaaa aagaggtgcc gaccttggtt ttggaaaaag aggtctggaa
     7921 ccagcccttc ctccgttagt ccgtgcaaga aagtcaaaag ttcttggctg gcatcatcaa
     7981 aggcttccca agaaagagac tcctcataaa tcttgccaat catatacgac tttctctgct
     8041 cgacaatcct taaaaaaagt ggaatatccc gaatgacata gtatttttgg ctattgattt
     8101 ggccgattct cagagtccac aagatatgat tggttcctgc ttccacctga cccacagctg
     8161 ataactcata ggcgcattct gattttggag ataaaattcg atccaaaaac ttgccaccca
     8221 aggtcacctt ggtttcaaca gcctcttttt cttcatgacc ttcttccaga ctccacaaga
     8281 tttcctgacc acgctcatca tttttcagaa aatgctctag cgctgccaaa tgcacacagt
     8341 agcccctctt ttgaaaaaaa tcacaggcac aaaaaaccaa atcatcctct aaactatagc
     8401 gcagttcttc ttctgcaacg cgagcgtaga gccgattgtt cttttccttg atgatatcaa
     8461 ccttaccagt ttcataaagg gcaacacctt cgatacgaat tttccccgga atcaatttag
     8521 ccatattttc acctttacct tatcttttta ttataccata ttttcgccta tgaaaatagc
     8581 cttctaggaa gacttttctc ctagaaggct ggatttttaa cgtttggcaa aagtagccac
     8641 aatccgctga cagacttctt gcaacagaga tttgggcata gctatattga tgcgggcatg
     8701 gagacttcct tcctctccaa aatccaaacc acggttgagg ataaccttgg cttcatttct
     8761 caacaactct tgcaatgttt catcagtcag gtcataagct gaaaagtcaa gccaaatcaa
     8821 gtaggtacct tgcggtttca tgaccttgat tttagtctct tttccaaata gatccatcac
     8881 ataattgatg tggtcttcaa agacttgctt gagttcctct agccaatctt taccgtatcg
     8941 ataggcagct tctgtcgcca aataacccaa gcctgaaatt tcatgctgat tattggccaa
     9001 caggcgtttc tggaaagcca gtctcaactt aggattttca atgactgcat aggaattttt
     9061 tgttccagca atattaaatg ttttagtggc actgctcaag acgatagcaa aatttttgaa
     9121 ggcaggattg atggtattga aagactggtg tttgtgacca aagagggtca aatcttggtg
     9181 aatctcatcc gaaactaaca aaacaccgtg tttttggcag agttggccaa tcttctccaa
     9241 cacttctttt tcccaaacac gtccaccagg attgtgaggg ttgcaaagaa catagagttt
     9301 aacctcctct tccaccaaat ccttttcaag ttggtcaaag tcaatctcaa acagactatc
     9361 cttttccact aaggaattag taatcaatct acgattattc aacttgacac tgcgagcaaa
     9421 gggtgggtag acaggcgtgt taattaaaac cgcctcgcct tcttttgtaa aggtttgaat
     9481 agctgttgag atggctggta ccacaccctc gataaagaca agagcctctt tgtcaaagtt
     9541 gtaaccgtat tgtgtagctt cccacttttg aacttcctta attaagtctt cactggcata
     9601 ggtataacca taaaccagtt ggtctgcgta agtttgcacg gcttggcgga tttcaggcaa
     9661 gaccacaaag tccatatccg ctatccaagc tggtagaact tcactatccg tttctgtttc
     9721 tttccattta taggtatggt gccctaaacg gttgggcagg cttgtaaaat catattttcc
     9781 catctttgtc ttatccttct atggcttggc gcaaatctgc aatcaaatct ctagcatcct
     9841 caatcccaat agacaaacgc aagaggtcat ctgtcaaacc ataagaatgg cgtacctctg
     9901 ctggaatatc agcatgagtt tgagtcgttg gataagtaat aagactttcc actccaccca
     9961 aactttccgc aaaagagaag accttgagac tgttcaaaat atgaggaatg cgtgtttcat
    10021 cggctacttt aaaggaaatc atgcctccac gaccagtgta gagaacttcc ttaactgctg
    10081 gagaatcctt caaaaaggca accacttctt gggcgttagc tgttgagcgc tccatacgaa
    10141 gagacaaggt cttgagacca cgaagcaact ggtagctgtc aaatggagac aagactgccc
    10201 ctgttgtatt aagattgtaa aaaagcttct cgtatagttc taaactattg gtcacaacca
    10261 ctccagccaa gacatcattg tggcctgcta gatacttggt tgctgaatgg agaacgatat
    10321 ctgctccatc ttcaatcgga cgttggtaga tagggctata gaaggtattg tccaccacca
    10381 ctttggcacc cttagcatga gccaattttg ctagtttttc gatatcaaat tccaacatca
    10441 agggattggt tggggtttcg atatagagaa catccacatc cttttctaac tcagcaatca
    10501 actcttcttc tgtattggca taggtaaaat ggaaacgacc ttcctgctcc acttggttaa
    10561 accagcgaaa agaaccaccg taaagatcac gcactgccaa gaccttactt cctactggaa
    10621 agccgctaaa ggccagtaca atagctgaca tccctgagct agtcgctagg gcatagtctg
    10681 ctgactcaat agccgccaag acttcctcag ccttactacg agttggattt ttagtgcgcg
    10741 tatagtcaaa cccagtagat cgaccaaact ctggatgctg ataggtcgtt gaaaaatgaa
    10801 gcggtgtcac caatgcacct gtcgcttcat ctgacttaat acccgcctga gccaaaattg
    10861 tgttaatgtg taattccttg ctcatacaat acctccaaat ctatagtaac tattgtacca
    10921 cttattttca tcaactcatt ttcttctttt caagagctag ttatagtttc aaactatata
    10981 aaaagggagt ttttcctgct ccctttaata gactataaaa tggtgaatct caaaagacac
    11041 cttcactcta tcatttgctc ctgcacaaaa cgagcataac gctcatgatt ttccagtagt
    11101 tccttatgag ttcctgagcc agtgattttc cccccctcta agaagaaaat acaatccaca
    11161 tcttttaccg ttgacaaacg atgcgctata atcacaaccg tcttctcctt taatgcagaa
    11221 tagaggatac tgataatcgc atactcagaa tccgcatcaa gattagcagt ggcttcatca
    11281 aatataagaa tttcagcatc ttttaagtag gttctagcta tttgaagtct ttggctttgc
    11341 ccccctgaca agaatcgtcc gcgttcacca acttcagtat ctagtccctc tttcatggag
    11401 cgaatctcat cacctagtga tactaagtct agcactttca tcaattcatc atcagttact
    11461 aagcgattca aaccgagaca aagattgtca cgaatactgc cagataagac tgcattattt
    11521 tgtgaaaccc aagcgatttt acttctccat tcttttaagt taaaatcata tatacttgat
    11581 tgctccatta gaatatatcc tgaaagcggt ttataaaacc gctctaacaa acgcacaatc
    11641 gttgattttc ctgatccaga tggtccaaca aaagcaattt tttgcccctt gaaaattgaa
    11701 caagtaatat cctttaagac aggtcgattt tcatcataac caaaatagac atggttaaaa
    11761 ttcaaccctt gtcctgatac cgattttcct ccctcaaatt tttctttagg aactgcaagc
    11821 aagttctcca gtgcaactga agatcccttg ctcctagaat aaatagttac aaaattagct
    11881 atattactaa taggattaag taattgaaag aggtaaatca aaaacgaaac caaggttccc
    11941 acagatatat atcctgcgct gacccgataa cccccatagg ttagcatcac aactatagtc
    12001 gcaaagataa ataagagagc aaacggggtc tcaaaagaag taaccctatc tgatttcagt
    12061 gaattgtttt gtaccctttc aatacaatta tccaaaacat cctgtacact tttctctgct
    12121 tggttagtct taattaattc atgttcttga atcttttcag tcaattgccc tgttaaaaat
    12181 ccgatttcct cctgtaaacg acgactatac ttttcactga tattggaaag gggcaagata
    12241 ataaacatca tacaaggaag agtgatgaat aaaagtagag aaagattcca atcaagacta
    12301 aataagacta caatggaacc aagtaccata actaaactca gaataatatt tgggaaagtc
    12361 gtaattaaaa actcacgaat gacactcgtg tcattgacaa tggcagaagt caactcccca
    12421 ctttggctct tatcaaagaa ggatttctct gcataaatca acccctctat cacttttttc
    12481 ctgatttttg ctatcttttt ttcacccgat tgactaaaca gatagtaacc aatagaagaa
    12541 aacaaggctt gaccaataaa aatcaaaaac gattgaaata ctttggaacc tatattttca
    12601 atagaactcc catctattaa accctttaag ataaggggaa gcaacaaagc aagtagacta
    12661 gacagaacaa gtaagaaact ccccataatc accttagtat ctactcttaa taattttaat
    12721 ttcataaata ctccttataa tatttcaacg gataaagtcg agaataactc aatttgagga
    12781 taaaatctaa taaatcttcc tataacaaaa cgcataacat ctaggatttt atatacctga
    12841 tattatgcgt ttttaagcac aaagacttct tacacaaact tatctacaat tagattttat
    12901 ttgacatgtt ttgccaattc ttcttgggct tttttattgg attcttcttt ttctttcaac
    12961 catttttctc tggcttttgc atattcgtct gttgtgacaa tcttatcttg tactttgagg
    13021 tatttatatg attcaacccc ttttgtaccg gttaaaccat aggcagcagc aaatggtacg
    13081 gttcttctca atgatggtgt tcccccacgc gaaacacttg gaagaactaa agaactatca
    13141 atcaaccaag cttgaatatc agcatatttc tcataacgtt tggctggatc ttgctcttta
    13201 ttagcttctt ccaacatttg agtatagaca tccagtccaa cttccttagc cttgtcattg
    13261 gcttctccag gctctagtcc aagattttgt agaactcctc cactattagt attaaaaata
    13321 tcgagatagg ttgacgggtc ttgataatca ggtccccaac cgccatgata taaatcatag
    13381 tctttctgag cagctgtttg agcaaagtag cctgaactgt caaactcatc tgatgttaat
    13441 tgctgaatgt caatcactac attatcagaa cctaaaacag attcaattga ttgtttgata
    13501 gaactaactc cttgtatgcc tactttatct gttacttcca cagtcttatc caagtggatt
    13561 gggaattgaa caccctttgc ttcgagttct ttcttagctt ccgcaaactt agccttggct
    13621 ttctcaggat tgtagtaagg gtcttgacca tccgcaaagt tgataccttg ccattcctta
    13681 ccatagttga ccatcttaga ggctacaact tcaccaaagt cttttccctt gatactgaca
    13741 aagtttggag gaaccactag gttacgcaaa atctttgttg caccttcttt cccttcagac
    13801 tgagccccat aagatgttct gtcaaaagca aaattgatag cctgacggaa gtttttattg
    13861 agaactgctt cctgagtcga tttcttttca atgtcacttg ttttagaagt ataattgtaa
    13921 gacttcctat ctaggttaaa attaaagaaa tatgaagttg aattttgcat actatagatg
    13981 atattgtttt tgtatttttc tttaatccct tcatagctgg agctgttagg aaaaagacga
    14041 gccgtagtat aagcaccagc tgtaaaatta cgttccagtg attcttggtc gctaccatca
    14101 tagtaggtca atttcacatc gtctacaaag acattcttag catcccagta attagggttt
    14161 ttcttatatt caatagcaga ttttgagaca agtgctttca tcaagaaagg tccattgtac
    14221 aaaatactag atggatccgc cttcccaaaa tcatcccctt ttgatttcag gaaatctgca
    14281 ttaacaggaa aaagtatcgt tgcaagtgtt tttgaattcc agtaaagttc tggtttaacc
    14341 aaagtatatt gaaccgtttg gtcatcaagt gccttgacac cgacagttga aaagtcgctt
    14401 gttttaccag tgatatagtc atccaaacca gcaacagagt cctgcactag atacaaggct
    14461 tctgattttt tatcagctgc atattgcaaa cctgtcacaa aatcctgggc agttacaggc
    14521 gcatattctt ctccctcaga agtaaaccac ttggcatcct tacgaagttt gtaggtatag
    14581 gtcaaaccgt cctgagaaac agtccaatcc tctgctaatg atggaataat attcccatat
    14641 tggtcatttt ctaataaccc gtctaccaaa tttgcaacaa tatcggatgt tgctgcgcgg
    14701 ttttctgcta gatagttcaa gctagatgga tcacttgaat aaacatagtt gtaggttttt
    14761 gacgccgtgc tagaatttcc acacgcgctc aataaaactc ctgtacccag gacaagacct
    14821 gccaaggtta gatatttgct cttagacttt ttcatttccg gaacctcctg ctttaaatat
    14881 ttgttttatt atacaactct tggtttgttt agtcaattgt tttttccaaa aaatcaaaaa
    14941 aaacctcatt ttctattttt gaaagataat tttagctatt tttacgctaa aaaaggaata
    15001 agtttcaagt tctcctaaaa gattgttcaa cttattcctt ttatttataa atgctacctt
    15061 acgaaagctt aaattttgct cgcaggcgat ctgcttgggc ttttcctatt accttatcta
    15121 acaaataggt acgcagactc ataaccatat aaagtccacc ccccatggca ccgacaagag
    15181 ctacataaaa gaagctccac aaacgtccac ttggttggaa gaaaaatcct aacagccact
    15241 ggatggttcc tattaacaga aacatgacta gggtcagcaa actgattaaa atggttcgct
    15301 tcaaaatcac cttgcgcttg acaccagtta ctttacaaat atcccgatac atcaagacgt
    15361 taggaatgat gagagcaatg gttgttgaaa tcaaaggacc ataactgtgg aagagggcga
    15421 tggtaggtag ttgcaagact agcttggcaa tagaaccata gataaaatag agaacggcct
    15481 tgcggttgcg gaacatggcc tgaagcattg gagacaagac catgtacaag cctaaaataa
    15541 tagactgcaa aactgcaaag acaaataagc ccagagccaa actatctggc ttaccataga
    15601 agactgtata tagaggttct cctaccataa ccactccaac cgttgctggt agcaagaaca
    15661 taaagagtag ggtgagactg tcctgaacca gacgagaagc tgctttcaag tcccccttga
    15721 catagttttc cgtcaaaagt ggcaaaccaa cactcccaat cgaaacccct acagaaatca
    15781 aaatcatcgt gattttatta ggattggctg agaaataaga aaacatgaca accaagtcct
    15841 cattgctgta gttggtaaac cagctcatac tattgataaa ggtcagctga tccaaaatct
    15901 ggaagagctg gatggcagac cctgtcagga taaaaggaat ggcttcctta atggtatcaa
    15961 ccaagagacg cttactgtta atcttatctc ctgtttcaaa gactcttttg agtgaacctt
    16021 cttgggcaag gaaataaatc aagactgcaa aactggctac cataccgaca aaggcagcaa
    16081 aggttgattg ggtaacggct gctagataat ctcctgaacc gagcttcata atgataaagg
    16141 ttgctaggag catccagata acacgaatga cctgctcagc aatttggctc atggcataag
    16201 gtttgaggtt attcatccct tggaaaaatc ctcggataac actcatagac gggaaaatca
    16261 agactcccca agccaagctt tgcatgattg ggatcaagtc tttgcccacg ccagacaagt
    16321 ctgctagcca aggagcaaag acatacaaga ctaaagcaaa aaccaggcct agtcctgtca
    16381 taaagcctaa gaagctccga atcagggcaa agctatgctc ttcttctcgc atggtattat
    16441 acttggcaac ttgcttggcc accgcaactg gaatccccgc tgttgaaacc aacaagaacc
    16501 aagcatagat attgtaaccc atggtaaaga gaccatttgc cttagctgca taagccccca
    16561 tccagatgta ccaagggata atgtaaacag ccccgagtag gcgactgata aagttactag
    16621 ccgttagcca agcagtcccc cgtaacatct gggcctgctg gtgattgttt tcgtgcgaca
    16681 tagattcctc attcaatttt gataactagg aaaaactcct tatcttccat tataaacttt
    16741 tccttgttac ttgtaaattt gcgactttcg gtttacaata gaaagtatga taaaaattga
    16801 aactgtatta gatattttaa agaaagatgg cctttttcgc gaaattattg accaaggtca
    16861 ttaccactac aactacagca aagttgtttt tgatagcatc agctacgata gccgaaaagt
    16921 aacagaagac actctttttt ttgcaaaagg cgctgccttt aaaaaagaat accttctttc
    16981 tgctataaca caaggcttag cttggtatgt agctgaaaag gactacgaag tcggtatccc
    17041 tgtcatcatt gtgaacgata taaagaaagc catgagtttg attgccatgg agttctatgg
    17101 taatccacag gaaaaactca aactccttgc ctttactggt actaagggta agacaacagc
    17161 agcctatttc gcctataaca tcttatctca agggcatcga cctgctatgt tgtcgaccat
    17221 gaacacaact cttgatggcg agactttctt taagtcagcg ttgacaaccc ctgagagtat
    17281 tgacctcttt gacatgatga atcaggctgt gcaaaatgac cgtacccacc tcatcatgga
    17341 agtctccagt caatcctatc tggtcagacg tgtctatggt ctaacctttg atgtgggagt
    17401 tttcctcaat atcagcccag accatatcgg cccgattgaa caccctagct ttgaagacta
    17461 tttctaccac aagcgtctct tgatggaaaa tagccgagca gtcatcatta acagtgacat
    17521 ggaccacttc tcagtcttga aagaacaggt tgaagatcaa gaccatgatt tctatggtag
    17581 ccaatttgat aaccaaatcg agaattccaa agcctttagc ttttcagcta cgggtaaact
    17641 cgctggagat tatgatatcc aactcattgg caacttcaac caagaaaatg cagttgctgc
    17701 tggacttgct tgtctccgtc tcggagcaag tcttgaggac atcaaaaaag gcatcgctgc
    17761 aacccgcgtt cctggtcgta tggaagtcct cactcagaaa aatggagcca aggtcttcat
    17821 cgactatgcc cacaatgggg atagtctgaa aaaactcatc aatgtggttg aaactcatca
    17881 aaccggaaag attgctctgg ttctgggatc aacaggaaac aagggagaaa gtcgtcgtaa
    17941 ggactttggc ctcctcctca atcaacaccc tgagattcaa gtctttctga ctgctgatga
    18001 ccctaactat gaagacccaa tggccattgc agatgaaatt agtagctaca tcaatcatcc
    18061 tgttgagaag attgcggatc gccaagaagc catcaaggcg gcaatggcta tcacaaatca
    18121 cgaattagat gcagttatta ttgcgggtaa gggagccgat tgttaccaaa tcatccaggg
    18181 caagaaagaa tcctacccag gagatacagc cgtcgcagaa aattatttat aagaatagaa
    18241 aaaatcaagg gaaaatgaaa cccttgattt tttcctattc tttagtaaaa acgtttttca
    18301 aaccttcaac ggcaccttct acagcacctt ttgcgtcttc tacaacgtct tttgccttag
    18361 caactgtttt ttcaacagct ccttcaagtt ctgtcttgct gtctccagta actttaccaa
    18421 aaccttcttt gacagcgcct gttgcttgtt ccaatttgtt ttcaagtgac atatgattgt
    18481 ctcctttagt ttattccgat atgtgttacc ggttacatat agtttatacc gaatactaag
    18541 gaaagtcaaa caatgtactc agaaacaaga aatcacgcta agtagaaagt taatctctcc
    18601 ttatcaaaat cgatagccaa ctcatacttt ccatcttcat tttgcacaat atagcctaag
    18661 acaaccaaac tgtccacaaa gatatcacgg catttctgca taagctggtc ttttttgaga
    18721 aatttcaaca aaaaagccgt catatacttg agtgcatact caggattaac atctcctaaa
    18781 atgtcataga gctcctgctg tttttctgtc aaaggatact gctgtttgac cttgtaaaaa
    18841 taattggaca gggtcatttt aatgcgcgca aagtccgtct tttcaactaa aatagctgca
    18901 ttgatttgat tgcgcaattc cgtctcaaaa ctctgctcca acaaggcttg atagaccgga
    18961 ctagcttctc tgacaaaaat ctcttgatcc agttcaagac tatcaagtga ttcaagcgta
    19021 ggaaaattga ggtaatagcg cttattttca cgtagaatca agcctgcctt tatatactct
    19081 tccatgagtt tatcaactgc aacatctgga aattgagcct taatttcccg tagaatcaca
    19141 tcctcatact ggtccagata gcggatcaat tctccaaaaa tggctgtctc gtcaaacgag
    19201 atggattaaa aatctgaatc atgaagattc ctttctttac attgtaacag aggcgatgct
    19261 gccaactgac taggattggt accagcttgg ttcagaggaa caggcagacc tagttctatg
    19321 taatactccc tccaaaaatc actaatctcc tgctccccat ctccaaattt cataggaata
    19381 gtttcaaaaa taggtaggat ttctgctata tactgggagc agtagaaacc aggtgcatct
    19441 ggatagaaag aagcattgta gggtgctcca agatgtttgc aagctctttc cttcaccgac
    19501 tggatatcca tttctggata aacatagagg tcgtacaaat gattggactc aaagaagtct
    19561 gccggttctt gacagacaac accagcctgt ccactagcat gataaatcat cccatccaaa
    19621 taaatggcaa catggctata gttacctgtg gaagtctgga tggcctgtcc catgtctgac
    19681 ccatctctca caaaaatcaa atcgccattt tctaacatac ttcactccta gaaaaaaagg
    19741 agccgaaact cctttcgata taaggcaaac tagactttcc gattttgagc tgacacccaa
    19801 aatcttacgc attaaagctt tcagttaatt gaggtaccac ttgtttctta cgtgaaacgg
    19861 caccagcaag gaaggcatga ttgttttcaa gtttgaagtt gaaagccgct tcgaccttgt
    19921 ccatattggc accaagagcc aagatttctg agtttgagtt gacgatatct gtaatcatca
    19981 agacaaagtc agagtagccg tttgattcgt tggcagcttg cattgcagct tcaatttctg
    20041 cttggcgttc caaaacttca gcgatgtcaa ctgtgttcac ttgggcaaca cggacattat
    20101 ttccgttgag ttcaaaagtc ttagcatcga tgtcaatcaa ttcttcagca gatttgctag
    20161 ccaagttggt accagctttc aacattgcca aaccatattc ttccaagttc acaccagcca
    20221 attcagccaa ttcaggagca atgattttat ctgttgggtg tgttgttggt gatttcaaaa
    20281 gaagggtatc tgaaatcaaa cctgaaagca tcaaacctgc aatctcttta ggcacagcta
    20341 caccatgttc tttgaacata cggtaaacga ttgaagacgc tgatccaact ggctccaaac
    20401 gcatgtaaag tgggcttgca gtttcaaagt tagccacacg gtggtggtct acaacaccgt
    20461 aaacttctac ttcagcgata tctgatacag attgttggaa ttcattgtgg tcagtcaaga
    20521 taacttgctc tgctccctct gctttggcag aagtgataac acgtggtgct tctacaccaa
    20581 aatagttcaa gacaaaggct gtttcttcat ttggagttcc aagggcaaca gcttccgtat
    20641 ccaaaccgta agcttctttt gcaaggtagg caaaagctac agatgatccg atggcatctg
    20701 agtctggatt ttggtgacca aatactagaa tcttggacat gataatacct cattttgttt
    20761 attttcttta ttgtagcatt tttttcgctt tttgacaaag gtaagaggag tcaaaggact
    20821 ccccacgatt cttcaaaata actgagtaaa tttctatctt gattttcaga taaaaattct
    20881 tccttctgtg gcctcttctt acgcttgaga agagcttcag ccgacatggc ttcttcctta
    20941 ctggcaaaac cttgagcata gataagtttg actggcaagc gtgctcgtgt atatttggct
    21001 cccttcccac tattgtggat agcgaggcgt cttctcacat cagtcgtata gcctatatag
    21061 taggatccat cacgacactc cagaacgtac atataagcct tatgatccat aataaatctc
    21121 ttcgatttcg ggcgtataag agccatcatc attgtggaca atcaaaggag gtaagacctt
    21181 aaagccactt gttgagccat ccttgatcgc ctcaatcaaa agcatattgg cttccttttc
    21241 tctttttgga taaacaaact gcaggcgctt aggggctaga ttatgtcgtt ttaacgtatc
    21301 caaaatatcc agaagtcgat caggacgatg aaccatggcc aaacgcccat tagacttgag
    21361 aatactctgg gcactacgac agatttcttc caaattagtc gtgatttcgt gtcgagccaa
    21421 gaggtaatgt tcactctcgt tcagattaga ataaggatcc accttgaaat agggtggatt
    21481 acacaaaatc atatccacct tactcccctg aatgtgagca ggcatatttt tcaaatcatc
    21541 gcagatgacc tgcatttgct cctctaatcc attcaaacgg acagagcgtt cagccatatc
    21601 cgccaaacgc tcctgaatct caacagacaa tatctgtgct tgagtacgag tgctagcaaa
    21661 aagccccact gctccattcc cagcacagaa atccacaatc agccccttct taggaaaacg
    21721 tggaaatcgt gataagagaa cactatccac cgaatagcta aaaacctctc tattttgaat
    21781 gattttgata tctgtcgaaa agagctggtt aatgcgctct cctgatttta ataattgttc
    21841 ttcttccatg gtcctattat agcaaattta tattaacatt acaaaaaata taaaactcta
    21901 aactacttct tcttttttaa atggtgcagg gcttctccag tccagattgg taacattcgt
    21961 cgaaagggag caaaaccgta gttaaagcgg tcgcttgaaa agcgtctccg tctaggaaac
    22021 tggtactttt cttcctccaa agtgcggata gaaagactgg ctttccccgt aaattcatct
    22081 aaatccacta cctgaacttg aacctcttca tcgactttca aggtttcatg aatattttca
    22141 ataaatcctg tccgaatttc tgaaatgtga atcagccccg tatcacccgt ctctaactca
    22201 acaaaggcac cgtagggctg aatccctgta atacgcccct ttagcttatc accgattttc
    22261 atcttagtcc tcgatttcaa tagtttcaat tacaacatct tcaactggct tgtccatagc
    22321 tcctgtctca acagcagcaa tggcatccaa gacagcgtaa gatgcttcat cagctaactg
    22381 accaaaaacc gtgtgacggc ggtctaggtg aggtgtccca ccttgattgg catagatttc
    22441 tgcaatcggt tctggccaac caccacgagt aatttctttc ttcgaataag gtaggtgttg
    22501 gttttgcacg ataaagaact ggctgccgtt ggtatttgga ccagcatttg ccatggaaag
    22561 agcaccacgg atattgtaaa gctcttctga gaattcatcc tcaaaagatt cgccgtagat
    22621 tgactcgcca cccataccag ttccagttgg gtctccacct tggatcataa agtccttgat
    22681 aatacggtgg aaaatgacac catcatagta gccatctttt gaaagagata caaagttagc
    22741 cactgtttta ggagcatgtt cagggaaaag cttgatacgt aagtctccgt gattggtctt
    22801 aatagtcgca agaggacctt ctactgtttc aatgtctact tgtggaaaat gcaattcttt
    22861 ttctaccata ccaaatactt ctaaggcagc aaaaatgcca tcttcttcta atgtttttgt
    22921 aatataatct gctttttctt tgattttatc atgagaaatt cccatggcaa cgctgattcc
    22981 agcataatca aagagttcca agtcattgag accatctcca aaaaccatga ccttctctgg
    23041 tttcaagcca aggtgttcca caaccttttc cacccccgtc gctttggagc ctgaaatcgg
    23101 cacaatatca gacgaatgtt gatgccaacg aaccatgcga agtttgtctg agagactgtc
    23161 aggcaagtgc aagtcatctc ccttatcttc aaaagtccac atctgataga tatcttcttt
    23221 ttcatggaaa tcgggatcta catctaagtc gggataaatt ggattgatag cttcactcat
    23281 catatcggtg cgagtcgaca acttggcatc atgactccca accaagccat actcaattcc
    23341 ttcttgctta gcccaagaga tatactcctc aacatctgac ttttcaatct gatgctgata
    23401 aatgacctga ccttttttat cttcgatata agccccattc aaagttacaa aaaagtcagg
    23461 cttgagatca cgaatctctg gaacaacacc aaaaatgcca cgtccagagg cgattcctgt
    23521 taaaattcct ttttcacgca actgtttaaa aacagtggga attgtagttg gaataaaccc
    23581 tgtctttgaa ttccgcaatg tatcatcaat atcaaaaaag acaatcttga tcttctttgc
    23641 cttgtatctt aatttcgcgt ccatctcact acctctttca atctaactct ttccattata
    23701 tcataaagta ggcaaatccc ctattttcaa aaagtttatc atttttattt taatttcttg
    23761 gatgagaaaa aagacatatt tatgaaaaag ctccatcgtg cttttaatgt gttctcttgt
    23821 tttcaaactc gtaaaaaggg agccactgag cctaactcgc tctctcattt caaagtttgt
    23881 gaaaaaagac ccattggggt cttaattcgc tttcttgttt tcaagctcat gaaaaagaga
    23941 cccaaccggg tcttttcttt aatcttcgtt tacgaaaggc atcaaagcca ttacgcgagc
    24001 gcgtttgata gctgttgtta ctttacgttg gtttttagct gaagttcctg ttacacgacg
    24061 aggaaggatt ttcccacgtt ctgaaacgaa acggctaaga agctcagtat ctttgtaatc
    24121 aacatattca attttgtttg ctgcgatgta atcaactttt ttacggcgtt tgaatccgcc
    24181 acgacgttgt tgagccatgt tttttctcct ttataagttt agttgtccat tagaatggta
    24241 aatcatcatc tgaaatatcc aatgggtttg ttgctccaaa tggattttca ttacgtgaaa
    24301 agtctggtac tgaatttgta ggtgctgaat agtttgcagt tggtgcagag taagctccac
    24361 ctgtgtgacc ctcacgcaca ctacggcttt ccaacatttg gaaattctca gccacgacct
    24421 ctgtcacgta gacacgttgt ccttgctggt tatcgtaact acgagtctgg atacgacctg
    24481 tcaccccgat aagtgagcct tttttagccc agttagcaag attttcagcc tgttggcgcc
    24541 acataacgac attgataaaa tcagcctcac gttcaccatt ttgactctta aatgtacggt
    24601 ttactgcaag agtaaaagtc gcaactgcta catttgatgg ggtataacgc aactcagcgt
    24661 cacgtgtcat acgccctaca agtacaacat tgttaatcat agtttacctt cttacgcgtc
    24721 aattttgacg atcatgtgac gaagaatgtc agcgttgatt tttgaaagac ggtcaaactc
    24781 tttaagagct gcatcgtcat ttgcttcaac gttaacgatg tggtaaagtc cttcacggaa
    24841 atcttggatt tcgtatgcaa gacgacgttt ttcccaagtt tttgattcaa caacagttgc
    24901 accgttgtca gtcaaaatag agtcaaaacg tgctaccaaa gcgtttttag cttcttcttc
    24961 aatgtttgga cgaatgatat aaagaatttc gtatttagcc attgatatgt tcctcctttt
    25021 ggtctaatga ccccaagact ttgcaagggg taagtgaggt tcgctcacaa taaactatta
    25081 tactagaaaa aattttttta cgcaagtaaa aacactagaa ttcgaaaaaa cgccacatgg
    25141 gcgttttcct gttcttatgg tttgatacgg tgcaacatac gtgggaatgg aatagcttca
    25201 cggatatgtt ttgttcctgc tgcgaaggtt accatacgtt cgataccgat accaaatcct
    25261 ccgtgtggaa ctgtaccgta tttacgaagg tcaaggtaga attcatattc tgtacgatcc
    25321 atgccaagtt catccatctt agcgacaagg gcatcgtaat cttcctcacg catagaccca
    25381 ccgataattt ctccatagcc ttctggagca agcaagtctg cacaaagcac gcgctctgga
    25441 tttccaggaa ctggtttcat gtagaaggcc ttgatggctg ctggatagtt catgacaaat
    25501 gttggcacac caaagtggtt tgaaatccaa gtttcgtgtg gtgacccaaa gtcatcacca
    25561 tgctcaagat gctcgtagtc agcatcttca tcattttcat gctcttgcaa gaggtcaatg
    25621 gcttgatcgt aagtgatacg tttgaatggc tctgcaatgt agcgtttcaa gagttctgta
    25681 tcacgttcca aggtttccaa ggcttgaggc gcgcggtcaa gaacaccttg tagaagagct
    25741 ttcacataag cttcttgcaa gtcaagcgac tcatcatgtg tcaagtatga gtactcagca
    25801 tccatcatcc agaactcagt caagtgacgg cgtgtttttg atttttcagc acggaaaact
    25861 ggaccaaagt caaagacacg accaagagcc atagcccctg cttctaggta aagctgacct
    25921 gattggctca agtaggctgg cgttccgaag tagtcagttt caaagagttc tgtagaatct
    25981 tctgccgcat ttcctgaaag aattgggctg tcaaacttca taaaaccgtt cttgtcaaag
    26041 aactcataag ttgcatagat aatagcgtta cggatttgca acacagctac ttgcttacga
    26101 gagcgtagcc acaagtgacg gttatccatc aaaaagtctg ttccgtgttc ttttggtgtg
    26161 attgggtagt cttgagattc accgatcact tcgatgtctg tgatgtccaa ctcatagcca
    26221 aatttagaac gttcgtcctc tttgacaata cctgtcacat aaacagacgt ttcttggctc
    26281 aagcgtttga taacatcaaa cttctcaagt cccacttctt caccaaattt ttcgacaaag
    26341 tttggtttaa aagccacacc ttgaaagaag gctgttccat cacgcaattg taagaaagcg
    26401 atttttcctt ttcctgattt gttggcaacc caagcgccaa tcgtcacttc ctgaccaaca
    26461 tagtctttta cgtcaataat cgttacacgt tttgtcatta tttttccttt tcttttttat
    26521 tctttatggc aaaccacctc tatattgttc ccatccaggt caatcataaa agcagcatag
    26581 taaatcggat gctcacttcg ataaccagga accccattgt ctcgcccacc tgcctctatg
    26641 ccagcctcat aacaagcctg aacttcttcc ttattttctg ctaaaaaagc aaaatgaaca
    26701 ggatcttgtg ttccctgagt cagccaaaaa tcaccaccag gatgagggct gttcggggat
    26761 agaaaactaa ttagagaact agtcttaaaa gccattttat agtccaaagg agcgagaaaa
    26821 ctcctataaa atccttctga aatttgtaaa tcctttacct taatctcaaa atgatcaatc
    26881 attctcacta cccataaatg ctttcaagcg ttcgactgct tctttaagcg tgtctaggtc
    26941 tgtcgcatag ctgaggcgga cattttctgg tgctccaaat ccagctcctg ttaccaaggc
    27001 cacttcggct tcttctaaga tagcagttgt aaagtctatc acatccgtgt agcctttcat
    27061 ctccatggcc tttttgacat ttgggaagag atagaaggcc ccttgcggtt tgaccacttc
    27121 aaatcctggc acctctgcaa ggaggggata gatagtatta agacgttcct caaaggcctg
    27181 acgcatgctt tctacagtat cttgctcacc tgatagagcc tcaactgctg catattgggc
    27241 tactgctgat ggattcgaag ttgtttgacc tgcaatcttg gacatggcag cgataatgtc
    27301 tgcttctcca acggcataac caatccgcca accagtcatg gcataggttt tagacacacc
    27361 attgatgacc actgtttgct tgcgaatcgc ttccgatagg ctagaaatcg gtgtgaactc
    27421 atgaccatta taaaccaagc ggccatagat atcgtctgct aggatgagaa tatcattttc
    27481 tacagcccag tttccaattg ccaagagttc ctcacgggtg taaatcatac ctgtgggatt
    27541 agatggcgaa ttcagcacca aaaccttggt cttgtcagtc cgagctgctt ctaactgctc
    27601 cacggtcacc ttaaagtgat tgtcttcctt agcagaaaca aagacgggaa cgccttctgc
    27661 catcttgacc tgatctccat agctaaccca gtatggggtt gggatgatga cttcatcacc
    27721 tggattgacc acagccataa agaaggtata gagagaatat ttggctcccg cagcgactgt
    27781 cacttgattt gacgctacag aatagccgta aaagcgctca aagtagctat tgaccgccgc
    27841 cttaagctct ggcagacctg aggttactgt ataaaaagaa gcacgcccat ctcgaatcga
    27901 tgcaatggcg gcatcttgga tatttttggg agtagtgaaa tctggctcac ccaaggttag
    27961 agacaaaata tctctaccct cagccttcag tgctttggca cgggctccag cagccaaagt
    28021 cacactttct tccatttcta aaacacggtt ggataatttc ataggccctc cttgttgacc
    28081 aatgctcctg tttcaaaatc tactagataa aaatcagatc ctgacttaac ttcccagatt
    28141 ggcttatctt gataacggcc aaaggttatc ttgtcaatct cgccagctcc cttttcctta
    28201 gaaaccgttt ctgctttttc ttgtgaaaca ccctgattta gctgataaac gtaaatctta
    28261 tggtcatctt taccaatcag gacagcaagc gcttcttgct gtttattacg accaagaacg
    28321 ctgtaataag attccaagcc attgtataaa tcaacctgaa cagcctgctc taatcctgca
    28381 tactgctgag ctaatttttc tccttcactt ttagctgttt gatagggttt catgctaaga
    28441 gaaaccatat acagaaagga accactgata accacaaaca aaatcgtcat ccctagacca
    28501 tactgccaca gtagattatt ttttgctttg ttttgtcttt ttttcactcg tctattttac
    28561 catctattaa gctttattac aagtgaatat aagaatactc ttcgaaaatc tcttcaaacc
    28621 acgtcagctt tatctgcaac ctcaaagctg tgctttgagc aaccaattct atttctccct
    28681 tcaaacaaaa ccgattttga aagtgaaaca gttcttactt tttcagtcac aaatgactag
    28741 agtttccgaa attttacaat tttcaaataa actctgagca aatttcttgc aagttctttt
    28801 gttttgttgt aatataattt ataacaacga gagaattctc gaaattttaa gaaaaaggag
    28861 acacatcatg tctaaaaaag tattatttat cgtcggatca ctacgtcaag gttctttcaa
    28921 ccaccaaatg gcgctcgaag ctgagaaagc acttgctggt aaagcggaag ttagctacct
    28981 tgattattca gcccttcctc tctttagcca agatttggaa gttccaacac atccagctgt
    29041 agctgctgct cgtgaagcag ttctcgttgc ggatgctatc tggattttct ctccagtcta
    29101 caacttctct atccctggta cagtgaaaaa cttgcttgac tggctatctc gtgcccttga
    29161 cttgtctgat acacgtggcg tttctgccct tcaagacaag tttgtcacag tatcatctgt
    29221 agccaatgca gggcacgatc aacttttcgc tatctacaaa gacctcttgc catttatccg
    29281 tacacaaggc gttggtgatt tcactgctgc acgtgttaat gactctgcct gggcagacgg
    29341 aaaattggtt cttgaagaaa cagtcctaaa ctcacttgaa aaacaagctc aagacttggt
    29401 cgaagctatc aagtaactaa cactcaataa aaatcaaaaa gcaaactagg aagctagccg
    29461 caagctactc aaagcactgc tttgaggttg tagatagaac tgacgaagtc agtaacatat
    29521 atacggtaag gcgacactga catggcttga agagattttc gaagagtata acataataaa
    29581 agcgaacaag actacttttc tgacgctcag aagactagct tgttcgcttt tttctcattt
    29641 ataaactaaa atagctgatg atacatattg ccaaatacaa aatatctgat ttctatactt
    29701 cctttacaaa aaccaatccc tgtggttgtg acaaatcttc tcggtaaaca aatccagcaa
    29761 agttcaagcg acgtgcttcc cccacagttt gtacttgatt ttctattaaa gctgttagaa
    29821 aggccccgcg cgctttcttg gagatagttg agtgaatctt cagctgaccg cctctatcct
    29881 ccatgaattt gaaggtcacc atctttgctc tgatttcctt agaaaataca gtctcaaact
    29941 ctgatgacaa gagagagaaa atcacttctt ccttcttcag agcttcatca tagactgcct
    30001 tccaatggct cttcaaagtc ttaccagcaa cttttaattt catcaaaaaa tccaaacggt
    30061 gaggagccat gggtgacaag gctggaacaa caccgtacaa agccgaggta atgaagacat
    30121 gattttcaag ataatcttgt tccgcctcgg tcagcttatc tctcttaatg ttgcggtaca
    30181 taagcccatc aaaaagtttc aaggctggat agtgttgagt agtttgcctt ttcaaagctt
    30241 ggatattttg aaattcttcc gccgctttct cagctgatac cttgtagaaa ctctccaatt
    30301 gattggcaga atagagagcc aaggcatcaa gcacggcctg actttctggt tttaaagaaa
    30361 ttgcctcgat acttggcaag tctgtgttca tttcttttgc tgttgggatt aaaattttca
    30421 tattgatagt gtagcatatt ttttagccac aaccaagaaa ttcatttgaa aaacctcggt
    30481 ttgaccgagg tttttctatt tatttgggcc atcctaacca gacagccacg agaacaaggg
    30541 ctgtgccagc taaacttgtt aagatagata agaatttatc ttttttctcc ttgaactgag
    30601 aaaaacctat cttcaaagcg agcagtccga gtagcattga agcaaccatc aaataaaaac
    30661 ccatttcttt gtcctccatt aggcttaatt taccttatta taacatatac tttctgaaaa
    30721 aaacctattt ttaccgtagc ttaaaggtct tgagataatt gtcttttcct acttgacctt
    30781 cgaaggtttt accattttca aggtaaggaa ggtcatcgga tactgaagcc ttgaccttgt
    30841 atatcttgcc atcaacttta aagaagtaga cagtgtctcc cttgataaca gctgatttga
    30901 ggtctgctac tactccattg atgctttctg tcgttgcatt gtcaatttca aggtcgtttt
    30961 tattggcata cttgctgagc atctcttcca ctgtagtagc aacgataaca ttttggtact
    31021 cgactgcgtc taccagggcg tactctttga ccaagccagc attgtccttc aagcccatga
    31081 tgtagagagg cttgtcattg aggttgatga ggattgggaa ggttgctttg taggatttct
    31141 cctgaacagc accttctgct gattcacggg ctgattcttc tgtcgcagaa gccaagctat
    31201 acttagtgat ttctcctgtt cgcatatttt caaggatgaa accaagatta ctctcatccg
    31261 cattagccga cgtcacacct gtgtagagat agatgtcatt accgatagac aagtaattat
    31321 agcccttggt agtctgggtc acgtttttct tggaaatcat ggcattcaag aaaccgtcct
    31381 tgtacttgcc gttgtagttg atttgctcaa tggtttcctc tgctggatag atcctgtcca
    31441 cccattctgg aacatctgat aagctgtatt ccttggtttc tccatttgta gcatccaaga
    31501 taatgactga agcaggacga ggaacagcaa gtccaaattg cttttggtaa accgttgcta
    31561 catagaaagg attgccctca tcgtccacct caaaagatgg agttttaaag attttggtcg
    31621 ggtacttcaa gcgcaggtga cgtttgacat cacggttaaa atactccgag tctgaatact
    31681 tgattggtgt cttcaagtcc accaaatccg catttccagt taccatgtcc accttgatat
    31741 actcaccgat tcccttggct tgattgttaa accatttgat agggtctgca tattctagtg
    31801 gtgtgacccg ataaggtttc ccatcaattg tcaattgggt ataggtatct gccgctacgt
    31861 attgcgacac cttatcggtt agggaaccca agtagcggtc tccaattttt tcagcagtac
    31921 ttctatctag gataggaacc ttactggtgt cactcctagg aaattcagta aagtcttttt
    31981 ccgtaaccgt gactacattg gcataatttt tagcctgaaa catgctggaa gttaccaagg
    32041 aaaccaaagc tgccaagaga agaattcctc caatcgaagc taacagaatt ttccctaacc
    32101 gattgatttt gaaaccctca agatttaagg cagcttccgc cttaccgtgg cgcacatgaa
    32161 ccgttttgac aaggtttatc cccttgccaa agccaaataa gattgccaca actaacaaat
    32221 gcccacagag gaagaagaga aattcccagc tggtcaggtt aaggggcggt aaaaagatat
    32281 accaggtcgt tgcgataaaa ataagttcaa agactattcg tttcatttgc tttcctccta
    32341 aatgattcgt ccttccaagt gatccagctc atgctggcaa atctgagctg ggaagcctgt
    32401 caaggtaatg gtctgttcct gccacttgct gtcacgatag gcaaccctta tggtttcata
    32461 acgcttagtt gatctcacgc ctaccaagga caaacagcct tcttctgcct cataggatcc
    32521 ttcaaaggac agaagcactg ggttaaacat gaccacggga accaagccaa gattaaagat
    32581 aatcacgcgc ttctgcaccc caatcatatt ggcagctaga ccaacacagg tatcatgatt
    32641 tgctaagagt gtatcctgca aatctctggc aagataaagg tcctcctgac ttgccggctg
    32701 agacacttga gataaaaata agatatcctt cacaattttc ttttccactt cctcacactc
    32761 ctcttgcttt ttactatatt atagcaatta tagcacaaaa gccgataact gcaaaccctt
    32821 tccctcaact gtagcaaaaa gccatccgaa gatgactttt ttgctattta att
//
LOCUS       DAVLVP010000016        30742 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.016, whole genome shotgun sequence.
ACCESSION   DAVLVP010000016 DAVLVP010000000
VERSION     DAVLVP010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 30742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30742
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.016"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..785)
                     /gene="yghU"
                     /locus_tag="VYN69_000695"
     CDS             complement(<1..785)
                     /gene="yghU"
                     /locus_tag="VYN69_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001277405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HEV1533007.1"
                     /translation="MSAYQLPTVWQDEASNQGAFTGLNRPTAGARFEQNLPKGEQAFQ
                     LYSLGTPNGVKVTILLEELLEAGFKEAAYDLYKIAIMDGDQFGSDFVKLNPNSKIPAL
                     LDQSGTENVRVFESAHILLYLAEKFGAFLPSNPVEKVEVLNWLFWQAGAAPFLGGGFG
                     HFFNYAPEKLEYPINRFTMEVKRQLDLLDKELAQKPYIAGNDYTIADIAIWSWYGQLV
                     QGNLYQGSAKFLDASSYQNLVKWAEKIVNRPAVKRGLEVTYTE"
     gene            complement(798..3494)
                     /locus_tag="VYN69_000696"
     CDS             complement(798..3494)
                     /locus_tag="VYN69_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEV1533008.1"
                     /translation="MSKEQKRQAFYTQSPEEVLQAVDATEQGLSSSEAEKRLAEFGHN
                     ELEEGEKRSILVKFIEQFKDLMIIILVAAAILSVVTSGGEDIADAIIILAVVIINAAF
                     GVYQEGKAEEAIEALKSMSSPVARVLRDGHMAEIDSKELVPGDIVALEAGDVVPADLR
                     LIEANSLKIEEAALTGESVPVEKDLSVELATDAGIGDRVNMAFQNSNVTYGRGMGVVV
                     NTGMYTEVGHIAGMLQDADETDTPLKQNLNNLSKVLTYAILVIALVTFVVGVFIQGKN
                     PLGELLTSVALAVAAIPEGLPAIVTIVLSLGTQVLAKRHSIVRKLPAVETLGSTEIIA
                     SDKTGTLTMNKMTVEKVFYDAVLHDSADDIELGLEMPLLRSVVLANDTKIDVEGNLIG
                     DPTETAFIQYALDKGYDVKGFLEKYPRVAELPFDSDRKLMSTVHPLPDGRFLVAVKGA
                     PDQLLKRCLLRDKAGDIAPIDEKVTNLIHTNNSEMAHQALRVLAGAYKIIDSIPENLT
                     SEELENDLIFTGLIGMIDPERPEAAEAVRVAKEAGIRPIMITGDHQDTAEAIAKRLGI
                     IDANDTEGHVLTGAELNELSDEEFEKVVGQYSVYARVSPEHKVRIVKAWQKQGKVVAM
                     TGDGVNDAPALKTADIGIGMGITGTEVSKGASDMILADDNFATIIVAVEEGRKVFSNI
                     QKTIQYLLSANTAEVLTIFLSTLFGWDVLQPVHLLWINLVTDTFPAIALGVEPAEPGV
                     MNHKPRGRKASFFSGGVLSSIIYQGVLQAALVMSVYGLAIAYPVHVGDNHAIHADALT
                     MAFATLGLIQLFHAYNVKSVYQSILTVGPFKSKTFNWSILVSFILLMATIVVEPLEGI
                     FHVTKLDLSQWGIVMAGSFSMIIIVEIVKFIQRKLGFDKNAI"
     gene            3798..4982
                     /gene="mntE"
                     /locus_tag="VYN69_000697"
     CDS             3798..4982
                     /gene="mntE"
                     /locus_tag="VYN69_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family manganese efflux transporter MntE"
                     /protein_id="HEV1533009.1"
                     /translation="MKQSISNLKLAERGAIISISTYLILSAAKLAAGHLLHSSSLVAD
                     GFNNVSDIIGNVALLIGIRMARQPADRDHRFGHWKIEDLASLITSIIMFYVGFDVLRD
                     TIQKILSREESVIDPLGATLGIISAAIMFVVYLYNTRLSKKSNSKALKAAAKDNLSDA
                     VTSLGTAIAILASSFNYPIVDKLVAIIITFFILKTAYDIFIESSFSLSDGFDDRLLED
                     YQKAIMEIPKISKVKSQRGRTYGSNIYLDITLEMNPDLSVFESHEIADQVESMLEERF
                     GVFDTDVHIEPAPIPEDEILDNVYKKLLMREQLIDQGNQLEELLTDDFVYIRQDGEQM
                     DKEAYKTKKELNSAIKDIQITSISQKTKLICYELDGIIHTSIWRRHETWQNIFHQETK
                     KE"
     gene            complement(5125..6996)
                     /locus_tag="VYN69_000698"
     CDS             complement(5125..6996)
                     /locus_tag="VYN69_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001279112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1533010.1"
                     /translation="MSDFIVEKLSKSVGDKTVFRDISFIIHDLDRIGLIGVNGTGKTT
                     LLDVLSGVSGFDGDVSPFSAKNDYQIGYLTQDPDFDDRKTVLDTVLSSELKEIQLIRE
                     YELIMLDYSEDKQARLERVMAEMDSLQAWEIESQVKTVLSKLGIQDLSTPVGELSGGL
                     RRRVQLAQVLLGNHDLLLLDEPTNHLDIAIIEWLTLFVKNSKKTVLFITHDRYFLDAL
                     STRIFELDRAGLTEYQGNYQDYVRLKAEQDERDAALLHKKEQLYKQELAWMRRQPQAR
                     ATKQQARINRFHDLKKKVSGSSAETDLTMNFETSRIGKKVIEFQAVSFAYENKPILQD
                     FNLLVQAKDRIGIVGDNGVGKSTLLNLIAGSLEPTKGQVVIGETVRIAYFSQQIEGLD
                     ESKRVINYLQEVAEEVKTSGGSTTSIAELLEQFLFPRSTHGTLIEKLSGGEKKRLYLL
                     KLLLEKPNVLLLDEPTNDLDIATLTVLENFLQGFAGPVLTVSHDRYFLDKVATKILAF
                     EDGKIRPFFGHYTDYLDEKAFETDMANQVQKVEKEKVVKVREDKKRMTYQEKQEWASI
                     EGDIETLEKRIAAIEEEMQANGSDFGKLATLQKELDEKNEALLEKYERYEYLSEFDS"
     gene            complement(6993..8177)
                     /locus_tag="VYN69_000699"
     CDS             complement(6993..8177)
                     /locus_tag="VYN69_000699"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEV1533011.1"
                     /translation="MPSEFQKALPVLEKIKEAGFEAYFVGGSVRDALLHSPIHDVDIA
                     TSSYPEETKQIFPRTADIGIEHGTVLVLDGDEEYEVTTFRTEDVYVDYRRPSAVSFVR
                     SLEEDLKRRDFTVNAFALDETGEIVDLFHGLEDLEKQVLRAVGVASERFNEDALRIMR
                     GFRFQASLGFALEPETFKAMKTLTPLLEKISVERTFVEFDKLLLAPFWRRGLASMIES
                     QAYDYLPDMASSQDKLNRLFDLETDFTFESSEQAWAALLWALEIENAQSFLKSWKTSR
                     QFAKQVQDLLIILALRENGELSKRDCYRFDIDLLLQAENLRQAQGKEVNPQAITEKYQ
                     SLTIHDKKEIQINGGILIKEYGYQPGPDLGEILTEIEFAIVDGELENNREAIHAYLRE
                     KK"
     gene            complement(8189..8956)
                     /locus_tag="VYN69_000700"
     CDS             complement(8189..8956)
                     /locus_tag="VYN69_000700"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEV1533012.1"
                     /translation="MSIRVIIAGFKGKMGQAACQMVLPDSDLDLVAVLDPFESESEWQ
                     GIPVFKDKADLAGFEADVWVDFTTPAVAYENTRFALENGFAPVVGTTGFTSEEIAELK
                     EFSRAQDLGGLIAPNFALGAVLLMQFATQAAKYFPNVEIIELHHDKKKDAPSGTAIKT
                     AELMAEVRESIQQGAADEEELIAGARGADFDGMRIHSVRLPGLVAHQEVIFGNQGEGL
                     TLRHDSYDRISFMTGVNLGIKEVVKRHELVYGLEHLL"
     gene            complement(9233..10081)
                     /locus_tag="VYN69_000701"
     CDS             complement(9233..10081)
                     /locus_tag="VYN69_000701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1533013.1"
                     /translation="MKLAVFTDSSAYLSEETLQREDLFVLDIPVNIDGEEYVEGINLS
                     AEEFYQKMAQASELPKTSQPSIAKLDEILTSLKEQGYTHALGLFLSSGISGFYQNIQY
                     MVDDYEGLTIAFPDTLITSAPLGIMVESVFNWRDQGDDFASIQDKLAIQISRTSAFIM
                     VDDLDHLVKGGRLSNGAAILGNLLSIKPILYFNDQGVIEVYEKVRTEKKATKRLIEII
                     KETTASGQYRVIVIHGNAPEKAEELRQHLLDFGLGSDVSLATFGSVIGTHLGSGSIAL
                     GYIPVI"
     gene            complement(10083..10457)
                     /locus_tag="VYN69_000702"
     CDS             complement(10083..10457)
                     /locus_tag="VYN69_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001050081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEV1533014.1"
                     /translation="MNLKREQEFVSQYHFDARNFEWENENGAPETKVDVNFQLLQHDQ
                     ENQVTSLIVILSFMIVFDKFVISGTISQVNHIDGRIVNEPSELNQEEVETLARPCLNM
                     LNRLTYEVTEIALDLPGINLEF"
     gene            complement(10531..11883)
                     /locus_tag="VYN69_000703"
     CDS             complement(10531..11883)
                     /locus_tag="VYN69_000703"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEV1533015.1"
                     /translation="MGKYFGTDGVRGEANLELTPELAFKLGRFGGYVLSQHETEAPKV
                     FVGRDTRISGEMLESALVAGLLSVGIHVYKLGVLATPAVAYLVETEGASAGVMISASH
                     NPALDNGIKFFGGDGFKLDDEKEAEIEALLDAEEDTLPRPSAEGLGILVDYPEGLRKY
                     EGYLVSTGTPLDGMKVALDTANGAASTSARQIFADLGAQLTVIGETPDGLNINLNVGS
                     THPEALQEVVKESGSAIGLAFDGDSDRLIAVDENGDIVDGDKIMYIIGKYLSEKGQLA
                     QNTIVTTVMSNLGFHKALNREGINKAVTAVGDRYVVEEMRKSGYNLGGEQSGHVILMD
                     YNTTGDGQLSAVQLTKIMKETGKSLSELAAEVTIYPQKLVNIRVENVMKEKAMEVPAI
                     KAIIEKMEEEMAGNGRILVRPSGTEPLLRVMAEAPTTEEVDYYVDTITDVVRAEIGID
                     "
     gene            complement(11907..12686)
                     /locus_tag="VYN69_000704"
     CDS             complement(11907..12686)
                     /locus_tag="VYN69_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HEV1533016.1"
                     /translation="MKKNSLYIISSLFFACVLFVYATATNFQNSTSARQVKTETYTNT
                     VTNVPIDIRYNSDKYFISGFASEVSVVLTGANRLSLASEMQESTRKFKVTADLTDAGV
                     GTIEVPLSIEDLPNGLTAVATPQKITVKIGKKAQKDKVKIVPEIDPSQIDSRVQIENV
                     MVSDKEVSITSDQETLDRIDKIIAVLPTSERITGNYSGSVPLQAIDRNGVVLPAVITP
                     FDTIMKVTTKPVAPSSSTSNSSTSSSSETSSSTKATSSKTN"
     gene            complement(12673..13530)
                     /locus_tag="VYN69_000705"
     CDS             complement(12673..13530)
                     /locus_tag="VYN69_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001867651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEV1533017.1"
                     /translation="MNFQQLSNLQYWTSLFASPWTIAINLIDILIVAYILYHFTKAIA
                     GTKIMILVRGVLVFILAQILANMIGLTTISWLINQIITYGVIAAVVIFSPEIRTGLER
                     LGRATDFFSNAPISAEEQMIRAFVKSVEYMSPRKIGALVAIQRVRTLQEYISTGIPLD
                     AKISAELLINIFIPNTPLHDGAVIIKEERIAVTSAYLPLTKNTGISKEFGTRHRAAIG
                     LSEVSDALTFVVSEETGGISITYNGRFKHNLTLDEFETELREILLPKEEVGLSFKERL
                     LGGWKHEKK"
     gene            13666..14631
                     /locus_tag="VYN69_000706"
     CDS             13666..14631
                     /locus_tag="VYN69_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEV1533018.1"
                     /translation="MSQLYDITIVGGGPVGLFAAFYAHLRQAKVQIIDSLPQLGGQPA
                     ILYPEKEILDVPGFPNLTGEELTNRLIEQLNGFDTPIHLNETVLEIDKQEEFAITTSK
                     GSHLTKTVIIAMGGGAFKPRPLELEGVEGYENIHYHVSNIQQYAGKKVTILGGGDSAV
                     DWALAFEKIAPTTLVHRRDNFRALEHSVQALQESSVTIKTPFAPSQLLGNGKTLDKLE
                     ITKVKSDETETIDLDHLFVNYGFKSSVGNLKNWGLDLNRHKIIVNSKQESSQAGIYAI
                     GDCCYYDGKIDLIATGLGEAPTAVNNAINYIDPEQKVQPKHSTSL"
     gene            complement(14665..15576)
                     /gene="whiA"
                     /locus_tag="VYN69_000707"
     CDS             complement(14665..15576)
                     /gene="whiA"
                     /locus_tag="VYN69_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEV1533019.1"
                     /translation="MSFTVAVKEEILGQHHLSWHELSAIIKMSGSIGLSTSGLTLSVV
                     TENAKLARHLYESFLHFYEIKSEIRHHQRSNLRKNRVYTVFTDEKVQDLLSDLHLADS
                     FFGLETGIDEAILSDEEAGRAYLCGAFLANGSIRDPESGKYQLEISSVYLDHAQGIAS
                     LLQQFLLDAKVLERKKGAVTYLQRAEDIMDFLIVIGAMQARDDFERVKILRETRNDLN
                     RANNAETANIARTVSASMKTINNISKIKDIMGLENLPVDLQEVAQLRIQHPDYSIQQL
                     ADSLSTPLTKSGVNHRLRKINKIADEL"
     gene            complement(15573..16550)
                     /locus_tag="VYN69_000708"
     CDS             complement(15573..16550)
                     /locus_tag="VYN69_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEV1533020.1"
                     /translation="MRKPKITVIGGGTGIPVILKSLREKDVEIAAIVTVADDGGSSGE
                     LRKNMQQLTPPGDLRNVLVAMSDMPKFYEKVFQYRFSEDAGAFAGHPLGNLIIAGLSE
                     MQGSTYNAMQLLSKFFHTTGKIYPSSDHPLTLHAVFQDGTEVAGESHIVDHRGIIDNV
                     YVTNALNDDTPLASRRVVQTILESDMIVLGPGSLFTSILPNIVIKEIGRALLETKAEI
                     AYVCNIMTQRGETEHFTDSDHVEVLHRHLGRPFIDTVLVNIEKVPQEYMNSNRFDEYL
                     VQVEHDFVGLCKQVSRVISSNFLRLENGGAFHDGDLIVDELMRIIQVKK"
     gene            complement(16547..17437)
                     /gene="rapZ"
                     /locus_tag="VYN69_000709"
     CDS             complement(16547..17437)
                     /gene="rapZ"
                     /locus_tag="VYN69_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEV1533021.1"
                     /translation="MTKKQLHLVIVTGMSGAGKTVAIQSFEDLGYFTIDNMPPALLPK
                     FLQLVEIKEDNPKLALVVDMRSRSFFSEIQAVLDELENQDGLDFKILFLDAADKELVA
                     RYKETRRSHPLAADGRILDGIKLERELLAPLKNMSQNVVDTTELTPRELRKTLAEQFS
                     DQEQAQSFRIEVMSFGFKYGIPIDADLVFDVRFLPNPYYLPELRNQTGVDEPVYDYVM
                     NHPESEDFYQHLLALIEPILPSYQKEGKSVLTIAMGCTGGQHRSVAFAKRLVQDLSKN
                     WSVNEGHRDKDRRKETVNRS"
     gene            complement(17489..17869)
                     /locus_tag="VYN69_000710"
     CDS             complement(17489..17869)
                     /locus_tag="VYN69_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HEV1533022.1"
                     /translation="MAKTIHTDKAPKAIGPYVQGKIVGNLLFASGQVPLSPETGEIVG
                     ENIQEQTEQVLKNIGAILAEAGTDFDHVVKTTCFLSDMNDFVPFNEVYQTAFKEEFPA
                     RSAVEVARLPRDVKVEIEVIAEIG"
     gene            complement(17880..18467)
                     /locus_tag="VYN69_000711"
     CDS             complement(17880..18467)
                     /locus_tag="VYN69_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002915299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEV1533023.1"
                     /translation="MELNTHNAEILLSAANKSHYPQDELPEIALAGRSNVGKSSFINT
                     MLNRKNLARTSGKPGKTQLLNFFNIDDKMRFVDVPGYGYARVSKKEREKWGCMIEEYL
                     TTRENLRAVVSLVDLRHDPSADDVQMYEFLKYYEIPVIIVATKADKIPRGKWNKHESA
                     IKKKLNFDPSDDFILFSSVSKAGMDEAWDAILEKL"
     gene            complement(18476..19708)
                     /gene="clpX"
                     /locus_tag="VYN69_000712"
     CDS             complement(18476..19708)
                     /gene="clpX"
                     /locus_tag="VYN69_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002874765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEV1533024.1"
                     /translation="MSTNRKNDMMVYCSFCGKNQEEVQKIIAGNNAFICNECVELAQE
                     IIREELVEEVLADLSEVPKPIELLHILNHYVIGQDRAKRALAVAVYNHYKRINFHDTR
                     EESEDVDLQKSNILMIGPTGSGKTFLAQTLAKSLNVPFAIADATALTEAGYVGEDVEN
                     ILLKLLQVADFNIERAERGIIYVDEIDKIAKKSENVSITRDVSGEGVQQALLKIIEGT
                     VASVPPQGGRKHPQQEMIQVDTKNILFIVGGAFDGIEEIVKQRLGEKVIGFGQNNKAI
                     DENSSYMQEIIAEDIQKFGIIPELIGRLPVFAALEQLTVDDLVRILKEPRNALVKQYQ
                     TLLSYDDVELEFDDEALQEIANKAIERKTGARGLRSIIEETMLDVMFEVPSQENVKLV
                     RITKETVDGTDKPILETA"
     gene            complement(19740..19910)
                     /locus_tag="VYN69_000713"
     CDS             complement(19740..19910)
                     /locus_tag="VYN69_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000442275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533025.1"
                     /translation="MERSLFGLFTAFLCFICFLAGAQAFRKKRYGLSILLWLNAFTNL
                     VNSIHAFYMTLF"
     gene            complement(19910..20416)
                     /locus_tag="VYN69_000714"
     CDS             complement(19910..20416)
                     /locus_tag="VYN69_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEV1533026.1"
                     /translation="MTKKIVAIWAQDEEGVIGKENRLPWHLPAELQHFKETTLNHAIL
                     MGRVTFDGMGRRLLPKRETLILTRNPEEKIDGVATFQDVQSVLDWYQAQEKNLYIIGG
                     KQIFQAFEPYLDEVIVTHIHARVEGDTYFPEELDLSLFETVSSKFYAKDEKNPYDFTI
                     QYRKRKEV"
     gene            complement(20546..21064)
                     /locus_tag="VYN69_000715"
     CDS             complement(20546..21064)
                     /locus_tag="VYN69_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEV1533027.1"
                     /translation="MVELKKEAVKDVTSLTKAAPVALAKTKEVLNQAVADLYVAHVAL
                     HQVHWYMHGRGFLVWHPKMDEYMEALDGQLDEISERLITLGGSPFSTLTEFLQNSEIE
                     EEAGEYRNVEESLERVLVIYRYLSELFQKGLDVTDEEGDDVTNGIFAGAKTETDKTIW
                     MLAAELGQAPGL"
     gene            complement(21560..23064)
                     /gene="lytC"
                     /locus_tag="VYN69_000716"
                     /pseudo
     CDS             complement(21560..23064)
                     /gene="lytC"
                     /locus_tag="VYN69_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_078138769.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline binding-anchored murein hydrolase LytC"
     gene            complement(23102..23860)
                     /locus_tag="VYN69_000717"
     CDS             complement(23102..23860)
                     /locus_tag="VYN69_000717"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000087908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEV1533028.1"
                     /translation="MSRKPFIAGNWKMNKNPEEAKAFVEAVASKLPSSDLVEAGIAAP
                     ALDLTTVLAVAKGSNLKVAAQNCYFENAGAFTGETSPQVLKEIGTDYVVIGHSERRDY
                     FHETDEDINKKAKAIFANGMLPIICCGESLETYEAGKAAEFVGAQVSAALAGLTAEQV
                     AASVIAYEPIWAIGTGKSASQDDAQKMCKVVRDVVAADFGQEVADKVRVQYGGSVKPE
                     NVASYMACPDVDGALVGGASLEAESFLALLDFVK"
     gene            complement(23958..24635)
                     /locus_tag="VYN69_000718"
     CDS             complement(23958..24635)
                     /locus_tag="VYN69_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HEV1533029.1"
                     /translation="MTYLDAFKSGALVLPSALLLHFKELFPSSDDFLVWQFFYLQNTT
                     GLEEMSPSQIAERIGKEISDVNQSISNLTERGLLQYRTIELNGEIELLFDASLALERL
                     DDLFGAVHSSSDQLTPQNQLKDLVETFQQELGRLLTPFEIEDLTKTLKEDGTSADLIK
                     EALREAVLNGKPNWKYIQAILRNWRHEGIKSVAQIEAKRAEREASNPQLTQVSADFIN
                     AMDLWKD"
     gene            complement(24644..25588)
                     /locus_tag="VYN69_000719"
     CDS             complement(24644..25588)
                     /locus_tag="VYN69_000719"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001122710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HEV1533030.1"
                     /translation="MPIRIDKKLPAVEILRTENIFVMDDQRAAHQDIRPLKILILNLM
                     PQKMVTETQLLRHLANTPLQLDIDFLYMESHRSKTTRSEHMETFYKTFPEVKDEYFDG
                     MIITGAPVEHLPFEEVDYWEEFRQMLEWSKTHVYSTLHICWGAQAGLYLRYGVEKYQM
                     DSKLSGIYPQDTLKEGHLLFRGFDDSYVSPHSRHTEISKEEVLNKTNLEILSEGPQVG
                     VSILASRDLREIYSFGHLEYDRDTLAKEYFRDRDAGFDPHIPENYFKDDDVNQVPCLC
                     WSSSAALFFSNWVNHAVYQETPFDWRKIEDDASAYGYL"
     gene            complement(25770..26282)
                     /locus_tag="VYN69_000720"
     CDS             complement(25770..26282)
                     /locus_tag="VYN69_000720"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEV1533031.1"
                     /translation="MNLKDYIATIENYPKEGITFRDISPLMADGNAYSYAVREIVQYA
                     TDKKVDMIVGPEARGFIVGCPVAFELGIGFAPVRKPGKLPREVISADYEKEYGVDTLT
                     MHADAIKPGQRVLIVDDLLATGGTVKATIEMIEKLGGVMAGCAFLVELDELNGREKIG
                     DYDYKVLMHY"
     gene            complement(26369..27127)
                     /locus_tag="VYN69_000721"
     CDS             complement(26369..27127)
                     /locus_tag="VYN69_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEV1533032.1"
                     /translation="MSEAGHKFLAKLGKKRLRPGGKRATDWLIAEGGFSKEKRILEVA
                     CNRGTTAIELAQRFGCKITAVDMDAQALEVAKKSAGTAGVAHLISFERANAMKLPYQD
                     ASFDIVINEAMLTMQADQAKKKCVMEYLRVLKPGGLLLTHDVLLKEAKESIRQELSQA
                     IHVNVGPLTQDGWEQVMIESGYCDVKVLTGEMTLMKLSGMIYDEGWLGTLKICVNACK
                     KENRKQFLTMYKMFAKNKQKLGFIAMASYKSSKR"
     gene            27335..27481
                     /locus_tag="VYN69_000722"
     CDS             27335..27481
                     /locus_tag="VYN69_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533033.1"
                     /translation="MEIKEQTRKLAVSCSKYIFEVADKTDEISNHTYGKATLTWFEEI
                     FEEY"
     gene            complement(27628..28758)
                     /gene="ugpC"
                     /locus_tag="VYN69_000723"
     CDS             complement(27628..28758)
                     /gene="ugpC"
                     /locus_tag="VYN69_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEV1533034.1"
                     /translation="MVELNLKNIYKKYPNSEHYSVEDFNLNIKDKEFIVFVGPSGCGK
                     STTLRMIAGLEDITEGTASIDGVVVNDVAPKDRDIAMVFQNYALYPHMTVYDNMAFGL
                     KLRKYSKEDINKRVQEAAEILGLKEFLERKPADLSGGQRQRVAMGRAIVRDAKVFLMD
                     EPLSNLDAKLRVSMRAEIAKIHRRIGATTIYVTHDQTEAMTLADRIVIMSATKNPAGT
                     GTIGRVEQIGTPQEVYKNPVNKFVAGFIGSPAMNFINVKLVGSEIVSDGFRLKVPEGA
                     LKVLREKGYEGKELIFGIRPEDVNAEPAFLETFPDCVVKATISVSELLGSESHLYCQV
                     GKDEFVAKVDARDYLQTGATVELGFDLNKAHFFDVETEKTIY"
     gene            complement(29041..29126)
                     /locus_tag="VYN69_000724"
     tRNA            complement(29041..29126)
                     /locus_tag="VYN69_000724"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29090..29092),aa:Leu,seq:aag)
     gene            complement(29208..29783)
                     /locus_tag="VYN69_000725"
     CDS             complement(29208..29783)
                     /locus_tag="VYN69_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEV1533035.1"
                     /translation="MTKALISIDYTEDFVADSGKLTAGAPAQAISDAISKVTRLAFER
                     GDYIFFTIDAHEENDCFHPESKLFPPHNLIGTSGRNLYGDLGIFYQEHGSDSRVFWMD
                     KRHYSAFSGTDLDIRLRERRVSTVILTGVLTDICVLHTAIDAYNLGYDIEIVKPAVAS
                     IWPENHQFALGHFKNTLGAKLVDENLNELSE"
     gene            complement(29783..30571)
                     /gene="codY"
                     /locus_tag="VYN69_000726"
     CDS             complement(29783..30571)
                     /gene="codY"
                     /locus_tag="VYN69_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEV1533036.1"
                     /translation="MAHLLEKTRKITSILKRSEEQLQDELPYNAITRQLADIIHCNAC
                     IINSKGRLLGYFMRYKTNTDRVEQFFQTKIFPDDYVQGANMIYETEANLPVEHDMSIF
                     PVESRDDFPDGLTTIAPIHVSGIRLGSLIIWRNDKKFEDEDLVLVEIASTVVGIQLLN
                     FQREEDEKNIRRRTAVTMAVNTLSYSELRAVSAILGELNGNEGKLTASVIADRIGITR
                     SVIVNALRKLESAGIIESRSLGMKGTYLKVLISDIFEEVKKRDY"
BASE COUNT         8921 a         7100 c         5673 g         9048 t
ORIGIN      
        1 atttctgtat aagttacttc caagccacgc ttaacagctg gacgattgac aattttttct
       61 gcccatttta ctagattttg ataacttgag gcatccaaga attttgcaga accttggtaa
      121 agatttcctt gaactaactg tccataccaa gaccagatag caatatctgc aatcgtatag
      181 tcattgcctg caatataagg tttctgagcc aattccttat ccaataaatc caactggcgt
      241 ttcacttcca tcgtaaaacg gttaatagga tattccaatt tttcaggagc ataattgaag
      301 aaatgtccaa atcccccacc tagaaaaggt gctgcacctg cttgccagaa tagccaattc
      361 aaaacttcta ccttttccac aggattactt ggtaaaaagg ctccaaattt ctcagcaagg
      421 taaagaagaa tatgagcaga ctcaaagact cttacgtttt cagtacctga ctggtccaat
      481 aaggctggaa tcttggaatt tggattgagc ttcacaaagt ctgagccgaa ttgatcccca
      541 tccatgatag caatcttata caagtcgtaa gccgcttcct taaaaccagc ttctagtaat
      601 tcttccaata agatagtaac cttcacacca tttggtgttc ccagtgaata aagctgaaaa
      661 gcttgttctc cttttggcaa gttttgttcg aaacgggcac ctgctgttgg tctgtttagc
      721 cccgtaaaag ctccttggtt actagcttca tcctgccata cggtcggtaa ttgatatgct
      781 gacatccgaa acctccctta aatcgcattc ttgtcaaaac cgagtttgcg ttgaataaac
      841 ttaacgattt cgacgatgat aatcattgag aagcttccag ccataacaat tccccattgt
      901 gacaagtcta gtttggttac gtggaagatt ccttcaagcg gttctacaac gattgttgcc
      961 atgagaagga taaaggatac caagatggac cagttaaagg tcttagactt gaatgggcca
     1021 actgtcaaga tggattggta gacagacttg acattgtagg catggaagag ctgaatcaaa
     1081 ccaagggttg caaaggccat cgttagggca tctgcatgaa tagcatgatt gtcacccaca
     1141 tgaactgggt aagcaatcgc aaggccataa acactcataa caagagctgc ttggagtaca
     1201 ccttgataaa tgatagaact caaaacacca cctgagaaga agcttgcctt gcgtccacgt
     1261 ggtttatgat tcatgacacc aggctccgca ggttcaacac caagagcgat agctgggaag
     1321 gtatccgtta ccaagttgat ccacaaaaga tgaaccggct gcaagacatc ccaaccaaac
     1381 aaggttgata ggaagatggt taatacttca gcagtattag cagaaagtag gtactgaata
     1441 gtcttttgaa tgtttgagaa gaccttacgt ccttcttcca ctgcgacgat aatagtcgca
     1501 aagttatcat ctgcaagaat catatcagaa gcccccttag aaacctctgt accagtgatt
     1561 cccataccga taccgatatc ggctgttttc agagctggcg cgtcattgac accgtcacct
     1621 gtcatggcaa cgactttacc ttgtttttgc caagccttga cgatacgaac cttgtgttct
     1681 ggagacacac gggcataaac agagtattga ccaacgactt tttcaaattc ttcatctgac
     1741 agttcattga gttcagcacc agttaaaacg tgaccttctg tatcgtttgc gtcaatgatt
     1801 cccaaacgtt tggcaatggc ttccgctgtg tcttggtggt cacctgtaat cataattgga
     1861 cggattcccg cttccttagc cacacgaaca gcctcagcgg cttcaggacg ttcagggtca
     1921 atcatcccaa tcaaaccagt aaaaattaaa tcattttcaa gctcttcaga agtgagattt
     1981 tctggaatac tatcgataat cttataagca cctgcaagga cacgcaaggc ttgatgagcc
     2041 atttcagaat tgtttgtatg aatgagattt gtaaccttct catcaatcgg agcaatatcc
     2101 ccagccttat cacgaagaag acaacgtttt aagagttggt ctggcgcacc cttgactgct
     2161 acaaggaaac gaccatctgg caatgggtga actgttgaca tgagcttacg gtcagagtca
     2221 aatggcaatt cagctacacg aggatatttc tctaagaaac ctttgacatc atagcccttg
     2281 tccaaggcat attggataaa ggctgtttcg gttgggtcac caatcaagtt accttccaca
     2341 tcgattttcg tatcattggc caagacaact gaacgaagta gtggcatttc aagacctagt
     2401 tcaatatcat cagctgagtc atgtagaacc gcatcgtaga agactttttc gactgtcatc
     2461 ttgttcatag tcagcgtacc agtcttatca gaagcgatga tttcagttga accaagtgtt
     2521 tcaactgctg gcaacttacg aacgatggaa tgtcgtttgg ccaaaacttg agtaccaaga
     2581 gaaagaacga tggtaacgat agcaggaagt ccttctggaa tggctgcaac ggcaagggca
     2641 acagaagtca acaactcacc aagtggattt ttcccttgaa tgaagacacc cactacaaaa
     2701 gtaacaaggg caatgaccaa gatagcatag gtcaagacct tagaaaggtt gttcaaattt
     2761 tgtttgagtg gtgtatcagt ctcatccgca tcttgaagca taccagcaat atgaccaact
     2821 tcagtgtaca tacctgtatt gacaacaaca cccatcccac gaccataggt tacgtttgag
     2881 ttttggaagg ccatgttgac acggtcacca ataccagcat ctgtcgcaag ctcgactgac
     2941 aagtcttttt cgactggtac agattcacct gtcaaggctg cttcttcaat tttaagagag
     3001 ttggcttcta tcaaacgtag gtccgctggt accacgtcac ctgcttcaag ggcaacgata
     3061 tcgcctggta ccaattcttt agagtcaatc tctgccatgt gtccatcacg aagaacgcgg
     3121 gcaactggac tagacatgga tttgagggct tcaatagctt cttcagcttt tccttcttgg
     3181 taaacaccaa aggcagcgtt gatgataacc acagctagga tgataatggc atctgcgata
     3241 tcttccccac cagaagtcac gactgacaag attgctgccg caactaggat gataatcatc
     3301 aaatccttaa attgctcgat gaatttgacc aagattgatc gtttctcgcc ttcttcgagt
     3361 tcattgtgcc caaattcggc aaggcgcttt tccgcctcac ttgatgacaa accttgctcg
     3421 gtcgcatcca cagcctgcaa gacctcttca gggctctgag tataaaacgc ttggcgtttt
     3481 tgttcttttg acatgtgtct cctccttgac attgtgtgca aaacagactc tctttctgtc
     3541 atatcttttc acgacaaaca aaaagagacc tgttaatcat aacaagtctc gctgtttaag
     3601 atagggccgg aaagcatact tttcagcata aaattcggaa tgacgacact atcacaggtt
     3661 tctgccagct actcccttga gtagtaccat tataccaaat tttggggagt tttcaaagag
     3721 taaaaactgc cttatttgaa tttttccttg aaaaccagta taatggtaga atgctatgtg
     3781 actagaaagg aagttgaatg aagcaatcta tctcaaatct caagttagct gagcgtggag
     3841 ccattatcag tatttcgacc tatttgatct tgtctgcagc caaattagca gctggtcatc
     3901 tccttcattc atccagtttg gtggccgatg gttttaataa cgtatcggac atcattggaa
     3961 atgtggccct cttaatcggg attcggatgg cgcgccagcc tgcagaccgt gaccaccgtt
     4021 ttggtcattg gaagattgaa gatttggcaa gcttgatcac ttctatcatc atgttctatg
     4081 tcggtttcga tgttctaaga gataccattc aaaagattct cagtcgggaa gaatcggtca
     4141 ttgatcctct tggtgcaact ctaggaatca tttctgcagc gattatgttt gtggtctatc
     4201 tctacaatac tcgcctcagt aagaaatcca actccaaggc gctgaaggca gctgctaagg
     4261 acaatctttc tgacgctgtt acctcacttg gaaccgccat tgccatccta gctagtagtt
     4321 tcaattatcc gattgtggat aaactggttg ctatcatcat cactttcttt atcttgaaga
     4381 ctgcctatga tatcttcatc gagtcttcct ttagtctttc agatggcttt gacgaccgcc
     4441 tgctcgagga ctaccaaaag gctatcatgg aaattcccaa aatcagcaag gtcaaatcgc
     4501 aaagaggtcg cacctacggt agcaacatct acctggatat tacactagag atgaatcctg
     4561 acttgtctgt ttttgaaagc catgaaatcg cggatcaggt cgagtctatg ctggaggagc
     4621 gttttggcgt ctttgatacc gatgtccata tcgaaccagc acctatccct gaggatgaaa
     4681 ttttagacaa tgtctataaa aaattgctta tgcgtgaaca attgattgac caaggaaacc
     4741 aactagaaga actcttgact gatgattttg tctatattcg ccaagatgga gagcagatgg
     4801 ataaagaggc ttataagacc aaaaaagagt taaattctgc tatcaaggac attcaaatta
     4861 cttccatcag tcaaaaaacc aaactcatct gctatgagtt agatggtatc atccatacca
     4921 gtatctggcg tcgccacgaa acctggcaaa atatctttca tcaagaaacc aaaaaagaat
     4981 agagaaatcc tttcatgaga cgggattttt ctattctttt atactcaata aaaatcaaag
     5041 tgcaaattag gaagccggtc acaggctgta cttgagtcgg caatgtgaag ccgacatagt
     5101 ttgcactttg attttcgaat agtcttaact atcaaattca ctgagatact catagcgttc
     5161 gtatttttca aggagtgctt catttttctc atccaattct ttttggagag tagccagctt
     5221 accaaagtca gagccgttag cctgcatttc ctcttcaata gcagcgatac gtttttccaa
     5281 ggtttcaata tcaccttcaa tacttgccca ctcctgcttt tcttggtagg tcatgcgttt
     5341 cttgtcttct cgaaccttga ccactttttc cttttcgacc ttttgcactt gattggccat
     5401 atctgtttca aaagcttttt catcaagata gtcggtgtaa tgaccaaaga aaggacgaat
     5461 cttgccatcc tcaaaagcga gaatcttggt cgctacctta tccaagaaat agcggtcgtg
     5521 actgactgtt aaaacgggac ctgcaaaacc ttgcaagaaa ttctctaaga ctgtcaaagt
     5581 tgcaatatct aggtcattgg ttggctcgtc taaaagaaga acatttggtt tttccaaaag
     5641 cagtttgagg agataaagac gttttttctc accccctgac aatttctcaa tcaaggttcc
     5701 atgcgtcgaa cgtgggaaaa ggaactgctc cagcaactct gcaatcgaag tcgtagaacc
     5761 gccactggtc ttgacctcct ctgccacttc ctgcaggtaa ttgataactc tcttgctttc
     5821 atccaaaccc tcaatttgct gagagaaata ggcgatgcga acagtttccc caatcacaac
     5881 ttgtccttta gttggctcaa gacttcctgc aatcaagttg agcagggttg attttccaac
     5941 accattgtcc ccaacgattc cgatacggtc tttggcctgc accaagagat taaaatcttg
     6001 caaaataggc ttgttttcat aggcaaagga aacagcctga aactcgatga ctttcttccc
     6061 aatccgactg gtttcaaagt tcatggtcaa gtctgtctca gcactactgc ctgaaacttt
     6121 ctttttcaga tcatggaatc gattgatacg ggcctgctgc ttggtcgcac gcgcctgcgg
     6181 ttgtctgcgc atccaggcca attcttgttt gtagagttgt tcttttttat gaagtagagc
     6241 tgcatcgcgc tcatcctgtt ccgcctttag gcgaacatag tcctggtaat ttccctggta
     6301 ctcggtcaag cctgcacgat ccaactcgaa aatccgtgtt gacaaagcgt ctaagaaata
     6361 acgatcgtga gtgataaaaa ggacggtctt cttagaattt ttcacaaaga gggtcagcca
     6421 ctcaataatc gcaatatcca gatggttggt cggctcatcc aaaagcaaga ggtcgtggtt
     6481 gccaagtaag acttgtgcca actgtacccg tcttctcaga ccacctgaca attccccaac
     6541 aggagtagat aagtcttgaa tgcccaattt gctaagaacg gtcttgacct gactttcgat
     6601 ttcccaagct tggagagagt ccatctctgc catgacacgt tccaaacgcg cctgcttgtc
     6661 ctcactatag tcgagcataa tcaattcata ctcacgaatg agctggattt ccttgagttc
     6721 actagataga accgtatcca aaactgtctt tctatcatca aaatcaggat cctgagtcaa
     6781 gtaaccaatc tggtaatcat ttttagctga aaaaggactg acatccccat caaatccaga
     6841 aacaccagaa aggacgtcca aaagggtggt cttgccagtc ccattgacac cgattaaacc
     6901 aattctgtct aagtcatgga taataaagga aatatcccta aaaacggtct tgtcaccaac
     6961 ggatttactt agtttttcaa cgataaaatc actcattttt tctccctcag gtaagcatgg
     7021 atggcttcac gattattctc caattctcca tcgacaatgg caaactcaat ctctgttaaa
     7081 atctctccca agtctgggcc tggctgatag ccatattcct tgatcaaaat accgccatta
     7141 atctgaatct ctttcttgtc atggatagtc aagctttggt atttttctgt gatggcttgt
     7201 gggttgactt cttttccttg agcttgacga agattttcag cctgtaaaag caaatctatg
     7261 tcaaagcgat aacaatctcg cttgctcaat tctccatttt cacgcagagc caaaataatc
     7321 agcaaatcct gaacttgctt ggcaaactgg cgtgaggtct tccaagattt caaaaatgac
     7381 tgcgcatttt caatctccaa agcccatagt aaagccgccc aggcttgttc agaggattca
     7441 aaagtaaaat cagtctccaa atcaaacagt ctgttgagct tgtcctggct agatgccata
     7501 tcagggagat agtcataagc ttgactctca atcatggaag ccaagcccct tctccaaaat
     7561 ggagccagca agagtttatc aaattcgacg aaggtacgct ctacagaaat tttctccaaa
     7621 agcggcgtca aggtcttcat agctttaaat gtttctggct caagtgcaaa accaagacta
     7681 gcctgaaaac ggaaaccacg cataatccgt aaagcatctt cgttgaaacg ctcactagcc
     7741 actccaactg ctcgcaagac ttgcttttcc aaatcttcta aaccatggaa caagtcaacg
     7801 atttctcctg tctcatccaa ggcaaaggcg ttgactgtga aatcacggcg tttgaggtct
     7861 tcttctagcg agcgcacaaa ggaaaccgca ctgggtctgc gatagtccac atagacatcc
     7921 tctgtccgaa aggttgttac ctcatactcc tcatccccat ctaagaccaa gacggttcca
     7981 tgctcgattc cgatatcggc tgttcgcgga aaaatctgct tggtctcttc tggataagaa
     8041 gacgtcgcaa tatccacatc gtggataggg ctatggagaa gggcatctcg aacagagccc
     8101 ccaacaaaat aagcctcaaa gcctgcttct ttaatttttt ctaatactgg taaagccttc
     8161 tgaaattcag aaggcatttg cgttaatctc ataataagtg ttctaatcca tagacaagct
     8221 catgacgctt gacaacttct ttaattccca aattgactcc tgtcatgaag gagatgcgat
     8281 cataggagtc atgacggagg gtcaaccctt ctccctgatt gccaaagatg acttcctgat
     8341 gagctaccaa gcctggcaaa cgaactgagt ggatgcgcat accatcaaag tcagcaccac
     8401 gagcaccagc aatcagctct tcctcatctg ctgcaccttg ctgaattgac tctcgaacct
     8461 ctgccatcaa ctcagctgtt ttaatggctg ttccactcgg agcatccttt ttcttgtcat
     8521 gatggagctc aataatctcc acatttggga aatatttggc agcctgcgtc gcaaattgca
     8581 tgagtaagac agcacccaag gcaaagttag gggcaatcag gccacccaag tcttgggcac
     8641 gagaaaattc ttttagctct gcaatttctt cactcgtgaa accagtcgtt ccaactactg
     8701 gagcaaagcc attttcaaga gcaaaacgtg tattttcgta ggcaacagct ggagtagtaa
     8761 agtctaccca gacatccgct tcaaaaccag ctaaatcagc cttatccttg aaaacaggaa
     8821 taccctgcca ttctgactca gactcaaaag gatccaaaac tgctaccaag tccaaatctg
     8881 aatcaggcaa gaccatctgg caagcagctt gacccatctt tcccttaaaa ccggcaataa
     8941 ttactcgaat actcatctct actcctgtct aagatacaaa gcctgtaaga acacaaagtg
     9001 aaaataggaa ttctgatcaa gaagtgtcta ctccttggaa gaactatctt tttcacacag
     9061 ggttccaggc gtgttcaatt atcaaaatac aaaggatctt agccacccat gaaaaatagg
     9121 gaatggcact gactttccac gaaaggcaag acagacatct tttttcaaga ggcaggtagt
     9181 ccgtgttcaa tttctaagat acaaggcatc ttaactagcc tagaagcgcc aactaaatca
     9241 ctggaatata acccagagca atacttcctg atcctaggtg cgttccaatg acactaccaa
     9301 atgtagcaag tgaaacatcc gaacccaagc caaaatcaag caaatgctga cgcaattctt
     9361 cagccttttc aggagcattc ccatgaatga caatgacccg gtattgacct gaagccgttg
     9421 tttccttgat aatttcaatt aagcgcttgg tggccttctt ttcagtacga actttttcgt
     9481 aaacttcaat cacaccttga tcgttaaaat aaaggattgg cttaatgcta agcaaattgc
     9541 ccaaaatggc agccccattt gaaagacgtc caccttttac caaatgatcc aagtcatcta
     9601 ccatgataaa ggctgacgta cggctgattt gaatggctag cttatcctga atgctggcaa
     9661 aatcatcgcc ctgatcacgc caattaaaga cgctttcaac catgatgcct aggggagcac
     9721 ttgtaatcaa agtgtctggg aaagcaatgg ttaagccctc atagtcatcg accatatact
     9781 ggatattttg gtaaaaacct gaaattccag aagataggaa aagccccaag gcatgtgtat
     9841 agccttgttc tttgagcgaa gttaagatct catctaactt ggcaatactt ggttgactgg
     9901 tcttaggcaa ttcagaagcc tgagccattt tttggtaaaa ttcctcagca gacagattga
     9961 tgccttcgac atattcctca ccatcaatat tgacaggaat atccaagaca aacaagtctt
    10021 ctctttgcaa ggtctcttca ctgagataag cagaggaatc tgtgaaaaca gctaatttca
    10081 tattagaact ccaaattaat tcctggtaag tctaatgcaa tttcagtcac ttcgtaagtc
    10141 aaacgattga gcatgttcaa acatggacga gccaaggttt ccacctcttc ttggttcaat
    10201 tcacttggtt cattgacaat acggccatcg atatggttta cttgtgagat tgttccacta
    10261 atgacaaact tatcaaatac aatcataaag ctcaagatga caatcaagga agtcacttga
    10321 ttttcttggt catgttggag caattggaaa ttcacatcca ccttggtttc aggagctcca
    10381 ttttcatttt cccattcaaa attacgcgca tcaaaatgat actgactaac aaattcttgt
    10441 tcacgtttaa gattcatgtc tttctccatc ggctacaata ttataagcta ttgtaccata
    10501 attttttatt ttcatctagt tttctaggat ttagtcaatc ccaatttcag cacgaactac
    10561 atctgtgata gtatcaacat agtagtccac ttcttctgtt gtaggcgctt ctgccataac
    10621 acgcaagagg ggttctgttc cacttggacg aacaaggata cggccgttcc ccgccatttc
    10681 ttcttccatc ttctcgatga tggccttgat agctggcact tccatggcct tttccttcat
    10741 gacgttttcc actcggatat taactaattt ttgtggataa atcgttactt ctgccgccaa
    10801 ctctgataag ctcttaccag tttccttcat gattttagtc aattgaactg ctgataattg
    10861 accatcacct gtggtattgt aatccatcaa gataacgtga ccagactgtt caccaccaag
    10921 gttgtagcct gattttctca tttcttcaac aacgtagcgg tcaccaactg cagtaactgc
    10981 cttgttaata ccttcgcgat tcaaggcctt gtggaaacca aggttagaca taacagttgt
    11041 cacaattgta ttttgagcca attgtccttt ttcagaaagg tattttccga tgatgtacat
    11101 aatcttgtca ccatcaacga tgtcaccatt ctcatcaaca gcaatcaagc ggtcactgtc
    11161 tccatcaaag gccaaaccaa tagctgaccc actttctttg accacttctt gaagggcttc
    11221 tggatgtgtt gaaccaacat taaggttgat gttaagaccg tctggtgttt ccccgataac
    11281 cgtcaattgg gcaccaaggt ctgcaaagat ttgacgggca ctggtagaag ctgctccatt
    11341 agctgtatcc aaggcaacct tcattccatc aagaggagtt ccagttgaaa caaggtatcc
    11401 ttcatactta cgcaagcctt ctggataatc taccaaaatt cctaagcctt ctgcacttgg
    11461 acgaggaaga gtgtcttcct cagcatctag caaggcttca atttctgctt ctttttcatc
    11521 atctagtttg aagccatcac cgccaaagaa cttgattccg ttatcaaggg ctgggttgtg
    11581 gctagcagaa atcatgacac cggcacttgc tccttcagtt tcaaccaagt aagctactgc
    11641 tggtgttgca aggacaccaa gtttgtatac gtgaatccct actgaaagga gacctgccac
    11701 caaggccgat tccaacattt cccctgaaat acgtgtgtca cgtcctacaa agactttcgg
    11761 cgcttccgtt tcatgttgac taagaacata gcctccaaaa cgtcctagtt taaaggctaa
    11821 ttctggtgtt agttctaggt tagcttctcc acggactcca tcagtcccaa aatatttacc
    11881 cattgttata aaatcctttt ctatttttaa ttcgtttttg aactagttgc tttcgttgac
    11941 gaagatgtct ccgatgaact gcttgtactt gaatttgatg tgcttgaact tggtgctact
    12001 ggttttgtag tcaccttcat tattgtatca aacggagtga taactgccgg taagacaaca
    12061 ccattgcggt cgattgcctg caaaggtact gaaccactgt aattacctgt tatacgttcg
    12121 ctagttggca aaacagcgat aatcttatca attctatcca atgtctcttg gtcactcgta
    12181 atagacactt ctttatctga caccatgaca ttttcaattt gtacccgact atcaatttga
    12241 ctagggtcaa tctctggtac aatctttacc ttatccttct gagccttctt accaatcttg
    12301 actgtaattt tttgcggagt cgccacagcg gtcagcccat tgggtaaatc ttcaatgctc
    12361 aaaggaactt caatcgttcc aacaccggca tctgttaggt cagcagtaac cttgaattta
    12421 cgtgtacttt cttgcatttc actagctagc gataggcgat ttgcaccagt caagaccact
    12481 gatacttctg aagcaaaacc gctaataaaa tacttatcac tattatagcg tatgtcaata
    12541 gggacatttg ttactgtatt agtataggtt tccgttttta cctgcctagc actggtactg
    12601 ttttgaaaat tcgtcgccgt agcatagaca aataagacac aagcaaaaaa gagtgaggat
    12661 atgatatata aactattttt tttcatgttt ccatcctcct agcaatcgtt ctttaaaact
    12721 aagacccact tcctcttttg gaagtaagat ttcacgtaat tctgtttcaa attcatcaag
    12781 tgttaggttg tgcttaaacc ttccattata ggttatcgaa attcctcccg tttcctctga
    12841 tacgacaaaa gtcaaggcat ctgagacttc tgataaaccg atagccgccc ggtgtctggt
    12901 cccaaattcc ttggaaatcc ctgtgttttt tgtcaagggc agataggcag acgtcacagc
    12961 gatacgttct tctttgataa tcaccgcacc atcatgtagg ggagtgttgg gaataaaaat
    13021 gttaatgaga agttctgcag aaatcttagc atccaaggga attcctgtcg aaatatactc
    13081 ctgcaaggta cgtacacgct gaatagcaac caaggccccg attttacgag gactcatgta
    13141 ttcaacagac ttaacaaagg cacgaatcat ctgttcctca gcactaatag gggcattgga
    13201 aaagaaatct gtcgctcttc ccaaacgttc caaaccagtc cgaatctctg gagagaagat
    13261 aacaaccgcc gcaataaccc cataagtaat aatttgattg attaaccaag aaatcgtagt
    13321 caaaccaatc atatttgcaa ggatttgagc taaaataaac accaaaactc cacgtaccaa
    13381 aatcataatc ttggttcctg caatagcttt tgtaaaatgg tataaaatat aagcaacaat
    13441 caaaatatca atcagattga tagctatcgt ccatggactt gcaaacaaac tggtccaata
    13501 ttgcagattg gataattgtt gaaaattcat ccctgatatc ctccctatca aaacactttc
    13561 gtcctattat accattttct ggcatttttt tccctatcct agtccatttt acattgaaca
    13621 aaaatatgat aaaataaact gactaaaaaa aacaaaggag aaactatgtc tcaactctat
    13681 gatattacca ttgtgggtgg tggtcctgtc gggctttttg cagcctttta tgcccaccta
    13741 cgccaagcca aggttcaaat catcgactct cttccccagc taggtggaca acctgctatt
    13801 ctctaccctg aaaaggaaat cctagacgta ccaggcttcc caaacctgac tggagaagag
    13861 ttgactaacc gcttgattga acagctaaat ggatttgata cccctattca tctcaatgaa
    13921 acggttcttg agattgacaa acaagaagaa tttgccatca caacttctaa aggaagtcac
    13981 ctgactaaaa cagttatcat cgctatgggt ggcggtgcct tcaaaccacg tccgctggaa
    14041 cttgaagggg ttgagggcta tgaaaatatc cactaccacg tttctaacat tcagcaatac
    14101 gctggtaaga aagtgacgat tcttggtggg ggagactcgg ctgtggattg ggctttggct
    14161 tttgaaaaaa tcgcaccaac tacccttgtt caccgcagag ataatttccg tgccttggaa
    14221 cacagtgttc aagccttgca agaatcatct gtaaccatca agacaccatt cgcccctagc
    14281 caactccttg gaaatggaaa aacacttgat aaacttgaaa tcacaaaagt caaatctgat
    14341 gaaactgaaa ccattgacct agaccacctc tttgtcaact atggtttcaa atcttctgtc
    14401 ggtaacctta aaaactgggg gctcgacctc aaccgtcaca agattatcgt caacagcaaa
    14461 caggaatcca gccaagcagg tatctatgct atcggtgact gctgctacta tgacggaaaa
    14521 attgatctga ttgcgacagg cctcggagaa gctccaactg ctgtcaacaa cgctatcaac
    14581 tacattgacc ctgaacaaaa agtacaacca aaacactcta ctagtttata aaaaagaacc
    14641 acgagtcaca taggattcgt ggttttataa ttcatccgct atcttattga tttttctgag
    14701 tctgtgattg acaccacttt tggtcagagg ggtgctgaga ctatctgcta actgctggat
    14761 agagtagtct gggtgctgaa tcctcagttg cgccacttcc tgcaaatcta ctggcaaatt
    14821 ttctaagccc atgatatctt tgattttact gatattgtta atggtcttca tgctggcaga
    14881 aactgtccga gcgatattag ctgtctcggc attattagcc cgattgaggt cgttacgggt
    14941 ttctcgcaaa atcttaaccc gctcaaaatc atcacgtgcc tgcatggctc ctattactat
    15001 caagaagtcc ataatgtctt ctgctcgctg gagataggtc acagccccct tcttgcgctc
    15061 aagcaccttg gcatccagta aaaactgttg gagaagggag gcaattcctt gcgcgtggtc
    15121 cagataaaca gaactgattt ccaactggta cttgcctgac tcagggtcac gaatgctccc
    15181 atttgccaag aaagcgccac agagataggc acgacctgct tcctcatccg ataaaatcgc
    15241 ctcatcaata cctgtttcca ggccaaagaa agagtctgcc aagtgcaaat cacttaacaa
    15301 atcctgcacc ttttcatctg taaaaacggt atagacgcga ttcttgcgaa gattgctccg
    15361 ttggtggtga cgaatttcag atttgatttc atagaaatgg agaaaggact catagaggtg
    15421 acgggccagt ttggcatttt ctgtcacaac tgacaaagtc aagcccgaag tcgagagacc
    15481 gatgctacca gacattttga taatggcaga taattcatgc cagctcagat ggtgttggcc
    15541 caggatttct tcttttactg ctactgtgaa actcattttt tcacctgtat aatgcgcatc
    15601 aactcgtcca caatcaaatc tccatcgtgg aaggcaccgc cattttccag acgaaggaag
    15661 ttagatgaaa tcacgcgcga aacttgctta caaagaccta caaaatcgtg ttccacttgc
    15721 accaagtatt catcaaaacg gttggaattc atgtattcct gaggcacttt ttcaatattc
    15781 accaagacag tgtcgataaa agggcgacca aggtgacgat gcaagacttc cacgtggtcg
    15841 ctatctgtaa agtgttccgt ctccccacgt tgggtcatga tattgcagac ataggcaatt
    15901 tctgccttgg tttccaaaag agcccgccca atttccttaa tcacgatatt gggcaaaata
    15961 gaggtaaaga gggaacctgg ccctaggaca atcatgtcac tttcaaggat ggtctgcact
    16021 actcgacggc tggccagagg cgtatcatcg tttagggcat tggtcacata gacattgtca
    16081 attatgcctc gatggtctac aatatgactc tctccagcca cttctgtccc atcctgaaag
    16141 actgcatgaa gggtcaaagg atggtcactg gaaggataaa ttttccctgt tgtatggaaa
    16201 aatttgctca ataactgcat ggcattatag gttgaaccct gcatttctga caagccagca
    16261 atgatgagat ttcccaatgg atggccagca aaggctccgg catcctcaga gaaccgatac
    16321 tgaaagacct tctcataaaa cttaggcata tccgacatgg ccacaaggac attacgaaga
    16381 tcacctggcg gtgtcaactg ttgcatattt tttcggagtt cacctgaaga accaccatca
    16441 tctgccaccg tcacgatagc tgcgatttcc acatcttttt cccgcagact ttttagaatg
    16501 acgggaattc cagtccctcc accaatcacc gttatctttg gttttctcat gaacggttta
    16561 ccgtttcctt tctgcggtct ttgtcgcgat gcccttcatt aacagaccaa ttcttggata
    16621 agtcctgcac caagcgttta gcaaatgcca cactacggtg ttgtccaccc gtacatccca
    16681 tggcaatggt caaaacggac ttaccttcct tttggtaact tggcagaatc ggctcaatca
    16741 aggccaataa atgttgataa aagtcttctg actcaggatg gttcatgaca taatcataaa
    16801 caggttcatc cacacccgtt tggtttctca gttctggtaa ataataggga tttggcaaga
    16861 aacggacatc aaagaccaag tccgcatcaa tcgggattcc atacttaaat ccgaaagaca
    16921 tgacttcgat acggaaagac tgggcttgtt cttggtctga aaactgctct gcaagggttt
    16981 tgcgcagctc acgtggagtg agttcagtcg tatccaccac attttggctc atatttttca
    17041 aaggtgccaa gagttcacgt tccaacttga ttccatctaa aatacgaccg tctgctgcta
    17101 gtgggtgact ccgtctggtt tccttgtaac gagcgaccaa ttccttatca gccgcatcca
    17161 aaaagaggat tttgaaatcc aaaccatctt gattttccaa ctcatccaaa acagcttgaa
    17221 tctctgaaaa gaaagaacgg ctacgcatat ccactaccaa ggccaactta ggattgtctt
    17281 ccttaatttc aaccagctgc aaaaacttag gcaagagagc tggcggcata ttatcaatgg
    17341 tgaaataacc tagatcctcg aaggactgaa tggctacagt tttccctgcg ccactcatcc
    17401 ctgtcacaat caccaagtga agttgtttct ttgtcatctt tttctcctta tatcaaaaga
    17461 agtttggcaa caccaaactt caactagctt atccaatctc tgcgatgact tcaatttcga
    17521 cttttacatc acgaggaaga cgagctacct ccacagctga acgagctggg aattcctctt
    17581 tgaaggccgt ttggtaaacc tcattaaaag gaacaaagtc gttcatatcg ctcaagaagc
    17641 aagttgtttt gacaacatgg tcaaagtctg ttcctgcttc tgccaaaata gcaccgatgt
    17701 ttttcaagac ttgctctgtc tgttcttgga tattctctcc tacaatttcc ccagtttcag
    17761 gggatagggg aacttgaccg ctagcaaaca aaaggttgcc aacgattttt ccttgaacat
    17821 agggcccgat agcctttggg gccttatctg tatgaattgt ttttgccatt ttcttttcct
    17881 cacaattttt ctaagattgc atcccaagcc tcatccatcc ctgccttgct gacagatgaa
    17941 aagaggatga aatcgtcact tgggtcaaag tttaatttct ttttgattgc tgattcatgc
    18001 ttgttccatt taccacgagg aatcttgtcc gccttggtcg ccacaatgat gactggaatc
    18061 tcataatact tgagaaattc gtacatctgc acatcatctg ctgacgggtc atgacgaagg
    18121 tcaactagac tgacaaccgc acggagattt tcccgagtcg ttaagtactc ctcaatcatg
    18181 cacccccact tttcacgttc ctttttagaa acacgagcat agccataacc aggcacatcc
    18241 acaaagcgca tcttgtcatc aatgttaaaa aagttcagga gctgggtttt accaggtttt
    18301 cctgatgtac gagcgagatt cttacggttc aacatagtgt tgataaagct ggatttacca
    18361 acatttgaac gccctgctag ggcaatctct ggcagttcat cctgcggata gtgggactta
    18421 ttagctgcac tgagcaagat ttcagcattg tgtgtattaa gttccatagt cacctctagg
    18481 ctgtttctag gatcggttta tccgttccat cgacagtttc tttagtgatg cgaaccaatt
    18541 tcacattttc ctgactcggc acctcaaaca tgacatctag catggtttct tcgatgatgg
    18601 agcgaagtcc acgcgcccct gtcttccgtt cgattgcttt attagcaatc tcttgaaggg
    18661 cttcgtcgtc aaattccaac tcaacatcat cataagaaag caaggtttgg tattgtttca
    18721 ccaaggcatt tcttggctct ttcaagatgc gaaccaagtc atcaacggtc aattgctcaa
    18781 gagccgcaaa aacaggcaag cgtccaatca actcagggat aataccaaat ttttgaatgt
    18841 cttcagcgat gatttcttgc atatatgagc tgttttcatc aatcgcctta ttgttttgac
    18901 caaatccgat gactttttca cccagacgtt gtttgacaat ttcttcaata ccatcaaaag
    18961 caccacccac gatgaagagg atattttttg tatccacttg aatcatctct tgttgtggat
    19021 gtttgcgtcc accttgaggc ggcacgctag caacagttcc ctcgataatc ttgagaaggg
    19081 cttgttgcac cccttcacca gaaacatcac gtgtgataga cacattctca ctcttcttgg
    19141 caatcttgtc aatttcatcc acatagataa tgccacgctc tgcacgttcg atgttaaagt
    19201 cagcaacctg caagagtttg aggaggatat tttccacatc ctcacccaca taaccagcct
    19261 ccgtcagagc tgtcgcatcc gcaatagcaa aaggtacatt caagctctta gccaaggtct
    19321 gggcaaggaa agttttccct gaaccagttg ggccaatcat caaaatgttt gacttctgca
    19381 aatccacatc ttctgactct tcgcgtgtat cgtggaaatt gatgcgtttg tagtggttat
    19441 aaaccgccac tgccaaggca cgcttggcac gatcttggcc aattacatag tggttcaaga
    19501 tatggaggag ttcaattggt tttggcacct cagacaagtc tgccaagact tcctcaacca
    19561 attcttctcg aatgatttcc tgagctaact ccacgcattc attacaaata aaagcattgt
    19621 tgccagcaat tattttttgt acttcttctt ggtttttgcc acaaaatgag caataaacca
    19681 tcatatcatt ttttctattt gtagacatga tttccttcca ttctatactg tcattctatc
    19741 taaaataagg tcatgtaaaa agcatgaata ctattgacca gattggtaaa ggcatttaac
    19801 caaaggagga tagaaagccc gtaacgcttt ttacgaaaag cttgtgctcc tgccagaaag
    19861 cagatgaaac acagaaaagc cgtgaataga ccaaataaac tccgttccat tagacttcct
    19921 ttctcttgcg gtattggatg gtaaaatcat aaggattctt ctcatctttg gcgtaaaatt
    19981 tgcttgaaac tgtctcaaaa agagacaagt caagttcttc agggaaatag gtatctcctt
    20041 ccacccgagc atgaatgtga gtgacaatca cttcatcaag gtaaggttca aaagcctgaa
    20101 aaatttgctt cccaccgata atgtagagat tcttttcttg agcctgatac cagtcaagaa
    20161 cagactggac gtcctgaaaa gtagcaaccc catctatctt ttcttccgga ttacgcgtca
    20221 aaatcaaggt ttcccgtttt ggaagcaagc gacgccccat cccatcaaag gtcacacgcc
    20281 ccatcaagat agcatgattc agagttgttt ctttaaagtg ctgcaattct gctggcaaat
    20341 gccaaggcag acgattttcc ttaccaatca caccctcttc atcctgggcc caaatagcta
    20401 cgattttctt agtcatgctt ccatcctttt cactgatagt actattttat caaaaaactc
    20461 aaaaaaagac tggtttggaa tagcttacaa aatagaaaaa atctgtaaga aatttcctac
    20521 agatttatct atgtttcctt atttcttaca aaccaggtgc ttgtccaagt tcggctgcaa
    20581 gcatccaaat tgttttatct gtttcagttt tagcgcctgc aaagataccg tttgtcacat
    20641 cgtcaccttc ttcatcagtg acatccaaac ctttttggaa aagttctgac aagtaacggt
    20701 agataacaag aacacgttcc aagctttctt caacattacg gtattcacca gcttcttctt
    20761 cgatttcact attttgaagg aactctgtca atgtagagaa tgggcttcca ccgagtgtaa
    20821 tcaagcgttc actgatttca tccaattgac cgtcaagagc ttccatgtac tcatccattt
    20881 ttggatgcca tacaaggaaa ccacgaccat gcatatacca gtgcacttgg tgcaaagcaa
    20941 cgtgagctac atacaaatca gcaacagctt ggttcaagac ttcctttgtt tttgccaatg
    21001 ctactggcgc tgcttttgtc aatgatgtta cgtcttttac tgcttctttt ttcaattcta
    21061 ccattttctt tacctcattc attatttttt gtaaccactt cttaatgatt actaccttag
    21121 tatactacta aggaaaacca atagcaagac aaagaactta tatgagaaaa gttgcaatag
    21181 actgataatt taagaattct ttagaagaca aaaagagggt tggatgctta tagtcaatgg
    21241 aaatccatca gcaaactagg aaactaaacc cagcttgacc aaagcacaag ttttgaggtc
    21301 gcaggtagaa ctgaccaagc tgatgtggtt tgaagaaatt ccccaatact ataatagatt
    21361 gaaataagat gaaaccaagt cgattaggaa aggtaaatga atttctaaaa tgttttacca
    21421 atcacaaggt atttgtctat attcaatcta ctatataagc ttcatttccg agcaagaact
    21481 tgaatttact cgaggatgaa ctatcataaa acaaagaaaa agagggtact actatcacat
    21541 ccctctttca aatcatcgct taataccaaa cgctgacatc tacgcgccct tggattcctt
    21601 tcatgtattc agaagaggta tattgccaac ctttttttcc tgaataatga gggttttccc
    21661 attctaaagc attcgtatag gccgctaccc agtttacatg ttttaaaata tctgggtgtt
    21721 ttaaacgcgt ctgtaataaa ctacgatagc tatagacata cacattttga taacccgcct
    21781 gcttcatcgt gtccatgtac ttgttgatga ttttaaccca agtacctgta tcacttggag
    21841 ctctcttgct cttatttaca tattcccaat tctcaacatc ataatagata gggtaagaca
    21901 ggttcatatt gtatttcttt ataagttcaa tggtctgttt agcgtcactc tcagcatcgg
    21961 tctcattttc agcataggta tagagataga caccataagg aattcccaga cggtttaact
    22021 ccttaatgtt atgcgccaat tccttgtctt ctttaccgct ataacctaga cgaacaatga
    22081 caccatccac ttcgttctca tcaataacct ttttccaatc attgatacga ccattgtgct
    22141 cactaatatc aatgactttc ttagcatgtt cggttcccac ttgttcttct ctattattaa
    22201 agaaatagcg cttaccatct ctgtgaaccc agccgacatt caaatctttt ttttctttga
    22261 gctcaccaga ggccgcaaag atatagcgag taccatccat aatcacttcg tcagtcgcca
    22321 tggcaccact tccagtcaaa tagtagttgc cttgccactc attccgagcc atatagcccc
    22381 acttcttgaa atagtaccat ttgccgccca ctttttgcca ctcttggtta gcataagttc
    22441 catcggattt tagataaaaa taagcagaat aggctggatc atagagccat tcattttgca
    22501 tcatagctcc ttgaccattc aagaaataac ttccttgcca ttggctttta gccatgtaac
    22561 cccacttctt gaagtagtac cacttatttc caatcaattg ccattcttga tgagcataag
    22621 ccccatctga cttgagataa aaccaactct tgtaattact gtcatagatc cactcgtttt
    22681 gggccatata tccacctgat ttcaggtagt aatttccatg ccattcattc tgcgagtaac
    22741 gaccatctga attaatataa aaccaagcct tatagtagtt gtcaaaaatc cattcacttt
    22801 gagctttaga accatcatcc tttacataat aatccccatc ccagtgggca gcagcattag
    22861 tctgtacttc tgcgctcgtt tgcgttttat tagaagactg attttgctct gaactacttg
    22921 aagctgtcgt tgtatcctgc gaagtttttg ctacttcagt ttcatttgca gcgacatgac
    22981 tagttgccaa gcctagtaaa cagatacttg ctaatccaat ttttattttc aatctttctc
    23041 tcctataaaa aatgtaacag acatctgaat gctgttccac ctagcttttg ctacttactg
    23101 attattttac aaagtcaagc aaagccaaga agctttcagc ttcaagtgac gcaccaccta
    23161 caagggcacc gtcaacgtct gggcaagcca tgtatgaagc aacattttca ggtttaacag
    23221 aaccaccgta ttgaacacga actttgtctg cgacttcttg accaaagtca gcagctacaa
    23281 cgtcacgaac aactttacac attttttgtg catcgtcttg tgaagctgat ttaccagtac
    23341 cgatagccca gattggctca taagcgataa ctgaggcagc aacttgttca gcagtcaatc
    23401 cagccaatgc agcagatact tgagcaccta cgaattcagc agctttacca gcttcgtaag
    23461 tttcaagtga ttcaccacaa cagatgattg gaagcatacc gttcgcaaag attgcttttg
    23521 cttttttgtt gatatcttca tcagtttcat ggaagtagtc acggcgttct gagtgaccga
    23581 taacaacgta gtcagtaccg atttctttca aaacttgtgg gctagtttca ccagtgaaag
    23641 cacctgcatt ttcaaagtag cagttttgag cagcaacttt aaggtttgag ccttttgcaa
    23701 cagcaagaac agttgtcaaa tcaagagctg gagcagcgat acctgcttca acaagatctg
    23761 atgaaggaag ttttgatgca actgcttcaa cgaatgcttt agcttcttct ggatttttgt
    23821 tcattttcca gttaccagcg ataaatggtt tacgtgacat ttcacatacc tcttttttca
    23881 atttattttc tatttattct atcacaatta tacacagctt gcaaatctta ctcggatttc
    23941 aagcctactt gcatggatta atccttccag agatccatgg catttatgaa atctgcagat
    24001 acctgtgtca actgaggatt gcttgcttct ctttctgctc tcttggcctc aatttgagcc
    24061 acactcttga ttccttcatg gcgccagttt ctcaaaatcg cctgaatgta cttccagttt
    24121 ggttttccat tcaaaacagc ttcacgaaga gcctccttaa tcaagtcagc actggttcca
    24181 tcttccttta gtgtcttggt caaatcctca atctcaaaag gcgtcaacaa tcgtcccaac
    24241 tcctgctgga aggtttccac caaatccttg agctggtttt gaggtgttag ctggtctgaa
    24301 cttgaatgaa ctgctccaaa caggtcatcc aaacgttcca aggccaaact agcatcaaag
    24361 agcaattcaa tttcgccatt taattcgata gtacgatact ggagcagtcc cctttccgtc
    24421 agattagaaa tggactggtt gacatccgaa atttccttgc caatcctttc agcaatctgg
    24481 cttggcgaca tttcttctaa gcctgtcgta ttttgcaaat agaaaaattg ccaaaccaga
    24541 aaatcgtcgc tagaaggaaa gagttcctta aaatgcaaga gcagggcact cggtaaaacc
    24601 aaggcacctg atttaaaagc gtctaaatat gtcataattc ctcttataaa tacccatatg
    24661 cagatgcatc atcttctatc tttctccagt caaaaggcgt ctcctgatag accgcatggt
    24721 ttacccaatt actgaaaaag agggctgcag atgaagacca acaaagacaa ggtacctgat
    24781 taacatcatc atctttaaag taattttctg gaatatgtgg gtcgaaacct gcatcacgat
    24841 ctcgaaaata ctcttttgcc aaggtatcac ggtcatactc caaatgacca aaactataaa
    24901 tttctcgtaa atcacgactg gccaaaatag aaaccccaac ctgaggtcct tctgataaaa
    24961 tctcgagatt ggtcttgttt aagacctctt ccttagaaat ctccgtgtgc cgtgaatgag
    25021 gggatacata gctatcatca aagcctctaa atagaaggtg accctctttt agggtgtcct
    25081 gaggataaat acctgatagc ttactgtcca tctggtattt ttctacacca tagcgcagat
    25141 aaagcccagc ctgagccccc caacagatat gaagggtcga atagacatga gtcttagacc
    25201 actcaagcat ctgtctaaat tcctcccaat agtccacttc ctcaaatggt aaatgctcaa
    25261 ctggagcacc cgtgatgatc atcccatcaa aatactcatc cttgacttca ggaaaagttt
    25321 tatagaaggt ctccatgtgc tctgaacgag ttgttttaga acggtggctc tccatataga
    25381 gaaaatcaat atccagttgt aggggtgtat tagccaagtg gcgcaacaac tgggtctctg
    25441 tgaccatttt ctgtggcatg agatttaaaa ttaaaatctt caaaggacgg atatcttggt
    25501 gggcagcacg ttgatcatcc atgacaaaga tattctctgt ccgtaaaatc tcaacagctg
    25561 gcaatttttt atcaattcga atcggcataa ccctctccta actgtactct ttcaggaata
    25621 attgcatatg tttgaagaca aatctcaaac acttagtcct tttattatac tgcaagaaga
    25681 tatagttttc aattatagtt tttctctaac tagttatagt ctatttttat atcctagtgt
    25741 agagaaaaca gccctaggga ctgttttcat taataatgca taagaacttt gtagtcgtag
    25801 tcaccaattt tttcacggcc gttcaattca tccaattcaa caaggaaggc acaacctgcc
    25861 ataacaccac caagtttttc aatcatctcg atagttgcct taacagttcc acctgtcgcc
    25921 aaaaggtcat ctacaataag aacacgttga cctggcttaa tggcatccgc gtgcatagtc
    25981 aaggtatcga caccgtactc tttttcatag tcagcagaaa taacttcgcg tggcaattta
    26041 cctggcttac gaacaggcgc aaaaccaatt cccaactcaa aggcaactgg acaacccacg
    26101 ataaatccac gagcttcagg tcccacgatc atgtcgactt tcttgtcagt agcatactga
    26161 acgatttcac gaacagcgta gctataagca tttccatcag ccatcaaagg actaatatca
    26221 cggaaggtaa tgccttcctt tggataattt tcaattgttg caatgtaatc ttttaaattc
    26281 atctttttct ttctttcaaa gttttttact ctctattata gcatattttt taagaaagaa
    26341 aaaaggaaaa gttaacttca ataattatct aacgttttga cgatttataa ctagccatcg
    26401 caataaagcc caatttctgt ttattcttag caaacatttt atacatagtt aaaaactgct
    26461 ttctattctc ctttttacaa gcatttacac aaattttcaa agttcctagc caaccttcgt
    26521 cataaatcat acccgataat ttcattaatg tcatttcacc agtcaatact ttcacatcac
    26581 aataacctga ttctatcatc acctgttccc aaccatcttg agttaaagga cctacattta
    26641 catgaattgc ttgtgataat tcctgtctga tagactcttt agcttcctta agaagcacat
    26701 catgtgtcaa aagaagacct ccaggtttta atacccttag atattccatt acacattttt
    26761 tcttagcttg atcggcttgc atagtcagca tagcttcatt tataacaata tcaaaactag
    26821 catcttgata aggaagtttc attgcatttg ctctttcaaa actgattaaa tgagcaacac
    26881 ctgccgttcc agcagatttt ttagccactt ctaaagcttg agcatccata tcaacagcag
    26941 ttatcttgca accaaaacgc tgtgccaact caattgctgt agttccccta ttacacgcaa
    27001 cctctagtat tctcttttct tttgaaaatc ctccttctgc aattaaccaa tctgtggcac
    27061 gctttccacc tggacgtaag cgttttttcc ccaattttgc taaaaactta tgacctgctt
    27121 ctgacatttg cacacctctg ttttttcttc attataacac aaaatgatat tatctgacaa
    27181 ttctaatcta ttatttgata aactagaatc aaaagcatac aagtcaccat aactatagca
    27241 taaatttatt attacctctc tagttcgaat tcaattgctc acgtagttta cgcatatagc
    27301 ttcacttgta gatggtaccc acaaataata ctcaatggaa atcaaagagc aaactaggaa
    27361 actagccgta agctgctcaa agtacatctt tgaggttgca gataaaactg acgaaatcag
    27421 taatcatacg tacggcaagg cgacgttgac gtggtttgaa gagattttcg aagagtatta
    27481 aagagcatca caatatagat gcataatatg acttccctag caaaaaggag agaagcattt
    27541 tgtttctctc cctagattaa ctgatatctc tccactacaa ttgataaaga gatatctttt
    27601 cttgtagtgc tttgaatttt atttatttta gtaaattgtt ttctcagttt ctacatcgaa
    27661 gaagtgtgct ttgttcaagt caaatccaag ctcaactgtt gcacctgttt gcaagtagtc
    27721 acgagcatca acttttgcaa caaactcgtc tttaccaact tggcagtaaa ggtgagattc
    27781 tgaaccaagc agttctgata cagagatagt cgcttttaca acacagtctg ggaatgtttc
    27841 aaggaaagca ggttctgcat tcacgtcttc tggacggata ccaaagatca attcttttcc
    27901 ttcgtagcct ttttcacgaa gaactttcaa tgctccttct ggcactttca aacggaaacc
    27961 gtcagaaaca atttcgctac caaccaattt cacgttgatg aaattcatag ctgggcttcc
    28021 gatgaatcct gcaacaaatt tgttaactgg atttttgtaa acttcttgag gagtaccgat
    28081 ttgttctaca cgtccgatag tacctgtacc agcagggttc ttagtagctg acataataac
    28141 gatacggtct gcaagtgtca tcgcttctgt ttggtcgtga gttacataga tagttgtagc
    28201 tccgatacga cggtgaattt tagcgatttc agcacgcatt gatacacgaa gtttggcatc
    28261 caagtttgac aaaggttcgt ccatcaagaa tactttcgca tcacggacaa tcgcacgccc
    28321 catggcaaca cgttgacgtt gaccacctga aaggtcagct ggtttacgtt ccaagaattc
    28381 tttcaatcca agtatttcag ctgcttcttg aacacgtttg ttaatgtctt ctttgctgta
    28441 tttacgcaat ttcaaaccga aagccatgtt gtcataaaca gtcatgtgtg ggtaaagagc
    28501 gtagttttgg aataccatgg cgatatcacg gtcttttgga gctacgtcgt tgacaaccac
    28561 gccatcgata gatgcagtac cttctgtgat gtcttcaaga ccagcaatca tacggagtgt
    28621 agttgattta ccacatcctg aaggtcctac gaaaacgata aattctttat ctttgatgtt
    28681 caagttgaaa tcttcaactg aatagtgttc gctgtttgga tattttttgt aaatattttt
    28741 aagattcaat tctaccatga ggtgaactcc ttttgtcttt tgataattat attatagatg
    28801 aaaacgcttt atatttctat ggcaagatga ccaaaaaaat aaaaaagttc tgtgcaactt
    28861 gcacaaaact ttagagaata tcggagaatt tgaagaagat aatctttatt attcttctat
    28921 gacaaaagca gaaacaagga tcagttttcc catattttcg ctgattctcc actacatttg
    28981 actggattct aattttttag agaaatacaa aagagctagc tttagctaac tcttttccta
    29041 tgcggagaga gggacttgaa ccctcacgac ctaaagcggt cacaggatcc ttagtcctgc
    29101 gcgtctgcca attccgccat ccccgcatcg attactttac tagtatatca acttttggga
    29161 tgcttgtcaa cacttttttt caaatttttt cattttcacc aaccaggtta ctcagaaagt
    29221 tcatttagat tttcatctac taacttagct ccgagtgtat ttttgaaatg acctagggca
    29281 aattgatgat tttcaggcca gatggaagca acagctggtt taacaatctc gatgtcatat
    29341 cctagattat aggcatctat agctgtatgt aggacacaga tatccgtcaa gacacctgtt
    29401 aagataacgg tagacactct acgctctctc aaacgaatat ctaggtcagt ccctgaaaaa
    29461 gctgagtaat ggcgtttatc catccaaaag acacgactgt ctgaaccatg ctcttgataa
    29521 aagatcccca aatctccata taaattccgt ccactcgtcc caatcagatt atgaggagga
    29581 aataacttac tttccggatg gaaacaatcg ttttcttcat gagcatcaat agtaaagaag
    29641 atataatctc ctcgttcaaa agctaatcga gttaccttgc tgatggcatc cgaaatcgcc
    29701 tgagctggag cacctgctgt cagcttccca ctatcagcaa caaaatcttc tgtataatca
    29761 atcgaaatta aagcctttgt cattagtaat ctcttttctt cacttcttca aaaatatctg
    29821 aaatcaagac cttaagatag gttcccttca ttccaagtga gcgactttca ataatccccg
    29881 cagactcaag tttacgaaga gcattgacaa tcacagagcg agtgattccg atacgatctg
    29941 caatcactga cgcagtcaac ttcccttcat ttccatttaa ttcccctaaa attgctgaaa
    30001 cagcacgaag ttcggagtaa gaaagggtat tgaccgccat ggtgacagca gtacgacgac
    30061 gaatattttt ctcatcttct tcacgttgga agttaagaag ctgaatccca acaacggtac
    30121 tggcaatctc aacaagaacc aagtcctcat cttcgaattt tttatcatta cgccaaataa
    30181 tcaaagaacc aaggcgaatc cccgatacat gaatcggtgc aatagtcgtc aagccatctg
    30241 gaaaatcatc tctactctca acagggaaaa tactcatatc atgctcaaca ggcaagtttg
    30301 cttctgtttc gtaaatcata ttagcccctt gaacgtagtc atctgggaaa atcttagttt
    30361 ggaagaattg ctctacgcga tctgtatttg ttttataacg cataaaatag ccaagcagac
    30421 gtcccttact attgataatg caggcattgc aatgaataat atccgctaac tgacgcgtaa
    30481 tagcgttgta agggagctca tcctgcaact gctcctctga gcgtttcaaa attgatgtaa
    30541 tttttctagt tttttctaat aaatgtgcca tttttcacct cgaatttaat cgctatcatt
    30601 ataacataaa aacgtctctt tttcaataat tatctgaaaa ttccttattg acttgcattg
    30661 acttacaatt taattaaaaa ccagaatatt tttaattaaa ttgttccttt tctattgaca
    30721 agttgcctat ttttgtgtat ca
//
LOCUS       DAVLVP010000017        27518 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.017, whole genome shotgun sequence.
ACCESSION   DAVLVP010000017 DAVLVP010000000
VERSION     DAVLVP010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27518
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.017"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(537..629)
                     /locus_tag="VYN69_000727"
     CDS             complement(537..629)
                     /locus_tag="VYN69_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEV1533037.1"
                     /translation="MMELVLKTIIGPIVVGVVLRIVDKWLNKDK"
     gene            790..1761
                     /gene="ruvB"
                     /locus_tag="VYN69_000728"
     CDS             790..1761
                     /gene="ruvB"
                     /locus_tag="VYN69_000728"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000502070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEV1533038.1"
                     /translation="MGDEELVERTLRPQYLREYIGQDKVKDQLQIFIEAAKMRDEALD
                     HVLLFGPPGLGKTTMAFVIANELGVNLKQTSGPVIEKAGDLVAILNELEPGDVLFIDE
                     IHRLPMSVEEVLYSAMEDFYIDIMIGAGEGSRSVHLELPPFTLIGATTRAGMLSNPLR
                     ARFGITGHMEYYAHADLTEIVERTADIFEMEITHEAASELALRSRGTPRIANRLLKRV
                     RDFAQIMGNGVIDDIITDKALTMLDVDHEGLDYVDQKILRTMIEMYSGGPVGLGTLSV
                     NIAEERETVEDMYEPYLIQKGFIMRTRSGRVATAKAYEHLGYEYSEK"
     gene            1742..2314
                     /locus_tag="VYN69_000729"
     CDS             1742..2314
                     /locus_tag="VYN69_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HEV1533039.1"
                     /translation="MNTVKNKQEILEAFRENPDMMAILTIIRDLGLKDSWLAAGSVRN
                     FIWNLLSDKSPFDHETDIDVIFFDPDFSYEETLLLKKKLREDFPQYQWELKNQVYMHQ
                     HSPHTASYTSSRDAMSKYPERCTAVGLRLNEESDFELYVPYGLEDILNFQVRPTPHFL
                     ENEDRMELYQTRLSKKNWQEKWKNLIFKNT"
     gene            2545..3303
                     /locus_tag="VYN69_000730"
     CDS             2545..3303
                     /locus_tag="VYN69_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEV1533040.1"
                     /translation="MFGFFKKDKAVEVEVPTQVPAHIGIIMDGNGRWAKKRMQPRVFG
                     HKAGMEALQTVTKAANKLGVKVITVYAFSTENWTRPDQEVKFIMNLPVEFYDNYVPEL
                     HANNVKIQMIGETDRLPKQTFEALTKAEELTKNNTGLILNFALNYGGRAEITQALKLI
                     SQDVLDAKINPGDITEELIGNYLFTQHLPKDLRDPDLIIRTSGELRLSNFLPWQGAYS
                     ELYFTDTLWPDFDEAALQEAILAYNRRHRRFGGV"
     gene            3312..4115
                     /locus_tag="VYN69_000731"
     CDS             3312..4115
                     /locus_tag="VYN69_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEV1533041.1"
                     /translation="MTQDLQKRTLFAGIALAIFLPILMIGGLLLQIAIGIIAMLAMHE
                     LLKMRGLETMTMEGLLTLFATFALTIPLENYLTFLPVDGNVVAYSVLISIMLGTTVFS
                     KSYTIEDAVFPLAMSFYVGFGFNALLDARVAGLDKALLALCIVWATDSGAYLVGMNYG
                     KRKLAPRVSPNKTLEGALGGILGAILVTIIFMIVDSTVALPYGIYKMSVFAIFFSIAG
                     QFGDLLESSIKRHFGVKDSGKFIPGHGGVLDRFDSMLLVFPIMHLFGLF"
     gene            4137..5396
                     /gene="rseP"
                     /locus_tag="VYN69_000732"
     CDS             4137..5396
                     /gene="rseP"
                     /locus_tag="VYN69_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEV1533042.1"
                     /translation="MLGILTFILVFGIIVVVHEFGHFYFAKKSGILVREFAIGMGPKI
                     FAHIGKDGTAYTIRILPLGGYVRMAGWGDDTTEIKTGTPVSLTLADDGKVKRINLSGK
                     KLDQTALPMQVTQFDFEDKLFIKGLVLEEEKTFAVDHDATVVEADGTEVRIAPLDVQY
                     QNATIWGKLITNFAGPMNNFILGVVVFWVLIFMQGGVRDVDTNQFHIMPQGALAKVGV
                     PETAQITKIGSHEVSNWESLIQAVETETKDKTAPTLDVTISEKGSDKQVTVTPEDSQG
                     RYLLGVQPGVKSDFLSMFVGGFTTAADSALRILSALKNLIFQPDLNKLGGPVAIFKAS
                     SDAAKNGIENILYFLAMISINIGIFNLIPIPALDGGKIVLNILEAIRRKPLKQEIETY
                     VTLAGVVIMVVLMIAVTWNDIMRLFFR"
     gene            5409..7262
                     /locus_tag="VYN69_000733"
     CDS             5409..7262
                     /locus_tag="VYN69_000733"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEV1533043.1"
                     /translation="MKQSKMPIPTLREMPSDAQVISHALMLRAGYVRQVSAGVYSYLP
                     LANRVIEKAKNIMRQEFEKIGAVEMLAPALLSAELWRESGRYETYGEDLYKLKNREKS
                     DFILGPTHEETFTAIVRDSVKSYKQLPLNLYQIQPKYRDEKRPRNGLLRTREFIMKDA
                     YSFHANYDSLDSVYDEYKAAYERIFTRSGLDFKAIIGDGGAMGGKDSQEFMAITSART
                     DLDRWVVLDKSVASFDEIPAEVQEEIKAELLKWIVSGEDTIAYSSESSYAANLEMATN
                     EYKPSNRVVAEEEVTRVATPDVKSIDEVAAFLNVPEEQTIKTLFYIADGELVAALLVG
                     NDQLNEVKLKNHLGADFFDVASEEEVANVVQAGFGSLGPVGLPENIKIIADRKVQDVR
                     NAVVGANEDGYHLTGVNPGRDFTAEYVDIREVREGEISPDGQGVLNFARGIEIGHIFK
                     LGTRYSASMGADVLDENGRAVPIIMGCYGIGVSRLLSAVMEQHARLFVNKTPKGEYRY
                     AWGINFPKELAPFDVHLITVNVKDEEAQALTEKLEASLMGAGYEVLTDDRNERVGVKF
                     SDSDLIGLPIRITVGKKAADGIVEVKIKATGDTIEVHADNVLETLEILSKK"
     gene            7361..8740
                     /gene="bglA"
                     /locus_tag="VYN69_000734"
     CDS             7361..8740
                     /gene="bglA"
                     /locus_tag="VYN69_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000943526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1533044.1"
                     /translation="MLRFPKDFVWGSSTSGPQTEGRVAGDGKGDNLWDYWFQVEPNRY
                     YNGIGSDKTSTFYENWERDIELLLETGHTAFRTSIQWSRIFPQGCGKVNTQGVDFYRK
                     VFEAIKAKGIRLLVNLYHFDLPFALQEDGDGWENKATVSAYEDYARFCFETYGDLVDQ
                     WITFNEPIVPVEFGYFYDAHYPHKVDAEAAVKVAYHTQLASSRAVKACHELLPDSKIG
                     IVLNLTPAYPRSQHPADVKAARIAALFQAQSFLDPSVLGTYPQELVEILHEHGLLPDA
                     TEEELELIRDNTVDFLGVNYYQPLRVMAPRFAKHPESPLLPEHFYEPYVMPGRKINSH
                     RGWEIYEQGIYDIAQNIKENYGNIEWMLTENGMGVEGEEKFRQDGMIQDDYRIDFVKG
                     HLRELHRAIEDGANCKGYLIWTFIDCWSWLNSYKNRYGLVELDLETQERRLKKSGHWF
                     KKLSDNNGF"
     gene            8932..10740
                     /gene="glmS"
                     /locus_tag="VYN69_000735"
     CDS             8932..10740
                     /gene="glmS"
                     /locus_tag="VYN69_000735"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014621492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEV1533045.1"
                     /translation="MCGIVGVVGNTNATDILIQGLEKLEYRGYDSAGIFVLDGADNHL
                     VKAVGRIAELSAKTAGVEGTTGIGHTRWATHGKPTEDNAHPHRSETERFVLVHNGVIE
                     NYLEIKEEYLAGHHFKGQTDTEIAVHLIGKFAEEEGLSVLEAFKKALHIIRGSYAFAL
                     VDSQDPEVIYVAKNKSPLLIGLGEGYNMVCSDAMAMIRETNQYMEIHDQELVIVKADS
                     VEVQDYDGNRRERASYTAELDLSDIGKGTYPYYMLKEIDEQPTVMRKLIQAYTDEAGQ
                     VVVDPDIIKAVQDADRIYILAAGTSYHAGFASKKMLEELTDTPVELGISSEWGYGMPL
                     LSKKPLFIFISQSGETADSRQVLVKANEMGIPSLTVTNVPGSTLSREANYTMLLHAGP
                     EIAVASTKAYTAQIAALAFLAKAVGEANGNAKAQVFDLVHELSIVAQSIESTLSEKET
                     IEVKVRELLETTRNAFYIGRGQDYYVAMEASLKLKEISYIQCEGFAAGELKHGTIALI
                     EEGTPVLALLSDPVLANHTRGNIQEVAARGAKVLTIAEENVAKDTDDIVLTTVHPYLS
                     PISMVVPTQLVAYFATLHRGLDVDKPRNLAKSVTVE"
     gene            complement(10877..10954)
                     /locus_tag="VYN69_000736"
                     /pseudo
     CDS             complement(10877..10954)
                     /locus_tag="VYN69_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            11011..12060
                     /locus_tag="VYN69_000737"
     CDS             11011..12060
                     /locus_tag="VYN69_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HEV1533046.1"
                     /translation="MVELGISTFGEITELEGTGQTYSHAERIRQLVAEIELADKVDLD
                     VYGIGEHHRADFAVSAPEIVLAAGAVNTKKIRLTSAVSILSSMDPIRLFQQYATIDAL
                     SNGRAEIMAGRGSFTESFPLFGYDLKDYDSLFDEKLDLLQLVNEKTKLDWQGRLTQTI
                     AGKEVYPRPVQDKLPLWIATGGHVESTVKIAQAGLPIVYAIIGGNPRYFKKLIQAYRE
                     IGSEAGHADKDLKVGAHSWGWIAEDGEQAVKDYFHPTKQVVDAISKDRPHWQELRYEQ
                     YLEQVGSNGAMFVGNPDQVAEKLIRMIEDLGLDRFMLHLPLGSMPHDQVLRAIELFGT
                     QVAPKVRAYFAMKEA"
     gene            complement(12160..15957)
                     /gene="spuA"
                     /locus_tag="VYN69_000738"
     CDS             complement(12160..15957)
                     /gene="spuA"
                     /locus_tag="VYN69_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored pullulanase SpuA"
                     /protein_id="HEV1533047.1"
                     /translation="MRKTPSHTEKKMVYSIRSLKNGTGSVLIGASLVLLAMATPTISS
                     DESTPTTNEPNNRNTTTLAQPLTDTAAGSGKNESDISSPRNANASLEKTEEKPATEPT
                     TSTSPVTTETKAEEPIEDNYFRIHVKKLPEENKDAQGLWTWDDVEKPSENWPNGALSF
                     KDAKKDDYGYYLDVKLKGEQAKKISFLINNTAGKNLTGDKSVEKLVPKMNEAWLDQDY
                     KVFSYEPQPAGTVRVNYYRTDGNYDKKSLWYWGDVKNPSSAQWPDGTDFTATGKYGRY
                     IDIPLNEAAREFGFLLLDESKQGDDVKIRKENYKFTDLKNHSQIFLKDDDESIYTNPY
                     YVHDIRMTGAQHVGTSSIESSFSTLVGAKKEDILKHSNITNHLGNKVTITDVAIDEAG
                     KKVTYSGDFSDTKHPYTVSYNSDQFTTKTSWRLKDETYSYDGKLGADLKEEGKQVDLT
                     LWSPSADKVSVVVYDKNDPDKVVGTVALEKGERGTWKQTLDSTNKLGITDFTGYYYQY
                     QIERQGKTVLALDPYAKSLAAWNSDDAKIDDAHKVAKAAFVDPAKLGPQDLTYGKIHN
                     FKTREDAVIYEAHVRDFTSDPAIAKDLTKPFGTFEAFIEKLDYLKDLGVTHTQLLPVL
                     SYYFVNELKNHERLSDYASSNSNYNWGYDPQNYFSLTGMYSSDPKNPEKRIAEFKNLI
                     NEIHKRGMGAILDVVYNHTAKVDIFEDLEPNYYHFMDADGTPRTSFGGGRLGTTHHMT
                     KRLLVDSIKYLVDTYKVDGFRFDMMGDHDAASIEEAYKAARALNPNLIMLGEGWRTYA
                     GDENMPTKAADQDWMKHTDTVAVFSDDIRNNLKSGYPNEGQPAFITGGKRDVNTIFKN
                     LIAQPTNFEADSPGDVIQYIAAHDNLTLFDIIAQSIKKDPSKAENYAEIHRRLRLGNL
                     MVLTAQGTPFIHSGQEYGRTKQFRDPAYKTPVAEDKVPNKSHLLRDKDGNPFDYPYFI
                     HDSYDSSDAVNKFDWTKATDGKAYPENVKSRDYMKGLIALRQSTDAFRLKSLQDIKDR
                     VHLITVPGQNGVEKEDVVIGYQITAPNGDIYAVFVNADEKAREFNLGTAFAHLRNAEV
                     LADENQAGSVGIANPKGLEWTEKGLKLNALTATVLRVSQNGTSHESTAEEKPDSTPSK
                     PEHQNEASLPAHQDPAPEARPDSTKPDAKVADVENKPSQATADSQAEQPAQEAQASSV
                     KEAVRNESVENSSKENIPATPDKQAELPNTGIKNENKLLFAGISLLALLGLGFLLKNK
                     KEN"
     gene            16374..16787
                     /gene="rpsL"
                     /locus_tag="VYN69_000739"
     CDS             16374..16787
                     /gene="rpsL"
                     /locus_tag="VYN69_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEV1533048.1"
                     /translation="MPTINQLVRKPRKSKVEKSKSPALNVGYNSHKKVQTNVSSPQKR
                     GVATRVGTMTPKKPNSALRKFARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQGRSKYGTKRPKA"
     gene            16807..17277
                     /gene="rpsG"
                     /locus_tag="VYN69_000740"
     CDS             16807..17277
                     /gene="rpsG"
                     /locus_tag="VYN69_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011285383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEV1533049.1"
                     /translation="MSRKNRAPKRDVLPDPLYNSQLVTRLINRVMLDGKRGTAASIVY
                     GAFEQIKEATGNDALEVFETAMENIMPVLEVRARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVTIARLRGEHTMQDRLAKEILDAANNTGAAVKKREDTHRMAEANRAFAHFRW"
     gene            17702..19783
                     /gene="fusA"
                     /locus_tag="VYN69_000741"
     CDS             17702..19783
                     /gene="fusA"
                     /locus_tag="VYN69_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEV1533050.1"
                     /translation="MAREFSLEKTRNIGIMAHVDAGKTTTTERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWNNHRVNIIDTPGHVDFTIEVQRSLRVLDGA
                     VTVLDSQSGVEPQTETVWRQATEYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGSEDDFRGIIDLIKMKAEIYTNDLGTDILEEDIPAEYLDQAQEYREKLIEAV
                     AETDEELMMKYLEGEEITNEELKAGIRKATINVEFFPVLCGSAFKNKGVQLMLDAVID
                     YLPSPLDIPAIKGINPDTDAEETRPASDEEPFAALAFKIMTDPFVGRLTFFRVYSGVL
                     QSGSYVLNTSKGKRERIGRILQMHANSRQEIDTVYSGDIAAAVGLKDTTTGDSLTDEK
                     AKIILESINVPEPVIQLMVEPKSKADQDKMGIALQKLAEEDPTFRVETNVETGETVIS
                     GMGELHLDVLVDRMRREFKVEANVGAPQVSYRETFRASTQARGFFKRQSGGKGQFGDV
                     WIEFTPNEEGKGFEFENAIVGGVVPREFIPAVEKGLVESMANGVLAGYPMVDVKAKLY
                     DGSYHDVDSSETAFKIAASLSLKEAAKSAQPAILEPMMLVTITVPEENLGDVMGHVTA
                     RRGRVDGMEAHGNSQIVRAYVPLAEMFGYATVLRSASQGRGTFMMVFDHYEDVPKSVQ
                     EEIIKKNKGED"
     gene            19887..24278
                     /locus_tag="VYN69_000742"
     CDS             19887..24278
                     /locus_tag="VYN69_000742"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEV1533051.1"
                     /translation="MSNSFEILMNQLGMPAEMRQAPALAQANIERVVVHKISKVWEFH
                     FVFSNILPIEIFLELKKGLSEEFSKTGNKAVFEIKARSQEFSNQLLQSYYREAFSEGP
                     CASQGFKSLYQNLQVRAEGNQLFIEGSEAIDKEHFKKNHLPNLAKQLEKFGFPTFNCQ
                     VEKNDVLTQEQEEAFHAENEQIVQTANEEALRAMEQLEQMAPPPAEEKPVFDFQAKKA
                     AAKPKLDKAEITPMIEVTTEENRLVFEGVVFDVEQKVTRTGRVLINFKMTDYTSSFSM
                     QKWVKNEEEAQKFDLIKKNSWLRVRGNVEMNNFTRDLTMNVQDLQEVVHYERKDLMPE
                     GERRVEFHAHTNMSTMDALPEVEEIVATAAKWGHKAVAITDHGNVQSFPHGYKAAKKA
                     GIQLIYGMEANIVEDRVPIVYNEVEMDLSEATYVVFDVETTGLSAIYNDLIQVAASKM
                     YKGNVIAEFDEFINPGHPLSAFTTELTGITDDHVKNAKPLEQVLQEFQEFCKDTVLVA
                     HNATFDVGFMNANYERHDLPKISQPVIDTLEFARNLYPEYKRHGLGPLTKRFGVALEH
                     HHMANYDAEATGRLLFIFIKEVAEKHGVTDLDRLNIDLISPDSYKKARIKHATIYVKN
                     QVGLKNIFKLVSLSNTKYFEGVPRIPRTVLDAHREGLILGSACSEGEVFDVVVSQGVD
                     AAVEVAKYYDFIEVMPPAIYAPLIAKEQVKDMEELQTIIKSLIEVGDRLGKPVLATGN
                     VHYIEPEEEIYREIIVRSLGQGAMINRTIGHGEHAQPAPLPKAHFRTTNEMLDEFAFL
                     GEELARKLVIENTNALAEIFEPVEVVKGDLYTPFIDKAEETVAELTYKKAFEIYGNPL
                     PDIVDLRIEKELTSILGNGFAVIYLASQMLVQRSNERGYLVGSRGSVGSSFVATMIGI
                     TEVNPLSPHYVCGQCQYSEFITDGSYGSGFDMPHKDCPNCGHKLSKNGQDIPFETFLG
                     FDGDKVPDIDLNFSGEDQPSAHLDVRDIFGEEYAFRAGTVGTVAAKTAYGFVKGYERD
                     YGKFYRDAEVERLAQGAAGVKRTTGQHPGGIVVIPNYMDVYDFTPVQYPADDVTAEWQ
                     TTHFNFHDIDENVLKLDVLGHDDPTMIRKLQDLSGIDPNKIPMDDEGVMALFSGTDVL
                     GVTPEQIGTPTGMLGIPEFGTNFVRGMVDETHPTTFAELLQLSGLSHGTDVWLGNAQD
                     LIKQGIADLSTVIGCRDDIMVYLMHAGLEPKMAFTIMERVRKGLWLKISEEERNGYIE
                     AMKANKVPEWYIESCGKIKYMFPKAHAAAYVMMALRVAYFKVHHPIYYYCAYFSIRAK
                     AFDIKTMGAGLEAIKRRMEEISEKRKNNEASNVEIDLYTTLEIVNEMWERGFKFGKLD
                     LYCSQATEFLIDGDTLIPPFVAMDGLGENVAKQLVRAREEGEFLSKTELRKRGGLSST
                     LVEKMDEMGILGNMPEDNQLSLFDELF"
     gene            24380..24643
                     /locus_tag="VYN69_000743"
     CDS             24380..24643
                     /locus_tag="VYN69_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEV1533052.1"
                     /translation="MSKMSISIRLDSEVKEQAQQVFSNLGMDMTTAINIFLRQAIQYQ
                     GLPFDVRLDENRKLLQALTDLDQNRNMSQSFESVSDLMEDLRA"
     gene            24636..24846
                     /locus_tag="VYN69_000744"
                     /pseudo
     CDS             24636..24846
                     /locus_tag="VYN69_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013904578.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system YafQ family
                     toxin"
     gene            24867..25019
                     /locus_tag="VYN69_000745"
     CDS             24867..25019
                     /locus_tag="VYN69_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533053.1"
                     /translation="MKLRIFAEDKPAKKVFEYQLELADRTILLSTALLSGAIALAGIF
                     SALKEK"
     gene            25041..26282
                     /locus_tag="VYN69_000746"
     CDS             25041..26282
                     /locus_tag="VYN69_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000243957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEV1533054.1"
                     /translation="MVLPNFKENLEKYAKLLVANGINVQPGHTLALSIDVEQRELAHL
                     IVKEAYALGAHEVIVQWTDDVINREKFLHAPMERLDNVPEYKIAEMNYLLENKASRLG
                     VRSSDPGALNGVDADKLSASAKAMGLAMKPMRIATQSNKVSWTVAAAAGLEWAKKVFP
                     NAASDEEAVDFLWDQIFKTCRVYEADPVKAWEEHAAILKSKADMLNKEQFSALHYTAP
                     GTDLTLGLPKNHVWESAGAVNAQGEGFLPNMPTEEVFTAPDFRRADGYVTSTKPLSYN
                     GNIIEGIKVTFKDGQIVDITAEKGDQVMKDLVFENAGARALGECALVPDPSPISQSGI
                     TFFNTLFDENASNHLAIGAAYATSVVDGAEMSEEELEAAGLNRSDVHVDFMIGSNQMD
                     IDGIREDGTRVPLFRNGNWAN"
     gene            26292..26522
                     /locus_tag="VYN69_000747"
     CDS             26292..26522
                     /locus_tag="VYN69_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000901571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1533055.1"
                     /translation="MLGSMFVGLLVGFLAGAMTNRGERMGCFGKMFLGWIGAFLGHLL
                     FGTWGPVLSGTAIIPAVLGAMIVLAIFWRRGS"
     gene            26543..26617
                     /locus_tag="VYN69_000748"
                     /pseudo
     CDS             26543..26617
                     /locus_tag="VYN69_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            26790..27512
                     /locus_tag="VYN69_000749"
     CDS             26790..27512
                     /locus_tag="VYN69_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1533056.1"
                     /translation="MRLDKFLVACAVGSRTEVKNLLKAGRVTVNGKKEKSAKLQIDEK
                     IDEIRFDGQVLEYEEFVYYMMNKPKGVISATEDPKHRTVLDLLDDLARSKEVFPVGRL
                     DIDTHGLLLLTNDGQLAHVLLSPKRHVDKTYLAQVKGIMTQEDVETFAEGIPLKDFTC
                     QPAILELVSIDTEKNQSQIRVTIAEGKFHQIKRMVGYCGKEVVDLQRLTMGTLVLDEN
                     LERGEWRRLTKEELEILRANII"
BASE COUNT         7738 a         5244 c         6507 g         8029 t
ORIGIN      
        1 cctaaacttt tctttttcat aataatctcc cttaactcca cccaatcagg tggagttttt
       61 tagctctatt tcaggctttt ggggactatt ctaaaaataa tttttcgata tttttcggta
      121 tttttcggat tttggtcggg gaattggcgg ggactttttt agcgaatata actaagaaat
      181 aggtctgtgg tcgcttcagc tagttcaaac tcaatttgat tgtaagtgac tgttaattga
      241 agaactggaa ctactgtaat aatgattttg cttgtctagt agtggcgtat taaatgagaa
      301 gtgacatgta agattgtctc ttcgtttacg aaatcaaaat ttctatgaaa ttgatagaat
      361 ccctaacgtt ctcttaatct taaccgtctt gtcgtacagc ggttgaagaa aatgtaaacc
      421 tttgacaaaa ttcgtgaact atgggataat aaaaaggaat tgagtactag ttctatatca
      481 taggctagaa aagaccccaa gctcacgacc aaataagctt gaggtctttt ttgacactat
      541 ttgtccttgt ttagccattt atcgactata cgaagaacga caccgaccac aattggtccg
      601 atgatagttt tgagtactaa ttccatcatg ggctatctca cctcctttcg taggcggtgt
      661 agcagtgtcg tagaatatta taccacataa gtgctaaact taggagtcac ccaatcaggt
      721 ggcttttttg tttgtggctt ggttttttga tataatagag ccatgagtag aattttagat
      781 aatgagataa tgggggatga ggagttagta gaacgcacgc tccgtcctca gtatttacgt
      841 gaatatatcg gacaggataa ggtcaaggac cagctacaaa tctttattga agctgccaaa
      901 atgcgggatg aagcgctgga tcatgtgctc ttatttgggc ctccaggtct cgggaaaacg
      961 accatggcct ttgttattgc caacgaactg ggagtcaatc ttaagcagac gtcgggtcca
     1021 gtcattgaaa aagccggaga tctggtagct attttgaatg agttagagcc tggggatgta
     1081 ctttttattg atgagatcca tcgtttgcca atgtcagtgg aagaggtgct ttatagtgct
     1141 atggaggact tctacatcga tattatgatt ggggctggtg agggtagtcg tagtgttcat
     1201 ttggagttac caccttttac cttgattggt gcgacgactc gggctggtat gctctccaat
     1261 ccgctacggg cacgttttgg gattacaggc catatggagt attatgccca tgctgacttg
     1321 acagaaattg tcgagcggac ggcagatatt tttgagatgg aaatcactca tgaggcagca
     1381 tctgagttgg ccctacgtag tcgtgggacc cctcgtattg ccaatcgtct cctcaagcgc
     1441 gtgcgcgatt ttgcccagat aatggggaat ggggtaattg atgatattat taccgataag
     1501 gctttgacta tgctggatgt tgaccatgaa ggtttggact atgtggatca aaaaatcctt
     1561 cgtaccatga ttgagatgta cagtggagga cctgttggtc taggaactct ttctgtgaat
     1621 atcgccgaag agcgtgagac agttgaagac atgtatgagc cttacttgat tcaaaaaggt
     1681 tttatcatgc ggacacggtc tggacgggtg gcgactgcta aggcatatga gcacttaggt
     1741 tatgaataca gtgaaaaata agcaagaaat cttagaggct tttagagaaa atccagatat
     1801 gatggccatt ctgacgatca tccgagacct tggtctgaaa gactcgtggt tggcagcagg
     1861 ttctgtcaga aatttcattt ggaatctttt gtcagacaaa tccccttttg atcatgaaac
     1921 agatatagat gtgattttct ttgatccaga tttttcttat gaggaaacct tattactgaa
     1981 gaaaaagctg agagaggatt ttcctcagta ccagtgggaa ttgaaaaatc aggtctatat
     2041 gcatcagcac agtcctcaca ctgcttccta taccagttct cgtgatgcta tgagtaagta
     2101 tccagaacgg tgtacggcag ttggactgcg cttgaatgaa gaatcagatt ttgaactcta
     2161 tgtaccttat ggtttggagg atattttgaa ttttcaagtt cgtccaactc ctcatttctt
     2221 agaaaatgaa gaccgaatgg aactctatca aacacgttta tccaagaaaa attggcagga
     2281 gaaatggaaa aatttgattt ttaaaaatac ttaaggaaac tttaagctag ggagtgtaca
     2341 ctaagttcat aagttaagaa gaccttaact taaactccta aaactttttc ataataatct
     2401 ccctataaaa ataaagtcgc ccaatcaggc ggcttatttt tttgacaaat gggcttggtg
     2461 cctgagaata aatagcttag tgatagaaga aaatggggaa atatggtata atgaaacgat
     2521 agatttttga ataggaataa gatcatgttt ggatttttta agaaagataa ggctgtggaa
     2581 gtagaggttc cgacacaggt tcctgctcat atcggcatca tcatggatgg caatggccgt
     2641 tgggctaaaa aacgtatgca accgcgagtt tttggacata aggcgggcat ggaagcattg
     2701 caaaccgtga ccaaggcagc caacaaactg ggcgtcaagg ttattacggt ctatgctttt
     2761 tctacggaaa actggacccg tccagatcag gaagtcaagt ttatcatgaa cttgccagta
     2821 gagttttatg ataattatgt cccggaacta catgcgaata atgttaagat tcaaatgatt
     2881 ggggagacag accgcctgcc taagcaaacc tttgaagctt taaccaaggc tgaggaattg
     2941 actaagaaca acacaggatt gattcttaat tttgctctta actatggtgg acgtgctgag
     3001 attacacagg cgcttaagtt gatttcccag gatgttttag atgccaaaat caatccaggt
     3061 gacatcacag aggaattgat tggtaactat ctctttaccc agcatttgcc taaggactta
     3121 cgagacccag acttgattat ccgtactagt ggagaattgc gtttgagcaa tttccttcca
     3181 tggcagggag cctatagtga gctttatttt acggacacct tatggcctga ttttgacgaa
     3241 gcggccttgc aggaagctat tcttgcctat aatcgtcgtc atcgccgatt tggaggagtt
     3301 taggaggaaa tatgacccag gatttacaga aaagaacctt gtttgcaggg attgccctgg
     3361 ctattttcct accaatttta atgattgggg gcctcttgct tcagatagca atcggaatca
     3421 tagccatgct agccatgcat gaacttttga agatgagagg tctagagacc atgacgatgg
     3481 agggcctctt gaccctcttt gcaacctttg cattgaccat tcccttggag aattacctga
     3541 cttttttgcc agttgatggg aatgtggttg cctatagtgt tttgatttca atcatgttag
     3601 gaacgaccgt ttttagcaag tcttatacga ttgaggatgc ggttttccct cttgctatga
     3661 gcttctacgt gggctttgga tttaatgctt tactagatgc tcgtgttgca ggtttggaca
     3721 aggctctctt agccttgtgt atcgtctggg cgacagacag tggtgcctat cttgttggga
     3781 tgaactatgg gaaacgaaag ttagcaccaa gggtatcgcc taataaaacc cttgagggtg
     3841 ccttgggtgg tattttagga gcaattttag taaccattat ctttatgata gttgacagta
     3901 cagttgctct tccatatgga atttacaaga tgtcagtctt tgctattttc tttagcattg
     3961 ctggacaatt tggtgattta ctagaaagtt cgatcaaacg tcattttggt gttaaggatt
     4021 ctgggaaatt tatccctgga catggtggtg ttttggatcg tttcgatagt atgttgcttg
     4081 tatttccaat catgcactta tttggactct tttaatcaaa agacggagga aacgctatgc
     4141 tcggaatttt aacctttatt ctggtttttg ggattattgt agtggtgcac gagttcgggc
     4201 acttctactt tgccaagaaa tcagggattt tagtacgtga atttgccatc ggtatgggac
     4261 ctaaaatctt tgctcacatt ggcaaggatg gaacggccta taccattcga atcttgcctc
     4321 tgggtggcta tgtccgcatg gccggttggg gtgatgatac aactgaaatc aagacaggaa
     4381 cgcctgttag tttgacactt gctgatgatg gtaaggttaa acgcatcaat ctctcaggta
     4441 aaaaattgga tcaaacagcc ctccctatgc aggtgaccca gtttgatttt gaagacaagc
     4501 tctttatcaa aggattggtt ctggaagaag aaaaaacatt tgcagtggat cacgatgcaa
     4561 cggttgtgga agcagatggt actgaggttc ggattgcacc tttagatgtt caatatcaaa
     4621 atgcgactat ctggggcaaa ctgattacca attttgcagg tcctatgaac aattttatct
     4681 taggtgtcgt tgttttttgg gttttaatct ttatgcaggg tggtgtcaga gatgttgata
     4741 ccaatcagtt ccatatcatg ccccaaggtg ccttggccaa ggtaggagta ccagaaacgg
     4801 cacaaattac caagattggc tcacatgagg ttagcaactg ggaaagcttg atccaagctg
     4861 tggaaacaga aaccaaagat aagacggcac cgactttgga tgtgactatt tctgaaaagg
     4921 ggagtgacaa acaagtcact gttacacccg aagatagtca aggtcgttac cttctaggtg
     4981 ttcaaccggg ggttaagtca gattttctat ccatgtttgt aggtggtttt acaactgctg
     5041 ctgactcagc tctccgaatt ctctcagctc tgaaaaatct gattttccaa ccggatttga
     5101 acaagttggg tggacctgtt gctatcttta aggcaagtag tgatgctgct aaaaatggaa
     5161 ttgagaatat cttgtacttc ttggcaatga tttccatcaa tattgggatt tttaatctta
     5221 ttccgattcc agccttggat ggtggtaaga ttgtgctcaa tatcctagaa gccatccgcc
     5281 gcaaaccatt gaaacaagaa attgaaacct atgtcacctt ggccggagtg gtcatcatgg
     5341 ttgtcttgat gattgctgtg acttggaatg acattatgcg actctttttt agataatcga
     5401 ggaatattat gaaacaaagt aaaatgccta tcccaacgct tcgcgaaatg ccaagcgatg
     5461 ctcaagttat cagccatgct cttatgttgc gtgctggtta tgttcgccaa gtttcagcag
     5521 gtgtttattc ttatctacca cttgccaacc gtgtgattga aaaagctaaa aacatcatgc
     5581 gccaagaatt cgaaaagatt ggtgctgttg agatgttggc tcctgccctt cttagtgcag
     5641 aattgtggcg tgaatcaggt cgttacgaaa cctatggtga agacctttac aaactaaaaa
     5701 accgtgaaaa atcagacttt atcttaggtc caactcatga agaaaccttt acagctattg
     5761 tccgtgattc tgttaaatct tacaagcaat tgccactcaa cctttatcaa attcagccca
     5821 agtatcgtga tgaaaaacgc ccacgtaatg gacttcttcg tacacgtgag tttatcatga
     5881 aggatgctta tagtttccac gctaactatg atagtttgga tagtgtttat gatgagtaca
     5941 aagcagccta tgagcgtatt ttcactcgta gtggtttaga cttcaaggct attattggtg
     6001 acggtggagc catgggtggt aaggatagcc aagaatttat ggccattaca tctgctcgta
     6061 cagaccttga ccgctgggtt gtcttggaca agtcagttgc ctcatttgac gaaattcctg
     6121 cagaagtgca agaagaaatc aaggcagaat tgctcaaatg gatagtctct ggtgaagata
     6181 ccattgctta ctcaagtgag tctagctatg cagctaactt agaaatggca acaaacgagt
     6241 acaaaccaag caaccgtgtt gtcgctgaag aagaagttac tcgtgttgca acgccagatg
     6301 ttaaatcaat tgatgaagtt gcagccttcc tcaatgttcc agaagaacaa acgattaaaa
     6361 ccctcttcta cattgcagat ggcgagcttg ttgcagccct tctagttgga aatgaccaac
     6421 tcaacgaagt caagttgaaa aatcacttgg gagcagattt ctttgacgtt gctagcgaag
     6481 aagaagtggc gaatgttgtt caagcaggat ttggttcact tggaccagtt ggtttgccag
     6541 agaatattaa aattattgca gatcgtaagg tacaagatgt tcgcaatgca gttgtcggtg
     6601 ctaacgaaga tggctaccac ttgactggtg tgaacccagg ccgtgatttt actgcagaat
     6661 atgtggatat ccgtgaagtt cgtgagggtg aaatttcccc agatggacaa ggtgtcctta
     6721 actttgcgcg tggtattgag atcggtcata ttttcaaact cggaactcgc tattcagcaa
     6781 gcatgggagc agatgtcttg gatgaaaatg gtcgtgctgt gccaatcatc atgggatgtt
     6841 acggtatcgg tgtcagccgt cttctttcag cagtgatgga gcaacacgct cgcctctttg
     6901 ttaacaaaac gccaaaaggt gaataccgtt acgcttgggg aatcaatttc cctaaagaat
     6961 tggcaccatt tgatgtgcat ttgattactg tcaatgtcaa ggatgaagaa gcgcaagcct
     7021 tgacagaaaa acttgaagca agcttgatgg gagctggtta tgaagtcttg acagatgacc
     7081 gtaacgaacg tgtcggtgtt aaatttagcg atagtgactt gattggtctt ccaatccgta
     7141 tcactgttgg taaaaaagcg gccgatggca ttgtagaagt taagatcaag gcaactggtg
     7201 acaccattga ggttcatgca gataacgtgc ttgaaacgct tgaaatccta agcaagaaat
     7261 aaaaactata atcagaagaa aaacaaggaa aaaatgtaac tagtttttac cttgtttttt
     7321 ctgtataatg ggagaaatga gtagataaag aggtaaatgt atgctaagat ttccaaagga
     7381 ttttgtctgg ggatcctcta cttctggacc gcagacagaa ggacgtgtag ctggtgacgg
     7441 taagggagac aatctctggg attactggtt ccaagtggag ccaaatcgtt actataatgg
     7501 gattggttca gataagacat cgacttttta tgaaaattgg gagcgggata ttgagctttt
     7561 gttagagact ggtcacacag cctttcggac ttctattcag tggtcacgga tttttccaca
     7621 aggctgtgga aaagtcaaca ctcaaggtgt ggatttttat cgtaaggtct ttgaggctat
     7681 taaggctaaa ggaattcgtc tgttagtcaa tctctatcat tttgatttac cttttgccct
     7741 tcaagaggat ggtgatggtt gggaaaataa ggcgacagtc tcagcctatg aagactatgc
     7801 tcgtttttgt tttgagactt atggagattt agtggatcaa tggattacct ttaacgagcc
     7861 catcgttcct gtagaatttg gttattttta tgatgcccat tatccacata aggtggatgc
     7921 agaggcagct gttaaggtag cctatcatac acaattggcc agcagccggg ctgttaaggc
     7981 ttgccatgaa cttttgcctg attccaagat tgggattgtc ctcaacttga caccggctta
     8041 tccacgtagc cagcatcctg ctgatgtcaa ggcagctcgt attgcggccc tttttcaggc
     8101 ccaatctttc ttagatccat ctgtcttggg gacttatcca caggagttgg tagaaatctt
     8161 gcatgaacac ggtcttttac ctgatgctac agaggaagag ttggaactca ttcgtgataa
     8221 tacggtggac ttccttggtg tgaactacta tcaacctttg cgtgttatgg ctcctcgatt
     8281 tgctaagcat ccagagagtc cactcttacc agaacatttt tacgagcctt atgtgatgcc
     8341 tggacgtaaa atcaattctc accgtggttg ggagatttat gagcaaggga tttatgacat
     8401 cgcccaaaat atcaaggaaa attatggcaa tattgaatgg atgttgactg agaatggtat
     8461 gggtgttgaa ggggaagaaa aattccgtca agatggaatg attcaagatg attaccgtat
     8521 tgactttgta aaaggtcatc ttcgtgaact tcaccgtgcc attgaagatg gtgccaactg
     8581 taagggctac ttgatttgga cctttattga ctgctggtca tggctcaaca gctataaaaa
     8641 tcgctatggt ttggtcgaat tagacttgga aacgcaagaa cgtcgtctga agaaatcagg
     8701 ccactggttc aaaaaattaa gcgataataa tggattttaa aaaggactct gactgattat
     8761 cccaatcggt cagagttttt tgcttacagg agcttaattt gaactatacc aatttttact
     8821 cttgacaagt gaaagaaaca agtatatact gtttttgctg attctataaa agatgaatta
     8881 gactatacca attttaagga gaaagcacag cttgcctgtg tcgtatatac tatgtgtgga
     8941 attgttggtg ttgttggaaa cacaaatgca actgatattt tgattcaagg gcttgaaaag
     9001 cttgaatacc gtggctatga ttctgcggga atttttgtcc tagatggtgc tgataaccat
     9061 ttggtgaagg ctgttggtcg tattgcagaa ttgtctgcca agacagctgg tgttgaggga
     9121 acaactggta tcggacatac tcgttgggct actcacggaa aaccaactga ggacaatgct
     9181 cacccacacc gctctgagac agaacgtttt gtcttggtgc ataatggggt gattgagaac
     9241 taccttgaaa tcaaggaaga ataccttgca ggtcaccact tcaaggggca gacagatact
     9301 gaaatagccg ttcatttgat tggaaaattt gcggaagaag aagggctctc agttcttgaa
     9361 gccttcaaaa aagcccttca catcatccgt ggttcttatg cctttgcctt ggttgactca
     9421 caagatcctg aagtcatcta cgtagctaaa aataaatcac cacttttgat tggtcttgga
     9481 gaaggctata acatggtctg ctcagacgcc atggctatga ttcgtgaaac taaccaatac
     9541 atggaaatcc atgaccaaga gttggtaatc gtcaaggctg atagtgtgga agttcaagac
     9601 tatgatggaa atcgtcgtga gcgtgctagc tacactgctg aacttgactt gtcagatatc
     9661 ggtaagggaa cttaccctta ctacatgctc aaggaaattg acgagcaacc aactgttatg
     9721 cggaaactca tccaagccta cacagatgag gctggtcaag ttgtcgtaga cccagatatc
     9781 attaaggctg ttcaagacgc agaccgcatc tacatccttg cagctggaac atcttaccat
     9841 gcaggatttg cttctaagaa aatgttggaa gaattgacag atacaccagt tgaacttgga
     9901 atctcatctg agtggggcta cggtatgcca cttctcagca agaaaccact cttcatcttt
     9961 atcagccaat ctggtgaaac agcggatagt cgtcaagttt tggtcaaggc taatgaaatg
    10021 ggaattccaa gcttaacagt gacaaatgtt ccaggttcaa ccctctcacg tgaagccaac
    10081 tataccatgc tccttcatgc aggtcctgaa attgctgtgg catcaactaa agcctataca
    10141 gcgcaaatcg cagcccttgc cttccttgca aaagcagtcg gagaagcaaa tggtaatgct
    10201 aaagcgcaag tctttgatct ggttcatgaa ttgtcaatcg tagctcagtc tatcgaatca
    10261 actctttcag agaaagaaac cattgaagtc aaggttcgtg aacttcttga aacaactcgt
    10321 aacgcctttt acatcggacg tggtcaagat tactacgtag ccatggaagc aagtctcaaa
    10381 ctcaaagaga tttcttatat ccagtgtgaa ggctttgctg caggagaact caagcacgga
    10441 accattgcct tgattgaaga aggaacgcct gttttggctc tcttgtcaga tccagttctt
    10501 gccaaccata ctcgtggaaa tatccaagag gtcgcagccc gtggtgccaa agtcctcact
    10561 atcgcagaag agaatgtagc caaagatacc gacgatatcg tccttacgac cgtacatcca
    10621 tacctctcac caatttcaat ggtcgtacca acgcaattag tcgcttactt tgcaaccctc
    10681 catcgtggcc tcgatgtgga caaaccacgt aaccttgcca agtcagtaac ggtagaataa
    10741 gctaaaaaag tctagttatc taggcttttt cttgtgagca aattggttgc ttgtactcaa
    10801 gattcgtgtt atactcttcg aaaatctctt caaaccacgt caacgtcgcc ttgccgtagg
    10861 tatggttact gacttcgtta gttctatcca caacctcaaa acactgtttt gagcagcctg
    10921 cagctagctt cctagtttgc tctttgattt tcattgagta ttagaataat ttttcaaatt
    10981 gaaggacaga gatagacaag gagaatcaca atggtagaat tgggaatttc aacatttggg
    11041 gaaataacgg agcttgaagg gactggacaa acttacagtc atgctgaacg cattcgccag
    11101 ttggtggcag agattgagct ggctgacaag gttgatttgg atgtatatgg gattggtgag
    11161 caccatcggg cggattttgc agtatcagct ccagagattg ttctggccgc tggggcagtc
    11221 aataccaaga aaatccgttt gaccagtgca gtcagcattc tgtcaagcat ggacccgatt
    11281 cgtttgttcc aacagtatgc cactatcgat gctttgtcaa atggacgagc ggagattatg
    11341 gctggaaggg gctctttcac ggaatctttc cctctgtttg gttatgactt gaaagactac
    11401 gactctcttt ttgatgagaa attagacttg cttcagttag tcaatgaaaa gaccaagtta
    11461 gactggcaag gtcgattgac tcaaacgatt gctggtaaag aagtttatcc tcgtccagtt
    11521 caggacaaat tgcccttgtg gatagctaca ggtggtcatg tcgaatcaac agtgaagatt
    11581 gctcaggctg gtctaccgat tgtatatgct attattggtg gtaatccacg ttattttaaa
    11641 aagttgattc aggcttatcg tgagattgga agcgaagcgg gccatgctga caaagacttg
    11701 aaagtgggag cccattcttg ggggtggatt gcggaagatg gtgagcaggc ggtgaaagat
    11761 tatttccatc cgactaagca agtggtggat gctatttcca aagaccgtcc gcactggcag
    11821 gaattgcgtt atgagcaata tttggagcag gtagggtcaa atggcgccat gtttgttggc
    11881 aatccagatc aggtggccga aaaattgatt cgcatgatag aggatttagg attggaccgc
    11941 ttcatgcttc atctaccgct tggttctatg cctcatgacc aggttctgag agctattgaa
    12001 ctcttcggaa cacaagttgc acccaaagta cgagcttatt ttgctatgaa agaggcttaa
    12061 taaaaaatcc taatcaagtt tattaggaca acaaatattg tacaaaagtt aatcccccga
    12121 gctataatgc ttgggggatt tttctatagg agggctagtt tagttctctt ttttattttt
    12181 tagtaagaaa ccgagaccaa ggagcgcaag gaggctgatt cctgcaaata ggagtttgtt
    12241 ttcgtttttg attcctgtat ttggaagttc agcttgttta tctggggttg caggtatatt
    12301 ttccttgcta gagttttcta ccgattcgtt tcgaaccgct tcttttacag atgatgcttg
    12361 tgcttcttgt gctggttgtt cagcttgtga atcagctgta gcttggctag gtttattttc
    12421 cacatcagct actttggcat ctggtttagt agaatcaggt ctagcttctg gagctgggtc
    12481 ttgatgtgca gggagagaag cttcattttg atgttcaggc ttggaagggg ttgagtctgg
    12541 tttctcttct gcagttgact catggctagt tccattttga gagactcgaa gaacagtagc
    12601 tgtaagggca ttcaatttca agcctttttc agtccattca agtcctttcg ggttggcaat
    12661 tccgactgat cctgcttggt tttcatctgc caaaacttcc gcatttctta gatgtgcaaa
    12721 ggcagttccc aaattaaatt cgcgagcttt ttcatccgca ttgacaaaga ctgcgtagat
    12781 atcgccgttt ggagcagtga tttggtagcc aatcactaca tcctcttttt ccacaccatt
    12841 ttggcctggg acagtgatga ggtggacacg gtctttgata tcttgaagac tcttaagtcg
    12901 gaaggcatct gtagattgac gaagggcaat caaacctttc atatagtcac ggctcttgac
    12961 attttcagga taagctttac catctgtagc cttagtccag tcaaacttgt tgactgcgtc
    13021 gctagaatcg taagaatcat ggatgaagta aggatagtca aatgggttgc cgtccttatc
    13081 acgcaacaag tgagatttgt ttggaacctt atcctctgct actggagtct tgtaggctgg
    13141 gtcacggaat tgtttagtac gtccatattc ctgaccggag tggataaatg gagttccttg
    13201 agctgtcaag accatgagat ttccaagtcg taaacgacgg tggatttcag catagttctc
    13261 agctttgctt gggtcttttt tgatagactg ggcaatgatg tcaaagaggg tcaagttatc
    13321 atgggctgcg atgtattgga tgacatctcc agggctgtca gcttcaaagt tagttggctg
    13381 agcaatgaga tttttaaaga tggtgttgac atcacgcttg ccacctgtga taaaggcagg
    13441 ttgaccttcg tttggataac cagatttgag gttgttacgg atgtcatctg aaaagacagc
    13501 gacagtatcg gtatgtttca tccaatcttg gtcagcagct ttagtaggca tgttttcatc
    13561 accggcatag gttctccaac cttcgcctaa catgattaga tttggattga gggcgcgtgc
    13621 agccttgtaa gcttcttcga tagaagcggc gtcatggtct cccatcatat cgaaacggaa
    13681 tccatccact ttgtaggtat caactaggta tttgatagag tcaactagga gccgtttggt
    13741 catatggtgg gttgttccca agcgtccacc accaaagcta gttcgaggtg tgccatctgc
    13801 atccataaag tggtagtagt ttggctctag gtcttcaaag atatcgactt tggctgtgtg
    13861 gttataaacg acatctagga tagctcccat accacgtttg tggatttcgt tgatgaggtt
    13921 tttaaattct gcgattcgtt tttctggatt cttaggatcg cttgagtaca taccagtcaa
    13981 ggagaagtag ttttgagggt catatcccca gttgtagttg ctgttgcttg aagcatagtc
    14041 agacaagcgt tcatggtttt tcaattcatt gacaaagtag taagacaaga ctggaaggag
    14101 ctgggtatgg gttacaccca agtctttgag atagtctagt ttttcaatga aggcttcaaa
    14161 agtcccaaat ggtttggtca agtcttttgc aatggcagga tctgaagtga agtcacgcac
    14221 atgagcttcg tagataacgg cgtcttcacg agtcttgaaa ttgtgaatct taccataagt
    14281 caagtcttga ggtccgagtt tagctggatc tacaaaggcg gctttagcca ctttatgggc
    14341 atcgtcaatc ttggcatcgt cgctattcca agcagcaaga gatttagcgt aaggatcgag
    14401 tgcaagaaca gttttacctt gacgctcgat ttggtattga taatagtagc cagtgaaatc
    14461 tgtgattccg agtttgtttg tgctgtctag agtttgtttc caagttcctc tttccccttt
    14521 ttcgagagcg acagttccaa ctactttgtc agggtcattc ttgtcgtaga caacaacaga
    14581 aaccttatca gcacttggtg accaaagggt caaatcaact tgttttcctt cttcttttag
    14641 gtcagctccc agtttgccat catagctgta tgtctcatct ttcaggcgcc agcttgtttt
    14701 ggtagtgaat tggtcggaat tgtagctaac agtataagga tgttttgtgt cagagaaatc
    14761 tccgctgtag gtcactttct taccagcttc atcgattgca acatcggtaa tagttacctt
    14821 gtttcctagg tgattagtga tgttggagtg tttgaggata tcttcttttt tagcaccgac
    14881 aagtgttgaa aagctacttt caatgctaga agtgcctacg tgttgggctc ctgtcatacg
    14941 gatatcatgg acatagtatg gatttgtgta aatcgattca tcatcgtctt ttaggaaaat
    15001 ttggctatga tttttcaaat ctgtgaactt ataattttct ttacggattt tcacgtcgtc
    15061 tccttgtttg ctctcatcta gtaataaaaa tccaaattct cttgcggctt cattaagagg
    15121 aatgtcgata tagcggccat atttgcctgt agccgtaaag tctgttccgt caggccattg
    15181 agcgctactt ggatttttta catctcccca gtaccagaga gatttcttgt catagttgcc
    15241 atctgtgcgg tagtagttga cgcgaacagt tcctgcaggc tgtggctcgt aagagaaaac
    15301 cttgtaatct tggtctaacc aagcctcgtt catttttgga actagttttt ctacagattt
    15361 atcgccggtt agattttttc cagctgtatt gttgatgagg aagctaattt tcttggcttg
    15421 ttctcccttt aatttaacat ctaggtaata gccgtagtca tctttcttgg catccttgaa
    15481 ggacaaagct ccgtttggcc agttttcaga tggtttttca acatcgtccc aagtccatag
    15541 tccttgagca tccttgtttt cttcaggaag ttttttgaca tggatacgga agtagttatc
    15601 ttcgatgggc tcttctgcct tagtttcagt tgttactgga ctagtagaag tagttggctc
    15661 tgttgcaggt ttttcttctg ttttctctag ggaagcgttt gcatttctag gtgaagaaat
    15721 atcactttcg ttcttaccag agccagctgc tgtatcagta agaggttggg caagggtggt
    15781 tgtatttctg ttgttgggtt cgttagtggt tggtgtactt tcgtcggatg agatagttgg
    15841 tgtagccatg gcaagcaaga caaggcttgc gccgataagg acagaaccag ttccattttt
    15901 gagggaacgg atgctgtaga ccattttttt ctcagtgtga gatggtgttt ttctcataat
    15961 gattctccaa tcaataaatt tctatatcag tatagcatgt agtaaaacaa tatgcaagcg
    16021 ttttctttga tttgtgaaaa aaattcttac ttttgagaag ttttctgtac tattttatat
    16081 atcagtataa atgacggaga taaaaatgtc acataagcca agccgtatta ttaggaagat
    16141 gatgtgttcc cagctatttt tattcagaaa ttgatctcta acattaccaa taaaaaagaa
    16201 aaatatcttg acaaacaagg gaaaattttg ttacaataat agacggtact ttttactttt
    16261 ggtctctcaa gagtgtacag ggacgtgctg acaaatgttg caaaagtaca cacagatgat
    16321 atctgtcacc aagtgtatca tcaccaaaaa taaaaaaaca caggagaatg tagatgccta
    16381 caattaacca attggttcgc aaaccgcgta aatcaaaagt agaaaaatct aaatcaccag
    16441 ctttgaacgt tggttacaat agtcataaaa aagttcaaac aaacgtttct tcaccacaaa
    16501 aacgtggtgt tgcaactcgt gttggaacaa tgacacctaa aaaacctaac tcagcccttc
    16561 gtaaattcgc tcgtgtacgt ttgagcaacc ttatcgaagt tactgcctac atcccaggta
    16621 tcggacacaa cttgcaagag cacagcgtgg tgcttcttcg cggtggacgt gtaaaagacc
    16681 ttccaggggt acgttaccat atcgtccgtg gtgcacttga tactgcaggt gttaacgatc
    16741 gtaaacaagg ccgttctaaa tacggtacta aacgtccaaa agcataagga aaggggataa
    16801 agagaaatga gtcgtaaaaa tagagctcca aaacgtgacg tattgccaga tccgctatac
    16861 aattcacaac tagttactcg tcttatcaac cgcgttatgc ttgatggtaa acgtggtact
    16921 gctgcttcaa tcgtttacgg tgcttttgag caaatcaaag aagctactgg caacgatgca
    16981 cttgaagtat ttgaaacagc tatggaaaac atcatgcctg tacttgaagt acgtgcacgt
    17041 cgtgttggtg gttctaacta ccaagtccca gttgaagttc gtccagaacg tcgtacaaca
    17101 cttggacttc gttggttggt aacaatcgct cgtcttcgtg gtgaacacac aatgcaagac
    17161 cgtcttgcaa aagaaatctt ggatgctgct aacaacactg gtgcagcagt taagaaacgt
    17221 gaagatactc accgtatggc tgaagctaac cgtgcattcg cacacttccg ttggtaagat
    17281 aggatgcgaa agcgttaaga aagtcccaga gaaaataggg aatcgaagca ggttgcggtt
    17341 gcaaccaatg agattcatct ttttctccag acttttagct tgagctcaac taaatcatga
    17401 tgctaggcac ggtaaggatg caaggtgaaa ataggaaact gacgcagtat tcaacgaata
    17461 caaggagttt tatctttttc acgcagcatc ccgttccagc tcacatcggc taactaactt
    17521 tagcccgggt tcaaattagc taaatcgatt agtattagct ataactcagc ttaccatctc
    17581 gtaagttgaa accaacaata gcatgaaaac attgagaacg ggtaggtcct gcctatccgt
    17641 ttttattaaa atcgtgttat aatagaatag aaatcaaaaa taaataggag aaacaaacct
    17701 catggcacgc gaattttcac ttgaaaaaac tcgtaatatc ggtatcatgg ctcacgtcga
    17761 tgccggtaaa acaacaacta ctgagcgtat tctttactac actggtaaaa tccacaaaat
    17821 cggtgaaact cacgaaggtg cgtcacaaat ggactggatg gagcaagagc aagaacgtgg
    17881 tatcacgatc acatctgctg cgacaacagc tcaatggaac aaccaccgcg taaacatcat
    17941 cgacacacca ggacacgtgg acttcacaat cgaagtacaa cgttctcttc gtgtattgga
    18001 tggtgcggtt accgttcttg actcacaatc aggtgttgaa cctcaaactg aaacagtttg
    18061 gcgtcaagca actgagtacg gagttccacg tatcgtattt gccaacaaaa tggacaaaat
    18121 cggtgctgac ttcctttact ctgtaagcac acttcacgat cgtcttcaag caaatgcaca
    18181 cccaatccaa ttgccaatcg gttctgaaga tgacttccgt ggtatcattg acttgatcaa
    18241 gatgaaagct gaaatctata ctaacgacct tggtacggat atccttgaag aagacatccc
    18301 agctgaatac cttgaccaag ctcaagaata ccgtgaaaaa ttgattgaag cagttgctga
    18361 aactgacgaa gaattgatga tgaaatacct cgaaggtgaa gaaatcacta acgaagaatt
    18421 gaaagctggt atccgtaaag cgactatcaa cgttgaattc ttcccagtat tgtgtggttc
    18481 agccttcaaa aacaaaggtg ttcaattgat gcttgatgcg gttatcgact accttccaag
    18541 tccacttgac atcccagcaa tcaaaggtat taacccagat acagacgctg aagaaactcg
    18601 tccagcatct gacgaagagc catttgcagc tcttgccttc aagatcatga ctgacccatt
    18661 cgtaggtcgt ttgacattct tccgtgttta ctcaggtgtt cttcaatcag gttcatacgt
    18721 attgaatact tctaaaggta aacgtgaacg tatcggacgt atccttcaaa tgcacgctaa
    18781 cagccgtcaa gaaatcgaca ctgtttactc aggtgatatc gctgctgccg ttggtttgaa
    18841 agatactaca actggtgact cattgacaga tgaaaaagct aaaatcatcc ttgagtcaat
    18901 caacgttcca gaaccagtta tccaattgat ggttgagcca aaatctaaag ctgaccaaga
    18961 caagatgggt atcgcccttc aaaaattggc tgaagaagat ccaacattcc gcgttgaaac
    19021 aaacgttgaa actggtgaaa cagttatctc aggtatgggt gaacttcacc ttgacgtcct
    19081 tgttgatcgt atgcgtcgtg agttcaaagt tgaagcgaac gtaggtgctc ctcaagtatc
    19141 ttaccgtgaa acattccgcg cttctaccca agcccgtgga ttcttcaaac gtcagtctgg
    19201 tggtaaaggt caattcggtg atgtgtggat tgaatttact ccaaacgaag aaggtaaagg
    19261 attcgaattc gaaaacgcaa tcgtcggtgg tgtggttcct cgtgaattta tcccagcggt
    19321 tgaaaaaggt ttggtagaat ctatggctaa cggtgttctt gcaggttacc caatggttga
    19381 cgttaaagct aagctttatg atggttcata tcacgatgtc gactcatctg aaactgcctt
    19441 caagattgcg gcttcacttt cccttaaaga agctgctaaa tcagcacaac cagctatcct
    19501 tgaaccaatg atgcttgtaa caatcactgt tccagaagaa aaccttggtg atgttatggg
    19561 tcacgtaact gctcgtcgtg gacgtgtaga tggtatggaa gcacacggta acagccaaat
    19621 cgttcgtgct tacgttccac ttgctgaaat gttcggttac gcaacagttc ttcgttctgc
    19681 atctcaagga cgtggtacat tcatgatggt atttgaccac tacgaagatg tacctaagtc
    19741 agtacaagaa gaaattatta agaaaaataa aggtgaagac taatccgtcc tcactctaga
    19801 aggaagtcac ttagtggctt ccttttgtct ttagaaaata cctctaaata tggtaaaata
    19861 gtagaagaat aatgtgagga aaatgaatgt caaatagttt tgaaattttg atgaatcaat
    19921 tggggatgcc tgctgaaatg agacaggctc ctgctttagc acaggccaat attgagcgag
    19981 ttgtggttca taaaattagt aaggtatggg agtttcattt cgtattttct aatattttac
    20041 cgattgaaat ctttttagaa ttaaagaaag gtttgagcga agaattttct aagacaggca
    20101 ataaagctgt ttttgaaatt aaggctcggt ctcaagaatt ttcaaatcag ctcttgcagt
    20161 cctactatag ggaggctttc tctgaaggtc catgtgctag tcaaggtttt aagtcccttt
    20221 atcaaaattt gcaagttcgt gctgagggca atcagctatt tattgaagga tctgaggcga
    20281 ttgataagga acattttaag aagaatcatc ttcctaattt agccaaacaa cttgaaaagt
    20341 ttggttttcc aacttttaac tgtcaagtcg agaagaatga tgtcctgacc caagagcagg
    20401 aagaggcctt tcatgctgaa aatgagcaga ttgttcaaac tgccaatgag gaagcgctcc
    20461 gtgctatgga acaactggag cagatggcac ctcctccagc ggaagagaaa ccagtctttg
    20521 attttcaagc gaaaaaagct gcagctaaac ccaagctgga taaggcggag attactccta
    20581 tgatcgaagt gacgacagag gaaaatcgtc tggtatttga aggggttgtt tttgatgtgg
    20641 agcaaaaagt gactagaaca ggtcgtgttt taatcaactt taaaatgacg gactatactt
    20701 caagtttttc tatgcaaaag tgggttaaaa acgaggaaga ggcccagaag tttgacctca
    20761 tcaagaagaa ttcttggctc cgagttcgag ggaatgtgga gatgaataac ttcacacgcg
    20821 atttgactat gaacgtacag gatctgcagg aagttgttca ctatgagcgg aaggatttga
    20881 tgccagaagg tgagcgtcgg gttgagtttc atgctcatac taacatgtcg actatggatg
    20941 ctttgccaga ggtcgaagag attgttgcaa cagctgctaa gtggggacac aaggcggttg
    21001 ctatcacgga ccatgggaat gtccagtcct ttccacatgg ctataaggcg gctaagaaag
    21061 cgggaatcca gctgatctat ggaatggaag ccaatatcgt ggaggaccgt gtccctatcg
    21121 tctataacga agtggagatg gacttgtcag aagcaaccta cgtggtcttt gacgtggaaa
    21181 cgacgggact ttcagctatc tataatgact tgattcaggt tgcggcctct aagatgtaca
    21241 aggggaatgt tattgctgaa tttgatgaat ttatcaatcc tgggcatccc ttgtcagctt
    21301 ttactacaga gttaactgga attacagatg atcatgtcaa aaatgccaaa ccactagaac
    21361 aagttttgca agaattccaa gaattttgca aggatacggt cctagttgcc cacaatgcta
    21421 cctttgacgt tggctttatg aatgctaatt atgagcgtca tgatcttcca aagattagtc
    21481 agccagttat tgatacgctg gagtttgcta gaaacctcta tcctgagtat aaacgccatg
    21541 gtttggggcc tttgaccaag cgttttggtg tggccttgga acatcaccac atggccaact
    21601 acgatgcgga agcgactggt cgtctgcttt tcatctttat caaagaggta gcagaaaaac
    21661 atggtgtgac cgatttagat agactcaaca ttgatctaat cagtccagat tcttacaaaa
    21721 aagctcggat caagcatgcg accatctatg tcaagaatca ggtaggtcta aaaaatatct
    21781 ttaagctggt ttccttgtct aataccaagt attttgaagg agtgccacgg attccgagaa
    21841 cggttctaga tgcccatcga gagggcttga ttttaggttc agcctgttca gagggtgaag
    21901 tttttgacgt ggtcgtttct caaggtgtgg atgcggcggt tgaggtggcc aagtattatg
    21961 actttatcga ggtcatgcca ccggctatct atgcgccctt gattgccaaa gagcaggtca
    22021 aggatatgga ggaactccag accattatca agagtttgat agaggttgga gaccgccttg
    22081 gcaagcctgt tctggctacg ggaaatgttc actatatcga accggaagaa gagatttatc
    22141 gtgaaattat cgtccgtagt ttgggacagg gtgcgatgat taaccgaact atcggtcatg
    22201 gtgaacatgc ccaaccagcg ccacttccaa aagctcattt tcgaacgact aatgagatgt
    22261 tggatgaatt tgcctttttg ggagaggaac tggctcgtaa actggttatt gaaaacacca
    22321 atgccttggc agaaatattt gaacccgttg aagtcgttaa gggtgacttg tatacgcctt
    22381 tcatcgacaa ggctgaagaa acagttgctg agttgaccta taagaaagct tttgagattt
    22441 atgggaatcc gctgccagat attgttgatt tgcggattga aaaagaatta acatccatac
    22501 tggggaatgg atttgctgtg atttatttgg catcgcagat gctggtgcaa cgttctaatg
    22561 aacggggtta tttggttggt tctcgtgggt ctgtcggatc tagtttcgtt gcgaccatga
    22621 ttgggattac ggaggtcaat cctctctctc ctcactatgt ctgtggtcag tgtcagtaca
    22681 gtgagtttat cacagatggt tcgtacggtt caggatttga tatgccccat aaggactgtc
    22741 caaactgtgg tcacaaactc agtaaaaacg gacaggatat tccgtttgag accttccttg
    22801 gttttgatgg ggataaggtt cctgatattg acttgaactt ctcgggagaa gatcagccta
    22861 gcgcccactt ggatgtgcgt gatatctttg gtgaagaata tgccttccgt gcgggaacgg
    22921 ttggtacagt agctgccaag actgcctatg gatttgtcaa gggttacgag cgagattatg
    22981 gcaagtttta tcgtgatgca gaagtagaac gcctcgctca aggagcggcg ggtgtcaagc
    23041 ggacaacagg ccaacacccg gggggaatcg ttgttattcc gaactacatg gatgtctacg
    23101 attttacgcc tgtccagtat ccagcagatg atgtcacggc tgaatggcag accactcact
    23161 ttaacttcca cgatatcgat gagaacgtcc tcaaactcga tgtactggga catgatgatc
    23221 cgaccatgat tcgaaaactt caggatttgt ctggtattga ccctaataaa attcctatgg
    23281 atgacgaagg cgtgatggca ctcttttctg ggactgatgt gctaggggta acacctgaac
    23341 aaattggaac gcctacgggt atgttgggga ttccagagtt tggaacaaat ttcgtacgtg
    23401 gaatggtaga cgaaacccat ccgacaacct ttgcggaatt gcttcagctg tctggtctgt
    23461 cccacggtac tgacgtttgg ttggggaatg ctcaggatct gattaagcaa ggaatagcgg
    23521 acctatcgac tgttatcggt tgtcgggacg acatcatggt ttacctcatg catgcgggtc
    23581 tggaacctaa gatggccttt accattatgg aacgggtacg taagggtttg tggctaaaga
    23641 tttcagaaga ggagagaaat ggctatatcg aagccatgaa ggctaataag gtgccagagt
    23701 ggtatatcga atcctgtggg aaaattaagt acatgttccc taaggcccat gcggcagcct
    23761 acgttatgat ggccttgcgt gtagcttact tcaaggttca ccatcctatt tattactact
    23821 gtgcttactt ctccattcgt gctaaggctt ttgatatcaa gaccatgggt gcgggcttgg
    23881 aggccatcaa gcgcagaatg gaagaaatct ctgaaaaacg gaagaacaat gaagcctcta
    23941 atgtggaaat cgatctctat acaactcttg agattgtcaa tgagatgtgg gaacgaggtt
    24001 tcaagtttgg taaattagat ctctactgta gtcaggcgac agagttcctc atcgacgggg
    24061 atacccttat cccaccattt gtagcaatgg atggtctggg agagaacgtt gccaagcaac
    24121 tggtgcgggc gcgtgaagag ggagaattcc tctctaaaac agaactacgc aagcgtggtg
    24181 gactctcatc aaccttggtt gaaaagatgg atgagatggg tattcttgga aatatgccag
    24241 aggataacca gttgagtttg tttgatgagt tgttttaaaa aattgcttaa taatctatta
    24301 aaagaggcta acgtatatcc aatagattta cattagcttt cttttttgtt aaaatagtct
    24361 atggaaagag ggtgagagta tgtcaaagat gagtataagc atccgtctgg atagtgaggt
    24421 taaggagcag gcccaacagg tgtttagtaa tctgggaatg gatatgacaa cagctattaa
    24481 tattttcctt cgtcaggcaa ttcaatatca gggactacct tttgatgtta gactagacga
    24541 aaatcggaag ttgctccaag cgttaacgga tttagaccaa aatcgtaata tgagccagtc
    24601 ttttgaatca gtctcagatt tgatggagga cttacgtgct taagattcgt tatcataaac
    24661 agtttaaaaa agattttaag ttggctatga agcgtggttt gaaggcagaa ttattagaag
    24721 aagttttgaa ttttctggtt caagaaaaag aacatcctgc cagattggct tttggtttat
    24781 aaagtagaca agtcggaatt gattttaaat ttgctgagga caggcagtca cagtgattta
    24841 ttttaatcta ttttaagggg gttctcatga aactaagaat atttgcggaa gataagccgg
    24901 ctaagaaggt atttgaatat caattagaac ttgctgatcg tacaattctt ctatcgacag
    24961 cactcttgtc aggtgctatt gctttagcag gaatcttttc tgctttgaaa gaaaaataaa
    25021 aatagaaaag agaaaacaga atggttttac caaattttaa agaaaatcta gaaaaatatg
    25081 cgaaattgtt ggttgcgaac ggaattaacg tgcaacctgg tcacactttg gctctctcta
    25141 ttgatgtgga gcaacgtgaa ttggcacatc taatcgtgaa agaagcttat gccttgggtg
    25201 cgcatgaggt catcgttcag tggacagatg atgtgattaa ccgtgagaaa ttcctccatg
    25261 ccccgatgga gcgtttggac aatgtgccag aatacaagat tgctgagatg aactatctct
    25321 tggagaataa ggctagccgt cttggagttc gttcatctga tccaggtgcc ttgaacggag
    25381 tggacgctga caagctttca gcttctgcta aagctatggg acttgccatg aagcctatgc
    25441 gtatcgcaac tcaatctaac aaggttagct ggactgtagc agctgcagca ggacttgagt
    25501 gggctaagaa agtcttccca aatgctgcga gcgacgaaga agcagttgat ttcctttggg
    25561 accaaatttt caaaacttgc cgtgtctacg aagcagatcc tgttaaggcc tgggaagagc
    25621 atgcagctat cttgaagagc aaggccgata tgcttaataa ggagcaattt tcagcccttc
    25681 actacacagc gccaggaaca gatttaacac ttggtttgcc aaagaaccat gtttgggaat
    25741 cagctggtgc tgtcaatgca cagggcgaag gattcttgcc aaatatgccg acagaagagg
    25801 tcttcacagc acctgacttc cgtcgtgcag atggttatgt cacttctaca aaaccgctta
    25861 gctacaacgg aaatattatt gaaggcatta aggtgacctt taaggatgga caaatcgtag
    25921 atatcactgc tgagaagggt gatcaggtta tgaaagacct tgtctttgaa aatgcgggtg
    25981 cgcgtgcctt gggtgaatgt gccttggtac cagatccaag tccaatttct cagtcaggca
    26041 ttaccttctt taacaccctt ttcgatgaaa atgcgtcaaa ccacttggct atcggtgcag
    26101 cctatgcgac tagcgttgtt gatggagcgg agatgagcga agaggagctt gaagctgcag
    26161 ggcttaaccg ttcagatgtt cacgtagact ttatgattgg ttctaaccaa atggatatcg
    26221 atggtattcg tgaggatgga acgcgggtac ctcttttccg taatgggaat tgggcaaatt
    26281 aaggagataa tatgttagga agtatgttcg ttggtctcct agtgggattt ttagcaggtg
    26341 ctatgaccaa tcgtggagag cgaatgggat gttttggaaa aatgtttctc ggttggatcg
    26401 gagcctttct aggtcacttg ctctttggaa cttgggggcc agttttatca ggaacagcta
    26461 ttatcccagc tgttttaggt gccatgattg tcttagctat tttttggaga cgaggaagtt
    26521 aatttcctaa atctgatact caatgaaaat caaagagcaa actaggaaac tagccgcagg
    26581 ttgctcaaag cactgctttg aggttgcaga tagaactgac gaagtcagct caaaacactg
    26641 ttttgaggtt gcagatagaa ctgacgaagt cagtaacata tatacggcaa ggcgacgttg
    26701 acgcggtttg aagagatttt tgaagagtat gaaacgaaaa ggaggttggt cattgtacca
    26761 gcctcctttt gatatgatat aatagttcta tgagattaga taaattttta gttgcctgtg
    26821 cggtgggaag tcggactgag gtcaaaaact tgctcaaggc tgggcgcgtg actgtaaatg
    26881 gtaaaaaaga aaaatcagct aaattgcaga ttgatgaaaa aatagatgag attcgctttg
    26941 atgggcaagt gttggagtat gaagagtttg tctactacat gatgaacaag cccaaaggag
    27001 ttatctcagc gactgaggat cccaagcaca gaaccgttct ggacttgttg gatgacttgg
    27061 cgcggagtaa ggaagttttc ccagtaggac gcttggatat tgacacgcat ggtcttttgc
    27121 tcttgaccaa tgatggtcag ctggctcatg ttcttctttc gcccaagcgt catgtggaca
    27181 agacttatct ggcacaagtc aagggaatca tgacccaaga agatgtggag acatttgctg
    27241 agggtattcc tctcaaagac tttacctgtc aacccgctat actggagctt gtatccatag
    27301 atacagaaaa gaatcaaagc caaatccgtg tgaccattgc agaagggaag tttcatcaga
    27361 tcaagcgtat ggtgggctac tgtggcaagg aagtggtgga cttgcaacga ttgactatgg
    27421 gaactttagt attagatgag aacctggaac gaggggaatg gcgtcgcttg accaaggaag
    27481 aattagaaat tctccgcgct aatattattt aatttagt
//
LOCUS       DAVLVP010000018        27342 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.018, whole genome shotgun sequence.
ACCESSION   DAVLVP010000018 DAVLVP010000000
VERSION     DAVLVP010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 27342)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27342)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27342
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.018"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            407..1570
                     /locus_tag="VYN69_000750"
     CDS             407..1570
                     /locus_tag="VYN69_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEV1533057.1"
                     /translation="MNRIRVSKRVEKKLAKGLVLLEASDLENVNLKDQEVEVQGQEGN
                     FLGTAYLSQQNKGLGWFVSKDKVAFNQAFFETLFRKAKEKRSAYYQDDLTTAYRLFNQ
                     EGDGFGGLTVDLYGDYAVFSWYNSYVYQIRQTISEAFRQVFPEVLGAYEKIRFKGLDY
                     ESAHVYGQEAPDFFTVLENGVLYQVFMNDGLMTGIFLDQHEVRGSLVDGLAMGKSLLN
                     MFSYTAAFSVAAAMGGASQTTSVDLAKRSRELSQAHFQANGLSTDEHRFIVMDVFEYF
                     KYAKRKDLTYDVIVLDPPSFARNKKQTFSVAKDYHKLISQSLEILNPGGIIIASTNAA
                     NVSRQKFTEQIDKGFAGRSYQILNKYGLPADFAYNKKDESSNYLKVISMKVSK"
     gene            1567..2244
                     /locus_tag="VYN69_000751"
     CDS             1567..2244
                     /locus_tag="VYN69_000751"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEV1533058.1"
                     /translation="MKLIVSVMPRSLEEAQALDATRYLDADIIEWRADYLPKEAILQV
                     APAIFEKFVGRELVFTLRTRSEGGEIDLSPEEYIHLIKEVAQLYQPDYIDFEYYSYKD
                     VFEEMLDFPNLVLSYHNFQETPENMMEILSELTILNPKLVKVAVMAHTEQDVLDLMNY
                     TRGFKTLNPEQEYVTISMGKVGKVSRITADVTGSSWSFASLDEASAPGQISLASMKKI
                     REILDEA"
     gene            2234..3088
                     /locus_tag="VYN69_000752"
     CDS             2234..3088
                     /locus_tag="VYN69_000752"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEV1533059.1"
                     /translation="MKLDGCTRLAAVVANPIKHSISPFIHNRAFEATDTNGAYVAWEI
                     EASDLVETVANIRRYQMFGINLSMPYKEQVIPYLDKLSDEARLIGAVNTVVNENGNLI
                     GYNTDGKGFFKCLPSFTISGKKMTLLGAGGAAKSILAQAILDGVSQISVFVRSVSMEK
                     TRPYLDKLQEQTGFKVDLCALEYVSELQARIAESDLLVNATSVGMDGQSSPVPENIVL
                     PETLSVADIIYQPFETPFLKWARSQGNPAVNGLGMLLYQAAEAFQLWTGKEMPTEEIW
                     QSLTEKYQ"
     gene            3107..4174
                     /locus_tag="VYN69_000753"
     CDS             3107..4174
                     /locus_tag="VYN69_000753"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEV1533060.1"
                     /translation="MKIRIDIPHHPYDIQIEKGCMAQAGQWLRELWQPQKVVIVTDNH
                     VASLYAEKVKLSLEDAGFQVAVFDFLEGEERKNLTTVQKVYEFLVKQGLTRSDGIVAL
                     GGGVVGDLAGFVASTYMRGIHFVQIPTSLTAQVDSSIGGKTGVNTPFAKNMVGTFAQP
                     DGVLIDPLVLETLGKRELIEGMGEVIKYGLIEDPELWALLTGLNGSVESILEHAETLI
                     EHSCQVKRKMVVEDELDNGIRLYLNFGHTIGHAIEATAGYGKVMHGEAVAMGMVQISK
                     IAEEKGLIPAGITQSITEMCQKFGLPVDYENWEVDKLYQALTHDKKARGNTLKLVLVP
                     ELGSAIIHPVSLEEMKDYLVK"
     gene            4184..5350
                     /gene="aroC"
                     /locus_tag="VYN69_000754"
     CDS             4184..5350
                     /gene="aroC"
                     /locus_tag="VYN69_000754"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEV1533061.1"
                     /translation="MRYLTAGESHGPRLTAIIDGIPAGLPLTAEDINEDLRRRQGGYG
                     RGGRMKIENDQVVFTSGVRHGKTTGAPITMDVINKDHQKWLDIMSAEDIEDRLKSKRK
                     ITHPRPGHADLVGGIKYRFDDLRNSLERSSARETTMRVAVGAVAKRLLAELDMEIANH
                     VVVFGGKEIDVPENLTVAEIKQRAAQSEVSIVNQEREQEIKDYIDQIKRDGDTIGGVV
                     ETVVGGVPVGLGSYVQWDRKLDARLAQAVVSINAFKGVEFGLGFEAGYRKGSQVMDEI
                     LWSKEDGYTRRTNNLGGFEGGMTNGQPIVVRGVMKPIPTLYKPLMSVDIETHEPYKAT
                     VERSDPTALPAAGMVMEAVVATVLAQEILEKFSSDNLEELKEAVAKHRDYTKNY"
     gene            5360..6463
                     /locus_tag="VYN69_000755"
     CDS             5360..6463
                     /locus_tag="VYN69_000755"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEV1533062.1"
                     /translation="MAKTIYIAGLGLIGASMALGIKRDHPDYEILGYNRSQASRDIAL
                     KEGMIDRATDDFASFAPLADVIILSLPIKQTIAFIKELANLDLREGVIISDAGSTKST
                     IVDAAEQYLAGKSVRFVGAHPMAGSHKTGAASADVNLFENAYYIFTPSSLTSQDTLKE
                     MKDLLSGLHARFIEIDAKEHDRVTSQISHFPHILASSLMEQTAVYAQEHEMARRFAAG
                     GFRDMTRIAESEPGMWTSILLSNSEIILDRIEDFKERLEAIGQAISKGDEEQIWNFFN
                     QAREQRQTMEIHKRGGVDSSYDLYVDVPDEEDVILRILELLRGTSLVNIHINEENRED
                     IHGILQISFKNAQDLERAEHLITENTDYTVVIK"
     gene            6474..6812
                     /locus_tag="VYN69_000756"
     CDS             6474..6812
                     /locus_tag="VYN69_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HEV1533063.1"
                     /translation="MSNIYDSANELSRGLRGLPEYKAVKAAKDAIAADAEASKIFTEY
                     LAFQEEIQKLAQTGQMPDASFQAKMEGFGKQIQGNSLLSEFFTKQQQLAIYLSDIEKI
                     VFEPVSELLK"
     gene            6904..8187
                     /gene="aroA"
                     /locus_tag="VYN69_000757"
     CDS             6904..8187
                     /gene="aroA"
                     /locus_tag="VYN69_000757"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004236106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEV1533064.1"
                     /translation="MKLKTNIRHLHGSIRVPGDKSISHRSIIFGSLAEGETKVYDILR
                     GEDVLSTMQVFRDLGVEIEDKDGVITIQGVGMAGLKAPQNALNMGNSGTSIRLISGVL
                     AGADFEVEMFGDDSLSKRPMDRVTLPLKKMGVSISGQTERDLPPLRLKGTKNLRPIHY
                     ELPIASAQVKSALMFAALQAKGESVIIEKECTRNHTEDMLKQFGGHLSVDGKKIIVQG
                     PQKLTGQKVVVPGDISSAAFWLVAGLIVPNSRLVLQNVGINETRTGIIDVIRAMGGKL
                     EITEIDPVAKSATLTVESSDLKGTEIGGALIPRLIDELPIIALLATQAQGVTVIKDAE
                     ELKVKETDRIQVVADALNSMGADITPTADGMIIKGKSALHGARVNTFGDHRIGMMTAI
                     AALLVADGEVELDRAEAINTSYPSFFDDLESLIHG"
     gene            8180..8656
                     /locus_tag="VYN69_000758"
     CDS             8180..8656
                     /locus_tag="VYN69_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEV1533065.1"
                     /translation="MAKVLLGFMGAGKSTIARGLDPNYLDMDALIEKRLGMSIANFFA
                     EKGEAAFRQVESEVLADLLQTDQVVSTGGGVVISQRNRDLLKTNTDNIYLKADFETLY
                     LRIVADKDNQRPLFLNNSKEELVVIFQERQAWYEEVASRVLDVTKLSPEEIIEELR"
     gene            8653..9501
                     /gene="pheA"
                     /locus_tag="VYN69_000759"
     CDS             8653..9501
                     /gene="pheA"
                     /locus_tag="VYN69_000759"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1533066.1"
                     /translation="MKIAYLGPKGSFSHHVVQTAFPHEELQAFANITDVIKAYEQGLV
                     NYSVVPVENSIEGSVHETLDYLFHQAHIQAVAEIVQPIHQQLMVVPGHTKIEKIFSHP
                     QALAQGKKFIDEQYPEAQIEVTASTAYAARFISEHPDQPFAAVAPRSSAEEYGLELIA
                     EDIQEMEANFTRFWVLGAEKPSIPLQAQTEKMSLALTLPDNLPGALYKALSTFAWRGI
                     DLTKIESRPLKTALGEYFFIIDVDYTDKDLVHFAQKELEAIGIQYKILGAYPIYPISD
                     HGKERR"
     gene            9498..10772
                     /gene="psr"
                     /locus_tag="VYN69_000760"
     CDS             9498..10772
                     /gene="psr"
                     /locus_tag="VYN69_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase Psr"
                     /protein_id="HEV1533067.1"
                     /translation="MSKENPLSHHEQLRYDYLLKNIHYLNEREKNEFAYLQEKLTLAR
                     GNSSSSLEQEREEQVDLPSYANRSRSQSKSQALSSPPKKKRRKLRLKRIFMVIFSLLV
                     CVALAMVFMFLRGYQDASAKKTADARAAQVEVFNGQDTRDGVNILIMGTDGRIGQNSV
                     ETRTDSIMVLNVGGSDKKMKLVSFMRDNLVYIDGYSQVINGRKQTDNKLNVAYELGEQ
                     EGQKGAEMVRQVLKDNFDLDIKYYALVDFQAFATAIDTLFPDGVTIDAQFSTLNGRPL
                     TEATVGDDLYATETESPTQTIKVGKQQMNGSTLLNYARFRDDDEADYGRTKRQQQVLT
                     AILEQIKDPTKLFTGSEALGKVFAMTSTNVPYTFLLTNGLSVLDGAKNGIEKLTIPEL
                     GDWVDAYDVYGGLGLLVDQNKYQTKLAQMGLR"
     gene            11051..11896
                     /locus_tag="VYN69_000761"
     CDS             11051..11896
                     /locus_tag="VYN69_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1533068.1"
                     /translation="MNNMTDLKAIQARSLEMAEYFVAFCKEHDLLCYLCGGGAIGALR
                     NKGFIPWDDDLDFFMPRKDYEKLAELWPRYADERYFLSKSHKDFVDRNLFITIRDKET
                     TCIKPYQQDLDLPHGLALDILPLDYYPKNPAERKKQVRWALIYSLFCAQTIPEKHGAL
                     MKWGSRILLGLTPKSLRYRIWKKAEKEMTKYDLADCDGITELCSGPGYMRNKYPITSF
                     EDNLFLPFEGTEMPIPIGYDVYLRTAFGDYMTPPPADKQVPHHDAVIADMDKSYTEYK
                     GEYGG"
     gene            11886..12989
                     /locus_tag="VYN69_000762"
     CDS             11886..12989
                     /locus_tag="VYN69_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000225894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEV1533069.1"
                     /translation="MVAKKKILFFMWSFSLGGGAEKILSTIVSNLDPEKYDIDILEME
                     HFDKGYESVPKHVRILKSLQDYRQTRWLRAFLWRMRIYFPRLARRLLVKDDYDVEVSF
                     TIMNPPLLFSKRREVKKISWIHGSIEELLKDSSKRESHRSQLDAANTIVGISKKTSNS
                     IKEVYPDYTSKLQTIYNGYDFQTILEKSQEKIDIEIAPQSICTIGRIEENKGSDRVVE
                     VIRLLHQEGKNYHLYFIGAGDMEEELKKRVKEYGIEDYVHFLGYQKNPYQYLSQTKVL
                     LSMSKQEGFPGVYVEALSLGLPFISTDVGGAEELSQEGRFGQIIESNQEAAQAITNYM
                     TSASNFDVDEASQFIQQFTITKQIEQVEKLLEE"
     gene            12991..13977
                     /locus_tag="VYN69_000763"
     CDS             12991..13977
                     /locus_tag="VYN69_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000446977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1533070.1"
                     /translation="METALISVIVPVYNVAQYLEKSIASIQKQTYQNLEIILVDDGAT
                     DESGRLCDSIAEQDDRVSVLHKKNEGLSQARNDGMKQAHGDYLIFIDSDDYIHPEMIQ
                     SLYEQLVQEDADVSSCGVMNVYANDESPQSANQDDYFVCDSQTFLKEYLIGEKIPGTI
                     CNKLIKRQIATALSFPKGLIYEDAYYHFDLIKLAKKYVVNTKPYYYYFHRGDSITTKP
                     YAEKDLAYIDIYQKFYNEVVKNYPDLKEVAFFRLAYAHFFILDKMLLDDQYKQFEAYS
                     QIHRFLKGHAFAISRNPIFRKGRRISALALFINISLYRFLLLKNIEKSKKLH"
     gene            13993..15426
                     /locus_tag="VYN69_000764"
     CDS             13993..15426
                     /locus_tag="VYN69_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen polysaccharide polymerase Wzy family
                     protein"
                     /protein_id="HEV1533071.1"
                     /translation="MIDGKRLLFSLTIVSYALTLVSGIVYLFNNNNVSLLSTLLFLLV
                     SSLIACWNDIKYYLIHFIFYLTIFVFLVSRPTIDYFRDGALDTYHPIAYRFAFIVVMI
                     SILGLTTGGILARYFIARKKIKVVNIGNSLKEVYIKRLRFVSLGVFLLTYPFYFIRLF
                     ERLLYRLQTSYYAYYANFESKLPYFTYILSTFTVYAMCMYLATKPKKLQATAVLVSFI
                     AANTIHLAIGTRNPFILSILFAFVYYFMREQTEKGKWIGFKEKLAIFVGSPILMLAMG
                     VLNYVRDNVQVSHTGFWDILLDFIYKQGTSFGVLARGFLFNSSLPYRDFRNFTFGPVL
                     DYFARGSLGAIFGGKAFEHTTNSVELAIDSNSYAHNLSYLVLNKEYLKGHGIGSSYIM
                     ELYTDYGMIGVFLLSFLLGVLFIAMLQVAYRSRTILFALSLLILNNLFFMPRSSFSES
                     FFNLFTMQFWGIVLVIIFVAKMLTKEN"
     gene            15455..16489
                     /locus_tag="VYN69_000765"
     CDS             15455..16489
                     /locus_tag="VYN69_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEV1533072.1"
                     /translation="MFEHYSVADLFANLYKKRKANILALIALFALIAVPFTIKAVRNK
                     NTVKDTTSYSTYLIYKITPPKESAKTILNHQIGGYSDFYGKLIDGNLNGAYLFNDVES
                     SELKKIASELDTTETTLKNSTNDYWWKKLTVYYMIDDAGVGVKILTSSKDANDLLEKK
                     IDGLIEKFKHAYANVKIEKLETINSKELNANGETALGLNVKNLILRLVVIGVVCVILV
                     VMGNVLVYLFNPTINRVGDFSQYQIDFVTEITTIANLADVLSYKNTGQELTIVSSNKA
                     ILDKLKQSQEALKGMHFVDLQDVSSLLERDTVLLVEEYGVTRYKKFEQSLQILRNLNR
                     SILGVATFKL"
     gene            16689..17426
                     /gene="mecA"
                     /locus_tag="VYN69_000766"
     CDS             16689..17426
                     /gene="mecA"
                     /locus_tag="VYN69_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEV1533073.1"
                     /translation="MKMKQISDTTLKITMSLEDLMDRGMEIADFLVPQEKTEEFFYAI
                     LDELEMPDSFLDTGMLSFRVTPKPDKVDVFVTKSKIDQNLDFEDLSDLPDMEELAQMS
                     PDEFIKTLEKSIADKTKDDIEAIQSLEQVEAKEEEQEQAEQEAESKKEPYIYYILSFA
                     KLADLVVFAKTVTFEMETSELYKMNERYYLTILVDIENHPSPYPAWLLARMREFADDS
                     DISRSVLQEYGQVLMSHDAVLNLQKIG"
     gene            17577..18863
                     /locus_tag="VYN69_000767"
     CDS             17577..18863
                     /locus_tag="VYN69_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HEV1533074.1"
                     /translation="MTVKIALLGFGTVASGVPFLLKENGGKINQSAHSDIKVAKVLVK
                     DEDEKNRLLAAGNDFNFVTNVDDILSDQDITIVVELMGRIEPAKTFITRALEAGKHVV
                     TANKDLLAVHGAELLEIAQANKVALYYEAAVAGGIPILRTLANSLASDKITRVLGVVN
                     GTSNFMVTKMVEEGWSYDDALAEAQRLGFAESDPTNDVDGIDAAYKMVILSQFAFGMK
                     IAFDDVAHKGIRNITPEDVAVAQELGYVVKLVGSIEETSSGIAAEVTPTFLPKAHPLA
                     SVNGVMNAVFVESIGIGESMYYGPGAGQKPTATSVVADIVRIVRRLNDGTIGKDFNEY
                     SRDLVLANPEDVKANYYFSILALDSKGQVLKLAEIFNAQDISFKQILQDGKEGNKARV
                     VIITHKINKAQLENVSAELKKVSEFDLLNTFKVLGE"
     gene            18865..19734
                     /locus_tag="VYN69_000768"
     CDS             18865..19734
                     /locus_tag="VYN69_000768"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HEV1533075.1"
                     /translation="MKIIVPATSANIGPGFDSVGVAVTKYLQIEVCEERDEWLIEHQI
                     GKWIPHDERNLLLKIALQIVPDLQPRRLKMTSDVPLARGLGSSSSVIVAGIELANQLG
                     QLNLSDHEKLQLATKIEGHPDNVAPAIYGNLVIASSVEGQVSAIVADFPECDFLAYIP
                     NYELRTRDSRSVLPKKLSYKEAVAASSIANVAVAALLAGDMVTAGQAIEGDLFHERYR
                     QDLVREFAMIKQVTKENGAYATYLSGAGPTVMVLASHDKMPTIKAELEKQPFKGKLHD
                     LRVDTQGVRVEAK"
     gene            19810..19884
                     /locus_tag="VYN69_000769"
                     /pseudo
     CDS             19810..19884
                     /locus_tag="VYN69_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829418.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            20029..20967
                     /gene="msrB"
                     /locus_tag="VYN69_000770"
     CDS             20029..20967
                     /gene="msrB"
                     /locus_tag="VYN69_000770"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1533076.1"
                     /translation="MAEIYLAGGCFWGLEEYFSRISGVLETSVGYANGQVETTNYQLL
                     KETDHAETVQVIYDEKEVSLREILLYYFRVIDPLSINQQGNDRGRQYRTGIYYQDEAD
                     LPAIYTVVQEQERMLGRKIAVEVEQLRHYILAEDYHQDYLRKNPSGYCHIDVTDADKP
                     LIDAANYEKPSQEVLKASLSEESYRVTQEAATEAPFTNAYDQTFEEGIYVDITTGEPL
                     FFAKDKFASGCGWPSFSRPISKELIHYYKDLSHGMERIEVRSRSGSAHLGHVFTDGPR
                     ELGGLRYCINSASLRFVAKDEMEKAGYGYLLPYLNK"
     gene            21025..22749
                     /locus_tag="VYN69_000771"
     CDS             21025..22749
                     /locus_tag="VYN69_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533077.1"
                     /translation="MKHLLSYFKPYIKESILAPLFKLLEAVFELLVPMVIAGIVDQSL
                     PQGDQGHLWMQIGLLLIFAVIGVLVALIAQFYSAKAAVGFAKELTNDLYRHILSLPKD
                     SRDRLTTSSLVTRLTSDTYQIQTGINQFLRLFLRAPIIVFGAIFMAYRISAELTFWFL
                     VMVAILTIVIVGLSRLVNPLYSSLRKKTDQLVQETRQQLQGMRVIRAFGQEKRELQIF
                     QTLNQVYARLQEKTGFWSSLLTPLTYLIVNGTLLVIIWQGYISIQGGVLSQGALIALI
                     NYLLQILVELVKLAMLINSLNQSYISAKRIEEVFVEAPEDIHSELEQKQATRDKVLQV
                     QELTFTYPDAAQPSLRDISFDMTQGQILGIIGGTGSGKSSLVQLLLGLYPVDKGNIDL
                     YQNGRSPLNLEQWRSWIAYVPQKVELFKGTIRSNLTLGFNQEVSDQKLWQALEIAQAK
                     DFVSEKEGLLDALVEAGGRNFSGGQKQRLSIARAVLRQAPFLILDDATSALDTITESK
                     LLKAIRENFPNTSLILISQRTSTLQMADQILLLEKGELLAVGKHDDLMKSSQVYREIN
                     ASQHGKED"
     gene            22751..24499
                     /locus_tag="VYN69_000772"
     CDS             22751..24499
                     /locus_tag="VYN69_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533078.1"
                     /translation="MKRQTVNQTLKRLAVDLANHPFLLFLAFLGTIAQVGLSIYLPIL
                     IGQVIDQVLVAGSSPVFWQIFLQMLLVVIGNTLVQWANPLLYNRLIFSYTRDLRERII
                     HKLHRLPIAFADRQGNGEMVSRVTTDIEQLAAGLTMIFNQFFIGVLMILVSILAMLQI
                     HLLMTLLVLLLTPLSMVISRFIAKRSYHLFQKQTETRGIQTQLIEESLSQQTIIQSFN
                     AQTEFIQRLREAHDNYSGYSQSAIFYSSTVNPSTRFVNALIYALLAGVGAYRIMMGST
                     LTVGRLVTFLNYVQQYTKPFNDISSVLAELQSALACVERIYGVLDSPEVAETGKEVLT
                     SDQVKGAISFKHVSFGYHPEKILIKDLSIDIPAGSKVAIVGPTGAGKSTLINLLMRFY
                     PISSGDILLDGQSIYDYTRVSLRQQFGMVLQETWLTQGTIHDNIAFGNPEASREQVIA
                     AAKAANADFFIQQLPQGYDTKLENAGESLSVGQAQLLTIARVFLAIPKILILDEATSS
                     IDTRTEVLVQDAFAKLMKGRTSFIIAHRLSTIQDADLILVLVDGDIVEYGNHQELMDR
                     KGKYYQMQKAAAFSSE"
     gene            24756..26015
                     /locus_tag="VYN69_000773"
     CDS             24756..26015
                     /locus_tag="VYN69_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRZ/ATZ family protein"
                     /protein_id="HEV1533079.1"
                     /translation="MKVFQHVNIVTCDQDFHVYLDGILAVKDSQIVYVGQDKPAFLEQ
                     AEQIIDYQGAWIMPGLVNCHTHSAMTGLRGIRDDSNLHEWLNDYIWPAESEFTPDMTT
                     NAVKEALTEMLQSGTTTFNDMYNPNGVDIQQIYQVVKTSKMRCYFSPTLFSSETETTA
                     ETISRTRSIIDEILKYKNPNFKVMVAPHSPYSCSRDLLEASLEMAKELNIPLHIHVAE
                     TKEESGIILKRYGKRPLAFLEELGYLDHPSVFAHGVELNEREIERLASSQVAIAHNPI
                     SNLKLASGIAPIIQLQKAGVAVGIATDSVASNNNLDMFEEGRTAALLQKMKSGDASQF
                     PIETALKVLTIEGAKVLGMENQIGSLEVGKQADFLVIQPQGKIHLQPQENMLSHLVYA
                     VKSSDVDDVYIAGEQVVKQGQVLTVEL"
     misc_feature    26091..26226
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            26265..26765
                     /locus_tag="VYN69_000774"
     CDS             26265..26765
                     /locus_tag="VYN69_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001287276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEV1533080.1"
                     /translation="MSEAIIAKKAELVDVVAEKMKAAASIVVVDARGLTVEQDTVLRR
                     ELRGSEVEYKVIKNSILRRAAEKAGLEDLASVFVGPSAVAFSNEDVIAPAKILNDFSK
                     NAEALEIKGGAIEGAVASKEEILALATLPNREGLLSMLLSVLQAPVRNVALAVKAVAE
                     SKEDAA"
     gene            26841..27209
                     /gene="rplL"
                     /locus_tag="VYN69_000775"
     CDS             26841..27209
                     /gene="rplL"
                     /locus_tag="VYN69_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEV1533081.1"
                     /translation="MALNIENIIAEIKEASILELNDLVKAIEEEFGVTAAAPVAVAAA
                     DAADAGAAKDSFDVELTSAGDKKVGVIKVVREITGLGLKEAKELVDGAPALVKEGVAT
                     AEAEEIKAKLEEAGASVTLK"
BASE COUNT         8086 a         5113 c         6169 g         7974 t
ORIGIN      
        1 aattattgtt gcttctataa tattatttat tgatagtata ttgagtttgt aacaaaaggc
       61 ttttctataa aatcaaattt tccagatacc tactgaagct gatttatttc ttcatctttc
      121 ttctactacc tttgtcacta aatttgacag acacatattg ctgatacaac ctctctacct
      181 ataatctcac agttaacctg aggctagttt catagtttgc tatttgattt tcgttgataa
      241 aaagaaatgc tcctccaaga cggaagagca ttttttattg tgattcttac acatttactt
      301 cctagttttg caggaagtct agtataccat tttttaaaag aagagtaaaa aagcaagggt
      361 agtttctttt ttgagaattg tctaaaagtt tgatataatg gtagttatga atagaataag
      421 agtcagcaaa agggttgaaa agaagcttgc taaggggcta gttttactag aagccagtga
      481 tcttgagaat gtcaatctta aggatcagga agtagaggtg cagggtcagg aaggaaactt
      541 tcttgggact gcctaccttt ctcagcaaaa caagggcttg ggctggtttg tcagcaaaga
      601 caaggtggcc ttcaatcaag ctttctttga aacgttgttt agaaaagcca aagaaaagag
      661 aagcgcctac tatcaagacg atttaacaac tgcctatcgt ctctttaatc aagagggaga
      721 tggctttggg ggtctgacag tggaccttta tggcgactac gccgtctttt cttggtataa
      781 ctcttatgtt tatcagattc gtcagactat atcagaagcc tttagacagg ttttccctga
      841 ggttttaggg gcttatgaga aaatccgctt taagggtttg gactatgaat ctgcccatgt
      901 ttatggtcaa gaagcacctg actttttcac tgttttagaa aatggtgtcc tgtatcaagt
      961 ctttatgaat gatggcttga tgacaggaat tttcctagac cagcatgagg ttcgcggtag
     1021 tttagttgac ggcttggcta tgggtaaatc cttgctcaat atgttttcct acacagcggc
     1081 tttttcagta gctgcggcca tgggaggagc tagccagaca acttctgttg atctagccaa
     1141 acgttcacga gaattgtctc aagcgcattt tcaggcaaat gggctcagca cagacgagca
     1201 tcgttttata gtcatggatg tctttgagta tttcaaatat gccaaacgca aagacttgac
     1261 ctacgatgtg attgtcctag atccgcctag ctttgctcgg aataaaaaac aaactttctc
     1321 tgtggccaag gattatcaca agttgatttc ccagagtctt gagattttaa atccgggagg
     1381 gattatcatt gccagtacca atgctgccaa tgtttcccgt cagaaattta cagaacaaat
     1441 tgataaaggc tttgcaggaa gaagttacca gattttaaac aaatatggtc ttccagcaga
     1501 ttttgcctat aataaaaaag atgaaagtag taattacctc aaggtgatta gtatgaaggt
     1561 tagtaaatga aattaatcgt ttcagtaatg ccaagaagtt tagaggaggc tcaggctctg
     1621 gatgccacga ggtacctgga tgccgacatc attgaatggc gtgccgacta tctgcctaaa
     1681 gaagcgattt tgcaggtagc tccagccatt tttgaaaaat tcgtaggccg tgagttggtt
     1741 ttcacgctac gaactcgttc cgaaggtgga gaaatcgacc tttctccaga agagtatatc
     1801 catctaatca aggaagttgc gcaactctat caaccagact atattgattt tgagtactat
     1861 agctacaagg atgtttttga ggaaatgctg gacttcccaa atcttgtttt gagttaccat
     1921 aatttccaag aaacacctga gaacatgatg gaaatcttgt cagagttgac gatcctaaat
     1981 ccaaaacttg ttaaggttgc ggtgatggct cacacggagc aggatgtctt agacttgatg
     2041 aactatacac gaggctttaa aaccctcaat cctgaacagg aatatgtgac catttctatg
     2101 ggtaaggtgg gcaaggtctc tcgtatcact gcggatgtga ctggttcgag ttggtctttt
     2161 gccagtctgg atgaggctag tgcccctggg cagatttctc tagctagcat gaaaaaaatc
     2221 agggagattt tggatgaagc ttgatggctg tacacgttta gctgcagttg ttgccaatcc
     2281 tattaagcat tctatttctc ccttcatcca caatagagcc tttgaggcga cagataccaa
     2341 cggtgcttat gtggcttggg agattgaagc gagtgacttg gtagaaacag tggccaatat
     2401 tcgtcgctac cagatgtttg gcatcaatct gtccatgccc tataaggagc aggtgattcc
     2461 ttatttggat aagctgagcg atgaagcgcg cttgattggt gcggttaata cggttgtcaa
     2521 tgagaatggc aatttaattg gatataatac agatggcaag ggatttttta agtgcttgcc
     2581 ttcttttaca atttcaggta aaaagatgac cctgctgggt gcaggtggtg cggctaaatc
     2641 aatcttggca caggctattt tggatggcgt cagtcagatt tcggtctttg ttcgttccgt
     2701 ttctatggaa aaaacaagac cttacctaga caagttacag gagcagacag gctttaaagt
     2761 ggatttgtgt gctttagaat atgtttctga actgcaagca aggattgccg agtcggattt
     2821 gctagttaat gccaccagtg tgggcatgga tggccaatcc tctcctgttc ctgaaaacat
     2881 agtcttacca gaaactctct cagttgcaga tatcatatac caaccctttg aaacaccgtt
     2941 tttgaaatgg gctagaagtc agggcaatcc agcagtcaat ggtctgggaa tgttactcta
     3001 tcaagctgca gaagcttttc aactgtggac aggcaaggaa atgccgacag aagagatttg
     3061 gcagtcttta acagaaaaat accaataaag aaagggagac tctcctatga aaatcagaat
     3121 cgatattcct catcatcctt atgatattca gattgaaaaa ggttgtatgg cccaggctgg
     3181 tcagtggttg cgagaactct ggcaaccgca aaaggtagtc attgtgacag ataaccatgt
     3241 agcctctctc tatgcagaga aggtcaagct cagcctagaa gatgctggtt ttcaggtagc
     3301 tgtttttgat ttcttagaag gtgaagaaag aaagaattta actactgttc agaaagtcta
     3361 tgaatttcta gtcaagcaag gtctgactcg tagcgatgga atcgttgctc ttggtggtgg
     3421 cgttgttggg gacctggctg gctttgtagc ctctacctat atgcggggta ttcactttgt
     3481 tcagattccg actagtttga cagctcaggt tgattcttct atcggtggaa agacaggcgt
     3541 taacacacca tttgctaaaa atatggtggg gacctttgcc caaccagatg gggttctgat
     3601 tgatccactt gttcttgaaa ccctcggaaa aagagagttg attgaaggga tgggtgaagt
     3661 tatcaagtat ggcttgattg aggatccaga actgtgggct ctcttgacgg gactgaatgg
     3721 ttctgttgag agcattttgg aacatgcaga gaccttgatt gaacattctt gtcaggtgaa
     3781 gcgcaagatg gtggttgaag atgagttgga caatggtatt cgtctttacc tcaattttgg
     3841 ccacactatt ggccatgcca tcgaagcgac tgccggttat ggcaaggtca tgcatggaga
     3901 ggctgttgcc atgggaatgg tacagatttc caagattgct gaggaaaaag gcctcatacc
     3961 agctggcatt acccaatcta tcacagagat gtgtcagaaa ttcggcttgc ctgttgacta
     4021 tgaaaattgg gaagttgaca agctttatca ggctcttact catgacaaga aagcgcgtgg
     4081 taacaccttg aaattggtct tggtgccaga gcttggttca gcgatcattc acccagtttc
     4141 tctggaagag atgaaagact acttggtaaa ataaggagag tgtatgagat atttaactgc
     4201 aggagaatca cacggccccc gtctaacagc tattattgat ggaattccag ctggacttcc
     4261 attgacagct gaggatatca atgaggacct tagacgccgt cagggtggct acggtcgtgg
     4321 tggtcgtatg aagattgaga atgaccaggt tgtctttact tcgggcgttc gccacgggaa
     4381 gacgacaggg gcgcctatta ctatggatgt catcaataag gaccaccaga aatggctgga
     4441 catcatgtct gcggaggaca ttgaagaccg ccttaaaagc aagcggaaaa ttactcatcc
     4501 tcgcccaggt catgccgatt tggttggggg gattaagtac cgttttgatg atttgcgaaa
     4561 ttctttggag cgttcatcag ctcgtgaaac caccatgcgg gtggcagttg gtgcagtagc
     4621 caaacgcctc ttggctgagc tggatatgga gattgccaac catgtcgtgg tctttggtgg
     4681 taaggaaata gatgttcctg aaaatctgac agtcgctgaa attaagcaaa gagctgccca
     4741 gtctgaagtt tctattgtca accaagaacg agaacaggaa atcaaggact atattgacca
     4801 aatcaaacgt gatggtgata ccatcggtgg ggttgtggag acagtcgtcg gaggcgttcc
     4861 agttggtctt ggttcctatg tccaatggga tagaaaattg gatgcaagat tggctcaagc
     4921 tgttgtctct atcaatgcct ttaaaggggt ggaatttggt cttggctttg aggctggtta
     4981 tcgtaaaggc agccaagtta tggatgaaat tctctggtct aaagaagacg gttatactcg
     5041 ccgtaccaat aatctaggtg gttttgaagg tggtatgact aatgggcaac ccatcgttgt
     5101 tcgtggggtc atgaaaccca ttcctactct ttataaacct cttatgagtg tggatatcga
     5161 aacccacgaa ccttacaagg caaccgtgga gagaagtgat ccgactgctc ttccagctgc
     5221 aggaatggtc atggaagcag ttgtagcaac ggttctggcg caagaaatcc tcgaaaaatt
     5281 ctcatcagat aatcttgagg aactaaaaga agcggtagcc aaacaccgag actatacaaa
     5341 gaactattaa ggagttccta tggcaaaaac aatctatatc gcaggtcttg ggttgattgg
     5401 agcctctatg gcacttggta tcaaacgcga tcatccagat tatgaaattt taggttataa
     5461 tcgtagtcaa gcttcgagag atatcgcctt gaaagaaggc atgattgacc gtgcaacgga
     5521 tgattttgct agttttgctc ctttggcaga tgtcattatc ctcagcttgc caatcaaaca
     5581 aactattgct ttcattaagg agttggccaa tttggatttg cgagaaggcg ttattatttc
     5641 agatgctggt tcgaccaagt caaccattgt ggatgcggcg gagcagtatt tggctggcaa
     5701 gtctgttcgc tttgtcgggg cccatcccat ggctggtagt cacaagacag gggctgcttc
     5761 ggcagatgtc aatctttttg aaaatgccta ttatatcttt acaccttcaa gcctgacaag
     5821 tcaggacacg cttaaggaaa tgaaggatct actttcaggt cttcatgctc gttttatcga
     5881 gattgatgcc aaggagcatg atcgtgtcac ttctcagatt agccattttc ctcatatttt
     5941 ggcttctagt ctcatggagc agactgcggt ctatgctcaa gagcatgaga tggcaaggcg
     6001 ctttgcggca ggtggttttc gagatatgac ccgaattgcg gaaagcgagc caggaatgtg
     6061 gacctccatt ctcttgtcca atagcgagat cattctggat agaattgagg atttcaagga
     6121 acgtttggaa gcgattggtc aggccattag taagggagat gaagagcaaa tttggaactt
     6181 ttttaaccaa gcgcgtgaac aacgccagac catggaaatc cataagcgtg gtggtgtgga
     6241 tagctcttac gacctctatg ttgacgttcc tgatgaagaa gatgtcatcc tgcggatttt
     6301 ggaactgcta cgtggaactt ccttggttaa catccacatc aacgaggaaa atcgtgagga
     6361 tattcacgga atcctacaaa tttcatttaa aaatgctcaa gacttggaaa gagctgagca
     6421 tctcataaca gaaaataccg actatacagt cgtcatcaaa taaggagaag aacatgtcaa
     6481 atatttacga tagtgcaaac gaactcagtc gcggtctacg cggattacca gaatacaagg
     6541 ctgttaaagc agctaaagat gcgattgcag cagatgctga ggcaagcaaa atctttacag
     6601 aatatcttgc ctttcaagag gaaattcaaa aactagcaca gacaggtcaa atgccagatg
     6661 cgtccttcca agctaagatg gaaggctttg gtaaacagat tcaaggaaat agcctcttgt
     6721 cagaattctt taccaagcaa caacaattgg ccatttacct ttctgacatt gaaaaaattg
     6781 tttttgaacc agtttcagaa ttgctgaaat aagaaaagaa cgatcctaaa gtcaggaatt
     6841 tttaaggaat ttctggcttt ttatgataaa ataggtaaaa gtattgcaag tatgaggtcc
     6901 agtatgaaac taaaaacaaa cattcgccat ttacatggta gtatccgcgt cccaggtgac
     6961 aagtctatca gccaccgttc cattatcttt ggaagtttgg ctgagggtga gaccaaggtt
     7021 tatgatattc tgcgaggcga agacgttctt tcgaccatgc aggtttttcg tgaccttggt
     7081 gttgaaattg aggataaaga tggggttatt accattcaag gtgtaggcat ggctggctta
     7141 aaagcgccgc aaaatgccct taatatggga aattctggca cctcgattcg cctgatttca
     7201 ggtgtccttg ctggtgcaga tttcgaagta gagatgtttg gagatgatag tctttccaaa
     7261 cgtcctatgg accgtgtgac ccttccactg aaaaaaatgg gcgtcagcat ttcagggcaa
     7321 actgaacgag acttgcctcc ccttcgctta aaagggacga aaaacctaag acctattcat
     7381 tatgagttgc caattgcctc tgcccaagtc aagtcagcct tgatgtttgc agccttacaa
     7441 gctaaggggg agtcagttat tatcgaaaaa gagtgcactc gtaatcatac tgaagatatg
     7501 ttgaaacaat ttggtggtca tttaagtgtg gacggtaaga aaatcatagt ccaagggcca
     7561 caaaaattga caggacagaa ggtggtcgta ccaggagata tttccagtgc agccttttgg
     7621 ttagtcgcag gtttgattgt tccaaattct cgtctagtgc tgcagaatgt ggggatcaac
     7681 gaaactcgca ccggtattat tgatgtcatt cgtgccatgg gtggaaaatt ggaaataact
     7741 gaaatcgatc cagtcgctaa atctgcaacc ttgactgttg agtcttctga cttgaaagga
     7801 acagagattg gtggcgcttt gattccacga ttgattgatg aattgcccat tattgccctg
     7861 cttgcgactc aagcccaagg tgtaacagtt atcaaggatg ctgaggagct caaggtcaag
     7921 gaaacagacc gtattcaggt tgtggcagac gccttaaata gtatgggagc agatattact
     7981 cctacggcag atgggatgat tatcaaaggg aaatcagctc ttcacggtgc tagagtcaat
     8041 acgtttggtg accaccgtat cggcatgatg acagctatcg cagccctctt ggttgcagat
     8101 ggagaggtgg agcttgaccg tgcagaagcc atcaatacta gctatcctag tttctttgat
     8161 gatttggaga gcttgattca tggctaaggt gttattaggg tttatggggg ctggaaaatc
     8221 gactattgca agaggcttgg accctaatta ccttgatatg gatgctctga ttgagaagcg
     8281 cctaggtatg tccattgcga attttttcgc tgaaaaggga gaagcagcct ttcgtcaggt
     8341 agaatcagaa gtcctagctg atttactaca aacagaccaa gtcgtgtcaa ctggaggagg
     8401 agtggttatt tctcagagaa atcgtgactt actcaagact aatacagata acatctacct
     8461 gaaagcagat tttgaaaccc tctacctacg tatcgtagct gataaggaca atcagcgacc
     8521 gctttttcta aataatagca aggaagaact agtagttatt tttcaagaaa gacaggcttg
     8581 gtatgaggaa gtggctagtc gggttttgga tgtgaccaag ctaagcccag aggaaattat
     8641 agaggaacta agatgaaaat tgcttatcta ggtcccaagg gatcattttc acaccacgtt
     8701 gtgcagacag cttttcctca tgaggaattg caggcttttg ccaacattac agatgtcatc
     8761 aaggcttatg agcaaggatt agtgaactat tctgtggtgc cagttgaaaa ttctattgag
     8821 ggtagtgttc atgaaacctt ggactatctt tttcatcagg ctcacatcca agcagtagca
     8881 gaaatcgttc agcctattca tcagcagttg atggtggttc caggccatac taagattgaa
     8941 aagatttttt cacatccaca ggccttggct caaggaaaga aattcatcga tgaacaatat
     9001 ccagaggctc aaatcgaggt aacagctagt acagcttatg cggcccgttt tatttccgaa
     9061 catccagacc agccctttgc agcagttgca cctagaagtt ctgctgaaga atatggattg
     9121 gaactgattg ccgaggatat tcaggaaatg gaagccaatt tcacacgttt ctgggttcta
     9181 ggagctgaaa agcctagtat tcccttgcaa gcacaaactg aaaagatgag tttggccttg
     9241 acattacctg acaaccttcc aggtgcactt tataaggccc tgtcgacctt tgcttggcga
     9301 ggaattgact tgacaaaaat tgaaagtcgt ccactcaaga cagcactggg tgaatacttt
     9361 ttcattatcg atgtggatta taccgataag gacttggtcc actttgccca aaaagaatta
     9421 gaagcgattg gaatccagta taaaattctg ggtgcctatc ctatttatcc aatatcagac
     9481 catggaaagg agagaagatg agtaaagaaa atcccttaag tcatcatgag cagttgcgtt
     9541 atgattattt gctaaaaaat attcactatc tcaatgagag agaaaaaaat gagtttgcct
     9601 atttgcaaga aaagctaact cttgctaggg gaaatagtag ctctagcttg gaacaagaaa
     9661 gagaagagca ggttgactta ccaagctatg cgaaccggag tcgctcacaa tccaaatcac
     9721 aagcactctc ttcccctcca aaaaagaaaa gacggaagct ccgtcttaaa cgaattttta
     9781 tggtgatttt ttcacttttg gtctgtgtgg ctttggccat ggtattcatg tttttgcgtg
     9841 gttaccaaga tgctagcgca aagaaaactg ctgatgcccg ggcagctcaa gtagaagtct
     9901 ttaatggtca ggacactaga gatggagtta atattttaat catgggtact gatggtcgaa
     9961 tcggccagaa cagtgttgag acacgaactg actctattat ggtattaaat gtcgggggct
    10021 cagataagaa aatgaagcta gtcagtttca tgcgtgataa tttggtctat atagatggtt
    10081 atagtcaagt gattaatggt agaaaacaga cagataacaa gttaaacgta gcctacgagt
    10141 taggagaaca agaggggcaa aaaggggcag aaatggttcg ccaagtcttg aaagataatt
    10201 ttgacttgga cattaagtac tacgccttgg ttgatttcca ggcctttgcc acagcgattg
    10261 acacgctttt ccctgatggg gtgacgattg atgctcaatt ttcaacattg aatgggcgtc
    10321 ccctaacaga agctacagtc ggagatgatt tatacgctac cgagactgag tctccaaccc
    10381 aaaccattaa agtcggaaaa cagcagatga atggctcgac cctgctcaat tatgctcgtt
    10441 tccgtgatga tgatgaggcg gattacggcc gtacaaaaag acagcaacaa gttttaacag
    10501 caattttaga gcaaattaaa gatcccacta aacttttcac tggttctgaa gctcttggaa
    10561 aggtttttgc tatgacctca accaacgtac cctatacttt cctcctaaca aacggtttat
    10621 ctgttttgga tggagcaaaa aatggtattg aaaaattgac gattccagaa ctgggtgact
    10681 gggtagatgc ctatgatgtt tatgggggct tgggcctgct ggttgatcaa aacaaatatc
    10741 aaaccaagct cgctcaaatg ggcttaagat aagataatac tcaataaaaa tcaaagtgca
    10801 aactaggaag ctagccgcag gctgctaaaa acaaagtttt gaggttgtag ataagactga
    10861 cgaagtcagt tacctatacc tacggcaagg tgaagctgac gtggtttgaa gagattttcg
    10921 aagagtataa gacagaaaaa tcaaagtttg atagctattt ggatagcagt caggctttga
    10981 ttttttacag tctgcaatag attttcaccc cttgtttgtg gtataatgaa acgatacgat
    11041 agaaagaata atgaacaata tgactgattt aaaagcaatt caggctcgta gtctggagat
    11101 ggctgaatat tttgtggcct tttgtaaaga acatgatttg ctctgttatc tctgtggcgg
    11161 aggcgctatt ggtgcccttc gaaacaaggg atttattcct tgggacgacg acctagactt
    11221 ttttatgcct cgtaaagatt atgagaaatt agcagaatta tggcctcgtt atgcagatga
    11281 acgttatttc ttgtcaaaga gtcacaagga ttttgttgat cgtaatcttt ttattaccat
    11341 tcgtgacaag gaaaccacct gtatcaagcc ttatcagcag gatttggatt tgccacatgg
    11401 tctggccttg gatattttgc ctttggatta ttatccgaaa aatccagctg agcggaaaaa
    11461 acaggttcgt tgggccttga tttattcact cttttgtgcg caaactattc cagaaaagca
    11521 tggtgctctc atgaaatggg gaagtcgcat tttactgggt ttgactccaa aatctctccg
    11581 ttatcgcatc tggaaaaaag ctgagaaaga aatgactaag tatgatttgg ctgattgtga
    11641 tggcattaca gaattatgct caggtcctgg ctacatgaga aacaagtacc caatcacatc
    11701 ttttgaagac aatcttttct tgccatttga aggaacagag atgcctattc caatcggcta
    11761 tgatgtctat ctcagaactg cttttgggga ttatatgacg cctccaccag cagacaagca
    11821 ggtaccgcat catgatgctg tcatcgctga tatggataag tcttatacag aatacaaggg
    11881 agaatatggt ggctaagaaa aaaatcttat tttttatgtg gtctttttct cttggaggtg
    11941 gtgcagagaa gattctatca accattgttt caaatctgga tccagaaaag tatgatattg
    12001 atattcttga aatggagcac tttgacaagg gatatgaatc tgttccaaag catgtacgca
    12061 ttttaaaatc ccttcaagat tatcgccaaa ccagatggtt acgagctttt ttgtggagaa
    12121 tgagaattta ttttccaaga ctggctcgtc gtttgcttgt aaaggatgat tatgatgttg
    12181 aagtttcttt taccattatg aatccaccac tgttgttctc taaaagaaga gaagtcaaga
    12241 agatatcttg gattcatgga agtattgaag aacttcttaa ggatagctct aaaagagaat
    12301 cacatagaag ccagttggat gctgcgaata caattgtagg gatttcaaaa aagaccagca
    12361 attctatcaa ggaagtttat ccagattata cttctaaatt acagacaatc tacaatggat
    12421 atgattttca gactattcta gaaaaatctc aagagaagat cgatatcgag attgctcctc
    12481 aaagtatctg tactatcgga cggattgagg aaaataaggg ttctgaccgt gtagtggaag
    12541 tgatacgatt attacaccaa gagggaaaaa actatcatct ctattttatc ggggctggtg
    12601 atatggaaga ggaactgaaa aaacgagtca aagagtatgg gattgaggac tatgtacatt
    12661 tccttggtta tcaaaaaaat ccttatcagt atctatctca gacgaaagtt cttttgtcta
    12721 tgtctaaaca agaaggtttt cctggagtgt atgtggaggc cttgagtctg ggactccctt
    12781 ttatctctac ggacgttgga ggggctgagg aattatccca agaaggacga tttggacaaa
    12841 tcattgagag caatcaagag gcagctcagg cgattactaa ttacatgact tctgcctcaa
    12901 actttgatgt cgatgaggct agccaattca ttcaacaatt tacaattaca aaacaaatcg
    12961 aacaagtaga aaaactatta gaggagtagc atggaaactg cattaattag tgtgattgtg
    13021 ccagtctata atgtggcgca gtacctagaa aaatcgatag cttccattca gaagcagacc
    13081 tatcaaaatc tggaaattat tcttgttgat gatggtgcaa cagatgaaag tggtcgcttg
    13141 tgtgattcaa tcgctgaaca agatgacagg gtgtcagtgc ttcataaaaa gaacgaagga
    13201 ttgtcgcaag cacgaaatga tgggatgaag caggctcacg gggattatct gatttttatt
    13261 gactcagatg attatatcca tccagaaatg attcagagct tatatgagca attagttcaa
    13321 gaagatgcgg atgtttcgag ctgtggtgtc atgaatgtct atgctaatga tgaaagccca
    13381 cagtcagcca atcaggatga ctattttgtc tgtgattctc aaacatttct aaaggaatac
    13441 ctcataggtg aaaaaatacc tgggacgatt tgcaataagc taatcaagag acagattgca
    13501 actgccctat cctttcctaa ggggttgatt tacgaagatg cctattacca ttttgattta
    13561 atcaagttgg ccaagaagta tgtggttaat actaaaccct attattacta tttccataga
    13621 ggggatagta ttacgaccaa accctatgca gagaaggatt tagcctatat tgatatctac
    13681 caaaagtttt ataatgaagt tgtgaaaaac tatcctgact tgaaagaggt cgcttttttc
    13741 agattggcct atgcccactt ctttattctg gataagatgt tgctagatga tcagtataaa
    13801 cagtttgaag cctattctca gattcatcgt tttttaaaag gccatgcctt tgctatttct
    13861 aggaatccaa ttttccgtaa ggggagaaga attagtgctt tggccctatt cataaatatt
    13921 tccttatatc gattcttatt actgaaaaat attgaaaaat ctaaaaaatt acattagaac
    13981 gggggtgacc aagtgattga tgggaaacga ttattattta gtttgaccat agtcagttat
    14041 gccttgacgc tagtaagtgg aattgtgtat ctgtttaata ataataatgt tagcttactt
    14101 tctactttat tgttcttgtt ggttagtagc ttaattgctt gttggaatga tatcaagtat
    14161 tacttaatcc attttatttt ctatttaacc atttttgtat ttctggtatc aagaccgacc
    14221 attgattatt ttagggatgg tgctttggat acctatcatc caatagccta tcgttttgcc
    14281 tttatagttg tcatgatttc gattctgggc ttgaccacag gaggcattct ggctcgttac
    14341 ttcatagcta ggaagaaaat aaaagtagta aatataggaa attctctaaa agaggtttat
    14401 atcaagcggt tacgctttgt atcactagga gtttttcttc taacttatcc tttctatttc
    14461 attcggttat ttgaacggct cttgtatcgt ttgcagactt cctactatgc ctactatgca
    14521 aattttgaaa gtaaactgcc ttattttacc tacattttgt ctacctttac ggtctatgca
    14581 atgtgtatgt atctggcaac caagccaaag aaattgcagg ccacagcagt gcttgtctcc
    14641 tttattgcag ctaatactat tcatttggca attgggacac gaaatccctt tattttaagt
    14701 attttatttg cttttgttta ttactttatg cgggagcaaa ctgaaaaagg aaaatggatt
    14761 gggtttaaag aaaagttagc gatttttgta ggttctccta ttctcatgtt agcgatggga
    14821 gtactcaatt atgtacggga taatgtccaa gtttcccata caggtttctg ggatatctta
    14881 cttgacttta tctataaaca agggactagt tttggtgttc tggctcgagg ttttctattt
    14941 aacagtagcc tcccttaccg agatttccgt aattttactt ttggtcctgt tcttgattat
    15001 tttgcaaggg ggagtttggg agccattttc ggaggaaaag cctttgaaca tacaaccaat
    15061 agtgtggaac tagctattga tagtaatagt tatgcccaca atctatccta tcttgtcttg
    15121 aacaaggaat acttgaaagg gcatggtatc ggaagtagtt atatcatgga gttgtatacc
    15181 gactatggta tgattggagt ctttctgctt agtttcttac tcggcgtatt atttatagcc
    15241 atgctgcaag tagcctatcg ctcaaggaca atcctatttg ctttatccct actcatcttg
    15301 aataatctat tctttatgcc aagaagcagc ttttcagaaa gtttcttcaa tttatttaca
    15361 atgcaattct ggggaattgt tcttgtgatt atatttgtag caaaaatgct tacaaaggaa
    15421 aactagtatc tactaaacaa aggagaaaaa aatcatgttt gaacattatt cagtagctga
    15481 tttgtttgca aatctttata aaaagcgaaa agcaaatatt ttagctctca tcgctttatt
    15541 tgctctcatt gctgtaccat ttacaattaa agcagttagg aataaaaaca ctgtcaaaga
    15601 tacaacaagt tattcaactt atcttatcta taaaatcact cctccaaaag agtcggccaa
    15661 aacgattttg aatcatcaaa ttggtggtta tagtgatttt tatgggaaat tgattgatgg
    15721 taatttgaat ggagcttatc ttttcaatga tgtagaatcc agtgagttga aaaaaattgc
    15781 cagtgaatta gacacgacag aaacaacctt gaaaaattca acgaatgact attggtggaa
    15841 aaaattgacc gtctactata tgattgacga tgcaggggtt ggtgtgaaaa ttttgacatc
    15901 aagtaaagat gccaatgact tgttagagaa aaaaattgat gggttgattg agaaatttaa
    15961 acatgcctat gcaaatgtga aaattgaaaa actggaaacc atcaactcta aagaattgaa
    16021 cgcaaatggt gaaacagcgc ttggcttaaa tgtgaaaaat ctgattcttc gtttagttgt
    16081 tattggagtg gtttgtgtga ttttggttgt gatgggaaat gtgttagttt atctctttaa
    16141 tccaacaatc aatagagtag gtgatttttc tcagtatcaa attgattttg taacagagat
    16201 tacaacaatt gctaacctag cagatgtttt gtcatacaaa aatactggac aggaattgac
    16261 catcgttagc tcaaataaag ctatcctaga taaattgaaa cagagtcaag aagctttaaa
    16321 aggaatgcat tttgtagatt tacaggatgt atcatctctt ttggaaagag atacagtcct
    16381 tcttgttgaa gagtacggag tgactcgtta taagaaattt gagcaaagtc ttcaaattct
    16441 cagaaactta aatcgttcta tccttggtgt agcaaccttt aaattataag tattctgatg
    16501 tataaggtct tcaaaatcgg ctaagagctg attttgaagg ctttttctat ctgtctgcag
    16561 aaattttctt ttggagtctc ctatagaaag accctaggga aaatctatcc gtatagagga
    16621 gccttctttt gataaaatcg taaaaatcta gtataataga tagaactgaa agtatgaggt
    16681 tactagcaat gaaaatgaaa caaattagtg atacaacttt aaaaatcacg atgtctttag
    16741 aggatttgat ggatcgtgga atggagattg ctgactttct cgttcctcaa gaaaaaacag
    16801 aagagttctt ttatgctatc ttggatgagc tagagatgcc tgatagcttt ctggatacag
    16861 gcatgttgag cttccgtgtg actccaaaac ctgataaggt agatgtcttt gtgaccaagt
    16921 caaagattga ccaaaatcta gattttgaag acttatcgga tttgccagat atggaagaat
    16981 tggctcaaat gtctccagat gaatttatca aaaccctgga aaaaagcatc gcagacaaaa
    17041 ccaaggatga tatcgaagcc attcaatctc ttgagcaagt tgaagccaag gaagaagagc
    17101 aagagcaggc tgaacaagaa gctgagagta agaaagaacc ttacatctac tacatccttt
    17161 cttttgctaa gttggctgac ttggtagttt ttgccaagac agtgactttt gaaatggaaa
    17221 cttctgaact ctacaaaatg aacgagcgct attatttgac cattttagtg gatattgaaa
    17281 atcatccaag cccatatcca gcttggctgt tggcccgtat gcgcgagttt gcagacgata
    17341 gtgatatcag tcgctcagtc ttacaagagt atggtcaagt cttgatgagt cacgatgcag
    17401 tgctcaatct gcaaaaaatc ggctaatcct ttctggaaag ctattttcag attttaagaa
    17461 gagcgaatcg tctgattcgt tttttctttt tatactgaaa tagtgattta ctataatagg
    17521 aattttcaca aaattctgtt ataatggcta tattagaaaa tttcgaggag ataaagatga
    17581 cagttaaaat tgctttacta ggatttggta ccgttgcaag tggtgtgcct ttcctcctaa
    17641 aggaaaatgg aggaaaaatc aatcaatcag cacattcaga tatcaaagtt gctaaggtat
    17701 tggtcaagga tgaagatgaa aaaaatcgct tgcttgcagc agggaatgac tttaactttg
    17761 taaccaatgt ggatgatatt ttatcagacc aggatattac tatcgtagtg gaattgatgg
    17821 ggcgtattga gcctgctaaa acctttatca ctcgtgcctt ggaagctgga aaacacgttg
    17881 ttactgctaa caaggacctt ttagctgtcc atggcgcaga attgctagaa atcgctcaag
    17941 ctaacaaggt agcactttac tacgaagcag cagttgctgg tgggattcca attcttcgta
    18001 ctttagcaaa ttccttggct tctgataaaa ttacgcgcgt gcttggagta gtcaacggaa
    18061 cttccaactt catggtgacc aagatggtgg aagaaggctg gtcttacgat gatgctcttg
    18121 cggaagcaca acgtctagga tttgcagaaa gcgatccgac gaatgacgta gatgggattg
    18181 atgcagccta caagatggtt attttgagcc aatttgcctt tggcatgaag attgcctttg
    18241 atgatgtagc ccacaaggga atccgcaata tcacaccaga agacgtagct gtagctcaag
    18301 agcttggtta cgtagtgaaa ttggttggtt ctattgagga aacttcttca ggtattgctg
    18361 cagaagtgac tccaaccttc ctacctaaag cgcacccact tgctagtgtg aatggcgtaa
    18421 tgaacgctgt ctttgtagaa tctatcggta ttggtgagtc tatgtactac ggaccaggtg
    18481 ctggtcaaaa accaactgca acaagtgttg tagctgatat tgtccgtatc gttcgtcgtt
    18541 tgaatgatgg tactattggc aaagacttca acgaatatag ccgtgacttg gtcttggcaa
    18601 atcctgaaga tgtcaaagca aactactatt tctcaatctt ggctctagac tcaaaaggtc
    18661 aggtcttgaa gttggctgaa atcttcaatg ctcaagatat ttcctttaag caaatccttc
    18721 aagatggcaa agagggtaac aaggcgcgtg tcgttatcat cacacacaag attaataaag
    18781 cccagcttga aaatgtctca gctgaattga agaaggtttc agaattcgac ctcttgaata
    18841 ccttcaaggt gctaggagaa taagatgaag attattgtac ctgcaaccag tgccaatatc
    18901 gggccaggtt ttgactcggt cggtgtagct gtaaccaagt atcttcaaat tgaggtctgc
    18961 gaagaacgag atgagtggct gattgaacac cagattggca aatggattcc acatgatgag
    19021 cgtaatctct tgctcaaaat cgctttgcaa attgtaccag acttgcaacc aagacgcttg
    19081 aaaatgacca gtgatgtccc tttggcgcgc ggtttgggtt cttccagctc ggttatcgtt
    19141 gctgggattg aactagccaa ccaactgggt caactcaact tatcagacca tgaaaaattg
    19201 cagttagcga ccaagattga agggcatcct gacaatgtgg ctccagccat ttatggtaat
    19261 ctcgttattg caagttctgt tgaagggcaa gtctctgcta tcgtagcaga ctttccagag
    19321 tgtgattttc tagcttacat tccaaactat gaattacgta ctcgcgacag ccgtagtgtc
    19381 ttgcctaaaa aattgtctta taaggaagct gttgctgcaa gttctatcgc caatgtagcg
    19441 gttgctgcct tgttggcagg agacatggtg accgctgggc aagcaatcga gggagacctc
    19501 ttccatgagc gctatcgtca ggatttggta agagaatttg cgatgattaa gcaagtgacc
    19561 aaagaaaatg gggcctatgc aacctacctt tctggtgctg ggccgacagt tatggttctg
    19621 gcttctcatg acaagatgcc aacaattaag gcagaattgg aaaagcaacc tttcaaagga
    19681 aaactgcatg acttgagagt tgatacccaa ggtgtccgtg tagaagcaaa ataaagaata
    19741 gaagatagga tggggaaact cttgaccaga gggtttcata tcctttttgt gaaaagaagt
    19801 ttatactcaa tgaaaatcaa agagcaaact aggaagctag ccgcaggctg ctcaaaacag
    19861 tgttttgagg ttgcagatag aactgacgaa gtcagctcaa gacactgttt tgaggttgca
    19921 gatagaactg acgaagtcag taacatacct acggtaaggt gacgctgacg tggtttgaag
    19981 agattttcga agagtattag ttaaaaacgt gataaaggag aaataaaaat ggcagaaatt
    20041 tatctagcag gtggttgttt ttggggccta gaggaatatt tttcacgcat ttctggagtg
    20101 ctagaaacca gtgttggcta cgctaatggt caagtcgaaa cgaccaatta ccagttgctc
    20161 aaggaaacag accatgcaga aacggtccaa gtgatttacg atgagaagga agtgtcactc
    20221 agagagattt tactttatta tttccgagtt atcgatcctc tatctatcaa tcaacaaggg
    20281 aatgaccgtg gtcgccaata tcgaactggg atttattatc aggatgaagc agatttgcca
    20341 gctatctaca cagtggtgca ggagcaggaa cgcatgctgg gtcgaaagat tgcagtagaa
    20401 gtggagcaat tacgccacta cattctggct gaagactacc accaagacta tctcaggaag
    20461 aatccttcag gttactgtca tatcgatgtg accgatgctg ataagccatt gattgatgca
    20521 gcaaactatg aaaagcctag tcaagaggtg ttgaaggcca gtctatctga agagtcttat
    20581 cgtgtcacac aagaagctgc tacagaggct ccatttacca atgcctatga ccaaaccttt
    20641 gaagagggga tttatgtaga tattacgaca ggtgagccac tcttttttgc caaggataag
    20701 tttgcttcag gttgtggttg gccaagtttt agccgtccga tttccaaaga gttgattcat
    20761 tattacaagg atctgagcca tggaatggag cgaattgaag ttcgttctcg ttcaggcagt
    20821 gctcacttgg gtcatgtttt cacagatgga ccgcgggagt taggcggcct ccgttactgt
    20881 atcaattctg cttctttacg ctttgtggcc aaggatgaga tggaaaaagc aggatatggc
    20941 tatctattgc cttacttaaa caaataaaac agagagtggg gcttcccact ttcttcattt
    21001 ctagaatatg aatagaaggg atttatgaaa cacctattat cttacttcaa accctacatc
    21061 aaggaatcaa ttttagcccc cttgttcaag ctgttagaag ctgtttttga gctcttggtt
    21121 cccatggtga ttgctgggat tgttgaccaa tctttacctc agggagatca aggtcatctc
    21181 tggatgcaga ttggcctgct ccttatcttt gcagtaattg gcgttttagt ggccttgata
    21241 gctcaatttt actcagcaaa ggcagcagta ggctttgcta aggaattgac aaacgatctt
    21301 tatcgtcata ttctttcctt gcccaaggac agcagagacc gtctgacaac ttctagtttg
    21361 gtcactcgct tgacttcgga tacctaccag attcagactg gtatcaatca attcctgcgt
    21421 ctctttttgc gagcgcccat tatcgttttt ggtgccattt ttatggctta tcgaatctca
    21481 gctgagttga ctttctggtt cttagtcatg gttgccattt tgaccattgt cattgtaggg
    21541 ttatctcgat tggtcaatcc tctctacagt agtctcagaa agaaaacgga ccaactggtt
    21601 caggaaacac gccagcaatt gcaagggatg cgggttattc gtgcttttgg tcaagaaaaa
    21661 cgagagttac agatttttca aacccttaac caagtttatg ctagattaca agaaaagaca
    21721 ggtttctggt ctagtttatt aacacctctg acctatctga ttgtcaatgg aactcttctc
    21781 gttattatct ggcaaggcta tatttcaatt caaggaggag tgctcagtca aggtgctctc
    21841 attgctctta tcaattacct cttacagatt ttggtggaat tggtcaagct agccatgttg
    21901 atcaattccc tcaaccagtc ctatatctca gccaagcgaa tcgaggaagt ctttgttgag
    21961 gctccagagg atatccattc agagttagaa caaaagcaag ctaccagaga taaggtttta
    22021 caagtccaag aattgacctt tacctatcct gatgcggccc agccttctct gagagacatt
    22081 tcctttgata tgactcaagg acaaatcctt ggtatcattg gggggactgg ttctggtaaa
    22141 tcaagcttgg tgcaactctt acttggactt tatccagtag acaaggggaa cattgacctt
    22201 tatcaaaatg gacgtagtcc tcttaatttg gagcagtggc ggtcttggat tgcctatgta
    22261 cctcaaaagg tcgaactctt taaaggaacc attcgttcca acttgactct aggtttcaat
    22321 caagaagtat ctgaccagaa actctggcag gccttggaga ttgcgcaagc taaggatttt
    22381 gtcagtgaaa aggaaggact cttggatgcc ctagttgagg caggggggcg aaatttctca
    22441 ggtggacaaa aacaaaggtt gtctatcgcc cgagcagtct tgcgccaagc tccgtttctc
    22501 atcctagatg atgcaacctc ggcactggat accattacag agtccaagct cttgaaagct
    22561 attagagaaa attttccaaa cacgagctta attttgatct ctcaacgaac ctcaacttta
    22621 cagatggcgg accagattct cctcttggaa aaaggtgagt tgctagctgt tggcaagcac
    22681 gatgacttga tgaaatccag ccaagtctat cgtgaaatca atgcatccca acatggaaag
    22741 gaggactaga atgaaacgac aaactgtaaa ccagacgctc aaacgtttag ccgtagattt
    22801 agcaaaccat cccttcctcc ttttcctagc ttttctagga actattgccc aagttggctt
    22861 atcaatttac ctacctattc tgattgggca ggtcattgac caagtcctag tggctggttc
    22921 atcaccagtt ttttggcaga tttttctcca gatgctcttg gtggtaatag gaaatactct
    22981 ggtacaatgg gccaatcctc tcctctataa tcgtctaatc ttctcttata ccagagattt
    23041 acgagagcga atcatccata aactccatcg gttaccgatt gcttttgcgg atcggcaagg
    23101 caatggagag atggtcagtc gtgtaaccac ggacatcgaa cagttggcag ctggcttgac
    23161 catgattttt aaccaatttt tcattggtgt tttgatgatt ttggttagta ttctagccat
    23221 gctccaaatt catctcctca tgactctctt agtcttgctg ttgacgccac tgtccatggt
    23281 gatttcacgc tttattgcca agagatccta tcatctcttc cagaagcaaa cagagacgag
    23341 ggggattcag acccagttga ttgaagaatc gcttagccag cagactataa tccagtcctt
    23401 caatgctcaa acagagttta tccaaagatt gcgtgaggct catgacaact actcaggcta
    23461 ttctcagtca gccatctttt attcttcaac ggtcaatcct tcgactcgct ttgtaaatgc
    23521 actcatttat gcccttttag ctggagtagg agcttatcgt atcatgatgg gttcaacctt
    23581 gaccgtcggt cgtttagtga cttttttgaa ctatgttcag caatacacca agccctttaa
    23641 cgatatttct tcagtgctag ctgagttgca aagtgctctg gcttgcgtag agcgtatcta
    23701 tggagtctta gatagccctg aagtggctga aacaggtaag gaagtcttga ccagtgacca
    23761 agttaaggga gctatttcct ttaaacatgt ctcttttggc taccatcctg aaaaaatttt
    23821 gattaaggac ttgtctatcg atattccagc tggtagtaag gtagccatcg ttggtccgac
    23881 aggtgctgga aaatcgactc ttatcaatct ccttatgcgt ttttatccca ttagctcggg
    23941 agatatcttg ctggatgggc aatccattta tgattataca cgagtatcat tgagacagca
    24001 gtttggtatg gtgcttcaag aaacctggct cacacaaggg accattcatg ataatattgc
    24061 ctttggcaat cctgaagcca gtcgagagca agtaattgct gctgccaaag cagctaatgc
    24121 agactttttc atccaacagt tgccacaggg atacgatacc aagttggaaa atgctggaga
    24181 atctctctct gtcggccaag ctcagctctt gaccatagcc cgagtctttc tggctattcc
    24241 aaagattctt atcttagacg aggcaacttc ttccattgat acacggacag aagtgctggt
    24301 acaggatgcc tttgcaaaac tcatgaaggg ccgcacaagc ttcatcattg ctcaccgttt
    24361 gtcaaccatt caggatgcgg atttaattct tgtcttagta gatggtgata ttgttgaata
    24421 tggtaaccat caagaactca tggatagaaa gggtaagtat taccaaatgc aaaaagctgc
    24481 ggcttttagt tctgaataag ccattctctt ttgaaagttt atggacgaaa aaagttgcct
    24541 tcgagtgact tttttgttac aatagctaga aaaattgttc actgtaatac tcaatgaaaa
    24601 tcaaagagca aactaggaag ctagccgtag gttgctcaaa gcacagcttt gaggttgcag
    24661 atagaactga cgaagtcagt aaccacacct acggcaaagt gaatctgaag tggtttgaag
    24721 agagtacaac ttgtctttta gaaaaggagc ctataatgaa agtctttcag catgtaaata
    24781 tcgtgacttg tgatcaagat ttccatgttt atcttgatgg aatcttagca gtcaaggatt
    24841 ctcaaatcgt ctatgtcggt caagataagc cagcgttttt agagcaagct gagcagatta
    24901 tagactatca gggagcttgg attatgcctg gtttggtcaa ttgtcacacc cattctgcaa
    24961 tgacaggtct gagagggatc cgagatgaca gcaatctcca tgaatggctc aatgactata
    25021 tctggccagc agaatctgag tttactcccg acatgactac caatgcggtc aaagaagccc
    25081 tgacagagat gctccagtca ggaacaacaa cctttaacga tatgtataat cccaatggtg
    25141 tggatatcca gcaaatttat caggtggtga aaacttccaa gatgcgttgt tatttttctc
    25201 cgactctctt ttcttcagag acagaaacaa ctgctgagac tataagcaga actcgatcca
    25261 tcatagacga aatcttaaaa tataaaaatc caaatttcaa ggttatggta gcacctcatt
    25321 ctccgtatag ctgcagtaga gacttgctgg aagcgagttt ggaaatggca aaagagctaa
    25381 atattccgct ccatatccat gtggcggaga ccaaggagga gtcaggaatt atcctcaaac
    25441 gatacggcaa acgccccctt gcttttcttg aagaactggg ttatttagat catccgtccg
    25501 tatttgctca cggggtcgaa ttaaacgaga gagaaattga acgcttggca tcttctcaag
    25561 tggctatcgc ccacaatcct atcagtaacc tcaaactggc atcaggaatt gctccaatta
    25621 tccagctcca aaaagcggga gtagcagtcg gaattgcgac tgactcggtt gcttccaata
    25681 acaatctaga tatgtttgag gaaggaagga ctgcagctct tcttcagaag atgaaaagtg
    25741 gggatgccag ccagtttcca atcgaaacag ctctcaaggt actgacaatc gaaggggcta
    25801 aggtccttgg aatggaaaat cagataggaa gtctggaagt cggcaagcaa gcagattttc
    25861 tggtcattca accacaaggg aaaattcatc tccaacctca ggaaaatatg ctgtctcacc
    25921 tggtttatgc agttaaatct agtgatgtag atgatgttta tatcgccgga gaacaggttg
    25981 ttaagcaagg tcaagtcctg acagtagaac tttaaaagaa aaatcacgaa aaattttaaa
    26041 aaaagtttgc aaaaatcttg cattcttttt ttgactatgc tatacttata tacggtttaa
    26101 aaaaactgcc taagacagta ggggagatcg actcataaat atcctaccga ggacaaaacg
    26161 tatcatgtaa aaagaagcgt attgtacttt cgtgtctagg tttgggcgcg tttttctttt
    26221 tgaaaaattc cccaagcaaa ataattacgg aggtgaacac actaatgagt gaagcaatta
    26281 ttgctaaaaa agcggaacta gttgacgtag tagctgaaaa aatgaaagct gctgcatcta
    26341 tcgtcgttgt agacgctcgt ggtttgacag ttgagcaaga tacagttctt cgtcgtgagc
    26401 ttcgtggaag cgaagttgag tataaagtta ttaaaaactc aatcttgcgt cgtgcagctg
    26461 aaaaagctgg tcttgaagat cttgcatctg tatttgttgg accatctgca gtagcatttt
    26521 ctaatgaaga tgttatcgca ccagcgaaaa tcttgaacga cttttctaaa aacgctgaag
    26581 cacttgaaat taaaggtggt gcaatcgaag gcgctgtcgc atctaaagaa gagattcttg
    26641 cacttgcaac tcttccaaac cgcgaaggac ttctttctat gctcctttct gtacttcaag
    26701 cgccagtgcg caacgttgct cttgcagtca aagcggttgc agaaagcaaa gaagacgcgg
    26761 cttaatctta agctacatag cgtagcctag ctacgaaaaa aactattata aaatttaaaa
    26821 cttatttgga ggaaataaca atggcattga acattgaaaa cattattgct gaaattaaag
    26881 aagcttcaat ccttgaattg aacgaccttg taaaagctat cgaagaagaa tttggtgtaa
    26941 ctgcagctgc tcctgtagct gttgctgcag ctgatgcagc tgatgctggt gctgctaaag
    27001 attcattcga cgttgaattg acatctgcag gcgacaaaaa agttggcgtt atcaaagttg
    27061 tacgtgaaat cactggtctt ggtcttaaag aagctaaaga acttgttgac ggtgcaccag
    27121 cacttgttaa agaaggcgtt gcaactgcag aagctgaaga aatcaaagct aaattggaag
    27181 aagctggagc ttcagttact cttaaataat agagcaacta cattagtagc ttaaaaacat
    27241 gattaaaccg ctattcttag gagtagcggt ttttcttttt gtttaaaaag gggcaaactt
    27301 tttatatagt tgtttaggaa ataaataatt tttgtataat tg
//
LOCUS       DAVLVP010000019        25176 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.019, whole genome shotgun sequence.
ACCESSION   DAVLVP010000019 DAVLVP010000000
VERSION     DAVLVP010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 25176)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25176)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25176
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.019"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..191
                     /locus_tag="VYN69_000776"
     CDS             <1..191
                     /locus_tag="VYN69_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1533082.1"
                     /translation="MIAPDKEVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDL
                     SKISEPEKLYAYIAEATA"
     gene            229..534
                     /locus_tag="VYN69_000777"
     CDS             229..534
                     /locus_tag="VYN69_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1533083.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFRIPAMSAPVFDNIEAAIKEHGLS"
     gene            582..2057
                     /locus_tag="VYN69_000778"
     CDS             582..2057
                     /locus_tag="VYN69_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEV1533084.1"
                     /translation="MDAIFDLIGKVFNPILEMGGPVIMLIILTVLALLFGVKFSKALE
                     GGIKLAIALTGIGAIIGMLNGAFSASLAKFVENTGIQLSITDVGWAPLATITWGSAWT
                     LYFLLIMLIVNVVMLAMKKTDTLDVDIFDIWHLSITGLLIKWYADNNGVSQGVSLFIA
                     TAAIVLVGVLKIINSDLMKPTFDDLLNAPSSSPMTSTHMNYMMNPVIMVLDKIFEKFF
                     PGLDKYDFDAAKLNKKIGFWGSKFFIGFILGIVIGIMGTPHPIAGVADADKWRLVIKG
                     WLSLGLTAGVSLELFSLIGSWFIAAVEPLSQGITNVATKRLQGRKFNIGLDWPFIAGR
                     AEIWACANVLAPIMLIEAVLLSKVGNGILPLAGIIAMGVTPALLVVTRGKLLRMIIFG
                     TLLLPLFLLSGTLIAPFATELAKGVGAFPEGVSQTQLITHSTLEGPIEKLLGWTIGNT
                     TTGDIKAILGAVAFLVFYIGIFAWYRKQMIKRNEEYAAKAK"
     gene            2083..2160
                     /locus_tag="VYN69_000779"
                     /pseudo
     CDS             2083..2160
                     /locus_tag="VYN69_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            2362..9063
                     /locus_tag="VYN69_000780"
     CDS             2362..9063
                     /locus_tag="VYN69_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HEV1533085.1"
                     /translation="MGKGHWNRKRVYSIRKFAVGACSVMIGTCAVLLGGNIAGESVVY
                     ADETLITHTAEKPKEEKMIVEEKADKALETKNVVERTEQSEPSSTEAIASEKKEDEAV
                     TPKEEKVSAKPEEKAPRIESQASSQEKPLKEDAKAVTNEEVNQMIEDRKVDFNQNWYF
                     KLNANSKEAIKPDADVSTWKKLDLPYDWSIFNDFDHESPAQNEGGQLNGGEAWYRKTF
                     KLDEKDLKKNVRLTFDGVYMDSQVYVNGQLVGHYPNGYNQFSYDITKYLHKDGRENVI
                     AVHAVNKQPSSRWYSGSGIYRDVTLQVTDKVHVEKNGTTILTPKLEEQQHGKVETHVT
                     SKIVNTDDKDHELVAEYQIVERGGHAVTGLVRTASRTLKAHESTSLDAILEVERPKLW
                     TVLNDKPALYELITRVYRDGQLVDAKKDLFGYRYYHWTPNEGFSLNGERIKFHGVSLH
                     HDHGALGAEENYKAEYRRLKQMKEMGVNSIRTTHNPASEQTLQIAAELGLLVQEEAFD
                     TWYGGKKPYDYGRFFEKDATHPEARKGEKWSDFDLRTMVERGKNNPAIFMWSIGNEIG
                     EANGDAHSLATVKRLVKVIKDVDKTRYVTMGADKFRFGNGSGGHEKIADELDAVGFNY
                     SEDNYKALRAKHPKWLIYGSETSSATRTRGSYYRPERELKHSNGPERNYEQSDYGNDR
                     VGWGKTATASWTFDRDNAGYAGQFIWTGTDYIGEPTPWHNQNQTPVKSSYFGIVDTAG
                     IPKHDFYLYQSQWVSVKKKPMVHLLPHWNWENKELASKVADSEGKIPVRAYSNASSVE
                     LFLNGKSLGLKTFNKKQTSDGRTYQEGANANELYLEWKVAYQPGTLEAIARDESGKEI
                     ARDKITTAGKPAAVRLIKEDHAIAADGKDLTYIYYEIVDSHGNVVPTANNLVRFQLHG
                     QGQLVGVDNGEQASRERYKAQADGSWIRKAFNGKGVAIVKSTEQAGKFTLTAHSDLLK
                     SNQVTVFTGKKEGQEKTVLGTEVPKVQTIIGEAPEMPTTVPFVYSDGSRAERPVTWSS
                     VDVSKPGIVTVKGMADGREVEARVEVIALKSELPVVKRIAPNTDLNSVDKSVSYVLTD
                     GSVEEYEVDKWEIAEEDKAKLAIPGSRIQATGYLEGQPIHATLVVEEGNPAAPAVPTV
                     TVGGEAVTGLTSQKPMQYRTLAYGAKLPEVTASAKNAAVTVLQASAANGMRASIFIQP
                     KDGGPLQTYAIQFLEEAPKIAHLSLQVEKADSLKEDQTVKLSVRAHYQDGTQAVLPAD
                     KVTFSTSGEGEVAIRKGMLELHKPGAVTLNAEYEGAKGQVELTIQANTEKKIAQSIRP
                     VNVVTDLHQEPSLPATVTVEYDKGFPKTHKVTWQAIPKEKLDSYQTFEVLGKVEGIDL
                     EARAKVSVEGIVSVEEVSVTTPIAEAPQLPESVRTYDSNGHVSSAKVAWDAIRPEQYA
                     KEGVFTVNGRLEGTQLTTKLHVRVSAQTEQGANISDQWTGSELPLAFASDSNPSDPVS
                     NVNDKLISYNNQPANRWTNWNRSNPEASVGVLFGDSGILSKRSVDNLSVGFHEDHGVG
                     APKSYVIEYYVGKTVPTAPKNPSFVGNEDHVFNDSANWKPVTNLKAPAQLKAGEMNHF
                     SFDKVETYAVRIRMVRADNKLGTSITEVQIFAKQVAAAKQGQTRIQVDGKDLANFNPD
                     LTDYYLESVDGKVPAVTASVSNNGLATVVPSVREGEPVRVIAKAENGDILGEYRLHFT
                     KDKNLLSHKPVAAVKQARLLQVGQALELPTKVPVYFTGKDGYETKDLSVEWEEVPAEN
                     LTKAGQFTVRGHVLGSDLVAEVTVRVTDKLGEALSDNPNYDENSNQAFASATNDIDKN
                     SHDRVDYLNDGDHSENRRWTNWSPTPSSNPEVSAGVIFRENGKIVERTVAQAKLHFFA
                     DSGTDAPTKLVLERYVGPEFEVPTYYSNYQAYDADHPFNNPENWEAVPYRADKDIAAG
                     DEINVTFKAVKAKAMRWRMERKADKSGVAMIEMTFLAPSELPQESTQSKILVDGKELA
                     DFAENRQDYQITYKGQRPKVSVEENNQVASTVVDSGEDSLPVLVRLVSESGKQVKEYR
                     IHLTKEKPVSEKTVAAVQEDLPKLEFVEKDLAYKTVEKKDSTLYLGETRVEQEGKTGK
                     ERIFTAINPDGSKEEKLREVVEAPTDRIVLVGTKPVAQEAKKPQVSEKADTKPIDSSE
                     ASQTNKAQLPNTGSAASQAAVAAGLALLGLSAGLVVTKGKKED"
     gene            complement(9311..10167)
                     /locus_tag="VYN69_000781"
                     /pseudo
     CDS             complement(9311..10167)
                     /locus_tag="VYN69_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007722232.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(10378..10737)
                     /locus_tag="VYN69_000782"
     CDS             complement(10378..10737)
                     /locus_tag="VYN69_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEV1533086.1"
                     /translation="MGMILMKLASILLLILTLVVCIILTKLFRLKKLGRNFADLAFPV
                     LVFEYYLITAKTFTHNFLPRLGLALSFLAIILVFFFLLKKRSFYYPKFIKFFWRAGFL
                     LTLIMYIEMIVELFLMK"
     gene            10829..11785
                     /locus_tag="VYN69_000783"
                     /pseudo
     CDS             10829..11785
                     /locus_tag="VYN69_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002881980.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein.; internal stop;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
     gene            11769..12326
                     /locus_tag="VYN69_000784"
     CDS             11769..12326
                     /locus_tag="VYN69_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1533087.1"
                     /translation="MKRPLEMAHDFLAEVVTKEDVVVDATMGNGHDTLFLAKLAKQVY
                     AFDIQKQALEKTQERLHQADLTNAQLILQGHETLDQFVIKAKAGIFNLGYLPSADKSV
                     ITRPQTTIEALEKLCGLLVKGGRIAIMIYYGHEGGDLERDAVLDFVIQLNQQEYTAAI
                     YRTLNQVNNPPFLVMIEKLERYRHG"
     gene            12319..12573
                     /locus_tag="VYN69_000785"
     CDS             12319..12573
                     /locus_tag="VYN69_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533088.1"
                     /translation="MDKQYLHEKLDAMRQNFVESTHHERAVGVLDQAHMSKKMLKIKK
                     KLVALEMERCQRKIEHKDCSKIDQKIKEQKEIFESCCKKD"
     gene            12585..14639
                     /locus_tag="VYN69_000786"
     CDS             12585..14639
                     /locus_tag="VYN69_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEV1533089.1"
                     /translation="MELLIYLILFLLVLIVSSTTNKLLPFLPLPLVQILLGIVIGLFL
                     PNTDFHLNTELFLALVIGPLLFREAEEADVTAILKHWRIIVYLIFPVIFISTLSLGGL
                     AHLLWFSLPLAACLAVGAALGPTDLVAFASLSERFSFPKRVSNILKGEGLLNDASGLV
                     AFQVALTAWTTGAFSLGQASSSLIFSILGGFLIGFLTAMTNRFLHTFLLSVRATDIAS
                     ELLLELSLPLVTFFLAEEVHVSGIIAVVVAGILKASRFKKITLLEAQVDTVTETVWHT
                     VTFMLNGSVFVILGMELEMIAEPILTNPIYNPLLLLLSLIALTFVLFVIRFIMIYGYY
                     AYRTRRLKKKLNKYMKDMFLLTFSGVKGTVSIATILLIPSNLEQEYPLLLFLVAGVTL
                     VSFLTGLLVLPHLSDEEEESKDYLMHIAILNEVTLELEKELEDTRNKLPLYAAIDNYH
                     GRIENLILSQENQDDQEDWAALKLLILSIESDGLEQAYEEGNISNRVYRVYQRYLKNI
                     EQGINRKLASRLTYYFLVSLRILRFLLHEVFTLGKTFRSWKNKEQSRLRALDYDQIAE
                     LYLANTEMIIESLENLKGVYSRSLISFMQESRLRETTIISSGAFVERVINRVKPNNIN
                     EMLRGYYLERKLIFEYEEKRLITTKYAKKLRQNVNNLENYSLKEAANTLPYDMVELVR
                     RN"
     gene            14818..15678
                     /locus_tag="VYN69_000787"
     CDS             14818..15678
                     /locus_tag="VYN69_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEV1533090.1"
                     /translation="MKKWWKELIDKPLLKAFLHYYQASDSELTSVAVAYYWLISIFPL
                     LLVVVNILPYFQIPVGEFLGFMKDVLPPSLYEGVEKIAREVLTQPSTGLLSFSVLSAL
                     WSFSKSMNFLQKAFNKAYGVEKSRGLISHQMLSLLVSFGLQILFAFALFLILFGQMIL
                     ALLAHYWTKDGIIYQALQGLAGPLIYALLFAILVMLYYFLPNLSNRKISYTLPGSAFV
                     LLVILGLLTLFSSYLNYYVHHLVDVRILGSVLLVVMMFWFILIAKIVILGAVINASMQ
                     SLKDPVFKKD"
     gene            15889..16599
                     /gene="ccdA2"
                     /locus_tag="VYN69_000788"
     CDS             15889..16599
                     /gene="ccdA2"
                     /locus_tag="VYN69_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1533091.1"
                     /translation="MGHIFFFLSVFLAGILSFFSPCILPLLPVYTGVLLDDKDGAQAS
                     SGKFSISVTSLLRTLAFIAGISFIFILLGYGAGFLGDLLYASWFQYLTGAIIILLGLH
                     QMEILHFKELYKEKRLQLQGQGQNGKGYSQAFLLGLTFSFAWTPCVGPVLGSVLALAA
                     SGGSGAWQGAGLMLVYTLGLALPFLLLALTSSYVLKHFRKLHPYLGILKKVGGFLIIV
                     MGLLVLFGNASILSQLFE"
     gene            16619..17185
                     /locus_tag="VYN69_000789"
     CDS             16619..17185
                     /locus_tag="VYN69_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin family protein"
                     /protein_id="HEV1533092.1"
                     /translation="MKKWQTCVLGAGSLLCLTACSGKSVTSEHQTKDEMKTEQTASKT
                     SAAKGKEVADFELMGVDGKTYRLSDYKGKKVYLKFWASWCSICLASLPDTDEIAKEAG
                     DDYVVLTVVSPGHKGEQSEADFKNWYKGLDYKNLPVLVDPSGKLLETYGVRSYPTQAF
                     IDKEGKLVKTHPGFMEKDAILQTLKELA"
     gene            17196..18308
                     /gene="msrB"
                     /locus_tag="VYN69_000790"
     CDS             17196..18308
                     /gene="msrB"
                     /locus_tag="VYN69_000790"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEV1533093.1"
                     /translation="MNDKLKIFLLLGVFFLAITGFYVLLIRNAGQTDASQIEKAAVSQ
                     GGKAVKKTEISKDADLHEIYLAGGCFWGVEEYFSRVPGVTDAVSGYANGRGETTKYEL
                     INQTGHAETVHVTYDAKQISLKEILLHYFRIINPTSKNKQGNDVGTQYRTGVYYTDDK
                     DLEVINQVFDEVAKKYDQPLAVEKENLKNFVVAEDYHQDYLKKNPNGYCHINVNQAAY
                     PVIDASKYPKPSDEELKKTLSPEEYAVTQENQTERAFSNRYWDKFESGIYVDIATGEP
                     LFSSKDKFESGCGWPSFTQPISPDVVTYKEDKSYNMTRMEVRSRVGDSHLGHVFTDGP
                     QDKGGLRYCINSLSIRFIPKDQMEEKGYAYLLDYVD"
     gene            18576..19313
                     /locus_tag="VYN69_000791"
     CDS             18576..19313
                     /locus_tag="VYN69_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1533094.1"
                     /translation="MTYTILIVEDEYLVRQGLTKLVNVAAYDMEIIGQAENGRQAWEL
                     IQKQVPDIILTDINMPHLNGIQLASLVRETYPQVHLVFLTGYDDFDYALSAVKLGVDD
                     YLLKPFSRQDIEEMLGKIKQKLDKEEKEEQLQDLLTNRFEGNMAQKIQSHLADSQFSL
                     KSLASDLGFSPTYLSSLIKKELGLPFQDYLVRERVKQAKLLLLTTDLKIYEIAEKVGF
                     EDMNYFTQRFKQIAGVTPRQFKKGEDR"
     gene            19310..21001
                     /locus_tag="VYN69_000792"
     CDS             19310..21001
                     /locus_tag="VYN69_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1533095.1"
                     /translation="MKRSSLLVRMVISIFLVFLILLALVGTFYYQSSSSAIEATIEGN
                     SQTTISQTSHFIQSYIKKLETTSTGLTQQTDVLAYAENPSQDKVEGIRDLFLTILKSD
                     KDLKTVVLVTKSGQVISTDDSVQMKTSSDMMAEDWYQKAIHQGAMPVLTPARKSDSQW
                     VISVTQELVDAKGANLGVLRLDISYETLEAYLNQLQLGQQGFAFIINENHEFVYHPQH
                     TVYSSSSKMEAMKPYIDTGQGYTPGHKSYVSQEKIAGTDWTVLGVSSLEKLDQVRSQL
                     LWTLLGASVTSLLVCLCLVWFSLKRWIAPLKDLRETMLEIASGAQNLRAKEVGAYELR
                     EVTRQFNAMLDQIDQLMVAIRSQEETTRQYQLQALSSQINPHFLYNTLDTIIWMAEFH
                     DSQRVVQVTKSLATYFRLALNQGKDLICLSDEINHVRQYLFIQKQRYGDKLEYEINEN
                     VTFDNLVLPKLVLQPLVENALYHGIKEKEGQGHIKLSVQKQDSGLVIRIEDDGVGFQD
                     AGDSSQSQLKRGGVGLQNVDQRLKLHFGANYQMKIDSRPQKGTKVEIYINRIETS"
     gene            21117..21680
                     /locus_tag="VYN69_000793"
     CDS             21117..21680
                     /locus_tag="VYN69_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEV1533096.1"
                     /translation="MNETQIQRETRQVVEDVLEKTNLKQGALFVLGLSSSEVLGGQIG
                     KESSQEIGELIVETILGILSSRGIHLAVQGCEHVNRALVVERQVAEQFGLEIVSVHPT
                     LHAGGSGQLAAFKFMQDPVEVEFIKAHAGLDIGDTAIGMHVKHVQVPIRPILREIGHA
                     HVTALASRPKLIGGARAHYPQDAIRKS"
     gene            21702..>25176
                     /locus_tag="VYN69_000794"
     CDS             21702..>25176
                     /locus_tag="VYN69_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIVAR domain-containing protein"
                     /protein_id="HEV1533097.1"
                     /translation="MFKKDRFSIRKIKGVVGSVFLGSLLMAPSVVDAATYHYVNKEII
                     SQEAKDLIQTGKPDRNEVVYGLVYQKDQLPQTGTEASVLTAFGLLTVGSLLLIYKRKK
                     IASVFLVGAMGLVVLPSAGAVDPVATLALASREGVVEMEGYRYVGYLSGDILKTLGLD
                     TVLEETSAKPGEVTVVEVETPQSTTNQEQARTENQVVETEEAPKTEESPKEEPKSEIK
                     PTDDTLPKVEEGKEDSAEPAPVEEVGGEVESKPEEKVAVKPESQPSDKPAEESKVEPP
                     VEQAKGPEQPVQPTQAEQPRIPKDSSQPEDPKEDRGAEDTPKQEDTPKQEDTQPEVVE
                     TKDEAANQPVEEPKVETPAVEKQTEPKVEQVGEPVEPSEDEKAPVSPEKQPEAPEEKA
                     VEETPKPEDKIKGIGTKEPVDKSELNNQIDKASSVSPTDYSTASYNALGPVLETAKGV
                     YASEPVKQPEVNSETNKLKTAIDALNVDKSELQEQLRVAEQKQQADYSAKTWREFKIA
                     ELQAKEINNQTTPLPKQSEIDAATKALQDALQALAVDKTVLQNAINTANSKREEEYTA
                     QTWKALEDALTAVNPVNEDETATQSKVDEATRNLEEAINNLVLLTEKPVLTFIETDKK
                     ALEREVVAKYSLENQNKTKIKSITATLKKGETVVSTVELIGDDVTNETITSAFKNLEY
                     YKEYTLSTTMVYDRGDGDVTEILDNQPIQLDLKKVELKNIKRTDLIKYENGKETNESL
                     ITTVPDDKRNYYLKITSKNQKTTLLAVKNIEETTVNGTPVYKVTAIADNLVSRTADNK
                     FEEEYVHYIEKPKVHEDNVYYNFKELVEAIQNDPSKEYRLGQSMSARNVVPNGKSYIT
                     KEFTGKLLSSEGKQFAITELEHPLFNVITNATINNVNFENVEIERSGQDNIASLANTM
                     KGSSVITNVKITGTLSGRNNVAGFVNNMNDGTRIENVAFFGKLHSTSGNGSHTGGIAG
                     TNYRGIVRKAYVDATITGNKTRASLLVPKVDYGLTLDHLIGTKALLTESVVKGKIDVS
                     NPVEVGAIASKTWPVGTVSNSVSYAKIIRGEELFGSNDVDDSDYASAHIKDLYAVEGY
                     SSGNRSFRKSKTFTKLTKEQADAKVTTFNITADKLESDLSPLAKLNEEKAYSSIQDYN
                     AEYNQAYKNLEK"
BASE COUNT         7829 a         4727 c         5496 g         7124 t
ORIGIN      
        1 atatgatagc tcctgataag gaagtagaag tatccttgct cttctttatc atcaataact
       61 caagttcaag tcaaacaaat attctggctc agttgatgga ctttttcaca ggaaatggac
      121 atcttgaaga cctatcaaaa atttccgaac cagaaaaact ttatgcttac attgctgaag
      181 caaccgctta atcttgtcta ttaaaaaata aaatcggagg aaatccaaat gattaaaatt
      241 cttgctgcct gcggtgcagg tgttaactca agtcaccaaa ttaaaagtgc tctagaagaa
      301 gaactttcaa accgtggtta tgatgttcac tgtgatgcag tcatggtgaa agatgtaaac
      361 gaagacctta tgaaaggtta cgatatcttt acaccaatcg ctgcaacaga ccttggtttt
      421 gaaccaggta tcccagttat cgaagctgga ccaatcttat tccgtatccc agcaatgagc
      481 gctccagtat ttgacaatat tgaagcagct attaaagaac acggattaag ctaattctaa
      541 tttgttaaca ttcatataac ataaaaaagg gaggaactgt tatggatgca atctttgacc
      601 taatcggaaa ggttttcaat cccatcttag aaatgggtgg acctgtcatc atgttaatca
      661 ttttgacagt attggcttta ctttttggag tgaaattctc caaagcgctt gaaggtggta
      721 tcaaacttgc catcgctctt acaggtatcg gtgctatcat cggtatgcta aatggtgctt
      781 tctcagcatc acttgcaaaa ttcgttgaaa acactggtat acaattgagt attaccgacg
      841 ttggttgggc accactcgca acaatcactt ggggttctgc ttggacacta tacttcttgc
      901 tcatcatgtt gattgtcaac gtagtgatgc ttgctatgaa gaaaaccgat acacttgatg
      961 tcgatatctt tgatatctgg cacttgtcta tcacaggtct cttgattaaa tggtatgctg
     1021 ataacaatgg tgtgagtcaa ggggtttcac tctttattgc tacagcagct atcgtccttg
     1081 tcggtgtgtt gaaaattatc aactctgact tgatgaaacc tacatttgat gaccttctta
     1141 acgccccaag ttcatcacca atgacatcaa ctcacatgaa ctacatgatg aacccagtta
     1201 tcatggtttt ggataagatt tttgaaaaat tcttcccagg ccttgataaa tatgactttg
     1261 atgctgctaa attgaacaag aaaatcggtt tctggggatc taaattcttc atcggtttca
     1321 tccttggtat cgttattggt attatgggaa ctccacatcc aattgcaggt gttgcagatg
     1381 cagataaatg gcgtcttgtt atcaaaggat ggttgtctct tggtttgact gccggtgtat
     1441 ctttggaact cttctcactt atcggttcat ggttcatcgc agccgtagaa ccactatcac
     1501 aaggtattac aaacgttgct actaaacgtc ttcaaggacg taaattcaat atcggtcttg
     1561 actggccatt catcgctggt cgtgctgaaa tctgggcttg tgccaacgta cttgcaccaa
     1621 tcatgttgat tgaagcagtg cttctttcaa aagttggaaa tggtatcttg ccacttgcag
     1681 gtatcatcgc tatgggtgtt actccagctc tcttggttgt aactcgtggt aaattgctcc
     1741 gtatgattat cttcggaaca ctcttgttgc cactcttcct tctttcaggt acacttattg
     1801 caccatttgc aacagaactt gctaaaggtg taggtgcctt cccagaaggt gtgagccaaa
     1861 ctcaattgat tactcactct actcttgaag gaccaatcga aaaacttctt ggttggacaa
     1921 ttggtaacac tacaactggt gatatcaaag caatccttgg tgcagtagcc ttccttgtat
     1981 tctatatcgg tatctttgct tggtacagaa aacaaatgat caaacgtaac gaagagtacg
     2041 cagcaaaagc aaaataatgc gctcctataa aatataaact caatgaaaat caaagagcaa
     2101 actaggaagc tagccgcagg ttgctcaaaa cacagttttg aggttgtaga taagactgac
     2161 gaagtcagct caaaacatgg ttttgaggtt gtagatgaaa ctgacgaagt cagtaatcat
     2221 atctacggca aggcgacact gacgaagttt gaattggatt ttcgcagagt gtaaattgtt
     2281 gaaactaggt tgtcatacca tgtataccac ttgggcttta gtagtagcta actgcactaa
     2341 atataatata aggagggttt tatggggaaa ggccattgga atcggaaaag agtttatagc
     2401 attcgtaagt ttgctgtggg agcttgctca gtaatgattg ggacttgtgc agttctatta
     2461 ggaggaaata tagctggaga atctgtagtt tatgcggatg aaacacttat tactcatact
     2521 gctgagaaac ctaaagagga aaaaatgata gtagaagaaa aggctgataa agctttggaa
     2581 actaaaaatg tagttgaaag gacagaacaa agtgaaccta gttcaactga ggctattgca
     2641 tctgagaaga aagaagatga agccgtaact ccaaaagagg aaaaagtgtc tgctaaaccg
     2701 gaagaaaaag ctccaaggat agaatcacaa gcttcaagtc aagaaaaacc gctcaaggaa
     2761 gatgctaaag ctgtaacaaa tgaagaagtg aatcaaatga ttgaagacag gaaagtggat
     2821 tttaatcaaa attggtactt taaactcaat gcaaattcta aggaagccat taaacctgat
     2881 gcagacgtat ctacgtggaa aaaattagat ttaccgtatg actggagtat ctttaacgat
     2941 ttcgatcatg aatctcctgc acaaaatgaa ggtggacagc tcaacggtgg ggaagcttgg
     3001 tatcgcaaga ctttcaaact agatgaaaaa gacctcaaga aaaatgttcg ccttactttt
     3061 gatggcgtct atatggattc tcaagtttat gtcaatggtc agttagtggg gcattatcca
     3121 aatggttata accagttctc atacgatatc accaaatacc ttcacaaaga tggtcgtgag
     3181 aatgtgattg ctgtccatgc agtcaacaaa cagccaagta gccgttggta ttcaggaagt
     3241 ggtatctatc gtgatgtgac tttacaagtg acagataagg tgcatgttga gaaaaatggg
     3301 acaactattt taacaccaaa acttgaagaa caacaacatg gcaaggttga aactcatgtg
     3361 accagcaaaa tcgtcaatac ggacgacaaa gaccatgaac ttgtagccga atatcaaatc
     3421 gttgaacgag gtggtcatgc tgtaacaggc ttagttcgta cagcgagtcg taccttaaaa
     3481 gcacatgaat caacaagcct agatgcgatt ttagaagttg aaagaccaaa actctggacc
     3541 gttttaaatg acaaacctgc cttgtacgaa ttgattacgc gtgtttaccg tgacggtcaa
     3601 ttggttgatg ctaagaagga tttgtttggt taccgttact atcactggac tccaaatgaa
     3661 ggtttctctt tgaatggtga acgtattaaa ttccatggag tatccttgca ccacgaccat
     3721 ggggcgcttg gagcagaaga aaactataaa gcagaatatc gccgtctcaa acaaatgaag
     3781 gagatgggag ttaactccat ccgtacaacc cacaaccctg ctagtgagca aaccttgcaa
     3841 atcgcagcag aactaggttt actcgttcag gaagaggcct ttgatacgtg gtatggtggc
     3901 aagaaacctt atgactatgg acgtttcttt gaaaaagatg ccactcaccc agaagctcga
     3961 aaaggtgaaa aatggtctga ttttgaccta cgtaccatgg tcgaaagagg caaaaacaac
     4021 cctgctatct tcatgtggtc aattggtaat gaaataggtg aagctaatgg tgatgcccac
     4081 tctttagcaa ctgttaaacg tttggttaag gttatcaagg atgttgataa gactcgctat
     4141 gttaccatgg gagcagataa attccgtttc ggtaatggta gcggagggca tgagaaaatt
     4201 gctgatgaac tcgatgctgt tggatttaac tattctgaag ataattacaa agcccttaga
     4261 gctaagcatc caaaatggtt gatttatgga tcagaaacat cttcagctac ccgtacacgt
     4321 ggaagttact atcgccctga acgtgaattg aaacatagca atggacctga gcgtaattat
     4381 gaacagtcag attatggaaa tgatcgtgtg ggttggggga aaacagcaac cgcttcatgg
     4441 acttttgacc gtgacaacgc tggctatgct ggacagttta tctggacagg tacggactat
     4501 attggtgaac ctacaccatg gcacaaccaa aatcaaactc ctgttaagag ctcttacttt
     4561 ggtatcgtag atacagccgg cattccaaaa catgacttct atctctacca aagccaatgg
     4621 gtttctgtta agaagaaacc gatggtacac cttcttcctc actggaactg ggaaaacaaa
     4681 gaattagcat ccaaagtagc tgactcagaa ggtaagattc cagttcgtgc ttattcgaat
     4741 gcttctagtg tagaattgtt cttgaatgga aaatctcttg gtcttaagac tttcaataaa
     4801 aaacaaacca gcgatgggcg gacttaccaa gaaggtgcaa atgctaatga actttatctt
     4861 gaatggaaag ttgcctatca accaggtacc ttggaagcaa ttgctcgtga tgaatctggc
     4921 aaggaaattg ctcgagataa gattacgact gctggtaagc cagcggcagt tcgtcttatt
     4981 aaggaagacc atgcgattgc agcagatgga aaagacttga cttacatcta ctatgaaatt
     5041 gttgacagcc atgggaatgt ggttccaact gctaataatc tggttcgctt ccaattgcat
     5101 ggccaaggtc aactggtcgg tgtagataac ggagaacaag ccagtcgtga acgctataag
     5161 gcgcaagcag atggttcttg gattcgtaaa gcatttaatg gtaaaggtgt tgccattgtc
     5221 aaatcaactg aacaagcagg gaaattcacc cttactgccc actctgatct cttgaaatcg
     5281 aaccaagtca ctgtctttac tggtaagaaa gaaggacaag agaagactgt tttggggaca
     5341 gaagtgccaa aagtacagac cattattgga gaggcacctg aaatgcctac cactgttccg
     5401 tttgtataca gtgatggtag ccgtgcagaa cgtcctgtaa cctggtcttc agtagatgtg
     5461 agcaagcctg gtattgtaac ggtgaaaggt atggctgacg gacgagaagt agaagctcgt
     5521 gtagaagtga ttgctcttaa atcagagcta ccagttgtga aacgtattgc tccaaatact
     5581 gacttgaatt ctgtagacaa atctgtttcc tatgttttga ctgatggaag tgttgaagag
     5641 tatgaagtgg acaagtggga gattgccgaa gaagataaag ctaagttagc aattccaggt
     5701 tctcgtattc aagcgaccgg ttatttagaa ggtcaaccaa ttcatgcaac ccttgtggta
     5761 gaagaaggca atcctgcggc acctgcagta ccaactgtaa cggttggtgg tgaagctgtc
     5821 acaggtctta ctagtcaaaa accaatgcaa taccgcactc ttgcttatgg agctaagttg
     5881 ccagaagtca cagcaagtgc taaaaatgca gctgttacag ttcttcaagc aagcgcagca
     5941 aacggcatgc gtgcgagcat ctttattcag cctaaagatg gtggccctct tcaaacctat
     6001 gcaattcaat tccttgaaga agcgccaaaa attgctcact tgagcttgca agtggaaaaa
     6061 gctgacagtc tcaaagaaga ccaaactgtc aaattgtcgg ttcgagctca ctatcaagat
     6121 ggaacgcaag ctgtattacc agctgataaa gtaaccttct ctacaagtgg tgaaggggaa
     6181 gtcgcaattc gtaaaggaat gcttgagttg cataagccag gagcagtcac tttgaacgct
     6241 gaatatgagg gagctaaagg ccaagttgaa ctcactatcc aagccaatac tgagaagaag
     6301 attgcgcaat ccatccgtcc tgtaaatgta gtgacagatt tgcatcagga accaagtctt
     6361 ccagcaacag taacagttga gtatgacaaa ggtttcccta aaactcataa agtcacttgg
     6421 caagctattc cgaaagaaaa actagactcc tatcaaacat ttgaagtact aggtaaagtt
     6481 gaaggaattg accttgaagc gcgtgcaaaa gtctctgtag aaggtatcgt ttcagttgaa
     6541 gaagtcagtg tgacaactcc aatcgcagaa gcaccacaat taccagaaag tgttcggaca
     6601 tatgattcaa atggtcacgt ttcatcagct aaggttgcat gggatgcgat tcgtccagag
     6661 caatacgcta aggaaggtgt ctttacagtt aatggtcgct tagaaggtac tcaattaaca
     6721 actaaacttc atgttcgcgt atctgctcaa actgagcaag gtgcaaacat ttctgaccaa
     6781 tggaccggtt cagaattgcc acttgccttt gcttcagatt caaatccaag cgacccagtt
     6841 tcaaatgtta atgacaagct catttcctac aataaccaac cagccaatcg ttggacaaac
     6901 tggaatcgta gtaatccaga agcttcagtc ggtgtcctat tcggagattc aggtattttg
     6961 agcaaacgtt cagttgataa cttaagcgtt ggcttccatg aagaccatgg agttggtgca
     7021 ccgaagtctt atgtgattga gtattatgtt ggtaagactg tcccaacagc tcctaaaaac
     7081 cctagttttg ttggtaatga ggaccatgtc tttaatgatt ctgccaactg gaaaccagtt
     7141 actaatctaa aagcccctgc tcaactcaag gctggagaaa tgaaccactt tagctttgat
     7201 aaagttgaaa cctatgctgt tcgtattcgc atggtgagag cagacaacaa actaggaacg
     7261 tctatcacag aggtacaaat ctttgcgaaa caagttgcgg cagccaagca aggacaaaca
     7321 agaatccaag ttgacggtaa agacttagca aacttcaacc ctgatctgac agactactac
     7381 cttgagtctg tagatggaaa agttccggca gtcacagcaa gtgttagcaa caatggtctc
     7441 gctaccgtcg ttccaagcgt tcgtgaaggt gagccagttc gtgtcatcgc gaaagctgaa
     7501 aatggcgaca tcttaggaga ataccgtctg cacttcacta aggataagaa cttactttct
     7561 cataaaccag ttgctgcggt taaacaagct cgcttgctac aagtaggtca agcacttgaa
     7621 ttgccgacta aggttccagt ttacttcaca ggtaaagacg gctacgaaac aaaagatttg
     7681 tcagttgaat gggaagaagt tccagcagaa aatctgacaa aagcaggtca atttaccgtt
     7741 cgaggccatg tccttggtag tgatcttgtt gctgaggtca ctgtacgagt gacagacaaa
     7801 ctaggcgaag ctctttcaga taaccctaac tatgatgaaa acagtaacca ggcctttgct
     7861 tcagcaacca atgatattga caaaaactct catgaccgcg ttgactatct caatgacgga
     7921 gatcattcag aaaatcgtcg ttggacaaac tggtctccaa caccatcttc taatccagaa
     7981 gtatcagcgg gtgtgatctt ccgtgaaaat ggtaagattg tagaacggac tgttgcgcaa
     8041 gccaaacttc acttctttgc agatagtggt acggatgcac caactaaact cgttttagaa
     8101 cgctatgtcg gtccagagtt tgaagtgcca acctactatt caaactacca agcctacgac
     8161 gcagaccatc cattcaacaa tccagaaaat tgggaagctg tgccttatcg tgcggataaa
     8221 gacatcgcag ctggtgatga aatcaacgta acatttaaag ctgtcaaagc caaagctatg
     8281 agatggcgta tggagcgtaa agcagataag agcggtgttg cgatgattga gatgaccttc
     8341 cttgcaccaa gtgaattgcc tcaagaaagc acgcaatcga agattcttgt agatggaaaa
     8401 gaacttgctg atttcgctga aaatcgtcaa gactatcaaa ttacctataa aggtcaacgg
     8461 ccaaaagtct cagttgaaga aaacaatcaa gtagcttcaa ctgtggtaga tagtggagaa
     8521 gatagccttc cagtacttgt tcgcctcgtt tcagaaagtg gaaaacaagt caaggaatac
     8581 cgtatccact tgactaagga aaaaccagtt tctgagaaga cagttgctgc tgtacaagaa
     8641 gatcttccaa aactcgaatt tgttgaaaaa gatttggcct acaagacagt tgagaaaaaa
     8701 gattcaacac tgtatctagg tgaaactcgt gtagaacaag aaggaaaaac tggtaaagaa
     8761 cgtatcttta cagcgattaa tcctgatgga agtaaggaag aaaaactccg tgaagtggta
     8821 gaagctccga cagaccgcat cgtcttggtt ggaaccaaac cagtagctca agaagctaaa
     8881 aaaccacaag tgtcagaaaa agcagataca aaaccaattg attcaagtga agctagtcaa
     8941 actaataaag cccagttacc aaatacaggt agtgcggcaa gccaagcagc agtagcagca
     9001 ggtttagctc ttctaggttt gagtgcagga ttagtagtta ctaaaggtaa aaaagaagac
     9061 tagaaatttt gataccttct ttatcattaa agaccaaaga aaaactcttg tcctaaatag
     9121 aagataaaga gtctggaaca aaatagttct caaccacaaa aaagctagat atttccaatt
     9181 gcggaactct agctttttaa ttttgagtcg ttctcttatt gtatagtgga ttgaaactag
     9241 aatagtacac ttctgcttct aaaacattgt tagaaatcga tttgactgtc cttatcgatt
     9301 tgtcctgttc ttatttcatt tcactatatt ttaggaggtt attggccata agtaccaatc
     9361 ccatgtcaat tctcacttga cacttccctc tcagattaca tctcttgtaa cccaaagaag
     9421 cctttatctg cccaaagaca ggttccacat caatcttgcg ttgagcgaaa atctgtctac
     9481 cttggggaga taaaagcgcc tgacattctt tagctttcaa gttttgatag cgttcgttca
     9541 tatacagtcc cttttgaggg gctgattcag gttcgtcggc gtagtaaacc ttgatttctt
     9601 gttgaaagtc tgtctgtgtt ttctgatatt tggtatggtg aaaacgatag caccagccat
     9661 caggatgtgt gtagctatcc tccttgtcat tatagtgcca attcgctaag tttctagctg
     9721 actgtttata gcctctcttt tgttccttat caaacatggc atatttaatc agaaggggag
     9781 tgatttcttc ttgaaaatag acttgtatct gttcttgaag tttggcggag aatttctctg
     9841 tcgttttctt ccacacaaaa ctatacttgt tggcattggc ttcaatctta gttccgtcaa
     9901 taaacaaaca atctaaggtc actaactctt ccatttttca atttttcgac cagagaaaat
     9961 cccttgtgaa taggcaaata gaagagtaga tacaagcatt ttagggtgat aagacgggcg
    10021 atcaaaggca tgatagaagg cgtggaagtg acaatcctcc aaggtattca ccactttttc
    10081 aatagtaaaa acgagatgat cttgtggcaa gaaggaactg atttctagtg gtaaagttgt
    10141 ttgatttgtg ttatagtgaa tatgcatggg atagcctttc taaatggttt attaagcaat
    10201 tctattttac caagactatc gtaaccatgc aaaaaagcaa cggcaaatcc gttgcctttt
    10261 ttggatataa gagactattg tcccagactc ttttctttgt ataaaattgt atagtgttat
    10321 tgtctatact attttgtaga gaaagcaaaa atccctagaa aatgcttagg gattcgacta
    10381 tttcattaag aacaattcaa caatcatttc tatatacatg ataagggtta ataagaatcc
    10441 tgcacgccag aagaatttga tgaatttagg atagtaaaag ctgcgttttt tcaaaaggaa
    10501 gaaaaagacg agaataatgg ctaggaacga gagggctagc cccagtctag ggaggaaatt
    10561 atgggtaaag gttttagctg taatcaagta atactcaaat accaagactg gaaaagccaa
    10621 atccgcaaag tttcgtccta gtttttttaa tctaaaaagt ttggttagga taatgcagac
    10681 gactaaggtc agtatcaata ataaaataga tgctaatttc attaaaatca tacccatatt
    10741 gtatcataaa aaaaggctaa tggaaacgag aaacagagga atttatagga aagtcaggct
    10801 tttgagattg tgggtgattt ttgttataat gaaagttata aaatcttata ataccttgaa
    10861 tgattattat cgaaaactct ttggagaaaa gacttttaaa gtccctattg atgcgggatt
    10921 tgactgtccc aatcgtgatg gaactgtggc tcatggaggc tgtacttttt gtacggtttc
    10981 cggttctgga gatgccattg tggcaccaga tgcgcctatc cgtgagtaat tttataagga
    11041 aattgacttt attcaccgta agtggccaga tgttcagaag tatctggttt attttcaaaa
    11101 ttttaccaac acccgtgaaa aggtggaagt tattcgagag cgttatgaac aggctatcaa
    11161 tgagccaggt gtggtaggaa tcaatattgg aacgcgccca gactgtttac cagacgaaac
    11221 catcgaatat ttggctgagt tatcggagtg catgcatgtg acggttgaat tgggcttgta
    11281 gactacttat gaagcaactt ctgacctgat taaccgtgcg tattcttatg aattgtaggt
    11341 ggaaacggtc aagcgtttga gaaagtatcc caagattgag attgtttccc atttgatcaa
    11401 tggtttgcct ggtgaaaccc atgagatgat ggttgaaaat gtccgccgtt gtgtcacgaa
    11461 taacgatatt caagggatta aactgcattt gctccatctt atgacaaata ctcgtatgca
    11521 acgagattac catgagggac gtttacaatt gatgagtcag gacgaatatg tcagggtcat
    11581 ctgtgaccaa ctggaaatta ttcccaaaca tatcgtcatc catcgaatta caggagatgc
    11641 gcctagggat atgctgcttg ggcctatgtg gagcctcaat aaatgggaag ctctcaacag
    11701 cattgagatg gagatgcgac gtcgtggaag tgttcaagga tgcaaggctg taaaacagga
    11761 gtttgaaaat gaaaagacca cttgagatgg cacatgattt tttggctgag gtcgtgacaa
    11821 aagaggatgt cgtagtggat gcgactatgg gaaatggtca tgacacgctt tttttagcca
    11881 agctagccaa gcaagtctat gcctttgata ttcagaagca agccttggaa aagacccaag
    11941 agcgtttgca tcaggctgac ttgacaaatg cccagttaat cttgcaaggc catgagacac
    12001 tggaccagtt tgtgataaaa gctaaggcag ggatttttaa tctgggctat ttgccgtcag
    12061 ctgataagtc tgtcatcacc cgaccgcaga caacgattga ggcattagaa aagctatgtg
    12121 gcttacttgt caaaggtgga cgaattgcta ttatgattta ctatggtcat gaaggaggcg
    12181 atctcgagag agatgctgtc ttggattttg tgatccagtt gaaccaacaa gagtacacag
    12241 ctgccattta ccgaacttta aaccaagtca acaacccgcc gtttttagtg atgattgaaa
    12301 aattagagag atacagacat ggataaacaa tacctacacg aaaagctgga tgccatgcgc
    12361 cagaattttg ttgaatcaac ccaccacgaa cgagcggtgg gcgtgctaga ccaagcgcat
    12421 atgagcaaaa aaatgcttaa aatcaagaaa aaattagttg ctcttgaaat ggaacggtgc
    12481 cagagaaaaa ttgagcacaa agactgttcc aagattgacc aaaaaatcaa agagcagaag
    12541 gagatatttg aatcctgttg taaaaaagat taaggaggac gtgcgtggaa ttactgattt
    12601 acctcatcct atttttactg gttttgattg tctcaagtac aaccaataag ctcctgccct
    12661 ttttgcctct ccctttggtg cagattcttt taggaattgt gattggtctc tttttaccca
    12721 atactgactt tcaccttaat acggagttgt ttttggcact ggttatcgga cccttgcttt
    12781 tccgagaggc tgaagaagca gatgttacgg ctattttaaa acactggcga atcattgttt
    12841 atctcatatt tccagtgatt tttatctcga ccctgagttt gggtggcttg gcccatcttc
    12901 tttggttcag ccttcccttg gcagcttgct tggctgttgg tgcagccctt ggtcctacgg
    12961 acttggtggc ctttgcctct ctttcggagc gttttagctt tcctaagcgc gtgtccaata
    13021 ttcttaaggg cgaaggactc ttgaatgatg cttctggttt ggtggctttt caggtagctt
    13081 tgacagcttg gacaactgga gctttttctc tggggcaagc tagcagttcg ctcatctttt
    13141 caatcctagg cggtttttta attggatttt taacagccat gaccaaccgc ttcctccata
    13201 ccttcttgct aagtgtgcgc gcaacggata ttgccagtga acttttatta gaattgagtt
    13261 tgcctctagt gaccttcttt ctggcagaag aagtccatgt ttcagggatt attgccgtcg
    13321 tagttgctgg aattttaaag gcaagtcgct tcaagaaaat cacgctcctc gaagcccaag
    13381 tggatacggt gaccgagacg gtctggcata cagtgacctt tatgctcaac ggttctgtct
    13441 ttgtgatttt agggatggag ttggaaatga tagcagaacc tatcttgacc aatccaatct
    13501 ataatcctct acttttattg ctatctctca tcgcccttac ctttgtcctc tttgtcattc
    13561 gttttattat gatctatggc tattatgcct atagaacccg acgcctcaag aaaaagctaa
    13621 ataagtatat gaaggacatg tttctcttga ccttttcagg tgttaaggga acggtgtcga
    13681 ttgctacgat tctcttgata ccaagtaatc tagaacagga gtatcctctc ttgcttttcc
    13741 ttgttgcagg tgtgacgctt gtcagctttt taacaggtct cttggtcttg cctcatcttt
    13801 ctgatgaaga ggaagaaagc aaggattatc tcatgcatat cgccattttg aatgaagtaa
    13861 cgctagagtt ggaaaaagag ttggaagaca ccagaaataa acttcccctc tatgcggcta
    13921 ttgacaatta tcatggacgt attgaaaatc tcattttaag ccaagaaaac caggatgatc
    13981 aagaagattg ggctgctttg aaactcttga ttcttagtat tgaaagtgat ggtttggagc
    14041 aggcctatga agaggggaac attagcaatc gtgtttaccg agtttaccaa cgttatctga
    14101 aaaatataga acaaggaatc aatcgtaaac ttgcctcaag actgacctat tatttccttg
    14161 tttctttgcg gattttacgt tttctccttc atgaagtttt tactctcgga aagaccttcc
    14221 gtagctggaa gaacaaggag caaagccgtc tccgtgctct tgattatgac caaattgcag
    14281 agctctatct tgccaatacg gagatgatta ttgaaagttt ggaaaacctg aagggagtct
    14341 acagccgctc tttgattagt tttatgcagg agtctcgtct tcgagaaaca actattatta
    14401 gcagtggtgc ctttgtcgaa cgggttatca atcgtgtcaa acccaacaat atcaatgaaa
    14461 tgctgagagg ctattatctg gagcgcaagt tgattttcga atacgaagaa aaacgattga
    14521 ttacgactaa gtatgccaag aaattacgac aaaatgtaaa taacttagag aactattcct
    14581 tgaaggaagc tgccaatacc ctgccgtatg atatggtgga attggtaaga agaaattagt
    14641 taatattctt cgaaaatctc ttcaaaccac gtcagcgtcg ccttaccgta tgtatgtgac
    14701 tgacttcgtc agtcttatct acaacctcaa aacagtgttt tgagcagctt gcggctagct
    14761 ttctagtttg ctctttgatt ttcattgagt ataagattat aaatgaagga gtatgacatg
    14821 aaaaagtggt ggaaagagct gatagacaag cctttattaa aagctttttt gcattattat
    14881 caagcatcag atagtgagtt gaccagtgtc gcagtagcct actattggtt gatttcgatt
    14941 ttccccctgc ttttggtggt ggtcaatatc ctcccctatt ttcagattcc tgtgggagaa
    15001 tttttgggct ttatgaaaga cgttctgccc ccaagcctct atgaaggtgt ggaaaaaata
    15061 gccagagaag ttttgacgca accttcaaca ggtttattga gtttttctgt tttatcggcg
    15121 ttatggagtt tttcaaaatc tatgaatttc ttgcaaaaag cttttaacaa ggcttatggc
    15181 gtcgaaaaga gtcgtggact tatttcccat cagatgctaa gtctcctagt cagttttggc
    15241 ttgcagatcc tctttgcgtt tgccttgttt ttgatcttat ttggtcagat gattttggct
    15301 ttacttgctc attattggac aaaagatggc attatctatc aggctttaca agggttggca
    15361 ggtcctctga tttacgccct cctctttgct atcttggtca tgctttatta ttttcttccc
    15421 aatctatcta acaggaaaat tagctatacc ctaccaggca gtgcctttgt tctcttagtg
    15481 attttaggtt tactaactct gttctctagt tatctcaatt actatgtcca tcatttggtg
    15541 gatgtccgaa ttttaggttc cgttcttctt gttgttatga tgttttggtt tatcttgatt
    15601 gctaaaattg ttatcctagg cgcggttatc aatgccagta tgcagagttt gaaagatccg
    15661 gtctttaaaa aagactaact atttatccat tacaaaagcg catagtatca agactttata
    15721 gaaccttgat actatgcgct tttgtgcttt gacagttttt tgtatagttt ttgtccaaga
    15781 tttctaaatc atctgattaa attcctattt tcccctatca ttgtccctgt tttttcgaaa
    15841 atggaaggag tataataaat ctatcaataa aatgatagga ggaactttat gggtcatatt
    15901 ttcttttttc taagtgtctt tttggcaggg attctatcct tcttttctcc ttgtatctta
    15961 cctttgttac cggtctatac aggagtgtta ctagatgata aggatggtgc tcaggcttct
    16021 agcggcaaat tttcaatctc agttactagt ttattacgaa ctctggcctt tatagcagga
    16081 atttccttta tatttatttt gttgggctat ggagctggtt ttttaggcga tttgctttat
    16141 gcttcttggt tccaatatct tactggggca attattatcc ttcttggttt gcaccaaatg
    16201 gagattctac actttaagga gctttataag gaaaagaggc tacaactgca aggacagggg
    16261 caaaatggta agggctatag tcaggcattt ttattgggct tgacctttag ttttgcttgg
    16321 acgccttgcg tggggccggt tctggggtct gttttggcct tggcggcttc aggtggttca
    16381 ggagcttggc agggagctgg tctcatgttg gtgtatacgc tgggcttggc gctaccattc
    16441 ttgcttctag ctctgacctc tagttatgtt ttgaaacatt tccgaaaact tcatccctat
    16501 ctcggaatcc tcaaaaaagt gggtggtttt ctcattattg tgatgggact cttggttctg
    16561 tttggaaatg cttcaatttt aagtcaatta tttgaataaa atggaaagga atatcaatat
    16621 gaaaaaatgg caaacatgtg ttcttggagc aggttcgctc ctttgtttga cggcttgttc
    16681 aggcaagtcc gtgactagtg aacaccaaac gaaagatgaa atgaagacgg agcagacagc
    16741 tagtaaaaca agcgcagcta aagggaaaga ggtggctgat tttgaattga tgggagtaga
    16801 tggcaagacc taccgtttat ctgattacaa gggcaagaaa gtctatctca aattctgggc
    16861 ttcttggtgt tccatctgtc tggctagtct tccagatacg gatgagattg ctaaagaagc
    16921 tggtgatgac tatgtggtct tgacagtagt gtcaccagga cataagggag agcaatctga
    16981 agcggacttt aagaattggt ataagggatt ggattataaa aatctcccag tcctagttga
    17041 cccatcaggc aaacttttgg aaacttatgg tgtccgttct tacccaaccc aagcctttat
    17101 agacaaagaa ggcaagctgg tcaaaacaca tccaggattc atggaaaaag atgcaatttt
    17161 gcaaactttg aaggaattag cctaggaggt gtcttatgaa tgataagtta aaaatcttct
    17221 tgttgctagg agtatttttt ctagccataa ccggtttcta tgttctattg atacgaaatg
    17281 cagggcagac agatgcctcg caaattgaaa aggcggcagt tagccaagga ggaaaagcag
    17341 tgaaaaaaac agaaattagt aaagacgcag acttgcacga aatttatcta gctggaggtt
    17401 gtttctgggg agtggaggaa tatttctcac gcgttcccgg ggtgacggat gccgtttcag
    17461 gctatgcaaa tggtagagga gaaacaacca agtacgaatt gattaaccaa acaggtcatg
    17521 cagaaaccgt ccatgtcacc tatgatgcca agcaaatttc tctcaaggaa atcctgcttc
    17581 actatttccg cattatcaat ccaactagca aaaataaaca aggaaatgat gtggggaccc
    17641 agtaccgtac tggtgtttat tacacagatg acaaggattt ggaagtgatt aaccaagtct
    17701 ttgatgaggt ggctaagaaa tacgatcaac ctctagcagt tgaaaaggaa aacttgaaga
    17761 attttgtggt ggctgaggat taccatcaag actatctcaa gaaaaatcca aatggctact
    17821 gccatatcaa tgttaatcag gcggcctatc ctgtcattga tgccagcaaa tatccaaaac
    17881 caagtgatga ggaattgaaa aagaccctgt cacctgagga gtatgcagtt acccaggaaa
    17941 atcaaacaga acgagctttc tcaaaccgtt actgggataa atttgaatcc ggtatctatg
    18001 tggatatagc aactggggaa cctctctttt catcaaaaga caaatttgag tctggttgtg
    18061 gctggcctag ttttacccaa cccatcagtc cagatgttgt cacctacaag gaagataagt
    18121 cctacaatat gacgcgtatg gaagtgcgga gccgagtagg agattctcac cttgggcatg
    18181 tctttacgga tggtccacag gacaagggcg gcttacgtta ctgtatcaat agcctctcta
    18241 tccgctttat tcccaaagac caaatggaag aaaaaggcta cgcttattta ctagattatg
    18301 ttgattaata ctcttcgaaa atctcttcaa accacgtcag cgtcgcctta ccgtatgtag
    18361 ggttactgac ttcgtcagtt ttatctacaa cctcaaagct atactttgag ctgacttcgt
    18421 cagttttatt tacaacctca aagctatact ttgagaaacc tgtggctatc ttcctagttt
    18481 actttttgat ttttattgag tataagaggt ctttcctaag cagttagagg agaattttgc
    18541 tatactgata ctagtaagtg gtaaaggagc agatcatgac ctacacaatc ttaatcgtag
    18601 aagatgaata tctggtaaga caaggtttga ctaaactggt caatgtagca gcctacgata
    18661 tggaaatcat tggtcaggct gaaaatggaa ggcaggcttg ggaattgatc caaaagcagg
    18721 tgccagatat cattttaacc gatatcaaca tgcctcatct aaatggcatc cagttggcca
    18781 gtctggtacg agaaacctat cctcaggttc atttggtctt tttaacaggt tacgatgatt
    18841 ttgattatgc cttgtctgct gtcaaactag gtgtggacga ctacctgctc aaaccctttt
    18901 ctcgtcagga tattgaggaa atgttgggga aaatcaaaca aaaactagac aaggaagaga
    18961 aagaagagca gttacaagat ttattgacta ataggtttga aggaaacatg gcccagaaaa
    19021 tccagtctca tctggctgat agccaattta gtttaaagtc tttagccagt gacttaggtt
    19081 ttagtccgac ctatctgagt tccttgatta agaaagagtt gggcttgcct tttcaggatt
    19141 atctggtgag agaacgtgtt aaacaagcca agctcttgct tttaactaca gatctgaaga
    19201 tttatgagat cgcagagaag gttggttttg aagatatgaa ctattttacc caacgtttta
    19261 agcagattgc aggtgtgaca cctcgtcagt ttaagaaggg agaagaccga tgaagcgttc
    19321 ttctctttta gttagaatgg ttatttccat ctttctggtc tttctcattc tcctagctct
    19381 ggttggaact ttctactatc aatcaagttc ttcagccatt gaggccacca ttgagggcaa
    19441 cagccaaacg accatcagcc agactagcca ctttattcag tcttatatca aaaaactaga
    19501 aaccacttcg accggtttga cccagcagac ggatgttctg gcctatgctg agaatcccag
    19561 tcaagacaag gtcgagggaa tccgagattt gtttttgacc atcttgaagt cagataagga
    19621 cttgaaaact gttgtgctgg tgaccaaatc tggtcaggtc atttctacag atgacagtgt
    19681 gcagatgaaa acttcctctg atatgatggc tgaggattgg taccaaaagg ccattcatca
    19741 gggagctatg cctgttttga ctccagctcg taaatcagat agtcagtggg tcatttctgt
    19801 cactcaagaa cttgttgatg caaagggagc caatcttggt gtgcttcgtt tggatatttc
    19861 ttatgaaact ctggaagcct atctcaatca actccagttg gggcagcagg gctttgcctt
    19921 cattatcaat gaaaaccatg aatttgtcta ccatcctcaa cacacagttt atagttcgtc
    19981 tagcaaaatg gaggctatga aaccctacat cgatacaggt cagggttata ctcctggtca
    20041 caaatcctac gtcagtcaag agaagattgc aggaactgat tggacggtac ttggcgtgtc
    20101 atcattggaa aagttagacc aggttcggag tcagctcttg tggaccttgc ttggggccag
    20161 tgtcacatct cttcttgtct gtctctgctt agtgtggttc agtcttaaac gctggattgc
    20221 tcctttgaag gatttgagag aaaccatgtt ggaaattgct tctggtgctc aaaatcttcg
    20281 tgccaaggaa gttggtgcct atgaactgag agaagtaact cgccaattta atgccatgtt
    20341 ggatcagatt gatcagttga tggtagctat tcgtagccag gaagaaacga cccgtcagta
    20401 ccaacttcaa gccctttcga gccagattaa tccacatttc ctctataaca ctttggacac
    20461 catcatctgg atggctgaat ttcatgatag tcagcgagtg gtgcaggtga ccaagtcctt
    20521 ggcaacctat ttccgcttgg cgctcaatca aggcaaggac ttgatttgtc tctctgacga
    20581 aatcaatcat gtccgccagt atctctttat ccagaaacaa cgctatggag ataagctgga
    20641 atacgaaatt aatgaaaatg ttacctttga taatttagtc ttacccaagc tggtcctaca
    20701 accccttgta gaaaatgctc tttaccatgg cattaaggaa aaggaaggtc agggccatat
    20761 taaactttct gtccagaaac aggattcggg attggtcatc cgtattgagg atgatggcgt
    20821 tggcttccaa gatgctggtg atagtagtca aagtcaactc aaacgtgggg gagttggtct
    20881 tcaaaatgtc gatcaacggc tcaaacttca ttttggagcc aattaccaga tgaagattga
    20941 ttctagaccc caaaaaggga cgaaagttga aatatatata aatagaatag aaactagcta
    21001 actcccagtc aattctggga gttttatgcg taattgggca aactttctag gttgtctatc
    21061 ttgctaaaaa caagaaaaaa cggtatgata aaagagtgac aacagaggta acagagatga
    21121 acgagacaca gattcaaaga gaaacacgtc aggtagtgga agatgtttta gaaaagacca
    21181 atttgaagca gggggccctt tttgttttag gtctatcttc tagtgaggtg ctaggtggtc
    21241 agattgggaa agaatccagt caagagattg gtgagctgat agtggagact atcttgggta
    21301 ttctaagtag tagaggtatc catttagcag ttcaaggttg tgagcatgtc aatcgggctc
    21361 tcgttgttga acgtcaggtg gcagagcagt ttggtctgga aattgtcagt gtccatccta
    21421 ctcttcatgc aggaggttcg gggcagttgg cagcctttaa gtttatgcag gatccagttg
    21481 aggttgaatt tatcaaggct catgctggat tggatatcgg agacactgca attggcatgc
    21541 atgtcaagca tgttcaggtt ccgattcgcc ctattttgag agagattggt catgcccatg
    21601 taacggcact ggctagtcgt ccaaaattaa tcggaggtgc gcgtgcgcac tatccgcaag
    21661 acgctattag aaagtcttga gaatgaataa aggagataaa agtgtttaaa aaagaccgtt
    21721 tttcaattcg taagattaag ggagttgtag gctctgtatt tcttggaagc cttttgatgg
    21781 ctccttctgt agtggacgca gccacctatc actatgtaaa taaagagatt atttcacaag
    21841 aagctaaaga tttaattcag acaggaaagc ctgacaggaa tgaagttgta tatggtttgg
    21901 tgtatcaaaa agatcagttg cctcaaacag ggacagaagc atctgttttg acagcttttg
    21961 gtttgctgac tgttgggagc ttgcttttaa tctacaagag aaagaaaatt gctagcgtct
    22021 ttctagttgg agctatggga ttggtagttc ttcctagtgc aggggctgta gacccagttg
    22081 cgaccctagc gctggctagt cgagagggtg ttgttgaaat ggagggctat cgctatgttg
    22141 gttatctatc aggtgacatc ctcaaaacgc ttggcttgga cactgtttta gaagaaacct
    22201 cagcaaaacc tggagaggtg actgtagtcg aagttgagac tcctcaatca acaacaaatc
    22261 aggagcaagc taggacagaa aaccaagtag tagagacaga ggaagcacct aaaacagaag
    22321 aaagtccaaa ggaagaacca aaatcggaga taaaacctac tgacgacacc cttcctaaag
    22381 tagaagaggg gaaagaagat tcagcagaac cagctccagt tgaagaagta ggtggagaag
    22441 ttgagtcaaa accagaggaa aaagtagcag ttaagccaga aagtcaacca tcagacaaac
    22501 cagctgagga atcaaaagtt gaaccaccag tagaacaagc aaaaggccca gaacaacccg
    22561 tgcaacctac acaagctgag caaccaagga taccaaaaga ttcatcacaa ccagaagatc
    22621 ctaaagaaga taggggagcg gaagatacac cgaaacaaga agatacaccg aaacaagaag
    22681 atacacagcc agaagtagta gaaacaaaag atgaggctgc taatcaacct gttgaagaac
    22741 caaaagttga aacgcctgct gtagaaaaac aaacggaacc aaaagttgaa caagtaggtg
    22801 aaccagtcga gccaagtgaa gacgaaaagg caccagtctc tccagaaaag caaccagaag
    22861 ctcctgaaga gaaggctgta gaggaaacac cgaaaccaga agataaaata aagggtattg
    22921 gtactaaaga accagttgat aaaagtgagt taaataatca aattgataaa gctagttcag
    22981 tttctcctac tgattattct acagcaagtt acaatgctct tggacctgtt ttagaaactg
    23041 caaaaggtgt ctatgcttca gagcctgtaa aacagcctga ggtaaatagc gagacaaata
    23101 aacttaaaac ggctattgac gctctaaacg ttgataaaag tgaattacag gaacaattaa
    23161 gagttgctga acaaaaacaa caagctgact atagcgctaa aacttggagg gaattcaaaa
    23221 ttgctgaact tcaagctaaa gaaatcaaca accaaactac accacttcct aaacaaagtg
    23281 aaatagatgc agctactaag gctcttcaag acgctcttca agctcttgct gtagataaaa
    23341 ctgtattaca aaacgctatt aacacagcta atagcaaacg tgaagaagag tatactgctc
    23401 agacatggaa agcgttagaa gatgctctta cagcagtgaa tcctgttaat gaagatgaaa
    23461 ctgcaactca aagcaaagta gacgaagcta ctagaaactt agaagaagct atcaataacc
    23521 tagttctatt aactgaaaaa ccggtattaa catttattga gactgataag aaagcattag
    23581 aacgtgaagt agttgctaag tattctctag aaaaccaaaa taaaacaaaa attaaatcaa
    23641 tcacagctac tctgaaaaaa ggagaaacag ttgttagcac tgtagagctt ataggcgatg
    23701 atgtaactaa tgaaactata acctctgcat ttaagaatct agagtactac aaagaataca
    23761 ccctatctac aactatggtt tacgatagag gtgacggtga tgtaacagaa atcttagata
    23821 atcaaccaat ccaactagat cttaaaaaag ttgagcttaa aaatattaaa cgtacagatt
    23881 taatcaaata cgaaaatgga aaagaaacta atgaatcact aataacaact gttcctgatg
    23941 ataagcggaa ttattattta aaaataactt caaagaatca aaaaactaca ttactagctg
    24001 ttaaaaatat agaagaaact acggttaacg gaacacctgt atataaagtt acagcaatcg
    24061 cagacaattt agtctctaga actgctgata ataaatttga agaagaatac gttcactata
    24121 ttgaaaaacc taaagtccac gaagataatg tatattataa cttcaaagaa ttagtggagg
    24181 ctattcaaaa cgatccttca aaagaatatc gtctgggaca atcaatgagc gctagaaatg
    24241 ttgttcctaa tggaaaatca tatatcacta aagaatttac aggaaaactt ttaagttctg
    24301 aaggaaaaca atttgctatc actgagttgg aacatccatt atttaatgtg ataacaaacg
    24361 caacgataaa taatgtgaat tttgaaaatg tagagataga acgttctggt caagataata
    24421 ttgcatcatt agccaatact atgaaaggtt cttcagttat tacaaatgtc aaaattacag
    24481 gcacactttc aggtcgtaat aatgttgctg gatttgtaaa taatatgaat gatggaactc
    24541 gtattgaaaa tgttgctttc tttggcaaac tacactctac aagtggaaat ggctctcata
    24601 cagggggaat tgcaggtaca aactatagag gaattgttag aaaagcatat gttgatgcta
    24661 ctattacagg aaacaaaaca cgcgccagct tgttagttcc taaagtagat tatggattaa
    24721 ctctagacca tcttattggt acaaaagctc tcctaactga gtcggttgta aaaggtaaaa
    24781 tagatgtttc aaatccagta gaagttggag caatagcaag taagacttgg cctgtaggta
    24841 cggtaagtaa ttctgtcagc tatgctaaga ttatccgtgg agaggagtta ttcggctcta
    24901 acgacgttga tgattctgat tatgctagtg ctcatataaa agatttatat gcggtagagg
    24961 gatattcgtc aggtaataga tcatttagga aatctaaaac atttactaaa ttaactaaag
    25021 aacaagctga tgctaaagtt actactttca atattactgc tgataaatta gaaagtgatc
    25081 tatctcctct tgcaaaactt aatgaagaaa aagcctattc tagtattcaa gattataacg
    25141 ctgaatataa ccaagcctat aaaaatcttg aaaaat
//
LOCUS       DAVLVP010000020        22776 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.020, whole genome shotgun sequence.
ACCESSION   DAVLVP010000020 DAVLVP010000000
VERSION     DAVLVP010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22776
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.020"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(40..3216)
                     /gene="carB"
                     /locus_tag="VYN69_000795"
     CDS             complement(40..3216)
                     /gene="carB"
                     /locus_tag="VYN69_000795"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEV1533098.1"
                     /translation="MPKRTDIQKIMVIGSGPIIIGQAAEFDYAGTQACLSLKEEGYEV
                     VLVNSNPATIMTDKEIADKVYIEPITLEFVTRILRKEGPDALLPTLGGQTGLNMAMEL
                     SKNGILDELGVELLGTKLSAIDQAEDRDLFKQLMEELEQPIPESEIVNTVEEAVAFAA
                     TIGYPVIVRPAFTLGGTGGGMCANEKELREITENGLKLSPVTQCLIERSIAGFKEIEY
                     EVMRDSADNALVVCNMENFDPVGIHTGDSIVFAPAQTMSDYENQMLRDASLSIIRALK
                     IEGGCNVQLALDPNSFKYYVIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEV
                     INPVTGSTYAMFEPALDYVVAKIPRFPFDKFEKGERRLGTQMKATGEVMAIGRNIEES
                     LLKACRSLEIGVHHNEIPELAAVSDDALIEKVVKAQDDRLFYVSEAIRRGYTPEEIAE
                     LTKIDIFYLDKLLHIFEIEQELGAHPQDLEVLKTAKLNGFSDRKIAELWGTTDDKVRQ
                     LRLENKIVPVYKMVDTCAAEFDSETPYFYSTYGWENESIRSDKESVLVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQAAGYEAIIMNSNPETVSTDFSVSDKLYFEPLTFEDVMNVIDL
                     EQPKGVIVQFGGQTAINLAEPLAKAGVTILGTQVADLDRAEDRDLFEQALKELDIPQP
                     PGQTATNEEEAALAARKIGFPVLVRPSYVLGGRAMEIVENEEDLRSYMRTAVKASPDH
                     PVLVDSYIVGQECEVDAISDGKNVLIPGIMEHIERAGVHSGDSMAVYPPQTLSQKVQE
                     TIADYTKRLAIGLHCLGMMNIQFVIKDEKVYVIEVNPRASRTVPFLSKVTNIPMAQVA
                     TKLILGQSLSELGYQNGLYPESTRVHIKAPVFSFTKLAKVDSLLGPEMKSTGEVMGSD
                     ATLEKALYKAFEASYLHLPTFGNVVFTIADDAKEEALNLARRFQNIGYGILATEGTAA
                     FFASHGLQAQPVGKIGDDDKDIPSFVRKGRIQAIINTVGTKRTADEDGEQIRRSAIEH
                     GVPLFTALDTANAMLKVLESRSFVTEAI"
     gene            complement(3529..4608)
                     /gene="carA"
                     /locus_tag="VYN69_000796"
     CDS             complement(3529..4608)
                     /gene="carA"
                     /locus_tag="VYN69_000796"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000166701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEV1533099.1"
                     /translation="MTKRILVLEDGTVFEGKAFGADIDVTGEIVFNTGMTGYQESITD
                     QSYNGQILTFTYPLVGNYGINRDDYESIIPTCKGVVVFEEARRASNWRNQMTLDEFLK
                     AKKIPGISGIDTRALTKIIRKHGTMRATLTHVGDSMDHVTDQLQATVLPTDNIKQVST
                     KTSYPAPGVGLSVVLVDFGLKHSILRELSKRNCNVTVVPYSTKAEEILHLNPDGVMLS
                     NGPGNPEDVPQALDMIRGVQGKIPIFGICMGHQLFAMANGAKTYKMKFGHRGFNHAVR
                     EIATGRVDFTSQNHGYAVSREDLPEHLIITHEEINDKSVEGVRHRYQPAFSVQYHPDA
                     APGPHDASYLFDEFIEMMEVFKQSN"
     gene            complement(4658..5581)
                     /locus_tag="VYN69_000797"
     CDS             complement(4658..5581)
                     /locus_tag="VYN69_000797"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001293822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEV1533100.1"
                     /translation="MSENQQALNHVVSMEDLTVDQVMKLIKRGIEFKNGAQLPYEDHP
                     IVSNLFFEDSTRTHKSFEVAEIKLGLERLDFDVKTSSVNKGETLYDTILTLSALGVDV
                     CVIRHPEVDYYRELIASPTITTSIINGGDGSGQHPSQSLLDLMTIYEEFGHFEGLKVA
                     IAGDLDHSRVAKSNMQILKRLGSELFFAGPEEWRSQEFADYGQFVTIDEIIDQVDVMM
                     FLRVQHERHDSGAVFSKEDYHAQHGLTQERYDRLKETAILMHPAPINRDVEIADHLVE
                     APKSRIVQQMTNGVFVRMAILESVLASRNAN"
     gene            complement(5600..6121)
                     /gene="pyrR"
                     /locus_tag="VYN69_000798"
     CDS             complement(5600..6121)
                     /gene="pyrR"
                     /locus_tag="VYN69_000798"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEV1533101.1"
                     /translation="MKTKEVVDELTVKRAITRITYEIIERNKDLNKIVLAGIKTRGVF
                     IAHRIQERLKQLENLSVPVVELDTKPFRDDVKSGEDTSLVSVDVTDREVILVDDVLYT
                     GRTIRAAIDNIVGHGRPARVSLAVLVDRGHRELPIRPDYVGKNIPTSRSEEIIVEMTE
                     LDDQDRVLITEEA"
     gene            complement(6332..6961)
                     /gene="nth"
                     /locus_tag="VYN69_000799"
     CDS             complement(6332..6961)
                     /gene="nth"
                     /locus_tag="VYN69_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEV1533102.1"
                     /translation="MVLSKKRARKVLEEIIALFPDAKPSLDFTNHFELLVAVMLSAQT
                     TDAAVNKATPGLFVAFPTPQAMSVATESEIASHISRLGLYRNKAKFLKKCAQQLLDDF
                     DGQVPQTREELESLAGVGRKTANVVMSVGFGIPAFAVDTHVERICKHHDIVKKSATPL
                     EVEKRVMDILPPEQWLAAHQAMIYFGRAICHPKNPECDQYPQLYDFSNL"
     gene            complement(6961..7503)
                     /locus_tag="VYN69_000800"
     CDS             complement(6961..7503)
                     /locus_tag="VYN69_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEV1533103.1"
                     /translation="MKLNIQEIRKQSEGLNFEQTLDLVDDLRARNQEILDVKDILAVG
                     KVQYEDRMYFLDYQLSYTIVLASSRSMEPVELVESYPVTEVFMEGATNHLNQEILDDD
                     LVLPIENEALDLAESVSDNILLNIPIKVLTAEEEAGQGFVSGNDWQIMTEEEYQAQQA
                     VKKEENSPFAGLQGLFDGDE"
     gene            7673..7990
                     /locus_tag="VYN69_000801"
     CDS             7673..7990
                     /locus_tag="VYN69_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1533104.1"
                     /translation="MEDKKLIQLLSKLNKSYQNCKQGTADDIRLQELLNTTMQELKKT
                     EKLNNSILIDLEKFYQPTSLLIGLGSLKLNDQARTAWRNYDKFHYEHVKHVLSLYGPV
                     FGF"
     gene            8229..9788
                     /locus_tag="VYN69_000802"
     CDS             8229..9788
                     /locus_tag="VYN69_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011681787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1533105.1"
                     /translation="MHYRHSRKGNSMIKINHLTITQNKDLRDLVSDLSMTIQDGEKVA
                     IIGEEGNGKSTLLKTLMGEDLSDFTIKGDIQSDYQSLAYIPQKVPEDLKKKTLHDYFF
                     LDSIDLDYSILYRLAEELHFDSNRFASDQEIGNLSGGEALKIQLIHELAKPFEILFLD
                     EPSNDLDLETVDWLKGQIQKTRQTVIFISHDEDFLSETADTIVHLRLVKHRKEAETLV
                     EHLDYDSYSEQRKANFAKQSQQAANNQRAYDKTMEKHRRVKQSVETALRATKDSTAGR
                     LLAKKMKTVLSQEKRYEKAAQSMTQKPLEEEQIQLFFSDIQPLPASKVLVQLEKENLS
                     IDDRVLVQKLQLTVRGQEKIGIIGPNGVGKSTLLAKLQRLLNDKREISLGFMPQDYHK
                     KLQLDLSPIAYLSKTGEKEELQKIQSHLASLNFSYPEMQHQIRSLSGGQQGKLLLLDL
                     VLRKPNFLLLDEPTRNFSPTSQPQIRKLFATYPGGLITVSHDRRFLKEVCSIIYRMTE
                     HGLKLVNLEDL"
     gene            complement(9937..10836)
                     /gene="htpX"
                     /locus_tag="VYN69_000803"
     CDS             complement(9937..10836)
                     /gene="htpX"
                     /locus_tag="VYN69_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease HtpX"
                     /protein_id="HEV1533106.1"
                     /translation="MLFDQIASNKRKTWILLLVFFLLLALVGYAVGYLFIRSGLGGLV
                     IALIIGFIYALSMIFQSTEIVMSMNGAREVDEQTAPDLYHVVEDMALVAQIPMPRVFI
                     IDDPALNAFATGSNPQNAAVAATSGLLAIMNREELEAVMGHEVSHIRNYDIRISTTAV
                     ALASAITMLSSMAGRMMWWGGAGRRRSDDDRDGNGLEIILLVVSLLAIVLAPLAATLV
                     QLAISRQREFLADASSVELTRNPQGMINALDKLDNSKPMSRHVDDASSALYINDPKKG
                     GGFQKLFYTHPPISERIERLKQM"
     gene            complement(10838..11398)
                     /locus_tag="VYN69_000804"
     CDS             complement(10838..11398)
                     /locus_tag="VYN69_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HEV1533107.1"
                     /translation="MTWIILGVLALVVIFVIVSYNGLVKNRMQTKEAWSQIDVQLKRR
                     NDLLPNLIETVKGYAKYEGSTLEKVAELRNQVAAATSPAEAMKASDALTRQVSGIFAV
                     AESYPDLKASANFVKLQEELTNTENKISYSRQLYNSVVSNYNVKLETFPSNIIAGMFG
                     FKAADFLQTPEEEKSVPKVDFSGLGD"
     gene            11492..12205
                     /gene="rsmG"
                     /locus_tag="VYN69_000805"
     CDS             11492..12205
                     /gene="rsmG"
                     /locus_tag="VYN69_000805"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEV1533108.1"
                     /translation="MKPKTFYNLLAEQNLPLSDQQKEQFERYFELLVEWNEKINLTAI
                     TDKEEVYLKHFYDSIAPILQGLIPNETIKLLDIGAGAGFPSLPMKILYPELDVTIIDS
                     LNKRINFLQLLAQELDLNGVHFYHGRAEDFAQDKNFRAQYDFVTARAVARMQVLSELT
                     IPYLKVGGKLLALKASNAPEELLEAKNALNLLFSKVEDNLSYALPNRDPRYITVVEKK
                     KETPNKYPRKAGMPNKRPL"
     gene            12459..13742
                     /locus_tag="VYN69_000806"
     CDS             12459..13742
                     /locus_tag="VYN69_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007554578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEV1533109.1"
                     /translation="MKQESTVDLLLDVDQRPSAGKGILLSFQHVFAMFGATILVPLIL
                     GMPVSVALFASGVGTLIYMIATGFKVPVYLGSSFAFITAMSLAMKEMGGDVSAAQTGV
                     ILTGLVYVLVATSIRFVGTKWIDKLLPPIIIGPMIIVIGLGLAGSAVTNAGLVADGNW
                     KNALVAVVTFLIAAFINTKGKGFLRIIPFLFAIIGGYLFALTLGLVDFTPVLEANWFE
                     IPGFYLPFSIGGAFKEYNLYFGPEAIAILPIAIVTISEHIGDHTVLGQICGRQFLKEP
                     GLHRTLIGDGIATSVSAFLGGPANTTYGENTGVIGMTRIASVSVIRNAAFIAIALSFL
                     GKFTALISTIPNAVLGGMSILLYGVIASNGLKVLIKERVDFAQMRNLIIASAMLVLGL
                     GGAILKLGPVTLSGTALSAMTGIILNLILPYENKD"
     gene            complement(13937..15508)
                     /gene="ffh"
                     /locus_tag="VYN69_000807"
     CDS             complement(13937..15508)
                     /gene="ffh"
                     /locus_tag="VYN69_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEV1533110.1"
                     /translation="MAFESLTERLQNVFKNLRKKGKISESDVQEATKEIRLALLEADV
                     ALPVVKDFIKKVRERAVGHEVIDTLNPAQQIIKIVDEELTAVLGSDTAEIIKSPKIPT
                     IIMMVGLQGAGKTTFAGKLANKLKKEENARPLMIAADIYRPAAIDQLKTLGQQIDVPV
                     FALGTEVPAVEIVRQGLEQAQTNHNDYVLIDTAGRLQIDELLMNELRDVKALAQPNEI
                     LLVVDAMIGQEAANVAREFNAQLEVTGVILTKIDGDTRGGAALSVRHITGKPIKFTGT
                     GEKITDIETFHPDRMSSRILGMGDMLTLIEKASQEYDEQKALEMAEKMRENTFDFNDF
                     IDQLDQVQNMGPMEDLLKMIPGMANNPALQNMKVDERQIARKRAIVSSMTPEERENPD
                     LLNPSRRRRIAAGSGNTFVEVNKFIKDFNQAKQLMQGVMSGDMNKMMKQMGINPNNLP
                     KNMPNMGGMDMSALEGMMGQGGMPDLSALGGAGMPDMSQMFGGGLKGKIGEFAMKQSM
                     KRMANKMKKAKKKRK"
     gene            complement(15520..15852)
                     /locus_tag="VYN69_000808"
     CDS             complement(15520..15852)
                     /locus_tag="VYN69_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009660419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEV1533111.1"
                     /translation="MEIEKTNRMNALFEFYAALLTDKQMNYIELYYADDYSLAEIAEE
                     FGVSRQAVYDNIKRTEKILEDYEMKLHMYSDYIVRSQIFDQILERYPKDNFLQEQIEI
                     LTSIDNRE"
     gene            complement(15943..16320)
                     /locus_tag="VYN69_000809"
     CDS             complement(15943..16320)
                     /locus_tag="VYN69_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEV1533112.1"
                     /translation="MKIRMRNTIQFDEQLEVIDQLYDVEVHEKRDYSYLLFYNEEKEK
                     VVIKFHGQELVMSRFSNPKTIMRFLKDSDSLAYILTPMGMQEFTIQTSHYQVDRQKIE
                     LDYQLQNQEGHPFASYQLEITWG"
     gene            16392..17696
                     /locus_tag="VYN69_000810"
     CDS             16392..17696
                     /locus_tag="VYN69_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001003495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEV1533113.1"
                     /translation="MNEKVFRDPVHNYIHVNNQIIYDLINTKEFQRLRRIKQLGTSSY
                     TFHGGEHSRFSHCLGVYEIARRITEIFEEKYPEEWNPAESLLTMTAALLHDLGHGAYS
                     HTFEHLFDTDHEAITQEIIQNPETEIHQVLLQVAPDFPEKVASVIDHTYPNKQVVQLI
                     SSQIDADRMDYLLRDSYFTGASYGEFDLTRILRVIRPIENGIAFQRNGMHAIEDYVLS
                     RYQMYMQVYFHPATRAMEVLLQNLLKRAKELYPEDKNFFARTSPHLLPFFEKNVTLTD
                     YLALDDGVMNTYFQLWMTSPDKILADLSQRFVNRKVFKSITFSQEDQDQLASMRKLVE
                     DIGFDPDYYTAIHKNFDLPYDIYRPESENPRTQIEILQKNGELAELSSLSPIVQSLAG
                     SRHGDNRFYFPKEMLDQNSIFASITQQFLYLIENDHFTPNKN"
     gene            17709..18515
                     /gene="yidA"
                     /locus_tag="VYN69_000811"
     CDS             17709..18515
                     /gene="yidA"
                     /locus_tag="VYN69_000811"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEV1533114.1"
                     /translation="MSIKLIAVDIDGTLVNSQKEITPEVFSAIQDAKEAGVKVVIATG
                     RPIAGVAKLLDDLQLRDEGDYVVTFNGALVQETATGHEIISESLTYEDYLDMEFLSRK
                     LGVHMHAITKDGIYTANRNIGKYTVHESTLVSMPIFYRTPEEMAGKEIVKCMFIDEPE
                     ILDAAIEKIPAEFYERYSINKSAPFYLELLKKNVDKGSAITHLAEKLGLTKDETMAIG
                     DEENDRAMLEVVGNPVVMENGNPEIKKIAKYITKTNDESGVAHAIRTWVL"
     gene            complement(18570..18641)
                     /locus_tag="VYN69_000812"
     tRNA            complement(18570..18641)
                     /locus_tag="VYN69_000812"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18606..18608),aa:Arg,seq:cct)
     gene            complement(18683..19030)
                     /gene="rplS"
                     /locus_tag="VYN69_000813"
     CDS             complement(18683..19030)
                     /gene="rplS"
                     /locus_tag="VYN69_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005590628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEV1533115.1"
                     /translation="MNPLIQSLTEGQLRTDIPSFRPGDTVRVHAKVVEGNRERIQIFE
                     GVVIARKGAGISENYTVRKISNGVGVERIFPIHTPRVEKIEVVRYGKVRRAKLYYLRA
                     LQGKAARIKEIRR"
     gene            complement(19149..19478)
                     /gene="crcB"
                     /locus_tag="VYN69_000814"
     CDS             complement(19149..19478)
                     /gene="crcB"
                     /locus_tag="VYN69_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1533116.1"
                     /translation="MVIVYLAIACGFGALVRYFFSRYNQASKLPLGTLIANLLGCFLI
                     GVFYNHVESKEVYAILATGFCGGLTTFSTLNDELQRLLSDKKVFYSYLILTYLGGLVA
                     IFLGILL"
     gene            complement(19472..19846)
                     /gene="crcB"
                     /locus_tag="VYN69_000815"
     CDS             complement(19472..19846)
                     /gene="crcB"
                     /locus_tag="VYN69_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000712114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HEV1533117.1"
                     /translation="MKKEQFYPLGIFLAAMLGGLVRYLVSTWLPASPDFPWGTLFVNY
                     LGIFCLIFLVKGYLVYKGTSKGLILALGTGFCGGLTTFSSLMLDTVKLLDTGRYFSLV
                     LYLLLSIGGGLLLAYFLGRKKW"
     gene            complement(19843..20109)
                     /locus_tag="VYN69_000816"
     CDS             complement(19843..20109)
                     /locus_tag="VYN69_000816"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEV1533118.1"
                     /translation="MDLDIIRQEIDQIDDQIVKLLEERMHLVEGVVAYKKASGKPILD
                     TKREEVIFEKVRSRVEDKRYQETIVATFSDILKRSRDYQDQNIK"
     gene            complement(20225..20668)
                     /locus_tag="VYN69_000817"
     CDS             complement(20225..20668)
                     /locus_tag="VYN69_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEV1533119.1"
                     /translation="MALAKIVFASMTGNTEEIADIVADKLRDLGLDVDVDECTTVDAS
                     DFLEADIAIVATYTYGDGELPDEMMDFYEDLADLNLNGKIYGVVGSGDTFYDEFCKAV
                     DDFDRVFVSIGAEKGSECVKVDLSAEEEDIERLEQFAEELVAKVV"
     gene            20772..21707
                     /locus_tag="VYN69_000818"
     CDS             20772..21707
                     /locus_tag="VYN69_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEV1533120.1"
                     /translation="MEICQQILEKIKEYDTIIIHRHMKPDPDALGSQVGLKALLEHHF
                     PEKTIKAVGFDEPTLTWMAEMDLVEDRAYQGALVIVCDTANTARIDDKRYSQGDFLIK
                     IDHHPNDDVYGDLSWVDTSSSSASEMITLFAQTTQLALADRDAELLFAGIVGDTGRFL
                     YPSTTARTLRLAAYLREHNFDFAALTRKMDTMSYKIAKLQGYIYDHLEVDENGAARVI
                     LSQEVLKQYNVTDAETAAIVGAPGRIDRVSLWGIFVEQADGHYRVRLRSKIHPINEIA
                     KEHDGGGHPLASGANSYSLEENEIIYQKLKNLLKN"
     gene            21761..22045
                     /locus_tag="VYN69_000819"
     CDS             21761..22045
                     /locus_tag="VYN69_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEV1533121.1"
                     /translation="MARKETMAERKYCKMKKDIHPEYRPVVFMDTTTGYQFLSGSTKR
                     SNETVEFEGETYPLIRVEISSDSHPFYTGRQKFTQADGRVDRFNKKYGLK"
     gene            complement(22102..22320)
                     /locus_tag="VYN69_000820"
     CDS             complement(22102..22320)
                     /locus_tag="VYN69_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533122.1"
                     /translation="MSISPRFETLEQAIASKDLEKVREAFKKMNSTWTINESVVRYNS
                     IAHYGRVETAISFLPSSMETEPTDESGT"
     gene            complement(22339..22776)
                     /locus_tag="VYN69_000821"
                     /pseudo
     CDS             complement(22339..22776)
                     /locus_tag="VYN69_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT         6699 a         5097 c         4375 g         6605 t
ORIGIN      
        1 actgttctaa attcagtctg ctatatctta tttttctatt taaatcgctt ctgtaacaaa
       61 gctacgactt tcaagtacct taagcatggc attagctgta tctagcgctg tgaagagggg
      121 caccccgtgt tcaatggctg aacgacgaat ttgctcacca tcttcgtcag cagttcgttt
      181 tgttcctact gtgttaatga tagcttgaat tcttcctttg cgtacaaaac ttgggatatc
      241 cttatcgtca tcaccaatct taccaacagg ttgggcttgc aagccatgac tagcaaagaa
      301 ggctgctgtc ccttctgtcg caaggattcc ataaccaatg ttttggaaac gacgagccaa
      361 gttcaaggct tcttctttgg catcatcagc gatggtaaag acgacattac caaaagttgg
      421 caagtgtaga taagaagctt caaaggcttt atagagagct ttttccaaag tagcatcaga
      481 acccataact tcacctgttg acttcatttc aggaccgagc aagctgtcta ccttagctag
      541 tttggtaaag gagaagacag gtgccttgat atgaacacgg gtgctttcag ggtaaagtcc
      601 attttggtag ccaagttctg ataaactttg accaagaatg agcttggtcg ctacttgagc
      661 cataggaata ttggttacct tagataggaa tggaacggta cggctggcac gtggattgac
      721 ctcaataacg tagacttttt catccttgat aacaaactgg atgttcatca ttccaaggca
      781 gtgaagaccg attgctaagc gtttggtgta gtctgcgatg gtctcctgaa ccttttgcga
      841 caaggtttgt ggtgggtaaa cagccattga gtcacctgag tggacaccag cacgttcgat
      901 atgctccatg ataccaggaa tgagtacatt tttaccatct gaaatggcat caacttcgca
      961 ctcttgccca acgatataag agtcgacaag aactggatgg tctggactag ccttaacagc
     1021 agttcgcatg taagaacgaa ggtcttcttc gttttcaacg atttccatgg cacgtccacc
     1081 aagtacataa gatgggcgga caagaactgg gaagccaatc ttacgagctg caagagctgc
     1141 ttcttcttca ttggtagccg tttgtcctgg tggctgtgga atatccaatt ctttgagagc
     1201 ttgctcgaag aggtcacggt cttcggcacg gtctaggtca gcaacctgtg taccaaggat
     1261 ggtcacacct gcttttgcca atggctccgc aaggttgatg gctgtttgac caccgaactg
     1321 aacgataact ccctttggtt gttccaagtc aatgacgttc ataacatctt cgaatgtcaa
     1381 tggctcaaag taaagcttat ctgatacaga gaagtctgtt gaaacggtct ctgggtttga
     1441 gttcatgatg atagcttcat aaccagctgc ctggatagcc ttaacagagt gaacggttgc
     1501 gtagtcaaac tcaacccctt gaccgatacg gattggacct gaacctagga caagtacaga
     1561 ttctttatca gatctgatag attcattttc ccaaccatag gttgaataga aatatggcgt
     1621 ttcggagtcg aactctgccg cacaagtgtc taccatctta taaactggaa caatcttgtt
     1681 ttccaagcga agttggcgaa ctttatcatc agtcgttccc cagagttcag caatcttacg
     1741 gtctgaaaaa ccattaagtt tggctgtttt caaaacttct agatcttgtg gatgagcacc
     1801 caattcttgc tcaatttcaa agatatgcaa gagtttatca agatagaaga tatcaatttt
     1861 tgtaagctct gcaatttctt caggtgtgta gccacgacga atggcttctg atacgtagaa
     1921 gagacggtca tcttgggctt tgacaacctt ttcaatcaag gcatcatcag aaactgctgc
     1981 aagttcaggt atttcattgt ggtgcacccc aatttcaagg gagcggcagg ccttgagaag
     2041 agattcctcg atgttacgac cgattgccat gacttctcca gtcgccttca tttgtgtacc
     2101 gagacggcgt tcaccctttt caaacttgtc aaatgggaaa cgtggaatct tagcaactac
     2161 gtagtcaagg gctggttcaa acatggcata ggttgaacct gtaactgggt ttataacctc
     2221 atccaaggtc aaacctactg caatcttggc agccaactta gcaatcggat atcctgtcgc
     2281 tttagaagca agggctgacg aacgtgatac acgagggttt acttcgataa cataatactt
     2341 gaagctgtta ggatcaagag ctagctgaac attacatcca ccttcaatct tgagggcacg
     2401 aataatgctc aagctcgcat cacgaagcat ttggttttca tagtctgaca tggtttgcgc
     2461 aggggcaaat acaatggaat cccctgtgtg aatcccaact gggtcaaagt tttccatgtt
     2521 acaaacaacc aaggcattgt cagctgagtc acgcatcact tcgtattcaa tttccttgaa
     2581 accggcaatc gaacgctcaa tcaaacattg ggtaacaggt gacaatttca aaccattttc
     2641 agtgatttca cgcaattctt tctcgttggc acacatacca ccaccagtac caccaagggt
     2701 aaaggctgga cgaacgatga ctgggtagcc aattgtcgct gcaaaggcaa ctgcttcttc
     2761 tactgtgtta acaatttcag attctggaat gggttgttca agctcttcca tcaattgttt
     2821 aaagaggtca cggtcctccg cttggtcaat ggcagataat ttggtaccca gaagttcaac
     2881 gccaagctcg tctaggatac catttttaga taattccatg gccatgttga gacctgtctg
     2941 accaccgagt gttggtagca aggcatctgg accttcctta cgaagaatac gtgtcacaaa
     3001 ctcaagtgta atcggttcaa tgtaaacctt gtcagcaatt tccttgtccg tcatgatggt
     3061 tgcaggattt gagttaacca aaacaacctc ataaccttcc tctttcaacg acaagcaagc
     3121 ctgagtccca gcgtagtcaa actcagcagc ctgaccaata ataatcggac cagaaccaat
     3181 caccataatt ttttgaatat cagtacgttt aggcatatat aagatattaa gggcgtcaag
     3241 cggacaaagc taaaatagga gttatgacga agaactgtca gttctaggaa taactatctt
     3301 tttagcaccg tccgtagccc gtattcagtt cagcaaatac ggagcaccct tctcctttct
     3361 attcgtcgcc tctcagggcg acattaaata agatacaaag gacgaataga aagcgattga
     3421 attttaggaa atcaaggaag gattgacaat ccaagttggt ttctctaaat tctgagcttt
     3481 ccgtccatgt tcagttacat aaattctccg acgagctttt actcattctt agtttgattg
     3541 tttaaaaact tccatcatct cgataaactc gtcaaatagg tagctagcgt cgtgtggccc
     3601 aggagctgca tctgggtggt attgaacaga gaaagcaggt tggtatctgt ggcgcacacc
     3661 ttccactgac ttgtcattga tttcttcgtg ggtaataatc aagtgctctg gcaaatcctc
     3721 gcggctgact gcataaccat ggttctggct ggtgaagtct actcgtcctg ttgcgatttc
     3781 acgtaccgca tggttgaatc cacggtggcc aaacttcatc ttataggtct tagccccgtt
     3841 tgccattgca aagagttggt gtcccataca aataccaaag attggaattt ttccttgtac
     3901 accgcgaatc atgtcgagtg cttgtggaac gtcttctggg ttacctggac catttgacaa
     3961 cataactcca tcaggattga gatggagaat ttcttcagcc tttgtcgaat aaggaacaac
     4021 tgtcacgtta cagttgcgtt tagaaagttc acgtaggatt gagtgcttga gaccaaagtc
     4081 cactagcacc acgctcaaac caactcctgg agctggataa gacgttttag tagaaacctg
     4141 tttgatattg tctgtcggta aaactgttgc ttggagctgg tccgtcacat ggtccatact
     4201 gtccccaaca tgggtcaagg ttgcacgcat agtaccatgc ttacggataa tcttggtaag
     4261 agcacgcgta tcaattcctg aaatccctgg aattttcttg gctttcaaaa attcatccaa
     4321 ggtcatttgg ttgcgccagt tgctagctct acgcgcttct tcaaaaacaa cgactccctt
     4381 acaagttgga ataatggatt cataatcatc acgattaata ccataatttc ctaccaaagg
     4441 ataagtaaag gtcaagattt gtccattata agactggtct gtaatggatt cttggtagcc
     4501 ggtcatccct gtattaaaga cgatttcgcc tgttacatca atatctgctc cgaaggcctt
     4561 gccttcaaaa actgtgccat cttctaatac tagaattctt tttgtcatat tttcacctct
     4621 cgtggacgct cactggcgtc ttttaacgtc ttgtgtttta gttggcgttt ctactcgcta
     4681 gtacggattc taagattgcc attcgaacaa agacaccatt ggtcatttgt tggacaatcc
     4741 gtgattttgg tgcttcaacc aagtggtctg ctatttctac atcacgattg attggagctg
     4801 ggtgcatgag gattgctgtt tctttcaaac gatcgtaacg ttcttgagtc aagccatgtt
     4861 gggcatggta gtcttctttt gaaaatacag ctccactatc atggcgttcg tgttgcacac
     4921 ggagaaacat catgacatca acctgatcaa tgatttcatc aatggttaca aactgtccat
     4981 agtctgcaaa ctcttgactt ctccattcct caggtccagc gaaaaagagt tcagatccca
     5041 agcgtttcaa aatctgcata ttggatttgg caacgcgtga gtggtccaag tcacctgcaa
     5101 tagcaacttt aagaccctca aagtggccaa attcctcata aatggtcatc aaatcaagca
     5161 agctctggct agggtgttgg cccgaaccat ctccaccatt gatgatggaa gtcgtaatcg
     5221 ttggactagc aatcaattct ctatagtagt cgacctctgg atggcgaatc acacagacat
     5281 ccactcctaa agcagacaga gtcaaaatgg tgtcataaag tgtctcaccc ttattaaccg
     5341 aactagtctt cacatcaaag tcaagtcgtt ccaatccaag tttaatctct gcgacttcaa
     5401 aggacttatg tgtccgtgta gaatcctcaa agaagagatt ggaaacaatc ggatggtctt
     5461 catagggaag ctgggctcca tttttaaact caattcctcg cttgatcaat ttcattactt
     5521 gatcgacagt gaggtcttcc atggacacca catggttcaa tgcttgttga ttttctgaca
     5581 tggctactcc tttaactttc taagcttctt cagtaatcag aactctgtct tggtcatcaa
     5641 gttctgtcat ctctacgatg atttcttcag aacgactggt tgggatattt tttccaacgt
     5701 aatctggacg gattggcaat tctctatgtc cacgatcgac tagaactgct aaactcacac
     5761 gcgcaggacg accatgaccg acaatattat caatagcagc acggatggta cgacctgtat
     5821 agagcacatc atccaccaag ataacttcgc ggtctgtcac atcgacagaa accaaagaag
     5881 tatcttctcc acttttaaca tcatcacgga aaggtttagt atccaattcc acaacaggaa
     5941 ctgaaagatt ttctaactgc ttcaaacgtt cttggattcg gtgggcaata aagacaccac
     6001 gagttttaat accagccaag acgatcttat tcaaatcttt gttgcgttcg ataatctcat
     6061 aagtaatacg cgtaatcgct cgtttgacgg tcaattcgtc tacaacttct tttgttttca
     6121 tgacaaacct ccaaaaagaa aagtctcctt aaacaaggag acttgaaatt tatagccaag
     6181 cgagccctac tgcacacagt atagacttca cccttctact ttatcgcgct ccttgcctgc
     6241 ctcacgggac aggtttaaag gaatatttag ttatcattta ctatagcaca aagcatgctt
     6301 aaaatcaagc aaaaagtttc aatgtagcat cttacaaatt gctaaaatca tataattgtg
     6361 ggtactggtc acactctgga ttttttggat ggcaaatggc tcttccaaaa taaatcatgg
     6421 cctgatgggc agctaaccac tgctcaggcg gcaagatatc catgacccgc ttttccacct
     6481 caagtggcgt cgctgatttt ttgacaatat cgtggtgttt gcaaatacgc tccacatgag
     6541 tatccactgc aaaggctgga atcccaaatc ctacactcat gacaacattg gctgtcttgc
     6601 gaccaacacc tgccaaactc tccaattctt cacgtgtctg agggacttga ccatcaaaat
     6661 cgtctagtaa ctgttgggca cattttttaa ggaatttagc tttattccga tacaatccca
     6721 agcgagaaat atgtgaagca atctcactct ctgtcgctac agacatagct tggggtgttg
     6781 gaaaggcaac aaagagacct ggtgtggcct tatttaccgc tgcatctgtc gtctgggctg
     6841 ataacatgac cgcaaccagg agttcaaaat gattggtaaa atcaagacta ggcttggcat
     6901 ctgggaagag ggcaatgatt tcttctagca cctttcgtgc tcgttttttt gacaagacca
     6961 ttattcatct ccgtcaaata gtccttgtaa gccagcaaaa ggactgtttt cttctttctt
     7021 tactgcttgt tgagcttggt attcttcctc tgtcatgatt tgccagtcat ttcctgagac
     7081 aaatccttga ccagcttctt cttcagccgt caagaccttg ataggaatgt ttagcaagat
     7141 attgtctgat acactctcag caaggtcaag cgcctcattt tcgatgggca agaccaagtc
     7201 atcatctaaa atttcttgat ttagatggtt agttgcgcct tccatgaaaa cttccgtgac
     7261 tggataagat tcaactaact caactggctc catactgcga ctcgaagcaa gaacaatggt
     7321 ataagatagt tgataatcta agaaatacat acggtcttca tattgtactt tcccaactgc
     7381 aaggatatct tttacatcta aaatttcttg attacgtgca cgcaggtcat caactaaatc
     7441 taacgtttgt tcaaagttca aaccttcaga ctgcttacga atttcttgaa tatttaattt
     7501 catacttcct ccataaagat ttactctctt gattatacca tgaaaaggct acaaatcagc
     7561 acaccaaact ttgtaattaa aattcaaaat tttaacatat ttactatgac agttttgttt
     7621 tttagtgcta tactatagag aaagagtaca tcagatcaag gaggatgctc acatggaaga
     7681 caagaaactc attcaactcc tatccaagtt aaataaaagc taccaaaact gtaaacaggg
     7741 tacggcagat gatattcgac tacaagagct gctaaacact actatgcaag agctcaaaaa
     7801 aacggaaaag ttgaacaaca gtatcttaat tgatcttgag aaattttacc aacccaccag
     7861 tcttctgatt ggactgggta gcctaaaact aaacgatcaa gcacgcactg cttggcgaaa
     7921 ctatgataaa ttccattacg agcatgtcaa acacgtacta agtctctatg gacctgtttt
     7981 tggattttag agcatagaat ttccagtttt ctgttgacaa aatttcctta aaggtataat
     8041 ataaagatac taatactcgg aggtaaggga gacatgaaca actaagtcta tcaaataaag
     8101 aacctttatt tagtagatct tgtttttgtc tctttttgtg tgctctttta tgctcttttt
     8161 ctggcatgtt aatagagttt ttttgacata gactttgggc tctactaggt aaagtagagc
     8221 tttttgttat gcactatcga cattctagaa agggcaatag tatgataaaa atcaatcacc
     8281 tgaccatcac acaaaacaaa gatttacgag accttgtctc tgacctaagc atgaccatac
     8341 aagacgggga aaaggtagct attattggcg aagaaggaaa tggtaaatca accttactta
     8401 aaactttaat gggggaagat ttgtctgatt tcactatcaa gggagacatc cagtctgatt
     8461 atcagtcact ggcctacatt cctcaaaaag tccctgagga cctaaaaaag aaaactttac
     8521 acgactactt ctttttagat tctattgatt tagactacag tatcctctat cgtttggcgg
     8581 aggaattgca ttttgatagc aatcgtttcg caagtgacca agaaattggc aatctatcag
     8641 ggggcgaagc tttgaaaatt cagcttatcc atgagttagc caaacccttt gagattctat
     8701 ttttagatga accttcaaat gacctagacc ttgagacagt tgattggcta aaaggccaga
     8761 ttcaaaagac caggcaaacc gttattttca tttcccatga tgaagacttt ctttctgaaa
     8821 cggcagacac tattgttcac ttgcgactgg tcaaacaccg taaagaagcg gaaacgctag
     8881 tagagcattt agactatgat agctatagtg agcagagaaa ggctaatttt gccaaacaaa
     8941 gtcagcaagc tgctaacaac caaagagcct acgataaaac catggaaaaa catcggagag
     9001 tcaagcaaag tgtagaaact gcgcttcgag ctaccaaaga tagtactgcc ggtcgcctat
     9061 tggctaaaaa gatgaaaact gtcctctcac aagaaaaacg ctacgaaaag gcagctcagt
     9121 ccatgactca aaagccactt gaagaggaac aaatccaact tttcttttca gacatccaac
     9181 cattaccagc ttctaaagtc ttagtccaac tggaaaaaga aaatttgtcc attgacgacc
     9241 gagttttggt tcaaaaacta caactaactg tccgtggcca agaaaaaatc ggtattatcg
     9301 ggccaaatgg tgttgggaaa tcaactctgt tagccaagtt acagagactt ctgaatgata
     9361 aaagagagat ttcacttggt tttatgccac aagattacca caaaaaactg caattggatt
     9421 tatccccaat agcctatctc agtaaaactg gggaaaaaga ggaactacag aaaatccaat
     9481 ctcacctagc tagtctcaat ttcagttatc cagaaatgca gcatcaaatt cgctccttat
     9541 ctggcggaca acagggaaaa ctcctgcttt tggatttagt cctgcgcaaa ccaaactttc
     9601 tcctgctgga tgaacccaca cgaaactttt ctcccacttc tcaaccccaa atcagaaaac
     9661 tctttgccac ttatcctggc ggtctcatca ctgtttcgca tgaccgtcgt ttcttaaaag
     9721 aagtctgctc gatcatctat cgcatgacag aacacggttt gaagctagtt aatttagaag
     9781 atttataaat ttgcaacata gcagataggt ggggcaaaaa cacaatttta ggagaaaaag
     9841 aagtaaatct tcccatagta aaacgcataa tatcaagttt tcacacctga tattatgcgt
     9901 ttttcagatt ttaaagactt ttttccagcc ttcattttac atctgtttta aacgttcaat
     9961 ccgttctgag ataggtgggt gggtataaaa gagtttttgg aaccccccac ctttcttggg
    10021 atcattgata taaagggcac tgctagcatc atcgacgtgg cgactcatag gtttgctatt
    10081 gtccaactta tctagggcat taatcattcc ctggggattg cgagtcagct cgacactaga
    10141 tgcatctgcc agaaattccc tctgacgaga aatagcgagc tgaaccaagg ttgcagcgag
    10201 aggtgccagt acaatagcta gtagggaaac cactagcaaa atgatttcaa gaccatttcc
    10261 atctcggtca tcatcacttc gtctgcgacc tgctccaccc caccacatca tacgacctgc
    10321 catactagaa agcatggtga tagcactagc aagggcaact gcagtagtcg aaatacggat
    10381 atcataatta cgaatatgac tgacttcatg tcccataaca gcttctagtt cttcacgatt
    10441 catgatagct agtagacctg aagtcgcagc aacagccgca ttttgaggat tagaacctgt
    10501 cgcaaaggca tttaaggctg gatcatcaat gatgaaaaca cggggcatag gaatctgagc
    10561 gaccagagcc atatcttcca ctacatggta gagatctggt gccgtttgct catccacctc
    10621 acgcgctcca ttcatggaca tgacaatctc tgtcgattga aaaatcatag acaaagcgta
    10681 gataaagccg ataatcagtg cgataaccaa accaccaagt ccagatctta taaagagata
    10741 accaaccgca taaccaacaa gagctaagag taggaaaaat accagcaaca aaatccaggt
    10801 ttttcgttta ttgcttgcaa tttgatcaaa caacatctta gtcacctaaa ccgctaaaat
    10861 caactttagg aaccgacttt tcctcttcag gtgtttgaag gaaatctgcc gctttaaatc
    10921 caaacattcc agcgataata ttgctcggga aagtttctaa ttttacattg tagttgctga
    10981 caacactgtt atagagttga cgagagtaag aaattttatt ttctgtgttt gtcaactcct
    11041 cttgcaattt aacaaagtta gcactagctt tcaaatctgg atagctttct gcaactgcaa
    11101 aaatacctga aacctgacga gtgagggcat cactggcttt catagcttct gctggtgaag
    11161 tcgctgccgc cacttggtta cgtagttctg ccaccttttc aagggtagaa ccttcatatt
    11221 tggcataacc ttttacagtc tcaatcaagt ttggcaagag atcattgcga cgtttcaact
    11281 gaacatcaat ctgactccaa gcctccttgg tttgcatacg atttttaacc aaaccgttat
    11341 agctaacaat tacaaaaata acaacaagag ctaaaactcc aagaataatc caagtcataa
    11401 tataagtcct ttctgctttt agattagtac cagtatatca aattttctat gattgtggta
    11461 aaataagatg atactaaaga aggaaataac tatgaaacca aaaacatttt acaacttgct
    11521 tgccgagcag aatcttccac tttcggacca gcaaaaagaa caatttgaac gttattttga
    11581 gctcttggtc gagtggaatg agaagattaa tttgacggcg attacggaca aggaagaagt
    11641 ttatctcaaa catttttacg attcgattgc acccattctt caaggtttga ttcccaatga
    11701 aactatcaaa cttcttgata tcggggctgg ggcaggattt cctagtctac caatgaaaat
    11761 tctctatccg gagttagatg tgaccattat tgattcactc aataagcgca tcaacttcct
    11821 acaactcttg gctcaagaac tggatttgaa cggagttcat ttctaccacg gacgtgccga
    11881 agattttgcc caagacaaga acttccgtgc tcaatatgat tttgtaacag ctcgtgcggt
    11941 tgcccgtatg caggtcctat ctgaattgac tattccctac cttaaggttg gtggcaaact
    12001 attagcactc aaggctagca atgcgcctga ggaattatta gaagctaaga atgccctcaa
    12061 tctccttttt agtaaggtcg aagacaatct cagctacgcc ctaccgaata gagatccgcg
    12121 ctatatcaca gtggtagaaa agaaaaaaga aacaccaaat aaatatccac gtaaggctgg
    12181 tatgccaaat aaacgcccac tttaaatttt ttagtaaaca aatgtttaca aaatcagcct
    12241 cgctctttta tttctaggct cgggaaaaaa tgatttacaa aatcattttt ttctgctata
    12301 ctatcctaag caaaggtttt taatgtcatc ccgtgaggtg acgaagacgc agaaatattt
    12361 aaaactcttt aaaatctaaa ttttaaagaa gtcttactct gagggcctat tgctgtaaaa
    12421 taatgggctc ttttttgatg cccaaaagtg aggtttatat gaaacaagaa tcaactgttg
    12481 atttgttact agacgttgac caacgtcctt cggctggaaa aggaattctc cttagtttcc
    12541 aacacgtttt cgccatgttt ggtgcgacca tcttggtacc attgattttg ggaatgcctg
    12601 tatctgttgc cctttttgct tcaggtgttg gaacactcat ctacatgatt gctactggtt
    12661 ttaaagttcc agtttatcta ggttcttcat tcgcctttat cacagctatg tcactggcta
    12721 tgaaagaaat ggggggggat gtatctgctg ctcaaacagg ggttatcttg actggtttgg
    12781 tctatgtcct tgttgctacc agcatccgat ttgtaggaac aaaatggatt gataaactct
    12841 tgccaccaat cattatcggt cctatgatca tcgttatcgg tcttggactt gcaggttcag
    12901 ctgttaccaa tgcaggtctt gtagcagacg gaaattggaa aaatgctctg gtagccgttg
    12961 ttactttcct aattgctgcc tttatcaata caaaaggaaa aggcttccta cgaatcattc
    13021 cattcctctt tgccattatc ggtggttacc tcttcgcact aactcttggt ttggttgact
    13081 ttacgcctgt cctagaagct aactggtttg aaattcctgg tttctacttg ccatttagca
    13141 taggtggtgc ctttaaagag tacaatcttt actttggtcc agaagccatc gctatcttgc
    13201 caatcgctat cgtaacaatt tctgaacata tcggagacca tactgttttg ggtcaaatct
    13261 gtggccgtca attcttaaaa gaaccaggtc ttcaccgtac tcttattggt gacggtatcg
    13321 caacttctgt ttctgccttc cttggtggac cagccaatac aacttacgga gaaaatacag
    13381 gggttatcgg tatgactcgt atcgcttctg tctcagttat ccgtaacgct gccttcatcg
    13441 cgattgccct cagcttcctt ggtaaattca ctgccttgat ttcaactatt ccaaacgctg
    13501 tacttggtgg tatgtcaatc cttctctatg gggttatcgc cagcaatggt ttgaaagtct
    13561 tgattaaaga acgtgttgat ttcgctcaaa tgcgaaacct catcatcgca agtgctatgt
    13621 tggttcttgg acttggagga gctatcctta aacttggtcc agttacactt tcaggtactg
    13681 ccctttcagc catgacagga atcatcttga acttgatctt gccatacgaa aataaagact
    13741 aagagtctaa atacacctaa tccactcaga cagctgagtg gatttttcgt ataccataat
    13801 aaaagtgtct taacaaaatt attaaaatca aaaaacgtat aatatcagat attctaaaac
    13861 cttgatactg tacgttttat catagaaatt tttactttat tttctcatca aatgagattt
    13921 gcatcaatct cttgtcttac ttgcgtttct tcttcgcttt cttcattttg ttagccatac
    13981 gtttcatgga ctgtttcatg gcaaattcac caattttacc tttcaaaccg ccaccaaaca
    14041 tctggctcat atctggcatt cctgctcctc cgagagctga taagtcaggc ataccgcctt
    14101 gtcccatcat tccttcaagg gcagacatat ccattcctcc catatttggc atatttttag
    14161 gaaggttatt tggattaatc cccatttgct tcatcatttt attcatatcc ccagacataa
    14221 caccctgcat gagctgttta gcctggttaa agtccttgat gaatttattg acttcgacga
    14281 atgtatttcc agaaccagca gcaatacgac ggcgacggct tggatttaac aaatctgggt
    14341 tttcacgctc ttcaggtgtc atcgaagaca caatggcacg tttacgagca atctggcgtt
    14401 catccacctt catgttttga agggctggat tgttggccat acctggaatc atcttgagca
    14461 agtcttccat cggccccata ttttgcacct gatctaattg atcgatgaaa tcattaaaat
    14521 caaaggtgtt ttcgcgcatc ttctcagcca tttcaagggc tttttgttca tcgtattcct
    14581 gagaagcttt ctcaatcaaa gtgagcatat cccccatacc aaggatacgg ctagacatgc
    14641 ggtctgggtg gaaggtttca atgtccgtaa tcttttcacc tgtaccagtg aacttgattg
    14701 gttttccagt aatgtgacga acagacagag cagcaccacc acgagtatcg ccatcaatct
    14761 tggtaaggat gaccccagtc acttccaact gagcattaaa ctcacgcgca acattggctg
    14821 cttcctgacc aatcatagca tcaacgacaa gcaagatttc atttggttga gccaatgctt
    14881 tcacatcacg aagctcattc atgaggagct catcaatctg caaacgaccc gcagtatcaa
    14941 tcaagacata gtcgttatga ttagtttggg cttgctccaa accttgacgt acaatctcaa
    15001 cagctggtac ttctgttcca agtgcaaaga caggcacatc aatctgttgt cccaaggtct
    15061 taagctgatc aatagcagct ggacgataaa tatccgccgc aatcatcaaa ggacgagcat
    15121 tttcttcttt cttgagtttg ttggccaatt taccagcaaa ggttgtttta ccagcccctt
    15181 gtaaaccaac catcatgatg atggttggaa tcttaggtga cttgataatt tctgccgtat
    15241 cagaacctaa aacggctgtc agttcctcat caacgatttt aataatctgt tgcgcaggat
    15301 taagtgtatc aatgacctca tgcccgactg cacgctcacg aactttcttg ataaagtcct
    15361 ttacaacagg caaggcaacg tcggcctcga gcaaggccaa gcgaatttct ttggttgcct
    15421 cttggacatc agattcagag atttttcctt ttttacgtag atttttaaag acgttctgca
    15481 aacgttctgt taaactttca aatgccattt ttcttcctct tattctctat tatcaatgct
    15541 tgttaaaatt tctatctgct cctgcagaaa gttatccttg ggatagcgct ccaaaatctg
    15601 atcaaaaatc tgactgcgga caatatagtc cgagtacatg tgcaatttca tctcataatc
    15661 ttccagaatc ttttctgttc gcttgatatt gtcatagaca gcctgacgac tgacaccgaa
    15721 ctcctcggca atttcagcaa ggctgtaatc atcagcgtag tagagctcga tataattcat
    15781 ttgcttatct gtcaaaagcg ccgcataaaa ttcaaagagc gcattcatac gattggtttt
    15841 ttcgatttcc ataactttta ttataccaaa aattagccta atctaccaca ctaggaagcc
    15901 gatccaagaa gatagatagc taaatttgaa aaagacatga gtctagcccc aagtaatttc
    15961 caattgatag ctggcaaagg gatgtccctc ttgattttgt agttgataat ctagttcaat
    16021 cttttgccta tcaacttgat aatggctcgt ttggatggta aactcctgca tgcccatagg
    16081 tgtaagaata taggctaaac tatcgctatc ctttagaaag cgcataatgg tcttgggatt
    16141 agaaaatcgg ctcatcacaa gttcttgacc atgaaattta atcactactt tttccttttc
    16201 ctcattatag aaaagcaggt agctataatc tcttttttca tgcacttcca catcataaag
    16261 ctggtcaatc acttccaact gctcatcaaa ctgaattgta tttcgcatcc gaatcttcac
    16321 atcaggtcct ctttcttgtc tcttgtccta ctattttacc aaaaagagca ggattttgct
    16381 ataatggtca tatgaacgaa aaagtattcc gtgacccagt tcacaactac atccatgtca
    16441 ataatcaaat catctatgac ttgattaata caaaagaatt tcaacgtttg cgccggatca
    16501 agcaactagg gacttccagt tataccttcc acggtggaga gcatagtcgc ttctctcact
    16561 gtctgggagt ctatgagatt gcacgacgta tcacagaaat ttttgaagaa aaatatcctg
    16621 aggaatggaa tcctgccgag tctctcttga ccatgaccgc tgctctccta catgaccttg
    16681 ggcatggcgc ctactcccat acttttgaac atctctttga tacagaccat gaagccatta
    16741 ctcaggagat tattcaaaat cctgagacag agattcacca agtcttgcta caagtagcgc
    16801 ccgatttccc agaaaaggtg gccagtgtca ttgaccatac ctatcctaat aagcaggtcg
    16861 tgcagctcat ttctagtcag attgacgcag atcgcatgga ctatctcttg cgcgactcct
    16921 attttacagg agcatcctat ggggaatttg acctgactcg catcctccga gtcattcgtc
    16981 ctatcgaaaa tggtatcgcc tttcagcgca atggcatgca cgccatcgaa gactacgtcc
    17041 tcagtcgcta ccagatgtac atgcaggttt atttccaccc cgcaacacgc gccatggaag
    17101 ttctcctgca gaatctcctc aaacgcgcca aggaactcta tcctgaggac aagaatttct
    17161 ttgcacgaac ttccccacac ctcctgcctt tctttgaaaa aaatgtgacc ttgactgact
    17221 atctggctct agatgatggt gtgatgaata cctactttca actctggatg accagtcctg
    17281 acaagattct cgcagacttg tcacaacgct ttgtcaaccg caaggtcttt aaatccatta
    17341 ccttttcaca agaagatcaa gaccaactcg ctagcatgag aaaattggtt gaggacattg
    17401 gctttgatcc cgactactat actgccattc ataagaactt tgacctccct tatgatatct
    17461 atcgtcccga atctgaaaat ccacggacac agattgagat tttacaaaag aatggagaac
    17521 tggccgaact ctctagcctg tctcctatcg tccaatccct tgctggtagt cgtcacggag
    17581 ataatcgttt ctattttccg aaagaaatgt tagatcaaaa cagtatcttt gctagcatta
    17641 cccagcaatt tttatacttg attgagaacg atcattttac cccaaataaa aactagaaga
    17701 ggaaatttat gagtattaaa ctaattgccg ttgatatcga cggaaccctt gtcaacagcc
    17761 aaaaggaaat cactcctgaa gttttttctg ccatccaaga tgccaaagaa gctggtgtca
    17821 aagtcgtgat tgcaactggc cgccctatcg caggcgttgc caaacttcta gacgacttgc
    17881 agttgagaga cgagggggac tatgtggtaa ccttcaacgg tgcccttgtc caagaaactg
    17941 ctacaggaca tgagattatc agcgaatcct tgacttatga ggattatcta gatatggaat
    18001 tcctcagtcg caagctcggt gtccacatgc atgccattac caaggacggt atctatactg
    18061 caaatcgcaa tatcggaaaa tacactgtac acgaatcaac cctcgtcagc atgcctatct
    18121 tctaccgtac ccctgaagaa atggctggca aagaaattgt taaatgtatg tttatcgatg
    18181 aaccagaaat tctcgatgct gcgattgaaa aaattccagc agaattttac gagcgctact
    18241 ccatcaacaa atctgctcct ttctacctcg aactccttaa aaagaatgta gacaagggtt
    18301 cagccattac tcacttggct gaaaaactcg gattgaccaa agatgaaacc atggcaatcg
    18361 gtgacgaaga aaatgaccgt gccatgctgg aagtcgttgg aaaccccgtt gtcatggaaa
    18421 atggaaatcc agaaatcaaa aaaatcgcca aatacatcac taaaacaaat gacgaatccg
    18481 gcgttgccca cgccatccga acatgggtac tgtaaaagga aagaatagac taaaaccgct
    18541 cagctgagcg gtttttgatg tctaattact gccccctgca ggaatcgaac ctgcaactac
    18601 tccttaggag ggagttgtta tatccattga actaagggag ctagataaaa actctgctga
    18661 aaagcagagt tttttagtcg aattaacgac gaatttcttt gatacgagct gctttacctt
    18721 gaagagcacg caagtagtac aatttcgcac gacgtacttt accgtaacga acaacttcga
    18781 ttttttcaac acgtggagtg tggattggga agatacgctc aacacctaca ccgttagaga
    18841 ttttacgaac tgtgtagttt tctgagattc cagcaccttt acgtgcgata acaacacctt
    18901 caaaaatctg gatacgttca cggttacctt cgacaacttt cgcgtgtaca cgaacagtgt
    18961 caccaggacg gaatgatggg atatctgtac gaagttgacc ttcagtcaag ctttggatta
    19021 atggattcat tttattctcc tatctttgtc aatcttgagg aatcttcctc agcggataaa
    19081 ctgtattttt gtgcgtccat tacacacaag atatagttta ccaaatttct aaaaaaaagt
    19141 aaagaaattt atagcagaat tcctaaaaaa atcgcaacca aaccacctag gtaggtcaaa
    19201 atcaggtaag aataaaatac cttcttatcg cttagcaacc tttgaagttc gtcattcaag
    19261 gtcgaaaaag ttgttaaacc gccacaaaat cctgttgcta gaatagcata tacttcctta
    19321 gactctacat gattgtagaa tactccaatt aaaaaacaac ctaaaagatt agctatgagc
    19381 gttccgagtg gcaatttaga agcttgatta tagcgagaaa agaaataccg tacgagtgct
    19441 ccgaaaccac aagcaattgc aagatagacg attaccattt cttcctccct agaaaataag
    19501 ctaagagtag gcctccaccg atgctcaaaa gcaaatacag gactaaacta aaataacgcc
    19561 ctgtatcaag cagcttcaca gtatcaagca ttaggctgga aaaagttgtt aaacctccgc
    19621 aaaaacccgt ccccagtgct aaaatcaagc ccttactagt ccccttatag accagatagc
    19681 ccttgacaag aaaaatcaag cagaaaattc ccagatagtt gacaaagaga gtgccccaag
    19741 gaaagtctgg actggctggt aaccaggtgg aaactagata tcggacaagt ccgcccaaca
    19801 tagcagctag aaaaattcct agcggataaa attgttcttt tttcatttga tattttgatc
    19861 ctgataatca cgcgaacgtt tgagtatgtc cgaaaaagtc gctacaatag tctcctgata
    19921 gcgcttgtcc tctacacgac tcctaacctt ttcaaaaata acttcttctc tcttagtatc
    19981 taaaatcggt ttacctgaag ctttcttata agcaaccacc ccctcaacta aatgcattcg
    20041 ttcttctaac agtttgacaa tttggtcgtc gatttgatcg atttcttgac gaataatatc
    20101 taaatccata cagtctcctc ctttatttga attattgtat caaaaagcca tcaattaggc
    20161 tagtaaaatc ccaactctcg ataagaaaaa tgcactgatt gattacatca gcgcactttt
    20221 atgcttatac tactttagca accaattctt ctgcgaattg ttccaagcgt tcaatatctt
    20281 cttcctcggc agaaagatca actttaacac actctgaacc tttttctgct cctattgaca
    20341 caaagacacg gtcaaagtca tcaacagcct tacagaattc atcgtagaag gtatctcctg
    20401 agccgaccac tccgtagatt ttgccattca agttgagatc tgctaggtct tcgtagaagt
    20461 ccatcatctc atctggcaat tcaccatcac cataagtata ggtcgcaacg atagcgatgt
    20521 ctgcttccaa gaagtctgaa gcgtcaacag ttgtacattc atcaacatca acatccaagc
    20581 ccaagtcacg taatttgtct gctacaatat ctgcaatttc ttcggtatta ccggtcatac
    20641 tggcaaatac aatttttgct aatgccatat cgtcctcctc aatttatctt tctccattat
    20701 atcacatctt tttcatttta aaaataaaaa ttcttgtgct acaatgaaat gagaaagaat
    20761 tgaggttatt tatggagatt tgccaacaaa ttttagaaaa aattaaagaa tacgacacca
    20821 ttatcattca tcgtcatatg aaaccagacc ctgatgcctt gggaagtcag gtgggattga
    20881 aagccttgct ggaacatcat ttcccagaaa aaaccatcaa agccgtcggt tttgatgaac
    20941 caactcttac ttggatggct gagatggatc ttgttgaaga tagagcctac caaggcgcac
    21001 ttgtcatcgt ctgtgataca gctaatactg ctcgtatcga tgataagcgc tatagtcaag
    21061 gtgattttct cattaagatt gaccaccatc caaatgatga tgtatacggt gacctgtctt
    21121 gggtcgatac tagttcaagt agcgctagcg agatgattac cctatttgcc caaacaaccc
    21181 aactagcctt ggcagatcgc gatgctgagt tgctctttgc aggaattgtc ggtgatacag
    21241 gtcgcttcct ctacccttct accactgcac ggactcttcg cctggctgct tatttgagag
    21301 aacataactt tgactttgcg gctctcactc gcaaaatgga cactatgagc tacaaaattg
    21361 ctaaactgca aggctacatc tacgaccatc tggaagtgga tgaaaatggt gctgctcgcg
    21421 ttatcctgag tcaagaagtc ttgaaacaat acaatgtaac cgatgctgaa actgcagcca
    21481 ttgtcggtgc acctggacgc attgacagag tgagtctctg gggaattttt gtcgaacagg
    21541 ctgatggcca ctaccgagtt cgcttacgca gtaaaatcca tcctatcaat gaaatcgcca
    21601 aggagcatga tggtggaggc caccctctag caagtggtgc taattcctat agcctagaag
    21661 aaaacgaaat catctaccaa aagttgaaaa acttgcttaa aaactgataa aatacttgcc
    21721 aaacttttca gaatctgata gactagtatg gtaacaatct atggctcgca aagagaccat
    21781 ggcagaaagg aaatattgca aaatgaaaaa agatatccat ccagaatatc gcccagttgt
    21841 cttcatggac acaactactg gttaccaatt ccttagcggt tcaacaaaac gctctaacga
    21901 aacagttgag ttcgaaggcg agacttaccc attgatccgt gtggaaattt catcagactc
    21961 acacccattc tacactggac gtcaaaagtt cactcaagca gatggacgcg tggatcgttt
    22021 caacaaaaaa tacggtctca aataatgata agagaacagt ttcggctgtt cttttttgtt
    22081 tcttgaaatc aactgctgtt ttcatgttcc agactcatct gtaggttcgg tttccatgct
    22141 actaggcagg aaggaaatag ctgtttcaac acgtccataa tgagctatac tattgtaacg
    22201 aaccacactt tcattgatgg tccaagtgga attcattttc ttaaaagctt ctcggacttt
    22261 ttccaaatct ttggaggcaa tggcctgctc taaggtttca aaacgaggac ttatactcat
    22321 ctgctttcac aaagcattct agtccatctc cgatccttgt atgacatctt gaagttgatt
    22381 catgatatct tccaaagttc gaaaggcctt attcttaaat ccacgtttac gaatctcttt
    22441 ccacacttgt tcaatgggtt catctctggt gtgtatggag gaataaaggt aaaaccaata
    22501 ttagtcggaa tctttaaggt acttgattta tgccatatag cattgtccat aacgagtaaa
    22561 aggataagct tgtgaaagct cttctaaaaa ggcgttcatc cacactcctt tttataagcc
    22621 tgaaataagg catcaattgt aacaaattct cctgcctctg tagccttcaa atgacgggca
    22681 agaaaggctt tctcttcctc aactgtcata tatgcatggt tacgaccacc acgtgtttct
    22741 tgaaggagag agtcgagtcc gaactcctca tatttt
//
LOCUS       DAVLVP010000021        22606 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.021, whole genome shotgun sequence.
ACCESSION   DAVLVP010000021 DAVLVP010000000
VERSION     DAVLVP010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.021"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(93..1073)
                     /locus_tag="VYN69_000822"
     CDS             complement(93..1073)
                     /locus_tag="VYN69_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylxylan esterase"
                     /protein_id="HEV1533123.1"
                     /translation="MKNPALLEEIKTYRGRDEVPEDFDDFWDGEVKNVSTLPSYHLEE
                     RDFHIPQVKCYELTFEGSKEGKVYARIVLPKSEEKVPLIFHFHGYMGRGWDWADMLGF
                     TVAGYGVVSMDVRGQSGYSQDGLRSPLGNTVKGHIIRGAVEGRDHLFYKDVYLDIYQL
                     VEIVASLSQVDEKRLSSYGASQGGALALVAAALNPRIQKTVAIYPFLSDFRRVIEIGN
                     TSEAYDELFRYFKFHDPFHETEEEIMATLAYIDVKNLAHRIQGEVKMITGLDDDVCYP
                     ITQFAIYNRLTCDKTYRIMPEYAHEAMNVFVNDQVYNWLCGSEIPFKYLK"
     gene            complement(1400..1603)
                     /locus_tag="VYN69_000823"
     CDS             complement(1400..1603)
                     /locus_tag="VYN69_000823"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533124.1"
                     /translation="MKIKEQTRKLTAGCSKHCFEVVDETDEVSSKHRFEVADRTDEVS
                     SKHCFEVADRTDEVSNIYTVRRR"
     gene            complement(1631..3646)
                     /gene="recG"
                     /locus_tag="VYN69_000824"
     CDS             complement(1631..3646)
                     /gene="recG"
                     /locus_tag="VYN69_000824"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001048793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEV1533125.1"
                     /translation="MNLHQPLHVLPGVGPKSAEKYAKLGIENLQDLLLYFPFRYEDFK
                     TKQVLELEDGEKAVLSGQVVTPASVQYYGFKRNRLRFSLKQGEVVFAVNFFNQPYLAD
                     KIELGATLAVFGKWDRAKASLTGMKVLAQVEDDLQPVYRLAQGISQASLVKVIKTAFD
                     QGLDLLIEENLPQSLLDKYKLMSRCQAVRAMHFPKYLAEYKQALRRIKFEELFYFQMQ
                     LQMLKSENRVQGSGLVLNWSQEKVTAVKVSLPFALTQAQEKSLQEILTDMKSDHHMNR
                     LLQGDVGSGKTVVAGLAMFAAVTAGYQAALMVPTEILAEQHFESLQNLFPNLKLALLT
                     GSLKAAEKREVLETIAKGEADLIIGTHALIQDGVEYARLGLIIIDEQHRFGVGQRRIL
                     REKGDNPDVLMMTATPIPRTLAITAFGDMDVSIIDQMPAGRKPIVTRWIKHEQLPQVL
                     TWLEGEIQKGSQVYVISPLIEESEALDLKNAIALSEELTTHFAGKAEVALLHGRMKSD
                     EKDQIMQDFKERKTDILVSTTVIEVGVNVPNATVMIIMDADRFGLSQLHQLRGRVGRG
                     DKQSYAVLVANPKTDSGKDRMRIMTETTNGFVLAEEDLKMRGSGEIFGTRQSGLPEFQ
                     VADIIEDFPILEEARKVASYISSIEAWQEDPEWRMIALHLEKKEHLD"
     gene            complement(3665..4768)
                     /locus_tag="VYN69_000825"
     CDS             complement(3665..4768)
                     /locus_tag="VYN69_000825"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000648075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEV1533126.1"
                     /translation="MKASPHRPTKALIHLGAIRQNIQQMGAHIPQGTLKLAVVKANAY
                     GHGAVAVAKAIQDDVDGFCVSNIDEAIELRQAGLSKPILILGVSEIEAVALAKEYDFT
                     LTVAGLEWIQALLDKEVDLTGLTVHLKIDSGMGRIGFREASEVEQAQDLLQQHGVCVE
                     GIFTHFATADEESDDYFNAQLERFKTILASMKEVPELVHASNSATTLWHVETIFNAVR
                     MGDAMYGLNPSGAVLDLPYDLIPALTLESALVHVKTVPAGACMGYGATYQADSEQVIA
                     TVPIGYADGWTRDMQNFSVLVDGQACPIVGRVSMDQITIRLPKLYPLGTKVTLIGSNG
                     DKEITATQVATYRVTINYEVVCLLSDRIPREYY"
     gene            complement(4758..5126)
                     /locus_tag="VYN69_000826"
     CDS             complement(4758..5126)
                     /locus_tag="VYN69_000826"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEV1533127.1"
                     /translation="MRMIVGHGIDIEELASIESAVTRHEGFAKRVLTAQEMERFTSLK
                     GRRQIEYLAGRWSAKEAFSKAMGTGISKLGFQDLEVLNNERGAPYFSQAPFSGKIWLS
                     ISHTDQFVTASVILEENHES"
     gene            complement(5165..6196)
                     /locus_tag="VYN69_000827"
     CDS             complement(5165..6196)
                     /locus_tag="VYN69_000827"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1533128.1"
                     /translation="MAFIEKGQEIDMEVIKAETQLSAEALRLKESRDRELADIISGED
                     DRILLVIGPCSSDNEEAVLEYARRLSALQKKVADKIFMVMRVYTAKPRTNGDGYKGLV
                     HQPDTSKAPSLINGLQAVRQLHYRVITETGLTTADEMLYPSNLILVDDLVSYHAVGAR
                     SVEDQEHRFVASGIDAPVGMKNPTSGNLGVMFNAIYAAQNKQTFLYHGQEVETSGNPL
                     AHVILRGAVNEYGNYMPNYYYENLLQAIERYETMGLENPFILIDTNHDNSGKQYMEQI
                     RIVRQTLQNRDWNEKIKKTVRGFMIESYLADGRQNQPEIFGCSITDPCLGWENTEALV
                     EEIYATLTK"
     gene            complement(6198..7229)
                     /locus_tag="VYN69_000828"
     CDS             complement(6198..7229)
                     /locus_tag="VYN69_000828"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000232189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEV1533129.1"
                     /translation="MVFAAKSSKINIEEVRALSKLEGQALERKSQRDQELEAIIRGED
                     QRILLVIGPCSSDNEEAVLEYAKRLAVLQEEVADRIFMVMRVYTAKPRTNGDGYKGLI
                     HQPNATEAPSLINGIKAVRHLHYRVITETGMTTADEMLYPENLPLVDDLISYMAVGAR
                     SVEDQQHRFVASGAGFSTGFKNPTSGNLNVMFNGIYAAQNKQSFLFLGKEVETTGNPL
                     SHAILRGALNEYGKNIPNYYYDNLIDTIAQYEKMGLENPFIIIDTNHDNSGKQYIEQI
                     RIVRQTLINRAWNEKIKQFVRGFMIESYLEDGRQNEPEVFGKSITDPCLGWDNTEALV
                     REIYKTLGE"
     gene            complement(7310..9823)
                     /gene="secA"
                     /locus_tag="VYN69_000829"
     CDS             complement(7310..9823)
                     /gene="secA"
                     /locus_tag="VYN69_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEV1533130.1"
                     /translation="MANILKTIIENDKGEIRRLEKMADKVFKYEDQMAALTDDQLKAK
                     TVEFKERYQNGESLDSLLYEAFAVVREGAKRVLGLFPYKVQVMGGIVLHHGDVPEMRT
                     GEGKTLTATMPVYLNALSGKGVHVVTVNEYLSERDATEMGELYSWLGLSVGINLATKS
                     PMEKKEAYECDITYSTNSEIGFDYLRDNMVVRAENMVQRPLNYALVDEVDSILIDEAR
                     TPLIVSGANAVETSQLYHMADHYVKSLNKDDYIIDVQSKTIGLSDSGIDRAESYFKLE
                     NLYDIENVALTHFIDNALRANYIMLLDIDYVVSEEQEILIVDQFTGRTMEGRRYSDGL
                     HQAIEAKEGVPIQDETKTSASITYQNLFRMYKKLSGMTGTGKTEEEEFREIYNIRVIP
                     IPTNRPVQRIDHSDLLYASIESKFKAVVEDVKARYQKGQPVLVGTVAVETSDYISKKL
                     VAAGVPHEVLNAKNHYREAQIIMNAGQRGAVTIATNMAGRGTDIKLGEGVRELGGLCV
                     IGTERHESRRIDNQLRGRSGRQGDPGESQFYLSLEDDLMKRFGSERLKGIFERLNMSE
                     EAIESRMLTRQVEAAQKRVEGNNYDTRKQVLQYDDVMREQREIIYAQRYDVITADRDL
                     APEIQSMIKRTIERVVDGHARAKQDEKLEAILNFAKYNLLPEDSITMEDLSGLSDKAI
                     KEELFQRALKVYDSQVSKLRDEEAVKEFQKVLILRVVDNKWTDHIDALDQLRNAVGLR
                     GYAQNNPVVEYQAEGFRMFNDMIGSIEFDVTRLMMKAQIHEQERPQAERHISTTATRN
                     IAAHQASMPEDLDLSQIGRNELCPCGSGKKFKNCHGKRQ"
     gene            complement(9969..10256)
                     /locus_tag="VYN69_000830"
     CDS             complement(9969..10256)
                     /locus_tag="VYN69_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000940707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533131.1"
                     /translation="MAHLKSFITRYSKVYIGLVLLIWLSFFFIPWDRAILGVRIDIFI
                     IQKVLLVFGILSILMALLSKKVSLFVFGLICCLSLWINLFITFAILPIFGN"
     gene            complement(10267..10917)
                     /locus_tag="VYN69_000831"
     CDS             complement(10267..10917)
                     /locus_tag="VYN69_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1533132.1"
                     /translation="MRLEIINGQKIYGKRPILNQLNLVFQSGKIYGLKGDNGSGKTVL
                     LKVLAGYIKLDKGKVLQDGKVYGIKNHYIQDAGILIEKVEFLSHLSLRENLELLRYFS
                     SKVTEKRIAYWIQYYDLQEFEDVEYRHLSLGTKQKMALIQAFISSPSILFLDEPMNAL
                     DEKSVRLTKQVILSYLKKENGLVILTSHISEDISDLCTDVLVVENGHIQYVKDIQS"
     gene            complement(10921..11562)
                     /locus_tag="VYN69_000832"
     CDS             complement(10921..11562)
                     /locus_tag="VYN69_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533133.1"
                     /translation="MFKNFNNILLKRKIVLLLRIVLMMILINHLLSTAVQKQDAVIFF
                     KRELISIFSYNDYSEANLEIPKLLLNLSLFMVGWLSIILLESDLADHYHHLIRYQSSS
                     FFDYTRKRLVVISKFFTQDLFVWFLGLLPLGIHFKTVALFFLLAQLMMLYLLLSYLIA
                     LISAGAGFSFLLYFLAFVGQEWMMDHIVTVYLLLLSLLVMLILSRLEEKFKKG"
     gene            complement(11555..12346)
                     /locus_tag="VYN69_000833"
     CDS             complement(11555..12346)
                     /locus_tag="VYN69_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533134.1"
                     /translation="MKIPLLTLARHKFVYVLLTLLFLALVYRDVLMTYFFFDIHAPDL
                     AKFDGQAIKNDLLKSALDFRILQFNLGFYQSFIIPIIIVLLGFQYIELKNKVLRLSIG
                     REVSYQGLKRKLTLQVASIPCLIYLVTVLTIAIITYFLGTFSPLGWNSLFSDGSGLQR
                     LLDGEIKSYLFFTCVLLIGIFINAIYFLQIVDYVGNVTRSAIAYLMFLWLGSILLYST
                     LPYYMVPMTSLMQASYGDVSLMKLFTPYILYVVPYMVLKKYEDNV"
     gene            complement(12531..12866)
                     /locus_tag="VYN69_000834"
     CDS             complement(12531..12866)
                     /locus_tag="VYN69_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000400498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533135.1"
                     /translation="MEHLFKFLLLAPYFYFDNWIEKANRNSKFFPIFYYFYWFYITLY
                     ALFSLAWTVFSVLFFNIVLRNLTDIKLWGIWLLLLLIAFASDWLAYVFFKKMIRLRRE
                     LGKSKGGRH"
     gene            complement(12957..14267)
                     /gene="der"
                     /locus_tag="VYN69_000835"
     CDS             complement(12957..14267)
                     /gene="der"
                     /locus_tag="VYN69_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018377046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEV1533136.1"
                     /translation="MALPTIAIVGRPNVGKSTLFNRIAGERISIVEDVEGVTRDRIYA
                     TGEWLNRSFSMIDTGGIDDVDAPFMEQIKHQAEIAMEEADVIVFVVSGKEGITDADEY
                     VARKLYKTHKPVILAVNKVDNPEMRNDIYDFYALGLGEPLPISSVHGIGTGDVLDAIV
                     ENLPNEYEEENPDVIKFSLIGRPNVGKSSLINAILGEDRVIASPVAGTTRDAIDTHFT
                     DTDGQEFTMIDTAGMRKSGKVYENTEKYSVMRAMRAIDRSDVVLMVINAEEGIREYDK
                     RIAGFAHEAGKGMIIVVNKWDTLEKDNHTMKNWEEDIREQFQYLPYAPIIFVSALTKQ
                     RLHKLPEMIKQISESQNTRIPSAVLNDVIMDAIAINPTPTDKGKRLKIFYATQVATKP
                     PTFVIFVNEEELMHFSYLRFLENQIRKAFVFEGTPIHLIARKRK"
     gene            complement(14281..14994)
                     /locus_tag="VYN69_000836"
     CDS             complement(14281..14994)
                     /locus_tag="VYN69_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent oxidoreductase"
                     /protein_id="HEV1533137.1"
                     /translation="MTETIKLMKAHTSVRRFKEQEIPQVDLNEILTAAQMASSWKNFQ
                     SYSVIVVRSQEKKDALYELVPQEAIRQSAVFLLFVGDLNRAEKGARLHTDTFQPQGVE
                     GLLISSVDAALAGQNALLAAESLGYGGVIIGLVRYKSEEVAELFNLPDYTYSVFGMAL
                     GVPNQHHDMKPRLPLENVVFEEEYQEQSTEAIQAYDRVQADYAGARATTSWSQRLAEQ
                     FGQAEPSSTRKNLKQKKLL"
     gene            complement(14991..15887)
                     /gene="dnaI"
                     /locus_tag="VYN69_000837"
     CDS             complement(14991..15887)
                     /gene="dnaI"
                     /locus_tag="VYN69_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEV1533138.1"
                     /translation="MESVGDVLKRQPSRFYYQDLVQKIMKDPDVAAFIQQESLTPEEL
                     NRSISKFNQYITERDKFLRGDTDYIAKGYKPILVKNHGYADVSYEETPELIAAEKEAA
                     IKNRLKLINLPASLKKASLAQVDLDDLGRLPVFEKLLAFVEQYPAIRKGLYLYGDFGV
                     GKSFMVAALAHDLSEKRGVSSTLLHYPSFVIDVKNAISDGNVKTLVDEIKLSEVLILD
                     DIGAEQSTAWVRDEILQVILQYRMQENLPTFFTSNFNFEDLEKHFAKVKHGNDETWEA
                     RRVMERIRYLAEETRLEGVNRR"
     gene            complement(15888..17057)
                     /locus_tag="VYN69_000838"
     CDS             complement(15888..17057)
                     /locus_tag="VYN69_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000802368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HEV1533139.1"
                     /translation="MKPIDRFSYLKNNRVSQDTSSLVQCYLPIIGQEALSLYLYTISF
                     WDNGRKEYLFSSILNHLNFGMDRLIKSLKILSAFNLLTLYQKGDVYQLALHAPLSSQD
                     FLEHPVYRRLLEKKIGDSAVEDLKVESADGEEIPVSLNQVFPELAELGSQEDLGLKKK
                     VANDFDLEHFRQLMARDGLRFADEQSDVLNLFAIAEEKKWTWFETYQLAKSTAVSQVI
                     STKRMREKIAQKPVSSDFSLKETTIIKEAKSKTALQFLAEIKQTRKGTITQTERELLQ
                     QMAGLGLLDEVINIILLLTFNKVDSANINEKYAMKVANDYAYQKIHSAEKAVLRIRER
                     GQKAKTQKQNQTAPVKTNVPKWSNPEYKNETSEETRLELERKKQELLARLEKGGD"
     gene            complement(17058..17531)
                     /gene="nrdR"
                     /locus_tag="VYN69_000839"
     CDS             complement(17058..17531)
                     /gene="nrdR"
                     /locus_tag="VYN69_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001203675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEV1533140.1"
                     /translation="MRCPKCGATKSSVIDSRQAEEGNTIRRRRECDECQHRFTTYERV
                     EERTLVVVKKDGTREQFSRDKIFNGIIRSAQKRPVSSDEINMVVNRIEQKLRGRNENE
                     IQSEDIGSLVMEELAELDEITYVRFASVYRSFKDVSELESLLQQITQSSKKKKER"
     gene            17671..18036
                     /locus_tag="VYN69_000840"
     CDS             17671..18036
                     /locus_tag="VYN69_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1533141.1"
                     /translation="MSWTFDNKKPIYLQIMEKIKLQIVSHTLEPNQQLPTVRELASEA
                     GVNPNTIQRALSDLEREGFVYSKRTTGRFVTKDKELIAQSRKQLSEEELEHFVSSMTH
                     FGYEKEELPGVVSDYIKGV"
     gene            18041..18736
                     /locus_tag="VYN69_000841"
     CDS             18041..18736
                     /locus_tag="VYN69_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533142.1"
                     /translation="MSLLAFENVSKSYGATPALENVSLDIPAGKIVGLLGPNGSGKTT
                     LIKLINGLLQPDQGRVLINDIDPSPATKAVVAYLPDTTYLNEQMKVKEALTYFKTFYK
                     DFNLERAHHLLADLGIDENSRLKKLSKGNKEKVQLILVMSRDARLYVLDEPIGGVDPA
                     ARAYILNTIINNYSPTSTVLISTHLISDIEPILDEIVFLKDGKVVRQGNVDDIRYESG
                     ESIDQLFRQEFKA"
     gene            18753..19520
                     /locus_tag="VYN69_000842"
     CDS             18753..19520
                     /locus_tag="VYN69_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533143.1"
                     /translation="MFWNLVRYEFKNVNKWYLALYAAVLVLSALIGIQTQGFKNLPYQ
                     ESQATMLLFLATVFGGLMLTLGISTIFLIIKRFKGSVYDRQGYLTLTLPVSEHHIITA
                     KLIGAFIWSLISTAVLALSAVIILALTAPEWIPLSYVITFVETHLPQIFLTGISFLLN
                     TISGILCIYLAISIGQLFNEYRTALAVAVYIGIQIVIGFIELFFNLSSNFYVNSLVGL
                     NDHFYMGAGIAIVEELIFIAIFYLGTYYILRNKVNLL"
     gene            complement(19873..21072)
                     /locus_tag="VYN69_000843"
     CDS             complement(19873..21072)
                     /locus_tag="VYN69_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1533144.1"
                     /translation="MRNMWVVIKETYLRHVESWSFFFMVISPFLFLGISVGIGHLQGS
                     SMAKNNKVAVVTTVPSVAEGLKNVNGVNFDYKDEASAKEAIKEEKLKGYLTIDQEDSV
                     LKAVYHGETSLENGIKFEVTGILNELQNQLNRSTASLSQEQEKRLAQTIQFTEKIDEV
                     KENKKFIQTIAAGALGFFLYMILITYAGVTAQEVASEKGTKIMEVVFSSIRASHYFYA
                     RMMALFLVILTHIGIYVVGGLAAVLLFKDLPFLAQSGILVHLGDAISLNTLLFILISL
                     FMYVVLAAFLGSMVSRPEDSGKALSPLMILIMGGFFGVTALGAAGDNLLLKIGSYIPF
                     ISTFFMPFRTINDYAGGAEAWISLAITVIFAVVATGFIGRMYASLVLQTDDLGIWKTF
                     KRALSYK"
     gene            complement(21069..21962)
                     /locus_tag="VYN69_000844"
     CDS             complement(21069..21962)
                     /locus_tag="VYN69_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533145.1"
                     /translation="MLEVRSLEKSFGSKQVLFGIDFQARPGRILGLVGKNGAGKTTIF
                     HSILKFLEYQGEIGLDGQDIRQETYARIGYLPEERSLMPKLTVLEQVRYLATLKGMDA
                     KEVKEKLPQWMKRLEVKGKLTDKIKSLSKGNQQKIQLIITLIHEPDLIILDEPFSGLD
                     PVNTELLKQVIFQEKERGATIIFSDHVMTNVEELCDDILMIRDGRVVLHGPVQDVRNQ
                     YGKTRLFVSSERSKEELENLPHVKQVSLTKQGSWKLILEDESAGRELFPILTQGQYIA
                     TFDQQAPTIDEIFKLESGVEV"
     gene            22078..22158
                     /locus_tag="VYN69_000845"
                     /pseudo
     CDS             22078..22158
                     /locus_tag="VYN69_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
BASE COUNT         6580 a         5099 c         4219 g         6708 t
ORIGIN      
        1 tccatgataa aacgcatagt ataaagtttt cttaatcccc tgatactatg cgtgtttgta
       61 atttttaaga ttttgtgctt agagtcgact ccttatttta gatatttaaa aggaatctca
      121 cttccacaga gccagttgta gacttggtca ttgacaaata cattcatggc ttcgtgagca
      181 tactcaggca tgatgcgata ggttttatcg caggtcagac gattataaat cgcaaactgg
      241 gtaatgggat agcaaacatc gtcgtccaag cccgtaatca tcttaacctc accttggata
      301 cgatgggcaa gatttttgac atcgatatag gcaagggtcg ccatgatttc ctcctctgtt
      361 tcatggaagg ggtcgtgaaa cttgaaataa cggaaaagtt cgtcgtaagc ctcgctagta
      421 ttaccaatct caatcacccg tctgaagtct gacaagaagg gataaatggc aactgttttc
      481 tgaattcgag gattgagcgc tgctgcaact agagctagag cccctccttg tgaggcacca
      541 tagctagaaa gacgcttctc atcaacctga gacagactag caacaatttc gaccaactgg
      601 taaatatcca gataaacatc cttataaaag aggtggtccc gaccttccac agcaccacgg
      661 ataatatgcc ccttcacggt atttcctaaa ggagaacgca agccgtcttg tgagtaacct
      721 gactggcccc gcacatccat ggaaacaaca ccgtaaccag ctacggtgaa gcccagcatg
      781 tcggcccagt cccagccacg tcccatataa ccatgaaaat ggaagattaa tggaaccttc
      841 tcctcactct ttggaagaac aatgcgtgca tagacctttc cttccttgct tccttcaaat
      901 gttaactcat agcacttgac ttgaggaatg tggaaatctc tttcctccaa gtggtaggat
      961 ggaagcgtgg aaacattttt cacttcccca tcccagaaat catcaaagtc ttccggaacc
     1021 tcatcccttc ctctataggt cttaatttct tctagcaaag ctggattttt catagcatgc
     1081 tccttttatt ttgtaatcgt taccacaact gtagcatatc tatgtaatca atgcaagaaa
     1141 gaagggtaaa tagaaagtga atttagattc tttactttta ggatgatggg ctgaaagtag
     1201 ttttagaaac gaaaaaagag catttcgctc tctcttctgt ttatactagt tttttgcttc
     1261 cttcttttgg aaagtggttt ggtaatttac tttaatcgtt actgtcatat gagagtcctc
     1321 gtttcttttt gatgactcta gtataagagc caaacctaaa aactagctta atactcttcg
     1381 aaaatctctt caaaccacgt cagcgtcgcc ttaccgtata tatgttactg acttcgtcag
     1441 ttctatctgc cacctcaaaa cagtgttttg agctgacttc gtcagttcta tctgccacct
     1501 caaaacggtg ttttgagctg acttcgtcag tttcatccac aacctcaaaa cagtgttttg
     1561 agcaacctgc ggttagcttc ctagtttgct ctttgatttt cattgagtat aagttttcct
     1621 tagagaaagc ttaatccaga tgttctttct tttccagatg aagggcaatc atgcgccact
     1681 ctggatcttc ttgccaagct tctatagaac taatgtagct agcaaccttt cttgcttctt
     1741 ctaaaatcgg aaaatcttcg ataatatcag ccacttggaa ctctggaagt cctgactgtc
     1801 tggttccaaa aatctcacca gaaccacgca ttttcaaatc ttcctccgca aggacaaatc
     1861 cattggtcgt ttctgtcatg atacgcatgc ggtctttccc agaatccgtc ttgggattag
     1921 caacgagaac agcgtaggac tgcttgtccc cccgaccgac acgacctcta agctggtgaa
     1981 gttgactgag accgaagcga tcggcatcca tgataatcat gacagtcgca ttgggaacgt
     2041 tgaccccaac ctcaataacc gtcgtcgaaa ccagaatatc cgtctttctc tccttgaaat
     2101 cctgcatgat ctggtctttt tcgtcactct tcatcctacc atgtagaaga gccacctctg
     2161 ccttgcctgc aaaatgagtc gtcaactcct ctgataaggc aatggcattt ttcaaatcta
     2221 gagcttctga ttcttcaatc aaaggagaga tgacatagac ttgggaacct ttttgaattt
     2281 ccccctctaa ccaagtcaag acctgaggta gttgctcatg tttgatccag cgcgtcacaa
     2341 taggcttccg acctgctggc atctggtcga taatggaaac atccatatct ccaaaggctg
     2401 tgatggcaag cgtccgtgga atgggagtcg ccgtcatcat gaggacatct ggattgtcac
     2461 ctttttcccg taaaatacgc ctttgcccta caccaaaacg gtgctgctca tcgataataa
     2521 tcaaaccaag acgagcatac tccaccccat cttgtatcag agcgtgagtt cctataatca
     2581 aatcagcctc acccttggca atggtctcca agacttctct cttttctgca gctttcaagg
     2641 aacctgtcaa gagagccagt ttcaaattgg gaaaaaggtt ctgtaaactc tcaaagtgtt
     2701 gctctgcgag gatttctgtt ggtaccatta gggcagcctg ataacctgct gtcactgccg
     2761 caaacatggc caagccagcg actaccgttt ttccactccc cacatcccct tgtaggagac
     2821 gattcatgtg gtggtcggac ttcatatcag ttaaaatttc ctgcaaactc ttttcctgag
     2881 cttgggtcag ggcaaaagga agacttactt taactgctgt cactttttcc tgagaccaat
     2941 tcagaaccag accacttccc tgaactctat tttcagactt gagcatctgc agctgcattt
     3001 ggaaataaaa gagttcctca aactttatac ggcgaagagc ctgcttgtat tctgccaaat
     3061 actttggaaa atgcatagca cggactgcct gacaacggga catgagtttg tatttgtcta
     3121 gtaaagactg gggcagattt tcttctatca agaggtccag tccctgatca aaagccgtct
     3181 tgatgacctt gaccagactg gcctgactga ttccctgagc cagacgatag acaggctgga
     3241 ggtcatcttc tacctgagcc agaaccttca tcccagtcag actagcctta gcgcggtccc
     3301 attttccaaa gacagcaagg gttgctccca actctatttt atcagccaga tagggctggt
     3361 taaagaaatt caccgcaaaa acgacctctc cctgcttgag actaaaacgc aggcgattgc
     3421 gcttgaaacc ataatactgg acactagcag gagtcactac ctgaccagaa agaactgcct
     3481 tctcaccgtc ttccagctcc agcacctgct tggttttgaa gtcttcataa cggaaaggaa
     3541 agtagagcaa gagatcttgc aagttttcaa ttcctagttt ggcgtatttt tctgctgact
     3601 ttggtcccac accaggcaag acatgcaagg gttgatgtag attcatgctc cactcctttc
     3661 ttttctaata atattctctc ggaatacggt cgctgaggag gcaaaccacc tcatagttaa
     3721 tggttacgcg gtaggtcgct acctgagttg cagtgatttc cttatcccca ttggagccaa
     3781 tcaaggttac cttggttcct agcggataaa gcttaggcaa tcgaatagtg atttggtcca
     3841 tcgaaaccct gccgacaatt gggcaagctt ggccatctac caagacagag aaattttgca
     3901 tgtctcttgt ccatccatct gcatacccga ttggcacggt cgcgatgact tgctcgctat
     3961 ccgcttgata agttgctcca tagcccatgc aagctccagc tggaactgtc ttgacatgaa
     4021 ccagagcaga ctccaaggtc aaggccggta tcaaatcata aggcaaatcc aagaccgctc
     4081 cacttggatt gaggccatac atggcatctc ccatacgaac cgcattgaaa atagtctcta
     4141 catgccaaag agtcgttgca gaattgctag catgaaccag ctctggaact tccttcatac
     4201 tagctaaaat agttttaaac cgttctaact gggcattaaa atagtcatct gattcctcat
     4261 cagcagtagc aaagtgggta aagattcctt caacacaaac accgtgttgt tggagcaaat
     4321 cttgagcctg ctcaacctca cttgcctctc taaaaccaat ccgtcccatc cctgaatcaa
     4381 tcttgaggtg gactgtcaat ccagttaggt ccacttcctt atctaagagt gcttgaatcc
     4441 actccagtcc agccactgtc aaggtgaagt catattcttt agctagagca acagcttcga
     4501 tttcagaaac tcctaaaatg aggattggct tgctgagtcc agcttgtctg agttcaatgg
     4561 cttcatcgat attggaaacg caaaagccat caacatcatc ttgaattgcc ttggcaacgg
     4621 caacagctcc atgaccataa gcattggcct taaccacagc caacttgagc gttccttgag
     4681 ggatatgagc ccccatttgc tgaatatttt gtcgaatagc tcccagatga atcagagcct
     4741 tggttggtct atgtggacta gctttcatga ttttcctcca aaatgacact ggctgtcaca
     4801 aactgatcgg tgtggctgat agacagccaa atctttcctg aaaatggtgc ctgactaaaa
     4861 taaggcgccc cacgttcatt gttcaagact tccaaatcct gaaaaccgag cttgctaatg
     4921 cccgttccca tagccttgga aaaggcctcc ttagccgacc agcgaccagc taaatattct
     4981 atttgcctgc gtcctttgag actggtgaag cgctccattt cctgagcggt cagtacacgc
     5041 ttagcaaatc cttcatgtcg tgtaactgcg ctttctatcg aagccaattc ttcgatgtca
     5101 attccgtgtc caactatcat tctcatcaaa aggagttgag attccccaac tccatccttt
     5161 tcacttattt tgtcaaggta gcatagattt cttctaccaa ggcctctgta ttttcccaac
     5221 ctaggcaagg gtcagtaata gagcaaccaa agatttctgg ttggttttga cgaccatctg
     5281 ctaggtaaga ttcaatcata aatcctcgaa ccgtcttttt aattttctca ttccaatcac
     5341 gattctgcaa ggtctggcga acaattcgaa tctgctccat atattgcttg cctgagttat
     5401 catggttggt gtcaatgagg ataaaaggat tttcaagtcc catggtttca tagcgttcaa
     5461 tggcttggag taggttttca tagtagtaat tcggcatata attgccatac tcgttgactg
     5521 ctccacggag gataacatgg gccaaaggat tacctgatgt ctcaacttcc tgcccatgat
     5581 aaaggaaggt ttgcttgttt tgagcagcat agatggcgtt aaacataaca cccaaatttc
     5641 ctgaggttgg atttttcatc cctactggtg catcaatccc agaagccaca aagcggtgct
     5701 cttggtcttc cacagaacga gctccaacgg catggtagct gaccaagtca tccaccaaga
     5761 tcagatttga cggataaagc atctcatctg ccgttgtcaa accagtctct gtaatcacgc
     5821 ggtagtgcaa ctggcgcaca gcctgcaagc cattaatcag gcttggagcc ttagaagtat
     5881 ctggctggtg aaccaatcct ttatagccgt ctccattggt acgaggctta gcagtataca
     5941 cgcgcatgac catgaaaatc ttgtccgcta ccttcttttg caaggcggat aaacggcgag
     6001 catattccaa gaccgcctct tcattatcag aagagcaagg accaatcacc aagagaatac
     6061 ggtcatcttc ccctgaaata atatctgcca attccctgtc acggctttcc ttgagtctca
     6121 aggcttccgc agacaattgg gtttcagcct tgatgacttc catatcgatt tcttgacctt
     6181 tttcaataaa tgccatctta ttctcctaac gttttgtaaa tttctctgac aagagcttct
     6241 gtgttatccc aacccaggca agggtctgtg atagacttac caaatacttc tggctcattt
     6301 tgtcgaccat cttccagata agactcaatc ataaaaccac gaacgaactg cttaattttt
     6361 tcattccaag cacggttaat caaggtctgg cggacaattc ggatctgttc aatatactgc
     6421 ttaccagaat tgtcatgatt ggtatcaatg atgataaaag gattttccaa gcccattttc
     6481 tcatactggg caatggtatc aattaaattg tcataatagt agttgggaat attttttcca
     6541 tactcattaa gagcaccacg aagaatagcg tgtgaaagcg ggttcccagt tgtttctact
     6601 tcttttccta agaaaaggaa actttgtttg ttttgagcag cataaatccc attaaacatg
     6661 acattgagat ttccagaggt tggattttta aaaccagtag aaaatcctgc cccacttgcc
     6721 acaaagcggt gttgctggtc ttcaactgaa cgggcaccaa ctgccatgta agaaatcaaa
     6781 tcatctacaa gcggaaggtt ttcaggataa agcatttcat cagcagttgt catccctgtt
     6841 tctgtgatga cacgatagtg aagatggcga acggctttga ttccattgat aagactaggc
     6901 gcttctgtcg cgttaggctg gtgaatcaag cccttatagc catctccgtt ggtacggggt
     6961 ttggcagtat aaacacgcat aaccataaag atacgatctg ccacttcttc ttgtaggact
     7021 gccaaacgct tagcgtattc aaggacagct tcttcgttgt cagatgagca tggcccgatt
     7081 accaagagaa ttcgctggtc ttctccacgt ataatggctt ctagctcttg atctcgctgt
     7141 gatttcctct ccaaagcctg accttctaat tttgacaagg cacgaacttc ttctatattt
     7201 attttagagc tttttgctgc aaataccata actcatctcc ttataaagca aacggatacc
     7261 cagtaaaaat ttacttttca caagatatcc gcctctaaac tatctcattt tattgtcttt
     7321 taccgtgaca gtttttaaat ttcttaccag aaccacatgg gcaaagttca ttgcgtccaa
     7381 tctggctcaa atccaaatct tctggcatac ttgcttggtg agcagcgata ttgcgagtcg
     7441 ctgttgtact gatatgacgt tctgcctgtg gtctttcttg ttcatgaatt tgtgctttca
     7501 tcatcaagcg tgtcacatca aactcaatcg aaccaatcat atcattaaac atacggaaac
     7561 cttctgcctg atactcaaca acagggttgt tctgagcata gccacgaagt ccaaccgcgt
     7621 tacgcaattg atcaagggca tcgatatgat ctgtccactt gttatccacc actcgtagaa
     7681 tcaaaacttt ttggaattct ttaactgctt cttcatcgcg tagttttgaa acctgactat
     7741 cgtaaacctt caaggcacgt tggaaaagct cttccttgat ggccttatca gacaagcctg
     7801 acaagtcttc catcgtaata gaatcttcag gaagcaagtt gtacttagca aagttcaaaa
     7861 ttgcctctag tttttcatct tgtttggcac gcgcatgacc atcaacgaca cgttcaatcg
     7921 tgcgtttgat catagactga atttcaggtg ccaagtcacg atctgcagtg atgacatcgt
     7981 aacgttgagc atagataatc tcacgttgtt cacgcatgac atcatcgtat tgaaggactt
     8041 gtttacgggt atcgtagtta tttccttcga cacgtttctg agctgcttca acctgacgcg
     8101 tcaacatgcg agactcaatc gcctcttcag acatgttcaa gcgttcaaag attcccttca
     8161 agcgttcaga accaaaacgt ttcatcaaat catcttcaag agataggtag aattgtgact
     8221 cacctggatc tccttgacga cctgaacgtc cacgaagctg gttatcgata cgacgacttt
     8281 catgacgttc tgtaccaata acacaaagtc ctccaagttc acgaacacct tcaccaagct
     8341 tgatgtcggt accacgaccc gccatgttgg ttgcgatggt aacggcacca cgttgaccag
     8401 cattcatgat gatttgggct tctctatagt ggtttttggc attcaagact tcgtgaggaa
     8461 caccagctgc aaccaatttc ttagaaatgt agtcactagt ttcaaccgct actgtaccaa
     8521 ccaagacagg ttgacccttt tggtaacgag ccttaacgtc ttcgacaacc gctttaaact
     8581 tagattctat acttgcataa agaaggtctg agtggtcaat acgttgaaca ggacggtttg
     8641 ttgggattgg aataacacga atgttgtaga tttcacgaaa ttcttcttcc tcagtcttac
     8701 ctgtacccgt cataccagac aattttttgt acatacggaa aaggttttgg tacgtgattg
     8761 aggcagatgt cttggtttca tcctggattg gcacaccttc tttggcttca atagcttggt
     8821 gcaatccatc agaataacga cgaccttcca tggtacgacc tgtaaattgg tcgacaatca
     8881 agatttcttg ctcttcgctc accacatagt caatatcgag aagcatgatg tagttggcac
     8941 gaagggcgtt atcgataaag tgggtcaaag ccacgttttc gatgtcatag aggttttcaa
     9001 gtttgaagta gctttcagcc ctgtcaatcc ctgaatcaga caaaccaata gtcttagact
     9061 gcacatcgat gatgtagtca tctttgttca aagattttac atagtggtct gccatgtgat
     9121 acaactgact ggtttcaacc gcattggcac ctgatacaat caaaggtgta cgagcctcgt
     9181 caatcaagat agagtcaacc tcatcgacca aggcatagtt aagcggacgt tgtaccatgt
     9241 tttcagcgcg aacgaccata ttgtcacgaa ggtagtcaaa tccgatttct gagttagttg
     9301 agtaagtaat atcacactca taggcttctt ttttctccat tggagatttg gtagccaagt
     9361 taatccctac tgacaaacca agccaagagt acaattcacc catctcagtc gcgtcacgtt
     9421 ctgacaggta ttcattaacc gtaactacgt gaaccccttt acctgaaagg gcattgaggt
     9481 ataccggcat ggtcgcagtc aaggttttcc cttcccctgt acgcatctct ggcacgtcac
     9541 catggtgaag aacaatcccc cccatgacct gaaccttata agggaagaga cctaggacac
     9601 gtttggcacc ttcacggaca accgcaaatg cttcgtaaag caatgaatcc agtgattctc
     9661 cattttgata acgttcctta aattcaactg tttttgcttt tagttggtcg tcagtcaaag
     9721 cagccatttg gtcttcgtat ttgaaaacct tgtcagccat cttttccaga cgacggattt
     9781 ctcctttatc attttcgata attgttttta aaatattagc catgtttttc cttactttaa
     9841 attccgaata ttttagaatg ttcctttaat tttagcacaa ttactgatta ttttcaagga
     9901 agaatcccca ttttgaaaag gaaaaagagg ttagttttca agctaacctc tccgactttt
     9961 atgactgttt aattgccaaa aatcggcaaa atggcaaatg tgataaataa gttaatccaa
    10021 agagaaagac agcaaatcag tccaaaaaca aagagactga ctttcttgga cagcaaggcc
    10081 atcaaaatgg acagaattcc aaaaactagc aagactttct gtatgatgaa gatgtcaatc
    10141 cttaccccca gaatcgctct atcccaaggg ataaagaaga aagacagcca gatcagcaga
    10201 actaaaccaa tataaacctt ggaatagcgt gtaataaatg attttagatg tgccataagc
    10261 catctcctag gattgtatat cctttacata ttgtatatgt ccattttcga caactaatac
    10321 atctgtacaa aggtctgaaa tatcttccga tatgtgcgac gtcaggataa ccagaccatt
    10381 ttcttttttc aggtaagata aaatgacctg tttggttaac ctcacacttt tctcatccaa
    10441 agcattcata ggttcatcga gaaagagtat agaaggagag gaaataaagg cttgaatcaa
    10501 ggccattttt tgctttgttc ccaaggataa atgccggtat tcaacgtctt caaattcctg
    10561 taaatcatag tattgaatcc aataggcaat tcttttttcc gtaactttag atgaaaaata
    10621 ccttaacagt tccaaatttt ctctcaggga taaatgagat aaaaactcga ctttttcaat
    10681 taaaattcct gcatcctgaa tataatgatt ttttatcccg tagactttcc cgtcttgaag
    10741 aacttttcct ttgtcaagct taatataacc agcaagtacc tttaaaagaa ccgtcttgcc
    10801 agatccatta tcacctttaa gtccataaat ttttcctgat tgaaacacca aattcaactg
    10861 atttaaaata ggtcttttcc cataaatttt ctgtccattt ataatttcaa gtctcatcgt
    10921 ttatcctttc ttaaatttct cttccaagcg actaagaatc aacataacta ataaacttaa
    10981 gagtagtaaa tacactgtta caatatgatc catcatccat tcttgtccca caaatgctaa
    11041 aaaatagagt aaaaaggaaa aaccagcgcc cgcactaatc agtgctatca gataagacag
    11101 tagtaagtac aacatcatta actgagcaag taaaaagaaa agtgcgactg ttttgaaatg
    11161 aattcctaga ggaagtaaac caagaaacca gacaaacaaa tcttgagtaa aaaatttaga
    11221 aatgacaacc aatcgtttcc ttgtataatc gaaaaaggag cttgattgat agcgaatcaa
    11281 gtgatggtaa tggtctgcca aatcactttc aagtaaaatg atagagagcc atcctaccat
    11341 gaaaagcgaa aggtttaaca agagtttggg gatttctaaa ttcgcttcag aatagtcatt
    11401 ataggaaaaa attgaaatca attctctctt gaaaaagata acagcatcct gcttttgaac
    11461 cgctgttgac aatagatggt ttatcaaaat catcatcaga actatacgaa gtagtaaaac
    11521 aatctttctc tttagcaaaa tattgttaaa attcttaaac attatcttca tattttttaa
    11581 gtaccatata agggacaaca taaaggatat aaggagtaaa gagtttcatc aaacttacat
    11641 ccccatagct agcttgcatc aaactcgtca taggaaccat atagtaaggc aaggtactat
    11701 agagtaatat agaaccaagc caaagaaaca tcaaataggc gattgccgaa cgagtcacat
    11761 tccccacata atcaactatt tgtaaaaaat agattgcatt gatgaaaata ccgattagta
    11821 ggacacaagt aaagaacaaa tagcttttta tctctccatc taggagtctt tgtaaaccac
    11881 ttccatcaga aaatagagaa ttccatccaa gaggagaaaa agtcccaagg aaataggtta
    11941 taattgcaat tgtcagcaca gtcactaaat atatcaaaca agggatactt gcaacttgca
    12001 aagtcaactt tctttttaac ccttgatagc tcacttctct tccaatactc aatcgtaaaa
    12061 ctttattttt cagctcaata tattgaaaac ctagcaaaac aatgatgatt ggaataataa
    12121 atgattgata aaaacctaga ttgaactgga gaatacgaaa atctaatgct gattttaata
    12181 agtcattttt aattgcttgt ccatcgaatt tagctagatc gggcgcatga atatcaaaaa
    12241 agaaataagt catcaaaacg tcacgataaa ccaaagcaag aaaaagcaaa gtaagtaaga
    12301 cataaacaaa tttatgcctt gccaaagtta agagaggaat cttcataagc cacctcctac
    12361 aaatagaaag taatataata ctcaatgaaa atcaaagagc aaactaggaa actagccgca
    12421 agttgctcaa agcactgctt tgaggttgta gataagactg acgaagtcag ttacatatat
    12481 aatccaaggc gacgttgacg tggtttgaag agattttcga agagtataaa tcaatgcctt
    12541 ccgcctttag atttccctag ttcccgcctc aagcgaatca tttttttgaa aaagacatag
    12601 gctaaccaat cactagcaaa agcgataagt agtaataata gccaaatacc ccataacttg
    12661 atatctgtca aatttctcaa gacgatattg aaaaacagaa ctgaaaaaac tgtccaagca
    12721 aggctaaaaa gagcatagag ggtgatgtaa aaccagtaaa aataataaaa aattggaaaa
    12781 aacttactat ttctgttggc cttctcaatc cagttatcaa aataaaagta cggtgctaaa
    12841 agtaagaatt taaacaaatg ttccatcacc gacatcaccc tctttctttt gatagcgttt
    12901 tctattattt tattatatca aaaaaatccg gaactgtcat tccagattct acttttttat
    12961 ttgcgttttc ttgcgatgag atgaatcggt gttccctcaa aaacaaaggc cttgcggatt
    13021 tgattttcca agaaacgcag gtaagaaaag tgcatgagtt cttcttcatt gacaaagatg
    13081 acaaaggttg gtggtttggt tgccacttgg gtcgcataga aaatcttgag acgttttcct
    13141 ttgtctgtcg gtgttgggtt gatggcaatg gcatccatga tgacatcgtt caagacagct
    13201 gatggaatac gtgtattttg actttcgctg atttgcttaa tcatctcagg aagtttgtgg
    13261 agacgttgct tggttaaagc tgatacaaag ataatcggtg cgtaaggcag gtattggaac
    13321 tgctcacgga tatcttcttc ccagtttttc atagtgtggt tatctttttc aagcgtatcc
    13381 cacttgttga ccacgataat catcccttta ccagcttcat gggcaaatcc tgcgatacgc
    13441 ttgtcgtact cacgaatgcc ttcttccgca ttgatgacca tcaagaccac atctgaacgg
    13501 tcaatagcac gcatggcacg cataacagag tatttctcag tattttcata aaccttacca
    13561 gacttacgca taccagccgt atcaatcatg gtaaactctt gaccatctgt atctgtaaag
    13621 tgggtatcaa tggcatcacg agttgttcca gcaacaggac tagcaataac acggtcttct
    13681 cccaagatag cattgatcaa gcttgatttt ccaacgttag gacgaccaat caagctaaac
    13741 ttaatgacat ctggattttc ttcctcatat tcatttggaa gattttctac gatcgcatct
    13801 agcacatccc ctgtaccgat tccatggaca gatgagatag gcagtggttc acccaaaccg
    13861 agagcataga aatcatagat atcatttctc atctcagggt tgtccacctt gttgactgcg
    13921 aggataactg gtttgtgggt cttataaagc ttacgagcta cgtattcgtc tgcatcagta
    13981 attccttcct taccagacac gacaaaaacg ataacatctg cttcttccat ggcaatttct
    14041 gcctggtgct tgatttgttc catgaaagga gcatctacat cgtcgattcc tcctgtatca
    14101 atcatactaa aagagcgatt gagccactca cccgttgcat agatacggtc acgtgtcact
    14161 ccttcgacat cttctacaat ggagattcgc tcaccagcga tccgattaaa tagggttgat
    14221 ttcccaacat tgggacgtcc tacaatggca atagttggta gggccataat ttctcacttt
    14281 ctacaataac ttcttctgtt taagattttt tctagttgag cttggttctg cttggccaaa
    14341 ctgttctgct agacgctgac tccagcttgt ggtcgcacgc gctccagcat agtcagcctg
    14401 cacacggtca taagcttgga ttgcctcagt tgactgttct tggtattctt cctcaaagac
    14461 aacattctct agtggcagtc tcggtttcat atcatgatgt tgatttggca cacccagtgc
    14521 catcccaaag acagaatagg tgtagtcagg taggttaaag agctctgcca cttcttcaga
    14581 cttgtatcga accaaaccga taatcacacc accatagccc aagctttcag ctgccaacaa
    14641 ggcgttttgt ccagcaagag ctgcatcgac cgaactaatc aagagacctt ccacaccttg
    14701 gggttggaag gtgtcggtat gaagtctggc tcccttttct gctcggttca aatctccgac
    14761 aaagagaagg aaaacagcag actggcgaat ggcttcttga ggtaccaatt catacaaggc
    14821 atctttcttc tcttgacttc gtaccacaat cacagagtag gattggaaat tcttccaaga
    14881 tgatgccatc tgggctgctg tcaaaatctc atttaagtct acttggggaa tttcttgctc
    14941 tttaaacctg cgcactgaag tatgagcctt catcaattta atggtttctg tcatcgacgg
    15001 tttactcctt ctaaacgagt ctcctcagcc aaataacgga tgcgttccat gacccgtctg
    15061 gcttcccagg tttcgtcatt tccatgtttc actttcgcaa aatgcttctc caaatcttca
    15121 aagttgaagt tggatgtgaa aaaggtcggt aaattttcct gcatccgata ttggagaatg
    15181 acctgcagga tttcatcacg cacccaagcg gtggattgct cggcgccaat atcatctaaa
    15241 atcaggacct cagacagctt aatctcatcc accaaggtct taacattgcc atcactgata
    15301 gcatttttga catcaatgac aaagctagga tagtggagga gagttgatga aacaccacgt
    15361 ttttctgata aatcatgagc taaggccgcc accatgaaac ttttacccac accaaagtct
    15421 ccatataagt aaagaccttt tcgaatagct ggatattgct ccacgaaggc tagtagcttt
    15481 tcaaaaactg gtaagcgccc caaatcatcc aagtcaactt gagccaaact agctttcttg
    15541 agactggctg gtagattgat taacttgaga cggttcttaa tagccgcttc tttttcagcc
    15601 gcgattagct caggagtttc ttcatatgaa acatctgcat agccatgatt cttaaccaaa
    15661 atcggcttgt agcctttggc aatataatcc gtatccccac ggagaaactt gtcacgctcg
    15721 gtgatgtact gattaaactt ggagatactg cgatttaatt cctctggagt caaggattct
    15781 tgctggataa aagccgcaac atcagggtcc ttcatgattt tctggaccaa atcttgataa
    15841 taaaaacggc tgggttgacg tttgagtacg tctccgacac tttccatcta atctcctcct
    15901 ttttctaatc gagctaatag ttcttgcttc ttacgttcta gttccagacg agtttcctcg
    15961 ctggtttcat tcttatattc aggattactc catttaggaa cattggtttt tactggggca
    16021 gtctgattct gtttttgtgt ttttgctttc tgtcctcgct cacggatgcg caagactgcc
    16081 ttttctgccg aatgaatctt ttgataggca tagtcattgg ctaccttcat ggcatatttc
    16141 tcattgatat ttgccgaatc gaccttatta aaggtcaata aaagaatgat attgatgact
    16201 tcgtccagca agcccaagcc agccatctgt tgcaagagtt ctctttctgt ttgggtaatg
    16261 gttcccttgc gtgtttgctt gatttctgcc aagaactgca gggcagtttt acttttggct
    16321 tctttgataa tggttgtttc cttaagacta aagtcagagg aaactggttt ttgagcaatt
    16381 ttttcacgca tgcgtttggt tgaaataacc tgagaaacag ctgttgactt ggccaattga
    16441 taggtttcaa accaagtcca tttcttctcc tcggcaatag caaagaggtt taagacatcg
    16501 gactgctcat ccgcaaaacg aagtccatct cgagccatca gctggcgaaa atgttccaag
    16561 tcaaaatcat tggccacttt cttcttgaga ccaaggtctt cttgactgcc tagttctgcc
    16621 aattctggaa agacttgatt gagtgagaca ggtatttctt caccatcagc actttcaact
    16681 ttcaaatcct ccacagcaga gtcgccaatc tttttctcta agagtctgcg ataaacagga
    16741 tgctccaaga agtcttgact agatagagga gcatggaggg ctagctgata aacatccccc
    16801 ttttgataga gggtcaagag attaaaagca gataagattt tcaatgattt tatcagtcta
    16861 tccatcccaa agttgagatg attgaggata cttgaaaaaa gatattcctt tctaccatta
    16921 tcccaaaaac tgattgtata aagataaagg ctcagtgcct cctgaccgat aatcgggagg
    16981 tagcactgta ccagagatga ggtatcttgc gacacccgat tattctttag ataagaaaaa
    17041 cggtcaattg gcttcattta tctttccttt ttctttttag aggactgggt gatttgttgg
    17101 agcaagctct ctaactcact gacatcctta aaactacgat agacactagc aaaacgtaca
    17161 taggtaatct cgtccaattc agccaactcc tccatgacga gtgaaccaat gtcctcactt
    17221 tgaatttcat tttcatttcg accacggagt ttctgttcga tacgattgac taccatgttg
    17281 atttcatcac ttgacacagg acgtttctgg gctgagcgga taatcccatt aaagatttta
    17341 tctctggaga attgttcccg tgtaccatct tttttaacaa ccactaaggt tctttcttct
    17401 actcgttcgt aggttgtaaa acggtgttgg cattcgtcgc actcacgtct tctacgaatg
    17461 gtgttccctt cttctgcttg gcgactatcg ataacacttg acttggtagc cccacatttt
    17521 ggacaacgca tcctttccct ccttatcgtt ttcttttcat tataccattt tttaagcgat
    17581 tcccaaaaca attcttcttt ttgcttgaca agttttttgt tttgttgtac tatttaatta
    17641 agacaaaagg ataagagaaa ggagactaag atgtcctgga catttgacaa caaaaaaccc
    17701 atctatttac agattatgga gaaaatcaag cttcagattg tttcccatac actggaaccc
    17761 aatcaacaac ttccaaccgt gagggagcta gctagcgagg ctggtgtcaa tcccaatacc
    17821 atccaaagag ccttatcaga ccttgaacga gaaggatttg tctacagcaa gcgaacaact
    17881 ggacgatttg tgactaagga taaggagcta atcgcccagt cacgcaaaca attatcagaa
    17941 gaagaattgg aacacttcgt ttcctccatg acccattttg gctatgaaaa agaagaacta
    18001 ccaggcgtag tcagtgatta tattaaagga gtttaagcct atgtcattac tagcatttga
    18061 aaatgtatcc aaatcatatg gagcaacacc agcccttgaa aatgtttctc ttgacattcc
    18121 agctggaaaa attgtcggcc ttcttgggcc aaacggctca ggaaaaacaa ccctgattaa
    18181 actaattaat ggcctcttac aaccagatca aggacgtgtc ctcatcaacg acatagaccc
    18241 aagcccagca accaaggccg ttgtagctta tttgcctgat acgacctatc tcaatgagca
    18301 aatgaaggtc aaagaagccc ttacctactt caagaccttc tataaagatt tcaatcttga
    18361 acgcgctcat catctacttg cagacctggg cattgatgaa aatagtcgtc tcaagaaact
    18421 atcaaaagga aacaaagaaa aggttcaact gattttggtt atgagccgtg atgctcgtct
    18481 ctatgttttg gatgaaccca ttggtggggt ggatccagca gcccgtgctt atatcctcaa
    18541 taccattatc aacaactact caccaacttc taccgttttg atttctaccc acttgatttc
    18601 tgatatcgag ccaatcttgg atgaaattgt cttcctaaaa gacggaaaag tcgtccgtca
    18661 aggaaatgta gatgatattc gctacgagtc aggtgaatcc attgaccaac tcttccgtca
    18721 ggaatttaag gcctaagcaa aggagattat ttatgttttg gaatttagtt cgctacgaat
    18781 ttaaaaatgt taacaagtgg tatttagccc tctacgcagc cgtgctagtc ctttctgccc
    18841 tcatcggaat acagacacaa ggctttaaaa atctacctta ccaagaaagt caggctacta
    18901 tgctactttt tctagctaca gtctttggtg gcttgatgct tacacttggg atttcaacca
    18961 ttttcttgat tattaaacgc ttcaaaggta gtgtctacga ccgacaaggc tatctgactt
    19021 tgaccttgcc agtttctgaa caccatatca tcacagccaa actaatcggt gcctttatct
    19081 ggtcattgat tagcaccgct gtattggctc taagtgctgt tattattctg gctttaacag
    19141 ctccagaatg gattcctctt tcttatgtga ttacatttgt agaaacacat ctccctcaga
    19201 tctttcttac aggtatatcc ttcctactaa atactatttc aggaatcctc tgcatctacc
    19261 tggctatttc cattggacag cttttcaatg aataccgtac agcactcgct gttgcagtct
    19321 acattggtat ccaaatcgtc attggattta ttgaactttt cttcaatctt agttctaatt
    19381 tctatgtcaa ttcactggta ggactcaatg accatttcta tatgggagca ggtatagcca
    19441 ttgttgaaga actcatattc atagctatct tttatctcgg aacctactac atcttgagaa
    19501 ataaggttaa tttgctttaa ataattttta cctagatatg taacatactc atagaacaaa
    19561 agagaccagg caaaaagtct ttacaattag aaaacgcata gtatcaagtg ttaaaaatcc
    19621 ttgatattat gcgttttttg tgagctgact tatttccttt cactatatcg caaaatgaaa
    19681 taagaacgga acgatgggat tttggaattc aaatcaattt ataagaatgt tttagaagta
    19741 atattatcct attccagatt cagttcacta tacaattgag ttttcaagca acctgtttac
    19801 ataatgtgta cataattagg ttcgtgattc cacccttttc acctttaaaa acctcgcttt
    19861 cgcaaggctc ttctatttat aagataaggc acgtttaaag gttttccaaa tccctaaatc
    19921 atccgtttga agaacgagac tagcatacat gcgtccgata aatcctgttg ctaccaccgc
    19981 aaaaatcact gtaatagcaa gtgaaatcca tgcttctgct ccccccgcat agtcattaat
    20041 cgttcgaaac ggcataaaga aggtcgaaat aaagggaata taagaaccaa tcttcaagag
    20101 gagattgtca ccagctgcac ctagagctgt cactccaaaa aaaccaccca taatcaaaat
    20161 catcaaaggc gacaaggctt tccctgagtc ctcaggacga gaaaccatag atcctaggaa
    20221 ggctgccaag actacgtaca tgaaaagact gatcaaaata aagagcaagg tattcagtga
    20281 gatagcatct cccaagtgaa ccaaaatacc agactgagcc aagaatggca aatctttaaa
    20341 gagcaaaacg gcagccagac cacctacaac atagatccca atatgcgtta aaatcactag
    20401 aaacagagcc atcatccgcg catagaaata gtgacttgcc cttatgctag aaaaaacgac
    20461 ttccataatt ttggtgcctt tttcactggc aacttcctga gctgttacac ccgcataggt
    20521 aatcagaatc atataaagaa agaatcctaa ggcacctgct gcaattgttt gaataaactt
    20581 tttattttcc ttgacttcat caatcttttc tgtgaattga attgtctgcg ctaagcgttt
    20641 ttcctgctct tgagacaagg aagcagttga acgattaagc tgattttgca gttcattgag
    20701 tatacctgta acctcaaatt taattccatt ttcaagcgat gtttcgccat gataaactgc
    20761 ctttagaaca ctatcttctt gatcaatggt caaataacct tttaattttt cttctttaat
    20821 tgcttctttg gcacttgctt cgtctttata gtcgaagtta acaccattta cattcttcag
    20881 tccttctgct acagatggca ctgttgtcac tactgccact ttattatttt tagccataga
    20941 agaaccttgg agatgcccaa ttcctacaga gattcctaaa aagaggaacg gcgaaatcac
    21001 cataaagaag aaactccatg actcaacatg tcgaagatag gtttccttga ttacaaccca
    21061 catatttctc atacttccac tcctgattct agtttaaaga tttcatcgat agttggcgct
    21121 tgttggtcaa atgttgcgat atattgacct tgagtcaaga ttgggaagag ttcccttcca
    21181 gcgctctcat cctccaaaat caatttccaa ctgccttgtt tggtcaagct cacctgtttg
    21241 acatgaggaa gattttccaa ttcttccttg cttcgttcac ttgaaacaaa gagacgcgtt
    21301 ttcccgtatt gattgcggac atcctgaact ggtccgtgca agaccacacg gccatctcgg
    21361 atcatcagaa tatcgtcaca aagttcctca acattggtca tgacatggtc agaaaagata
    21421 atggttgctc cgcgctcttt ttcctgaaaa atgacttgtt tgagcaattc tgtattaact
    21481 gggtccaatc cactaaaagg ctcatccaag ataatcaggt ctggttcatg aatcagagta
    21541 ataatgagct gaatcttctg ctgatttcct tttgacagac tcttgatttt atctgtcagc
    21601 tttcctttca cttccaacct cttcatccat tgagggagtt tttctttgac ttctttggca
    21661 tccatgcctt ttagagtcgc caagtagcga acttgttcaa gaactgtcaa tttaggcatg
    21721 agactgcgtt cttcaggcag ataaccaatc cgagcatagg tctcctgacg aatatcctga
    21781 ccatccagac cgatttctcc ctgatattct aggaatttta aaatactatg gaaaatcgtt
    21841 gtttttccag caccattttt tccgactagt cccaaaatac gacctggtcg cgcttgaaag
    21901 tcaataccaa acaaaacttg cttggatcca aaacttttct ctagacttct tacttctagc
    21961 atctttcacc tccgaaattt cttgcactca ttatactcct ttttgatagc ctttacaatg
    22021 tttttgtcca tttttagaag actattgctg tgtaaaatat ggcctggagc acttttatac
    22081 tcaatgaaaa gcaaagagca aactaggaag ctagccgtag gctgctcaaa gtacagcttt
    22141 gaggttgcag ataaaactga cgaagtcagc tcaaaacact gttttgaggt tgtggataga
    22201 actgacgaag tcagtaacta tatctacggc aaggcgaagc tgacgtggtt tgaagagatt
    22261 ttcgaagagt attagtgata aatccattat acagtagcaa acttaattta taccttccgc
    22321 tcctcaactg tctattttta atcctgaatt gttatttgag taactccttt ttcctcataa
    22381 agttttcttc ctctaaaact tctggaaaaa ggcgaatagt ttcagacaac atttttataa
    22441 gaaacaagtt catctgtcat ttcaagaagg agtaatcctt tatctactaa tggacggaac
    22501 agaattcaac cgcttgtccc atatgttttc taaagattat atagtaaaat gaaataaaaa
    22561 caggacaaat tgatcaggac agtcaaattg atttctaaca atgttt
//
LOCUS       DAVLVP010000022        22078 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.022, whole genome shotgun sequence.
ACCESSION   DAVLVP010000022 DAVLVP010000000
VERSION     DAVLVP010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 22078)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22078)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22078
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.022"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            86..2308
                     /gene="recJ"
                     /locus_tag="VYN69_000846"
     CDS             86..2308
                     /gene="recJ"
                     /locus_tag="VYN69_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEV1533146.1"
                     /translation="MITPTYEWQFALQVEDADFTKIAKKAGLGPEVARLLFERGIQNQ
                     ESLKKFLEPSLEDLHDAYLLHDMDKAVERIRQAIEEGENILVYGDYDADGMTSASIVK
                     ESLEQLGAECRVYLPNRFTDGYGPNASVYKYFIEQEGISLIVTVDNGVAGHEAIALAQ
                     SMGVDVIVTDHHSMPETLPDAYAIVHPEHPDADYPFKYLAGCGVAFKLACALLEEVQV
                     ELLDLVAIGTIADMVSLTDENRILVQYGLEMLGHTQRIGLQEMLDMAGIAANEVTEET
                     VGFQIAPRLNALGRLDDPNPAIDLLTGFDDEEAHEIALMIHQKNEERKEIVQSIYEEA
                     KTMVDPEKKVQVLAKEGWNPGVLGIVAGRLLEELGQTVIVLNIEDGRAKGSARSVEAV
                     DIFEALDPHRDLFIAFGGHAGAAGMTLEVEQLSDLSQVLEDYVREKGADAGGKNKLNL
                     DEELDLEALSLETVKSFERLAPFGMDNQKPIFYIKNFQVESARTMGAGNAHLKLKISK
                     GEASFEVVAFGQGRWATEFSQTKNLELAVKLSVNQWNGQTALQLMMVDARVEGVQLFN
                     IRGKNAVLPEGVPVLDFPGELPNLAASEAVVVKNIPEDITQLKTIFQEQHFSAVYFKN
                     DIDKAYYLTGYGTRDQFAKLYKTIYQFPEFDIRYKLKDLAAYLNIQQILLVKMIQVFE
                     ELGFVTIKDGVMTVNKEAPKREIGESQIYQNLKQTVKDQEMMALGTVQEIYDFLMEKE
                     "
     gene            2483..4144
                     /locus_tag="VYN69_000847"
     CDS             2483..4144
                     /locus_tag="VYN69_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1533147.1"
                     /translation="MSNISLTTLGGVRENGKNMYIAEIGESIFVLNVGLKYPENEQLG
                     VDVVIPNMDYLFENSDRIAGVFLTHGHADAIGALPYLLAEAKVPVFGSELTIELAKLF
                     VKGNDAVKKFNDFHVIDENTEIDFGGTVVSFFPTTYSVPESLGIVLKTSEGSIVYTGD
                     FKFDQTASESYATDFARLAEIGRDGVLALLSDSANADSNIQVASESEVRDEITQTIAD
                     WEGRIIVAAVSSNLSRIQQIFDAADKTGRRIVLTGFDIENIVRTAIRLKKLSLANEIL
                     LIKPKDMSRFEDHELIILETGRMGEPINGLRKMSIGRHRYVEIKDGDLVYIATAPSIA
                     KEAFVARVENMIYQAGGVVKLITQSLHVSGHGNVRDLQLMINLLQPKYLFPVQGEYRE
                     LDAHAKAAMAVGMLPERIFIPKKGTTMAYENGDFVPAGSVSAGDILIDGNAIGDVGNV
                     VLRDRKVLSEDGIFIVAITVNRREKKIVARARVHTRGFVYLKKSRDILRESSELINQT
                     VEEYLQGDDFDWADLKGKVRDNLTKYLFDQTKRRPAILPVVMEAK"
     gene            4213..4992
                     /locus_tag="VYN69_000848"
     CDS             4213..4992
                     /locus_tag="VYN69_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEV1533148.1"
                     /translation="MAVMKIEYYSQVLDMEWGVNVLYPDANRVEEPECEDIPVLYLLH
                     GMSGNHNSWLKRTNVERLLRGTNLIVVMPNTSNGWYTDTQYGFDYYTALAEELPQVLK
                     RFFPNMTSKREKTFIAGLSMGGYGCFKLALATNRFSHAASFSGALSFQNFSPESQNLG
                     SPAYWRGVFGEIRDWTTSPYSLESLAKKSDKKTKLWAWCGEQDFLYEANNLAVKNLKK
                     LGFDVTYSHSAGTHEWYYWEKQLEVFLTTLPIDFKLEERLT"
     gene            5104..6324
                     /gene="murM"
                     /locus_tag="VYN69_000849"
     CDS             5104..6324
                     /gene="murM"
                     /locus_tag="VYN69_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001819885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurM"
                     /protein_id="HEV1533149.1"
                     /translation="MYRYQIGIPTLEYDQFVKEHELANVLQSSAWEEVKSNWQHEKFG
                     VYREEKLLATASILIRTLPLGYKMFYIPRGPILDYGDKELLNFAIQSIKSYARSKRAV
                     FVTFDPSICLSQSLINQEKTEFPENLAIIDSLQQMGVRWSGKTEEMGDTIQPRIQAKI
                     YKENFEEDKLSKSTKQAIRTARNKGLEIQYGGLELLDSFSELMKKTEKRKEIHLRNEA
                     YYKKLLDNFKDKAYITLATLDVSKRSQELEEQLAKNRALEETFTESTRTSKVEAQKKE
                     KERLLEELTFLQEYIDVGQARVPLAATLSLEFGTTSVNIYAGMDDDFKRYNAPILTWY
                     ETARYAFERGMVWQNLGGVENSLNGGLYHFKEKFNPTIEEYLGEFTMPTHPLYPLLRL
                     ALDFRKTLRKKHRK"
     gene            6329..7561
                     /gene="murN"
                     /locus_tag="VYN69_000850"
     CDS             6329..7561
                     /gene="murN"
                     /locus_tag="VYN69_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation alanyltransferase
                     MurN"
                     /protein_id="HEV1533150.1"
                     /translation="MALTTLTKEEFQTYSDQVSSRSFMQSVQMGDLLEKRGARIVYLA
                     LKQEGEIQVAALVYSLPMLGGLHMELNSGPIYTQQDALPVFYAELKEYAKQNGVLELL
                     VKPYETYQTFDSQGNPIDAEKKSIIQDLTDLGYQFDGLTTGYPGGEPDWLYYKDLTEL
                     TEKSLLKSFSKKGKPLVKKAETFGIRLKKLKREELSIFKNITKETSERREYSDKSLEY
                     YEHFYDTFGEQAEFLIASLNFSDYMSKLQGEQSKLEENLDKLRLDLSKNPHSEKKQNQ
                     LREYSSQFETFEVRKAEARDLIEKYGEEDIVLAGSLFVYMPQETTYLFSGSYTEFNKF
                     YAPALLQKYVMLESIKRGIPKYNFLGIQGIFDGSDGVLRFKQNFNGYIVRKAGTFRYH
                     PSPLKYKAIQLLKKIVGR"
     gene            7650..8351
                     /locus_tag="VYN69_000851"
     CDS             7650..8351
                     /locus_tag="VYN69_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001267193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="matrixin family metalloprotease"
                     /protein_id="HEV1533151.1"
                     /translation="MRWLFRLIGAFFSFVWRLFWRLVWIVVLLCVLAFGLLWYLNGDF
                     QGALKQAERSVKIGQQSIDQWEKTGQLPKLSQTDSHQHSEGRWPQASARIYLDPQMDS
                     RFQEAYLEAIQNWNQTGAFNFELVTESSKADITATEMNDGGTPVAGEAESQTNLLTGQ
                     FLSVTVRLNHYYLSNPYYGYSYERLVHTAEHELGHAIGLDHTDEKSVMQPAGSFYGIQ
                     EEDVANLRKIYETSE"
     gene            8391..10235
                     /gene="uvrC"
                     /locus_tag="VYN69_000852"
     CDS             8391..10235
                     /gene="uvrC"
                     /locus_tag="VYN69_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEV1533152.1"
                     /translation="MNNLIKSKLELLPTSPGCYIHKDKNGTIIYVGKAKNLRNRVRSY
                     FRGSHNTKTEALVSEIVDFEFIVTESNIEALLLEINLIKENKPKYNIMLKDDKSYPFI
                     KITNERYPRLIITRQVKKDGGLYFGPYPDVGAANEIKRLLDRIFPFRKCTNPPSKVCF
                     YYHIGQCMAHTICKKDEAYFKSMAQEVSDFLKGQDDKIIDDLKSKMAVAAQSMEFERA
                     AEYRDLIQAIGTLRTKQRVMAKDLQNRDVFGYYVDKGWMCVQVFFVRQGKLIERDVNL
                     FPYFNDPDEDFLTYVGQFYQEKSHLVPNEVLIPQDIDEEAVKALVDTKILKPQRGEKK
                     QLVNLAIKNARVSLEQKFNLLEKSVEKTQGAIENLGRLLQIPTPVRIESFDNSNIMGT
                     SPVSAMVVFVNGKPSKKDYRKYKIKTVVGPDDYASMREVIRRRYGRVQREALTPPDLI
                     VIDGGQGQVNIAKQVIQEELGLDIPIAGLQKNDKHQTHELLFGDPFEVVDLSRNSQEF
                     FLLQRIQDEVHRFAITFHRQLRSKNSFSSQLDGIDGLGPKRKQNLMKHFKSLTKIKEA
                     SVDEIVEVGVPRVVAEAVQRKLNPQGEALPQVAEERVDYQTEGNHNEP"
     gene            10225..11058
                     /locus_tag="VYN69_000853"
     CDS             10225..11058
                     /locus_tag="VYN69_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001015777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1533153.1"
                     /translation="MNHKIAILSDVHGNATALEAVIADAKNQGASEYWLLGDIFLPGP
                     GANDLVALLKDLPITASVRGNWDDRVLEALDGQYGLEDPQEVQLLRMTQFLMERMDPA
                     TIVWLRSLPLLEKKEIDGLRFSISHNLPDKNYGGDLLVENDTEKFDQLLDAETDVAVY
                     GHVHKQLLRYGSQGQQIINPGSIGMPYFNWEALKNHRAQYAVIEVEDGELLNIQFRKV
                     AYDYEAELELAKSKGLPFIEMYEELRREDNYQGHNLELLASLIEKHGYVEDVKDFFDF
                     L"
     gene            complement(11240..12040)
                     /locus_tag="VYN69_000854"
     CDS             complement(11240..12040)
                     /locus_tag="VYN69_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1533154.1"
                     /translation="MKKFSLLLAILPFLVACGNQATPKETSAQKTIVLATAGDVPPFD
                     YEDKGNLTGFDIEVLKAVDEKLSDYEIQFQRTAWESIFPGLDSGHYQAAANNLSYTKE
                     RAEKYLYSLPISNNPLVLVSNKKNPLTSLDQIAGKTTQEDTGTSNAQFINNWNQKHTD
                     NPATINFSGEDIGKRILDLANGEFDFLVFDKVSVQKIIKDRGLDLSVVDLPSADSPSN
                     YIVFSSDQKEFKEQFDKALKELYQDGTLEKLSNTYLGGSYLPDQSQLQ"
     gene            12211..12816
                     /locus_tag="VYN69_000855"
     CDS             12211..12816
                     /locus_tag="VYN69_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002924506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEV1533155.1"
                     /translation="MKFLELNKKRHATKHFTDKPVDPKDVRTAIEIATLAPSAHNSQP
                     WKFVVVREKNAELAKLAYGSNFEQVSSAPVTIALFTDTDLAKRARKIARVGGANNFSE
                     EQLQYFMKNLPAEFARYSEQQVSDYLALNAGLVAMNLVLALTDQGIGSNIILGFDKSK
                     VNEVLEIEDRFRPELLITVGYTDEKLEPSYRLPVDEIIEKR"
     gene            12829..14229
                     /gene="pepV"
                     /locus_tag="VYN69_000856"
     CDS             12829..14229
                     /gene="pepV"
                     /locus_tag="VYN69_000856"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEV1533156.1"
                     /translation="MTAIDFTAEVEKRKEDLLADLFSLLEINSERDDSKADAQHPFGP
                     GPVKALEKFLEIADRDGYPTKNVDNYAGHFEFGDGEEVLGIFAHMDVVPAGSGWDTDP
                     YTPTIKDGRLYARGASDDKGPTTACYYGLKIIKELGLPTSKKVRFIVGTDEESGWADM
                     DYYFEHVGLAKPDFGFSPDAEFPIINGEKGNITEYLHFAGENTGVARLHSFTGGLREN
                     MVPESATAVVSGDLADLQAKLDAFVAEHKLRGELQEEAGKYKVTIIGKSAHGAMPASG
                     VNGATYLALFLSQFGFAGPAKDYLDIAGKILLNDHEGENLKIAHVDEKMGALSMNAGV
                     FRFDETSADNTIALNIRYPKGTSPEQIKSILENLPVVSVSLSEHGHTPHYVPMEDPLV
                     QTLLNIYEKQTGLKGHEQVIGGGTFGRLLERGVAYGAMFPDSIDTMHQANEFIALDDL
                     FRAAAIYAEAIYELIK"
     gene            14283..14861
                     /locus_tag="VYN69_000857"
     CDS             14283..14861
                     /locus_tag="VYN69_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HEV1533157.1"
                     /translation="MSQIERIKQAIMADSQNASYTERGIEPLFAAPKTARINIIGQAP
                     GLKTQEAGLYWKDKSGDRLRDWLGVDEDTFYNSGYFAVLPMDFYFPGHGKSGDLPPRT
                     GFAEKWHPQLLQELPDIQLTLLIGQYAQAYYLQEKISGKVTERVKHYKDYLPAYFPLV
                     HPSPRNQIWMAKNPWFEAEVVPDLKKRIKTIL"
     gene            15021..15173
                     /locus_tag="VYN69_000858"
     CDS             15021..15173
                     /locus_tag="VYN69_000858"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1533158.1"
                     /translation="MKEIAFDAFYQLYQNDQLSLVDVREVDEFAALHLEGAHNLPLSQ
                     LTDSYD"
     gene            15216..15308
                     /locus_tag="VYN69_000859"
     CDS             15216..15308
                     /locus_tag="VYN69_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1533159.1"
                     /translation="MRSARACQFLLEQGYNVINVQGGMLAFEEL"
     gene            15479..16804
                     /gene="brnQ"
                     /locus_tag="VYN69_000860"
     CDS             15479..16804
                     /gene="brnQ"
                     /locus_tag="VYN69_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006152071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEV1533160.1"
                     /translation="MAKKGTLTGLLLFGIFFGAGNLIFPPSLGALSGEHFLPAIAGFV
                     FSGVGIAVLTLIIGTLNPKGYIYEISTKIAPWFATLYLSVLYLSIGPFFATPRTATTA
                     YEVGISPLLSDANKGLGLIVFTVLYFAAAYLISLNPSKILDRIGRILTPVFAILIVIL
                     VVLGAIKYGGTSPQAASAAYQASAFGTGFLEGYNTLDALASVAFSVIAVQTLKQLGFS
                     SKKEYISTIWVVGIVVALAFSALYIGLGFLGNHFPVPAEAMKGGTPGVYILSQATQEI
                     FGSTAQLFLAAMVTVTCFTTTVGLIVSTAEFFNERFPQISYKVYATAFTLIGFAIANL
                     GLDAIIKYSIPVLVILYPITIAIVMIVIVNKFVALSKPGMQLTIAVVTVIAIASVLGS
                     SFKVEFLANLVSVLPFAKASLPWLVPAIVGILLSLVLPNKQESDVFEME"
     gene            17008..18045
                     /locus_tag="VYN69_000861"
     CDS             17008..18045
                     /locus_tag="VYN69_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001077216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEV1533161.1"
                     /translation="MNQTVEYIKELTAIASPTGFTREIADYLVKTLEGFGYQPVRTSK
                     GGVNVTIKGQNDEQHRYVTAHVDTLGAIVRAVKPDGRLKMDRIGGFPWNMIEGENCTV
                     HVASTGQKVSGTILIHQTSCHVYKDAGTAERTQDNMEVRLDAKVSNEKETRALGIEVG
                     DFISFDPRTVVTETGFIKSRHLDDKVSAAILLNLLRIYKKEKIELPVTTHFAFSVFEE
                     VGHGANSNIPAQVVEYLAVDMGAMGDGQQTDEYTVSICVKDASGPYHYDFRQHLVTLA
                     KEQDIPFKLDIYPFYGSDASAAMSAGAEVKHALLGAGIESSHSYERTHIDSVVATERM
                     VDAYLKSEAVS"
     gene            18047..18508
                     /locus_tag="VYN69_000862"
     CDS             18047..18508
                     /locus_tag="VYN69_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1533162.1"
                     /translation="MCLICQRIELIKAGQNPYFVKELETGYVVIGDYQYFKGYTLFLA
                     KDHVTELHHMETSVKLRFLEEMSLVQEAVAKAFEAEKMNIELLGNGDAHAHWHLFPRR
                     AGDMKSHGLNGRGPVWWVPWEEMAAEDCQVQSPELEEMIKILSHELEKYLA"
     gene            18526..19242
                     /locus_tag="VYN69_000863"
     CDS             18526..19242
                     /locus_tag="VYN69_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEV1533163.1"
                     /translation="MKKRYVILSGLLALTLAACSQEKTKVEENTQKTEQSSQPEGTVG
                     SKSQASSQKKAEVSNKGSYYSIQGKYDEIILANKRYPLSKDYNPGENPTAKAELLKLI
                     AAMQAEGYPISDQYSGFRSYETQAKLYQDYVNQDGKEAADRYSARPGYSEHQTGLAFD
                     LIGTDGDLVTEEKAAQWLLDHAADYGFVVRYLKGKEKETGYMAEEWHLRYVGKEAKEI
                     AASGLSLEEYYGFEGGDYVD"
     gene            19365..19790
                     /gene="rplK"
                     /locus_tag="VYN69_000864"
     CDS             19365..19790
                     /gene="rplK"
                     /locus_tag="VYN69_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004226069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEV1533164.1"
                     /translation="MAKKVEKLVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGMIIPVVISVYEDKSFTFVTKTPPAAVLLKKAAGVEKGSGTPNKTKVATVTR
                     AQVQEIAETKMPDLNAANVESAMRMIEGTARSMGFTVVD"
     gene            19998..20687
                     /gene="rplA"
                     /locus_tag="VYN69_000865"
     CDS             19998..20687
                     /gene="rplA"
                     /locus_tag="VYN69_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEV1533165.1"
                     /translation="MAKKSKQLRAALEKIDSTKAYSVEEAVALAKETNFAKFDATVEV
                     AYNLNIDVKKADQQIRGAMVLPNGTGKTSRVLVFARGAKAEEAKAAGADFVGEDDLVA
                     KINDGWLDFDVVIATPDMMALVGRLGRVLGPRNLMPNPKTGTVTMDVAKAVEESKGGK
                     ITYRADRAGNVQAIIGKVSFEAEKLVENFKAFNETIQKAKPATAKGTYVTNLTITTTQ
                     GVGIKVDVNSL"
     gene            21065..21223
                     /locus_tag="VYN69_000866"
                     /pseudo
     CDS             21065..21223
                     /locus_tag="VYN69_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(21333..21479)
                     /locus_tag="VYN69_000867"
     CDS             complement(21333..21479)
                     /locus_tag="VYN69_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533166.1"
                     /translation="MKTKEQTRKLASGCSKHCFEVVDRTDVVSNHTHGKATLTWFEEI
                     FEEY"
     gene            21493..21855
                     /locus_tag="VYN69_000868"
     CDS             21493..21855
                     /locus_tag="VYN69_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HEV1533167.1"
                     /translation="MLEVGLNSLISLFTFTFDILYPIVKVGHTDDYSHGAVKLSVSLV
                     DKDVMKKISVTVIGYFEINIDENITDILYVNGTAILYHYLRSIVSIVSAIDSSEAMLL
                     PTINVLELLDKSQPFEEE"
BASE COUNT         6598 a         4050 c         5111 g         6319 t
ORIGIN      
        1 ttataaaaaa gcaatctgga tagagaaaac gtctaaatca tgttataatg aagcaataga
       61 attcttagaa agagtggatg tctttttgat aacacctact tatgaatggc agtttgccct
      121 gcaggtagaa gatgcggatt ttacaaagat agccaagaag gctggactgg gtcctgaggt
      181 ggctcggtta ttgtttgaga gagggattca gaaccaagaa agtctgaaga agtttttaga
      241 accttccttg gaggacttac atgatgctta tctgctccat gatatggaca aggcagtgga
      301 gcggattcgt caggctattg aagaagggga aaatattctt gtttatggag actatgatgc
      361 ggatggcatg acttcggctt ctattgtgaa ggaaagtttg gaacaacttg gtgctgagtg
      421 ccgagtttac ctgccaaatc gttttaccga tggctatggc cctaatgcta gtgtttataa
      481 atactttatc gagcaagaag ggatttcctt gattgtgacg gtggacaatg gggttgctgg
      541 tcatgaggct attgcattgg ctcagtctat gggagtagat gtcattgtga cagaccatca
      601 ttccatgcct gaaaccctgc cagatgctta tgctattgtc catcctgaac atccagatgc
      661 ggattatcct tttaaatatt tggctggttg tggagttgct ttcaagttgg cttgtgccct
      721 gttagaagaa gtgcaagtgg aattgcttga tttggtcgct attggaacta ttgcagatat
      781 ggtgagtctg acggatgaaa atcgtatctt agttcaatat ggtctggaaa tgttgggtca
      841 tacccagcgc attggtctgc aagaaatgct ggacatggct gggattgctg ccaacgaagt
      901 aacagaagaa acggttggtt tccagattgc tcctcgtttg aatgccttgg gtcgcttgga
      961 tgatcccaat cctgccattg atttgttgac tggatttgat gatgaggaag cgcatgagat
     1021 tgcccttatg attcaccaga aaaacgaaga gcgcaaggaa atcgttcagt ctatctatga
     1081 agaagccaag accatggtgg atcctgagaa gaaggttcag gtcttggcca aggaaggctg
     1141 gaatcctggg gttctaggaa tcgtggctgg tcgtttattg gaagaattgg gacagacagt
     1201 cattgttctt aatatagaag acggtcgtgc caagggcagt gctcgtagtg tggaagcggt
     1261 cgatattttt gaagctctgg atccccatcg agacctcttc atcgcctttg gaggtcatgc
     1321 aggtgcagcg ggtatgacgc tggaagttga gcaactctca gatttatctc aggttttgga
     1381 agattatgtt cgtgaaaaag gtgcagatgc tggtggcaag aataagttaa acctagatga
     1441 agagttggat ttggaggcac ttagcttgga aacggtcaaa agttttgaac gtttagctcc
     1501 ttttggaatg gataatcaga aacctatttt ttatatcaag aattttcagg tcgaaagtgc
     1561 tcgtactatg ggggcaggta atgcccatct aaagctgaaa atttccaagg gtgaggcgag
     1621 ttttgaagtg gtagcctttg gtcaaggcag atgggcgaca gagttttctc aaaccaagaa
     1681 tctagagtta gcggttaaat tgtctgtcaa ccaatggaat ggccaaactg ccctccagtt
     1741 gatgatggtg gatgcgcgag tggaaggtgt tcaacttttt aacattcgtg gaaaaaatgc
     1801 agtcttgcca gaaggtgttc cagtcttgga ttttcctgga gaactgccaa atcttgcggc
     1861 tagtgaagct gttgtcgtaa aaaacattcc agaggatatt actcagctga agaccatttt
     1921 tcaggaacag catttctctg ctgtctattt caaaaatgat attgacaagg cttattatct
     1981 gacaggttat gggactagag atcagtttgc caaattgtac aagactattt accagttccc
     2041 agagtttgat attcgctaca agctgaaaga tttggctgca tatcttaata ttcaacaaat
     2101 cttgctggtc aagatgattc aagtatttga agaactaggc tttgtgacga taaaagatgg
     2161 tgtgatgaca gtcaataaag aggcgccaaa gcgggagata ggagaaagtc aaatttacca
     2221 aaatctcaaa caaaccgtta aagaccaaga aatgatggcg ctgggtacgg tgcaagaaat
     2281 ttatgatttt ttgatggaaa aagagtagaa gttaggaaag agttgggaaa tcaactcttt
     2341 tttgaaaaca gaccttcatt ttgaaaatca tcaaaaaaat ggtataatgg taggaaaaga
     2401 ttcggctgaa agtattagaa cttttagaat aagagggtag agttgcccta taatcaagat
     2461 aaactaagat tttggaggaa aaatgagtaa tatcagttta acaacacttg gtggtgtgcg
     2521 tgagaatgga aaaaatatgt acattgctga aattggagag tccatttttg ttttgaatgt
     2581 agggttaaaa tatcctgaaa atgaacaatt aggggtcgat gtggtgattc caaacatgga
     2641 ttaccttttt gaaaatagcg accgtattgc tggggttttc ttgacccacg ggcatgcgga
     2701 tgccattggt gctctacctt atctcttggc agaggctaaa gttcctgtat ttgggtctga
     2761 gttgaccatt gagttggcaa agctctttgt caaaggaaat gatgccgtta agaaatttaa
     2821 tgatttccat gtcattgatg agaatacgga gattgatttt ggtgggacag tggtttcctt
     2881 cttccctacg acttactccg ttccagagag tctgggaatt gtcttgaaga catcggaagg
     2941 aagcatcgtt tatacaggtg acttcaaatt tgaccaaacg gctagtgaat cttatgcaac
     3001 tgattttgct cgtttggcag agattggtcg tgacggcgtc ctggctctcc tcagtgattc
     3061 ggccaatgca gacagcaata ttcaggtggc tagtgaaagt gaagttaggg atgaaattac
     3121 ccaaactatt gctgactggg aaggtcgtat catcgttgca gctgtttcca gtaatctttc
     3181 tcgtattcag cagatttttg acgctgcgga taaaacaggt cgacgtatcg tcttgacagg
     3241 atttgatatt gaaaatatcg tccgcacagc gattcgtctt aagaagttgt ctttagccaa
     3301 cgaaattctc ttgattaagc ctaaagatat gtctcgcttt gaagaccatg agttgattat
     3361 tcttgagaca ggtcgtatgg gtgaacctat caatggactt cgtaagatgt cgattggtcg
     3421 ccatcgttat gtagaaatca aggatgggga cctggtctat attgctacgg ctccgtctat
     3481 tgctaaagaa gcctttgttg cgcgtgtgga aaatatgatt tatcaggcag gtggggttgt
     3541 caaattgatt acccaaagtt tacatgtatc agggcacgga aatgtgcgtg atttgcagct
     3601 gatgatcaat cttttgcaac ctaagtacct cttccctgtc caaggggagt atcgtgagtt
     3661 ggatgctcac gctaaggctg ccatggcagt tgggatgttg ccagaacgca tcttcattcc
     3721 taaaaagggg acgaccatgg cttacgagaa tggagacttt gttccagctg gatcggtttc
     3781 agcaggagat atcttgattg atgggaatgc cattggtgat gttggaaatg ttgttcttcg
     3841 tgaccgtaag gtcttgtcag aggatggaat tttcatcgtg gctattacag tcaaccgtcg
     3901 tgagaagaaa attgtggcta gggctcgtgt tcacacgcgt ggatttgttt atctcaagaa
     3961 gagtcgcgat attctccgtg aaagttcaga attgattaac caaacggtag aagagtatct
     4021 tcaaggagat gactttgact gggcagatct taaagggaag gttcgagata atttgaccaa
     4081 gtatctcttt gaccaaacca agcgtcgtcc agctatttta ccagtagtca tggaagcaaa
     4141 ataatcgttg aaataaacag agagaaagtc gagtttcggc tttttcttat agaaaaatag
     4201 aaggagaaaa tcatggcagt aatgaaaatc gagtattact cacaagtatt ggatatggag
     4261 tggggggtga atgtcctcta ccctgatgcc aatcgagtgg aagaaccaga gtgtgaagat
     4321 attcccgtct tgtacctttt gcacgggatg tctggaaatc ataatagttg gcttaagcgg
     4381 accaatgtag aacgcttgct tcgaggaact aatctcatcg ttgttatgcc caataccagc
     4441 aatggttggt acaccgatac ccagtatggt tttgactact acacggctct agcagaggaa
     4501 ttgccacagg ttctgaaacg cttcttccct aatatgacta gcaagcgtga aaagaccttt
     4561 atcgctggtc tttctatggg aggttacggc tgcttcaaac tggctcttgc gacaaatcgt
     4621 ttttctcatg cagctagttt ttcaggtgcc ctcagctttc aaaacttttc tcctgaaagt
     4681 caaaatctgg gaagtccagc ctactggaga ggtgtttttg gagagattag agactggaca
     4741 actagtccct attctcttga aagtctggct aaaaaatcgg ataaaaagac caaactttgg
     4801 gcgtggtgtg gcgaacagga tttcttgtac gaagccaata atctcgcagt gaaaaatctc
     4861 aaaaaactag gttttgatgt gacctatagc catagtgctg gaactcacga gtggtactac
     4921 tgggaaaaac aattggaagt ttttttaaca accctaccaa ttgatttcaa attagaagag
     4981 agactgactt agtttgaact tcagcatagg gggagtagaa ctaaaataaa atatgttttc
     5041 actagacttt tcaaacgaaa gtagtagaat agtaataaaa tactggagga aagagagtag
     5101 gaaatgtacc gttatcaaat tggcattccc acattagaat atgatcagtt tgtcaaagaa
     5161 catgaattag ccaatgtatt acaaagtagt gcttgggagg aagttaagtc taattggcaa
     5221 catgagaagt ttggtgttta cagggaagaa aaattactgg cgacagctag tattttgatt
     5281 agaactcttc cgctaggcta taaaatgttt tacatcccaa gaggacctat attggattat
     5341 ggggataaag aactcttgaa ttttgccatt cagtctatta agtcctatgc tcgcagtaag
     5401 agagcggttt ttgtgacttt tgacccaagt atttgcctat ctcaaagttt aatcaatcag
     5461 gaaaagacag aatttcctga aaatctggct attattgata gtttgcaaca aatgggagta
     5521 aggtggtcag gaaaaacgga ggaaatggga gacaccattc aacctcgtat tcaggcgaaa
     5581 atatacaagg agaattttga agaagataaa ctttccaagt caacaaaaca ggctattcga
     5641 acagcacgaa acaaagggct tgagattcaa tatggtggac tggaactatt agattcattt
     5701 tcggagttga tgaaaaaaac tgagaagcga aaagagattc atttgaggaa tgaagcctat
     5761 tataaaaaat tgttagataa ttttaaggac aaggcctata tcaccttggc caccttggat
     5821 gtttctaaac gttcgcaaga attagaagaa cagttagcga aaaatagagc cttggaagag
     5881 acgtttactg agtcgactcg aacttcaaaa gtagaagcgc agaagaagga aaaagaacgt
     5941 ttgttagagg aattgacctt cttgcaggaa tatatagatg taggtcaagc gagagttcct
     6001 ttagcggcta ctttgagttt ggaatttggt actacctctg tcaatatata tgctggtatg
     6061 gatgatgatt ttaaacgtta caatgcacca attttaacat ggtatgaaac ggctcgctat
     6121 gcctttgagc gaggtatggt ctggcaaaat ttaggtggtg ttgaaaactc tctcaatggt
     6181 ggactttatc attttaagga aaaatttaat ccaacgattg aagaatactt gggtgaattt
     6241 acaatgccca ctcatcctct ctatcctctg ttaagacttg ctcttgattt ccgtaaaaca
     6301 ttaagaaaaa aacatagaaa gtaagtatat ggcactaaca acactcacga aagaagagtt
     6361 tcagacttat tctgatcagg tttcttctcg ttcctttatg caatctgtcc agatggggga
     6421 tttgctagaa aaaagagggg ctcgaattgt ttatcttgct ttgaaacaag aaggagaaat
     6481 tcaagttgca gctctggttt atagcctgcc catgctgggt ggtctgcata tggaactcaa
     6541 ttcggggccg atttataccc aacaagatgc tcttccagtt ttttatgcag agttaaaaga
     6601 atatgccaag caaaatggtg tattagagtt gcttgtaaaa ccctatgaaa cttatcaaac
     6661 ttttgatagc caaggtaatc caatagatgc tgagaaaaaa agtattattc aagatttgac
     6721 tgatttaggt tatcaatttg atggcttaac aacaggttac ccaggtggag aaccagattg
     6781 gttatactat aaagatttaa ctgaattaac tgaaaagagt ttgcttaaaa gttttagcaa
     6841 aaagggtaaa cccttggtga aaaaggctga aacctttggc attcggttga aaaagttaaa
     6901 acgtgaagaa ctatcgattt ttaagaatat aacaaaagaa acctctgaac gtagagaata
     6961 tagtgataaa agtttagaat attatgagca tttttatgat acttttggag aacaagcgga
     7021 gtttctcata gcaagcttaa atttttcgga ctatatgagc aaattgcaag gtgaacaaag
     7081 taaactagaa gaaaacttgg acaagttgcg acttgatttg agtaaaaatc ctcattctga
     7141 gaaaaaacaa aatcaactga gagaatattc tagtcaattt gaaacgtttg aagttcgaaa
     7201 agcagaagcg cgagacttga ttgaaaaata tggagaagaa gatattgttt tagctggtag
     7261 tttatttgtt tatatgcctc aggaaacgac ttatctcttt agtggttcct acactgagtt
     7321 taataagttc tatgcccctg cactgcttca aaaatatgtt atgttggaaa gcataaaacg
     7381 tggaatacct aaatacaact tcctaggcat tcaagggatt tttgatggaa gtgatggtgt
     7441 tttgcgtttt aaacagaatt ttaatggcta tattgtacgc aaagcaggta ctttccgtta
     7501 ccatccatcg cctttaaaat acaaagctat ccagttactc aaaaaaatag taggacgtta
     7561 agatgaaaaa gtcagtattt agatttcttt tagcttcttt tagcttcttt tagtaaaata
     7621 attcttattt gctagaaagg tggagagaca tgcgctggct ttttcgtttg ataggggctt
     7681 tcttttcttt tgtgtggcgt ttgttttggc gtctggtttg gatagttgtg ctcttatgtg
     7741 tgcttgcttt cggacttctc tggtatctga acggggattt tcaaggagcg ctaaagcaag
     7801 cagaacggtc agtaaaaatt ggtcaacaaa gtattgacca atgggagaaa acagggcaac
     7861 tgcctaagtt aagccagaca gatagtcacc agcattctga aggaaggtgg ccacaggcct
     7921 ctgctcgtat ttacctggat ccgcagatgg attcacgctt tcaagaggct tatttagaag
     7981 caatccagaa ctggaatcaa actggtgctt ttaactttga actcgtgact gagtctagta
     8041 aggcggatat tacggctacg gagatgaacg acggaggcac tcctgtggca ggagaggcgg
     8101 aaagtcaaac taatctctta acagggcaat tcttgtccgt aacggtgcgg ttgaatcatt
     8161 attatttgtc caatccatac tatggctact cctatgaacg ccttgtccat acggcagaac
     8221 atgagttagg tcatgcgatt ggcttggacc atacagatga gaagtctgtc atgcaaccag
     8281 caggttcctt ttatggtatc caggaagagg atgttgcaaa cctccgaaaa atatatgaga
     8341 ctagtgagta gggtactatc tttccctact ttttttgcta taatggaact atgaacaact
     8401 tgattaaatc aaaactagag ctcttgccga ccagccctgg ttgctacatt cataaggata
     8461 aaaatggcac cattatctat gtaggaaagg ctaaaaatct gcgtaatcga gtacggtcct
     8521 attttcgtgg aagtcataat accaagacag aggctctggt gtctgaaatt gtggattttg
     8581 aatttattgt tacggagtct aatattgagg cacttctcct agaaattaac ctgatcaagg
     8641 aaaacaagcc caagtacaat atcatgctca aggatgacaa gtcctatcct ttcatcaaaa
     8701 tcaccaatga gcgctatcca cgcttgatta tcactcgtca ggtcaaaaag gacggaggtc
     8761 tttattttgg accctatccc gatgtggggg cagccaatga aatcaagcgg ttgctggatc
     8821 ggatattccc ttttcgtaag tgtaccaacc cgccctctaa ggtctgtttt tattaccata
     8881 tcggtcaatg catggcccac accatctgta agaaggatga ggcttatttc aagtctatgg
     8941 ctcaggaggt gtctgatttt ctgaaaggtc aggatgacaa aatcatcgat gatctcaaga
     9001 gtaaaatggc agtagcagca caaagtatgg agtttgaacg tgcggcggaa taccgtgacc
     9061 tgattcaggc tattggaacg cttcgaacca agcaacgggt catggcgaaa gatttgcaaa
     9121 atcgcgatgt ctttggctac tatgtggata agggctggat gtgtgtgcag gttttctttg
     9181 tccgtcaggg taagcttatc gagcgcgatg tcaatctctt cccctacttc aatgatccag
     9241 atgaggattt tttgacctat gtaggacaat tctatcaaga aaaatctcat ctagttccca
     9301 atgaggtact gattccgcag gatattgacg aagaggctgt caaggctttg gtggatacca
     9361 agattctcaa accccaacgt ggagagaaaa aacagctggt caatctagcc ataaaaaatg
     9421 cacgtgttag tctggagcag aaatttaacc tgctagagaa atctgtcgag aagacccaag
     9481 gagctattga aaatctagga cgtttgctcc aaatcccgac cccagtacgt atcgagtcct
     9541 tcgataactc taatatcatg ggaactagcc ctgtttcggc tatggtggtc tttgtcaacg
     9601 gtaaaccgag taagaaggat taccgtaagt acaagataaa aacggttgtt ggaccagacg
     9661 actatgccag catgagagag gtcattcgca gacgctatgg tcgagtacag cgtgaggctt
     9721 tgactcctcc agatttgatt gtgattgatg gggggcaagg tcaagtcaat atcgctaagc
     9781 aggttatcca agaggaactg ggcttggata ttccaattgc tgggctgcaa aagaatgata
     9841 agcaccaaac ccatgaattg ctctttggag atccgtttga ggtggtggac ttgtctcgca
     9901 attctcagga atttttcctc ctccaacgca tccaagatga ggtgcaccgc tttgccatta
     9961 ccttccaccg ccaactgcgc tccaaaaatt ctttctcatc tcaattggat gggattgacg
    10021 gtctgggacc taaacgcaag cagaatctta tgaagcattt caagtctttg accaaaatca
    10081 aggaagccag tgtggatgag attgtcgaag ttggggtacc tagagtcgtt gcagaggctg
    10141 tgcaaagaaa gttgaacccg cagggagaag ccttgcctca agtagcagaa gaaagagtag
    10201 attaccaaac ggaaggaaac cacaatgaac cataaaatcg caattttatc agatgttcat
    10261 ggcaatgcga cggcgctaga agcagtgatt gcagatgcta aaaatcaagg ggccagtgaa
    10321 tattggcttc tgggagatat ttttcttcct ggtccaggcg caaatgactt agtcgccctg
    10381 ctaaaggacc ttcctatcac agcaagtgtt cgaggcaatt gggatgatcg tgtccttgag
    10441 gctttagatg ggcaatatgg cttagaagac ccacaggaag ttcagctctt gcgtatgacc
    10501 cagtttttga tggagcgaat ggatcctgca acgattgtct ggctacgaag cttgcctttg
    10561 ctggaaaaga aagaaattga cggattgcgc ttttctatct ctcataattt acctgacaaa
    10621 aactatggtg gtgacttgct agttgagaat gatacagaga aatttgacca actactagat
    10681 gcggaaacgg acgtggcagt ttatggtcat gttcacaagc agttgcttcg ttatggaagt
    10741 caagggcaac aaatcatcaa tccagggtcg attggcatgc cctattttaa ttgggaggcg
    10801 ttaaaaaatc accgtgccca gtatgccgtg atagaagttg aagatggaga attactcaat
    10861 atccaatttc gtaaagttgc ttatgattac gaagctgagt tagaattggc caagtccaag
    10921 gggcttccct ttatcgaaat gtatgaagaa ctgcgtcgtg aagataacta tcaggggcac
    10981 aatctggaat tattagccag cttaatagaa aagcatgggt atgtagagga tgtgaaggat
    11041 ttttttgatt ttttgtaaga gtttcctaaa atagccaatg caaactaaaa aagcgattgg
    11101 ctggtccaat cgcttttagt atatcttata ctcaatgaaa atcaaagagc aaactaggaa
    11161 gctagccgta ggttgctcaa agcacagctt tgaggttgca gataaagctg acgtggtttg
    11221 aagagatttt cgaagagtgt tattgtaact gagattgatc tgggaggtaa gaaccaccta
    11281 gataggtatt gctgagtttt tcaagggttc cgtcttgata gagttctttg agcgctttat
    11341 caaattgctc tttaaactct ttttggtcgc ttgagaaaac gatatagttg ctggggctat
    11401 ctgcagaagg taaatcaacg actgagaggt ctaaaccacg gtccttgata atcttttgaa
    11461 cggatacctt gtcaaaaact aggaaatcaa actctccgtt agcaaggtct aggattcgtt
    11521 taccaatatc ctcaccagaa aaattaattg tagcgggatt atcagtgtgt ttctgattcc
    11581 agttattgat gaattgagcg ttagaagttc cggtatcctc ttgtgttgtt ttaccagcga
    11641 tctggtcaag agaagtcaaa ggatttttct tgttgctgac aaggacgagg ggattgttgg
    11701 aaattggaag cgagtaaagg tatttttcag cacgctcttt tgtgtaactc aagttattgg
    11761 ccgcagcctg atagtgacca gaatcaagtc ctgggaagat gctctcccag gcggttcttt
    11821 ggaattgaat ctcgtagtcg ctgagttttt catctactgc ctttaaaact tcgatatcga
    11881 agcctgtcag attgcccttg tcttcgtagt caaatggtgg cacgtcgcca gctgtagcaa
    11941 ggacgattgt cttttgagcg ctagtctctt tgggtgtagc ttgattccca caggcaacca
    12001 aaaatggtag gatagctagt aataggctaa attttttcat actgtctcca ttcaaatgta
    12061 aagtacttgc tagtttatca gaaactttcc tgataaacca atatatatct tttatgtctg
    12121 tgatagaaaa acttaatcat gaaaaatccc tgcaagctct ttcaaattat ggtagaatgg
    12181 aagcagtaga attagaaaag gaaatcgatt atgaaatttc ttgaattaaa taaaaaacgt
    12241 catgcgacta agcattttac tgataagcct gttgatccca aagatgtgcg tacggctatc
    12301 gaaattgcaa ccttggcgcc aagcgcccac aacagccagc cttggaaatt tgtggtggta
    12361 cgtgagaaaa atgctgaact ggcaaagtta gcttatggtt ccaattttga acaggtatca
    12421 tcagcgcctg taaccattgc cttgtttaca gatacggact tagccaaacg tgctcgtaag
    12481 attgcccgtg ttggtggtgc taataacttt tctgaagagc aacttcaata ttttatgaaa
    12541 aatctgccag ctgagtttgc ccgttacagt gagcaacaag tcagcgacta cctagctctc
    12601 aatgcaggtt tggttgccat gaacttggtt cttgcattga cagaccaagg aattggttct
    12661 aacattattc ttggttttga caaatcaaaa gttaatgaag ttttggaaat cgaagaccgt
    12721 ttccgcccag aactcttgat cacagtgggt tatacagacg aaaaattgga accaagctac
    12781 cgcttgccag tagatgaaat catcgagaaa agatagaaag aagaaaaaat gacagcaatt
    12841 gattttacag cagaagtaga aaaacgcaaa gaagacctct tggctgactt gtttagcctt
    12901 ttggaaatca attcagaacg tgatgacagc aaggctgatg cccagcatcc atttgggcct
    12961 ggtccagtaa aagccttgga gaaattcctt gaaatcgcag accgcgatgg ctacccaact
    13021 aagaatgttg ataactatgc aggacatttt gagtttggtg atggagaaga agttctcgga
    13081 atctttgccc atatggatgt ggtgcctgct ggtagcggtt gggacacaga cccttacaca
    13141 ccaactatca aagatggtcg cctttatgcg cgcggggctt cggacgataa gggtcctaca
    13201 acagcttgtt actatggttt gaaaatcatc aaagaattgg gtcttccaac ttctaagaaa
    13261 gttcgcttca tcgttggaac agacgaagaa tcaggctggg cagacatgga ctactacttt
    13321 gagcacgtag gacttgccaa accagatttc ggtttctcac cagatgctga atttccaatc
    13381 atcaatggtg aaaaaggaaa tatcacggaa tacctccact ttgcaggaga aaatacaggt
    13441 gttgcccgtc ttcacagctt tacaggtggt ttacgtgaaa atatggtacc agaatcagca
    13501 acagcagtcg tttcaggtga cttggctgac ttgcaagcta aactagatgc ctttgttgca
    13561 gaacacaaac ttagaggaga actccaagaa gaagctggca aatacaaggt gacgatcatt
    13621 ggtaaatcag cccacggtgc tatgcctgct tcaggtgtca atggcgcaac ttaccttgcc
    13681 ctcttcctca gccagtttgg ctttgctggt ccagccaaag actaccttga catcgcaggt
    13741 aaaattctct tgaacgatca tgagggtgaa aatcttaaga ttgctcatgt ggatgaaaag
    13801 atgggtgctc tttctatgaa tgccggcgtc ttccgcttcg atgaaacaag tgctgataat
    13861 accattgccc tcaacatccg ctatccaaaa ggaacaagtc cagaacaaat caagtcaatc
    13921 cttgaaaact tgccagttgt ttctgttagc ctgtctgaac acggtcacac gcctcactat
    13981 gtgccaatgg aagatccact tgtgcaaacc ttgttgaata tctatgaaaa acaaactggc
    14041 cttaaaggtc atgaacaagt catcggtggt ggaacctttg gtcgcttgct agaacgcgga
    14101 gttgcctacg gtgctatgtt cccagactcg attgatacca tgcaccaagc caatgaattt
    14161 atcgccttgg atgatctttt ccgagcagca gcaatttatg ccgaagctat ttacgaattg
    14221 atcaaataaa acgatagaag tctgagatct tatgcttgga cttctttttg gagggaaagt
    14281 agatgtctca aatcgaaaga atcaaacagg ctatcatggc ggattcacag aatgccagct
    14341 atacagagcg tggcattgag cctctctttg cagcgccaaa aactgctcgc atcaatatca
    14401 tcggtcaggc tccgggactt aaaactcaag aagcaggcct ttactggaaa gataaaagtg
    14461 gtgaccgctt gcgggactgg ctaggtgtgg atgaagatac cttttacaat tcaggttatt
    14521 ttgctgtttt gcctatggat ttctactttc caggacatgg caagtcgggt gatcttccgc
    14581 ctcgtacagg ttttgcagaa aaatggcatc cgcagctctt acaggaattg cctgatattc
    14641 agttaaccct cttgattggg caatatgccc aagcctacta tttacaggag aaaatcagtg
    14701 ggaaggtaac ggagagggtg aaacactata aagactatct gccagcctat tttccgctag
    14761 ttcacccatc accacgaaat caaatctgga tggccaaaaa tccttggttt gaggcagaag
    14821 tagtgccaga tttgaaaaaa agaattaaaa ccattttata gtcaatgaaa atcaaagagc
    14881 aaactaggaa gctagtcgta ggctgctcaa agtacagctt tgaggttgca gataaaactg
    14941 acgaagtcgg taacatacgc acggtaaggc gacgctgacg tggtttgaag agattttcga
    15001 agagtattag aagaaaaaga atgaaagaaa tagcctttga cgcattttac cagctttacc
    15061 aaaacgacca gctttctcta gtggatgtga gagaagtgga tgagtttgca gctcttcatt
    15121 tagaaggtgc ccacaaccta ccgcttagtc aattgactga tagttatgat taattggaca
    15181 aagatcgctt gcattatatt atttgcaaat ctggaatgag atcggcgcgt gcttgccaat
    15241 tcctgttaga acaaggttat aatgttatca atgtccaggg tggcatgtta gcctttgaag
    15301 aactttaaaa ttttgcattt ctcctacttg gtgtggactg ggtaggagag ttttattttt
    15361 agataattct tatttttaag aaaattgaaa acatttaata tttgcctcgt gatgcttttt
    15421 tcagactcct aatcgtggta tactaggtca gtattttata aatatgaagg agatttttat
    15481 ggctaaaaaa ggtaccctaa caggtttgct cctgtttgga atattttttg gtgcggggaa
    15541 cttgattttt ccgccttctc taggtgctct atctggagaa cattttcttc ctgccatcgc
    15601 aggttttgtc ttttcaggcg ttggtatcgc cgtcttgacc cttattattg gaacgctaaa
    15661 tcctaaaggg tatatctacg agatttcaac gaagatagcg ccttggtttg cgactcttta
    15721 cctctcagtt ctttacttgt caatcggtcc attctttgct accccacgta ctgctacaac
    15781 agcttacgaa gtagggatta gccccctttt gtcggatgca aataaaggac ttggcttgat
    15841 tgtatttacg gttctgtatt ttgcggcagc ctatttgatt tcgcttaatc catcaaaaat
    15901 cttagaccgc attggacgta ttttaacgcc agtctttgca attttgattg ttatcttggt
    15961 cgttctggga gctatcaaat atggtggaac aagtcctcaa gctgcttcag ctgcttatca
    16021 agcttctgcc tttggtacag gtttcctaga aggttacaat accttggacg cccttgcctc
    16081 agtggccttt agcgtaatcg cagttcaaac cttgaaacaa cttggatttt caagtaagaa
    16141 agaatacatt tcaactattt gggttgttgg tatcgttgtt gcccttgcct tcagcgctct
    16201 ttacatcggt ttaggttttc ttggaaatca tttcccagta ccagctgaag cgatgaaggg
    16261 tggaacacca ggtgtttaca tcttgtcaca agccactcaa gaaatctttg gctcaacagc
    16321 tcaactcttc cttgcagcta tggttaccgt aacctgcttc acaacgactg ttggtttgat
    16381 tgtgtcaaca gctgagttct ttaatgagcg cttcccacaa atcagctaca aggtttatgc
    16441 gacagccttt accttgattg gatttgctat tgccaatttg ggtcttgatg cgattatcaa
    16501 gtactcaatt ccagtactgg ttatcttgta cccaatcacg attgctatcg ttatgattgt
    16561 cattgtcaac aaatttgttg ctctttcaaa accaggtatg cagttgacaa ttgctgtggt
    16621 tacagttatt gccattgcaa gcgtactagg aagctcgttt aaggttgagt ttcttgcaaa
    16681 tcttgttagc gttcttcctt ttgccaaggc atctctccca tggttggtgc cagccattgt
    16741 tggaatcttg ctctcattgg ttctaccaaa caagcaagaa agcgatgttt ttgaaatgga
    16801 ataatcacta atactcttcg aaaatcaaat tcaaaccgcg tcaacgtcgc cttgtcgtac
    16861 tcaagtacag cctgcggcta gtttcctagt ttgctctttg attttcattg agtataaaat
    16921 cacttttgta gtcaagtcta caggagtgat tttctttttt tatccgatga taaatgtgtt
    16981 ataataggta gcaaaagagg tgaagaaatg aatcaaacag tagaatatat caaagaactg
    17041 acagccattg cgtcgccaac aggctttact cgtgagattg cggactattt agtcaagact
    17101 ctagaaggtt ttggttacca gccggttcgc acatccaagg gcggtgtcaa tgtaactatt
    17161 aaaggtcaaa atgatgagca acatcgctat gtgactgccc atgtagatac gcttggtgct
    17221 attgtccgtg ctgtcaaacc agacggccgt ctcaaaatgg accgtatcgg tggctttcct
    17281 tggaacatga ttgaaggtga aaactgtacc gttcatgtgg ctagcacagg tcaaaaggta
    17341 tcaggaacca tcctcatcca ccaaacttct tgccatgtct acaaggatgc aggaactgca
    17401 gaacgcacac aggacaatat ggaagtgcgt ttggatgcaa aagtaagcaa tgaaaaagaa
    17461 actcgtgcct tgggcatcga ggttggcgac tttatcagtt ttgacccgcg gacagtcgtg
    17521 acagagactg gttttatcaa gtcccgtcac ttggatgata aagtcagcgc agcgattttg
    17581 ctcaacctgc ttcgtattta taagaaagag aagattgaat tgccagtaac aactcatttt
    17641 gctttttcag tttttgaaga agtaggacat ggtgctaatt ctaacattcc tgctcaggta
    17701 gtcgaatatc tggctgttga tatgggagct atgggcgatg gccagcaaac agatgagtac
    17761 acagtgtcta tctgtgtcaa ggatgcctca ggcccttatc actatgactt ccgtcaacac
    17821 ttggtgacct tggcgaaaga gcaagatatt ccttttaagc tggacatcta tccattttat
    17881 ggttcggatg cttcagcggc catgtccgca ggggcagagg tcaaacacgc cctccttggc
    17941 gcgggtatcg agtctagtca ttcttatgag cgtactcata ttgactcggt ggtcgcaaca
    18001 gagcgtatgg ttgatgctta tcttaagagc gaggcggtat catagaatgt gcttaatctg
    18061 tcagagaatc gaactcatta aagctgggca aaatccctac tttgtaaaag aactagaaac
    18121 aggctatgtt gttattggtg actaccaata ctttaagggc tataccttat ttttagcaaa
    18181 agatcatgtc acagaactcc atcatatgga aacttctgta aaacttcgct ttttagagga
    18241 aatgagttta gtccaagaag cagtcgccaa agcatttgag gctgaaaaga tgaatattga
    18301 actcctagga aatggcgatg cccacgctca ttggcatctt tttccccgaa gagcaggtga
    18361 tatgaagagt cacggtttga atggccgtgg cccagtctgg tgggtgccct gggaagaaat
    18421 ggcggcagaa gattgccaag tacaatcgcc agagctggaa gagatgatta aaatcttatc
    18481 tcatgaattg gagaagtatt tggcctaaga gagaggaaga aagaaatgaa aaaaagatat
    18541 gtcattttat ctggtttact agcattgacc cttgcagctt gttcgcaaga aaaaaccaaa
    18601 gttgaagaaa acactcaaaa aacggagcaa agcagtcaac cggaaggaac tgtaggtagc
    18661 aaatctcaag cttctagtca gaaaaaggca gaagttagca ataaagggag ctattatagt
    18721 atccaaggga aatacgatga gattatccta gctaataaac gttatccttt gtcaaaagat
    18781 tacaatccag gagaaaatcc aacagccaag gcagagttgc tcaaactaat cgcagctatg
    18841 caggctgagg gctatccaat cagtgaccaa tacagtgggt ttaggagtta tgaaactcag
    18901 gctaagctct accaagacta tgtcaatcaa gatggtaagg aagcagcaga tcgctactca
    18961 gcacgcccag gttacagtga acaccagacg ggtcttgctt ttgatttgat tgggacagat
    19021 ggggatttag ttactgaaga aaaggcagcc cagtggctct tggaccatgc ggctgactat
    19081 ggctttgttg ttcgctatct taaaggcaag gaaaaggaga ctggctacat ggcagaagaa
    19141 tggcatctcc gctacgttgg aaaagaagcc aaagaaattg ctgcaagtgg tctcagtttg
    19201 gaagagtact atggctttga aggcggcgat tatgtcgatt aaaaatgaaa gttttctctt
    19261 gcatttttag ataaatagtg tataatggaa aggtatgtgt aaagcatact tgtgggaggt
    19321 aaaaatctta cattaccgcc aaaaccacaa aggaggattt agaaatggct aaaaaagtcg
    19381 aaaaacttgt aaaattgcaa atccctgctg gtaaagctac accagctcca ccggttggac
    19441 ctgctcttgg tcaagctggt atcaacatca tgggattcac aaaagagttc aacgctcgta
    19501 cagctgacca agctggtatg atcattccag ttgttatctc agtttacgaa gataaatcat
    19561 ttactttcgt tacgaaaaca ccaccagctg ctgttctttt gaaaaaagct gcaggtgttg
    19621 aaaaaggatc aggtacacct aataaaacta aagttgctac agttactcgt gcacaagtac
    19681 aagaaattgc agaaactaag atgccagatt tgaacgcagc aaacgtagag tctgcaatgc
    19741 gtatgatcga aggtactgct cgttctatgg gattcactgt tgttgactaa tcaataacac
    19801 agtagaaaat ctcacagcag tctattagtt gcttttcata ctaggcaagt gactgaggct
    19861 tgtacttggg tacagcaagg gagcttaagg ccgtaggaag agaaaaatag tagactgaaa
    19921 acccgcaaga cttcatcatt tcgagaagtg acgtgggaga tgaaaatcga ttgaaccact
    19981 tacaaggaga atagaaaatg gctaaaaaaa gcaaacaact tcgtgctgct cttgagaaaa
    20041 tcgacagcac aaaagcatac agtgtagaag aagctgtagc acttgcaaaa gaaactaact
    20101 ttgcaaaatt tgatgcaact gtagaagttg cttacaactt gaacatcgac gttaaaaaag
    20161 ctgaccaaca aatccgtgga gcaatggtat tgccaaacgg tactggtaaa acttcacgtg
    20221 ttcttgtttt cgcacgtggt gcaaaagctg aagaagcaaa agctgctggt gcagactttg
    20281 ttggtgaaga tgaccttgtt gctaaaatca acgacggttg gttggacttc gacgtagtta
    20341 tcgctacacc tgatatgatg gctcttgttg gacgtcttgg acgtgtcctt ggaccacgta
    20401 acttgatgcc aaaccctaaa actggtactg taacaatgga tgttgctaaa gcagttgaag
    20461 agtctaaagg tggtaaaatc acttaccgtg ctgaccgtgc aggtaacgtt caagcaatca
    20521 tcggtaaagt atcatttgaa gctgaaaaat tggttgaaaa ctttaaagct ttcaacgaaa
    20581 caatccaaaa agcaaaacca gctacagcta aaggaactta cgtaacaaac ttgactatca
    20641 caactactca aggtgttggt atcaaagttg acgtaaactc actttaatca gtagttaaag
    20701 taatgtaaaa aagttgaaga cgctatgtct caactttttt tgatgtacga cgggcatgtt
    20761 gtatagtaga ttgaaactag aatagtacac atctacttct aaaacattgt tagaaattga
    20821 tttgactgtc ctgatcgatt cgtcctattc ttatttcatt ttattatata tttgaggtgt
    20881 aagtcctcgg tgggcaccta ctaccggtga acccaatagc gattcccaag tctgactatc
    20941 gtgaggtagc ggattaaaac ggtctgggga tagaccgttt taagtctgac gctggaaata
    21001 agaatcgtca gaggaagaga tagcgaaatc gtggctctac gaacaggaac gtgataataa
    21061 ggcgtatata gcggataagg tatctggctg aggcgaccac tatcaattag tagctcagaa
    21121 gtcggtactt aaacgtgcta tatcaaaacc agtccttgaa aaacgtggac tggtttcgtg
    21181 tttggattat taccttgaac gacatgcgtt aaaagttagt tgaaccgccg tatgccgaac
    21241 gacacgtacg gtggtgtgag aggggctaga gattatcccc tactcgattt cgaaatctag
    21301 tggaatgaat ctggaatagt ccatcgagct ttctaatact cttcgaaaat ctcttcaaac
    21361 cacgtcaacg tcgccttgcc gtgcgtatgg ttactgacta cgtcagttct atccacaacc
    21421 tcaaaacagt gttttgagca acctgaggct agtttcctag tctgctcttt ggttttcatt
    21481 gagtataaca cattgttaga agttggttta aattccctaa tcagtttgtt cacatttacc
    21541 ttcgatatat tatatcccat agttaaggtt ggtcatacag atgattatag tcatggagcc
    21601 gtaaaactta gtgtttcttt agttgacaaa gatgtcatga aaaaaatatc tgtaactgta
    21661 ataggatatt ttgaaataaa tatagatgaa aatatcaccg atattctata cgtaaatggt
    21721 actgctattc tttatcatta tttacgttct attgtttcaa tagtttcggc aattgatagc
    21781 agtgaagcaa tgttgctacc taccattaat gttttagagt tactagataa atctcaacct
    21841 tttgaagaag aataatttat tagctcacta aattgagggt aaggaaaagt aaaatcagta
    21901 agaaaaatgt cttgcattat acagcaacct tttgggaatg agtagatgga ttgaataaaa
    21961 tttgattaag agtggatgat ttatctgtag attattattg gacagttagt cttgaagtag
    22021 tctaagaatt aggttataat cagtagaagc cttgctaata atgaggaggt tagtttat
//
LOCUS       DAVLVP010000023        20899 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.023, whole genome shotgun sequence.
ACCESSION   DAVLVP010000023 DAVLVP010000000
VERSION     DAVLVP010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20899
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.023"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..1187
                     /locus_tag="VYN69_000869"
     CDS             <1..1187
                     /locus_tag="VYN69_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001813680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lanthionine synthetase"
                     /protein_id="HEV1533168.1"
                     /translation="MSQGEKIQKIKQLKQVTSDILLNTPDFSSYFEKYNFKEEAENIK
                     SYLLAKSMDKSGRLFPSNEFGEFVSPVSFQHGFGGVLFFMNKYYVEEDENTVKEWLTK
                     LENYEAANFLHGYSLLFGKAGFLFGKAGFLFGILDRYEKTKERYLIDISKRLVDHLMR
                     VYDNISNLDFALGKSGILLSLMKYCTIFDDKKLANFIKNNINDAYSLLESEDNGDIYS
                     NNFAHGRSGAAYVLKAYTDIFGDSRYQNHLQKFSDGISELLEEKLSSFSKLDNLGLSW
                     CDGVSGLILYLCLIDKERYSEIIYKSQLEMVQQYEAMGTSFCHGLSSLLQTTIYNKNQ
                     KVEQFIKKILLTRSYRNNDRLLQFQGEDGINSYFDFGVGNLGIYWTLLGYTFPFELSK
                     GD"
     gene            1189..2427
                     /locus_tag="VYN69_000870"
     CDS             1189..2427
                     /locus_tag="VYN69_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533169.1"
                     /translation="MHIFLKNRAFRQLTVNEWISSFGDTIFYLAFINYVSSYAFAPLA
                     IFLISLSETIPQVLQLFTGVIADFQKNRISKYISILFIKVLLYSGVTLLLTSTDFSLF
                     SVFFICSMNLISDTIGFLAGYMLTPIYIRLINDDMTEAMGFRQSTSSIVRLIGNLSGG
                     VFLGLFSISTLAFVNVLTFLFAFLGSLLIRNRLKKEEEKIEVPPYVGMSSFFQHLKES
                     MKLLMTMEDVMVLLWILSISQAVLMMVEPVSAILLIHHPFMGLSTGQSLAILIMISLL
                     HVILGGLLSGFLSKKISIRLNIYWSLLMESLIVIDFLRGSFLLILLGSAGDAFSAGVL
                     SPRLQAMIFGIIPEELMGSVQSSINVINLLIPAVLSLALVFLATSAGLEVVAFALIIL
                     LLIAAYLVHQMKNLPNQEEV"
     gene            2616..5762
                     /locus_tag="VYN69_000871"
     CDS             2616..5762
                     /locus_tag="VYN69_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000621470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEV1533170.1"
                     /translation="MIRTEVTSMTVPDRLPARMRMDGRSIFQQIKNYFDTENKEYFKH
                     PNTYDGISMHLEPNILTSMEHFDLTGFHCECKDFQNQGVCKHWVAMDLYFRSLPQAIQ
                     ERIGKASHKPSFASQLIPTLPSEELQDELVEEKATAPSLALHGQVEIRNHSLAWTLKL
                     QVEQAPRAYVIKDIAHFIFLIFQKEDYFVSQKIGTIRLSLNQFNQASQNLLLYIKKYF
                     IDRNEHSYFNFSYGINPRDYGRYLETPVSYLNDLVPLFQALDVFQYVTSKAEYPLIFL
                     DDSPFIPEEEIFKVVKSNNHYEIINTAYFGFIIQEKLWIRHNHFHIIKEEHRYFLDKL
                     ATWIYHYQENSPLIFSKENKAELMQVCNIISNYVPISIPDELQIHDFIPTFAFSKTRN
                     EIALNMVWSFGEKQVHSKQDLLTLPYTYQASKARKIYHQLLSAGFKEEFHSLSKIKIV
                     DFFLKELPRFRTLGQVQLDESLEKLLVEDPAVIDIFDDESFLSVQFDFSMISEDEVEK
                     AIQALWNQESHYQTKQGKVLVFDDESLKVAQSLQDLRAKFSDGKIKMHKSRAFSLSET
                     FKDNEHVNFSRDFKKMAYDLTHPEEFDIKPYEVKAKLRSYQKEGVKWLSMLDHYHFGG
                     ILADDMGLGKTLQTITLLEANLKPDQKALILAPASLLYNWKEEFRKFVPHKQVEVAYG
                     SKTERIKQIEKSVTITITSYPSFRSDLEHYQKQSYDYLILDEAQMIKNSQTKTAQALR
                     EFDVKTCYALSGTPIENRLEEIWSIFQIVLPGLLPSKKEFSKLSPQLVAKLIQPFILR
                     RKKDEVLTELPELSEHLYSNELSSSQKTLYLAQLRRMQEMVSGASAYEIKRHKIEILA
                     GLTRLRQICNTPALFLEDYKGDSGKMDSLFELLDTIREKGSRPLIFSQFTSMLDLIEQ
                     ELEKKEMSHFKITGQTPSDKRQEMVNLFNQGEKDCFLISLKAGGTGLNLTGADTVILC
                     DLWWNPAVEMQAIGRSHRLGQTKQVDVYRLITLGTIEEKIQELQESKKELFNTVLEGQ
                     ESRSNLSVDDIKEILGVE"
     gene            complement(6199..8535)
                     /locus_tag="VYN69_000872"
     CDS             complement(6199..8535)
                     /locus_tag="VYN69_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000360031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEV1533171.1"
                     /translation="MDKNKIMGLTQREVKERQAEGLVNDFTASASTSTWQIVKRNVFT
                     LFNALNFAIALALAFVQAWSNLVFFAVICFNAFSGIVTELRAKHMVDKLNLMTKEKVK
                     TIRDGQEVALNPEELVLGDVIRLSAGEQIPSDALVLEGFAEVNEAMLTGESDLVQKEV
                     DGLLLSGSFLASGSVLSQVHHVGADNYAAKLMLEAKTVKPINSRIMKSLDKLAGFTGK
                     IIIPFGLALLLEALLLKGLPLKSSVVNSSTALLGMLPKGIALLTITSLLTAVIKLGLK
                     KVLVQEMYSVETLARVDMLCLDKTGTITQGKMQVEAVLPLTETYGEEAIASILTSYMA
                     HSEDKNPTAQAIRQRFVGDVAYPMISNLPFSSDRKWGAMELEGLGTVFLGAPEMLLDS
                     EVPEAREALERGSRVLVLALSQEKLDHHKPQKPSDIQALALLEILDPIREGAAETLDY
                     LRSQEVGLKIISGDNPVTVSSIAQKAGFADYHSYVDCSKITDEELMAMAEETAIFGRV
                     SPHQKKLIIQTLKKAGHTTAMTGDGVNDILALREADCSIVMAEGDPATRQIANLVLLN
                     SDFNDVPEILFEGRRVVNNIAHIAPIFLIKTIYSFLLAVICIASALLGRSEWILIFPF
                     IPIQITMIDQFVEGFPPFVLTFERNIKPVEPNFLRKSMLRALPSALMVVFSVLFVKIF
                     GTSQGWSELEISTLLYYLLGSIGFLSVFRACMPFTLWRVLLIVWSVGGFLATALFPRI
                     QKLLEISTLTEQTLPVYGVMMLVFTVIFILTSRYQARK"
     gene            8668..9417
                     /locus_tag="VYN69_000873"
     CDS             8668..9417
                     /locus_tag="VYN69_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEV1533172.1"
                     /translation="MFYTYLRGLVVLLLWSINGNAHYHNTDKIPNQDENYILVAPHRT
                     WWDPVYMAFATKPKQFIFMAKKELFTNRIFGWWIRMCGAFPIDRENPSASAIKYPINV
                     LKKSDRSLIMFPSGSRHSNDVKGGAALIAKMAKVRIMPVTYTGPMTLKGLISRERVDM
                     NFGNPIDISDIKKMNDEGIETVANRIQTEFQRLDEETKQWHNDKKPNPLWWFIRIPAL
                     ILAIILAILTIIFSFIASFIWNPDKKREELA"
     gene            complement(9709..10323)
                     /locus_tag="VYN69_000874"
     CDS             complement(9709..10323)
                     /locus_tag="VYN69_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000531723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CadD family cadmium resistance transporter"
                     /protein_id="HEV1533173.1"
                     /translation="MGQTIISAIGVYISTSIDYLIILIILFAQLSQNKQKWHIYAGQY
                     LGTGLLVGASLVAAYVVNFVPEEWMVGLLGLIPIYLGIRFAIVGEDAEEEEEEIIERL
                     EQSKANQLFWTVTLLTIASGGDNLGIYIPYFASLDWSQTLVALLVFVIGIIIFCEISR
                     VLSSIPLIFETIEKYERIIVPLVFILLGLYIMYENGTIETFLIV"
     gene            complement(11138..11407)
                     /gene="rpsO"
                     /locus_tag="VYN69_000875"
     CDS             complement(11138..11407)
                     /gene="rpsO"
                     /locus_tag="VYN69_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1533174.1"
                     /translation="MAISKEKKNEIIAQYARHEGDTGSVEVQVAVLTWEINHLNEHIK
                     QHKKDHATYRGLMKKIGRRRNLLAYLRKNDVNRYRELINSLGLRR"
     gene            complement(11686..12177)
                     /locus_tag="VYN69_000876"
     CDS             complement(11686..12177)
                     /locus_tag="VYN69_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEV1533175.1"
                     /translation="MKLAVIAANGQAGKAIVEEVVKRGHEVTAIVRSENKSQSESIIK
                     KDLFELTKDDLTGFDAVISAFGAYTPDTLPLHSKSIELFNQLLAGTQTRFLVIGGAGS
                     LYIDETKMTRLLDTPDFPEEFKPLAKAQADELDLLRTKNNLNWTFVSPAVDFIPDGEK
                     TGN"
     gene            13036..13257
                     /locus_tag="VYN69_000877"
     CDS             13036..13257
                     /locus_tag="VYN69_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000422778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEV1533176.1"
                     /translation="MELQELVERSWAIRQAYHELEVKHHDSKWTVEEDLLALSNDIGN
                     FQRLVMTKQGRYYDETPYTLEQKLSENIW"
     gene            13417..15360
                     /gene="thrS"
                     /locus_tag="VYN69_000878"
     CDS             13417..15360
                     /gene="thrS"
                     /locus_tag="VYN69_000878"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEV1533177.1"
                     /translation="MINITFPDGAVREFESGVTTFEIAQSISNSLAKKALAGKFNGKL
                     IDTTRAITEDGSIEIVTPDHEDALPILRHSAAHLFAQAARRLFPDIHLGVGPVIEDGF
                     YYDTDNTAGQISNEDLPRIEEEMQKIVKENFPSIREEVTKDEAREIFKNDPYKLELIE
                     EHSEDEGGLTIYRQGEYVDLCRGPHVPSTGRIQIFHLLHVAGAYWRGNSDNAMMQRIY
                     GTAWFDKKDLKNYLQMREEAKERDHRKLGKELDLFMISQEVGQGLPFWLPNGATIRRE
                     LERYIVNKELVSGYQHVYTPPLASVELYKTSGHWDHYQEDMFPTMDMGDGEEFVLRPM
                     NCPHHIQVFKHHVHSYRELPIRIAEIGMMHRYEKSGALTGLQRVREMSLNDGHLFVTP
                     EQIQEEFQRALQLIIDVYEDFNLTDYRFRLSLRDPQDTHKYFDNDEMWENAQTMLRAA
                     LDEMGVDYFEAEGEAAFYGPKLDIQIKTALGKEETLSTIQLDFLLPERFDLKYIGADG
                     EDHRPVMIHRGVISTMERFTAILIENYKGAFPTWLAPHQVTLIPVSNEKHVDYAWEVA
                     KKLRDRGVRADVDERNEKMQFKIRASQTSKIPYQLIVGDKEMEDETVNVRRYGQKETQ
                     TVSVDNFVQAILADIANKSRVEK"
     gene            complement(15409..16383)
                     /locus_tag="VYN69_000879"
     CDS             complement(15409..16383)
                     /locus_tag="VYN69_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1533178.1"
                     /translation="MLDWKQFFLAYLRSRSRLFIYLLSLAFLVLLFQFLFASLGIYFL
                     YFFFLCCFVTILFFTWDILVETQVYRQELLYGEREAKSPLEIALAEKLEAREMELYQQ
                     RSKAERKLTDLLDYYTLWVHQIKTPIAASQLLVAEVVDRQLKQQLEQEIFKIDSYTNL
                     VLQYLRLESFHDDLVLKQVQIEDLVKEIIRKYALFFIQKGLNVNLHDLDKEIVTDKKW
                     LLVVIEQIISNSLKYTKEGGLEIYMDDQELCIKDTGIGIKNSDVLRVFERGFSGYNGR
                     LTQQSSGLGLYLSKKISEELGHQIRIESEVGKGTTVRIQFAQVNLVLE"
     gene            complement(16376..17053)
                     /locus_tag="VYN69_000880"
     CDS             complement(16376..17053)
                     /locus_tag="VYN69_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000548969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1533179.1"
                     /translation="MHKILLIEDDQVIRQQIGKMLSEWGFEVVLVEDFMEVLSLFVQS
                     EPHLVLMDIGLPLFNGYHWCQEIRKISKVPIMFLSSRDQAMDIVMAINMGADDFVTKP
                     FDQQVLLAKVQGLLRRSYEFGRDESLLEYAGVILNTKSMDLHYQGQVLNLTKNEFQIL
                     RVLFEHAGNIVARDDLMRELWNSDFFIDDNTLSVNVARLRKKLEEQGLVGFIETKKGI
                     GYGLKHA"
     gene            17209..18282
                     /locus_tag="VYN69_000881"
     CDS             17209..18282
                     /locus_tag="VYN69_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2974 domain-containing protein"
                     /protein_id="HEV1533180.1"
                     /translation="MANIFDYLKDVAYDSYYDLPLNELDILTLIEITYLSFDNLVSTL
                     PQRLLDLAPQVPREPTMLTSKNRLQLLDELAQHKRFKNCKLSHFINDIDPELQKQFAA
                     MTYRVSLDTYLIVFRGTDDSIIGWKEDFHLTYMKEIPAQKHALRYLKNFFAHHPKQKV
                     ILAGHSKGGNLAIYAASQIEQSLQNQITAVYTFDAPGLHQELTQTAGYQRIMDRSKIF
                     IPQGSIIGMMLEIPAHQIIVQSTALGGIAQHDTFSWQIEDKHFVQLDKTNSDSRQVDT
                     TFKEWVTTVPDEELQLYFDLFFGTILDAGISSINDLASLKALEYIHHLFVQAQSLTPE
                     EKETLGRLTQLLIDTRYQVWKNR"
     gene            complement(18373..18676)
                     /locus_tag="VYN69_000882"
                     /pseudo
     CDS             complement(18373..18676)
                     /locus_tag="VYN69_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000281600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(18745..19182)
                     /locus_tag="VYN69_000883"
     CDS             complement(18745..19182)
                     /locus_tag="VYN69_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004263219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEV1533181.1"
                     /translation="MQIPSRFTIATHMLIIIALEGKESKVTSDFLAASVGVNPVIIRK
                     ILSQLKKAELISVARGTGGTEIVKDLKDISLLDVYQAVECLGKTGQLFSFHDNPNPNC
                     PVGAHIHDVLDQKLERIQLTMEAELGQTSLEKVVADAESQMKD"
     gene            complement(19258..20100)
                     /locus_tag="VYN69_000884"
     CDS             complement(19258..20100)
                     /locus_tag="VYN69_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEV1533182.1"
                     /translation="MTKIALLSDIHGNTTALEAVLADARQIGVDEYWLLGDILMPGTG
                     RRRILDLLDQLPITARVLGNWEDSLWHGVRKELDSTRPSQRYLLRQCQYVLEEISLEE
                     IEVLHNQPLQIHRQFGDLTVGISHHLPDKNWGRELIHTGKQEEFDRLVTHPPCDIAVY
                     GHIHQQLLRYGTGGQLIVNPGSIGQPFFLDAQLRKDLRAQYMILEFDDKGLVDMDFRR
                     VDYDVAAELQLAKDLRLPYFEVYYESLVNGIHHTHHQEFLRELAQKEGCDRELDDWLK
                     SGND"
     gene            20212..20862
                     /locus_tag="VYN69_000885"
     CDS             20212..20862
                     /locus_tag="VYN69_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000188500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEV1533183.1"
                     /translation="MTPNKEDYLKCIYEIGIDLHKITNKEIAARMQVSPPAVTEMIKR
                     MKSENLILKDKECGYLLTDLGLKLVSELYRKHRLIEVFLVHHLDYTSDQIHEEAEVLE
                     HTVSDLFVERLDKLLGFPKTCPHGGTIPAKGELLVEINNLPLADIKEAGAYRLTRVHD
                     SFDILHYLDKHSLHIGDQLQVKQFDGFSNTFTILSNDEDLQVNMDIAKQLYVEKIN"
BASE COUNT         6455 a         4516 c         3849 g         6079 t
ORIGIN      
        1 tgatgagcca gggagaaaaa atccaaaaaa taaagcaatt gaaacaagtt acaagtgaca
       61 ttcttttaaa tactccagat ttttcaagtt attttgaaaa atataatttt aaagaggagg
      121 ctgagaatat aaaatcatac ttactggcta aatctatgga caaatctggt cgtttgtttc
      181 cgtcaaatga atttggtgag ttcgtaagtc ctgtttcttt ccaacatggt tttggaggag
      241 ttttattttt tatgaataaa tattatgttg aggaagatga aaataccgtt aaagaatggc
      301 ttacgaaatt agaaaattat gaagcagcta attttttaca tggttactct ctactatttg
      361 ggaaagcagg tttcctattt gggaaagcag gtttcctatt tggaatccta gataggtatg
      421 aaaaaacaaa ggagagatat ttaatagaca taagtaagag attagtcgat catttaatgc
      481 gggtttatga caatattagt aacttagatt ttgctttagg gaagtctggt atcctactca
      541 gtttgatgaa atattgtacc atttttgatg ataaaaaact agcaaatttt atcaagaaca
      601 atataaatga tgcatatagt ttattagaat ccgaggataa tggagatata tattctaata
      661 attttgctca cggacgttca ggtgctgcct atgtattaaa agcctatact gatatttttg
      721 gagatagtcg ctatcaaaat catttacaaa aattttctga cgggatatct gaattactag
      781 aagagaaact ttcaagcttt tctaaattag ataatttagg tctttcttgg tgtgacggag
      841 tttcaggatt aatattgtat ctttgtttga ttgataaaga aagatactct gagataatct
      901 ataaatcaca acttgaaatg gtgcaacaat atgaagcaat gggaactagt ttttgccatg
      961 gtttaagtag tttattgcaa acaacaatat acaataaaaa tcaaaaggtt gaacagttca
     1021 taaaaaaaat cttattgact cgttcttatc gtaataatga tagattatta caatttcaag
     1081 gggaagatgg aataaacagt tattttgact ttggtgtggg aaatctgggt atttattgga
     1141 ctttactcgg ttatacattc ccatttgaat taagtaaagg agattaatat gcatattttt
     1201 cttaaaaatc gagcttttcg tcagctaaca gtaaatgagt ggatttcaag ttttggagat
     1261 actatttttt acttagcttt tatcaattat gtctcttctt atgcctttgc tcccttagct
     1321 atatttttga ttagcctatc agaaaccatt ccacaggtct tgcaactttt tactggagtt
     1381 atagctgatt ttcaaaaaaa tcgaatcagc aagtatattt ctatcctctt tatcaaggtc
     1441 ctcctttatt ctggagtgac tttgctcctg acaagtaccg acttctctct cttttctgtt
     1501 ttctttattt gcagcatgaa cttgatttca gatacgattg gttttcttgc aggatacatg
     1561 ctgacaccga tttacatacg actaataaac gatgatatga cggaggctat gggttttagg
     1621 cagtccacga gttccattgt acgtttgata ggtaatctca gcggaggagt atttttagga
     1681 ctgtttagca tttctactct agcttttgta aatgttttaa cctttttatt tgcctttcta
     1741 ggaagtctac ttatccgaaa ccgcttgaaa aaagaggaag aaaaaatcga agttcctcct
     1801 tatgtaggaa tgagtagctt tttccagcat ttgaaagagt caatgaaatt attgatgact
     1861 atggaagatg tcatggtctt gctttggatt ttgtccatta gtcaagctgt tttgatgatg
     1921 gtagagcctg tcagtgcgat tctactgatt caccatcctt ttatgggttt gtcaacaggg
     1981 caaagtctgg caattttaat aatgataagc cttcttcatg ttattttagg tggacttctg
     2041 agtggatttc tatctaaaaa aatttctatt cgcctgaata tttattggag tctattgatg
     2101 gagtctctca ttgttataga ttttctacgt ggaagttttt tacttatttt actaggaagt
     2161 gcgggtgatg ctttctcggc aggagtattg agtcctcgct tgcaagccat gatttttgga
     2221 atcattcctg aggagttaat gggatcagtt caatcatcca tcaatgttat caacttactt
     2281 atcccagctg tcctatccct tgctctcgta tttcttgcaa caagtgctgg tctggaagtt
     2341 gttgcttttg ctcttataat tttactactt atagcagcct atctagttca ccagatgaaa
     2401 aacttaccaa accaagaaga agtttagcta gaatgttatg gataagtcag ttttcacttg
     2461 atagtttcta tggaatcgta atctccttgt aattgattgt cgatattaca tacaactatc
     2521 ttgcaactac aaatgtttcc aatccctatc aagatgcaat ttatgtaaga taaatctatt
     2581 cttttcctat aaattctgat aagatagaga aaagaatgat tagaacagag gttacttcaa
     2641 tgacggttcc cgatagacta cctgctcgta tgagaatgga tggccgctct attttccaac
     2701 agataaaaaa ctattttgat actgaaaata aggaatattt taagcacccc aatacctatg
     2761 atggtatttc catgcatctg gagcccaaca tcctgaccag tatggagcac ttcgatttga
     2821 ctggctttca ttgcgaatgt aaggatttcc aaaatcaggg agtctgtaaa cactgggttg
     2881 ccatggatct ttattttcgt agtctccccc aagctattca agaacgcatt ggtaaagcga
     2941 gtcataaacc aagttttgcc agccaactga ttcctacctt gccatctgaa gagctccaag
     3001 atgaactggt agaagaaaaa gctacggcgc cttcacttgc ccttcatgga caagtcgaaa
     3061 tccgaaatca tagcctagcc tggaccttaa aattacaggt agaacaagct cctcgtgcct
     3121 atgttatcaa ggatatagct cacttcattt ttctcatttt ccaaaaggaa gattactttg
     3181 tttcacagaa aatcgggaca atcagattat ccttgaatca gtttaatcaa gcatcccaaa
     3241 acctactcct ctatattaaa aaatatttta tcgatagaaa tgaacattct tatttcaact
     3301 tcagttatgg cattaaccct agagattacg gacgctattt agagactcct gttagctatt
     3361 taaacgacct cgtcccactt tttcaagctt tggatgtctt ccaatatgta acctcaaaag
     3421 ccgaataccc tcttatattc ttagatgata gtccctttat cccggaagag gaaattttta
     3481 aagttgtcaa atctaataac cattatgaaa tcatcaatac agcttatttt ggtttcatta
     3541 ttcaagaaaa actttggatt cgacacaatc acttccacat catcaaagaa gaacaccgtt
     3601 actttttgga taaattagct acttggatct atcattatca agaaaattct cctcttatct
     3661 tttcaaaaga aaataaggct gaactcatgc aagtctgtaa tatcatctca aactatgttc
     3721 ctatcagtat cccagatgaa ttacaaatcc atgactttat ccccaccttt gccttctcca
     3781 agactagaaa tgaaattgcc cttaatatgg tgtggtcctt tggagaaaaa caagtccatt
     3841 ctaaacaaga tctcctaacc ctcccctata cttaccaagc tagcaaagct agaaaaatct
     3901 atcatcaact gctttctgct ggtttcaaag aggaatttca tagtctttcc aaaatcaaaa
     3961 tcgttgattt cttcctaaaa gaactccccc gttttcgtac tctgggtcaa gttcaactag
     4021 atgaatcgct tgaaaaactc ttagtagaag atcctgctgt cattgacatt tttgacgacg
     4081 aaagcttcct ttcagttcag tttgattttt ctatgatttc agaagatgag gttgaaaaag
     4141 ctatccaagc tttatggaat caagaaagcc actaccaaac caagcaagga aaggttctag
     4201 tctttgacga tgaaagtctc aaggttgctc aaagtctcca agatcttcgc gctaaatttt
     4261 cagacggaaa aatcaagatg cacaaatctc gtgcctttag cctttctgaa accttcaaag
     4321 acaatgaaca tgtcaatttt tctcgtgatt tcaaaaagat ggcttatgac ctgacccacc
     4381 ctgaagaatt tgatatcaag ccttatgaag tcaaggccaa actccgctcc tatcaaaaag
     4441 aaggagtcaa gtggctgagt atgctggatc attatcattt tgggggaatc ctggcagacg
     4501 acatgggtct agggaaaacc ttgcaaacca tcactcttct ggaagccaac cttaaaccag
     4561 accagaaagc tcttatccta gctccagcca gccttcttta taattggaag gaagaattcc
     4621 gaaaatttgt gcctcacaaa caggttgaag tagcctatgg tagcaaaact gagcgtatca
     4681 aacaaattga aaaatctgtt actatcacta ttacaagcta tccatctttt cgatctgacc
     4741 ttgaacatta tcaaaagcag tcttacgact acctcatcct agatgaagca cagatgataa
     4801 aaaatagtca gaccaaaaca gcacaagctt taagagaatt tgatgtcaaa acctgctatg
     4861 ccctatctgg aactcctatt gaaaaccgtc tcgaagagat ttggtctatt ttccagatag
     4921 tattaccagg tctcctacca agtaaaaagg aattttcaaa attgtcacct caactcgttg
     4981 ctaaactgat tcaacctttt attctgcgta ggaaaaaaga cgaagttctg acagaattgc
     5041 ctgaactgag cgagcacctc tacagcaatg aactcagcag ttcgcaaaaa acgctctatc
     5101 tagctcaact tcgacgcatg caagaaatgg tttcaggagc cagcgcctat gagattaaac
     5161 gtcataaaat tgaaatctta gcaggactga cccgacttag acaaatctgt aatacccctg
     5221 ctctcttttt agaagactac aaaggggata gtggaaaaat ggacagctta ttcgagctcc
     5281 tagacaccat tcgtgaaaaa gggtcacgac cacttatctt ttcacaattc acctctatgc
     5341 ttgatctcat tgagcaagaa ctagagaaaa aagaaatgtc tcactttaaa ataacaggac
     5401 aaacaccttc agataaacgt caagaaatgg tcaatctttt caatcaaggg gaaaaagact
     5461 gcttcctcat ttccctaaaa gcaggtggaa caggactgaa tctaacagga gctgataccg
     5521 tcatcctctg tgacctctgg tggaatccag cagttgagat gcaagccatc ggccgaagcc
     5581 accgccttgg ccagaccaaa caagtcgatg tctatcgcct tatcacactt ggtaccatag
     5641 aggaaaaaat acaagaactc caagaaagta aaaaagaact cttcaacacc gtccttgaag
     5701 gacaagaaag cagaagcaat ctgagcgttg atgatattaa agaaatcttg ggtgttgaat
     5761 aaacgaaaaa gaactagagt aaccacactt agtggattta tctagttctt ttttaggttc
     5821 tagactttcc ttgccatcct atgagctcta tacgccttac catcacgttt ctgttcgttt
     5881 agatacaatt tcgccacccc tttatctgac agtttttata ctatcaaact agggagctga
     5941 ccacaagttg ctcaaagtac cgctttgaga ttgtggatag aacggacctg gtttgaagat
     6001 attttcgaag agaattattt cttgcatcag acttcaaata gtctaactac agatatttga
     6061 atctattacc tcaatccaag caggcttgag aatcactctt gggttcaccg atagataata
     6121 gacacgtagg ccttgcacat cagatctacg atagatccat cgtataaaaa actgcaatcc
     6181 acagattgca gtctttcttt attttctagc ttgatagcga ctagtcagga tgaaaatcac
     6241 tgtaaagacc aacatcatga caccataaac aggcaacgtt tgttctgtca aggttgaaat
     6301 ttcaagcagt ttttggattc ttgggaagag agctgtggct aggaagcctc ctactgacca
     6361 gacaatcaag aggactcgcc atagagtgaa tggcatgcag gctctaaata cggataagaa
     6421 accaattgac cccaagagat aatagaggag agttgagatt tctaactcag accaaccttg
     6481 actcgttcca aagattttca caaaaagaac gctaaacacg accatgaggg cacttggtag
     6541 ggcacgaagc atggattttc tgaggaaatt tggctcaaca ggtttgatat ttcgctcaaa
     6601 agtcagaacg aatggtggga aaccttccac aaactggtca atcatggtaa tctggatcgg
     6661 aatgaagggg aaaatcaaaa tccactctga ccgacctagt aaagcactgg caatacagat
     6721 gactgctaac aggaaggaat agatggtctt tatcaagaaa atcggggcga tgtgggcaat
     6781 gttattgacc acgcgacgac cctcgaagag aatctcagga acatcattaa agtctgagtt
     6841 caagagaacc agattggcaa tctgacgggt tgctggatcc ccctccgcca tcacgataga
     6901 acaatccgcc tcacgaaggg ccaagatatc attaaccccg tcccctgtca tagccgttgt
     6961 atgacccgct tttttcaacg tttggatgat gagtttcttt tgatgagggg aaacacgtcc
     7021 gaaaatagct gtctcctccg ccatggccat caattcctca tcggtgattt ttgagcaatc
     7081 tacatagctg tgatagtccg caaaaccagc cttctgggca atgctggaca ccgtaactgg
     7141 attgtcacca gagataatct tgagtcccac ctcctgagaa cggagatagt ccagcgtctc
     7201 tgctgctccc tctcgaatgg ggtccaagat ttccagcaag gctagagcct gaatatcaga
     7261 tggtttctgt ggtttgtgat ggtctaattt ctcctgactg agagctaaga ccaagacacg
     7321 tgatcctctc tccaaggcct ccctggcttc tgggacttca gaatccagca acatctcagg
     7381 tgcccctaag aaaactgtcc ccaagccttc taactccata gccccccact tgcggtcgct
     7441 cgagaaggga agattggaaa tcataggata agcaacatct cccacaaaac gctggcgaat
     7501 ggcttgggca gttggattct tatcctcact atgggccatg tagctagtca agatgctggc
     7561 aatagcctct tcaccgtaag tttccgtcag tggaagaaca gcctccacct gcatctttcc
     7621 ttgggtgatg gtgcccgtct tgtccagaca gagcatatcc acgcgcgcca aggtctcaac
     7681 agagtacatc tcctgcacca agaccttttt caagcccaac ttaatcactg cagtcaagag
     7741 cgaagtaatg gtcaaaaggg caattccctt aggcaacatt cccaaaagag ctgtcgacga
     7801 gtttacaacg gatgacttga gaggcaggcc ttttaaaagc aaggcttcca gcaagagagc
     7861 cagaccaaag ggaatgataa tcttcccagt aaaaccagcc aacttgtcca gcgatttcat
     7921 gatacgggag ttgatgggtt taacggtctt agcctcaagc atgagtttgg cagcatagtt
     7981 gtctgcaccg acatggtgaa cttgagataa aactgaccca ctggctagga aacttcctga
     8041 caaaagtaag ccgtcaactt ccttttgcac caaatcactt tctcccgtta acatggcttc
     8101 attgacttcc gcaaagcctt ccaaaaccaa ggcatcacta ggaatctgct ctcctgcaga
     8161 caaacgaatg acatctccta gcactaattc ttcaggatta agagcaactt cctgaccatc
     8221 acggatggtt ttgacctttt ccttggtcat gagattgagc ttgtccacca tgtgtttggc
     8281 tcgtagctcg gtcacaatcc cagaaaaagc gttaaagcag ataacagcaa agaagaccag
     8341 attgctccaa gcctgcacaa aggcaagggc caaagcaatg gcaaagttca aagcgttaaa
     8401 aagggtaaag acatttcgtt taacgatttg ccaagtgctg gtactggctg atgcggtaaa
     8461 gtcattgacc aaaccctcag cctgtctttc cttgacttct ctttgggtta atcccataat
     8521 cttattttta tccataaatt ctattatatc atttttttta acagaattct aatttcatcc
     8581 caaatatgca tctagtcaag ggaaaagttt gccagccctc ctttgataag gtataataat
     8641 aacaagaaaa caatcgaagg agatcgcatg ttttatactt atttgcgtgg attagttgtg
     8701 ttgctcttat ggtccatcaa tggcaatgct cactatcata atactgataa aattcctaat
     8761 caagatgaaa attatatttt agttgcgcct caccgtacct ggtgggatcc tgtttatatg
     8821 gcctttgcga ccaagccaaa acagttcatc tttatggcaa aaaaagaact ctttaccaac
     8881 cgtatctttg gttggtggat tcgtatgtgt ggcgcctttc ccatcgaccg tgaaaatccc
     8941 agcgcctcag ccatcaaata tcctatcaac gttctcaaaa aaagtgaccg ctctctcatc
     9001 atgtttccaa gtggtagccg ccactcaaac gatgtcaagg ggggcgcagc actgattgcc
     9061 aaaatggcca aggtccgtat catgccggtt acctacaccg gtcccatgac tttgaagggc
     9121 ttgattagcc gtgaacgtgt cgatatgaac tttggaaatc caatcgatat ctcagatatc
     9181 aagaaaatga atgatgaagg cattgaaaca gtcgccaatc gtattcaaac agaattccaa
     9241 cgtctggacg aagaaacgaa acaatggcac aatgataaaa aaccaaatcc actctggtgg
     9301 tttatccgca tccctgccct catccttgct attatcctcg ctatcctaac catcatcttt
     9361 agctttatcg caagcttcat ctggaaccca gataagaaaa gagaagaact tgcatagaag
     9421 aaatgaacct tggccaaaca gctaaggttt tcatttatat agtagattgg aactagaata
     9481 gtacacctct acttctaaaa catttttaga aatcgatttg actgtcctga tcgatttgtc
     9541 ctattcttat ttcattttac tatatttata agaatctcct aatcaaatca aaagccactc
     9601 tacaatagag taactttaag attgtccatt tatactcaat gaaaaagccc tctgaaaatc
     9661 agagagctga tttgagctcg ggctaaaatc ctagtgaaac aaaaaaatct acacgatcag
     9721 aaaagtctct atcgtgccat tttcatacat gatgtatagt ccaagtagaa tgaatactaa
     9781 gggcacaatg attcgctcgt atttttcaat tgtctcgaat attaacggaa tagaggataa
     9841 cacccgacta atctcgcaaa agataattat gccgattaca aacacaagca aggccacgag
     9901 ggtctgtgac caatctaacg aagcaaaata aggtatatag atacctaaat tatctccgcc
     9961 agacgcaatt gtcagcaatg taactgtcca aaacagttga tttgccttgc tttgttctaa
    10021 tctttcaata atttcttcct cttcttcttc cgcatcttct ccaacaattg caaagcgaat
    10081 ccctaaatag atagggatta aaccaagcaa tccaaccatc cattcttcag gcacgaaatt
    10141 aacgacataa gcagcaacta aactcgcccc tacaagtaag cctgtgccta gatattgccc
    10201 cgcataaata tgccatttct gtttattctg tgatagctgt gcaaataaaa taattaaaat
    10261 aattaaataa tcgatactgg tggaaatata aacaccaata gcagatatga ttgtctgtcc
    10321 cataaaaaaa actcctgtat tagtcagtaa taaatcagca gattttagga gagcacagac
    10381 acattaattt agctatcgct agtgggtaat gtcgaacgta ggaaagcatt ttactcctcc
    10441 tcaaaacgta gttacaaagt aatttctaac tggaattcag tgactacttc catgtaaagt
    10501 ataacatact atactttact ttgtaaagag tgggctcttc tagggggctt gcatacagct
    10561 actcggcttg aaaagccgag taactgcgca cctttatggt gtaattagct gataccgttt
    10621 gagatttttg tagtctccaa aattgttacc gataaaacct caataaaaaa gccctctgaa
    10681 gtcagagagc tgatttgagc ccgggctaaa atcctagtga aaaagatgaa actccttgtg
    10741 ctcatcgaac actgtgtcat ttccctattt tcatacggat ttttgacgcc cttagcatca
    10801 tgattcttgc tagataaatc gtttatagac taggcgacga ggcgaagatc gtacgaaact
    10861 ttcagtgaaa caaaaaaaat ctccccgaag ggagaaaagc tggtttattt taccttacag
    10921 tgctaggaac cgtaaccaaa gattatactt gagtatatcg aggtaaggtg acaacgcaaa
    10981 aaagctaccc aatttagagt agctttatta tcaagatatg ctcaattgaa cacggcctaa
    11041 gcacttggca aaaaagataa aatctcctag aaactgaagt ttcttcgtca attttcctat
    11101 ttttgctttg tgcttttgac ggcctttgta tcttgaatta gcgacgaagt cctagagagt
    11161 tgatcaactc acggtaacgg ttaacgtcgt ttttacgcaa gtatgcaagc aagttacgac
    11221 ggcgaccgat ttttttcatc aatccacggt aagtagcgtg gtctttttta tgttgtttga
    11281 tgtgttcgtt aaggtgattg atttcccaag taaggacagc aacttgaacc tctactgaac
    11341 ctgtatcacc ttcgtgacgt gcatattgtg caatgatttc attttttttc tcttttgaga
    11401 ttgccatgat gtttctcctt tttatttggc ttcatccgag tgacaggttg gcatgcctgt
    11461 aaccaagaag aagttatttg tctttacgac aatccctatt ttaccaagat ttaaattttc
    11521 tgtcaaacat taatactctc atagttatta tttttcaagt aaagagatac gttcttttat
    11581 atgatgacct ttttcaagtt catctaccaa tccaatagca taatctgcat aactaatctg
    11641 gcttattcct tttccattag tagtaaaaat ttctcctgct agaatttagt ttcctgtttt
    11701 ttcaccatcg ggaataaaat ctacagcagg actgacaaaa gtccagttga gattattttt
    11761 tgtacgaaga agatccaatt catccgcttg agcttttgct agtggcttaa attcttcagg
    11821 aaaatcaggc gtatctaaaa gtcgtgtcat ttttgtttca tctatataaa ggcttcctgc
    11881 tccccctata actagaaaac gtgtctgtgt tccagcaaga agctgattaa atagttcgat
    11941 tgatttgctg tggagcggta gcgtatctgg tgtataagca ccaaacgctg aaataacagc
    12001 atcaaatcca gtaagatcat cttttgtcaa ctcaaataaa tcttttttaa taatagactc
    12061 agattgactt ttgttttcag aacgaacaat agccgttact tcatgtcctc gtttgactac
    12121 ttcttcaaca attgctttcc ccgcttgtcc atttgctgca ataactgcta gtttcatttt
    12181 ttatacctct cttgttgtaa ttattttagt tacagcaatt gtgacactct taataatcaa
    12241 tgtcaatagt cttgcttaat tattatcaaa atatttctac caagaaaact aaccatgatt
    12301 ctagtgaaaa gaaaaatctt ctttgtcaac aaatttactt tcttgtttta aacatgctat
    12361 aataatcata gcaagagatc taagttgtct gtttttttaa aacgaggtga ttatcatgcg
    12421 tagattctat tcccatctcc cctactatct ggtcatatta ttcttttatt ggccacttta
    12481 tgagttgttc ttgctagttg tttctgaccc ccttacacta tataaacaat cttctcttct
    12541 ttacacctct ggtaatcttg attgtatcgt tactctatag ctaccgtttc cgtttctcac
    12601 tttgatggtt agttggtaac ggactgctct tttactttac tatcataacc tttggtgagt
    12661 ttatactaat ttacttgcta atctatgaaa cagttgctct ggtgggcatg gattctggta
    12721 ttagcatcaa gcatattcta caaaaaatga aaaacaaaaa actttcacaa aatccttgaa
    12781 aaatctcaca atcatgctat aataatccat agagacaagt cacttagtcc ctttctacta
    12841 gagagtgcgt ggttgctgga aacgcatagg aagtctaaac tgatactact cttgagtttt
    12901 ttatgaaaac ataaaacggt ggccacgtta gagccaatca gaggtgtccc tctcttttga
    12961 ggtacataaa tgaaggtgga accacgttgc gacgtccttt cgaggatgtc gcattttttt
    13021 attaggatac taattatgga gttgcaagaa ttagtggagc gcagttgggc aatccgacaa
    13081 gcttatcacg aactggaagt taagcatcat gattccaagt ggacggtaga agaagacctc
    13141 ttggctttat ctaatgatat tggaaatttc caacgactgg tgatgacaaa acaaggacgc
    13201 tactatgatg aaacacccta cacactggaa caaaaacttt cagaaaatat ctggtgacta
    13261 ttagaacttt ctcaacgttt ggatatagac attctgacgg aaatggaaaa cttcctctct
    13321 gataaagaaa agcaattgaa cgttaggact tggaagtagt ctgctgataa aaaatcaatg
    13381 cttagaaact atgaaataat aaaaaaggag aacatcatga ttaacattac tttcccagat
    13441 ggcgctgttc gtgaattcga atctggcgta acaacttttg aaattgccca atctatcagc
    13501 aattccctag ctaaaaaagc cttggctggt aaattcaacg gcaaactcat cgacactact
    13561 cgcgctatca ctgaagatgg aagcatcgaa attgtgacac ctgatcacga agatgccctt
    13621 ccaatcttgc gtcactcagc agctcacttg ttcgcccaag cagctcgtcg tcttttccca
    13681 gacattcact tgggagttgg tccagtcatc gaagatggtt tctactacga tactgacaac
    13741 acagctggtc aaatctctaa cgaagacctt cctcgtatcg aagaagaaat gcaaaaaatc
    13801 gtcaaagaaa acttcccatc tattcgtgaa gaagtgacta aagacgaggc acgtgaaatc
    13861 ttcaaaaatg acccttacaa gttggaattg attgaagaac actcagaaga cgaaggcggt
    13921 ttgactatct atcgtcaggg tgaatatgta gacctctgcc gtggacctca cgttccatca
    13981 acaggtcgta tccaaatctt ccaccttctc catgtagctg gtgcgtactg gcgtggaaac
    14041 agcgacaacg ctatgatgca acgtatctac ggtacagctt ggtttgacaa gaaagacttg
    14101 aaaaactacc ttcaaatgcg tgaagaagct aaggaacgtg accaccgtaa acttggtaaa
    14161 gagcttgacc tctttatgat ttcacaagaa gtgggacaag gtttgccatt ctggttgcca
    14221 aatggtgcga ctatccgtcg tgaattggaa cgctacatcg taaacaaaga gttggtttct
    14281 ggctaccaac acgtctacac tccaccactt gcttctgttg agctttacaa gacttctggt
    14341 cactgggatc attaccaaga agacatgttc ccaaccatgg acatgggtga cggggaagaa
    14401 tttgtccttc gtccaatgaa ctgtccgcac cacatccaag ttttcaaaca ccatgttcac
    14461 tcttaccgtg aattgccaat ccgtatcgct gaaatcggta tgatgcaccg ttacgaaaaa
    14521 tctggtgccc tcactggcct tcaacgtgta cgtgaaatgt cactcaacga cggtcaccta
    14581 ttcgttactc cagaacaaat ccaagaagaa ttccaacgtg cccttcagtt gattatcgat
    14641 gtttatgaag acttcaactt gactgactac cgcttccgcc tctctcttcg tgaccctcaa
    14701 gatactcata agtactttga taacgatgag atgtgggaaa atgcccaaac catgcttcgt
    14761 gcagctcttg atgaaatggg cgtggactac tttgaagccg aaggtgaagc agccttctac
    14821 ggaccaaaat tggatatcca gattaaaact gcccttggaa aagaagaaac cctttctact
    14881 atccaacttg atttcttgtt gccagaacgc ttcgacctca aatacatcgg agctgatggc
    14941 gaagatcacc gtccagtcat gatccaccgt ggggttatct caactatgga acgcttcaca
    15001 gctatcttga ttgagaacta caagggggcc ttcccaacat ggctggcacc acaccaagta
    15061 accctcatcc cagtatctaa cgaaaaacac gtggactacg cttgggaagt ggccaagaaa
    15121 ctccgtgacc gcggtgtccg tgcagacgta gatgagcgca atgaaaaaat gcagttcaag
    15181 atccgtgctt cacaaaccag caagattcct taccaattaa ttgttggaga caaagaaatg
    15241 gaagacgaaa cagtcaacgt tcgtcgctac ggccaaaaag aaacacaaac tgtctcagtt
    15301 gataattttg ttcaagctat cctagctgat atcgccaaca aatcacgcgt tgagaaataa
    15361 gagtctagca taaaagcctc caatctggag gctttttctc atctattttt actcaaggac
    15421 taagttcact tgagcaaact gaatccgcac tgtcgttcct tttccgacct cagactcgat
    15481 acgaatctgg tgccccagtt cttcagaaat tttcttagat agataaaggc caagtccaga
    15541 ggactgctgg gtcaaacggc cattgtatcc tgaaaagcca cgttcaaata ctcggaggac
    15601 atcactgttt tttatcccga ttcccgtatc tttgatacaa agctcttggt catccatata
    15661 aatctccaga ccaccttcct tggtgtactt gagactgttt gagatgattt gctcaataac
    15721 cactagcagc cactttttat ccgtcacgat ttctttatca aggtcatgta gattgacatt
    15781 taagcctttt tgaataaaga aaagagcata tttacgaatt atttccttga ccaagtcctc
    15841 aatttgaacc tgctttaaga ccaaatcatc atggaaactt tctaaacgca ggtactgtaa
    15901 aactaggttg gtataggagt cgattttgaa aatttcctgt tctagctgct gcttcagttg
    15961 gcggtcgacc acttctgcaa ctaagagttg actggctgca atgggggtct ttatctgatg
    16021 gacccacaag gtatagtaat ccagcaaatc cgtcagtttt ctttctgctt ttgacctctg
    16081 ctgatagagt tccatctcac gcgcttctaa tttttctgct aaagctattt ccaaaggaga
    16141 cttggcttcc ctctctccat agagaagttc ctggcgatag acctgcgttt ccaccaatat
    16201 gtcccaagtg aaaaataata tggttacaaa gcaacacaag aagaaaaagt agaggaagta
    16261 aattcctaga ctggcaaata aaaactgaaa gagtaagaca agaaatgcca aagaaagcag
    16321 atagataaaa agacgactac gggagcgcag ataggctaga aaaaattgtt tccaatcaag
    16381 catgcttcaa tccgtaccct attcctttct tggtctcgat aaatcctacc aatccctgct
    16441 cctccaactt tttacgcaaa cgagccacat tgacagagag ggtattatca tcaatgaaaa
    16501 agtcactgtt ccaaagttcc cgcatcaggt cgtcacgtgc tacgatgttg cctgcatgct
    16561 caaataacac gcgtaaaatc tggaattcat tcttggtcaa attcaagact tgcccttgat
    16621 aatgtaaatc catggatttg gtattgagga taacaccagc atattccagc aaactctcat
    16681 cacgcccaaa ctcataggaa cgacgcaaca agccctgaac cttagctaaa agaacctgct
    16741 ggtcaaaagg cttggtcaca aagtcatccg cccccatatt gattgccatg acaatatcca
    16801 tagcctggtc tctcgaagaa agaaacatga taggtacctt ggaaatcttg cggatttcct
    16861 gacaccagtg ataaccatta aacaagggca aaccaatatc catgaggacc agatgaggtt
    16921 ccgactgaac aaatagactc aaaacttcca taaagtcttc taccaggacc acttcaaatc
    16981 cccattcaga gagcattttc ccaatctgtt gacgaatgac ctgatcatct tctattaata
    17041 aaatcttgtg catgcgcttc tccttttcca ttattataac agatttttcc atgctagatg
    17101 gtctgaaact gaatttgaaa tagcctgttt ttagccagta caaacaggct atgctactag
    17161 ctaatttgag ggaaatttgc taagataaat aaaaagaaag gagctcttat ggccaatatt
    17221 tttgactatc tgaaagatgt cgcatatgat tcttattacg accttccctt gaatgagtta
    17281 gacattctaa ccttaataga aatcacctac ctctcctttg ataatctggt ctccacactt
    17341 cctcaacgtc ttttagatct agcacctcag gttccaagag agcccaccat gcttactagc
    17401 aaaaatcgcc ttcaattatt agatgaattg gctcaacaca agcgcttcaa aaattgcaaa
    17461 ctctcccatt ttatcaacga catcgaccct gaactgcaaa agcaatttgc ggctatgact
    17521 tatcgtgtca gcctcgatac ctatctgatt gtctttcgtg ggacagatga cagtatcatt
    17581 ggctggaagg aagatttcca cctgacctat atgaaggaaa ttcctgctca aaagcacgcc
    17641 cttcgctatt taaagaactt ttttgcccat catcctaagc aaaaggttat tctggctggg
    17701 cattccaagg gaggaaatct cgctatctat gctgctagcc aaattgagca aagtttgcaa
    17761 aatcagatca cagcagttta tacatttgat gcacctggtc tccatcaaga attgacacag
    17821 actgcgggtt atcaaaggat aatggataga agcaagatat tcattccaca aggttccatt
    17881 atcggtatga tgctggaaat tcctgctcac caaatcatcg ttcagagtac tgccctgggt
    17941 ggcatcgccc agcacgatac ctttagttgg cagattgagg ataagcactt cgttcaactg
    18001 gataagacca acagtgatag ccggcaagta gacacaacct ttaaagaatg ggtgaccaca
    18061 gtccctgacg aagaacttca gctctacttc gacctcttct ttggcactat tcttgatgct
    18121 ggtattagct ctatcaatga cttggcttcc ttaaaggcgc ttgaatacat tcatcatctc
    18181 tttgtccaag ctcaatccct cactccagaa gaaaaagaaa ccttgggtcg ccttacccag
    18241 ttattgattg atactcgtta ccaggtatgg aaaaatagat aatactcttg aaaattaaat
    18301 gtatacaaaa caaaagacct agaatacata ctttcatgtg cattctaagt ctttttaaat
    18361 agaatctaat agtcaataaa aatcaaagag cattgagaga taatggggct tggaacgtcc
    18421 ctctcgcttc aacaaaatga ccccattata gattaaaaag atgccactta gaaaaagcaa
    18481 aaaaaggaag taatacaaag gcaaatacat aaaaagctaa ctgaacattc tcgtatccat
    18541 ttttataaaa aaggtaggat agataaaaat aacttgaaat gagggataat aaaaataata
    18601 ctggattcca caaacttcta ttatacttcc aaaatgacac tataaaggct aatacaattc
    18661 ctataacgag atacatttct tactccttta atagctacat tttatcataa ttatccaaag
    18721 aaaaaaagag ggcatttatc cctcttaatc cttcatctga ctctctgcat cggccacgac
    18781 tttttctaga ctggtttgac caagttctgc ctccatagtc aactgaattc tctccaattt
    18841 ttgatccaaa acatcatgaa tatgagctcc tacagggcaa tttggattcg gattgtcatg
    18901 gaaactgaag agttgacctg tcttaccaag acattctacc gcctgataaa catctaaaag
    18961 actaatatcc ttaaggtcct tgacaatctc tgttccgccc gttccacgcg ctactgaaat
    19021 cagctctgcc ttcttcaact gggacaagat ctttctgata atgacaggat tgaccccgac
    19081 actagcagcc agaaaatcac tggtcacctt gctttccttc ccttcgaggg caatgattat
    19141 cagcatatga gtcgcaatgg taaatctact tggaatttgc atcctcttct cctttttacg
    19201 aggctaccct gcctctactc ttctttttct attattatac cctttttagt tgtaatgtca
    19261 atcgttacca cttttcaacc agtcgtctaa ctcccgatcg cagccctctt tctgagccaa
    19321 ttctctcaaa aattcctgat gatgagtatg gtggatccca ttgaccagac tttcatagta
    19381 aacctcaaaa tagggaagtc tcaggtcttt agccagctgc aattcagctg ccacatcgta
    19441 gtctacccgt cggaagtcca tatctaccag gcctttgtca tcaaactcca aaatcatata
    19501 ctgggcccgc aagtccttcc gtagctgagc gtccaaaaag aaaggttgcc caatcgaacc
    19561 cggattgaca atcaattgcc caccagtccc gtaacgaagc aactgctggt gaatatgtcc
    19621 ataaacagca atatcacagg gaggatgagt caccaagcgg tcaaactcct cttgtttgcc
    19681 agtatgaatc aactctcgcc cccagttctt atcaggcaga tgatggctaa ttcccaccgt
    19741 caaatcccca aactgacgat gaatttgaag aggttgattg tggagcactt caatttcttc
    19801 tagggaaatt tcctctaaaa catactggca ctggcgcaag agatagcgtt gactggggcg
    19861 agtactatcc aattccttac ggacaccatg ccaaagactg tcttcccagt ttcccaaaac
    19921 tctagccgta atcggtagtt gatccaacaa gtccaaaatc cttctacgcc ctgtccctgg
    19981 catgagaata tctcccaaaa gccagtattc atccactcct atctgccgag catctgccaa
    20041 aacagcctcc aaggcggtgg tatttccatg aatatctgaa agaagagcta ttttcgtcat
    20101 atccatctcc tcgttttttc tcttgcaata agtataacat aaaaagtcac agctagagaa
    20161 atctagcttt ttttgatata ctagataaag atattagaca agaggaaacg aatgacccca
    20221 aacaaagaag actatctaaa atgtatttat gaaattggca tagacctgca taagattacc
    20281 aacaaggaaa ttgcggctcg catgcaagtc tctccccctg ccgtaactga aatgatcaaa
    20341 cgaatgaaaa gtgaaaatct catcctaaag gacaaggaat gtggctatct actgactgac
    20401 ctcggtctca aactggtctc tgagctctat cgtaagcacc gcttgattga agtttttcta
    20461 gttcatcatt tagactatac aagtgaccag attcacgagg aagctgaggt cttggaacac
    20521 actgtctctg acctgttcgt ggaaagacta gataaactgc taggtttccc taaaacctgc
    20581 ccccacgggg gaactattcc tgccaaggga gaactactcg ttgaaatcaa taacctccca
    20641 ctagctgata tcaaggaagc tggcgcctac cgcctgactc gggtgcacga tagttttgac
    20701 attctccatt atctggacaa gcactcactt cacatcggtg accagctcca agtcaagcag
    20761 tttgatggct tcagcaatac cttcactatc ctcagtaacg acgaggattt acaagtgaat
    20821 atggacattg caaaacaact ctatgtcgag aaaatcaact aatttctcaa gtcccctacc
    20881 aaccctgaaa gttttattt
//
LOCUS       DAVLVP010000024        20406 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.024, whole genome shotgun sequence.
ACCESSION   DAVLVP010000024 DAVLVP010000000
VERSION     DAVLVP010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20406
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.024"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            76..3177
                     /locus_tag="VYN69_000886"
     CDS             76..3177
                     /locus_tag="VYN69_000886"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEV1533184.1"
                     /translation="MIAQLDTKTVYSFMESVISIEKYVRAAKEYGYTHLAMMDIDNLY
                     GAFDFLEITKKYGIHPLLGLEMTVFVDDQEVNLRFLALSSVGYQQLMKLSTAKMQGEK
                     TWSVLSQYLEDIAVIVPYFDRVESLELGCDYYIGVYPETLASEFHHPILPLYRVNAFE
                     SRDREVLQVLTAIKENLPLREVPLRSRQDVFISASSLEKLFQERFPQALDNLEKLISG
                     ISYDLDTSLKLPRFNPARPAVEELRERAELGLVQKGLTSKEYQDRLDQELSVIHDMGF
                     DDYFLVVWDLLRFGRSNGYYMGMGRGSAVGSLVSYALDITGIDPVEKNLIFERFLNRE
                     RYTMPDIDIDIPDIYRPDFIRYVGNKYGSKHAAQIVTFSTFGAKQALRDVLKRFGVPE
                     YELSAITKKISFRANLKSAYEGNLQFRQQINSKLEYQKAFEIACKIEGYPRQTSVHAA
                     GVVISDQDLTNYIPLKYGDEIPLTQYDAHGVEASGLLKMDFLGLRNLTFVQKMQELLV
                     ETEGIHLKIEEIDLEDKETLDLFASGNTKGIFQFEQPGAIRLLKRVQPVCFEDVVATT
                     SLNRPGASDYINNFVARKHGQEEVTVLDSALEDILAPTYGIMLYQEQVMQVAQRFAGF
                     SLGKADILRRAMGKKDASAMHEMRASFIQGSIEAGHTAEKAGQVFDVMEKFAGYGFNR
                     SHAYAYSALAFQLAYFKTHYPAIFYQIMLNSANSDYVTDALEAGFEVAPLSINTIPYH
                     DKIANKSIYIGLKSIKGLSKDLALWIIEHRPYSNIEDFIAKLPENYLKHPLLEPLVKV
                     GLFDLFEKNRQKVFNNLANLFEFVKELGSLFGDAIYSWQESEDWTEQEKFYMEQELLG
                     IGVSKHPLQAIASKAIYPITPIGNLSENSYAIILVEVRKIKVIRTKKGENMAFLQADD
                     SKKKLDVTLFSDLYRQVGQEIKEGAFYYVKGKIQSRDGRLQMIAQEIREAVAERFWIQ
                     VKNHDSEQEMSRILDQYKGPIPVIIRYEEEQKTIVSPHHFVAKSDELEAKLNGIVMKT
                     IYR"
     gene            3259..4266
                     /gene="pfkA"
                     /locus_tag="VYN69_000887"
     CDS             3259..4266
                     /gene="pfkA"
                     /locus_tag="VYN69_000887"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEV1533185.1"
                     /translation="MKRIAVLTSGGDAPGMNAAIRAVVRQAISEGMEVFGIYDGYAGM
                     VAGEIHPLDAASVGDIISRGGTFLHSARYPEFAQLEGQLKGIEQLKKHGIEGVVVIGG
                     DGSYHGAMRLTEHGFPAIGLPGTIDNDIVGTDFTIGFDTAVTTAMDAIDKIRDTSSSH
                     RRTFVIEVMGRNAGDIALWAGIATGADEIIIPEADFKMEDIVASIKAGYECGKKHNII
                     VLAEGVMSAAEFGQKLKEAGDTSDLRVTELGHIQRGGSPTARDRVLASRMGAHAVKLL
                     KEGIGGVAVGIRNEKMVENPILGTAEEGALFSLTAEGKIVVNNPHKADIELSSLNKSL
                     S"
     gene            4325..5830
                     /gene="pyk"
                     /locus_tag="VYN69_000888"
     CDS             4325..5830
                     /gene="pyk"
                     /locus_tag="VYN69_000888"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEV1533186.1"
                     /translation="MNKRVKIVATLGPAVEIRGGKKFGEDGYWGEKLDVEASAKNIAK
                     LIEAGANTFRFNFSHGDHQEQGERMATVKLAEKIAGKKVGFLLDTKGPEIRTELFEGE
                     AKEYSYKTGEKIRVATKQGIKSTREVIALNVAGALDIYDDVEVGRQVLVDDGKLGLRV
                     VAKDDETREFEVEVENDGIIAKQKGVNIPNTKIPFPALAERDNDDIRFGLEQGINFIA
                     ISFVRTAKDVNEVRAICEETGNGHVQLFAKIENQQGIDNLDEIIEAADGIMIARGDMG
                     IEVPFEMVPVYQKMIIKKVNAAGKVVITATNMLETMTEKPRATRSEVSDVFNAVIDGT
                     DATMLSGESANGKYPLESVTTMATIDKNAQALLNEYGRLDSDSFERNSKTEVMASAVK
                     DATSSMDIKLVVTLTKTGHTARLISKYRPNADILALTFDELTERGLMLNWGVIPMLTD
                     APSSTDDMFEIAERKAVEAGLVESGDDIVIVAGVPVGEAVRTNTMRIRTVR"
     gene            complement(6561..6786)
                     /locus_tag="VYN69_000889"
                     /pseudo
     CDS             complement(6561..6786)
                     /locus_tag="VYN69_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001838792.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            7634..8506
                     /locus_tag="VYN69_000890"
     CDS             7634..8506
                     /locus_tag="VYN69_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000749590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4300 family protein"
                     /protein_id="HEV1533187.1"
                     /translation="MKKSRKLATLGICSALFLGLAACQQQHATSEGTNQRQSSSAKVP
                     WKASYTNLNNQVSTEEVKSLLSAHLDPNSVDAFFNLVNDYNTIVGSTGLSGDFTSFTH
                     TEYDVEKISHLWNQKKGDFVGTNCRINSYCLLKNSVTIPKLEKNDQLLFLDNDAIDKG
                     KVFDSQDKEEFDILFSRVPTEATTDVKVHAEKMETFFSQFQFNEKARMLSVVLHDNLD
                     GEYLFVGHVGVLVPADDGFLFVEKLTFEEPYQAIKFASKEDCYKYLGTKYADYTGEGL
                     AKPFIMDNDKWVKL"
     gene            complement(8639..8996)
                     /locus_tag="VYN69_000891"
                     /pseudo
     CDS             complement(8639..8996)
                     /locus_tag="VYN69_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            9139..9450
                     /locus_tag="VYN69_000892"
     CDS             9139..9450
                     /locus_tag="VYN69_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533188.1"
                     /translation="MELKDFTEKEQEMIKKRLTMSNISDKETTEKILALVPQDLIKRI
                     PFFVRKHATTRTIKRISIEHPELYAVAQTSGEIPEKECEELRQIITTIFEQKMNKHSI
                     K"
     gene            9454..10170
                     /locus_tag="VYN69_000893"
     CDS             9454..10170
                     /locus_tag="VYN69_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1533189.1"
                     /translation="MKKYFIGGLGSNAYHSKDFLQELDSQVYFLNPYEKHLRDETELK
                     SWFKNEIVEEEFICLIGHSLGGDLARYLASEFEEVKKLILLDGGYLDLDKILPLDTEL
                     EETKNYIKSQIVLDLDVLTSKEKSEAKHWSENMEKAVRQSYHWNVEYNRYELAINYEN
                     IEAILRLRRKIQAFKREVGDTLFISPRYPNEATWREEALKELPDYFDTIFLENFGHEL
                     YTQAPKEIASLMNEWLAYFL"
     gene            complement(10299..11114)
                     /locus_tag="VYN69_000894"
     CDS             complement(10299..11114)
                     /locus_tag="VYN69_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000617844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEV1533190.1"
                     /translation="MIRFLVLAGYFELTIYLHLSGKLNQYINMHYSYLAYISMVLSFI
                     LAIVQLYIWMKQVKTHSHLNSRLAKMTSISLLAIPLVIGLTFPTVSLDSQTVSAKGYH
                     FPLSEGTDLAIQTSEGTTSQYLKPDTSSYFSKSAYEKEMRTAADKYLSQDSIQVTNEN
                     YMEVMEAIYDYPDEFEGKTIQFTGFVYNDPSHANSQFLFRFGIIHCIADSGVYGLLTK
                     GNTRQYENNTWITAKGKLVNHYHKELKQNLPTLEIDSFTKVDKPENPYVYRAF"
     gene            complement(11111..12016)
                     /locus_tag="VYN69_000895"
     CDS             complement(11111..12016)
                     /locus_tag="VYN69_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEV1533191.1"
                     /translation="MTAFQQLPSSVLQTGAIFLSIIIEALPFVLIGSIVSGLIEVYIT
                     PDKVYHFLPRNRWGRIFFGTFVGILFPSCECGIVPIINRFLEKKVPSYTAVPFLVTAP
                     VINPIVLFATYSAFGNSFHVALLRALGSIVVAVILGIFLGFFWQEPIQKENRLACHEH
                     DFSHLSPAKKVFQVFVQAIDEFFDTGRYLVFGCLFASVIQVYVPTRILTSISATPLFA
                     ILLLMILAFLLSLCSEADAFIGASLLSSFGLAPVLAFLVIGPMLDIKNILMMKNYLKA
                     RFISHFITIVTLVVLAYSLLIGVIL"
     gene            12428..14557
                     /locus_tag="VYN69_000896"
     CDS             12428..14557
                     /locus_tag="VYN69_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEV1533192.1"
                     /translation="MDKKYEKISQDLGVTLKQIDTVLSLTAEGATIPFIARYRKDMTG
                     SLDEVAIKAIIDLDKSLTNLNDRKEAVLAKIQEQGKLTKELEEAILVAEKLADVEELY
                     LPYKEKRRTKATIAREAGLFPLARLILQNIVDLEKEAEKFVCEGFATGKEALTGAVDI
                     LVEALSEDVTLRSMTYQEVLRHSKLTSQAKDESLDEKQVFQIYYDFSETVGTMQGYRT
                     LALNRGEKLGVLKIGFEHATDRILAFFATRFKVKNAYIDEVVQQSVKKKVLPAIERRI
                     RTELTEKAEEGAIQLFSDNLRNLLLVAPLKGRVVLGFDPAFRTGAKLAVVDATGKMLT
                     TQVIYPVKPASARQIEEAKKDLADLIGQYGVEIIAIGNGTASRESEAFVAEVLKDFPE
                     VSYVIVNESGASVYSASELARQEFPDLTVEKRSAISIARRLQDPLAELVKIDPKSIGV
                     GQYQHDVSQKKLSESLDFVVDTVVNQVGVNVNTASPALLSHVAGLNKTISENIVKYRE
                     EEGKITSRSQIKKVPRLGAKAFEQAAGFLRIPESSNILDNTGVHPENYAAVKELFKCL
                     DIKDLNEEAQSKLKSLSVKEMAQELDLGPETLKDIIADLLKPGRDFRDSFDAPVLRQD
                     VLDIKDLVVGQKLEGVVRNVVDFGAFVDIGIHEDGLIHISHMSRKFIKHPSQVVAVGD
                     LVSVWVKKIDTEREKVNLSLLAPNETD"
     gene            14544..14993
                     /locus_tag="VYN69_000897"
     CDS             14544..14993
                     /locus_tag="VYN69_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000778579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEV1533193.1"
                     /translation="MKLTDYVKQVSLEDFGRPFIHHVQWNRRLRSTGGRFFPKDGHLD
                     FNPKVYQELGLDVFRKIVRHELCHYHLYFQGKGYQHKDRDFKELLKAVDGLRFVPSLP
                     NSNSKPIKLYRCQSCQQTYQRKRRIDTKRYRCGLCRGKLLLVNQPED"
     gene            15052..15324
                     /locus_tag="VYN69_000898"
     CDS             15052..15324
                     /locus_tag="VYN69_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1533194.1"
                     /translation="MNSKFYKMRRNRMISGVLAGLSDKWNFDVTLVRFLFAIFTVANF
                     GLGVIIYIILASIMPTKEEIEAEMYGTGPRKRKEAQAIDDNEGWFW"
     gene            complement(15678..15869)
                     /locus_tag="VYN69_000899"
     CDS             complement(15678..15869)
                     /locus_tag="VYN69_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533195.1"
                     /translation="MKHKEHILIGLLYLLSPFIGQLLVEHTHFIGTEFTGTAYVICWL
                     SVVISIHHFSKNVLSQQQK"
     gene            16297..17055
                     /locus_tag="VYN69_000900"
     CDS             16297..17055
                     /locus_tag="VYN69_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533196.1"
                     /translation="MTLLDVKHVQKIYKTRFQGNQVEALKDIHFTVEKGDYVAIMGES
                     GSGKSTLLNILAMLDKPSRGQVYLNGTDTATIKNSQASSFRREKLGFVFQDFNLLDTL
                     SVKDNILLPLVLSRRPITEMMKKLVMTAENLGINQLQEKYPYEISGGQKQRVAVARAI
                     ITEPEILLADEPTGALDSKSSAALLDVFDEINERGQTILMVTHSTAAASRAKRVLFIK
                     DGILYNQIYRGEKTERQMFQEISDTLTVIASEVN"
     gene            17057..19045
                     /locus_tag="VYN69_000901"
     CDS             17057..19045
                     /locus_tag="VYN69_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1533197.1"
                     /translation="MFRLTNKLAVSNLIKNRKLYYPFALAVLLAVTVTYLFYSLTFNP
                     KIAEIRGGTTIQATLGFGMFVVTLASAIIVLYANSFVMKNRSKELGIYGMLGLEKRHL
                     ISMTFKELVVFGILTVGAGIGIGALFDKLIFAFLLKLMKLKVELVATFQMNVVIAVLL
                     VFGLIFLGLMFLNALRIARMNALQLSREKASGEKRGRFLPLQTILGSISLGIGYYLAL
                     TVTDPLTALTTFFLAVLLVIFGTYLLFNAGITVFLQILKKNKKYYYQPNNLISVSNLI
                     FRMKKNAVGLATIAILSTMVLVTMSAATSIFNSAESFKKVLNPHDFGVSGQNVEKEDL
                     DKLLSQFASDKGYSVKEKEVLRYSNFGIANQEGTKLTIFEKGQNRVQPKTVFMVFDQK
                     DYENMTGQKLSLSGNEVGLFAKNDGLKGQKALTLNDHQFSVKEEFNKDFIVNHVPNKF
                     NILTTDYNYLVVPDLQAFLDQFPDSDIYNQFYGGMNVSISEEEQLKVAEEYEKYLQKF
                     NAQLNTEGNYVYGSTLADASAQMSVLFGGVFFIGIFLSIIFMVGTVLVIYYKQISEGY
                     EDRERFIILQKVGLDQKQIKQTINKQVLTVFFLPLLFAFIHLAFAYHMLSLILKVIGV
                     LDTTMMLIVTLSICAIFLIAYVLIFMITSRSYRKIVQM"
     gene            complement(19087..19731)
                     /locus_tag="VYN69_000902"
     CDS             complement(19087..19731)
                     /locus_tag="VYN69_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000234620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VIT family protein"
                     /protein_id="HEV1533198.1"
                     /translation="MLRAGVLDANDGIISIAGVVIGVASATSNIWIIFLSGFAAILAG
                     AFSMAGGEYVSVSTPKDTEEAVVSREKLLLDQDRELAKKSLDAAYIQNEEFKTSAQLL
                     TNKIFLKNPLKALVEEKYGIEYEEFTNPWHAAISSFVAFFLRSLPPMLSVTIFPSDYR
                     IPATVLIVGVALLLTGYTSARLGKAPTKTAMIRNLAIGLLTMGVTFLLEQLFSI"
     gene            complement(19757..>20406)
                     /locus_tag="VYN69_000903"
     CDS             complement(19757..>20406)
                     /locus_tag="VYN69_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1533199.1"
                     /translation="VGISTIYYWIHHGKLGLSKQDLLYPRKGKALKKQASTNFKPAGQ
                     SIEQRSEAINLRLENGHYEIDTVLLTRAKNYCLLVLTDRKSRHQIIRLIPNKSAEVVN
                     QALKLILKQHKILSITADNGTEFNRLFDVFSEEHIYYAHPYASWERGTNENHNRLIRR
                     WLPKGTKKMTSKEVAFIEKWINNYPKKCLDYKSPREDFWMANLNLKFSVRNKSRN"
BASE COUNT         6219 a         3711 c         4332 g         6144 t
ORIGIN      
        1 aaatttttct caagagaagc tatttatagg tgaaaatggt ataatatagt gagaaggata
       61 gaggagaagt gtaaattgat cgcacaacta gatacaaaaa cagtctatag ttttatggaa
      121 agcgtcattt cgatcgaaaa gtatgtgaga gcagctaaag aatacggcta cactcatttg
      181 gctatgatgg atattgacaa tctttatggc gctttcgact ttctagagat tacaaaaaaa
      241 tacggcattc atcctttgct agggcttgaa atgacagtgt ttgtagatga tcaggaagtg
      301 aatttgcgct ttttagctct atctagtgtg ggctatcagc agttgatgaa gctttcgaca
      361 gccaagatgc agggggagaa aacttggtca gtcctgtccc agtacctgga ggatatcgcg
      421 gtcattgtgc cttattttga tagagttgag tcgttagaac taggctgtga ttactatata
      481 ggggtttatc cagaaacact agcaagcgaa tttcatcatc ctatcttacc tctttatcgg
      541 gtcaacgctt ttgaaagcag ggatagagaa gttcttcaag ttttaacagc gattaaagaa
      601 aatctaccgc tcagagaagt tcccttgcgt tcgagacaag atgtctttat atcagcaagt
      661 tctttagaga aactattcca agagcgtttt ccgcaagctt tggacaattt agaaaagctt
      721 atttcaggca tttcttacga cttggatact agtctgaaac tgcctcgttt taatccagct
      781 agaccagcag tagaggagtt gagagagcgt gctgaactgg ggcttgttca gaaggggttg
      841 actagtaaag aatatcaaga tagactagac caagaattgt ctgttattca tgatatgggc
      901 tttgatgatt atttcttggt tgtttgggat ttgttgcgtt ttggacgatc gaatggctat
      961 tatatgggaa tgggaagggg ttctgcagta ggcagtttgg tttcttatgc cttagacatc
     1021 acggggattg acccagtaga gaaaaatctg atttttgaac gtttcctcaa tcgtgaacgc
     1081 tacacaatgc cagatattga cattgatatc ccagatattt atcgtccaga ttttatcaga
     1141 tatgttggca ataaatatgg tagtaaacat gcggcacaaa tcgttacttt ttcaaccttt
     1201 ggagccaagc aagctctacg agatgtcttg aaacgctttg gtgttcctga gtatgaatta
     1261 tctgcaatta ctaagaaaat cagttttcgt gccaatctta agtcggccta tgagggaaat
     1321 ctccagtttc gtcagcaaat caatagtaag ttagaatacc aaaaagcttt tgagattgct
     1381 tgcaagatag agggctatcc aaggcaaacc tctgtccatg cggctggtgt tgtaattagt
     1441 gaccaagatt tgaccaacta cattcctcta aagtatggtg atgaaattcc actgactcag
     1501 tatgatgctc atggagttga ggctagcgga cttttgaaga tggactttct gggactacga
     1561 aatttgacct ttgtccagaa gatgcaagag ttgcttgttg aaacagaagg tattcacctt
     1621 aaaattgaag aaatagattt ggaagacaaa gaaacgttag atttatttgc ctctggaaat
     1681 acaaaaggta tctttcaatt tgagcaacct ggtgctattc gcttgctcaa acgtgttcaa
     1741 ccagtctgtt ttgaagatgt cgtagcaact acttctctaa atcgaccagg tgcaagtgac
     1801 tacatcaata attttgtggc aaggaagcat gggcaggaag aagtgactgt tctagattca
     1861 gctctggagg atattttggc tccaacttat ggcattatgc tctatcagga gcaggttatg
     1921 caggttgctc agcgatttgc tggatttagt cttgggaagg ccgatatttt gcgtcgagct
     1981 atgggtaaaa aggatgcctc tgctatgcat gagatgaggg cttcctttat tcaaggctcc
     2041 atagaagcag gccatactgc ggaaaaagca gggcaggttt ttgatgttat ggagaagttt
     2101 gcaggctatg gttttaacag gtcacacgcc tatgcctact cagcattggc cttccagttg
     2161 gcttatttta aaacacatta tccagccata ttttatcaga ttatgttgaa ttctgccaac
     2221 agtgattatg taacagatgc cttagaagca ggttttgaag tagccccatt gtccatcaat
     2281 actattccct atcacgataa aattgccaac aagtctatct atataggttt gaaatcgatt
     2341 aaggggctca gcaaggactt ggcgctttgg attattgaac atagacctta ttctaatatt
     2401 gaagatttta tagctaaatt acctgagaat tatctgaaac atcctctgct agaacctttg
     2461 gtaaaagttg gcctttttga tttatttgaa aaaaatcgtc aaaaagtatt taataactta
     2521 gctaatctat ttgaatttgt gaaagagttg ggaagtttgt ttggagatgc tatttatagt
     2581 tggcaggaat cggaagattg gacggaacaa gaaaaatttt atatggaaca agagctttta
     2641 gggataggtg tcagcaaaca tccactacaa gctattgcaa gtaaggctat ttacccgatt
     2701 accccaatcg gaaatttgtc agaaaatagc tatgctatta ttttggttga agttcggaaa
     2761 ataaaagtga ttcgtaccaa aaagggtgaa aatatggcct tcttacaggc agatgatagt
     2821 aagaaaaaat tggatgtcac tctcttttca gacttatatc gtcaggttgg acaggaaata
     2881 aaagagggag ccttctacta tgtaaaagga aaaatacaat cacgtgatgg ccgtctgcaa
     2941 atgattgcac aagaaataag agaagcagtt gctgaacgct tttggattca ggttaaaaat
     3001 catgactcgg agcaagagat gtcacgtatt ttagaccagt ataaaggccc aatcccagtc
     3061 atcatccggt atgaagagga acagaaaacc atcgtttctc cccatcattt tgtagctaaa
     3121 tctgatgaat tagaggcgaa attgaatgga atagttatga aaacgattta tcgctaaaat
     3181 tacggaaaat agaagaattt tcaacgtaaa tgtggtataa tcagtaagaa tgttaaaaga
     3241 aaaaggagca taaccaatat gaaacgtatt gctgttttga ctagtggtgg agacgcccct
     3301 ggtatgaacg ctgccatccg tgcagttgtt cgtcaagcaa tttcagaagg aatggaagtt
     3361 tttggtatct atgacggata tgctggtatg gttgccggtg aaattcatcc cctagatgca
     3421 gcttcagtag gggacatcat ttctcgtggt ggtactttcc ttcactcagc tcgttaccca
     3481 gagttcgctc aacttgaagg gcaacttaaa gggattgagc aattgaaaaa acacggaatt
     3541 gaaggtgtag ttgttatcgg tggtgacgga tcttaccacg gcgctatgcg tttgactgaa
     3601 catggcttcc cagctattgg tcttccaggt acaatcgata acgatatcgt tggtactgac
     3661 tttacaatcg gttttgacac agcggttact actgccatgg acgctatcga taagattcgt
     3721 gatacatcat caagtcaccg tcgtactttt gtaatcgaag ttatgggacg taacgctggt
     3781 gatatcgctc tttgggctgg tattgcaact ggtgctgatg aaatcatcat ccctgaagca
     3841 gacttcaaga tggaagatat cgtagcaagc atcaaagctg gttatgaatg tggtaaaaaa
     3901 cacaatatca tcgtcttagc tgaaggtgtg atgtcagcgg ctgaatttgg tcaaaaactt
     3961 aaagaagctg gagatacaag cgaccttcgt gtaacagaac ttggacatat tcaacgtggt
     4021 ggttctccaa ctgcgcgtga ccgtgttttg gcgtcacgta tgggtgcaca tgctgttaaa
     4081 cttcttaaag aaggtatcgg tggtgttgcg gttggtattc gtaacgaaaa aatggttgaa
     4141 aatccaattc ttggtactgc agaagaaggg gcattgttta gccttactgc agaaggtaag
     4201 attgtggtta acaacccaca caaagccgat attgagctat ctagcttgaa taagagcttg
     4261 tcataattta caatttagtt aataagacca aaaaggtcat atatataaag gagtcacaaa
     4321 aatcatgaac aaacgtgtaa aaatcgttgc aactttgggt cctgcggtag aaatccgtgg
     4381 tggtaaaaaa ttcggtgagg acggatactg gggtgaaaaa cttgatgttg aagcttcagc
     4441 taaaaacatt gctaaattga ttgaagctgg tgctaacaca ttccgattca acttctcaca
     4501 cggcgaccac caagaacaag gtgagcgtat ggcaactgtt aaacttgcgg aaaaaattgc
     4561 aggtaaaaaa gttggtttcc ttcttgatac aaaaggacct gaaatccgta cagaattgtt
     4621 cgaaggtgaa gctaaagaat attcatacaa aactggtgaa aaaattcgtg ttgcaactaa
     4681 acaaggaatc aaatcaactc gtgaagtgat tgcgttgaac gttgctggtg ctcttgatat
     4741 ctatgatgat gttgaagttg gtcgtcaagt tttggttgac gatggtaaac ttggtcttcg
     4801 tgtggttgct aaagatgatg aaactcgtga atttgaagtt gaagttgaaa acgatggtat
     4861 catcgctaaa caaaaaggtg tgaacatccc taacactaaa attcctttcc cagctcttgc
     4921 tgaacgcgat aacgacgata tccgtttcgg tcttgaacaa ggtatcaact tcatcgcaat
     4981 ttcattcgta cgtactgcaa aagatgtgaa cgaagttcgt gcaatctgtg aagaaactgg
     5041 aaacggacat gttcaattgt tcgctaaaat cgaaaaccaa caaggtatcg ataacttaga
     5101 cgaaatcatc gaagcagctg atggtattat gattgctcgt ggtgatatgg gtatcgaagt
     5161 accgttcgaa atggttccag tttatcaaaa aatgattatc aagaaagtca atgctgcagg
     5221 taaagttgtt atcactgcaa caaacatgct tgaaacaatg actgaaaaac cacgtgcaac
     5281 tcgttcagaa gtatcagacg tattcaacgc tgttatcgac ggaactgacg ctacaatgct
     5341 gtcaggcgag tctgcaaacg gtaaataccc actcgagtca gtaactacaa tggctacaat
     5401 cgacaagaac gctcaagctc ttcttaatga atacggacgt cttgattcag attcatttga
     5461 gcgtaactct aagacagaag taatggcttc tgctgttaaa gatgctacta gctcaatgga
     5521 tatcaaattg gttgtaactc ttactaagac aggtcatact gcacgtttga tttctaaata
     5581 ccgtccaaat gctgacatct tagcattgac atttgacgaa ttgacagaac gtggcttgat
     5641 gttgaactgg ggtgttatcc caatgttgac agatgctcca tcttcaactg acgatatgtt
     5701 cgaaatcgct gaacgtaaag cggtagaagc aggtctcgtt gagtcaggcg atgatatcgt
     5761 tatcgttgct ggtgtgccag taggagaagc tgttcgcaca aacacaatgc gtatccgcac
     5821 agtacgttaa gaaaaatata aaaacctatc atatccagct ttagagcttg tgtgataggc
     5881 tttttgtata gagggtaaga aataggcaaa actttcataa tggattgata ctcttcgaaa
     5941 atctcttcaa accacgtcag cgtcgcctta ccgtatatat gttactgact tcgtcagttc
     6001 tatctacaac ctcaaagcag tgctttgagc aacctgcggc tagcttccta gtttgctctt
     6061 tgattttcat tgagtatgaa ataagatatg cacaaattga ttaggaaatt caaatgaatt
     6121 tctacaaatg ttttagcaat cgtaatgtac ttgtctagat tcgatctgat atattttcga
     6181 tttaatgata tggtatttaa aacctccaaa gtagcttgct ccattctttt acttacgtga
     6241 gtgtagatgt tatttactgt tttagcgttt ttgtgttcca ctctaaccat tatagcattc
     6301 ttctcagcta gtgtactaag gagtgtgtgc ctgaaaatat gggaactaag gggctggttt
     6361 atcggtttct ctagtttagt atttgccttt tgcaaagtga tgttaaatgc ctttctctaa
     6421 atttacatat cactattgtt taacaaaatc taatctactt taggtcactt attctttttt
     6481 tgaaatgtag aatgaacttt ttcaaagttt ttcgaatctt ttaaaatctg tttgttttat
     6541 atcgccattc tccccctttt ttaattctcc ctatatagcc tgacaacttt cccgatggta
     6601 cgaatatggt tgctttcgtc taggtggatg tcggggtatt tgggattgag tttttttaag
     6661 gtagccttgg cggagtttct tgacatagtt agcgccgtct acttggaaga tgccgatggt
     6721 attatagtca atctgtgggg tattcttgat aaataggtag tcgctgtttc ttatctttgg
     6781 ctccatggac ttgctgacga cataagcgat tgggtcgtag tcgtctggga taatggaaac
     6841 tccatatcta aatcgttgtc ctgcatcgag cggctatctg cagagataaa ctacctaaca
     6901 cgagagtaag tagtctgtct gtagtcgtcc agtctgatga tttttacgat acttcgtttt
     6961 tctgctcata cagttgcctc tcggcatagg tcaggacttt accttgtctg ggtggtaccc
     7021 gttggtcgta gatagattgg atatcgctag gagaatcctt ttgaactgga ggaaagaggg
     7081 catcaatcaa gctactgaat actttaacta agtcaaatat agtatttttc ttagtagacc
     7141 taaccctttt ttcataattt ctaatggtgt ttttacttat acctatctta gtacccaatt
     7201 cttattgagt ccaactatta ctagtctata ttgttttata gttgattgag tttggaatag
     7261 tacgctgtag ctgctaaaac atttctagga attaatttgc ctttcctaat agagttgttc
     7321 atatcttatt tcaatctatt atgtttttca cctctaacaa tcgcaatctc ttctttatct
     7381 atgaatgaaa tcgatttcta tttttgtaag taaagcataa cacgaaatcc acgaaaatga
     7441 aaacctttgt tgtgttttcg taaaaaattt gttgacagag cacggaacgc ctactataat
     7501 taaatcaagc ttaaggaaat aacaaaaaaa caaatcggag ggaaagacga tgaacacatt
     7561 aaatgagaaa gtaatcaata tctgtaaagc agtagttaaa gaaactttaa tccaagacat
     7621 ttaggagaaa gaaatgaaga aatcaagaaa attagccaca ttaggaatct gttcagcttt
     7681 gtttttagga ttggctgctt gtcaacaaca acatgctact tctgagggga cgaatcaaag
     7741 gcaaagcagt tcagcgaaag ttccatggaa agcttcatac accaacctaa acaaccaggt
     7801 aagtacagaa gaggtcaaat ctctcttatc agctcacttg gatccaaata gtgttgatgc
     7861 attttttaat ctcgttaatg actataatac cattgtcggc tcaactggct tatcaggaga
     7921 tttcacttcc tttactcaca ccgaatacga tgttgagaaa atcagtcatc tctggaatca
     7981 aaagaagggc gattttgttg gaaccaactg ccgtatcaat agttattgtc ttttgaaaaa
     8041 ttcagtcacc attccaaagc ttgaaaagaa tgaccagttg cttttcctag ataatgatgc
     8101 gattgataaa ggaaaggtct ttgattcaca agataaggaa gagtttgata ttctattttc
     8161 gagagttcca actgaggcaa ctacagatgt caaggttcac gctgaaaaga tggaaacatt
     8221 cttctcacaa tttcaattca atgaaaaagc tcgaatgctg tctgtagtct tgcacgacaa
     8281 tctggatggc gagtatctgt ttgtaggtca cgttggggtc ttagtacctg ctgatgacgg
     8341 tttcttattt gtagagaaat tgactttcga agaaccctac caagcgatta aatttgctag
     8401 taaggaagat tgctacaagt atttgggcac caagtatgcg gattatacag gcgagggact
     8461 ggctaagcct tttatcatgg ataatgataa gtgggttaaa ctttaatagt gttatgatat
     8521 gacagtaatc ttaggatttt tatttctatt tttgatgagt tggtgggtta tttcaatcgt
     8581 taatagccag aaaccacgca aaaatgaaac atttattggc tatatccaaa gatacgatat
     8641 tgatgggaat ggctatcaat cattaccgtg tcctcagaac tgagaggagt tcttgaaatc
     8701 gtaaaactac tttgaacaag agttacttca acccattgga tccgacggag taagttgctt
     8761 tcgtggatac caaaaacagc cgcaatttct tcataagtgc ggtattctcg cacatattga
     8821 agagtggcca taagaaggtc ttctaggctt aatttaggtt ttcgtccccc ttttgtgtgt
     8881 ttaagttgat aagcagtttt taatacagct aatatctctt caaaagtggt gcgctgaaca
     8941 ccaacaagac atttaaatcg tgcatcagtt agttgtttac ttgcttcata attcatagaa
     9001 ctactatacc atattttgtt tcgcaggaag tctaatgcta aaatcttatc agtgagaaga
     9061 tttcttaaag tttattcttt gtaatttcat ctgtattagt acataattta acttagagaa
     9121 aataggagga ctaatcgaat ggaattaaaa gattttacag aaaaagaaca ggaaatgatt
     9181 aagaaaaggc ttacaatgtc taatattagt gacaaagaaa ctactgagaa gattctggcg
     9241 ctagtaccac aagacttgat taagcgaatc ccgttttttg tcagaaaaca tgctacaaca
     9301 cgtacgatta aacgcatttc aattgaacac cctgaactct acgctgtagc tcaaacaagt
     9361 ggtgagattc cagaaaaaga atgcgaagaa ttgcgtcaga ttatcacaac tatctttgaa
     9421 caaaagatga ataagcatag tattaagtag agaatgaaaa aatattttat tggcggtttg
     9481 ggaagcaatg cctatcatag caaggatttt cttcaagaac tagattcgca ggtctatttt
     9541 ctaaatccat atgaaaagca tcttcgagat gaaacagaat tgaaatcatg gtttaaaaat
     9601 gagattgtag aggaagaatt tatctgtctg ataggtcatt ctcttggagg agatttagct
     9661 cgttatctcg catcggaatt tgaagaagta aagaaactga ttcttttgga tggtggctat
     9721 ctagatttag ataagatttt acctttggat acagagttag aggaaactaa aaattatatc
     9781 aaatctcaaa ttgttttgga cttagatgtt cttacttcta aagaaaaatc tgaagcaaag
     9841 cattggtcag aaaatatgga gaaagctgta agacagtcct atcactggaa tgttgagtat
     9901 aatagatatg agttggctat aaattatgaa aatatagaag cgatactccg cctacggaga
     9961 aaaatacaag cttttaagag agaagtggga gataccttgt ttattagtcc tcgctatcct
    10021 aatgaagcta catggagaga ggaagcccta aaagaattgc cagactattt tgatactatt
    10081 tttttagaaa actttggcca tgagctttat actcaagcac ctaaagaaat cgctagtcta
    10141 atgaatgagt ggctcgctta ttttctatga gctacttgta ttgctggaaa tggtataaga
    10201 tgaaaatcta caagtcacta attttcatat aacaaaaaac gaacaggctc tttttctgtt
    10261 attcagaaga gaacttgttc gtttttatct tgattttctt aaaaagctct atatacatag
    10321 ggattttctg gtttatcgac tttggtaaag ctgtcgattt ccaaggttgg aaggttttgt
    10381 ttgagttctt tatggtagtg attgaccagt tttcctttgg ctgttatcca agtgttgttt
    10441 tcatactgcc gggtattgcc cttggtcagc aatccataga caccagaatc tgcgatacag
    10501 tggataatgc cgaatcggaa cagaaattga ctattggcat gactggggtc gttatagaca
    10561 aagcctgtaa actggattgt cttgccctca aactcatctg gatagtcgta gatagcctcc
    10621 atgacttcca tatagttttc attagtgacc tgaatactat cttgggataa gtatttatcc
    10681 gccgccgttc gcatttcctt ttcataggct gattttgaaa aataagaact ggtatctggt
    10741 ttcaaatatt ggcttgtcgt cccttcgctt gtctgaatgg ctagatccgt tccttccgat
    10801 agggggaaat gataaccttt agcagaaaca gtctgagaat ccaagctaac agttgggaaa
    10861 gttaagccga tgacaagtgg aatagccaga agagaaatac tcgtcatctt ggctaatcgg
    10921 ctgttcagat gactgtgggt tttgacttgc ttcatccaga tatacaattg aacgatagcc
    10981 aagataaaag aaagcaccat ggagatatag gccagatagg aatagtgcat gttgatgtac
    11041 tggtttagtt tgcccgacag atggaggtaa atagtcagtt caaaatagcc agctaaaact
    11101 aaaaatcgaa tcataggatg actccaatca agagagaata ggctaaaacg accagagtca
    11161 caatcgtaat gaagtgacta ataaatcgtg ctttcaagta atttttcatc ataagaatat
    11221 ttttgatatc cagcattggg ccaatgacga gaaaagccag aactggagcc aaaccgaaac
    11281 tcgagagtag agaagccccg ataaaggcat ccgcctcact acagagcgaa agaagaaagg
    11341 ccaaaatcat caagagcagg atggcaaaaa gaggagtcgc actgatagag gtcagaatcc
    11401 gagtcggaac ataaacctgt ataacagagg caaagagaca gccaaatacc aaataacgtc
    11461 ccgtatcaaa aaactcatca atggcttgca caaagacctg aaaaactttt tttgcaggac
    11521 ttaagtgaga aaaatcatgc tcatgacagg ctagacgatt ttctttttga atcggttctt
    11581 gccagaaaaa tcctagaaaa attcctagta ttacagccac aacgatggaa cccagagctc
    11641 gtaatagggc gacatggaag gagttgccaa aggcagaata ggtcgcaaaa agaacaatgg
    11701 gattgataac aggtgctgtc acaagaaaag gaacggccgt gtaacttgga accttttttt
    11761 ccagaaaacg attgatgatg gggacgattc cacattcaca ggaagggaaa agtataccga
    11821 caaaggtccc aaaaaagatt ctcccccaac gatttcgagg gagaaaatga taaaccttgt
    11881 caggtgtgat ataaacttca atcagccctg agacaatact tcctatcaga acgaagggaa
    11941 gggcttcaat gataatggag agaaaaatgg ctccagtttg aagtacacta gatgggagtt
    12001 gttgaaaagc agtcatctag tttttctttt ctgctttggc ttttttctct ttgtcatcag
    12061 ggaaagtgac ttgtttcaaa tcagggagtt tagcaaactc ttcaaacatc ttgtccaagt
    12121 cgtctcgttt tgtaaatttg acagccatag gtgtacctcg attctaaatt ctacctctat
    12181 tatactatta ttttgcggaa aagggagttt tttaatttgt tttcgatagt tgatactctt
    12241 cgaaaatctc ttcaaaccac gtcagcttcg ccttaccgta gtacggttac tgacttcgtc
    12301 agttctatcc acaacctcaa aacagtattt tgagcagcct acggctagct tcctagtttg
    12361 ctttttgatt tttattgagt atgaaaagga ttggagaaag ccttccagct tgtggtataa
    12421 tagatttatg gataaaaaat atgaaaaaat ctctcaggat ttgggagtga cgttaaagca
    12481 aattgatacc gttctaagtt tgacagctga aggggcgact attcccttta tcgcgcgtta
    12541 tcgcaaggac atgactggta gtctggatga ggtggcgatt aaggctatta ttgatttgga
    12601 taaaagtctg acaaatctca atgaccgtaa ggaagctgtc ttagctaaga ttcaagaaca
    12661 aggtaagttg accaaggaat tggaagaagc tatcttagtt gccgaaaaat tagcagacgt
    12721 tgaagaactc tatcttcctt ataaggaaaa gcgtcgtacc aaggcaacca ttgcccgtga
    12781 agctggactc tttcctcttg ctcgcttgat tttgcagaat atagttgact tagagaaaga
    12841 agctgaaaag ttcgtctgtg aaggatttgc gactggcaag gaagccttga ccggtgcagt
    12901 tgatattttg gtcgaagcct tatcggaaga tgtgaccttg cgttctatga cttatcagga
    12961 agtgctgaga cactctaaac tcacttctca agccaaggat gaaagtcttg atgaaaagca
    13021 ggtttttcag atttattatg atttttcaga gacagttgga actatgcaag gctatcgtac
    13081 cttggctctc aatcgtgggg agaaacttgg tgtcttgaag atcggttttg aacatgcgac
    13141 ggaccgtatt cttgccttct ttgctactcg tttcaaggtg aaaaatgctt atattgatga
    13201 agttgttcag caatccgtta agaaaaaggt cttgcctgct attgagcgtc gtattcggac
    13261 agaattaact gagaaagctg aagagggagc tatccaactt ttttctgaca atctgcgcaa
    13321 tctcctcttg gttgctccac tgaaagggcg cgtggttctt gggtttgacc cagcctttcg
    13381 tacaggtgcc aagctagctg tcgtggatgc aacaggaaaa atgctgacaa ctcaggttat
    13441 ttatcctgtt aaaccagcat cagctcgtca aatcgaagaa gccaagaaag atttagcaga
    13501 tttaattggt caatacggtg tagagattat tgccattgga aatggaacgg ccagtcgtga
    13561 aagtgaagct tttgtagcgg aagttctgaa agatttccct gaagtcagct atgttatcgt
    13621 taatgaaagt ggtgcttctg tctattctgc cagcgaactt gctcgtcagg agtttccaga
    13681 cttgaccgtt gaaaaacgct ctgccatttc tatcgcccgt cgtttgcaag atcctcttgc
    13741 ggaattggtc aaaatcgatc ctaagtcaat tggtgtcggt caataccaac acgatgtcag
    13801 tcagaagaaa ctatctgaga gtctggactt tgttgttgat acagtggtta accaagttgg
    13861 tgtcaatgtc aatacagcta gcccagctct tctttcacac gtagctggac tcaataaaac
    13921 catttctgaa aatatcgtca aataccgtga ggaagaggga aaaatcactt cacgctccca
    13981 aatcaagaaa gttcctcgtt tgggggccaa ggcttttgaa caggctgctg gtttcctccg
    14041 tatccctgaa agtagcaata ttcttgataa tacaggagtt cacccagaaa attacgctgc
    14101 cgttaaggaa ctcttcaaat gcttggacat taaggatctg aatgaagagg cccaaagcaa
    14161 actcaagtcg ctttcagtca aggaaatggc gcaagagtta gatcttggtc cagaaactct
    14221 taaagatatc attgcagatc ttctcaaacc aggtcgagat ttccgtgatt cctttgatgc
    14281 tccagttctc cgtcaagatg tcctagatat caaggactta gtggttggac aaaagctaga
    14341 aggagtggtg cgtaacgttg ttgatttcgg tgcttttgtt gatatcggaa ttcatgagga
    14401 cggtctcatc catatttccc atatgagtcg caagtttatc aaacacccta gtcaagtggt
    14461 ggcagttgga gatttggtga gcgtttgggt taagaaaatt gatactgaac gtgaaaaagt
    14521 taatctgtct ctcctagctc caaatgaaac tgactgatta cgtcaagcag gtttcactag
    14581 aagacttcgg cagacctttt atccatcatg tccagtggaa taggcgtcta cgttcgacag
    14641 gtgggcgatt tttccccaaa gatgggcatt tggattttaa tcccaaggtt tatcaggaac
    14701 tggggttgga cgtttttagg aaaattgtcc gacatgaact ctgtcattat cacctctatt
    14761 ttcaagggaa aggctatcaa cacaaggatc gggattttaa ggaactgttg aaagcagtgg
    14821 atggattacg gtttgtacca tccttgccca atagtaactc caagccaatc aagctctatc
    14881 gttgccaatc ctgccagcaa acttatcagc gtaagcgtag gattgatacc aaacgctatc
    14941 gctgtggact ttgtcgagga aagttgctat tagtaaatca gcctgaggac tgatgaaagc
    15001 cgagcctatc cgtgatatac tatgtctaac ctagcagaaa gaggaaatat catgaacagt
    15061 aagttttata aaatgagacg aaatcgcatg atttcaggag ttttagcagg tctgtcagac
    15121 aagtggaact ttgatgttac ccttgtacgt tttctgtttg ccatttttac agtcgcaaac
    15181 tttggccttg gcgtgattat ctatatcatc ctagcttcta tcatgccaac caaggaagaa
    15241 atcgaagcag aaatgtacgg aacaggccca cgtaaacgca aagaagctca agccattgac
    15301 gataatgaag gctggttttg gtgaggtttt acaaaagatt tggaatacgc aaaaagcttt
    15361 aaatcaatag tctaaactgc ttgatttaaa gcttttttac ttttatactc aataaaaatc
    15421 aaagtgcaaa ctaggaagcc agccgcaggc tgtaatttag tcggcaatgt gaagttgata
    15481 tagtttgcac tttgattttt gaatagtatt aataatattt cgccagcttc atccacaaac
    15541 tatagtggat tggaactaga ataggacacc tatacttcta aaaaattgtt agaaatcgat
    15601 ttgactgtcc tgatcgattt gtcctgttct tatttcattt tactataaaa gcagtaattt
    15661 aaacgcagtt ttttaatcta tttttgctgc tgagacaata catttttgga aaaatggtgg
    15721 atgctgataa caactgataa ccagcaaata acataagcag tgccggtaaa ttctgtaccg
    15781 ataaagtgtg tgtgttcaac taagagctga ccgatgaatg gagagagcag atagagaagt
    15841 ccgatgagta tatgttcttt gtgtttcatt aaagcgcacc ttctttcctt ataggttgaa
    15901 cgacttgcaa gtgttaaatt gaatggtatg ttatctaggt aagcttacta aaatgttagt
    15961 tcccgcttcc tcctttttaa agtaagcctt ttcatactta tattttacaa ctttaaaaat
    16021 aggaaatcaa atcattttct aagtaatttt gcttatattt cccccaatct tacaaaaatg
    16081 taagattaaa gtctcttttg taaggttagg ttatggcaag cggtcttttt ttgatgtaaa
    16141 ctagactcat aaagaacaaa ggagtaacat catgaaacaa atcttacata agaaaactag
    16201 aaaaccaagt cacaaagaaa ttgagcgcat tcagctggga tgcactatga tgcaagctac
    16261 atttcatttg atgggatatt aagaaaggag attctcatga cacttttaga tgtaaaacac
    16321 gttcaaaaaa tttataaaac tcgttttcag ggcaaccaag tagaggccct caaggatatt
    16381 cacttcaccg tagaaaaggg tgactacgtt gctatcatgg gtgagtctgg ttctggtaaa
    16441 tccactctgc tcaatatcct agctatgctg gataaaccaa gtcgtgggca ggtttaccta
    16501 aacggaactg atacagcaac tattaaaaat tcacaggctt ctagtttccg gcgtgaaaag
    16561 ctaggatttg tcttccaaga ctttaacttg ctagatactc tgtctgttaa ggacaatatc
    16621 ttgcttccgc ttgtcttgtc aagaagacct ataacggaga tgatgaagaa attggtgatg
    16681 acagctgaaa atctgggtat caaccaattg caagagaagt acccttacga gatttctggt
    16741 ggtcagaaac agcgtgtagc agtagcccgc gccatcatca cagaacctga aattctcctt
    16801 gcggacgagc caacaggagc ccttgattcc aagtcatctg cagccttact tgatgtcttt
    16861 gatgaaatca atgagcgtgg gcaaaccatc ctcatggtaa cccactcaac agcagctgct
    16921 agcagggcca agcgtgttct ctttatcaaa gacggcattc tttacaatca aatctaccgt
    16981 ggagagaaga cagaacgtca gatgttccaa gaaatctctg ataccttgac tgtcattgca
    17041 agcgaggtga attagtatgt ttcgattaac caataagtta gcggtatcga acttgattaa
    17101 aaaccgcaaa ctctactatc cttttgcgct ggctgttctc ttggcagtca ctgtcaccta
    17161 tctcttttac tccctaacct tcaatcccaa gattgctgaa atccgtggag gaacaaccat
    17221 tcaggctaca cttggatttg gtatgtttgt cgtcaccctt gcgtcagcca ttatcgttct
    17281 ctatgccaat agttttgtca tgaagaaccg ttccaaggaa ctaggaattt atggcatgtt
    17341 gggcttggag aagcgtcatc ttatcagtat gacctttaag gagttagtgg tatttgggat
    17401 tctaactgtt ggagcgggta tcggtattgg agccttgttt gacaagttaa ttttcgcttt
    17461 cctgctcaaa ctaatgaaat tgaaggttga gctggttgct accttccaaa tgaatgttgt
    17521 cattgcagta cttcttgtct ttggattgat tttcctaggc ctcatgttcc tgaatgccct
    17581 tcgaatcgcc cgtatgaatg ccctccagct ctcgcgtgag aaagcaagcg gagagaaaag
    17641 aggtcgtttc ctacctctcc aaacgattct tggttccata agtttaggga ttggctatta
    17701 tcttgccctt acggtaaccg atcctcttac agccctaaca actttcttcc tagctgtttt
    17761 gctggttatc tttggtactt atctattgtt taatgcaggg attacagtct tcctacaaat
    17821 cttaaagaaa aacaagaaat actattacca acctaataac ctcatctctg tttccaactt
    17881 gattttccgt atgaagaaaa atgcggttgg actagcaacc atcgctattt tgtcaacaat
    17941 ggttttggta accatgtcag cagcgacaag cattttcaat tccgcagaaa gctttaaaaa
    18001 agttctaaat cctcatgatt ttggggtttc agggcaaaat gttgaaaaag aagatttgga
    18061 caaactcttg agccagtttg caagtgacaa aggttatagt gtcaaagaga aagaagtact
    18121 tcgttacagt aactttggta ttgcaaatca agaaggaacc aagttaacta tttttgaaaa
    18181 aggacaaaac cgtgtccaac ccaaaacagt tttcatggta tttgaccaaa aagattatga
    18241 aaatatgact ggtcaaaaac tgtctctatc aggaaatgag gtcggtctct ttgccaaaaa
    18301 tgacggactg aaaggacaga aagctctaac tctaaatgat catcaatttt ctgtcaaaga
    18361 agaatttaat aaagatttca ttgtgaacca tgttccaaat aagtttaata tcttgactac
    18421 tgattacaat taccttgttg ttcctgattt acaagccttt ttggatcaat tcccagattc
    18481 ggatatctat aatcagtttt acggtggtat gaatgtaagt ataagtgaag aagagcaact
    18541 caaggtcgct gaagaatatg aaaaatactt acaaaagttt aatgctcaat taaacactga
    18601 aggtaactat gtgtatggta gcactctagc agatgctagt gctcaaatga gtgtcctctt
    18661 tggcggtgtc ttctttattg gtattttcct atccattatc tttatggtcg gaaccgttct
    18721 ggtcatctac tacaaacaaa tttctgaagg ctatgaagac cgtgaacgct ttattatctt
    18781 gcagaaagtc ggtttagatc aaaagcaaat caagcaaacc atcaacaaac aggttttaac
    18841 tgttttcttc cttcctttgc tctttgcctt catacatctc gcctttgcct accatatgct
    18901 tagcctgatt ttaaaagtga ttggtgtact ggatacgact atgatgttga ttgtgacctt
    18961 gtctatctgc gctatcttcc tcatcgccta tgtgctgatt ttcatgatta cttcaagaag
    19021 ttatcgcaag attgtgcaaa tgtaaaaaag atacctcgac ttcaaaatcg aggtatttct
    19081 tgtattctaa atgctgaaaa gttgttcgag caggaaggta actcccatgg tcaagagacc
    19141 aatagcaagg ttccgaatca tagctgtttt ggttggggct tttccaagtc tagcacttgt
    19201 gtaaccagtg agaagaaggg ccacaccgac aataaggacg gtagcaggga tgcggtaatc
    19261 acttggaaaa atggtcactg acagcattgg aggcaaactt ctaaggaaaa aggcaacgaa
    19321 gctagaaatg gcagcgtgcc aaggattggt aaattcttca tactcaatcc catatttttc
    19381 ctctaccaga gccttgagtg gatttttaag aaagatctta ttagtcaaga gttgggcaga
    19441 agttttgaat tcttcatttt ggatataagc agcatcgaga gattttttgg ctagttccct
    19501 atcttggtct agcaagagtt tttctcgcga aacgacagct tcctcggtat cttttggagt
    19561 tgaaacggat acatattctc caccagccat tgaaaaggca ccagctaaga tagccgcaaa
    19621 acctgataaa aagataatcc agatattgct cgtggcactg gcaactccga taaccacacc
    19681 agcaatggaa ataattccat cgttagcatc aagaacaccc gcacgcagga tatttaaacg
    19741 acctgcaaaa tttgaatcaa tttcgtgatt tgtttctgac gctaaatttc aagttcaagt
    19801 tagccatcca gaagtcttct ctgggtgact tgtagtccaa gcatttttta ggatagttgt
    19861 taatccactt ttcgatgaat gcgacttctt tggaagtcat tttcttggtt cccttaggta
    19921 accatctacg aatgagcctg ttgtgattct cattagttcc cctttcccaa gaggcatagg
    19981 ggtgcgcata atagatgtgc tcctcagaaa atacatcaaa caagcgattg aattccgttc
    20041 cattatctgc cgtgatggaa agaatcttgt gttgttttaa gatgagtttt agagcctgat
    20101 tgaccacctc agcactttta tttggaatca atcggatgat ctgatgtcta ctctttcgat
    20161 ccgtcaagac aagcaagcag tagtttttcg ctctcgtaag tagaaccgta tcaatctcat
    20221 aatgcccatt ctccaagcga agattgatag cttcagaccg ctgttcgatg gattgaccag
    20281 caggtttaaa gttggtgcta gcctgtttct taagcgcttt tccttttcta gggtaaagca
    20341 gatcctgttt gcttaacccc aattttccat gatgaatcca atagtaaatg gttgaaattc
    20401 ccacgt
//
LOCUS       DAVLVP010000025        20245 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.025, whole genome shotgun sequence.
ACCESSION   DAVLVP010000025 DAVLVP010000000
VERSION     DAVLVP010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20245
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.025"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(148..609)
                     /locus_tag="VYN69_000904"
     CDS             complement(148..609)
                     /locus_tag="VYN69_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1533200.1"
                     /translation="MGKERRKQGNLERKKRVVKKRKIPLEELKAFVEAHPDAFLREIA
                     ARFDCALPSVWAVLKQIKVILKKTTSFREQKPEKVSEFLDILDNLKDLPIVYIDETGI
                     NRYLYRPYAGAPRGEKVYDKISGRRFERTNEVEQKLNGSFLIRYIDSQIRE"
     gene            complement(576..677)
                     /locus_tag="VYN69_000905"
     CDS             complement(576..677)
                     /locus_tag="VYN69_000905"
                     /inference="COORDINATES: protein motif:HMM:NF013842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533201.1"
                     /translation="MKGGHRHVEAAKVFDVGARTLFTWEKKDVNKGT"
     gene            986..1366
                     /locus_tag="VYN69_000906"
     CDS             986..1366
                     /locus_tag="VYN69_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1533202.1"
                     /translation="MTYLEKWFDFNRRQKEIESLLEETIAQQSEQSLTLKEFYLLYYL
                     DLAEEKSLRQIDLPDKLHLSPSAVSRMVARLEAKNCGLLSRMCCHQDRRSSFICLTND
                     GQKTLASLQKTVEESLEAGLDFLI"
     gene            1465..1686
                     /locus_tag="VYN69_000907"
     CDS             1465..1686
                     /locus_tag="VYN69_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1533203.1"
                     /translation="MIEITYIDASKNERTVTFESYEDFERSQQACLIGVADYYPVQKL
                     TYKGHNLDYHGTYGDIFFYLMKQDLSQYN"
     gene            1703..2017
                     /gene="trxA"
                     /locus_tag="VYN69_000908"
     CDS             1703..2017
                     /gene="trxA"
                     /locus_tag="VYN69_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1533204.1"
                     /translation="MAKAITDATFEQETKDGLVLVDFWATWCGPCRMQGPILDKLSEE
                     LSEDVLKIVKMDVDENPNTARAFGIMSIPTLLFKKDGQVVKQVAGVHTAEQIKAIIAE
                     LS"
     gene            2324..2857
                     /gene="queF"
                     /locus_tag="VYN69_000909"
     CDS             2324..2857
                     /gene="queF"
                     /locus_tag="VYN69_000909"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000828792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HEV1533205.1"
                     /translation="MKRQSALVVFSGGQDSTTCLFWTMQHYETVEAVTFAYGQRHHLE
                     IQIAREIAKEQGIRHHILDMSLLGQITAQPDFATIHISYIPDKLCVESKSLKLYLFSY
                     RNHGDFHENCINTIGKDLVNLLDPRYLEVWGKFTPRGGISIDPYYNYGKQGTKYEGLA
                     EQRLFQHDLYPEKIDNR"
     gene            2854..2943
                     /locus_tag="VYN69_000910"
                     /pseudo
     CDS             2854..2943
                     /locus_tag="VYN69_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3247..3915)
                     /locus_tag="VYN69_000911"
     CDS             complement(3247..3915)
                     /locus_tag="VYN69_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HEV1533206.1"
                     /translation="MKKFVAELIGTFMLVFVGTGAVVFGNGLDGLGHLGIAFAFGLAI
                     VVAAYSIGTVSGAHLNPAVSIAMFVNKRLSSSELVNYILGQVVGAFIASGAVFFLLGN
                     SGMSTASLGENALANGVTVFGGFLFEVIATFLFVLVIMTVTSESKGNGAIAGLVIGLS
                     LMAMILVGLKITGLSVNPARSLAPAVLVGGAALQQVWIFILAPIAGGVLAALVAKNFL
                     GTEE"
     gene            complement(4026..4580)
                     /locus_tag="VYN69_000912"
     CDS             complement(4026..4580)
                     /locus_tag="VYN69_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2785 domain-containing protein"
                     /protein_id="HEV1533207.1"
                     /translation="MYQDLLRKIAEEKPNYNQEEIQWLFDHLGNPSPEIRDDLSNQGL
                     HYLSKEKDTTDFSSQYGWVHAFAHGADLLTEVVCHPDFPINRIHEVFDILGQLFKRMS
                     IRFTDDEDWRLARVIYEPILQGKLEQEQVASWIKTVDFPIEEREDFYKFSNFRSCLVE
                     VYVQLDQRNSLQDDLKEAIQSFQY"
     gene            complement(4827..6623)
                     /gene="pepF"
                     /locus_tag="VYN69_000913"
     CDS             complement(4827..6623)
                     /gene="pepF"
                     /locus_tag="VYN69_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1533208.1"
                     /translation="MEQKHRSEFPEKELWDLTALYQDREDFLRAIEKAREDINQFSRD
                     YKGNLHIFEDFEKAFAELEQIYIQMSHIGNYGFMPQTTDYSNDEFANIAQAGMEFETD
                     ASVALTFFDDALVVADEEVLDRLGELPHLTAAIRQAKIKKAHYLGADVEKALTNLGEV
                     FYSPQDIYTKMRAGDFEMADFEAHGKTYKNSFVTYENFYQNHEDAEVREKSFRSFSEG
                     LRKHQNTAAAAYLAQVKSEKLLADMKGYDSVFDYLLAEQEVDRVMFDRQIDLIMKDFA
                     PVAQRYLKHVAKVNGLEKMTFADWKLDLDSALNPEVTIDDAYDLVMKSVEPLGQEYCQ
                     EVARYQEERWVDFAANSGKDSGGYAADPYRVHPYVLMSWTGRLSDVYTLIHEIGHSGQ
                     FIFSDNHQSYFNAHMSTYYVEAPSTFNELLLSDYLENQSNDPRQKRFALAHRLTDTYF
                     HNFITHLLEAAFQRKVYTLIEEGETFGASKLNSIMKEVLTDFWGDAIEIDDDAALTWM
                     RQAHYYMGLYSYTYSAGLVISTAGYLHLKNSETGAEDWLNLLKSGGSKTPLESAMIIG
                     ADISTDKPLRDTIQFLSDTVDQIIAYSAELGE"
     gene            complement(6634..7377)
                     /locus_tag="VYN69_000914"
     CDS             complement(6634..7377)
                     /locus_tag="VYN69_000914"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001188214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEV1533209.1"
                     /translation="MQQYFVKGSAISPVTIEDKETSKHMFQVMRLKEEDEVTLVFDDG
                     IKRLARVLDMENRQFELVQELNDNVELPVQVTIASGFPKGDKLEFIAQKVTELGASQI
                     WAFPADWSVAKWDGKKLGKKVEKLEKIALGAAEQSKRNIVPSIKLFEKKADFLAQLDQ
                     FDSIIVAYEESAKEGEAAALLQAVTGLETGAKLLFIFGPEGGLSPAEIESFEAKGAVL
                     AGLGPRILRAETAPLYALSALSVLVELEK"
     gene            complement(7379..8329)
                     /locus_tag="VYN69_000915"
     CDS             complement(7379..8329)
                     /locus_tag="VYN69_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000451122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEV1533210.1"
                     /translation="METWQELKVTVKREGEELVSNLLIELGAQGVAIEDSMDYMGNVD
                     RFGEIFPEVEQQEEIVVTAYYPDTVDVTVVEADLQARLAELTDFMDLGEVKMGTTALA
                     EEDWADNWKKYYEPARITHDLTIVPSWTDYEATAGEKIIKLDPGMAFGTGTHPTTKMS
                     LFALEQVLRGGETVLDVGTGSGVLSIASSLLGAKEIFAYDLDDVAVRVAQENIELNPG
                     MENIHVAAGDLLKGVEIEADVIVANILADILIHLIDDAYRLVKDEGYLIMSGIIKDKW
                     DMVRESAESAGFFLETHMVQGEWNTCVFKKTKDISGVIGG"
     gene            complement(8467..8895)
                     /locus_tag="VYN69_000916"
     CDS             complement(8467..8895)
                     /locus_tag="VYN69_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1533211.1"
                     /translation="MELEISGFPGCKIALFCGDKLLTILRDDKASIPWANMWELPGGG
                     REGDESPFECVAREVYEELGIHLTEDCLLWSKVYPSMLFADKQSVFLVGQLTQNQFDS
                     IVFGDEGQGYQLMNVEEFLSSSQVVPQLQERLKDYLKVSD"
     gene            complement(8861..9964)
                     /locus_tag="VYN69_000917"
     CDS             complement(8861..9964)
                     /locus_tag="VYN69_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HEV1533212.1"
                     /translation="MKKIGTYLVYVLAVVFIMLAFACGTIAFAELGYSAVLAFTFGYA
                     FALLSMYLIFILHELGHAFCGYLTGYRLVAFGLGHFILTKKSGRFHLSRTAILKNVGA
                     QYIGLKEDESDQRIILMLSGGLMVHLGLILLAIVFGFLTRSWYFAGTWIFLNLSFFLN
                     NILPVDITDGAKIWELLQHPENTKYAYLVLRHSAQTLLAPQEYDLKDFVQAVPEDARG
                     SFADGVLGMQGEVSILEGKEEVAKQQFQALLERATTPMMQTVAQLSLLHIALLEGDFE
                     QAEQYASIRQVKSFLSLKLSNLQVIQAWYQLKVKKDVDRTRKALKIARQKMDSSRMLR
                     DEKRYYENLLAELEKEMLEGTEDGTRNFWFPRL"
     gene            complement(9983..10453)
                     /locus_tag="VYN69_000918"
     CDS             complement(9983..10453)
                     /locus_tag="VYN69_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000257105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEV1533213.1"
                     /translation="MATYGFLDVLEEELDKNFPFDFEISWDKRNHAVEVSFLLEAQNA
                     AGVEMVDEDGEVSSDDILFEEAVLFYNPAKSTVNEEDYLTVIPYLPKKGFSREFLAYF
                     SLFLKDTAEVGLDVLMDFLEDPEAEEFIMEWNQEVFEEGKVGLEEGEFYPYPRY"
     gene            complement(10744..11196)
                     /locus_tag="VYN69_000919"
     CDS             complement(10744..11196)
                     /locus_tag="VYN69_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000961815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEV1533214.1"
                     /translation="MLVTYPALFYYDDTDGAEATYFVHFPDFEYSATQGEGISEALAM
                     GSEWLGITVADLIESDGELPQPSDINSLSLIDNDPFKDDEDFVSTYDLDKSFISMVSV
                     DVSEYLGSQEPIKKTLTIPKWADKLGREMGLNFSQTLTDAIADKKVQA"
     gene            complement(11233..11412)
                     /locus_tag="VYN69_000920"
     CDS             complement(11233..11412)
                     /locus_tag="VYN69_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEV1533215.1"
                     /translation="MPMTQKEMVKLLTAHGWIKTRGGKGSHIKMEKQGERPITILHGE
                     LNKYTERGIRKQAGL"
     gene            complement(11549..11737)
                     /locus_tag="VYN69_000921"
     CDS             complement(11549..11737)
                     /locus_tag="VYN69_000921"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533216.1"
                     /translation="MTWFEEIFEEYQFVKIGDKKYRVRSGPMIHSTPPQSVLDRDTFT
                     IDYTDDELLDKEAVFTTH"
     gene            complement(11892..12131)
                     /locus_tag="VYN69_000922"
     CDS             complement(11892..12131)
                     /locus_tag="VYN69_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533217.1"
                     /translation="MKREVISNGNDGPSQEILIFTKQIRHWILSDQVISGKRKLFFRE
                     DTPKEILDLYENIKSKLDFAYQEVHSNNGLKKYEK"
     gene            12401..13672
                     /locus_tag="VYN69_000923"
     CDS             12401..13672
                     /locus_tag="VYN69_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001113185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEV1533218.1"
                     /translation="MPDNLALRMRPKTIDQVIGQEHLVGPGKIIRRMVEANRLSSMIL
                     YGPPGIGKTSIASAIAGTTKYAFRTFNATVDSKKRLQEISEEAKFSGGLVLLLDEIHR
                     LDKTKQDFLLPLLESGLVIMIGATTENPFFSVTPAIRSRVQIFELEPLSNQDVKEALQ
                     IALSNPERGFNFPIELDEDALDFIATSTNGDLRSAFNSLDLAVLSTPENDEGIRHITL
                     DIMENSLQRSYITMDKDGDGHYDVLSALQKSIRGSDVDASLHYTARLIEAGDLPSLAR
                     RLTVIAYEDIGLANPEAQIHTVTALDAAQKIGFPEARILIANVVIDLALSPKSNSAYV
                     AMDKALADLKTSGHLPIPRHLRDGHYSGSKELGNAQDYLYPHNYPGNWVKQDYLPEKI
                     RNHHYFQAEDTGKYERALAQRKEAIDRLRKI"
     gene            13937..14009
                     /locus_tag="VYN69_000924"
     tRNA            13937..14009
                     /locus_tag="VYN69_000924"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13970..13972,aa:Lys,seq:ctt)
     gene            complement(14219..15538)
                     /locus_tag="VYN69_000925"
     CDS             complement(14219..15538)
                     /locus_tag="VYN69_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEV1533219.1"
                     /translation="MINKTFTVEKANRFIAENKHLVNTQYKPEEHFSAEIGWINDPNG
                     FVYFRGEYHLFYQFYPYDSVWGPMHWGHAKSKNLVTWEHLPVALAPDQDYDRNGCFSG
                     SAIVKDDRLWIMYTGHIEEKTGVRQVQNMAFSDDGIHFEKISQNPVATGADLPDELIA
                     ADFRDPKLFEKDGRYYSVVATKHKDNVGCIVLLGSDNLVEWQFESIFLKGVEHQGFMW
                     ECPDYFELDGKDCLIMSPMRYQREGDSYHNINSSLLFTGKVEWGEKCFIPESVQEIDH
                     GQDFYAPQTLLDDQNRRILIAWMQTWGRTLPTHDQEHKWACAMTLPRILRLEDGKLRQ
                     FPVKKGQYQIQIDKDCHYHLGNDIDYLEFGYDSNAQQVYIDRSHLIQKILGEEEQDTS
                     RRYVDIEAKELEVVLDKNSIEIFVNQGEASLTATYYLTVPAELSRID"
     gene            complement(15548..16393)
                     /locus_tag="VYN69_000926"
     CDS             complement(15548..16393)
                     /locus_tag="VYN69_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1533220.1"
                     /translation="MKRFKPTTILEYILLTLLAGLFLFPMVWMVVSAMKPEADVYKNL
                     TSWQAFLPSLNPADWFKPYQEVFERFSIFTYLGNSIFYAGTFAIGSIIVNALAGFAFA
                     KVNFSGKKILFGFLLALLIIPVETVLIPQFTIINSLGLVNNRLAVIIPGLASVFNIYL
                     FRNFFIAIPEEILESAKMDGASIVRIFFRIMLPMSKPAVATVGVLSFISSWNDYIWPL
                     MVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTISTIPLILVYVVAQKYIVQGLGGS
                     GTGIK"
     gene            complement(16395..17297)
                     /locus_tag="VYN69_000927"
     CDS             complement(16395..17297)
                     /locus_tag="VYN69_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1533221.1"
                     /translation="MSKETVVDTKRREKIKDNILGYSFLAPALILLGIFLVIPVGMVI
                     YYAFTDYYLLTPDERKFVGLENFIRLTKDPIFLKSFLNTLKFVVWIIPVQLGAALGMA
                     LIVNKKRKGNMFFKVAFFAPVVMSLVVISILWLYLLNPNSGLLNAILNKVGIASQPFL
                     TSPKQAMYAIVFVSAWQGAGYQMLLFLGGMQNIPQDVYEAAELDGFSKWAQFRYITMP
                     LLKPTALFVLLTTLISAFKLIVQPMVMTQGGPLNSTITMVYYIYQQGFTDRLVGYSSS
                     IALVFTTLIGMISLVQRRVLKEDD"
     gene            complement(17393..18703)
                     /locus_tag="VYN69_000928"
     CDS             complement(17393..18703)
                     /locus_tag="VYN69_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEV1533222.1"
                     /translation="MRKGTMLSVVAGLSLAILAGCSGGTNSKQASSSNDIKVWVQFSD
                     ETNEGKAWQKVVDNFNQSGKSKYKVVTEYIPRSGSGGGYEDKVNAAITTNSLPDVITL
                     DGPNTAAYAKSKVITPLDDYLKDNNMDDVLDSIKQQGTYNGKFYAFGFSESNVGVYYN
                     KKMFKEAGIAESELPTLEKPWTWDEFNTIAKKLKDHYNKPAIDFRINSNDEMLPYAYM
                     PLIWSNNGSVVNEDGTKAEGYFNSKESVEAVQFIQNLVKEGYTTVSPVEKGFETGEYP
                     MLLSGSWTIADMNTNYKDIDFGILPYPVSSKTKKLVSPSGSWQLAVTTKSDKKEAASE
                     FVKFATNTESSEIISLGNSVLPIRKSTIENIKDKVSEPMRFLMEQNSKTAHARPVVVA
                     YPQVSRAFQQAMQDISYYEENPNVQKVLDTRTKEMQTAIDQSLK"
     gene            complement(18831..19826)
                     /locus_tag="VYN69_000929"
     CDS             complement(18831..19826)
                     /locus_tag="VYN69_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1533223.1"
                     /translation="MVEQRKSITMKDVALEAGVSVGTVSRVINKEKGIKEVTLRKVQR
                     AIKTLDYIPDNYARGMKKNRTETIALIIPSIWHPFFSEFAYHVEKELLKYNYKLFICN
                     ADSDSQNEIEYIEMLKQNKVDGIIGITYSDIDKYILSDLPFVSIDRHFSENVCYVTSA
                     NYKGGQTAAEELVRRDNKHLAFIGSINMYENETILRSKGFREKAKELGVDVYNFEMKE
                     PLINPEDQIRIFLQSHKEIDGIFTINDMMALRVISVMKELGKIAPDDYQIIGFDGLKT
                     SVDQSYLVSTIVQDIGMMAKESVAMILKMIKREEILEKRIVIPTYFWGDKTTKKI"
BASE COUNT         6161 a         4456 c         3500 g         6128 t
ORIGIN      
        1 cttgaggtat ttttgctaaa aaaaaaaagc attttactat tttatatata tatatattat
       61 aagataagtt ttttataaag acacgcaaat gagtataaat cagctaattt ttggtttaca
      121 ttcctatttt tatgcataat tattctgtca ttctctaatt tgtgaatcaa tatatctgat
      181 tagaaaacta ccattgagtt tttgctccac ctcattagtc cgctcaaaac gccgtccgct
      241 aatcttgtca tagactttct cccctctagg agcccctgca taaggacgat agaggtagcg
      301 gttgattccc gtttcgtcaa tatatacgat tggtaaatct tttaggttat ccaaaatatc
      361 aagaaactca gaaactttct caggcttttg ttccctaaaa ctggtcgtct tttttaaaat
      421 gaccttaatc tgctttaaaa ctgcccatac ggagggcaaa gcacaatcaa aacgggccgc
      481 aatttcccgt aaaaaagcgt ctggatgagc ctctacaaag gctttcaatt cttctaaagg
      541 gatcttacgc tttttgacga ctcgcttttt ccgctctaag ttcccttgtt tacgtctttc
      601 ttttcccacg tgaagagagt tctggcgccg acatcaaaaa ctttggccgc ctcgacatgt
      661 ctgtgccctc ctttaatgga atctaatgct ccttgtttaa aatcagtact atatgcatag
      721 ctttattaga ccatgcttat tttaaactaa gcaactatag atagagaaat gaaataaaat
      781 ctgaattcga atgtgataag gagttagaaa ttgaaacaat aggattcccc cattattcac
      841 acaaaataaa accactaaaa aacagagctt gccagcattc ttgaaaagta gtataattat
      901 tgcatgcgca atcatcttgt gacgcagaga ctgcccgtta atggactttc ttctatttta
      961 tgactctaaa aagaaaggag acactatgac ctatttggaa aaatggtttg acttcaatcg
     1021 gcgtcagaaa gaaattgaaa gtctcttgga agagaccatt gcccaacaga gcgagcaaag
     1081 tctgaccttg aaagagttct acctgctcta ctatctggac ttggctgaag aaaaatctct
     1141 acgccagatt gacctgccag ataagcttca tctgagcccg agcgcagttt ctaggatggt
     1201 ggctcgcttg gaagctaaaa attgcggtct actcagtcgc atgtgttgcc atcaagatag
     1261 acgctctagt tttatctgcc tgacaaatga tgggcaaaag acactggcct cgctacaaaa
     1321 gactgtcgaa gaaagcttgg aagcaggttt ggatttcttg atttaagatg attttaaaaa
     1381 aagttgcgtg cgcaatcatt tttcttgaca ttctctttta taaggagtaa aataaagtta
     1441 gcattaaaca aaggagtttt aaacatgatt gaaattacct atatagatgc cagcaagaac
     1501 gaaagaactg tgactttcga gtcttatgaa gactttgaac gttcgcaaca agcttgcctt
     1561 atcggcgtcg cagactacta ccctgtccaa aaattaactt acaagggtca taatttggac
     1621 taccatggga cttatggaga tatcttcttc tatctcatga aacaagattt aagccaatat
     1681 aactaaaaaa aggagaaata caatggcaaa agcaattaca gatgcaacat tcgaacaaga
     1741 aacaaaagac ggtttggtct tagtagactt ctgggcaact tggtgtggtc catgtcgtat
     1801 gcaaggtcca atcttggaca aattgtctga agaactttca gaagatgtct tgaaaatcgt
     1861 taaaatggac gttgatgaaa atccaaacac agctcgtgct tttggaatca tgtctattcc
     1921 aactcttctc ttcaaaaaag acggccaagt tgtcaaacaa gttgcaggtg ttcacacagc
     1981 agaacaaatc aaggccatca ttgctgaatt gagctaatca aacgagagac caagtacttt
     2041 ctttggtctc ttttttcttg ccctttgcca tttttcaaaa aatatgctag actatagcag
     2101 attgaaacta gaatagtaca cctctacttc taaaacattg ttagaaatcg atttgactgt
     2161 cctgatcgat ttgtcctgtt cttatttcat tttactatat aagtagaata ttacgatgtt
     2221 tgggatatgc catcgctaaa aaaaacctcg atttggtatt ttttagctcc ctaatgagag
     2281 ctttttgcgt gctctgaaca tttcccattt tggaaggagt actatgaaac gtcaatcagc
     2341 cttggtcgtc tttagtggcg gtcaagattc aacaacctgc cttttctgga ccatgcaaca
     2401 ctatgaaaca gtcgaagctg tcacctttgc ctacggccaa cgtcatcacc tcgaaattca
     2461 aattgctaga gaaatcgcta aggaacaggg cattcgtcac catatcctcg atatgtctct
     2521 gctgggacaa atcactgctc aaccagactt tgcgacgatt catatttcct acattcctga
     2581 caagctctgt gtcgagtcaa aatccctcaa actatatcta tttagctacc gaaatcacgg
     2641 agatttccac gaaaactgta tcaacaccat cgggaaagac ttggtcaact tgctagaccc
     2701 tcgctattta gaagtctggg gaaaattcac tccgcgcggt ggcatttcaa tcgaccccta
     2761 ctacaactac ggtaagcaag gaactaagta tgagggcttg gcagaacaac gcctcttcca
     2821 acacgacctt tatccagaga aaattgacaa ccgctaaact catactcaat gaaaatcaaa
     2881 gagcaaacta ggaagttagc cacaggttgc tcaaagcagt gttttgaagt tgtagatgaa
     2941 actgacgaag tcagcttaaa acactgtttt gaggttgcag atagaactga cgaagtcagc
     3001 ttaaaacact gttttgaggt tgtggataga actgacgaag tcagatcaaa acactgtttt
     3061 gaggttgtgg atagaactga cgaagtcagc ttaaaacact gttttgaggt tgcagataga
     3121 actgacgaag tcagtaacca tacctacggc aaggtgaagc tgacgtggtt tgaagagatt
     3181 ttcgaagagt atcatacgaa aaagccctgt tcctcaagat gaggagcaag gctttttgag
     3241 tttcaattat tcttctgttc caaggaaatt ttttgcaaca agggctgcaa gaactccacc
     3301 agcgattggt gcaaggatga aaatccaaac ttgttgaagg gctgcgccgc ctaccaagac
     3361 agctggtgcc aagctacgag ctgggtttac tgaaagtcca gtaatcttca atccgacaag
     3421 aatcatcgcc atcaatgaca aaccgattac caaaccagca atcgcgccat tgcccttgct
     3481 ttctgaagtc acagtcatga taaccaagac aaacaagaaa gttgcgataa cttcaaacaa
     3541 gaaaccacca aagacagtga caccgtttgc caaggcattt tcaccaagac tagcagttga
     3601 catacctgaa ttacccaaga ggaagaagac tgcgccagaa gcgatgaaag ctccaacaac
     3661 ctgaccaagg atgtagttta caagttctga agatgacaaa cgtttgttta caaacatagc
     3721 aatcgaaaca gccgggttca agtgagcacc tgaaacagtt ccgattgagt aggctgccac
     3781 cacgattgcc aaaccaaagg caaaggcgat tccaaggtga ccaaggccat caagaccatt
     3841 tccaaaaaca acagctcctg tcccaacgaa cacaagcatg aacgtaccga ttaactcagc
     3901 aacaaatttt ttcattttct ttctcctttt ttcaaaaact agatactagt ctatcaaaag
     3961 taggaaaggg tttcaagaaa attgattgga aattttttga aaatcataga actattagct
     4021 aatctctagt attgaaaaga ctggatagct tctttcaggt catcttgtaa actatttctc
     4081 tggtcaagtt ggacatagac ttccaccaga caggatctaa agttggaaaa tttgtaaaaa
     4141 tcctcccttt cttctatcgg aaaatcaaca gtttttatcc aagaagctac ttgttcttgc
     4201 tccaacttcc cttgtaaaat aggttcatag atcactcttg ctaaacgcca atcctcatca
     4261 tctgtaaagc gaatcgacat tcttttaaat agttggccaa gtatgtcaaa tacttcatga
     4321 attctgttta taggaaagtc tggatgacaa accacctctg tcagtaaatc ggctccatgt
     4381 gcaaaagcat gaacccaacc atactgactt gagaaatctg ttgtatcctt ttcttttgaa
     4441 agatagtgca agccttgatt tgaaaggtca tcacgaattt ctggagaagg atttcccaaa
     4501 tgatcaaaca accactggat ttcttcctgg ttataatttg gtttttcttc tgctattttt
     4561 cttagtaaat cttgatacat ggtcaatacc tctacatttc tagcaactgt tcaaaaaggc
     4621 agtcttaaat gactcaatat tgaattctca attaaataca atctgatata aaatgacgta
     4681 aataactatc aataccagtt ctacagtaag ttcaaattta acatcacgac cttcaacgac
     4741 atttttgaaa atagctacaa ctaagacaaa tagaatgacg cttaacaagc ccataaacat
     4801 cattctagaa aatttttcta ttccccttac tcccccaact cagcactgta ggcgataatc
     4861 tggtcaactg tgtcagacaa gaattggatg gtatcacgga gtggtttgtc tgttgaaata
     4921 tcagcaccga taatcatggc tgactcaagt ggtgtcttgc taccacctga tttgaggaga
     4981 ttgagccagt cttcagctcc agtttctgag tttttaagat gaaggtaacc agctgtcgag
     5041 atgacaagac cagcagagta agtgtaacta tacaagccca tatagtagtg agcttggcgc
     5101 atccaagtca aagctgcatc gtcgtcgatt tcaatggcat cgccccaaaa atctgtcaaa
     5161 acttccttca taatgctgtt gagcttgctt gctccaaagg tctccccttc ttcaatcaat
     5221 gtatacacct tacgctggaa ggcggcttcc aagaggtggg tgatgaaatt atggaagtag
     5281 gtgtctgtca agcgatgagc cagagcgaag cgtttttgac gtgggtcatt agactggttc
     5341 tctaagtaat cactgagtag caattcattg aaggttgacg gtgcttcaac ataataggtc
     5401 gacatgtggg cgttgaaata gctttggtga ttgtctgaaa agatgaattg accagaatgc
     5461 ccgatttcat gaatcaaggt atagacatcg ctcaaacggc ctgtccagct catgagtaca
     5521 taagggtgta cgcgatatgg gtccgccgca taaccaccgg aatccttgcc actgttagca
     5581 gcaaagtcca cccagcgctc ttcttggtaa cgagcaactt cctgacaata ttcttgcccc
     5641 aaaggttcta ccgacttcat gaccaaatca taggcatcgt caatagtcac ttcaggattc
     5701 agggcgctgt ccaagtccaa tttccagtct gcaaaggtca tcttttcaag accatttacc
     5761 ttggcaacat gcttgaggta tctctgagcg actggtgcaa agtccttcat gatgaggtca
     5821 atctggcggt caaacatgac acggtccact tcttgttcag ctagaagata atcaaataca
     5881 gagtcgtatc ccttcatatc agccaagagt ttttcagact tgacctgagc cagataggct
     5941 gctgcagccg tattttggtg cttacgaagt ccctctgaga aggaacggaa ggatttctca
     6001 cgaacctcag catcctcgtg attttggtag aaattctcat aggtgacaaa gctgttttta
     6061 taggtcttgc catgggcttc aaagtcagcc atttcaaaat ccccagctcg catcttagta
     6121 taaatgtcct gcggactgta gaaaacttca ccgagattgg tcaaggcctt ctccacatca
     6181 gcccctaagt agtgggcttt tttgattttg gcctgacgaa tagctgccgt caagtgaggc
     6241 aattcaccca aacggtccaa gacttcctcg tctgctacca ccaaggcatc gtcaaagaag
     6301 gtcaaggcta cgctggcatc tgtttcaaat tccatcccag cttgggcaat attggcaaat
     6361 tcgtcattgc tatagtccgt cgtctgaggc ataaaaccat agttgccaat atggctcatc
     6421 tgaatgtaga tctgttccaa ttccgcaaag gccttctcga aatcctcaaa aatgtgaaga
     6481 ttgcccttgt aatcacggct aaactggttg atgtcttcgc gagctttctc gattgcacgc
     6541 aagaaatcct cacggtcttg gtatagggct gttaaatccc aaagttcctt ctctggaaat
     6601 tctgaacggt gtttttgttc cattttcttc ctcttatttc tctaattcta ctaaaacact
     6661 aagggctgat aaggcgtaaa gcggtgctgt ttctgctcgc aaaatacgag gacctaggcc
     6721 tgccaaaacg gctcccttag cttcaaaact ttcgatttct gcaggtgaga gaccgccttc
     6781 tggaccaaag ataaagagca gtttggctcc tgtttcaaga ccagtgactg cttgcagaag
     6841 cgcagcggct tctccttctt tagctgattc ttcataggct actatgatag agtcaaactg
     6901 gtccagctga gctagaaaat ctgctttttt ctcgaaaagt ttaatacttg gtacaatatt
     6961 acgcttgctt tgctcggctg ctccaagggc aattttttct agtttttcaa cttttttacc
     7021 caatttcttg ccatcccact tggcaactga ccagtctgca ggaaaggccc agatttggct
     7081 agcacccagt tcggttactt tttgagcgat gaactccagc ttgtctccct tgggaaatcc
     7141 agatgcgatg gtcacttgga ctggtagttc cacattgtca tttaattctt ggaccaactc
     7201 aaactgacga ttttccatat ccagcacgcg cgccaagcgc ttgatgccat catcaaagac
     7261 taaggtaacc tcatcctctt ctttcaagcg cataacctga aacatatgct tactggtttc
     7321 cttgtcctcg atagtgacag gagagatagc actgcctttt acaaaatact gctgcatgct
     7381 agcctccaat cacaccagag atatccttgg ttttcttaaa gacacaggta ttccattccc
     7441 cttgaaccat gtgagtttcg aggaaaaatc cagctgattc agccgactcg cgcaccatgt
     7501 cccacttatc cttgataatg ccactcatga tcaggtagcc ttcgtcctta accaaacgat
     7561 aggcatcgtc tattagatga atgaggatat ccgccaagat attagctaca atcacatctg
     7621 cctcaatctc aacaccctta agcaagtctc cagcagcaac gtggatattt tccattccag
     7681 ggttaagttc aatattttcc tgagcaacac gaaccgccac atcatccagg tcataggcga
     7741 agatttcctt ggcaccaagt agcgagctag caatggagag aacccctgaa ccagtcccca
     7801 catctagcac tgtctcgcca ccacgaagaa cctgttccaa ggcaaagagg ctcatcttgg
     7861 tagttggatg ggtcccagta ccaaaagcca tcccaggatc tagcttgata attttttctc
     7921 ccgcagtcgc ctcatagtct gtccaagagg gaacgatggt caaatcatga gtgatacgag
     7981 ctggctcata gtatttcttc cagttgtctg cccagtcttc ctcagccaag gcagtcgtcc
     8041 ccatcttgac ttcacccaaa tccataaaat ccgtcaattc tgctaagcga gcctgcaagt
     8101 ccgcctcaac cactgtcaca tccaccgtgt cagggtagta ggctgtcact acgatttctt
     8161 cttgctgctc cacctctggg aaaatctcgc caaaacgatc cacatttccc atatagtcca
     8221 tgctgtcttc aatcgcgact ccttgcgctc ccagctcaat caagagattg gaaaccaact
     8281 cctctccctc acgcttcact gtaactttta actcttgcca tgtttccatt attaagatac
     8341 caagcccgta aaacacaaaa ccaaaatagg aaattctctg aagacgcttg tgtctaagag
     8401 aagtttatct ttttggcaca gtgtttaggg cgggttcagt ttagaaattt aactgaacca
     8461 tcctttctaa tcacttactt ttaaataatc ttttaatctc tcttgcaact gaggcacaac
     8521 ttgactggaa ctaagaaatt cctcaacatt catcagctga tagccctgtc cttcatctcc
     8581 aaatacgata ctgtcaaact ggttctgtgt caactgacca actagaaaaa cggactgttt
     8641 gtctgcaaaa agcatacttg ggtaaacctt actccaaagc agacaatctt cagtcagatg
     8701 aatgcccagt tcttcataaa cttcacgcgc tacacactca aaaggacttt catcaccttc
     8761 acgtccacca cctggcagtt cccacatatt ggcccaggga atacttgcct tatcatcacg
     8821 taagatagtc aagagcttat ccccacaaaa caaagcaatc ttacagcctg ggaaaccaga
     8881 aatttctagt tccatcttca gttccttcta acatttcctt ttccagctcg gctaacaagt
     8941 tttcataata gcgtttctcg tccctcaaca ttcgactact atccattttc tgtctagcaa
     9001 tcttgagagc cttacgagtt cgatctacat ctttcttcac ctttaattga taccaggctt
     9061 gtatcacttg aagattggac agtttgagag acagaaacga tttgacctgt cgaatactag
     9121 catattgctc cgcttgctca aaatctcctt ccaacaaggc gatatgaagc agggatagtt
     9181 gggcaactgt ctgcatcatc ggagtagttg ccctctcaag taatgcttga aactgctgtt
     9241 tagctacttc ttccttccct tccaaaatgg aaacttcacc ttgcatacct aatacaccat
     9301 ccgcaaaact ccctcgtgca tcctcaggaa ctgcttgaac aaagtctttc aaatcatatt
     9361 cttgaggagc cagcaaagtt tgagcagaat gtctcaatac caggtaggcg tatttggtat
     9421 tttcagggtg ttgtagtaat tcccaaattt ttgctccatc ggtgatgtcg actggcaaaa
     9481 tgttatttag gaagaaagat aaattaagaa aaatccaagt ccctgcaaaa taccagcttc
     9541 ttgtcaaaaa tccaaacact atcgccaata atatcaagcc gagatgaacc atcaagcctc
     9601 ctgaaagcat caggatgatt ctttgatcgc tttcatcctc ttttaaacca atgtattgag
     9661 caccaacatt tttcagaatg gctgttctac taagatgaaa cctgcctgac tttttggtca
     9721 aaataaaatg tcctaatcca aaagccacca gccgatagcc tgtcaagtag ccacaaaaag
     9781 catgacccag ctcatgaaga ataaaaatta aatacatgct tagaagagcg aaggcataac
     9841 caaaagtaaa ggctaaaact gcggaatacc ccaactctgc aaatgcgatt gttccacaag
     9901 caaaagctag cataataaag acaacagcta gcacataaac caaataagtc ccaattttct
     9961 tcataacacc tccaaccaac tcctagtatc tcggataagg ataaaattct ccctcttcca
    10021 agccaacttt tccttcttca aagacttctt ggttccattc catgataaat tcctctgctt
    10081 ctgggtcttc caaaaagtcc atgaggacat ctagcccaac ctcagcagta tctttaagga
    10141 aaagcgaaaa ataagctaaa aattcacggg aaaatccttt tttaggcagg taaggaataa
    10201 cagtcaaata gtcttcctca ttgactgttg acttggcagg attgtagaaa aggaccgctt
    10261 cctcaaaaag aatgtcatct gatgaaacct ctccgtcttc atccaccatc tccacacctg
    10321 cagcattttg cgcttccaat agaaaactca cttctaccgc atggttgcgt ttgtcccagc
    10381 taatctcaaa gtcaaaggga aagttcttgt ccaactcttc ctctaaaaca tctaaaaatc
    10441 cgtatgttgc cattttgtcc tctttctatg cgactcttta atcgccccga ttgctcggaa
    10501 atatgctaaa atagatacta ccatcttacc acaaaattat tttatgtcct aattatacca
    10561 tattacctca tttaaaccct tggtatcagt gattttctta aaagtctgat ttcttcattt
    10621 ctcataaaaa tcaatataaa aagcccgcga aagggctaat aaatctataa aatcaatagg
    10681 cgagtaacta gcacaagtgg acgtgctttt ttattgacta ttaccacgat accacgctta
    10741 atcttaggct tgaactttct tatctgcaat agcgtctgtc aaagtctgag aaaagttaag
    10801 tcccatttct cgtcccaact tatctgccca ttttggtatg gtcaaagtct ttttaatggg
    10861 ttcctgactt cctaggtatt ctgatacatc aacagatacc atagaaataa aagatttatc
    10921 aaggtcatag gttgacacga aatcttcatc atctttaaaa ggatcattat caattaaaga
    10981 caagctattg atatctgatg gctgaggtaa ctctccatca ctctctatca aatctgcaac
    11041 agttatccct agccactccg accccatagc caaagcctca gaaatcccct ctccttgtgt
    11101 agctgagtat tcaaaatctg ggaaatggac aaaataagtc gcttctgctc cgtctgtgtc
    11161 gtcataataa aataaagctg gatacgtaac taacatttca ctacctccat atcaaaaagc
    11221 aggggctgaa ttttacaacc cagcttgctt tcttatccct ctttcagtgt acttattcag
    11281 ctcaccatga aggattgtga taggtctttc cccttgcttt tccattttaa tatgggagcc
    11341 tttaccgcct ctagtcttta tccaaccatg ggccgtaagg agtttaacca tctctttttg
    11401 tgtcataggc atagcgcttt tacctcctga caacaccatt ataacacgtg ttacacgtat
    11461 tgtaaaggag tgatacttat tattctatta tacataaaag cccctagatg tggttctaag
    11521 ggaagccaat ttattcatac ctatttttct aatgagtagt aaaaactgct tctttatcga
    11581 gcaattcatc atctgtatag tcaattgtaa aagtatctcg atctaagaca gattgaggcg
    11641 gagttgaatg aatcatagga ccactgcgta ctctatattt tttatctcca atttttacaa
    11701 actgatactc ttcgaaaatc tcttcaaacc acgtcaacgt cgccttaccg tactcaagta
    11761 cagcctgcgg ctagtttcct agtttgctct ttgattttca ttgagtatga ttaactctca
    11821 agtcttcgaa atcaggattt tcaacagtta ttacaaggag gcgatttact acttcaaaaa
    11881 catcaattat tctatttttc atattttttc aacccattat tagaatgaac ttcttggtaa
    11941 gcaaaatcaa gtttagattt aatgttttcg tacaaatcta aaatctcttt tggagtatct
    12001 tcccggaaga aaagttttct tttccctgaa ataacttgat cactaagaat ccaatgacga
    12061 atttgttttg taaaaatcaa aatttcctga cttggtccat catttccatt gcttatcacc
    12121 tctcttttca ttatagttca tacaatgaca tttagcaata ttatttctca agtcagcact
    12181 tccacttctt taggctcaac tatcctattt tgagctttaa ggaaaatcaa atctctcatg
    12241 ctgatacctc tcctcattaa attaaatagt aaaaaagatt ctatctcact ccctgattat
    12301 tacaaaacca ttgaaatatc acaactaata ggctagaatg gacatagtaa gatatagtag
    12361 atgagtcatt ctactcaaat ccacgttaga aaggactgct atgccagaca atctcgcgct
    12421 tcgcatgcgc cctaaaacca tcgaccaggt catcggtcag gagcatctgg tcggacctgg
    12481 aaaaatcatc cgccgcatgg tggaagccaa ccgcctgtcc tccatgattc tatatggccc
    12541 tcctggaatc ggcaaaacca gtattgcctc tgccatcgct ggaacgacca agtatgcctt
    12601 tcgaactttc aatgcgacag ttgatagtaa aaagcgactg caagaaatct cggaagaagc
    12661 taaattttct ggtggtctcg tcctattgct agacgaaatt catcgactag ataagaccaa
    12721 gcaagacttc ctcttgcctc tcttggaaag tggactggtc atcatgattg gagcaacgac
    12781 tgaaaatcct ttcttttccg tcactcctgc cattcgtagc cgtgttcaaa tttttgagtt
    12841 ggaacctctg tctaaccaag acgtcaaaga ggccctgcag atagctctaa gtaaccctga
    12901 acgtggtttt aattttccaa tagaactaga tgaggatgcg ctggatttca tcgcaacctc
    12961 tacaaacgga gaccttcgct ctgcctttaa ctcactggac ttggctgttc tctctacccc
    13021 tgagaatgac gagggcattc gccacatcac cttagacatc atggaaaata gtcttcagag
    13081 aagctacatc actatggaca aggatggaga cggtcactat gatgttctat ctgccctgca
    13141 aaagtctatt cgtggctcag atgtggatgc cagtctccac tatactgccc gcttgattga
    13201 ggctggggat ctgcctagtc tcgctcgtcg cttgactgtt atcgcctatg aagatatcgg
    13261 cttagccaat cctgaggccc agattcatac cgtgactgct ctggatgctg cccagaagat
    13321 tgggttccca gaagcccgca ttctcattgc caatgtcgtg attgatttgg ccctttctcc
    13381 aaaatccaac tcagcctatg tagctatgga taaggcactt gctgacctca aaacatcagg
    13441 gcacttgcct attccgcgac acctacgtga tgggcactac agtggaagca aggaactggg
    13501 gaatgcccaa gactatctct atccacacaa ctatcctgga aattgggtca agcaagacta
    13561 tctgccagaa aaaattcgta atcatcacta tttccaagca gaagatactg gtaaatatga
    13621 acgggctttg gctcaaagaa aggaagctat cgaccgtttg cgaaaaatct gaaatccttt
    13681 tcaaaaaatt gcactttcct cttgattttt tttgaaaaag tggtatcata taagtataga
    13741 aacgctgtgg tgtacgactt cacacttaag tgttgaccga ctattttttg tattattagg
    13801 gaaacaaaag tcttctaaca gcatgtaggc cgtctcacac ggaaacagct tcagttagag
    13861 cgagttgccc acctgcttaa ttgcgcgggt tcaatacaaa ccgtgaagtt tcggcaccaa
    13921 tacagctttt ttctttgcct ccttagctca gctggcagag cagcggactc ttaatccgtg
    13981 ggtcacaggt tcgatccctg tagggggcat ataaatacaa caggaaaagc cttataatat
    14041 agggcttttt ttgcttaaat aaaatttttt tcaaattctt tagaaaacta gtttaaatca
    14101 taatccaaca ccaattttac aaataccata aactaagctt atatacccaa aacacaaaaa
    14161 gtcctctttc cactatttta gaaagagaac tttttcttta ggagaaataa cttaattttt
    14221 aatcaattcg tgatagctca gctggcaccg ttaagtaata agttgcagtc aagcttgctt
    14281 caccttgatt gacaaaaatc tcgatggaat ttttatctag aacaacttcc aattctttag
    14341 cttcaatatc tacataccgt cgactagtgt cctgttcttc ttcacctaga attttttgaa
    14401 taagatggct acgatcaatg taaacttgct gcgcattact gtcataacca aattcaagat
    14461 aatctatatc atttcctaag tggtaatgac aatctttatc tatttggatt tgatattggc
    14521 cttttttaac agggaattgt cttagtttgc catcttccaa tcttagaatt ctaggtaaag
    14581 tcatggcaca tgcccatttg tgttcttggt catgggttgg aagggtacgc ccccatgtct
    14641 gcatccaagc aatcaggata cgacgatttt gatcgtccaa caatgtttga ggcgcataga
    14701 agtcttggcc atgatcaatt tcttgaactg attctgggat aaaacatttt tctccccact
    14761 ctaccttacc cgtgaacaaa agcgatgagt tgatgttatg atatgagtct ccctcacgct
    14821 gataacgcat gggtgacata ataaggcaat ctttcccatc taactcgaag taatctgggc
    14881 attcccacat aaagccttgg tgttctaccc cttttaaaaa gatggattcg aactgccatt
    14941 ctactaggtt atcggaccct agtagaacga tacagcccac attatccttg tgtttggtag
    15001 ctactacgga gtaatagcgt ccatcttttt caaagagttt tggatcacgg aaatcagcag
    15061 caatcaactc atctggcaag tctgctccag ttgcaactgg attttgggaa atcttttcaa
    15121 agtgaatccc gtcatctgaa aatgccatat tttgcacttg gcggacaccg gttttttctt
    15181 cgatatgccc agtgtacatg atccagaggc gatcatcctt gacaatggca gagcctgaga
    15241 aacaaccatt tcggtcataa tcttggtcag gagcaagtgc cactggcaag tgctcccaag
    15301 tcaccaagtt cttactttta gcatgtcccc agtgcatagg cccccaaaca ctatcatatg
    15361 gatagaattg ataaaagaga tggtattctc cacgaaaata aacaaatcca tttggatcat
    15421 tgatccaacc aatctcagct gaaaaatgtt cttcaggctt atattgagta ttaacgagat
    15481 gtttattttc tgctataaaa cgattggctt tttctactgt aaatgtttta tttatcataa
    15541 gtatatccta ttttattcca gttccggaac cacccagacc ttgaacaata tatttctgag
    15601 cgactacata gaccaaaatc aacggaatcg ttgaaatagt taatacagcc attacctgat
    15661 tgatataaac tggttgagtt gtattaatag ttgtaattgc aacctgcatt gaaaatttag
    15721 aagaatctgt caaaaccatc aatggccaga tataatcatt ccagcttgaa ataaatgata
    15781 aaacaccaac tgtagcaact gcaggtttag acattggaag cataattcta aagaaaattc
    15841 taacgatact tgctccgtcc atctttgcag actcaagaat ctcttctgga attgcaataa
    15901 agaaattcct aaataagtaa atattaaata cactcgccaa tcctggaata ataactgcaa
    15961 gacgattatt gaccaaacct aaggaattga taatggtaaa ttgtggaatt aacacagttt
    16021 ccactggaat aataagtaaa gcaagtaaga aaccaaataa aattttctta cctgaaaaat
    16081 tgacttttgc aaaagcgaaa ccggccagtg cattgacaat aatagaacca atagcaaacg
    16141 ttccagcata aaaaatgctg ttccctagat atgtaaaaat actaaagcgc tcaaatactt
    16201 cctgataagg tttaaaccaa tcagcgggat ttaaacttgg taagaaggct tgccaactag
    16261 tcaaattttt ataaacatcc gcttctggct tcatagctga aactaccatc caaaccatag
    16321 gaaaaaggaa gagtcctgcc aataaagtta acagaatata ttctaagatt gttgttggtt
    16381 taaatcgttt catcttagtc atcctccttc agaacgcgac gttgtactaa gctaatcata
    16441 ccaattaacg ttgtaaatac taaagcgatt gagctagaat atcctacaag acgatcagta
    16501 aaaccttgtt gataaatata gtacaccata gtaattgttg agtttaatgg cccaccttgc
    16561 gtcataacca ttggttgtac aatcagctta aaagcagaaa ttaaagttgt taacaataca
    16621 aacagggctg ttggttttag taggggcatc gtgatataac gaaactgagc ccattttgag
    16681 aatccatcta attcagcagc ttcatagaca tcttgtggaa tattctgcat tcctcctaaa
    16741 aataaaagca tctgataacc tgcaccctgc caagcagaga caaaaacaat tgcatacata
    16801 gcttgtttag gactagtcaa gaaaggttga gaagcaatac ctaccttatt taaaatagca
    16861 tttaataagc cactatttgg atttaaaaga taaagccaaa gaatagaaat aactaccaag
    16921 gacatgacaa ctggagcaaa aaaagccact ttaaaaaaca tatttccttt tcgcttttta
    16981 ttgacaatca aagccattcc taaggccgct cccaattgaa ctggaataat ccaaacaaca
    17041 aatttcaaag tattcaagaa gctctttaag aaaatcggat cttttgttaa tcgaataaaa
    17101 ttttcaagtc caacaaattt tctttcatct ggtgtcaaca aataataatc tgtaaaagcg
    17161 taataaatga ccattccaac tggaattact aaaaagatac ctaataaaat taaagctgga
    17221 gctaaaaaac tataacctaa tatgttatcc tttatttttt cacgtctttt tgtatccaca
    17281 acagtttctt tactcatatc catctccttt aatcatatat atcaaactag gctattaaca
    17341 agaaagggga aggtagttcc aaacattttc cttccacctt cttttttaca gattatttga
    17401 gtgactgatc aatagcagtt tgcatttctt ttgtacgagt atccaagact ttttgcacat
    17461 ttggattttc ttcataatag ctaatatctt gcattgcttg ttgaaaagct cgagaaacct
    17521 gaggatatgc tacaacaact gggcgagcat gagctgtttt tgaattttgt tccatcaaga
    17581 aacgcattgg ttcggatact ttatctttga tattttcaat tgtagattta cgaattggaa
    17641 ggaccgaatt ccccaagcta ataatttcac ttgattctgt atttgtagca aatttaacaa
    17701 attcagatgc ggcctctttt ttatcagatt ttgtcgtcac agctaattgc caactacctg
    17761 aaggggatac taattttttc gtcttactag atactgggta aggaaggata ccaaaatcaa
    17821 tatctttgta gtttgtattc atgtcagcaa tcgtccaaga accactcaaa agcattggat
    17881 actctcctgt ttcaaaacct ttttcgactg gactaacagt tgtatagcct tcttttacaa
    17941 gattttgaat gaattgaact gcttctacac tctctttgga gttgaagtat ccttctgcct
    18001 ttgttccatc ttcgtttacg acagaaccat tatttgacca aatcaacggc atataagcat
    18061 atggcaacat ttcatcgttt gaattaatac gaaaatcaat tgctggtttg ttataatgat
    18121 ctttcaattt tttagcaatc gtattaaatt cgtcccatgt ccatggcttt tctagcgttg
    18181 gaagctctga ttccgcaatt ccggcctctt tgaacatttt tttattgtaa taaacaccaa
    18241 cattcgattc agaaaatcca aatgcataga atttcccatt ataagttccc tgttgtttaa
    18301 tactgtccaa aacatcatcc atattattat cttttagata atcatctagc ggagtaatta
    18361 ctttagattt tgcataagct gccgtatttg gtccatcaag ggtaataaca tctggaagac
    18421 tattagtcgt aatagcagca ttaaccttgt cttcatatcc tccaccacta ccactacgag
    18481 gaatatactc agttacaacc ttgtacttac tttttcctga ttgattaaag ttatcaacaa
    18541 ctttttgcca agcttttcct tcatttgttt cgtcagagaa ttgtacccag actttaatat
    18601 catttgaaga actcgcttgt tttgagttag ttcctccact acaacctgca agtatagcta
    18661 acgatagccc cgcaacaaca gataacattg tacctttacg catcattttc ctcctttttg
    18721 aaacgtttca ttttttattt attttttaaa caagcttcta gcctattttt tgaaacgttt
    18781 catttgttga ttatattata acagcgcttt cattaattgt caagcgcttt ttaaattttt
    18841 tttgttgttt tatctcccca aaaatatgtg ggaattacga ttcgtttttc tagaatctct
    18901 tctcgcttaa tcattttcag tatcattgca acagattctt ttgccatcat accaatatct
    18961 tgaactattg tagacactag ataagattga tcgacagaag tttttaaacc atcaaaccca
    19021 attatctggt aatcatcagg tgctattttc cccagttctt tcataacact aataactctc
    19081 agagccatca tatcatttat tgtaaatata ccatcaattt ctttatgaga ttgcaaaaaa
    19141 attcttatct gatcttcagg atttattaaa ggttctttca tttcaaaatt atagacatct
    19201 actcctaatt ccttagcttt ttctctgaat cctttacttc tgagtatagt ctcattctca
    19261 tacatattaa ttgaaccaat aaaagcaagg tgcttattat cccttctaac cagttcctct
    19321 gctgcagttt gtcctccttt gtaatttgct gaagtaacat agcatacatt ttccgaaaaa
    19381 tgtctatcta tacttacaaa cggtaaatca gataaaatat atttgtcaat atcagaatat
    19441 gtaataccaa taattccatc cactttattt tgtttcaaca tttcgatata ttcgatttca
    19501 ttttgtgaat cgctatcagc gttacaaata aataatttat aattatattt taataactct
    19561 ttctcaacat gataggcaaa ctccgaaaaa aatggatgcc atatgctcgg aataatcaaa
    19621 gcgatagttt ctgttctatt ctttttcata cctcgagcat aattatcagg gatatagtcc
    19681 aatgttttta tagccctttg aactttcctt aaagttactt ctttaatgcc tttttcttta
    19741 ttaattacac gtgaaacagt tccaacacta actcctgctt ctaaagcaac atctttcatg
    19801 gtaattgatt ttctttgttc taccatatta tcacctcctt tcaatatata gtatcatgca
    19861 aatgcttttt aagcaactat ttctcaatca tttttggcca gatcatttat cccatcatga
    19921 ataaaatcac tccaattagc ttttgaaaat acttcaattt tcatgtgtaa acatctacat
    19981 aaaacaggaa aagccttggt tttatgactt ttttcgtatc ttctacaaaa aaagcaagag
    20041 ttttagaggg ctataaatct agatgtacat tttgcttaaa tgattgaagg tcttttctta
    20101 acaaaaacac cccaaaaatt agattttttc tgtctaactt ttgaggtaca gttcaaacgc
    20161 gaaatagcgt ttttttgtta tttttggtta ctcatctaat cgaataaaca tcatggcatt
    20221 taacaaatat atgagtgaga ccgtg
//
LOCUS       DAVLVP010000026        20188 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.026, whole genome shotgun sequence.
ACCESSION   DAVLVP010000026 DAVLVP010000000
VERSION     DAVLVP010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20188
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.026"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            151..333
                     /locus_tag="VYN69_000930"
     CDS             151..333
                     /locus_tag="VYN69_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1533224.1"
                     /translation="MDLKFEGVDLEYKKAKNNLPESFWETYSAFANTNGGKIILGIDE
                     KNIDTYQRVNRLPAKL"
     gene            278..1127
                     /locus_tag="VYN69_000931"
     CDS             join(278..691,693..1127)
                     /locus_tag="VYN69_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1533225.1"
                     /translation="MKKTLTPTKESIDSLRNYEASKQLTDARFKRLVGVQRTTFEEML
                     AVLKTTYQLKHAKGGRKPKLSLEDLLMATLQYVREYRTYEEIAADFGIHESNLIRRSQ
                     WVEATLVQSGVTISRTPLSSEDTVMIDATEVKINRPKKELANDSGKKKCHAMKAQAIV
                     TSQGRIVSLDITVNYCHDMKLFKMSRRNIGQAGKILADSGYQGPMKIYPQAQTPRKSS
                     KLKPLIAEDKAYNHALSKERSKVENIFAKVKTFKIFSTTYRNHRKRFGLRMNLIAGII
                     NHELGF"
     gene            complement(1199..1561)
                     /locus_tag="VYN69_000932"
     CDS             complement(1199..1561)
                     /locus_tag="VYN69_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533226.1"
                     /translation="MPKTKTLAELADVILWSFDFANDHAHTFFMDNVEWSHADSYFLS
                     FVSDDVEERYTENVYLDSLSVKQKFKFIFDFGDEWRFECQVLREIETEDEEAYLVRSV
                     GTSPEQYPDYDGFDYEEW"
     gene            complement(1939..3819)
                     /locus_tag="VYN69_000933"
     CDS             complement(1939..3819)
                     /locus_tag="VYN69_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HEV1533227.1"
                     /translation="MVRFTGLSPKQTQAIEVLKGHISLPDVEVAVTQSDQASISIQGE
                     EGHYQLTYRKPHQLYRALSLLVTVLAEADKVEIEEQAAYEDLAYMVDCSRNAVLNVAS
                     AKQMIEVLALMGYSTFELYMEDTYQIEGQPYFGYFRGAYSAEELQEIEAYAQQFDMTF
                     VPCIQTLAHLSAFVKWGVKEVQELRDVENILLIGEEKVYDLIDGMFATLSKLKTRKVN
                     IGMDEAHLVGLGRYLILNGVVDRSLLMCQHLERVLDIADKYGFHCQMWSDMFFKLMSA
                     DGQYDRDVEIPEETRVYLDRLKDRVTLVYWDYYQDSEEKYNRNFRNHHKISHDLAFAG
                     GAWKWIGFTPHNHFSRLVAIEANKACRANQIKEVIVTGWGDNGGETAQFSILPSLQIW
                     AELSYRNDLDGLSAHFKTNTGLTVEDFMQIDLANLLPDLPGNLSGINPNRYVFYQDIL
                     CPILDQHMTPEQDKPHFAQAAETLANIKEKAGNYAYLFETQAQLNAILSSKVDVGRRI
                     RQAYQADDKESLQQIARQELPELRSQIEDFHALFSHQWLKENKVFGLDTVDIRMGGLL
                     QRIKRAESRIEFYLAGQLDRIDELEVEILPFTDFYADKDFAATTANQWHTIATASTIY
                     TT"
     gene            complement(3813..4682)
                     /locus_tag="VYN69_000934"
     CDS             complement(3813..4682)
                     /locus_tag="VYN69_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEV1533228.1"
                     /translation="MTIATIDIGGTGIKFASLTPDGKILDKTSISTPENLEDLLTWLD
                     QRLSEQDYSGIAMSVPGAVNQETGVIDGFSAVPYIHGFSWYEALSSYQLPVHLENDAN
                     CVGLSELLAHPELENAACVVIGTGIGGAMIINGRLHRGRHGLGGEFGYMTTLAPAEKL
                     NNWSQLASTGNMVRYVIEKSGHTDWDGRKIYQEAAAGNALCQEAIERMNRNLTQGLLN
                     IQYLIDPDVISLGGSISQNPDFIQGVKKAVEDFVDAYEEYTVAPVIQACTYHADANLY
                     GALVNWLQEEKQW"
     gene            complement(4766..7411)
                     /locus_tag="VYN69_000935"
     CDS             complement(4766..7411)
                     /locus_tag="VYN69_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000432759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEV1533229.1"
                     /translation="MENVVVHIISHSHWDREWYLPFESHRMQLVELFDNLFDLFENDP
                     EFKSFHLDGQTIVLDDYLQIRPENRDKVQRYIDEGKLKIGPFYILQDDYLISSEANVR
                     NTLIGQQEAAKWGKSTQIGYFPDTFGNMGQAPQILQKSGIHVATFGRGVKPIGFDNQV
                     LEDEQFTSQFSEMYWQGVDGSRVLGILFANWYSNGNEIPVDKDEALTFWKQKLSDVRA
                     YASTNQWLMMNGCDHQPVQKNLSEAIRVANELFPDVIFVHSSFDEYVQAVEGALPEHL
                     STVTGELTSQETDGWYTLANTSSSRIYLKQAFQENSNLLEQVVEPLTIITGGHNHKDQ
                     LTYAWKTLLQNAPHDSICGCSVDEVHREMETRFAKVNQVGNFVKSNLLNEWKGKIATD
                     KAQSDYLFTVINTGLHDKVDTVSTVIDVATCDFKELHPTEGYKKMAALILPSYRVEDL
                     DGRPVEATIEDLGANFEYDLPKDKFRQARIARQVRVTIPVHLAPLSWTTFQLLEGEQE
                     HREGIYQNGVIDTPFVTVSMDDNITVYDKTTHEAYEDFIRFEDRGDIGNEYIYFQPKG
                     TEPIFAELKGHEVLENTACYAKILLKHELTVPVSADEKLEEEQQGIIEFMKREAGRSE
                     ELTNIPLETELTVFVDNPQIRFKTRFTNTAKDHRIRLLVKTHNTRPSNDSESIYEVVT
                     RPNKPAASWENPENPQHQQAFVSLYDDEKGVTVSNKGLNEYEILGDNTIAVTILRASG
                     ELGDWGYFPTPEAQCLREFEVEFAIECHQAQERFSAYRRAKALQTPFTSLQLARQEGS
                     VVATGSLLSHSVLSIPQVCPTAFKVAENEEGYVLRYYNMSQENVRISEHQQTILDLLE
                     RPYPVHSGLLAPQEIRTEFIKKEEI"
     gene            complement(7502..8782)
                     /locus_tag="VYN69_000936"
     CDS             complement(7502..8782)
                     /locus_tag="VYN69_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEV1533230.1"
                     /translation="MVYSKEIVREWLDEVAERAKDYPEWVDVFERCYTDTLDNTVEIL
                     EDGSTFVLTGDIPAMWLRDSTAQLRPYLHVAKRDALLRQTIAGLVKRQMTLVLKDPYA
                     NSFNIEENWKGHHETDHTDLNGWIWERKYEVDSLCYPLQLAYLLWKETGETSQFDEIF
                     VAATKEILHLWTVEQDHKNSPYRFVRDTDRKEDTLVNDGFGPDFAVTGMTWSAFRPSD
                     DCCQYSYLIPSNMFAVVVLGYVQEIFAALNLADSQSVIADAKRLQDEIQEGIKNYAYT
                     TNSKGEKIYAFEVDGLGNASIMDDPNVPSLLAAPYLGYCSVDDEVYQATRRTILSSEN
                     PYFYQGEYASGLGSSHTFYRYIWPIALSIQGLTTRDKAEKKFLLDQLVACDGGTGVMH
                     ESFHVDDPTLYSREWFSWANMMFCELVLDYLDIR"
     gene            8954..11038
                     /locus_tag="VYN69_000937"
     CDS             8954..11038
                     /locus_tag="VYN69_000937"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEV1533231.1"
                     /translation="MKPLLETIDTRFGTTNKHAFSRGNTLPYTGVPFGMNYFVPQTSD
                     QDGSWFFDPHLPIFQGIRLTHQPSPWIGDYSWLLLTPVTSQLGGDSLFHRQSSYDRDK
                     ASFQPHYLKIFSLRYQIETQLTPTCYGASIRLKQKQGKALSLYLHATDELTVEQVDKR
                     TLALRQEGKTETNKNSLTMFTALQMNTDILAISQEGGDWRIDLASSQTEMQLATSFIS
                     PSQALLNLPQEDFDSCKSSAQADWENLLHRFDIIEIGEADRTFFDHCLYRLFLFPQTF
                     YEINESGQAIHMDLATGTVKPGVLFSNNGFWDTFRTTFPLFALIIPEHYQRFLEGFLN
                     SYRDTGFLPKWLAPDERGMMPGTLLDGIIADSACKDMAPDLEGELFQAMLETASKADP
                     LGINGRHGLAQYQELGYLSTDHHESVSHTLDYAYSDFCIANCAKKLKNIEIAETYKAA
                     SQNYRQLFDAETGYMRARDNQGNFHPDFSPYSWGRDYAECSAIQATLGVLHDIPGLIQ
                     LMGGKETFSNYLLKACQDAPLFETTGYGYEIHEMSEMATAPFGQIAISNQPSFHIPYL
                     FRYSDYPDYTALLIKTLRQKAFHPSWEAYPGDEDNGSLSAWYIWSALGFYPTCPGKPS
                     YDLGIPLFDHLRVYLAKEDKWLDIHTKQNHNHFNFVKECRLDKTLVSTIQHQDLLKAE
                     QLTFTLSWLPSH"
     gene            complement(11078..12757)
                     /locus_tag="VYN69_000938"
     CDS             complement(11078..12757)
                     /locus_tag="VYN69_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HEV1533232.1"
                     /translation="MKKIKPHGPLPSQTQLAYLGDELAAFIHFGPNTFYNQEWGTGQE
                     DPERFNPTQLDAREWVRVLKETGFKKLILVVKHHDGFVLYPTAHTDYSVKVSPWRRGK
                     GDLLLEVSQAATEFDMDMGVYLSPWDAHSPLYHVDREADYNAYYLAQLKEILSNPNYG
                     NAGKFAEVWMDGARGEGAQKVNYEFEKWFETIRDLQGDCLIFSTEGTSIRWIGNERGY
                     AGDPLWQKVNLDKLGTEAELNYLQHGDPSGTIFSIGEADVSIRPGWFYHEDQDPKSLE
                     ELVEIYFHSVGRGTPLLLNIPPNQAGLFDAKDIERLYEFATYRNELYKEDLALGAEVS
                     GPALSADFACRHLTDGLETSSWASDADLPIQLELDLGSPKTFDVIELREDLKLGQRIA
                     AFHVQVEVDGVWQEFGRGFTVGHKRLLRGPLVEAQKVRVMITEAQSIPVLTKISLYKT
                     PGLSKKEVVQELAFAEKSLAVAKGETLHFRIERSESSSSLEAKISIQPGTGVHGVAYQ
                     DEIYVLAFQAGETEKRLTLPTLYFAGDKTLDFYLNLTVDGQLVDQLQVQVS"
     gene            13513..14742
                     /gene="arcA"
                     /locus_tag="VYN69_000939"
     CDS             13513..14742
                     /gene="arcA"
                     /locus_tag="VYN69_000939"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000094613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEV1533233.1"
                     /translation="MSSHPIQVFSEIGKLKKVMLHRPGKELENLLPDYLERLLFDDIP
                     FLEDAQKEHDAFAQALRDEGIEVLYLEQLAAESLTSPEIRDQFIEEYLDEANIRDRQT
                     KVAIRELLHGIKDNQELVEKTMAGIQKVELPEIPDEAKDLTDLVESDYPFAIDPMPNL
                     YFTRDPFATIGNAVSLNHMFADTRNRETLYGKYIFKYHPIYGGKVDLVYNREEDTRIE
                     GGDELVLSKDVLAVGISQRTDAASIEKLLVNIFKKNVGFKKVLAFEFANNRKFMHLDT
                     VFTMVDYDKFTIHPEIEGDLHVYSVTYENEKLKIVEEKGDLAELLAQNLDVEKVHLIR
                     CGGGNIVAAAREQWNDGSNTLTIAPGVVVVYDRNTVTNKILEEYGLRLIKIRGSELVR
                     GRGGPRCMSMPFEREEV"
     gene            14800..15816
                     /gene="argF"
                     /locus_tag="VYN69_000940"
     CDS             14800..15816
                     /gene="argF"
                     /locus_tag="VYN69_000940"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEV1533234.1"
                     /translation="MTNSVFQGRSFLAEKDFTRAELEYLIGLSAHLKDLKKRNIQHHY
                     LAGKNIALLFEKTSTRTRAAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQRMVEELAEFSGVPVWNGLTDEWHPTQMLADYLTVQENFGRLEGLTLV
                     YCGDGRNNVANSLLVTGAILGVNVHIFSPKELFPEKEIVELAEGFAKESGAHVLITED
                     ADEAVKDADVLYTDVWVSMGEEDKFAERVALLKPYQVNMDLVKKAGNENLIFLHCLPA
                     FHDTHTVYGKDVAEKFGVEEMEVTDEVFRSKYARHFDQAENRMHTIKAVMAATLGNLY
                     IPKV"
     gene            15981..16928
                     /gene="arcC"
                     /locus_tag="VYN69_000941"
     CDS             15981..16928
                     /gene="arcC"
                     /locus_tag="VYN69_000941"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003025409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEV1533235.1"
                     /translation="MANRKIVVALGGNAILSSDPSAKAQQEALVETAKHLVKLIKNGD
                     DLIITHGNGPQVGNLLLQHLASDSEKNPAFPLDSLVAMTEGSIGFWLKNALQNALLDE
                     GIEKNVASVVTQVVVDKNDPAFVNLSKPIGPFYSEEEAKAEAEKSGATFKEDAGRGWR
                     KVVASPKPVDIKEIETIRTLLNNGQVVVAAGGGGIPVVKENNGHLTGVEAVIDKDFAS
                     QRLAELVDADLFIILTGVDYVFVNYNKPNQEKLEHVNVAQLEEYIKQDQFAPGSMLPK
                     VEAAIAFVNGRPEGKAVITSLENLGALIESESGTIIEKG"
     gene            17139..18650
                     /locus_tag="VYN69_000942"
     CDS             17139..18650
                     /locus_tag="VYN69_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004191872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEV1533236.1"
                     /translation="MSEKAKKGFKMPSSYTVLLIIIAIMAVLTWFIPAGAFIEGIYET
                     QPQNPQGIWDVLMAPIRAMLGTHPEEGSLIKETSAAIDVAFFILMVGGFLGIVNKTGA
                     LDVGIASIVKKYKGREKMLILVLMPLFALGGTTYGMGEETMAFYPLLVPVMMAVGFDS
                     LTGVAIILLGSQIGCLASTLNPFATGIASATAGVGTGDGIVLRLIFWVTLTALSTWFV
                     YRYADKIQKDPTKSLVYSTRKEDLKHFNVEESSSVESTLSSKQKSVLFLFVLTFILMV
                     LSFIPWTDLGVTIFDDFNAWLTGLPVIGNIVGSSTSALGTWYFPEGAMLFAFMGILIG
                     VIYGLKEDKIISSFMNGAADLLSVALIVAIARGIQVIMNDGMITDTILNWGKEGLSGL
                     SSQVFIVVTYIFYLPMSFLIPSSSGLASATMGIMAPLGEFVNVRPSLIITAYQSASGV
                     LNLIAPTSGIVMGALALGRINIGTWWKFMGKLVVAIIVVTIALLLLGTFLPFL"
     gene            18672..20003
                     /locus_tag="VYN69_000943"
     CDS             18672..20003
                     /locus_tag="VYN69_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HEV1533237.1"
                     /translation="MKIDITNQVKDEFLISLKTLISYPSVLNEGENGTPFGQAIQDVL
                     EKTLEICQDIGFTTYLDPKGYYGYAEIGQGAELLAILCHLDVVPSGDEADWQTPPFEA
                     TIKDGWVFGRGVQDDKGPSLAALYAVKSLLDQGIQFKKRVRFIFGTDEETLWRCMARY
                     NTIEEQASMGFAPDSSFPLTYAEKGLLQVKLHGPGSDQLELEVGGAFNVVPDKANYQG
                     PLYEQVCNGLKEAGYDYQSTEQTVTVLGVPKHAKDASQGINAVIRLATILAPLQEHPA
                     LSFLATQAGQDGTGRQIFGDIADEPSGHLSFNVAGLMINHERSEIRIDIRTPVLADKE
                     ELVELLTRCAQNYQLRYEEFDYLAPLYVAKDSKLVSTLMQIYQEKTGDNSPAISSGGA
                     TFARTMPNCVAFGALFPGAKQTEHQANECAVLEDLYRAMDIYAEAVYRLAT"
     gene            20098..>20188
                     /locus_tag="VYN69_000944"
     CDS             20098..>20188
                     /locus_tag="VYN69_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1533238.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGV"
BASE COUNT         5791 a         4512 c         4129 g         5756 t
ORIGIN      
        1 taatttcttt tttaacccgg gttaaaaaag aaattaaagt gggttaaaaa agaaattaac
       61 tgagtaaaaa ctctcgaaat tcaattcgct atattatcac tcaacttgaa caaataccag
      121 aaccacaaaa aacagatgta aaggaaaaat atggacttaa aatttgaagg ggttgattta
      181 gaatacaaaa aagctaaaaa caatcttcca gagtctttct gggaaaccta ctcagcattt
      241 gcaaatacaa atggcgggaa aataattctc gggatagatg aaaaaaacat tgacacctac
      301 caaagagtca atagactccc tgcgaaatta tgaagcaagt aaacaactaa ccgatgcacg
      361 atttaagcgt cttgttggtg ttcagcgcac cacttttgaa gagatgttag ctgtgttaaa
      421 aacaacttat caacttaaac acgcaaaagg tggacgaaaa cctaaattaa gcctagaaga
      481 ccttcttatg gccactcttc aatatgtgcg agaataccgc acttatgaag aaattgcggc
      541 tgattttggt attcacgaaa gcaacttaat ccgtcggagc caatgggttg aagcaactct
      601 tgttcaaagt ggtgttacga tttcaagaac tcctctcagt tctgaggaca cggtaatgat
      661 tgatgcgacg gaagtaaaaa tcaatcgccc taaaaaaaga actagcgaat gattctggta
      721 aaaagaaatg ccacgctatg aaggctcaag cgattgtcac aagtcaaggg agaattgttt
      781 ctttggatat cactgtgaac tattgtcatg atatgaagtt gttcaaaatg agtcgcagaa
      841 atatcggaca agctggtaaa atcttggctg acagtggtta tcaagggccc atgaagatat
      901 atcctcaagc acaaactcca cgtaaatcca gcaaactcaa gccgctaata gctgaagata
      961 aagcctataa ccatgcgcta tctaaggaga gaagcaaggt tgagaacatc tttgccaaag
     1021 taaaaacgtt taaaatattt tcaacaacct atcgaaatca tcgtaaacgc ttcggattac
     1081 gaatgaattt gattgctggt attatcaatc atgaactagg attctagttt tgcaggaagt
     1141 ctattgaaat actaagccta cacacagact gattccaatt gttttttaga tttcaatttt
     1201 accattcttc atagtcaaaa ccatcataat ctggatattg ttctggcgac gttccaaccg
     1261 aacgtacgag ataagcttct tcgtcctctg tctcgatttc tctcagcact tggcattcaa
     1321 aacgccattc atcaccgaag tcgaaaataa acttaaattt ttgtttgaca cttaggctat
     1381 ccagatagac attttctgtg taacgttctt caacatcgtc actaacaaag ctaagaaagt
     1441 aagaatctgc atgactccac tcaacattat ccatgaaaaa tgtgtgagca tgatcatttg
     1501 caaaatcaaa actccataaa atcacatctg ctagttccgc taacgttttt gtttttggaa
     1561 tctatatatg tcggtagcaa ccttttttaa atttgacgga aatgatccaa gcttctacag
     1621 atgctggctt ttcagcttct atttcttgtt ttactaattt taagtgtagc ataatacctc
     1681 caattgctca tcgatagaat tgctcttagg aaacattcac tttctgctat tttatcataa
     1741 gtttgtctaa ctgtagcatt tgaaaccaga taattcttct gtttcacgcg aaacaccctg
     1801 cgccaagcaa ggtgttcttg tttttatact caatgaaaat caaagagcaa actaggaagc
     1861 tagctgcagg ctgctcaaaa cactgttttg aggttgtaga tggaagctga cgtggtttga
     1921 agagattttc gaagagtatt aagtcgtata aatcgtcgac gctgtcgcaa tggtatgcca
     1981 ctggttggct gtagttgctg cgaaatcctt gtctgcgtag aagtcagtaa atggtaggat
     2041 ttcaacttcc agctcgtcga tgcggtcaag ctgaccagcc agataaaact cgatacggct
     2101 ttctgctcgt ttgatgcgtt gcaagagtcc gcccatacgg atgtcaactg tatccaaacc
     2161 aaagaccttg ttttctttca gccattggtg actaaagagg gcatggaagt cttcaatttg
     2221 gcttctaagt tctggtaatt cttgtctggc gatttgttgt aaactttctt tatcatccgc
     2281 ttggtaggcc tgacgaatgc gtcgtcccac atctactttg ctacttaaaa tagcattcaa
     2341 ctgggcctga gtttcaaaga gataggcata gtttccagct ttttctttaa tgttagcaag
     2401 cgtctcagca gcctgagcga agtgcggttt gtcctgttca ggtgtcatgt gttgatcaag
     2461 aatcggacaa agaatatcct gataaaaaac atagcggttg ggattgatac cgctgagatt
     2521 gcctggtagg tctggtaaga ggttggcaag gtcaatctgc ataaaatcct caaccgttag
     2581 accagtattg gtcttgaagt gcgcagacaa accatctagg tcattgcgat agctgagttc
     2641 tgcccagatt tgcaagcttg gtaggataga gaactgggca gtttcaccac cattgtctcc
     2701 ccaacccgtt acgatgactt ctttaatctg attggcacgg caggctttat tagcctcgat
     2761 agccactaga cggctaaaat ggttgtgagg tgtaaagcca atccacttcc aagctccccc
     2821 tgcaaatgca aggtcatggc taatcttgtg atgattgcgg aaattacggt tgtatttttc
     2881 ctcgctatcc tgataataat cccagtaaac cagagtcaca cggtctttga gacggtctag
     2941 gtagacacga gtttcctctg gaatttccac atcacgatcg tactggccat ccgctgacat
     3001 gagtttgaag aacatatcac tccacatctg gcagtggaaa ccatatttgt cagcaatatc
     3061 cagcacgcgc tccaagtgtt ggcacatgag gagactacga tccacaacac cgttcaaaat
     3121 caggtagcgt cccaaaccaa ccaagtgggc ttcgtccatc ccgatattga ccttgcgagt
     3181 cttcagttta gacaacgtgg caaacatgcc atcaatcagg tcataaacct tttcttcgcc
     3241 aatgagaaga atgttctcta catcacggag ctcctgcact tccttgacac cccatttgac
     3301 aaaggccgac aagtgggcca aggtctggat gcatggtaca aaggtcatgt caaactgttg
     3361 agcataggct tctatttcct gcaactcctc tgctgaataa gctccacgga aatagccaaa
     3421 gtaaggctgt ccttcaatct ggtaagtgtc ttccatgtaa agctcaaagg ttgagtagcc
     3481 catgagagcc aagacctcaa tcatctgctt ggcagaagcc acattcagca ccgcatttcg
     3541 agaacagtca accatgtaag ccaaatcttc gtaagctgct tgttcctcaa tctctacttt
     3601 atcagcttct gctagaactg ttaccaacaa ggacaaggca cgataaagtt ggtgaggttt
     3661 gcggtaggtc aattgatagt gaccttcctc accctggata gagatagagg cttggtcaga
     3721 ctgagtgaca gccacttcca catctggtag agaaatgtga ccttttaaaa cctcaatagc
     3781 ttgcgtttgt ttgggactaa gtcctgtaaa tcttaccatt gcttttcctc ctgtagccag
     3841 ttgacaaggg caccgtagag attggcatct gcgtgatagg tgcaggcctg gataactggt
     3901 gcgaccgtgt attcttcgta ggcatcgaca aagtcttcaa cagccttctt aacaccttgg
     3961 ataaaatctg gattttgact gatagagcca cccagactga tgacatctgg atcgatgaga
     4021 tactggatat tgagcaagcc ttgcgtcaga ttgcggttca tgcgctcaat ggcttcttga
     4081 caaagagcat taccagctgc ggcctcttgg taaatcttgc gaccgtccca atcagtatga
     4141 ccagattttt caatcacgta tcgtaccata ttcccagttg atgctagttg cgaccagtta
     4201 ttaagttttt cagcaggggc aagggttgtc atgtagccaa attctccacc cagaccgtgg
     4261 cgacctcgat gaagtctacc attgataatc atggctccgc caatccctgt cccaatcacg
     4321 acacaggctg cattttcaag ctctggatga gctagtagtt cactgagtcc aacgcagttg
     4381 gcatcatttt ctaaatggac aggtagctga taagagctaa gcgcctcata ccaagaaaag
     4441 ccatggatat agggcaccgc actgaagcca tcaatcacac ctgtctcttg attgaccgca
     4501 cctggaacgc tcatagcaat cccactgtaa tcctgttctg acaagcgttg atctagccac
     4561 gttagtaaat cctccaagtt ttcaggcgtt gaaatacttg tcttatccag tattttccca
     4621 tcaggagtca gactggcaaa cttaatccca gtccctccga tatcaatcgt tgcaatggtc
     4681 attgttttta ccataaaaag gggcgaatca gtagttagtt cttacctttt cgcccctcct
     4741 ttcttttgat gtttactttt tgaaattaaa tttcttcttt tttgatgaat tctgtacgaa
     4801 tctcttgtgg agccaatagt cctgaatgaa ctggatatgg tcgctcaagt aagtcaagaa
     4861 tggtttgttg atgttctgat atgcgcacat tttcttgact catattatag taacggagga
     4921 catagccttc ttcattttcc gccactttaa aggctgttgg acaaacttgc ggtatgctga
     4981 gaacagaatg gctcaagagg ctaccagtcg caaccacgct tccttcctgt ctagcaagct
     5041 gaaggctggt aaacggtgtc tgcaaggctt tggcacgacg ataggctgag aagcgttctt
     5101 gggcttggtg gcattcaatc gcaaattcga cttcaaactc ccgcaagcat tgtgcttctg
     5161 gcgttgggaa gtagccccag tcacctagct cacctgatgc acgcaaaatg gtcacggcaa
     5221 tggtgttatc cccaaggatt tcgtattcat tcaatccctt attggataca gtcacccctt
     5281 tttcatcgtc atacagactg acaaaagctt gttggtgttg aggattttca ggattttccc
     5341 atgaagcagc tggtttgttt ggtcgtgtca ccacctcata gatactttca gaatcattac
     5401 ttggacgcgt gttatgagtc ttgaccaaga gacggatacg gtgatccttg gcagtgttag
     5461 taaagcgagt cttgaagcgg atttgtggat tgtcaacgaa gacagtcaac tcagtttcca
     5521 gaggaatgtt tgtcaattct tctgaccgtc cagcctcacg cttcataaac tcgatgatac
     5581 cttgttgctc ttcttctagc ttttcatccg cgctgacagg cacggtcaat tcatgtttga
     5641 gcaagatttt agcatagcaa gctgtgtttt ccaagacctc gtggccctta agctctgcaa
     5701 agattggctc tgttcctttt ggttggaaat agatatactc gtttccgatg tccccacggt
     5761 cttcaaagcg gataaagtct tcataggctt cgtgagttgt cttatcatag actgtgatgt
     5821 tgtcatccat actcaccgtt acgaatggtg tatcaatcac tccgttttgg taaataccct
     5881 cacggtgttc ttgttctcct tccagcaatt ggaaggttgt ccaagaaagc ggtgctaggt
     5941 gaactggaat ggtcacgcgc acttgtcgag caatacgagc ttggcggaac ttgtcttttg
     6001 gtaaatcgta ttcaaaatta gctccgaggt cttcgattgt agcctctaca ggacgaccat
     6061 ccaagtcctc cacacggtaa cttggcaaga taagagcagc catctttttg tagccttctg
     6121 ttgggtgcaa ttccttgaaa tcacaagtcg ccacatcaat cactgtgctg acagtatcga
     6181 ccttatcatg caagcctgtg ttaatgacag taaagagata gtcactttga gccttatccg
     6241 tagcaatttt acccttccac tcgttgagca aattactttt aacaaagttt cctacttggt
     6301 tgaccttggc aaaacgcgtt tccatctcgc ggtgaacttc gtccacgcta cagccacaga
     6361 tactatcatg tggcgcattc tgcaaaagtg ttttccaagc ataggtcaac tggtccttgt
     6421 ggttgtgtcc accagtgata atagtcaagg gttctaccac ttgctctagg aggttgctat
     6481 tttcttggaa ggcttgtttt aggtaaatgc gggatgaaga agtgttggca agtgtgtacc
     6541 agccatctgt ttcctgactg gtcaactcac ctgtaacagt tgataagtgt tcaggaagcg
     6601 caccttctac agcttgaaca tattcatcaa aggaactatg aacaaagatt acatccggga
     6661 agagttcatt tgccacacga atggcttcgc tcagattttt ctgtacaggc tggtggtcac
     6721 agccgttcat catcaaccat tggttggtcg aagcgtaggc acgcacatct gacaattttt
     6781 gtttccagaa ggtcaaggcc tcatctttgt caactggaat ttcattcccg ttactgtacc
     6841 agttggcaaa gagaatacct aaaacacgac taccatccac accctgccag tacatttctg
     6901 aaaactggga tgtaaactgc tcatcttcaa ggacttggtt gtcaaatcca atcggcttca
     6961 caccacgacc aaaggtcgcc acgtgaatgc ctgatttttg aagaatttga ggcgcttgtc
     7021 ccatatttcc aaaggtatct ggaaagtagc caatctgggt tgatttaccc catttggcag
     7081 cttcttgttg accaatcaag gtattgcgga cattggcttc actggagatc aaataatcat
     7141 cctgcaagat gtaaaaggga ccaattttaa gcttgccctc gtcaatgtag cgttggacct
     7201 tgtcacgatt ttcagggcga atttgtaagt agtcgtcaag gacaatagtt tgtccatcca
     7261 agtggaaact cttgaactca gggtcatttt caaagagatc aaagagattg tcaaacaatt
     7321 ccaccaactg catacgatga ctttcaaaag gcaagtacca ctcacggtcc cagtgactat
     7381 gtgagataat atgtacaaca acattttcca tgaagtaaaa cctcattcta aatttaaatt
     7441 ttaacgtttt aaatgtaaac ttgtgattct gataagaatc ggttgagcta aagcgagccc
     7501 cttagcgaat atccaagtaa tccaagacca actcacagaa catcatgtta gcccaggaga
     7561 accattcacg agagtagagg gtcggatcat ctacatgaaa gctttcgtgc atgacacctg
     7621 taccaccatc gcaggcaacc agctgatcca gcaagaattt tttctctgcc ttatctcttg
     7681 ttgtcaagcc ttggatagaa agggcgattg gccagatata gcgatagaag gtatgagaac
     7741 tgccgagacc gcttgcgtat tctccttggt agaagtatgg attttcagag ctcaaaatgg
     7801 tacgacgagt agcttgatac acttcatcat cgaccgaaca gtagcccaga tagggcgcag
     7861 ctagtagact tggtacattt ggatcatcca tgatgctggc atttcctagg ccatccactt
     7921 caaaagcgta aatcttttcg cccttgctgt tggtggtgta agcgtagttt ttgattcctt
     7981 cttggatttc atcctgaaga cgcttggcat cagcaataac actctggcta tcagctaggt
     8041 ttaatgctgc gaagatttct tgcacataac ccaagactac tacagcaaac atatttgacg
     8101 gaatcaagta actatactgg caacagtcat cactcggacg aaaagctgac caagtcatac
     8161 ctgtcactgc aaagtcaggt ccaaagccat catttaccaa ggtgtcttcc ttacggtccg
     8221 tatctcggac aaaacgataa ggagagttct tgtggtcttg ttccaccgtc cacagatgga
     8281 gaatttcctt agtcgctgcg acaaaaatct catcaaactg actagtctcg ccagtctctt
     8341 tccagaggag ataagccaac tgcaaaggat agcaaagcga atccacctca tacttgcgct
     8401 cccagatcca gccgttaagg tctgtgtggt cagtctcgtg gtgccctttc cagttctcct
     8461 caatgttgaa ggagttagca tagggatcct tgagtaccaa ggtcatctga cgtttgacca
     8521 aacctgcaat ggtctgacgc aggagggcat ctcttttagc tacatgaagg tagggtctga
     8581 gttgggctgt cgaatctcga agccacatgg caggaatatc cccagtcaag acaaaagttg
     8641 aaccatcttc taagatttca accgtattgt ccaaggtatc ggtgtagcaa cgctcgaaaa
     8701 catccaccca ctctggatag tccttagccc gctctgctac ttcatctagc cactctctga
     8761 cgatttcttt cgaataaacc attttaaatt ttacctcttt caaacaagtt tcatgttcag
     8821 tatatagctt ttgagacaga aaatacatac acaaatgata gtcatttttc tacaaaatag
     8881 ttatttttga aactcctttt ctaaaggccc tctttttctg atataatgta gaaaaacagc
     8941 taaaggaaag actatgaaac cactacttga aaccatcgat acccgctttg gaactaccaa
     9001 caagcatgcc ttttctcggg gaaataccct gccatacaca ggcgtgcctt ttgggatgaa
     9061 ttactttgtg ccccagacca gtgaccagga tggatcttgg ttttttgatc cacatctgcc
     9121 tatctttcag gggattcgat taactcacca gcccagtcct tggattgggg actactcttg
     9181 gctccttctg acacctgtca ccagtcaact gggtggagac agcctctttc accgccaatc
     9241 ttcctatgat agggataagg cctctttcca acctcattat ctaaagattt tctccctgcg
     9301 ctatcagatt gaaacccagc tcacaccgac ttgctacggt gcttctattc gtttgaagca
     9361 aaagcaaggc aaagccctct ccctctatct tcacgcaaca gatgaactga cagtagagca
     9421 agtagataag cggactcttg ccctgcgaca agaaggtaaa actgaaacta acaaaaattc
     9481 gctaacgatg ttcactgccc tgcaaatgaa cacggatatt cttgctatca gccaagaagg
     9541 gggagactgg cgaattgact tagcaagtag tcaaaccgag atgcaactag ccacttcttt
     9601 catctctcct tctcaagcac tgcttaatct acctcaagaa gattttgata gctgtaaatc
     9661 aagtgcccaa gcggattggg aaaatctcct ccatcgtttt gacattatag agataggaga
     9721 ggctgaccga accttctttg accactgcct ctacagactc ttcctcttcc cacagacttt
     9781 ttatgagatt aatgaatcag ggcaagccat ccacatggat ctggctactg gtactgtcaa
     9841 gcctggtgtc ctctttagca acaatggttt ctgggatacc ttccgcacca ccttccccct
     9901 ctttgccctt atcataccgg agcactatca acgcttttta gaaggtttcc tcaatagcta
     9961 ccgcgatact ggtttccttc caaaatggct ggctccagat gaacggggta tgatgccagg
    10021 tacactttta gacggtatta tcgcagatag cgcctgcaag gacatggccc ctgacctaga
    10081 aggagaactc ttccaagcca tgctcgaaac agccagcaaa gccgaccctc tcggaatcaa
    10141 tggccgccac ggactagccc aataccaaga actaggttac ctctctaccg accaccacga
    10201 aagtgttagc catactctag actatgccta tagtgacttt tgtatcgcca actgtgccaa
    10261 aaaactaaaa aacatagaaa tcgctgaaac ctacaaggct gcttcacaaa attaccgcca
    10321 gctatttgac gctgagacag gttacatgcg agcacgagac aatcaaggaa actttcaccc
    10381 tgacttctct ccttatagtt gggggcgaga ctacgctgaa tgctctgcca ttcaagctac
    10441 tttaggcgtt ctccacgaca tccctggctt aatccaactg atgggtggaa aagaaacctt
    10501 tagcaactat cttttgaaag cctgtcaaga tgctcccctc tttgaaacaa caggctatgg
    10561 ttacgaaatt cacgaaatga gcgagatggc tactgctcct tttggacaaa tagccatttc
    10621 caaccagcct agtttccaca ttccttatct cttccgttac agcgattacc ctgactacac
    10681 tgcccttctt atcaagacgc tccgtcagaa agcttttcac ccaagttggg aagcctatcc
    10741 tggagatgaa gacaatggta gtctctctgc ttggtacata tggtcagctc tcggatttta
    10801 tccaacctgt ccaggaaaac caagctatga cctcggaatc cctctctttg accatctacg
    10861 agtctacctc gctaaagaag ataaatggct ggatatccat actaaacaaa accacaacca
    10921 ttttaacttt gtcaaagaat gccgactgga caaaacactc gtatcaacta ttcaacacca
    10981 agacctctta aaagctgaac aactaacttt caccctcagc tggttaccaa gtcactaata
    11041 accaaaagaa cctttgcatc tcgcaaaggt tcttctttta tgaaacttgg acttgaagtt
    11101 gatccacaag ctgaccatcc accgttaggt tcagatagaa atccaaggtt ttatctcctg
    11161 cgaaatacaa ggttggtagc gtcagccttt tttcagtctc accagcttga aacgcaagga
    11221 cataaatctc gtcctgatag gcgacaccat ggacacctgt ccccggttga atcgaaatct
    11281 tagcctctaa agaggagcta ctttcactac gttcaatcct aaaatgaaga gtctctccct
    11341 ttgccacagc taggcttttt tctgcaaatg ctagttcctg aacaacttct ttttttgata
    11401 acccaggagt tttataaagg gaaatcttgg tcaatacagg gatagattgt gcctcggtaa
    11461 tcatcacgcg caccttttgt gcctctacta gcggacctcg caagaggcgc ttatgaccaa
    11521 ctgtgaagcc cctgccaaac tcctgccaga caccatccac ctctacttgc acatgaaaag
    11581 cagcgattcg ttgccctagc ttcaaatctt ctcttaactc aattacatca aaagttttag
    11641 gagaacctaa atcgagttct aactggatgg gcaagtctgc atcgcttgcc caagagctgg
    11701 tctcaaggcc gtctgtcaaa tggcgacaag caaagtctgc ggaaagagct ggaccagata
    11761 cctcagctcc cagagccaaa tcttctttat agagctcatt gcgataggtc gcaaattcat
    11821 aaagtcgttc aatatccttt gcatcaaaga gcccagcttg attcggcgga atattaagca
    11881 agagtggagt tcctcgccct actgagtgaa agtagatttc gaccaactcc tcgagagact
    11941 taggatcctg atcctcatgg tagaaccagc ctggacggat ggaaacatct gcctctccga
    12001 ttgaaaaaat cgtgcccgag ggatccccgt gctgaagata gttcagctct gcttctgttc
    12061 ctagtttatc aagattcacc ttttgccaca gtggatcacc tgcataccct cgttcattgc
    12121 caatccagcg gatactggtg ccttctgttg aaaaaatcaa gcaatcgccc tgcaggtcac
    12181 gaatggtttc aaaccatttt tcaaattcat aattaacctt ttgcgcgccc tctcctctgg
    12241 caccatccat ccaaacctca gcgaacttac cagcattccc atagttagga tttgataaga
    12301 tttccttcaa ctgagccaga taataggcat tgtagtccgc ttctcggtcc acatgataga
    12361 ggggactatg ggcatcccac ggtgacaggt agacccccat atccatatca aactctgtgg
    12421 cagcttggga tacttcaagg agcaagtcgc cctttcctct cctccaagga ctgaccttaa
    12481 ccgaataatc tgtgtgagct gtcggataaa ggacaaagcc atcgtggtgc ttgaccacca
    12541 aaatcaactt tttgaagccc gtttccttga gcacgcgaac ccactcacgt gcgtctaact
    12601 gtgtcgggtt aaagcgttca ggatcctcct gacctgttcc ccattcctga ttataaaaag
    12661 tattaggtcc aaaatggata aaggctgcta gttcatctcc cagataagct agctgagtct
    12721 gacttggtaa cggtccatgc ggtttgattt tcttcatcgt tatatatctc cttctttagc
    12781 aacttctctt gttcacaagg ttatccacag gttgtggata ataaaccatg atctaacagc
    12841 cagctctttc caactccaac tggtaggctc aggatttttc tcctcaatat tccctcttca
    12901 aacagctctg tcatatggtt ctgctaacac ctctcagaat gctactctcc ataattattc
    12961 tcaggcttct ttcaggtcat tgtgtgaaga ccattcactc tccagacttt tccacagatt
    13021 gtgaataagt gattattcta aactatcatc tcgtcgacta taacaaaaaa taaaaagagg
    13081 gtcaataaga taattatctc ttcctaaata ctcagaaaag attctataat ttgtacaatt
    13141 gagcaagaaa tgcttctcca aaatctatct tcaaaagaat gttgcatctt tttatccttt
    13201 cacacaaaca tttctcttta tacagatcca agtgataacc agatgataat tggacacttt
    13261 ttggttattt gtgtattttt tgaaaaattt tttaaaaaaa ttttatatcc tctttaatag
    13321 aagtttatgc atttgtatat atgtattcca tcctatgttg tgtataataa cataaaattg
    13381 tgctaaatat cataatttat attattttcc gagaaattat agccattgac attacctagg
    13441 aattgcgtta taaaatacat gtaagcggta cccaaaatgg tgccaagtca aaatttttaa
    13501 ggaggaaaat acatgtcttc acatccaatt caggtcttct cagaaattgg gaaactgaaa
    13561 aaagttatgt tacaccgtcc aggcaaggag ttagaaaact tgttgccgga ctatcttgaa
    13621 aggcttcttt ttgatgatat tcctttcttg gaagatgctc aaaaagaaca tgatgcattt
    13681 gcccaagctc ttcgcgatga aggaattgag gttctctacc tagaacaact cgctgctgaa
    13741 tcattgacct ctccagaaat ccgcgatcaa tttatcgagg aatacttaga cgaagccaac
    13801 atccgtgatc gtcaaaccaa ggttgctatt cgtgaattgc ttcacggcat caaggacaac
    13861 caagaattgg ttgaaaaaac aatggctggg attcaaaaag ttgaattgcc agaaattcct
    13921 gacgaagcta aagatctaac tgacttagtt gaatcagatt atccatttgc aattgacccg
    13981 atgccaaacc tctatttcac tcgcgaccca tttgcaacaa ttggaaacgc cgtatcgctt
    14041 aaccacatgt ttgcagacac tcgtaaccgt gaaacactct acggtaagta tatcttcaaa
    14101 taccacccaa tctatggagg aaaagtggat ttggtctaca accgtgaaga agatacgcgt
    14161 atcgaaggtg gagacgagct agttctttct aaagacgtcc ttgcagtagg tatctctcaa
    14221 cgtacagacg cagcttctat agaaaaactt ttggtcaaca tcttcaagaa aaatgttggc
    14281 ttcaagaaag ttttggcctt tgaatttgct aacaaccgta aattcatgca cttggatact
    14341 gtcttcacta tggtagacta tgacaagttc actattcacc cagaaatcga aggcgacctt
    14401 cacgtttact cagttactta cgaaaacgaa aaacttaaaa tcgttgaaga gaaaggtgac
    14461 ttagctgaac ttcttgctca aaaccttgat gtagaaaaag ttcatttgat tcgttgcggt
    14521 ggtggcaata tcgtagcagc tgcgcgtgaa caatggaacg acggttctaa cactttgacc
    14581 atcgcacctg gtgtggtagt tgtttatgac cgcaataccg tgaccaataa gattttggaa
    14641 gaatacgggc ttcgcttgat taagattcgc ggaagtgaat tggttcgggg gcgtggtgga
    14701 cctcgttgta tgtctatgcc atttgaacgt gaagaagtgt aatcgctgtt cgatattcgt
    14761 caatagaaaa tgtaaaaaat agaaagagga aataataaaa tgacaaattc agtattccaa
    14821 ggacgcagct tcttagcaga aaaagacttt acccgtgcag agttagaata ccttattggt
    14881 ctttcagctc acttgaaaga tttgaaaaaa cgcaatattc aacaccacta ccttgctggc
    14941 aagaatatcg ctctcctatt tgaaaaaaca tctactcgta ctcgtgcagc ctttacaact
    15001 gcggctatcg accttggtgc tcacccagaa tacctcggag caaatgatat tcagttgggt
    15061 aaaaaagaat ctactgaaga tactgctaaa gtattgggac gtatgtttga cgggattgaa
    15121 ttccgcggat tcagccaacg tatggttgaa gaattggcag aattctcagg cgttccagta
    15181 tggaacggtc taactgacga atggcaccca actcaaatgc tcgctgacta cttgactgtt
    15241 caagaaaact tcggtcgctt ggaaggcttg acattggtat actgtggtga tggacgtaac
    15301 aacgttgcca acagcttgct cgtaacaggt gctatccttg gtgtcaatgt tcacatcttc
    15361 tcaccaaaag aactcttccc agaaaaagaa atcgttgaat tggcagaagg atttgctaaa
    15421 gaaagtggcg cacatgttct catcactgaa gatgctgatg aagcagttaa agatgcagac
    15481 gttctttaca cagacgtttg ggtatcaatg ggtgaagaag acaaattcgc agaacgtgta
    15541 gctcttctta aaccttacca agtcaatatg gacttagtta aaaaagcagg caatgaaaac
    15601 ttgatcttcc tacactgctt gccagcattc cacgatactc acactgttta tggtaaagac
    15661 gttgctgaaa aatttggtgt agaagaaatg gaagtaacag acgaagtctt ccgcagcaag
    15721 tacgctcgcc acttcgatca agcagaaaac cgtatgcaca ctatcaaagc tgttatggct
    15781 gcgacacttg gtaaccttta tattcctaaa gtataatttt agataataaa ccgtctacca
    15841 acagctatga gggctgcgac taatagcttt agtccggtcc tcttttatgt aatggtaatc
    15901 tattatttct tataaaatat gtgaaaaatc attaaattga aatctaaacg cattctattg
    15961 agtgtgataa aggagaattt atggcaaatc gtaaaattgt agtagctttg ggaggaaatg
    16021 cgattctttc ttctgaccca tcagcaaagg ctcaacaaga agctttagtt gaaacagcta
    16081 agcatcttgt aaaattgatt aaaaatggag atgatttgat tatcactcac ggtaatggac
    16141 ctcaagttgg gaatctcttg ctccaacatt tggcatcaga ctctgaaaag aaccctgcct
    16201 tcccactcga ctcacttgtc gctatgacag aaggtagcat cggtttctgg ttgaaaaatg
    16261 ctttgcaaaa tgctctcttg gatgaaggca tcgaaaaaaa tgttgcctct gttgtaacgc
    16321 aagttgtcgt agataaaaat gatccagctt ttgttaactt gagtaaacca atcggtcctt
    16381 tctattcaga agaagaagca aaagcagaag ccgaaaaaag cggagcgact ttcaaggaag
    16441 atgctggccg tggctggcgt aaggtcgttg cctcaccaaa acctgttgac atcaaagaaa
    16501 ttgaaaccat ccgtactctt ttaaataatg gtcaagtcgt cgtagctgca ggtggtggcg
    16561 gtattcccgt cgtcaaagaa aacaatggac atttgactgg tgtcgaagcg gttattgata
    16621 aagacttcgc ttcccaacgt ttggcagaat tggttgatgc agacctcttc atcattttga
    16681 caggtgtaga ttatgtattt gttaactaca acaagccaaa ccaggaaaaa ttggaacatg
    16741 tgaatgttgc ccagctggaa gaatatatca aacaagatca gtttgcacca ggtagcatgc
    16801 ttccaaaagt agaagcagct atcgcttttg tcaatggtcg tccagaagga aaagcagtta
    16861 ttacttccct tgaaaatcta ggcgccttga ttgaatctga aagtggaaca attattgaaa
    16921 aaggataagt tgttttacta ataagatgta ttctatttct agtatcttta tatcaaatta
    16981 gaaattattc ttgaaaacat gtacaatatt tcaaaagata ttagtttttt agactttaat
    17041 atggtaaaac aaatataaat agaaagcgtt ttcttaaatg tttatttaag aaagtagttg
    17101 gttttttaca ctttgttaga catcaggagg aaaaacaaat gagtgaaaaa gctaaaaaag
    17161 ggtttaagat gccttcatct tacaccgtat tattgataat cattgctatt atggcagtgc
    17221 taacttggtt tatccctgcg ggggccttta tagaaggtat ttacgagact cagcctcaaa
    17281 atccacaagg gatttgggat gtcctcatgg caccgattcg ggctatgcta ggtactcatc
    17341 cagaggaagg ttcgctcatt aaagaaacga gcgcagcgat tgatgtagcc ttcttcatcc
    17401 ttatggttgg tggtttcctt ggcattgtca acaaaactgg tgctcttgac gtagggattg
    17461 cctctatcgt gaagaagtat aagggccgcg aaaaaatgtt aattttggta ctgatgcctt
    17521 tgtttgccct cggtggtaca acttatggta tgggtgaaga aacaatggct ttctatccac
    17581 tccttgtgcc agttatgatg gccgttggtt ttgatagcct gactggtgtt gcaattattt
    17641 tgctcggttc tcaaatcggc tgtttggcat ctactctgaa tccatttgcg acaggtattg
    17701 cttcagcgac tgcgggagtt ggtacagggg acggtatcgt acttcgtctg atcttctggg
    17761 ttaccttgac tgctcttagt acttggtttg tttaccgtta tgcggataag attcaaaaag
    17821 atccgactaa gtcactggtt tatagtactc gcaaagaaga tttgaaacac tttaacgtgg
    17881 aagaatcttc atctgtagaa tctacactta gcagcaaaca aaaatcagtt ctcttcttat
    17941 ttgtgttgac attcatcttg atggtattga gcttcattcc atggacagac cttggcgtta
    18001 ccatttttga tgactttaat gcttggttga ctggtcttcc agttattggt aatattgtcg
    18061 gttcatctac ttctgcacta ggtacttggt acttcccaga aggcgcaatg ctctttgcct
    18121 ttatgggtat cctgattggt gttatttatg gtcttaaaga agataagatt atctcttcct
    18181 tcatgaatgg tgctgctgac ttgctcagtg ttgccttgat cgtagcgatt gctcgtggta
    18241 ttcaagttat catgaacgac ggtatgatta ccgatacaat cctcaactgg ggtaaagaag
    18301 gcttgagcgg tctatcttca caagtcttta tcgttgtaac ttatatcttc tatctaccta
    18361 tgtcattctt gatcccatct tcatctggtc ttgccagcgc aactatgggt atcatggctc
    18421 cacttggaga atttgtaaat gtccgtccta gcttgattat cactgcttac caatctgctt
    18481 caggtgtctt gaacttgatt gcaccaacat ctggtattgt gatgggagct cttgcacttg
    18541 gacgtatcaa cattggtact tggtggaaat tcatgggcaa actcgtagtc gctattattg
    18601 tagtgaccat cgcccttctt ctccttggaa ccttccttcc attcctataa aatagtgagt
    18661 gaggtgattc catgaaaata gatataacaa atcaagttaa agatgaattt cttatatcat
    18721 taaaaacctt gatttcctat ccttcagtac tcaatgaagg agaaaatgga acaccttttg
    18781 gacaagcaat ccaagatgtc ctagaaaaaa ctttagagat ttgtcaagac ataggtttca
    18841 ctacctatct tgaccctaaa ggttattacg gatatgcaga aatcggtcag ggagcagagc
    18901 ttctggccat tctctgtcat ttggatgttg ttccatcagg tgatgaagca gattggcaga
    18961 caccgccatt tgaagcaact atcaaagacg gctgggtatt cggacgtggt gtccaagatg
    19021 ataaaggccc ttcgctcgca gctctctatg cagtaaaaag cttgctggac caaggtattc
    19081 agttcaaaaa gcgcgtacgc tttatctttg gtaccgatga ggaaaccctc tggcgctgca
    19141 tggcacgcta caataccatc gaagaacagg ccagcatggg ctttgcacct gactcatctt
    19201 ttcctctgac ctatgctgaa aaagggcttc tacaggtcaa acttcatggc cctggatcgg
    19261 atcaactaga gcttgaagta ggaggcgcct ttaacgttgt accagacaag gccaactacc
    19321 aaggtcccct ctatgaacag gtttgtaacg gtctcaaaga agctggttat gattaccaat
    19381 ccactgaaca aaccgtaacg gttctcggag tgccaaagca tgctaaggat gctagtcaag
    19441 gtatcaatgc tgtcatccga ctagctacca ttcttgctcc tctccaagaa caccctgctc
    19501 tcagttttct tgcaacacaa gcaggtcaag acggcacagg aagacaaatc tttggtgata
    19561 tagcagatga accttctggt cacctatcct ttaatgtcgc aggtctcatg atcaatcatg
    19621 aacgttctga aatccgtatt gacattcgga ctcctgtctt agctgataag gaagaactag
    19681 tagagttgct tacaagatgt gcacaaaact accaactccg ctacgaagag tttgactatc
    19741 tagcgcctct atacgtcgca aaagacagta aactcgttag cacactgatg caaatctacc
    19801 aagaaaagac tggcgataac agtcctgcta tttcatccgg tggtgccact tttgctcgca
    19861 ccatgccaaa ttgtgtagcc ttcggcgcct tattcccagg agcgaagcag acagaacatc
    19921 aggcaaatga atgtgccgtt ctagaagatt tgtaccgtgc tatggatatt tatgccgaag
    19981 ccgtctatcg acttgcaact taatcaggca actgtttcta ccaaaaaaaa tcgaccgatt
    20041 aatgaactgc accccaaaag ttagacagaa taaatctaac ttttggggtg ttttattatg
    20101 aaattgagtt atgaagataa agttcagatc tatgaactaa gaaagcaagg acaaagcttc
    20161 aaacagcttt caaaaagatt tggtgtgg
//
LOCUS       DAVLVP010000027        20081 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.027, whole genome shotgun sequence.
ACCESSION   DAVLVP010000027 DAVLVP010000000
VERSION     DAVLVP010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 20081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20081
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.027"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..68
                     /locus_tag="VYN69_000945"
     CDS             <1..68
                     /locus_tag="VYN69_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lactate oxidase"
                     /protein_id="HEV1533239.1"
                     /translation="IPKAAFGYIASGAEDTFTSFQ"
     gene            195..1685
                     /gene="lysS"
                     /locus_tag="VYN69_000946"
     CDS             195..1685
                     /gene="lysS"
                     /locus_tag="VYN69_000946"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003055423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEV1533240.1"
                     /translation="MSTEHMEELNDQQIVRREKMAALREQGIDPFGKRFERTANSQEL
                     KDKYANLDKEQLHDKNETATIAGRLVTKRGKGKVGFAHLQDREGQIQIYVRKDAVGEE
                     NYEIFKKADLGDFLGVEGEVMRTDMGELSIKATHITHLSKALRPLPEKFHGLTDVETI
                     YRKRYLDLISNRESFERFVTRSKIISEIRRYLDQKGFLEVETPVLHNEAGGAAARPFI
                     THHNAQNIDMVLRIATELHLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYQ
                     AYADFQDIMDLTEGIIQHAAKSVKGDGPVNYQGTEIKINEPFKRVHMVDAIREITGVD
                     FWQDMTLEEAKAIAAEKKVPVEKHYTEVGHIINAFFEEFVEETLIQPTFVYGHPVAVS
                     PLAKKNPEDQRFTDRFELFIMTKEYGNAFTELNDPIDQLSRFEAQAKAKELGDDEATG
                     IDYDYIEALEYGMPPTGGLGIGIDRLCMLLTDTTTIRDVLLFPTMK"
     gene            2310..3446
                     /gene="lctO"
                     /locus_tag="VYN69_000947"
     CDS             2310..3446
                     /gene="lctO"
                     /locus_tag="VYN69_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate oxidase"
                     /protein_id="HEV1533241.1"
                     /translation="MSYKTSNAEGHVDFINTYDLEPMAQQVIPKAAFGYIASGAEDTF
                     TLRENIRAFNHKLIVPHTLCDVENPSTEIEFAGEKLSSPIIMAPVAAHKLANEQGEVA
                     TARGVHEFGSLYTTSSYSTVDLPEISEALQGTPHWFQFYFSKDDGINRHIMDRVKAEG
                     YKAIVLTADATVGGNREVDKRNGFVFPVGMPIVEEYLPEGAGKSMDFVYKSAKQRLSP
                     RDVEFIAEYSGLPVYVKGPQCREDVERSLAAGASGIWVTNHGGRQIDGGPAAFDSLQE
                     VAEAVDRRVPIVFDSGVRRGQHVFKALASGADLVAIGRPVIYGLALGGSVGVRQVFEH
                     LNAELKTVMQLSGAQTIEDVKHFKLRHNPYNPTFPVDPRDLKLY"
     regulatory      3700..3788
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            4003..4671
                     /locus_tag="VYN69_000948"
     CDS             4003..4671
                     /locus_tag="VYN69_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000449895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TenA family protein"
                     /protein_id="HEV1533242.1"
                     /translation="METQDYAFQPGLTVGELLKSSQKDWQAAINHRFVKELFAGTIEN
                     KVLKDYLIQDYHFFDAFLSMLGACVAHADKLESKLRFAKQLGFLEADEDGYFQKAFKE
                     LKVAENDYLEVTLHPVTKAFQDLMYSAVASSDYAHLLVMLVIAEGLYLDWGSKDLALP
                     EVYIHSEWINLHRGPFFAEWVQFLVDELNRVGKNREDLTELQQRWNQAVALELAFFDI
                     GYDV"
     gene            complement(4821..4907)
                     /locus_tag="VYN69_000949"
                     /pseudo
     CDS             complement(4821..4907)
                     /locus_tag="VYN69_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            4998..5780
                     /gene="thiM"
                     /locus_tag="VYN69_000950"
     CDS             4998..5780
                     /gene="thiM"
                     /locus_tag="VYN69_000950"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000202454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1533243.1"
                     /translation="MTSLKLLKEKAPLVICITNDVVKNFTANGLVALGVSPAMSEFPA
                     DLEDLLKYAGGLLINIGTLTDENWKLYQAALKIAEKYNVPAVLDPVACGAGEYRKKVA
                     DDLINNYKLAAIRGNAGEIASLVGIDVASKGVDSAGVDNIDEIALAANEKFNIPIVVT
                     GEVDAIAVNGEVVTIHNGSAMMPKVIGTGCLLGAVVASFIGLEKGQELKSLETAMLVY
                     NIAGEMAEKRPNGHLPGTFKVEFINALYEITDEDVKEFKRVK"
     gene            5782..6411
                     /locus_tag="VYN69_000951"
     CDS             5782..6411
                     /locus_tag="VYN69_000951"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000468574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1533244.1"
                     /translation="MFHKELLKLYFICGTTTCQGKNLYTVVEEALKGGITLFQFREKG
                     ESALEGLEKLELAIQIKELCKKYNVPFIVNDDIDLAMEIDADGVHVGQDDIGVDEIRK
                     LMPDKIIGLSIRNEEEFQQSKVEYVDYVGVGPVFDTQSKDDAGGAIGYEGLELMRKLL
                     PQMPLVAIGGIQTKHIKDIIKTNVDGVSIISAISYAKNIEKTVREMSEQ"
     regulatory      6620..6708
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6970..7530
                     /locus_tag="VYN69_000952"
     CDS             6970..7530
                     /locus_tag="VYN69_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1533245.1"
                     /translation="MLKKWQLKDVILLAFLSIFFGGVFVGSGYVYNILSLLLTPLGLQ
                     AFANEILFGLWCMAAPIAAIFVPRVGSATIGEVLAALAEVLYGSQFGLGALLSGLVQG
                     LGSEFGFIVTKNRYESWLSLTANSIGVTLVSFVYEYIKLGYYAFSLPFVLSLLVVRFI
                     SVYFFCTILVRAIVKLYHQFATGGKA"
     gene            7531..8916
                     /locus_tag="VYN69_000953"
     CDS             7531..8916
                     /locus_tag="VYN69_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533246.1"
                     /translation="MGLELRAIQSPIFSEPFDFTFHAQAFTLLVGSSGSGKSSLFQVI
                     AQVSSLPYSGQVLIDGSEVSQLSIIARVQKVGILFQNPNHQFTMENLFEELIFTLENI
                     GYHLQEIDSKIAEVVQQCRCEAILHRPIHHLSGGEKQKAALAVLFAMNPRVYLLDEPF
                     ASIDRKSRIEILEILKELALDGKTVILCDHDLSDYKAYIDHMVELRDGKLREVFQIPS
                     YEMTQVASKEVASSPELFHMNRVTGELGNRPLFSIADFTFYQGISCILGDNGVGKSTL
                     FRSILQFQKYKGRIAWKGTVLKKKKSLYRDLTGVVQEAEKQFIRVSLREELRLDGPDS
                     ERNQRIFQALRYFDLEQAVDKSPYQLSGGQQKILQLLTILTSKASVILLDEPFAGLDD
                     RACHYFCKWIVEERNQGRSFLLISHRLDPLISVVDYWIEMTSQGLSHVKEVTITKPLT
                     SQSSNTQGEVR"
     gene            8918..9568
                     /locus_tag="VYN69_000954"
     CDS             8918..9568
                     /locus_tag="VYN69_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ABC transporter permease"
                     /protein_id="HEV1533247.1"
                     /translation="MVKVATQTPIISLFLLILSLETSFIPSIALNLSVVAFCILFMLY
                     YRRFKMLAWMIILAILPSFANYWAVQLHGDASQAVMLGTRAFVTVCIGLVFVSSISLK
                     ELLLYLAQKGLSRSWSYALIVVFNSFPLIQQEIKSLKEACLLRGQELHFWSPLIYSKV
                     LMTVFRWRHLYLRALSAHGYDEHAQLKNSYRTFYIPKKTKLIYLLFFLLLQTSLFL"
     gene            9579..10271
                     /gene="tenA"
                     /locus_tag="VYN69_000955"
     CDS             9579..10271
                     /gene="tenA"
                     /locus_tag="VYN69_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HEV1533248.1"
                     /translation="MEFTDIAMELSKKAWQASFHHPFILQLQEGNLEPAIFRYYLIQD
                     AYYLKAFSEIYHLLADKTSNQEMKRLLKQNAQGLVEGELFIRQQFFKEMEISDQEMEQ
                     HPIAPTCYHYISHIYRQFAEPNLAIAFASLLPCPWLYHDIGKSLNLKPSPNPLYQQWI
                     ETYITDELEQQIREEGALVNQLYRESDETDKQKMLDAFHISVHMEAKFWEMAYQHQTW
                     KSDLQSLEKGEE"
     gene            10276..10800
                     /gene="thiW"
                     /locus_tag="VYN69_000956"
     CDS             10276..10800
                     /gene="thiW"
                     /locus_tag="VYN69_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HEV1533249.1"
                     /translation="MRKHQLQVHKLTILSMMIALDVVLTPIFRIEGMAPMSSVVNILA
                     GIMMGPVYALAMATVTAFIRMTTQGIPPLALTGATFGALLAGLFYKYGRKFYFSALGE
                     IVGTGIIGSIVSYPVMVLFTGSAAKLSWFIYTPRFFGATLIGTAISFIAFRFLIKQEF
                     FKKVQGYFFAERIE"
     gene            10800..11603
                     /locus_tag="VYN69_000957"
     CDS             10800..11603
                     /locus_tag="VYN69_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HEV1533250.1"
                     /translation="MQALTNPFPIGSSSLIHCMTNEISCEMLANGILALGCKPVMADD
                     PREVLDFTKQSQALFINLGHLSAEKEKAIRMAALYANQSSLPMVVDAVGVTTSSIRKS
                     LVKDLLDYRPTVLKGNMSEIRSLVGLKHHGVGVDASAKDQETEDLLQVLKDWCQTYPG
                     MPFLVTGSKDLIVSENQVAVLENGCTELDWITGTGDLVGALTAVFLSQGKTGFEASCL
                     AVSYLNIAAEKIVVQGMGLEEFRYQVLNQLSLLRRDENWLDTIKGEVYE"
     gene            11596..12228
                     /locus_tag="VYN69_000958"
     CDS             11596..12228
                     /locus_tag="VYN69_000958"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001078228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HEV1533251.1"
                     /translation="MNREALRLYLVTNRYQDSVESFLAKVETACRSGVTIVQLREKNL
                     TTNQYYQLAKQVKEITDAYQVPLIIDDRLDVCLAVDAAGLHIGDDELPVSVARQVLGP
                     EKILGVTAKTVKRALEAEKSGADYLGTGAIFPTTTKENAPITLISTLKTICQTVAIPI
                     VAIGGLTSENIDQLMGTGIAGVAVVRDLMQAEDIEAKTQAFLTKLHDILS"
     gene            complement(12437..13243)
                     /gene="thiD"
                     /locus_tag="VYN69_000959"
     CDS             complement(12437..13243)
                     /gene="thiD"
                     /locus_tag="VYN69_000959"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1533252.1"
                     /translation="MTYLPVALTIAGTDPSGGAGIMADLKSFQARDVYGMAVVTSLVA
                     QNTRGVQLIEHVSPQMLKAQLESVFSDIPPQAVKTGMLATTEIMEIIQPYLKKLDCPY
                     VLDPVMVATSGDALIDSNARDYLKTNLLPLATIITPNLPEAEEIVGFSIHDPEDMQRA
                     GRLILKEFGPQSVVIKGGHLKGGHLKGGAKDFLFTKNEQFVWESPRIQTCHTHGTGCT
                     FAAVITAELAKGKSLYQAVDKAKAFITKAIQDAPQLGHGSGPVNHTTFKD"
     regulatory      complement(13327..13425)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13609..14004
                     /locus_tag="VYN69_000960"
     CDS             13609..14004
                     /locus_tag="VYN69_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEV1533253.1"
                     /translation="MQISDAEWQVMKIIWMQGEQTSTDLIRVLAERFDWSKSTIQTLL
                     ARLVEKECLTRKKEGKFFVYSALLTLDQSRDLLVQDIKDKVCSRRIRNLLADLIVECE
                     FTQTDLEDLEAVISEKKSSAVTEVRCNCM"
     gene            14015..14386
                     /locus_tag="VYN69_000961"
     CDS             14015..14386
                     /locus_tag="VYN69_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000932058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HEV1533254.1"
                     /translation="MLNSIVTIICIALIAFILFWFFKKPEKSGQKAQQKNGYQEIRVE
                     VMGGYTPELIVLKKSVPARIVFDRKDPSPCLDQIVFPDFGVHANLPMGEEYVVEITPE
                     QAGEFGFACGMNMMHGKMIVE"
     gene            14396..16639
                     /locus_tag="VYN69_000962"
     CDS             14396..16639
                     /locus_tag="VYN69_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEV1533255.1"
                     /translation="MTEIVKASLENGIQKIRIRAEKGYHPAHIQLQKGIPAEITFHRA
                     TPSNCYKEILFEEEGILEPIGVDEEKVIRFTPQELGRHEFSCGMKMQKGSYIVVEKTR
                     KSLSLLQRFWITSIFTVPLVILMIGMLTGSISHQVMHWGTFLATTPIMLVAGKPYIQS
                     AWASFKKHNANMDTLVALGTLVAYFYSLVALFAGLPVYFESAGFILFFVLLGAVFEEK
                     MRKNTSQAVEKLLDLQAKTAEVLSDDSYVQVPLEQVKVGDLIRVRPGEKIAVDGVVVE
                     GVSSIDESMVTGESLPVDKTVGDTVIGSTINHSGTLVFRAEKVGSETVLAQIVDFVKK
                     AQTSRAPIQDLTDKISGIFVPVVVILGIMTFWVWFVLLRDSVVVLGASFVSSLLYGVA
                     VLIIACPCALGLATPTALMVGTGRSAKMGVLLKNGTVLQEIQKVQTLVFDKTGTLTEG
                     KPVVTDIIGDEVEVFGLAASLEDASQHPLAEAIVKRASEAGLEFKTVENFQALHGKGV
                     SGRINGKQVLLGNAKMLDGMDISNTYQDKLEELEKEAKTVVFLAVDNEIKGLLALQDI
                     PKENAKLAISQLKKRGLRTVMLTGDNAGVACAIADQIGIEEVIAGVLPEEKAHEIHKL
                     QQSGKVAFVGDGINDAPALSVADVGIAMGAGTDIAIESADLVLTTNNLLGVVRAFDMS
                     KKTFHRILLNLFWAFIYNVVGIPIAAGVFSGVGLALNPELAGLAMAFSSVSVLTSSLL
                     LNFSKID"
     gene            16846..18621
                     /gene="spxB"
                     /locus_tag="VYN69_000963"
     CDS             16846..18621
                     /gene="spxB"
                     /locus_tag="VYN69_000963"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000191798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEV1533256.1"
                     /translation="MTQGKITASAAMLNVLKTWGVDTIYGIPSGTLSSLMDALAEDKD
                     IRFLQVRHEETGALAAVMQAKFGGSIGVAVGSGGPGATHLINGVYDAAMDNTPFLAIL
                     GSRPVNELNMDAFQELNQNPMYNGIAVYNKRVAYAEQLPKVIDEACRAAISKKGPAVV
                     EIPVNFGFQEIDENSYYGSGSYERSFIAPALNEVEIDKAVEILNNAERPVIYAGFGGV
                     KAGEVITELSRKIKAPIITTGKNFEAFEWNYEGLTGSAYRVGWKPANEVVFEADTVLF
                     LGSNFPFAEVYEAFKNTEKFIQVDIDPYKLGKRHALDASILGDAGQAAKAILDKVNPV
                     ESTPWWRANVKNNQNWRDYMNKLEGKTEGELQLYQVYNAINKHADQDAIYSIDVGNTT
                     QTSTRHLHMTPKNMWRTSPLFATMGIALPGGIAAKKDNPDRQVWNIMGDGAFNMCYPD
                     VITNVQYDLPVINLVFSNAEYGFIKNKYEDTNKHLFGVDFTNADYAKIAEAQGAVGFT
                     VDRIEDIDAVVAEAVKLNKEGKTVVIDARITQHRPLPVEVLELDPKLHSEEAIKAFKE
                     KYEAEELVPFRLFLEEEGLQSRAIK"
     gene            18732..19079
                     /locus_tag="VYN69_000964"
     CDS             18732..19079
                     /locus_tag="VYN69_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003017645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1533257.1"
                     /translation="MNLNQLDIIVSNVPQVCADLEHILDKKADYADDGFAQFTIGSHC
                     LMLSQNHLVPLENFQSGIIIHIEVEDVDQNYKRLNELGIKVLHGPTVTDWGTESLLVQ
                     GPAGLVLDFYRMK"
     gene            complement(19196..20081)
                     /locus_tag="VYN69_000965"
                     /pseudo
     CDS             complement(19196..20081)
                     /locus_tag="VYN69_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT         5940 a         3739 c         4373 g         6029 t
ORIGIN      
        1 ttattcctaa agcagcattt ggctatatcg ctagtggggc ggaagatact ttcacttctt
       61 tccagtgatt ttagcgtcag gttcttttta gtttttaaag attatccgtg aatttcttgc
      121 ttatttatga taaaatgggg tatcgcaaaa aatgactcat cgtattcaat tttgagtaaa
      181 actaggagga tcccatgtct acagaacata tggaagaact aaatgaccag cagattgttc
      241 gccgtgaaaa aatggctgcg ctccgcgaac aaggaatcga tcctttcgga aaacgttttg
      301 aacgtactgc aaattcacaa gaattaaaag ataaatatgc caacctcgat aaagaacaat
      361 tacacgataa aaacgaaaca gctactatcg caggacgctt ggtaaccaaa cgtggtaaag
      421 gaaaagttgg ttttgcccac cttcaagacc gcgaaggcca gattcagatc tacgttcgta
      481 aggatgctgt cggtgaagaa aactacgaaa tcttcaaaaa agcagacctt ggtgacttcc
      541 ttggtgtcga aggtgaagtg atgcgtacgg atatgggaga actctctatc aaggcaaccc
      601 acatcacaca cttgtctaag gctcttcgtc ctcttcctga gaaattccat ggtttgacag
      661 atgttgaaac aatttaccgt aaacgttacc ttgacttgat ttctaatcgt gaaagctttg
      721 aacgctttgt cactcgttca aaaatcatct ctgaaatccg tcgttacctt gaccaaaaag
      781 gattccttga agtggaaaca cctgttcttc ataatgaagc cggtggtgct gctgcccgtc
      841 catttatcac ccaccacaat gcccaaaaca ttgacatggt gcttcgtatc gcgactgagc
      901 ttcacttaaa acgccttatc gtgggtggta tggaacgtgt ctatgaaatt ggccgtatct
      961 tccgtaacga aggaatggac gctactcata accctgagtt tacttctatc gaagtttacc
     1021 aagcttatgc agacttccaa gacatcatgg acttgacgga aggcattatc caacacgctg
     1081 ctaaatcagt taagggcgat ggcccagtca actaccaagg tactgaaatc aagattaacg
     1141 aaccatttaa gcgtgttcat atggtggatg ctatcagaga aattactggt gtcgatttct
     1201 ggcaagacat gactttagaa gaagctaaag ctatcgctgc tgagaagaaa gttccagttg
     1261 agaaacacta cactgaggtt ggtcacatca tcaatgcctt ctttgaagag tttgttgaag
     1321 aaactttaat ccaaccaacc tttgtctatg gacatccagt agctgtatct ccactcgcta
     1381 agaaaaatcc tgaagaccaa cgctttactg accgtttcga gctctttatc atgactaagg
     1441 agtacggtaa tgcctttact gaactcaacg acccaatcga ccaacttagc cgttttgaag
     1501 cccaagctaa agccaaagaa cttggtgatg atgaagcgac aggaatcgac tatgactaca
     1561 ttgaagctct tgaatacggt atgccaccaa caggtggttt gggaatcggt atcgaccgtc
     1621 tctgcatgct cctcactgat acaacaacta tccgtgatgt attgctcttc ccaacaatga
     1681 aataaattct tatcctctgg ctcttaccag aggatttttt gattcaaaaa gagactgaat
     1741 ttaaggagaa aatgaagtgt agtatattga agttgaaata gtacactttg atttctaaga
     1801 cattgttaga aattggttta aattccctaa gcaatttgtg catgttttat ttcattttac
     1861 gatagtacgc tgaaactgtt caaaaagtac tagaaattga cttggattcc ccaattgatt
     1921 tgttcagatt cactataaat aaaaaattaa taagtgggat aggaagttag cgtcaactag
     1981 gatagtatct tgcttaaaca gtatatatgg gattgatata agtccatagg tcctattaga
     2041 ggatgttctg gtgtcttatt cacttgtttt tttatagtat tagtagatag aatccgcaaa
     2101 taaaaaagta gttcaaatgc ctggcagcaa aaaatctcaa cgattcactt aatatagact
     2161 aaagtttaga gtttaaggaa agtagggcta gtatattgta tgaaaaaata agatggtttt
     2221 taagcaaaac tcttgaaaat gattgaaaaa agagttatta tagttgtgta gtaaaaagca
     2281 aacgcttact taatcaagga gggcatttta tgtcatacaa aacaagcaat gcagaaggtc
     2341 atgtagactt catcaatacc tatgatttgg agccaatggc gcaacaagtt attcctaaag
     2401 cagcatttgg ctatatcgct agtggggcgg aagatacttt cactttaaga gagaatatcc
     2461 gcgcctttaa ccacaaactc atcgttcctc atacactttg cgatgttgaa aatccaagta
     2521 cagagattga atttgcaggt gaaaaactat cttcaccaat cattatggcg cctgttgcgg
     2581 ctcataaatt ggcaaatgaa cagggggaag tggcgactgc gcgtggtgtg catgagtttg
     2641 gttctcttta tacaaccagc tcttactcta ctgttgacct tccagagatt tctgaagccc
     2701 ttcaagggac accccattgg ttccaatttt actttagtaa ggatgacggt atcaaccgcc
     2761 acatcatgga ccgtgtgaag gctgaaggtt ataaagcgat tgtcttgacg gcagatgcta
     2821 ctgtaggggg gaatcgtgaa gtggataagc gtaatggttt tgtcttccca gttggcatgc
     2881 cgattgttga agaatacctg ccagaaggtg ctggtaaatc aatggacttt gtttacaaat
     2941 cagctaaaca acgcttgtct ccacgcgatg tagaatttat cgctgaatac tctggtcttc
     3001 ctgtgtatgt caagggacca caatgccgtg aggacgttga acgttcactt gctgcaggag
     3061 cttctggtat ctgggtaacc aaccacggtg gtcgtcaaat cgacggtgga ccagctgcct
     3121 ttgactcgct tcaagaagtg gcagaagcag ttgatagacg tgtgccgatt gtctttgact
     3181 ctggtgttcg tcgtggtcaa cacgtcttta aagccttggc ttcaggagca gacttggtag
     3241 ctattggtcg ccctgttatt tatggcttgg ctctcggtgg tagtgtcggt gtgcgtcaag
     3301 tctttgaaca cttgaatgcg gaattgaaga cagttatgca attgtctgga gctcagacca
     3361 ttgaagatgt caaacacttt aagctccgtc acaatccata caacccaacc ttcccagttg
     3421 accctcgtga cttaaaattg tattgataaa acagattgcc ttcactgaat gtggaggttt
     3481 ttcttcgtgt attggaagaa taaatcgcta tcgaaaacta tagtaaaatg aaataagaat
     3541 aggacaaatc aatcaggaca gttaaatcga tttctaacaa tgttttagaa gtagaggggt
     3601 actattctag tttcaatcta ctatatataa agatattttt cggttaaata tcttgctaag
     3661 gctttcattt tttgctatac tggttcagta attaaataat agagacattt ggggtgcttt
     3721 aggctgagat aatacccatt gaacctgata cagttaagac tggcgaaggg aaatgtgaac
     3781 agttttttcg tatggtgaaa tgaacggtct aatcttgtaa gtcaactcta attcttgatt
     3841 gtaattggag ttttttgttt atataaactg gataggcttg tttagttagc tctctgcaaa
     3901 tactctcgat gtgttttaaa aggatcaagt ggaattacga ttttgcttga tatttcttgg
     3961 tgttttatta attactaaca agtaaagaga ggagaagtaa atatggaaac acaagactat
     4021 gcatttcagc caggtttgac tgttggagaa ttattaaaaa gcagtcagaa ggattggcag
     4081 gctgcaatca atcatcgttt tgttaaggaa ctttttgcgg ggacaattga gaataaggtc
     4141 ttaaaggact atctgattca agattatcac ttctttgatg ccttcttatc catgctgggt
     4201 gcctgcgtag ctcacgcaga taaacttgaa tccaagcttc gttttgccaa gcaactaggc
     4261 tttcttgaag cagatgaaga tggttatttc caaaaggctt ttaaagagtt aaaagtagca
     4321 gagaatgact atctagaagt gactttgcac cctgtaacaa aagcctttca ggatctaatg
     4381 tattcggcgg tggcttcatc agactatgcc catcttttgg tcatgctggt cattgcagaa
     4441 ggtctctatt tagactgggg ttctaaagat ttggctttac ctgaagtcta tattcattcg
     4501 gaatggatca atctccacag aggtcctttc tttgcagagt gggttcaatt tctggttgac
     4561 gaactcaatc gtgtcggtaa aaatcgagaa gatttgacag aacttcagca acgctggaat
     4621 caagcagtcg ctttagaatt agcttttttt gatattggtt acgatgtcta gagaaggctc
     4681 atgatattta caaatttgat gaggtaatac tcttcgaaaa tctcttcaaa ccacgtcagt
     4741 ttcaccttgc cgtaggtatg gttactgact tcgtcagttc tatccacaac ctcaaaacag
     4801 tgttttgagc tgacttcgtc agtcttatct acaggttcaa agcagtgttt tgagcaatct
     4861 gcggttagct tcctagtttg ctctttgatt ttcattgagt ataaatcatt caacgtaaat
     4921 gaaaaaaatt tcttaacgaa agatagagat aaatcgaaat cagtagatcg tataaagtga
     4981 aaaggaagga tttcaatatg acaagtttaa aattattaaa agaaaaagca ccattggtca
     5041 tttgtataac caatgatgta gtaaaaaatt tcacagcaaa tggattagta gcactgggcg
     5101 tctcaccagc catgagtgag tttccagcag atttagagga tttgttaaag tatgctggtg
     5161 gtttattaat aaacatagga acattgacag atgaaaattg gaaattatac caagctgctc
     5221 tgaaaattgc ggagaaatat aatgtcccag cagttttaga tcctgtagcc tgtggagcag
     5281 gagaatatag aaaaaaagta gcagatgatc taatcaacaa ttataaacta gcagcgatta
     5341 gaggaaatgc tggcgagatt gcctctttag taggaataga tgtggcatct aaaggagtag
     5401 atagtgcggg cgtagataat attgacgaaa ttgctctagc agcaaatgag aagttcaata
     5461 ttccaatagt agtaacaggt gaagtggatg ccattgctgt taatggagaa gtggtaacga
     5521 ttcataatgg tagtgctatg atgccgaaag tcattgggac aggatgctta ttaggagctg
     5581 tagtagcaag ctttatcgga ctagaaaaag gtcaagaatt gaaatcatta gaaacagcaa
     5641 tgttagttta caatatcgct ggagaaatgg cagaaaaacg tccaaatgga catcttcctg
     5701 ggacatttaa agttgaattt ataaatgcct tatacgagat tacagatgaa gatgtaaagg
     5761 aattcaaaag agtgaagtaa gatgtttcat aaagaattac taaaactata ttttatttgt
     5821 ggaacgacta cttgccaagg aaaaaatcta tatacagtcg ttgaggaagc cttaaaaggt
     5881 ggtataacct tatttcaatt tcgagaaaaa ggtgagagtg ctttagaagg cttagaaaaa
     5941 cttgagctag ctatccaaat taaagaactt tgtaaaaaat acaatgttcc atttatcgtt
     6001 aatgacgata tagatttagc aatggaaatt gacgccgatg gcgtacatgt tggtcaagat
     6061 gatattggtg ttgatgaaat tagaaaattg atgccagata aaataattgg tctttctata
     6121 agaaacgaag aagaatttca acaatcaaaa gttgaatatg tagattatgt aggtgttggg
     6181 cctgtatttg atacccagtc aaaagatgat gctggtggtg ctataggtta tgaaggtctt
     6241 gaattgatga gaaaactatt gccacaaatg cctttagttg caattggtgg gatacagacg
     6301 aaacatatta aagacattat aaagaccaat gttgacggcg tttcaatcat ttcggcaata
     6361 tcgtatgcaa aaaatataga aaaaactgtt cgggaaatga gtgaacaata gatatctatc
     6421 cttggattct aagtaagtag gattttcctt ttttaaaaag ctatgtctag attcagcttg
     6481 aatggctaat tcatcaaaca ggtcatttgt tttttgcaaa agaaagatct gctgtctttg
     6541 tgagtgcaag gctgtatctc cagttcaatt tcttgaacta gtataaaatg tgtgttataa
     6601 taatcacata attaaataat aaaggcattt ggggtgcttt aggctgagat gatacccatt
     6661 gaacctgata cagttaagac tggcgaaggg aaatgtgaaa tgtttttttc gtatgtcgaa
     6721 aagaactgtc taatcttgta agctgaactc taattcgtat ctgtaattag agttttttct
     6781 tttacgaaac tggatgaaga tgatatagta gattgaaata agatgcgaac aaatcaatta
     6841 gaaaattcaa attaatttct agaaatattt tagcattcct gtgtactgtt ctaaattcaa
     6901 tctgctatac aaagtattta ggtcggtctt cttgagtgcc ccacttttct tttaaaaaag
     6961 gagtttttta tgttgaaaaa atggcagtta aaagatgtta tcttgcttgc tttcttgtct
     7021 atcttttttg gtggggtttt cgttggttca ggatatgtgt ataatattct cagtctactc
     7081 ttaacacctc ttggtttgca ggcctttgcc aatgaaatcc tcttcggtct ctggtgtatg
     7141 gctgcgccca ttgctgccat ctttgttccg agagtcggaa gtgcaacgat tggagaagtg
     7201 ctagctgcgc ttgctgaagt cctttatggt agccaatttg gtctaggagc tcttttgtct
     7261 ggcttggttc aaggtttggg aagtgaattt ggttttatcg taactaagaa tcgctatgaa
     7321 agttggctct ctctaactgc taatagtatt ggggttacgc ttgttagctt tgtctatgaa
     7381 tacattaagt taggttacta cgccttttcc cttccgtttg tcctttcctt acttgtggta
     7441 cgttttattt ctgtttattt cttctgtacc atcttggttc gtgccattgt caaactctat
     7501 catcagtttg caactggagg aaaagcatag atgggtctgg aactacgagc gattcagtcc
     7561 ccaatcttct ctgagccgtt tgattttact tttcatgcgc aagcctttac cttgttagtt
     7621 gggagcagtg gttcaggaaa atccagcctt tttcaagtca ttgcccaagt cagttctctt
     7681 ccctatagtg gtcaagtcct gatagatggg agcgaggtca gtcagctttc tatcatcgca
     7741 cgtgtccaga aggttggcat tctctttcaa aatcccaatc atcaatttac catggagaac
     7801 ttgtttgagg agctgatttt caccttggag aatatcggct atcaccttca ggaaattgat
     7861 tctaaaatag cagaggttgt ccagcaatgt cgttgcgagg caatcttaca ccgtcccatt
     7921 catcacctat caggtgggga aaagcaaaaa gcagcgctgg ctgtcctctt tgccatgaat
     7981 cctagggtct atctcttgga tgagcccttc gcttctattg accgcaagag cagaatcgag
     8041 atattggaga ttctaaaaga gttggctctt gatgggaaga cagttatttt gtgcgaccat
     8101 gatttatctg actataaagc ctatatcgac catatggttg agctaagaga cggaaaacta
     8161 agggaagtgt ttcaaatccc ttcctatgag atgacacagg ttgcttcaaa ggaagttgct
     8221 tctagcccgg aactattcca tatgaaccgt gtgactggtg agcttggtaa tcgccccctc
     8281 ttttcaattg ctgatttcac attctatcaa gggatttcct gtatcctggg tgacaatggt
     8341 gtcgggaaat caaccctctt tcgctctatt cttcaatttc aaaagtataa ggggcgcatt
     8401 gcatggaagg ggacagtcct gaaaaagaaa aagagtttgt accgtgatct gacgggtgtt
     8461 gttcaggaag ctgagaagca gtttatccga gtcagtctgc gagaggagct tcgattagat
     8521 ggacctgatt ctgaaagaaa tcagcggatt tttcaagctt tacgatattt tgatttggag
     8581 caggcagtcg ataagagtcc ctatcaatta agtggtggtc agcaaaaaat tcttcagctc
     8641 ctgaccatct tgaccagtaa ggcttccgtg atcttgctag atgaaccttt tgcaggtttg
     8701 gatgatagag cctgccatta tttttgcaag tggattgtgg aggagaggaa tcaaggaaga
     8761 agttttctgc tcattagtca tcgtttagac cctttgattt ctgtggttga ttattggatt
     8821 gagatgacta gtcaggggct cagtcatgtg aaagaagtga ccattaccaa accacttaca
     8881 tctcagagta gcaataccca aggggaggtg agatagtatg gtcaaagtag caacccagac
     8941 accgattatc agtctcttct tgctgatttt atccttggaa acatctttca ttccttcgat
     9001 tgctctgaat ctttcggtag tcgcattttg tattctcttt atgctctatt accgtcgatt
     9061 taaaatgtta gcttggatga tcatacttgc cattttacca tcttttgcca actactgggc
     9121 agttcagtta cacggagatg cttcacaggc agtcatgctt ggaacgaggg cctttgtgac
     9181 agtttgtatc ggccttgtct ttgtttcctc tatttcacta aaagagcttc tcttgtactt
     9241 ggctcaaaag gggctatcac gctcttggtc ctatgccttg attgtggtat tcaattcctt
     9301 tcctctcatt cagcaagaaa tcaagtccct caaagaagct tgcctattac gtggtcaaga
     9361 actgcatttt tggtcgccct tgatttacag taaggttctg atgacagtct ttaggtggcg
     9421 ccatctttac ctgagagctc tgtctgctca tggatatgac gaacatgcac agttgaagaa
     9481 tagctatcgg actttttata ttcctaaaaa aacaaaatta atctacctgc ttttcttttt
     9541 attgcttcaa accagtctat ttttataaag gagttattat ggaatttaca gatattgcga
     9601 tggaattatc caagaaagct tggcaggctt cctttcatca tccctttatt ttacaattac
     9661 aagaggggaa tttagaacct gccattttcc gctattacct gattcaggat gcctactatc
     9721 tgaaggcctt ctcagaaatc tatcatcttt tggctgataa gacttcaaac caagagatga
     9781 aaagactctt gaaacaaaat gctcagggtt tagtggaggg tgagttattt attcgccaac
     9841 aatttttcaa ggaaatggaa atcagcgacc aggaaatgga gcaacatcca attgctccaa
     9901 cctgttatca ttacatttct catatttata ggcaatttgc agaaccgaac ttagccatcg
     9961 cttttgcaag cttgcttcct tgtccttggt tataccatga tataggcaaa tcacttaatc
    10021 ttaaaccatc accaaatcct ctctatcaac aatggattga aacttatatt acggatgagt
    10081 tagagcaaca gatcagagag gagggagcac tggtcaatca gctctatcga gaaagtgatg
    10141 agacagataa gcaaaaaatg ctagatgcct tccacatcag tgttcatatg gaagccaagt
    10201 tttgggagat ggcttaccaa caccagacat ggaagagcga tttacagtct ttagaaaaag
    10261 gagaagaata gaaacatgag aaagcaccaa ctacaagttc acaaattgac cattttatct
    10321 atgatgattg cccttgatgt agtccttaca cctatctttc ggattgaggg aatggcaccg
    10381 atgtccagtg tagtcaatat tctagcggga atcatgatgg gacctgttta tgccttggct
    10441 atggctacag tgacagcctt tatccgtatg acgactcaag ggattccgcc tttagctctc
    10501 acaggagcga cttttggagc ccttctagca ggtctctttt ataagtacgg tcgaaaattt
    10561 tacttttctg ccttgggaga aattgtggga acaggtatta ttggttctat tgtttcctat
    10621 cctgttatgg tgctctttac aggatcggct gctaagctta gttggtttat ctacacgcct
    10681 cgatttttcg gagcaacctt gattggtaca gcgatttcct ttattgcatt tagattttta
    10741 atcaagcagg aattctttaa aaaagtgcaa ggatatttct ttgctgaaag gatagaatga
    10801 tgcaggcatt gacaaatcct tttcctatag gctctagttc cctcattcac tgtatgacca
    10861 atgagatttc ttgtgagatg ctggcaaatg ggattttggc tctgggatgc aaacctgtca
    10921 tggcagatga tccccgtgaa gttcttgatt ttactaagca aagccaggct ctcttcatca
    10981 atttggggca tttgtcagct gagaaggaaa aagcaatccg catggcagct ttgtatgcaa
    11041 accaatcttc tctcccgatg gtagtagatg cggttggcgt aacgacttca tccattcgta
    11101 agagcttagt taaagacctt ttagactata gacctacggt ccttaaagga aacatgtcag
    11161 aaattcgaag tcttgttgga ttaaagcatc acggcgttgg ggtcgatgcg agtgctaaag
    11221 atcaagaaac ggaggatttg cttcaagtct tgaaagactg gtgtcagacc tatcctggta
    11281 tgccattctt agtcacaggt tccaaggacc ttattgtttc ggagaatcaa gttgctgtac
    11341 ttgagaatgg ctgtactgaa ttagactgga taacagggac aggagacttg gttggagcct
    11401 taacagctgt ttttctcagc caaggaaaga ctggttttga agcttcttgc ttagcagtct
    11461 cttatctcaa tatcgctgct gagaaaatag ttgttcaagg aatgggattg gaagaatttc
    11521 gttaccaagt actcaatcag ctttcgctcc taagaagaga tgaaaattgg ctagatacca
    11581 tcaaaggaga ggtttatgaa tagagaagcg cttagactat atctggtaac caatcgctat
    11641 caagattccg tggaaagctt tcttgcaaaa gttgagacgg cctgccgttc aggggttacc
    11701 atagtccaat tgcgagaaaa aaatctcaca accaatcaat attatcaact ggcaaaacaa
    11761 gtcaaggaaa taacagatgc ttatcaggta cccttgataa tcgatgatcg gttggatgtt
    11821 tgtcttgcgg ttgatgctgc tggtctgcat attggagatg acgaactccc agtttcggtt
    11881 gcccgacaag tcttgggtcc tgaaaaaatc ctcggtgtca ccgctaaaac ggttaaaaga
    11941 gctctggaag cggagaaatc aggtgcagat tacttgggga caggagccat ttttccgaca
    12001 accaccaagg aaaatgcacc catcaccctg atttcaacct tgaaaacaat ttgccaaacg
    12061 gttgccattc caatagttgc tattggcggc ttgacttcgg agaatattga tcagcttatg
    12121 ggcactggca tagctggtgt agctgttgta cgtgatttga tgcaagcaga agatattgag
    12181 gcaaaaacgc aagccttttt aacaaagttg catgacattc tttcctaatt aatactcaat
    12241 gaaaatcaaa gagtaaacta gaaagctagt cgcaggttgc tcaaagtact gccttgaggt
    12301 tttagataag actgacgaag tcagctcaaa acagtgtttt gaagttgcag atggaagctg
    12361 acgtagtttg aagagatttt cgaagagtat aaaaactctc taattccctt aaagtgggaa
    12421 ctagagagtt tttttcttaa tctttaaaag ttgtatggtt gactggacca gaaccatgac
    12481 cgagttgagg ggcatcttga atagcttttg tgataaaggc cttggcctta tcaactgcct
    12541 ggtaaagact cttgcccttg gctagttcag cagtaatcac tgcagcaaag gtacatccag
    12601 taccatgggt gtgacaggtt tgaattcgtg ggctttccca gacaaattgt tcattcttgg
    12661 taaagaggaa atctttagca ccacctttga gatgtccgcc tttgagatgt ccgcctttga
    12721 taaccacaga ctgaggacca aattctttta aaatcaggcg accagcacgc tgcatgtctt
    12781 cggggtcatg gattgaaaaa ccaacaatct cttctgcttc aggaagattt ggcgtaataa
    12841 tagttgctag aggtagtaag tttgttttga gatagtctct agcatttgag tcaatcaagg
    12901 catctccact tgtagcaacc ataacaggat caaggacata gggacaatcc agttttttaa
    12961 gatagggttg gatgatttcc atgatttcag tagtagccaa cattccagtt tttacagcct
    13021 gaggtggaat atcagaaaag acactctcca attgggcttt caacatttga ggagaaacgt
    13081 gctcgattag ctgaacacct ctggtatttt gagcgacaag actggttaca acagccattc
    13141 catagacatc tctcgcttgg aatgacttta aatctgccat aatgccagca ccaccactag
    13201 ggtcagtccc tgcaatggtc aaagcaacgg gtaaataagt catctaaaac ctcccaacca
    13261 actgaagttt gtattcttaa gaacaagcta gtctgtttta gaaaggcaat agaagactgc
    13321 tagtccccat tatcatttcc acttccatca gctagcatta cctagattga gtcagaggga
    13381 tctaacagtc gttaatctca gctttacgca cccctagtgg ttgttttctt ttttctttag
    13441 tatagcatat ttttattgtt tatatagtaa tatttgactg caaatcgttt ttcaaatgat
    13501 taaaattcta tttttcgaag aaaatattat ttctataatt gacaaatgta gattttaaga
    13561 gtatactgat gagtgtaatt gacaaatgta gattttggag gtatggtaat gcagatttca
    13621 gatgcagaat ggcaggtcat gaagattatt tggatgcagg gggagcagac cagtacagat
    13681 ttgattaggg ttttggcaga gcggttcgac tggtccaagt caaccattca aactcttttg
    13741 gctcgtttgg ttgagaaaga gtgtctgact cggaaaaaag aaggcaagtt ctttgtttat
    13801 tcagcccttt taactctaga ccaaagtcgg gatttacttg tccaagatat caaagacaag
    13861 gtttgttccc gtaggattag gaatttgttg gctgacttga ttgtagaatg tgaatttact
    13921 cagactgact tggaagactt ggaagctgtg atttcagaga agaaatcaag tgctgtaaca
    13981 gaagtaagat gtaattgtat gtaaaggaga cgtcatgtta aatagtattg taaccattat
    14041 ttgtattgcc cttatcgcgt ttatcttgtt ttggtttttc aaaaagcctg aaaaatctgg
    14101 acaaaaagcc cagcaaaaaa acggatacca agagattcga gtggaagtca tgggaggcta
    14161 tactcctgag ttgattgtcc tcaagaaatc agtgccagcc cgcattgtct ttgaccgcaa
    14221 ggatccttca ccatgtctgg atcaaattgt ttttccagat tttggtgtac atgcgaacct
    14281 gccaatgggg gaagagtatg tagtggaaat cacgcctgaa caggctggag agtttggctt
    14341 tgcttgtggt atgaacatga tgcacggcaa gatgattgta gagtaggtgg agactatgac
    14401 agaaattgtg aaagcaagct tagaaaatgg cattcaaaaa atccgtatcc gagctgaaaa
    14461 aggctatcat ccagcccata tccagcttca aaagggaatt ccagctgaga ttacctttca
    14521 tcgtgctact ccttcaaact gttataagga aattctgttt gaagaagaag gtatcttgga
    14581 accaatcggc gtagatgagg agaaagtcat tcgttttaca cctcaagaat tagggcgaca
    14641 tgaattttct tgtggcatga agatgcaaaa gggaagctat atagtcgttg agaagactcg
    14701 aaaatctcta tctctcctgc aacgtttttg gattactagt atctttactg tgcctcttgt
    14761 gattctcatg attgggatgt tgacaggtag cattagtcat caagtcatgc attggggaac
    14821 ctttctagca acaacgccta ttatgttagt tgcaggtaag ccatatatcc agagtgcttg
    14881 ggccagtttt aaaaagcaca atgccaacat ggataccttg gttgcgctgg gaactctagt
    14941 ggcttatttc tatagcctag ttgctctctt tgctggtctc cctgtttact tcgaaagtgc
    15001 tggatttatc ctctttttcg ttcttttggg agcagttttt gaggaaaaaa tgagaaaaaa
    15061 tacgtcccaa gctgtggaga aattactgga cttgcaagct aaaaccgcag aagtcttgag
    15121 tgatgatagt tatgtccaag ttcctttgga acaagtcaag gtaggcgacc tgattcgagt
    15181 gcgtcccggt gaaaagattg ctgttgatgg tgtcgtagta gaaggtgtct ctagtattga
    15241 cgaatccatg gtgacaggtg agagtctgcc tgtggacaag acagttggag atactgtcat
    15301 tggctcaacc atcaatcata gtggaacgct tgtctttaga gcagaaaaag ttggctcaga
    15361 gactgttttg gctcagattg tggattttgt gaagaaagct cagacaagtc gtgcgccgat
    15421 tcaggacttg acggataaga tttcagggat ttttgtccca gtagttgtca ttttaggaat
    15481 catgaccttt tgggtttggt tcgtcttgct cagggatagt gtggtcgtgc ttggagctag
    15541 ctttgtgtcc tctcttctct acggagtggc ggttttgatt atcgcctgtc cttgtgcctt
    15601 gggacttgca acaccgacag cccttatggt ggggacagga cgtagtgcca agatgggggt
    15661 tctcctcaaa aatggaactg tcttacagga aatccagaaa gttcaaactc ttgtctttga
    15721 taagaccggg actttgacgg aagggaaacc tgtggtaaca gatataatcg gcgacgaagt
    15781 agaagtgttt ggattggcag cctccttgga agatgcttct caacacccac tggctgaggc
    15841 tatcgttaag cgagcgagtg aagctggact tgagtttaaa actgttgaaa attttcaggc
    15901 cttgcacggg aaaggtgttt cagggcgaat caatggaaaa caagttttac ttggaaatgc
    15961 taaaatgctg gatggcatgg atatttctaa tacttatcaa gataaactag aagaactaga
    16021 aaaagaagct aagacagttg tgttcttagc tgttgacaat gaaatcaaag gcttgcttgc
    16081 tttgcaagat attcctaagg aaaatgctaa gctagccatc agtcagctaa aaaaacgtgg
    16141 tctccgaaca gtcatgctga caggagacaa tgctggtgtg gcgtgtgcta ttgcagatca
    16201 aatcggaatt gaagaggtca ttgcaggcgt cttgccagaa gaaaaagccc atgaaatcca
    16261 taaactgcaa cagtcaggga aagtagcctt tgttggggac ggtatcaatg acgctcctgc
    16321 ccttagtgta gcagatgtgg ggattgctat gggagctgga acagatatcg ccatcgagtc
    16381 agcagatttg gtgttgacaa ccaataatct tttaggagtg gttcgtgcct ttgatatgag
    16441 taagaaaacc tttcatcgaa ttctactcaa tcttttctgg gcttttatct acaatgttgt
    16501 cggaattccg attgcagcag gagtcttttc aggtgttgga ctggctctca acccagaatt
    16561 ggcaggtcta gccatggcct ttagttctgt atctgttctg accagttccc ttctgttaaa
    16621 ttttagtaaa atagactaaa agtaatgact agatttcttt gttataaaac agaaatgaca
    16681 gaactttcaa aactgtatta gaatagagaa tagagagttt tgagcagatt tttagaaaag
    16741 tcagcataaa tatgatacag tggaatagta aaaatttgga gaacgtttcc aattctatgt
    16801 aatcgtattc tccaaatcta aaaaaattga aggagagtta tcattatgac tcaagggaaa
    16861 attactgcat ctgcagcaat gcttaacgta ttgaaaacat ggggcgtaga tacaatctac
    16921 ggtatcccat caggaacact cagctcattg atggacgctt tggctgaaga caaagatatc
    16981 cgcttcttac aagttcgcca cgaagagaca ggtgctcttg cagcggttat gcaagctaaa
    17041 ttcggcggct caatcggggt tgcagttggt tcaggtggtc caggtgcgac tcacttgatt
    17101 aacggtgttt acgatgcagc tatggataac actccattcc tagcgatcct tggatcacgt
    17161 ccagttaacg aattgaacat ggatgctttc caagagctta accaaaaccc aatgtacaac
    17221 ggtatcgctg tttacaacaa acgtgtagct tacgctgagc aattgccaaa agtaattgac
    17281 gaagcctgcc gtgctgcaat ttctaaaaaa ggtccagctg ttgttgaaat tccagtaaac
    17341 ttcggtttcc aagaaatcga tgaaaactca tactacggtt caggttcata cgaacgctca
    17401 ttcatcgctc ctgctttgaa cgaagttgaa atcgacaaag ctgttgaaat cttgaacaat
    17461 gctgaacgcc cagttatcta tgctggattt ggtggtgtta aagctggtga agtgattact
    17521 gaattgtcac gtaaaatcaa agcaccaatc atcacaactg gtaaaaactt tgaagctttc
    17581 gaatggaact atgaaggttt gacaggttct gcttaccgtg ttggttggaa accagccaac
    17641 gaagtggtct ttgaagcaga cacagttctt ttccttggtt caaacttccc atttgctgaa
    17701 gtttacgaag cattcaagaa cactgaaaaa ttcatccaag tcgatatcga cccttacaaa
    17761 cttggtaaac gtcatgccct tgacgcttca atccttggtg atgctggtca agcagctaaa
    17821 gctatccttg acaaagtaaa cccagttgaa tcaactccat ggtggcgtgc aaacgttaag
    17881 aacaaccaaa actggcgtga ttacatgaac aaactcgaag gtaaaactga gggtgaattg
    17941 caattgtatc aagtttacaa tgcaatcaac aaacatgctg atcaagacgc tatctactca
    18001 atcgacgtag gtaacactac tcaaacatct actcgtcacc ttcacatgac acctaagaac
    18061 atgtggcgta catctccact ctttgcgaca atgggtattg cccttcctgg tggtatcgct
    18121 gctaagaaag acaatccaga tcgccaagta tggaacatca tgggtgacgg agcattcaac
    18181 atgtgctacc cagacgttat cacaaacgtt caatacgacc ttccagttat caaccttgtc
    18241 ttctcaaatg ctgagtacgg cttcatcaag aacaaatacg aagatacaaa caaacacttg
    18301 tttggtgtag acttcacaaa cgctgactac gctaaaattg cggaagctca aggagctgtt
    18361 ggattcacag ttgaccgtat cgaagacatc gatgcagttg ttgcagaagc tgttaaattg
    18421 aacaaagaag gtaaaactgt tgtcatcgat gctcgcatca ctcaacaccg tccacttcca
    18481 gtagaagtac ttgaattgga tccaaaactt cactcagaag aagctatcaa agccttcaag
    18541 gaaaaatacg aagcagaaga actcgtacca ttccgtctct tcttggaaga agaaggattg
    18601 caatcacgcg caattaaata attcctctcg ccgaaaatca aatatgaaac ttgtagactt
    18661 tattctttga ttttcataga gcagaacttt aaaagatcgg agcaatccgg tctttttatg
    18721 gaggagagta aatgaattta aatcaattag atattatcgt ttcaaatgtt ccccaagtct
    18781 gtgctgactt ggagcatatt ttggataaaa aggcagatta tgctgatgat ggttttgctc
    18841 agtttacgat tggtagtcac tgccttatgt tgtcacaaaa tcatttggtt cctttggaaa
    18901 actttcagtc aggaatcatt attcatatcg aggttgagga tgtagaccag aactacaaac
    18961 ggttgaacga gcttggtata aaggttttac acggaccaac tgtaaccgat tggggaacag
    19021 agtccttatt agttcaagga cctgctggtc tagtgcttga tttttatcgt atgaaatagg
    19081 tcgcgtagtc atttgacgtt aaccttagct atttttaaaa tagaaattga gaatatgaac
    19141 tattgaaaga tcggagcaat ccggtctttt tatataaaga aattgcatat aatactcatc
    19201 tgctttcaaa aagcattcta gtccatctcc gattaacgat ggactttatc acctccttct
    19261 ccaatccttg tatgacatct tggagttgat tcatgacatc ttccaaaatt cgaaaggctt
    19321 tattcttaaa tccacgttta cgaatctctt tccacacttg ttcaatgggg ttcatctctg
    19381 gtgtgtatgg aggaataaat gcaaaaccaa tattagtcgg aatctttaag atacttgatt
    19441 tatgccatat agcattgtcc ataacgagta aaagataatc atctggataa gcttgtgaaa
    19501 gctcttctaa aaaggcgttc atccactcag tattacatct accagctatt aagaaaaatg
    19561 attcgcctgt ataggcatca acagctccat aacaatagcg aaattctcgt atatagtgac
    19621 tatggacatg tggatctact cctattggag accaacaaga tcccagttta ctgattctac
    19681 tgaaaccagc ctcatcttgg tacatcaagc gaaccttacg aaaacgtcta ctatatttaa
    19741 agcgctttct tgccttcttg aattgagatt ttatttttag acgcgacaat ggtttgagcg
    19801 tctgctttcc taggatgttc tggacgtggc ataatatttc gccaaccatg gtgcttcaac
    19861 agttgataga aggcatcacg tgtgtaggaa cgacctaact cctttttata agcctgaaat
    19921 aaggcatcaa ttgtaacaaa ttctcctgcc tctgtagcct tcaaatggcg agcaagaaag
    19981 gctttctctt cctcaactgt catatatgca tggttacgac caccacgtgt ttcttgaagg
    20041 agagagtcga gtccgaactc ctcatatttt ttacatttcg c
//
LOCUS       DAVLVP010000028        19884 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.028, whole genome shotgun sequence.
ACCESSION   DAVLVP010000028 DAVLVP010000000
VERSION     DAVLVP010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19884)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19884)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19884
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.028"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(95..1726)
                     /gene="rlmD"
                     /locus_tag="VYN69_000966"
     CDS             complement(95..1726)
                     /gene="rlmD"
                     /locus_tag="VYN69_000966"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000914584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1533258.1"
                     /translation="MLKKNDIVEVEIVDLTHEGAGVAKVDGLVFFVENALPSEKILMR
                     VLKVNKKIGFGKVEKYLVQSPHRNQDLDLAYLRSGIADLGHLSYPEQLKFKTKQVKNS
                     LYKIAGIADVEVAETLGMEHPVKYRNKAQVPVRRVNGVLETGFFRKNSHNLMPLEDFF
                     IQDPVIDQVVVALRDLLRRFDLKPYDEKEQSGLIRNLVVRRGHYSGQIMVVLVATRPK
                     VFRVDQLIEQVIKQFPEIVSVMQNINDQNTNAIFGKEWRTLYGQDYITDQMLGNDFQI
                     AGPAFYQVNTEMAEKLYQTAIDFAELKKDDVVIDAYSGIGTIGLSVAKHVKEVYGVEL
                     IPEAVENSKKNAQLNNISNAHYVCDTAENAMKNWLKDGIQPTVILVDPPRKGLTESFI
                     KASAQTGADRIAYISCNVATMARDIKLYQELGYELKKVQPVDLFPQTHHVETVALLSK
                     LDVDKHISVEIELDEMNLTSAESKATYAQIKEYVWNKFELKVSTLYIAQIKKKCGIEL
                     REHYNKSKKDKQIIPQCTPEKEEAIMDALRHFKMI"
     gene            complement(1800..2774)
                     /locus_tag="VYN69_000967"
     CDS             complement(1800..2774)
                     /locus_tag="VYN69_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1002 domain-containing protein"
                     /protein_id="HEV1533259.1"
                     /translation="MRKKLFLTSAAVLWAVTAMNSVHAATDVQKVIDETYVQPEYVLG
                     SSLSEDQKNQTLKKLGYNASTDTKELKTMTPDVYSKIMNVANDSSLQLYSSAKIQKLG
                     DKSPLEVKIETPENITKVTQDMYRNAAVTLGVEHAKITVAAPIPVTGESALAGIYYSL
                     EANGAKVPQANKDLAQEELKALSDINAENKDKSGYDANKLNVALADIKSGLAKAKESK
                     GNLTEEDIRKIVEDTLKNYKLDQVITGNQINIIINFALNLSKSDILSNADFTKTLNDL
                     KQSIVSQAGDSFKNINLNFDADKALEDGGNFLSSLWQALVNFFKSFGS"
     gene            complement(2848..3462)
                     /gene="pmp23"
                     /locus_tag="VYN69_000968"
     CDS             complement(2848..3462)
                     /gene="pmp23"
                     /locus_tag="VYN69_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase Pmp23"
                     /protein_id="HEV1533260.1"
                     /translation="MFKRIRRVLVLAVFLFAGYKAYRVHQDVKQVMTYQPMVREILSE
                     QDTPANEELVLAMIYTETKGKEGDVMQSSESASGSTNTINDNASSIRQGIQTLTGNLY
                     LAQKKGVDIWTAVQAYNFGPAYIDFIAQNGKENTLALAKQYSRETVAPLLGNTTGKTY
                     SYIHPISIFHGAELYVNGGNYYYSRQVRLNLYIIKCFTLFSTSG"
     gene            complement(3462..4436)
                     /locus_tag="VYN69_000969"
     CDS             complement(3462..4436)
                     /locus_tag="VYN69_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEV1533261.1"
                     /translation="MDIYIKKAIIHQFSPDDTELFLADKFLNITPKIEEYLRKKIEHV
                     YSDEAKTGIFEEENPFFNHITDDLLETSVTLANLWKEEFSISENLKTNDLIFVQFSKE
                     GVEHFAFLRIALRETLTHLGGEVDNPIKLTQNNLPGFGTGADEALVVNLQSRKYHLIE
                     KRIKYNGTFLNYFSDNLLAVAPKISPKKSIKELEKTAQRIAESFNTDDFQFQSKVKSA
                     IFNNLEESNELSPEKLANDLFDNNLTARLSFIDQVKEAVPEPVQFDEIDASRQLKKFE
                     NQKLSLSNGIELIVPNNVYQDAESVEFIQNENGTYSILIKNIEDIQSK"
     gene            complement(4444..5700)
                     /locus_tag="VYN69_000970"
     CDS             complement(4444..5700)
                     /locus_tag="VYN69_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEV1533262.1"
                     /translation="MIFDKDDFKAYDADLWNAIAKEEERQQNNIELIASENVVSKAVM
                     AAQGSILTNKYAEGYPGRRYYGGTDVVDVVESLAIERAKEIFGAKFANVQPHSGSQAN
                     CAAYMALIEPGDTVMGMDLAAGGHLTHGAPVSFSGQTYNFVSYSVDPETELLDFDAIL
                     KQAQEVKPKLIVAGASAYSQIIDFSKFREIADAVGAKLMVDMAHIAGLVAAGLHPSPV
                     PYAHITTTTTHKTLRGPRGGLILTNDEELAKKINSAIFPGIQGGPLEHVVAAKAVSFK
                     EVLDPAFKEYAANVIKNSKAMADVFLQDPDFRIISGGTENHLFLVDVTKVVENGKVAQ
                     NLLDEVNITLNKNSIPYETLSPFKTSGIRIGAAAITAREFGEEESRKVAELIIKTLKN
                     SENEAVLEEVRSAVKELTDAFPLYEE"
     gene            complement(5761..6192)
                     /locus_tag="VYN69_000971"
     CDS             complement(5761..6192)
                     /locus_tag="VYN69_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000942930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1533263.1"
                     /translation="MLRDLQETDVKAICDINQEALGYSFSPEDTASQLARLSQDSHHF
                     LLGYEDAANHVLLGYVHAEVYESLYSKAGFNILALAVSPQAQGQGIGKSLLQGLEQEA
                     KRCGYGFIRLNSANHRLGAHAFYEKVGYTCDKMQKRFIRIF"
     gene            complement(6194..6793)
                     /locus_tag="VYN69_000972"
     CDS             complement(6194..6793)
                     /locus_tag="VYN69_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEV1533264.1"
                     /translation="MDRIRQELENGGAVILPTETVYGLFAKALDEKAVDHVYQLKRRP
                     RDKALNLNVASLEDILHFSKNQPAYLQKLVETFLPGPLTIILEANDRVPYWVNFDLAT
                     IGFRMPSHPITLDLIRETGPLIGPSANISGQASGVTFEQILKDFDQEVLGLEDDAFLT
                     GQDSTIVDLSGDKVKILRQGAIKREDILARLPEISFEEE"
     gene            complement(6780..7619)
                     /gene="prmC"
                     /locus_tag="VYN69_000973"
     CDS             complement(6780..7619)
                     /gene="prmC"
                     /locus_tag="VYN69_000973"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000761890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEV1533265.1"
                     /translation="MKLAQLFSNFEEELIRQGEEAESLSFVYRSLKNLSFTDFIFALQ
                     QEVTTEEEKQLVEDIYQQLAAHKPAQYIIGQADFYGMHLKVDERVLIPRPETEELVEL
                     ILTENLETNLSVLDIGTGSGAIALALAKNRPDWSVTAADVSQEALELASENASDQKLN
                     IFFKKSDCFAEISEKYDIIVSNPPYISREDESEVDLNVLHSEPHLALFADEDGLAIYC
                     RIAEDAKDYLKDGGKIYLEIGYKQGQSVPELFRKHLPEKRVRTLKDQFGQDRMVVVDD
                     GQD"
     gene            complement(7619..8698)
                     /gene="prfA"
                     /locus_tag="VYN69_000974"
     CDS             complement(7619..8698)
                     /gene="prfA"
                     /locus_tag="VYN69_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEV1533266.1"
                     /translation="MNIYDQLQAVEDRYEELGELLSDPDVVSDTKRFMELSKEEASNR
                     DTVIAYREYKQVLQNIVDAEEMIKESGGDADLEEMAKQELKDAKAEKEEYEEKLKILL
                     LPKDPNDDKNIILEIRGAAGGDEAALFAGDLLTMYQKYAEAQGWRFEVMEASMNGVGG
                     FKEVVAMVSGQSVYSKLKYESGAHRVQRVPVTESQGRVHTSTATVLVMPEVEEVEYDI
                     DPKDLRVDIYHASGAGGQNVNKVATAVRIVHLPTNIKVEMQEERTQQKNREKAMKIIR
                     ARVADHFAQIAQDEQDAERKSTIGTGDRSERIRTYNFPQNRVTDHRIGLTLQKLDTIL
                     SGKLDEVVDALVLYDQTQKLEELNK"
     gene            complement(8695..9285)
                     /locus_tag="VYN69_000975"
     CDS             complement(8695..9285)
                     /locus_tag="VYN69_000975"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000068157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEV1533267.1"
                     /translation="MAQLYYRYGTMNSGKTIEILKVAYNYEEQGKGVVIMTSALDTRD
                     GVGYVSSRIGMKRPALAIEETTDIFGYIRDLPEKPYCVLVDEAQFLKRHHVYDLARVV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLLADKIDEIKTICQYCKKKATMVLRTQDGL
                     PVYDGEQIQIGGNETYISVCRKHYFAPEINKENEEK"
     gene            9408..9590
                     /locus_tag="VYN69_000976"
     CDS             9408..9590
                     /locus_tag="VYN69_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005589823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEV1533268.1"
                     /translation="MPFVRIDLFEGRTLEQKKALAKEVTEAVVRNTGAPQSAVHVIIN
                     DMPEGTYFPQGEMRTK"
     gene            9728..11101
                     /gene="mnmE"
                     /locus_tag="VYN69_000977"
     CDS             9728..11101
                     /gene="mnmE"
                     /locus_tag="VYN69_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002906470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEV1533269.1"
                     /translation="MITREFDTIAAISTPLGEGAIGIVRLSGTNSFAIAQKIFKGKDL
                     SQVASHTLNYGHIVDPLTGKVMDEVMIGAMKSPKTFTREDIIEINTHGGIAVTNEILQ
                     LAIREGARLAEPGEFTKRAFLNGRVDLTQAEAVMDIIRAKTDKAMNIAVKQLDGSLSD
                     LINNTRQEILNTLAQVEVNIDYPEYDDVEEATTAVVREKTMEFEQLLTNLLRTARRGK
                     ILREGISTAIIGRPNVGKSSLLNNLLREDKAIVTDIAGTTRDVIEEYVNINGVPLKLI
                     DTAGIRETDDIVEQIGVERSKKALKEADLVLLVLNASEPLTAQDRQLLEISQDTNRII
                     LLNKTDLPETIETSKLPEDVIRISVLKNQNIDKIEERINNLFFENAGLVEQDATYLSN
                     ARHISLIEKAVESLQAVNQGLELGMPVDLLQVDLTRTWEILGEITGDAAPDELITQLF
                     SQFCLGK"
     gene            complement(11148..12083)
                     /gene="dapA"
                     /locus_tag="VYN69_000978"
     CDS             complement(11148..12083)
                     /gene="dapA"
                     /locus_tag="VYN69_000978"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEV1533270.1"
                     /translation="MSYQDLKECKIITAFITPFHEDGSINFDAIPALIEHLLDHHTDG
                     ILLAGTTAESPTLTHDEELELFAAVQKIVNGRVPLIAGVGTNDTRDSIEFVKEVAEFG
                     GFAAGLAIVPYYNKPSQEGMYQHFKAIADASDLPIIIYNIPGRVVVELTPETMLRLAD
                     HPNIIGVKECTSLANMAYLIEHKPEEFLVYTGEDGDAFHAMNLGADGVISVASHTNGD
                     EMHEMFIAIAESDMKKAAAIQRKFIPKVNALFSYPSPAPVKAVLNYMGFEAGPTRLPL
                     VPAPEEDAKRIIKVVVDGDYEATKATVTGVLRPDY"
     gene            complement(12139..13215)
                     /locus_tag="VYN69_000979"
     CDS             complement(12139..13215)
                     /locus_tag="VYN69_000979"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000542485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEV1533271.1"
                     /translation="MGYTVAVVGATGAVGAQMIKMLEESTLPIDKIRYLASARSAGKS
                     LKFKDQDITIEETTETAFEGVDIALFSAGSSTSAKYAPYAVKAGVVVVDNTSYFRQNP
                     DVPLVVPEVNAHALDAHNGVIACPNCSTIQMMVALEPVRQKWGLDRIIVSTYQAVSGA
                     GMGAILETQRELREVLNDGVKPRDLHAEILPSGGDKKHYPIAFNALPQIDVFTDNDYT
                     YEEMKMTKETKKIMEDDSIAVSATCVRIPVLSAHSESVYIETKEVAPIEEVKAAIAAF
                     PGAVLEDDVAHQIYPQAINAVGSRDTFVGRIRKDLDAEKGIHMWVVSDNLLKGAAWNS
                     VQIAETLHERGLVRPTAELKFELK"
     gene            complement(13557..13629)
                     /locus_tag="VYN69_000980"
     tRNA            complement(13557..13629)
                     /locus_tag="VYN69_000980"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13594..13596),aa:Thr,seq:ggt)
     gene            complement(13729..14238)
                     /locus_tag="VYN69_000981"
     CDS             complement(13729..14238)
                     /locus_tag="VYN69_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEV1533272.1"
                     /translation="MKKLTIRDVADIAIVAAIYVVLTVTPPLNAISYGAYQFRISEMM
                     NFMAFYNPKYIIGVTIGCMIANFFSFGLLDVFVGGGSTLVFLSLGVWLFAKYSKDYLF
                     NGLIRKDHFFFSILFSISMFTIAAELNIVAELPFFLTWFTTGIGEFASLIIGAIIIGK
                     IGRRIDLSK"
     regulatory      complement(14236..14332)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01054"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class II; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF01054"
     gene            14361..14792
                     /locus_tag="VYN69_000982"
     CDS             14361..14792
                     /locus_tag="VYN69_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEV1533273.1"
                     /translation="MKIPIQKFFNNEILAYLFFGLATTLVSILSRLVIYQLSHQELLA
                     TALANIIGILFAFITNDTIVFKQKKKNRLIRLVKFSLARLSTFLLDLFLTFLFVTQFP
                     HIIGQFVTENIDKINAIETIIAQIFIIIINYILSKAYIFRK"
     gene            14874..15251
                     /gene="mscL"
                     /locus_tag="VYN69_000983"
     CDS             14874..15251
                     /gene="mscL"
                     /locus_tag="VYN69_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEV1533274.1"
                     /translation="MLKNLKSFLLRGNVIDLAVGVVIASAFGAIVTSLVNDIITPLIL
                     NPALKAAKVERIAQLSWHGVGYGNFLSAIINFIFVGTALFFIIKGIEKAQKLTGIKKE
                     KTAEKKPTELEVLQEIKALLEKK"
     gene            complement(15293..16387)
                     /gene="hemH"
                     /locus_tag="VYN69_000984"
     CDS             complement(15293..16387)
                     /gene="hemH"
                     /locus_tag="VYN69_000984"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HEV1533275.1"
                     /translation="MKKAILMMTFGSPEEITFEGVADFFTNIRRGVRPQDHEIQTLYD
                     NYVRIGGTPLQKITRQEVALVEARLGNEYSVYFANKFSSPFIPDVIGQMEADGIEQCI
                     CLILEPHYSFYSVMGYEKFLESKQIQFLVIKDWYQEEALLNYWADEIAKILKEEVKQD
                     SFKVIFSAHSVPIFALDFGDPYIDQIFENSKLVAEKLGLSSEQYTNTWQSESDIGIPW
                     IKPDVLEYLREQTEHPDHYIFVPISFISEHIEVLFDNDVECYDLCQEFGVNYHRSPMP
                     NTDSRLIDALVNTVRVNENQEFKEFLPEEETFDELVPSDETKNILAESEDLQMPEFVK
                     KLIEKKGRENVKMPYLIKKMLEKAGKLPKE"
     gene            16542..17765
                     /gene="pepT"
                     /locus_tag="VYN69_000985"
     CDS             16542..17765
                     /gene="pepT"
                     /locus_tag="VYN69_000985"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000222045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEV1533276.1"
                     /translation="MTYPNLLDRFLTYVKVNTRSDEHSTTTPSTQSQVDFATNVLIPE
                     MKRVGLQNVYYLPNGFAIGTLPANDPSLTRKIGFISHMDTADFNAEGVNPQVIENYDG
                     GVIELGNSGFKLDPADFKSLEKYPGQTLITTDGTSLLGADDKSGIAEIMTAIEYLTAH
                     PEIKHCEIRVGFGPDEEIGVGANKFDAEDFDVDFAYTVDGGPLGELQYETFSAAGAEL
                     HFQGRNVHPGTAKGQMVNALQLAIDFHNQLPENDRPELTEGYQGFYHLMDVTGSVEEA
                     RASYIIRDFEKDAFEARKASMQSIADKMNEELGSDRVTLNLTDQYYNMKEVIEKDMTP
                     ITIAKAVMEDLGITPIIEPIRGGTDGSKISFMGIPTPNIFAGGENMHGRFEYVSLQTM
                     ERAVDTIIGIVAYKG"
     gene            complement(17801..18430)
                     /locus_tag="VYN69_000986"
     CDS             complement(17801..18430)
                     /locus_tag="VYN69_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533277.1"
                     /translation="MNNANQLLEQIHEEGIRQSLAEEVENLKAATNKADADLDEVNSQ
                     VKDVLTRIASALQQEKENAEQDPQTLVLYQKLYDILMSLHSYLESNNGSDADFDKVDA
                     LLDQLSAKSKDKTALLESTKAILVLNHQIQSKASASEETSPARNAEANGDNTSAENQP
                     NATAESNIETASDENKPSNTRDSKPAESTSENETTESSTTTGNQEKPVE"
     gene            18395..18616
                     /locus_tag="VYN69_000987"
     CDS             18395..18616
                     /locus_tag="VYN69_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533278.1"
                     /translation="MDLFQELICIVQKTFYFFLAICRRLLVAIYHVLLKQESYNPRLQ
                     GLTEIRNPDKTMFVQDAIRFAQQHGFNML"
     gene            complement(18725..19294)
                     /locus_tag="VYN69_000988"
     CDS             complement(18725..19294)
                     /locus_tag="VYN69_000988"
                     /inference="COORDINATES: protein motif:HMM:TIGR01363.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1533279.1"
                     /translation="MLEITLKSPYQFAHILFQPTIVPHGGHYHFIPESDLSAGELAAT
                     YVFNPNDIVRDTGDAYIVRHGDHYHYIPKSSLNNPPSHSNTEEVGSSSSSVLSNTSLH
                     VHHEEEDGHGFDANRIISEDSEGFVIPHGDHNHYIKVQTKGYEAALKNKIPSLQSNYQ
                     PGTFDEKAVLAKVDQLLADSRSIYKDRLS"
     gene            complement(19338..19884)
                     /locus_tag="VYN69_000989"
                     /pseudo
     CDS             complement(19338..19884)
                     /locus_tag="VYN69_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
BASE COUNT         5710 a         4191 c         3791 g         6192 t
ORIGIN      
        1 taattgatac cttgcaagaa aggaataata gctgttccta taccaagtag gaatgtaata
       61 aatgcagaaa gtcatatact gtcattcttt tctattaaat cattttgaag tgtctcaaag
      121 catccatgat ggcttcttct ttttcaggtg tacactgtgg aataatttgt ttatcctttt
      181 tagacttgtt gtaatgttct cgtaattcta ttccacattt cttttttatc tgtgcaatat
      241 ataatgtcga aacttttaat tcaaatttat tccaaacata ttctttgatt tgagcatatg
      301 ttgctttgct ctccgcactt gtcaaattca tctcatccag ctcaatttca acacttatgt
      361 gcttatcgac atcgagtttg gacaaaagtg ctaccgtctc gacgtgatgc gtttgaggaa
      421 atagatccac cggctggact ttcttcaatt catatcccaa ttcttggtag agtttgatat
      481 cacgcgccat ggttgcgaca ttgcaggaga tataggcgat gcgatcggct cctgtttggg
      541 cgcttgcttt gataaagctt tctgtcaagc ccttgcgtgg aggatcaacc aagataacgg
      601 ttggttgaat cccatcttta agccaattct tcatagcatt ttcagctgtg tcacagacat
      661 agtgggcgtt tgaaatattg ttcaactgag catttttttt actattctca accgcttctg
      721 gaatcagttc aacaccgtag acttctttga catgcttggc gactgataaa ccaatggttc
      781 caataccaga ataagcatca ataaccacgt catctttttt taactctgca aagtcaatgg
      841 ctgtttgata gagtttctcc gccatttcag tattgacttg gtaaaaggct gggccagcga
      901 tttggaagtc atttcccaac atctggtccg taatatagtc ttgaccataa agagtgcgcc
      961 actccttacc aaaaatcgca ttggtattct ggtcgttgat attttgcatg acagacacaa
     1021 tctctgggaa ctgcttgata acttgttcaa tcaattggtc aacacgaaaa acttttggac
     1081 gagttgccac caaaacgacc atgatttgtc ctgaatagtg accacgacgc accacaagat
     1141 tccgaatcaa tccagactgt tccttttcgt cataaggttt taaatcaaaa cgacggagca
     1201 ggtctcgaag agctactacg acttggtcaa tgacaggatc ctggataaag aaatcttcaa
     1261 ggggcatgag gttgtgcgaa ttcttacgga aaaatcctgt ttccaagaca ccattcactc
     1321 gacgaacggg cacctgcgcc ttattgcgat acttgactgg atgttccata ccaagcgttt
     1381 cagcaacttc tacatctgca attccagcaa tcttgtagag actgttcttg acttgcttgg
     1441 ttttaaactt gagctgttct ggataagaaa ggtgtcctaa atccgcgatt cctgaacgca
     1501 ggtaagccaa atctagatct tgattacggt gtggtgactg gacaaggtat ttttcaactt
     1561 ttccaaagcc aatcttttta ttgaccttga ggacacgcat gagaattttt tcactcggta
     1621 aagcattctc tacaaaaaag accaaaccat ctaccttggc aactcctgcc ccttcatggg
     1681 tcaaatcaac aatttcaact tctacaatat catttttctt taacattctt ttctctttct
     1741 attggccgac aaaaaagacg gcaacgttac ggttaccatc cattatacca tgaaatttct
     1801 taagaaccaa aactcttgaa gaagttgaca agggcttgcc agagggagct taagaagtta
     1861 ccaccgtcct ctagcgcctt atccgcatca aagttaaggt tgatattttt aaaactgtcg
     1921 ccagcttgtg atacgatgct ttgtttaagg tcatttaggg ttttagtgaa atctgcattg
     1981 ctgaggatat cactctttga gagattcaag gcaaaattga tgatgatatt gatctggttt
     2041 cctgttatga cctgatcaag tttgtaattt tttaaggtat cttcaacaat cttgcggata
     2101 tcttcttctg tcagatttcc cttgctttct ttagctttgg cgagtcctga cttgatatca
     2161 gctagggcaa cgtttaattt attagcatca tagcctgatt tgtccttgtt ttcagcattg
     2221 atatctgaca aagcttttag ctcttcttga gccaaatctt tattggcttg cggcaccttg
     2281 gctccattag cttctagcga atagtagatc ccagccaaag cactctcacc tgtaactgga
     2341 atgggtgctg ctacagtgat tttggcgtgt tccacaccca aagttacggc tgcatttcgg
     2401 tacatatcct gagttacctt agtgatgttt tcaggtgttt caatcttgac ctcaagtggc
     2461 gatttgtcac ctagcttttg aatcttggct gatgaataca actgtaagct agagtcattg
     2521 gccacattca tgattttaga ataaacatca ggtgtcatgg tcttgagttc tttggtatct
     2581 gttgaggcat tgtagcccag ttttttaaga gtttgatttt tttggtcttc agatagggag
     2641 gaacctagga catattcagg ttggacatag gtttcatcga taactttttg aacatctgtt
     2701 gctgcatgga cgctattcat agctgttact gcccacaaga ccgcagcact agtcagaaag
     2761 agtttctttc tcatagggaa tttcctcctt tacttcttta gagtaatata tctatcttaa
     2821 agaaaactta taacaaaaac acctggtcta gccagatgtt gaaaagagag tgaaacattt
     2881 gatgatgtaa aggttaagtc gtacctgtct agaataataa tagtttcctc catttacata
     2941 gagttcagca ccgtgaaaaa tggaaatggg atgaatataa ctataagtct ttccagtcgt
     3001 attaccaagc aagggggcaa cagtctcacg agagtactgt ttggctagag ccaaggtatt
     3061 ttccttgcca ttttgggcga taaaatcgat ataggcaggt ccaaaattat aggcttgaac
     3121 agctgtccag atatctacac ccttcttctg cgccagatag agattgcctg tcagagtttg
     3181 aatgccttgc cgaatgctag aggcattatc attgatggtg ttggtggaac cacttgcaga
     3241 ctcactagac tgcataacat cgccttcttt tccttttgtt tcagtataaa tcatagcaag
     3301 cacaagctct tcgtttgctg gggtgtcttg ttcactcaag atttctcgca ccatgggttg
     3361 ataggtcatg acttgtttga catcttgatg aacgcggtaa gctttatagc cagcaaaaag
     3421 gaagactgct agtacaagca ctcttcgaat tcgtttaaac attatttact ttggatatcc
     3481 tcgatatttt tgattaagat agagtaggtt ccattttcgt tttggataaa ctcaacagac
     3541 tcggcgtctt gatagacgtt attgggaacg atgagctcaa ttccatttga taaggagagt
     3601 ttttggtttt caaatttctt taattggcga ctggcatcaa tttcatcaaa ttgaacaggt
     3661 tctggtacgg cttctttgac ttggtcaata aagctcaaac gagccgtcag attgttgtca
     3721 aaaaggtcat tagccaattt ctcaggtgac aattcattgc tttcttctag gttgttgaaa
     3781 atcgctgatt tgaccttgga ttgaaattga aaatcatctg tgttaaaaga ttcagcaatt
     3841 ctctgggctg ttttttccag ttccttgata gattttttag gagaaatctt aggagcgaca
     3901 gcaagaagat tatctgaaaa atagttcaaa aaagtcccgt tgtacttgat tcgtttttca
     3961 atcaggtgat acttgcgact ctgaagattg accaccaagg cctcgtcagc acccgttcca
     4021 aatccaggca ggttattctg agtcagcttg attggattat caacttctcc tccgaggtgg
     4081 gtcaaggtct cccgcagggc aattcgcaag aaagcgaaat gttctacacc ttctttagaa
     4141 aattgaacaa aaatcaagtc attggtcttg agattttcag aaatgctaaa ctcctctttc
     4201 cagagattag ccagcgttac tgatgtctcc aacaaatcgt ctgtaatatg attgaagaag
     4261 ggattttctt cttcgaaaat cccagtcttg gcttcatctg aatacacatg ttcaattttt
     4321 ttacgtaggt attcttcgat ttttggagta atattgagaa acttatctgc taagaacagc
     4381 tcggtatcat ccggactgaa ctggtgaata atggctttct taatataaat gtccataaaa
     4441 gttttattcc tcgtataatg ggaaggcatc tgtcaattct ttgactgcac ttctcacttc
     4501 ttctaataca gcctcatttt ctgaattctt aagggtttta atgatgagtt cagccacttt
     4561 gcgactttct tcttcaccaa attcacgtgc agtaatagct gctgctccga tacgaatccc
     4621 acttgtcttg aatggtgaca aggtttcgta agggattgaa tttttattta gggtaatatt
     4681 gacttcatcc agcaagtttt gagcaacttt tccgttttct acaaccttag tcacatcaac
     4741 aaggaagaga tggttttcag ttccacctga aataatacgg aaatcagggt cttgcaagaa
     4801 gacatctgcc atagccttgc tgttcttaat tacattggcg gcatattcct tgaaggctgg
     4861 atccaaaact tctttgaagg aaactgcctt agccgccaca acatgctcta aaggaccgcc
     4921 ctgaatacct gggaaaatag ctgaattgat ttttttagca agttcttcgt cattggtcaa
     4981 aatcaaacca ccacgaggtc cacgaagggt tttgtgggtc gttgttgttg tgatatgagc
     5041 gtatggaact gggcttggat gaaggccagc cgcaaccaaa ccagcgatat gggccatgtc
     5101 caccatgagc ttcgcaccga cagcatctgc gatttcacgg aattttgaaa aatcgataat
     5161 ttgagaatat gctgaagcac cagctacaat cagttttggt tttacttctt gggcttgttt
     5221 caagatagca tcaaagtcta agagttccgt ttcaggatca acactataag aaacaaagtt
     5281 gtaggtttga ccagagaagc taacaggagc cccatgagtc aaatgaccac ctgctgccaa
     5341 atccattccc ataaccgtat cacctggctc aatcaaggcc atgtaagccg cacagttggc
     5401 ctgacttcct gagtgaggtt ggacattggc aaatttagca ccgaaaattt cttttgcgcg
     5461 ttcaatggct agagattcta ccacatctac tacatcagtt ccaccataat aacggcgtcc
     5521 tgggtaaccc tcggcatatt tgtttgtcaa gatagaccct tgagctgcca taacagcctt
     5581 ggaaactacg ttttccgaag caatcaactc gatattgttt tgctggcgtt cttcttcttt
     5641 ggcaatagca ttccagagat cagcatcgta tgctttaaaa tcatctttat caaaaatcat
     5701 aggtcttctc ctttattttg tgactagtcc attagtttga ttttataata agaaaataaa
     5761 ctaaaagatg cgaataaacc gtttctgcat tttatcacaa gtataaccaa ctttttcata
     5821 aaatgcatga gcacccagac gatgattggc agaatttaag cggataaacc cataaccaca
     5881 tcttttggct tcttgttcca acccttgtag taaactttta ccgatacctt gaccttgcgc
     5941 ttgaggtgaa actgctaaag ctaaaatatt aaatcctgct ttggaataga gtgattcgta
     6001 aacttcagcg tggacatatc caagtaagac atgattagct gcatcctcat agccaagtag
     6061 gaaatgatgg gaatcctgag acagtctagc tagttggcta gctgtgtcct ctggactaaa
     6121 agaataaccc aaagcctctt ggttgatgtc acatatcgct ttcacatctg tttcttgcaa
     6181 atctcttagc attttattcc tcctcaaaag aaatctctgg caaccgagca agaatatctt
     6241 ctcgtttaat tgcgccttgg cgtaagattt tcaccttgtc tccagacaaa tccacaatag
     6301 ttgaatcctg tccagttaga aaagcatcgt cttccagacc cagaacctct tggtcaaaat
     6361 ccttcagaat ttgttcaaag gttacaccac ttgcctgacc tgagatattg gcagacggcc
     6421 caatcaaggg acctgtctct cgaattaaat ccagtgtgat agggtgactg ggcatccgaa
     6481 atccaatagt tgcaaggtca aaatttaccc aatagggaac tcggtcattg gcttcgagaa
     6541 taatggtcaa gggacctggc aaaaaggtct ctacaagttt ttgtagataa gctggctgat
     6601 tcttagaaaa gtgcaagata tcctctaaag aggcaacatt gagattgagc gccttgtctc
     6661 taggacgacg tttgagctgg taaacatggt caactgcttt ttcgtctaag gccttggcaa
     6721 aaagaccata aaccgtctct gtaggcagaa tgacagctcc accattttcc aactcttgtc
     6781 taatcctgtc catcatcaac cacgaccatc ctatcttgac caaattggtc cttgagtgtt
     6841 cgaactcttt tttcaggaag atgtttccta aaaagttcgg gaacactttg accttgcttg
     6901 tatccaattt caaggtaaat cttaccacca tccttgagat agtcttttgc atcttccgca
     6961 attctacagt aaatagctag gccatcctcg tctgcaaaga gagctagatg aggctccgaa
     7021 tgcaaaacat tcaagtctac ctctgactca tcttcacgag agatataggg tggattggat
     7081 acaattatat catatttttc agaaatttct gcaaaacaat cagatttttt aaaaaatata
     7141 ttaagctttt gatcgctagc attctctgat gcaagctcta aggcctcttg agaaacatct
     7201 gctgctgtca ctgaccaatc tggtctattt tttgctagag cgagagcaat agctccacta
     7261 cctgttccaa tatccagaac tgagagattc gtctcaagat tttcagttag aataagctcc
     7321 actaactcct ctgtttctgg acgaggaatc aaaacccgtt catccacctt taaatgcatt
     7381 ccataaaaat ctgcctgtcc aatgatgtac tgagctggct tgtgagctgc tagttgctgg
     7441 taaatatctt ctacaagttg tttttcttcc tctgttgtca cctcctgctg gagggcaaaa
     7501 ataaagtctg taaaagatag atttttcaga ctacgataga caaaagagag gctttccgct
     7561 tcctctcctt gtcttatcaa ctcttcttca aaatttgaaa ataattgagc taatttcatt
     7621 atttgtttaa ttcttctagt ttttgtgttt ggtcataaag caccaaggca tccacaactt
     7681 cgtccaattt accagacaaa atcgtatcta gtttttggag ggtcaagccg atacggtggt
     7741 ctgtgacacg gttttgaggg aagttataag ttcggatccg ttctgaacgg tcaccagtac
     7801 cgattgtcga cttacgctca gcgtcttgtt catcctgagc aatctgagca aagtggtcag
     7861 cgacgcgtgc acggataatc ttcatagcct tctcacggtt cttctgctgg gtacgttctt
     7921 cctgcatctc aaccttgata ttggttggca agtgaacgat acgaacggca gtcgcaacct
     7981 tattgacgtt ctgtccacca gcacctgatg cgtggtaaat atcaacacga agatcttttg
     8041 gatcgatatc gtattctact tcttcgactt caggcatgac aagaactgtg gcagttgagg
     8101 tatgaacacg accttggctt tctgtcacgg ggacacgctg cacacgatgg gcacctgatt
     8161 cgtacttaag cttagagtat acagactgac ctgaaaccat agcaaccact tctttaaacc
     8221 caccgacacc attcatagag gcttccatga cttcaaagcg ccagccttgg gcttccgcat
     8281 acttttggta catagttagc aaatctccag cgaaaagtgc cgcttcgtct ccaccagctg
     8341 ctccacggat ttcaaggatg atattcttgt catcgtttgg atcctttgga aggagcaaaa
     8401 ttttcagttt ttcttcatat tcttcttttt cagccttggc atctttgagt tcttgcttgg
     8461 ccatttcttc caagtccgca tctccgcctg attccttaat catctcttcg gcatcgacga
     8521 tattttgaag gacttgttta tactcacggt aggctattac ggtgtcacga ttggaagctt
     8581 cttcttttga aagctccata aaacgcttgg tgtctgaaac gacatcaggg tcactcagca
     8641 attctcctag ttcttcataa cggtcttcta cagcttgtag ttgatcatag atgttcattt
     8701 ttcttcattc tccttattga tttcaggcgc aaaataatgt ttacggcaaa ccgagatata
     8761 ggtttcatta ccaccaatct ggatctgttc tccatcgtaa acgggcagtc catcctgtgt
     8821 tcgcaacacc atggtcgcct ttttcttgca atactgacag atggtcttga tttcgtcaat
     8881 cttgtccgct aaaagcaaga gatatttgga accttcgaac aattcattgc gaaagtcatt
     8941 tttcaagcca aaagccatga cgggtatgtc taactcgtcc acaacacgag ctaggtcgta
     9001 aacatggtgg cgtttaagaa actgggcttc atcgaccaaa acacagtaag gtttttctgg
     9061 taggtctcgg atatagccaa agatatccgt tgtttcctca atcgcaaggg cagggcgttt
     9121 catgccaatt cgactcgaca catagccaac accgtcacgc gtatccagag ccgaggtcat
     9181 aatgacaaca ccttttcctt gctcctcgta gttataggcc actttgagaa tctcaatcgt
     9241 tttaccagag ttcatggtcc cataacgata gtacaactgt gccatgtttc ttgcttcacg
     9301 tccatttcta aatttttgct acattctagt atatcataat tttcttaagc tttaaacggc
     9361 aaaatgtggt aaaatggaag aaatcaaaac tagtggagga agctattatg ccatttgtac
     9421 gcatcgattt atttgaagga cgcacgctcg agcaaaagaa agctcttgct aaggaagtaa
     9481 cggaagcagt tgtccgcaac actggagccc cccaatctgc tgtccatgtc atcatcaacg
     9541 acatgccaga aggaacttac ttcccacaag gggaaatgcg taccaaataa gctagcttaa
     9601 gcagaattgc ttaggctttt tcaatctcca agtaacatcc attgaagaaa tagcctaaat
     9661 ttgttacaat ttgaaaggat acttggaaaa atttccaaga aaagagctat ttattaaagg
     9721 aaacattatg attacacgtg aatttgatac catcgctgct atctctactc cactaggtga
     9781 aggggctatt ggtattgtcc gcctgagcgg aacaaacagt tttgctattg cgcaaaagat
     9841 tttcaaagga aaagacttga gtcaggttgc tagccataca cttaactacg gtcacatcgt
     9901 tgaccctctg actggtaaag tcatggacga ggtcatgatt ggggctatga aatctccaaa
     9961 aaccttcact cgtgaagata ttatcgagat taacacccac ggtgggattg cggtgactaa
    10021 tgaaattctc cagctagcta tccgtgaagg agctcgattg gctgaacctg gtgaatttac
    10081 taaacgtgcc ttcctaaatg gacgtgttga cttgactcag gctgaggcag taatggatat
    10141 catccgtgct aagacagata aagccatgaa cattgcggtc aaacaactag atggttctct
    10201 ttctgacctc attaacaata cccgtcaaga aatcctcaat acacttgccc aagtcgaagt
    10261 taatatcgac tatcctgagt atgatgatgt tgaggaagcg actactgctg ttgtccgcga
    10321 gaaaactatg gaatttgaac aattgctaac caatctcctt aggacagccc gtcgcggtaa
    10381 aatccttcgt gaaggaattt ccactgccat catcggacgt cccaacgttg ggaaatctag
    10441 cctcctcaac aatctcttgc gtgaggacaa ggctatcgtt acagatattg ctggtaccac
    10501 acgtgatgtc atcgaagagt acgtcaacat caatggtgta cctctcaaat tgattgatac
    10561 agccggtatt cgtgaaacag atgacatagt ggaacaaatc ggtgttgagc gttcgaaaaa
    10621 agccctcaag gaagccgact tggttctact agtgctaaat gccagtgaac cactgactgc
    10681 gcaagacaga caacttcttg aaattagcca agataccaat cgcattattc tacttaataa
    10741 aaccgacctg ccagaaacga ttgaaacttc gaaactacct gaagacgtta tccgtatttc
    10801 agtccttaaa aaccaaaaca tcgacaagat tgaagagcga atcaacaacc tcttctttga
    10861 aaatgctggc ttggtcgagc aagatgctac ttacttgtca aacgcccgtc acatttccct
    10921 gattgaaaaa gcagttgaaa gcctacaagc cgttaatcaa ggtcttgagc tggggatgcc
    10981 agttgatttg cttcaagttg acttgactcg tacttgggaa atcctcggag aaatcactgg
    11041 ggatgctgct ccagatgaac ttattaccca actctttagc caattctgtt taggaaaata
    11101 agaaaaatcc atgatccttc attcggtcat ggattttatt gtctttatta gtaatctggt
    11161 cttaagacac ctgttacagt tgccttggtc gcttcgtagt cgccatctac gacaaccttg
    11221 ataatgcgtt tagcatcttc ttctggtgct ggaacaagag gtagacgagt tggtccagct
    11281 tcaaatccca tgtagttcaa aactgcctta actggagcag gacttggata agagaagaga
    11341 gcattaacct taggaatgaa tttacgttga atagctgcgg ctttcttcat atcactttct
    11401 gcaatggcaa taaacatctc gtgcatttca tccccatttg tatgagaggc aacagaaata
    11461 accccatccg ccccaaggtt catggcatgg aaagcatctc catcctcacc agtatagacc
    11521 aagaactctt ctggtttgtg ctcaatcaag taagccatat tagccaagct agtacattct
    11581 ttgacaccga taatatttgg atgatcagcc aagcgaagca tggtttctgg agttaattca
    11641 acaaccacac gccctggaat gttatagata ataattggta ggtcagaagc atctgcaatg
    11701 gctttaaaat gctgatacat tccttcttga gaaggtttgt tgtagtaagg aacaatagca
    11761 agaccagcag caaaaccgcc gaattccgct acttctttga caaactcgat agagtcacgc
    11821 gtatcattag tacctacacc cgcaatcaaa ggaactcgtc cattgacaat cttttgtaca
    11881 gccgcaaata actccaactc ctcatcgtgg gtcaaagttg gactctcagc agtggtccct
    11941 gcgagaagaa ttccatccgt atgatggtcc aataaatgct caatcaaggc tggaatagca
    12001 tcaaagttga tggaaccatc ctcgtggaag ggagtaataa aggctgtgat gattttacac
    12061 tcttttaaat cttgataaga catgaaaaca cctctctatt tcaaagaagg agttcatctg
    12121 aactcctaaa cgatatgact attttaattc aaatttcaat tcagctgttg gacgaaccaa
    12181 tccacgttca tgaagagttt cagcaatctg aactgagttc caagcagcac ctttgagaag
    12241 gttatctgaa acaacccaca tgtgaattcc tttttctgca tccaagtctt tacggatacg
    12301 accaacaaag gtatcacgtg aaccaactgc attgatagct tgaggataga tttgatgagc
    12361 tacatcatct tcaagaacag cacctgggaa ggctgcgata gctgctttta cttcttcgat
    12421 tggagccact tcttttgttt cgatataaac agactcagag tgagctgaca agactggaat
    12481 acgcacacat gttgcagata ctgcaatgct atcatcttcc ataattttct tagtttcctt
    12541 ggtcatcttc atctcttcgt acgtgtaatc attatcagtg aaaacatcaa tttgtggaag
    12601 agcattgaag gcgataggat aatgtttctt gtcaccacct gaaggcaaga tttccgcatg
    12661 caaatcacgt ggtttcacac catcattcaa gacttcacga agttcacgtt gtgtctcaag
    12721 aattgctccc ataccagcac ctgaaacggc ttgataagtt gaaacaatga tacggtccaa
    12781 gccccatttt tggcgaaccg gctcaagagc caccatcatt tggattgttg aacagttagg
    12841 gcaggcaatg actccgttgt gggcatcaag tgcatgagca ttgacctctg gaacaaccaa
    12901 aggaacatct ggattttgac ggaaataaga tgtattatct actactacca cgccagcttt
    12961 tactgcgtat ggtgcatact tagctgatgt agaactacct gctgaaaaga gagcaatatc
    13021 aactccttca aaagctgttt cagtcgtttc ttcaattgta atatcttgat ctttaaattt
    13081 caatgactta cctgctgaac gtgcagaagc aaggtaacgg attttatcga ttggaagtgt
    13141 tgattcttcc aacattttta tcatctgagc accgacagca cctgtcgcgc cgactacagc
    13201 aactgtatat cccataaata acctctttcg gaattttcta aaaatttcta taatagaagt
    13261 attatactac tttttccatg aattgcaaag ctttttcatc attttttgga aaataaaaat
    13321 cccagtcgct agactaggga ctaagaggca tcttcatgtg atgagcaggt tcacacaact
    13381 catcaaggtc cgctcctgcg ttatgacctc cttatgctca atagtagtcc gaagactacc
    13441 tatcgactca tcgcatattc tatattacta gagatacttg attttgtcaa taagaaattt
    13501 tttcgatcga gtataacaaa aaactctcgt attcaagagt ttccctatac atatcatgct
    13561 aattgccggg atcgaaccgg cgacctcatc cttaccatgg atgcgctctg ccaactgagc
    13621 taaatcagct tacttaagaa gtatactata ttcagaaaaa cttgtcaagt ttccttagaa
    13681 attttccata ggaaaaagcc aggtaagaac tacctagctt atcttcttct atttacttaa
    13741 atcaattcga cgaccaattt taccaataat aatcgcccca ataatcaatg aagcaaattc
    13801 accgattcct gttgtaaacc aagtaaggaa gaatggtaat tctgctacaa tatttaactc
    13861 tgcagcgata gtaaacatgg aaattgaaaa gaggattgaa aagaagaaat gatcttttcg
    13921 aatcaatccg ttaaagaggt aatccttgct atattttgca aaaagccaaa cacctaaact
    13981 gaggaatact aaggttgaac caccaccaac aaagacatct agcagtccga agctaaagaa
    14041 attagcaatc atacaaccga tggtaactcc gatgatgtac ttaggattgt aaaaagccat
    14101 aaagttcatc atttctgaaa tacgaaactg ataagcacca tagctgatgg catttagggg
    14161 cggtgtgaca gtcaaaacga cgtagatagc agcgacgatt gcaatatctg caacatcacg
    14221 aatagttaat tttttcatct tttctccttt ggcggttgcc cgcgtgtaat atgcttggtg
    14281 aaagaagcta agcaccaagg gttgctttat tcaaccctac tagtatagca caatagcctg
    14341 ctttatgcta tacttaacac atgaaaattc ctattcaaaa attttttaac aatgaaatct
    14401 tagcctatct cttttttggc cttgcgacaa ctctagtttc gattctctca cgactagtca
    14461 tttatcaact aagtcatcag gaactcctgg ctactgccct agcaaatatt atcggtatcc
    14521 tatttgcctt tatcacaaat gatacgattg tatttaagca aaaaaagaaa aatagactta
    14581 tccgtttagt gaaattttct cttgctcgcc tttctacttt tctgttagac ttgtttttaa
    14641 cttttctctt tgtaacccaa tttcctcaca ttatagggca attcgtaact gaaaacattg
    14701 ataagataaa tgctattgaa acaataattg cacaaatatt cattattatc atcaattata
    14761 tcttgagcaa ggcctatatt tttagaaaat aatgctccat ttatgagcct tttaattgcc
    14821 ttctataatg atttaggata caatgaaaca cgaaacattg aaaggaaaac agtatgttaa
    14881 aaaatctaaa atcgttcttg cttcgaggaa atgttattga ccttgctgtc ggtgttgtca
    14941 ttgcctctgc ttttggtgct atcgttactt cacttgtaaa cgacattatc actcctctta
    15001 ttttaaatcc cgctttgaaa gctgctaaag ttgaacgtat cgctcaactt tcttggcatg
    15061 gagtcggcta tggtaacttc ttaagtgcta ttatcaattt tatctttgtg ggtaccgccc
    15121 tcttctttat tatcaagggc attgaaaaag cacagaaact gactggcata aagaaagaaa
    15181 aaactgccga aaaaaaacca accgaattgg aagtccttca agaaataaaa gctctccttg
    15241 agaaaaaata atcgattaaa aggatctagc gcaaagctaa atcctttttt ctttactctt
    15301 tgggtaactt gcctgctttt tcaagcattt ttttgatcag ataaggcatc ttaacattct
    15361 cccgcccttt tttctcaatc agttttttca caaattctgg catttgtaaa tcttcagatt
    15421 cagctaaaat gtttttcgtc tcgtctgaag gaactaactc atcaaaggtc tcttcttctg
    15481 ggagaaattc cttaaactct tgattttcat tgactctaac tgtattgacc aaagcatcaa
    15541 tcaaacgaga atctgtattt ggcattggtg aacgatggta gtttacccca aattcctggc
    15601 ataagtcata acattctaca tcattgtcaa acaagacctc aatatgctca ctgatgaaac
    15661 taataggtac aaaaatataa tggtctggat gttctgtctg ttctctgaga tactccaaaa
    15721 catctggttt aatccatgga ataccaatat cactctcact ctgccaagtg ttggtatatt
    15781 gttctgaact caagcccagt ttttcagcga ctagcttgct attttcgaaa atttggtcta
    15841 tataaggatc accaaagtcc aaggcaaaaa tgggcacact atgggctgaa aagatgactt
    15901 taaagctgtc ttgctttact tcttctttta aaatcttagc aatttcatct gcccaatagt
    15961 ttaagagcgc ttcttcctga taccagtcct taatgaccaa aaattgaatt tgtttgcttt
    16021 ctaaaaattt ttcatagccc atgacagagt agaaagaata atggggctcc aaaatcaagc
    16081 aaatacactg ttcaattccg tctgcttcca tttgaccgat cacatctgga ataaagggac
    16141 tggaaaactt attagcaaag tagacactgt attcattccc cagcctagct tctaccaagg
    16201 caacctcttg acgggtaatt ttttgcagag gcgtgcctcc aattcgtaca taattatcat
    16261 agagagtctg aatctcgtgg tcttggggtc tcaccccacg acgaatattt gtgaaaaaat
    16321 cagctacacc ttcaaaggta atctcttctg gtgaaccgaa ggtcatcatt aaaattgctt
    16381 ttttcatgac tgcttccttg tgatgttttt atcaagttta ttttatcatt aattcatcga
    16441 taagtaaacg ctaacatagg gacattctca atcttctgtc ttttgttttt ctcttctttc
    16501 tatgatacaa tggaaaaaat aaattcaaaa ggagtttttt tatgacttat ccaaatctct
    16561 tggaccgctt cttaacctac gttaaggtca acacgcgctc tgatgaacac tctactacta
    16621 ctccaagtac acagagtcag gttgacttcg caacaaatgt cctaattcct gaaatgaaac
    16681 gtgttggact gcaaaatgtt tactatctac cgaatggttt tgctattgga actttgccag
    16741 ccaacgatcc gtctttaaca cgtaagattg gttttatatc gcacatggat actgctgatt
    16801 ttaatgctga aggagtcaat ccacaggtaa ttgaaaacta cgatggtggt gtgattgaac
    16861 tagggaattc tggtttcaaa ctcgatccag ctgacttcaa gagtcttgaa aaatatccag
    16921 gacaaacgct catcacaaca gatggaacaa gcttgctagg tgctgatgac aagtcaggaa
    16981 ttgctgaaat tatgacagcc attgaatatc taactgctca tcctgaaatt aagcactgtg
    17041 agattcgtgt tggttttggt ccagatgaag aaatcggtgt tggtgccaat aaatttgatg
    17101 cagaagattt tgatgtggat tttgcctaca ctgttgatgg tggtccacta ggtgaacttc
    17161 agtacgagac tttctcagcc gctggtgctg aattgcattt ccaaggccgt aatgtccacc
    17221 ctggtactgc caaagggcag atggtcaatg cccttcagct agcaattgat tttcataatc
    17281 aacttccaga aaatgaccga cctgagttaa ctgaaggtta ccaaggtttt taccatctaa
    17341 tggatgtgac aggtagtgtt gaggaggcgc gtgcaagcta catcattcgt gattttgaaa
    17401 aagatgcctt tgaagcgcgt aaagcatcca tgcaatctat cgctgataag atgaatgaag
    17461 aacttgggag cgaccgtgtc actctcaact tgacagacca gtactacaat atgaaagaag
    17521 tcattgaaaa agatatgact ccaattacca ttgctaaagc cgttatggaa gatctaggta
    17581 tcacgcctat tatcgaacca atccggggtg gaacagacgg ctctaagatt tcctttatgg
    17641 gaatcccaac tccgaatatc tttgcaggtg gcgaaaatat gcacggacgt tttgaatacg
    17701 ttagccttca gactatggaa cgtgcagttg ataccatcat tggcatcgta gcttataaag
    17761 gctaaaaaga cgaggtagct cagctacttc gcctttcttt ttattctact ggtttttctt
    17821 gatttccagt agttgtagaa gattctgttg tttcattttc tgaagttgat tcagcaggtt
    17881 tagaatctct tgtattgctt ggtttgtttt cgtcgctagc agtttcaatg ttagattctg
    17941 cagttgcgtt tggttggttc tcagcactgg tgttatcacc atttgcttca gcatttcttg
    18001 ctggacttgt ttcttcactt gcgctagctt ttgactggat ttgatgattc aaaactagaa
    18061 tagcttttgt cgattcaagt aaagctgttt tgtctttact cttagcagaa agttgatcta
    18121 ataatgcatc caccttatca aagtccgcat cagatccatt attactttct aaataagagt
    18181 gaagcgacat gagaatatcg tagagttttt gatagagtac aagtgtctga ggatcttgct
    18241 cagcattttc cttttcttgt tgaagggcgc tagcgatacg agtcaagaca tcttttacct
    18301 gactgtttac ttcatccaag tctgcatcag ccttgtttgt ggcagctttt agattttcta
    18361 cttcttctgc caaggattgt ctgattcctt cttcatggat ttgttccaag agttgatttg
    18421 cattgttcaa aagactttct acttcttcct tgctatctgt cgcagattat tggttgctat
    18481 ctaccatgta ctcctaaaac aggagagtta taatccaaga ttacaaggcc ttacagaaat
    18541 aagaaatcca gataagacaa tgttcgtcca agacgctatt cgcttcgcac agcagcacgg
    18601 attcaatatg ctttaatttt aaagtttagg tgtcaagacc tctttttagt gtgcccaaaa
    18661 tttagagaag taatcaatca actaactttt atttttttca aactttcagt aaactgacct
    18721 aaagctaact caatctgtct ttgtagatgc ttctgctatc agctagaagt tgatctactt
    18781 ttgccaagac tgccttctca tcaaaagttc caggttgata gttggattgc agggatggaa
    18841 tcttgttttt caaagccgct tcatatccct tagtttgaac cttgatgtag tgattgtggt
    18901 cgccatgagg aattacaaaa ccttctgaat cttcacttat aattcgattg gcatcaaaac
    18961 catgaccatc ttcttcctca tggtggacat gtagtgacgt attacttaat acagaactag
    19021 aagaacttcc tacctcttcc gtgttagagt gtgatggggg attgttaaga gatgacttag
    19081 gaatatagtg atagtgatcc ccatgtctta ctatataagc atcacctgta tctctgacaa
    19141 tatcattagg gttaaagaca tatgtggctg ctaattcacc tgccgacaag tcactctcag
    19201 gaatgaaatg atagtgacca ccatgtggta ctatagtagg ttgaaataga atatgagcaa
    19261 attgataagg ggattttaaa gtaatttcta acaatgattt agaaactatg atgtgctatt
    19321 ctaaattcaa ctcactatat ataaccatca tcggtagtat aacgtccctg taattttgct
    19381 acagatactt ctgcactagc tcctttatcg tctttaccat gttcttgttt ttggcgattg
    19441 atttcatctt ttgttcgtac attttctgca tgagcttgat ctttaaggta aacataatac
    19501 tttccatcta ccttaataat atatcctccc ttaacctaac tgacgatatc ttgatctttc
    19561 ggctgatagt tgggggcttt cattaatagc tcttcactaa agagcgcatc aaaaggaact
    19621 ttaccattat agtagtgata atgatcgcca tgagaagtta cataaccttg acctgtaatc
    19681 ttaataacaa tttgttttgc ttgaattcct tctttttgac taacctagtc tggagtcaaa
    19741 ttttcagtct tcttagtgtc tttattactg tttacatatg aaacacgatt tttatctgta
    19801 ttggcctgtt agctatgttg gttcagagca taaacacaca gacttaagga aaggataaca
    19861 acagatccag ctgctatata tttc
//
LOCUS       DAVLVP010000029        19530 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.029, whole genome shotgun sequence.
ACCESSION   DAVLVP010000029 DAVLVP010000000
VERSION     DAVLVP010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 19530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19530
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.029"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            384..767
                     /locus_tag="VYN69_000990"
     CDS             384..767
                     /locus_tag="VYN69_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunity protein"
                     /protein_id="HEV1533280.1"
                     /translation="MKYRLFFVIFLSSVLDILLGTFLQISIVSIGWLVLYSGLFEAGV
                     FLLANKGVAVKIKEVDIRNRFKFIFGKTLWFQILLLIFLIIKLYLGLDARLILFYGHI
                     FIVFNALMYLLSSSQVSLKKNKLSS"
     gene            819..1508
                     /locus_tag="VYN69_000991"
     CDS             819..1508
                     /locus_tag="VYN69_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000760553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1533281.1"
                     /translation="MKKYQLLFKISAVFSYLFFVFGLSQMTLIIQNYWQFSSQIGNFV
                     WIQNFLSLLFSGVMIWILVKTGHGYLFHIPRKKWLWYSILTVLVVVLQISFNVQTAKH
                     VQSTAEGWAVLIGYSGTNFAELGIYITLFFLTPLMEELIYRGLLQHAFFKHSRFGLDL
                     LLPSILFALPHFSSLPSLLDIFIFATSGIIFASLTRYTKSIYPSYAVHVINNIFATLP
                     FLLTFLHRVLG"
     gene            1550..1783
                     /locus_tag="VYN69_000992"
     CDS             1550..1783
                     /locus_tag="VYN69_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533282.1"
                     /translation="MYKHLFFLDSKTLDRLTPYILVLASDTIAFNVFVLTFVSAVVFN
                     FLNSMLALMAIFIGAGYVVGFWLLILNENQRAN"
     gene            1934..2545
                     /locus_tag="VYN69_000993"
     CDS             1934..2545
                     /locus_tag="VYN69_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1533283.1"
                     /translation="MEFFDKFHALCFGFLVLIIVITVPYTINHGGFFQNESALILVSL
                     LVTSLSVAYARKFEMISFGMLSKKQLLLFIAIFLLSVLETLVYIHFFAVSSGSGVQHL
                     AEVSRGISLSLILTTSVFGPIQEELIFRGLLQGAVFDNSWLGLVLTSSLFSFMHGPSN
                     VPSFIFYLLGGLLLGFAYKKSQNLWVSTLVHMFYNSWPLLYYL"
     gene            2706..3500
                     /locus_tag="VYN69_000994"
     CDS             2706..3500
                     /locus_tag="VYN69_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000363010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEV1533284.1"
                     /translation="MDLGDNELTLTPIPGKSGKAYMGSYPDGKRIFVKMNTSPILPGL
                     AREQIAPQLLWSRRLADGRDMCAQEWLTGKILTPYDMNRKQIVNILTRLHRSRPLMTQ
                     LSRLGYAMETPVDLLQSWQETAPDALRKNHFISEVMADLRQTIPGFREDHATIVHGDV
                     RHSNWIETDSGLIYLVDWDSVRLTDRMFDVAHMLCHYISEHQWKEWLTYYGYKYNQTV
                     LSKLYWYGQLSYLSQISKYYMNQDLENVNREIHGLRHFRDKYGKRR"
     gene            3497..4132
                     /gene="trmB"
                     /locus_tag="VYN69_000995"
     CDS             3497..4132
                     /gene="trmB"
                     /locus_tag="VYN69_000995"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001266107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEV1533285.1"
                     /translation="MRVRNRKGATELLEANPQYVVLNPLEAKAKWRDLFGNDNPIHVE
                     VGSGKGAFVSGMAKQNPDINYIGIDIQKSVLSYALDKVLEVGVPNIKLLWVDGSDLTD
                     YFEDGEIDRLYLNFSDPWPKKRHEKRRLTYKTFLDTFKRILPENGEIHFKTDNRGLFE
                     YSLVSFSQYGMKLNGVWLDLHASDFEGNVMTEYEQKFSNKGQVIYRVEAEF"
     gene            4258..4737
                     /gene="rimP"
                     /locus_tag="VYN69_000996"
     CDS             4258..4737
                     /gene="rimP"
                     /locus_tag="VYN69_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEV1533286.1"
                     /translation="MDAIATIVELVREVVEPVIEAPFELVDIEYGKIGSDMILSIFVD
                     KPEGITLNDTADLTEIISPVLDTIKPDPFPEQYFLEITSPGLERPLKTKDAVAGAVGK
                     YIHVGLYQAIDKQKVFEGTLLAFEEDELTMEYMDKTRKKTVQIPYSLVSKARLAVKL"
     gene            4781..5917
                     /gene="nusA"
                     /locus_tag="VYN69_000997"
     CDS             4781..5917
                     /gene="nusA"
                     /locus_tag="VYN69_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000032293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEV1533287.1"
                     /translation="MSKEMLEAFRILEEDKGIKKEDIIDAVVESLRSAYRRRYGQSDS
                     VAIDFNEKTGDFTVYTVREVVDEVFDSRLEISLKDALAINSAYELGDKIKFEEAPAEF
                     GRVAAQSAKQTIMEKMRKQTRAITYNTYKEHEQEIMSGTVERFDNRFIYVNLGSIEAQ
                     LSKQDQIPGEVFASHDRIEVYVYKVEDNPRGVNVFVSRSHPEMIKRLMEQEIPEVYDG
                     TVEIMSVAREAGDRTKVAVRSHNPNVDAIGTIVGRGGANIKKITSKFHPARYDAKNDR
                     MVPIEENIDVIEWVADPAEFIYNAIAPAEVDQVIFDENDSKRALVVVPDNKLSLAIGR
                     RGQNVRLAAHLTGYRIDIKSASEFEAMEDAASVELEVENDTVEE"
     gene            5939..6232
                     /locus_tag="VYN69_000998"
     CDS             5939..6232
                     /locus_tag="VYN69_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000857559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEV1533288.1"
                     /translation="MKTRKIPLRKSVVSNEVIDKRDLLRIVKNKEGQVFIDPTGKANG
                     RGAYIKLDNAEALEAKKKKVFNRSFSMEVEESFYDELIAYVDHKVKRRELGLE"
     gene            6225..6524
                     /locus_tag="VYN69_000999"
     CDS             6225..6524
                     /locus_tag="VYN69_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEV1533289.1"
                     /translation="MNKQKISNLLGLAQRAGRIISGEELVVKAIQDGKAKLVFLAHDA
                     GPNLTKKIQDKSHYYQVEIVTVFSTLELSIAVGKSRKVLAVTDAGFTKKMRSLME"
     gene            6541..9333
                     /gene="infB"
                     /locus_tag="VYN69_001000"
     CDS             6541..9333
                     /gene="infB"
                     /locus_tag="VYN69_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEV1533290.1"
                     /translation="MSKKRLYEIAKELGKESKEVVARAKELGLDVKSHSSSVEEAVAA
                     KIAASFKPAAAPKVEAKPAAPKVSAEKKAEKSEPAKPAVAKEEAKPAEPVAPKTEKVA
                     AKPQSRNFKAEREARAKEQAERRKQNKGNNRDQQQNGNRQKNDGRNGGKQGQSNRDNR
                     RFNDQAKKQQGQQKRRNERRQQEDKRSNQAAPRIDFKARAAALKAEQNAEYARSSEER
                     FKQYQAAKEALAQANKRKEPEEIFEEAAKLAEQAQQVQAVVEVVPEKKEPAVDTRRKK
                     QARPDKNRDDYDHEEDGPRKQQKNRSSQNQVRNQKNSNWNNNKKNKKGNNKNNRNQTP
                     KPVTERKFHELPTEFEYTDGMTVAEIAKRIKREPAEIVKKLFMMGVMATQNQSLDGET
                     IELLMVDYGIEAKQKVEVDNADIERFFVEDGYLNEDELVERPPVVTIMGHVDHGKTTL
                     LDTLRNSRVATGEAGGITQHIGAYQIVENGKKITFLDTPGHAAFTSMRARGASVTDIT
                     ILVVAADDGVMPQTIEAINHSKAANVPIIVAINKIDKPGANPERVIGELAEHGVMSTA
                     WGGDSEFVEISAKFNQNIEELLETVLLVAEIQELKADPTVRAIGTVIEARLDKGKGAV
                     ATLLVQQGTLNVQDPIVVGNTFGRVRAMTNDLGRRVKVAGPSTPVSITGLNEAPMAGD
                     HFAVYEDEKSARAAGEERAKRALMKQRQATQRVSLENLFDTLKAGELKSVNVIIKADV
                     QGSVEALSASLQKIDVEGVKVTIVHSAVGAINESDVTLAEASNAFIVGFNVRPTPQAR
                     QQAEADDVEIRLHSIIYKVIEEMEEAMKGMLDPEFEEKVIGEAVIRETFKVSKVGTIG
                     GFMVINGKVARDSKVRVIRDGVVIYDGELASLKHYKDDVKEVTNGREGGLMIDGYNDI
                     KMDDVIEAYVMEEIKR"
     gene            9584..9934
                     /gene="rbfA"
                     /locus_tag="VYN69_001001"
     CDS             9584..9934
                     /gene="rbfA"
                     /locus_tag="VYN69_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEV1533291.1"
                     /translation="MANHFRTDRVGMEIKREVNEILQKKVRDPRVQGVTITDVQMLGD
                     LSVAKVYYTILSNLASDNQKAQIGLEKATGTIKRELGRNLKLYKIPDLTFVKDESIEY
                     GNKIDEMLRNLDKN"
     gene            10213..10992
                     /locus_tag="VYN69_001002"
     CDS             10213..10992
                     /locus_tag="VYN69_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000840413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533292.1"
                     /translation="MAEQDLAMQVLQQVVKLPVVKVDRSKFLVDKFSKELGPQDIPTL
                     LEQGPTSLLSQEILDRVANACIRDNVLLASGTSVLAGLPGGLAMAITIPADVAQFYGF
                     SLKLAQELGYIYGYEDLWASRDELSEDAQNTLLLYLGVMLGVNGTAALLRVGGITIAK
                     QVMKTVPNKALTKTLWYPILKKVLRIFGVNLTKGGLAKGMGKFIPILGGIISGGLTFA
                     TMKPMGESLQKELSKLVNYSEVQYQEDVETIRKEAEIIEGE"
     gene            10994..11176
                     /locus_tag="VYN69_001003"
     CDS             10994..11176
                     /locus_tag="VYN69_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533293.1"
                     /translation="MGLIKTLAKIYGNYFLTVQGIKVMKTIKKDDHVVVGLGKLFIAD
                     KLMDTARWLIKPEDKK"
     gene            11173..11325
                     /locus_tag="VYN69_001004"
     CDS             11173..11325
                     /locus_tag="VYN69_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000668195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533294.1"
                     /translation="MKFFWGLLAIIFIKPIIGIVKFFWMIISFAVQLLFYKIVFKILD
                     WLFKLI"
     gene            complement(11575..11652)
                     /locus_tag="VYN69_001005"
                     /pseudo
     CDS             complement(11575..11652)
                     /locus_tag="VYN69_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            11683..11916
                     /locus_tag="VYN69_001006"
     CDS             11683..11916
                     /locus_tag="VYN69_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006144975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HEV1533295.1"
                     /translation="MDNIIDVSIPVAEVVDKHPEVLEILVELGFKPLANPLMRNTVGR
                     KVSLKQGSKLAGTPMDKIVRTLEANGYEVIGLD"
     gene            11916..13250
                     /locus_tag="VYN69_001007"
     CDS             11916..13250
                     /locus_tag="VYN69_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000752887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HEV1533296.1"
                     /translation="MADERIHVLRDILLELHNGASPESVQERFDATFAGVSAIEISLM
                     EHELMNSDSGVTFEDVMELCDVHANLFKNAIKGVEVSDTEHPGHPVRVFKEENLALRA
                     ALIRIRRLLDTYELMEDEEMLAEMRKGLVRQMGLVGQFDIHYQRKEELFFPIMERYGH
                     DSPPKVMWGVDDQIRELFQTALTTVKSLPEVSISSVKEAFEAFATEFESMIFKEESIL
                     LMILLESFTQDDWLQIAEESDAYGYAIIRPSEKWVPERQSFIEEKIAEEPVQLDTAEG
                     QVQQVIDTPEGHFTITFTPKEKEAVLDRHSQQAFGNGYLSVEQANLILNHLPMEITFV
                     NKEDIFQYYNDNTPADEMIFKRTPSQVGRNVELCHPPKYLDKVKTIMKRLREGTKDKY
                     EMWFKSESRGKFVHITYAAVHDEDGEFQGVLEYVQDIQPYREIDTDYFRGLE"
     gene            13260..13514
                     /locus_tag="VYN69_001008"
     CDS             13260..13514
                     /locus_tag="VYN69_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1912 family protein"
                     /protein_id="HEV1533297.1"
                     /translation="MSYEQEFMKEFEAWVNTQIMINDMAHKESQKVYEEDQDERAKDA
                     MIRYESRLDAYQFLLGKFENFKVGKGFHDLPEGLFGERNY"
     gene            13610..14005
                     /locus_tag="VYN69_001009"
     CDS             13610..14005
                     /locus_tag="VYN69_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEV1533298.1"
                     /translation="MSKKLNRKKQLRNGLRRAGAFSSTVTKVVDETKKVVKRAEQSAS
                     AAGKAVSKKVEQAVEATKEQAQKVANSVEDFAANLGGLPLDRAKTFYDEGIKSASDFK
                     NWTEKELLALKGIGPATIKKLKENGIKFK"
     gene            14288..14866
                     /locus_tag="VYN69_001010"
     CDS             14288..14866
                     /locus_tag="VYN69_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEV1533299.1"
                     /translation="MKQSFKTNKLYYGFPVFILGYQDQNFGYNITTCSSSYSLGDWVV
                     IGVVARENAAEQIKQYQKFTVNISDETSMLAMEQAGFISHQEKLERLGVHYEISERTQ
                     TPILDACPLVLDCRVDRIVEEDGICHIFAKILERLVAPELLDEKGHFKNQLFAPTYFM
                     GDGYQRVYRYLDKRVDMKGSFIKKARKKDGKN"
     gene            14853..15323
                     /locus_tag="VYN69_001011"
     CDS             14853..15323
                     /locus_tag="VYN69_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1533300.1"
                     /translation="MARTELPDKIETERLVLRVRTVADAEDIFDYASLPEVAYPAGYG
                     IVVKGTDKIVGSVDFNHRHEDDVLEIGYTLHPDYWGRGYVPEAARALIDLAFKDLGLH
                     KIELTCFGYNLQSQRVAEKLGFTLEARIRDRKDAQGNCCDDLRYALLKSEWEVI"
     gene            15323..15901
                     /locus_tag="VYN69_001012"
     CDS             15323..15901
                     /locus_tag="VYN69_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000550018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1533301.1"
                     /translation="MHLFIIGAPASGKMTIGQELSRLTDATLFYNHQAIDFALEIYQD
                     YTEEMWEFVRGITFSFLGASARNQRSVILTDVIDFSNQYQLMYLKQIQNLLDDYHQEI
                     LFVELETSLEECIRRNRTENRLKHKPLKRHIEVSEREILETAETLQLNSQYQPNELHH
                     YFKINNTNLSAEEAAKQIQNKINKIEKGHTHV"
     gene            15894..18545
                     /locus_tag="VYN69_001013"
     CDS             15894..18545
                     /locus_tag="VYN69_001013"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009732359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEV1533302.1"
                     /translation="MSKELSSKYNPAEVEAGRYQKWLDADVFKPSGDQKAKPYSIVIP
                     PPNVTGKLHLGHAWDTTLQDIIIRQKRMQGFDTLWLPGMDHAGIATQAKVEERLRGEG
                     ISRYDLGRESFLTKVWEWKDEYSTTIKEQWGKMGLSVDYSRERFTLDEGLSKAVRKVF
                     VDLYKKGWIYRGEFIINWDPAARTALSDIEVIHKDVEGAFYHMNYMLEDGSRALEVAT
                     TRPETMFGDVAVAVNPEDPRYKDLIGKNVILPIANKLIPIVGDEHADPKFGTGVVKIT
                     PAHDPNDFLVGQRHNLPQVNVMNDDGTMNELAFEFSGMDRFEARKAVVAKLEEIGALV
                     KIEKRVHSVGHSERTGVVVEPRLSTQWFVKMDQLAKNAIANQDTDDKVEFYPPRFNDT
                     FLQWMENVHDWVISRQLWWGHQIPAWYNEAGEIYVGEEAPAGDGWKQDEDVLDTWFSS
                     ALWPFSTMGWPNTETTDFKRYYPNSTLVTGYDIIPFWVSRMIFQGIEFTGKSPFKNAL
                     IHGLIRDEEGRKMSKSLGNGIDPMDVIDKYGTDSLRWFLSNGSAPGQDVRFSYEKMDA
                     SWNFINKIWNISRYILMNNEGLTLDVAHDNVTKVATGEAGNVTDRWILHNLNETIAKV
                     TENFDKFEFGVAGHILYNFIWEEFANWYVELTKEVLYSDNEEEKVITRSVLLYTLDKI
                     LRLLHPIMPFVTEEIFGQISEGSIVTAEYPTVNPAFEDLAAHTGVESLKDLIRAVRNA
                     RAEVNVAPSKPITILVKTSDSDLEAFFNSNVNYIKRFTNPEHLEIASTIPAPELAMSS
                     VITGAEIFLPLVDLLNVEEELARLEKELAKWQKELDMVGKKLSNERFVANAKPEVVQK
                     ERDKQADYQAKYDATVARIDEMKKLVK"
     gene            18615..19370
                     /locus_tag="VYN69_001014"
     CDS             18615..19370
                     /locus_tag="VYN69_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3883 domain-containing protein"
                     /protein_id="HEV1533303.1"
                     /translation="MSKHPHYELLNLIGYGLAKFDKLFIKEFQCSSKSEFYRYVVSLG
                     IAETTGVVKNRMDLFDPYFDNNRKGWWQKAEVYRFRKDLIDMMFGNEDVHSYAEIVKM
                     LLASEGKKTGITIVEKPIVRTKFKRLQETGMEAENYFILHFDKEEKFQGGQLTDARLY
                     GDGYDFQVDVQEYSYLAEVKGIRKSKGRVRLTAKEFEKVKEFQSDFILSLVTNLDDIP
                     KLVLIDNPLKHFEFKKNIIKNEIIEYRSVEDLY"
BASE COUNT         5911 a         3634 c         4426 g         5559 t
ORIGIN      
        1 ttttagagac ttaaccccgt tactgttcat tttaaatatt attggcctgt cttgcttttc
       61 agtgctaaca tacattaaca taaaagagat actcttaaat ataagtaaat gatgatttgg
      121 ttagtcctag aaccaatcaa cttatgttta tcggcttggc agattttatg tttgtcattt
      181 gtttgtatag aggtatttca gaaacagaat tctatcaaca actaatcgct tatataggtg
      241 tattctctgc ttgtttatca cgcttctgct catgtggggc ttgaagtatt ataaaaagta
      301 gctttctatc cttttctgac aggatatttg agtagaaggc tctattaaat tgttagtgaa
      361 ataaatgaat ggaggtattt aatatgaaat acagattatt ttttgttatt ttcttgagta
      421 gtgtgttgga tattctttta gggacatttt tacaaatctc tatcgtatct attgggtggc
      481 ttgttcttta cagtggtttg tttgaagcag gagttttcct tcttgctaat aaaggggtgg
      541 cggtaaaaat caaggaagta gatattcgaa atcgctttaa atttattttt ggaaaaacct
      601 tatggtttca aattcttttg ctcatctttt tgataatcaa actttatctt ggtttggatg
      661 cgaggttgat tttattctat ggacatattt tcattgtctt taatgcctta atgtatctat
      721 tatctagtag tcaggttagc cttaaaaaaa acaaactgtc ttcttaatct tacagcagta
      781 gagcctgaca aggtgaggtc atagtaggga ggatttctat gaaaaagtat caacttctat
      841 tcaaaataag tgcagttttc tcttacttat ttttcgtatt tggtctttct cagatgacgc
      901 ttattattca aaattattgg caattttctt cccagattgg caatttcgtc tggattcaaa
      961 atttcttgag tttgctattt agcggagtca tgatttggat tctggttaag acaggtcatg
     1021 gttatctctt tcacattcca agaaaaaaat ggctttggta ttcgattttg acagtattag
     1081 tggtagtgct ccagatctct tttaacgttc agacagctaa acatgttcag tcaactgctg
     1141 aaggttgggc tgtattgatc ggttatagtg ggaccaactt tgctgagcta ggtatctata
     1201 taactttgtt ctttctgact ccacttatgg aagagctaat ctatagagga ttactgcaac
     1261 acgccttttt taagcattcg agatttggcc ttgatttgct tcttccgtca attttatttg
     1321 ctcttcctca tttttcaagc cttcctagtt tgttagatat cttcatcttt gcaacatctg
     1381 gcatcatctt tgctagtttg acccgctata ccaagagcat ttatccttcc tatgcggtgc
     1441 atgtgatcaa taatattttc gcaacattac catttttgct gactttttta catagggtct
     1501 tggggtaaaa aaacaaaagg cttgcttttc agccatagag gagattatca tgtataaaca
     1561 cttatttttc ctagattcca aaactttaga tcggttgaca ccctatattc tagtcttggc
     1621 ttctgacacc attgctttta atgtttttgt gctaaccttt gtatctgcgg tggtttttaa
     1681 tttcctaaat tccatgctag ctttaatggc tatattcata ggggctggct atgtggtcgg
     1741 attttggtta ctaatactca atgaaaatca aagagcaaac taggaaacta gccgcaggct
     1801 gtacttgagt acggcaaggc gacgttgacg tggtttgaat ttgattttcg aagagtataa
     1861 aatggtttgt tttggaaagg ctagaactaa agaatgacgc gtaggaagtc tgtttggtga
     1921 ggaggatagt tttatggagt tttttgataa atttcatgcc ttgtgttttg gatttttagt
     1981 actaataatt gtcattacag ttccttatac gattaaccat gggggttttt ttcaaaatga
     2041 atctgcattg attcttgtaa gtcttcttgt aacctcgctg agtgttgctt atgctagaaa
     2101 gtttgaaatg atttcttttg ggatgttaag caagaaacaa cttttgcttt tcattgcaat
     2161 ctttcttcta agtgtacttg agacgctggt ttatattcat ttcttcgctg tttcttctgg
     2221 ctcaggggtc caacacttgg cggaagtcag cagaggaatt tccctgtctt tgattttgac
     2281 tacctcagtt tttggcccca tccaggagga actcattttc agaggacttc ttcaaggtgc
     2341 ggtttttgac aattcttggt tagggcttgt gctaacttcc tctctctttt ctttcatgca
     2401 tggaccttct aatgtccctt cgtttatttt ttatctactt gggggtttgt tgctgggctt
     2461 tgcttataaa aagagtcaaa acctatgggt ttctactcta gtccacatgt tttacaacag
     2521 ttggccactc ctatattatt tataaaaatt atgaaaaggt aagcagaaga tggtgcttac
     2581 ctttttcaag ctataatgat acccaagcca atccagaggc ttttctttga gaatcgggtt
     2641 agagtggttg acgaataggc caaaaactag tagaatagta aggaaacttt atacggagga
     2701 aagaaatgga tttgggtgat aatgagctaa cactgactcc catacctggg aaaagtggca
     2761 aggcttatat gggtagctat cctgacggga agcgcatctt tgtaaaaatg aacacctctc
     2821 caatcctacc tggtctagct agagaacaaa ttgctccaca attattatgg agtcgtcgtt
     2881 tggcagatgg gcgtgatatg tgtgctcaag aatggttgac aggcaagata ttgaccccct
     2941 atgatatgaa tcgtaagcaa atcgtcaata ttttaacccg tcttcatcgc tcacgtccgt
     3001 tgatgacaca attgagtcgt ttgggctatg ccatggaaac acctgtagat ttactacagt
     3061 cttggcagga aacggctcca gatgctttgc gtaaaaatca ttttatcagt gaagtgatgg
     3121 ctgatttacg tcagactatt ccaggattta gagaggacca tgcgaccatt gtccatggag
     3181 atgtacgaca tagtaattgg attgagacag atagtggctt gatttattta gtagattggg
     3241 attcggttcg cttgaccgat cgcatgtttg atgtggccca tatgctctgc cattatattt
     3301 cagaacatca gtggaaggaa tggttgacct actacggtta caagtacaat caaacggtat
     3361 taagtaaatt gtattggtat ggtcaattgt cttatttgag tcagatttcc aagtattata
     3421 tgaaccaaga tttagaaaat gtcaatcggg agattcatgg tttgcgtcat ttccgagaca
     3481 agtatggaaa gagaagatga gagttagaaa tcgtaaaggg gcaacagaat tactagaggc
     3541 aaatccccag tatgtggtcc tcaatccctt ggaagccaag gcaaaatggc gggacttgtt
     3601 tggcaatgat aatcccattc atgtggaagt tggaagtgga aagggtgcct ttgtttcagg
     3661 tatggccaag caaaaccctg acatcaacta tatcgggatt gatattcaaa agtctgtttt
     3721 gagctacgct ttggacaagg tgcttgaagt tggagtgcct aacatcaagc tcttgtgggt
     3781 agatggttct gacttaactg actattttga agacggtgag attgatcgct tgtatctgaa
     3841 cttttcagat ccatggccga aaaaacgcca tgaaaagcgt cgtttgacct acaagacctt
     3901 cttggatacc ttcaaacgta tcttgcctga aaatggagaa attcatttca agacggataa
     3961 ccgtggcttg tttgagtaca gtttagtgag cttttctcaa tatggcatga aactcaatgg
     4021 tgtctggtta gatttgcatg ccagtgattt tgaaggcaat gtcatgacag aatacgagca
     4081 aaaattctca aacaaggggc aagttatcta ccgagttgag gcagaatttt aagagatagc
     4141 ctaaaattag gctgtacaag tgcttttgct ttacataagt tggcaaacgt gctatactga
     4201 tagtaagaat atgaaaagtg aggcggggaa atatcttcgc ctcttgctta tgaggaggtg
     4261 gacgcaatcg caacaatcgt agaattagtc agagaagttg tagaacctgt catagaagct
     4321 ccttttgaac tcgtggatat cgagtatgga aagattggca gtgacatgat tctcagtatt
     4381 tttgtagata aacccgaagg aattaccttg aacgacacgg cagacttgac agaaattatc
     4441 agtcctgtcc tagacaccat caagccagat cccttcccag aacaatattt cctagaaatt
     4501 accagtccag gtttggaacg ccctttgaaa accaaggatg ccgtcgctgg agcggttgga
     4561 aaatacatcc atgtcgggct ctaccaagcc atcgataagc aaaaggtctt tgaaggaacc
     4621 ttgttggcct tcgaagagga cgagttgact atggaatata tggacaagac gcgtaagaaa
     4681 accgtccaaa ttccatacag tttagtatca aaagcacgtt tagcagttaa attatagaaa
     4741 aagaaaggat agcttttgag gattcaaaag tgaagaaaac atgagtaaag aaatgctaga
     4801 ggccttccgc attttggaag aagacaaggg aatcaaaaaa gaagatatca tcgacgcagt
     4861 agtagagtcg cttcgttccg cttatcgcag acgctatggt cagtcagaca gcgtagctat
     4921 tgacttcaac gaaaaaacag gtgactttac agtttatact gtccgtgaag ttgttgatga
     4981 agtatttgat agccgtttgg aaatcagctt gaaagatgct cttgccatta attcagctta
     5041 tgaacttgga gacaaaatca agtttgaaga agcaccagct gagtttggtc gtgtagcagc
     5101 ccagtctgcc aaacaaacca tcatggaaaa aatgcgcaag caaacacgtg ccatcactta
     5161 caatacttac aaagaacatg agcaagaaat catgtctggt acagtagaac gctttgacaa
     5221 ccgctttatc tatgtcaacc ttggtagcat cgaagcccaa ttgtcaaaac aagaccaaat
     5281 tcctggagaa gtttttgctt ctcatgatcg tatcgaagtt tatgtttaca aggttgaaga
     5341 caaccctcgt ggtgtgaacg tctttgttag ccgtagtcat ccagaaatga tcaaacgttt
     5401 aatggagcaa gaaattccag aagtttatga tggaactgtt gaaatcatga gcgtggctcg
     5461 tgaagcaggt gaccgtacga aggttgctgt tcgtagccac aatccaaacg tggatgctat
     5521 cggtacaatc gttggacgtg gtggtgctaa tatcaagaag attactagca aattccaccc
     5581 agctcgttac gatgctaaaa atgaccgcat ggtaccaatc gaagaaaata tcgatgttat
     5641 cgagtgggta gcagatccag ctgaatttat ctacaatgcc atcgctcctg ctgaggttga
     5701 ccaagttatc tttgatgaaa acgacagcaa acgtgccttg gtggttgttc cagataacaa
     5761 gctttctctt gccattggtc gtcgtggaca aaacgtgcgc ttggcggctc acttgactgg
     5821 ttaccgtatc gatatcaagt ctgctagcga atttgaagcc atggaagacg ctgcttcagt
     5881 agagttggaa gtagaaaacg atactgtaga agaataaaag ctgctagagg agggaaagat
     5941 gaaaacaaga aaaatccctt tgcgcaagtc tgttgtgtct aacgaagtga ttgataagcg
     6001 tgatttgctc cgcattgtca agaacaagga aggacaagtc tttattgatc ctacgggcaa
     6061 ggccaatggc cgcggcgctt atatcaaact agacaatgca gaagccctag aggcgaaaaa
     6121 gaagaaggtc tttaaccgca gctttagcat ggaagtggaa gaaagctttt atgacgagtt
     6181 gatcgcttat gtggatcaca aagtgaaaag aagagagttg ggacttgaat aagcaaaaga
     6241 taagtaatct cttggggctt gctcagcgag cagggcgcat catatcgggt gaagaattgg
     6301 tggtcaaggc cattcaagac ggcaaggcca agttggtctt tctagctcat gatgctggac
     6361 ccaatctgac caagaagatt caagataaaa gtcattatta tcaagtagaa attgtaaccg
     6421 tgttttcaac actggaatta agcatagcag tcgggaaatc gagaaaggtt ttggctgtaa
     6481 cagatgctgg atttacaaag aaaatgaggt ctcttatgga atagaagagg aggacatgat
     6541 ttgtctaaga aaagattgta cgaaatcgca aaagaacttg gaaaagaaag taaagaagtt
     6601 gtagcgcgtg caaaagagtt gggcttggat gtgaaaagcc actcatcaag tgtggaagaa
     6661 gctgtcgctg caaaaattgc tgccagcttt aagcctgcag ctgctccgaa agtagaagca
     6721 aaacctgcag caccaaaagt aagtgcagaa aagaaagccg aaaaatctga gccagctaaa
     6781 ccagctgtag ctaaggaaga ggcaaaaccg gctgagccag ttgctccgaa aacagaaaaa
     6841 gtagcagcga aaccgcaaag tcgtaatttc aaggctgagc gtgaagcccg tgctaaagag
     6901 caggcagaac gacgcaagca aaataagggc aataaccgtg accaacaaca aaacggaaac
     6961 cgtcagaaaa acgacggccg taatggtgga aaacaaggtc aaagcaaccg cgacaatcgt
     7021 cgctttaatg accaagctaa gaagcagcaa ggtcagcaaa aacgtagaaa tgagcgccgt
     7081 cagcaagagg ataaacgttc aaatcaagcg gctccacgta ttgactttaa agcccgtgca
     7141 gcagccctaa aagcagagca aaatgcagag tacgctcgtt caagtgagga acgcttcaag
     7201 cagtatcagg ctgctaaaga agccttggct caagctaaca aacgcaagga accagaggaa
     7261 atctttgaag aagcggctaa gttagctgaa caagcacagc aagttcaagc agtggttgaa
     7321 gtcgtccctg agaaaaaaga acctgcagtg gatacacgtc gtaaaaaaca agctcgacca
     7381 gacaaaaatc gtgacgatta tgatcatgaa gaagatggtc ctagaaaaca acaaaagaat
     7441 cgaagtagtc aaaatcaagt gagaaatcaa aagaatagta actggaataa caacaaaaag
     7501 aacaaaaaag gcaataacaa gaacaaccgt aatcagactc caaaacctgt tacggagcgt
     7561 aaattccatg aattgccaac agaatttgaa tatacagatg gtatgaccgt tgcggaaatc
     7621 gcaaaacgta tcaaacgtga accagctgaa atcgttaaga aacttttcat gatgggtgtc
     7681 atggccacac aaaaccaatc cttggatggg gaaacaattg aactcctcat ggtggattac
     7741 ggtatcgaag ccaaacaaaa ggttgaagtg gataatgctg acatcgaacg tttctttgtc
     7801 gaagatggtt atctcaatga agatgaattg gttgagcgtc caccagttgt tactatcatg
     7861 ggacacgttg accacggtaa aacaaccctt ttggatactc ttcgtaactc acgtgttgcg
     7921 acaggtgaag caggtggtat tactcagcat atcggtgcct accaaatcgt ggaaaatggt
     7981 aagaagatta ccttccttga tacaccagga cacgcggcct ttacatcaat gcgtgcgcgt
     8041 ggtgcttctg ttaccgatat tacgatcttg gtcgtagcgg cagatgacgg ggttatgcct
     8101 cagactattg aagccatcaa ccactcaaaa gcagctaacg ttccaatcat cgtagccatt
     8161 aacaagattg ataaaccagg tgctaaccca gaacgcgtta tcggtgaatt ggcagagcat
     8221 ggtgtgatgt caaccgcttg gggtggagat tctgaatttg ttgaaatctc agctaaattc
     8281 aaccaaaata tcgaagaatt gttggaaaca gtccttcttg tagctgaaat ccaagaactc
     8341 aaggcagacc caacagtgcg tgcaatcggt acggttatcg aagcgcgctt ggataaagga
     8401 aaaggtgcgg tcgcaaccct tcttgtacaa caaggtacct tgaatgttca agacccaatc
     8461 gttgtcggaa ataccttcgg tcgtgtccgt gctatgacca acgaccttgg tcgtcgtgtt
     8521 aaagttgctg gaccatcaac accagtctct atcacaggtt tgaacgaagc accgatggcg
     8581 ggtgaccact ttgccgttta cgaggatgaa aaatctgcgc gtgcagcagg tgaagagcgt
     8641 gccaaacgtg ccctcatgaa acaacgtcaa gctacccaac gtgttagcct tgaaaacctc
     8701 tttgataccc ttaaagctgg ggaactcaaa tctgttaatg ttatcatcaa ggctgatgta
     8761 caaggttctg ttgaagccct ttctgcctca cttcaaaaga ttgacgtgga aggtgtcaaa
     8821 gtgactatcg tccactcagc ggtcggtgct atcaacgaat cagacgtgac ccttgccgaa
     8881 gcttcaaatg cctttatcgt tggtttcaac gtacgcccta caccacaagc tcgtcaacaa
     8941 gcagaagctg acgatgtgga aatccgtctt cacagcatta tctacaaggt tatcgaagag
     9001 atggaagaag ctatgaaagg gatgcttgat ccagaatttg aagaaaaagt tattggtgaa
     9061 gcggttatcc gtgaaacctt caaggtgtct aaagtgggaa ctatcggtgg atttatggtt
     9121 atcaacggta aggttgcccg tgactctaaa gtccgtgtta tccgtgatgg tgtcgttatc
     9181 tatgatggtg aactcgcaag cttgaaacac tataaagacg acgttaagga agttacaaac
     9241 ggccgtgaag gtggattgat gatcgacggc tacaatgata ttaagatgga tgatgtgatt
     9301 gaggcgtatg tcatggaaga aatcaagaga taagattttt tgctcctttc ttaggtggtg
     9361 agggacgcaa gcaaaccgat ggtttcattg cttatttttg agcctagggt ctcaaaaatc
     9421 ccctgtgatg ggactgataa atcagttcca tcactttcac cacggcgaaa gaagcagatg
     9481 acttcaaatt gaacttcgtt tcaatttaaa ctgaaaatca agaagtttaa aatagctagg
     9541 tctgctggcc tagcttttgg ttcaaagtag agaaaggaat atcatggcaa atcatttccg
     9601 tacagatcgt gtgggcatgg aaatcaagcg tgaagtcaat gagattttgc aaaagaaagt
     9661 ccgtgatcca cgtgtccaag gtgtgaccat cacagatgtt cagatgctgg gtgacttgtc
     9721 tgttgccaag gtttattaca ccattttgag taaccttgct tcggataacc aaaaagccca
     9781 aatcgggctt gaaaaagcaa ctggtaccat caaacgtgaa cttggtcgca atttgaaatt
     9841 gtacaaaatc ccagatttga ccttcgtcaa agacgagtcc atcgagtatg gaaacaagat
     9901 tgacgagatg ctacgcaatc tggataagaa ctaaagaaga ggggttgccc ctcttttttg
     9961 gtggaggaaa ataggttgaa tttgaaatgg aaaaatattc ttttaaaata tattgaaact
    10021 agaatagtac gcctctactt ctaaaacatt gttagaaatc gatttgactg tcctgatcgg
    10081 tttgtcctgt tcttgtttca ttttactata aaaaagggat tctgtatttt ttaatgttat
    10141 ctaattagga aatgcttttt ttgtaggaaa tataatatga taaggtgtaa aaaagaaata
    10201 aggagtttgt atatggctga acaagactta gctatgcaag tattacaaca agtggtaaaa
    10261 ctaccagttg ttaaggttga tcgttcgaaa tttttagtgg ataagttttc caaagaattg
    10321 ggtccacagg acattcctac cttattggaa caaggtccaa cgtctctctt atctcaagaa
    10381 atcttagatc gagtggctaa tgcctgtatt agggataatg ttttattagc gagtgggact
    10441 tctgttttgg caggattacc tggagggctt gctatggcaa ttaccattcc agctgatgtg
    10501 gctcaatttt atggtttctc tctgaaattg gcccaagaat taggttatat ttatggttat
    10561 gaggatcttt gggcttcacg cgatgagttg agtgaagatg ctcaaaatac cctcttgctt
    10621 tatctaggcg tcatgttggg ggtgaatgga actgctgctt tgctacgtgt tggtggtata
    10681 acaattgcca aacaggtaat gaaaacagta cctaataaag ctttaacaaa gacgctttgg
    10741 tatcctattt tgaagaaagt cttaagaata tttggtgtga atcttaccaa gggagggctg
    10801 gccaaaggaa tggggaaatt tattcctatc ttgggtggta tcatttcagg tggtttaacc
    10861 tttgcaacta tgaaaccgat gggtgaaagc ttgcagaaag aattgtccaa gctagttaac
    10921 tatagtgaag ttcaatatca agaagatgtt gaaacaatcc gaaaagaggc tgaaatcatc
    10981 gaaggagagt aatatgggac tcataaaaac tctagctaaa atttatggta attacttttt
    11041 gaccgtgcaa ggtataaaag taatgaaaac gataaagaaa gatgaccatg tcgttgttgg
    11101 tctgggaaaa ctctttattg cagataagct aatggatacg gctcggtggc tcattaagcc
    11161 agaggataaa aaatgaaatt tttctgggga cttcttgcca ttatttttat caaaccgatt
    11221 attgggattg tgaaattctt ttggatgatc atctcttttg cagtccaatt gctgttttac
    11281 aagatagtgt ttaagatatt ggattggctc tttaaactta tctagatggt aatccaagtc
    11341 gcagagaact agcaggaact ccactgctag ttttttattc tctttccata tggtataata
    11401 taagcagtaa aatcatttta tactcttcga aaatctcttc aaaccacgtc agcttcacct
    11461 tgctgtatat atgttactga cttcgtcagt tctatccaca acctcaaaac ggtgttttga
    11521 gctgacttcg tcagttctat ccacaacctc aaaacggtgt tttgagctga cttcgtcagt
    11581 tctatccaca acctcaaagc actgttttga gcagtctgca gctagcttcc tagtttgctc
    11641 tttgattttc attgagtatt agaacataca atggaggtcg tcatggacaa tatcatcgat
    11701 gtgtcaattc ctgttgcaga agtggtggac aagcatccag aagtcttgga aattctagtg
    11761 gagttgggtt ttaaacccct tgccaatccc ttaatgcgca atacagttgg tcgtaaagta
    11821 tcacttaaac agggttctaa gctagcagga actcctatgg acaagattgt acgcacactg
    11881 gaagcgaatg gctacgaagt gattggatta gactaatggc agatgaacgg attcatgtcc
    11941 tacgggatat tttgttagaa ttgcacaatg gcgcctctcc tgagtcggtt caagagcgct
    12001 ttgatgcgac ctttgcgggt gtctcagcca tcgagatttc ccttatggag cacgagctga
    12061 tgaactcaga ctcaggtgtc acctttgaag atgttatgga actctgtgat gtccatgcca
    12121 atctttttaa aaatgctatc aaaggtgtcg aagtttcaga tactgagcat ccaggtcacc
    12181 cagttcgtgt cttcaaagaa gaaaatctgg ctctccgtgc ggccttgatt cgcattcgta
    12241 gattgttaga tacctatgag ttgatggaag acgaggaaat gcttgcggag atgcgcaagg
    12301 gcttggtgcg tcagatgggg cttgtggggc aatttgacat ccattaccaa cgtaaggaag
    12361 aactcttctt tcctatcatg gagcgctatg gacacgattc acctcccaaa gttatgtggg
    12421 gagtggatga tcagattagg gaactctttc aaacagctct aacgacagtc aagtcactac
    12481 cagaagtgtc aattagcagt gtaaaggaag cttttgaagc ttttgcgaca gagtttgaaa
    12541 gtatgatttt caaggaagag tccatcctcc tcatgattct ccttgagtct tttactcagg
    12601 atgactggct tcagattgcg gaggagagcg atgcctatgg ctatgccatc atccgtccgt
    12661 cagagaaatg ggtgccagaa cgacagagct ttattgagga aaagattgca gaggagcctg
    12721 tacagctaga tacggcagaa ggtcaagttc aacaagtcat agatacgcca gaaggccatt
    12781 ttaccattac ctttacccct aaggaaaagg aagctgtgct ggaccgccat agtcaacagg
    12841 cttttggtaa tggctatctt tcagtcgagc aggccaatct catcctcaat catctcccta
    12901 tggagattac ctttgtcaat aaagaagata ttttccagta ttacaatgac aatacgccag
    12961 ctgatgagat gattttcaaa cggacgccgt ctcaagtcgg gcgcaatgtc gaactctgcc
    13021 atccgcctaa gtacttggac aaggtcaaaa ctatcatgaa gaggcttcgt gaggggacca
    13081 aggacaagta tgaaatgtgg ttcaagtctg agtcgcgagg taagtttgtc cacatcacct
    13141 atgctgcagt acacgatgaa gacggagaat tccaaggagt gttggagtat gttcaggata
    13201 tccagcccta ccgtgagatt gatacggact attttcgtgg attagaataa ggagaaaaaa
    13261 tgagttacga acaagaattt atgaaggaat ttgaagcttg ggtcaatacc caaatcatga
    13321 ttaacgacat ggcgcacaag gaaagtcaaa aagtttacga agaagaccag gacgagcgtg
    13381 ccaaagatgc catgattcgc tacgagagtc gcttggatgc ttatcagttc ttgcttggta
    13441 agtttgaaaa cttcaaagta ggcaagggat tccatgattt gccagaaggc ttgtttggtg
    13501 agcgaaatta ttaaacgaga aagattcttg atttttcact aaaatcttga tagaatgttt
    13561 atgttaaatc cttgtcagag cagggatttt ttattgaaag gattttatca tgtcaaagaa
    13621 actcaatcgt aaaaaacaat tacgaaatgg cctccgtcgc gcaggtgcct tttcaagtac
    13681 tgtgactaag gttgtagatg agacaaaaaa agtcgtgaag cgtgcagaac agtcagcaag
    13741 cgcagctggt aaggctgttt ctaaaaaagt tgaacaagca gtagaagcta ccaaagagca
    13801 agctcaaaaa gtagctaatt ctgtagaaga ttttgcagca aacttgggtg gacttccact
    13861 tgatcgtgcc aagactttct atgatgaagg aatcaagtct gcttcagatt tcaaaaactg
    13921 gactgaaaaa gaactccttg ccttgaaagg aatcggccca gctaccatca agaaattgaa
    13981 agaaaatggc atcaagttca agtaattttt cttgagcctt gcatttccga gaaaaacttg
    14041 ctacaataga gctattagag gtgttttgaa tcccacattt tacagaaagt ggcggcgctg
    14101 agaagtccac aaatgtgtca aaactggttg ctaatgactg aaaaaatgaa atatttctgt
    14161 ctttttattt taaagactaa agatgcgggc ttggcccgaa attgggtggt accgcggata
    14221 aatacattcg tccctgtcat gtagatggca gggcttttct tttgtgtctt agtcaaagga
    14281 ggttgttata aaacaatcat ttaaaactaa taaactatat tatggttttc ctgtttttat
    14341 tttaggatat caagatcaga attttggcta taacatcacg acctgtagtt cctcgtatag
    14401 tctaggagat tgggttgtga ttggagtcgt tgcgagagag aatgccgcag agcagatcaa
    14461 acagtatcaa aaatttactg tgaatatttc tgatgaaact tctatgcttg cgatggagca
    14521 ggctggtttt atcagtcatc aggagaaatt ggaacgtttg ggagtgcatt atgaaatttc
    14581 tgaacgaact cagactccta ttttagacgc ctgtccactt gttttagatt gtcgggtaga
    14641 taggattgtt gaggaagacg gtatttgcca catctttgcc aagattcttg agcgacttgt
    14701 tgccccagaa ctcctggatg aaaagggaca ttttaaaaat caactgtttg ccccaaccta
    14761 tttcatggga gatggatatc agcgcgttta tcgctatctg gataagcgtg tagatatgaa
    14821 gggcagtttc atcaaaaaag cgaggaagaa ggatggcaag aactgagctg ccagataaaa
    14881 tcgaaacaga acgtctcgtt ttacgagtcc gtactgtggc ggatgctgag gatatctttg
    14941 actatgctag tttgccagag gtcgcctatc cagcaggcta cgggattgtc gtcaaaggaa
    15001 cggataagat cgttggctct gtcgatttca accatcgcca tgaagatgat gtgctggaaa
    15061 tcggctatac cttacaccca gactattggg ggcgaggtta tgtgccagaa gctgcgcgtg
    15121 ccttgattga cttagccttt aaagatttgg gtcttcacaa gattgaacta acttgctttg
    15181 gatataacct tcaaagtcaa cgagtcgcgg aaaagcttgg ctttaccctc gaagctcgca
    15241 taagagaccg taaagatgct caaggcaact gctgtgatga tttgagatat gccttgctga
    15301 agagtgagtg ggaggtgatt tgatgcatct tttcatcatt ggtgctccag catcaggaaa
    15361 aatgaccatt ggtcaagaat tatctcgact gacggatgct accctatttt ataaccatca
    15421 agccattgat tttgcactag aaatctatca ggactataca gaggaaatgt gggaatttgt
    15481 tcgtggaatc actttttcat tccttggagc aagtgcaaga aatcagcgat cagtaatttt
    15541 aacagatgta attgattttt caaatcagta tcaactgatg tatttaaagc aaattcagaa
    15601 tttgttggat gattatcatc aagagattct ttttgttgag ttggaaacaa gtcttgaaga
    15661 gtgcatacgt cgaaatcgaa cggagaatcg attaaagcac aaacccttaa aacgacatat
    15721 tgaggtatct gaaagagaaa ttttagagac cgctgaaaca cttcaattaa attcccaata
    15781 tcaaccgaat gagttgcacc actactttaa aataaataat acgaatttgt ctgcagaaga
    15841 agctgctaag caaattcaaa ataaaataaa caaaatagag aaaggacaca cacatgtcta
    15901 aagaactttc atctaaatac aatccagccg aggttgaggc tggtcgttac caaaaatggc
    15961 ttgatgctga tgttttcaag ccttcaggcg atcaaaaggc taagccttat tcaatcgtta
    16021 ttccaccacc aaacgttaca ggtaaacttc accttggtca cgcttgggat acaactttgc
    16081 aagatattat catccgtcaa aaacgcatgc aaggttttga taccctttgg cttcctggga
    16141 tggaccacgc agggattgcc actcaggcta aggtagagga gcgcttgcgt ggtgagggca
    16201 tttcccgcta tgaccttggt cgtgagtctt tcttgacgaa agtctgggaa tggaaagacg
    16261 aatattccac tactatcaag gaacaatggg gcaagatggg gctctctgta gactactctc
    16321 gtgagcgttt cactcttgac gaaggtttgt caaaagctgt tcgtaaggtc tttgtggacc
    16381 tttacaagaa aggctggatc taccgtggtg agtttatcat caactgggac ccagcagctc
    16441 gcacagccct ttctgatatc gaggttattc acaaggacgt cgaaggtgcc ttctatcaca
    16501 tgaattacat gctggaagat ggttcacgcg cccttgaagt tgctacaact cgtcctgaga
    16561 ccatgtttgg ggacgttgcg gttgcggtca acccagaaga cccgcgctac aaggacttga
    16621 ttggtaaaaa tgtcatcctt ccaattgcta ataaattaat cccaatcgtt ggtgatgagc
    16681 acgcagatcc taagtttggt actggtgtcg tgaaaatcac acctgcccac gatccaaatg
    16741 acttcttggt tggccaacgt cataacttgc cacaagtcaa cgtcatgaac gacgacggaa
    16801 ctatgaacga gcttgccttt gaattttcag gcatggatcg ttttgaagct cgtaaggcag
    16861 tcgttgctaa gttggaagaa atcggtgccc ttgtcaaaat cgaaaaacgt gtccattcag
    16921 tcggtcactc agaacggaca ggtgtcgtag ttgagccacg cttgtctact caatggttcg
    16981 tcaagatgga ccaattggcc aagaacgcca ttgccaacca agacacagat gacaaggtgg
    17041 aattttaccc accgcgcttc aacgacacct tcctgcaatg gatggaaaat gtccacgact
    17101 gggtgatttc ccgtcagctc tggtggggtc accaaatccc tgcttggtat aatgaagcag
    17161 gcgaaatcta tgtcggcgag gaagccccag cgggcgatgg ctggaaacag gacgaagatg
    17221 tccttgacac ttggttcagc tctgccctct ggcccttctc aaccatgggc tggcctaata
    17281 ccgaaactac tgactttaaa cgctactatc caaattcaac actcgtaaca ggctatgata
    17341 ttattccttt ctgggtatca cgcatgattt ttcaaggaat tgaatttaca ggaaagagcc
    17401 catttaaaaa tgctcttatc catggtctaa ttcgtgatga agaaggacgt aagatgtcta
    17461 aatctcttgg aaatggcatt gacccaatgg atgttattga taaatatggt acagatagtc
    17521 ttcgttggtt cttgtcaaac ggctcagctc ctggtcaaga cgttcgtttc tcttacgaga
    17581 aaatggatgc ttcatggaac tttattaaca aaatttggaa catttctcgc tatatcctca
    17641 tgaacaatga aggtttgacg ctggatgtgg cgcatgacaa tgtcacaaaa gttgcaacag
    17701 gtgaggctgg taatgtgacg gaccgctgga ttctccacaa tctcaacgaa accattgcaa
    17761 aagttactga aaactttgat aagttcgagt ttggtgtggc tggtcatatc ctttacaact
    17821 tcatctggga agaatttgcc aactggtatg ttgagttgac caaggaagtc ctttatagcg
    17881 ataacgaaga agagaaagtc atcacacgtt ctgttctact atacactttg gacaagatcc
    17941 ttcgtctcct tcacccaatc atgccatttg tgacagagga aatctttgga caaatctcag
    18001 aaggatctat cgttacagca gaatacccaa ctgtcaaccc agcctttgaa gaccttgctg
    18061 ctcacactgg tgtggaaagc cttaaagact tgatccgtgc cgttcgtaat gcgcgtgcgg
    18121 aagtaaacgt tgctccaagc aagcctatca ctatccttgt taagacaagc gatagcgact
    18181 tggaagcctt ctttaacagc aatgtcaact acatcaaacg tttcacaaat cctgaacact
    18241 tggaaatcgc atcaaccatc cctgcacctg aactggctat gtcaagcgtc atcacaggag
    18301 ccgaaatctt cttgccactg gtagacctct tgaatgtcga agaagaattg gcacgtctgg
    18361 aaaaagaact tgccaaatgg cagaaagaac tcgacatggt tggcaaaaaa ctcagcaacg
    18421 aacgcttcgt cgccaacgct aaaccagaag ttgtccaaaa agaacgcgac aagcaagcag
    18481 attaccaagc taagtatgat gcgaccgtag cacgtattga tgagatgaag aagttggtga
    18541 aataaacaca gaaacacggt gatgagccgt gttttttggt ataatgaaat caatatctat
    18601 tgatggagaa agtgatgtcc aaacaccctc attatgaatt gttaaattta attggctacg
    18661 gtcttgccaa gtttgacaag ctttttataa aagaatttca atgctctagc aagtcggagt
    18721 tttatcgcta tgtggtttct ctgggaattg ctgaaacaac tggagttgta aaaaatagaa
    18781 tggatttatt tgatccttat tttgacaata atcgaaaagg ttggtggcag aaagctgaag
    18841 tttatcgttt tcgtaaagat ttaattgata tgatgtttgg aaatgaagat gttcatagtt
    18901 atgctgaaat agttaaaatg ttacttgcca gtgaagggaa gaaaacaggc ataaccatcg
    18961 ttgaaaaacc aatagttcga actaaattca aacgtctaca ggaaacggga atggaggctg
    19021 agaattattt tatccttcat tttgataaag aagaaaaatt ccaaggtgga cagttaaccg
    19081 atgcccgtct ttatggtgat ggatatgact ttcaggtaga tgtccaagag tattcttacc
    19141 ttgccgaagt aaaaggtatc cgaaaatcta aggggcgtgt gcgtttaact gcaaaagaat
    19201 ttgaaaaagt caaggagttt caatcggatt ttattttatc cttggtcaca aatctagatg
    19261 atattccaaa gttagtgtta atcgataatc ctttaaaaca ttttgagttt aaaaagaata
    19321 ttatcaaaaa tgaaatcatt gaatatagaa gcgtagagga tttgtattag ttccccctta
    19381 acttttccag ttgaaggaac gagaagtctt gacaggtcaa cgacttaatg aactggaaat
    19441 caacggtatt cgtttgacta agttttaaga tggcgaaatc ggtattgaat ttatcagtag
    19501 tgctgattat aactctttta ttatttacat
//
LOCUS       DAVLVP010000030        18816 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.030, whole genome shotgun sequence.
ACCESSION   DAVLVP010000030 DAVLVP010000000
VERSION     DAVLVP010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18816)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18816)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18816
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.030"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..2394
                     /locus_tag="VYN69_001015"
     CDS             <1..2394
                     /locus_tag="VYN69_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000473013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease"
                     /protein_id="HEV1533304.1"
                     /translation="NKLNKEHHLNTKEVLSVTAMNNNEFITNLDEANKIIVHYADGTK
                     DYFNLSSSSEGLSNVKEYTITDLGIKYTPNIVQKDNTTLVNDIKSILESVELQSQTMY
                     QHLNRLGDYRVNAIKDLYLEESFTDVKENLTNLITKLVQNEEHQLNDSPAARQMIRDK
                     VEKNKAALLLGLTYLNRYYGVKFGDVNIKELMLFKPDFYGEKVSVLDRLIEIGSKENN
                     IKGSRTFDAFGQVLAKYTKSGNLDAFLNYNRQLFTNIDNMNDWFIDATEDHVYIAERA
                     SEVEEIKNSKHRAFDNLKRSHLRNTILPLLNIDKAHLYLISNYNAIAFGSAERLGKKS
                     LEDIKDIVNKAADGYRNYYDFWYRLASDNVKQRLLRDAVIPIWEGYNAPGGWVEKYGR
                     YNTDKVYTPLREFFGPMDKYYNYNGTGAYAAIYPNSDDIRTDVKYVHLEMVGEYGISV
                     YTHETTHVNDRAIYLGGFGHREGTDAEAYAQGMLQTPVTGSGFDEFGSLGINMVFKRK
                     NDGNQWYITDPKTLKTREDINRYMKGYNDTLTLLDEIEAESVISQQNKDLNSAWFKKI
                     DREYRDNNKLNQWDKIRNLSQEEKNELNIQSVNDLVDQQLMTNRNPGNGIYKPEAISY
                     NDQSPYVGVRMMTGIYGGNTSKGAPGAVSFKHNAFRLWGYYGYENGFLGYASNKYKQQ
                     SKTDGESVLSDEYIIKKISNNTFNTIEEFKKAYFKEVKDKATKGLTTFEVNGSSVSSY
                     DDLLTLFKEAVKKDAETLKQEANGNKTVSMNNTVKLKEAVYKKLLQQTDSFKTSIFK"
     gene            2455..4176
                     /gene="pabB"
                     /locus_tag="VYN69_001016"
     CDS             2455..4176
                     /gene="pabB"
                     /locus_tag="VYN69_001016"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000554849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HEV1533305.1"
                     /translation="MHRKTVIDFRTLGERYTFTKPIKELKTRNVEEVADLLAQVESYQ
                     EQGYYVVGYVSYEAAPAFEEKLAVHKDPLLGEYLLYFTVHDSVETSSIPLTYEDIDLP
                     SNWQEVTSAADYEKAIAQIHHHLRQGDTYQVNYTVQLKQKLNANPFAIYNRMVVEQEA
                     GYNAYVEHDEMAVISMSPELFFEQNDRELTTRPMKGTTQRGVTDQEDLEQASWLEQDP
                     KNRSENMMIVDLLRNDMNRISEVGSEHVERLCQVEQYSTVWQMTSTIKSQLREDVDLV
                     EIFRSLFPCGSITGAPKIATMEIIKDLEPQPRGVYCGTIGLLLPNGRRIFNVAIRTIQ
                     LHKGQAIYGVGGGITWDSTWESEYREVHQKAAVLYRKQARFQLITTGEISQKNLLFED
                     QHLERLRKASRYFAFPFDAEDLGHKIEEECQDCEANRDYRLRISLSKSGEIEVNRQVL
                     TPLSTSFCQAQVCLQEAALNQSFTYFKTTHRPHLSLGEQEKIYHNKSGELLETSIGNL
                     VLKIAGKLYTPPIRLGILPGIYRQYLLETGQVEEKVLTLADLAQAEAIYGCNAVRGLY
                     ELQVIGL"
     gene            4296..5822
                     /locus_tag="VYN69_001017"
     CDS             4296..5822
                     /locus_tag="VYN69_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1533306.1"
                     /translation="MKSKIVLGASLAIATLSLVSLVEIEGLSPFLIENVSANTHSANK
                     VINHKVSIYLENADEGKGLTVNFSTDSVSPNLFDEFEKKSGITITTMLVNAKTGEVVE
                     KRLTPSVFLRSNDLTSGTISSFIFSEYPDGEYKYVVSKGDFIDPKTQFKHQYRGESPV
                     FRIRNRKYVELGTTDKKLDERRDNSVYKDGVVEHKVNLSLTSYQGGNGVTAIFSTDSV
                     NSNLLNSFGEKAKKVLIRSKLINVKTGEVIDETFSPKVSLTSKILKSGSTAVFYFIDL
                     TDGEYKYVAYESQQYTDPQTTLTHQYRGESPIFSIKDGKFSGLVSASKPDENPKPTPK
                     PDEKPKPSAPQQEKPKPTVQSGWVGSSYYQNGKKVTSKWIFDKKYNSYFYLDASGNYV
                     QNAWVGNYYLKSGGYMAKSEWIYDKNYGSYYYLTSEGSYARNTWSGNYYLKSNGKMAK
                     SEWVYDSNYKSYYYLTSEGSYARNTWVGNYYLKSNGKMAVNERTPDGYRVDGSGKWVK
                     "
     gene            5909..6835
                     /locus_tag="VYN69_001018"
     CDS             5909..6835
                     /locus_tag="VYN69_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1533307.1"
                     /translation="MSTIDKSGVLPFSAQTVQADIQTLTPMAKYYIILPKDAEIYKTW
                     RGTVNIPIIDATKTTPELSYFKEDHRNYIANENKSGANYIEWKGTVEEFKEAIKKLTD
                     KKSTTATPKKDEKPTPKPDEKPKPTPTVQSGWVGSSYYQDGKKVISKWIFDKKYNSYF
                     YLDASGNYVQNAWVGNYYLKSGGYMAKGEWVYDATYQAWYYLTSDGSYAYSTWQGNYY
                     LKSDGKMAVNEWVDGGRYYVGADGVWKEGQASTVSSSNDSNSEYSAALGKAKSYNSLF
                     HMSKKRMYRQLTSDFDKFSNDAAQYAIDHLDN"
     gene            6962..7921
                     /locus_tag="VYN69_001019"
     CDS             6962..7921
                     /locus_tag="VYN69_001019"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002919069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEV1533308.1"
                     /translation="MSQKIIGIDLGGTSIKFAILTTAGEIQGKWSIKTNILDEGSHIV
                     DDMIESIQHRLDLLGLAAADFQGIGMGSPGVVDRDKGTVIGAYNLNWKTLQPIKQKIE
                     KALGIPFFIDNDANVAALGERWMGAGDNQPDVVFMTLGTGVGGGIVAEGKLLHGVAGA
                     AGELGHITVDFDQPISCTCGKKGCLETVASATGIVNLTRRYADEYEGDAALKRLIDNG
                     EEVTAKTVFDLAKEGDDLALIVYRNFSRYLGIACANIGSILNPSTIVIGGGVSAAGEF
                     LLQGVQKVYDENSFPQVRTSTKLALATLGNDAGVIGAASLVLQ"
     gene            8018..8857
                     /locus_tag="VYN69_001020"
     CDS             8018..8857
                     /locus_tag="VYN69_001020"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003011065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEV1533309.1"
                     /translation="MTKADTIFKENIERILKEGVFSEQARPKYKDGTVANSKYVTGAF
                     SEYDLSKGEFPITTLRPIAIKSAIKEVLWIYQDQSNSLEVLNDKYNVHYWNDWEVGDT
                     GTIGERYGAVVKKHDIINKLLKQLETNPWNRRNIISLWDYQAFEETDGLLPCAFQTMF
                     DVRRVDGEIYLDATLIQRSNDMLVAHHINAMQYVALQMMIAKHFGWKVGKFFYFINNL
                     HIYDNQFEQAQELLRREPSNCQPRLVLNVPDGTNFFDIKAEDFELVDYDPVKPQLKFD
                     LAI"
     gene            complement(8905..9075)
                     /locus_tag="VYN69_001021"
     CDS             complement(8905..9075)
                     /locus_tag="VYN69_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEV1533310.1"
                     /translation="MAKGFAKGLVTGVAGTVAAVAGAVYAFKKKVIEPEEQKAAFIEE
                     NRKKAARRRVSR"
     gene            9196..10080
                     /gene="miaA"
                     /locus_tag="VYN69_001022"
     CDS             9196..10080
                     /gene="miaA"
                     /locus_tag="VYN69_001022"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEV1533311.1"
                     /translation="MKTKIIVIVGPTAVGKTALAIEVAKRFNGEVVSGDSQQVYRGLD
                     IGTAKASPEEQAAVPHHLIDVREITESYSAFDFVSEAKMTIEGIHNRGKLAIIAGGTG
                     LYIQSLLEGYHLGGETPHEEILAYRASLEPYSDEELAHLVEQAGLEIPQFNRRRAMRA
                     LEIAHFGQDLENQEILYEPLIICLDDERSQLYERINHRVDLMFEAGLLDEAKWLFDHS
                     PNVQAAKGIGYKELFPYFRGEQTFEEARESLKQATRRFAKRQLTWFRNRMQVTFYQIG
                     ESGVQDRILSQIEEFLDD"
     gene            10073..11311
                     /gene="hflX"
                     /locus_tag="VYN69_001023"
     CDS             10073..11311
                     /gene="hflX"
                     /locus_tag="VYN69_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEV1533312.1"
                     /translation="MIETEKKEERVLLIGVELQGMDSFDLSMEELASLAKTAGAVVVD
                     SYRQKREKYDSKTFVGSGKLEEIALMVDAEEITTVIVNNRLTPRQNVNLEEVLGVKVI
                     DRMQLILDIFAMRARSHEGKLQVHLAQFKYLLPRLVGQGIMLSRQAGGIGSRGPGESQ
                     LELNRRSVRNQITDIERQLKVVEKNRATVREKRLESSTFKIGLIGYTNAGKSTIMNIL
                     TSKTQYEADELFATLDATTKSIHLGGNLQVTLTDTVGFIQDLPTELVSSFKSTLEESK
                     HVDLLVHVIDASNPYHEEHEKTVLSIMKDLDMEDIPHLTLYNKADLVEDFTPTQTPYT
                     LISAKSEDSRENLQALLLDKIKEIFEAFTLRVPFSKSYKIHDLESVAILEERDYQEDG
                     EVITGYISEKNKWRLEEFYD"
     gene            11304..11927
                     /locus_tag="VYN69_001024"
     CDS             11304..11927
                     /locus_tag="VYN69_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HEV1533313.1"
                     /translation="MTDIKTLALKYGGYTSLDKVYLDQLLAGKTDQEQLTLITPPPSV
                     VNAYFAELYQKKSPQAATDYFAELSQELNLYNTEPSFTLENKPFIRLNLSGKSFGFCY
                     ESDGLGRIFFENEEKISDDLLFEIAQIFPHQLVFEESGKIYMKPVEDEEVISLESLTA
                     LTDLERLADGRKRLKGYSQEDLLQEAAAFSGKRYFRSENRTAMLYID"
     gene            11942..12871
                     /locus_tag="VYN69_001025"
     CDS             11942..12871
                     /locus_tag="VYN69_001025"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEV1533314.1"
                     /translation="MDIQFLGTGAGQPSKARNVSSLALKLLDEINEVWLFDCGEGTQN
                     RILETTIRPRKVSKIFITHLHGDHIFGLPGFLSSRAFQANEEQTDLEIYGPQGIKSFV
                     LTSLRVSGSRLPYRIHFHEFDQDSLGKILETDKFTVYAEELDHTIFCVGYRVMQKDLE
                     GTLDAEKLKAAGVPFGPLFGKIKNGQDLVLEDGTEIKAADYISAPRPGKIITILGDTR
                     KTDASVRLAVNADVLVHESTYGKGDEKIARNHGHSTNMQAAQVAVEAGAKRLLLNHIS
                     ARFLSKDISKLKKDAATIFENVHVVKDLEEVEI"
     gene            12892..13647
                     /locus_tag="VYN69_001026"
     CDS             12892..13647
                     /locus_tag="VYN69_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEV1533315.1"
                     /translation="MPTILITGASGGLAQEMVKLLPNDQLILLGRNKEKLAQLYGNYS
                     HAELIEIDITDDSALEALVTDLYLRYGKIDVLINNAGYGIFEGFDQIADKDIHQMFEV
                     NTFALMNLSRHLAARMKESSKGHIINIVSMAGLIATGKSSLYSATKFAAIGFSNALRL
                     ELMPYGVYVTTVNPGPIRTGFFDQADPDGTYLKSVDRFLLEADAVAKKIVKIIGKNKR
                     ELNLPILLNLAHKFYTLFPKLADKLAGETFNYK"
     gene            complement(13701..14609)
                     /locus_tag="VYN69_001027"
     CDS             complement(13701..14609)
                     /locus_tag="VYN69_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1533316.1"
                     /translation="MRIQQLHYIIKIVETGSMNEAAKQLFITQPSLSNAVRDLENEMG
                     IEIFIRNPKGITLTRDGMEFLSYARQVVEQTQLLEERYKNPVAHRELFSVSSQHYAFV
                     VNAFVSLLKKSDMEKYELFLRETRTWEIIDDVKNFRSEVGVLFLNSYNRDVLTKMLDD
                     NHLLAHHLFTAQPHIFVSKTNPLAKKDKVKLSDLENFPYLSYDQGTHNSFYFSEEILS
                     QEHHKKSIVVSDRATLFNLLIGLDGYTIATGILNSNLNGDNIVSIPLDIDDPIELVYI
                     QHEKTSLSKMGERFIDYLLEEVQFDS"
     gene            14673..14942
                     /locus_tag="VYN69_001028"
     CDS             14673..14942
                     /locus_tag="VYN69_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEV1533317.1"
                     /translation="MSLFLKKTQNFARMSLMKTFYDVQQFLKRFGIIVYMGKRLYDIE
                     LMKLELSRIYDAGLMDKLDYLEAEAVLRREHKVELDYIEKNGEKN"
     gene            14942..15322
                     /locus_tag="VYN69_001029"
     CDS             14942..15322
                     /locus_tag="VYN69_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEV1533318.1"
                     /translation="MVTWILWALILAMLAWMGFNYLRIRRAAKIVDNEEFEALIRTGQ
                     LIDLRDPAEFHRKHILGARNIPSSQLKTSLAALRKDKPVLLYENQRAQRVTNAALYLK
                     KQGFSEIYILSYGLDSWKGKVKTS"
     gene            15411..15638
                     /locus_tag="VYN69_001030"
     CDS             15411..15638
                     /locus_tag="VYN69_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000660075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533319.1"
                     /translation="MKEKKKNPLFVGILSIILGLLFPIVGLILGIIGLVLAISYQKES
                     QLDYKIEKILNILGIVISVVNWIVAIALIFR"
     gene            complement(15678..16403)
                     /locus_tag="VYN69_001031"
     CDS             complement(15678..16403)
                     /locus_tag="VYN69_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001235004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEV1533320.1"
                     /translation="MRLDNLLAQEKISRKAMKQALLRGEILVDGCPARSLAQNIDTGL
                     QELLFQDRIIQGYEHTYLMLHKPAGVVTANKDKELPTVMDLLPSDIQSDKLYAVGRLD
                     RDTTGLLLLTDNGPLGFQLLHPQYHVDKTYQVQVNGLLTPDHIQTFQKGIVFLDGTIC
                     KPARLEILSASPSFSQASITISEGKFHQIKKMFLSVGVKVTSLKRIQFGDFTLNPDLA
                     ESNYRPLNQKELQIIKNYLEMSR"
     gene            16565..18406
                     /gene="typA"
                     /locus_tag="VYN69_001032"
     CDS             16565..18406
                     /gene="typA"
                     /locus_tag="VYN69_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003078415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEV1533321.1"
                     /translation="MTKLREDIRNIAIIAHVDHGKTTLVDELLKQSETLDARTELAER
                     AMDSNDIEKERGITILAKNTAVAYNGTRINIMDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQDLVPIVVVNKIDKPSARPAEVVDEVLELFIELGADDDQ
                     LDFPVVYASAINGTSSLSDDPADQEATMAPIFDTIIDHIPAPVDNSDEPLQFQVSLLD
                     YNDFVGRIGIGRVFRGTVKVGDQVTLSKLDGTTKNFRVTKLFGFFGLERREIQEAKAG
                     DLIAVSGMEDIFVGETITPTDAVEALPILHIDEPTLQMTFLVNNSPFAGKEGKWVTSR
                     KVEERLQAELQTDVSLRVDPTDSPDKWTVSGRGELHLSILIETMRREGYELQVSRPEV
                     IVKEIDGVKCEPFERVQIDTPEEYQGSVIQSLSERKGEMLDMISTGNGQTRLVFLVPA
                     RGLIGYSTEFLSMTRGYGIMNHTFDQYLPLIPGEIGGRHRGALVSIDAGKATTYSIMS
                     IEERGTIFVNPGTEVYEGMIIGENSRENDLTVNITKAKQMTNVRSATKDQTAVIKTPR
                     ILTLEESLEFLNDDEYMEVTPESIRLRKQILNKAEREKANKKKKSAE"
     gene            18426..18680
                     /locus_tag="VYN69_001033"
     CDS             18426..18680
                     /locus_tag="VYN69_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3165 family protein"
                     /protein_id="HEV1533322.1"
                     /translation="MVYLVLGILLLLLYVFATPESIKGTVNIVAMVCILVALLILLVL
                     SFLKIFQLPTEIFLAIAMLILAYFSVRDITLMPVKKSKRR"
BASE COUNT         5864 a         3394 c         4093 g         5465 t
ORIGIN      
        1 aataaattaa ataaagaaca ccatctaaat actaaagaag ttctttctgt taccgcgatg
       61 aacaacaatg agtttatcac aaacctagat gaagctaata aaattattgt tcactatgcg
      121 gacggtacaa aagattactt taacttgtct tctagcagtg aaggtttaag taatgtaaaa
      181 gaatatacta taactgactt aggaattaaa tatacaccta atatcgttca aaaagataac
      241 actactcttg ttaatgatat aaaatctatt ttagaatcag tagagcttca gtctcaaacg
      301 atgtatcagc atctaaatcg attaggtgac tatagagtta atgcaatcaa agatttatat
      361 ttagaagaaa gcttcacaga tgttaaagaa aacttaacaa acctaatcac aaaattagtt
      421 caaaacgaag aacatcaact aaatgattct ccagctgctc gtcaaatgat tcgtgataaa
      481 gtcgagaaaa acaaagcagc tttattacta ggtttaactt acctaaatcg ttactatgga
      541 gttaaatttg gtgatgttaa tattaaagaa ttaatgctat tcaaaccaga tttctatggt
      601 gaaaaagtta gcgtattaga cagattaatt gaaatcggtt ctaaagagaa caacattaaa
      661 ggttcacgta cattcgacgc attcggtcaa gtattggcta aatatactaa atcaggtaat
      721 ttagatgcat ttttaaatta taatagacaa ttgttcacaa atatagacaa tatgaacgat
      781 tggtttattg atgctacaga agaccatgtc tacatcgcag aacgcgcttc tgaggtcgaa
      841 gaaattaaaa attctaaaca tcgtgcattc gataatttaa aacgaagtca ccttagaaat
      901 actatactcc cactactgaa tattgataaa gcacatcttt atttaatttc aaattataat
      961 gcaattgcct ttggtagtgc agagcgatta ggtaaaaaat cattagaaga tattaaagat
     1021 atcgttaaca aagctgcaga tggttataga aactattatg atttctggta tcgtctagcg
     1081 tctgataacg ttaaacaacg actactaaga gatgctgtta ttcctatttg ggaaggttat
     1141 aacgctcctg gtggatgggt tgaaaaatat ggccgctata ataccgacaa agtatatact
     1201 cctcttagag aattctttgg tcctatggat aagtattata attataatgg aacaggagct
     1261 tatgctgcta tatatcctaa ctctgatgat attagaactg atgtaaaata tgttcattta
     1321 gaaatggttg gtgaatacgg tatttcagtt tacacacatg aaacaacaca cgtcaacgac
     1381 cgtgcgattt acttaggtgg ctttggacac cgtgaaggta ctgatgctga agcatatgct
     1441 cagggtatgc tacaaactcc tgttactggt agtggatttg atgagtttgg ttctttaggt
     1501 attaatatgg tatttaaacg caaaaatgat gggaatcagt ggtatattac agatccaaaa
     1561 actctaaaaa cacgagaaga tattaataga tatatgaagg gttataatga cactttaact
     1621 cttcttgatg aaattgaggc tgaatctgtg atttctcaac aaaataaaga tttaaatagt
     1681 gcatggttca aaaaaataga tagagaatac cgtgataaca ataaattaaa tcaatgggat
     1741 aaaattcgaa atctaagtca agaagagaaa aatgaattaa atattcaatc tgttaatgat
     1801 ttagttgatc aacaattaat gactaatcgc aatccaggta atggtatcta taaacccgaa
     1861 gcaattagct ataacgatca atcaccttat gtaggtgtta gaatgatgac cggtatctac
     1921 ggaggtaata ctagtaaagg tgctcctgga gctgtttcat tcaaacataa tgcttttaga
     1981 ttatggggtt actacggata cgaaaatggg ttcttaggtt atgcttcaaa taaatataaa
     2041 caacaatcta aaacagatgg tgagtctgtt ctaagtgatg aatatattat caagaaaata
     2101 tctaacaata catttaatac tattgaagaa tttaaaaaag cttacttcaa agaagttaaa
     2161 gataaagcaa cgaaaggatt aacaacattc gaagtaaatg gttcttccgt ttcatcatac
     2221 gatgatttac tgacattgtt taaagaagct gttaaaaaag atgccgaaac tcttaaacaa
     2281 gaagcaaacg gtaataaaac agtatctatg aataatacag ttaaattaaa agaagctgtt
     2341 tataagaaac ttcttcaaca aacagatagc tttaaaactt caatctttaa ataattgaaa
     2401 tctctcgtct gctttgcgga tgagggtttt cttattttat gctatactta aggtatgcat
     2461 agaaaaacag tgattgattt taggactttg ggggagagat atacctttac caagcctatt
     2521 aaagagttga aaacgagaaa tgtagaagaa gtggcagatt tgctggcaca agtggaaagc
     2581 taccaagagc aaggttatta tgtggtgggg tatgtcagct acgaggctgc acctgctttt
     2641 gaggaaaaat tagcagttca caaggatccc ctactgggag agtacctgct ttattttact
     2701 gttcatgata gcgtagaaac ttcctctatt cctctgactt atgaggatat tgatttgccc
     2761 tcaaattggc aggaagtaac gtctgcagca gactatgaaa aggccattgc ccaaattcac
     2821 catcatttac ggcagggaga tacctatcag gtcaactaca ccgtccaact taagcaaaag
     2881 ttaaatgcca atccttttgc catctacaat cgtatggtgg tagagcagga ggcgggctac
     2941 aatgcctatg tggaacatga cgagatggca gtgatttcca tgagcccaga gctctttttt
     3001 gagcaaaatg atcgcgagtt gacaacacga ccaatgaagg ggacgactca gcgtggggta
     3061 actgaccaag aagatcttga acaggccagt tggttggaac aggatcccaa aaatcgctct
     3121 gaaaatatga tgattgtgga cctcttgcgc aatgatatga accgtatttc tgaagttggg
     3181 agcgagcacg tggagcgtct gtgtcaggta gagcagtatt caactgtttg gcagatgact
     3241 tcgaccatca agagtcagtt gcgagaggat gtggaccttg ttgaaatctt ccgctcactc
     3301 tttccttgtg gttccataac gggtgcaccg aaaattgcga caatggagat tatcaaggac
     3361 ttggagcctc aaccgcgtgg agtctactgt ggaacgattg gtctcttgct tccaaatgga
     3421 cgacggattt ttaatgtggc cattcgtacc attcaacttc acaaaggtca agccatttat
     3481 ggagttggcg gagggattac ttgggatagc acatgggaat ctgaataccg agaggttcat
     3541 caaaaggcag cagttcttta tcgtaaacaa gctcgcttcc aactgattac gacaggggaa
     3601 atcagccaga agaacctgtt gtttgaagat caacatctgg aaagactgag aaaagctagt
     3661 cgttattttg cctttccttt tgatgcagaa gacttgggac acaagattga ggaagagtgt
     3721 caggattgtg aagctaatcg agattaccgc ttgcgaatca gccttagcaa atctggagag
     3781 atagaagtca atcgtcaagt attaacccct ctcagtacaa gcttttgtca ggcccaagtc
     3841 tgccttcagg aagctgcttt gaatcaatcc tttacctact ttaaaaccac tcaccgaccg
     3901 catttgagcc taggagaaca agagaagatt taccacaata agtcaggaga actgcttgaa
     3961 acctctatag gaaatttggt tctgaaaatc gctggaaaac tctacacacc gcctatccga
     4021 cttggaatct tgccaggaat ttaccgtcag tatttgctag aaacaggaca ggtagaagag
     4081 aaagtcttga ccttggcaga tttagcccaa gcagaagcta tttacggctg taatgcagtg
     4141 agaggcttgt atgagttgca agtgatagga ttgtgagttt tagctattaa tagtttttaa
     4201 tatatattaa aatatattgg tgtttacttt tgatgagata tcatatttgt gttataatgt
     4261 aaccgtaaac aatagaaatt taaaggagat ttttgatgaa atcaaaaata gttttgggag
     4321 cttcgctagc gatcgcaaca cttagcttag tatctcttgt cgagattgaa ggactctcac
     4381 cgtttttaat agagaatgtt agcgcaaata ctcattctgc aaataaagtt attaatcata
     4441 aagtaagtat ttacttagaa aatgctgatg aaggtaaagg tctaactgta aacttttcaa
     4501 cagacagtgt tagtccaaat ttatttgatg agttcgaaaa aaagagtgga attacaataa
     4561 ctactatgtt agtgaatgct aagacaggtg aagttgtaga aaaaagatta acaccaagcg
     4621 tttttttacg ttccaatgat ttaaccagtg ggaccatatc ttcgtttatt tttagtgaat
     4681 atcctgatgg agaatataaa tatgttgtgt ctaaaggaga ctttatcgat cctaagactc
     4741 agtttaaaca tcagtataga ggagaatctc cagtttttag aatacgtaat cgaaaatatg
     4801 tggagttggg aacaactgat aaaaaattag atgaaagacg tgataatagc gtttataaag
     4861 atggggttgt tgaacacaag gtaaatttaa gtttaacaag ttatcaagga ggtaatggag
     4921 ttactgcaat tttttcaact gatagtgtta actctaactt acttaattct tttggtgaaa
     4981 aggctaaaaa agttttgatt aggtctaagt taattaatgt taagactggc gaggttattg
     5041 atgaaacgtt ttctccaaaa gtaagtttaa cctctaaaat tttgaaaagt ggttctacag
     5101 cagtatttta ttttatagat ttaactgatg gcgaatataa atatgttgcg tatgaaagcc
     5161 agcaatatac tgatcctcaa acaacattga ctcatcagta tagaggagaa tctcctattt
     5221 ttagtatcaa agatggaaaa ttttctggtt tagtatctgc ctcaaaacca gacgaaaacc
     5281 cgaagccaac gccaaaaccg gatgagaaac caaaaccttc agcaccacaa caggagaaac
     5341 caaaacctac agttcagtct ggttgggtag gttcatctta ttatcaaaat ggtaaaaaag
     5401 tcactagtaa gtggattttt gataaaaaat ataattcata cttctatcta gatgcatcag
     5461 gaaattatgt tcaaaacgct tgggtaggta actattatct caaatcaggc ggatacatgg
     5521 ctaaaagtga gtggatttat gataagaact atggttcata ctactatcta acatcagaag
     5581 gcagctacgc tcgtaacact tggtcaggca attattattt gaagtcaaat ggtaagatgg
     5641 ctaaaagtga gtgggtatac gatagtaact acaaatcata ttactacttg acatcagaag
     5701 gaagttatgc tcgtaacact tgggtaggca attactatct taaatcaaac ggtaaaatgg
     5761 ctgttaatga acgtacacca gatggttatc gtgtagatgg ttcaggtaaa tgggttaaat
     5821 aagaaactaa aaaataggat aataggtaat atcagatgaa taaaaaaaat tattttcatg
     5881 tgctttagca ttttcaacga ttggaacatt gtcaacgatt gataaatcag gagttcttcc
     5941 tttttcagcg caaacggtgc aggctgacat acaaacgctt accccaatgg caaaatatta
     6001 tattattctt ccgaaggatg cagaaatata taaaacttgg agaggtactg tcaatattcc
     6061 cattattgat gctacaaaaa ctactccaga attatcttat ttcaaagaag atcatcgcaa
     6121 ctatatagct aatgagaata agtctggtgc caattacata gagtggaagg gaacagtaga
     6181 agagtttaaa gaggccatta agaagttgac tgataaaaaa tcaactactg caacaccaaa
     6241 aaaggatgaa aagccaacac caaaaccgga tgagaagcca aaaccaacac ctacagttca
     6301 gtctggttgg gtaggttcat cctattatca agatggtaaa aaagtcatta gtaagtggat
     6361 ttttgataaa aaatataatt catacttcta tctagatgca tcaggaaatt atgttcaaaa
     6421 cgcttgggta ggtaactatt atcttaaatc aggcggatac atggctaaag gtgaatgggt
     6481 ttatgatgcc acctatcaag cttggtatta tttgacatca gatggttctt atgcttacag
     6541 tacatggcaa ggaaattact atctaaaatc ggatggtaaa atggctgtca atgaatgggt
     6601 tgatggtgga cgttattatg ttggcgctga cggagtttgg aaggaaggtc aagcaagtac
     6661 agtttcttct agtaatgata gcaatagtga atattctgct gctttaggaa aggcaaaaag
     6721 ttataattcg ttattccaca tgtcaaaaaa acgtatgtat agacaattaa cttctgattt
     6781 tgataaattt tcaaatgatg cagctcaata tgccattgat catttagata attaacagat
     6841 actggtaggc gaaaaaattc cttataattc atttacgcaa acctttgcat aagtgaatgg
     6901 attttgatat actagtactg taagcatttt caattactac acaatgaaaa aggagaatat
     6961 gatgagtcaa aagattattg ggattgacct tggtggaact tctatcaaat ttgcaatctt
     7021 aacaacagca ggagaaatcc aaggaaaatg gtcaatcaag accaacattt tggatgaggg
     7081 aagtcatatc gttgatgata tgattgagtc tattcaacat cgtttggact tgcttggatt
     7141 ggcagcagcg gacttccaag gcattggaat gggatcacca ggtgtggttg accgtgacaa
     7201 agggactgtt atcggtgcct acaacttgaa ctggaaaacc cttcaaccaa tcaaacaaaa
     7261 gattgaaaaa gctttgggca ttccattttt catcgataat gatgccaacg tagcagctct
     7321 tggtgagcgc tggatgggtg ctggagataa ccaaccagac gttgtcttta tgacactcgg
     7381 tactggtgtt ggtggcggta tcgtcgcaga aggcaaattg cttcacggtg ttgctggtgc
     7441 agcaggtgag cttggtcaca tcactgttga ctttgaccag ccaatctcat gtacttgtgg
     7501 taaaaaaggc tgccttgaga cagttgcttc agcaacaggg attgtcaact tgactcgtcg
     7561 ctatgccgat gaatacgaag gcgatgcagc cttgaaacgc ttgattgata acggagaaga
     7621 agtaactgct aagactgtct ttgatctcgc aaaagaagga gacgaccttg ctttgattgt
     7681 ttaccgtaac ttctcacgtt acttgggaat cgcttgtgct aacattggtt caatcctaaa
     7741 cccatcaaca atcgtcatcg gtggtggtgt gtcagctgcg ggagaattcc ttctacaagg
     7801 tgttcaaaaa gtttacgatg aaaatagttt cccacaagta cgcacatcta ctaaattagc
     7861 tcttgcaact ctaggaaatg acgctggagt tatcggagca gcatcacttg tattgcaata
     7921 agataatgag agaggaaaat gctaacctag agttagtaat gttcctcttt tattttttat
     7981 gctatactag ttaggacaat gaatgaggtg agagtaaatg acaaaagcag atacgatttt
     8041 taaagagaat attgaacgca ttctaaagga aggtgttttc tcagagcagg ctcgtcctaa
     8101 gtacaaggat gggactgttg ctaattccaa gtatgtaact ggtgcctttt ccgagtacga
     8161 cttatctaaa ggagaatttc ctatcacaac cttgcgtccc attgcaatca aatccgccat
     8221 caaagaagtt ctctggattt accaagacca gtcaaatagc ttagaagtgc ttaatgacaa
     8281 gtacaatgtt cactactgga atgactggga agttggagac acgggaacca ttggtgagcg
     8341 ctatggtgcc gttgttaaga aacacgacat tatcaataag cttctcaaac agttggaaac
     8401 caatccttgg aaccgccgca atattatttc gctctgggat taccaagctt tcgaagaaac
     8461 agatgggctg ctcccgtgcg cctttcagac catgtttgat gtccggcgtg ttgatgggga
     8521 aatctatctg gatgcgacct tgatccagcg ctccaatgac atgctagtag cccaccacat
     8581 caacgctatg cagtatgtgg ctttgcagat gatgattgcc aaacattttg gctggaaggt
     8641 tgggaagttc ttctacttca tcaacaacct ccatatctat gataatcaat ttgaacaagc
     8701 tcaggaattg ctccgtcggg agccgtcaaa ctgccaacca cgcttggttt taaatgttcc
     8761 tgatgggact aatttctttg atatcaaagc agaagatttt gagttggtgg attatgaccc
     8821 tgttaagcca cagttgaagt ttgacctagc tatttaaaag aatagaaaaa agaagttgag
     8881 aataatccca acttcttttg tttcttaacg tgatacgcgg cgacgagctg cttttttacg
     8941 gttttcttcg atgaaagctg ctttttgctc ttctggttcg attactttct ttttaaatgc
     9001 gtatactgca cctgcaacgg cagcgacagt tcctgcgaca cctgttacaa gacctttagc
     9061 gaatccttta gccatgagtc ttcctccttt atattctcaa tcagccagcc tcctcaagag
     9121 gtcacatttt tctgactgac ctttttgtgt tataataata gtaacgaaaa aatgggaatt
     9181 tttcaaggaa aaaagatgaa aacaaaaata attgtgattg ttggaccgac tgctgttgga
     9241 aagacggctc tagctattga agttgcaaag cgttttaatg gcgaagtggt tagtggagat
     9301 agccagcaag tctatcgagg acttgatatt gggacggcca aggctagtcc agaagagcag
     9361 gcagctgttc ctcatcattt aatcgatgtt agagagataa ccgagtctta ctcggctttt
     9421 gattttgttt cagaagctaa gatgactatt gagggtattc acaaccgtgg caagctagcc
     9481 attatcgccg gtgggactgg actttatatc cagagcttgc tagaaggtta ccacctaggt
     9541 ggggagactc ctcatgagga gattttagct tatcgagcta gtttggagcc atattcagat
     9601 gaggaattag cccatttggt ggagcaagca ggccttgaga ttccccagtt taatcgtcgt
     9661 cgtgctatgc gtgccttaga aattgcccat tttggtcagg atttggaaaa tcaagagatc
     9721 ttgtatgaac cgctgattat ctgcttggat gatgaacgta gtcaacttta tgagcgtatc
     9781 aaccaccgag tggatttgat gtttgaggct gggcttttgg atgaggccaa gtggctgttt
     9841 gaccattccc ctaatgtaca ggctgctaaa ggaattggct ataaggaact ctttccttat
     9901 ttccgtggag agcagacctt tgaggaagct cgtgagagtc ttaaacaggc gacccgtcgt
     9961 tttgccaagc gtcagctgac ctggttccgt aatcgcatgc aggtcacctt ttatcagatt
    10021 ggagaatctg gtgtacaaga ccgcatttta agccagatag aggagttttt agatgattga
    10081 aacggagaaa aaagaggagc gagtcctgct gattggtgtg gaattgcagg gtatggacag
    10141 ttttgacctc tccatggaag aattggctag tttagcgaaa acggcagggg cagtcgttgt
    10201 agatagctac agacaaaaac gtgaaaaata tgattccaag accttcgtcg gctctggtaa
    10261 gttggaagag attgcgctta tggtggatgc agaagaaatc actactgtca tcgtcaacaa
    10321 tcgtctgacc ccaaggcaga atgtcaatct agaggaagtt ctcggtgtta aggtcattga
    10381 ccgtatgcag ttgattttgg atatctttgc catgcgggct cgaagccatg aagggaagct
    10441 ccaagtccac ctagcccaat tcaaatacct cttgcctcgc ttggttggtc aggggattat
    10501 gctcagccgt caggcagggg gaattggttc ccgtggtcct ggtgaaagcc aactggagct
    10561 gaaccgtcgt agcgttcgca atcaaatcac ggatatcgag cgccagctta aggtggttga
    10621 gaaaaatcgt gcgactgtca gagaaaaacg tttggagtct agcactttta agattggttt
    10681 gattggttat actaatgctg ggaaatcaac tatcatgaac atcttgacca gtaagaccca
    10741 gtatgaagca gatgagctct ttgcgactct ggatgcgaca accaagagta ttcatctggg
    10801 aggcaatctc caagtaactt tgacagatac cgttggcttt atccaagatt tgccgacaga
    10861 gttggtgtcc agtttcaagt caaccttgga agaaagcaag catgtggacc ttctggttca
    10921 tgttatcgat gctagcaatc cttaccacga ggagcatgaa aaaacggttc tctccatcat
    10981 gaaagacctg gacatggaag atattcctca cttgacgctt tataataaag cggatttggt
    11041 ggaggatttc acgcctaccc aaacgccata taccctcatt tctgccaagt ctgaggacag
    11101 tcgtgaaaac ttgcaagcat tattgctaga taagattaag gaaatttttg aagcatttac
    11161 cctgcgagtg cctttttcaa agtcctacaa gattcatgat ttagagagtg ttgcaattct
    11221 ggaagaacgt gattatcagg aagacggcga agtgattaca ggctacattt ccgagaaaaa
    11281 taaatggagg ttagaagaat tttatgactg atattaaaac tttggctcta aagtatggag
    11341 gttatacaag tctggacaag gtctatctgg atcagcttct agctggtaaa acagatcagg
    11401 agcagttaac acttattaca cctccgccaa gtgttgttaa tgcctacttt gcagaactct
    11461 atcagaaaaa gagtcctcag gctgcgacgg attattttgc agaactcagt caggaactga
    11521 atctttacaa tactgagcca agtttcaccc tagaaaataa gccttttatt cggcttaatc
    11581 tgtctggtaa atcctttggt ttttgttatg agagtgacgg ccttggtcgg attttctttg
    11641 aaaatgagga gaaaatctcg gatgacttgc tctttgaaat tgcgcaaatt ttcccccatc
    11701 aattagtctt tgaagagtct ggtaagattt acatgaagcc tgtcgaggac gaggaagtta
    11761 tcagcctaga aagtctcaca gctttgactg atttggaaag attggctgat ggtcgaaaac
    11821 gtctcaaagg ctacagccaa gaggatttat tgcaagaagc tgctgctttt tctggcaaac
    11881 gctatttccg atcggaaaac cgcacagcca tgttatatat tgattaatta gaaagtatcg
    11941 aatggatatt caatttttag gaacgggggc tggtcagccc tctaaagccc gcaacgtttc
    12001 aagtctcgcc ctgaaacttt tggatgagat taacgaagtt tggctctttg actgtggaga
    12061 aggtacgcaa aatcgcattc tggaaaccac aattcgacca cgtaaggtca gcaaaatctt
    12121 tattacccat ctgcatggag accacatttt tggtttgcca ggtttccttt ctagccgtgc
    12181 ctttcaggcc aatgaagagc agacagattt ggaaatctac ggacctcaag gaatcaagtc
    12241 atttgtctta accagccttc gtgtgtcagg ttctcgtctg ccctaccgca ttcatttcca
    12301 tgagtttgac caagattctc taggtaaaat tcttgaaacc gataaattca ctgtgtatgc
    12361 agaggagctg gaccacacta ttttctgtgt tggctatcgt gtcatgcaaa aggatctaga
    12421 agggacgctg gatgctgaaa aactcaaggc tgctggtgtt ccgttcggcc cgctttttgg
    12481 taaaatcaaa aatggccagg atcttgtttt ggaagacgga actgaaatca aggcagcaga
    12541 ctatatctca gcgccacgtc caggtaagat tatcactatt ttaggagaca ctcgaaaaac
    12601 ggatgccagt gtgcgtctgg ctgtcaatgc agatgtccta gttcatgagt ccacttatgg
    12661 caagggtgat gaaaaaattg ctcgtaacca tggtcactca actaatatgc aagctgcaca
    12721 agtagcggta gaagcaggtg ccaaacgcct cctactcaac catatcagtg cccgtttcct
    12781 ctcaaaagat attagcaaac tcaagaaaga cgctgccaca atttttgaaa atgtccatgt
    12841 ggtcaaagac ttggaagaag tggaaatcta gcagtcacag aaaggataag tatgcctact
    12901 attctcatta ccggagctag cggtggtcta gctcaagaaa tggtcaaact cctgcccaat
    12961 gaccaactca tcttgcttgg tagaaataag gaaaaattag cccaactcta cggaaattat
    13021 tcccatgcag aattgattga aattgatatt accgacgatt cagccctaga agctctggta
    13081 actgatcttt atctccgcta tggcaagatt gatgtcttga ttaacaacgc tggttacggg
    13141 atttttgagg gatttgacca gattgctgat aaagatattc accagatgtt tgaggtcaat
    13201 acctttgccc tgatgaatct gtctcgtcac cttgcggctc gtatgaagga aagcagcaaa
    13261 gggcatatca tcaacatcgt cagcatggca ggtctaatag ctactggcaa gtctagtctt
    13321 tactcagcga ccaagtttgc ggctattggt ttttcaaatg ctctgcgact cgaacttatg
    13381 ccctatggag tctatgtgac aacagtcaat ccaggtccaa tccgaacagg attttttgac
    13441 caagctgacc cagatggaac ttatcttaaa tcggttgacc gcttcctctt agaggcagat
    13501 gcagtggcta aaaagattgt caagattata ggcaaaaata aacgagaact caatctcccg
    13561 attttgttga acctagccca taagttttat actctctttc ccaagctagc tgataagttg
    13621 gcaggggaaa cttttaatta taagtaaaaa gaaccaatgt gcaggttgtt gctagcctac
    13681 atattggttc ttatcatttc tcaactatca aactgaactt cttctagtag atagtcgata
    13741 aagcgttcgc ccatcttaga taggctggtt ttctcatgct ggatatagac cagctcaatc
    13801 gggtcatcaa tatccagtgg gatagaaacg atattgtctc cgtttaggtt gctgttcaaa
    13861 atccctgtcg caatggtata accatccaaa ccaatcaaga gattaaagag ggtggcacgg
    13921 tcactgacca caatggattt cttgtggtgt tcttgagaaa gaatctcttc tgaaaagtag
    13981 aaggagttgt gcgtcccttg gtcatagctg aggtaaggga aattctccaa atcagacagt
    14041 ttcaccttgt ctttctttgc cagagggttg gtcttgctga caaagatatg cggttgcgct
    14101 gtgaagagat ggtgggctag caggtgattg tcatccagca tcttggttaa aacatcacgg
    14161 ttgtaactgt ttaagaagag gaccccgacc tcactgcgga agttcttgac gtcgtcgata
    14221 atctcccaag tccgagtttc acgaaggaag agttcgtatt tctccatatc gcttttcttg
    14281 agcaaagaga caaaggcatt gaccacaaag gcatagtgtt gagacgaaac gctaaagagt
    14341 tcgcggtggg cgacaggatt tttatagcgt tcctccagaa gctgggtctg ctcgacaacc
    14401 tgacgggcat aagagagaaa ctccatccca tcacgggtca aggtgattcc cttgggattg
    14461 cggataaaga tctcaatgcc catttcattt tccaaatctc gcactgcatt ggagagactt
    14521 ggctgagtga taaagagttg cttggctgcc tcattcatgg agccagtttc gacgattttg
    14581 ataatataat gtaattgttg aattctcatg tttttattgt accatacttg ccttagaatc
    14641 gctaaagaag ataggaaaat aaggagcatt gtgtgagtct cttcttgaaa aaaacacaaa
    14701 attttgctag aatgagtctt atgaaaacat tctatgatgt gcagcaattc ctcaaacgat
    14761 ttggtattat tgtttacatg ggaaaacgct tatatgatat tgaactgatg aagttggaac
    14821 tatctcggat ttacgatgca gggttgatgg ataaattaga ctatctagag gcagaagcag
    14881 ttcttcgaag agagcacaag gtagaattgg attatattga gaaaaatgga gaaaagaact
    14941 aatggttact tggattttgt gggcacttat actagcaatg ttggcgtgga tgggctttaa
    15001 ctatcttcgt attcgccgtg cggctaaaat tgtggacaat gaggagtttg aagccttgat
    15061 tcgtacgggt caattgattg atttgcgcga cccagcagaa ttccacagaa aacatatcct
    15121 tggtgcacgc aatattcctt caagtcagtt gaaaactagt cttgcagccc ttcgtaaaga
    15181 taaacctgtc cttctctacg aaaaccaacg tgcgcaacga gttacaaatg cagctcttta
    15241 cttgaaaaaa caaggttttt ctgagattta tatcctttct tatggcttgg attcttggaa
    15301 agggaaagtg aagactagct aaccttattt ccaaattagt cgaatgatag gatgaagcta
    15361 ttgttttata gtataattaa tttgtataaa atttaaggag ttcttagaac ataaaagaaa
    15421 agaagaaaaa tccacttttt gtgggtatct tgtcgattat tcttggtttg ttatttccaa
    15481 ttgtaggcct gattttgggt atcataggat tggtcttggc tatttcatac caaaaagagt
    15541 cacaattaga ctataaaata gaaaagattc ttaatatctt aggaattgtg atttctgtag
    15601 ttaactggat tgtagcaatc gccttaattt ttcggtaaat aaaagaaaaa agctttagtt
    15661 ttaaagcttt ttttgtttta tcgactcatc tctaaatagt ttttaatgat ttgtaactct
    15721 ttttggttca aagggcggta gttactttct gctaaatctg ggttcaatgt gaagtcccca
    15781 aattggattc tcttgaggct agtcacctta acaccaaccg agaggaacat tttcttgatt
    15841 tgatgaaatt ttccttctga aatggtgata gaggcttgac tgaaggaagg acttgctgat
    15901 agaatctcta gtcttgcagg tttacagata gtgccatcta aaaagacaat tcctttttga
    15961 aaggtttgga tatggtcagg tgttagaagt ccattaacct ggacttggta agtcttatcg
    16021 acatgatatt ggggatggag gagttggaag cccaagggac cgttatcggt caagagaagg
    16081 agtcctgttg tatctcggtc cagtcggccg acggcataga gcttgtcaga ctggatgtca
    16141 gatggaagca ggtccatgac ggtcggaagt tccttgtctt tgttggctgt aacgacacca
    16201 gcaggtttat gaagcataag ataggtgtgt tcatagcctt gaatgattcg atcctgaaaa
    16261 aggagttctt gtagtcctgt atcgatattt tgagctaggg agcgggctgg gcaaccatcg
    16321 actagaattt cccctctgag gagtgcttgc ttcatggcct ttcggctgat tttttcttgg
    16381 gctaataaat tatctaaacg catactgtgc ctatcttatc ataagtcgct cgctttgaaa
    16441 aggcttgacg tgaaaagaaa agcagagtga aaacatttac acttgcttga gttatgttat
    16501 ttacttgaaa ttatggtata atcgttcagt tagaaaataa attttgaata ttatagagga
    16561 aatcatgaca aaattaagag aagatatccg taacattgcg attatcgccc acgttgacca
    16621 cggtaaaaca accctggttg acgaattatt gaaacaatca gaaacgcttg atgcacgtac
    16681 tgaattggca gagcgtgcta tggactcaaa cgatatcgaa aaagagcgtg ggattaccat
    16741 ccttgctaaa aatactgccg ttgcttacaa cggaactcgt atcaacatta tggacacacc
    16801 aggacacgcg gactttggtg gagaagttga gcgtatcatg aaaatggttg acggtgttgt
    16861 cttggtcgta gatgcctatg aaggaaccat gccacaaact cgtttcgtat tgaaaaaagc
    16921 cttggaacaa gaccttgtcc caatcgtggt tgttaacaaa atcgataagc catcagctcg
    16981 tccagcagaa gtagtggatg aagtcttgga acttttcatc gagcttggtg cagatgacga
    17041 ccagcttgat ttcccagtgg tttatgcttc agcgatcaac ggaacttctt cattgtcaga
    17101 tgatccagct gaccaagaag cgactatggc accaatcttt gacacgatta tcgaccatat
    17161 cccagctcca gtagataact cagatgagcc tttgcagttc caagtgtcgc ttttggacta
    17221 caatgacttc gttggacgta tcggtatcgg tcgtgtcttc cgtggtacag ttaaggttgg
    17281 ggaccaagtt accctttcta aacttgacgg tacaactaaa aacttccgtg ttacaaaact
    17341 cttcggtttc tttggtttgg aacgtcgtga aatccaagaa gccaaagcgg gtgacttgat
    17401 tgccgtttca ggtatggaag acatctttgt cggtgaaacc atcactccga cagatgcagt
    17461 agaagctctt ccaatcctac acatcgatga gccaactctt caaatgactt tcttggtcaa
    17521 caactcacca tttgctggta aagaaggtaa atgggtaact tctcgtaagg tggaagaacg
    17581 cttgcaggca gaattgcaaa cagacgtttc ccttcgtgtt gacccaactg attcaccaga
    17641 taaatggact gtttcaggac gtggagaatt gcacttgtca atccttatcg aaacaatgcg
    17701 tcgtgagggc tatgaacttc aagtatctcg tccagaagtt atcgtaaaag aaatcgacgg
    17761 tgttaaatgt gaaccatttg aacgtgtaca aatcgacact ccagaagaat accaagggtc
    17821 tgttatccaa agcctttctg aacgtaaagg tgaaatgttg gatatgattt caactggtaa
    17881 tggtcaaact cgtttggtct tccttgttcc agcgcgtggt ttgattggat actcaactga
    17941 gttcttgtca atgactcgtg gttacggtat catgaaccat accttcgacc agtacttgcc
    18001 attgattcca ggggaaattg gtggacgtca ccgtggtgcc cttgtttcta tcgatgctgg
    18061 taaggctaca acttactcaa tcatgtctat cgaagaacgt ggtacgatct ttgtcaaccc
    18121 aggtactgag gtttatgaag gaatgatcat cggcgaaaac tctcgtgaaa atgacttgac
    18181 agttaacatc acgaaggcaa aacaaatgac caacgttcgt tcagctacta aggaccaaac
    18241 agctgttatc aagacgcctc gtatcttgac acttgaagag tctcttgagt tcttgaacga
    18301 cgatgagtac atggaagtaa cgcctgagtc tatccgtttg cgtaaacaaa tccttaacaa
    18361 agcagagcgt gagaaagcta acaagaagaa aaaatcagct gaataagagc tagaaagaga
    18421 gaaagatggt ctatttagtc ctaggaattt tactgctcct actctatgta tttgcgacac
    18481 cagaaagcat taaagggaca gtcaatatcg tcgctatggt atgtatttta gtggcactct
    18541 tgattttatt ggttctatct tttctgaaaa tttttcaatt accaacagaa atattcctag
    18601 caatagccat gttgatccta gcttacttta gtgttagaga cattacactc atgccagtca
    18661 aaaaaagtaa aagaagataa aaaagagagt tttggctctc ttttttgcta ggaattttac
    18721 aatagtttga attgttttgt actataacat catctctttt gcttggatag tatgaatggg
    18781 aaaaacttga ataaaattcg ttaatggcat ctattt
//
LOCUS       DAVLVP010000031        18387 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.031, whole genome shotgun sequence.
ACCESSION   DAVLVP010000031 DAVLVP010000000
VERSION     DAVLVP010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 18387)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18387)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18387
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.031"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            832..2091
                     /locus_tag="VYN69_001034"
     CDS             832..2091
                     /locus_tag="VYN69_001034"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEV1533323.1"
                     /translation="MRKIVINGGLPLQGEITISGAKNSVVALIPAIILADDVVTLDCV
                     PDISDVASLVEIMELMGATVKRYDDVLEIDPRGVQNIPMPYGKINSLRASYYFYGSLL
                     GRFGEATVGLPGGCDLGPRPIDLHLKAFEAMGATASYEGDNMKLSAKDTGLHGASIYM
                     DTVSVGATINTMIAAVKANGRTIIENAAREPEIIDVATLLNNMGAHIRGAGTNIIIID
                     GVERLHGTRHQVIPDRIEAGTYISLAAAVGKGIRINNVLYEHLEGFIAKLEEMGVRMT
                     VSEDSIFVEEQSNLKAINIKTAPYPGFATDLQQPLTPLLLRANGRGTIVDTIYEKRVN
                     HVFELAKMDADISTTNGHILYTGGRDLRGASVKATDLRAGAALVIAGLMAEGKTEITN
                     IEFILRGYSDIIEKLRNLGADIRLVED"
     gene            2107..2658
                     /locus_tag="VYN69_001035"
     CDS             2107..2658
                     /locus_tag="VYN69_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1533324.1"
                     /translation="MNIWTKLAMFSFFETDRLYLRPFFFSDSQDFREIASNPENLQFI
                     FPTQASLEESQYALANYFMKSPLGVWAICDQKNQQMIGSIKFEKLDEIKKEAELGYFL
                     RKDAWSQGFMTEVVRKICQLSFEEFGLKQLSIITHLENEASQRVALKSGFSLFRQFKG
                     SDRYTRKMRDYLEFRYVKGEFNE"
     gene            2651..3928
                     /locus_tag="VYN69_001036"
     CDS             2651..3928
                     /locus_tag="VYN69_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEV1533325.1"
                     /translation="MSKHQEILSYLEELPVGKRVSVRSISNHLGVSDGTAYRAIKEAE
                     NRGIVETRPRSGTIRVKSQKVAIERLTFAEIAEVTSSEVLAGQEGLEREFSKFSIGAM
                     TEQNILSYLHDGGLLIVGDRTRIQLLALENENAVLVTGGFQVHDDVLKLANQKGIPVL
                     RSKHDTFTVATMINKALSNVQIKTDILTVEKLYRPSHEYGFLRETDTVKDYLDLVRKN
                     RSSRFPVINQHQVVVGVVTMRDAGDKSPSTTIDKVMSRSLFLVGLSTNIANVSQRMIA
                     EDFEMVPVVRSNQTLLGVVTRRDVMEKMSRSQVSALPTFSEQIGQKLSYHHDEVVITV
                     EPFMLEKNGVLANGVLAEILTHMTQDLVVNSGRNLIIEQMLIYFLQAVQIDDILRIQA
                     RIIHHTRRSAIIDYDIYHGHQIVSKANVTVKIN"
     gene            3944..4804
                     /locus_tag="VYN69_001037"
     CDS             3944..4804
                     /locus_tag="VYN69_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl aminopeptidase"
                     /protein_id="HEV1533326.1"
                     /translation="MITLKSAREIEAMDKAGDFLASIHIGLRDLIKPGVDMWEVEEYV
                     RRRCKEENFLPLQIGVDGAMMDYPYATCCSLNDEVAHAFPRHYILKDGDLLKVDMVLG
                     GPIAKSDLNVSKLNFNNVEQMKKYTQSYSGGLADSCWAYAVGTPSEEVKNLMDVTKEA
                     MYKGIEQAVVGNRIGDIGAAIQEYAESRGYGVVRDLVGHGVGPTMHEEPMVPNYGIAG
                     RGLRLREGMVLTIEPMINTGDWEIDTDMKTGWAHKTIDGGLSCQYEHQFVITKDGPVI
                     LTSQGEEGTY"
     gene            5185..5987
                     /locus_tag="VYN69_001038"
                     /pseudo
     CDS             5185..5987
                     /locus_tag="VYN69_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="programmed frameshift; internal stop; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            6179..8470
                     /gene="pcrA"
                     /locus_tag="VYN69_001039"
     CDS             6179..8470
                     /gene="pcrA"
                     /locus_tag="VYN69_001039"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000992850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEV1533327.1"
                     /translation="MNALLNGMNDRQAEAVQTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     IDEKLVNPWNILAITFTNKAAREMKERAYSLNPATQDCLIATFHSMCVRILRRDADHI
                     GYNRNFTIVDPGEQRTLMKRILKQLNLDPKKWNERTILGTISNAKNDLIDDVAYAAQA
                     GDMYTQIVAQCYTTYQKELRQSESVDFDDLIMLTLRLFDQNPDVLTYYQQKFQYIHVD
                     EYQDTNHAQYQLVKLLASRFKNICVVGDADQSIYGWRGADMQNILDFEKDYPKAKVVL
                     LEENYRSTKTILQAANEVIKNNKNRRPKNLWTQNADGEQIVYYRADDELDEAVFVART
                     IDELSRSQNFLHKDFAVLYRTNAQSRTIEEALLKSNIPYTMVGGTKFYSRKEIRDIIA
                     YLNLIANLSDNISFERIINEPKRGIGLGTVEKIRDFANLQNMSMLDASANIMLSGIKG
                     KAAQSIWDFANMMLDLREQLDHLSITELVESVLEKTGYVDILNAQATLESKARVENIE
                     EFLSVTKNFDDTTDVTEEETGLDKLSRFLNDLALIADTDSGSQETSEVTLMTLHAAKG
                     LEFPVVFLIGMEENVFPLSRATEDPDELEEERRLAYVGITRAEKILYLTNANSRLLFG
                     RTNYNRPTRFINEISSDLLEYQGLARPANTSFKASYSSGSISFGQGMSLAQALQDRKR
                     GAAPKSIQSSGLPFGQFTAGAKPASSEANWSIGDIALHKKWGEGTVLEVSGSGARQEL
                     KINFPEVGLKKLLASVAPIEKKI"
     gene            8513..9193
                     /gene="radC"
                     /locus_tag="VYN69_001040"
     CDS             8513..9193
                     /gene="radC"
                     /locus_tag="VYN69_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEV1533328.1"
                     /translation="MYSISFQEDSLLPRERLAKEGVEALSNQELLAILLRTGTRQASV
                     FEIAQKVLNNLSSLTDLKKMTLQELQSLSGIGRVKAIELQAMIELGHRIHKHETLEME
                     SILSSQKLAKKMQQELGDKKQEHLVALYLNTQNQIIHQQTIFIGSVTRSIAEPREILH
                     YAIKHMATSLILVHNHPSGAVAPSQNDDHVTKLVKEACELMGIVLLDHLIVSHSNYFS
                     YREKTDLI"
     gene            complement(9190..9879)
                     /locus_tag="VYN69_001041"
     CDS             complement(9190..9879)
                     /locus_tag="VYN69_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEV1533329.1"
                     /translation="MKKPVIGITGNEKTHPDDDIMMSYAAKGFVEGVKDAGGIPIILP
                     IGDQEMACHYISLIDKLILTGGQNVDPKFYGEPKTIDSDDYHLQRDIFELALIKEAIK
                     QKKPIFSVCRGTQLFNVAMGGTLYQDIEDHWQDSSVEYTTQRLVTEPDTVLQEIYGEI
                     SHINSFHHQSIKDLAPNLKVVAHDPKDGIIEAVMSTDDVAFLGVQWHPELLFENRPKD
                     KKLFDYVVNEL"
     gene            complement(9897..10538)
                     /locus_tag="VYN69_001042"
     CDS             complement(9897..10538)
                     /locus_tag="VYN69_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEV1533330.1"
                     /translation="MKDKQFAIPKATAKRLSLYYRIFKRFHAEKIERANSKQIAEAIG
                     IDSATVRRDFSYFGELGRRGFGYDVKKLMTFFADLLNDNSITNVMLVGIGNMGHALLH
                     YRFHERNKMKIIMAFDLDDHPEVGTQTPDGIPIYGISQIKDKIKDADVKTAILTVPSV
                     KSQEVANLLVDAGVKGILSFSPVHLHLPKDVVVQYVDLTSELQTLLYFMRKED"
     gene            complement(10621..10836)
                     /locus_tag="VYN69_001043"
     CDS             complement(10621..10836)
                     /locus_tag="VYN69_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000462494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4649 family protein"
                     /protein_id="HEV1533331.1"
                     /translation="MFELTYKDCYHVERTLKYEDHEALMLTLSGCVTLPDTLYVTSLT
                     FRGQKVYQGLVGDLYRFLSHADFLHQN"
     gene            complement(10844..11191)
                     /locus_tag="VYN69_001044"
     CDS             complement(10844..11191)
                     /locus_tag="VYN69_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEV1533332.1"
                     /translation="MAFEKIIQLKNCRYDYTLSPSVKKFTLKDNTFFETKVGNYELTR
                     LLEKVPNSGEGFQLKIIINKELTGAKINITDKFGLRLVDIFKSEDHHIHQEKFYFLMD
                     SLVERGVFTKSER"
     gene            complement(11196..12311)
                     /locus_tag="VYN69_001045"
     CDS             complement(11196..12311)
                     /locus_tag="VYN69_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1533333.1"
                     /translation="MIYLDNAATTPMSAVAISAMTKVMQETHGNPSSIHGHGRQAGKL
                     LREARQELAQLLRTKPQHIFFTSGGTEGNNTTIIGYCLRHQEQGKHIITTAIEHHAVL
                     ETIDYLVQHFGFEATIIQPENQEITAQQIQKALRDDTILVSTMFANNETGNLLPIAEI
                     GQILKQHPAAYHVDAVQAIGKIPIHSEELGIDFLTASAHKFHGPKGIGFLYASSMDFD
                     SYLHGGDQEQKKRAGTENLPAIVGMVAALKEDLEKQEEHFQHVQNLETAFLAELEGIQ
                     YYLNRGKHHLPYVLNIGFPGQKNDLLLLRLDLAGISISTGSACTAGVVQSSHVLEAMY
                     GANSERLKESLRISLSPQNTVEDLQTLAKTLKEIIGG"
     gene            complement(12321..13280)
                     /locus_tag="VYN69_001046"
     CDS             complement(12321..13280)
                     /locus_tag="VYN69_001046"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1533334.1"
                     /translation="MSDRKNMKLFALNSNQEIAQKIAQAVGVPLGKLSSRQFSDGEIQ
                     VNIEESVRGYDVYIIQSTSFPVNNHLMELLIMVDACVRASAHSINVVLPYFGYARQDR
                     IACPREPLTAKLVANMLVKAGVDRILTLDLHAVQVQGFFDIPVDNLFTVPLFAKHYCD
                     KGLLGSDVVVVSPKNSGVKRARSLAEYLDAPIAIIDYPQDDATRNEGYIIGDVEGKKA
                     ILIDDILNTGRTFSEASKIVEREGATEIYAVSSHGLFVEGAAELLDNTNIKEILVTDS
                     VATKEKTPKNVCYITASELIGDAIVRIHERKPVSPLFAYNKKK"
     gene            complement(13393..13962)
                     /locus_tag="VYN69_001047"
     CDS             complement(13393..13962)
                     /locus_tag="VYN69_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEV1533335.1"
                     /translation="MKHLEIELKTLLKKDEYNRLKDQFTGVTPVLQTNYYIDTPDFEL
                     REKKVAMRIRTFEDWAELTLKVPQSVGNMEYNQKLQLKDAENYLSKEELPQGLVLDEL
                     AKHGIQSKNWQVLGCLTTLRYEMKTAIGLMALDQSRYFDITDYELELEVENHEQGKQD
                     FRQFLEKNQISYQKAPSKLVRFVKSMKNS"
     gene            14090..14761
                     /locus_tag="VYN69_001048"
     CDS             14090..14761
                     /locus_tag="VYN69_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000171686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEV1533336.1"
                     /translation="MTLEWEEFLDPYIQAVGELKIKLRGIRKQYRKQNKHSPIEFVTG
                     RVKPIESIKEKMARRGITYATLEHDLQDIAGLRVMVQFVDDVKEVVDILHKRQDMRII
                     QERDYITHRKASGYRSYHVVVEYTVDTINGAKTILAEIQIRTLAMNFWATIEHSLNYK
                     YQGDFPDEIKKRLEITARIAHQLDEEMGEIRDDIQEAQALFDPLSRKLNDGVGNSDDT
                     DEEYR"
     gene            14745..15563
                     /locus_tag="VYN69_001049"
     CDS             14745..15563
                     /locus_tag="VYN69_001049"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEV1533337.1"
                     /translation="MKNTGKRIDLIANRKPQSQRVLYELRDRLKRNQFILNDTNPDIV
                     ISIGGDGMLLSAFHKYENQLDKVRFIGLHTGHLGFYTDYRDFELDKLVTNLQLDTGAR
                     VSYPVLNVKVFLENGEVKIFRALNEASIRRSDRTMVADIVINGVPFERFRGDGLTVST
                     PTGSTAYNKSLGGAVLHPTIEALQLTEIASLNNRVYRTLGSSIIVPKKDKIELIPTRN
                     DYHTISVDNSVYSFRNIERIEYQIDHHKIHFVATPSHTSFWNRVKDAFIGEVDE"
     gene            15560..16456
                     /locus_tag="VYN69_001050"
     CDS             15560..16456
                     /locus_tag="VYN69_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1533338.1"
                     /translation="MRFEFIADEHVKVKTFLKKHEVSKGLLAKIKFRGGAILVNNQPQ
                     NATYLLDVGDYVIIDIPAEKGFETLEAIELPLDILYEDDHFLVLNKPYGVASIPSVNH
                     SNTIANFIKGYYVKQNYENQQVHIVTRLDRDTSGLMLFAKHGYAHARLDKQLQKKSIE
                     KRYFALVKGDGHLEPEGEIIAPIARDEDSIITRRVAKGGKYAHTSYKIVASYGNIHLV
                     YIHLHTGRTHQIRVHFSHIGFPLLGDDLYGGSLEDGIQRQALHCHYLSFYHPFLEQDL
                     QLESPLPDDFSNLITQLSTNTL"
     gene            16500..17474
                     /gene="pta"
                     /locus_tag="VYN69_001051"
     CDS             16500..17474
                     /gene="pta"
                     /locus_tag="VYN69_001051"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEV1533339.1"
                     /translation="MEVFESLKANLVGKNARIVLPEGEEPRILQATKRLVKETEVIPV
                     LLGNPEKIKIYLEIEGIMDGYEVIDPQHYPQFEEMVSALVERRKGKMTEEDVRKVLVE
                     DVNYFGVMLVYLGLVDGMVSGAIHSTASTVRPALQIIKTRPNVTRTSGAFLMVRGTER
                     YLFGDCAININPDAEALAEIAINSAITAKMFGIEPKIAMLSYSTKGSGFGESVDKVVE
                     ATKIAHDLRPDLEIDGELQFDAAFVPETAALKAPGSTVAGQANVFIFPGIEAGNIGYK
                     MAERLGGFAAVGPVLQGLNKPVNDLSRGCNADDVYKLTLITAAQAVHQ"
     gene            complement(17815..18114)
                     /locus_tag="VYN69_001052"
     CDS             complement(17815..18114)
                     /locus_tag="VYN69_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEV1533340.1"
                     /translation="MMNMQNMMRQAQKLQKQMEQSQAELAAMQFVGKSAQDLVQATLT
                     GDKKVVSIDFNPAVVDPEDLETLSDMTVQAINSALEQIDETTKKKLGAFAGKLPF"
BASE COUNT         5427 a         3352 c         4016 g         5592 t
ORIGIN      
        1 actatcgtga ggtagtggat taaaacggtc tggggataga ccgttttaag tctgacgctg
       61 gaaataagaa tcgtcagagg aagggatagc gaaatcgtgg ctttacgaac aggaacgtga
      121 tagtaaggcg tatatagcgg ataagggggt ctcaaactct aaagtccaaa aaggtagtcg
      181 taacctatat gcgtaaatca cgagagtaat tgaattcgta ctaagatttt ctattttcac
      241 tgtaaccttt taacgtcctt atatcttgta tacacgagga aagatgtacg acttatcccg
      301 tgaggtctat cactataaag agaaaacgac agatagaagt gatcctgagt cacggttatc
      361 tttaaaatag attgtctgat aggacggtat gtataaaacg cttctgtgaa ctgagagaag
      421 gggagaagtt cttgctaaaa tttagttgaa cagccgtatt ccgatactta gataagagat
      481 ctagtcttag atcctactca gttttagggg ataaaaaagg ggcaatagcg attcgagaaa
      541 gattatactc ttcgaaaatc tcttcaaatc acgtcaatat cgccttgccg tatgtgtagg
      601 atactgacta cgtcagttcc atctacaacc tcaaaacagt gttttgagca acctgcggct
      661 agtttcctag tttgatcttt gattttcatt gagtattagt aattcagtta ctaactcgtc
      721 aactctgatt tatccaataa aattgaaaag gatggaaaaa aggataaatt tatgatatac
      781 tttattttga agaccttatt agaaatcttg aaagagtatt gaaaacttag aatgagaaaa
      841 attgttatca atggtggatt accactgcaa ggtgaaatca ctattagtgg tgctaaaaat
      901 agtgtcgttg ccttaattcc agctattatc ttggctgatg atgtggtgac tttggattgc
      961 gttccagata tttcggatgt agccagtctt gtcgaaatca tggaattgat gggagctact
     1021 gttaagcgtt atgacgatgt attggagatt gacccaagag gtgttcaaaa tattccaatg
     1081 ccttatggta aaattaacag tcttcgtgca tcttactatt tttatgggag cctcttaggc
     1141 cgttttggtg aagcgacagt tggtctaccg ggaggatgtg atcttggtcc tcgtccgatt
     1201 gacttacacc ttaaggcgtt tgaagctatg ggtgccactg ctagctacga gggagataac
     1261 atgaagttat ctgctaaaga tacaggactt catggtgcaa gtatttacat ggatacggtt
     1321 agtgtgggag caacgattaa tacgatgatt gctgcggtta aagcaaatgg tcgtactatt
     1381 attgaaaatg cagcccgtga acctgagatt attgatgtag ctactctctt gaataatatg
     1441 ggtgcccata tccgtggggc aggaactaat atcatcatta ttgatggtgt tgaaagatta
     1501 catgggacac gtcatcaggt gattccagac cgcattgaag ctggaacata tatatcttta
     1561 gctgctgcag ttggtaaagg aattcgtata aataatgttc tttacgaaca cctggaaggg
     1621 tttattgcta agttggaaga aatgggagtg agaatgactg tatctgagga cagcattttt
     1681 gtcgaggaac agtctaattt gaaagcaatc aatattaaga cagctcctta cccaggcttt
     1741 gcaactgatt tgcaacaacc gcttacccct cttttactaa gagcgaatgg tcgtggtaca
     1801 attgtcgata cgatttacga aaaacgtgta aatcatgttt ttgaactagc aaagatggat
     1861 gcggatattt cgacaacaaa tggtcatatt ttgtacacgg gtggacgtga tttacgtggg
     1921 gccagtgtta aagcgaccga cttaagagct ggggctgcac tagtcattgc tgggcttatg
     1981 gctgaaggta aaactgaaat taccaatatc gagtttatct tacgtggtta ttctgatatt
     2041 atcgaaaaat tacgtaattt aggagcggat attagacttg ttgaggatta aaccgtagag
     2101 gtgtttatga atatttggac caaattagca atgttttctt tttttgaaac ggatcgcttg
     2161 tatttgcgtc ctttcttttt tagtgatagt caggacttcc gcgagatagc ttcaaatcca
     2221 gaaaatcttc aatttatttt cccaacgcag gcaagtctgg aagaaagtca atatgcactg
     2281 gccaattact ttatgaagtc ccctttggga gtgtgggcaa tttgtgacca gaaaaatcaa
     2341 caaatgattg gttctattaa atttgagaag ttagatgaaa tcaaaaaaga agctgagctt
     2401 ggctattttt tgagaaaaga tgcttggtcg caaggattta tgacagaggt tgttagaaaa
     2461 atttgtcagc tttcttttga ggaatttggc ttaaaacaat tatctatcat tacccacctt
     2521 gaaaatgaag ctagccaaag agttgctctt aagtctggat ttagtttgtt ccgtcagttt
     2581 aagggaagtg atcgttacac aagaaaaatg cgggattatc ttgaatttcg gtatgtaaaa
     2641 ggagagttca atgagtaagc atcaggaaat tctaagctat ttggaggaat taccagtagg
     2701 taaaagggtc agtgttcgta gcatttcgaa tcatctagga gttagtgatg gaacagccta
     2761 tcgggctatt aaagaagctg aaaaccgtgg aattgtggag acccgtccta gaagtggaac
     2821 aattcgtgtt aaatcccaga aagttgctat agagagatta acgtttgctg aaattgcaga
     2881 agtgacttct tctgaggttc tggctgggca agaaggttta gagagagaat ttagtaagtt
     2941 ttcaattggt gccatgactg aacaaaatat cttgtcttac cttcatgatg gggggctctt
     3001 gattgtcgga gaccgaaccc gtattcagtt gctagccttg gaaaatgaaa atgcagttct
     3061 ggttacaggg ggatttcagg ttcatgatga tgtgcttaaa ctggccaatc aaaaagggat
     3121 tcctgttcta agaagtaagc atgatacctt taccgtcgcg accatgatca ataaagcctt
     3181 gtcaaatgtc caaatcaaga ctgatattct gacagttgag aaactttatc gccctagtca
     3241 tgagtatggt tttctgagag agacagatac agttaaagat tatttggact tggttcgtaa
     3301 gaatcgtagc agccgtttcc ctgttatcaa tcaacatcag gtcgttgttg gcgttgtaac
     3361 catgagagac gctggtgata aatcaccaag cacgacaatt gataaggtta tgtctcgtag
     3421 tctatttttg gttggattat cgacaaatat tgccaatgtg agtcaacgga tgatcgcaga
     3481 agactttgaa atggtaccag ttgttcgaag caatcaaact ttgcttggcg ttgtgacgcg
     3541 acgagatgtc atggagaaga tgagccgttc ccaagtttcg gctctaccaa ctttttctga
     3601 gcagattgga caaaagctct cttatcacca tgatgaagta gtcattacag tggaaccctt
     3661 tatgctagaa aaaaatggag ttttggctaa tggtgtattg gcagaaattc tgacccacat
     3721 gacccaagat ttagttgtta atagtggtcg caatctcatt atcgagcaga tgctgatcta
     3781 ctttttgcag gctgttcaga tagatgatat attgcgcatt caggcacgga ttattcatca
     3841 tacgagacgg tcagctataa ttgattacga tatttatcat ggtcaccaga ttgtttcaaa
     3901 agcaaatgtg actgttaaaa ttaattagaa actaggagaa aagatgataa cattaaaatc
     3961 agctcgtgaa atcgaagcta tggacaaggc tggtgatttt ctagcaagta ttcatatagg
     4021 cttacgtgat ttgattaagc caggcgtaga tatgtgggaa gttgaagaat atgtccgccg
     4081 tcgttgtaaa gaagaaaatt tccttccact tcagattggg gttgacggtg ccatgatgga
     4141 ctatccttat gctacctgtt gctctcttaa cgatgaagtg gctcacgctt tccctcgtca
     4201 ttatatcttg aaagatggtg atttgctcaa agttgatatg gttttgggag gtcccattgc
     4261 taaatctgac ctaaatgtct caaaattaaa cttcaacaat gttgaacaaa tgaaaaaata
     4321 cactcagagc tattctggtg gtttagcaga ctcatgttgg gcttatgctg ttggtacacc
     4381 gtccgaagaa gtcaaaaact tgatggatgt aaccaaagaa gctatgtaca agggtattga
     4441 gcaagctgtt gttggaaatc gtatcggtga tatcggtgcg gctattcaag aatacgctga
     4501 aagtcgtggt tacggtgtag tgcgtgattt ggttggtcat ggtgttggcc caactatgca
     4561 cgaagaacca atggttccta actatggtat tgcaggtcgt ggactccgtc ttcgtgaagg
     4621 aatggtctta accattgaac caatgatcaa tacaggcgat tgggaaattg atacagatat
     4681 gaaaactggt tgggcgcata agaccattga cggtggattg tcatgtcagt atgaacacca
     4741 atttgtcatt acgaaagatg gacctgttat cttgactagc caaggtgaag aaggaactta
     4801 ttaataaaaa gtgaaaagac tactggaagt ttattttgat aaaaaatcca gtagatcttt
     4861 tcataataaa acgcattgta tcaagtgtta ggggctgata tcatgcgttt ttctgctttt
     4921 aagatttttt ccaactctgt ttgtaagcgc atcataacaa agggtctagg attcagggct
     4981 ctcctcctat atactattag taaagtaaaa ctaagagagg atattttagt gtcgcagtct
     5041 attgttcctg tagagattcc acaatattgt cgttttgatt ctaaaaagag aaatggaatt
     5101 ctgtttaacg ttcgtattgc caatcttaaa tttacctttt ttagattaga cttcctgcga
     5161 aataaaatat ggtatagtag ttctatgaat gatgaagcaa gtaaacaact aactgatgca
     5221 cgatttaagc gtcttgttgg tgttcagcgc acgacttttg aagagatgtt agctgtatta
     5281 aaaacagctt atcaacttaa acacgcaaaa ggtggacgaa aacctaaatt aatcctagaa
     5341 gaccttctta tggccactct tcaatatgtg cgagaatatc gaacttatga agaaattgtg
     5401 gctgtttttg gtatccacga aagcaactta atccgtcgga gctaatgggt tgaagtaact
     5461 cttgttcaaa gtggttttac gatttcaaga actcctctca gttctgagga cacggtaatg
     5521 attgatgcga cggaagtaca aatcaatcgc cctaaaaaag aattagcgaa ttattctggt
     5581 aaaaagaaat ttcacgctat gaaggctcaa gcgattgtca caagtcaagg gagattgttt
     5641 ctttggatat cactgtgaac tattgtcatg atatgaagtt gttcaaaatg agtcgcagaa
     5701 atatcggaca agctggtaaa atcttggctg acagtggtta tcaagggctc atgaagatat
     5761 atcctcaagc acaaactcca cgtaaatcca gcaaactcaa gccgctaaca gctgaagata
     5821 aagcctgtaa ccatgcgcta tccaaggaga gaagcaaggt tgagaacatc tttgccaaag
     5881 taaaaacgtt taaaatgttt tcaacaacct atcgaaatca tcgtaaacgc ttcggattac
     5941 gaatgaattt gattgctggc attatcaatc atgaactagg attctagttt tgcaggaagt
     6001 ctattgaggt attgagctag tttatgaaaa aattgggtga aaaagtcgag tgttttagaa
     6061 acccacagtg tagtattcta gtttcaatcc actatatttt gctactcccc gtaaagtttc
     6121 tattttccct gatttctgat ataatagaaa tattgacttc aagagtaagg aagagaagat
     6181 gaacgcatta ttaaatggaa tgaatgaccg tcaggctgag gcggtgcaaa cgacagaagg
     6241 tcccttgcta atcatggcag gggctggttc tggaaagact cgtgttttga cccaccgtat
     6301 cgcttatttg attgatgaaa agctggtcaa tccttggaat atcttggcca ttacctttac
     6361 caacaaggct gcgcgtgaga tgaaagagcg tgcttatagc ctcaatccag ctactcagga
     6421 ctgtctgatt gcgaccttcc actccatgtg tgtgcgtatt ttgcgtcgag atgcggacca
     6481 tattggctac aatcgtaatt ttacaattgt ggatcctggt gaacagcgaa cgctcatgaa
     6541 acgtattctc aaacagttga acttggatcc taaaaaatgg aatgaacgaa ctattttggg
     6601 gaccatttcc aatgctaaga atgatttgat tgatgatgtt gcttatgctg cccaagctgg
     6661 cgatatgtat acgcaaattg tggcccagtg ttatacaacc tatcaaaaag aacttcgtca
     6721 gtctgaatcc gttgactttg atgatttgat tatgctgacc ttgcgtctct ttgatcaaaa
     6781 tcctgatgtt ttgacctact accagcaaaa attccaatac atccacgttg atgagtacca
     6841 agataccaac cacgctcagt accaattggt caaactcttg gcttcccgtt ttaaaaatat
     6901 ctgtgtggtt ggggatgcgg accagtctat ctacggttgg cgtggtgctg atatgcagaa
     6961 tatcttggac tttgaaaagg attaccccaa agccaaggtt gttttgttgg aggaaaatta
     7021 ccgctcaacc aaaaccattc tccaagcggc caacgaggtt attaaaaata ataaaaatcg
     7081 ccgtcctaaa aatctctgga ctcaaaatgc tgatggggag caaatcgttt actatcgtgc
     7141 cgatgatgag ctggatgagg ctgtatttgt agccagaacc atcgatgaac ttagtcgcag
     7201 tcaaaacttc cttcataagg attttgcagt tctctatcgg actaatgccc agtcccgtac
     7261 aattgaggaa gccctgctca agtctaacat tccttatacc atggttggtg gaaccaaatt
     7321 ctacagccgt aaggaaattc gcgatattat tgcttatctc aaccttattg ctaatttgag
     7381 tgacaatatt agttttgagc gtattatcaa cgagcctaaa cgtggaattg gtctaggtac
     7441 agttgagaaa atccgtgatt ttgcaaattt gcaaaatatg tctatgctgg atgcttctgc
     7501 taatattatg ttgtctggta tcaagggtaa ggcagcccaa tctatctggg attttgccaa
     7561 tatgatgctt gatttgcggg agcagctaga ccacttaagc attacagagt tggttgagtc
     7621 cgtcctagaa aaaacaggtt atgtcgatat tcttaacgcc caagcgactc tagaaagcaa
     7681 ggcacgggtt gaaaatatcg aagagtttct ttctgttacg aagaactttg atgacaccac
     7741 ggatgtgaca gaagaggaaa ctggtctgga caaactgagt cgtttcttaa atgacttggc
     7801 tttgattgcc gacacagatt caggtagtca ggagacatca gaagtgacct tgatgaccct
     7861 gcatgctgcc aaaggtctcg aatttccagt tgtctttttg attgggatgg aagaaaatgt
     7921 ctttccactt agtcgtgcga ctgaagatcc agatgaatta gaagaagagc gccgtctagc
     7981 ctatgtaggt atcacgcgtg cagagaaaat tctctatctg accaatgcca actcacgctt
     8041 gctttttggt cgtaccaatt ataaccgtcc gactcgtttt attaacgaaa tcagttcaga
     8101 cttgcttgag tatcaaggtc tggctcgtcc tgcaaataca agctttaagg catcatatag
     8161 cagtggtagt atttcctttg gtcaaggtat gagtttggct caggctcttc aagaccgtaa
     8221 acgcggtgct gccccaaaat caatccagtc aagcggtctt ccatttggtc aatttacagc
     8281 tggcgcaaaa ccagcatcta gcgaggcaaa ttggtccatt ggtgatattg ctctccacaa
     8341 gaaatgggga gagggaaccg ttctggaagt ttcaggtagc ggtgctaggc aggaattgaa
     8401 aatcaatttc ccagaagtag gtttgaaaaa acttttagcc agtgtggctc caattgagaa
     8461 aaaaatctaa ttttccatcc ttctcacgaa taataaagtg aggaggattt ttatgtacag
     8521 tatttcattc caagaagatt cactattacc aagagaaagg ctggccaagg aaggagttga
     8581 agcgcttagt aaccaagagt tgctagctat tttactcagg acaggaacac gtcaagctag
     8641 cgtttttgaa attgcccaaa aagtcttgaa caatctttca agcctaacgg atttgaaaaa
     8701 aatgaccctg caggaattgc agagtttgtc tggtattggg cgtgttaagg ccatagaatt
     8761 acaagctatg attgaactgg ggcatcgtat tcacaaacac gagactcttg aaatggaaag
     8821 tattctcagc agtcaaaagt tggccaagaa gatgcagcag gaattagggg ataaaaaaca
     8881 agagcacctg gtggcactct atctcaatac tcaaaatcaa atcatccatc agcagaccat
     8941 ttttatcggt tctgtaactc gtagtatcgc tgaaccgcga gagattcttc actatgcaat
     9001 caagcatatg gcgacttctc ttatcttggt ccacaatcat ccttcaggag cggtagcgcc
     9061 tagccaaaat gatgatcatg tcactaaact tgttaaagaa gcctgcgaac tgatggggat
     9121 tgttctcttg gaccatttga ttgtctctca ttctaattac tttagttatc gtgaaaagac
     9181 agatttaatc taaagttcat taacgacata gtcaaagagt ttcttatctt tgggacgatt
     9241 ttcaaaaaga agttctggat gccattggac accgagaaag gcgacatcat ccgtactcat
     9301 gacagcctca atgataccat ctttaggatc atgagccaca acttttaaat ttggtgctaa
     9361 gtccttgatg ctctggtggt ggaaggagtt gatatgagag atttctccat agatttcttg
     9421 gagaacggta tctggttctg ttaccaagcg ttgagttgtg tactcaacag aagaatcctg
     9481 ccaatggtct tcgatatctt ggtacaaagt tccacccatg gcaacgttaa agagttgggt
     9541 accacggcag acagagaaaa tgggcttttt ctgtttaata gcttccttga tgagggccag
     9601 ttcgaagata tctctttgaa ggtgataatc atcgctatca atggttttgg gttcgccata
     9661 gaattttgga tcgacatttt gcccacctgt caagatgagc ttgtcaatca aactgatata
     9721 gtggcaggcc atttcttgat caccaatcgg taggatgatg ggaatccctc cagcatcttt
     9781 aacgccttca acaaagcctt ttgctgcgta gctcatcatg atgtcatcat ctggatgagt
     9841 tttttcgttt cctgtaatcc caataactgg ttttttcata aaatgatttt cgctttctaa
     9901 tcctcttttc gcatgaagta gaggagggtt tggagttcac ttgtcaaatc gacatactga
     9961 acgaccacgt cttttggtaa atgcagatgg actggtgaaa aactgagaat tcctttcaca
    10021 ccagcatcaa ccaagagatt agcaacctct tgtgacttga cgctgggaac agttaggata
    10081 gcagtcttca catcagcatc cttgatttta tccttgatct gagaaatccc gtaaatggga
    10141 atcccgtcag gagtttgggt accgacttca ggatggtcgt ctaggtcaaa ggccatgata
    10201 atcttcatct tgttacgttc gtggaagcgg tagtggagaa gggcatggcc catatttcca
    10261 ataccaacca gcatgacatt ggtaatagag ttgtcattga gcaaatcggc aaaaaatgtc
    10321 attagttttt tgacatcata gccaaaacca cgacgaccaa gttcaccaaa ataggaaaaa
    10381 tcacgacgta cggtcgctga atcaatccca atggcctctg caatttgctt agagttggca
    10441 cgttcaatct tttctgcatg aaatctctta aaaattcgat agtagagaga gagtcttttt
    10501 gctgtagctt ttggaatagc aaactgttta tctttcacaa aatcacaacc tttctattct
    10561 tctattttat agaaacattg tgaaaaaatc aacaaaaata agaaaaaact aagaaaaatc
    10621 ttagttttga tgtaaaaaat ctgcatgaga tagaaaacgg tagaggtctc cgaccagacc
    10681 ctgataaact ttttggcccc taaaagtcag agaagtcaca taaagtgtat ctggtaaggt
    10741 tacacatcct gacaaagtca acatgagagc ctcatgatcc tcatacttga gagtacgctc
    10801 tacatgatag cagtccttat aggtcagttc aaacattttg gctctatctt tccgattttg
    10861 taaagacacc acgttctacc aagctatcca tgaggaagta gaatttttcc tgatgaatat
    10921 ggtggtcttc tgatttgaaa atatcaacta gacgaaggcc aaacttgtca gtgatattga
    10981 ttttagcccc tgtaagttcc ttgttaatga tgattttgag ttggaagcct tcaccgctgt
    11041 ttggcacttt ttccaaaagg cgagtcagtt catagttacc aaccttagtt tcaaaaaagg
    11101 tgttatcttt gagggtgaat tttttaacag aagggctaag agtgtaatcg taacgacaat
    11161 tttttaactg aatgattttt tcaaatgcca tatggctaac ctccgataat ttcttttaag
    11221 gtttttgcga gggtttgtag gtcttcaacg gtattttgtg gcgacaaact gatgcgaagg
    11281 gattccttca agcgttctga atttgcgcca tacatggctt caagaacatg gctggattgg
    11341 acaacgcctg cagtacaggc tgagccagta gagattgaaa ttccagctaa atctagccga
    11401 aggagtaaga ggtcattttt ctgaccagga aatccaatat tgagaacata agggagatga
    11461 tgttttcctc tattcaggta atactgaatg ccctccagct ctgccagaaa ggcagtttct
    11521 agattttgta catgttgaaa atgttcttct tgtttttcta ggtcttcttt tagggctgca
    11581 accatgccta cgatggcagg cagattttca gttcctgcac gttttttctg ttcctggtct
    11641 ccgccatgta gataggaatc aaagtccatg ctagatgcgt agagaaaacc gattccctta
    11701 ggaccatgga atttgtgggc agaagcagtg agaaaatcaa tgcccaattc ttctgaatga
    11761 attgggattt taccaatagc ctgaactgca tcaacatgat aggcagcagg gtgttgcttg
    11821 agtatttggc caatttcagc gatgggcagt aggtttcctg tctcattatt ggcaaacatg
    11881 gtagaaacca aaatcgtatc gtcacgtaaa gccttttgaa tttgctgggc tgtgatttct
    11941 tgattttctg gctggataat ggttgcttca aacccaaagt gttgaaccaa gtaatcaatt
    12001 gtttcaagga cagcatggtg ctcgatggca gttgtgatga tatgttttcc ttgttcttgg
    12061 tgacgaagac agtagccaat gatggtagta ttattgcctt cagtcccacc agaagtgaaa
    12121 aagatatgtt gaggttttgt ccttagtaac tgggctagtt cctgacgggc ttctcgcaag
    12181 agtttgccag cttgacgacc atgaccatga atactagaag gatttccgtg ggtttcttgc
    12241 ataaccttgg tcatagctga aatagcaact gctgacatag gagtcgttgc agcattgtcc
    12301 aaataaatca aagaatcacc ttatttcttt ttattgtagg caaagagtgg gctgactggt
    12361 tttctttcgt gaatacggac gatagcatca ccaattaact cactagcagt gatgtagcat
    12421 acatttttag gagttttttc ttttgttgct actgaatcag tcacaagaat ttctttaata
    12481 ttagtattgt caagaagctc agcagctccc tcgacgaaga gaccgtggct agaaacagca
    12541 taaatttctg tagctccttc acgttcaacg attttagaag cttcagagaa ggtacgtcct
    12601 gtatttaaaa tatcatcaat caagatagct ttcttacctt caacatcacc aataatataa
    12661 ccttcgttac gagttgcatc gtcttgaggg tagtcgataa tggcgatagg agcatcaaga
    12721 tattcagcca ggctacgcgc acgtttgaca cctgaatttt tagggctaac gacaacaaca
    12781 tctgaaccaa gcaatccttt atcgcagtaa tgttttgcga ataggggaac agtgaaaaga
    12841 ttatccactg gaatatcaaa gaaaccttga acctgaacgg catgcaaatc aagagtcagg
    12901 atacgatcaa ctccagcctt aaccagcata ttggcaacta gttttgctgt aagtggctca
    12961 cgaggacaag caatgcggtc ttgacgtgca tagccaaaat atggaaggac aacgttgata
    13021 ctgtgggcac ttgcacgcac acaagcatcg accatgatta acaattccat taggtggttg
    13081 ttgacaggga aacttgttga ttggatgatg taaacatcat aaccacggac actttcttcg
    13141 atatttactt ggatttctcc gtctgaaaat tgacgtgatg atagttttcc aagtgggaca
    13201 ccaacagctt gggcaatttt ttgtgcaatc tcttggttag agttgagtgc gaaaagtttc
    13261 atgttttttc tatctgacat tatagaccgt cctctgtaaa ctttataaat cctagttata
    13321 tttaccttac atatatgaac tgggatttgt gtatttttat cttttctatt ttaccaaaaa
    13381 atggagatta tttcagctat ttttcatact tttgacaaat cgaaccaatt ttgaaggagc
    13441 tttttggtag gaaatctgat ttttctctaa aaattgtcgg aaatcctgtt tgccttgctc
    13501 atgattttcc acttcaagct ccaattcgta atctgttata tcaaagtatc ggctctgatc
    13561 cagtgccatg agaccaatag ctgttttcat ttcatagcga agcgttgtta gacaaccaag
    13621 aacctgccag ttcttacttt ggataccatg tttcgccaat tcatccagta ctagcccttg
    13681 aggaagttct tccttactca gatagttctc agcatctttt agttgcaatt tttggttgta
    13741 ttccatgttt ccaacactct gcgggacttt gagtgtcaac tcagcccagt cttcaaaggt
    13801 tcgaatgcgc atagcgactt tcttttctcg cagttcaaaa tcaggcgtgt cgatgtagta
    13861 atttgtttga agaacaggag tgacacctgt gaactggtct tttagacgat tgtattcatc
    13921 ttttttcaat agtgttttca attcaatttc taaatgtttc atttttctta ccttttttta
    13981 tcgttgaaag cggatttatg gtataataag cattgtattt attgtatatg aatctggaga
    14041 aaaaatcaaa gatatttttg acggataata tgagaacaag ggagaatata tgaccttaga
    14101 atgggaagaa tttctagatc cttacattca agctgttggt gagttaaaga ttaaacttcg
    14161 tggtattcgt aagcaatatc gtaagcaaaa taagcattct ccaattgagt ttgtgaccgg
    14221 tcgagtcaag ccaattgaga gcatcaaaga aaaaatggct cgtcgtggca ttacttatgc
    14281 gaccttggaa cacgatttgc aggatattgc tggcttacgt gtgatggttc agtttgtaga
    14341 tgacgtcaag gaagtagtgg atattttgca caagcgtcag gatatgcgaa tcatacagga
    14401 gcgagattac attactcata gaaaagcatc aggctatcgt tcctatcatg tggtagtaga
    14461 atatacggtt gataccatca atggagctaa gactattttg gcagaaattc aaattcgtac
    14521 tttggccatg aatttctggg caacgataga acattctctc aactacaagt accaagggga
    14581 tttcccagat gagattaaga agcgactgga aattacagct agaatcgccc atcagttgga
    14641 tgaagaaatg ggtgaaattc gtgatgatat ccaagaagcc caggcacttt ttgatccttt
    14701 gagtagaaaa ttaaatgacg gtgtaggaaa cagtgacgat acagatgaag aatacaggta
    14761 aacgaattga tctgatagcc aatagaaaac cgcagagtca aagggttttg tatgaattgc
    14821 gagatcgttt gaagagaaat cagtttatac tcaatgatac caatccggat attgtcattt
    14881 ccattggcgg ggatggtatg ctcttgtcgg cctttcataa gtacgaaaat cagcttgaca
    14941 aggtccgctt tatcggtctt catactggac atttgggctt ctatacagat tatcgtgatt
    15001 ttgagttgga caagctagtg actaatttgc agctagatac tggggcaagg gtttcttacc
    15061 ctgttctgaa tgtgaaggtc tttcttgaaa atggtgaagt taagattttc agagcactca
    15121 acgaagccag catccgcagg tctgatcgaa ccatggtggc agatattgta ataaatggtg
    15181 ttccctttga acgttttcgt ggagacgggc taacagtttc gacaccgact ggtagtactg
    15241 cctataacaa gtctcttggc ggtgctgttt tacaccctac cattgaagct ttgcaattaa
    15301 cggaaattgc cagccttaat aatcgtgtct atcgaacact gggctcttcc attattgtgc
    15361 ctaagaagga taagattgaa cttattccaa caagaaacga ttatcatact atttcggttg
    15421 acaatagcgt ttattctttc cgtaatattg agcgtattga gtatcaaatc gaccatcata
    15481 agattcactt tgtcgcgact cctagccata ccagtttctg gaaccgtgtt aaggatgcct
    15541 ttatcggtga ggtggatgaa tgaggtttga atttatcgca gatgaacatg tcaaggttaa
    15601 gaccttctta aaaaagcacg aggtttctaa gggattgctg gccaagatta agtttcgagg
    15661 tggagctatt ctggtcaata atcaaccgca aaatgcaacg tatctattgg acgttggaga
    15721 ctacgttatc attgacattc ccgctgagaa aggctttgaa accttggagg ctattgagct
    15781 tccattagat attctctatg aggatgacca ctttctagtc ttgaataaac cctatggagt
    15841 ggcttctatt cctagtgtca atcactctaa taccattgcc aattttatca agggttacta
    15901 tgtcaagcaa aattatgaaa atcagcaggt tcacattgtt accagactag atagggacac
    15961 ttctggcttg atgctctttg ccaagcacgg ttatgcccat gcacgattag acaagcagtt
    16021 gcagaagaaa tctatcgaga aacgctactt tgctttggtt aagggagatg gacatttgga
    16081 gccagaaggg gaaattattg ctccgattgc gcgtgatgaa gattccatta ttaccagacg
    16141 agtggctaaa ggcggaaagt atgcccatac ttcatacaag attgtagctt cttatggaaa
    16201 tattcacttg gtctatattc acctgcacac tggtcgaacc catcaaatcc gagtccattt
    16261 ttctcatatc ggttttcctt tgctgggaga tgatttgtat ggtggtagtc tggaagatgg
    16321 tattcaacgt caggctctgc attgccatta cctatccttt tatcatccat ttttagagca
    16381 agacttgcag ttagaaagtc ccttgccgga tgatttcagt aaccttatta cccagttatc
    16441 aactaatact ctataaaaac tgtctcagag tataattatt atcttaaagg agaaaactca
    16501 tggaagtttt tgaaagtctc aaagccaacc ttgttggtaa aaatgctcgt atcgttctcc
    16561 ctgaagggga agagcctcgt attcttcaag caacaaaacg cttagtaaaa gaaacagaag
    16621 tgattcctgt tttgcttgga aatcctgaaa aaattaaaat ttatcttgaa attgaaggaa
    16681 tcatggatgg ttatgaggtc atcgaccctc aacattatcc tcaatttgaa gaaatggttt
    16741 ctgccttggt ggagcgtcgc aagggcaaaa tgactgaaga agatgtacgc aaggttttgg
    16801 ttgaagatgt caactacttt ggtgtgatgt tggtttactt gggcttggtt gatggaatgg
    16861 tgtcaggagc gattcactca acagcttcaa cagttcgtcc agctctacaa atcatcaaaa
    16921 ctcgtccaaa tgtaactcgt acttcaggag ccttcctcat ggttcgtggt acggaacgtt
    16981 acctatttgg agactgtgcc attaacatca atccagatgc agaagccttg gctgaaattg
    17041 ccatcaactc agcaatcaca gctaagatgt ttggcatcga acctaaaatt gccatgttga
    17101 gctattctac taaaggttca gggtttggtg aaagcgttga taaggtcgtt gaagcaacta
    17161 aaattgctca cgacttgcgt cctgaccttg aaatcgatgg tgagttgcaa tttgatgcag
    17221 cctttgttcc tgaaactgca gctctgaaag ctcctggaag tacggtagct ggtcaagcaa
    17281 atgtcttcat cttcccaggt atcgaggcag gaaatattgg ttacaagatg gctgaacgtc
    17341 ttggtggttt tgcggctgta ggacctgttt tgcaaggttt gaacaaacca gttaacgacc
    17401 tttctcgtgg atgtaatgca gacgatgttt acaagttgac cctcatcaca gcagctcaag
    17461 cagttcatca atagtgaaaa ctataaagtg atatactatg ctatactgta gttatgaaac
    17521 tatgtacgaa aagcactgcc attaattcct gagaactaaa ttactgattg gtgtcaaaaa
    17581 ggaaaacttc catgcgatga tatcctgtct atacacgacc tatagaaatc tgtaatatac
    17641 atgtccgtaa aacgataaat tccctttttg attttaaatg agtatgaaaa gagaattttt
    17701 catgagaaaa cgcataatat tgagtgcttt gacacctgct attatgcgtt ttttataatt
    17761 tttgcacctt tcttagcctt tgttatcgac aagcattgtt ctagctcctt gtttttagaa
    17821 aggtaatttc ccagcgaaag cacccagttt tttcttggta gtttcatcga tttgttcaag
    17881 agcagagttg atggcttgaa cggtcatatc agaaagagtc tcaaggtcct ctgggtcaac
    17941 gacagctgga ttgaaatcaa tgctgacaac tttcttatcg ccagttaagg tcgcttggac
    18001 aagatcttga gcagatttgc caacaaattg catagcagca agttcagctt ggctttgttc
    18061 catttgtttt tgaagttttt gtgcttgacg catcatgttt tgcatgttca tcataagatt
    18121 tctaaagttc ctttcatctc aaggggataa tccttgttgt taacaagtgc ctgattttct
    18181 ctcctaattg ttattagtag catagccatc aagcccaagt attgatttta ttataccatt
    18241 tttttaagac atagtctttt attggtgaga gttatttatg tcaaggagtg attagaaata
    18301 aattaaaata atgatttttg ctaagagggc tctattctag tttcatggaa atgattttac
    18361 taaaggttcg tatgctttgt ttgcaaa
//
LOCUS       DAVLVP010000032        17912 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.032, whole genome shotgun sequence.
ACCESSION   DAVLVP010000032 DAVLVP010000000
VERSION     DAVLVP010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17912)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17912)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17912
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.032"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(106..658)
                     /locus_tag="VYN69_001053"
                     /pseudo
     CDS             complement(106..658)
                     /locus_tag="VYN69_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007897432.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus and C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(734..1324)
                     /locus_tag="VYN69_001054"
                     /pseudo
     CDS             complement(734..1324)
                     /locus_tag="VYN69_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457791.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1248..1442)
                     /locus_tag="VYN69_001055"
     CDS             complement(1248..1442)
                     /locus_tag="VYN69_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1533341.1"
                     /translation="MKIIQQQSATIDSLTNELALLREQVAYLTQKLYGKSSEKSVCPS
                     GQLNLFEEESPSEEDGDVPS"
     gene            complement(1639..1962)
                     /gene="tnpB"
                     /locus_tag="VYN69_001056"
     CDS             complement(1639..1962)
                     /gene="tnpB"
                     /locus_tag="VYN69_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEV1533342.1"
                     /translation="MTIHLSSLGQVYLVCGKTDMRQGIDSLAYLVKTHFELDPFSGQI
                     FLFCGGRKDRFKVLYWDGQGFWLLYKRFENGRLTWPSTEKDVKALAPEQVDWLMKGFS
                     ITPKI"
     gene            complement(1943..2128)
                     /locus_tag="VYN69_001057"
     CDS             complement(1943..2128)
                     /locus_tag="VYN69_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001136123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533343.1"
                     /translation="MPQPIVPVEIPQSRRFDSKKRNDILLKIRIGKLEVSFFQSLNLE
                     MIEQLLDKVLLYDNSSI"
     gene            complement(2430..3992)
                     /gene="guaA"
                     /locus_tag="VYN69_001058"
     CDS             complement(2430..3992)
                     /gene="guaA"
                     /locus_tag="VYN69_001058"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000065723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEV1533344.1"
                     /translation="MSNISTDLQDVEKIIVLDYGSQYNQLISRRIREIGVFSELKSHK
                     ISAAEVREVNPVGIILSGGPNSVYEDGSFDIDPEIFELGIPILGICYGMQLLTHKLGG
                     KVVPAGDAGNREYGQSTLTHTPSALFESTPDEQTVLMSHGDAVTEIPADFVRIGTSAD
                     CPYAAIENPDKHIYGIQFHPEVRHSVYGNDILRNFALNICKAKGDWSMDNFIDMQIKK
                     IRETVGDKRVLLGLSGGVDSSVVGVLLQKAIGDQLICIFVDHGLLRKGEADQVMDMLG
                     GKFGLNIVKADAAKRFLDKLAGVSDPEQKRKIIGNEFVYVFDDEASKLKDVKFLAQGT
                     LYTDVIESGTDTAQTIKSHHNVGGLPEDMQFELIEPLNTLYKDEVRALGTELGMPDHI
                     VWRQPFPGPGLAIRVMGEITEEKLETVRESDAILREEIAKAGLDRDIWQYFTVNTGVR
                     SVGVMGDGRTYDYTIAIRAITSIDGMTADFAKIPWEVLQKISVRIVNEVDHVNRIVYD
                     ITSKPPATVEWE"
     gene            4134..4832
                     /locus_tag="VYN69_001059"
     CDS             4134..4832
                     /locus_tag="VYN69_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000936186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1533345.1"
                     /translation="MLPAYIKIHDQIKKDIDEYRWAIGERLPSERDLAEQFAVSRMTL
                     RQAVSLLVEEGVLERRVGSGTFVSSTRVQEKMRGTTSFTEIVKSQGKVPSSQLISYKK
                     TIPNEQEVAKLGIFPTDNIIRMERVRYADQVPLVYEVASIPEKFIKDFKKEEITSHFF
                     QTLQKHGYRIGKSQQTIYARLAKEKIAHYLEVEKGHAILGLTQVSYLEDGTAFEYVKS
                     QYVGERFEFYLENN"
     gene            complement(4875..5771)
                     /locus_tag="VYN69_001060"
     CDS             complement(4875..5771)
                     /locus_tag="VYN69_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000071673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HEV1533346.1"
                     /translation="MSNSLKGTLLTVVAGIAWGVSGTSGQYLMAHGISALVLTNLRLL
                     IAGGILMILAYATAKDKILVFLKDRKSLLSLLIFALIGLFLNQFAYLSAIQETNAGTA
                     TVLQYVCPVGILIYSCIKDRVAPTLGEIVSIIFAIGGTFLIATHGQLDQLSMTPAGLF
                     WGLFSALTYALYIILPIALIKKWGSSLVIGVGMVIAGLVALPFTGVLQANIPTSLDFL
                     LAFAGIILIGTVFAYTAFLKGASLIGPVKSSLLASIEPISAVFFAFLIMNEQFYPIDF
                     LGMAMILFAVTLISLKDLFLEK"
     gene            complement(5780..6715)
                     /locus_tag="VYN69_001061"
     CDS             complement(5780..6715)
                     /locus_tag="VYN69_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEV1533347.1"
                     /translation="MKWTKIIKKIEEQIEAGIYPGASFAYFKDNQWTEFYLGQSDPEH
                     GLQTEAGLVYDLASVSKVVGVGTVCTFLWEIGQLDIDRLVIDFLPESDYPDITIRQLL
                     THATDLDPFIPNRDLLTAPELKEAMFHLNRRSQPAFLYSDVHFLLLGFILERIFNQDL
                     DVILKDQVWKPWGMTETKFGPVELAVPTVRGVEAGIVHDPKARLLGRHAGSAGLFSTI
                     KDLQIFLEHYLADDFARDLNQNFSPLDDKERSLAWNLEGDWLDHTGYTGTFIMWNRQK
                     QEATIFLSNRTYEKDERAQWILDRNQVMNLIRKEE"
     gene            complement(6712..7437)
                     /locus_tag="VYN69_001062"
     CDS             complement(6712..7437)
                     /locus_tag="VYN69_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CppA family protein"
                     /protein_id="HEV1533348.1"
                     /translation="MNVNQIVRIIPTLKANNRKLNETFYIETLGMKALLEESAFLSLG
                     DQTGLEKLVLEEAPSMRTRKVEGRKKLARLIVKVENPLEIEGILSKTDSIHRLYKGQN
                     GYAFEIFSPEDDLILIHAEDDIASLVEVGEKPEFQTDLASISLSKFEISMELHLPTDI
                     ESFLESSEIGASLDFIPAQGQDLTVDNTVTWDLSMLKFLVNELDIASLRQKFESTEYF
                     IPKSEKFFLCKDRNNVELWFEEV"
     gene            complement(7521..8156)
                     /locus_tag="VYN69_001063"
     CDS             complement(7521..8156)
                     /locus_tag="VYN69_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-alkyl-2-acetylglycerophosphocholine esterase"
                     /protein_id="HEV1533349.1"
                     /translation="MVVQLLENWLLKEQEKIQTKYRHLNHISVVEPNILFIGDSIVEY
                     YPLQELFGTSKTIVNRGIRGYQTGLLLENLDAHLYGGAVDKIFLLIGTNDIGKDVPVN
                     EVLNNLEAIIQSVARDYPLTEIKLLSILPVNEGEKYQQAVYIRSNEKIQNWNQAYQEL
                     ASAYMQVEFVPVFDCLTDQAGQLKKEYTTDGLHLSIAGYQALSKSLKDYLY"
     gene            complement(8158..8985)
                     /locus_tag="VYN69_001064"
     CDS             complement(8158..8985)
                     /locus_tag="VYN69_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1533350.1"
                     /translation="MMIKVIATDMDGTLLDARGQLDLPRLEKILDQLDQRGIRFVIAT
                     GNEIHRMRQLLSPLVDRVVLVVANGARIFENNELIQAQTWDDAIVNKALAHFKGRACQ
                     NQFVVTGMKGDFVKEGTIFTDLESFMTPEMIEKFYQRMQFVDELTSDLFGGVLKMSMV
                     VGEERLSSVLEEINALFDGRVRAVSSGYGCIDILQAGIHKAWGLEELLKRWDLKSQEI
                     MAFGDSENDVEMLEMAGIAYAMENADEKAKAVATALAPANSQGGVYQVLENWLEKGE"
     gene            complement(9194..9343)
                     /locus_tag="VYN69_001065"
     CDS             complement(9194..9343)
                     /locus_tag="VYN69_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533351.1"
                     /translation="MGNFISNQRIESMGDEENAKWTERGVLMDVTIKKKDGKTTIGTA
                     KAHPT"
     gene            complement(9344..10060)
                     /locus_tag="VYN69_001066"
     CDS             complement(9344..10060)
                     /locus_tag="VYN69_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HEV1533352.1"
                     /translation="MAHGDLLYHDGLFFSAKKEDGTYDFHENFEYVTPWLKQADLAIG
                     DFEGTINKDHYLAGYLLFNAPAEVMDAIKEAGYHVLDLAHNHILDSQIEGVISTADII
                     EKAGITPIGVYTHEPRVQAPLVIKEVNGIKVALLAYSYGFNGIEQYISQEDYNRYLLD
                     LNEDKMKAEVERAEKEADITIIMPLMGVEYRLEPTEEQKALYHKMIDWGADIIFGGHP
                     HVVEPSETVEKDGDKKLIVY"
     gene            10573..11184
                     /locus_tag="VYN69_001067"
     CDS             10573..11184
                     /locus_tag="VYN69_001067"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015058782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEV1533353.1"
                     /translation="MSAIERITKAAHLIDMNDIIREGNPTLRTVAEEVTFPLSDQEII
                     LGEKMMQFLKHSQDPVMAEKMGLRGGVGLAAPQLDISKRIIAVLVPNIVEEGETPQEA
                     YDLEAIMYNPKIVSHSVQDAALGEGEGCLSVDRNVPGYVVRHARVTVDYFDKDGEKHR
                     IKLKGYNSIVVQHEIDHINGIMFYDRINEKDPFAVKDGLLILE"
     gene            11229..11957
                     /gene="rlmB"
                     /locus_tag="VYN69_001068"
     CDS             11229..11957
                     /gene="rlmB"
                     /locus_tag="VYN69_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006151108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEV1533354.1"
                     /translation="MKTNDIVYGVHAVTEALLANTGNKLYLQEDLRGKNVEKVKELAT
                     EKKVSISWTSKKSLSEMTEGAVHQGFVLRVSEFAYSELDYILAKTRQEENPLLLILDG
                     LTDPHNLGSILRTADATNVSGVIIPKHRAVGVTPVVAKTATGAIEHVPIARVTNLSQT
                     LDKLKDEGFWTFGTDMNGTPCHKWNTKGKIALIIGNEGKGISSNIKKQVDEMITIPMN
                     GHVQSLNASVAAAILMYEVFRNRL"
     gene            complement(11995..12906)
                     /gene="trxB"
                     /locus_tag="VYN69_001069"
     CDS             complement(11995..12906)
                     /gene="trxB"
                     /locus_tag="VYN69_001069"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEV1533355.1"
                     /translation="MYDTIIIGAGPAGMTAALYAARSNLKVALIEGGLPGGQMNNTSD
                     IENYPGYANISGPELAEKMFEPLENLGVEHIYGYVENVEDHGDFKKVMTDDQTYETRT
                     VIVATGSKHRPLGVPGEEELNSRGVSYCAVCDGAFFRDQDLLVVGGGDSAVEEALFLT
                     RFAKTVTIVHRRDQLRAQKVLQDRAFANEKISFIWDSVVREIKGENRVESVVFENVKT
                     GQVTEQVFGGVFIYVGLDPLSDFVKELNIQDQAGWIVTDNHMKTAVDGIFAVGDVRLK
                     DLRQVTTAVGDGAIAGQEAYKFITEHS"
     gene            complement(12972..13196)
                     /locus_tag="VYN69_001070"
     CDS             complement(12972..13196)
                     /locus_tag="VYN69_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4059 family protein"
                     /protein_id="HEV1533356.1"
                     /translation="MLLQLFSLYFESLILTTILVLIFLGIWIGLRAMSGVDKTARARQ
                     AHLYDMIMIGVLVVPVLSFAVMSLILVFKA"
     gene            complement(13274..14017)
                     /locus_tag="VYN69_001071"
     CDS             complement(13274..14017)
                     /locus_tag="VYN69_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1533357.1"
                     /translation="MIKISNLSKSFSGQTVLDHLNLDIQKGEVVALIGSSGAGKSTFL
                     RSLNYLETPDSGSIQIDGFSVDFSKITQEEILALRRKLSMVFQQFNLFERRTALDNVK
                     EGLVVVKKLSDQEATKIAKEELAKVGLSDRENHYPRHLSGGQKQRVALARALAMKPDV
                     LLLDEPTSALDPELVGEVEKSIADAAKSGQTMILVSHDMSFVAQVADKILFLDKGKII
                     ESGTPDEIIHTPKEERTKEFFASYKRTYI"
     gene            complement(14017..14817)
                     /locus_tag="VYN69_001072"
     CDS             complement(14017..14817)
                     /locus_tag="VYN69_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001103446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1533358.1"
                     /translation="MNVTTILASDWYQNLMQLIPDGKLFSLRSVFDGIPRIVQQLPTT
                     IMLTIGGALFGLVLALLFAIVKINRVKILYPLQAFFVSFLKGTPILVQLMLTYYGIPL
                     ALKALNQQWGTGLNINAIPAAAFAIVAFAFNEAAYASETIRAAILSVNPGEIEAARSL
                     GMTRAQVYRRVIIPNAAVVATPTLINSLIGLTKGTSLAFSAGVVEVFAQAQILGGADY
                     RYFERFISVALVYWVVNIGIESLGRFIERKMAISAPDTVQTDVKGDLR"
     gene            complement(14975..15331)
                     /locus_tag="VYN69_001073"
     CDS             complement(14975..15331)
                     /locus_tag="VYN69_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEV1533359.1"
                     /translation="MLEFIEYPKCSTCKKAKQELNQLGVDYKAVHIVEETPSQEVILN
                     WLETSGFELKQFFNTSGIKYRELGLKDKVGSLSNQEAAELLASDGMLLKRPILVENGT
                     VKQIGYRKSYEELGLK"
     gene            complement(15325..15855)
                     /locus_tag="VYN69_001074"
     CDS             complement(15325..15855)
                     /locus_tag="VYN69_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEV1533360.1"
                     /translation="MQKKYVKILYSSPIGILSLVADDHYLYGIWVQEQKHFERGLGDE
                     TIEEVVSHPILDPVIACLDDYFKGKPQDLSNLLLAPIGTNFEKRVWDYLQGIPYGQTV
                     TYGQIAQDLQVASAQAIGGAVGRNPWSILVPCHRVLGAGKRLTGYAAGVEKKAWLLEH
                     EGVDFKDRNNRRRSTC"
     gene            complement(15855..16349)
                     /locus_tag="VYN69_001075"
     CDS             complement(15855..16349)
                     /locus_tag="VYN69_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1533361.1"
                     /translation="MEIRLAFPNEVDAIMQVMEDAKKCLADAGSDQWQNGYPNADVII
                     DDIISGQAYVALEEGELLAYAAVTKSPEEAYEAIYEGNWQAGESEYLVFHRIAVAADV
                     QGKGVAQTFLEGLIEGFDYLDFRSDTHAENKVMQHIFEKLGFKQVGKVPVDGERLAYQ
                     KLKK"
     gene            16484..16930
                     /locus_tag="VYN69_001076"
     CDS             16484..16930
                     /locus_tag="VYN69_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000858725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEV1533362.1"
                     /translation="MKTTFSYPKWAEIPNIDLYLDQVLLYVNQVCAPISPNKDKGLTA
                     SMVNNYVKNGYLTKPDKKKYQRQQIARLIAITTLKSVFSIQEIAQTLNTLQTQASSDQ
                     LYDAFVDYMNQGIDPANPIIQTSCQTVKLYHQTLDLIDHTQEEVIQ"
     gene            16927..17574
                     /locus_tag="VYN69_001077"
     CDS             16927..17574
                     /locus_tag="VYN69_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001097995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEV1533363.1"
                     /translation="MNTSLKLSKQLSFGEEIANSVTHAVGAVIMLILLPISSIYSYEA
                     HGFLSSIGVSIFVISLFLMFLSSTIYHSMAYGSTHKYVLRIIDHSMIYVAIAGSYTPV
                     VLTLMNNWFGYLIIVIQWGTTIFGILYKIFAKKVNEKFSLALYLIMGWLVLAIIPAII
                     SQTTPVFWSLMVTGGLCYTVGAGFYAKKKPYFHMIWHLFILAASALQYIAIVYYM"
BASE COUNT         5379 a         4017 c         3194 g         5322 t
ORIGIN      
        1 tataatggat tgaaataaga tatgaacaaa tcgattagga aaggaaaatt gatttataga
       61 aatattttag cagtcgatgc gtactgttat agattcaacg cactataatt gatgacgttt
      121 agctgtttcc aacaaactca taataatagc tcttgcttta gctccttcaa aactttgaga
      181 aaataaccac cctcgctttt taaaatttag cttaggctaa aagagtccac tggactcttt
      241 tactccgtcc cataaccaat gatttaatgg cgcgttcagc tagattattg gaaaggacca
      301 gatgtccgtc tttcaaaatg gtcttaaagg tttcttcata cttgaggctg tattcaattg
      361 cccttcctag ttttgaacct gctaaaactg actgacgccg gcactaagca aagaagtctt
      421 ccattaaggg ctggagatgt tcttgacgtt tctgtagtcg ttcatcagct ggcaaagcct
      481 cccagtctct ttccaaggaa aataactgat cacaataagc taaaccttta gctcctaagg
      541 atgatttatc tgcttgcttg ggggttgctt caaaaaattt ccttctcaca tgcgcccaac
      601 atccaacaag ttcagcttct gccagttgac gataagcttc tagcggctcg cagtttacca
      661 agcttagcgt cgccttgctc ctaaaccttg gacttctatc gcataggcag aactagagga
      721 ctaaagtcct aagttactgc cgcaacatat cacaatgaac atagccagaa taatctccta
      781 ggaattcttg tactaccgaa ccactccgac tctgatcatg atggtacagc gtgattgctt
      841 gattctcaac tttcccagac aaaaaggtcc aatagtaggt cagatggcta tgactctcta
      901 gaacccgata agaggtttca tctgcatgaa gtaagggctt gagtcaatag tctcgttata
      961 aaggggcttt aggaatagga gctttcacga ttttatcact cggattttta tcactgcatg
     1021 cttggcattt gtaagcgtgt tggatatgat ctatgcgttt taattgcgca ggaataaaga
     1081 ccaattcttg tccttgaaga ctccctccaa tctcttttag atctccctga caatcagggc
     1141 aaatcctctc ttctacttga tgatgaactt cttctgaatc aaattggaca agaagagctt
     1201 ggcgtttccc tttagatttc ttacgtttgt aggtaatttc ctctgtttca actgggaaca
     1261 tctccatctt cctcagatgg agactcttcc tcaaaaaggt tgagttgtcc agaggggcaa
     1321 acacttttct cagaggattt tccatagagc ttttgagtca gataagccac ttgttcacgc
     1381 agaagggcaa gttcattggt gagactatca attgtagcac tctgttgttg aatgattttc
     1441 aatcttcttc catgagcttc cctctctgtt ttctaacttt attatactaa aaagaaagtc
     1501 ctcatttcaa tagaaatcac gactttctga tggatttata gtaaaatgaa ataataacag
     1561 gacaaatcga tcaggacagt aaaatcgatt tctaacaatg ttttagaagc agaggtgtac
     1621 tattctagtt tcaatctact atatttttgg agtgatagaa aagcctttca tcagccaatc
     1681 tacttgttca ggtgcgagag ctttgacatc cttttctgta ctgggccaag tcagtctgcc
     1741 gttctcaaag cgtttatata gtagccaaaa tccttgacca tcccagtaaa ggactttaaa
     1801 gcggtcttta cgtccaccac aaaagagaaa gatttgaccg gagaaaggat ccaattcaaa
     1861 gtgggtttta acgagataag ccagtgaatc gattccttgt ctcatatcag ttttcccaca
     1921 cacgagatag acctgcccta ggctagatag atgaattgtc atagagcaac accttatcca
     1981 aaagctgttc tatcatttcg agattgagag attgaaaaaa acttacttca agcttgccaa
     2041 tacgaatttt aagtagaata tcatttctct ttttagaatc aaaacgacga gattgtggaa
     2101 tctctacagg aacaataggt tgtggcatta aaatatcctc caatatttac tttactaata
     2161 gtatacagga ggagctccat gaatgataga taccttgtta tgacgcgctt acgatattag
     2221 aaagttggct ctctaattaa attaattctc attatatata tcaaaagacc gcagtttgaa
     2281 aactacggtc taattttttg tccaaatgta aatcacttgg aactattatt caatttattc
     2341 tttttagtaa atgttcccgt tttaatccca gttacagttt ttaatcctct gtaaccgttg
     2401 attttacaaa gcttttaatt tttttgcgat tactcccact caactgttgc aggtggttta
     2461 cttgtaatat cgtagacgat acggttaaca tgatccactt catttacgat acgtactgag
     2521 attttttgaa gtacttccca tggaattttg gcaaaatcag cagtcatacc atcgatagaa
     2581 gtgatagcac ggattgcaat cgtgtagtca tacgtacgac cgtcacccat aacaccgact
     2641 gaacgaacgc ctgtgttaac agtgaagtat tgccaaatat cgcggtcaag tccagcttta
     2701 gcgatttctt cacgaagaat agcgtctgat tcacgaacgg tttcaagttt ctcttcagtg
     2761 atttcaccca tgacacggat agcaagtcct ggtcctggga atggttggcg ccatacgata
     2821 tggtctggca taccaagctc tgtaccaaga gcacgaactt catccttgta aagagtattg
     2881 agtggttcaa tcaattcaaa ctgcatatct tctggaagac cacccacgtt gtggtgtgac
     2941 ttgatagttt gagctgtatc cgtaccagac tcgataacat ctgtatataa agtaccttga
     3001 gcaaggaatt tcacatcttt gagcttgctt gcttcgtcat cgaatacata gacaaactcg
     3061 ttaccgatga ttttacgttt ttgttcagga tcagaaacgc cagcaagttt gtcaaggaaa
     3121 cgtttagcag cgtctgcttt gacgatattc aaaccaaact taccaccgag catgtccata
     3181 acttgatcag cttcgccttt acgaagaagg ccgtggtcta cgaagataca gatcaattga
     3241 tcgccaatcg ctttttggag aagaacccca acgacagatg agtcaacacc acctgataga
     3301 ccaagaagga cacgtttatc accgacggtt tcacgaattt ttttgatctg catgtcaatg
     3361 aaattatcca ttgaccagtc acctttagcc ttacaaatgt taagggcaaa gttacgaagg
     3421 atatcatttc cgtatacaga atgacgaact tctgggtgga attggatacc gtaaatgtgt
     3481 ttatctgggt tttcgatggc tgcgtatggg cagtcagctg atgtacctat acgaacaaag
     3541 tcagcaggaa tctcagtaac cgcatcacca tggctcatca aaacagtctg ttcatcaggt
     3601 gttgattcaa aaagcgctga tggtgtgtga gttagggttg attgaccgta ttcacgattt
     3661 ccagcatcac ctgcaggaac aacttttcct ccaagtttat gggtcaataa ctgcatacca
     3721 taacagattc ccaaaattgg aattccgagt tcgaagattt ctgggtcaat atcaaatgaa
     3781 ccatcttcat atacagaatt tggaccacct gatagaataa ttcctacagg attgacttca
     3841 cgaacttcag cagctgaaat tttatggctt tttagttctg aaaaaacacc aatctcacgg
     3901 atacggcgtg aaatcagctg gttgtactgg ctaccatagt ccaatacgat gattttttct
     3961 acatcttgca aatcagttga aatgttgctc atctttttcc tttctcaaaa gaaatatctt
     4021 ttctcaatat tctatcacaa ataagggcga attaccaact aaacaaggcg aagtttgact
     4081 ttttctagtt tattggggta caatagagaa aagatagaaa tgaagtgaaa aatatgctac
     4141 ctgcttatat aaaaatccat gatcagataa aaaaggatat tgacgagtac cgttgggcta
     4201 ttggtgagag gcttcccagt gaaagagatt tagctgagca gtttgcggtc agtcgcatga
     4261 ccctccgcca agccgtatct ctattagtcg aagaaggcgt cttagagcgc cgtgtaggaa
     4321 gcggcacctt tgtttccagt actcgagtac aagaaaagat gcgagggaca accagtttta
     4381 ctgaaattgt caaatcccaa ggtaaagttc cctctagcca gctcatttcc tacaaaaaaa
     4441 ccattcccaa tgagcaagaa gttgccaagc taggaatttt tccaacggac aatattatcc
     4501 gaatggagcg ggtccgctat gccgaccaag ttcccctagt ttatgaagtt gcttctattc
     4561 ctgaaaaatt cattaaggac tttaaaaaag aagaaatcac cagtcatttc ttccaaacct
     4621 tgcaaaaaca tggctatcgt atcggcaaat ctcaacagac catctatgct cgccttgcta
     4681 aagaaaagat tgcccactat ttggaagttg aaaaaggaca tgctattctt ggattgacac
     4741 aggtttccta cctagaagat ggtactgctt ttgaatacgt aaaaagtcaa tatgtaggcg
     4801 aacgctttga attttatctt gaaaataatt agattaccac gcaaaaactc tctgtcacag
     4861 acaaagagtc ttttttattt ttctaagaat aaatctttca aagaaatcaa agttacagca
     4921 aacaatatca ttgccatacc aagaaaatca atgggataaa attgttcatt cattattaag
     4981 aaggcaaaga aaaccgccga tattggctca attgaagcca acaagcttga cttgaccggt
     5041 cctatcagac tggctccttt aaggaaagct gtataggcaa agacagtccc gataaggata
     5101 atacctgcaa acgcaaggag aaaatcaaga ctagtcggga tattggcctg tagaacccct
     5161 gtaaaaggaa gggcgaccaa acctgctata accattccca caccaatgac caagctgctc
     5221 ccccactttt taatcaaggc tatgggtaaa atgatatata gagcataagt caaggcagaa
     5281 aagagacccc agaacaggcc agcaggtgtc atggataact ggtccaactg cccatgtgtt
     5341 gcgatcagga aggttcctcc gatggcgaat atgatggaaa ctatctctcc cagtgtcggt
     5401 gccaccctat ccttgataca gctataaatt aaaattccga caggacaaac atactgaagc
     5461 accgtcgctg ttcccgcatt ggtctcctga atagcagaca gataggcgaa ttggttgaga
     5521 aaaagaccaa tcagagcaaa aataagaaga gacagcaaac tctttctatc ctttaaaaag
     5581 accagtattt tatcctttgc agtagcataa gccaagatca tgagaattcc accagcgatt
     5641 aaaagacgca agttggtcaa gaccagagcc gaaattccgt gtgccattag gtattggcca
     5701 ctcgttcctg acacccccca agcaatacca gccacaactg ttagtaaagt cccttttaaa
     5761 ctatttgaca tgtctctcct tactcttctt tacgaatcaa gttcatcact tgattgcggt
     5821 ctaatatcca ttgagctctc tcgtcctttt cataggtacg attcgatagg aaaatagtgg
     5881 cttcttgctt ctgacgattc cacatgataa aggtacctgt atagcccgta tggtctagcc
     5941 aatctccttc caaattccat gctaaagaac gttccttgtc atccaaagga gaaaaatttt
     6001 gatttaagtc tcttgcaaaa tcatctgcta aatagtgttc taaaaagatt tgtaaatcct
     6061 ttatagtcga aaataaacca gcactcccag catgtctacc caggagacga gccttgggat
     6121 catgcactat gcctgcctct acacctctaa ctgttggaac agcaagctca actggcccaa
     6181 acttggtttc cgtcattccc caaggtttcc agacttgatc ctttaaaatc acatccaaat
     6241 cttgattaaa aattctttcc aaaataaagc ccaacagcaa aaaatggaca tccgaataaa
     6301 gaaaggctgg ctgacttcgt ctgttgagat gaaacatcgc ttcctttaat tcaggggctg
     6361 ttaaaagatc acgattagga ataaaaggat caaggtctgt tgcatgagtc aagagctggc
     6421 gaatagtgat gtctggataa tcactctcag gtaaaaaatc tattaccagt ctatcaatat
     6481 ctaattgacc tatttcccac aagaaggtac aaactgtgcc aaccccaaca accttgctga
     6541 cactagctag gtcataaact agtcctgcct cagtctgcaa gccatgctct gggtcactct
     6601 ggcctaaata gaactctgtc cattgattgt ccttaaaata cgcaaaagag gctccgggat
     6661 aaattcctgc ctcgatttgt tcttctattt ttttaataat cttggtccac ttcatacttc
     6721 ttcaaaccac aattcaacat tatttctatc tttacaaagg aagaattttt cagacttagg
     6781 aataaaatat tcagtagact caaacttctg gcgaagactt gctatgtcta attcattgac
     6841 caagaacttg agcatagata agtcccaggt aaccgtattg tccacagtca aatcctgccc
     6901 ctgagctgga ataaaatcaa gggatgcccc aatttcagat gattccaaga aactttcgat
     6961 atcagttggg agatgtaatt ccatagaaat ctcaaattta cttaaagaaa ttgatgccaa
     7021 atctgtttga aattcaggct tttctcctac ttctactaga cttgctatgt catcttccgc
     7081 atgaatcaaa atcaaatcat cttctggtga gaaaatttca aaagcgtagc cattttgacc
     7141 tttatataat cgatgaatcg aatctgtttt agataagatt ccttcaattt ctaagggatt
     7201 ttccaccttg acaatcaatc tagctagttt ttttcttccc tctaccttac gagtacgcat
     7261 actgggagct tcttctaaaa ccagcttttc aagacccgtt tggtcaccta gtgacagaaa
     7321 ggccgattct tctaacaagg ccttcattcc aagggtttca atataaaatg tttcatttaa
     7381 ttttctatta ttagctttta aagtaggaat aatccgtaca atctgattta cattcataaa
     7441 ttcctccgtc actttttatt ttaaaggatt ttagaatttt ttacaagata ttatgctcaa
     7501 atttaacatt atttagctat ttagtaaaga tagtctttca aggattttga caaagcctga
     7561 taaccagcaa tactgaggtg cagtccatca gttgtatatt cttttttgag ttggcctgct
     7621 tggtctgtca aacaatcaaa tactggcaca aattccacct gcatataggc agatgcaagc
     7681 tcttgatagg cttgattcca attctgaatt ttttcattcg agcggatata gactgcctgc
     7741 tggtacttct ctccctcatt gacaggcaaa atggaaagca atttaatctc tgtcaatgga
     7801 taatcgcgag caacggattg aatgatagct tcgagattat tgagaacctc attcacagga
     7861 acatcctttc cgatatcatt tgtcccaatc agaagaaaaa ttttatctac tgctccacca
     7921 tatagatgag catcaaggtt ctctagtaac agtcctgtct gatagccacg aattcctcga
     7981 ttgacaatcg tctttgaagt cccaaatagc tcctgtagag gataatactc gacaatggaa
     8041 tccccaataa aaagaatgtt tggttctaca acagaaatgt gatttaggtg acgatatttg
     8101 gtttgaattt tttcttgttc ctttaggagc caattttcta ataactgtac taccacttca
     8161 ttctcctttt tctaaccagt tttccaagac ttgataaact cctccttggc tgttggctgg
     8221 tgctagagca gtcgccacag ctttggcttt ctcatcagca ttttccatcg cataggcaat
     8281 tccagccatt tcaagcattt caacatcatt ttcactatca ccaaaagcca tgatttcttg
     8341 ggatttcaag tcccagcgct tgagtaattc ctccaagccc catgctttat gaatcccagc
     8401 ttggaggata tcaatgcaac catagccact ggatacagct cggacacggc catcaaagag
     8461 agcattgatt tcttccaaaa ccgaactcaa acgttcctca ccaacaacca tgctcatctt
     8521 gagcacacca ccaaagaggt cagatgttaa ttcatccaca aattgcatcc gttggtagaa
     8581 tttttcaatc atttctggag tcataaaact ttcaagatct gtaaaaatcg taccttcctt
     8641 gacaaaatca cccttcatcc ccgttacaac aaactggttc tgacacgctc gacccttgaa
     8701 atgagccaaa gccttgttga caatggcgtc atcccatgtc tgagcctgaa tcaattcatt
     8761 gttttcaaaa atacgagcgc cattagcaac aaccagaacc actcgatcca ccaagggact
     8821 cagtagttgt ctcatgcggt gaatttcatt gcccgtcgca atgacaaaac gaatgcccct
     8881 ttgatccaac tgatctaaaa tcttttccaa tcgtgggaga tcaagctgac ctctagcatc
     8941 cagcaaggtc ccatccatat ctgtcgcaat taccttaatc atcatttact ttcctctgga
     9001 ttcaagctaa taccacttca atcccacatg ttcattcatt tctttatagg ctgtatcaat
     9061 tcgttcctta gtcgcttcat ctaactggtc acgatgactg ccacccctct ataaaatctt
     9121 ccaaaatata agtttggtaa tgatacaagg gatatccttc tggtgaaaag gttccctttg
     9181 gtgttcgatt gacctaagta ggatgagctt tagctgttcc gatagttgtt tttccatcct
     9241 tcttcttgat ggtgacatcc atgagaacac cacgttcagt ccacttagca ttctcttcat
     9301 ctcccataga ttcaatccgt tgattggaaa tgaagttccc catttaatag acaatgagct
     9361 tcttatctcc atctttttcc accgtttcag atggttcaac aacgtgagga tgccctccaa
     9421 agataatatc cgctccccaa tcgatcatct tgtgataaag agctttttgt tcttcagttg
     9481 gttccaatcg atactcaaca cccatcagag gcatgataat ggtgatatct gcttccttct
     9541 ctgcccgttc aacttcagcc ttcatcttat cttcgtttaa atctaaaaga taacgattat
     9601 agtcttcctg agaaatatac tgctcaattc cattgaaacc ataggaatag gctaacaatg
     9661 caaccttgat accattcact tccttaatga ccagcggagc ctgaacacgt ggttcgtgcg
     9721 tataaactcc gattggagtg attccagctt tctcaataat atcggccgtt gaaataactc
     9781 cctcaatttg cgaatccaaa atatgattat gagctaaatc cagcacatga taacctgcct
     9841 ccttaatagc atccataact tcagcaggag cattaaagag aagataaccc gctaaataat
     9901 gatccttatt aatggttcct tcaaaatcac caatagctaa atctgcttgc ttgagccaag
     9961 gagtcacata ctcaaaattt tcatgaaagt cataggtacc gtcttctttt ttagctgaaa
    10021 agaaaagtcc atcgtggtag agcaaatccc cgtgggccat aattctggca gttttttcct
    10081 cttcctactc ctgagacctt tgcttagtcc cctcttgaga aatagtatct cgtttttgac
    10141 tagtcaaagg attccttgga agctttcaaa caacaagacc aagcccattg ataaaccaac
    10201 tgctagcaag agtatcgcca caaatccctt attgctccac ttgcgataac tcctaaaaag
    10261 tttaccaagc ccttcataaa acgaaaagct aaaccaccct gatttcgatt ttgtcttctt
    10321 tgtatcttca ttctccctac tttcttatgc aagccttttc tttttattat atcacagata
    10381 agtatttctt tcacaattga attgaacttc ccatctattt tctataaatc ctaaatgcca
    10441 taatgctttc aattcttgtc attttgtgat atcatgtaga ggaaatgaac taatccacag
    10501 tggcttattc caagtatacc acttgggctt tggcagtagc taactgcact aaacataata
    10561 taaggagaga aaatgtctgc aatagaacgt attacaaaag ctgctcactt aattgatatg
    10621 aacgatatta tccgtgaagg gaatcctact ctacgcacgg ttgctgagga agtcactttc
    10681 cccctatctg accaggaaat catcctaggc gaaaagatga tgcaattcct taaacattcc
    10741 caagatcctg tcatggctga aaaaatggga ctccgcggtg gtgttggact ggctgctccc
    10801 cagttagata tctcaaaacg cattatcgct gttttggtac ctaatattgt tgaagaaggc
    10861 gaaactccac aggaagccta cgatttggaa gccattatgt acaatccaaa aatcgtctct
    10921 cactctgttc aagatgctgc tcttggcgaa ggagaaggtt gcctgtctgt tgaccgtaac
    10981 gtgcctggct atgttgttcg ccatgcccgc gttactgttg actactttga caaagatgga
    11041 gaaaaacacc gtatcaaact caaaggctac aactccattg ttgttcagca tgaaattgac
    11101 cacattaacg gtatcatgtt ttacgatcgc atcaatgaaa aagacccatt tgcagttaaa
    11161 gatggtttac tgattcttga ataaagaaaa tcccgttgca agacggggtt ttgtgttata
    11221 atagaggcat gaaaacaaat gatattgtct atggtgtcca cgccgttacc gaagccctcc
    11281 ttgcaaatac aggaaacaaa ctctacctcc aagaagatct ccgaggtaag aatgttgaga
    11341 aagtcaagga actagctaca gaaaagaagg tgtccatttc ttggacatca aaaaaatctc
    11401 tctctgagat gactgaaggt gctgttcatc aaggttttgt tctacgagtg tctgaatttg
    11461 cctatagcga gctagattac atccttgcaa aaacacgcca agaagaaaat ccacttctat
    11521 tgattctaga tggtctaacc gatccccata atctaggttc tatcttgcga acagccgatg
    11581 cgaccaatgt ttcaggtgtc atcattccca agcaccgtgc tgtcggagta actcctgtcg
    11641 ttgccaaaac agccacaggt gctattgaac acgttccaat tgcccgagtg accaacctca
    11701 gtcaaacctt agataaactt aaggatgaag gtttctggac ctttggaacg gatatgaacg
    11761 gtactccttg ccacaagtgg aatacaaaag ggaaaatcgc cctcatcatt ggaaatgaag
    11821 gaaaaggtat ctctagcaac atcaaaaaac aagtcgatga gatgattacc attccgatga
    11881 atggacatgt tcaaagcctt aatgccagtg ttgctgcggc cattctcatg tacgaagttt
    11941 tccgaaatag actataaaaa gtttccagtc atctgattgg aaactttttt ataattaact
    12001 atgttctgta atgaatttat aggcttcttg accagcgata gctccatctc caaccgctgt
    12061 tgttacttgg cgaaggtctt tcaagcgaac atctccaact gcaaagatac cgtcgactgc
    12121 agttttcatg tggttatctg tcacaatcca tcctgcctga tcttggatat tcaattcttt
    12181 aacaaaatcg ctaagagggt ccaaaccaac atagataaag acaccaccga agacttgttc
    12241 tgtcacttga cctgttttca cattttcaaa tacgactgat tctactcggt tttcaccctt
    12301 gatttccctt actacagaat cccagataaa actgattttt tcattcgcaa aggcgcgatc
    12361 ttgtaaaacc ttttgggcac gaagttggtc acgacggtga acaatggtaa cagtcttagc
    12421 aaaacgagtc aagaagaggg cttcttcaac agctgaatct ccaccaccaa ctaccaataa
    12481 atcttggtca cggaagaaag caccatcaca cacagcacag taagaaacac cacgactgtt
    12541 cagttcttct tctccaggta ctcccaaagg acggtgttta gaaccagttg ctacgataac
    12601 tgtacgtgtt tcatatgttt ggtcatcagt catcactttc ttaaaatcac catggtcttc
    12661 gacattttca acataaccat aaatgtgctc cacaccaaga ttttcaagtg gttcaaacat
    12721 cttttcagcc aattcaggtc cactaatatt agcgtatcct gggtaatttt cgatatcaga
    12781 tgtattattc atctgaccac ctggcagacc accttcaatc aaagctactt ttagattgct
    12841 tcgagcagca tacaaggccg cagtcatccc tgcaggtcca gcaccgataa taatagtatc
    12901 gtacatatag attccttctt tcttgttgta actatcttta ttctaactct ttcttgtcaa
    12961 tcacgcaagg attatgcctt gaaaacaaga attaaactca taaccgcaaa ggataatact
    13021 gggacaacca agactccaat cataatcata tcatagagat gggcttgacg agccctggct
    13081 gtcttatcaa ctcccgacat ggctctcaga ccaatccaaa tccctaaaaa aatcagaaca
    13141 aggatggtgg tcaagatcaa actctcgaaa tataaagaaa atagttgcag tagcatgatt
    13201 tctctcattt ctatcttttt taaagagtaa actcagctag tccaactaac tgagttttcc
    13261 tttatctatt atatcaaata taagtccgtt tgtaactagc gaagaattct tttgtccgct
    13321 cttctttagg ggtgtggata atctcatccg gagttccaga ctcgatgatt ttccccttat
    13381 ctaagaagag aattttatcc gcaacttggg ctacaaagga catgtcatga ctgaccaaaa
    13441 tcatggtctg acctgactta gcagcatctg caatagactt ttctacttca ccgaccaatt
    13501 ctgggtcaag ggctgaagtt ggttcgtcta agagcaaaac atctggtttc atagcaagcg
    13561 cacgcgctag ggcaacccgt tgcttctgtc cacctgataa atggcgagga taatggtttt
    13621 cacggtccga aagcccaacc ttagccaact cttccttggc aatcttagtc gcttcttggt
    13681 cagataattt cttgacaaca accaagcctt ctttcacatt atcaagtgct gttcggcgtt
    13741 caaacaaatt aaactgttgg aaaaccatag acaacttacg gcgtagggca aggatttctt
    13801 cttgagtgat tttagaaaaa tcaactgaaa aaccatcaat ctgaatagag ccactgtcag
    13861 gtgtttcaag ataattgaga ctgcgaagaa aggttgattt tccagctcct gaagaaccaa
    13921 tcaaggctac gacttctcct ttttgaatat ccaagttcag atgatccaag acagtctgtc
    13981 ctgaaaagga tttgcttaaa ttcgaaatct taatcattaa cgaaggtctc ctttcacatc
    14041 tgtttgcact gtatcaggtg cagaaatagc catttttctc tcgatgaaac gaccgaggct
    14101 ttcaattccg atattgacta cccaataaac aagggcaacg gagatgaagc gttcaaaata
    14161 gcgataatca gctccaccta gaatctgagc ttgggcaaag acttccacaa cacccgcact
    14221 aaaagctaga gatgttccct tggtcaaacc gatgagggaa ttaatcaagg ttggagtagc
    14281 taccaccgct gcattaggaa taatcactcg tcgataaact tgcgctcggg tcatacccag
    14341 actgcgtgcc gcctcaatct caccaggatt aactgagaga atggctgcac gaatggtttc
    14401 actagcataa gctgcctcat taaaggcaaa ggcgacaatc gcaaaagctg cagctggaat
    14461 cgcattgata ttgagaccag ttccccattg ctgattgagg gctttcaaag ccaaaggaat
    14521 tccgtagtag gtcaacatga gttgcaccaa aatcggtgtc ccttttaaga aactaacaaa
    14581 gaaggcctgc aagggatata aaatcttgac acgattgatc ttcacaatgg caaaaagaag
    14641 cgccaaaacc aagccaaaaa gggcaccgcc aattgtcaac ataattgttg ttggaagttg
    14701 ttggacaatt ctagggattc catcaaagac cgaacgtagg ctaaacagct tgccatccgg
    14761 aatcaattgc atcaagtttt ggtaccaatc tgatgctaaa atcgttgtaa cattcataaa
    14821 aacatcctct tctgataatg attcattcta ccatgcttct gccgtcaatg aaattatttg
    14881 ctacggtcag atacagactt tgtctaccta ctagtttatc atactttgaa acagggaagt
    14941 tatatatttt atctatcaaa gagatagata aaaactattt cagtcccagt tcttcataag
    15001 attttcgata accgatttgc ttaacagttc cattttctac taaaatgggc cgttttaaca
    15061 acataccgtc acttgctagc aactcagccg cttcttggtt tgacaaactt cctaccttat
    15121 cttttagccc taattcacgg tatttgatac cactggtgtt gaaaaattgc ttcaattcaa
    15181 atcctgaggt ttctagccaa ttcaaaatga cttcttggct aggtgtttct tccacgatat
    15241 ggacggcttt atagtccaca cctaattgat ttaattcttg ttttgctttt ttacaagttg
    15301 aacatttggg gtattcgata aattctaaca tgtgcttctc cttctattgt ttctatcttt
    15361 aaaatctact ccttcatgct ccaagagcca agctttcttt tccactcctg cagcataacc
    15421 tgtcagacgc ttgcctgctc ccaacacacg atgacaaggt actaggatag accaaggatt
    15481 gcgtcccact gctccaccaa ttgcttgagc agaagccact tgcaggtctt gagcaatttg
    15541 tccataggtc actgtctgac cataaggaat gccctgtaaa tagtcccaaa ctctcttttc
    15601 aaaattcgtt ccgattggcg ccaagagcaa gttggataaa tcctgaggct tgcctttaaa
    15661 gtaatcatct aagcaagcaa taactgggtc taaaatagga tgactaacaa cttcttctat
    15721 cgtttcatct cctagtcccc tctcaaaatg cttctgctcc tgaacccaaa ttccatacaa
    15781 ataatggtca tcagctacaa gtgatagaat accaattggt gaggagtaga ggatttttac
    15841 atacttcttt tgcattattt ctttaatttt tgataggcca agcgttcgcc atctactggc
    15901 accttaccaa cttgtttaaa accaagtttt tcaaaaatat gttgcataac cttgttttca
    15961 gcatgcgtat ctgagcgaaa atcaagataa tcaaaacctt caatcaagcc ctctaagaag
    16021 gtttgagcaa ctccttttcc ctgcacatct gctgccacag caatacggtg aaagactaga
    16081 tactctgact ctcccgcttg ccagtttccc tcataaatag cttcataggc ctcctctgga
    16141 ctcttggtca cagcagcata ggctagcagt tctccctctt ccaaggctac gtaggcttgc
    16201 cctgagatga tatcatcgat aataacatct gcatttggat agccattttg ccactggtca
    16261 ctaccagcat ctgctaaaca ttttttggca tcctccatca cctgcatgat ggcatctact
    16321 tcatttggaa aagctaaacg aatttccatc ttttttcctc atttctgact agtttctccc
    16381 atcatagcat aatttaagta tttctacaag cagttaaaac ttgactttgt gtttttgtta
    16441 ttttataatc tagttatcaa aactatataa aaaggagttg aaaatgaaaa ctaccttttc
    16501 ctacccaaaa tgggcagaaa ttccaaacat tgacctctat ctggaccagg ttttgctcta
    16561 tgtcaatcag gtctgcgccc ctatctctcc taataaagac aagggcctaa cagcatctat
    16621 ggtcaacaat tatgtgaaaa atggttacct gacaaagcct gacaaaaaaa aataccaacg
    16681 ccaacagatt gcccgtttga ttgctatcac aaccctcaag tctgtatttt ctattcaaga
    16741 aatagcccag acacttaata ctctacaaac tcaagcaagt tcagaccaac tctacgatgc
    16801 ttttgtggac tacatgaacc aaggaattga cccagctaac cctattatcc aaaccagctg
    16861 ccaaaccgtt aaactctatc atcaaactct agacttaatc gatcatactc aagaggaggt
    16921 aatccaatga acactagtct taaactcagc aaacaactca gttttggaga ggagattgct
    16981 aatagcgtga cccatgctgt gggtgcagtc atcatgctta tcttgctgcc tatttcatcc
    17041 atctatagtt atgaagcaca cggattttta tcctctatcg gcgtttccat tttcgtcatc
    17101 agtctctttc tcatgttcct atcatccacc atttatcact ctatggccta tggttcgacc
    17161 cacaaatatg ttttgcgaat cattgaccat tctatgattt acgttgccat tgccggctca
    17221 tacacgcccg ttgtcttgac cttgatgaat aactggtttg gctatctgat tattgtcatc
    17281 caatggggaa cgaccatctt tggtattctc tataaaatct ttgctaaaaa ggtcaatgag
    17341 aaatttagcc ttgctcttta cctgattatg ggctggttgg ttctggctat cattcctgcc
    17401 attatcagtc aaacgacacc cgttttctgg agtctcatgg taactggcgg actctgttat
    17461 acagttggag ctggatttta tgccaagaaa aaaccttatt tccacatgat ttggcatctc
    17521 tttatcctag ctgcgtccgc actccaatac atcgctattg tttattacat gtaaaactct
    17581 aagcaggcag agcatgctta gagttttaca atcgaatttc ccaaagagga agcaagatga
    17641 aagccatcat tgcttgcgca cataggattc tcagaatcat ttacaaacta ctctccacca
    17701 agcagactga tcaaacagaa aaggcgctag aactgaggaa acagttctaa cgcccaaatt
    17761 aaaaaaattt caattacact atagcacagg aagtgatttt ttgcgctttt ttactgtctt
    17821 ttttcaaata aaaaagtatc gctttggttt caatgtcaat aagtgtgttc tttttgaatc
    17881 agtaactatt ccaatttatc tatcttgaaa ta
//
LOCUS       DAVLVP010000033        17705 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.033, whole genome shotgun sequence.
ACCESSION   DAVLVP010000033 DAVLVP010000000
VERSION     DAVLVP010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17705)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17705)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17705
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.033"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            106..366
                     /locus_tag="VYN69_001078"
     CDS             106..366
                     /locus_tag="VYN69_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533364.1"
                     /translation="MRERSATGAQGLSKSIKKHLNDLTRLTASLLGDEKLSAITSSSA
                     VKADMHRFVIELEPVKSTILQNNDISLDQNEIFEILKNFLDG"
     gene            complement(1104..1906)
                     /locus_tag="VYN69_001079"
     CDS             complement(join(1104..1456,1456..1906))
                     /locus_tag="VYN69_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HEV1533365.1"
                     /translation="MNYEASKQLTDARFKRLVGVQRTTFEEMLAVLKTAYQLKHAKGG
                     RKPKLNLEDLLMATLQYVREYRTYEQIAADFGIHESNLIRRSQWVEVTLVQSGVTISR
                     TPLSSEDTVMIDATEVQINRPKKELANYSGKKKCHAMKAQAIVTSRGRFVSLDITVNY
                     CHDMKLFKMSRRNIGQAGKILADSGYQGLMKIYPQAQTPRKSSKLKPLTAEDKAYNHA
                     LSKERSKVENIFAKVETFKMFSTTYRNHRKRFGLRMNLIAGIINHELGF"
     gene            2031..2489
                     /locus_tag="VYN69_001080"
     CDS             2031..2489
                     /locus_tag="VYN69_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000186958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEV1533366.1"
                     /translation="MTPEEMYLTERLDVQIAHFLKKSVQHRRRYKVLKITEIVAGFLI
                     AVFCAIPMPGDRYRLISVALSSLGLLCEGIINLYNAKENWISYQKTAQLLEKEKFLYQ
                     CQTEKYAGKTKAFALFVKTCEGLISEEINQWESIQSKEVAASADAPVKKE"
     gene            2502..3110
                     /locus_tag="VYN69_001081"
     CDS             2502..3110
                     /locus_tag="VYN69_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533367.1"
                     /translation="MSQSSYLSPLLWLKKEADKEKMSATQCQIFFFYYQMFELLFARE
                     SDMKDLCLGTKGFYFSQLEKNLLSGVSRFLKNLEGKVTLKANQEVSARKALFLALTTS
                     QSDWQELAPVFDFYQTIGRLENPSLLSSQDRQHLMWIYQSALEKDYIVKVIGDKHFVL
                     KRQDATKLTARQTQTLEILSQSEDLVNPVYVTLGEKGVLLLD"
     gene            3181..3513
                     /locus_tag="VYN69_001082"
     CDS             3181..3513
                     /locus_tag="VYN69_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533368.1"
                     /translation="MYICIDNSMTKNKELVKNQTFKPALLTRRLVKNFMIEEPDLARN
                     PFTRPTLIDLDKVFMLDNTVIPTSYLARRRRNVSEELYEEILDHLVQPRLISLNKSEF
                     MQLNPGTY"
     gene            3524..4939
                     /gene="ply"
                     /locus_tag="VYN69_001083"
     CDS             3524..4939
                     /gene="ply"
                     /locus_tag="VYN69_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cholesterol-dependent cytolysin pneumolysin"
                     /protein_id="HEV1533369.1"
                     /translation="MANKAVNDFILAMNYDKKKLLTHQGESIENRFIKEGNQLPDEFV
                     VIERKKRSLSTNTSDISVTATNDSRLYPGALLVVDETLLENNPTLLAVDRAPMTYSID
                     LPGLASSDSFLQVEDPSNSSVRGAVNDLLAKWHQDYGQVNNVPARMQYEKITAHSMEQ
                     LKVKFGSDFEKTGNSLDIDFNSVHSGEKQIQIVNFKQIYYTVSVDAVKNPGDVFQDTV
                     TVEDLKQRGISAERPLVYISSVAYGRQVYLKLETTSKSDEVEAAFEALIKGVKVAPQT
                     EWKQILDNTEVKAVILGGDPSSGARVVTGKVDMVEDLIQEGSRFTADHPGLPISYTTS
                     FLRDNVVATFQNSTDYVETKVTAYRNGDLLLDHSGAYVAQYYITWNELSYDHQGKEVL
                     TPKAWDRNGQDLTAHFTTSIPLKGNVRNLSVKIRECTGLAWEWWRTVYEKTDLPLVRK
                     RTISIWGTTLYPQVEDKVEND"
     gene            5822..6538
                     /locus_tag="VYN69_001084"
     CDS             5822..6538
                     /locus_tag="VYN69_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015604432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1533370.1"
                     /translation="MGRKWANIVAKKTAKDGANSKVYAKFGVEIYVAAKKGDPDPESN
                     SALKFVIDRAKQAQVPKHIIDKAIDKARGNTDETFTEGRYEGFGPNGSMLIVDTLTSN
                     VNRTAANVRAAFGKNGGNMGASGSVSYLFDNKGVIVFGGEDADAVFEQLLEADVDVDD
                     VEAQEGTITVYTAPTDLHKAIVALRESGIEEFQVTELEMIPQSEVELSGEDLETFEKL
                     YSVLEDDEDVQKIYTNVDGF"
     gene            6662..7111
                     /locus_tag="VYN69_001085"
     CDS             6662..7111
                     /locus_tag="VYN69_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEV1533371.1"
                     /translation="MGKPMLVFKRFGHQIHLMVQKEAKRCGIEFMGGPQGQVVRFLDN
                     REKNQDLVLIKDIEQELNITKSVASNLVKRIVQNSLVELEASPVDKRAKFVRLMDKAR
                     SQMQQVKAFFERIDKQLMEDIDEDELLIFEKVLGQLQAKYQGNRRRE"
     gene            7181..7594
                     /locus_tag="VYN69_001086"
     CDS             7181..7594
                     /locus_tag="VYN69_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533372.1"
                     /translation="MLFTCFSVYLELEVPTYISKITDLLGSQGTNLDELWQPASMMMG
                     MPFLAFLSVVAVGFFASRVAASYTSRLRSDIFNRVLDCSQTEIKKFSIPSLLTRTTND
                     ITQVQMLITMGLQVVTRGPIMAIWAIGKILGHSEY"
     gene            7634..7976
                     /locus_tag="VYN69_001087"
                     /pseudo
     CDS             7634..7976
                     /locus_tag="VYN69_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214321.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            7983..9014
                     /locus_tag="VYN69_001088"
     CDS             7983..9014
                     /locus_tag="VYN69_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000506433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1533373.1"
                     /translation="MGFLLMGALFIVLPRTMVSAKRINQVLDLHSSIQNPVQVQLTDE
                     NFKGQVEFKDVTFRYAANSEAVIEHVSFKAETGQTVAFIGSTGSGKSTLVNLIPRFYD
                     VSAGEILVDGVNVQDYDFSATAHAGQKVAIVGPTGTGKTTIVNLLMKFYEIDKGSIHI
                     DGVDTKAMTRSEVHDAFSMVLQDTWLFEGTIRDNLIYNQIGISDERMMEASKAVGIHH
                     FIMTLPDGYDTILDDTVTLSVGQKQLLTIARALLKDAPLLILDEATSSVDTRTEELIQ
                     KAMDRLMEGRTSFVIAHRLSTIRNADLILVMKDGNIIEQGNYEELMAQGGFYADLYNS
                     QFTEDEAEE"
     gene            9080..9442
                     /locus_tag="VYN69_001089"
     CDS             9080..9442
                     /locus_tag="VYN69_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEV1533374.1"
                     /translation="MSQKLYNMKFAAVYLALIAKVERKGGKAESVHQVTSWLTGYEVS
                     DVLACLDRDVTYGDFFRQAPYYVPERIAITGKICGVRIEEIDDPLMQEIRRLDKLVDW
                     LAKGKTSQQVLEKYEKHK"
     gene            9439..9942
                     /locus_tag="VYN69_001090"
     CDS             9439..9942
                     /locus_tag="VYN69_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1533375.1"
                     /translation="MKNYQEWYRNISSRLTSRPILLFLLRSFNRLMTVAMPLVYLILL
                     VTTYLQLGLGQQVGVYVLIPASGFVALSLFRKKINHPRPYETWDICPLLDKDSSGRSM
                     PSRHVFSATIISMACLHASLPIGLVCLIFSALLGLVRVLGGVHYPKDVLVGYACGLVW
                     GFLFFLF"
     gene            10056..10502
                     /locus_tag="VYN69_001091"
     CDS             10056..10502
                     /locus_tag="VYN69_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1533376.1"
                     /translation="MKLRIEIDGNLEETEIVIKTPTLTDEIADLQRLLQESKAPRLTF
                     YKGTGEYYLDLSEILFFETEGSKIYAHNQKEAYEVRLKLYELESILPRYFNRVSKSTI
                     ANIRQIYSVDKSFSGTGTISFYQTHKEVHVSRHYQSLLKENLRNMR"
     gene            10508..11182
                     /locus_tag="VYN69_001092"
     CDS             10508..11182
                     /locus_tag="VYN69_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000725744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533377.1"
                     /translation="MKKKAFGIVLLVLAAWILLQGNFGIPSLDGKIWPLLGIVFFAYK
                     SIESILRRHLTSAVFTGLLALIIANYAYDLLPVTNHSLIWASILVVLGVGYLTHSSKF
                     WNEKKWWYNGKKTVVTDKEVAFGSGTFYKQDQDLVDDQVEVAFGDAKIYYDNAEMLGD
                     FATLNIEVAFGNATVYVPQHWRVDLKVETSFGAAKADAPVAPTSKTLIIRGDVAFGKL
                     EIVYVK"
     gene            11437..11625
                     /locus_tag="VYN69_001093"
                     /pseudo
     CDS             11437..11625
                     /locus_tag="VYN69_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647592.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein A"
     gene            11875..12174
                     /locus_tag="VYN69_001094"
     CDS             11875..12174
                     /locus_tag="VYN69_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4651 domain-containing protein"
                     /protein_id="HEV1533378.1"
                     /translation="MNGMKAKKMWMAGLALLGIGSLALATKKVADDHKLMKTQEELTA
                     IVRDHFSDMGEIATLYVQVYESSLESLVGGVIFEDGRHYTFVYENEDLVYEEEVL"
     gene            12171..12488
                     /locus_tag="VYN69_001095"
     CDS             12171..12488
                     /locus_tag="VYN69_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEV1533379.1"
                     /translation="MIQPASLEELASLVEKAGKKVFLFVADWCGDCRYIYPALPEIEE
                     TNPEFTFIRMDRDQYMDLAKLWDVYGIPSLVVLEKDKEIGRFVNRDRKSKEQINDFLA
                     GLK"
     gene            12504..13130
                     /locus_tag="VYN69_001096"
     CDS             12504..13130
                     /locus_tag="VYN69_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 and tRNA-binding domain-containing
                     protein"
                     /protein_id="HEV1533380.1"
                     /translation="MIFTYNKEHVGDVLMVIVKNSGDAKLNVERKGKVARVFLKENGE
                     TVAWNIFEVSSLFEIAERGQVFLSDEQVARLNQELQAEGFTEEIVNDKEPKFVVGEIV
                     EMVAHPDSDHLNICQVAVASDKIVQIVAGAPNARVGLKTIVALPGAMMPKGNFIFPGE
                     LRGEKSFGMMCSPRELHLPNAPQKRGVLELSEDQVVGTPFDPAKHWTA"
     gene            13163..13924
                     /locus_tag="VYN69_001097"
     CDS             13163..13924
                     /locus_tag="VYN69_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HEV1533381.1"
                     /translation="MAKNVVITGATSGIGEAIARAYLEQGEDVVLTGRRIDRLEILKS
                     EFAVSFPNQTVWTFLLDVTDMTMVKTVCSDILETIGQIDILVNNAGLALGLAPYQDYE
                     ELDMLTMLDTNVKGLMAVTRCFLPAMVKANQGHIINMGSTAGIYAYAGAAVYSATKAA
                     VKTFSDGLRIDTIATDIKVTTIQPGIVETDFSTVRFHGDKERAASVYQGIEALQAQDI
                     ADTVVYVTSQPRRVQITDMTIMANQQATGFMIHKK"
     gene            14002..14397
                     /locus_tag="VYN69_001098"
     CDS             14002..14397
                     /locus_tag="VYN69_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEV1533382.1"
                     /translation="MYNKVILIGRLTSTPELHKTNNDKSVARATIAVNRRYKDQNGER
                     EADFVNMVLWGRLAETLASYATKGSLISVDGELRTRRFEKNGQMNYVTEVLVTGFQLL
                     ESRAQRAMRENNAGQDLADLVLEEEELPF"
     gene            14553..14837
                     /gene="groES"
                     /locus_tag="VYN69_001099"
     CDS             14553..14837
                     /gene="groES"
                     /locus_tag="VYN69_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEV1533383.1"
                     /translation="MLKPLGDRVVLKIEEKEQTVGGFVLAGSAQEKTKTAQVVATGQG
                     VRTLNGDLVAPSVKTGDRVLVEAHAGLDVKDGDEKYIIVGEANILAIIEE"
     gene            14853..16475
                     /gene="groL"
                     /locus_tag="VYN69_001100"
     CDS             14853..16475
                     /gene="groL"
                     /locus_tag="VYN69_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018166362.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEV1533384.1"
                     /translation="MSKEIKFSSDARSAMVRGVDILADTVKVTLGPKGRNVVLEKSFG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     IKNVTAGANPIGIRRGIETAVAAAVEALKNNAIPVANKEAIAQVAAVSSRSEKVGEYI
                     SEAMEKVGKDGVITIEESRGMETELEVVEGMQFDRGYLSQYMVTDSEKMVADLENPYI
                     LITDKKISNIQEILPLLESILQSNRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITEDLGLELKDATIEALGQAARVTVDKDSTVIVEGA
                     GNPEAISHRVAVIKSQIETTTSEFDREKLQERLAKLSGGVAVIKVGAATETELKEMKL
                     RIEDALNATRAAVEEGIVAGGGTALANVIPAVATLELTGDEATGRNIVLRALEEPVRQ
                     IAHNAGFEGSIVIDRLKNAEVGTGFNAATGEWVNMIEEGIIDPVKVSRSALQNAASVA
                     SLILTTEAVVANKPEPVAPAPAMDPSMMGGMM"
     gene            16613..17368
                     /locus_tag="VYN69_001101"
                     /pseudo
     CDS             16613..17368
                     /locus_tag="VYN69_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000567550.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
BASE COUNT         5290 a         3228 c         3989 g         5198 t
ORIGIN      
        1 tacaacctca aaacagtgtt ttgagcagcc tgcagctagc ttcctagttt gctctttgat
       61 tttcattgag tattaattat ttttaaggct aaagcttggc tggatatgag ggagcgctct
      121 gccacaggtg ctcaaggttt aagtaagtcc attaaaaagc atttgaatga ccttacccgt
      181 ttgacagctt ccttgctagg agatgaaaag ttatcggcta taacatcaag tagtgcggta
      241 aaagcagaca tgcaccgctt tgtgatagaa ttagagcctg tgaagtcaac tattcttcaa
      301 aataatgaca tttcattgga tcaaaatgaa atttttgaaa ttctgaaaaa ttttctcgat
      361 ggttaaaata attgtagcga gatggctata ttgaattcgt ctatatctgg aaactagaaa
      421 aaacttcaat ttcaggagaa aatgaagtca atcttcccac aatcaaacgt atagtatcaa
      481 ggtttttcaa gacctgatat tatgcgtttt ttgcttttca aaactttttg cccagtcttc
      541 gtttttatcc tctagtcact tgatttgttt caggtggttt tttagtatag tagaatgaaa
      601 cgagaacagg acaaattgat caggacagtc aaatcgattt ctaacaatgt tttagaagca
      661 gaagtgtact attctagttt caatctacta tagttaaatc tgcggtcaag tctactggtg
      721 aatctatgat tgtaatactc ttccaaaatc tcatcaacca cgtcagtctt gccttgcagt
      781 ctgtatctta ctgaccaagc tagtgatgga tttagaatag gtgatttgga gcgtcctatt
      841 ggctaggaaa tgctgctcat agtcctttgc tgaggctagg gtgtttcaac attcaacact
      901 caactggttg atctagttga taggaaggga gttactataa aatactcagg cttccatcat
      961 attttttgaa acgattgtgt aatcaaaatg taccaatatt gtagtattgg tacagaagat
     1021 gttgtgaatg gataaatata tcataactgc tatctcaaaa agatttcata tgtctgtgca
     1081 tatataatag acttcctgca aaactagaat cctagttcat gattgataat accagcaatc
     1141 aaattcattc gtaatccaaa gcgtttacga tgatttcgat aggttgttga aaacatttta
     1201 aacgtttcta ctttggcaaa gatgttctca accttgcttc tctccttaga tagcgcatgg
     1261 ttataggctt tatcttcagc tgttagcggc ttgagtttgc tggatttacg tggagtttgt
     1321 gcttgaggat atatcttcat gagcccttga taaccactgt cagccaagat tttaccagct
     1381 tgtccgatat ttctgcgact cattttgaac aacttcatat catgacaata gttcacagtg
     1441 atatccaaag aaacaatctc cctcgacttg tgacaatcgc ttgagccttc atagcgtggc
     1501 atttcttttt accagaataa ttcgctaatt cttttttagg gcgattgatt tgtacttccg
     1561 tcgcatcaat cattaccgtg tcctcagaac tgagaggagt tcttgaaatc gtaacaccac
     1621 tttgaacaag agttacttca acccattggc tccgacggat taagttgctt tcgtgaatac
     1681 caaaatcagc cgcaatttgt tcataagttc gatattctcg cacatattga agagtggcca
     1741 taagaaggtc ttctaggttt aatttaggtt ttcgtccacc ttttgcgtgt ttaagttgat
     1801 aagctgtttt taatacagct aacatctctt caaaagtggt acgctgaaca ccaacaagac
     1861 gcttaaatcg tgcatcagtt agttgtttac ttgcttcata attcatagaa ctactatacc
     1921 atattttgtt tcgcaggaag tctaataagt tatagtccag tttttactat actttgatat
     1981 tcaaatatgt tatactatgg ttatcaaagt aaatgaaagg agttacgaat atgacgccag
     2041 aagaaatgta cctgacagag cgattagacg tacagatagc tcatttttta aagaaaagcg
     2101 ttcaacatcg taggcgctat aaggtattaa aaataacaga aatcgtggca ggtttcctca
     2161 tagctgtctt ttgtgctatt cctatgccag gtgatcgcta ccgtttgatt tcggttgcct
     2221 tatccagtct cggcttgctg tgtgagggga ttatcaattt gtataatgca aaggaaaatt
     2281 ggatttctta ccaaaaaact gcgcaactcc tggagaaaga aaaattcctc tatcaatgcc
     2341 aaacggagaa atatgcagga aagaccaagg cttttgccct atttgtcaag acatgcgaag
     2401 gtcttatctc agaggagatt aaccagtggg aaagtatcca gtcaaaagaa gtggcagcta
     2461 gtgcagatgc tccagttaaa aaagagtagg aggtagagga aatgtctcaa tccagctacc
     2521 tgtcgccctt gctctggtta aaaaaagaag ccgataagga aaagatgagc gcgacccagt
     2581 gccagatatt tttcttttac tatcaaatgt ttgagctctt atttgctaga gaaagcgaca
     2641 tgaaagactt atgtctggga acgaaaggtt tttatttctc gcagttagag aaaaatttgc
     2701 tttctggagt ttcccgattt ctaaaaaact tggaggggaa agtaactctc aaggctaacc
     2761 aagaagtatc agctcgcaaa gccctttttc tagccttgac aactagccaa tcagattggc
     2821 aggagttagc tcctgttttt gatttttatc agactatcgg gaggcttgaa aatccttctc
     2881 tcttgagttc tcaggacaga caacatctga tgtggattta ccagtcagct ttggagaagg
     2941 attatattgt caaggttatt ggcgacaagc attttgtatt gaagagacaa gatgctacta
     3001 aattgacagc gcgccaaact caaactttgg aaattctgag tcaatcagaa gacttggtca
     3061 atcctgtcta tgttacatta ggagaaaagg gggtgctctt gcttgattaa gagaggagat
     3121 gttgtagctc tttatttgcc ttttccgact attagtagcg atttggctgt gaagaatcat
     3181 atgtatatct gtattgacaa cagcatgact aaaaacaaag agttggttaa aaatcagacc
     3241 ttcaaaccag ctctcttgac cagacgtttg gtcaagaact ttatgataga agagccggat
     3301 ctagctcgta atccttttac aagaccaacc ttgattgact tagataaggt atttatgttg
     3361 gataatacgg ttattccgac ttcttatcta gccagacggc gacgcaatgt ctcagaagaa
     3421 ttgtacgagg aaattttgga tcacttagtc caaccacggc tgatttcgct gaacaagtct
     3481 gagtttatgc aactcaatcc aggaacttat taggaggtag aagatggcaa ataaagcagt
     3541 aaatgacttt atactagcta tgaattacga taaaaagaaa ctcttgaccc atcagggaga
     3601 aagtattgaa aatcgtttca tcaaagaggg taatcagcta cccgatgagt ttgttgttat
     3661 cgaaagaaag aagcggagct tgtcgacaaa tacaagtgat atttctgtaa cagctaccaa
     3721 cgacagtcgc ctctatcctg gagcacttct cgtagtggat gagaccttgt tagagaataa
     3781 tcccactctt cttgcggtcg atcgtgctcc gatgacttat agtattgatt tgcctggttt
     3841 ggcaagtagc gatagctttc tccaagtgga agatcccagc aattcaagtg ttcgcggagc
     3901 ggtaaacgat ttgttggcta agtggcatca agattatggt caggtcaata atgtcccagc
     3961 tagaatgcag tatgaaaaaa tcacggctca cagcatggaa caactcaagg tcaagtttgg
     4021 ttctgacttt gaaaagacag ggaattctct tgatattgat tttaactctg tccattcagg
     4081 cgaaaagcag attcagattg ttaattttaa gcagatttat tatacagtca gcgtagacgc
     4141 tgttaaaaat ccaggagatg tgtttcaaga tactgtaacg gtagaggatt taaaacagag
     4201 aggaatttct gcagagcgtc ctttggtcta tatttcgagt gttgcttatg ggcgccaagt
     4261 ctatctcaag ttggaaacca cgagtaagag tgatgaagta gaggctgctt ttgaagcttt
     4321 gataaaagga gtcaaggtag ctcctcagac agagtggaag cagattttgg acaatacaga
     4381 agtgaaggcg gttattttag ggggcgaccc aagttcgggt gcccgagttg taacaggcaa
     4441 ggtggatatg gtagaggact tgattcaaga aggcagtcgc tttacagcag atcatccagg
     4501 cttgccgatt tcctatacaa cttctttttt acgtgacaat gtagttgcga cctttcaaaa
     4561 tagtacagac tatgttgaga ctaaggttac agcttacaga aacggagatt tactgctgga
     4621 tcatagtggt gcctatgttg cccaatatta tattacttgg aatgaattat cctatgatca
     4681 tcaaggtaag gaagtcttga ctcctaaggc ttgggacaga aatgggcagg atttaacggc
     4741 tcactttacc actagtattc ctttaaaagg gaatgttcgt aatctctctg tcaaaattag
     4801 agagtgtacc gggcttgctt gggaatggtg gcgtacggtt tatgaaaaaa ccgatttgcc
     4861 actagtgcgt aagcggacga tttctatttg gggaacaact ctctatccgc aggtagaaga
     4921 taaggtagaa aatgactagg agaggagaat gcttgcgaca aaaagaggcg atgatctctc
     4981 tgcggatatc tgaaagttta tctcttgcct agcgatttcc attggagtat gctcgtgatg
     5041 ttaaaatgaa tctatcaaac agaatgtgta aaaaagtaaa aagggtagcc aattccttga
     5101 tatacagggg attgattatc ctttacttgt tttagatgat tggaaattca agaacgacac
     5161 gtatcggaaa tattttgctg tttatatcta atacccattt atggaaaaaa tgaaattaaa
     5221 tatgaacaaa tcaattgcta gcaatggttt agaagtgcca gtgtactagt ctaacttctc
     5281 catcttggga acacgtaaat tatactcttc gaaaatctct tcaaaccatg tcagccctat
     5341 ccgcaacctc aaaacagtgt tttgagcaac ctgaggctag ctttctagtt tgctctttga
     5401 tttttattga gtattagaga tttgcttaga aactcttcta ttgatttctt agaatacaat
     5461 agcgcctata agaactgttg gatttcaaag tatttacttg aactatgata tgtctggagt
     5521 agactgtcaa caaaaggtgc agtatgattg tttttgtcgc ttatatctac aaacttcaaa
     5581 cagtaatttg agctgacttt gtcaaacttg gctgaaactt tctatttgca gataatggtc
     5641 tagtttgttt tttgaatgtg gatgagtgtc tgttgataaa aagatagaaa ggcaagacca
     5701 tcttagcagg taaagtgcta tcctagcttg atttgctaat aaaatccatt tcctctagtg
     5761 aaaatcaaaa aaacttgtgt tataataaga aagattaaaa tgtgaaaaaa ggagattcct
     5821 aatgggacgt aaatgggcca atatcgtagc caagaaaacg gctaaagatg gagccaactc
     5881 taaagtatat gcaaaatttg gtgtagaaat ctatgtagca gctaaaaaag gtgatccaga
     5941 tccagaatca aactcagctt tgaaattcgt tatcgaccgt gctaaacaag cccaagtgcc
     6001 aaaacacatt atcgataaag cgattgataa agccagagga aacacagacg aaacctttac
     6061 agaaggacgt tacgaaggtt ttgggccaaa tggctctatg ctaattgtgg atactttgac
     6121 ttctaacgtc aaccgtacag cagccaatgt ccgtgcagcc tttggtaaaa acggcggaaa
     6181 catgggcgct tcaggttctg tttcttacct ctttgacaac aaaggtgtta ttgtatttgg
     6241 aggtgaagat gcggacgcag tctttgagca attgctcgaa gcggatgtgg atgtggatga
     6301 cgtagaagca caagaaggta caatcacagt ttacacagcg ccaactgacc ttcacaaggc
     6361 tatcgttgcc ctccgtgagt ctggtattga agaattccaa gtgactgaat tggaaatgat
     6421 tcctcagtca gaagtggaat tgtcaggcga agaccttgaa acctttgaaa aactttacag
     6481 cgttcttgaa gacgacgaag acgtccaaaa gatttatacg aacgtagatg ggttttaatt
     6541 taacctatag aacatgatcc taagtgagaa aatcacttgg gattttttta atcaagaaaa
     6601 tagttctcat agaaatttaa ttattctcca gagaactatt tttatttaga agggggagat
     6661 tatgggtaaa ccgatgttag tctttaagcg ttttggtcat caaattcacc tgatggtgca
     6721 aaaggaagcc aaacgttgcg gcattgaatt tatgggtggg ccgcaaggtc aggttgtgcg
     6781 ttttttggat aatcgcgaga aaaaccaaga cttggtcttg attaaagata tcgagcagga
     6841 actcaatatt accaagtctg ttgctagtaa tctggttaag cgtatagtgc aaaatagttt
     6901 ggtggaattg gaggcgagtc ctgttgataa gcgggctaag tttgttcgtc tgatggacaa
     6961 agcacgttct cagatgcaac aggttaaggc tttttttgaa cgcatagaca agcagttgat
     7021 ggaagacatt gatgaagatg aattactgat ttttgagaaa gttctcggtc aactacaggc
     7081 aaaatatcaa gggaatagga ggagagaata aaaaaattag ccaaaagaat tagtagaaaa
     7141 gaatggggga tgattttact agccattctc tttactagcc attctcttta cttgcttttc
     7201 ggtctatcta gagttggaag tgccgaccta tatctcgaaa attacggatt tgctaggtag
     7261 tcaaggaact aatttagatg agttgtggca gccggcaagc atgatgatgg gaatgccctt
     7321 tcttgccttc ttgtccgtag ttgcagttgg attttttgca tcccgagtgg cggcttctta
     7381 tactagtagg ctgagaagtg atatttttaa ccgagttttg gattgctcgc agacagagat
     7441 taagaaattt tcaattccta gcctcttgac gcgtactacc aatgacatta ctcaagttca
     7501 aatgttgatt actatgggct tgcaagtggt aacgcgtggt ccaattatgg ctatctgggc
     7561 tattgggaag attttaggtc attcagaata ctgactctgg gccgtacttg tggcagtgat
     7621 tgtcaacgtc ctgatgacga ccgttttgat gacgctagcc tttccaaaac agtccttgat
     7681 tcaggggctg acagataaac ggaacagtat cactcgtgag agtttaacag gtattcgtgt
     7741 cgttcgtgca ggattatcaa aatgaaaaat ttgcagcagt aaatgatgaa ttgacccgtt
     7801 tgaatttgtt tgtcaaccgt cttatggcta ttttgaatcc tatcatgatg gggatttcaa
     7861 gtggtttgag tgtagcgatt tactggattg gggcctatgt gattaacgac gctgctccga
     7921 tagcgcgtct gcctctcttt agtgacatga ttattttcat gtcttatgcc atgtaggttg
     7981 tcatgggctt ccttctcatg ggagcactct tcatcgttct tccccgaact atggtctctg
     8041 ctaagcggat taatcaagtt ttagatttgc attcttctat ccaaaaccct gttcaagtac
     8101 agctgactga tgaaaacttc aaaggtcagg tcgagtttaa ggatgtgacc ttccgctatg
     8161 cggcaaattc ggaggcagtt attgaacatg ttagctttaa agcagaaact ggtcaaacag
     8221 tggcctttat tgggtcaaca ggttctggta aatcaactct ggtcaatctg attccacgtt
     8281 tctacgacgt gtcagcagga gaaattctgg tggacggtgt caatgttcaa gactatgact
     8341 tctctgcgac agctcatgct ggtcaaaagg ttgccattgt tgggccgact gggactggta
     8401 agacaaccat tgtcaatctt ttgatgaaat tctatgagat tgataaggga agtattcaca
     8461 ttgatggtgt ggataccaag gctatgacgc gttcagaagt gcatgatgcc ttttcaatgg
     8521 tcttgcagga tacctggctc tttgaaggaa ctattcgaga caatctcatc tataatcaaa
     8581 tagggattag tgatgaacga atgatggaag ctagtaaggc tgtgggaatt caccacttta
     8641 ttatgacctt gccagatggc tatgatacca tcttggatga caccgtgacc ttgtctgtag
     8701 gacaaaaaca actattgact attgctcgtg cccttcttaa ggatgcaccg cttttgattt
     8761 tggatgaggc gacttcttct gttgacacac ggacagagga attgatccaa aaagccatgg
     8821 accgtttgat ggaaggacgc acatcctttg tcattgccca ccgcttgtca accatccgaa
     8881 atgcagactt gatcttggtc atgaaagatg gaaatatcat cgagcaaggc aactatgagg
     8941 aactgatggc gcaaggtggc ttctacgctg acttgtacaa tagtcaattt acagaagatg
     9001 aagcagaaga ataaatcaaa agaaggcttg acggccttct ttttcttatt tatactagta
     9061 cacatagaaa gagagaagta tgagtcagaa attatacaat atgaaatttg ctgctgtcta
     9121 cttagccttg attgcaaaag ttgagcgaaa gggtggaaag gcagagtcag ttcaccaagt
     9181 gaccagttgg ttgacagggt atgaagtgag tgatgttctt gcttgtctgg atagggatgt
     9241 tacttacgga gattttttta gacaagcacc atattatgtc cctgaacgaa ttgcaattac
     9301 agggaagatt tgcggtgtcc gtattgagga aattgatgat cctctgatgc aggaaatacg
     9361 tcgcttggac aagctagtgg attggcttgc caaggggaaa acatctcaac aagttctaga
     9421 aaagtatgag aagcataaat gaaaaattat caagaatggt atcgaaatat cagctccaga
     9481 ctaactagtc gtccaattct tttattccta ttacgcagtt tcaatcgttt gatgacagtc
     9541 gccatgcccc tggtctatct gatcttgcta gtcaccactt acctgcaact aggacttggt
     9601 cagcaagttg gggtttatgt gcttattcct gcatcaggtt ttgtggcctt gtccctgttt
     9661 cgtaagaaaa tcaatcaccc gagaccttat gaaacttggg atatctgtcc ccttcttgat
     9721 aaggatagtt cgggacggtc gatgcccagt cgccatgtct tttcggcaac tatcatctcc
     9781 atggcctgtc tgcatgctag tctacctatt ggcttagtat gcttgatctt ttcagctttg
     9841 ctggggttgg tgagggtttt agggggtgtt cattatccca aggatgtctt ggttggctat
     9901 gcttgtggtt tggtgtgggg attccttttc ttcctattct gacatgtaag ttaagctagt
     9961 cctacaacca cttactgctt caaattgacc acttgctttt ttgcccttgt attcctaact
    10021 tagctttgat atagtagagt cagaaaggag atgtgatgaa gttacgaatt gagattgacg
    10081 gcaatttaga ggagactgaa attgtcatca agacccccac tttgacagat gaaattgcag
    10141 acttgcaacg gctcttgcaa gagtcaaagg ctccgaggtt gactttttac aaggggacag
    10201 gtgaatatta tctagacctg tcagaaattc tcttctttga aacagaaggg agcaagatct
    10261 acgctcataa ccagaaggaa gcttatgagg ttcgcctcaa gctctatgag ttggagtcta
    10321 tcttgcctcg ctattttaat cgagtttcca agtcaacgat cgcaaacatc cgtcagattt
    10381 actcagtgga caagtccttt tcaggaacgg gcaccatttc cttttatcag acgcacaagg
    10441 aggttcatgt ctcacggcat taccaatccc tcctaaaaga aaatctaaga aacatgaggt
    10501 aaaaaacatg aaaaagaaag catttggtat tgttttattg gttttagcag cttggatctt
    10561 gctgcaaggg aattttggaa ttccttcttt ggatggtaaa atatggcctt tactaggtat
    10621 tgtttttttt gcttataagt ccattgagtc catccttaga cgtcatctca cttcggcagt
    10681 ttttacaggt ttactggcgc tcatcattgc aaattacgct tatgacttgt taccagttac
    10741 caatcattct cttatttggg ctagcatctt ggtggtactt ggtgttggtt atctgacgca
    10801 ttcaagtaag ttctggaatg aaaaaaaatg gtggtacaat gggaaaaaaa cagtcgtcac
    10861 ggataaggaa gtcgcttttg gtagcgggac cttctataag caagatcaag atctcgtaga
    10921 tgaccaagtg gaagtcgctt ttggggatgc taaaatctac tatgataatg cagagatgct
    10981 aggtgatttt gcaactttaa atattgaagt ggccttcgga aatgcaaccg tctatgttcc
    11041 acaacactgg cgtgtcgatt tgaaagtaga aacctccttt ggtgcagcta aggctgacgc
    11101 tcctgtagcc ccaaccagca aaaccttgat tatccgtgga gatgtggctt ttgggaagtt
    11161 ggaaattgtc tacgttaaat aaaaaatctt cacttcaacc atcaaaatag gcgtactaag
    11221 agtaggaaat tgatgccttg ctctgatttc agttctatgg ttgttagact ttaaaaaatg
    11281 aaatgttgcc tttaaaagtt gtatattttt cgatattttg actcttacgt ttgatgtatc
    11341 tatgtcctac agcgtggatg atgtagtgta aaatgcttta aaaaaaaaat gaatgatatt
    11401 ggacagtttt tttgaacttt taattgctca ggagccatga aagcaagcgc ttgggtttat
    11461 gacaagggag aatggtatta tgtaagttct tctggttcca tgattgcgaa tgattgggtc
    11521 aaagacaatg gcaagtagta ttatctggct tcatcaggta agatgcttcg caatacctac
    11581 acacctgata gttactacgt tggcaactta ggtgcctggc aataagaata atactcttcg
    11641 aaaatctctt caaaccacgt cagcttcatc cacaacctca aaacagtgtt ttgagctgac
    11701 ttcgtcagtt ctatctacaa cctcaaagca gtgctttgag cagcctgcgg ctagcttcct
    11761 agtttggtct ttgattttca ttgagtataa gaagtccttt tccttaaagg aagaggcttt
    11821 ttacttgctt ttttgctgtt tcctcatttg tcctaaagcc ttttttgtgt taaaatgaat
    11881 ggtatgaaag ctaaaaaaat gtggatggca ggtctggctc tgcttggtat tgggagcctt
    11941 gcccttgcta cgaaaaaagt tgcagatgac cataagctca tgaagactca ggaagagttg
    12001 acagcgattg tacgagacca tttttccgac atgggggaaa ttgcgaccct ctatgttcaa
    12061 gtttacgaaa gcagtctgga gagcttggtt ggtggcgtca tttttgagga tggccgtcat
    12121 tatacctttg tctatgaaaa tgaagaccta gtctatgagg aggaagtctt atgatacagc
    12181 cagcaagttt agaagaatta gcatctttag tggaaaaagc gggcaagaag gtcttccttt
    12241 ttgtggcaga ctggtgtggc gattgtcgtt atatttatcc tgccttacca gagattgagg
    12301 agaccaatcc agagttcacc tttattcgaa tggaccgaga tcagtatatg gatttggcca
    12361 aactctggga tgtttacgga attcctagcc ttgttgttct agaaaaggac aaggaaatcg
    12421 gtcgttttgt caatcgcgac cgtaaaagta aggagcaaat taacgatttt ttagcaggat
    12481 tgaaatagga gaaaaaggaa acaatgattt ttacgtataa caaagaacat gtcggtgatg
    12541 tccttatggt catcgtgaaa aatagcggag atgccaaact gaatgtggaa cgcaaaggca
    12601 aggtagcccg tgttttcctc aaagaaaatg gggaaacagt agcttggaac atttttgagg
    12661 tttcgagctt gtttgaaatt gcagagcgcg gtcaagtctt tttatcagat gagcaagtcg
    12721 ctcgtttgaa ccaagaatta caggcggaag gttttacaga agaaattgtt aatgataagg
    12781 aacctaagtt tgttgttggt gaaattgtcg agatggtagc tcatccagac agtgaccacc
    12841 tcaacatctg ccaagttgca gtcgcaagtg acaagatagt gcaaatcgtt gcaggagcac
    12901 ctaatgcgcg tgttgggttg aaaaccattg tggctcttcc tggtgccatg atgccaaaag
    12961 ggaatttcat tttcccaggc gaacttcgtg gtgaaaagag ttttgggatg atgtgtagtc
    13021 ctcgtgaatt gcatttgcca aatgctccgc aaaaacgtgg ggtgcttgaa ttatcagaag
    13081 accaagttgt cggaacaccg ttcgacccag cgaaacactg gactgcctag gaacttgtca
    13141 gcatctgata gaaggaaata agatggcaaa aaatgtagtg attacgggag caacctcagg
    13201 aatcggtgaa gcgattgcgc gtgcttatct ggagcagggt gaggatgtcg ttctaacagg
    13261 acgacggata gacagattag aaatcctcaa gtcggagttt gcagtaagct ttccaaatca
    13321 aacagtttgg acttttctcc tagatgtcac ggatatgaca atggtaaaga ctgtctgctc
    13381 tgatatttta gaaacgatag gtcagattga catcctggtc aataacgcag gactggctct
    13441 tggcttagct ccctatcaag actatgaaga gttggatatg ctgaccatgt tggataccaa
    13501 tgtcaaaggt ttgatggcag tcactcgctg tttcttgcca gcaatggtaa aagccaatca
    13561 aggtcatatt atcaatatgg ggtcaaccgc aggaatttat gcctatgctg gtgcagctgt
    13621 ttactcagcc accaaggcgg cagttaagac cttttcagat ggactgcgaa ttgataccat
    13681 cgcaacggat atcaaggtga caaccattca gcctgggatt gtcgaaacag atttctcaac
    13741 tgttcgtttt catggtgata aagagcgggc tgcgtccgtt taccaaggaa tagaagcctt
    13801 gcaagctcag gatattgcag acacagtagt ctatgtgacc agtcagcctc gccgtgttca
    13861 gattacagat atgaccatta tggccaatca acaggcgaca ggtttcatga ttcataaaaa
    13921 ataagaaatt tcctcgaaaa gttacaaatt tctgtaactt tttttgattt cctacgaata
    13981 gataagtagg aggaagaaaa tatgtataat aaagttatct tgattgggcg tttaacgtct
    14041 acaccagaat tgcacaaaac caacaatgac aagtcggtag cgcgagcaac tatcgctgtg
    14101 aaccgtcgtt acaaagacca aaacggtgaa cgtgaagctg attttgtcaa tatggtccta
    14161 tggggcagac tagcagaaac tttggcaagc tacgcaacca aaggtagtct catttccgtt
    14221 gatggagaat tgcgtacccg tcgctttgag aaaaatggcc aaatgaacta cgtgaccgaa
    14281 gtacttgtca caggattcca actcttggaa agtcgtgccc aacgtgctat gcgtgaaaat
    14341 aatgcaggcc aagatttggc agatttggtc ttggaagagg aagaattgcc attttaagaa
    14401 ttaaaaagtc tgagttggtc tcaggctttt tatcttgaga aagtcagact tttttcttga
    14461 ttatttctga ctaagtgata caatagaact atgaattagc actcaagttc aaagagtgct
    14521 aataatatct atctcattat ggaggaaatc agatgttgaa accattaggg gaccgtgtgg
    14581 tcttaaaaat agaagaaaaa gaacaaactg ttggaggctt tgtccttgca ggctcagccc
    14641 aagaaaaaac caaaacagct caagttgtgg ctactggaca aggtgttcgt accttgaacg
    14701 gtgacttggt tgctccaagt gttaaaactg gagatcgtgt cttagttgaa gcccacgcag
    14761 gtcttgatgt caaagatggc gatgaaaagt acatcatcgt aggcgaagct aacattttgg
    14821 caatcattga ggaatagaag gagaaagtaa gtatgtcaaa agaaattaaa ttttcatcag
    14881 atgcccgttc agccatggtt cgtggtgtcg atatccttgc agacactgtt aaagtaacct
    14941 tgggaccaaa aggtcgcaat gtcgttcttg aaaagtcatt cggttcaccc ttgattacca
    15001 atgacggtgt gaccattgcc aaagaaatcg aattggaaga ccattttgaa aatatgggtg
    15061 ctaagttagt atcagaagta gcttctaaaa ccaatgatat cgcaggtgac ggaactacga
    15121 ctgcaacagt cttgacccaa gctatcgtcc gtgaaggaat caaaaacgtc acagcaggtg
    15181 caaatccaat cggtattcgt cgtgggattg aaacagcagt tgccgcagca gttgaagctt
    15241 tgaaaaacaa cgccatccct gttgccaata aagaagctat cgctcaagtt gcagccgtat
    15301 cttctcgttc tgaaaaagtt ggtgagtaca tctctgaagc aatggaaaaa gttggcaaag
    15361 acggtgtcat caccatcgaa gagtcacgtg gtatggaaac agagcttgaa gtcgtagaag
    15421 gaatgcagtt tgaccgtggt tacctttcac agtacatggt gacagatagc gaaaaaatgg
    15481 tggctgacct tgaaaatccg tacattttga ttacagacaa gaaaatttcc aatatccaag
    15541 aaatcttgcc acttttggaa agcattctcc aaagcaatcg tccactcttg attattgcgg
    15601 atgatgtgga tggcgaggct cttccaactc ttgttttgaa caagattcgt ggaaccttca
    15661 acgtagtagc agtcaaggca cctggttttg gtgaccgtcg caaagctatg cttgaagata
    15721 tcgccatctt aacaggcgga acagttatca cagaagacct tggtcttgag ttgaaagatg
    15781 cgacaattga agctcttggt caagcagcga gagtgaccgt ggacaaagat agcacggtta
    15841 ttgtagaagg tgcaggaaat cctgaagcga tttctcaccg tgttgcggtt atcaagtctc
    15901 aaatcgaaac tacaacttct gaatttgacc gtgaaaaatt gcaagaacgc ttggccaaat
    15961 tgtcaggtgg tgtagcggtt attaaggtcg gagccgcaac tgaaactgag ttgaaagaaa
    16021 tgaaactccg cattgaagat gccctcaacg ctactcgtgc agctgttgaa gaaggtattg
    16081 ttgcaggtgg tggaacagct cttgccaatg tgattccagc tgttgctacc ttggaattga
    16141 caggagatga agcaacagga cgcaatattg ttctccgagc tttggaagaa cctgttcgtc
    16201 aaatcgccca caatgcagga tttgaaggat ctatcgttat cgatcgtttg aagaatgctg
    16261 aagttggtac aggcttcaac gcagcaactg gcgagtgggt caacatgatt gaagagggaa
    16321 tcatcgaccc agttaaagtg agtcgttcag ctctacaaaa tgcagcatct gtagccagct
    16381 tgattttgac tacggaagca gttgtagcca ataaaccaga accagtggcc ccagctccag
    16441 caatggatcc aagcatgatg ggcggcatga tgtaagtttt ctatagaaaa caacttataa
    16501 aaaacacaaa aggagggaat gcctacccct cctttttatg ctattcactt ctaaattgat
    16561 ttgagctctc ctaacttata tgataaaata agactagaaa aaggagaaga acatgatcga
    16621 tgtagaagaa attctgagca agatgacccc caatcagaag attaattatg accgtgtcat
    16681 gcagaagatg gtacaagtat gggagaaaaa tgagcaacgt ccaactattc tcatgcacgt
    16741 ttgctgtgcc ccttgtagta cctatacact agaatatttg accaagtatg cagatgtgac
    16801 catctatttt gccaattcta atatccatcc caaggtagaa taccataagc gggtctatgt
    16861 cactaagaaa tttgttagtg attttaatga gcggacagga aatacggttc agtacctaga
    16921 agctccctac gaacccaatt aataccgaaa actagttagg ggactagagg aggagcccga
    16981 aggtggcgac cgttgcaagg tttgttttga ctaccgactg gataaaacag cgcaagtggc
    17041 tatggacttg ggctttgact actttggttc agccttgacc atcagtcctc ataagaattc
    17101 tcaaaccatc aatagcatcg gaatcgatgt gcaaaaaatt tacacgaccc actatcttcc
    17161 cagcgatttc aagaaaaatc aaggctacaa acgttcagta gagatgtgtg aggagtatga
    17221 tatctatcgt caatgttatt gtggctgcgt ctatgcagcc caagcccaga atattgacct
    17281 ggttcaggtt aagaaggacg ccacagcgtt catgcttgat aaggatgttg aaaaagacta
    17341 ttctcatatc aaatttatag tagattgaaa ctagaatagt acacctttac ttctaaaaca
    17401 ttgttagaaa tcgatttgac tgtccttatt tcattttact atactgttac gaaattagat
    17461 atataaatga aaacttgcct caaggtaggt tttttgctag tagaaaagcg atagacagaa
    17521 attgttggac tcttaaaaaa atgaagtttt gctcaaaaga gcttcttaaa ttaaaacgag
    17581 caattatcag ttcaaatctg tttcaaaaga agaacggata aattaatcga tgctttgttt
    17641 ttgagttagg aagttaagca tgccaaactg agtcgtagga ataaatttct ttatagaaat
    17701 gtgtt
//
LOCUS       DAVLVP010000034        17011 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.034, whole genome shotgun sequence.
ACCESSION   DAVLVP010000034 DAVLVP010000000
VERSION     DAVLVP010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 17011)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17011)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17011
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.034"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            90..5393
                     /locus_tag="VYN69_001102"
     CDS             90..5393
                     /locus_tag="VYN69_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001032503.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HEV1533385.1"
                     /translation="MNKGLFEKRCKYSIRKFSLGVASVMIGAAFFGTSPVLADSVQSG
                     STADLPADLATALATAKENDGRDFEAPKVEEDQGSPEVTDGPKTEEELLALEKEKPAE
                     EKPKEDKPAAAKPETPKTVTPEWQTVEKKEQKGTVTIREEKGVRYNQLSSTAQNDNAG
                     KPALFEKKGLTVDANGNATVDLTFKDDSEKGKSRFGVFLKFKDTNNNVFVGYDKDGWF
                     WEYKSPTTSTWYRGSRVAAPETGSTNRLSITLKSDGQLNASNNDVNLFDTVTLPAAVN
                     DHLKNEKKILLKAGSYGNDRTVVSVKTDNQEGVKADDTPAQKETGPVVDDSKVTYDTI
                     QSKVLKAVIDQAFPRVKEYTLNGHTLPGQVQQFNQVFINNHRITPEVTYKKINETTAE
                     YLMKIRDDAHLINAEMTVRLQVVDNQLHFDVTKIVNHNQVTPGQKIDDERKLLSSISF
                     LGNALVSVSSDQTGAKFDGATMSNNTHVSGDDHIDVTNPMKDLAKGYMYGFVSTDKLA
                     AGVWSNSQNSYGGGSNDWTRLTAYKETVGNANYVGIHSSEWQWEKAYKGIVFPEYTKE
                     LPSAKVVITEDANADKKVDWQDGAIAYRSIMNNPQGWEKVKDITAYRIAMNFGSQAQN
                     PFLMTLDGIKKINLHTDGLGQGVLLKGYGSEGHDSGHLNYADIGKRIGGVEDFKTLIE
                     KAKKYGAHLGIHVNASETYPESKYFNEKILRKNPDGSYSYGWNWLDQGINIDAAYDLA
                     HGRLARWEDLKKKLGDGLDFIYVDVWGNGQSGDNGAWATHVLAKEINKQGWRFAIEWG
                     HGGEYDSTFHHWAADLTYGGYTNKGINSAITRFIRNHQKDAWVGDYRSYGGAANYPLL
                     GGYSMKDFEGWQGRSDYNGYVTNLFAHDVMTKYFQHFTVSKWENGTPVTMTDNGSTYK
                     WTPEMRVELVDADNNKVVVTRKSNDVNSPLYRERTVTLNGRVIQDGSAYLTPWNWDAN
                     GKKLSTDKEKMYYFNTQAGATTWTLPSDWAKSKVYLYKLTDQGKTEEQELTVKDGKIT
                     LDLLANQPYVLYRSKQTNPEMSWSEGMHIYDQGFNSGTLKHWTISGDASKAEIVKSQG
                     ANDMLRIQGNKEKVSLTQKLTGLKPNTKYAVYVGVDNRSNAKASITVNTGEKEVTTYT
                     NKSLALNYVKAYAHNTRRDNATVDDTSYFQNMYAFFTTGSDVSNVTLTLSREAGDQAT
                     YFDEIRTFENNSSMYGDKHDTGKGTFKQDFENVAQGIFPFVVGGVEGVEDNRTHLSEK
                     HDPYTQRGWNGKKVDDVIEGNWSLKTNGLVSRRNLVYQTIPQNFRFEAGKTYRVTFEY
                     EAGSDNTYAFVVGKGEFQSGRRGTQASNLEMHELPNTWTDSKKAKKATFLVTGAETGD
                     TWVGIYSTGNASNTRGDSGGNANFRGYNDFMMDNLQIEEITLTGKMLTENALKNYLPT
                     VAMTNYTKESMDALKEAVFNLSQADDDISVEEARAEIAKIEALKNALVQKKTALVADD
                     FESLDAPAQPGEGLENAFDGNLSSLWHTSWGGGDVGKPATMVLKEPTEITGLRYVPRG
                     SGSNGNLRDVKLVVTDESGKEHTFTTTDWPDNNKSKDIDFGKTIKAKKIVLTGTKTYG
                     DGGDKYQSAAELIFTRPQVAETPLDLSGYEAALAKAQKLTDKDNQEEVASVQASMKYA
                     TDNHLLTERMVEYFADYLNQLKDSATKPDAPTVEKPEFKLSSLASDQGKTPDYKQEID
                     RPETPEQILPATGESQSDTALFLAGVSLALSALFVVKTKKD"
     gene            complement(5559..7718)
                     /locus_tag="VYN69_001103"
     CDS             complement(5559..7718)
                     /locus_tag="VYN69_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEV1533386.1"
                     /translation="MNKPTILRLIKYLSISFLSLVIAAIVLGGGVFFYYVSKAPSLSE
                     SKLVATTSSKIYDNKNQLIADLGSERRVNAQANDIPTDLVKAIVSIEDHRFFDHRGID
                     TIRILGAFLRNLQSNSLQGGSTLTQQLIKLTYFSTSTSDQTISRKAQEAWLAIQLEQK
                     ATKQEILTYYINKVYMSNGNYGMQTAAQNYYGKDLNNLSLPQLALLAGMPQAPNQYDP
                     YSHPEAAQDRRNLVLSEMKNQGYISAEQYEKAVNTPITDGLQSLKSASNYPAYMDNYL
                     KEVINQVEEETGYNLLTTGMDVYTNVDQEAQKHLWDIYNTDEYVAYPDDELQVASTIV
                     DVSNGKVIAQLGARHQSSNVSFGINQAVETNRDWGSTMKPITDYAPALEYGVYDSTAT
                     IVHDEPYNYPGTNTPVYNWDRGYFGNITLQYALQQSRNVPAVETLNKVGLNRAKTFLN
                     GLGIDYPSIHYSNAISSNTTESDKKYGASSEKMAAAYAAFANGGTYYKPMYIHKVVFS
                     DGSEKEFSNVGTRAMKETTAYMMTDMMKTVLSYGTGRNAYLAWLPQAGKTGTSNYTDE
                     EIENHIKTSQFVAPDELFAGYTRKYSMAVWTGYSNRLTPLVGNGLTVAAKVYRSMMTY
                     LSEGSNPEDWNIPEGLYRNGEFVFKNGARSTWSSPAPQQPPSTESSSSSSDSSTSQSS
                     STTPSTNNSTTTNPNNNTQQSNTTPDQQNQNPQPAQP"
     gene            complement(7715..8311)
                     /gene="recU"
                     /locus_tag="VYN69_001104"
     CDS             complement(7715..8311)
                     /gene="recU"
                     /locus_tag="VYN69_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEV1533387.1"
                     /translation="MVNYPHKVSSQKRQTSLSQPKNFANRGMSFEKMINATNDYYLSQ
                     GLAVIHKKPTPIQIVQVDYPQRSRAKIVEAYFRQASTTDYSGVYHGYYIDFEVKETKQ
                     KRAIPMKNFHPHQIQHMEQVLAQQGICFVLLHFSSQQETYLLPAFDLIRFYHQDKGQK
                     SMPLEYIREYGYEIKAGAFPQIPYLNVIKEHLLGGKTR"
     gene            8377..8904
                     /locus_tag="VYN69_001105"
     CDS             8377..8904
                     /locus_tag="VYN69_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEV1533388.1"
                     /translation="MATALVLGYSAFDLGLFSDKDPRLKLIKKAIRKDLEAMAADGVS
                     WLVFTGSLGFEYWVLEVAQEMKTEYGFQLATIFAFETHGENWNEGNQMKLSRFKQVDF
                     VKYAYPRYEHKGQLRDYQQFLLENTTSSYLFYDEENETKLAYFYQKMKNQEDYFIKRL
                     TFDELNELAENFSEN"
     gene            8974..9303
                     /gene="gpsB"
                     /locus_tag="VYN69_001106"
     CDS             8974..9303
                     /gene="gpsB"
                     /locus_tag="VYN69_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000146522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEV1533389.1"
                     /translation="MASIIFSAKDIFEQEFGREVRGYNKVEVDEFLDDVIKDYETYAA
                     LVKSLRQEIADLKEELTRKPKPSPVQAEPLEAAITSSMTNFDILKRLNRLEKEVFGKQ
                     ILDNSDF"
     gene            9324..9704
                     /gene="rnpB"
                     /locus_tag="VYN69_001107"
     ncRNA           9324..9704
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VYN69_001107"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9789..10946
                     /locus_tag="VYN69_001108"
     CDS             9789..10946
                     /locus_tag="VYN69_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEV1533390.1"
                     /translation="MKKEFNLIATVAAGLEAVVGREVRELGYDCQVENGRVRFQGDVR
                     AIIETNLWLRAADRIKIIVGTFPAKTFEELFQGVFALDWENYLPLGARFPISKAKCVK
                     SKLHNEPSVQAISKKAVVKKLQKHYARPEGVPLMENGPEFKIEVSILKDVATVMIDTT
                     GSSLFKRGYRTEKGGAPIKENMAAAILQLSNWYPDKPLIDPTCGSGTFCIEAVMIARK
                     MAPGLRRSFAFEEWNWISDRLIQEVRTEAAKKVDRELELDIMGCDIDARMVEIAKANA
                     QAAGVAGDITFKQMRVQDLRSDKINGVIISNPPYGERLSDDAGVTKLYAEMGQVFAPL
                     KTWSKFILTSDEAFESKYGSQADKKRKLYNGTLKVDLYQYFGQRVKRQEVK"
     gene            10959..12353
                     /gene="mapZ"
                     /locus_tag="VYN69_001109"
     CDS             10959..12353
                     /gene="mapZ"
                     /locus_tag="VYN69_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mid-cell-anchored protein MapZ"
                     /protein_id="HEV1533391.1"
                     /translation="MSKKRRNRHKKEAQEPQFDFDEAKELTVGQAIRKNEEVEAGVLP
                     EDSILDKYVKQHRDEIEADKFATRQYKKEEFVETQSLDDLIQEMREAVEKSEASSEEV
                     PSSEDILLPLPLDDEEQGLDPLLLDDENPTEMTEEVEEEQNLSRLDQEDSEKKSKKGF
                     ILTVLALVSVIICVSAYYVYRQVARSTKEIETSQSTTANQSDVDDFNTLYDAFYTDSN
                     KTALKNSQFDKLSQLKTLLDKLEGSREHTLAKSKYDSLVTQIKAIQDVNAQFEKLAIV
                     DGVLDTNAKAKSDAKFTDIKTGNTELDKVLDKAISLGKSQQTSTSSSSSSQTSSSSSS
                     QASSNTTSKPKPSSSNETRSSRSEVNMGLSSAGVAVQRSASRVAYNQSAIDDSNNSAW
                     DFADGVLEQILATSRSRGYITGDQYILERVNIVNGNGYYNLYKPDGTYLFTLNCKTGY
                     FVGNGAGHADDLDY"
     gene            12429..13853
                     /gene="gndA"
                     /locus_tag="VYN69_001110"
     CDS             12429..13853
                     /gene="gndA"
                     /locus_tag="VYN69_001110"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004185725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEV1533392.1"
                     /translation="MTKANFGVVGMAVMGRNLALNIESRGYTVAIYNRSKEKTEDVIA
                     CHPEKNFVPSYDVESFVNSIEKPRRIMLMVQAGPGTDATIQALLPHLDKGDILIDGGN
                     TFYKDTIRRNEELANSGINFIGTGVSGGEKGALEGPSIMPGGQKEAYELVADVLEEVS
                     AKAPEDGKPCVTYIGPDGAGHYVKMVHNGIEYGDMQLIAESYDLMQHLLGLSAEDMAE
                     IFTEWNKGELDSYLIEITADILSRKDDEGQDGPIVDYILDAAGNKGTGKWTSQSSLDL
                     GVPLSLITESVFARYISTYKEERVHASKVLPKPAAFNFEGDKAELIEKIRQALYFSKI
                     ISYAQGFAQLRVASKENNWNLPFADIASIWRDGCIIRSRFLQKITDAYNRDADLANLL
                     LDEYFLDVTAKYQQAVRDIVALAVQAGVPVPTFSAAITYFDSYRSADLPANLIQAQRD
                     YFGAHTYQRKDKEGTFHYSWYDEK"
     gene            13865..14554
                     /locus_tag="VYN69_001111"
     CDS             13865..14554
                     /locus_tag="VYN69_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000518023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1533393.1"
                     /translation="MGKRILLLEKERNLAHFLSLELQKEQYRVDLVEEGQKALSMALQ
                     TDYDLILLNVNLGDMMAQDFAEKLSRTKPASVIMILDHWEDLQEELEVVQRFAVSYIY
                     KPVLIENLVARISAIFRGRDFIDQHCSLMKVPRTYRNLRIDVEHHTVYRGEEMIALTR
                     REYDLLATLMGSKKVLTREQLLESVWKYESATETNIVDVYIRYLRSKLDVKGQKSYIK
                     TVRGVGYTMQE"
     gene            14652..15614
                     /gene="cbpC"
                     /locus_tag="VYN69_001112"
     CDS             14652..15614
                     /gene="cbpC"
                     /locus_tag="VYN69_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpC"
                     /protein_id="HEV1533394.1"
                     /translation="MKILKKMMQVLLAVFFFGLLATNTVFANTTGGRFVDKDNRKYYV
                     KDDHKAIYWHKIDGKTYYFGDGGERVVGWQYLEIPGTGYRDNLFDNQPVNEIGLQEKW
                     YYFGQDGALLEQTDKQVLEAKTSENTGKVYGEQYPLSAEKRTYYFDNNYVVKTGWIYE
                     DGNWYYLNKLGNFGDDSYNPLPIGEVAKGWTQDFHVTIDIDRSKPAPWYYLDASGKML
                     TDWQKVNGKWYYFGSSGSMATGWKYVRGKWYYLDNKNGDMKTGWYQDGSTWYYLDPSN
                     GDMKIGWTKVNGKWYYLNSNGAMVTGSQTIDGKVYNFASSGEWI"
     gene            15633..16631
                     /gene="cbpJ"
                     /locus_tag="VYN69_001113"
     CDS             15633..16631
                     /gene="cbpJ"
                     /locus_tag="VYN69_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline-binding protein CbpJ"
                     /protein_id="HEV1533395.1"
                     /translation="MKLLKKTMQAGLTVIFFGLLATNTVFADNSEGWQFVQENGRTYY
                     KKGDLKETYWLVIDGKYYYFDSLSGEMVVGWQYIPFPSKGSTIGPYPNGIRLEGFPKS
                     EWYYFDKNGVLQEFVGWKTLEIKTKDSVGRKYGEKREDSEDKEEKRYYTNYYFNQNHS
                     LETGWLYDQSNWYYLAKTEINGENYLGGERRAGWINDDSTWYYLDPTTGIMQTGWQYL
                     GNKWYYLRSSGAMATGWYQEGTTWYYLDQPNGDMKTGWQNLGNKWYYLRSSGAMATGW
                     YQDGSTWYYLNAGNGDMKTGWFQVNGNWYYAYSSGALAVNTTVDGYSVNYNGEWVR"
     gene            complement(16847..>17011)
                     /locus_tag="VYN69_001114"
     CDS             complement(16847..>17011)
                     /locus_tag="VYN69_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380 family transposase"
                     /protein_id="HEV1533396.1"
                     /translation="GKCVRTGRKQLLKLSSLYAYSELFSALYSRIRKVNLNLPVPYEP
                     PRKKASLMMH"
BASE COUNT         5195 a         3107 c         3921 g         4788 t
ORIGIN      
        1 agggttaagc attgcacttt cagaggaagg agtatttttt aaaaaggaaa tgtaaacgct
       61 tgctcaaaaa tgaaaggatt tagaagttta tgaataaagg attatttgaa aaacgttgta
      121 aatatagtat tcggaaattt tcattaggtg ttgcttctgt tatgattgga gctgcattct
      181 ttgggacaag tccggttctt gcagatagcg tgcagtctgg ttccacggcg gacttaccag
      241 ctgatttagc tactgctctt gcaacagcaa aagagaatga tgggcgtgat tttgaagcgc
      301 ctaaggtgga agaagaccaa ggttctccag aagttacaga tggacctaag acagaagaag
      361 aactattagc acttgaaaaa gaaaaaccgg ctgaagaaaa accaaaagag gataaacctg
      421 cagctgctaa acctgaaaca cctaagacgg taacccctga atggcaaacg gtagagaaaa
      481 aagaacaaaa gggaacagtc actatccgag aagaaaaagg tgtccgctac aaccaactat
      541 cctcaactgc tcaaaatgat aacgcaggta aaccagccct gtttgaaaag aagggcttga
      601 ccgttgatgc caatggaaat gcaactgttg atttaacctt caaagatgat tctgaaaagg
      661 gcaaatcacg ctttggtgtc ttcctgaaat ttaaagatac caataataat gtttttgtcg
      721 gttatgacaa ggatggctgg ttctgggagt ataaatctcc aacaactagc acttggtata
      781 gaggtagtcg tgttgctgct cctgaaacag gatcaacaaa ccgtctctct atcactctca
      841 agtcagacgg tcagctaaat gccagcaata acgatgtcaa tctctttgac acagtgactc
      901 taccagctgc ggtcaatgac catcttaaaa atgagaagaa gattcttctc aaggcgggct
      961 cttatggcaa tgaccgtacg gttgtcagcg ttaaaacaga caatcaagaa ggcgtaaaag
     1021 cggatgatac tcctgcccag aaagaaacag gtccggttgt tgacgatagc aaggtgactt
     1081 atgatacgat ccagtctaag gtacttaagg cagtaattga ccaagccttc cctcgtgtca
     1141 aggaatacac cttgaatgga catactttgc caggacaggt gcaacagttc aaccaagtct
     1201 ttatcaataa ccaccgaatc acccctgaag tcacttataa gaaaatcaat gagacaacag
     1261 cagagtactt gatgaagatt cgcgatgatg ctcacttaat caatgcggaa atgacagtac
     1321 gcttgcaagt tgtggacaat caattgcact ttgatgtgac caagattgtc aaccacaatc
     1381 aagtcactcc aggtcaaaag attgatgacg aaagaaaact actttcttct attagtttcc
     1441 tcggcaatgc tttagtctct gtttctagtg atcaaactgg tgctaagttt gatggggcaa
     1501 ccatgtcaaa caatacgcat gtcagcggag atgatcatat cgatgtaacc aatccaatga
     1561 aagacctagc caagggttac atgtatggat ttgtttctac agataagctt gctgctggtg
     1621 tttggagtaa ctctcaaaac agctatggtg gtggttcgaa tgactggact cgtttgacag
     1681 cttataaaga aacagtcgga aatgccaact atgtaggaat ccatagctct gaatggcaat
     1741 gggaaaaagc ttataagggc attgttttcc cagaatacac gaaggaactt ccaagtgcta
     1801 aggttgttat cactgaagat gccaatgcag acaagaaagt tgattggcaa gatggtgcca
     1861 ttgcttatcg tagcattatg aacaatcctc aaggttggga aaaagttaag gatatcacag
     1921 cttaccgtat cgcgatgaac tttggttctc aagcacaaaa cccattcctt atgaccttgg
     1981 atggtatcaa gaaaatcaat ctccacacag atggtcttgg gcaaggtgtt ctccttaaag
     2041 gatatggtag cgaaggccat gactctggtc acttgaacta tgctgatatt ggtaagcgta
     2101 tcggtggtgt cgaagacttc aagaccctaa ttgagaaggc taagaaatat ggagctcatc
     2161 taggtatcca cgttaacgct tcagaaactt atcctgagtc taaatacttc aatgaaaaaa
     2221 ttctccgtaa gaatccagat ggaagctata gctatggttg gaactggcta gatcaaggta
     2281 tcaacattga tgctgcctat gacctagctc atggtcgttt ggcacgttgg gaagatttga
     2341 agaaaaaact tggtgacggt ctcgacttta tctatgtgga cgtttggggt aatggtcaat
     2401 caggtgataa cggtgcctgg gctacccacg ttcttgctaa agaaattaac aaacaaggct
     2461 ggcgctttgc gatcgagtgg ggccatggtg gtgagtacga ctctaccttc catcactggg
     2521 cagctgactt gacctacggt ggctacacca ataaaggtat caacagtgcc atcacccgct
     2581 ttatccgtaa ccaccaaaaa gatgcttggg ttggggacta cagaagttat ggtggtgcag
     2641 ccaactatcc actgctaggt ggctacagca tgaaagactt tgaaggctgg caaggaagaa
     2701 gtgactacaa tggctatgta actaacctct ttgcccatga cgtcatgact aagtacttcc
     2761 aacacttcac tgtaagtaaa tgggaaaatg gtacaccggt gactatgacc gataacggta
     2821 gtacctataa atggactcca gaaatgcgag tggaattggt agatgctgac aataataaag
     2881 tagttgtaac tcgtaagtca aatgatgtca atagcccact ataccgtgaa cgtacagtaa
     2941 cgctcaacgg acgtgtcatc caagatggtt cagcttactt gactccttgg aactgggatg
     3001 caaatggtaa gaaactttct actgataagg aaaagatgta ctacttcaat acgcaggccg
     3061 gtgcaacaac ttggaccctt ccaagcgatt gggcaaagag caaggtttac ctttacaagc
     3121 taactgacca aggtaagaca gaagagcagg aactaactgt aaaagatggt aaaattaccc
     3181 tagatcttct agcaaatcaa ccatacgttc tctatcgttc gaaacaaacc aatcctgaaa
     3241 tgtcatggag tgaaggcatg cacatctatg accaaggatt taatagcggt accttgaaac
     3301 attggaccat ttcaggcgat gcttctaagg cagaaattgt caagtctcaa ggggcaaacg
     3361 atatgcttcg tattcaagga aacaaagaaa aagttagtct cactcagaaa ttaactggct
     3421 tgaaaccaaa taccaagtat gccgtttatg tcggtgtcga taaccgtagt aatgccaagg
     3481 caagtatcac tgtgaatact ggtgaaaaag aagtgactac ttataccaat aagtctctcg
     3541 cgctcaacta tgttaaggcc tacgcccaca atacacgtcg tgataatgct acagttgatg
     3601 atacaagtta cttccaaaac atgtacgcct tctttacaac tggatcggac gtctcaaatg
     3661 ttactctgac attgagtcgt gaagctggtg atcaagcaac ttactttgat gaaattcgta
     3721 cctttgaaaa caattcaagc atgtacggag acaagcatga tacaggtaaa ggcaccttca
     3781 agcaagactt tgaaaatgtt gctcagggta tcttcccatt tgtagtgggt ggtgtcgaag
     3841 gtgttgaaga taaccgcact cacttgtctg aaaaacacga tccatataca caacgtggtt
     3901 ggaatggtaa gaaagtcgat gatgttatcg aaggaaattg gtcactcaag acaaatggac
     3961 tagtgagccg tcgtaacttg gtttaccaaa ctattccgca aaacttccgt tttgaagcag
     4021 gtaagaccta ccgtgtaacc tttgaatacg aagcaggttc agacaatacc tatgcttttg
     4081 tagtcggtaa gggagaattc cagtcaggtc gtcgtggcac tcaagcaagc aacttggaaa
     4141 tgcatgaatt gccaaatact tggacagatt ctaagaaagc caagaaggca accttccttg
     4201 tgacaggtgc agaaacagga gatacttggg taggtatcta ctcaactgga aatgcaagta
     4261 atactcgtgg tgattctggt ggaaatgcca acttccgtgg ttataacgac ttcatgatgg
     4321 ataatcttca aatcgaagaa attaccctaa caggtaagat gttgacagaa aatgctctga
     4381 agaactactt gccaacggtt gccatgacta actacaccaa agagtctatg gatgctttga
     4441 aagaggcggt ctttaacctc agtcaggccg atgatgatat cagtgtggaa gaagcgcgtg
     4501 cagagattgc caagattgaa gccttgaaga atgctttggt tcagaagaaa acggctttgg
     4561 tagcagatga ctttgaaagt ttggatgcgc cagctcaacc aggtgaaggt cttgagaatg
     4621 cctttgatgg aaacttatct agtttatggc atacatcatg gggcggagga gatgtaggca
     4681 agcctgcaac catggtcttg aaagaaccaa ctgaaatcac tggacttcgt tatgttccac
     4741 gtggatcagg ttcaaatggt aacttgcgtg atgtgaaact tgttgtgaca gatgagtctg
     4801 gcaaggagca tacctttact acaactgatt ggccagataa caataagtca aaagatattg
     4861 actttggtaa gacaatcaag gctaagaaaa ttgtccttac tggtaccaag acatacggag
     4921 atggtggaga taaataccaa tctgcagcgg aacttatctt tactcgtcca caggtagcag
     4981 aaacacctct tgacttgtca ggctatgaag cagctttggc taaggctcag aaattaacag
     5041 acaaagacaa tcaagaggaa gtagctagcg ttcaggcaag catgaaatat gcgacggata
     5101 accatctctt gacggaaaga atggtggaat actttgcaga ttatctcaac caattaaaag
     5161 attctgctac gaaaccagat gctccaactg tagagaaacc tgagtttaaa cttagctctt
     5221 tagcttccga tcaaggtaag acgccagatt ataagcaaga aatagataga ccagaaacac
     5281 ctgaacaaat cttgccagca acaggtgaga gtcaatctga cacagccctc ttcctagcag
     5341 gtgttagcct agccctatct gctctctttg tagtaaaaac gaagaaagac tagtatttag
     5401 taaaacctct taacaagatt acggaagcag tctctatctt ttccaatgag gtttatagta
     5461 cagaaaaaat ctgagaagat gtcttctcag acttttgtta agcacataaa tacaatagtg
     5521 ctatgacaaa atcacccaga aaatctgggt gataaatgtt atggttgtgc tggttgagga
     5581 ttctgatttt gttgatcagg ggttgtattt gattgttgcg tattattgtt aggattggta
     5641 gtcgtactat tatttgtgct tggagtggtt gagctagact gtgaagttga actatctgat
     5701 gatgagcttg aactttcagt tgatgggggt tgttgtggag caggtgagct ccacgtagaa
     5761 cgagcaccat ttttaaatac gaattctcca tttctgtaga gcccctctgg tatattccaa
     5821 tcctctggat tgcttccttc agacaggtag gtcatcatag agcggtaaac tttggcagcg
     5881 actgtaaggc cattgcctac aagtggtgtc agacggttag aatagcctgt ccatacagcc
     5941 attgaatatt tacgcgtata gccagcaaat agttcgtcag gtgctacaaa ttgagaggtc
     6001 ttgatgtggt tttcaatttc ctcgtctgta tagttagagg ttcctgtttt accagcctga
     6061 gggagccaag caagataggc atttcgtcca gttccataac tcaagactgt tttcatcatg
     6121 tcggtcatca tataggctgt cgtttctttc atggcacgag ttccgacatt agagaactct
     6181 ttttcactcc catcactaaa gacgacttta tggatataca ttggtttata gtaagttcca
     6241 ccatttgcaa aggcagcgta agcagcagcc atcttttcac tacttgctcc atattttttg
     6301 tctgattcgg ttgtgttact tgaaatggca tttgagtagt gaatacttgg gtagtcgatt
     6361 ccgagaccat ttaggaaagt cttggcgcgg ttgagtccga ccttgtttag agtttccacg
     6421 gctgggacgt ttcgcgattg ttgcagggcg tattgcaagg tgatgttgcc aaagtagccc
     6481 ctatcccagt tataaacagg agtatttgtc ccagggtagt tatagggctc atcgtgaacg
     6541 atagtagcag ttgaatcgta gacaccgtac tccaaggcag gagcatagtc tgtgatcggt
     6601 ttcatagttg atccccagtc gcggtttgtt tctactgctt ggttaattcc gaaggaaaca
     6661 ttacttgact gatggcgtgc tcctagctgg gcaatgactt taccgttaga aacatcaaca
     6721 atggtagaag cgacttgcaa ttcatcgtct ggataggcaa cgtattcgtc tgtattgtaa
     6781 atatcccaca gatgtttttg agcttcttgg tctacatttg tgtagacatc catcccagtt
     6841 gtgagcaggt tatagcctgt ttcttcttcc acttgattga tgacttcctt gaggtaatta
     6901 tccatgtaag cagggtaatt acttgctgat ttgagacttt gtagtccatc agtaattggt
     6961 gtattgactg ctttctcata ctgttcagca gagatgtagc cttgattttt catttcagat
     7021 aagaccaagt ttcggcggtc ttgggctgct tctggatgtg aataggggtc atattggttt
     7081 ggtgcctgag gcattccagc cagcaaggct aactgaggta aacttaaatt attgaggtct
     7141 ttaccatagt agttttgagc tgctgtctgc attccatagt tcccattaga catgtagacc
     7201 ttatttatat agtaggtcaa gatttcttgc ttggttgctt tttgttctaa ctgaatcgct
     7261 aaccaagctt cctgagcctt acgagaaata gtctggtcgg aagtcgaagt tgaaaagtaa
     7321 gtcaacttaa tcaactgttg ggtgagagtt gatccacctt ggagggaatt gctttgcaga
     7381 ttgcgcaaga aagctcccag gatacggatg gtatcaatcc ccctgtggtc gaagaagcga
     7441 tggtcttcga tagaaacgat tgccttaacc aaatctgtgg gaatatcatt agcttgggca
     7501 ttgacgcggc gttcagaacc caagtcagca atgagttgat ttttattgtc gtagatttta
     7561 ctagaagttg ttgcaactag tttactctcg gataggctag gagccttgct aacgtagtag
     7621 aaaaaaactc ctccgcctaa gacaatggct gcgataacca agcttaagaa gctaatgctc
     7681 agatacttga ttaggcgcag aatcgttggt ttgttcatct tgttttacca cctaataaat
     7741 gttctttgat aacattgaga taaggaattt gagggaaggc accagccttg atttcatatc
     7801 catattctcg aatatattca agtggcattg atttttgtcc cttatcttga tgatagaagc
     7861 gaatcaaatc gaatgccggc aataagtagg tttcttgctg agaagaaaag tgaagaagga
     7921 caaagcagat tccttgttgg gcaaggactt gttccatatg ctgaatctga tgtggatgaa
     7981 aatttttcat cggaatcgca cgtttttgtt ttgtttcctt gacttcaaag tcgatgtaat
     8041 atccatgata aacgccggaa tagtccgtcg ttgaagcttg tcgaaaatag gcttcaacaa
     8101 tcttggcacg acttcgttgt ggatagtcca cttgtacgat ttgaatagga gttggtttct
     8161 tatgtataac agccaagccc tgagacaaat agtagtcgtt ggtagcattg atcatctttt
     8221 caaaagacat tcctcgattt gcgaaatttt tgggttgaga aagagatgtt tgtctttttt
     8281 gtgatgaaac tttatgtgga tagttgacca taattctcct tattggtaca ataacatcac
     8341 tttattatac cataatttgc acagaaaggg ttaaaaatgg ctacagcttt ggttttaggc
     8401 tattctgctt ttgatttggg tttattttct gataaggatc ctcgtcttaa attgattaaa
     8461 aaagcgatac gtaaagattt agaggctatg gcagcagatg gggtgtcttg gcttgtgttt
     8521 acagggagtt tgggttttga atattgggtt ttagaggttg ctcaggagat gaaaaccgaa
     8581 tacggtttcc agttggccac catttttgct tttgaaaccc atggggaaaa ttggaatgaa
     8641 ggcaatcaga tgaaactgag tcgctttaag caggtagact ttgtcaaata tgcctatcct
     8701 cgctatgaac acaaggggca gttaagagat tatcagcagt ttttgctgga aaacacgact
     8761 agttcctatc ttttttatga tgaagaaaat gaaacaaaac tagcttattt ttaccaaaag
     8821 atgaaaaatc aagaggacta ttttataaag agattaacat ttgatgaatt aaatgaactt
     8881 gctgaaaatt tttccgaaaa ttgaagcttt gaccttgatt tttgtttgcc tttttttata
     8941 taataatact agcaagcgag aatggagaga gacatggcaa gtattatttt ttcagcgaaa
     9001 gatatttttg aacaagagtt tggacgtgaa gtccgtggct ataataaagt agaagttgac
     9061 gagtttttag acgatgtcat caaggactat gaaacctatg ctgccttggt caagtcactt
     9121 cgtcaggaaa ttgcggattt gaaggaagaa ttaactcgta aaccgaaacc ttcaccagtt
     9181 caagcagaac cccttgaagc ggcaattaca agttctatga cgaattttga tattttgaaa
     9241 cgcctgaata gattggaaaa agaagttttt ggtaaacaaa ttttagataa ctcagatttt
     9301 taagtagtta tttgagatgt gcaatttttg gataatcgcg tgaggagaat tgcttctcat
     9361 gaggaaagtc catgctagca caggctgtga tgcctgtagt gtttgtgcta ggcgaaacca
     9421 taagcctagg gacgagaaat cgttacggca gttgaaatgg ctaagtcctt ggataggcca
     9481 gagtaggctt gaaagtgcca cagtgacgaa gtctttctgg aaacagagag agtggaacgc
     9541 ggtaaacccc tcaagctagc aacccaaatt ttggtcgggg catggagtac gcggaaacga
     9601 acgtagtatt ctgactgcta tcagctagag ctgttagtgg tagacagatg attatcgaag
     9661 gaagtggtcc tagtcacttc tggaacaaaa catggcttat agaaaattgc atataggttg
     9721 gggctgagaa attttctcaa cctcattttt taaagtggac atatagaaag gtcttgcaag
     9781 actgtaacat gaaaaaagaa tttaatttaa ttgcaactgt ggcagcaggg cttgaggctg
     9841 tcgttggtcg tgaagtgcga gagttgggct acgattgcca ggttgaaaat ggacgtgttc
     9901 gttttcaagg agacgtgaga gctattatcg aaaccaacct ttggcttcgg gcagcagatc
     9961 gtatcaaaat tatcgtagga acgttcccag ctaagacttt tgaagagcta tttcagggag
    10021 ttttcgcttt agattgggaa aattatttac cacttggagc tcggttcccg atttcaaaag
    10081 ctaaatgtgt taagtccaaa cttcacaatg agcccagtgt tcaggctatt tctaagaaag
    10141 ctgttgtcaa gaaattgcag aaacactatg ctcgcccaga aggggttcct ctgatggaga
    10201 atggcccaga gtttaagatt gaggtctcta ttctcaaaga tgtggcaact gtcatgattg
    10261 atacgaccgg gtctagcctc tttaaacgtg gttatcgtac cgaaaaaggt ggcgctccta
    10321 tcaaggaaaa tatggcagca gccattttac aactttctaa ctggtatcca gacaagcctt
    10381 tgattgatcc gacctgtggt tcggggactt tctgtattga ggcagttatg attgctagaa
    10441 agatggcgcc aggtcttcgt cgctcttttg catttgagga atggaactgg atcagcgatc
    10501 gcttgattca agaagtgcgt acagaagcgg ctaaaaaagt agaccgtgag cttgagctgg
    10561 atatcatggg ctgtgatatt gatgctcgca tggtggaaat tgctaaggcc aatgctcagg
    10621 cagctggtgt tgcaggagac attactttta agcagatgcg cgtgcaggat ttacgttccg
    10681 ataaaatcaa tggagtaatc atttccaatc cgccttatgg tgaacgtttg tcagatgatg
    10741 caggggtgac caagctctat gctgagatgg ggcaagtatt tgcaccgctg aaaacttgga
    10801 gcaaatttat cctgactagt gatgaagctt ttgaaagcaa gtatggtagc caagcagata
    10861 agaagcgtaa gttatacaac ggaaccttga aagtggatct atatcaatat tttggtcagc
    10921 gtgtcaaacg gcaagaggta aaatagaaag ggatactcat gagtaaaaaa agacgaaatc
    10981 gtcataaaaa agaagctcaa gaaccgcaat ttgattttga tgaagcaaaa gagctaacag
    11041 ttggtcaagc tattcgtaaa aatgaagaag tggaagcagg agtcttgcct gaggattcca
    11101 ttttggacaa gtatgttaag caacacagag atgaaattga ggcggataag tttgcgactc
    11161 gtcaatacaa aaaagaggag ttcgttgaaa ctcagagtct ggatgattta attcaagaga
    11221 tgcgtgaggc tgtagagaag tcagaagctt cttcggagga agttccatct tctgaagaca
    11281 tcttactacc cttgcctctg gacgatgagg agcaaggctt ggatcctcta ttgctagatg
    11341 atgaaaatcc aacagaaatg actgaagaag tggaagagga gcaaaacctt tctcgtctgg
    11401 atcaagagga ctcagaaaag aaaagtaaaa aaggctttat tttgaccgtt ttggcgcttg
    11461 tatcagtaat tatttgtgtc agtgcttatt atgtctaccg tcaagtggct cgttcgacta
    11521 aggaaattga aacttctcaa tcaactacag ccaatcaatc ggatgtggat gattttaata
    11581 cactttatga cgccttttac acagatagca ataaaacggc tttgaaaaat agccagtttg
    11641 ataaactgag tcaactcaag actttacttg ataagctgga aggtagtcgt gaacatacgc
    11701 ttgccaaatc taaatatgat agtctagtaa cgcaaatcaa ggccattcaa gatgtcaatg
    11761 cacaatttga aaaactagct attgtggatg gtgtgttgga taccaatgcc aaagccaaat
    11821 cggatgctaa atttacagat attaaaactg ggaatacgga gcttgataaa gtgctagata
    11881 aggctatcag tcttggtaag agccagcaaa caagtacttc tagctcaagt tcaagtcaaa
    11941 ctagcagctc aagttcaagt caagcaagct caaatacgac tagtaagcca aaaccaagta
    12001 gttcaaatga gactagaagt agtcgcagtg aagtcaatat gggtctctcg agtgcagggg
    12061 ttgctgttca aagaagtgcc agtcgtgttg cctataatca gtctgctatt gatgatagta
    12121 ataactctgc ctgggatttt gcggatggtg tcttggaaca aattctagcg acttcacgtt
    12181 cacgtggcta tatcactgga gaccaatata tccttgaacg tgtcaatatc gttaacggca
    12241 atggttatta caacctctac aagccagatg gaacctatct ctttaccctt aactgtaaga
    12301 caggctactt tgtcggaaat ggcgctggtc atgcggatga cttggattac taagcagtcg
    12361 ttacaaaatt ctttcttttc aaaagtaaaa atgataaaat aaaacaaatg aaacaagagg
    12421 agtgtcaaat gacaaaagct aactttggtg ttgtaggtat ggccgtaatg ggtcgtaacc
    12481 ttgcccttaa tattgaatca cgtggttaca cagttgctat ctacaaccgt agtaaagaaa
    12541 aaacggaaga tgtgattgct tgccatcctg aaaagaactt tgtaccaagc tatgacgttg
    12601 aaagttttgt aaactcaatc gaaaaacctc gtcgtatcat gctgatggtt caagctggac
    12661 ctggtacaga tgctactatc caagcccttc ttccgcacct tgacaagggt gatatcttga
    12721 ttgacggtgg aaatactttc tacaaagata ccatccgtcg taatgaagaa ttggcaaact
    12781 caggtatcaa ctttatcggt actggagttt ctggtggtga aaaaggtgcc cttgaaggtc
    12841 cttctatcat gcctggtgga caaaaagagg cctacgaatt ggttgcggat gttcttgaag
    12901 aagtctcagc taaagcacca gaagatggca aaccatgtgt gacttacatc ggccctgatg
    12961 gagctggtca ctatgtgaaa atggttcaca atggtattga gtacggtgat atgcaattga
    13021 tcgcagaaag ctatgacttg atgcaacact tgctaggcct ttctgcagag gatatggctg
    13081 aaatctttac tgagtggaac aagggtgaat tagacagcta tttgatcgaa atcacagctg
    13141 atatcttgag ccgtaaagac gatgaaggcc aagatggacc aatcgtagac tacatccttg
    13201 atgctgcagg taacaaggga actggtaaat ggactagcca atcatctctt gaccttggtg
    13261 taccattgtc actgattact gagtcagtgt ttgcacgcta catttcaact tacaaggaag
    13321 aacgtgtaca tgctagcaag gtgcttccaa aaccagctgc cttcaacttt gaaggagaca
    13381 aggctgaatt gattgaaaaa atccgtcaag ccctttactt ctcaaaaatc atttcatacg
    13441 cacaaggatt tgctcaattg cgtgtagcct ctaaagaaaa caactggaac ttgccatttg
    13501 cagatatcgc atctatctgg cgtgatggct gtatcatccg ttctcgtttc ttgcaaaaga
    13561 ttacagatgc ttacaaccgt gatgcagacc ttgccaacct tcttttggat gaatacttct
    13621 tggatgttac tgctaagtat caacaagcag tgcgtgatat cgtagctctt gcggttcaag
    13681 ctggtgtgcc agtgccaact ttctcagcag ctattactta ctttgatagc taccgttcag
    13741 ctgaccttcc agctaacttg atccaagcac aacgtgacta ctttggtgct cacacttacc
    13801 aacgtaaaga caaagaagga accttccact actcttggta tgacgaaaaa taagtaggtc
    13861 agccatgggg aaacggattt tattacttga gaaagaacga aatctagctc attttttaag
    13921 tttggaactc cagaaagagc agtatcgggt tgatctggta gaggaggggc aaaaagccct
    13981 ctccatggct cttcagacag actatgattt gattttattg aacgttaatc tgggagatat
    14041 gatggctcag gattttgcag aaaaattgag tcgaactaaa cctgcctcag tcatcatgat
    14101 tttagatcat tgggaagact tgcaagaaga gctggaagtt gttcagcgtt ttgcagtttc
    14161 atacatctat aagccagtcc ttatcgaaaa tctggtagcg cgtatttcgg cgatcttccg
    14221 aggtcgggac ttcattgatc aacactgcag tctgatgaaa gttccaagga cctaccgcaa
    14281 tcttaggata gatgttgaac atcacacggt ttatcgtggt gaagagatga ttgctctgac
    14341 ccgccgtgag tatgaccttt tggcgacact tatgggaagc aagaaagtat tgactcgtga
    14401 gcaattgttg gaaagtgttt ggaagtatga aagtgcgacc gagacaaata tcgtagatgt
    14461 ctatatccgc tatctacgga gcaagcttga tgttaaaggc caaaaaagct acattaaaac
    14521 cgtgcgtggt gttggttaca ccatgcaaga atagaaaagc agttgcagtt gtgtaactgc
    14581 ttttttgagg agtttttata tattgacatg cagtcgggtc tttgctacaa tcgttttagg
    14641 agggtatata gatgaaaatt ttgaaaaaaa tgatgcaagt tctactagca gtctttttct
    14701 ttggtttgct agctacaaat acggtatttg cgaataccac aggtggccga tttgttgata
    14761 aggataatag aaaatattat gtaaaagatg atcataaagc aatctattgg cataaaatag
    14821 acggtaaaac ctactatttt ggtgatggag gagagagggt tgtcggttgg caatacttag
    14881 aaattcctgg aacaggttat cgtgataatt tattcgataa ccaaccagtt aatgaaattg
    14941 gccttcagga gaagtggtac tattttggac aagatggtgc tttgttagaa caaacagata
    15001 aacaagtact agaggcaaaa acgtctgaaa atacaggaaa agtatacggt gaacaatatc
    15061 ctctatctgc tgaaaagaga acttattatt ttgataataa ttatgttgta aagacaggct
    15121 ggatttatga agacggcaat tggtattatt taaataagct aggaaatttt ggcgatgatt
    15181 cttacaatcc actaccaatt ggtgaagttg ctaagggttg gactcaagat tttcatgtta
    15241 ctattgacat tgatagaagc aaacctgctc catggtacta cctagatgct tccggtaaga
    15301 tgcttacaga ttggcaaaaa gtaaacggaa aatggtatta ttttggctcc tctggttcta
    15361 tggcaacagg ttggaaatat gtacgaggca aatggtatta cttagataat aaaaatggtg
    15421 atatgaagac tggttggtat caagatggtt caacttggta ctatttagat ccttctaatg
    15481 gagatatgaa aataggttgg acaaaagtaa atggaaaatg gtattatctc aattcaaatg
    15541 gagcaatggt tacaggtagt caaactatcg atggtaaagt ttataacttc gcttcatctg
    15601 gtgagtggat ttaatgttgg aggatatata aaatgaagct tttgaaaaaa actatgcaag
    15661 ctggactaac agtaattttc tttggtttgc tagctacgaa tacagtattt gcagataact
    15721 ctgaaggatg gcagtttgtc caagaaaacg gtagaaccta ttacaaaaag ggggacctca
    15781 aagaaaccta ctggctagtg attgatggta agtactatta ttttgattct ctatctggag
    15841 agatggttgt cggctggcaa tatatcccgt ttccatctaa aggtagtaca attggtcctt
    15901 acccaaatgg tatcagatta gaaggttttc caaagtcaga gtggtactac ttcgataaaa
    15961 atggagtgct acaagagttt gttggttgga aaacattaga gattaaaact aaagacagtg
    16021 ttggaagaaa gtacggggaa aaacgtgaag attcagaaga taaagaagag aagcgttatt
    16081 atacgaacta ttactttaat caaaatcatt ctttagagac aggttggctt tatgatcagt
    16141 ctaactggta ttatctagct aagacggaaa ttaatggaga aaactacctt ggtggtgaaa
    16201 gacgtgcggg gtggataaac gatgattcga cttggtacta tttagatcca acaactggta
    16261 ttatgcaaac aggttggcaa tatctaggta ataagtggta ctacctccgt tcctcaggag
    16321 caatggccac tggctggtat caggaaggta ccacttggta ttatttagac cagccaaatg
    16381 gcgatatgaa aacaggttgg caaaaccttg ggaacaaatg gtactatctc cgttcctcag
    16441 gagcaatggc aactggttgg tatcaagatg gttcaacttg gtactaccta aatgcaggta
    16501 atggagacat gaagacaggt tggttccagg tcaatggcaa ctggtactat gcttatagct
    16561 caggtgcttt ggcagtgaat acgaccgtag atggctattc tgtcaactat aatggcgaat
    16621 gggttcggta atgacagggc gcttataatt atattcccaa atcattcata cctctctcaa
    16681 ctagatgtaa cttacaaaac ccctgacctc atgagccact ttcttcctcc tcatgaggtc
    16741 agttttactt tctgctgttc cagtatcgtt tttcctcgct agatttcctc aaaagggcag
    16801 actcctccct tggtgcgtca cacgattttt tcatctcgac tgttctttaa tgcatcatta
    16861 acgacgcttt ttttctaggt ggttcataag gaacaggaag attcaggttg acttttctaa
    16921 tcctagaata aagtgctgaa aacaattcgg aataggcata gagactagac aatttgagga
    16981 gctgcttgcg tcctgttcga acacattttc c
//
LOCUS       DAVLVP010000035        16632 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.035, whole genome shotgun sequence.
ACCESSION   DAVLVP010000035 DAVLVP010000000
VERSION     DAVLVP010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16632)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16632)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16632
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.035"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..1083
                     /gene="lytB"
                     /locus_tag="VYN69_001115"
     CDS             <1..1083
                     /gene="lytB"
                     /locus_tag="VYN69_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV1533397.1"
                     /translation="YDSHSQAWYYFKSGGYMTANEWMWDKESWFYLKSDGKMAEKEWV
                     YDSHSQAWYYFKSGGYMAKNETVDGYQLGSDGKWLGGKATNENAAYYQVVPVTANVYD
                     SDGEKLSYISQGSVVWLDKDRKSDDKRLAITISGLSGYMKTEDLQALDASKDFIPYYE
                     SDGHRFYHYVAQNASIPVASHLSDMEVGKKYYSADGLHFDGFKLENPFLFKDLTEATN
                     YSAEELDKVFSLLNINNSLLENKGATFKEAEEHYHINALYLLAHSALESNWGRSKIAK
                     DKNNFFGITAYDTTPYLSAKTFDDVDKGILGATKWIKENYIDRGRTFLGNKASGMNVE
                     YASDPYWGEKIASVMMKINEKLGGKD"
     gene            complement(1144..2799)
                     /gene="pavA"
                     /locus_tag="VYN69_001116"
     CDS             complement(1144..2799)
                     /gene="pavA"
                     /locus_tag="VYN69_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_094859469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rqc2 family fibronectin-binding protein PavA"
                     /protein_id="HEV1533398.1"
                     /translation="MSFDGFFLHHIVEELRSELVNGRIQKINQPFEQELVLQIRSNRQ
                     SHRLLLSAHPVFGRIQLTQTTFENPAQPSTFIMVLRKYLQGALIESIEQVENDRIVEI
                     TVSNKNEIGDHIQATLIIEIMGKHSNILLVDKSSHKILEVIKHVGFSQNSYRTLLPGS
                     TYIAPPSTKSLNPFTIKDEKLFEILQTQELTAKNLQSLFQGLGRDTANELERILVSEK
                     LSAFRNFFNQETKPCLTETSFSPVPFANQVGEPFANLSDLLDTYYKDKAERDRVKQQA
                     SELIRRVENELQKNRHKLKKQEKELLATDNAEEFRQKGELLTTFLHQVPNDQDQVILD
                     NYYTNQPIMIALDKALTPNQNAQRYFKRYQKLKEAVKYLTDLIEETKATILYLESVET
                     VLNQAGLEEIAEIREELIQTGFIRRRQREKIQKRKKLEQYLASDGKTIIYVGRNNLQN
                     EELTFKMARKEELWFHAKDIPGSHVVISGNLDPSDAVKTDAAELAAYFSQGRLSNLVQ
                     VDMIEVKKLNKPTGGKPGFVTYTGQKTLRVTPDSKKIASMKKS"
     gene            2904..3401
                     /gene="ybeY"
                     /locus_tag="VYN69_001117"
     CDS             2904..3401
                     /gene="ybeY"
                     /locus_tag="VYN69_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018380869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEV1533399.1"
                     /translation="MYIEMVDETGQVSKEMLQQTQEILEFAAQKLGKEDKEMAVTFVT
                     NERSHELNLEYRDTDRPTDVISLEYKPELEIAFDEEDLLENPELAEMMSEFDAYIGEL
                     FISIDKAHEQAEEYGHSFEREMGFLAVHGFLHINGYDHYTPEEEAEMFGLQEEILTAY
                     GLTRQ"
     gene            3382..3777
                     /locus_tag="VYN69_001118"
     CDS             3382..3777
                     /locus_tag="VYN69_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000378166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEV1533400.1"
                     /translation="MDSQDNKRKWKNRDLISSLEFALTGIFTAIKEERNMRKHAVTAL
                     VVILAGFVFQVSRIEWLFLLLSIFLVVAFEIINSAIENVVDLASHYHFSMLAKNAKDM
                     AAGAVLVVSLFAALTGALIFLPRIWDLLF"
     gene            3794..4693
                     /gene="era"
                     /locus_tag="VYN69_001119"
     CDS             3794..4693
                     /gene="era"
                     /locus_tag="VYN69_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002935752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEV1533401.1"
                     /translation="MTFKSGFVAILGRPNVGKSTFLNHVMGQKIAIMSDKAQTTRNKI
                     MGIYTTDKEQIVFIDTPGIHKPKTALGDFMVESAYSTLREVDTVLFMVPADEARGKGD
                     DMIIERLKAAKVPVILVVNKIDKVHPDQLLSQIDDFRNQMDFKEIVPISALQGNNVSR
                     LVDILSENLDEGFQYFPSDQITDHPERFLVSEMVREKVLHLTREEIPHSVAVVVDSMK
                     RDEETDKVHIRATIMVERDSQKGIIIGKGGAMLKKIGSMARRDIELMLGDKVFLETWV
                     KVKKNWRDKKLDLADFGYNEREY"
     gene            4742..5566
                     /gene="mutM"
                     /locus_tag="VYN69_001120"
     CDS             4742..5566
                     /gene="mutM"
                     /locus_tag="VYN69_001120"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001114577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEV1533402.1"
                     /translation="MPELPEVETVCRGLEKLIIGKKISSIEIRYPKMIKTDLEEFQRE
                     LPSQIIESMGRRGKYLLFCLTDKVLISHLRMEGKYFYYPDQVPERKHAHVFFRFEDGG
                     TLVYEDVRKFGTMELLVPDLLDAYFISKKLGPEPSEQDFDLQVFQAALAKSKKPIKSH
                     LLDQTLVAGLGNIYVDEVLWRAQVHPARPSQTLTAEEATAIHDQTIAVLGQAVEKGGS
                     TIRTYTNAFGEDGSMQDFHQVYDKTGQECVRCGTIIEKIQLGGRGTHFCPNCQRRD"
     gene            5566..6171
                     /locus_tag="VYN69_001121"
     CDS             5566..6171
                     /locus_tag="VYN69_001121"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEV1533403.1"
                     /translation="MGKIIGITGGIASGKSTVTNFLRQQGFQAVDADAVVHQLQKPGG
                     RLFEALVQHFGQEIILENGELNRPLLASLIFSNPEEQKWSNQIQGEIIREELATLREQ
                     LAQTEEIFFMDIPLLFEQDYSDWFAETWLVYVDRDAQVERLMKRDQLSKDEAESRLAA
                     QWPLEKKKDLASQVLDNNGNQNQLLNQVHILLEGGRQDDRD"
     gene            6158..7357
                     /locus_tag="VYN69_001122"
     CDS             6158..7357
                     /locus_tag="VYN69_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter PmrA"
                     /protein_id="HEV1533404.1"
                     /translation="MTEINWKDNLRIAWFGNFLTGASISLVVPFMPIFVENLGVGSQQ
                     VAFYAGLAISVSAISAALFSPIWGILADKYGRKPMMIRAGLAMTITMGGLAFVPNIYW
                     LIFLRLLNGVFAGFVPNATALIASQVPKEKSGSALGTLSTGVVAGTLTGPFIGGFIAE
                     LFGIRTVFLLVGSFLFLAAILTICFIKEDFQPVAKEKAIPTKELFTSVKYPYLLLNLF
                     LTSFVIQFSAQSIGPILALYVRDLGQTENLLFVSGLIVSSMGFSSMMSAGVMGKLGDK
                     VGNHRLLVVAQFYSVIIYLLCANASSPLQLGLYRFLFGLGTGALIPGVNALLSKMTPK
                     AGISRVFAFNQVFFYLGGVVGPMAGSAVAGQFGYHAVFYATSLCVAFSCLFNLIQFRT
                     LLKVKEI"
     gene            7357..7506
                     /gene="rpmG"
                     /locus_tag="VYN69_001123"
     CDS             7357..7506
                     /gene="rpmG"
                     /locus_tag="VYN69_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1533405.1"
                     /translation="MRVKINLKCSSCGSINYLTSKNSKTHPDKIEVLKYCPKERKVTL
                     HLESK"
     gene            7546..7779
                     /gene="secG"
                     /locus_tag="VYN69_001124"
     CDS             7546..7779
                     /gene="secG"
                     /locus_tag="VYN69_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEV1533406.1"
                     /translation="MYNLLLTILLVLSVVIVIAIFMQPTKNQSSNVFDASSGDLFERS
                     KARGFEAVMQRLTGILVFFWLAIALALTVLSSR"
     gene            7881..10235
                     /gene="rnr"
                     /locus_tag="VYN69_001125"
     CDS             7881..10235
                     /gene="rnr"
                     /locus_tag="VYN69_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000653285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEV1533407.1"
                     /translation="MKDRIKEYLQDKGKMTVNDLAQALGKDSSKDFRELIKTLSLMER
                     KHQIRFEEDGSLTLEIKKKHEISLKGIFHAHKNGFGFVSLEGEEDDLFVGKNDVNYAI
                     DGDTVEVVIKKVADRNKGTAAEAKIIDILEHSLTTVVGQIVLDQEKPKYAGYIRSKNQ
                     KISQPIYVKKPALKLEGTEVLKVFIDKYPSKKHDFFVASVLDVVGHSTDVGIDVLEVL
                     ESMDIVSEFPEAVVKEAESVPDAPSQKDMEGRLDLRDEITFTIDGADAKDLDDAVHIK
                     ALKNGNLELGVHIADVSYYVTEGSALDKEALNRATSVYVTDRVVPMLPERLSNGICSL
                     NPQVDRLTQSAIMEIDKHGRVVNYTITQTVIKTSFRMTYSDVNDILAGDEEKRKEYHK
                     IVPSIELMAKLHETLENMRVKRGALNFDTNEAKILVDKQGKPVDIVLRQRGIAERMIE
                     SFMLMANETVAEHFSKLDLPFIYRIHEEPKAEKVQKFIDYASSFGLRIYGTASEISQE
                     ALQDIMRAVEGEPYADVLSMMLLRSMQQARYSEHNHGHYGLAADYYTHFTSPIRRYPD
                     LLVHRMIRDYGRSKEIAEHFEQVIPEIATQSSNRERRAIEAEREVEAMKKAEYMEEYV
                     GEEYDAVVSSIVKFGLFVELPNTVEGLIHITNLPEFYHFNERDLTLRGEKSGITFRVG
                     QQIRIRVERADKMTGEIDFSFVPSEFDVIEKGLKQSSRSGRGRGSNRRSDKKEDKRKS
                     GRSNDKRKHSQKDKKKKGKKSFYKEVAKKGAKHGKGRGKGRRTK"
     gene            10198..10665
                     /gene="smpB"
                     /locus_tag="VYN69_001126"
     CDS             10198..10665
                     /gene="smpB"
                     /locus_tag="VYN69_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEV1533408.1"
                     /translation="MAKGEGKVVAQNKKARHDYTIVDTLEAGMVLTGTEIKSVRAARI
                     NLKDGFAQVKNGEVWLSNVHIAPYEEGNIWNQEPERRRKLLLHKKQIQKLEQETKGTG
                     MTLVPLKVYIKDGYAKLLLGLAKGKHDYDKRESIKRREQNRDIARVMKAVNQR"
     gene            10681..11542
                     /gene="tehB"
                     /locus_tag="VYN69_001127"
                     /pseudo
     CDS             10681..11542
                     /gene="tehB"
                     /locus_tag="VYN69_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase TehB"
     gene            11615..12568
                     /gene="coiA"
                     /locus_tag="VYN69_001128"
     CDS             11615..12568
                     /gene="coiA"
                     /locus_tag="VYN69_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000495911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEV1533409.1"
                     /translation="MFVARDARGELVNVLEDKLEKQAYTCPACGGQIHLRQGPSVRTH
                     FAHKSLKDCDFFFENESPEHLANKESLYHWLKKETKVQLEYPLSELKQIADVFVNGNL
                     ALEVQCSPLPQKVLKERSEGYRSQGYQVLWLLGQKLWLKERLSRLQQGFLYFSQNMGF
                     YVWELDKKKQVLRLKYLIYQDLRGKLHYQIKEFSYGQGSLLEILRLPYKRQKISHFTV
                     SEDKDICRYIRQQLYYQNLFWMKEQAEAYQKGENILTYGLKEWYPQIRPIVGKFFQIE
                     QDLTSYYRNFYTYYQKNPQNDWQKLYPPAFYQQYFLKNMVE"
     gene            12587..14389
                     /gene="pepF"
                     /locus_tag="VYN69_001129"
     CDS             12587..14389
                     /gene="pepF"
                     /locus_tag="VYN69_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEV1533410.1"
                     /translation="MVLQRNEINEKDTWDLSTIYPTDQAWEEALKDLTEQLETVAQYE
                     GHLLDSADNLLEITEFSLEMERQIEKLYVYAHMKNDQDTREAKYQEYYAKAMTLYSQL
                     DQAFSFYEPEFMEISEKQYADFLEAQPKLQVYQHYFDKLLQGKDHVLSQREEELLAGA
                     GEIFGSASETFAILDNADIVFPYVLDDDGKEVQLSHGTYTRLMESKKREVRRGAYQAL
                     YATYEQFQHTYAKTLQTNVKVQNYRAKVRNYKSARHAALAANFVPESVYDNLVAAVRK
                     HLPLLHRYLELRSKILGISDLKMYDVYTPLSSVEYSFTYQEALKKAEDALAVLGEDYL
                     SRVKRAFSERWIDVYENQGKRSGAYSGGSYDTNAFMLLNWQDNLDNLFTLVHETGHSM
                     HSSYTRETQPYVYGDYSIFLAEIASTTNENILTEKLLEEVEDDATRFAILNNFLDGFR
                     GTVFRQTQFAEFEHAIHQADQNGEVLTSDFLNKLYADLNQEYYGLSKEDNPEIQYEWA
                     RIPHLYYNYYVYQYSTGFAAASALAEKIVHGSQEDRDRYIDYLKAGKSDYPLNVMRKA
                     GVDMEKEDYLNDAFAVFERRLNEFEALVEKLGLA"
     gene            14391..15104
                     /locus_tag="VYN69_001130"
     CDS             14391..15104
                     /locus_tag="VYN69_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEV1533411.1"
                     /translation="MVESYSKNANHNMRRPVVKEEIVDLMRQRQKQVTGSLKELEDFA
                     RKENIPIIPHETVAYFRFLMETMQPKNILEIGTAIGFSALLMAEHAPNAKITTIDRNP
                     EMIGFAKENFAQFDSRKQITLLEGDAVDVLSTLTESYDFVFMDSAKSKYIVFLPEILK
                     HLEVGGVVVLDDIFQGGDVAKDIMEVRRGQRTIYRGLQKLFDATLDNPELTATLVPLG
                     DGILMLRKNVADVQLSESE"
     gene            15171..16112
                     /gene="prsA"
                     /locus_tag="VYN69_001131"
     CDS             15171..16112
                     /gene="prsA"
                     /locus_tag="VYN69_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PrsA"
                     /protein_id="HEV1533412.1"
                     /translation="MKKKLLAGAITLLSVATLAACSKGSEGADLISMKGDVITEHQFY
                     EQVKNNPSAQQVLLNMTIQKVFEKQYGSELDDKEVDDTIAEEKKQYGENYQRVLSQAG
                     MTLETRKAQIRTSKLVELAVKKVAEAELTDEAYKKAFDEYTPDVTAQIIRLNNEDKAK
                     EVLEKAKAEGADFAQLAKDNSTDEKTKENGGEITFDSASTEVPEQVKKAAFALDVDGV
                     SDVITATGTQAYSSQYYIVKLTKKTEKSSNIDDYKEKLKTVILTQKQNDSTFVQSIIG
                     KELQAANIKVKDQAFQNIFTQYIGGGDSSSSSSTSNE"
     gene            complement(16229..16570)
                     /locus_tag="VYN69_001132"
                     /pseudo
     CDS             complement(16229..16570)
                     /locus_tag="VYN69_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         5001 a         3103 c         3733 g         4795 t
ORIGIN      
        1 tacgattctc atagtcaagc ttggtactac ttcaaatccg gtggttacat gacagccaat
       61 gaatggatgt gggataagga atcttggttt tacctcaagt ctgatggcaa aatggctgaa
      121 aaagaatggg tctatgattc tcatagtcaa gcctggtact acttcaaatc tggtggctac
      181 atggcgaaaa atgagacagt agatggttat cagcttggaa gcgatggtaa atggcttgga
      241 ggaaaagcta caaatgaaaa tgctgcttac tatcaagtag tgcctgttac agccaatgtt
      301 tatgattcag atggtgaaaa gctttcctat atatcgcaag gtagtgtcgt atggctagat
      361 aaggatagaa aaagtgatga caagcgcttg gctattacta tttctggttt gtcaggctat
      421 atgaaaacag aagatttaca agcgctagat gctagtaagg actttatccc ttattatgag
      481 agtgatggcc accgttttta tcactatgtg gctcagaatg ctagtatccc agtagcttct
      541 catctttctg atatggaagt aggcaagaaa tattattcgg cagatggcct gcattttgat
      601 ggttttaagc ttgagaatcc cttccttttc aaagatttaa cagaggctac aaactacagt
      661 gctgaagaat tggataaggt atttagtttg ctaaacatta acaatagcct tttggagaac
      721 aagggcgcta cttttaagga agccgaagaa cattaccata tcaatgctct ttatctcctt
      781 gcccatagtg ccctagaaag taactgggga agaagtaaaa ttgccaaaga taagaataat
      841 ttctttggca ttacagccta tgatacgacc ccttaccttt ctgctaagac atttgatgat
      901 gtggataagg gaattttagg tgcaaccaag tggattaagg aaaattatat cgatagggga
      961 agaactttcc ttggaaacaa ggcttctggt atgaatgtgg aatatgcttc agacccttat
     1021 tggggcgaaa aaattgctag tgtgatgatg aaaatcaatg agaagctagg tggcaaagat
     1081 tagtactata agtgaatatg atttgagtga atagtaagtt aaaaatcctg atttcaagta
     1141 aaatcaggat tttttcatgg atgcaatttt tttggagtct ggtgtgacgc ggagggtctt
     1201 ttgtcctgtg taagtgacaa agccgggttt tccaccagtt ggtttattga gttttttgac
     1261 ttcaatcata tctacctgca ccagattcga caggcgccct tgagagaagt aggcagctaa
     1321 ctctgctgcg tctgtcttga ctgcatcaga tgggtcaaga tttcctgaga tgacaacatg
     1381 gcttccagga atgtccttag catggaacca aagttcctcc ttgcgggcca ttttaaaggt
     1441 caattcctca ttttgaagat tgtttcgtcc gacatagatg atggttttgc catcgcttgc
     1501 tagatattgt tctagttttt tgcgtttctg gattttctcc cgttgtcttc tgcggataaa
     1561 acctgtttga atcaattctt cacggatttc agcgatttct tccagtccag cttggttgag
     1621 gacggtttct acactttcca gatagagaat agtggctttg gtttcttcaa tcaaatcagt
     1681 caagtatttg acagcttctt tgagtttctg ataccgttta aaatagcgtt gggcattctg
     1741 gttgggagtc agagccttat caagcgcaat catgataggt tggttggtat agtagttgtc
     1801 taggataacc tggtcttggt cgttaggcac ttggtggagg aaggttgtca gcaattctcc
     1861 tttttgacga aattcttcag cgttgtctgt cgccagtaac tctttttcct gttttttgag
     1921 tttgtgtcgg tttttctgaa gttcattttc aacacgacga atcagttcac tggcctgctg
     1981 tttgacgcgg tcgcgctcag ccttatcctt atagtaggtg tccaacaaat cagaaagatt
     2041 tgcaaaaggc tctcccacct gatttgcaaa aggaactgga ctgaaggaag tctcagtcaa
     2101 gcatggcttg gtttcttgat tgaaaaaatt tcggaaagcg gaaagttttt cactaaccag
     2161 tatcctttcc aattcatttg ccgtatcgcg tcccagacct tgaaagaggc tttgaagatt
     2221 ttttgctgtt agttcttggg tttgcaggat ttcaaagagc ttttcatcct tgatagtaaa
     2281 aggattgaga gattttgtac ttggcggagc gatataggtc gatcctggaa gtaaggtgcg
     2341 gtagctattt tgtgaaaagc cgacgtgttt gataacttcg aggattttat gactgctttt
     2401 atcgaccagt agaatattac tgtgtttccc cataatttcg ataatcaagg tagcctggat
     2461 atggtctcca atctcgtttt tattggaaac tgtaatttcc acaatacggt cattttccac
     2521 ttgctcaatc gactcaatca gggccccctg caaatacttt ctcaaaacca tgataaaggt
     2581 agaaggttgg gctggatttt caaaagtcgt ttgggtcagc tgaatgcgtc caaaaactgg
     2641 atgggcagaa aggagcaggc gatggctttg gcgattgctg cggatttgca agaccaactc
     2701 ttgttcaaaa ggctgattga ttttctggat gcgaccattc actaattcgc ttcgcaattc
     2761 ctcaactatg tggtgtaaaa aaaatccgtc aaatgacatc gttctctcct tgtgattgta
     2821 ttccatagta ttatatcaaa aaggtagaat aaaatcatgg aaatgtggta taataaagcc
     2881 aagtaaagag aaacgagaag cacatgtata ttgaaatggt agatgaaact ggtcaagttt
     2941 caaaagaaat gttgcaacaa acccaagaaa ttttggaatt tgcagcccaa aaattaggaa
     3001 aagaagacaa ggagatggca gtcacttttg tgaccaatga gcgtagtcat gaacttaatc
     3061 tggagtaccg tgacaccgac cgtccgacag atgtcatcag ccttgagtat aaaccagaat
     3121 tggaaattgc ctttgacgaa gaggatttgc ttgaaaatcc agaattggca gagatgatgt
     3181 ctgagtttga tgcctatatt ggggaattgt tcatctctat cgataaggct catgagcagg
     3241 ccgaagaata tggtcacagc tttgagcgtg agatgggctt cttggcagta cacggctttt
     3301 tacatattaa cggctatgat cactacactc cggaagaaga agcggagatg ttcggtttac
     3361 aagaagaaat tttgacagcc tatggactca caagacaata aacgaaaatg gaaaaatcgt
     3421 gacttgatat ccagtttaga atttgctttg acaggtattt ttactgctat caaggaagaa
     3481 cgcaatatgc gaaaacacgc agtgacggct ctagtggtca tccttgcagg ttttgttttt
     3541 caggtgtcac gaatcgaatg gctctttctc ctattgagta ttttcttggt agtagccttt
     3601 gagattatca actctgctat tgaaaatgtg gtggatttgg ccagtcacta tcacttttcc
     3661 atgctggcta aaaatgccaa ggatatggcg gccggcgcgg tattagtggt ttctcttttc
     3721 gcagccttaa caggcgcatt gatttttctc ccacgaatct gggatttatt attttaaaca
     3781 gtaagaggaa attatgactt ttaaatcagg ctttgtagcc attttaggac gtcccaatgt
     3841 tgggaagtca acctttttaa atcacgttat ggggcaaaag attgccatca tgagtgacaa
     3901 ggcgcagaca acgcgcaata aaatcatggg aatttacacg actgataagg agcaaattgt
     3961 ctttatcgac acaccaggga ttcacaagcc taaaacagct ctcggagatt tcatggttga
     4021 gtctgcctac agtacccttc gcgaagtgga cactgttctt ttcatggtgc ctgctgatga
     4081 agcgcgtggt aagggggacg atatgattat cgagcgtctc aaggctgcca aggttcctgt
     4141 gattttggtg gtgaataaaa tcgataaggt tcatccagac cagctcttgt ctcagattga
     4201 tgacttccgt aatcaaatgg actttaagga aattgttcca atctcagccc ttcagggaaa
     4261 taacgtgtct cgtctagtgg atattttgag tgaaaatctg gatgaaggtt tccaatattt
     4321 cccgtctgat caaatcacag accatccaga acgtttctta gtttcagaaa tggttcgcga
     4381 gaaagtcttg cacctaactc gtgaagagat tccgcattct gtagcagtag ttgttgactc
     4441 tatgaaacga gacgaagaga cagacaaggt tcacatccgt gcaaccatca tggtcgagcg
     4501 cgatagccaa aaagggatta tcatcggtaa aggtggcgct atgcttaaga aaatcggtag
     4561 catggcccgt cgtgatatcg aactcatgct aggagacaag gtcttcctag aaacctgggt
     4621 caaggtcaag aaaaactggc gcgataaaaa gctagatttg gctgactttg gctataatga
     4681 aagagaatac taagtagagg taggctcatg cctgcttctt gtttttacag aaggaggact
     4741 tatgcctgaa ttacctgagg ttgaaaccgt ttgtcgtggc ttagaaaaat tgattatagg
     4801 aaagaagatt tcgagtatag aaattcgcta ccccaagatg attaagacgg atttggaaga
     4861 gtttcaaagg gaattgccta gtcagattat cgagtcaatg ggacgtcgtg gaaaatattt
     4921 gcttttctgc ctgacagaca aggtcttgat ttcccatttg cggatggagg gcaagtattt
     4981 ttattatcca gaccaagtgc ctgaacgcaa gcatgcccat gttttcttcc ggtttgaaga
     5041 tgggggcacg cttgtttatg aggatgtacg caagtttgga accatggaac tcttggtgcc
     5101 tgacctttta gacgcctact ttatttctaa aaaattaggt cctgaaccaa gcgaacaaga
     5161 ctttgattta caggtctttc aagctgccct tgccaagtcc aaaaagccta tcaaatccca
     5221 tctcctagac cagaccttgg tagctggact tggcaatatc tatgtggatg aggttctctg
     5281 gcgagctcag gttcatccag ctagaccttc ccagactttg acagcagaag aagcgactgc
     5341 cattcatgac cagaccattg ctgttttggg ccaggctgtt gaaaaaggtg gctccaccat
     5401 tcggacttat accaatgcct ttggggaaga tggaagcatg caggattttc atcaggtcta
     5461 tgataagact ggtcaagaat gtgtacgctg tggtaccatc attgagaaaa ttcaactagg
     5521 cggacgtgga acccactttt gtccaaactg tcaaaggagg gactgatggg aaaaatcatc
     5581 ggaatcactg ggggaattgc ctcaggtaag tcaactgtga caaattttct aagacagcaa
     5641 ggctttcaag cagtggatgc cgacgcagtc gtccaccaac tacagaaacc tggtggtcgt
     5701 ctgtttgagg ctctagtaca gcactttggg caagaaatca ttcttgaaaa cggagaactc
     5761 aatcgccctc tcctagctag tctcatcttt tcaaatcctg aagagcaaaa atggtctaat
     5821 caaattcaag gggagattat ccgtgaggaa ctggctactt tgagagaaca gttggctcag
     5881 acagaagaga ttttcttcat ggatattccc ctactttttg agcaggacta cagcgattgg
     5941 tttgctgaga cttggttggt ctatgtggac cgagatgccc aagtagaacg cttaatgaaa
     6001 agggaccagt tgtccaaaga tgaagctgag tctcgtctgg cagcccagtg gcctttagaa
     6061 aaaaagaaag atttggccag ccaggttctt gataataatg gcaatcagaa ccagcttctt
     6121 aatcaagtgc atatccttct tgagggaggt aggcaagatg acagagatta actggaagga
     6181 taatctgcgt attgcctggt ttggtaattt tctgacagga gccagtattt ctttggttgt
     6241 accttttatg cccatcttcg tggaaaatct aggtgtaggg agtcagcaag tcgcttttta
     6301 tgcaggctta gcaatttctg tctctgctat ttccgcggcg ctcttttctc ctatttgggg
     6361 tattcttgct gacaaatacg gccgaaaacc catgatgatt cgggcaggtc ttgctatgac
     6421 tatcactatg ggaggcttgg cctttgtccc aaatatctat tggttaatct ttcttcgttt
     6481 actaaacggt gtatttgcag gttttgttcc taatgcaacg gcactgatag ccagtcaggt
     6541 tccaaaggag aaatcaggct ctgccttagg tactttgtct acaggcgtag ttgcaggtac
     6601 tctaactggt ccctttattg gtggctttat cgcagaatta tttggcattc gtacagtttt
     6661 cttactggtt ggtagttttc tatttttagc tgctattttg actatttgct ttatcaagga
     6721 agattttcaa ccagtagcca aggaaaaggc tattccaaca aaggaattat ttacctcggt
     6781 taaatatccc tatcttttgc tcaatctctt tttaaccagt tttgtcatcc aattttcagc
     6841 tcaatcgatt ggccctattt tggctcttta tgtacgcgac ttagggcaga cagagaatct
     6901 tctttttgtc tctggtttga ttgtgtccag tatgggcttt tccagcatga tgagtgcagg
     6961 agtcatgggc aagctaggtg acaaggtggg caatcatcgt ctcttggttg tcgcccagtt
     7021 ttattcagtc atcatctatc tcctctgtgc caatgcctct agcccccttc aactaggact
     7081 ctatcgtttc ctctttggat tgggaaccgg tgccttgatt cccggggtta atgccctact
     7141 cagcaaaatg actcccaaag ccggcatttc gagggtcttt gccttcaatc aggtattctt
     7201 ttatctggga ggtgttgttg gtcccatggc aggttctgca gtagcaggtc aatttggcta
     7261 ccatgctgtc ttttatgcga cgagcctttg tgttgccttt agttgtctct ttaacctgat
     7321 tcaatttcga acattattaa aagtaaagga aatctagtgc gagtaaaaat caatctcaaa
     7381 tgctcctctt gtggcagtat caattaccta accagtaaaa attcaaaaac ccatccagac
     7441 aagattgagg ttttaaagta ttgtcccaag gaaagaaaag taaccctaca tcttgaatct
     7501 aagtagattt tatggtaaaa taatagggat tttaaggagt ttgatatgta taacctatta
     7561 ttaaccattt tattagtatt atctgttgtg attgtgattg caattttcat gcaaccaacc
     7621 aaaaaccaat ccagcaatgt atttgatgcc agttcaggtg atttgtttga acgcagtaaa
     7681 gctcgcggtt ttgaagctgt aatgcagcgt ttgacaggga ttttagtctt tttctggcta
     7741 gccattgcct tagcattgac ggtattatca agtagataag aaaataatgg gcaggactag
     7801 gtctttgcct ctttttattt ttaaaggatg tttgagaagg ttttacagta aaagaaaatt
     7861 aaaaaatcta gaaagaaaat atgaaagata gaataaaaga atatttacaa gacaagggaa
     7921 agatgactgt taatgatttg gctcaggctt tgggaaaaga cagttccaag gattttcgtg
     7981 agttgattaa aaccttgtcc ttaatggaaa gaaagcacca aattcgtttt gaagaagatg
     8041 gtagtctgac attagaaatt aagaaaaaac atgagattag cctcaagggg atttttcatg
     8101 cccataaaaa tggctttggc tttgttagtc tggaaggcga ggaggacgac ctttttgtag
     8161 ggaaaaatga tgtcaactat gctattgatg gtgataccgt cgaggtagtg attaagaaag
     8221 tcgctgaccg caataaggga acagcagcag aagccaaaat tattgacatc ctagaacaca
     8281 gtttgacaac agttgtcggg caaatcgttc tggatcagga aaagcctaag tatgctggct
     8341 atattcgttc aaaaaatcag aaaatcagtc aaccgattta tgttaagaaa ccagccctaa
     8401 aattagaagg aacagaagtt ctcaaagtct ttatcgataa atacccaagc aagaaacatg
     8461 atttctttgt cgcgagtgtt ctcgatgtgg tgggacactc aacggatgtc ggaattgatg
     8521 ttcttgaggt tttggaatca atggacattg tatccgagtt tccagaagct gttgttaagg
     8581 aagcagaaag tgtgcctgat gctccgtctc aaaaggatat ggaaggtcgt ctggatctaa
     8641 gagatgaaat tacctttacc attgacggtg cggatgccaa ggacttggac gatgcagtgc
     8701 atatcaaggc tctgaaaaat ggcaatctgg agcttggggt tcacatcgca gatgtttcct
     8761 attatgtgac cgaggggtct gcccttgaca aggaagccct taaccgtgcg acttctgttt
     8821 acgtgacaga ccgagtggtg ccaatgcttc cagaacgact atcaaatggc atctgctctc
     8881 tcaatcccca agttgaccgc ctgacccagt ctgctattat ggagattgat aaacatggtc
     8941 gtgtggtcaa ctataccatt acacaaacag ttatcaagac cagttttcgt atgacctata
     9001 gcgatgtcaa tgatatccta gctggcgatg aagaaaagag aaaagaatat cataaaattg
     9061 taccaagtat cgaactcatg gccaagcttc atgaaacttt agaaaacatg cgtgtgaaac
     9121 gtggagctct caattttgat accaatgaag cgaagatttt agtggataaa caaggtaagc
     9181 ctgttgatat cgttcttcgg cagcgtggca ttgccgagcg gatgattgag tcttttatgt
     9241 tgatggctaa tgaaacagtt gccgaacatt tcagcaagtt ggatttgcct tttatctatc
     9301 gaattcacga ggagcctaag gctgaaaagg ttcagaagtt tattgattat gcttcgagtt
     9361 ttggcttgcg tatttatggg actgccagtg agattagtca ggaggcactt caagacatca
     9421 tgcgtgctgt tgagggagaa ccttatgcag atgtattgtc catgatgctt cttcgctcta
     9481 tgcagcaggc tcgttattcg gagcacaatc acggccacta tggactagct gctgactatt
     9541 atactcactt taccagtcca attcgtcgtt atccagacct tcttgttcac cgtatgattc
     9601 gggattacgg ccgttctaag gaaatagcag agcattttga acaagtgatt ccagagattg
     9661 cgacccagtc ttccaaccgt gaacgtcgtg ccatagaagc tgagcgtgaa gtcgaagcca
     9721 tgaaaaaggc tgagtatatg gaagaatacg tgggtgaaga gtatgatgca gttgtatcaa
     9781 gtattgtcaa attcggtctc tttgtcgaat tgccaaacac agttgaaggc ttgattcaca
     9841 tcactaatct acctgaattt tatcatttca atgagcgtga tttgactctt cgtggagaaa
     9901 aatcaggtat cactttccga gtgggtcagc agatccgtat ccgtgttgaa agagcggata
     9961 aaatgactgg agagattgat ttttcattcg tacctagtga gtttgatgtg attgaaaaag
    10021 gcttgaaaca gtctagtcgt agtggcagag ggcgtggttc aaatcgtcgt tcggataaga
    10081 aggaagacaa gagaaaatca ggacgctcaa atgataagcg taagcattca caaaaagaca
    10141 agaagaaaaa aggaaagaaa tctttttaca aggaagtagc taagaaagga gccaagcatg
    10201 gcaaagggcg agggaaaggt cgtcgcacaa aataaaaagg cacgccacga ctatacaatc
    10261 gtagatacgc tagaggcagg gatggtcctg actggaactg aaatcaagag tgtacgagct
    10321 gctcgaatta atctcaagga tggctttgct caagtgaaaa atggagaagt ttggctgagc
    10381 aatgttcata tcgcgcctta cgaagagggc aatatctgga accaggaacc agaacgtcgt
    10441 cgtaaactcc tgctccataa aaagcaaatt caaaaattgg aacaagagac caaagggaca
    10501 ggaatgacct tagttcccct taaggtctat ataaaagatg gctacgctaa gcttctttta
    10561 ggacttgcca aagggaagca tgactatgac aaacgggagt ctatcaaacg tcgtgagcaa
    10621 aatcgagata tcgcgcgtgt gatgaaagct gttaatcagc gataaaaaga ggaattgaaa
    10681 atggaaaaat tagttgccta taaacgcatg cctttgtgga ataaacaaac aatgcctgaa
    10741 gctgttcagc aaaagcacaa tacaaaagtt gggacttggg ggaaaattac tgtcttgaag
    10801 ggagctctca agtttattga attgacagaa gaaggggaag ttctagctga acacctcttt
    10861 gaagcagggg cagacaatcc aatggcccaa cctcaagcct ggcaccgagt ggaagctgcc
    10921 acagatgatg tggaatggta cttggaattt tattgtaaac ctgaggatta ttttgctaaa
    10981 aaatacaata ccaatcctgt tcattcagag gtcctagagg ccatgcagac agtgaaacaa
    11041 gggaaagctt tggatttggg ttgtggtcag gggcgtaatt ctctttttct agcccagcaa
    11101 gattttgatg tgacggctgt agatcaaaat tggactggct cttgaaatct tgcaaagcat
    11161 tgtggagcag gaagatttgg acatgcctgt tggcctttac gatatcaatt cagctagcat
    11221 tgaacaagaa tatgatttta tcgtttcaac agttgttctc atgtttctac aagcggaccg
    11281 cattccagct attattcaaa atatgcagga gaaaaccagt gttggtggtt acaaccttat
    11341 cgtttgtgcc atggacacgg aggattatcc ttgctcggtt aatttcccat tcacctttaa
    11401 agaaggagaa ctggcagact attacaagga ttgggaattg gttaagtaca atgaaaatcc
    11461 aggccatttg caccgtcgcg atgagaatgg caatcgtatt caactacgct ttgcgacctt
    11521 actagctaag aaaatcaagt aaacacacat gaagattagg aattttcctg atcttttttc
    11581 ttttttacga atgatataga aaaggaggga attcatgttt gttgcgagag atgctagggg
    11641 agaattggta aatgtgttag aggataaact tgagaagcaa gcatacacct gcccagcttg
    11701 tggaggccag atccatttgc gtcaaggacc aagtgtacgg acgcattttg cccataaatc
    11761 cttaaaagac tgtgattttt tctttgaaaa tgaaagtcca gaacacctgg ccaataagga
    11821 atccctctat cactggttga aaaaagagac aaaggttcaa ttagagtacc cgctttcaga
    11881 acttaaacag attgcggatg tatttgtaaa tggcaatcta gctctagaag ttcagtgtag
    11941 tcccttgcct cagaaagtcc ttaaagagcg aagtgagggc tatcgtagtc agggttacca
    12001 agtactgtgg ttgctgggtc aaaaactgtg gctcaaggag cgtttgagtc gtctacagca
    12061 aggttttctg tatttcagtc aaaacatggg cttttatgtt tgggaattag acaagaaaaa
    12121 acaagtttta agactcaaat acctgattta ccaggatctc cgcggtaaac tccattatca
    12181 aatcaaggaa ttttcctatg gtcaaggtag tttattggaa atattgcgtc ttccctataa
    12241 gagacaaaaa atatctcatt ttacagtttc tgaggacaag gacatctgtc gctatattcg
    12301 gcaacaactt tattatcaaa atctcttttg gatgaaagaa caagcagaag cctatcaaaa
    12361 gggagaaaat atcctgactt atggactgaa agaatggtat ccacaaattc gaccaatagt
    12421 gggcaaattt ttccagattg aacaagactt gactagctat tatcggaact tttataccta
    12481 ttaccaaaaa aatcctcaaa atgattggca aaagctttat ccaccagcct tttatcagca
    12541 atatttcttg aaaaatatgg tagaatagaa aggatggagg aatctaatgg tattacaaag
    12601 aaatgaaata aatgaaaaag atacatggga tctatcaacg atctacccaa ctgaccaggc
    12661 ttgggaagaa gccttaaaag atttaacaga acaattggag acagtagccc agtatgaagg
    12721 ccatctcttg gatagtgcgg ataacctatt agaaatcact gaattttctc ttgaaatgga
    12781 acgccagata gagaagcttt acgtttatgc tcatatgaag aatgaccagg atacacgtga
    12841 agctaagtat caagagtact atgccaaggc catgacactc tacagccagt tagaccaagc
    12901 cttttcattc tatgagcctg aatttatgga gattagcgaa aagcagtatg ctgacttttt
    12961 agaagctcaa ccaaagctgc aggtttatca acactatttt gacaagctct tgcaaggcaa
    13021 ggatcacgtt ctttcacaac gtgaagaaga attattggct ggagctggag aaatctttgg
    13081 ttcagcaagt gaaaccttcg ctatcttgga caatgcggat attgtgttcc cttatgtcct
    13141 agacgatgat ggtaaagaag ttcagctatc tcatgggact tacacacgtt tgatggagtc
    13201 taaaaaacgt gaggttcgcc gtggtgccta tcaagctctt tatgcgactt acgaacaatt
    13261 ccaacacacc tatgccaaaa ccttgcaaac caatgttaag gtgcaaaatt accgtgctaa
    13321 agttcgtaac tacaagagtg ctcgtcatgc agcccttgca gcgaattttg ttccagaaag
    13381 tgtttatgac aatttggtag cagcagttcg caagcatttg ccactcttac atcgctatct
    13441 tgagcttcgt tcaaaaatct tggggatttc agatctcaag atgtacgatg tctacacacc
    13501 gctttcatct gttgaataca gttttaccta ccaagaagcc ttgaaaaaag cagaagatgc
    13561 tttggcagtc ttgggtgagg attacttgag ccgtgttaaa cgtgccttca gcgagcgttg
    13621 gattgatgtt tacgaaaatc aaggcaagcg ttcaggtgcc tactctggtg gttcttacga
    13681 taccaatgcc tttatgcttc tcaactggca ggacaatctg gacaatctct ttactcttgt
    13741 tcatgaaaca ggtcacagta tgcattcaag ctatactcgt gaaactcagc cttatgttta
    13801 cggagattac tctatctttt tggctgagat tgcctcaact accaatgaaa atatcttgac
    13861 ggagaaatta ttggaagaag tggaagacga cgcaacacgc tttgctattc tcaataactt
    13921 cctagatggt ttccgtggaa cagttttccg ccaaactcaa tttgctgagt ttgaacacgc
    13981 cattcaccaa gcagatcaaa atggggaggt cttgacaagc gatttcctaa ataaactcta
    14041 cgcagacttg aaccaagagt attatggttt gagtaaggaa gacaatcctg aaatccaata
    14101 cgagtgggct cgtattccac acttatacta taactactat gtatatcaat attcaactgg
    14161 atttgcggcc gcctcagcct tggctgaaaa aattgtccat ggtagtcaag aagaccgtga
    14221 ccgctatatc gactacctca aggcaggtaa gtcggactat ccacttaatg tcatgagaaa
    14281 agctggtgtt gatatggaga aggaagacta cctcaacgat gcctttgcag tctttgaacg
    14341 ccgtttaaat gagtttgaag cccttgttga aaaattagga ttggcataaa atggttgaat
    14401 cgtatagtaa gaatgctaac cataacatgc gtcgtcctgt cgtcaaagaa gaaattgtag
    14461 acttgatgcg tcagcgtcaa aagcaagtca caggttcctt gaaagaattg gaagactttg
    14521 cccgcaagga aaatattcct attattcccc atgaaacggt tgcttatttc cgttttctta
    14581 tggaaaccat gcagcctaaa aatattctgg aaattgggac ggctatcggt ttttcagctc
    14641 tcttgatggc tgaacatgcg ccaaatgcta agattacaac tattgatcgt aatccagaaa
    14701 tgattggttt tgccaaggaa aattttgccc agtttgacag tcgcaagcaa atcactctcc
    14761 tagagggaga tgcggtggat gtcttatcta cactgacaga gtcttatgat ttcgtcttta
    14821 tggattctgc caagtctaaa tacatcgtct ttctgccaga aatcctcaaa catttggaag
    14881 ttggtggtgt ggttgtcttg gatgatattt ttcaaggtgg tgatgttgcc aaggatatta
    14941 tggaagtccg tcgtggtcag cgaaccattt atcgaggcct tcaaaaatta tttgatgcaa
    15001 ccttagacaa tccagaactc accgcaacat tagtgccttt aggagatggt attctcatgc
    15061 ttcgtaaaaa tgtagcagat gttcaactgt ctgaaagcga atgattttca gaaaaattta
    15121 agaaaaaata gtaaaataga tagagtaaca cttatctcaa aggagtagac atgaagaaaa
    15181 aattattggc aggtgccatc acactattat cagtagcaac tttagcagct tgttcgaaag
    15241 ggtcagaagg agcagacctt atcagcatga aaggggatgt catcacagaa catcaatttt
    15301 atgagcaagt gaaaaacaac ccttcagccc aacaagtctt gttaaatatg accatccaaa
    15361 aagtttttga aaaacaatat ggctcagagc ttgatgataa agaggttgat gatactattg
    15421 ccgaagaaaa aaaacaatat ggcgaaaact accaacgtgt cttgtcacaa gcaggtatga
    15481 ctcttgaaac acgtaaagct caaattcgta caagtaaatt agttgagttg gcagttaaga
    15541 aggtagcaga agctgaattg acagatgaag cctataagaa agcctttgat gagtacactc
    15601 cagatgtaac ggctcaaatc atccgtctta ataatgaaga taaggccaaa gaagttctcg
    15661 aaaaagccaa ggcagaaggt gctgattttg ctcaattagc caaagataat tcaactgatg
    15721 aaaaaacaaa agaaaatggt ggagaaatta cctttgattc tgcttcaaca gaagtacctg
    15781 agcaagtcaa aaaagccgct ttcgctttag atgtggatgg tgtttctgat gtgattacag
    15841 caactggcac acaagcctac agtagccaat attacattgt aaaactcact aagaaaacag
    15901 aaaaatcatc taatattgat gactacaaag aaaaattaaa aactgttatc ttgactcaaa
    15961 aacaaaatga ttcaacattt gttcaaagca ttatcggaaa agaattgcaa gcagccaata
    16021 tcaaggttaa ggaccaagcc ttccaaaata tctttaccca atatatcggt ggtggagatt
    16081 caagctcaag cagtagtaca tcaaacgaat agtccaaatc aatgaatcag ggaaaaaact
    16141 cgacttcagg aaaaaatgaa gcaaacattc ccacaataaa acgcgtagta caaggtttgt
    16201 actgatcccc aaaagttaga caaataattt atccgaagga tttagttctg tattgcacag
    16261 ggctaagtcc ttttagtttt accttaattc tcttgttgtt gtaataatca atatagtcta
    16321 taatggctcg ttccaattga ttaagtgatt taaatgtttg ctcatagcca taaaacattt
    16381 cggattttaa aatgccaaag aaagattcca tcctaccgtt gtcttggctg tttcctttgc
    16441 gtgacataga tgcttgaatt cccttactct ctaggaagcg atgataagaa tcgtgttgat
    16501 attgccagcc ttggtcacta tggagaatcg tattctcata gtgcttctct ttgaatgcct
    16561 gttccaacat taacgatcaa tcaatttaat catgtaccta agattagaat tgtttatctc
    16621 aaatttattt ga
//
LOCUS       DAVLVP010000036        16627 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.036, whole genome shotgun sequence.
ACCESSION   DAVLVP010000036 DAVLVP010000000
VERSION     DAVLVP010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16627
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.036"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..782)
                     /gene="lytB"
                     /locus_tag="VYN69_001133"
     CDS             complement(<1..782)
                     /gene="lytB"
                     /locus_tag="VYN69_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-beta-N-acetylglucosaminidase"
                     /protein_id="HEV1533413.1"
                     /translation="MNLGEFWYNKINKNRGRRLMKKVRFIFLALLFFLASPEGAMASD
                     GTWQGKQYLKEDGSQAANEWVFDTHYQSWFYIKADANYAENEWLKQGDDYFYLKSGGY
                     MAKSEWVEDKGAFYYLDQDGKMKRNAWVGTSYVGATGAKVIEDWVYDSQYDAWFYIKA
                     DGQHAEKEWLQIKGKDYYFKSGGYLLTSQWINQAYVNASGVKVQQGWLFDKQYQSWFY
                     IKENGNYADKEWIFENGHYYYLKSGGYMTANEWIWDKESWFYL"
     gene            complement(824..1762)
                     /locus_tag="VYN69_001134"
     CDS             complement(824..1762)
                     /locus_tag="VYN69_001134"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEV1533414.1"
                     /translation="MTTNRLQVSLPGLDLKNPIIPASGCFGFGQEYAKYYDLDLLGSI
                     MIKATTLEPRFGNPTPRVAETPAGMLNAIGLQNPGLEVVLAEKLPWLEREYPNLPIIA
                     NVAGFSKQEYAAVSHGISKATNVKAIELNISCPNVDHCNHGLLIGQDPDLAYDVVKAA
                     VEASEVPVYVKLTPSVTDIVTVAKAAEDAGASGLTMINTLVGMRFDLKTRKPILANGT
                     GGMSGPAVFPVALKLIRQVAQTTDLPIIGMGGVDSAEAALEMYLAGASAIGVGTANFT
                     NPYACPDIIENLPKVMDKYGISSLEELRQEVKESLR"
     gene            complement(1773..2573)
                     /locus_tag="VYN69_001135"
     CDS             complement(1773..2573)
                     /locus_tag="VYN69_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEV1533415.1"
                     /translation="MNLTCKKRLGVIRLETMKVVAQEEIAPAIFELVLEGEMIEAMRA
                     GQFLHLRVPDDAHLLRRPISISSIDKANKQCHLIYRIEGAGTAIFSTLSQGDTLDVMG
                     PQGNGFDLSDLDEQNQVLLVGGGIGVPPLLEVAKELHERGVKVVTVLGFANKDAVILK
                     TELAQYGQVFVTTDDGSYGIKGNVSVVINDLDSQFDAVYSCGAPGMMKYINQTFDDHP
                     RAYLSLESRMACGMGACYACVLKVPESETVSQRVCEDGPVFRTGTVVL"
     gene            complement(2823..3203)
                     /locus_tag="VYN69_001136"
     CDS             complement(2823..3203)
                     /locus_tag="VYN69_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1533416.1"
                     /translation="MASKMLHTCLRVENLEKSIAFYQDAFGFKELRRRDFPDHAFTIV
                     YLGLEGDDYELELTYNYDHGPYVVGDGFAHIALSTPDLEALHQEHSAKGYEVTEPNGL
                     PGTTPNYYFVKDPDGYKVEVIREK"
     gene            complement(3261..3620)
                     /gene="rplT"
                     /locus_tag="VYN69_001137"
     CDS             complement(3261..3620)
                     /gene="rplT"
                     /locus_tag="VYN69_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019786714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEV1533417.1"
                     /translation="MARVKGGVVSRKRRKRILKLAKGYYGAKHILFRTAKEQVMNSYY
                     YAYRDRRQKKRDFRKLWITRINAAARMNGLSYSQLMHGLKLAEIEVNRKMLADLAVND
                     AVAFTALADAAKAKLGK"
     gene            complement(3672..3872)
                     /gene="rpmI"
                     /locus_tag="VYN69_001138"
     CDS             complement(3672..3872)
                     /gene="rpmI"
                     /locus_tag="VYN69_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEV1533418.1"
                     /translation="MPKQKTHRASAKRFKRTGSGGLKRFRAYTSHRFHGKTKKQRRHL
                     RKASMVHSGDYKRIKAMLTRLK"
     gene            complement(3905..4435)
                     /gene="infC"
                     /locus_tag="VYN69_001139"
     CDS             complement(3905..4435)
                     /gene="infC"
                     /locus_tag="VYN69_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEV1533419.1"
                     /translation="MKTIAKQDLFINDEIRVREVRLIGLEGEQLGIKPLSEAQALADN
                     ANVDLVLIQPQAKPPVAKIMDYGKFKFEYQKKQKEQRKKQSVVTVKEVRLSPTIDKGD
                     FDTKLRNARKFLEKGNKVKVSIRFKGRMITHKEIGAKVLAEFAEATQDIAIIEQRAKM
                     DGRQMFMQLAPATDKK"
     misc_feature    complement(4451..4582)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(4742..5923)
                     /locus_tag="VYN69_001140"
     CDS             complement(4742..5923)
                     /locus_tag="VYN69_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533420.1"
                     /translation="MKDVSLFLLKKVFKSRLNWIILLLFASVLGVTFYLNSQTANSHS
                     LESRLESRIAANERAINENEEKLSQMSDTSSEEYQFAKNNLDVQKNLLTRKTEILTLL
                     KEGRWKEAYYLQWQDEEKNYEFVSNDPTASSGLKMGVDRERKIYQALYPLNIKAHTLE
                     FPTHGIDQIVWILEVIIPSLFVVAIIFMLTQLFAERYQNHLDTAHLYPVSKVTFAISS
                     LGVGVGYVTVLFIGICGFSFLVGSLISGFGQLDYPYPIYSLVNQEVTIGKIQDVLFPG
                     LLLAFLAFIVIVEVVYLIAYFFKQKMPVLFLSLIGIVGLLFGIQTIQPLQRIAHLIPF
                     TYLRSVEILSGRLSKQIDNVDLNWSMGMVLLPCLIILLLVGILFIERWGSSRKKEVFK
                     V"
     gene            complement(5927..6514)
                     /locus_tag="VYN69_001141"
     CDS             complement(5927..6514)
                     /locus_tag="VYN69_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000939891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533421.1"
                     /translation="MLNLTHVTLKTRQVILQDADFTFKKGRIYGLLAINGSGKTTLFR
                     AMSKLLPLSSGHIAVPPSLFYYESVEWLDGNLSGMDYLRLIKNIWKSDLNLRDEIAYW
                     EMADYISLPIRKYSLGMKQRLVIAMYFLSQAKCWLMDEITNGLDEYYRQKFFDRLAQI
                     DRQEQLVLLSSHYKEELVDICDRVVTIHQGQIEEV"
     gene            complement(6546..6734)
                     /locus_tag="VYN69_001142"
     CDS             complement(6546..6734)
                     /locus_tag="VYN69_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533422.1"
                     /translation="MTATKSYKYDWNTVLEYSTNYHDHQYAWIPEWSRYYSYSEYKVG
                     GGWNYARYEVINYYTGGY"
     gene            complement(6913..9153)
                     /locus_tag="VYN69_001143"
     CDS             complement(6913..9153)
                     /locus_tag="VYN69_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEV1533423.1"
                     /translation="MLQWIKNFSIPLIYLSFLLLWLYYAIFSASYLALLGFVFLLVCL
                     FIQFPWKSAGKVLIICGIFGFWFVFQNWQQSQASQNLADSVERVRILPDTVKVNGDSL
                     SFRGKADGRIFQVYYKLQSEEEKEAFQALTDLHEIGLEGKLSEPEGQRNFGGFNYQAY
                     LKTQEIYQTLNIKKIQSLQKIGSWDIGENLSSLRRKAVVWIKTHFPDPMRNYMTGLLL
                     GHLDTDFEEMNELYSSLGIIHLFALSGMQVGFFMNGFKKLLLRLGLTQEKLKWLTYPF
                     SLIYAGLTGFSASVIRSLLQKLLAQHGVKGLDNFALTVLVLFIVMPNFFLTAGGVLSC
                     AYAFILTMTSKEGEGLKAVASESLVISLGILPILSFYFAEFQPWSILLTFVFSFLFDL
                     TFLPLLSILFVLSFLYPVIQLNFIFEWLEGIIRLVSQVARRPLVFGQPNAWFLILLLI
                     SLALVYDLRKNIKKLTVLCLLITGLFLLTKHPLENEITMLDVGQGESIFLWDITGKTI
                     LIDVGGKAESYKKIEKWQEKMTTSNAQRTLIPYLKSRGVAKIDQLILTNTDKEHVGDL
                     SEVTKAFHVGEILVSKDSLKQKEFVAELQATQTKVRSMTVGENLPIFGSQLEVLSPRK
                     MGDGGHDDTLVLYGKFLDKQFLFTGNLEEKGEKDLLKQYPYLEVDVLKAAQHGSKKSS
                     SPAFLEKLKPELTLISVGKSNRMKLPHQETLTRLEGINSKVYRTDQQGAIRFKGLDSW
                     KIESVR"
     gene            complement(9137..9787)
                     /locus_tag="VYN69_001144"
     CDS             complement(9137..9787)
                     /locus_tag="VYN69_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEV1533424.1"
                     /translation="MEAIIEKIKEYKIIVICTGLGLLVGGFFLLKPAPQTPVKETNLQ
                     AEVAAVSKDLVSEKEVNKEEKEEPLEQDLITVDVKGAVKSPGIYDLPVGSRVNDAVQK
                     AGGLTEQADSKSLNLAQKVSDEALVYVPTKGEEAVSQQTGLGTASSISKEKKVNLNKA
                     SLEELKQVKGLGGKRAQDIIDHREANGKFKSVDELKQVSGIGAKTIEKLKDYVTVD"
     gene            complement(9855..10424)
                     /locus_tag="VYN69_001145"
     CDS             complement(9855..10424)
                     /locus_tag="VYN69_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1533425.1"
                     /translation="MESIFVKLAQYPSIETERLLLRPVTLDDAEAMFDYASDKGNTRY
                     TFPTNQSLEETKNNIAQFYLVNPLGRWGIELKSNGQFIGTIDLHKIDSVLKKAAIGYI
                     INKKYWNQGLTTETNRAVIELAFEKIGMNKLVALYDKANPASGKVMEKSGMRFSHAEP
                     YACMDQHEKGRIVTRVHYVLTKEDYFANK"
     gene            complement(10532..11536)
                     /locus_tag="VYN69_001146"
     CDS             complement(10532..11536)
                     /locus_tag="VYN69_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEV1533426.1"
                     /translation="MKEHSIDIQLSHPDDLFHLFGSNERHLRLMEEELDVVIHARTEI
                     VQVLGEESACEEARQVIQALMVLVNRGMTVGTPDVVTAISMVKNDEIDKFVALYEEEI
                     IKDNTGKPIRVKTLGQKLYVDSVKQHDVTFGIGPAGTGKTFLAVTLAVTALKRGQVKR
                     IILTRPAVEAGESLGFLPGDLKEKVDPYLRPVYDALYQILGKDQTTRLMEREIIEIAP
                     LAYMRGRTLDDAFVILDEAQNTTIMQMKMFLTRLGFHSKMIVNGDISQIDLPRNVKSG
                     LIDAQEKLKNIHQIDFVHFSAKDVVRHPVVAQIIRAYEPAPVKKEESEELESQPLSEG
                     "
     gene            complement(11622..11837)
                     /locus_tag="VYN69_001147"
     CDS             complement(11622..11837)
                     /locus_tag="VYN69_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002894860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEV1533427.1"
                     /translation="MRKSFYTWLMTERNPKSNSPKAILADLAFEEAAFPKHTDDFDEV
                     SRFLEEHASFSFNLGDFDSIWQEYLEH"
     gene            complement(11846..12700)
                     /gene="cvfB"
                     /locus_tag="VYN69_001148"
     CDS             complement(11846..12700)
                     /gene="cvfB"
                     /locus_tag="VYN69_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HEV1533428.1"
                     /translation="MNTNLASFIVGLIIDENDRFYFVQKDGQTYALAKEEGQHTVGDT
                     VKGFAYTDMKQKLRLTTLEVTATQDQFGWGRVTEVRKDLGVFVDTGLPDKEIVVSLDI
                     LPELKELWPKKGDQLYIRLEVDKKDRIWGLLAYQEDFQRLARPAYNNMQNQNWPAIVY
                     RLKLSGTFVYLPENNMLGFIHPSERYAEPRLGQVLDARVIGFREVDRTLNLSLKPRSF
                     EMLENDAQMILTYLESNGGFMTLNDKSSPDDIKATFGISKGQFKKALGGLMKAGKIKQ
                     DQFGTELI"
     gene            complement(12760..13317)
                     /gene="frr"
                     /locus_tag="VYN69_001149"
     CDS             complement(12760..13317)
                     /gene="frr"
                     /locus_tag="VYN69_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEV1533429.1"
                     /translation="MANVIIEKAKERMTQSHQSLAREFGGIRAGRANASLLDRVHVEY
                     YGVETPLNQIASITIPEARVLLVTPFDKSSLKDIERALNASDLGITPANDGSVIRLVI
                     PALTEETRRDLAKEVKKVGENAKVAIRNIRRDAMDEAKKQEKAKEITEDELKTLEKDI
                     QKVTDDAVKHIDDMTANKEKELLEV"
     gene            complement(13326..14063)
                     /locus_tag="VYN69_001150"
     CDS             complement(13326..14063)
                     /locus_tag="VYN69_001150"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000013002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEV1533430.1"
                     /translation="MANPKYKRILIKLSGEALAGERGVGIDIQTVQTIAKEIQEVHSL
                     GIEIALVIGGGNLWRGEPAAEAGMDRVQADYTGMLGTVMNALVMADSLQQVGVDTRVQ
                     TAIAMQQVAEPYVRGRALRHLEKGRIVIFGAGIGSPYFSTDTTAALRAAEIEADAILM
                     AKNGVDGVYNADPKKDKTAVKFEELTHRDVINKGLRIMDSTASTLSMDNDIDLVVFNM
                     NQSGNIKRVVFGENIGTTVSNNIEEKE"
     gene            complement(14147..15481)
                     /gene="trmFO"
                     /locus_tag="VYN69_001151"
     CDS             complement(14147..15481)
                     /gene="trmFO"
                     /locus_tag="VYN69_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000083696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HEV1533431.1"
                     /translation="MSQSYINVIGAGLAGSEAAYQIAERGIPVKLYEMRGVKSTPQHK
                     TDNFAELVCSNSLRGDALTNAVGLLKEEMRRLGSVILESAEATRVPAGGALAVDRDGF
                     SQMVTEKVANHPLIEVVRDEITELPTDVITVIATGPLTSDALAEKIHALNDGDGFYFY
                     DAAAPIIDVNTIDMSKVYLKSRYDKGEAAYLNAPMTKQEFMDFHEALVNAEEAPLNSF
                     EKEKYFEGCMPIEVMAKRGIKTMLYGPMKPVGLEYPDDYTGPRDGEFKTPYAVVQLRQ
                     DNAAGSLYNIVGFQTHLKWGEQKRVFQMIPGLENAEFVRYGVMHRNSYMDSPNLLEQT
                     YRSKKQPNLFFAGQMTGVEGYVESAASGLVAGINAARLFKGESEAIFPETTAIGSLAH
                     YITHADSKHFQPMNVNFGIIKELEGERIRDKKARYEKIAERALADLEEFLTV"
     gene            complement(15675..15917)
                     /locus_tag="VYN69_001152"
     CDS             complement(15675..15917)
                     /locus_tag="VYN69_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEV1533432.1"
                     /translation="MLHNFVSQGVYWKVRNIEAEAIRRNCERGAIFSGKIKHHEDSQF
                     KGDHYVECYAVLDNTVIARDRITVPIDPLCGKDFIE"
     gene            15983..16627
                     /locus_tag="VYN69_001153"
                     /pseudo
     CDS             15983..16627
                     /locus_tag="VYN69_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         4809 a         3755 c         3091 g         4972 t
ORIGIN      
        1 agataaaacc aagattcctt atcccaaatc cattcattgg ctgtcatgta gccaccggat
       61 tttagataat aatagtgacc attctcgaaa atccattctt tatcagcata gtttccattt
      121 tctttgatgt aaaaccaaga ttggtattgt ttgtcaaaaa gccaaccttg ctgtactttg
      181 acaccactag cattcacata agcttgatta atccactgac ttgtcagtag ataaccaccg
      241 gatttgaaat aataatcctt ccctttaatt tggagccatt ctttctctgc gtgctgtcca
      301 tctgctttga tataaaacca agcatcgtat tgagaatcat agacccagtc ttctattact
      361 ttggcacctg ttgcaccaac ataggaagtt cctacccaag catttctttt catctttcca
      421 tcttggtcaa gataataaaa ggctcccttg tcttctaccc attctgattt ggccatatag
      481 ccaccagatt tgaggtaaaa atagtcgtca ccttgcttta gccattcatt ttcagcatag
      541 ttagcatctg cttttatata gaaccaagat tgataatgag tatcaaaaac ccactcattc
      601 gctgcttgac tgccatcttc tttcagatac tgttttcctt gccaagtacc atcactagcc
      661 attgcaccct ctggactagc taagaaaaat agcagagcta aaaaaataaa tcttactttc
      721 ttcattaacc ttcttcctct gttcttattt attttattgt accaaaattc tcctaaattc
      781 atattacaaa ctaataaaaa atcaagaaca gattgattgc agtttacctc agagactctt
      841 ttacttcctg acggagttct tccagactgc taataccgta tttatccatg acttttggta
      901 aattttcgat gatgtcaggg caggcataag gattggtaaa gttagctgtt ccaactccga
      961 tagcagatgc cccagccaga tacatttcta gggcagcttc agccgaatcc actcccccca
     1021 ttccaatgat aggcaggtct gttgtttggg ctacttggcg gatgagtttg agggctactg
     1081 gaaagactgc tggacctgac attccacctg ttccattggc caagattggt tttctggttt
     1141 tgaggtcaaa gcgcattcca accagagtat tgatcatggt caagccactt gctcccgcat
     1201 cttctgcagc ttttgcgaca gtaacgatat cggtcacact cggggttaat ttgacataaa
     1261 ctggcacttc tgaggcttcc acagctgctt tcaccacatc ataagccaaa tctggatctt
     1321 gaccaatcaa aagtccatga ttacagtggt caacattggg acaagaaata ttgagctcga
     1381 tagcttttac attagttgcc ttggaaatcc catgagaaac agctgcatac tcttgttttg
     1441 aaaaaccagc tacattggca ataataggaa gatttggata ttctctttcc agccaaggta
     1501 gcttttcagc caaaacaacc tctaaaccag gattttgcaa gccaattgca ttgagcatac
     1561 cagcaggcgt ctctgccact cttggagttg gattcccaaa acgtggttca agggttgtcg
     1621 ccttgatcat aatagaacct aaaaggtcta aatcatagta cttggcatac tcttgtccaa
     1681 agccaaaaca gcctgatgct ggaataatcg gatttttcaa atccaaacca ggtagagaca
     1741 cttgtaatcg atttgtagtc ataattttct ccttataata caactgttcc tgtgcggaaa
     1801 acaggaccat cttcacagac gcgttggctg accgtctcgc tttctggtac ttttagaaca
     1861 caggcatagc aagctcccat cccacaagcc atacgagatt ccagagataa ataggctctt
     1921 gggtgatcat caaaggtttg attgatatac ttcatcattc ctggagcccc acacgagtaa
     1981 acagcatcaa actgactatc taaatcattg ataacaacgg aaacatttcc cttgatgcca
     2041 taagaaccat catctgtcgt tacaaagacc tgaccatact gagccaattc cgttttcaaa
     2101 ataacagcat ccttattagc aaaaccgagg actgtcacta ctttcactcc acgttcatgc
     2161 aattccttgg ccacctcaag caagggtgga acaccaatcc caccaccaac aaggagaacc
     2221 tgattctgct catcaaggtc agacaagtca aaaccatttc cctgaggccc catcacatca
     2281 agagtgtctc cctgacttaa ggttgaaaaa attgcagtcc cagctccctc aatccgataa
     2341 atgaggtgac actgcttgtt tgccttgtca atagacgaaa ttgaaatagg acgacgtaag
     2401 agatgggcat cgtctggtac acgcagatga agaaattggc ctgctcgcat ggcttcaatc
     2461 atttctcctt ctaggactaa ttcaaagatt gctggcgcga tttcctcttg tgcaaccacc
     2521 ttcatggttt ccaaccgaat gacacccaaa cgcttcttac atgtgagatt cataactttt
     2581 cctccttaaa ttttaagggg accagataaa gaaaagacct tgctagtgca aggcctcagt
     2641 taaaatcgta caacacaaga gagcacactc aaaaggtctc ctctagaaaa ttgcactatt
     2701 cttttatctg acaccttgtg agtctctctg gactcccctt aaaggttgaa tttttattag
     2761 tatactcttt cagctaaaaa aagtcaagta gaaaacgaat attctactta acttgcacga
     2821 aattattttt cacgaatgac ttcgacctta tatccatcag ggtctttaac aaagtaatag
     2881 tttggtgtag ttcctggtag accatttggc tcagtcactt catagccttt tgcactgtgt
     2941 tcttgatgaa gtgcctcaag atcaggtgta ctgagggcga tatgggcaaa cccatcacca
     3001 accacatacg gaccgtgatc gtagttataa gtcaactcca actcataatc atcaccctca
     3061 agacctagat agacaatcgt gaaggcatgg tctggaaaat ctctgcgacg caattcttta
     3121 aaaccaaaag catcttgata aaatgcgatt gatttttcaa gattttctac tcgtaagcaa
     3181 gtgtgtagca tttttgaagc catatctttc tcctttattt ttaaaaagac tggacaatcc
     3241 tgttccagtc tcatcagttg ttatttacca agttttgctt tagctgcatc tgcaagagct
     3301 gtgaaagcta ctgcatcgtt aacagccaag tcagcaagca ttttacggtt aacttcgatc
     3361 tcagccaatt tcaaaccatg catcaattgt gagtatgaaa gtccgttcat acgagccgcc
     3421 gcattgatac gagtaatcca caatttgcgg aagtcacgtt ttttctgacg acggtcacgg
     3481 tatgcatagt agtaagagtt cattacttgt tcttttgcag tacggaacaa gatgtgttta
     3541 gctccatagt aaccttttgc taatttaaga atacgtttac gacgtttgcg tgatacaacg
     3601 ccacctttaa cacgtgccat gttttatttc ctccaaatat tttctagaat tgtttactta
     3661 cagtcaagct attatttcaa gcgagtaagc attgctttga tacgtttgta atctcctgaa
     3721 tgcaccatag atgctttacg aagatgacga cgttgtttct tagtttttcc gtggaaacgg
     3781 tgagaagtgt aagcacggaa acgtttaagt ccaccagaac ctgtacgttt gaaacgttta
     3841 gctgatgcgc ggtgtgtttt ttgttttggc atgatttttt ctcctttatt taactttctg
     3901 acaattattt tttgtcagtc gctggcgcca attgcatgaa catttgacgt ccatccattt
     3961 tagcacgttg ttcgatgatg gcaatatctt gagttgcttc agcaaactcg gctaaaactt
     4021 ttgcaccaat ctctttatgg gtaatcatac gacccttaaa gcgaatagat accttaactt
     4081 tatttccttt ttcaaggaac ttacgtgcat tacgaagttt tgtatcaaag tcacccttgt
     4141 caattgttgg acttagacga acttctttca cagtaacaac gctttgtttt ttacgttgtt
     4201 ctttttgctt cttctggtac tcaaatttga acttaccgta gtccataatt tttgcaacag
     4261 gcggtttggc ttggggttga atcaatacta ggtcaacatt agcgttatca gccaaagctt
     4321 gcgcttcact gagtggcttg atgcctagct gttctccctc aagaccaatc aagcgaactt
     4381 cacgtacacg aatctcatca ttgatgaata agtcttgctt tgctatggtt ttcacctctt
     4441 ttgttttatt agagaaaaac aatagcggac tcgtaaatat acaagcccgc acgttatgat
     4501 agcgtttctt cgaaactttt catccgtagg gccaggcaac ttaatgtcac aaggcgagaa
     4561 gctctcactt ctgcttttct caacttttat atgataccaa gttttctagc tcttgtcaag
     4621 ataaaaagtt attttttgaa aagtttgtgt ttgtattttg gtttggtttt cgaatcaaaa
     4681 agagagaacc aagttgattc tctccctatg ttagtttttc tttagcttcc ctataggaaa
     4741 actaaacttt aaaaacttcc tttttacgtg aacttcccca tctttcgata aatagaatcc
     4801 ccactaacaa aaggataatc aggcaaggaa gtaagaccat ccccatgctc caatttagat
     4861 cgacattatc aatctgctta gataatcttc cagataaaat ctccactgaa cgcaagtaag
     4921 taaagggaat cagatgtgca atcctttgaa gaggctgaat tgtttggatg ccaaacaata
     4981 agccaacaat cccaatgagt gaaagaaaga ggacaggcat tttttgcttg aaaaagtaag
     5041 caatcaagta cacaacttcc acaatgacga taaaggctaa gaaagctaag agcaagccag
     5101 gaaataatac atcttgtatt ttcccaatag ttacttcttg attcactaag ctataaattg
     5161 ggtagggata atctaactgt ccaaaaccac ttatcagact tcccactaga aaagaaaagc
     5221 cacagattcc gataaacagc acagttacat atcccactcc aactccaaga gaggatattg
     5281 caaatgtcac ttttgaaaca ggatataagt gagctgtgtc cagatgattt tgatatcttt
     5341 ctgcaaatag ttgtgttagc ataaaaataa tcgcaaccac aaacaagctt gggatgataa
     5401 cctctaaaat ccagacaatc tgatcaatcc cgtgggtcgg aaactccaaa gtatgtgctt
     5461 ttatgttcaa gggatacagg gcttggtaaa tcttccgttc gcggtcaacc cccattttta
     5521 agccagagct agcagtcggg tcatttgata caaattcata attcttctct tcatcttgcc
     5581 actgcagata gtaggcttct ttccagcgcc cttcttttag taaagttaga atttctgtct
     5641 ttcgcgtcaa aagatttttt tgcacgtcta aattattttt agcaaactgg tattcctccg
     5701 agctggtatc agacatttgg gagagtttct cttcattttc attgatagcc ctctcgttgg
     5761 ctgcaatgcg actttccaac ctgctctcca agctgtgtga gtttgcagtc tgactattta
     5821 aataaaaggt aacaccgagt acagatgcaa ataaaagtaa gataatccag tttaaacgac
     5881 ttttgaaaac ttttttcaat aaaaatagac taacatcttt cataaactaa acctcttcta
     5941 tctgcccctg atgaatggtt actactctat cgcagatatc aaccaactct tccttatagt
     6001 gggaacttaa aagaaccagc tgttcttgtc tatcgatttg tgctagccta tcaaaaaact
     6061 tctgtcgata atactcgtct aagccatttg taatctcatc catgagccag catttggcct
     6121 gactgagaaa atacatagca atcaccaagc gttgcttcat gcctaaggaa tacttgcgga
     6181 tgggaagact gatatagtca gccatttccc agtaggcgat ttcatctctc aagtttaggt
     6241 ctgacttcca gatgtttttt atgagacgaa ggtagtccat cccacttaag tttccatcca
     6301 gccattcaac gctctcataa taaaacaaag aaggaggaac tgcgatgtgt ccactactaa
     6361 ggggaagcaa cttgctcata gctcggaata gtgtcgtctt tcccgagcca ttgatagcaa
     6421 gaaggccata aatcctaccc tttttaaagg taaaatccgc atcttgcaag atgacttgtc
     6481 gcgtttttaa ggtaacatga gtaagattta acatatccag ccctcctttt ctcactcttt
     6541 aaggattaat aacctccagt atagtagttt atgacctcat aacgagcgta gttccagcct
     6601 ccgccaactt tatactcaga atagctgtaa taacgagacc attccggaat ccaagcatac
     6661 tgatggtcgt gatagttggt actatattcc aaaaccgtat tccaatcata cttgtaactt
     6721 ttagtggctg tcacagcaga tacactggac tgaagaagac caatagatta taaactaact
     6781 aataaaacaa cttttgctga tttttaatga ttttatatcc tcaatattga atacgttttc
     6841 attataccat ttttatcatt attatgcaac aaatttttag tttatttcac taatctacaa
     6901 catttatcct tcctatcgaa cactttcgat tttccaacta tccaacccct taaaacgtat
     6961 agctccttgc tggtcagttc ggtaaacttt gctattgata ccttccagtc gtgtcaatgt
     7021 ttcctgatgg gggagtttca ttcgattgct ctttccaact gagataagag taagctccgg
     7081 tttgagtttt tctagaaagg ctggacttga tgacttttta gagccatgtt gggcagcttt
     7141 caatacatcc acctctaagt aaggatattg cttcagcaag tccttctctc ctttctcctc
     7201 caaatttccc gtgaagagaa attgcttatc caagaatttc ccatacagaa ctagggtatc
     7261 atcgtgtcct ccatctccca ttttccttgg agatagaact tctaactgac ttccaaaaat
     7321 gggcaagttc tcccctactg tcatactacg cacctttgtt tgagtcgcct gtagttctgc
     7381 cacaaattcc ttctgtttca gactgtcttt tgatactaga atctccccta catggaaagc
     7441 cttggtcacc tctgacaaat ctccaacatg ctccttgtcc gtgttagtca aaattagctg
     7501 gtcaatctta gctactcctc gacttttgag atagggaatc aaggttcgct gggcattgct
     7561 ggtcgtcatc ttttcttgcc atttttcgat tttcttataa gattctgcct tgccaccgac
     7621 atctatgaga atggttttcc cagttatatc ccataggaaa atactttctc cttgccccac
     7681 atccagcatg gtgatttcat tttccagtgg atgcttggtc aggagaaaga gccctgtaat
     7741 caataagcac aataccgtta gctttttaat gttttttctc aaatcataga ccaaagccaa
     7801 ggaaattaac aataggatta aaaaccatgc gttgggttga ccaaagacaa gcggtctcct
     7861 tgccacctgc gagaccaagc gaataatgcc ctctaaccat tcaaagataa agttcagctg
     7921 aatgactgga tagagaaagg aaaggacaaa taaaatagac aagagcggta agaaggtcaa
     7981 gtcaaataga aaggaaaaga caaaggtcaa aaggatagac caaggttgaa attccgcaaa
     8041 ataaaaggat agaatgggca atatgcccaa ggagatgact agactttcac tagcaacagc
     8101 cttgagcccc tccccttctt tgctggtcat ggtcaggata aaagcataag cgcaggacaa
     8161 gactcctcct gctgtcaaga aaaagtttgg catgacaata aagaggacaa gcaccgtcaa
     8221 ggcaaaatta tccaagccct taaccccatg ttgagccagt agcttttgca agagactgcg
     8281 aataaccgat gctgaaaatc cagttagtcc cgcatagata agggaaaagg gataagttag
     8341 ccatttcaac ttttcttggg tcaagcccaa tcgcaagaga agtttcttaa atccattcat
     8401 gaaaaaacct acctgcatgc cagatagggc aaagaggtgg ataattccta gactggaata
     8461 aagctcattc atctcctcaa agtcggtgtc cagatgtccc agcaaaagtc ctgtcatgta
     8521 attgcgcata gggtctggaa agtgcgtctt aatccaaacc acagcctttc gacgtaaact
     8581 ggacaagttt tctcctatat cccaactgcc aatcttttga agtgactgga tttttttgat
     8641 attgagagtc tggtaaattt cctgagtctt cagataggct tggtaattaa agccaccaaa
     8701 atttctctgc ccttctggct ccgaaagctt cccttctagt cctatctcat gcaggtcggt
     8761 taaagcttga aaggcttctt tctcctcctc ggactggagt ttataataga cttgaaaaat
     8821 gcgtccatca gccttgccgc gaaaggacag actatcacca ttgaccttaa cagtgtcagg
     8881 cagaatccgt accctttcaa cagaatccgc cagattttga ctcgcttgac tctgttgcca
     8941 attttgaaaa acaaaccaaa atccaaagat tccgcaaatt attagaactt taccagcaga
     9001 tttccacgga aattggataa agagacagac tagcagaaaa acaaagccca acaaagcaag
     9061 ataggatgct gagaaaatag cgtaataaag ccaaagtaat agaaaactca ggtaaattag
     9121 gggaatagag aaattcttaa tccactgtaa catagtcttt aagcttttct atagtcttgg
     9181 caccaatgcc agagacctgc ttgagctcgt ctactgactt gaacttgcca tttgcctcac
     9241 gatggtcgat aatgtcctga gctcgttttc ctcccagtcc cttgacctgc ttgagttctt
     9301 ccagactggc cttgttgaga ttgaccttct tttccttgct tattgaagaa gctgtcccca
     9361 aaccagtctg ttggctaact gcttcttctc ccttagtagg aacgtaaacc agagcctcat
     9421 cactaacttt ctgagctaga ttgagcgact tgctgtctgc ttgctctgtc aagccaccag
     9481 ccttctgaac agcatcattg actcgactac ctacaggcaa gtcataaatc cctggcgatt
     9541 tgacagcacc tttgacatct actgtgatta gatcttgttc aaggggttct tccttttctt
     9601 ccttgttcac ttccttttcg gataccaaat ccttggaaac agctgcgact tcagcctgca
     9661 aattcgtctc tttgacaggt gtttgtggag ctggttttag caggaaaaat cctcctacaa
     9721 gcaagcccag accagtacag atgacgatga ttttatactc tttgattttc tcgataattg
     9781 cttccatatt ttctcctctc ttagattatt cgtaagagga agaaaaaaca gtcgaaaaat
     9841 ttcttttcaa ctgcttattt atttgcaaaa tagtcttcct tggtcaagac ataatgaact
     9901 cttgtcacga ttcggccttt ttcatgctgg tccatacaag catatggttc tgcatgggaa
     9961 aaacgcattc ctgatttctc catgaccttt cctgatgcgg gattagcctt atcgtataaa
    10021 gcaactaact tattcattcc tatcttctca aaagctagct caatcacagc acgattggtt
    10081 tctgtcgtta atccttgatt ccaatacttt ttattgataa tgtagccaat agctgccttc
    10141 ttaagaacag aatcaatctt gtgcaagtca atggttccaa taaactgacc attgcttttt
    10201 agttctattc cccaacgtcc caagggatta accaaataga actgagcaat gttattcttg
    10261 gtttcttcca agctttgatt ggttggaaaa gtgtaacgtg tattaccctt gtccgaggca
    10321 tagtcaaaca ttgcttccgc atcatccaaa gttacaggtc taagcaataa acgctccgtt
    10381 tctatagacg gatactgggc aagttttaca aaaattgatt ccatacgttt cctccgcttg
    10441 aaacgtttct aatctaatat aaggatattg aataaaacat ttaaaaagca aatttatact
    10501 tagcattgag ataactttaa gtacaaaaga actatccttc agacagaggt tgagactcta
    10561 attcttcact ctcttctttt ttaactggag ctggttcata ggctcggata atctgagcga
    10621 caactggatg gcgaaccaca tccttggctg aaaaatgaac aaagtcaatc tgatggatgt
    10681 tcttgagttt ctcttgagca tcaatcaaac cggacttgac attacgtggc aggtcaatct
    10741 gactgatatc tccattgaca atcatcttag aatgaaagcc taaacgcgtc aagaacatct
    10801 tcatctgcat gatggtcgtg ttttgcgcct catcgagaat gacaaaggca tcatccaagg
    10861 tccggccacg catataggca aggggcgcaa tttcgataat ttcacgctcc atgagacgag
    10921 tcgtttggtc tttcccaaga atttgataca aggcatcgta aacaggacga aggtaaggat
    10981 ccaccttctc cttaagatca cccggaagaa aacctagact ctctcctgct tccaccgctg
    11041 ggcgtgtcag gataattcgt ttgacttgcc cacgtttaag ggcagtcact gccaaggtca
    11101 ctgcaaggaa ggtcttccct gtccctgcag gtccgattcc aaaggtcaca tcatgctgtt
    11161 tgacactgtc cacataaagt ttttgaccca aggttttgac tcggataggc ttcccagtat
    11221 tgtctttgat gatttcttct tcgtaaaggg cgacaaactt gtcaatttca tcatttttga
    11281 ccatgctaat cgcagtgact acatctggcg taccaacggt catcccacga tttaccaaga
    11341 ccatcaaagc ctgaataact tgacgggctt cctcacaggc agactcttct cccaaaacct
    11401 ggacaatctc cgtacgagca tgaatcacaa catcaagctc ttcttccatc aaacgaagat
    11461 ggcgttcatt ggaaccaaaa agatgaaaca ggtcatctgg atgactcagt tgaatgtcta
    11521 ttgaatgttc cttcaaataa agaacctctc taatcctttt atttcttttt attatagcag
    11581 agggatggag aaattactag cccaaaccca atgaataaat gctagtgttc tagatattcc
    11641 tgccaaatgc tgtcaaaatc tcctaggtta aaagagaaac tggcatgctc ctccaaaaag
    11701 cgactgacct catcaaaatc atctgtatgt tttggaaaag ctgcctcttc aaaagcgagg
    11761 tctgccaaaa ttgctttggg actgttactt ttaggattgc gctcggtcat gagccaagtg
    11821 taaaatgatt ttctcataag cctccctaaa tcaactctgt accaaactgg tcctgcttga
    11881 ttttaccagc cttcataaga ccacctaaag ctttcttgaa ctgaccttta gaaatgccaa
    11941 aggttgcctt gatgtcgtct ggagatgact tgtcatttaa ggtcatgaaa ccgccattgc
    12001 tttccaaata agtcaaaatc atctgagcat cgttttccaa catttcaaag gagcgtggtt
    12061 tgagggagag gttcagagtg cggtccactt cacggaaacc aataacgcgc gcatctaata
    12121 cttgccccaa acgtggctct gcgtaacgct cgctaggatg aataaaacca agcatattat
    12181 tttctggtag gtaaacaaaa gttcctgaca gcttgagacg gtaaacaatg gctggccagt
    12241 tttggttctg catgttgttg taagcaggac gagcaagacg ttggaagtct tcttgataag
    12301 ccaagaggcc ccagatacgg tctttcttat ccacttcaag acggatgtag agttggtcgc
    12361 ccttcttagg ccagagttcc ttgagctcag ggagaatatc gagtgacaca acgatttcct
    12421 tgtcaggaag gcctgtatcc acaaagacac ccaagtcctt acgaacctct gtgacacgtc
    12481 cccaaccaaa ttggtcctga gtggcagtca cttctaaggt tgtcaggcgg agtttttgct
    12541 tcatatccgt gtatgcaaaa cctttgaccg tatcccctac tgtatgttgg ccctcttcct
    12601 tagcaagagc ataggtttga ccatcctttt gcacaaagta aaaacggtca ttttcatcga
    12661 tgatcagtcc aacgataaaa cttgcaagat ttgtattcat atttccttct ttcgaataaa
    12721 actcagccag caatgccaac tgagtttttc tgtttatttt tagacttcca aaagttcttt
    12781 ctctttgtta gcagtcatgt cgtcgatgtg tttaacagca tcgtctgtta ctttttgaat
    12841 atctttttca agagtcttca attcgtcttc agtgatttct tttgcttttt cttgtttctt
    12901 agcttcgtcc atagcatcgc gacggatatt gcggatagcc actttagcat tttcgccgac
    12961 cttcttcact tctttagcaa ggtcacgacg agtttcttct gtaagagctg ggataaccaa
    13021 gcgaatcaca gaaccgtcat tagccggtgt gataccaaga tcagaagcgt tcaaggcacg
    13081 ttcgatgtct ttcaatgaag acttgtcaaa tggtgttacc aacaaaacac gcgcttctgg
    13141 aatcgtaatt gaagcgattt ggttaagagg agtttcgact ccatagtatt ctacatgtac
    13201 acggtcaagc aagcttgcat tggcacgacc agcacggata ccaccaaatt cacgagcaag
    13261 tgattggtga gactgggtca ttctctcttt agctttttca ataattacgt tagccatatt
    13321 ctttcttatt ccttttcttc gatattattt gaaactgttg ttccgatatt ttcaccaaat
    13381 acgacacgtt tgatgttgcc tgattggttc atgttgaaga caaccaagtc aatgtcgttg
    13441 tccattgaga gggttgaggc tgttgagtcc atgatacgaa gacctttgtt gataacatca
    13501 cggtgggtca attcttcaaa cttaacggct gtcttgtcct tcttaggatc ggcattgtac
    13561 acaccatcga cgccattttt agccatgagg atggcatctg cttcgatttc agctgcgcga
    13621 agggccgctg ttgtatctgt cgagaagtat ggtgaaccaa ttccagcacc aaagataacg
    13681 atacggcctt tttcaaggtg acgaagggca cgtccacgga cataaggctc tgccacttgt
    13741 tgcatagcaa tagctgtttg tacacgcgta tcaaccccaa cttgttgcaa tgaatctgcc
    13801 atcacaagag cattcataac agtcccaagc attccagtgt aatctgcctg aacacggtcc
    13861 atacctgctt ctgctgcagg ttctccacgc cagagatttc ctccaccaat aacaagggca
    13921 atttcgatac ctaagctatg aacttcttga atctcttttg cgattgtttg aactgtttgg
    13981 atatcaatcc ctacgccacg ttcaccggca agggcttcac ctgataactt gattaaaata
    14041 cgtttatact tgggattcgc cattttcact ctccttcttt catcctacct attttatcac
    14101 aatttctaag atttttatag tatcatgagc aattctttca aaaaaattag acagtcaaaa
    14161 attcctctaa gtcggcaagg gcacgctctg caattttttc ataacgagcc ttcttatcac
    14221 ggatacgctc gccttccaac tccttgatga tcccaaaatt gacattcatt ggttggaaat
    14281 gtttgctatc cgcatgggtg atataatgag ctaagcttcc aatcgctgtt gtttctggga
    14341 aaatagcctc gctttctccc ttaaatagtc gagccgcgtt aattcccgca accaagcctg
    14401 acgctgctga ctctacatag ccttccacac ctgtcatctg accagcaaag aagaggttgg
    14461 gctgtttctt ggaacgatag gtctgctcaa gaagattcgg tgaatccata taagaattgc
    14521 ggtgcataac tccataacga acaaactccg cattttcaag acctggaatc atttggaaga
    14581 cacgtttctg ttctccccat ttgaggtggg tctggaaacc aacaatattg tagaggctac
    14641 cagctgcatt gtcctgacga agttgcacaa ccgcataagg tgttttaaat tctccatcac
    14701 gaggtcctgt atagtcgtct gggtactcaa gaccgactgg cttcataggg ccataaagca
    14761 tagttttaat gccacgtttg gccatgactt cgataggcat acatccttca aagtactttt
    14821 ctttttcaaa agaattaagc ggtgcttctt ctgcattgac caaagcttca tggaaatcca
    14881 taaattcttg cttggtcata ggggcattga ggtaggccgc ttctccctta tcataacgtg
    14941 atttgaggta gaccttgctc atatcgatag tgttgacatc gataataggc gctgccgcat
    15001 cgtagaaata aaaaccatca ccgtcattaa gggcatgaat cttttcagcc aaggcatcac
    15061 ttgtcaaagg accagtagcg ataaccgtaa taacatctgt cggcaattct gtaatttcat
    15121 cacgaaccac ttcaatcaag gggtggttgg caactttttc ggtcaccatt tgagagaaac
    15181 catcacggtc cactgcaagg gcaccacctg caggaacacg tgtagcctca gcagattcca
    15241 aaatcacaga acccaagcga cgcatttctt ccttaaggag gccaacagca tttgtcaaag
    15301 catctccacg caaggaattg gaacaaacca actcggcaaa gttgtctgtt ttgtgctgag
    15361 gtgttgactt gacaccacgc atttcataaa gtttaactgg aataccacgc tctgcgattt
    15421 ggtaggctgc ttcagaacct gccaaaccag caccgataac attgatataa gattgagaca
    15481 cgacactaat acctctttgg gtgggaccta agtccatatt aaaacacccc actgggctgt
    15541 ttgacaccca caaatctttc taattttttc ttctactagt ataacaaaaa aagggaagaa
    15601 ggcaaacttc cctgtttagt cattttcttg atgtaaagag atagtgagta ttccagttaa
    15661 gaatcaatta tatgctactc tataaaatct tttccacata acggatcgat agggactgtt
    15721 attctatctc ttgctataac cgtattatct aaaacagcat aacattcaac atagtggtct
    15781 cctttaaact gagaatcttc gtgatgtttt attttacctg aaaaaatagc accacgctca
    15841 caatttcttc taatcgcctc agcttcaata tttcttactt tccaatagac tccctgcgaa
    15901 acaaaattat gaagcaagta aacaactaac cgatgcacga tttaagcgtc ttgttggtgt
    15961 tcagcgcacg acttttgaag agatgttagc tgtattaaaa acagcttatc aacttaaaca
    16021 cgcaaaaggt ggacgaaaac ctaaattaag cctagaagac cttcttatgg ctactcttca
    16081 atatgtgcga gaatatcgaa cttatgaaga aattgcggct gattttggta ttcacgaaag
    16141 caacttactc cgtcggagcc aatgggttga agtaactctt gttcaaagtg gtgttacgat
    16201 ttcaagaact cctctcagct ctgaggacac ggtaatgatt gatgcgacgg aagtaaaaat
    16261 caatcgccct aaaaaaagaa ttagcgaatg attctggtaa aaagaaattt cacgctatga
    16321 agactcaagc gattgtcaca agtcaaggga gaattgtttc tttggatatc actgtgaact
    16381 attgtcatga tatgaagttg ttcaaaatga gtcgcagaaa tatcggacaa gctggtaaaa
    16441 tcttggctga cagtggttat caagggctca tgaagatata tcctcaagca caaacttcac
    16501 gtaaatccag caaactcaaa ccgctaacag ttgaagataa agcctgtaac catgcgctat
    16561 ctaaggagag aagcaaggtt gagaacatct ttgccaaagt aaaaacgttt aaaatgtttt
    16621 caacaac
//
LOCUS       DAVLVP010000037        16574 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.037, whole genome shotgun sequence.
ACCESSION   DAVLVP010000037 DAVLVP010000000
VERSION     DAVLVP010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16574
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.037"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            159..830
                     /gene="sdaAB"
                     /locus_tag="VYN69_001154"
     CDS             159..830
                     /gene="sdaAB"
                     /locus_tag="VYN69_001154"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015606087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEV1533433.1"
                     /translation="MKSLRFQSVFDIIGPVMIGPSSSHTAGAVRIGKIVSSIFDDTPT
                     EVEFQLFNSFAKTYRGHGTDLALVAGILGMDTDDPEIPNSLEIAHKRGIKIVWTIQKD
                     SNAPHPNTTKITVKNAHKTISVTGISIGGGNIQVTELNGFAVSLNMNTPTIIIVHQDI
                     PGMIALVTEALSRYGINIAQMNVTREKAGEKAIMIIEVDSRNCDEAIEEIRKIPHLHN
                     VNFFK"
     gene            839..1711
                     /gene="sdaAA"
                     /locus_tag="VYN69_001155"
     CDS             839..1711
                     /gene="sdaAA"
                     /locus_tag="VYN69_001155"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEV1533434.1"
                     /translation="MFYSIKELVEQADLDFQGNVAELMITTEFELTGREREEVFLLME
                     RNLEVMKASVQLGLNENKSRSGLTGGDAAKLDHYIKNEKTLSDYTILSAARNAIAVNE
                     HNAKMGLVCATPTAGSAGCLPSVLTAAIEKLDLSHEQQLDFLFAAGAFGLVIANNASI
                     SGAEGGCQAEVGSASAMSAAALTLAAGGTPYQASQAIAFVIKNMLGLICDPVAGLVEV
                     PCVKRNAMGASFAFIAADMALAGIESKIPVDEVIDAMYQVGASMPTAFRETAEGGLAT
                     TPTGRRLQKEIFGE"
     gene            1733..2365
                     /locus_tag="VYN69_001156"
     CDS             1733..2365
                     /locus_tag="VYN69_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEV1533435.1"
                     /translation="MTSITAIFFDLDGTLVDSSIGIHNAFTYTFKELGVPSPDAKTIR
                     GFMGPPLESSFATCLSKDQISEAMQIYRSYYKAKGIYEAQLFPQIIDLLEELSSSYPL
                     YITTTKNTSTAQDMAKNLEIHHFFDGIYGSSPEAPHKADVIHQALQTHQLAPEQAIII
                     GDTKFDMLGARETGIQKLAVTWGFGEQADLLNYQPDYIAHKPLEVLAYFQ"
     gene            complement(2623..4473)
                     /locus_tag="VYN69_001157"
     CDS             complement(2623..4473)
                     /locus_tag="VYN69_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEV1533436.1"
                     /translation="MNKKLTDYVIDLVEILNKQQKQVFWGIFDIFSMVVSIIVSYILF
                     YGLINPAPVDYIIYTSLAFLFYQLMIGFWGLNASISRYSKITDFMKIFFGVTASSVLS
                     YSICYAFLPLFSIRFIILFILLSTFLILLPRITWQLIYSRRKKGSGDGEHRRTFLIGA
                     GDGGALFMDSYQHPTSELELVGILDKDSKKKGQKLGGIPVLGSYDNLPELAKRHQIER
                     VIVAIPSLDPSEYERILQMCNKLGVKCYKMPKVETVVQGLHQAGTGFQKIDITDLLGR
                     QEIRLDESRLGAELTGKTILVTGAGGSIGSEICRQVSRFNPERIVLLGHGENSIYLVY
                     HELIRKFQGIDYVPVIADIQDYDRLLQVFEQYKPAIVYHAAAHKHVPMMERNPKEAFK
                     NNIRGTYNVAKAVDEAKVSKMVMISTDKAVNPPNVMGATKRVAELIVTGFNQRSQSTY
                     CAVRFGNVLGSRGSVIPVFERQIAEGGPVTVTDFRMTRYFMTIPEASRLVIHAGAYAK
                     DGEVFILDMGKPVKIYDLAKKMVLLSGHTESEIPIVEVGIRPGEKLYEELLVSTELVD
                     NQVMDKIFVGKVNVMPLESINQKIGEFRTLSGDELKQAIIAFANQTTHIE"
     gene            complement(4512..5672)
                     /locus_tag="VYN69_001158"
     CDS             complement(4512..5672)
                     /locus_tag="VYN69_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1533437.1"
                     /translation="MSSKVSGFQDKNGKVLKIMIEWWKEKFRRVVVTQNVESLLVSIV
                     ISAYNEEKYLPGLIEDLKNQTYPKENIEILFINAMSTDGTTAIIQQFIKEDTEFNSIR
                     LYNNPKKNQASGFNLGVKHSVGDLILKIDAHSKVTETFVMNNVAIIQQGEFVCGGPRP
                     TIVEGKGKWAETLHLVEENMFGSSIANYRNSSEDRYVSSIFHGMYKREVFQKVGLVNE
                     QLGRTEDNDIHYRIREYGYKIRYSPSILSYQYIRPTFKKMLHQKYLNGLWIGLTSHVQ
                     FKCLSLFHYVPCLFVLSLVFSLALLPITFVFITLLLGAYFLLLSLLTLLTLLKHKNGF
                     LIVMPFLLFSIHFAYGFGTIVGLIRGFKWKKEYKGTIIYLDKISQINQNMLQ"
     gene            5730..6896
                     /locus_tag="VYN69_001159"
     CDS             5730..6896
                     /locus_tag="VYN69_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CynX/NimT family MFS transporter"
                     /protein_id="HEV1533438.1"
                     /translation="MKKQSLFFVPGIILIGVSLRTPFTVLPIILGNISQGLEVEVSSL
                     GVLTSLPLLMFTLFSPFSTQLAQKIGLEHLFTYSLFFLTIGSLIRLINLPLLYLGTLM
                     VGASVAVINVLLPSLIQANQPKKIGFLTTLYVTSMGIATALASYLAVPITQASSWKGL
                     ILLLTLLCLATFLVWLPNHRYNHRLAPQTKQKSQIKVMRNKQVWAIIIFSGFQSLIFY
                     TVMTWLPTMSIHAGLSSHEAGLLTSILSLISIPFSMTIPSLTTSLSTRNRQLMLTLVS
                     LAGVVGISMLFFPINNFIYWLAIHLLIGTATSALFPYLMVNFSLKTSAPEKTAQLSGL
                     SQTGGYILAAFGPTLFGYSFDLFHSWVPSVAALLLIDILMTVALFTVDRADKIL"
     gene            7035..7361
                     /locus_tag="VYN69_001160"
     CDS             7035..7361
                     /locus_tag="VYN69_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEV1533439.1"
                     /translation="MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIANIKESTLY
                     PILKKLEGNSFLTTYSREFQGRMRKYYSLTNGGIEQLLTLKDEWALYTDTINGIIEGS
                     IRHDKN"
     gene            7348..7941
                     /locus_tag="VYN69_001161"
     CDS             7348..7941
                     /locus_tag="VYN69_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEV1533440.1"
                     /translation="MTRTEYLTQLELYLKKLPEADRIEAMDYFRELFDDAGVEGEEEL
                     IASLGTPKEAAHEVLSNLLDKKINEAPAQKNNRQILHIALLALLAAPIGIPLGIAILV
                     TLFAILVAALTVILAFFAVSILGIIGGFLFLVESFTVLAQAKSAFILIFGSGLLAIGA
                     SSLVLLGISYVTRFFGLLIVRLVQFVLKKGKRGNQHA"
     gene            7934..8845
                     /locus_tag="VYN69_001162"
     CDS             7934..8845
                     /locus_tag="VYN69_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001233690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 domain-containing protein"
                     /protein_id="HEV1533441.1"
                     /translation="MRKWTKGFLIFGVATTVIGFILLFVGIQSDGIKSLLSMSKEPVY
                     DSRTEKLTFGKEVENLEITLHQHALTITDSFDDQIHISYHPSLSANHDLITNQNDRTL
                     SLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILQLPKGRTLKGINISANRGQTTI
                     INASLENATLNTNSYILRIEGSRIKNSKLTTPNIVNIFDTVLTDSQLESTENHFHAEN
                     IQVHGKVELTAKDYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYK
                     TEKTDVKDQLIARSDDNIDLISTPSRR"
     gene            8908..9972
                     /locus_tag="VYN69_001163"
     CDS             8908..9972
                     /locus_tag="VYN69_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533442.1"
                     /translation="MKQEWFESNDFVKTTSKNKPEERAQEVADKAEETIANLDTPIGK
                     NTQVEEEVSQAEVELESQQEEKIEAPEDSEARTGIEEKKASNSTEEEPDLSKETEKVT
                     IAEESQEALPQQKATTKEPLLISKSLESPYIPDQAPKSRDKWKEQVLDFWSWLVEAIK
                     SPTSKLETSITHSYTAFLLLILFSASSFFFSIYHIKHAYYGHIASINSRFPEQLAPLT
                     LFSIVSILVATTLFFFSFLLGSFVVRRFIHQEKDWTLDKVLQQYSQLLAIPIFLTAIA
                     SFFAFFDSLRFTALLCVISIGIILLASLHIITRPSQASETDSFYQLFLSVLVNGVIIL
                     LFFVAEVALIGDYLRILAFL"
     gene            complement(10053..10904)
                     /locus_tag="VYN69_001164"
     CDS             complement(10053..10904)
                     /locus_tag="VYN69_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4299 domain-containing protein"
                     /protein_id="HEV1533443.1"
                     /translation="MAKTFFIPNKQSILGEQEILNAKSILALLDGLESHSYDVVYLRQ
                     PLNRLEYIECAIVGQSQFLFKVSYADGQKAYRVDVPDLLTKTDWQIIKSFLDALLAYT
                     GTDIEGLDGFDFEAYFQASIQAYLADPVARFTICQGIFNPIFFSRENLKSFLEADGLA
                     QFEARVRAVQETDAYFARVSFYQDGEGKVHGVYHLAQGVKTVLPREPFVPAAYIEQLV
                     DKEVQWEIDLVQITGDGSKPEDYESIARLDYAKFLEVLPPSFYHQLDANQIEIQPILV
                     QDFKTLA"
     gene            complement(11026..12012)
                     /locus_tag="VYN69_001165"
     CDS             complement(11026..12012)
                     /locus_tag="VYN69_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEV1533444.1"
                     /translation="MAKDIRVLLYYLYTPIENAEQFAADHLAFCKSIGLKGRILVADE
                     GINGTVSGDYETTQKYMDYVHSLPGMEELWFKIDEESEQAFKKMFVRYKKEIVHLGLE
                     DNDFDNDINPLETTGAYLSPKEFKEALLDKDTVVLDTRNDYEYDLGHFRGAIRPDIRN
                     FRELPQWVRDNKEKFMDKRVVVYCTGGVRCEKFSGWMVREGYKDVGQLHGGIATYGKD
                     PEVQGELWDGKMYVFDERIAVDVNHVNPTIVGKDWFDGTPCERYVNCGNPFCNRRILT
                     SEENEDKYLRGCSHECRVHPRNRYVSKNELTQAEVIERLAAIGESLDQAATV"
     gene            complement(12284..13759)
                     /locus_tag="VYN69_001166"
     CDS             complement(12284..13759)
                     /locus_tag="VYN69_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3502 domain-containing protein"
                     /protein_id="HEV1533445.1"
                     /translation="MKNWKKYAFASASVVALAAGLAACGNLTGNSKKAADSGDKSVIK
                     MYQIGDKPDNLDELLANANKIIEEKVGAKLDIQYLGWGDYGKKMSVITSSGENYDIAF
                     ADNYIVNAQKGAYADLTELYKKEGKDLYKALDPAYIKGNTVNGKIYAVPVAANVASSQ
                     NFAFNGTLLAKYGIDISGVTSYETLEPVLKQIKEKAPDVVPFAIGKVFIPSDNFDYPV
                     ANGLPFVIDLEGDTTKVVNRYEVPRFKEHLKTLHKFYEAGYIPKDVATSDTSFDLQQD
                     TWFVREETVGPADYGNSLLSRVANKDIQIKPITNFIKKNQTTQVANFVISNNSKNKEK
                     SMEILNLLNTNPELLNGLVYGPEGKNWEKIEGKENRVRVLDGYKGNTHMGGWNTGNNW
                     ILYINENVTDQQIENSKKELAEAKESPALGFIFNTDNVKSEISAIANTMQQFDTAINT
                     GTVDPDKAIPELMEKLKSEGAYEKVLNEMQKQYDEFLKNKK"
     gene            complement(13863..14786)
                     /locus_tag="VYN69_001167"
     CDS             complement(13863..14786)
                     /locus_tag="VYN69_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001075101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEV1533446.1"
                     /translation="MAKKKIKKEKIDNVGIHSFSKKADIFFSIISGLIALSCILPFVF
                     VIIISVTDEKSLLQYGYSFFPSQFGLDGFEFLAQFKDKILQALFISVFVTVVGTLTNV
                     FITTTYAYAISRTTFKYRRFFTIFVLLSMLFNAGLVPGYIMVTRVLQLGDTVWALIVP
                     MLLSPFNIILMRSFFKKTIPEAILESARIDGASEARIFFQICLPLSLPGIATITLLTA
                     LGFWNDWFNALLYIKSDNLYPLQYLLMQIQQNMDYIAKAVGLTGQLGVALPKETGRMA
                     MVVVATLPIAILYPFFQRYFVKGLTIGGVKE"
     gene            complement(14800..15729)
                     /locus_tag="VYN69_001168"
     CDS             complement(14800..15729)
                     /locus_tag="VYN69_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEV1533447.1"
                     /translation="MKKFSKTLRDNWIFLLMVLPGALWLILFFYIPVFGNVVAFKDYH
                     MTSNGFIDSIINSKWVGLDNFRFLFSSRDAFIITRNTVLYNLGFIFLGLVVSVGIAII
                     LSELRSKRMVKIFQTSMLFPYFLSWVIISFFTDAFLNIDKGVFNHLLESLGLKEVNFY
                     ADLGIWPYLLLFLGIWKGFGYSSVMYYATIMGIDPTYYEAATVDGASKWQRIRNVTIP
                     QLTPLVTVLTILAVGNIFRADFGLFYQIPHNAGQLYNVTNVLDVYVFNGLTQTADIGM
                     AAAAGLYQSVVGLILVILSNLLARRVDPNSALF"
     gene            complement(16048..16269)
                     /locus_tag="VYN69_001169"
     CDS             complement(16048..16269)
                     /locus_tag="VYN69_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533448.1"
                     /translation="MSLQIKLKKLAKELSKLLKDSNLETVDKDVLENSQEELQKAVLF
                     LADEKGSEHTAAELIDNLKEVIAKLKANA"
     gene            complement(16223..16483)
                     /locus_tag="VYN69_001170"
     CDS             complement(16223..16483)
                     /locus_tag="VYN69_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533449.1"
                     /translation="MTGVSNPTTDSARLVLADSKKAFADDSLIEQGLRDILQTVKDAI
                     ASLESIKESQSATKDGGQTAGKETAGEDESANQTQEISQGIK"
BASE COUNT         4931 a         3576 c         3106 g         4961 t
ORIGIN      
        1 caaaattatt ttttatttcc ctaaataagg tgttttttgt cataaaagat actattttta
       61 tgctatttga ggtattagaa aggttttcat ccacaattaa agccaagccc ctcattcttt
      121 gatataatag agataagaat ttttataagg ggaaactgat gaaatcactt cgttttcaat
      181 ctgtctttga tatcatcgga ccagttatga ttggcccatc tagtagccat accgctggtg
      241 ctgttcgtat tgggaagatt gtctcttcca tttttgatga tactccgaca gaagttgaat
      301 tccaactttt taactcattt gccaagacct atcgtggtca cggaacagac ctagcccttg
      361 ttgcaggtat tttgggaatg gatacagatg atcctgaaat tccaaatagt ctggaaattg
      421 cccacaagcg tggtatcaag attgtctgga ccattcagaa agacagcaat gcccctcacc
      481 ctaacaccac taaaattact gttaaaaatg cccacaagac catcagcgtg actggtattt
      541 ctatcggtgg aggaaatatt caggtaaccg aactcaatgg ttttgccgtc tctctcaata
      601 tgaatacacc gactatcatc atcgttcatc aagatattcc aggtatgatt gcccttgtta
      661 cagaggcgct ttcccgctat ggtatcaata tcgcccagat gaatgtcact cgtgaaaaag
      721 ctggtgaaaa agccattatg attatcgaag ttgacagtcg caactgtgat gaggctatcg
      781 aagaaattcg aaaaatccct catctccaca atgtcaattt ctttaaatag gaggaagcat
      841 gttttattct atcaaagaat tggtcgagca agcagatctg gactttcaag gaaatgtcgc
      901 agaactcatg attacaacag agtttgaatt gaccggtcgc gaacgtgaag aagtcttcct
      961 tctcatggaa cgcaatctgg aagttatgaa agcctctgtc caacttggcc tcaatgaaaa
     1021 taaatctcgt agtggcctga caggtggaga tgctgccaaa ttggatcact acatcaaaaa
     1081 cgaaaaaact ttatcagatt acacgattct ctctgctgcc cgaaatgcta tcgcagtcaa
     1141 tgaacacaat gctaaaatgg gcttggtctg cgctactcct accgcaggaa gtgccggttg
     1201 tctcccttcc gttctcactg ctgctattga aaaattagac ctcagccacg agcaacaatt
     1261 ggatttcctc tttgctgctg gtgcctttgg actagtcatc gcaaacaatg cctccatctc
     1321 aggtgctgag ggtgggtgtc aagctgaagt tggttcagcc tctgctatga gtgctgccgc
     1381 cttgactctg gctgcaggtg gaacacctta tcaggccagt caagctattg cctttgtcat
     1441 taaaaatatg ctaggcctca tctgtgaccc tgttgcaggt ttggtcgaag ttccctgtgt
     1501 caaacgtaat gccatgggag ctagctttgc tttcatcgca gcagacatgg ccttggcagg
     1561 tatcgaatct aaaatccctg tggatgaagt gatcgatgcc atgtaccaag taggagcaag
     1621 catgccaact gcctttcgtg aaacagctga aggtggactc gctaccaccc ctactggtcg
     1681 tcgcctccaa aaagagattt tcggagaata agcttatcaa ataggagaaa ccatgacctc
     1741 tatcacagcg atttttttcg atttggatgg aaccctcgtt gatagttcta tcgggattca
     1801 caatgccttt acctatacct ttaaagagct gggggtgcct agccctgatg ccaaaactat
     1861 tcgtggtttt atgggaccac ctctcgaaag tagttttgcg acctgcctgt ccaaagacca
     1921 aatttctgaa gccatgcaga tatatcgttc ttactataag gcaaaaggca tctatgaagc
     1981 tcaactcttt cctcagatta tagacttgct tgaggagtta tcgagcagtt atccactcta
     2041 catcaccacg acaaagaata catctactgc tcaagacatg gccaaaaact tggaaatcca
     2101 tcatttcttt gatggtattt atggttctag ccctgaagca ccccataagg cagatgtcat
     2161 tcaccaagcc ttacagacac atcaactagc accagaacaa gccatcatca tcggagatac
     2221 caagtttgat atgctgggag ctcgagaaac aggtattcag aaattggccg tcacttgggg
     2281 atttggagag caagcagatc tactaaacta tcaacctgat tatatcgctc acaaaccctt
     2341 agaagttttg gcatattttc aatagcatag actgggtaac aattccattt cagaagaaaa
     2401 tgaagtaata ctcaatgaaa atcaaagagc aaactaggaa actagctgcg ggctgctcta
     2461 aacactgttt taaggttgtg gatagaactg acgaagtcag taaccatata cggcaaggcg
     2521 acgttgacgt ggtttgaaga gattttcgaa gagtataagt ctctacataa taaaacgcat
     2581 attaccaagg ttgtgtaact tgatactatg cgtttttctt ttttattcaa tgtgggttgt
     2641 ttgattagca aaggcgataa tagcttgctt caactcatct ccactgagag tgcggaactc
     2701 tccaatcttt tgattgatgg attctaaagg catgacatta accttaccaa cgaaaatctt
     2761 atccataact tgattatcaa caagttcggt tgataccaag agttcttcgt agagtttttc
     2821 acctgggcgg attccaactt caacgattgg aatttcactt tcagtgtggc cacttagaag
     2881 caccatcttc ttggccaagt cataaatctt gactggtttg cccatatcaa ggataaagac
     2941 ttccccatct ttggcataag caccagcatg gataaccaga cggctagctt ctggaatggt
     3001 cataaagtaa cgggtcatgc ggaagtctgt caccgttaca ggcccacctt cagcaatctg
     3061 acgttcaaag actggaatga cactaccacg gctaccaaga acattcccaa aacgaactgc
     3121 acagtaggtt gattggctac gttggttaaa gccagtgaca atcaactccg ccacgcgctt
     3181 ggttgctccc ataacatttg gtggattaac tgccttatct gtcgaaatca taaccatctt
     3241 agacacttta gcttcatcaa cagccttagc aacattgtaa gttccacgga tattgttttt
     3301 gaaggcttct tttggattgc gctccatcat aggaacatgc ttgtgggctg ccgcatgata
     3361 aacaatagca ggtttgtact gctcaaagac ttgcaacaaa cgatcatagt cttgaatgtc
     3421 cgcaatcaca ggtacataat caatcccttg gaacttacga atcaattcat gataaacaag
     3481 gtagattgag ttttccccat gaccgagcaa gacaatgcgt tcaggattga agcgactaac
     3541 ttgacgacag atttcagaac cgattgaacc tccagctcct gtgactaaga tggtcttacc
     3601 tgtcagttct gcacccagac gcgattcgtc aagacggatt tcctgacgac ccaaaaggtc
     3661 cgtaatatca attttttgga agccagtacc tgcttggtga aggccctgaa caacagtttc
     3721 aaccttaggc atcttgtaac atttgacacc cagcttatta cacatctgca agatacgctc
     3781 atattctgac ggatccagcg acggaatcgc aacgatgaca cgctcgattt gatggcgttt
     3841 ggctaattca ggcagattgt cataagagcc caaaacagga ataccaccaa gtttttgacc
     3901 ctttttctta gaatccttat ccaaaatacc gaccagttct aattcactgg ttggatgttg
     3961 gtaactatcc ataaagagag ccccaccatc accggcacca atcaagaagg tccgacggtg
     4021 ttctccatca ccactacctt ttttgcgtct ggagtagatt aactgccaag taatccgtgg
     4081 caataaaatc aagaaggtac tcaacaagat aaagagaatg atgaaacgga tggagaagag
     4141 tggcaagaag gcataacaga tactatatga caagacactg ctagcagtca caccaaaaaa
     4201 gattttcatg aaatccgtaa tcttgctgta acgactaatg ctcgcgttca atccccaaaa
     4261 accaatcatc aattgataga acaggaaggc caaactcgta tagataatgt agtcaacagg
     4321 tgctggatta atcagcccat aaaataaaat ataagataca attatggaaa ccaccatact
     4381 gaaaatatca aatattcccc agaaaacctg cttttgttgt ttatttaaaa tttccaccag
     4441 atcaatcaca taatctgtta gttttttatt catagaaatc tactcctcct taaaagagtt
     4501 ttactatatt gttattgtag catattttga tttatttggc ttattttatc caaataaatt
     4561 attgttccct tgtactcctt cttccattta aatcctctaa ttaaacctac aatcgtccca
     4621 aagccataag caaagtgaat ggaaaataaa agaaagggca tcacaattag aaatccattt
     4681 ttatgtttta ataaagtcag caaagtgagt agtgacaaaa gtagaaaata ggcacctaat
     4741 agtaaagtta tgaatacgaa tgtgatcggt aacaatgcta gactaaacac aagactcaaa
     4801 acaaataaac aaggaacata gtgaaataat gataaacact taaactgaac atgacttgtc
     4861 aagccaatcc acaaaccatt taaatacttt tgatgcagca ttttcttgaa tgttggtcga
     4921 atatactgat aagatagaat acttgggcta tagcggattt tataaccata ttctcgaatt
     4981 ctataatgaa tatcattatc ttcagttcgg ccaagttgct catttactaa accaaccttc
     5041 tggaaaacct ctcgtttata cattccatga aaaatagaag aaacatatct atcctcagaa
     5101 ctatttcgat aattggcaat gctactgcca aacatatttt cctcaacaag atgcaaggtc
     5161 tctgcccatt ttccttttcc ttcgacaatc gtcggtctag gccccccaca gacaaattca
     5221 ccttgttgaa taatagccac attgttcatt acaaaagtct cagtaacttt tgaatgagca
     5281 tcaattttta aaataaggtc ccctacagaa tgtttaactc ccaggttaaa accactagct
     5341 tgatttttct taggattgtt atacaatcta attgagttaa actctgtatc ttcctttata
     5401 aattgctgaa tgatagctgt ggtcccatct gtggacatag catttataaa tagaatttca
     5461 atattctctt taggataggt ttgatttttt aagtcttcaa ttagaccagg cagatatttt
     5521 tcttcattgt atgcactgat tacaatggat acgagaagac tttcaacatt ttgagtcact
     5581 actactctcc tgaatttttc cttccaccat tctatcataa ttttcaagac tttcccattt
     5641 ttatcttgaa agccagaaac cttacttgac attatagtga aaaagcctta aaataagctg
     5701 ttattaaaat cagctagaag aggtattata tgaaaaagca atcactcttt tttgttccag
     5761 gtattatcct gattggtgtt tccttgcgaa ctccttttac tgttttaccc attattttgg
     5821 gaaatatttc gcaaggtctg gaggtagaag ttagttcgct tggtgtcttg accagcctgc
     5881 ctctccttat gtttaccctc ttttcaccat tttctaccca actggctcag aaaatcggct
     5941 tggagcatct cttcacctac agcctcttct tcttgaccat cggctcactt attcgactaa
     6001 tcaatctgcc cctgctctat ctaggaacct tgatggttgg ggcaagtgtc gcagtcatca
     6061 atgtcctgct tcctagtctt atccaagcca atcaaccaaa gaaaattggt tttctgacca
     6121 ccttatatgt aacgtctatg gggattgcaa cggctctggc ttcctatcta gctgtgccca
     6181 ttacacaagc cagttcttgg aaaggactta tccttctcct cacgttactc tgtctagcaa
     6241 cttttttggt ctggctccca aatcaccgct ataatcatag actagctcca caaaccaaac
     6301 aaaaaagtca aataaaggtc atgcgtaata aacaggtttg ggcaattatt atcttttcag
     6361 gttttcaatc cttgatcttt tacaccgtca tgacctggtt acctaccatg tctatccatg
     6421 caggtctatc cagtcacgaa gctggcttgc tgacttctat cttatctctg attagcattc
     6481 ctttttcaat gaccatccca agcctgacaa ccagtttatc tactcgcaac cgtcagctca
     6541 tgctcactct ggtttcacta gctggtgtgg tcggcatttc catgctcttt ttcccaatca
     6601 ataatttcat ttactggctt gccatccatc tcctcatcgg aaccgcaacc agtgccctct
     6661 tcccttatct catggtcaac ttttcactca agacaagcgc ccctgaaaag acagcccaat
     6721 tgtccggcct atctcaaaca ggaggctata tcctagcagc ctttgggcca accctctttg
     6781 gttacagttt tgacctgttc cactcttggg taccatctgt agctgccctc ttgctcatcg
     6841 atatcctgat gactgtggcc ctctttacag tggacagagc ggataagatc ctttaaactt
     6901 ctagttttag ctagaagttt tttatatata aaaattttac acatttctct tgacagggct
     6961 ttcttttaga tgtacaatgt atgtgtagaa aaattatata taaaaatctt acacattaga
     7021 aaaggaggtt tcccatgtac tttccaacat cctctgcctt gattgaattt ctcatcttgg
     7081 ctgtactgga gcagggtgat tcttatggtt atgagattag ccaaaccatt aagctgatcg
     7141 ctaatatcaa agaatccaca ctctatccca ttctcaaaaa attggaaggc aatagctttc
     7201 tgacaaccta ttctagagag ttccaaggtc gcatgcgcaa atactactcc ttgacaaacg
     7261 gtggtataga gcagctcttg accctaaaag atgaatgggc actctataca gacaccatca
     7321 atggcatcat agaagggagt atccgccatg acaagaactg aatacctgac tcagctagaa
     7381 ctctatctca agaaactacc tgaagctgac cgtatcgaag ccatggacta tttcagagag
     7441 ctctttgacg atgctggagt cgaaggagaa gaagaactca tcgctagttt gggaactccc
     7501 aaagaagcgg cccacgaagt tctatccaat cttctcgata aaaaaatcaa tgaagcaccc
     7561 gctcaaaaaa ataaccgaca aattttacat atcgccttgt tagccctcct tgcagcacct
     7621 atcggcattc ctctgggaat tgccatcctc gtgaccctgt tcgcaattct tgtagccgct
     7681 ttgactgtca ttctggcttt ctttgcagtt tccatactgg gtatcatcgg cggattccta
     7741 tttttagttg aaagtttcac tgtcctcgcc caagccaaat cagcctttat cttgattttt
     7801 ggttctggtt tactggctat cggtgcttct tcgctagttt tacttggcat ttcctatgta
     7861 actcgcttct tcggtctact cattgttcgt ctggtacaat ttgttcttaa aaaaggaaag
     7921 agaggtaatc agcatgcgta aatggacaaa aggatttctc atctttggtg tggcgactac
     7981 cgttatcggc tttatcctgc tttttgtagg tatccaatct gacgggatta agagcctact
     8041 ttccatgtcc aaagaacctg tctatgatag ccgtacggaa aagctaacct ttggcaagga
     8101 agtcgaaaac ctagaaatta ctctccacca acacgcgctc accatcacag actctttcga
     8161 tgatcaaatc cacatttctt accatccatc tctttctgct aaccatgatc ttatcacaaa
     8221 tcaaaacgat agaactctga gtctcactga taagaaactg tctgaaactc cgtttctctc
     8281 ttctggaatt ggtgggattc ttcatatcgc aagtagctac tctagtcgtt ttgaagaagt
     8341 tattctccag ctaccaaaag ggagaactct aaaagggatc aacatctcag ccaatcgcgg
     8401 acaaaccacc atcataaatg ctagccttga aaatgcgacc ctcaatacaa acagctatat
     8461 cctccgaatt gaaggaagtc gtatcaaaaa cagtaaactc acaacgccca atatcgttaa
     8521 tatctttgat acagttctta cagatagtca gctagagtca acagagaatc acttccacgc
     8581 tgaaaatatc caagtccatg gcaaggttga actgactgcc aaagattatc tcagaatcat
     8641 cctagaccag aaagaaagcc aacgaattaa ctgggacatc tcaagcaact atggttctat
     8701 cttccaattc acaagagaaa agcctgaatc aagaggtacg gaattaagca acccttacaa
     8761 aactgaaaaa accgatgtca aggatcaact cattgcgaga tctgatgata atattgatct
     8821 aatatccaca ccaagcagac gttgacaaaa acgcttacat ttgctactat ggaaacatat
     8881 ttatctaact aaaaaggagg ttctaccatg aaacaagaat ggtttgaaag taatgatttt
     8941 gtaaaaacaa caagcaagaa caagcctgaa gagcgagctc aagaggttgc agacaaggct
     9001 gaagaaacga tagccaatct cgatacacca attggaaaaa atactcaagt agaggaggaa
     9061 gtctctcaag ctgaagtcga actggaaagc caacaagaag agaaaattga agctcctgaa
     9121 gacagtgaag cgagaacagg aatagaagaa aagaaggcat ctaattctac tgaagaagag
     9181 ccagaccttt ctaaagaaac agaaaaagtc actatagctg aagagagcca agaagctctt
     9241 cctcagcaaa aagcaaccac gaaagagcca cttcttatca gtaaatcttt agaaagtcct
     9301 tatatccccg accaagctcc aaaatctagg gataaatgga aagagcaagt gcttgatttt
     9361 tggtcttggc tagtggaagc gatcaaatct cctacaagta agttggaaac aagtatcaca
     9421 cacagttaca cagcctttct cttgctcatt ctgttttctg catcttcctt tttctttagt
     9481 atctatcaca tcaaacatgc ttactatgga catatagcaa gcattaacag tcgcttccct
     9541 gagcagctag ctcctttaac tcttttttct atcgtctcta tcctagtagc gacaacactc
     9601 ttcttctttt cattcctctt gggtagtttc gttgtgagac gatttatcca ccaggaaaag
     9661 gactggacgc tagacaaggt tctccaacaa tatagtcaac tcttggcaat tccaatcttc
     9721 ctcactgcta ttgctagttt ctttgctttc tttgatagcc tacgatttac agccctcttg
     9781 tgtgtgatta gcattggaat cattctgctt gccagtctcc atatcattac aagaccaagt
     9841 caagcaagtg aaaccgactc cttctatcaa ttattcttgt ctgtccttgt gaacggagtt
     9901 attatcctcc tcttctttgt agctgaagtg gcactgattg gagattatct tcgtatcttg
     9961 gcctttcttt aaaattcaat cctgtcattc ttggcaggat tttttctatg tcaaaaaagc
    10021 aagtcgattg acctgcttct gctttacttt tcttatgcta atgttttaaa atcttgtact
    10081 aggatgggtt gtatttctat ttgattggcg tctagctggt ggtaaaaaga tgggggtaac
    10141 acctctaaga attttgcata gtccaagcga gctatggatt catagtcttc tggtttagag
    10201 ccgtctcctg tgatttgaac caagtcaatc tcccactgga cttccttatc caccaattgc
    10261 tcaatatagg ctgcaggaac aaacggttct ctcggtaaaa ctgtcttgac tccttgagct
    10321 agatggtaaa cgccatgcac ttttccttct ccatcctgat agaaggaaac tctcgcaaag
    10381 taggcatctg tctcttgaac cgcacgcaca cgcgcttcaa actgagccaa gccatctgcc
    10441 tctaaaaagc ttttcaagtt ctcacgacta aagaaaatag gattaaaaat tccttggcaa
    10501 atcgtaaaac gagctacagg gtctgctaga taggcttgaa tacttgcttg gaaataagct
    10561 tcaaaatcaa aaccatctag tccttcaata tcagtccctg tataagcaag caaagcatct
    10621 aaaaatgact tgataatctg ccagtctgtc tttgttagta ggtcaggaac atcgacacga
    10681 taagcctttt gaccatcagc ataactgacc ttaaagagaa attgtgattg ccccactatc
    10741 gcacactcga tatattcgag acgattaaga ggctgacgga gatagactac atcatagcta
    10801 tgtgactcca aaccgtctag caaggccaag atcgacttgg cattcaaaat ctcttgttct
    10861 cctaaaatgc tctgtttatt tggaataaaa aatgttttcg ccataactgg actcctttga
    10921 aatcgtttac atacagtata ccttattttt ctgaaagata caaaaacaaa ctttagattt
    10981 ttgagtacaa agcatagaaa aacagcccct gaggactgtt tgatcttata cagtagctgc
    11041 ttgatccaag ctttcaccga tagcggctag gcgctcgatc acttcagctt gtgtcaattc
    11101 attttttgaa acatagcggt tacgtgggtg aacacggcac tcgtgtgagc atccacgaag
    11161 gtacttgtct tcattttctt ctgatgtcaa gatacgacgg ttacagaagg gatttccaca
    11221 gttgacataa cgttcacatg gtgttccatc aaaccagtct ttccctacga tagttgggtt
    11281 gacatggttg acatcaacgg caatacgctc gtcaaagacg tacattttcc catcccaaag
    11341 ctcaccttga acttctgggt ctttaccgta agttgcgatt cctccgtgca attggccgac
    11401 atctttgtaa ccttcacgaa ccatccagcc tgagaatttc tcacagcgaa cgccacctgt
    11461 acagtaaacc acgacacgct tgtccatgaa tttttccttg ttatcacgga cccattgtgg
    11521 taactcacgg aagttgcgaa tatctgggcg aatagcccca cggaaatgtc ctaggtcata
    11581 ctcataatca ttacgtgtgt caaggacaac ggtatcttta tcaagaagcg cttctttgaa
    11641 ctcttttgga gacaagtaag cacctgttgt ttcaagtggg ttgatgtcat tgtcaaagtc
    11701 gttgtcttcc aaaccaaggt ggacaatttc tttcttgtag cgaacaaaca tcttcttgaa
    11761 ggcttgttca ctttcttcgt caatcttgaa ccagagttct tccattcctg gaaggctgtg
    11821 aacgtagtcc atgtattttt gagttgtttc atagtcacct gaaactgttc cgttaattcc
    11881 ctcgtcagcg actaggatac gtcctttaag accgattgat ttacagaaag ccaagtggtc
    11941 tgcagcaaat tgctctgcat tttcaattgg agtataaagg tagtaaagta agacacgaat
    12001 atcttttgcc ataagatttg ttctcttttc tattcttaaa ttttctaaat tttttattta
    12061 tctacactag tatacctgct tgagaaaacg aatgcaattt atttgactgg aaattgtaga
    12121 gggttctcga aatttcctga atggttaaaa taaggacaag agaaaacatg gatatctata
    12181 tccttgtgcc aaaaaaacca ctgtctcccc cagaccaacc tgaggaaagc agtgattctt
    12241 attttaggag ttaggaatga atacacgaaa tcaatttagc tgattatttt ttgtttttca
    12301 agaattcatc gtattgtttt tgcatttcgt tcaatacttt ttcgtaggca ccttcagatt
    12361 tcaatttttc catcaattct ggaatcgctt tatctgggtc tacagtacca gtgttgatag
    12421 ctgtatcaaa ttgttgcatt gtgttagcaa tagctgagat ttcagatttc acattgtcag
    12481 tattgaagat aaatccaagc gctggagatt ctttagcttc tgccaattct ttcttagaat
    12541 tttcgatttg ttggtctgta acgttttcgt tgatgtaaag gatccagttg ttaccagtgt
    12601 tccatccacc catgtgagtg tttcctttgt agccatcaag aacgcgaaca cggttttctt
    12661 taccttcaat tttttcccag ttcttgcctt ctggaccgta aacaagaccg ttcaagagtt
    12721 ctgggttcgt attcaagagg ttcaagattt ccattgattt ttctttgttc ttagagttgt
    12781 ttgagatgac aaagttagca acttgtgttg tttggttttt cttgatgaag ttagtaattg
    12841 gtttgatttg gatatctttg ttggcaacac gtgaaagcaa gctgttaccg tagtcagctg
    12901 gtcctactgt ttcttcacga acgaaccaag tatcttgttg aaggtcaaat gaagtgtcac
    12961 ttgttgcgac gtcttttgga atgtagccag cttcatagaa tttgtgaaga gtcttcaagt
    13021 gttctttgaa acgaggcact tcgtaacggt ttacaacttt agtagtatcg ccttcaaggt
    13081 caataacgaa tggaagcccg tttgctactg ggtagtcaaa gttatcagat gggatgaaaa
    13141 ctttaccaat agcaaatggt actacgtctg gagctttttc tttgatttgt ttcaagactg
    13201 gctcaagagt ttcgtaagaa gtaacacctg aaatatcgat accatatttg gcaaggagag
    13261 ttccgttgaa ggcaaagttt tgagatgatg ctacgttggc tgcaactggt acagcgtaaa
    13321 tcttaccatt tacagtatta cccttgatat aagctggatc aagtgccttg taaaggtctt
    13381 taccttcttt tttgtacaat tctgtcaagt cagcataagc acctttttga gcatttacaa
    13441 tatagttatc tgcaaaggcg atatcatagt tttcaccaga tgatgtgata actgacattt
    13501 tcttaccata gtcaccccag ccaaggtatt ggatatccaa tttggcacca actttttctt
    13561 cgatgatttt gttggcattt gctaacaatt catccaagtt atctggtttg tcaccgattt
    13621 ggtacatttt gataacagat ttgtcacctg aatcagcagc ttttttgctg ttacctgtca
    13681 agttgccaca agcagcaaga cctgcagcca aagcgactac actagcagat gcaaaagcat
    13741 attttttcca atttttcatg ataaaaactc ctttttttat ttttaaactt ataaacaatg
    13801 taatgatctt aacttcacgc aagggaagtt tggaaatgag aaatgttctt tttcaataag
    13861 cactattctt tcacaccacc gatagtcaaa ccttttacaa agtagcgttg gaagaatgga
    13921 tacaaaatcg cgattggaag ggttgctacc acaaccatgg ccatacgacc tgtttctttc
    13981 ggtagagcaa ctcccagttg accagtcaag ccgaccgctt tggcaatgta gtccatattt
    14041 tgttggattt gcatgagcaa atattgcaat ggatacaagt tatcactctt gatgtaaaga
    14101 agggcgttga accagtcatt ccagaaacca agagctgtca aaagcgtgat ggttgcgata
    14161 cctggtagtg acaatggcaa acagatttgg aagaaaatcc gagcctcact ggcaccatcg
    14221 atacgagccg attcgagaat ggcttctgga atggtcttct tgaaaaaaga acgcatcaag
    14281 atgatattga atggtgagag aagcattgga acaatcaagg cccaaacagt gtcaccaagc
    14341 tgaagtacac gggtcaccat gatataacct ggtaccaaac cagcgttgaa caacatactg
    14401 agaaggacga agatggtaaa aaatctgcga tacttaaagg ttgtccgtga aatagcgtag
    14461 gcataggttg ttgtgataaa gacatttgtc aatgtcccaa ctacggttac aaagacagag
    14521 atgaagaggg cttgtaggat tttatcctta aactgtgcca aaaactcaaa accgtctaag
    14581 ccaaattggg atgggaagaa gctatagccg tattggagga ggcttttctc gtctgtcact
    14641 gaaataatga taacgaatac aaaaggtagg atacaagaga gggcaatcaa acccgaaatg
    14701 atactgaaga agatatctgc tttcttactg aaggagtgaa tgccgacatt atcaattttt
    14761 tcttttttaa ttttcttttt tgccatattc tcctcctttc tagaacaaag ctgagtttgg
    14821 atcgactcgt cttgcaagca agtttgatag gataaccaga atcaaaccaa caacggattg
    14881 gtaaagaccg gctgctgcag ccataccgat atctgctgtc tgagtcaaac cattaaagac
    14941 atatacgtcc aaaacgttgg ttacattgta aagctgacca gcattgtgtg ggatttgata
    15001 gaagagaccg aagtctgcgc ggaagatatt tccgactgca aggatggtca atacagttac
    15061 aagcggagtc aactgaggaa tggttacatt gcgaatacgt tgccacttgc tagctccgtc
    15121 cactgtcgct gcttcgtagt aggttggatc aattcccatg atcgtcgcat agtacatgac
    15181 actgctatat ccaaagcctt tccaaatacc taggaaaagt aggagatagg gccagatgcc
    15241 caggtcagcg tagaaattga cttctttgag accaagactt tccaatagat gattgaacac
    15301 ccctttatca atatttagga aggcatctgt aaagaaactg atgataaccc aagacaagaa
    15361 gtaagggaac aacatagaag tttgaaaaat cttcaccatt ctcttagaac ggagctcgct
    15421 gaggataatg gcaatcccta cagatacaac taaacctaga aagataaagc caagattgta
    15481 gaggacagta tttcgtgtga taataaaggc gtctcttgaa ctaaataaga atctgaaatt
    15541 atcgagtccg acccatttac tatttatgat actatctatg aaaccattac tggtcatgtg
    15601 gtagtctttg aaggcaacca cgttcccaaa tactggaatg taaaagaata gaatcaacca
    15661 gagtgcccct ggcaaaacca tcaagagaaa gatccagttg tctctcaatg tttttgaaaa
    15721 ctttttcata atttcctccc tttttatttt gatatccatc taaaaattct tttttagact
    15781 tttgataacg attacattat tagtatactc ctatttgcag gttaggttaa actcctaatt
    15841 atagaaaaaa ctccacaaat tatgtagcag atttaaaact ttatcaccac tatcaaacaa
    15901 atgtcctaaa tcaattgttt attttatctc tattagccca gtgatggcgt cactctgtta
    15961 taagcatcca acaacggggt atactgaaaa atctccagac tagggaactc agcgatagtt
    16021 cctaatctgg agatttttaa tatgttatta ggcgtttgct ttcaacttag caataacctc
    16081 tttaagatta tcaatcaact ctgctgcagt atgctcagag cctttttcat ctgccaagaa
    16141 caaaactgct ttttgaagtt cttcttgaga gttttcaagg acatccttat ctactgtttc
    16201 aaggtttgag tctttaagaa gtttacttaa ttccttggct aatttcttga gtttgatttg
    16261 cagactcatc ttctcctgct gtttctttgc ccgctgtttg tcctccatcc ttagttgctg
    16321 actggctttc cttaatggac tctagggaag caatggcatc tttgactgtt tgcaagatat
    16381 cacgtaaacc ttgctctatc aaactatcat ctgcaaaagc tttcttagag tctgccaaaa
    16441 ccagacgtgc tgaatctgtg gtaggattcg atacacctgt caatgatctc aaaagatttt
    16501 ctaaggtttg agtctgctta ctaatactag actaaaatca aaaagcatta tataacagtg
    16561 atatgaaatc aact
//
LOCUS       DAVLVP010000038        16200 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.038, whole genome shotgun sequence.
ACCESSION   DAVLVP010000038 DAVLVP010000000
VERSION     DAVLVP010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 16200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16200
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.038"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            128..2293
                     /locus_tag="VYN69_001171"
     CDS             128..2293
                     /locus_tag="VYN69_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1533450.1"
                     /translation="MKKKFLAFLLILFPIFSLGIAKAETIKIVSDTAYAPFEFKDSDQ
                     TYKGIDVDIINKVAEIKGWNIQMSYPGFDAAVNAVQAGQADAIMAGMTKTKEREKVFT
                     MSDTYYDTKVVIATTKSHKISKYDQLTGKTVGVKNGTAAQRFLETIKDKYGFTIKTFD
                     TGDLMNNSLSAGAIDAMMDDKPVIEYAINQGQDLHIEMDGEAVGSFAFGVKKGSKYEH
                     LVTEFNQALAEMKKDGSLDKIIKKWTASSSSAVPTTTTLAGLKAIPVKAKYIIASDSS
                     FAPFVFQNSSNQYTGIDMELIKAIAKDQGFEIEITNPGFDAAISAVQAGQADGIIAGM
                     SVTDARKATFDFSESYYTANTILGVKESSNIASYEDLKGKTVGVKNGTASQTFLTENQ
                     SKYGYKIKTFADGSSMYDSLNTGAIDAVMDDEPVLKYSISQGQKLKTPISGTPIGETA
                     FAVKKGANPELIEMFNNGLANLKANGEFQKILDKYLASESSTASTSTVDETTLWGLLQ
                     NNYKQLLSGLGITLALALISFAIAIVIGIIFGMFSVSPYKSLRVISEIFVDVIRGIPL
                     MILAAFIFWGIPNFIESITGQQSPINDFVAGTIALSLNAAAYIAEIVRGGIQAVPVGQ
                     MEASRSLGISYGKTMRKIILPQATKLMLPNFVNQFVIALKDTTIVSAIGLVELFQTGK
                     IIIARNYQSFKMYAILAIFYLVIITLLTRLAKRLEKRIR"
     gene            2293..3033
                     /locus_tag="VYN69_001172"
     CDS             2293..3033
                     /locus_tag="VYN69_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1533451.1"
                     /translation="MAKLKIDVNDLHKHYGKNEVLKGITTKFYEGDVVCIIGPSGSGK
                     STFLRSLNLLEEVTSGHITVNGYDLTEKTTNVDHVRENIGMVFQHFNLFPHMSVLDNI
                     TFAPIEHKLMTKEEAEELGMELLEKVGLADKANANPDSLSGGQKQRVAIARGLAMNPD
                     IMLFDEPTSALDPEMVGDVLNVMKELAEQGMTMIIVTHEMGFARQVANRVIFTADGEF
                     LEDGTPDQIFDNPQHPRLKEFLDKVLNV"
     gene            3182..4669
                     /locus_tag="VYN69_001173"
     CDS             3182..4669
                     /locus_tag="VYN69_001173"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000096131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEV1533452.1"
                     /translation="MSSKVIVTIFGASGDLAKRKLYPSLFRLYQSGNLSKHFAVIGTA
                     RRPWSKEYFESVVVESILDLADSTEQAQEFASHFYYQSHDVNDSEHYIALRQLQAELN
                     EKYQAEHNKLFFLSMAPQFFGTIAKHLKSENIVDGKGFERLIVEKPFGTDYATASKLN
                     DELLATFDEEQIFRIDHYLGKEMIQSIFAVRFANLIFENVWNKDFIDNVQITFAERLG
                     VEERGGYYDQSGALRDMVQNHTLQLLSLLAMDKPASFTKDEIRAEKIKVFKNLYHPTD
                     EELKEHFIRGQYRSGKIDGMKYISYRSEPNVNPESTTETFTSGAFFVDSDRFRGVPFF
                     FRTGKRLTEKGTHVNIVFKQMDSIFGEPLAPNILTIYIQPTEGFSLSLNGKQVGEEFN
                     LAPNSLDYRTDATATGASPEPYEKLIYDVLNNNSTNFSHWDEVCASWKLIDRIEKLWA
                     ENGAPLHDYKAGSMGPQASFDLLEKFGAKWTWQPDITYRQDGRLE"
     gene            complement(4717..5994)
                     /gene="ftsY"
                     /locus_tag="VYN69_001174"
     CDS             complement(4717..5994)
                     /gene="ftsY"
                     /locus_tag="VYN69_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000522312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEV1533453.1"
                     /translation="MGLFDRLFGKKEEPKIEEVVKEALENLDLSEDVDPTFTEVEEVS
                     QEEAEVEIVEQAVFQEEEIQDTVEESLDLEPVVEVSQKEVEEFPHSEEGNTEFLATVE
                     ENNSEILEAERPQTEETVQEKYDRSLKKTRTGFGARLNAFFANFRSVDEEFFEELEEL
                     LIMSDVGVQVASNLTEELRYEAKLENAKKPDALRRVIIEKLVELYEKDGSYDESIHFQ
                     DNLTVMLFVGVNGVGKTTSIGKLAHRYKRAGKKVMLVAADTFRAGAVAQLAEWGRRVD
                     VPVVTGPEKADPASVVFDGMERAVAEGIDILMIDTAGRLQNKDNLMAELEKIGRIIKR
                     VVPEASHETFLALDASTGQNALVQAKEFSKITPLTGIVLTKIDGTARGGVVLAIREEL
                     NIPVKLIGFGEKIDDIGEFNSENFMKGLLEGLI"
     gene            complement(5998..6816)
                     /locus_tag="VYN69_001175"
     CDS             complement(5998..6816)
                     /locus_tag="VYN69_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1533454.1"
                     /translation="MADIKLIALDLDGTLLTTDKRLTDRTKATLKAARDRGIKVVLTT
                     GRPLKAMDFFLHELGTDDHEDEYTITFNGGLVQKNTGEILDKTVFSYDDVARLYEETE
                     KLSLPLDAISEGTVYQIQSDQESLYAKFNPALTFVPVDFADLSSQMTYNKCVTAFAQE
                     PLDAAIQKISPELFDQYEIFKSREMLLEWSPKNVHKATGLAKLISHLGIDQSQVMACG
                     DEANDLSMIEWAGLGVAMQNAVPEVKAVANVVTPMTNDEEAVAWAIEEYVLKEN"
     gene            complement(6816..7610)
                     /locus_tag="VYN69_001176"
     CDS             complement(6816..7610)
                     /locus_tag="VYN69_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEV1533455.1"
                     /translation="MTIKLVATDMDGTFLDGNGRFDMDRLKSLLASYKEKGIYFAVAS
                     GRGFLSLEKLFADVRDDIIFIAENGSLVEYQGQDLYEATMSRDFYLATFEKLKTSPYI
                     DINKLLLTGKKGSYVLDTVDETYLKVSQHYNENIQKVASLEDITDDIFKFTTNFTEET
                     LEAGEAWVNDNVPGVKAMTTGFESIDIVLDYVDKGVAIVELAKKLGITMDQVMAFGDN
                     LNDLHMMQVVGHPAAPENARPEILELAKTVIGHHKDQSVIAYMEGL"
     gene            complement(7607..11146)
                     /gene="smc"
                     /locus_tag="VYN69_001177"
     CDS             complement(7607..11146)
                     /gene="smc"
                     /locus_tag="VYN69_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000280891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEV1533456.1"
                     /translation="MYLKEIEIQGFKSFADKTKVVFDQGVTAVVGPNGSGKSNITESL
                     RWALGESSVKSLRGGKMPDVIFAGTESRKPLNYASVVVTLDNHDGFIKDAGQEIRVER
                     HIYRSGDSEYKIDGKKVRLRDIHDLFLDTGLGRDSFSIISQGKVEEIFNSKPEERRAI
                     FEEAAGVLKYKTRRKETESKLQQTQDNLDRLEDIIYELDNQIKPLEKQAENARKFLDL
                     EGQRKAIYLDVLVAQIKENKAELESTEEELAQVQELLMSYYQKREKLEEENQTLKKQR
                     QDLQAEMAKDQGSLMDLTSLISDLERKLALSKLESEQVALNQQEAQARLAALEDKRNS
                     LSKEKSDKESSLALLEGNLVQNNQKLNRLEAELLAFSDDPDQMIELLRERFVALLQEE
                     ADVSNQLTRIENELENSRQLSQKQADQLEKLKEQLAIAKEKASQQKEELETAKEQVQK
                     LLADYQAIAKEQEEQKTSYQAQQSQLFDRLDNLKNKQARAQSLENILRNHSNFYAGVK
                     SVLQEKDRLGGIIGAVSEHLTFDMHYQTALEIALGASSQHIIVEDEESATKAIDFLKW
                     NRAGRATFLPLTTIKARTISSQNQDAIAVSPGFLGMADELVTFDTRLEAIFKNLLATT
                     AIFDTVEHARAAARQVRYQVRMVTLDGTELRTGGSYAGGANRQNNSIFIKPELEQLQK
                     EIAEEEASLGSEEAALKTLQDQMAALTERLEAIKSQGEQARIQEQGLSLAYQQTSQQV
                     EELETLWKLQEEEIDRLSEGDWQADKEKCQESLATIASEKQNLEAEIEEIKSNKNAIQ
                     ERYQNLQEEVAQARLLKTELQGQKRYEVADIERLGKELDNLNIEQEEIQRMLQEKVDN
                     LEKVDTELLSQQAEESKTQKTNLQQGLIRKQFELDDIEGQLDDIASHLDQARQQNEEW
                     IRKQTRAEAKKEKVSERLRHLQNQLTDQYQISYTEALEKAHELENLNLAEQEVQDLEK
                     AIRSLGPVNLEAIDQYEEVHNRLDFLNSQRDDILSAKNLLLETITEMNDEVKERFKST
                     FEAIRESFKVTFKQMFGGGQADLILTEGDLLTAGVEISVQPPGKKIQSLNLMSGGEKA
                     LSALALLFSIIRVKTIPFVILDEVEAALDEANVKRFGDYLNRFDKDSQFIVVTHRKGT
                     MAAADSIYGVTMQESGVSKIVSVKLKDLESIEG"
     gene            complement(11137..11835)
                     /locus_tag="VYN69_001178"
     CDS             complement(11137..11835)
                     /locus_tag="VYN69_001178"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEV1533457.1"
                     /translation="MKELQTVLKNHFEIEFADKKLLETAFTHTSYANEHRLLKISHNE
                     RLEFLGDAVLQLLISEYLYKKYPKKPEGDLSKLRAMIVREESLAGFARDCQFDQFIKL
                     GKGEEKSGGRNRDTILGDAFEAFLGALLLDKDVAKVKEFIYQVMIPKVEAGEFEMITD
                     YKTHLQELLQVNGDVAIRYQVISETGPAHDKVFDVEVLVEGKSIGQGQGRSKKLAEQE
                     AAKNAVEKGLDSCI"
     gene            complement(12018..12089)
                     /locus_tag="VYN69_001179"
     tRNA            complement(12018..12089)
                     /locus_tag="VYN69_001179"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12054..12056),aa:Arg,seq:ccg)
     gene            complement(12153..13139)
                     /locus_tag="VYN69_001180"
     CDS             complement(12153..13139)
                     /locus_tag="VYN69_001180"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEV1533458.1"
                     /translation="MLNEFPIFDYEDIQLIPNKCVIKSRAEADTSVTLGNHTFKLPVV
                     PANMQTILDENVAEQLAKGGYFYIMHRFDEAGRIPFIKRMHDQGLIASISVGVKDYEY
                     DFVSQLKADAPEYITIDIAHGHADSVISMIQHIKKELPDTFVIAGNVGTPEAVRELEN
                     AGADATKVGIGPGKVCITKVKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIA
                     KSIRFGASMIMIGSLFAGHIESPGKTIEVDGEQFKEYYGSASQYQKGAYKNVEGKRIL
                     LPAKGHLQDTLTEMEQDLQSAISYAGGRQVADLKHVDYVIVKNSIWNGDASH"
     gene            complement(13314..14630)
                     /locus_tag="VYN69_001181"
     CDS             complement(13314..14630)
                     /locus_tag="VYN69_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001223073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrC family protein"
                     /protein_id="HEV1533459.1"
                     /translation="MRITDNQHKIIKEKFVEEYPKLSNLLLDRTLESLSQDERIFIFP
                     NDLTHTPDLDKKQKILETINQEIKTGNVIGFLGYGQERLTISSRFSDESNDHFLHYLL
                     NKVLHINLTSLDVALSREERLYQLLMYLFPKYLQAAIRKGLYKEYHRFSHNDSHVKGV
                     IDVRNHLKKNLPFTGNIAYTTREFTYDNPLMQLVRHTIEYIKNQKSIGQGVLDNLSTS
                     RENVSEIVRVTPSYKLADRAKITRRNQSKPIRHAYFHEYRNLQELCLMILNQEKHGLG
                     YQDQKIYGILFDVAWLWEEYVYTLLPKGFVHPRNKDKTDGISVFSVGKRKVYPDFYDR
                     ERKIVLDAKYKKLELTEKGINREDLFQLISYSYILKAEKAGLVFPSKDKVIDNEMGNL
                     AGYGAQLKKWSIRIPQNASFYSAFCKMMENSEENFKAIIDEEVGRK"
     gene            complement(14617..>16200)
                     /locus_tag="VYN69_001182"
     CDS             complement(14617..>16200)
                     /locus_tag="VYN69_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="McrB family protein"
                     /protein_id="HEV1533460.1"
                     /translation="RFSKLKSDLYEIIKEGIRFNFENSIFDMKRSTNEFIGRSAMVTK
                     LLCIYSEGDPFFGVNINSQKEFWNHFVSQTNQGGPYLQNHKIIELVSKTYPELEPSKL
                     GTMLFEYSKLFMENKEDNSTMDSSNNFRHQLTQSLLKSPNLILRGAPGTGKTYLAKEI
                     AKELTDGNEDQIGFVQFHPSYDYTDFVEGLRPVSNGDGAIEFRLQDGIFKDFCQKAKE
                     TQLIGGQDNFDEAWDSYLEYINVAEEKEYITKTSYLSVNSRQNLSVNYDSGVPGWSLP
                     SKYVYELYKDKNYNKQEYYKSGGKTVLETLRKRFGLKDYVSPTEIDTDKKFVFIIDEI
                     NRGEISKIFGELFFSIDPGYRGEKGSVSTQYANLHETDEKFYIPENVYIIGTMNDIDR
                     SVDTFDFAMRRRFRFVEVTAEGQVGMLDKELNIHAEEAKIRLRNLNAAIENVQELNSH
                     YHIGPSYFLKLKDVDFDYELLWSDYIKPLLEDYLRGSYDEVETLETLKKAFELTNNEQ
                     KDQAVADDNESDENDDADY"
BASE COUNT         4663 a         3584 c         2914 g         5039 t
ORIGIN      
        1 ctttttaata gcaattcaag gaaaggaaaa aatcgatgtt atattttctg acacgaaaaa
       61 tataaaaatt tgcctttttt tactttttct gatataatga gagaatattc ggaaaaggag
      121 actaaaaatg aagaaaaaat ttctagcatt tttgctaatt ttattcccaa ttttctcatt
      181 aggtattgcc aaagcagaaa cgattaagat tgtttctgat accgcctatg caccttttga
      241 gtttaaagat tcagatcaaa cttataaagg aattgatgtt gacattatta acaaagtcgc
      301 tgagattaaa ggctggaaca ttcagatgtc ctatcctgga tttgacgcag cagtcaatgc
      361 ggttcaagct gggcaagccg acgctatcat ggcagggatg acaaagacta aagaacgtga
      421 aaaagtcttc accatgtctg atacttacta tgatacaaaa gttgtcattg ctactacaaa
      481 gtcacacaaa attagcaagt acgaccaatt aactggcaaa accgttggtg ttaaaaacgg
      541 aactgccgct caacgtttcc ttgaaacaat caaagataaa tacggcttta ctattaaaac
      601 atttgacact ggtgatttaa tgaacaacag cttgagtgct ggtgccatcg atgccatgat
      661 ggatgacaaa cctgttatcg aatatgccat taaccaaggt caagacctcc atattgaaat
      721 ggatggtgaa gctgtaggaa gttttgcttt cggtgtgaaa aaaggaagta aatacgagca
      781 cctggttact gaatttaacc aagccttggc tgaaatgaaa aaagatggta gtcttgataa
      841 aattatcaag aaatggactg cttcatcatc ttcagcagtg ccaactacaa ctactctcgc
      901 aggattaaaa gctattcctg ttaaggctaa atatatcatt gccagcgatt cttcttttgc
      961 accttttgtt ttccaaaatt caagcaacca atacactggt attgatatgg aattgattaa
     1021 ggcaatcgct aaagaccaag gttttgaaat tgaaatcacc aaccctggtt ttgatgctgc
     1081 tatcagtgct gtccaagctg gtcaagccga tggtatcatc gctggtatgt ctgtcacaga
     1141 tgctcgtaag gcaacttttg acttctcaga atcatactac actgctaata ccattcttgg
     1201 tgtcaaagaa tcaagcaata ttgcttctta tgaagatcta aaaggaaaga cagtcggtgt
     1261 taaaaacgga actgcttctc aaaccttcct aacagaaaat caaagcaaat acggctacaa
     1321 aatcaaaacc tttgctgatg gttcttcaat gtatgacagt ttaaacactg gtgccattga
     1381 tgccgttatg gatgatgaac ctgttctcaa atattctatc agccaaggtc aaaaattgaa
     1441 aactccaatc tctggaactc caatcggtga aacagccttt gccgttaaaa aaggagcaaa
     1501 tccagaactg attgaaatgt tcaacaacgg acttgcaaac cttaaagcaa acggtgaatt
     1561 ccaaaagatt cttgacaaat acctagctag cgaatcttca actgcttcaa caagtactgt
     1621 tgacgaaaca acgctctggg gcttgcttca aaacaactac aaacaactcc ttagcggtct
     1681 tggtatcact cttgctctag ctcttatctc atttgctatt gccattgtca tcggaattat
     1741 cttcggtatg tttagcgtta gcccatacaa atctcttcgc gtcatctctg agattttcgt
     1801 tgacgttatt cgtggtattc cattgatgat tcttgcagcc ttcatcttct ggggaattcc
     1861 aaacttcatc gagtctatca caggccaaca aagcccaatt aacgactttg tagctggaac
     1921 cattgccctc tcactcaatg cggctgctta tatcgctgaa atcgttcgtg gtggtattca
     1981 ggccgttcca gttggccaaa tggaagccag ccgaagcttg ggtatctctt atggaaaaac
     2041 catgcgtaag attatcttgc cacaagcaac taaattgatg ttgccaaact ttgtcaacca
     2101 attcgttatc gctcttaaag atacaactat cgtatctgct atcggtttgg ttgaactctt
     2161 ccaaactggt aagattatca ttgctcgtaa ctaccaaagt ttcaagatgt atgcaatcct
     2221 tgctatcttc tatcttgtaa ttatcacact tttgactaga ctagcgaaac gcttagaaaa
     2281 gaggattcgt taatggcaaa attaaaaatt gatgtaaatg atttacacaa gcactatgga
     2341 aaaaatgaag tcctaaaagg aattacgact aagttctatg aaggagatgt tgtttgtatc
     2401 atcggtcctt caggttctgg taagtcaact ttcctccgta gcctcaatct tttagaagaa
     2461 gtcactagcg gtcacatcac tgtgaacggc tatgatttaa ctgaaaaaac aaccaatgtt
     2521 gaccacgtcc gtgaaaatat cggcatggta ttccaacact tcaacctctt ccctcatatg
     2581 tctgtattgg acaacatcac ctttgctcct attgagcaca agttgatgac taaggaagaa
     2641 gctgaggaat tgggaatgga gttgcttgaa aaggttggac tagcagataa agctaatgcc
     2701 aatccagata gcctatcagg tggtcaaaaa caacgtgtgg ccatcgctcg tggcctagca
     2761 atgaatccag acatcatgct cttcgatgaa ccaacttctg cccttgaccc tgagatggtt
     2821 ggagacgtac ttaacgttat gaaggaattg gctgagcaag gcatgaccat gattatcgta
     2881 acccatgaga tgggatttgc tcgtcaggtt gccaaccgcg ttatctttac tgcagatggc
     2941 gagttccttg aagacggaac acctgaccaa atctttgata acccacaaca ccctcgtctg
     3001 aaagagttct tagataaggt cttaaacgtc taaactcaaa ctgtaaggat ttccttgcag
     3061 tttttctacc tcgtattgga atttttgatt tttcggaaaa ttatgttaga attaagttta
     3121 tgaaatgagg tttcctcata cctagcaaga ctaggaataa aaatagaaat taggtagcta
     3181 gatgtcatct aaggttattg ttacaatttt cggtgcgagt ggagacctgg ctaaacgcaa
     3241 gctctaccct tcccttttta gactatatca atccggcaat ctttccaagc actttgccgt
     3301 tattggaact gcccgtagac cttggagtaa ggaatatttt gaatctgtag ttgtcgagtc
     3361 catccttgat ttggcagata gtaccgagca agcccaagaa tttgctagcc acttctacta
     3421 tcaaagccat gatgtcaatg attcggaaca ttatattgct ttgcgtcaat tacaagctga
     3481 gcttaatgaa aaataccaag ctgaacacaa taagctcttc ttcttgtcta tggcacctca
     3541 gttctttgga accattgcca aacacctcaa atctgaaaac attgtcgatg gcaaaggttt
     3601 tgagcgcttg atcgttgaaa aaccatttgg tacagattac gcaactgcaa gcaagttgaa
     3661 tgacgaactc ctagcaacat ttgacgaaga acaaattttc cgtatcgacc attatcttgg
     3721 taaggaaatg atccaaagca tctttgcagt tcgctttgca aacttgattt ttgaaaacgt
     3781 ttggaacaag gattttatcg acaatgttca aattaccttt gcggagcgct tgggtgtaga
     3841 agaacgtggt ggctactatg accaatccgg tgccctccgt gacatggtcc aaaaccacac
     3901 tctacaactt ctttcgctcc tcgccatgga caaaccagca agcttcacaa aagacgagat
     3961 tcgtgctgaa aagattaagg tctttaaaaa cctctatcat ccaactgatg aagaactcaa
     4021 agaacacttt atccgtgggc aataccgctc tggtaagatt gatggcatga aatacatctc
     4081 ttatcgtagc gagccaaatg tgaatccaga atcaacaact gaaaccttta catctggtgc
     4141 cttctttgta gacagcgatc gattccgtgg tgttcctttc tttttccgta caggtaaacg
     4201 actgactgaa aaaggaactc atgtcaacat cgtctttaaa caaatggatt ctatctttgg
     4261 agaaccactt gctccaaata ttttgaccat ctatattcaa ccaacagaag gcttctctct
     4321 tagcctaaat gggaagcaag taggagaaga atttaacttg gctcctaact cacttgatta
     4381 ccgtacagat gcgactgcaa ctggtgcttc tccagaacca tacgaaaaat tgatttatga
     4441 tgtcctaaat aacaactcaa ctaactttag ccactgggat gaagtttgtg cgtcatggaa
     4501 gttgattgac cgtattgaaa agctctgggc tgaaaatggt gccccacttc atgactataa
     4561 agctggaagc atgggacctc aagccagctt tgacctactt gaaaaattcg gtgccaaatg
     4621 gacttggcaa ccagatatca cctatcgtca agatggtcgc ttagaataaa aaaatttcct
     4681 gcaagtttat gccttgcagg attttttagc ttctgattag attaaacctt ccaagagacc
     4741 tttcatgaag ttttctgagt taaactctcc aatatcatcg attttttcac caaaaccaat
     4801 caattttaca ggaatattga gttcttcacg aatggctaga accacacctc ctcgagcagt
     4861 tccatcaatc ttagtcaaaa caattcccgt taaaggtgtg attttcgaaa attctttggc
     4921 ctgtactagg gcattttgac ctgttgatgc atcaagtgcc aagaaggttt catgtgatgc
     4981 ttctggcaca acacgtttga taatacgacc aatcttttcc aactcagcca taaggttatc
     5041 cttattttgc agacgaccag cagtatcaat catgagaata tcgatacctt cagccacggc
     5101 acgttccata ccatcaaaga ccacgctggc tggatcagct ttttcaggtc cagttactac
     5161 tggaacatct actcgtcggc cccattcagc tagctgagct actgcacccg cacggaaggt
     5221 atctgctgca accagcatga ccttcttacc agctcgtttg tagcggtggg ctagttttcc
     5281 gatagaagtt gttttcccaa caccattcac accaacaaag agcataactg tcaagttatc
     5341 ttggaagtgg atgctttcat cgtagctacc atccttttca taaagctcaa ccaatttctc
     5401 aatgatgaca cgacgaagtg catcaggttt cttggcattt tcaagcttgg cttcgtaacg
     5461 tagttcctcc gttaagttag aagcgacttg gacaccaaca tcactcataa tcagcagttc
     5521 ttccagttcc tcgaaaaatt cttcgtcaac agagcggaag ttagcaaaga aggcattcaa
     5581 gcgggcaccg aaacctgtgc gagttttctt aagactgcgg tcatattttt cctgaacagt
     5641 ttcttctgtt tgaggccttt ctgcttcaag aatttcagaa ttattttctt ctacagtcgc
     5701 tagaaactca gtattccctt cttctgagtg tggaaattct tcgacttctt tttgagaaac
     5761 ttctacaact ggctctaaat ccagactttc ttcaactgtg tcttggattt cctcttcttg
     5821 gaacacagct tgttcaacaa tttcaacctc tgcttcttcc tgagaaactt cctcaacttc
     5881 tgtgaaggta ggatcaacat cttcagacaa atcaagattt tccagagctt cttttacaac
     5941 ttcttcgatt ttaggttctt ctttttttcc gaatagacgg tcaaacaatc ccatatctta
     6001 gttctccttt agcacgtatt cttcgatagc ccaggcgaca gcttcctcat cgttggtcat
     6061 tggcgtcact acatttgcga cagccttgac ttcaggaaca gcgttttgca tagcaacacc
     6121 gagccctgcc cattcaatca tagagaggtc attggcttca tcaccacagg ccatcacttg
     6181 actttgatca attccaagat ggctgatcag ttttgccaag cctgttgctt tatgaacatt
     6241 ctttggtgac cactctagca acatttcacg tgatttaaag atttcatact ggtcaaacaa
     6301 ttctggagaa atcttctgaa tggctgcatc caagggttct tgagcaaagg cagtcacgca
     6361 tttgttgtag gtcatttgac tagacagatc tgcaaagtcc actggaacaa aggtcaaagc
     6421 tggattgaat ttggcataaa gactttcttg gtccgattga atttgataaa cagttccttc
     6481 tgagatagcg tcaagaggca gtgataattt ctctgtttct tcatacaaac gtgccacatc
     6541 atcatatgaa aagactgttt tatcaaggat ttctcctgta tttttctgaa ctaatccacc
     6601 attaaaagta atggtatact catcttcgtg gtcgtcagtc cctaactcat ggagaaagaa
     6661 gtccatggct tttaagggac gaccagttgt caatacgacc ttgataccac gatcacgcgc
     6721 agctttcaaa gttgccttgg tgcgatccgt cagcctttta tcagtagtca gcaaggtacc
     6781 gtccaagtcc aatgcgatta attttatatc tgccattata agccctccat ataagctata
     6841 accgactggt ccttatggtg accaatcaca gtctttgcta attctaaaat ctctggtcgt
     6901 gcattttcag gagctgcagg atgtcccaca acctgcatca tatgtaagtc attaagattg
     6961 tctccaaaag ccatgacctg atccattgtg atgccaagtt ttttagctaa ttcaacaata
     7021 gctacaccct tatcgacata gtccagaaca atatcaatag attcaaagcc agttgtcata
     7081 gccttgacac cagggacatt atcattgacc caagcttcac cagcttctag cgtttcttct
     7141 gtgaagttgg ttgtaaattt gaaaatgtca tctgtgatat cttccaaact cgctactttt
     7201 tggatatttt cattataatg ctgactcact ttcaaatagg tctcatcaac cgtatctaga
     7261 acatatgaac ccttcttacc cgtcaagagc agtttattga tatctatata aggtgacgtt
     7321 ttcagctttt caaaagttgc cagataaaag tcacgagaca tagtcgcttc atacaagtcc
     7381 tgaccttgat actctaccaa actgccattt tccgcgatga aaataatgtc atcacgaaca
     7441 tcagcaaata atttttccag agacagaaat ccccgacccg aagccaccgc aaagtaaatc
     7501 cctttttcct tgtaggaagc caagagagac ttgagacgat ccatatcaaa gcgtccattc
     7561 ccatctagga aggttccgtc catatccgtt gctactagtt taattgtcat ccttcaatac
     7621 tttctaaatc ttttaactta actgaaacaa tctttgaaac acccgattct tgcatggtca
     7681 ctccatagat ggaatcagcc gctgccatgg ttcccttacg gtgggttaca acgataaact
     7741 ggctgtcctt gtcaaagcgg ttgaggtaat ccccaaaacg tttaacattg gcttcatcca
     7801 gcgcagcttc cacctcatcc aagatgacaa aaggaatggt cttgacacga ataatggaga
     7861 aaagcaaggc aagagccgat agagcttttt caccaccact catgaggtta agcgactgga
     7921 ttttcttacc tggaggttga acagaaatct ccacaccagc tgttaaaagg tcgccctcag
     7981 tcaatatcaa gtctgcctga cctccgccaa acatctgctt gaaggtcact ttaaaggact
     8041 cacgaatagc ttcaaaggtt gatttaaagc gttccttgac ctcatcattc atctctgtaa
     8101 tggtttcaag gagcagattt ttcgctgaca aaatatcatc tcgctgacta tttagaaagt
     8161 ccagacggtt gtgaacttct tcgtactggt caatagcttc caagttgaca ggtcccaatg
     8221 agcgaatagc cttctctaaa tcctgcacct cttgctctgc cagattgagg ttttccaatt
     8281 catgtgcctt ttctagtgct tcagtatagc taatctggta ctggtctgtt aattgatttt
     8341 gtagatggcg caagcgctcg ctgacctttt ctttcttggc ttcagcacgt gtttgcttgc
     8401 gaatccactc ctcattctgc tggcgagctt gatccaagtg actggcaata tcatccagtt
     8461 gaccttctat atcatccaac tcaaactgct tgcgaatcaa accttgttgg agatttgttt
     8521 tctgagtttt ggattcttcc gcctgttgac tgagcaattc tgtatcaacc ttctcaagat
     8581 tgtcaacttt ttcttggagc atgcgctgaa tttcttcttg ttcgatatta agattgtcca
     8641 attccttgcc taaacgctca atatcagcta cttcataacg tttttgccct tgcagttctg
     8701 tcttaagcaa gcgagcttgc gccacctctt cctgcaaatt ttgatagcgt tcttggatgg
     8761 cgtttttatt agacttaatt tcttcaatct cagcttccag attttgcttt tcactggcga
     8821 tagtagcaag gctctcttga catttttcct tatccgcttg ccaatctccc tcagaaagac
     8881 gatctatttc ctcttcttgg agtttccaaa gagtttccag ttcttcaact tgctgactag
     8941 tttgctgata agcgagggac aagccttgct cctgaatacg tgcctgctct ccttgagatt
     9001 tgatggcttc taatctttct gtcaatgcag ccatctggtc ttgcaaggtc ttcaaagccg
     9061 cctcttctga acccaagctt gcttcttctt cagcaatttc tttttgtaat tgctccagtt
     9121 ctggcttgat gaaaatactg ttattctggc gattagctcc acctgcataa gaaccacctg
     9181 tgcgcagctc tgttccgtcc aatgtcacca tacgaacctg ataacgaacc tggcgcgctg
     9241 ctgcacgcgc atgttctaca gtatcaaaga tagccgtcgt agctagcaag ttcttgaaaa
     9301 tggcttccag tctagtatca aaagtcacca actcatctgc catcccaagg aaacctgggc
     9361 ttacagcgat agcatcttgg ttctgactag aaatcgtacg cgccttgata gtggtcaaag
     9421 gaagaaaggt tgcacgaccg gctctgttcc atttaaggaa atcaatcgcc ttggttgccg
     9481 actcttcatc ttctacgatg atatgttggc tactcgctcc aagcgcaatt tctagggcag
     9541 tttgataatg catatcaaag gtcagatgct cactgactgc accaataatc ccacctaggc
     9601 gatctttttc ttggagaaca ctcttaacac ctgcataaaa gttactatga tttctcagga
     9661 tattttccaa actttgagct ctggcctgct tgtttttgag attatccaga cggtcaaaga
     9721 gttggctttg ttgagcttga taggaagttt tctgctcctc ttgctccttg gcaatagctt
     9781 ggtagtcagc caataatttc tgaacctgct ccttggcagt ttcaagctct tccttttgct
     9841 gactagcctt ctctttagct atagctaatt gctctttcag cttttctagt tgatctgctt
     9901 gtttttgaga aagctgacga ctattttcca actcattctc aatacgggtc aactggtttg
     9961 agacatccgc ttcttcttgt aaaagagcta caaagcgttc acgtaagagc tcaatcatct
    10021 gatcaggatc gtctgagaaa gccagcaatt cagcttctaa acgattgagt ttttgattat
    10081 tttggactag atttccctct aacagagcta aagagctttc tttatcagac ttttctttgc
    10141 taagtgaatt tctcttatcc tccaaagcag ccaaacgagc ttgtgcctcc tgttgattca
    10201 aggctacttg ctcggactcc agtttcgata gggctaattt tctttctaaa tcactaatca
    10261 gactagtcaa gtccatcaaa ctgccttggt ctttggccat ttcagcctgt aaatcttggc
    10321 gttgcttttt aagagtttga ttttcttctt ctaatttttc acgcttttgg taataactca
    10381 tcaagagttc ttgaacctga gccaactctt cttctgtcga ctctagttct gccttatttt
    10441 ccttgatttg agcaaccaga acgtctaaat aaatagcctt acgttgtcct tccaagtcta
    10501 aaaacttacg ggcattctca gcttgcttct caagaggctt gatttgatta tccaactcgt
    10561 agataatgtc ctctaagcgg tccagattat cctgagtttg ctgcagttta ctctcggttt
    10621 cttttctgcg agtcttgtat tttaaaactc cagcagcttc ttcaaaaata gctcgtcgtt
    10681 cctcaggctt ggaattaaaa atctcctcaa ccttcccttg ggaaataata gagaaggaat
    10741 ctcgtcccaa tccagtatcc aagaagaggt catgaatatc acgaagacgg actttcttgc
    10801 cgtcaatctt gtattcgcta tctccactac gatagatatg gcgttccacc ctgatttctt
    10861 gacctgcatc cttgataaat ccgtcatgat tatccagagt cacaactaca gaagcataat
    10921 tgagcggttt gcgactttct gttccagcaa agatgacatc cggcatcttg cccccacgga
    10981 gactcttgac actagactcc cccaaagccc aacgcagact ttctgtaata ttggactttc
    11041 cagatccatt gggtccaaca actgccgtca caccttggtc aaaaacgacc ttggtcttat
    11101 cagcaaaaga cttgaacccc tgaatttcga tttcctttaa atacatgaat ccagcccttt
    11161 ctcaacggca tttttggcag cttcctgctc tgctaatttc ttagaacgac cttggccttg
    11221 accgatgctc ttaccttcaa caagaacttc tacatcaaag accttatcgt gagcaggccc
    11281 tgtttcagaa atcacctgat aacgaatagc cacatcacca ttgacctgaa gcaactcttg
    11341 gagatgggtt ttatagtctg taatcatctc aaattcgcct gcttctacct taggaatcat
    11401 aacttggtag ataaattcct tgacctttgc cacatcctta tccaaaagaa gggcaccaag
    11461 aaaggcttca aaggcatcac caagaatggt gtcacgattg cgaccaccag atttttcttc
    11521 ccctttaccc aacttgataa actggtcaaa ctggcaatca cgcgcaaaac cagctaaact
    11581 ctcctcacgg acaatcatag cacggagttt tgataggtca ccttcaggct ttttaggata
    11641 ttttttatat agatattctg aaatcaataa ctgtagaaca gcgtctccta aaaattccaa
    11701 gcgttcattg tgtgaaattt ttaagaggcg gtgctcattg gcataactcg tatgagtaaa
    11761 ggcagtttcc agtaactttt tgtctgcaaa ttcgatttca aaatgattct ttagtacagt
    11821 ttgtaattct ttcataccaa cttctttcta actgataata gtccttttta ttatatcaaa
    11881 aaaagccccc tgagtcactc taaaacggga ctggaaagca tttgggaatt ctttagacag
    11941 agattctcag ttttagcggc aaatttgggt caggataaag aaaaaagccc tattaaaggc
    12001 tttttaggat gtttacatcc accctgaggg aatcgaaccc ccatctcaag aaccggaatc
    12061 ttacgtgata tccattacac taagggtgga aacttgtttt attataacag aaatttgctc
    12121 taataacaag ttttttggtc aaagaccccg tcttagtggg aagcatctcc attccagatg
    12181 gagtttttca cgatcacata atcaacgtgt ttaaggtcag caacctgacg tccacctgca
    12241 taagaaatag cactttgaag gtcttgttcc atctcagtta aagtgtcttg cagatgacct
    12301 ttagcaggaa gcaagatacg tttgccttcc acatttttgt aagcaccttt ttgatattgt
    12361 gaggctgaac cataatattc tttgaactgt tcaccatcga cttcaatcgt tttccctgga
    12421 ctttcaatgt gtcctgcaaa gagggaacca atcatgatca tgctagcacc gaagcggata
    12481 gacttggcaa tatcaccgtg agtacgaatt cctccatcag cgataatcgg tttacgcgca
    12541 gccttggcac accagcgtag agcagccaac tgccaaccac ctgtaccaaa accagtctta
    12601 accttggtga tacaaacctt accaggaccg attccgacct tagtagcatc cgcaccagca
    12661 ttttccaatt cacgcacagc ttctggtgtt cccacatttc cagcaatgac aaaggtatct
    12721 ggcaattctt tcttgatgtg ttgaatcata gaaatcacgc tatccgcatg accatgagca
    12781 atatcaatag tgatatactc aggagcatca gccttgagct ggctaacaaa atcatactca
    12841 tagtccttaa caccgacaga gatagaagca atgagtcctt gatcgtgcat gcgtttaata
    12901 aaaggaatgc gtcctgcctc atcaaaacgg tgcataatgt agaagtaacc acctttagcc
    12961 agttgctctg ctacattttc atccaaaatc gtctgcatat tcgctggcac aacaggtagt
    13021 ttaaaggtgt gatttcctaa agtgacactt gtatccgctt ctgcacggct tttaatgaca
    13081 catttatttg gaatcaattg aatatcttcg taatcaaaaa ttggaaattc atttaacata
    13141 tcgatgtctc gtttcttttg taatgaccta cctatgctct cgcatcacta cgccttttcc
    13201 gacgtttcct taatttatta taaactaaag tacagttttt gtcaaattat tttatgaatt
    13261 gaaatatatt gttcgggttt tgcttgtgtt aaaaataaaa gaaagagggg aaattatttt
    13321 ctccccactt cttcatcaat aatcgcttta aaattttctt ctgaattttc catcatttta
    13381 caaaatgcac tatagaatga ggcattctga gggattcgaa tagaccactt cttcaattga
    13441 gctccataac ctgctaggtt tcccatctca ttatcaataa ccttgtcctt gctcggaaaa
    13501 acaagtccag ccttctcagc ttttaaaata taagaatagg aaatcagctg gaataagtcc
    13561 tcacggttga ttcctttttc agtcaattcc agttttttat attttgcatc tagaacaatc
    13621 tttcgttctc tgtcataaaa atctggatat acttttcgtt tcccaacaga aaatactgaa
    13681 attccatccg tcttatcctt atttctggga tgtacaaaac cttttggcaa caaggtgtaa
    13741 acatactctt cccaaagcca ggcaacatca aagagaatac catagatttt ttgatcttga
    13801 taccctaaac cgtgcttttc ttggtttagg atcatcagac aaagttcttg taagtttctg
    13861 tactcgtgaa agtatgcatg acgtataggt ttagattgat ttctccgagt aatcttagca
    13921 cgatcagcta gtttataaga gggcgttaca cgcacgattt cagatacgtt ttcacgacta
    13981 gttgagagat tatctagtac cccttgacca atgcttttct gattcttaat gtattcaata
    14041 gtgtgacgga ccaactgcat gaggggatta tcataggtga actctctcgt tgtgtaggca
    14101 atatttcccg tgaaaggaag atttttcttg agatggtttc ttacatcaat cactccctta
    14161 acatgactgt cgttatgaga aaatcgatga tattccttat aaagaccttt tcgaatagca
    14221 gcttgtagat acttggggaa gagatacatc aaaagttgat aaagcctctc ttcacgagac
    14281 aaagcaacat ctaaactagt gagattgata tgaagaacct tgtttaagag ataatgcaaa
    14341 aaatggtcat tactctcatc agaaaatcgt gaggaaatcg ttaatctttc ctgaccatat
    14401 ccaagaaaac caatcacatt tcctgtcttg atttcctgat tgatcgtttc caaaatcttt
    14461 tgtttcttat ccaaatctgg agtatgagtc aaatcatttg gaaaaatgaa aatacgttca
    14521 tcttgggata gactttccaa ggttctgtct aaaagaagat tgcttagttt aggatattct
    14581 tcaacaaatt tttctttaat aatcttgtgt tgattatcag taatccgcat catcgttttc
    14641 atcgctttca ttgtcatcag ctactgcctg atctttttgc tcattatttg tcagttcaaa
    14701 tgcttttttc aaagtttcca aagtttcaac ctcatcataa gaacctcgca agtagtcttc
    14761 taggagaggc ttaatataat cagaccagag taattcatag tcaaaatcta catccttcaa
    14821 cttaagaaaa taacttggtc caatatgata atgactgttt aattcctgaa cattttcgat
    14881 agcagcgttc aagtttctga gacgaatttt tgcttcttct gcatggatat tcagctcttt
    14941 atccaacatg ccaacttgac cctcggcagt aacttcaaca aaacgaaaac gacgacgcat
    15001 agcaaaatca aaggtatcca ctgaacgatc aatatcattc atagttccga tgatgtaaac
    15061 attttcgggg atatagaact tttcatcagt ttcgtgtaga tttgcatatt gggtagaaac
    15121 acttcctttt tcaccacgat agccagggtc gatagagaaa aagagttcgc caaaaatctt
    15181 agaaatctcc ccacgattga tttcatcgat gatgaagacg aatttcttat cagtatcaat
    15241 ttctgttggg gaaacatagt ctttcaaacc aaatctcttt ctcaatgttt ctaggacagt
    15301 ttttccacca cttttgtagt attcttgctt attataattt ttatctttat acaactcgta
    15361 aacatatttg ctaggtagtg accatcctgg aacaccacta tcataattta ctgacaaatt
    15421 ttgtctacta ttaacagata agtaagatgt ttttgttata tattcttttt cttcagcaac
    15481 atttatatat tctaagtaag aatcccaagc ctcatcaaaa ttatcttgtc ctccaatcaa
    15541 ttgggtttct tttgctttct gacaaaaatc tttaaaaata ccgtcctgta gcctaaactc
    15601 aatagctcca tccccatttg atactggtct taaaccttct acaaaatccg tataatcata
    15661 tgatgggtga aattgtacaa atccgatttg atcttcgttg ccatccgtta attctttggc
    15721 aatttcttta gcaagataag tctttcctgt gccaggagca ccacgaagga taaggtttgg
    15781 agactttaat agagattgag ttaattgatg acgaaaattg tttgatgaat ccattgtact
    15841 attgtcttcc ttattttcca taaaaagctt agaatactca aaaagcatag ttcctaattt
    15901 cgatggctcc aactcaggat aagttttgga caccagttca attattttat gattttgcag
    15961 ataaggtcca ccttgatttg tctgagaaac aaagtggttc caaaattctt tctgactatt
    16021 aatatttaca ccaaagaaag gatctccctc agaatagata caaagtaatt ttgtcaccat
    16081 agcagaacga ccaataaatt catttgttga tcttttcata tcaaaaatag aattttcaaa
    16141 gttaaaacga ataccttctt tgataatttc atacaaatct gattttaatt ttgaaaatcg
//
LOCUS       DAVLVP010000039        15407 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.039, whole genome shotgun sequence.
ACCESSION   DAVLVP010000039 DAVLVP010000000
VERSION     DAVLVP010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15407
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.039"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            246..593
                     /gene="ssrA"
                     /locus_tag="VYN69_001183"
     tmRNA           246..593
                     /gene="ssrA"
                     /locus_tag="VYN69_001183"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(776..863)
                     /locus_tag="VYN69_001184"
     tRNA            complement(776..863)
                     /locus_tag="VYN69_001184"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(827..829),aa:Ser,seq:gga)
     gene            1025..1765
                     /locus_tag="VYN69_001185"
     CDS             1025..1765
                     /locus_tag="VYN69_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1533461.1"
                     /translation="MLKTERKQLILEELNQHHVVSLEKLVSLLETSESTVRRDLDELE
                     EENKLRRVHGGAELPHSLQEEETIQEKSVKNLQEKKLLAQKAASLIKEKDVIFIDAGT
                     TTAFLIHELVNKNVTVVTNSIHHAAQLVEKQIPTVMVGGNVKTATDASIGGVALNQIN
                     QLHFDRAFIGMNGVDDGYYTTPDMEEGAVKRAILENAKQTYVLVDSSKIGQTCFAKVA
                     PLKRAIVITSQGHELLQVIKEKTEVIEV"
     gene            1762..2673
                     /gene="pfkB"
                     /locus_tag="VYN69_001186"
     CDS             1762..2673
                     /gene="pfkB"
                     /locus_tag="VYN69_001186"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015605104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEV1533462.1"
                     /translation="MIYTVTLNPSIDYIVRLDQVKVGSVNRMDSDDKFAGGKGINVSR
                     VLKRLNIPNTATGFIGGFTGKFITDTLAEEEIETRFVQVAEDTRINVKIKADQETEIN
                     GTGPTVELVQLEELKAILSSLTAEDTVVFAGSSAKNLGNVIYKDLISLTRQTGAQVVC
                     DFEGQTLIDSLDYQPLLVKPNNHELGAIFGVKLESLDEIEKYARELLAKGAQNVIISM
                     AGDGALLVTSEGAYFAKPIKGTVKNSVGAGDSMVAGFTGEFVKSKDAVEAFKWGVACG
                     TATTFSDDLATAEFIKETYGKVEVEKR"
     gene            2670..4622
                     /locus_tag="VYN69_001187"
     CDS             2670..4622
                     /locus_tag="VYN69_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1533463.1"
                     /translation="MKIQDLLRKDVMLLDLQATEKTAVIDEMIKNLTDHGYVTDFETF
                     KEGILAREALTSTGLGDGIAMPHSKNAAVKEATVLFAKSNKGVDYESLDGQATDLFFM
                     IAAPEGANDTHLAALAELSQYLMKDGFADKLRQATSADQVIELFDQASEKTEELVQAP
                     ANDSGDFIVAVTACTTGIAHTYMAQEALQKVAAAMGVGIKVETNGASGVGNQLTAEDI
                     RKAKAIIIAADKAVEMDRFDGKPLINRPVADGIRKTEELINLAISGDAEVYRAANGAK
                     VATASNEKQSLGGAFYKHLMSGVSQMLPFVIGGGIMIALAFLIDGALGVPNENLGNLG
                     SYHELASMFMKIGGAAFGLMLPVFAGYVAYSIAEKPGLVAGFVAGAIAKEGFAFGKIP
                     YAAGGEATSTLAGVSSGFLGALVGGFIAGALVLAIKKYVKVPRSLEGAKSILLLPLLG
                     TILTGFVMLAVNIPMAAINTAMNDFLGGLGGGSAVLLGIVLGGMMAVDMGGPVNKAAY
                     VFGTGTLAATVSSGGSVAMAAVMAGGMVPPLAIFVATLLFKDKFTKEERNSGLTNIIM
                     GLSFITEGAIPFGAADPARAIPSFILGSAVAGGLVGLTGIKLMAPHGGIFVIALTSNA
                     LLYLVSVLGGAIVSGVVYGYLRKPQA"
     gene            4786..7011
                     /locus_tag="VYN69_001188"
     CDS             4786..7011
                     /locus_tag="VYN69_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000908822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEV1533464.1"
                     /translation="MLISLGIAILLIFAAFKLGAAGITLYNLIRLLVGSLAYLAIFGL
                     LIYLFFFKWIRKQEGLLSGFFTIFAGLLLIFEAYLVWKYGLDKSVLKGTMAQVVTDLT
                     GFRTTSFAGGGLIGVALYIPTAFLFSNIGTYFIGSILILVGSLLVSPWSVYDIAEFFS
                     RGFAKWWEGHERRKEERFVKQEEKARQKAEKEARLEQEETEKALLDLPPVDMETGEIL
                     TEEAVQNLPPIPEEKWVEPEIILPQAELKFPEQEDDSDDEDVQVDFSAKEALEYKLPS
                     LQLFAPDKPKDQSKEKKIVRENIKILEATFASFGIKVTVERAEIGPSVTKYEVKPAVG
                     VRVNRISNLSDDLALALAAKDVRIEAPIPGKSLIGIEVPNSDIATVSFRELWEQSQTK
                     AENFLEIPLGKAVNGTARAFDLSKMPHLLVAGSTGSGKSVAVNGIIASILMKARPDQV
                     KFMMVDPKMVELSVYNDIPHLLIPVVTNPRKASKALQKVVDEMENRYELFAKVGVRNI
                     AGFNAKVEEFNSQSEYKQIPLPFIVVIVDELADLMMVASKEVEDAIIRLGQKARAAGI
                     HMILATQRPSVDVISGLIKANVPSRVAFAVSSGTDSRTILDENGAEKLLGRGDMLFKP
                     IDENHPVRLQGSFISDDDVERIVNFIKAQADADYDESFDPGEVSENEGEFSDGDAGGD
                     PLFEEAKSLVIETQKASASMIQRRLSVGFNRATRLMEELEIAGVIGPAEGTKPRKVLQ
                     Q"
     gene            complement(7056..7484)
                     /locus_tag="VYN69_001189"
     CDS             complement(7056..7484)
                     /locus_tag="VYN69_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533465.1"
                     /translation="MSNYRRTSKPKTEHIKKGFTVFQKTVATIASILGLITAGITIMN
                     ALDNNKNIKKEPTTSQTTTIVKEIQKESPKENTSPTKETNTSQEKTQQEETPKSSVKE
                     EKKEDQKTATQDSSTPASSKPATENEKQSNAPTSENKSNQ"
     gene            7622..8764
                     /locus_tag="VYN69_001190"
     CDS             7622..8764
                     /locus_tag="VYN69_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1533466.1"
                     /translation="MIYFDNSATTRPYPEALETYMQVASKILGNPSSLHRLGDQATRI
                     LDASRQQIADLIGKKSDEIFFTSGGTEGDNWIIKGVAFEKAQFGKHIIVSAIEHPAVK
                     ESALWLKAQGFEVDFAPVDNKGFVDVEALEDLIRPDTTLVSIMAVNNEIGSIQPIEAI
                     SEFLADKPTISFHVDAVQALAKIPTEKYLTERVDFATFSGHKFHGIRGVGFIYIKSGK
                     KITPLLTGGGQERDYRSTTENVAGIAATAKALRLSMEKLDIFRSKTGQMKAVIRQALL
                     DYPDIFVFSDEEDFAPHILTFGIKGVRGEVIVHAFEDYDIFISTTSACSSKAGKPAGT
                     LIAMGVDKDKAKSAVRLSLDLENDMSQVEQFLTKLKLIYNQTRKVR"
     gene            8773..9987
                     /gene="thiI"
                     /locus_tag="VYN69_001191"
     CDS             8773..9987
                     /gene="thiI"
                     /locus_tag="VYN69_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001200101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEV1533467.1"
                     /translation="MQYSEIMIRYGELSTKGKNRMRFINKLRNNISDVLSIYTQVKVT
                     ADRDRAHAYLNGADYTAVAESLKQVFGIQNFSPVYKVEKSVEVLKSSVQEIMRDIYKE
                     GMTFKISSKRSDHNFELDSRELNQTLGGAVFEAIPNVQVQMKSPDINLQVEIREEAAY
                     LSYETIRGAGGLPVGTSGKGMLMLSGGIDSPVAGYLALKRGVDIEAVHFASPPYTSPG
                     ALKKAQDLTRKLTKFGGNIQFIEVPFTEIQEEIKAKAPEAYLMTLTRRFMMRITDRIR
                     EVRNGLVIINGESLGQVASQTLESMKAINAVTNTPIIRPVVTMDKLEIIDIAQEIDTF
                     DISIQPFEDCCTIFAPDRPKTNPKIKNAEQYEARMDVEGLVERAVAGIMITEITPQAE
                     KDEVDDLIDNLL"
     gene            10122..10946
                     /locus_tag="VYN69_001192"
     CDS             10122..10946
                     /locus_tag="VYN69_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001076725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEV1533468.1"
                     /translation="MNQSYFYLKMKEHKLKVPYTGKERRVRILLPKDYEKDTDRSYPV
                     VYFHDGQNVFNSKESFIGHSWKIIPAIKRNPDISRMIVVAIDNDGMGRMNEYAAWKFQ
                     ESPIPGQQFGGKGVEYAEFVMEVVKPFIDETYRTKADCQHTAMIGSSLGGNITQFIGL
                     EYQDQIGCLGVFSSANWLHQEAFNRYFECQKLSPDQRILIYVGTEEADDTDKTLMDGN
                     IKQAYIDSSLCYYHDLIAGGVHLDNLVLKVQSGAIHSEIPWSENLPDCLRFFAEKW"
     gene            10968..11707
                     /locus_tag="VYN69_001193"
                     /pseudo
     CDS             10968..11707
                     /locus_tag="VYN69_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546805.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
     gene            11720..12886
                     /locus_tag="VYN69_001194"
     CDS             11720..12886
                     /locus_tag="VYN69_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001108652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1533469.1"
                     /translation="MNYLVISPYYPQNFQQFTIELANKGITVLGIGQESYEQLDEPLR
                     NSLTEYFRVDNLENIDEVKRAVAFLFYKHGPIDRIESHNEYWLELDATLREQFNVFGA
                     KPEDLKKTKYKSEMKKLFKKAGVPVVPGAVIKTEADVDQAVKEIGLPMIAKPDNGVGA
                     AATFKLETEDDINHFKQEWDHSTLYFFEKFVTSSEICTFDGLVNKDGKIVFSTTFDYA
                     YTPLDLMIYKMDNSYYVLKDMDPKLRKYGEAIVKEFGMKERFFHIEFFREGDDYITIE
                     YNNRPAGGFTIDVYNFAHSLDLYRGYAAIVAGEEFPASDFETQYCLATSRRANAHYVY
                     SEEDLLAKYSQQFKVKKVMPAAFAELQGDYLYMLTTPSRQEMDQMIADFGQRQE"
     gene            13394..14887
                     /locus_tag="VYN69_001195"
     CDS             13394..14887
                     /locus_tag="VYN69_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1533470.1"
                     /translation="MITGELKNKIDQLWEILWTEGNANPLTNIEQLTYLLFMKDLDSV
                     ELGRESDAEFLGIPYEGVFPKDKPEYRWSTFKNIGDAQEVYRLMTQEIFPFIKNLKGD
                     TDDTAFSRYMREAIFQINKPATLQKAISILDVFPTRGLDVDFDNDKQSITDIGDIYEY
                     LLSKLSTAGKNGQFRTPRHIIDMMVELMQPTIKDIISDPAMGSAGFLVSASRYLKRKK
                     DEWETNTDNINHFHNQMFHGNDTDTTMLRLGAMNMMLHGVENPQISYLDSLSQDNEEA
                     DKYTLVLANPPFKGSLDYNSTSNDLLATVKTKKTELLFLSLFLRTLKPGGRAAVIVPD
                     GVLFGSSKAHKGIRQEIVENHKLDAVISMPSGVFKPYAGVSTAILIFTKTGNGGTDKV
                     WFYDMKADGLSLDDKRQPISDNDIPDIIERFHHLEKEAERQRTDQSFFVPVAEIKEND
                     YDLSINKYKEIEYEKVEYEPTEVILKKINDLEKEIQAGLAELEKLLK"
     gene            14900..>15407
                     /locus_tag="VYN69_001196"
     CDS             14900..>15407
                     /locus_tag="VYN69_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1533471.1"
                     /translation="MKKVKLGEVCEILSGYAFKSSQFNDNKIGLPLIRIRDVERGFSD
                     TYFEGTYPEEYLIKNGDLLITMDGSFILKKWEGDLALLNQRVCKIKITDKSVDEGYIS
                     WLIPKFLKEIEDKTPFVTVKHLSVAKIKDISFVLPNKLEQKLIAKKLNTISQIYDFRK
                     IQSEKFNEL"
BASE COUNT         4582 a         2857 c         3432 g         4536 t
ORIGIN      
        1 aaaattctca tctgaaagta ttttagaata attctcttcg tgaatttctt caaaacagat
       61 agcttcatct taggtatgtg ttaattggat ttcgtaagct ctatttacaa tcgtaaagtt
      121 atgcactgag aaagttgtag ttagtcttcc agtttgctaa atttctcgaa tagtatcttt
      181 tttaggtatg tgatttcttt ttgcattttt gagttagata aggtataatg gttttattgt
      241 cttttggggt cgttacggat tcgacaggca ttatgaggca tattttgcga ctcgtgtggc
      301 gacgtaaacg ctcagttaaa tataactgca aaaaataaca cttcttacgc tctagctgcc
      361 taaaaaccag caggcgtgac ccgatttgga ttgctcgtgt tcaatgacag gtcttattat
      421 tagcgagata cgattaagcc ttgtctagcg gcttgataag agattgatag actcgcagtt
      481 tctaggcttg agttatgtgt cgaggggttg ttaaaataat acataaccta tggttgtaga
      541 caaatatgtt ggcaggtgtt tggacgtggg ttcgactccc accggctcca ttattccttt
      601 gcattctttt gcattccttg gtaaaacgtt gttaaatcaa cgttttttat ttttatcttt
      661 ggtattcctt tgcattcttt tgctaaaaag ggagtcacaa acagacccta ttttaaaaaa
      721 aggatagaaa aaaggataca acatttgtcg catcctagaa ataatctttt ttcgacggaa
      781 gacatgggat tcgaacccac gcacgctatt acacgcctac cgcgtttcca acacggcctc
      841 ttaagcctct tgagtaatct tccaatactt actcaaatag tctaccataa aggctcttat
      901 cttgcaataa aaattctaga aataagaaaa atgatagatt ttgaaagaaa atgacaaaaa
      961 atgcttgact tcgaaataaa gtatggtaga atgaatagtg taaacgataa caggaggtga
     1021 ttcagtgtta aaaacagaac gtaaacaact gattttagag gagttaaatc aacatcatgt
     1081 agtttctcta gaaaaattag ttagtttgct agaaacgtca gaatcaacgg ttcgaagaga
     1141 tttggatgag ttggaagagg aaaacaagct tcgtcgtgtg catggtggag cagaactccc
     1201 ccactcctta caggaagaag aaaccattca agaaaaatct gtcaaaaacc ttcaagaaaa
     1261 gaagttgctg gctcagaaag cagcctctct cattaaagaa aaagatgtca tctttatcga
     1321 tgctggaaca acaactgctt ttttgattca tgaattggtc aataagaatg ttacagttgt
     1381 gaccaactcc attcaccatg ccgctcagtt ggttgaaaag cagattccaa ctgtcatggt
     1441 tggaggaaac gtcaagacgg cgacagatgc tagtatcggg ggcgttgctc ttaaccagat
     1501 taaccaattg cactttgacc gtgcctttat cggaatgaat ggtgttgacg atggctatta
     1561 tacgactcct gatatggagg agggagctgt gaaaagagct attttggaga atgccaagca
     1621 gacctacgtc ttggtggatt cgtcaaaaat tggacaaact tgctttgcca aggtagcacc
     1681 actcaaacgc gctatcgtta tcactagtca agggcatgag ctcttgcagg ttattaagga
     1741 gaaaacggag gtaatagaag tatgatttat acagtcacac tcaatccatc cattgactat
     1801 atcgttcgtt tggaccaagt caaagttggt agtgtcaatc gtatggacag tgatgataag
     1861 tttgctggtg ggaaaggaat caatgtcagc cgtgtcttga aacgtttgaa tataccaaat
     1921 acagcgacgg gatttatcgg tggctttact ggtaaattta tcacagatac tttagcagag
     1981 gaagaaatcg agacacgttt tgtccaggtg gcagaagata ctcgtatcaa tgttaaaatt
     2041 aaagcagacc aagaaacaga aatcaacgga acgggtccaa ccgttgaact ggttcagcta
     2101 gaagaattga aagctatttt atctagtctg acagcagaag atacagttgt ctttgcaggt
     2161 tcaagtgcta aaaatctagg caatgttatc tataaggatt tgatttcctt gacgcgccag
     2221 actggtgcgc aagtggtctg tgactttgaa ggacagacct taattgatag tttggactac
     2281 cagcctcttc ttgtaaaacc aaacaatcat gaacttggag cgatttttgg ggttaaactc
     2341 gaaagtttag atgaaattga gaaatacgct cgtgagttac tggctaaggg tgctcaaaat
     2401 gttattatct ctatggctgg tgatggtgcc cttcttgtca catctgaggg agcttacttt
     2461 gctaaaccaa tcaaaggaac agtcaaaaat tcagttgggg ctggtgattc tatggttgct
     2521 ggattcacag gtgaatttgt caaatcaaaa gacgcagtag aagccttcaa atggggagtg
     2581 gcttgcggaa cggcaactac cttctcagat gacttggcaa cggcggaatt tattaaagaa
     2641 acatatggaa aagttgaggt agaaaaacga tgaaaattca agacctattg agaaaagatg
     2701 tcatgttgct ggatttgcag gcaactgaaa aaacagctgt catcgacgag atgattaaaa
     2761 atttgacaga ccacggttat gtaacagatt ttgaaacatt taaagaagga attttggcgc
     2821 gtgaagcttt gacttctact ggtttgggtg atggaatcgc aatgcctcac agcaaaaacg
     2881 ctgctgtcaa agaagcgaca gttctatttg ctaagtcaaa taagggtgtt gactacgaga
     2941 gcttggatgg acaagcaact gacctcttct tcatgattgc agctccagaa ggtgccaatg
     3001 atactcactt ggcagccttg gcagaattgt ctcaatactt gatgaaagac ggttttgcag
     3061 acaaacttcg tcaagcaaca tctgcagacc aagttatcga actttttgac caagcttcag
     3121 aaaaaactga ggaacttgtt caagcacctg ctaatgactc tggtgacttt atagtagctg
     3181 ttacagcttg tacaacaggt attgcccaca cttacatggc ccaagaagcc cttcaaaaag
     3241 tagctgctgc aatgggggtt ggtatcaagg tcgaaaccaa cggtgctagc ggtgttggaa
     3301 atcaactaac tgcagaagat atccgtaagg ctaaagctat tatcattgca gcagacaagg
     3361 ccgttgaaat ggatcgtttc gatggcaaac ctttgatcaa tcgtccagtt gctgacggta
     3421 tccgtaagac agaagaattg atcaacttgg ctatttcagg agatgcggaa gtctaccgtg
     3481 ccgctaatgg tgccaaagtc gcaacagcct ctaacgaaaa acaaagcctt ggtggtgcct
     3541 tctacaaaca cttgatgagt ggtgtatctc aaatgttacc attcgttatc ggtggtggta
     3601 tcatgattgc ccttgccttc ttgattgacg gtgctttggg tgttccaaat gaaaaccttg
     3661 gcaatcttgg ttcttaccat gagttagctt ctatgttcat gaaaattggt ggagctgcct
     3721 ttggtttgat gcttccagtc tttgcgggtt atgttgccta ctctattgct gaaaaaccgg
     3781 gtttggtagc aggtttcgtg gctggtgcta ttgccaaaga aggttttgcc tttggtaaaa
     3841 ttccttatgc cgcaggtggt gaagcaactt caactcttgc aggtgtctca tctggtttcc
     3901 taggtgccct tgttggtgga tttatcgcag gtgccttggt tcttgccatc aagaaatacg
     3961 ttaaagttcc tcgttcactc gaaggtgcta aatcaatcct tctattgcca cttcttggaa
     4021 caatcttgac aggatttgtt atgctagctg tgaatatccc aatggctgca atcaacactg
     4081 ctatgaatga cttcctaggc ggtcttggag gaggttcagc tgtccttctt ggtatcgtcc
     4141 ttggtggaat gatggctgtt gacatgggtg gaccagttaa taaagcagct tatgtctttg
     4201 gtacaggtac gcttgcagca actgtttctt caggtggttc tgtagccatg gcagcagtta
     4261 tggctggagg aatggtgcca ccacttgcaa tctttgtcgc aactcttctt ttcaaagata
     4321 aatttactaa ggaagaacgt aactctggtt tgacaaacat catcatgggc ttgtcattta
     4381 tcactgaggg agcgattcca tttggtgccg ctgacccagc tcgtgcgatt ccaagcttca
     4441 tccttggttc agcagtagca ggtggactcg ttggtcttac tggtatcaaa ctcatggcgc
     4501 cacacggagg aatcttcgtt atcgccctta cttcaaatgc tctcctttac ctcgtttctg
     4561 tcttgggagg agcaatcgta agtggtgtgg tttatggtta cctacgcaaa ccacaagcat
     4621 aaaaaataga aaaatgaaaa gattggaccg tttggtgcag tctttttctc ttcccgaaat
     4681 gcctgtgaaa tatggtataa tagaagaatg gcaaacaaga atacaagtac aacaagacgg
     4741 agaccgtcta aagcagaact ggaaagaaaa gaagcgattc aacgaatgtt gatttcgtta
     4801 ggaattgcga ttttattgat tttcgcagcc ttcaaattag gggctgcagg tataaccctt
     4861 tataatttaa ttcgcttgct agtgggtagc ctagcttatc tggcgatatt cggcctatta
     4921 atctatctct tctttttcaa gtggatacga aaacaggaag gactcttatc tggctttttc
     4981 accatatttg ctggcttact cttgattttt gaggcctact tggtttggaa atatggtttg
     5041 gacaagtccg ttctaaaagg gaccatggct caggttgtga cagatctgac tggttttcga
     5101 acgactagct ttgctggagg gggcttgatt ggggtcgctc tttatattcc aacagccttt
     5161 ctcttttcaa atatcggaac ttactttatt ggttctatct tgattttagt gggttctctc
     5221 ctagtcagcc cttggtctgt ttacgatatt gctgaatttt tcagtagagg ctttgccaaa
     5281 tggtgggaag ggcacgagcg tcgaaaagag gaacgctttg tcaaacaaga agaaaaagct
     5341 cgccaaaagg ctgagaaaga ggctagatta gaacaagaag agactgaaaa agccttactc
     5401 gatttgcctc ctgttgatat ggaaacgggt gaaattctga cagaggaagc tgttcaaaat
     5461 cttccaccta ttccagaaga aaagtgggtg gaaccagaaa tcatcctgcc tcaagctgaa
     5521 cttaaattcc ctgaacagga agatgactca gatgacgaag atgttcaggt cgatttttca
     5581 gccaaagaag cccttgaata caaacttcca agcttacaac tctttgcacc agataaacca
     5641 aaagatcagt ctaaagagaa gaaaattgtc agagaaaata tcaaaatctt agaagcaacc
     5701 tttgctagct ttggtattaa ggtaacagtt gaacgggccg aaattgggcc atcagtgacc
     5761 aagtatgaag tcaagccggc tgttggtgta agggtcaacc gcatttccaa tctatcagat
     5821 gacctcgctc tagccttggc tgccaaagat gtccggattg aagcaccaat ccctgggaaa
     5881 tccctaatcg gaattgaagt gcccaactcc gatattgcca ctgtatcttt ccgagaacta
     5941 tgggaacaat cgcaaacgaa agcagaaaat ttcttggaaa ttcctttagg gaaggctgtt
     6001 aatggaaccg caagagcttt tgacctttct aaaatgcccc acttgctagt tgcaggttca
     6061 acgggttcag ggaagtcagt agcagttaac ggcattattg ctagcattct catgaaggcg
     6121 agaccagatc aagttaaatt tatgatggtc gatcccaaga tggttgagtt atctgtttac
     6181 aatgatattc cccacctctt gattccagtc gtgaccaatc cacgtaaagc cagcaaggct
     6241 ctgcaaaagg ttgtggatga aatggaaaac cgttatgaac tctttgccaa ggtgggagtt
     6301 cggaatattg caggttttaa tgccaaggta gaagagttca attcccagtc tgagtacaag
     6361 caaattccgc taccattcat tgtcgtgatt gtggatgagt tggctgacct catgatggtg
     6421 gccagcaagg aagtggaaga tgctatcatc cgtcttgggc agaaggcgcg tgctgcaggt
     6481 atccacatga ttcttgcaac tcagcgtcca tctgttgatg tcatctctgg tttgattaag
     6541 gccaatgttc catctcgtgt agcatttgcg gtttcatcag gaacagactc ccgtacgatt
     6601 ttggatgaaa atggagcaga aaaacttctt ggtcgaggag acatgctctt taaaccgatt
     6661 gatgaaaatc atccagttcg tctccaaggc tcctttatct cggatgacga tgttgagcgc
     6721 attgtgaact tcatcaaggc tcaggcagat gcagactacg atgagagttt tgatccaggt
     6781 gaggtttctg aaaatgaagg agaattttcg gatggagatg ctggtggtga tccgcttttt
     6841 gaagaagcta agtctttggt tatcgaaaca cagaaagcca gtgcgtctat gattcagcgt
     6901 cgtttatcag ttggatttaa ccgtgcgacc cgtctcatgg aagaactgga gatagcaggt
     6961 gtcatcggtc cagctgaagg taccaaacct cgaaaagtgt tacaacaata aaaaaatagc
     7021 ttctttccga gtttggaggg aagctatttt agtggctatt gattgctttt attttctgaa
     7081 gttggcgcat tggactgttt ttcgttttca gtagcaggtt tacttgaagc aggagtagaa
     7141 gagtcctgag ttgctgtttt ctgatcttct tttttctctt ccttgacgct agattttggt
     7201 gtttcctctt gctgtgtttt ttcttgacta gtgttagtct ctttagttgg actggtgttt
     7261 tccttagggg attccttttg gatttctttg acaatggttg tcgtctggct tgtcgtaggt
     7321 tcttttttaa tatttttgtt attatccaag gcgttcatga tcgtgatacc cgcggtaatc
     7381 aagccaagga tactagcgat tgtagcaacg gttttttgaa agactgtaaa gccttttttg
     7441 atgtgttctg ttttcggttt tgaagttcta cgatagttag acatacattc tctcctttga
     7501 ttatgattta ttatactctg tttctttaaa aaaatcttaa aaaaagagaa attgcccttt
     7561 cttgtcgcag gctccgtttc atgatacaat agaaggagtg attttagaaa gcgtgagaaa
     7621 catgatttac tttgataatt cggcgacgac caggccctat ccagaagccc ttgaaaccta
     7681 tatgcaggtg gcttcaaaaa ttttaggaaa tccatctagt ctccatcgtt tgggagacca
     7741 ggcaacacga attttagatg cttcccgtca acagattgca gatttaatcg gtaagaaaag
     7801 tgatgaaatc ttctttacat ctggtgggac agaaggggat aactggatca tcaagggagt
     7861 agcatttgaa aaagcccagt ttggcaagca catcatcgta tcagccattg aacatccagc
     7921 agttaaggaa tcagccctct ggttgaaagc tcaaggtttt gaagtggatt ttgctccagt
     7981 tgataacaaa ggatttgtgg atgttgaggc gctagaagat ttgataagac ctgatacgac
     8041 cctcgtttcc atcatggctg tgaacaatga aatcggctct atccaaccta ttgaggctat
     8101 ttcagaattc ttggcagaca agccaactat ttccttccac gttgatgcgg ttcaggcgct
     8161 tgccaagatt ccgactgaaa agtatctgac agaacgagtg gatttcgcga ctttctctgg
     8221 tcacaagttc cacggaatcc gtggtgttgg ttttatctat atcaagtctg gcaagaagat
     8281 tacgcctctt ttaacaggtg gtggtcagga acgtgattac cgttcgacaa ctgaaaatgt
     8341 ggcaggaatt gcagcgaccg ctaaggctct ccgtttatct atggaaaagc tagatatctt
     8401 taggagcaag actgggcaga tgaaggcagt gattcgtcaa gcacttctgg actatccgga
     8461 tatatttgtc ttttcagatg aggaagactt cgcccctcat attctgactt ttggaatcaa
     8521 aggtgttcga ggtgaagtca tcgttcacgc ctttgaagac tatgatattt tcatctcaac
     8581 aacctcagct tgttcatcta aggcaggaaa accagccggt accttgattg ccatgggagt
     8641 ggacaaagat aaggccaagt cagctgtgcg tcttagccta gacttggaaa atgatatgag
     8701 tcaggtcgag cagtttttga ccaagttaaa attgatttac aatcaaacta gaaaagtaag
     8761 ataggagcat tcatgcagta ttcagaaatt atgattcgct acggagagtt gtcaaccaag
     8821 ggtaaaaacc gtatgcgttt catcaataaa cttcgtaata atatttcgga cgttttgtct
     8881 atctataccc aagttaaggt aacagcagat cgcgaccgtg cccacgctta cctcaatgga
     8941 gctgattaca cagcagttgc agaatctctc aaacaagttt ttggaattca aaacttttct
     9001 cctgtttata aggttgaaaa atctgtagaa gttttgaagt cttctgtcca agagattatg
     9061 cgggacatct acaaggaagg tatgaccttt aagatttcta gcaagcgtag cgaccacaac
     9121 tttgaacttg atagtcgtga actcaaccaa acacttggag gggctgtatt cgaagccatt
     9181 ccaaatgtgc aagttcaaat gaaaagtcct gacatcaatc ttcaggtgga gattcgtgaa
     9241 gaagcagcct atctttctta tgaaaccatt cgtggggctg gtggtttgcc agttggaact
     9301 tcaggtaaag ggatgctcat gttgtcagga gggattgact cacctgtagc aggttatctt
     9361 gctcttaagc gtggggtgga tatcgaggca gttcactttg ctagtccacc atatactagt
     9421 cctggtgccc tcaagaaagc gcaggacttg acccgtaaat tgaccaagtt tggcggaaat
     9481 atccagttta tcgaggtgcc tttcacagag attcaagagg aaatcaaagc caaagcgcca
     9541 gaagcttatt tgatgactct aactcgtcgc tttatgatgc ggattactga ccgtattcgt
     9601 gaggtacgaa atggtttggt tatcatcaat ggggaaagtc taggtcaagt agccagccaa
     9661 acccttgaaa gtatgaaggc tatcaatgct gttaccaaca ctcccatcat tcgtcctgtg
     9721 gttaccatgg acaagttgga aatcattgac atcgcccagg aaatcgatac ctttgacatt
     9781 tcaatccaac cgtttgaaga ctgttgtacc atttttgcac cagatcgtcc aaaaacaaat
     9841 cctaaaatta agaatgcgga gcagtacgaa gcgcgtatgg atgttgaagg cttggttgag
     9901 cgagcagtgg ctggaatcat gattactgaa atcacacctc aagccgaaaa agatgaagtt
     9961 gatgacttga ttgacaatct gctctaattc agaaaatcca aaagaatagc gaaaatcagt
    10021 aaaaaaagtt agttttttct ctaaaaacag gtaaaaaact aacttttttt atttttatga
    10081 tataatgata taaaattttg aatatagaga gttttctgac aatgaatcaa tcctactttt
    10141 atctaaaaat gaaagaacac aaactcaagg ttccttatac aggtaaggag cgccgtgtac
    10201 gtattcttct tcctaaagat tatgagaaag atacagaccg ttcctatcct gttgtatact
    10261 ttcatgacgg gcaaaatgtt tttaatagca aagagtcttt cattggacat tcatggaaga
    10321 ttatcccagc tatcaaacga aatccggata tcagtcgcat gattgtcgtt gctattgaca
    10381 atgatggtat ggggcggatg aatgagtatg cggcttggaa gttccaagaa tctcctatcc
    10441 cagggcagca gtttggtggt aagggtgtgg agtatgctga gtttgtcatg gaggtggtca
    10501 agccttttat cgatgagacc tatcgtacaa aagcagactg ccaacatacg gctatgattg
    10561 gttcctcact aggaggcaat attacccagt ttatcggttt ggaataccaa gaccaaattg
    10621 gttgcttggg cgttttttca tctgcaaact ggctccacca agaagccttt aaccgctatt
    10681 tcgagtgcca gaaactatcg cctgaccagc gcatcttaat ctatgtagga acagaagaag
    10741 cagatgatac agacaagacc ttgatggatg gcaatatcaa acaagcctat atcgactcgt
    10801 cgctttgcta ttaccatgat ttgatagcag ggggagtaca tctggataat cttgtgctaa
    10861 aagttcagtc tggtgccatc catagtgaaa tcccttggtc agaaaatcta ccagattgtc
    10921 tgagattttt tgcagaaaaa tggtaagtta agaaaggaaa aaacgaaatg catattgaac
    10981 atcttagcca ctggagtggt catcttaacc gtgaaatgta ccttaaccgt tatggacatg
    11041 gtgggattcc agttgtggtc tttgcttcat caggtggtag tcacaacgaa tactatgatt
    11101 ttggcatgat tgatgcctgt gcttccttta tcgaggaagg ccttgtccag ttctttaccc
    11161 tatctagttt ggatagtgag agctggttgg ctacttggaa aaatgctcat gaccaagcgg
    11221 aaatgcaccg tgcctacgaa cgttatgtga ttgaggaggc cattctttta tcaagcacaa
    11281 gacaggttgg tttgatggca tgatgacgac aggttgctct atgggagcct atcatgcact
    11341 caatttcttc ctccagcatc cagatgtctt taccaaagtg attgctctca gtggtgttta
    11401 cgacgcacgt ttctttgtcg gtgattacta caacgatgat gctatttacc aaaactcgcc
    11461 agtagattat atttggaacc aaaacgacgg ctggtttatt gaccgttacc gtcaggcaga
    11521 gattgtgctg tgtacggggc ttggagcctg ggaacaagat ggtttgccat ccttttacaa
    11581 gctcaaagaa gcctttgaca agaaacaaat tccagcctgg tttgctgaat ggggacatga
    11641 tgtcgcccat gactgggaat ggtggcgtaa acaaatgcct tatttcctcg gtaatctcta
    11701 tttataaaag gagttaccta tgaattacct tgttatttct ccctactatc cacaaaactt
    11761 tcaacagttt accatcgaac tagctaataa aggcatcaca gtcttgggaa ttggtcaaga
    11821 gtcttacgag caattggatg agcccttgcg caatagcttg accgagtatt ttcgtgttga
    11881 taatcttgag aacatagatg aagtcaaacg tgcagtggct tttctctttt ataaacatgg
    11941 tccaattgac cgcatcgagt ctcacaatga atactggctt gagctagacg caacactcag
    12001 agaacaattc aatgtttttg gtgccaaacc agaggatctc aaaaagacga aatataagtc
    12061 tgaaatgaag aaacttttca aaaaagcagg tgttcctgtg gtacctggag ctgttatcaa
    12121 gacggaagca gatgttgatc aagcagtgaa agaaatcggt cttccaatga ttgccaaacc
    12181 tgataatgga gtgggagcag ccgcaacctt taaacttgag acagaagacg atatcaatca
    12241 cttcaagcaa gaatgggacc attcaaccct ttatttcttt gaaaaatttg tcacttccag
    12301 cgaaatctgt acctttgacg ggctcgtgaa caaggatgga aagattgttt tctcaacaac
    12361 ctttgactac gcctatacac cgcttgacct catgatttat aagatggaca attcttatta
    12421 tgtgctcaag gatatggatc ctaaactgcg caagtatggg gaagcaattg tcaaagaatt
    12481 tggtatgaaa gaacggtttt tccatattga gttcttccgt gagggggacg attatattac
    12541 catcgagtac aataaccgcc ctgcaggtgg ttttaccatt gatgtttata actttgctca
    12601 ttccttggac ctttatcgtg gctatgcagc tattgtcgca ggagaggagt tcccggcgtc
    12661 agactttgaa actcagtatt gtttggctac ttctcgccgt gcaaatgctc actatgttta
    12721 ttcagaagag gatttgcttg ccaaatatag ccagcagttc aaggttaaaa aagtcatgcc
    12781 agctgccttc gcggaacttc aaggagatta cctgtatatg ctgacaactc cgagtcgaca
    12841 agaaatggat cagatgattg ctgatttcgg acaacgtcaa gaataagaac tatcggatta
    12901 aggaaattca ctcccttaat ccttttgttt tgtctggtaa aaaataagag catcctaaca
    12961 aggtagctat cataaaactt gttcgataac tatttgaagc aggattaggt ggctagaaat
    13021 tgaattttaa catttcaatt tagtcatagt attgcgtttt cgtatcctta aatcagctaa
    13081 aaggatccat gacgacacct atacgatata gttttcagga taccaaacca gtctattaat
    13141 actcaatgaa aatcaaagag caaactagga agctagccgc aggttgctca aaacactgtt
    13201 ttgaggttgt ggatagaact gacagagtca gtatcatata cctacggcaa ggtgaagctg
    13261 acgtggtttg aagagatttt cgaagagtat aaaatattca ggtgacgcat agatatagtt
    13321 aattgaagct ttgtttgaaa tctgataaaa taatgatatt actaagtttt aaaaactaaa
    13381 gaaaagggaa gatatgatta caggcgaatt aaaaaataaa atcgatcagc tgtgggaaat
    13441 tctttggaca gaaggaaacg caaatccttt aacaaatatt gaacagttga cttatctctt
    13501 atttatgaaa gatttggata gtgtcgagct tggacgtgaa agtgatgctg aatttctagg
    13561 gattccttat gagggagttt ttccaaaaga taaacctgaa taccgttggt caacttttaa
    13621 aaatatagga gatgctcagg aagtttatcg tttaatgact caggagattt ttccgtttat
    13681 taaaaatctc aagggggata cagatgatac agccttttca cgatatatgc gagaagctat
    13741 ttttcaaata aataaacctg ctacgcttca aaaggcaatt tctatcttag atgtttttcc
    13801 aactagggga ttagatgtag attttgataa tgacaaacaa agtattactg atatcggaga
    13861 tatctatgaa tatctgttat caaaattgtc gaccgcaggt aaaaatggac agttccgtac
    13921 acctcgtcac atcatcgata tgatggttga gttgatgcaa ccgactatca aagatatcat
    13981 ctcagatccc gctatgggtt ctgctggctt cttagtatct gctagccgtt acttaaagcg
    14041 taagaaagat gaatgggaaa ccaatacaga taatatcaat cattttcata atcagatgtt
    14101 tcatggaaat gatacggata cgactatgtt gagacttggg gcgatgaaca tgatgctaca
    14161 tggagtagaa aatccacaaa tcagttacct tgactcgctg tctcaagata atgaagaagc
    14221 cgataaatat actttggttt tagcaaatcc tccttttaag ggctcacttg actacaattc
    14281 aacctctaat gaccttcttg caaccgtaaa aaccaaaaaa acagaattac tctttctttc
    14341 tcttttcttg cgaactttaa aaccaggtgg acgagcagca gttatcgtac ctgatggtgt
    14401 cctttttggt tcgtctaaag ctcataaagg aattcgtcag gaaattgtag agaatcataa
    14461 gcttgatgct gtaatctcaa tgcctagtgg tgtgttcaag ccttatgctg gagtttcaac
    14521 tgccattctc atctttacaa aaactggtaa tggtggtact gacaaagtct ggttttacga
    14581 tatgaaagcg gatggtttaa gtttggatga taagcgacaa ccgattagcg acaatgatat
    14641 tccagatatt atcgaacgct ttcatcatct tgaaaaagaa gcagaacgtc agagaacgga
    14701 tcaatctttc tttgttccag ttgctgagat aaaggaaaat gattatgatt tgtctatcaa
    14761 taaatataaa gagattgagt atgaaaaagt tgagtatgaa ccaacagaag tcatattaaa
    14821 gaaaatcaat gatttagaaa aagaaattca agctggcttg gctgaattgg aaaaattact
    14881 caagtaggga ggtggctgta tgaaaaaagt gaagttgggg gaagtttgtg agattttatc
    14941 tggttacgct tttaaatcat cacaatttaa tgataacaaa attggtctcc ccttaatacg
    15001 aattcgagat gtagaaagag gtttttctga tacctacttt gaaggaactt atcccgaaga
    15061 atatcttatt aaaaatggag acttattgat tacaatggat ggcagtttta tattgaaaaa
    15121 atgggagggg gatttagcgc tacttaatca acgtgtctgt aaaattaaaa ttacggataa
    15181 aagtgtagat gaaggttata tatcttggtt gattcctaaa tttttaaaag aaattgaaga
    15241 taagacccct tttgttacag tgaaacatct ttcagtagct aaaataaaag atatctcatt
    15301 tgtattgcct aataaactag aacaaaagct aattgcaaaa aaactaaata cgataagtca
    15361 aatttatgat tttcgtaaaa ttcaatcaga aaaatttaac gagctcg
//
LOCUS       DAVLVP010000040        15264 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.040, whole genome shotgun sequence.
ACCESSION   DAVLVP010000040 DAVLVP010000000
VERSION     DAVLVP010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 15264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15264
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.040"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(102..2333)
                     /locus_tag="VYN69_001197"
     CDS             complement(102..2333)
                     /locus_tag="VYN69_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEV1533472.1"
                     /translation="MLCQNCKINDSTIHLYTNLNGKQKQIDLCQNCYKIIKTDPNNSL
                     FKGMTDLNNRDFDPFGDFFNDLNNFRPSSNTPPIPPTQSGGGYGGNGGYGSQNRGSAQ
                     TPPPSQEKGLLEEFGINVTEIARRGDIDPVIGRDDEIIRVIEILNRRTKNNPVLIGEP
                     GVGKTAVVEGLAQKIVDGDVPHKLQGKQVIRLDVVSLVQGTGIRGQFEERMQKLMEEI
                     RKREDIILFIDEIHEIVGAGSASDGNMDAGNILKPALARGELQLVGATTLNEYRIIEK
                     DAALERRMQPVKVDEPTVDETITILKGIQKKYEDYHHVQYTDAAIEAAATLSNRYIQD
                     RFLPDKAIDLLDEAGSKMNLTLNFVDPKVIDQRLIEAENLKSQATREEDFEKAAYFRD
                     QIAKYKEMQKKKITDQDTPIISEKTIEHIIEQKTNIPVGDLKEKEQSQLIHLAEDLKS
                     HVIGQDDAVDKIAKAIRRNRVGLGTPNRPIGSFLFVGPTGVGKTELSKQLAIELFGSA
                     DSMIRFDMSEYMEKHSVAKLVGAPPGYVGYDEAGQLTEKVRHNPYSLILLDEVEKAHP
                     DVMHMFLQVLDDGRLTDGQGRTVSFKDAIIIMTSNAGTGKTEASVGFGAAREGRTNSV
                     LGELGNFFSPEFMNRFDGIIEFKALSKDNLLQIVELMLADVNKRLSSNNIRLDVTDKV
                     KEKLVDLGYDPKMGARPLRRTIQDYIEDTITDYYLENPSEKDLKAVMTSKGNIQIKSA
                     KKS"
     gene            complement(2562..2792)
                     /locus_tag="VYN69_001198"
     CDS             complement(2562..2792)
                     /locus_tag="VYN69_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEV1533473.1"
                     /translation="MESHLVRIINRLEAMANDGGNLKRNFEREGVVVAEVAYSHDEEN
                     GPIFTLRDVEDRETYTFDSIDLIAMEIYELLY"
     gene            2982..3665
                     /locus_tag="VYN69_001199"
     CDS             2982..3665
                     /locus_tag="VYN69_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001011621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1533474.1"
                     /translation="MNFSFLPKYLPYFNYGAVVTILISICVIFLGTILGVVLAFGQRS
                     KFKPLVWLANLYVWIFRGTPMMVQIMIAFALMHINAPTIQIGILGVDFSRLIPGILII
                     SMNSGAYVSETVRAGINAVPKGQLEAAYSLGIRPKNAMRYVILPQAIKNILPALGNEF
                     ITIIKDSSLLSAIGVMELWNGATTVSTTTYLPLTPLLFAAFYYLIMTSILTVALKAFE
                     KHMGQGDKK"
     gene            3665..4399
                     /locus_tag="VYN69_001200"
     CDS             3665..4399
                     /locus_tag="VYN69_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000140960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1533475.1"
                     /translation="MTETLIKIENLHKSFGKNEVLKGINLEIKRGEVVVIIGPSGSGK
                     STLLRSMNLLEEATKGKLIFEGVDITDKKNDLFAMREKMGMVFQQFNLFPNMTVMENI
                     TLSPIKTKGESREVAEKRAQELLEKVGLPDKADAYPQSLSGGQQQRIAIARGLAMESD
                     VLLFDEPTSALDPEMVGEVLAVMQDLAKSGMTMVIVTHEMGFAREVADRVIFMADGVV
                     VEDGTPEQIFEQTQEQRTKDFLSKVL"
     gene            4553..5410
                     /locus_tag="VYN69_001201"
     CDS             4553..5410
                     /locus_tag="VYN69_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEV1533476.1"
                     /translation="MTQIIDGKALAVKLQGQLAEKTAKLKEETALVPGLVVILVGDNP
                     ASQVYVRNKERSALAAGSRSEVVRVPETITQEELLDLIAKYNQDPAWHGILVQLPLPK
                     HIDEEAVLLAIDPEKDVDGFHPLNMGRLWSGHPVMIPSTPAGIMEMFHEYGIDLEGKN
                     AVVIGRSNIVGKPMAQLLLAKNATVTLAHSRTHNLAKVAAKADILVVAIGRAKFVTAD
                     FVKPGAVVIDVGMNRDENGKLCGDVDYEAVAPLASHITPVPGGVGPMTITMLMEQTYQ
                     AALRTLDRK"
     gene            5552..6424
                     /locus_tag="VYN69_001202"
     CDS             5552..6424
                     /locus_tag="VYN69_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEV1533477.1"
                     /translation="MKVIIQTLLEKVIIERSRSSHKGDYGRLLLLGGTYPYGGAIIMA
                     ALAAVKSGAGLVTVGTDRENIPALHSHLPEPMAFSLQDQQLLKEQLEKAEVVLLGPGL
                     GDNAFGEDLVKQVFAGLKQNQILIVDGGALTILARTSLSFPSSQLILTPHQKEWEKLS
                     GITIEKQKEDATASVLTSFPQGTILVEKGPATRIWEVGQSDYYQLQVGGPYQATGGMG
                     DTLAGMIAGFVGQFRQASLYERVAVATHLHSAIAQELSQENYVVLPTEISRYLPKIMK
                     IICQQDRVSKDKLV"
     gene            6706..7389
                     /gene="rpiA"
                     /locus_tag="VYN69_001203"
     CDS             6706..7389
                     /gene="rpiA"
                     /locus_tag="VYN69_001203"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEV1533478.1"
                     /translation="MENLKKMAGIKAAEFVSDGMVVGLGTGSTAYYFVEEIGRRIKEE
                     GLQITAVTTSSVTTKQAEGLNIPLKSIDQVDFVDVTVDGADEVDSQFNGIKGGGGALL
                     MEKVVATPSKEYIWVVDESKLVEKLGAFKLPVEVVQYGAEQVFRHFERAGYKPSFREK
                     DGQRFVTDMQNFIIDLALDVIENPIAFGQELDHVVGVVEHGLFNQMVDKVIVAGRDGV
                     QISTSKKGK"
     gene            7403..8614
                     /locus_tag="VYN69_001204"
     CDS             7403..8614
                     /locus_tag="VYN69_001204"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEV1533479.1"
                     /translation="MSKFNRIHLVVLDSVGIGAAPDANNFVNAGVPDGASDTLGHISK
                     TVGLNVPNMAKIGLGNIPRETPLKTVAAEINPTGYATKLEEVSLGKDTMTGHWEIMGL
                     NITEPFDTFWNGFPEEILTKIEEFSGRKVIREANKPYSGTAVIDDFGPRQMETGELII
                     YTSADPVLQIAAHEDIIPLDELYRICEYARSITLERPALLGRIIARPYVGEPGNFTRT
                     ANRRDLAVSPFSPTVLDKLNEAGIDTYAVGKINDIFNGAGINHDMGHNKSNSHGIDTL
                     LKTMGLAEFEKGFSFTNLVDFDALYGHRRNAHGYRDCLHEFDERLPEIIAAMRENDLL
                     LITADHGNDPTYAGTDHTREYIPLLAYSPAFKGNGLIPVGHFADISATVADNFGVETA
                     MIGESFLDKLV"
     gene            8616..9173
                     /locus_tag="VYN69_001205"
                     /pseudo
     CDS             8616..9173
                     /locus_tag="VYN69_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199123.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
     gene            9173..9982
                     /locus_tag="VYN69_001206"
     CDS             9173..9982
                     /locus_tag="VYN69_001206"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1533480.1"
                     /translation="MTFLNKIHETATFLKEKGIAAPEFGLILGSGLGELAEEIENPVV
                     VDYAEIPNWGRSTVVGHAGKLVYGELAGRKVLALQGRFHFYEGNPLEVVTFPVRVMKV
                     LGCEGVIVTNAAGGIGFGPGTLMAISDHINMTGQNPLMGENLDDFGPRFPDMSRAYTP
                     EYRATAHEVAKKLNIKLDEGVYIGVTGPTYETPAEIRSYKTLGADAVGMSTVPEVIVA
                     AHSGLKVLGISCITNFAAGFQEELNHEEVVEVTERVKGDFKGLLKAILAEL"
     gene            10458..10853
                     /locus_tag="VYN69_001207"
     CDS             10458..10853
                     /locus_tag="VYN69_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topology modulation protein"
                     /protein_id="HEV1533481.1"
                     /translation="MLDSKEELKESENDAPKLETPLREEPRLAPQTLPEASEVLENKR
                     EESKVEIIEPAQADDIRKVVGELAKDISITKLYMTGHSLGCYLAQIAAVEAYQKYPDF
                     YNHVLRKVTTFSAPKVITSRTVWDAKNGF"
     gene            10863..11438
                     /locus_tag="VYN69_001208"
     CDS             10863..11438
                     /locus_tag="VYN69_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000445853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="discoidin domain-containing protein"
                     /protein_id="HEV1533482.1"
                     /translation="MESRKLAVSGKIKHYVVDNDNVVTPLIHNNRDIVTFTGNSRFKH
                     RSRGYFESPMNDIPNFNIGKQATLDKHGYRDPKLDKVRFFKKQALPQSSSQPSAEPME
                     NIALGKQVTQSSTAFGGDARRAVDGKVDGNYGHNSVTHTNFQSKPWWQVDLAKEETIR
                     QINIYNRTDTAQDRLANFDVILLDSSGKEIE"
     gene            11463..11975
                     /locus_tag="VYN69_001209"
     CDS             11463..11975
                     /locus_tag="VYN69_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001275913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin"
                     /protein_id="HEV1533483.1"
                     /translation="MSAQITINHKKARYVRIGLEGYNALSLAEVEVFRFIATNAETAT
                     QVSKPVQPISQTPVKDKTLTIQHSGAYIARYSITWEEVTVDKDGNQVVRSHSWEGNGR
                     NQTAGFVLNLPIKENMRNLRVKIEKKTGLLWNRWQTIYENRPILAQPHRKITHWGTTL
                     NSKVSDDDVL"
     gene            12633..13343
                     /gene="deoD"
                     /locus_tag="VYN69_001210"
     CDS             12633..13343
                     /gene="deoD"
                     /locus_tag="VYN69_001210"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEV1533484.1"
                     /translation="MSIHIAAQQGEIADKILLPGDPLRAKFIAENFLDDAVCFNEVRN
                     MFGYTGTYKGHCVSVMGTGMGMPSISIYARELIVDYGVKKLIRVGTAGSLNEEVHVRE
                     LVLAQAAATNSNIVRNDWPQYDFPQIASFDLLDKAYHIAKKLGMTTHVGNVLSSDVFY
                     SNYFEKNIELGKWGVKAVEMEAAALYYLAAQYHVDALAIMTISDSLVNPDEDTTAEER
                     QNTFTDMMKVGLETLIAE"
     gene            complement(13366..13890)
                     /locus_tag="VYN69_001211"
     CDS             complement(13366..13890)
                     /locus_tag="VYN69_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topology modulation protein"
                     /protein_id="HEV1533485.1"
                     /translation="MKIAIIGYSGSGKSTLAEKLSNYYSIPKLHMDTLQFQPGWQDSD
                     HEWMLTDIKNFLTKHKAWVIDGNYSWCCYQERMQEADQIIFLNFSPLTCLFRTFKRYL
                     KYRGKVRESMAADCPERFDWEFIRWILWDGRSKTQKENYQKLCQEYSHKVTILRNQRE
                     LDQFLDKKRKSYNS"
     gene            complement(13943..14179)
                     /locus_tag="VYN69_001212"
     CDS             complement(13943..14179)
                     /locus_tag="VYN69_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001274003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEV1533486.1"
                     /translation="MANIKSAIKRAELNVKQNEKNSAQKSAMRTAIKAFEANPSEELF
                     RAASSAIDKAETKGLIHKNKASRDKARLSAKLAK"
     gene            complement(14247..15167)
                     /locus_tag="VYN69_001213"
     CDS             complement(14247..15167)
                     /locus_tag="VYN69_001213"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEV1533487.1"
                     /translation="MTNEFLHFEKISRQTWQSLHRKTTPPLTEEELESIKSFNDQISL
                     QDVTDIYLPLAHLIQIYKRTKEDLAFSKGIFLQRESKSQPFIIGVSGSVAVGKSTTSR
                     LLQILLSRTFTDATVELVTTDGFLYPNQTLIEQGILNRKGFPESYDMEALLNFLDRIK
                     NGQDVDIPVYSHEVYDIVPEEKQSVKAADFVIVEGINVFQNPQNDRLYITDFFDFSIY
                     VDAGVDDIESWYLDRFLKMLSLAQNDPDSYYYRFTQMPIGEVESFAHQVWISINLTNL
                     QNYIEPTRNRAEVILHKSKNHEIDEIYLKK"
BASE COUNT         4499 a         2785 c         3393 g         4587 t
ORIGIN      
        1 gtcaagaaat tacagttatt ttagtaaaaa aagaagcaaa aaaatttcat tttctactcc
       61 ttttctatag gatttatttt tctttttcag aacttttaac ttcagctttt tttggcagat
      121 ttaatctgaa tgtttccctt gctagtcata actgctttga gatctttttc gcttggattt
      181 tcaaggtagt agtcagtgat tgtgtcctca atatagtctt gaatagtccg acgaagtggg
      241 cgtgctccca tttttggatc ataacctagg tcaaccaact tttccttgac cttatcagtt
      301 acatccaaac gaatgttgtt gctagagagg cgcttgttaa catctgctag catgagctcg
      361 acaatctgaa ggaggttatc cttgctgaga gccttaaatt cgataatgcc atcaaaacgg
      421 ttcataaact ctgggctaaa gaagttaccg agttcaccga ggacagaatt ggtacgtcct
      481 tctctagcag caccaaatcc aacgctagct tcggtctttc ctgtacctgc atttgaggtc
      541 atgataatga tggcatcctt gaagctaacg gtgcgtcctt gcccatctgt caaacgacca
      601 tcgtccaaga cttgaagaaa catgtgcata acatctgggt gagctttttc cacttcatcg
      661 agaaggatga gagaatacgg attgtggcga actttttcag ttaattgacc agcttcatca
      721 tagccaacat aacctggagg agcgccgacc aacttagcca cactatgttt ttccatgtat
      781 tcactcatat caaagcgaat catactatca gcagaaccaa aaagttcgat agccagttgt
      841 ttggaaagtt ctgtcttacc gacaccagtt ggcccaacga agaggaagct tccgattggg
      901 cggttagggg taccaagtcc gacacgatta cggcgaatag ccttggcaat cttatcgact
      961 gcatcatctt gaccaataac atgagacttg agatcttcgg ctagatggat gagttgagat
     1021 tgttctttct ctttcaaatc accaacaggg atattggttt tctgctcgat aatgtgctca
     1081 atagttttct cgctgatgat aggagtatcc tggtctgtga tctttttctt ttgcatttcc
     1141 ttatacttgg caatctggtc gcggaagtag gccgccttct caaaatcttc ttctcgtgta
     1201 gcttgagact tgagattttc agcctcaatc aagcgctgat caattacttt aggatccaca
     1261 aaattcaagg tcaagttcat cttagaacca gcttcatcta ggaggtcaat ggccttgtca
     1321 ggcaagaagc gatcttggat gtagcgattg gaaagagttg cagctgcttc aatcgcagca
     1381 tctgtatatt gaacgtggtg gtaatcttcg tatttctttt gaatcccttt gagaatagtg
     1441 attgtttcgt ccaccgttgg ttcatcgact ttaacaggct gcatacgacg ctcgagggca
     1501 gcatcctttt caatgatacg gtattcattg agggtagtag caccgactag ttgcagttct
     1561 ccacgagcaa gggctggctt gaggatattt cctgcgtcca tattaccatc actcgcagaa
     1621 ccagcaccaa caatttcatg gatttcatcg ataaagagga tgatatcttc acgtttgcga
     1681 atttcttcca tgagtttttg catgcgttct tcaaattgtc ctcgaatccc cgttccttga
     1741 actaagctaa ccacatccag acggatgact tgtttacctt ggagtttatg tggcacatcg
     1801 ccatcgacaa ttttctgagc tagaccttcg acaacggccg tttttccgac accaggttca
     1861 ccgataagga caggattatt cttggttcta cgattgagaa tctcgatgac acggataatc
     1921 tcatcgtcgc gcccaataac ggggtcaatg tctccacgac gggcaatttc agttacatta
     1981 ataccaaatt cttccagcag gcctttttct tggctaggtg gcggagtttg agcagatcca
     2041 cgattttggg aaccataacc gccgtttcca ccgtaacctc cacctgattg ggttggggga
     2101 ataggaggag tattgctaga aggtctgaaa ttgtttagat cattgaagaa atcaccaaag
     2161 ggatcgaagt cacgattgtt cagatccgtc atacctttga agaggctatt gttaggatct
     2221 gttttgataa tcttatagca gttttgacag aggtcaattt gtttttgttt tccattgaga
     2281 ttggtgtaaa gatgaattgt tgagtcgtta attttacagt tttgacaaag catacatcta
     2341 cctcatttct ttctttagcc tgacttgttt aaaggtcaaa aatagtcaaa ttctatactc
     2401 tcattataac aggattggag tgtaaagcaa gtaaaaagat tgaagctttg agaagttgtt
     2461 acaatgaaaa tttttgtaaa tttggactat aaaaaaatcc tatttgtgag acaaaaagga
     2521 ttttacttag acatgcaggg acaatcccag cttgttttag attagtagag aagttcgtaa
     2581 atttccattg cgattaagtc aatgctatca aacgtatacg tttcgcgatc ttctacgtca
     2641 cgaagggtaa agattgggcc gttttcttcg tcgtggctgt acgcaacttc tgcaacaaca
     2701 actccttcgc gttcaaaatt acgttttaaa tttccgccat cgtttgccat tgcttcaagg
     2761 cggttgatga ttctaaccaa atgtgattcc attttgattc tccttcattt cttatcgtta
     2821 ctattttacc ataaaggagg ccaacttgac tagaaaaaac taagatttct cgctaattct
     2881 gctagtttaa agatttttaa ataaattgga taaaaaattt aaattttaat tcaaaacatg
     2941 ttataatcat acagtattct attttagaaa gcagtgtgac tatgaatttt tcttttttac
     3001 ctaagtattt accttatttt aactatgggg ctgttgtgac gattcttatt tctatctgtg
     3061 ttatcttttt gggaactatt ttgggtgttg tcttggcttt tgggcaacgt tcaaagttta
     3121 aaccgcttgt ttggttggcc aacttgtacg tttggatttt ccgtgggaca ccgatgatgg
     3181 ttcaaattat gattgccttt gctcttatgc atatcaatgc tccgactatt cagattggaa
     3241 ttttaggtgt tgatttttcg cgtctgattc cagggatttt gattatctct atgaatagtg
     3301 gtgcttatgt ttcggagact gttcgtgccg gaatcaatgc ggttccaaaa ggtcagctag
     3361 aggcggctta ttcgctaggg attcgtccta aaaatgcgat gcgttatgtg attttgccac
     3421 aagcaatcaa aaatatcttg ccagcattgg ggaacgaatt tatcaccatt atcaaggaca
     3481 gctccctctt atcagctatt ggggtcatgg agttgtggaa tggggctaca acagtttcta
     3541 caacaaccta tctaccttta acaccgcttt tatttgcagc attttattac ttgattatga
     3601 cctctattct gacagtagcc ttgaaagctt ttgaaaaaca tatgggacaa ggagataaga
     3661 aataatgaca gaaaccttga taaaaattga aaatttacat aaatcttttg gaaagaatga
     3721 agtattgaag ggcatcaacc tcgagattaa aagaggagaa gttgtcgtta tcatcggtcc
     3781 ttcagggagt ggaaaatcta ccttgcttcg ctctatgaat ttgttggagg aagcaaccaa
     3841 ggggaagctt atctttgagg gagtcgatat tacggacaag aagaatgacc tgtttgccat
     3901 gcgtgagaag atgggcatgg ttttccaaca atttaacctc tttcctaata tgactgtgat
     3961 ggaaaatatt accttgtcac ctatcaagac taaaggtgaa agcagggaag ttgctgagaa
     4021 gagagcccaa gagcttttgg aaaaagttgg tttgccagat aaggcagacg cttatccaca
     4081 gagtctgtcg ggtgggcaac aacaacggat tgccatcgcg cgtgggttgg ctatggaatc
     4141 agatgttttg ctctttgacg aaccaacttc agccttggat cctgaaatgg taggtgaggt
     4201 attggctgtt atgcaagacc ttgccaagtc agggatgact atggttatcg taacacatga
     4261 gatgggattt gcccgtgagg tggcagatcg tgttatcttt atggcagacg gtgtggttgt
     4321 tgaagatggg acacctgagc agatttttga acaaacccaa gaacaacgga ctaaagattt
     4381 cttgagtaag gttttataat ctattttaaa atttcaagac aagattagtg aaaagttcaa
     4441 ccagagtttt ttcttatagt ttgaaactat aggataacct aggaaagaag tgttagaggg
     4501 cactagcagt ttttggtata atggaagata tttgaaagaa aagagaagtg atatgacaca
     4561 gattattgat gggaaagctt tagcggtcaa attgcagggg cagttggctg aaaagactgc
     4621 aaaattaaag gaagaaacag ctctagtgcc tggtttggta gtgattttgg ttggggataa
     4681 tccagccagc caagtctacg ttcgcaacaa ggagaggtca gctcttgcgg ctggttcccg
     4741 tagcgaagta gtgcgagttc cagagaccat tactcaagag gaattgttag acctgattgc
     4801 taaatataat caggatccag cttggcatgg gattttggtc cagttgccat taccaaaaca
     4861 tatcgatgaa gaggcggttt tattagccat tgacccagaa aaggatgtgg atggtttcca
     4921 tcccctaaac atgggacgtc tttggtctgg acatccagtc atgattccct cgacacctgc
     4981 aggaattatg gaaatgtttc atgaatatgg gattgacttg gaaggtaaaa atgcggtcgt
     5041 aatcggtcgt tcaaatatcg ttggaaaacc tatggctcag cttcttttgg ctaaaaatgc
     5101 gacagtaacc ttggcccact cacgaactca taatcttgcc aaggtggctg ctaaagcaga
     5161 tattcttgta gtcgcaatcg gccgcgccaa gtttgtgact gctgactttg tcaaaccagg
     5221 tgcggttgtc attgacgttg ggatgaatcg agatgaaaat gggaagctct gtggagatgt
     5281 tgattatgag gcagttgcac cacttgctag ccatatcacg ccagtccctg gaggtgtcgg
     5341 tcctatgacc attaccatgc tgatggagca aacttatcaa gcagcacttc ggacattgga
     5401 tagaaaataa gagaaacatt cgattaatag agtgtatttt ctatagctat atctaaaatg
     5461 gcagaaatga atattaaatt ttagatataa gattacaaaa ggaggtctgc gtctcctttt
     5521 tattgtataa taaaagtgag aggaaaaaag gatgaaagtg attattcaaa ccttattaga
     5581 aaaagttatt attgaacgtt ctcgttcaag tcataaagga gattatggtc gtctgctgct
     5641 gcttggtggt acttatcctt atggaggtgc catcatcatg gctgctttag cagctgtaaa
     5701 aagcggtgca ggattggtga ccgttggaac ggacagggaa aatatcccgg ctctgcacag
     5761 ccatttacct gagcctatgg ccttttctct tcaagaccag caattgttaa aagagcaatt
     5821 ggagaaggca gaagttgtct tgctggggcc tggtttagga gacaatgctt ttggagaaga
     5881 tttagttaaa caggtctttg ctggcttaaa acagaatcag attttgattg tagacggagg
     5941 tgccttgacc attcttgcta ggacaagttt gtcatttccc tcaagccagc ttatcctaac
     6001 tcctcaccaa aaagaatggg aaaaattatc tggaattacg attgaaaagc aaaaggaaga
     6061 tgcaacggct agtgttctga cttccttccc tcaaggaaca attttggtgg agaaaggccc
     6121 agctactcgt atttgggaag ttggccaatc tgattattac cagttacagg ttggtggtcc
     6181 ttatcaggcg actgggggaa tgggagatac tctggctggg atgatcgcag gatttgtagg
     6241 ccaattccga caggccagtc tctacgagcg tgtggcagta gcgacccatc ttcattcagc
     6301 aatagcccaa gaactatctc aagaaaatta tgtggtctta ccgacggaaa tcagcaggta
     6361 tcttcccaaa ataatgaaaa ttatttgtca acaagatagg gtaagcaaag ataaacttgt
     6421 ttaaaataga cagataaatc tacttccttc tcttaattta tgattcttga aatgcgcctg
     6481 ctttaaggct aaactagacg tatcaaagtt aagagtttgt ttttgtaact ggaaagagat
     6541 gaacaaatca aatacataaa agataaactc atttttattc gtttggtaaa aataaaatgc
     6601 atatttttaa agaaagattg atgaaaaatg tagatttgat gtttttactt gaataaaaag
     6661 cggtttcata gtaaaataag aactgagaat gatggaggta aatcggtgga aaatctgaag
     6721 aaaatggcag gtatcaaggc tgctgagttc gtgagcgatg gaatggtcgt tggacttgga
     6781 acaggctcga ctgcctatta ttttgtcgaa gaaatcggtc gtcgtatcaa ggaagaagga
     6841 ttgcagatta cagctgtaac aacttctagt gtgaccacta aacaggcaga aggccttaat
     6901 atcccgctca agtctattga ccaagtagac tttgtcgatg tgacggttga tggcgcggat
     6961 gaagtggata gtcagtttaa tggaatcaaa ggcggtggtg gtgcccttct catggaaaag
     7021 gtggtcgcaa caccatcaaa agaatacatt tgggtggtgg atgaaagcaa gctggtcgaa
     7081 aaactaggtg cttttaaatt gccagtagaa gtggttcagt atggtgcaga gcaggtcttt
     7141 cgtcattttg aacgagctgg ctacaaacca agtttccgtg aaaaagacgg ccaacgtttt
     7201 gtgacggata tgcagaattt tatcattgac ctcgccttgg atgtcattga aaatccaatt
     7261 gcttttggac aagaattgga ccatgtcgtt ggtgttgtgg agcatggttt attcaaccaa
     7321 atggtggata aggtaatcgt tgctggacga gatggagttc agatttcaac ttcaaaaaaa
     7381 ggaaaataga agggggcata agatgtctaa atttaatcgt attcatttgg tggtactgga
     7441 ttctgtagga atcggtgcag caccagatgc taataacttt gtcaatgcag gggttccaga
     7501 tggagcttct gacacactgg gacacatttc aaaaacagtt ggtttgaatg tcccaaacat
     7561 ggctaaaata ggtcttggaa atattcctcg tgaaactcct cttaagactg tagcagctga
     7621 aatcaatcca actggatatg caacaaaatt agaggaagta tctcttggta aggatactat
     7681 gactggacac tgggaaatca tgggactcaa cattactgag cctttcgata ctttctggaa
     7741 cggattccca gaagaaatcc tgacaaaaat cgaagaattc tcaggacgca aggttattcg
     7801 tgaagccaac aaaccttatt caggaacggc tgttatcgat gattttggac cacgtcagat
     7861 ggaaactgga gagttgatta tctatacttc agctgaccct gttttgcaga ttgctgccca
     7921 cgaagacatt attcctttgg atgaattgta ccgtatctgt gaatacgctc gttcgattac
     7981 ccttgagcgt cctgcccttc ttggtcgcat cattgctcgc ccttatgtag gtgaaccagg
     8041 taacttcact cgtacggcaa accgtcgtga cttggctgta tctccatttt ccccaactgt
     8101 tttggataaa ttgaatgagg ctggtatcga tacttatgct gtgggtaaaa tcaacgatat
     8161 ctttaacggt gctggtatca accatgacat gggtcacaac aagtcaaata gtcatggaat
     8221 tgatacacta ttgaagacta tgggacttgc tgagtttgaa aaaggattct cattcacaaa
     8281 cctagttgac tttgatgccc tttacggcca tcgtcgtaat gctcacggtt accgtgattg
     8341 tttgcatgag tttgatgaac gcttacctga aattatcgca gctatgagag agaatgacct
     8401 tctcttgatt actgcggacc atggaaatga cccaacgtat gcagggacgg atcacactcg
     8461 ggaatatatt ccattgttgg catatagccc tgcctttaaa ggaaatggtc tcattccagt
     8521 aggacatttt gcagatattt cagcgactgt tgccgataac tttggtgtgg aaactgctat
     8581 gattggggaa agtttcttag ataaattggt ataagatgac gcgctatgct ttgctggtga
     8641 gaggtatcaa tgttggtagt aagaataagg tcgttatggc ggagcttcgt caagaattga
     8701 caaacttggg actggaaaag gttgagagct acatcaacag tggcaatatt ttctttactt
     8761 cgatagattc caaagcccaa ttggtttaaa agctagagac tttctttgca gtccattatc
     8821 catttattca gagcttttct ttactgagtc tagaggactt tgaggcggaa cttgaaaatc
     8881 taccagcttg gtggagcaga gacttggcac gaaaagattt tctcttttac actgagggtt
     8941 tggatgtgga ccaagtcatc gcgacagttg aaagtttaga gctgaaagat gaagtgcttt
     9001 attttagaaa acttgggatt ttctggggga aattttctga agaatcctat tctaagactg
     9061 cctatcataa gtacttgctg aaggtgcctt tctaccgcca cattactatt cgtaatgcta
     9121 aaacctttga caaaattggt caaatgctaa aaaataataa aggagacaca taatgacatt
     9181 tttaaacaaa atccatgaaa ctgctacttt cctgaaagaa aagggaattg cagcccctga
     9241 gttcggtcta atccttgggt caggacttgg agaattggca gaagaaatcg aaaatccagt
     9301 tgtagtagac tatgctgaga ttccaaactg gggccgttca acagtagtcg gtcatgctgg
     9361 taaattggta tatggtgaac tggcaggtcg caaggtcttg gctcttcaag ggcgtttcca
     9421 tttctatgaa gggaatcctc tggaagtggt gactttccca gttcgtgtga tgaaagttct
     9481 tggatgtgaa ggtgttattg taaccaatgc agctggcggt atcggatttg gtcctggtac
     9541 cttgatggct atctcagacc atatcaacat gacggggcaa aatccattga tgggtgaaaa
     9601 cttggatgac tttggcccac gtttcccaga tatgtctagg gcctacacac cagaataccg
     9661 tgccactgcc catgaagtgg ctaaaaaact taatatcaag cttgatgaag gtgtctatat
     9721 cggagttact ggtccgactt atgaaacacc agcagaaatt cgttcctata agacactggg
     9781 agcagatgca gttggtatgt ctacggttcc tgaagttatc gtggcggccc actctggctt
     9841 gaaagttctg ggaatttcat gtatcactaa ctttgcggcc ggtttccaag aagaactcaa
     9901 tcacgaagaa gttgtagaag tgactgaacg tgttaaaggt gatttcaaag gcttgcttaa
     9961 agcgattctt gctgaattgt aagaaaaaag atttaaaagg gggagtgcct ctgttttttc
    10021 aggattgact gcctatccgg attaaagaag aaacagagga atactatgag cttcttcctg
    10081 ctcttataac tgaaagaagc ggaagaatag gtgtgtctga tctgatagcc agcattgtga
    10141 aagacaagat tctaggatac tagcattagc ttcctagcca agtagactag tatgataagg
    10201 agagatgaga atgaattgac tttctgaatt tctcagtctt atcatatata gcacaatgag
    10261 atttcgcttg agtctgcttg taaataaacg aaaagaaaga taagaaataa tgaaaattgg
    10321 tcaacgaatt atgcgctttg gcataaaaaa ttaagtatcg gagttgtatc tgttgtagtc
    10381 ggctttgatt tctagctcca gctggaattt cagccaatga agtaaagcaa gatgtaacat
    10441 ctgaagtggt aataggtgtg ctagattcta aggaggaatt gaaagagtca gaaaatgatg
    10501 ctccaaaact agaaactcct cttagagagg agccaagact agctcctcaa acgcttccgg
    10561 aagcaagtga agttcttgaa aacaaaaggg aagagtcaaa agtagagata atagagccag
    10621 ctcaagcgga tgatatccgc aaggttgttg gggaattagc caaggatata agtattacta
    10681 agttgtatat gacaggtcat tctcttggat gttacctagc tcagattgca gcggttgaag
    10741 cttaccaaaa atatcctgat ttttataacc atgtattgag gaaagtgaca actttcagtg
    10801 ctcctaaagt gattacttcc agaactgttt gggatgctaa gaatggtttc tgagatgttg
    10861 gtttggaaag tcgtaaatta gctgttagtg gaaaaattaa gcattatgtg gttgataatg
    10921 acaatgttgt gactcccttg attcataata atcgtgatat tgttacattt acaggtaatt
    10981 cacgctttaa acaccgttct cgtggctatt ttgaaagtcc aatgaatgat attcctaact
    11041 ttaatattgg taaacaagct accttggata aacatggtta tcgtgatccg aaattggata
    11101 aagtgcgatt ctttaagaaa caggctctac ctcaatcttc tagtcaacca agcgctgaac
    11161 caatggaaaa tattgcctta ggaaaacagg ttactcaaag ttcgacagct ttcggaggag
    11221 atgctagaag agctgtggat ggcaaagtcg atggtaacta tggtcacaat tctgtcactc
    11281 atacaaactt ccaatctaag ccttggtggc aagtagattt ggctaaagaa gaaaccattc
    11341 gccaaatcaa tatttacaac cgaacagaca ctgcccagga tagattggca aactttgatg
    11401 tcattctttt agacagttct ggtaaagaaa ttgagtgaaa acgtataata tctcctaaag
    11461 atgtgtcagc acaaattacg attaaccata aaaaagcgcg ctatgttcgg attgggctag
    11521 aaggctataa tgccctcagt cttgcagaag ttgaagtttt ccgctttata gctacgaatg
    11581 ctgaaacggc gacacaagtt tctaagccag ttcaaccaat cagtcagact cctgtgaaag
    11641 ataaaacatt gacaattcaa cacagtggag cttacattgc ccgctactcc ataacttggg
    11701 aagaagttac agtagataaa gatggaaacc aagttgttcg tagtcattct tgggaaggaa
    11761 acggtcgcaa ccagactgca ggttttgtcc tcaacctccc aatcaaagaa aatatgagaa
    11821 atctgcgagt taagattgag aaaaagacgg gcctactatg gaatagatgg caaacaatct
    11881 atgaaaacag accaatttta gctcaacccc accgtaaaat tacccattgg ggtacgacat
    11941 tgaattccaa ggtgagtgac gatgatgtct tgtaatctga tggtacaatg acagttagtt
    12001 tgtctagttt ataagaaagt actacctgag cttgaataga actcaggtag ctctctgtga
    12061 aagaacaaaa ttaatactca atgaaaatca aagagcaaac taagaaacta gccgcaggtt
    12121 gctcaaagca ctgctttgag gttgtagata agactgacga agtcagtcac atatataatc
    12181 caaggcgacg ttgacgtggt ttgaagagat tttcgaagag tataaacaga aaggtagagc
    12241 gcgtgttcta atttgaacac gagtagaaaa cttttctaaa aataaaaacg aaaggatggg
    12301 taaactgtat tcgctgaact gaatacgggc gactctcctc taaatcaaaa ttaagaaaga
    12361 aaggaattga ccccacccta aaagcagtgg gaaaaagata gttggtctag cgagcatcgc
    12421 tcactgcgcc caactcctat tttcccttcg ctttttgatg ggtttggtat ctttctcaat
    12481 ataaaatata aaataaagaa aggtagagcg tgtgttttga tttgaacacg agcggaaaac
    12541 tcggaaaata gataatctga ctgaaaaatc aggatttctc gtcaggttcc taattttcag
    12601 tcgttttctt ctcgctcttt gtatcataaa ttatgtctat ccatattgct gctcagcagg
    12661 gtgaaattgc tgataaaatt cttcttcctg gggatcctct tcgtgctaag tttattgcgg
    12721 agaatttcct tgatgatgct gtttgtttta acgaagtgcg taacatgttt ggttacactg
    12781 gtacttacaa gggtcactgt gtatctgtca tgggaactgg gatgggaatg ccatctattt
    12841 cgatttatgc gcgtgagtta atcgtagact acggtgtgaa gaaattgatt cgtgtgggaa
    12901 ctgcaggttc tttgaatgaa gaggttcatg ttcgtgaatt agttttggcg caggcggctg
    12961 caaccaactc aaacatcgtt cgtaatgact ggccacagta cgattttcca caaattgcta
    13021 gctttgattt gcttgataaa gcctaccata tcgccaaaaa acttggtatg actactcacg
    13081 ttgggaacgt tttgtcatct gatgtctttt actcaaatta ctttgaaaag aatatcgagc
    13141 ttggtaaatg gggagtcaag gctgtggaaa tggaagcagc agctctttac tatcttgctg
    13201 cccaatacca tgttgatgcg ctagctatca tgaccatctc tgatagcttg gtcaatccag
    13261 acgaagacac aactgcagaa gaacgtcaaa ataccttcac tgatatgatg aaggttggtt
    13321 tggaaacctt gattgcagaa taattatagc caaaaaggaa gccctttatg aattgtagga
    13381 cttccttttc ttatccagaa attgatctag ctctctctga tttcgaagaa tagtgacttt
    13441 atgtgaatat tcttggcaaa gtttttggta attttctttt tgagttttgc tacgcccatc
    13501 ccaaagaatc catctgataa actcccagtc aaagcgttca gggcaatctg ccgccatact
    13561 ttctctgact tttccacggt atttaagata acgcttaaag gttctaaaga gacaggtcaa
    13621 tggcgaaaaa ttgagaaaga tgatttggtc agcttcttgc attcgttctt ggtagcagca
    13681 ccaagaataa ttaccatcga tgacccaagc tttatgcttg gtgagaaagt tttttatatc
    13741 ggttaacatc cattcgtggt cactgtcttg ccaaccaggt tgaaattgga gtgtgtccat
    13801 gtgcagtttt ggaatggagt agtagttaga taacttttct gctagagttg acttaccaga
    13861 accagaatat ccgataattg cgattttcat tttctacctt ttcctatttg gagacaaaaa
    13921 aacagcctct atggactgtt tcttatttag caagtttagc tgaaagacga gctttatcgc
    13981 ggcttgcttt gtttttgtga atcaaacctt tagtttctgc tttatcgata gctgagctag
    14041 cagcacggaa aagttcttca gatgggtttg cttcgaaagc ttttatagca gtacgcatag
    14101 ctgatttttg agctgagttc ttttcgtttt gtttaacgtt caattcagcg cgtttgatag
    14161 ctgatttaat gtttgccaat attcttacct ccatatttac taactatacc attatatatg
    14221 aaaacttacg ttttgacaag ggaaaattac ttttttaagt aaatttcatc gatttcatgg
    14281 ttcttgcttt tatgaagaat cacttctgca cgatttctgg ttggttcaat ataattttgc
    14341 agatttgtga gattgatact gatccagacc tgatgggcaa aggactccac ttccccaatc
    14401 ggcatctgag taaaacgata atagtagcta tcagggtcgt tttgggctag gctcagcatt
    14461 ttcaagaaac ggtccagata ccaactttca atatcatcca ctccagcatc tacatagatg
    14521 gaaaagtcaa agaagtcagt gatatagaga cgatcgtttt gtggattttg aaagacattg
    14581 attccctcaa caattacaaa atcagcagct ttgacacttt gtttctcttc gggtacgatg
    14641 tcgtaaactt catgagaata gacaggaata tctacatctt gtccattttt gatgcggtcc
    14701 aagaagttga gaagagcttc catatcatag ctttcaggaa atcctttacg atttaaaatc
    14761 ccctgctcaa tcaaggtttg attgggatag agaaaaccat cagttgtaac caactcaacc
    14821 gtagcatctg taaacgtacg ggacagtagg atttgaagta ggcgactggt tgtggatttt
    14881 ccaacggcaa cactcccaga aaccccaata ataaaaggtt gagatttact ttcacgttgg
    14941 aggaaaattc cttttgaaaa ggctaaatct tccttagttc gcttgtaaat ctgaatcaga
    15001 tgagccaagg ggagatagat atctgtaacg tcttggagac tgatttggtc attaaaactc
    15061 ttgatagatt ccaattcttc ttctgtcaaa ggaggtgttg tctttcgatg taaagattgc
    15121 caagtctggc ggctgatttt ttcaaaatgt aaaaattcgt tggtcatttg ttttccccta
    15181 ggtattttat ctatcatacc ataaaaagct aaaaaaataa agcggtttct tgaatgttgt
    15241 aagtcaatga tatataatat aagc
//
LOCUS       DAVLVP010000041        14826 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.041, whole genome shotgun sequence.
ACCESSION   DAVLVP010000041 DAVLVP010000000
VERSION     DAVLVP010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.041"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(90..494)
                     /locus_tag="VYN69_001214"
     CDS             complement(90..494)
                     /locus_tag="VYN69_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HEV1533488.1"
                     /translation="MKDFHFDAISAFENYEIEKMRDGHVVVTTKVVNSSLNYYGNAHG
                     GYLFTLCDQISGLVVISLGLDGVTLQSSINYLKAGKLDDVLTIKGECVHQGRTTCVMD
                     VDITNQEGRNVCKATFTMFVTGQRSEERRVRI"
     gene            complement(564..2045)
                     /locus_tag="VYN69_001215"
     CDS             complement(564..2045)
                     /locus_tag="VYN69_001215"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1533489.1"
                     /translation="MTLVDKFVTHVISESSFEEMDRIYLTNRVLARVGEGVLEVETNL
                     DKLIDLKDQLVEESVRLETIEDSQTAREILGTELMDLVTPCPSQVNRDFWEAYVHSPE
                     QAIEDFYQLSQKNDYIKLKAIAKNIAYRVPSDYGELEITINLSKPEKDPKEIVAAKLV
                     QASNYPQCQLCLENEGYHGRVNHPARSNHRIIRFEMVGQEWGFQYSPYAYFNEHCIFL
                     DGQHRPMAISRQSFERLLAIVEQFPGYFAGSNADLPIVGGSILTHDHYQGGRHVFPME
                     LAPLQKTFRFAGFEQVKAGIIKWPMSVLRLTSDSKEDLINLADKIFQEWRQYSDSSVQ
                     ILAETDGTPHHTITPIARKRDGQFELDLVLRDNQTSAEYPDGIYHPHKDVQHIKKENI
                     GLIEVMGLAILPPRLKEEVEQVASYLVGEAVTVADYHQEWADQLKSQHPDLTDKEKAL
                     AIVKDSVGAIFARVLEDAGVYKQTEQGQTAFMRFVEHVGILLD"
     gene            complement(2064..3242)
                     /locus_tag="VYN69_001216"
     CDS             complement(2064..3242)
                     /locus_tag="VYN69_001216"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEV1533490.1"
                     /translation="MAQHLTTEVLRKDFLAVFGQEADQTFFSPGRINLIGEHTDYNGG
                     HVFPAAISLGTYGAARKRDDQVLRFYSANFEDKGIIEVPLADLKFEKEHNWTNYPKGV
                     LHFLQEAGHVIDKGFDFYVYGNIPNGSGLSSSASLELLTGVVAEHLFDLKLDRLDLVK
                     IGKQTENNFIGVNSGIMDQFAIGMGADQRAIYLDTNTLEYDLVPLDLKDNVVVIMNTN
                     KRRELADSKYNERRAECEKAVEELQVALDIQTLGELDEWAVDQYSYLIKDENRLKRAR
                     HAVLENQRTLKAQAALQAGDLETFGRLMNASHVSLEHDYEVTGLELDTLVHTAWAQEG
                     VLGARMTGAGFGGCAIALVQKDTVEAFKEAVGKHYEEVVGYAPSFYIAEVAGGTRVLD
                     "
     gene            3407..4414
                     /gene="galR"
                     /locus_tag="VYN69_001217"
     CDS             3407..4414
                     /gene="galR"
                     /locus_tag="VYN69_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator GalR"
                     /protein_id="HEV1533491.1"
                     /translation="MATLKDIAQLASVSIATVSRVLNRDQSLSVTEETRHRILTVAEE
                     LGYTKHLKTGESHKPKQKIAIIQWVSEQGELDDLYYYQIRLGIEKRAQELDYDILRYF
                     NDHPFTLSEEVIGILCIGKFSRAQISAFEEYQKPLVFLDSDTLSLGHTCIITDFYTAM
                     KQVVDYFLSQGMDRIGILTGLEETTDQEEIIQDKRLENFKNYSQARGIYHDELVFQGR
                     FTAQSGYDLMKEAIQSLGDQLPPAFFAASDSLAIGALRALQEAGISLPDRVSLISFND
                     TSLTKQVYPPLSSITVYTEEMGRAGMDILNKEVLHGRKIPSLTMLGTRLTLRESTLNQ
                     E"
     gene            complement(4460..5497)
                     /locus_tag="VYN69_001218"
     CDS             complement(4460..5497)
                     /locus_tag="VYN69_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000833740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent alcohol dehydrogenase"
                     /protein_id="HEV1533492.1"
                     /translation="MKSAVYTKAGQVGLASIERPQIIEADDVIIRVVRACVCGSDLWR
                     YRNPETKAGHKNSGHEAIGIVEEAGEAITTVKPGDFVIVPFTHGCGECDACLAGFDGS
                     CDNHIGNNLGGDFQAEYIRFHYANWALVKIPGQPSDYTEGMLKSLLTLADVMPTGYHA
                     ARVANVQKGDKVVVIGDGAVGQCAVIAAKMRGASQIILMSRHEDRQKMAMESGATAVV
                     AERGQEGITKVREILGGGADVALECVGTEAAIEQALGVLHNGGRMGFVGVPHYNNRAL
                     GSTFMQNISVAGGAASATTYDKQFLLKAVLDGDINPGRVFTSSYKLEDIDQAYKDMDE
                     RKTIKSMIVIE"
     gene            complement(5498..5851)
                     /locus_tag="VYN69_001219"
     CDS             complement(5498..5851)
                     /locus_tag="VYN69_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEV1533493.1"
                     /translation="MNIKSASDLLGISADTIRYYERVGLVPPITRTATGIRDFQDQDI
                     EALEFIKCFRSAGVSVDSLVDYMSLYQKGDETREERLGILEEEKQKLEERLSQLQTAL
                     NRLNLKIKLYKEGKF"
     gene            complement(6095..6985)
                     /locus_tag="VYN69_001220"
     CDS             complement(6095..6985)
                     /locus_tag="VYN69_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEV1533494.1"
                     /translation="MKAKYAVWVAFFLNLTYAIVEFIAGGVFGSSAVLADSVHDLGDA
                     IAIGISAFLETISNREEDNQYTLGYKRFSLLGALVTAVILVTGSVLVILENVTKILHP
                     QPVNDEGILWLGIIAITINLLASLVVGKGKTKNESILSLHFLEDTLGWVAVILMAIVL
                     RFTDWYILDPLLSLVISFFILSKALPRFWSTLKIFLDAVPEGLDIKQVKSGLERLDNV
                     ASLNQLNLWTMDALEKNAIVHVCLKEMEHMETCKESIRIFLKDCGFQNITIEIDADLE
                     THQTHKRKVCDLERSYEHQH"
     gene            7118..7654
                     /locus_tag="VYN69_001221"
     CDS             7118..7654
                     /locus_tag="VYN69_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEV1533495.1"
                     /translation="MTNIDRRISKTKKAIYQAFIQLLNAKGYEATTVQDIIDLADVGR
                     STFYCHYESKELLLDQLCRYLFHHLFEREQAISTEDYLAHLFLHFHKNQDHITSLLFS
                     KNDYFLRQLHKELEHHVYSVLADNLKEAHPNMPTSYLQHLVVSNFIETLTWWLKKGQD
                     FTGQEVVQFYLDLLIPKN"
     gene            complement(7820..8626)
                     /locus_tag="VYN69_001222"
     CDS             complement(7820..8626)
                     /locus_tag="VYN69_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001863945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HEV1533496.1"
                     /translation="MHTYLQKKIENIKTTLGEMSGGYRRMVAAMADLGFSGTMKAIWD
                     DLFAHRSFAQWIYLLVLGSFPLWLELVYEHRIVDWIGMICSLTGIICVIFVSEGRASN
                     YLFGLINSVIYLILALQKGFYGEVLTTLYFTVMQPIGLLVWIYQAQFKKEKQEFVARK
                     LDDKGWTKYLSISVLWWLAFGFIYQSIGANRPYRDSITDATNGVGQILMTAVYREQWI
                     FWAATNVFSIYLWWGESLQIQGKYLIYLINSLVGWYQWSKAAKQNTDLLN"
     gene            complement(8971..10491)
                     /locus_tag="VYN69_001223"
     CDS             complement(8971..10491)
                     /locus_tag="VYN69_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEV1533497.1"
                     /translation="MTNLIATFQDRFGDWLTALSQHLQLSLLTLLLAILLAIPLAVYL
                     RYHEKLADWVLQIAGIFQTIPSLALLGLFIPLMGIGTLPALTALVIYAIFPILQNTIT
                     GLKGIDPSLQEAGIAFGMTRWERLKKFEIPLAMPVIMSGIRTAAVLIIGTATLATLIG
                     AGGLGSFILLGIDRNNASLILIGALSSAVLAIAFNFLLKVMEKAKLRTIFSGFALVAL
                     LLGLSYSPALLAQKEKENLIIAGKIGPEPEILANMYKLLIEENTSMTATVKPNFGTTS
                     FLYEALKKGDIDIYPEFTGTVTESLLQPSPKVSHEPEQVYQVARDGIAKQDHLAYLKP
                     MSYQNTYAVAVPKKIAQEYGLKTISDLKKVEGQLKAGFTLEFNDREDGNKGLQSMYGL
                     NLNVATMQPALRYQAIQSGDIQITDAYSTDAELERYDLQVLEDDKQLFPPYQGAPLMK
                     EALLKKHPELERVLNTLAGKITESQMSQLNYQVGVEGKSAKQVAKEFLQEQGLLKK"
     gene            complement(10484..11212)
                     /locus_tag="VYN69_001224"
     CDS             complement(10484..11212)
                     /locus_tag="VYN69_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533498.1"
                     /translation="MIEYKNVALRYTEKDVLRDVNLQIEDGEFMVLVGPSGSGKTTML
                     KMINRLLEPTDGNIYMDGKRIKDYDERELRLSTGYVLQAIALFPNLTVAENIALIPEM
                     KGWSKEEITKKTEELLAKVGLPVAEYGHRLPSELSGGEQQRVGIVRAMIGQPKILLMD
                     EPFSALDAISRKQLQVLTKELHKEFGMTTIFVTHDTDEALKLADRIAVLQDGEIRQVS
                     NPETILKAPATDFVADLFGGSIHD"
     gene            complement(11227..11991)
                     /locus_tag="VYN69_001225"
     CDS             complement(11227..11991)
                     /locus_tag="VYN69_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HEV1533499.1"
                     /translation="MSQQVKNAHNLYIHAIQDGRVAEAQAQSVGDTYIQHSTGVPDGK
                     EGFAAFFADFFERYPERQIKIVRTIEDGNLVFVHVHQYLNGGEAQWVTTDTFRADENG
                     RIVEHWDVIDYYRTPENDQLDQIFGDFEIKDLDKKAENKKLVRRFLTEIFQNGELEQW
                     SDYVADDLIQHNHEIGQGSAAYKNYVAEYSVTFDFVFQLLGQGNYVVSYGQTQIDGVA
                     YAKYDIFRLKNGKIVEHWDNKEVMPKVEDLTNRGKF"
     gene            complement(11995..12438)
                     /locus_tag="VYN69_001226"
     CDS             complement(11995..12438)
                     /locus_tag="VYN69_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000655204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEV1533500.1"
                     /translation="MKDGHLLAHHIRLLNGRIFQKLLSQDPEALYRGEQGKILAVLWN
                     SETGCATATDIALATGLANNTLTTMIKKLEEQKLVIVSPCGKDKRKKYLVLTELGKSQ
                     KEVGHRVSQKLDTIFYKGFSEEEIHQFEGFQERILANLKEKGNEV"
     gene            complement(13120..13416)
                     /locus_tag="VYN69_001227"
     CDS             complement(13120..13416)
                     /locus_tag="VYN69_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000230158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1533501.1"
                     /translation="MAEPNLESLIKDLYNHARHDLSEDLVAALLETAKKLPTTNEQLQ
                     AVRLSGLVNRELLLNPKHPAPELLNLARFIKREEAKYRGTATSALMYEELFKML"
     gene            complement(13438..14502)
                     /gene="pepA"
                     /locus_tag="VYN69_001228"
     CDS             complement(13438..14502)
                     /gene="pepA"
                     /locus_tag="VYN69_001228"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEV1533502.1"
                     /translation="MTTLFSKIKEVTELAAVSGHEAPVRAYLREKLTPHVDEVVTDGL
                     GGIFGIKHSEAVDAPRVLVASHMDEVGFMVSEIKPDGTFRVVEIGGWNPMVVSSQRFK
                     LLTRDGHEIPVISGSVPPHLTRGKGGPTMPAIADIVFDGGFADKDEAESFGIRPGDTI
                     VPDSSAILTANEKNIISKAWDNRYGVLMVSELAEALSGQKLGNELYLGSNVQEEVGLR
                     GAHTSTTKFDPEVFLAVDCSPAGDVYGGQGKIGDGTLIRFYDPGHLLLPGMKDFLLTT
                     AEEAGIKYQYYCGKGGTDAGAAHLKNGGVPSTTIGVCARYIHSHQTLYAMDDFLEAQA
                     FLQALVKKLDRSTVDLIKHY"
BASE COUNT         4367 a         3559 c         2630 g         4270 t
ORIGIN      
        1 ttgtatttca tcactctctt ttatatttcc ttctaacata tctcataaat taaaaatgag
       61 tgagataaat ttatcccact cacctttctt tatatcctta cccgtctttc ctcagacctt
      121 tggcctgtga caaacatggt aaaggttgct ttacagacat ttctgccctc ttgattggtg
      181 atatccacat ccatcacaca ggttgtacga ccttgatgga cacattctcc tttaatggtc
      241 aatacatcgt cgagttttcc tgccttaagg tagttgatag aggattggag tgtcacccca
      301 tcaagtccca gcgagataac caccaaacca ctgatttggt cacatagcgt gaatagatag
      361 ccaccatggg cattgccata gtagttgagc gacgagttca ctactttggt cgtcaccaca
      421 acgtgaccat ctctcatttt ttcaatttcg taattttcaa aggcagatat agcgtcaaaa
      481 tgaaagtctt tcatcgtttc ctcctgatat ttcaaacggc actatgatac tactttttat
      541 aggactgtgc aaggagaaag ctcctagtct agtaaaattc cgacatgttc cacaaagcgc
      601 ataaaggctg tctgcccttg ttctgtctgc ttgtagactc ctgcatcctc aagtacacgc
      661 gcaaagatag cacccacaga gtccttgacg attgcaaggg ctttttcttt atccgttagg
      721 tctggatgtt gggatttgag ttggtctgcc cactcctgat gataatcggc aactgtaaca
      781 gcttctccta caagatagct agcgacttgc tccacttctt ctttcagacg tggtggcaag
      841 attgccaagc ccatgacctc aatcaagccg atattttcct tcttgatatg ttggacatcc
      901 ttgtggggat gatagatacc atcaggatac tctgctgaag tctgattgtc tcgcaagacc
      961 aagtccaact caaactgtcc atcacgctta cgcgctattg gtgtgatggt gtgatgcggt
     1021 gtcccgtctg tctctgctaa aatctgcaca ctagaatctg aatactgacg ccattcctgg
     1081 aaaatcttat cagctaagtt aatcaaatcc tctttggaat ccgaagtcaa gcgtaggaca
     1141 gacattggcc acttgataat tccagccttg acctgctcaa aaccagcaaa tcggaaggtc
     1201 ttttgcaagg gagccaattc cataggaaat acgtgacggc ctccctgata atgatcatga
     1261 gttagaatag agccccccac aatcggcagg tcggcattag atccagcaaa atatcctgga
     1321 aactgctcta cgatagccaa cagacgttca aaactctgac gactaatggc catgggacga
     1381 tgctggccat ctaaaaagat acaatgctca ttaaagtaag catagggcga atactggaac
     1441 ccccattcct gaccaaccat ttcaaaacgg ataatccggt ggttgctgcg agctgggtgg
     1501 ttgacccgac catggtaacc ctcattctct aaacaaagtt gacactgagg ataattacta
     1561 gcttgcacca acttggctgc cacaatctct ttcggatcct tttcaggctt agagagattg
     1621 atggtaattt caagttctcc ataatcagat ggaacacgat aagcgatatt tttagcaatg
     1681 gccttgagtt tgatgtagtc atttttctga ctgagttgat aaaaatcctc tatcgcttgc
     1741 tcaggagagt ggacataggc ttcccagaag tcacgattga cctgactcgg acaaggagtc
     1801 accaagtcca tcaattcagt accaaggatt tcacgcgcag tctgactatc ctcaatcgtc
     1861 tctaatcgaa cggattcctc aactagctgg tccttgaggt caatcaattt atccagattg
     1921 gtctcaactt ccaaaacacc ttctcccact cgtgccaaaa cacgattggt caggtagatt
     1981 cgatccattt cctcaaatga actttcagaa atgacatgtg ttacaaattt atctactaag
     2041 gtcactatag agcctccttt tgactagtca aggacgcgag tgccacctgc aacttcagcg
     2101 atatagaagc ttggagcgta tccaactact tcctcgtagt gtttgcctac agcttcctta
     2161 aaggcctcaa cagtatcttt ttgaaccaag gcaatggcac agccaccaaa accagcccct
     2221 gtcatacgag caccgagaac tccttcttgt gcccaagctg tgtgaacaag ggtatctaat
     2281 tccaaaccag ttacttcata atcatgctcc agagaaacgt gtgacgcatt catcaaacga
     2341 ccaaatgttt ccaaatcacc tgcttgaagg gctgcttgag ctttaagggt acgttggttt
     2401 tcaagcacag catggcgagc acgtttcaaa cgattttcat ctttaattag atagctatat
     2461 tggtcaacgg cccactcgtc caattcaccc agggtctgaa tatccaaggc aacttgcaat
     2521 tcttccactg ctttttcaca ctcagcacga cgttcattgt atttagagtc cgccaattca
     2581 cggcgtttgt tggtgttcat gataacaacg acattgtcct tcaaatcaag tggcaccaaa
     2641 tcatactcta aggtgttggt atcaaggtaa atagcacgtt gatcagcccc cataccaata
     2701 gcaaactggt ccatgatacc agagttgact ccgataaagt tattttctgt ttgttttccg
     2761 attttaacca aatcgagacg gtctagtttt aaatcaaaga gatgctctgc cacgactcct
     2821 gtcaagagct ccaaggatgc tgaagaagac aagccagaac catttgggat attcccataa
     2881 acataaaaat caaaaccttt gtcaatcacg tgcccagctt cttgcaagaa atgaagaacc
     2941 ccttttggat agttggtcca gttgtgctct ttttcaaact tgaggtcagc gagaggcact
     3001 tcaattatgc ccttgtcttc aaagttagct gagtagaaac gtaaaacttg gtcgtcacgc
     3061 ttgcgagctg caccataagt tcccaaggaa atagcagctg gaaaaacgtg cccaccgttg
     3121 tagtctgtgt gttcaccaat caaattaata cggcctggtg aaaagaaggt ttggtctgct
     3181 tcttgaccaa aaacagcaag aaagtctttg cgaaggactt cagtagtaag atgttgtgcc
     3241 atatgaattc tcctttgact gtccgttaag ccatcttaac gtgtaatcgt tttctttcta
     3301 ttgtatccaa ggaatttccc aaggtcaata ctttttacta aaattttagt aaaatatcag
     3361 taaaaagaag aaaaacatga tatactaacc taatcaagga ggatttatgg ctaccttaaa
     3421 agacattgca cagctagcct ctgtctctat cgcgaccgta tcccgcgtcc tcaatcgcga
     3481 ccagagccta tctgttacag aagaaaccag acaccgtatt ttaaccgttg ctgaagagct
     3541 gggctacacc aagcacctca agacaggcga gtcccacaaa cccaagcaaa agattgccat
     3601 tattcaatgg gtcagcgaac aaggggagct ggacgacctc tactactacc agattcgcct
     3661 aggaatagaa aaaagagccc aagagttgga ctatgatatc ttgcgctatt ttaatgacca
     3721 tccttttacc ttaagcgagg aagtgattgg gattctctgc atcggaaaat ttagccgagc
     3781 tcagatttct gcctttgaag aataccaaaa acccctggtc tttctagaca gtgatacact
     3841 ttccctggga catacctgca ttattacgga cttttacact gctatgaaac aggttgtcga
     3901 ttatttcctc agtcaaggga tggaccgcat cgggattcta acagggcttg aggaaacaac
     3961 agaccaagaa gaaatcattc aggacaagcg tctagaaaac ttcaaaaact acagtcaagc
     4021 gaggggaatc tatcatgatg aactggtctt tcaaggaaga tttactgccc agtctggcta
     4081 tgacttaatg aaggaggcca ttcagagctt gggagaccaa cttccgccag catttttcgc
     4141 agccagcgat agtttagcta tcggtgccct ccgtgccctc caagaagctg gaatcagcct
     4201 gccagatcgc gtcagcctca tttcattcaa cgacaccagt ctgaccaaac aggtctatcc
     4261 tcccctctct agtattacag tttatactga agaaatgggg cgagcaggta tggatattct
     4321 taacaaagaa gtcctccacg gtcgcaaaat tcctagcctg accatgctgg gaaccagact
     4381 gacattaaga gaaagtaccc taaatcaaga ataggataac ataaaaaacg aatagagttc
     4441 taaaactcct attcgttttt tattcgatta caatcataga cttaattgtc ttacgttcat
     4501 ccatatcttt ataggcttgg tcgatatctt ccagtttata acttgaagta aagacgcgac
     4561 ctggattgat atcaccatca aggacggctt ttagtaaaaa ttgcttatcg tatgttgtag
     4621 cagaagctgc cccacctgct acagagatat tttgcataaa tgtcgaacca agagcacgat
     4681 tattatagtg tgggactcct acaaagccca tacgccctcc attatgaaga acacctagcg
     4741 cctgttctat agcagcctcc gtaccaacac attcaagtgc tacatctgct cctccaccga
     4801 ggatttcacg caccttggta attccttctt gaccacgttc tgcaacaaca gctgtcgcac
     4861 ctgactccat agccatcttt tgacggtctt catgacggct cataaggata atttgtgatg
     4921 ctccacgcat cttagccgcg atgacagcac attgaccaac agccccatca ccgataacaa
     4981 caaccttgtc ccctttttga acatttgcaa cacgcgccgc atgatagcct gtcggcatga
     5041 catctgcaag agtcaaaagg gacttgagca tcccttctgt atagtcagaa ggttgaccag
     5101 ggattttaac cagcgcccag tttgcatagt ggaagcgaat atattctgcc tgaaaatcac
     5161 cccccaaatt attgccaata tgattgtcgc aagaaccgtc aaatccagca agacaggcat
     5221 cacactcacc acatccatgt gtaaaaggga caatcacaaa atcacctggt ttcaccgtcg
     5281 taatggcttc cccagcttct tcaacaatcc caatcgcttc gtgtccacta tttttgtgtc
     5341 cagctttcgt ttctggatta cggtacctcc ataaatctga accacaaacg cacgcacgaa
     5401 ccacacgaat aatcacatca tccgcttcta ttatttgcgg acgttcaatg ctagcaagtc
     5461 caacctgacc tgcctttgta tatactgctg atttcattta aaattttcct tccttataaa
     5521 gtttaatttt gagatttaaa cgatttaaag ctgtctgtag ctgagacaag cgctcctcta
     5581 atttttgctt ttcctcttct aaaataccaa gcctctcctc tctcgtttca tctccctttt
     5641 ggtagagcga catatagtca actaaactat ctacagagac acccgccgaa cgaaaacact
     5701 taataaattc cagcgcttcg atatcctgat cttgaaaatc acgaatccca gtagcagtac
     5761 gagtaatcgg tggcacaaga ccaacccgtt cataataccg aatcgtatcc gctgaaattc
     5821 ccaacaaatc actggcagat ttaatattca cacgctaacc tcctctcatt tcattcactg
     5881 aattcacgct attctaactc acttacctta ttatataact ttgagttgac tccaagtcaa
     5941 gcattttgta tctaatcgaa aaatttttat cattaacaat catatatctg gagtttatac
     6001 aatgctcttc tcttaccaat ttttgaaata tttaaataat aaagaaatta ctctattcca
     6061 aataagatag tacccaagta tttttcactt ttttctaatg ttgatgctca taactccgtt
     6121 ccaagtcaca cacctttcgc ttatgggttt ggtgagtttc taggtcagca tcaatttcaa
     6181 tggtaatatt ttgaaaacca caatctttta ggaaaattcg aatagactct ttacaagttt
     6241 ccatatgttc catttctttt agacaaacat gtacaatggc atttttttcc aaagcatcca
     6301 tagtccagag attaagctga ttaaggctgg ccacattgtc caatcgctcc aggccactct
     6361 ttacttgctt gatatcaaga ccttctggca cagcatccaa gaaaatcttg agtgtagacc
     6421 aaaaacgtgg aagggctttt gaaagaataa agaaagaaat gacaagggac aaaagaggat
     6481 ctaggatata ccagtccgta aatcgaagaa caatcgccat caggataaca gctacccacc
     6541 ctagcgtatc ttccagaaaa tgcaaactca gaatagactc attctttgtc tttcccttac
     6601 caaccaccag actcgctaac agattgatag taatcgcaat aattcctaac cagagaatcc
     6661 cctcatcatt gactggttgc ggatgcaaaa tcttcgtgac attttccaaa atgactagaa
     6721 cagagcccgt tacgagaatc acagctgtta ccaaggctcc tagcaggcta aaccgcttat
     6781 agcccaaggt gtactgattg tcttcttcac gattggagat tgtttctaga aaagctgata
     6841 ttccaattgc aatcgcatct cccaagtcat gcacagagtc agcaagaaca gcgctagaac
     6901 caaatactcc acctgcaata aactcaacaa tggcataagt caaatttaag aaaaaagcca
     6961 cccaaacagc atattttgcc ttcatatttc tcattccttt gttataatag atttatgaac
     7021 accttgttca ttatcattat ccactaaaac tcaaagaaag gatagaagca aaacgtcagc
     7081 tttattcagt tctgaacaat ttgccttaag tgtccatatg actaacattg accgccgtat
     7141 cagcaaaacc aaaaaagcca tctatcaagc ttttatacaa cttttgaatg ctaagggcta
     7201 cgaggccact actgttcagg atatcattga tctcgcagat gtgggaagat ccacctttta
     7261 ctgtcactat gaaagtaagg agcttcttct ggaccagctc tgccgctacc tcttccatca
     7321 tctttttgaa agagagcaag ccatttcaac cgaggactac ctcgcccacc tctttctcca
     7381 ttttcataaa aaccaagacc atatcaccag tctgctattt tccaaaaatg actacttcct
     7441 ccgtcaactc cataaagagc tagaacacca tgtctattcc gtgctagctg ataatttaaa
     7501 agaagcccac cctaatatgc ctacttctta cctccaacac ttggtcgtgt ccaactttat
     7561 cgagacattg acctggtggc tcaaaaaagg tcaagatttc acaggccagg aagttgtcca
     7621 attttatcta gaccttctca ttcctaaaaa ttgaatatag agtaaagctc agttgtctta
     7681 tttctaggtt actgagtttt ttatcttttc aacaacaaaa gaggacccgc cgatcctctt
     7741 tttcatacta taaatccttg attatcaact atatctgttt taatcgaaat ctcaaaacag
     7801 cactttcaaa catcttttcc tagttaagta aatcagtatt ctgcttagct gccttgctcc
     7861 attgatacca accaactaga ctgttaatga gataaattag atatttccct tgaatttgca
     7921 ggctttctcc ccaccagaga tagattgaaa aaacattggt agccgcccag aatatccact
     7981 gttcacggta aacagctgtc atgaggattt gccctacccc attggttgca tctgtgattg
     8041 aatcacgata gggacgattg gcaccaatag actgataaat gaagccaaag gccaaccacc
     8101 aaagcacact aatggaaaga tactttgtcc agcccttgtc gtccagttta cgcgcgacaa
     8161 actcctgctt ttccttctta aactgtgcct gataaatcca aactagaagt ccaattggct
     8221 gcatgactgt gaagtaaagt gtcgtcagca cctcaccata aaagcctttc tgtagggcca
     8281 aaataaggta aataacagag ttaatcaagc caaaaagata attacttgct cgaccttccg
     8341 atacaaagat tacacagata atccctgtca agctacaaat catcccaatc cagtcaacaa
     8401 tacgatgttc gtaaaccaac tccagccaga gaggaaaact tcctaaaacc agcaaataaa
     8461 tccactgggc aaaactacga tgggcaaaga ggtcatccca gatagccttc atagttcctg
     8521 aaaatcctaa atcagccata gccgcaacca tacgacggta accacctgac atttcaccta
     8581 gggttgtttt gatattttca attttctttt gcaaataagt atgcatcatt tctccttttg
     8641 tttttaaaga gccgtgtctg gatagacttt cggacgcaac gctctattag ataatgaact
     8701 gcctatacac aagatttcta accttagtcg acatgagcta aaacctctta tttgttaagt
     8761 agttcacaaa atattataca cctattttat gaatagtcaa ctgtctttac agtaaaattt
     8821 tagaaaatca tgaaaatttt ctctttcttt ccattttaag tgacattcag tcattctcac
     8881 atcaaaaaag cccagacgaa attgtctgag cattctttta tctagtcgtt taaggaagtt
     8941 gagttcagta tgtttaaagt ctctgtccca tcatttcttc aacaaacctt gttcttggag
     9001 aaactccttg gctacttgct ttgctgactt gccttcaaca ccgacttggt agttgagctg
     9061 gctcatctgg ctttctgtaa tcttaccagc caatgtatta agaactcttt ccaactctgg
     9121 gtgtttcttg agaagagctt ctttcatgag tggagcccct tgataaggtg ggaagagttg
     9181 cttgtcatct tccaagacct gtaaatcata acgctccaat tccgcatcag tcgaataggc
     9241 atccgtgatt tgaatatccc ctgactgaat tgcctgataa cgaagagctg gctgcatagt
     9301 cgccacattg agattgagac catacattga ttgcaagccc ttatttccat cttcacggtc
     9361 gttaaactca agcgtaaagc ctgccttcag ctgcccttcc acttttttca aatccgaaat
     9421 ggtcttcaag ccatattctt gagcaatctt tttcggaaca gctacagcat aggtattttg
     9481 ataagacata ggtttgagat aggctagatg atcctgcttg gcaatgccat cacgcgccac
     9541 ttgataaacc tgctctggct catgactcac cttgggtgat ggttgaagca aactttcagt
     9601 caccgtaccg gtaaattcag gataaatgtc aatatcacct tttttcagag cttcataaag
     9661 gaaacttgtc gtcccaaaat tcggtttaac agtcgcagtc atgctggtat tttcttcaat
     9721 cagcaattta tacatattag ccaaaatttc tggttctggg cctattttcc cagcaataat
     9781 caagttttcc ttctcttttt gagccaaaag ggctggacta taagacagcc ccagtaataa
     9841 agccaccaag gcaaaaccag agaaaatcgt ccgcaatttc gctttttcca ttacttttag
     9901 taggaagtta aaggcaatag ctagcactgc agaagaaagg gccccaatca aaatcaaact
     9961 ggcattatta cggtcaattc ccaacaggat aaaggaaccc agtccccctg ccccaatcaa
    10021 ggtcgccaag gtagccgtac cgataatcaa gaccgctgcc gtccgaattc cagacatgat
    10081 aacaggcatg gcaagtggaa tttcaaactt cttgagacgc tcccatctgg tcatcccaaa
    10141 ggcaatccca gcctcttgaa gactcggatc aattcccttc agcccagtaa tagtattttg
    10201 taaaatcgga aaaatcgcat aaatcactag agctgtcaat gccggtaaag tcccaattcc
    10261 catcaaggga ataaagagtc ccaacaaggc cagagacggg atggtctgga aaatccctgc
    10321 aatctgtaag acccaatccg ccaacttctc atgatagcga agataaacag ccaagggaat
    10381 cgcaagcaaa atagctagta acaaggtcaa aagtgacaac tgcaaatgtt gagatagggc
    10441 tgtcaaccaa tcaccaaaac gatcctgaaa agttgcaatt aaattagtca tgaatactac
    10501 ctccaaacaa gtctgcaaca aagtctgttg caggagcttt taaaatagtc tcaggattcg
    10561 acacctgacg aatctctcca tcctgcaaaa cagcaatacg atctgccaac tttaaggctt
    10621 catccgtatc atgagtcaca aaaatcgttg tcatcccaaa ctctttatgt aattcttttg
    10681 tcagaacctg caactgcttg cgagaaatag catccaaggc tgaaaagggt tcatccatga
    10741 ggagaatctt gggctgacca atcatagctc ggacaatacc gacccgttgc tgttctccac
    10801 cagataattc actaggtaag cgatgcccat actcggctac tggtaaacca accttagcca
    10861 aaagctcttc tgttttcttc gtaatttctt ccttgctcca ccccttcatt tcaggaatga
    10921 gagcaatatt ttccgcaact gttagatttg gaaaaagagc aatagcctgt aaaacataac
    10981 cagtagaaag acgaagttca cgctcatcat agtctttgat gcgcttccca tccatataaa
    11041 tatttccatc agttggttcc aaaagacggt taatcatctt gagcatggtc gtcttacctg
    11101 acccagaagg ccctactaaa accataaatt ccccatcctc aatctgtaag ttgacatctc
    11161 tcaagacatc cttttctgtg tagcgcagtg ctacattttt gtattcaatc attctttgtc
    11221 ctcaatttaa aacttccctc gattggtcaa gtcttctacc ttaggcatga cttccttatt
    11281 atcccaatgc tccacaattt tcccgttctt taaacggaag atatcgtact tggcataagc
    11341 aacgccatca atctgagtct gaccatagct aaccacatag tttccttgtc ctaagagttg
    11401 gaaaacaaag tcaaaagtga cactatattc agccacatag tttttataag cagcacttcc
    11461 ttgtccaatc tcatgattat gctgaatcaa atcgtctgcc acataatcac tccactgctc
    11521 tagctcccca ttttggaaaa tttctgtcaa gaaacggcga accagctttt tattttctgc
    11581 tttcttatcc aaatccttga tttcaaaatc tccaaaaatt tgatctagtt ggtcattttc
    11641 aggtgttcga tagtagtcaa tgacatccca atgctcaaca atacgaccat tctcatccgc
    11701 acggaaagta tccgtcgtca cccattgagc ttctccacca ttcagatatt gatgaacatg
    11761 aacaaagacc agattgccat cctcaatggt gcggacaatc ttaatctgac gctctggata
    11821 acgctcaaag aaatctgcaa agaaggctgc aaatccttct ttcccgtcag gaacacctgt
    11881 cgaatgttgg atataggtat cccctacaga ctgggcttga gcctcagcaa ctcgtccgtc
    11941 ttgaatggca tggatgtata ggttgtgagc atttttcact tgttgtgaca tattctaaac
    12001 ctcatttccc ttctctttca gattcgccaa aattctttct tgaaaacctt caaattggtg
    12061 aatttcttcc tctgaaaatc ctttgtaaaa gatagtatcc aatttctgac tgacacgatg
    12121 ccccacttct ttctgggact tgcctaactc cgttaaaact aaatacttct tacgcttgtc
    12181 ttttccacac ggactaacaa ttacaagctt ttgttcctct agctttttta tcatagtcgt
    12241 cagcgtatta ttcgcaagtc cagtcgcaag cgcgatatct gtcgcagttg cgcagccagt
    12301 ttcactattc cataaaaccg ctaaaatctt gccctgttca cccctataaa gagcctcagg
    12361 atcttgactc agtaactttt gaaaaatccg cccattcaac aaacgaatat gatgggctag
    12421 caaatgacca tctttcataa cacctccaat ttatttcgat atcgaaatga ataaaacaat
    12481 tgtaacactc atcgttctaa ctgtcaacta tttcgattta gaaataattt ttgataatta
    12541 tccacatcac catactccgg ctcaactaac ttttaacgag agtttctaaa ctccttcgtc
    12601 ctccagtcta caaaagcctt ccattcgtac tatcctatat tttatgaggg gacacatttt
    12661 tcctatcaga ccatttattt taaagataga agtaaatcat aattgcttcc atctgttctt
    12721 ttatagtata ttgaagttag actagagcac tgtatcttct aaaacattga tagaaagcga
    12781 tttgaatttc ccaatcaatt tgttcgtatt tatagcattt cgaaactgga ataggacacc
    12841 atgactgcta aaagatttct ataaattcat ttaatttcct caatcaattt gttcatatct
    12901 tatttcattc cgctataatt tcaccttacc ctatcttttt cgtagcaccc ttcaaacagc
    12961 ctatccccta ccgtttgacg attcctcact tcgctccact tccattacag aagtttcttc
    13021 actactatgg gctcggctga cttctcatga ttccttgtta ctactatttg aacgctcacg
    13081 agatagatct tacaaaaaat gctttgatcc acaatggaat caaagcattt taaagagttc
    13141 ctcatacata agcgcagaag tcgcagttcc tctgtacttg gcttcttctc ttttgataaa
    13201 gcgagccaag ttgagcaact caggtgctgg atgtttggga tttaggagca attcacgatt
    13261 gaccaggcct gagagacgaa ctgcctgcaa ttgctcattt gtagtaggca gttttttagc
    13321 agtctctagg agagcagcaa ctaaatcttc actcaaatca tgtcgagcat gattgtaaag
    13381 atcttttata aggctttcta ggtttggttc tgccatccct accacctccc ttatggttta
    13441 ataatgttta atcaaatcaa ccgttgaacg atccaatttc ttcaccaagg cttgtaagaa
    13501 agcttgcgct tctaggaagt catccattgc atagagggtt tggtgagaat ggatataacg
    13561 agcgcagaca ccgatagttg ttgatgggac accaccattt ttcagatgag ctgcacctgc
    13621 atctgttccg cctttaccac agtagtattg gtacttgata ccagcttctt cagccgttgt
    13681 caaaagaaaa tccttcatcc ctgggagaag caagtgacct ggatcataga aacgaatcaa
    13741 ggttccatct ccaatcttgc cttgaccacc gtagacatca cctgctggtg agcaatcaac
    13801 tgcgaggaag acttctgggt caaacttggt tgtagaggta tgagcgccac gcagaccaac
    13861 ttcttcttgg acgttagaac ccagatagag ttcattgccg agtttttgac ctgataaagc
    13921 ttcagctagc tcgcttacca tgaggacacc gtagcggtta tcccaagctt ttgagatgat
    13981 atttttttca ttggctgtca aaattgcaga actatctggt acgatggtat caccaggacg
    14041 gatgccaaaa ctttctgcct catccttgtc cgcaaaacca ccatcaaaaa cgatatcggc
    14101 aatggctggc atggttggtc ccccctttcc acgagtcaaa tgcggaggaa cagaacctga
    14161 aatcacagga atttcatgac catcacgagt caagagtttg aaacgttggc tgctaactac
    14221 catggggttc cagccaccga tttctacgac acggaaggta ccatctggct tgatttcgct
    14281 gaccataaaa ccaacttcgt ccatatgaga agcgaccaag acgcgcggtg catccacagc
    14341 ttctgaatgt ttgataccaa aaataccacc caagccatct gtcaccactt catccacatg
    14401 cggtgtcaac ttttcacgaa gataagcacg gacaggcgct tcatgacctg agactgcagc
    14461 aagttctgtt acttctttaa tttttgaaaa taatgttgtc atttcagttc cttctttctt
    14521 tcatccattt taccactttt tataggagaa ggatagtggg aaggtggatt tctaagttag
    14581 tatcttagtc ctgctctatc ttagaaaagg atagtattct cttgcatgta gtgcaaaatc
    14641 tagtaaacat tccaaaatta actcgaatat ttatttccaa acaaaaaaac aatacaccat
    14701 caaagttgtt tgaatttttc atgaaattta cagaaaatag ttgacttccc tttcttcttt
    14761 ctttaaatat atagttggtt gagtttggaa tagtacgctg tagctgctga aacatttcta
    14821 gaaatt
//
LOCUS       DAVLVP010000042        14826 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.042, whole genome shotgun sequence.
ACCESSION   DAVLVP010000042 DAVLVP010000000
VERSION     DAVLVP010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14826
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.042"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..1515)
                     /gene="pfbA"
                     /locus_tag="VYN69_001229"
     CDS             complement(<1..1515)
                     /gene="pfbA"
                     /locus_tag="VYN69_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multi-ligand-binding adhesin PfbA"
                     /protein_id="HEV1533503.1"
                     /translation="MKYFVPNEVFSIRKLKVGTCSVLLAISILGSQGILSDEVVTSSS
                     PMATKESSNAITNDLDNSPTVNQNRSAEMIASNSTTNGLDNSLSVNSISSNGTIRSNS
                     QLDNRTVESTVTSTNENKSYKEDVISDRIIKKEFEDTALSVKDYGAVGDGIHDDRQAI
                     QDAIDAAAQGLGGGNVYFPEGTYLVKEIVFLKSHTHLELNEKATILNGINIKNHPSIV
                     FMTGLFTDDGAQVEWGPTEDISYSGGTIDMNGALNEEGTKAKNLPLINSSGAFAIGNS
                     NNVTIKNVTFKDSYQGHAIQIAGSKNVLVDNSRFLGQALPKTMKDGQIISKESIQIEP
                     LTRKGFPYALNDDGKKSENVTIQNSYFGKSDKSGELVTAIGTHYQTLSTQNPSNIKIL
                     NNHFDNMMYAGVRFTGFTDVLIKGNRFDKKVKGESVHYRESGAALVNAYSYKNTKDLL
                     DLNKQVVIAENIFNIADPKTKAIRVAKDSAEYLGKVSDITVTKNVINNNSKETEQ"
     gene            complement(2679..3905)
                     /locus_tag="VYN69_001230"
     CDS             complement(2679..3905)
                     /locus_tag="VYN69_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001134527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HEV1533504.1"
                     /translation="MPNYNIPFSPPDITEAEIAEVADTLRSGWITTGPKTKELERRLS
                     LYTQTPKTVCLNSATAALELILRVLEVGPGDEIIVPAMTYTASCSVITHVGATPVMVD
                     IQADTFEMDYDLLEQAITEKTKVIIPVELAGIVCDYDRLFQVVEKKRDFFTASSKWQK
                     AFNRIVIVSDSAHALGSTYKGQPSGSIADFTSFSFHAVKNFTTAEGGSATWKANPVID
                     DEEMYKEFQILSLHGQTKDALAKMQLGSWEYDIVTPAYKCNMTDIMASLGLVQLDRYP
                     SLLQRRKDIVDRYDSGFAGSRIHPLAHKTETVESSRHLYITRVEGASLEERNLIIQEL
                     AKAGIASNVHYKPLPLLTAYKNLGFDMTNYPKAYAFFENEITLPLHTKLSDEEVDYII
                     ETFKTVSEKVLTLSKK"
     gene            complement(3948..4640)
                     /locus_tag="VYN69_001231"
     CDS             complement(3948..4640)
                     /locus_tag="VYN69_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000922225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1533505.1"
                     /translation="MLKWEDLPVEMKSSEVESYYQLVSKRKGSLIFKRCLDWVLALVL
                     LVLTSPIFLILSIWIKLDSKGPVIYKQERVTQYNRRFKIWKFRTMVTDADKKGSLVTS
                     ANDSRITKVGNFIRRVRLDELPQLVNVLKGEMSFVGTRPEVPRYTEQYSPEMMATLLL
                     QAGITSPASINYKDEDTIISQMTKKGLSVDQAYVEHVLPEKMRYNLAYLREFSFFGDI
                     KIMFQTVFEVLK"
     gene            complement(4813..6564)
                     /locus_tag="VYN69_001232"
     CDS             complement(4813..6564)
                     /locus_tag="VYN69_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001180175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533506.1"
                     /translation="MQNKQEQWTVLKRLMSYLKPYRLLTFLALSFLLATTVIKSVIPL
                     VASHFIDQYLSNLNQLAVTVLLVYYGLYILQTVVQYVGNLLFARVSYSIVRDIRRDAF
                     ANMEKLGMSYFDKTPAGSIVSRLTNDTETISDMFSGILSSFISAVFIFLTTLYTMLVL
                     DFRLTALVLLFLPLIFLLVNLYRKKSVKIIEKTRSLLSDINSKLAENIEGIRIIQAFN
                     QEKRLQAEFDEINQEHLVYANRSVALDALFLRPAMSLLKLLGYAVLMAYFGYRGLSIG
                     ITAGTMYAFIQYINRLFDPLIEVTQNFSTLQTSMVSAGRVFALIDERTYEPLQENGQA
                     KVKEGNIRFEHVCFSYDGKHPILDDISFSVNKGETIAFVGHTGSGKSSIINVLMRFYE
                     FQSGRVLLDDVDIRDFSQEELRKNIGLVLQEPFLYHGTIKSNIAMYQEISDEQVQVAA
                     AFVDADSFIQELPQGYDSPVSERGSSFSTGQRQLLAFARTVASQPKILILDEATANID
                     SETESLVQASLAKMRQGRTTIAIAHRLSTIQDANCIYVLDKGRIIESGTHEELLALGG
                     TYHKMYSLQAGAMADTL"
     gene            complement(6554..8299)
                     /locus_tag="VYN69_001233"
     CDS             complement(6554..8299)
                     /locus_tag="VYN69_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533507.1"
                     /translation="MSIIQKLWWFFKLEKRRYLVGIVALVLVSVLNLIPPMVMGRVID
                     AITSGQLTQQDLLLSLFYLLLAAFGMYYLRYVWRMYILGTSYCLGQIMRSRLFKHFTK
                     MSSAFYQTYRTGDLMAHATNDINALTRLAGGGVMSAVDASITALVTLLTMLFSISWQM
                     TLVAILPLPFMAYATSRLGRKTHKAFGESQAAFSELNNKVQESVSGIKVTKSFGYQAD
                     ELKSFQAVNELTFQKNLQTMKYDSLFDPMVLLFVGSSYVLTLLVGSLMVQEGQITVGN
                     LVTFISYLDMLVWPLMAIGFLFNTTQRGKVSYQRIENLLSQESPVQDPEFPLDGIENG
                     RLEYAIDSFAFENEETLTDIHFSLAKGQTLGLVGQTGSGKTSLIKLLLREYDVDKGAI
                     YLNGHDIRDYRLTDLRSLMGYVPQDQFLFATSILDNIRFGNPNLPLSAVEEATKLARV
                     YQDIVDMPQGFDTLIGEKGVSLSGGQKQRLAMSRAMILDPDILILDDSLSAVDAKTEY
                     AIIDNLKETRKDKTTIITAHRLSAVVHADLILVLQNGQIIERGRHEDLLALDDWYAQT
                     YQSQQLEMKGEEDAE"
     gene            complement(8505..8882)
                     /locus_tag="VYN69_001234"
     CDS             complement(8505..8882)
                     /locus_tag="VYN69_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEV1533508.1"
                     /translation="MKRLIYGKPTLLIKNGNIDPEACRSVGLSASDVSLKLRSQGIFQ
                     MKQVKRAVQEQNGQLIVVQMGDENPKYPVVTDGVIQVDVLESIGRSEEWLLDNLSKQG
                     HDNVANIFIAEYDKGAVTVVTYE"
     gene            complement(8954..9411)
                     /locus_tag="VYN69_001235"
                     /pseudo
     CDS             complement(8954..9411)
                     /locus_tag="VYN69_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730499.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3290 family protein"
     gene            9544..10371
                     /gene="xth"
                     /locus_tag="VYN69_001236"
     CDS             9544..10371
                     /gene="xth"
                     /locus_tag="VYN69_001236"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006596696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEV1533509.1"
                     /translation="MKLISWNIDSLNAALTSDSARAKLSQEVLQTLVAENADIIAIQE
                     TKLSAKGPTKKHVEILEELFPGYENTWRSSQEPARKGYAGTMFLYKKELTPTISFPEI
                     GAPSTMDLEGRIITLEFDAFFVTQVYTPNAGDGLKRLEERQVWDAKYAEYLAELDKEK
                     PVLATGDYNVAHNEIDLANPASNRRSPGFTDEERAGFTNLLATGFTDTFRHVHGDVPE
                     RYTWWAQRSKTSKINNTGWRIDYWLTSNRIADKVTKSDMIDSGARQDHTPIVLEIDL"
     gene            10383..10526
                     /locus_tag="VYN69_001237"
     CDS             10383..10526
                     /locus_tag="VYN69_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEV1533510.1"
                     /translation="MDYNAVIPEFLVSNIEQSRSFYCGLLGFRIEYQRPEENFLFLLK
                     SVN"
     gene            10637..10765
                     /locus_tag="VYN69_001238"
     CDS             10637..10765
                     /locus_tag="VYN69_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HEV1533511.1"
                     /translation="MKANYPIYRPLTKRKFRVSDPYIYPHKFAVLDPNGYFLRFSE"
     regulatory      11148..11243
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            11643..12224
                     /locus_tag="VYN69_001239"
     CDS             11643..12224
                     /locus_tag="VYN69_001239"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000770427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEV1533512.1"
                     /translation="MKLLEDRILKDGHILGDNILKVDSFLTHQVDFSLMREIGKVFAE
                     KFAAAGITKVVTIEASGIAPAVFTAEALNVPMIFAKKAKNITMNEGILTAQVYSFTKQ
                     VTSTVSIAGKFLSPEDKVLIIDDFLANGQAAKGLIQIIEQAGATVQAIGIVIEKSFQD
                     GRDLLEKAGYPVLSLARLDRFENGQVVFKEADL"
     gene            12224..13486
                     /locus_tag="VYN69_001240"
     CDS             12224..13486
                     /locus_tag="VYN69_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001194492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEV1533513.1"
                     /translation="MQTQEKHSQAAVLGLQHLLAMYSGSILVPIMIATALGYSAEQLT
                     YLISTDIFMCGVATFLQLQLNKYFGIGLPVVLGVAFQSVAPLIMIGQSHGSGAMFGAL
                     IASGIYVVLVSGIFSKVANLFPSIVTGSVITTIGLTLIPVAIGNMGNNVPEPTGQSLL
                     LSAITVLIILLINIFTKGFIKSISILIGLVVGTAIAATMGLVDFSPVAVAPLVHVPTP
                     LYFGIPTFEISSIVMMCIIATVSMVESTGVYLALSDITKDPIDSTRLRNGYRAEGLAV
                     LLGGIFNTFPYTGFSQNVGLVKLSGIKTRLPIYYAAGFLVLLGLLPKFGALAQIIPSP
                     VLGGAMLVMFGFVSIQGMQILARVDFANNEHNFLIAAVSIAAGVGLNNSNLFVSMPTA
                     FQMFFSNGIVVASLLAIVLNAVLNHKKK"
     gene            complement(13537..13998)
                     /locus_tag="VYN69_001241"
     CDS             complement(13537..13998)
                     /locus_tag="VYN69_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000850937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DpnD"
                     /protein_id="HEV1533514.1"
                     /translation="MKTKQLVASEEVYDFLKVIWPDYETESRYDNLSLIVCTLSDPDC
                     VRWLSENMKFGDEKQLALMKEKYGWEVGDKLPEWLHSSYHRLLLIGELLESNLKLKKY
                     TVEITETLSRLVSIEAENPDEAERLVREKYKSCEIVLDADDFQDYDTSIYE"
     gene            complement(13995..14759)
                     /locus_tag="VYN69_001242"
                     /pseudo
     CDS             complement(13995..14759)
                     /locus_tag="VYN69_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418959.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
BASE COUNT         4386 a         3172 c         2795 g         4473 t
ORIGIN      
        1 ttgttctgtt tccttagaat tattattaat tacatttttt gttacagtaa tatctgatac
       61 ttttcctaaa tattctgcac tatcttttgc aactcgtatc gcttttgttt taggatcggc
      121 aatattaaat atattttcgg cgataaccac ctgtttattt aaatctaata ggtctttagt
      181 gtttttatag ctataagcat ttactaaagc tgctccgctt tctcgataat gtacactctc
      241 tcctttaact ttcttatcaa agcgatttcc tttgattaat acatcagtga atcctgtaaa
      301 acgtacacct gcatacatca tgttatcaaa atgattattt aaaattttaa tattagaggg
      361 gttctgtgtc gacaatgttt gatagtgtgt gccaattgct gttactaatt ccccagattt
      421 atcacttttg ccaaaatagg aattttgaat agtcacattt tcagattttt tcccatcatc
      481 attcaaggca taaggaaaac cttttctagt taatggttca atctgaatgc tctccttact
      541 tatgatttgc ccatccttca tcgttttggg taaggcttgc ccaagaaaac gagaattatc
      601 aactaataca tttttcgaac ctgcaatttg aatagcatgc ccttgataac tatccttgaa
      661 tgttacattt tttatagtta cgttatttga attcccaata gcaaatgcac ctgaagaatt
      721 tataagtggt agattttttg ctttagttcc ttcttcattc aaagcaccgt tcatatcaat
      781 cgtaccacca gaataactaa tatcttctgt tgggccccat tctacttgcg caccatcatc
      841 cgtaaataaa cctgtcataa aaacaatgga agggtgattc ttaatattta taccatttag
      901 aattgtagct ttctcattca attctaagtg tgtatgactt tttaaaaaaa caatttcttt
      961 tactaaataa gttccttcag gaaaatatac atttcctcca cctagccctt gagctgcagc
     1021 atctattgca tcttgaattg cttgtcgatc atcatgaatc ccatcaccta ccgcaccata
     1081 atcttttaca cttaaagcag tatcttcaaa ttcttttttg ataattctgt cacttataac
     1141 atcttcctta taactcttat tttcattagt agatgttact gtagattcaa ctgttctgtt
     1201 gtctaattgt gaattggaac gaatagtacc attagagctg atactattaa cacttaacga
     1261 attatctaaa ccattagtgg ttgaattaga ggcaatcatt tcagcagaac gattctgatt
     1321 aacagttggt gaattatcta aatcattagt aattgcatta gaagactctt ttgtagccat
     1381 cggtgaagaa ctagtaacaa cttcatccga taaaatacct tggcttccca aaattgaaat
     1441 tgccaatagt accgagcaag tccccacctt taatttacga atactgaata cctcattagg
     1501 aacaaaatat ttcatcagaa cctccaattt ttttactatt tttaacaatt gattattact
     1561 tttcttaaaa tagatagaat gacaccgctg tcattatctt tatcatatat tttccaaatt
     1621 gaattgtcaa ggaataacac aagaaaatat taaaatttat actcttcgga aatctcttca
     1681 aactaggtca gtgccgcctt gccctaagtc taggttactg acttggtcag ttctatagta
     1741 gactgaaact ataatagtac acctctactt ctaaaacatt gttagaaatc gatttgacta
     1801 tcccgatcga tttgtcctat tcttatttcc ttttactata tctacaacct caaagcaggg
     1861 ttttgagcta tctatggcca acttcctagt ttgctcttta gccttccatt caaaagtgat
     1921 gctatctttc ttagcttgaa tataaaatat cccatttact atatttaaat ttattgatat
     1981 gttgtaaatt ataaatgaaa gaacaaatac cttgtcatac aagcattcgc cagggatatt
     2041 taccttaata tcgctcatat ggcggattga aactaaaaca ctacaatata tttcttaaat
     2101 agaccttcta taaaagctag atttaacctc gataaaaata gatgcagtgc ttctaaaata
     2161 ctggaatagg gcatataggt aatcgtagac aagtgtttta tagattgtct cgcaggagat
     2221 ctaatgatgt atagcaagca tcactaaaca tatgggatat tcgcattatt ataatatcaa
     2281 catgctataa attctatcat gtaaaagaat gactagaaca tgaaagacag gcacaaaact
     2341 ttgaacaata ttctgaaaac aaaaaaaaga cgcgagtttc cacaatctca ctcatggaaa
     2401 ctcacgtctt tcaaaaaata gggtgaattg aaaactagaa caagacacta gattttctaa
     2461 atgatgtcta gtaaggattc taaatgctac accaaattgc tcacatctta tttcaaacca
     2521 cgggactccc acaagaaagg aaaatcctat aaaaattctc tctctgagga gaaaggagcc
     2581 tcaactacag gtgtaaacta ttatcaattg aaaacaacag tttttacact caattagact
     2641 ttttaaggac aggatcactt tcgcttgact gtagtttgtc atttttttga taaagttagc
     2701 actttttcag aaactgtttt gaaagtctca atgatatagt ctacttcttc atcgcttaat
     2761 ttagtatgaa gagggagggt aatttcattc tcaaagaagg cataggcctt aggatagttc
     2821 gtcatatcaa atccaagatt cttataggct gtcaagagag gaagcggttt gtagtgaaca
     2881 ttacttgcaa ttcctgcttt agccaattct tggatgatga ggttgcgttc ttctaggctt
     2941 gctccttcta cacgggtgat gtagaggtgg cgtgaagatt cgacagtttc agtcttgtgt
     3001 gccaaaggat ggatgcgaga acctgcaaaa ccactatcat agcggtccac aatgtcctta
     3061 cggcgttgca acaaacttgg atagcggtcc aattgtacca aaccaagtga agccatgata
     3121 tcggtcatgt tgcacttata ggctggtgta acgatatcgt attcccatga ccccagttgc
     3181 atcttggcaa gagcatcctt agtttgcccg tgaagggaaa ggatttggaa ttccttgtac
     3241 atctcttcgt catcaatcac tggattggct ttccaagtcg cacttccacc ttctgccgtt
     3301 gtaaagttct taacggcatg gaatgagaag gaagtaaagt cagcgataga accagaaggt
     3361 tgtcctttat aagtagatcc caaagcgtgg gcactatcag agacaatgac aatacggtta
     3421 aaggcctttt gccacttgct tgaagcggta aagaagtcac gttttttctc cacgacttgg
     3481 aacaaacggt cataatcgca aacaatccct gcgagctcta ctgggataat caccttagtt
     3541 ttctcagtga tagcttgctc aagcaggtca tagtccatct caaacgtatc tgcttggata
     3601 tccaccatga caggggttgc tcccacgtgc gtaatgacac tacatgaagc cgtataggtc
     3661 atggctggaa cgatgatttc atcaccaggt cccacttcca aaacgcgtaa aatcaactcc
     3721 agagcagctg tcgcagagtt gagacaaaca gtcttaggtg tctgtgtgta aagagacaag
     3781 cggcgctcca gttcttttgt tttaggacct gttgtgatcc aaccagaacg cagggtatcc
     3841 gctacttcag caatttctgc ttctgtgata tcaggcggtg aaaatggaat attgtaattt
     3901 ggcattgatt tgctcctttt atctgtactc attttcttat gactacttta ttttagtacc
     3961 tcaaacacgg tttgaaacat gattttgatg tccccaaaga aactaaactc tcggagatag
     4021 gcgaggttat agcgcatctt ttcaggaaga acatgctcca cataggcctg atcaactgac
     4081 agacctttct tcgtcatttg actgatgatg gtgtcctcat ccttgtagtt gatgctggct
     4141 ggagaggtaa tccctgcttg caagagcaag gttgccatca tttcagggct atactgctct
     4201 gtataacgtg gcacttcagg tcgtgtaccg acaaaggaca tctcaccttt aaggacattg
     4261 accaactgag gcagttcgtc caaacggaca cgtcggatga aatttccaac cttggtaata
     4321 cggctatcgt tagcagaagt caccagactt ccttttttat ccgcatccgt caccatggta
     4381 cggaacttcc aaatcttgaa ccgacggttg tactgggtca cacgctcttg cttgtaaatc
     4441 actggcccct tgctatccaa cttgatccaa atgctcaaga tgagaaagat gggagaggtc
     4501 agaaccagta agaccaaggc caaaacccag tccaagcaac gcttgaaaat cagcgaaccc
     4561 ttccttttag agacaagctg gtagtaagac tcaacctcgc ttgatttcat ttccacaggc
     4621 aagtcttccc atttcagcat gctgttctcc tttgttttta ttatactatt tgtcaacatt
     4681 tttcattata ccacaaaatg gaaaaggcgg tgaaagaaat ctgtaatttg aaggatttcc
     4741 ttctagtact caatgaaaat caaagtacag ctttgagatt gcagataaag ctgacatggt
     4801 ttaaagagat tttcaaagag tatcggccat ggcccctgcc tgcaaactat acatcttgtg
     4861 ataggttcct cccagagcca agagttcctc atgggttcca ctctcgataa tgcgtccctt
     4921 atccaagaca tagatgcagt tggcgtcttg aatagtagaa aggcggtgag cgatagcaat
     4981 agttgttcgg ccctgtctca tcttcgccag agaagcttga accaagcttt ctgtttcaga
     5041 gtcaatattg gctgtcgctt catccaaaat caggatttta ggctggctgg cgactgttct
     5101 agcaaaggca agaagctggc gctgcccagt agagaagctc gaaccacgct cggaaacagg
     5161 ggagtcgtac ccctgaggaa gttcttgaat aaaggaatct gcatccacaa aggctgccgc
     5221 aacctgaacc tgctcatcac tgatttcttg gtacatggcg atattggact taatagttcc
     5281 atgatagagg aagggttcct gtaagaccaa accgatgttt tttctcagct cttcttgact
     5341 gaaatccctg atatccacat catccaagag aactctccct gactggaatt cataaaagcg
     5401 catgaggaca ttgataatag acgatttccc tgaacctgta tgacctacaa aggcaatggt
     5461 ttcaccctta ttaacagaga aagaaatgtc atccagaatc ggatgtttac cgtcatacga
     5521 gaaacacaca tgttcaaaac ggatattgcc ttctttgact ttagcctgcc cattttcttg
     5581 aaggggctca taggtcctct catcgattaa ggcaaagaca cggcctgcag aaaccataga
     5641 cgtttgcagt gttgaaaagt tttgcgtcac ctcaatcaag gggtcaaaga ggcggttgat
     5701 gtactggata aaagcataca tggtcccggc cgttatcccg atagaaagac cacgatagcc
     5761 aaaataggcc atcaagacag cataacctag aagtttcaac aaactcatgg caggtctcaa
     5821 aaagagggca tccaaggcta cagaacggtt ggcatagacc aagtgctctt ggttgatttc
     5881 atcaaattct gcctgcaggc gcttctcttg attaaaggcc tggattattc tgattccctc
     5941 gatgttttca gccagcttgc tattgatatc tgacaagaga cttctggttt tctcaatgat
     6001 tttcactgat tttttccgat agagattgac caaaaggaaa atcaagggga gaaagagcaa
     6061 gactaatgct gtcaaacgaa aatccaatac caacatggta taaagggttg tcagaaagat
     6121 gaaaactgct gagataaagc tagataaaat ccccgaaaac atatcactaa tcgtctcggt
     6181 atcgttggtc aaacgagaga cgatagagcc tgctggcgtc ttgtcaaaat aagacatgcc
     6241 caacttttcc atattggcaa aggcatcccg acgaatatcc ctaacaatac tgtaagacac
     6301 gcgcgcaaag agaagattgc cgacatactg aactacagtt tgtaggatgt agagaccata
     6361 gtagaccagc aaaacggtaa cggctagttg gttaagattg ctgagatact ggtcgataaa
     6421 gtgggaagcc acgaggggta tgacactttt aatgaccgtc gtcgctagga gaaagctgag
     6481 tgccaaaaag gtcaggagtc tataaggctt gagataggac atcaagcgct tcaatacagt
     6541 ccattgttct tgtttattct gcatcttctt ctcccttcat ttccagctgc tgagactggt
     6601 aggtttgggc ataccagtca tccaaagcta gcaagtcttc gtgcctgcct cgttcgataa
     6661 tttgaccatt ttgcaggact aaaatcaaat ctgcatggac aactgcactc aggcgatggg
     6721 cagtgataat ggttgtcttg tcctttcgcg tctccttgag gttgtcgata atcgcatact
     6781 ctgtcttggc atctacggcg gataaggaat catccaaaat caagatatca gggtctaaaa
     6841 tcatagcccg actcatagcc aaccgttgct tttgaccacc agaaagactg actccttttt
     6901 caccaatcag cgtatcaaat ccttgaggca tgtctacaat atcttggtaa actcgggcta
     6961 gcttagtagc ttcctcgacc gctgaaaggg gcaagttagg attgccaaag cggatattgt
     7021 ctaggattga agtcgcaaag agaaactggt cctgaggaac atagcccatg agactgcgaa
     7081 ggtctgtcag acgatagtcc cgaatatcgt gaccgtttag ataaatggca cccttatcca
     7141 catcgtattc acgcaagagg agcttgatta aggacgtttt cccagagcct gtctgcccaa
     7201 ccaagcccag tgtttgccct tttgccaaac taaagtgaat atccgtcagt gtttcctcat
     7261 tttcaaaagc aaagctgtca atggcatact ccaaacgccc attttcaata ccatccagag
     7321 gaaactcagg gtcttgtaca ggagattcct gagacaaaag attttcaatc cgctggtaag
     7381 aaaccttccc tcgctgagta gtattaaaga ggaagccgat ggccataaga ggccagacca
     7441 gcatatccaa atagctgata aaggtgacta gattcccaac tgtaatctgc ccttcctgaa
     7501 ccatcaagga gccaaccaaa agcgttaaaa cataggacga accaacaaac aagagaacca
     7561 tagggtcaaa gagactatca tatttcatgg tttgcaggtt cttttggaag gttaattcat
     7621 tgactgcctg aaaagacttc aactcgtctg cctgataacc gaaagacttg gtcactttga
     7681 tacctgatac ggactcctgt accttgttat tgagttcaga aaaagcagct tgggattcgc
     7741 caaaggcctt atgagtcttt ctccctaggc gactagtcgc ataggccatg aaaggtaggg
     7801 ggagaatggc aacaagagtc atctgccatg agatgctaaa gagcatggtc aacaaagtca
     7861 ccagagccgt gatagaggca tccaccgcag acatgacacc gccacctgct aaacgagtca
     7921 aggcattgat atcattggtt gcgtgtgcca tcagatcacc cgtccgatag gtttgataaa
     7981 aggctgacga catttttgtg aaatgcttaa acaagcgaga ccgcatgatc tgtcccaagc
     8041 aataagaggt cccaaggata tacatacgcc acacatagcg caaatagtac ataccaaagg
     8101 ctgcaagtag caagtaaaat aggctaagaa ggaggtcctg ctgggttaat tgccccgatg
     8161 tgatggcatc aatgacccgc cccataacca taggaggaat gagattgagg acggaaacca
     8221 agaccagggc cacaatcccg actagataac ggcgtttttc taacttgaaa aaccaccaaa
     8281 gtttttgaat aatggacata aaatcccttt ctgattgcaa atggaaacct gaggccaata
     8341 ctcaatgaaa atcaaagagc aaactaggaa actagccgca ggctgctcaa agcactgctt
     8401 tgaggttgta gatagaactg acgaagtcag taacctacat acggcaaggc gacgttgacg
     8461 cggtttgaag agattttcga agagtacaag accccaggtt tttcttattc ataagttacg
     8521 actgtaacag cacccttgtc atattcagca ataaagatat tggctacatt gtcatgccct
     8581 tgtttactga ggttatcaag caaccactct tcgctacgac caatcgattc caagacatct
     8641 acttgaatca caccgtcagt cacaactgga tacttaggat tttcatctcc catttgcaca
     8701 acgatgagtt gcccattttg ctcttgcaca gctcgtttga cttgcttcat ctggaaaatc
     8761 ccttggctac gaagtttgag ggatacatcc gatgcagaca aaccaactga acgacaggct
     8821 tctgggtcaa tattcccatt tttgataagg agagttggct taccataaat caaacgtttc
     8881 acaaagtgaa cattgttgtt gagctatttg agagtcaaga ccagtttgat taaaatttcg
     8941 atataattaa gtgtcatttg ttcacatcca ccagttcaat tgcgtctgtc ttatacaatt
     9001 cgactttctc taacaggtac ttgtctggct cacttccgtt tagggcacga taatagcgtt
     9061 ctcccacctt gataagtgcg ccatctgtgt ttgtggaagt attaacatag acttctgact
     9121 tgtctactca caaatctttg gaaacaacct cgatgaaatg aagtgaagtt tgaaatttat
     9181 tgttagaaac ttgattgttt tgataagtcg tgatgctgac caaaagcaga gctaaataag
     9241 gccagagctg aaatcatgac caactcacga aatttggttc cttttttatc atggtatgcc
     9301 ttaaaagcaa aaaaaaactg tcacagctaa tagaacaatc ccaaagccaa ccatgatacc
     9361 attttgctga ccgatttggc tgagtacata gtcataagag tagaatttca tgtattttca
     9421 ctccctttca taaaatcatt cttaattata aacgaaagag agtaattttt caaaccatac
     9481 gttggggaaa tagatctttt tttggtataa taaaatctat aatctgaatg aaaaggtaac
     9541 tttatgaaac ttatctcatg gaatattgat tccctaaacg ctgccctaac tagtgactca
     9601 gctcgtgcca aattgtccca agaagtccta caaaccttgg tcgctgaaaa tgctgatatc
     9661 attgctatcc aagaaaccaa gctttctgcc aaaggaccta caaagaaaca cgtggaaatt
     9721 ttagaagaac tcttcccagg ctacgaaaac acgtggcgtt cttcccaaga gcctgcccgt
     9781 aaaggctatg ctggaaccat gttcctttat aagaaagaac ttacacctac tatcagcttc
     9841 ccagaaatcg gtgccccttc taccatggac ttggaaggtc gtatcatcac tctagaattt
     9901 gatgcatttt tcgtgaccca agtttacaca ccaaatgctg gtgacggtct caaacgcttg
     9961 gaagaacgcc aagtctggga tgccaaatat gctgagtatt tggctgaact agacaaagaa
    10021 aaaccagtcc ttgcgaccgg tgactacaac gtagcccaca atgaaatcga ccttgcaaat
    10081 cctgctagca accgccgttc acctggattt actgacgagg aacgtgctgg atttaccaac
    10141 ctattggcaa ctggatttac tgataccttc cgccatgttc atggcgatgt tcctgaacgc
    10201 tacacttggt gggcacaacg cagcaaaact tctaaaatca acaatacagg ctggagaatc
    10261 gactactggc tcacaagtaa ccgtatcgct gacaaggtca ctaaatctga tatgattgac
    10321 tcaggtgctc gccaagacca tacaccgatt gtcttggaaa ttgacttgta aggattttct
    10381 atatggacta caatgcggtc attcccgagt ttcttgtatc gaatatcgaa caatcccggt
    10441 ccttctactg tggtttactg ggttttagga ttgaatacca acgtccagaa gaaaactttc
    10501 tctttctctt gaagagtgtc aactaatgtt agaggaggga acaaagaatc aactagctga
    10561 actgacctac ccctttggtc gtggagtcaa tctctccttt ggcataaagg atgtttcaaa
    10621 attctattaa agagtgatga aggcaaacta tcctatttat cgtcccttga ccaaaagaaa
    10681 gtttcgtgtc agtgatccct atatctatcc tcataaattt gcagttttgg atccaaatgg
    10741 ctatttttta agatttagcg agtagaataa taaaaattgg gaaaagtctt taaagttaga
    10801 aaaaggcata ttgtcaggtg tctaaaaact tgatagtatg ccttttatga tggaaaaatc
    10861 ttctaatact cttcgaaaat ctcttcaagc cacatcaacg tcgccttacc gtaggtatat
    10921 gatactgact ctgtcaattc tatccacaac ctcaaaacag tgatttgagg ttgtggatag
    10981 cttcctagtt tgctctttga ttttcattta gtataaatgt tagcttacaa tggagttttt
    11041 gttcaccccc tcttctgttt gttcgttttt taaaccaaaa cactaacaaa atagttgatt
    11101 tataaagtta tttagtgtaa aataaaaaca ttggctcaag cctatttaaa attgaatatc
    11161 gttttacttg tttatgtcgt gaattggcac gacgtttcta caaggtgccg gaacacctaa
    11221 caataagtaa gtcagcagtg agatatggcc gtttttgcca tgcctatttg ggggacttac
    11281 ttagagatta agtaggtcct ttttgaatat ttttaatatt tcccttaatt agtgcggatg
    11341 ggaggtgaaa ttatcctgtg aataatttca ggaatccagg gaatttcatc cctaattttt
    11401 gagcccctag ctctttcatt tctaggctta ggctaaaata gttccccgaa ctattttact
    11461 ctcaaaaatc ccgtcataga caataacaag attattgtct atgataccac tacatcggga
    11521 aatatttgat aagactcgct tcgctcgtaa tagttttatc aaatatatag tattagcaaa
    11581 agggatcact atagaaacat ttaaacaaca agtaaattag cttttagact ttaggaggtc
    11641 ttatgaaatt attagaagat cgcatcctca aggatgggca tatcttgggt gataacatcc
    11701 tcaaggtaga ttccttttta acccaccaag ttgactttag cttgatgcga gagattggta
    11761 aggtttttgc ggaaaaattt gctgctgccg gcattaccaa ggtcgtaacc attgaagcgt
    11821 cgggtattgc cccagccgtt tttacagctg aagccttaaa cgttcccatg attttcgcca
    11881 aaaaagctaa gaacatcacc atgaacgaag gcatcttaac tgctcaagtc tactccttta
    11941 ccaagcaggt gaccagcacc gtttctatcg ctggaaaatt cctctcacca gaggacaagg
    12001 ttttgattat cgacgatttc cttgctaatg gccaagctgc taaaggcttg attcaaatca
    12061 tcgaacaggc cggtgccaca gtccaagcta tcggtatcgt gattgagaaa tccttccaag
    12121 atggccgtga tttgcttgaa aaagcaggct accctgtcct atcacttgct cgtttggatc
    12181 gttttgaaaa tggtcaggtc gtatttaagg aggcagatct ctagtgcaaa ctcaagaaaa
    12241 acactcgcaa gcagccgttc ttggcttgca gcacttacta gccatgtact caggatctat
    12301 cctggttccc atcatgattg cgacagccct tggctattca gctgagcagt tgacctacct
    12361 gatttctaca gatatcttca tgtgtggggt ggcaaccttc ctccaactcc aactcaacaa
    12421 atactttggg attggactcc cagtcgttct tggagttgca ttccagtcgg tcgctccctt
    12481 gattatgatt gggcaaagcc atggtagtgg cgctatgttt ggtgccctta tcgcatctgg
    12541 gatttacgtg gttcttgttt caggcatctt ctcaaaagta gccaatcttt tcccatctat
    12601 cgtaacagga tctgttatta ccacgattgg tttaaccttg atccctgtcg ctattggaaa
    12661 tatgggaaat aacgttccag agccaactgg tcaaagtctc ttgctttcag ctattactgt
    12721 tctgattatc ctcttgatca acatctttac caaaggattt atcaagtcta tctctatttt
    12781 gattggtctg gttgttggaa ctgccattgc tgctactatg ggcttggtgg acttctctcc
    12841 tgttgcggta gctccacttg tccatgtccc aactccactc tactttggga ttccaacctt
    12901 tgaaatctca tctattgtca tgatgtgtat catcgcaacg gtgtctatgg ttgagtcaac
    12961 tggtgtttat ctggccttgt ctgatatcac aaaggatcca atcgacagca cgcgccttcg
    13021 caacggatac cgcgcagaag gtttggccgt acttctcgga ggaatcttta acaccttccc
    13081 ttacacagga ttttcacaaa acgttggttt ggttaaatta tcaggtatca agactcgcct
    13141 gccaatctac tacgcagctg gtttcctggt tctccttgga ctcctaccta agtttggcgc
    13201 ccttgcccaa atcattccaa gtcctgtcct cggcggtgcc atgctggtga tgtttggttt
    13261 tgtatcaatt caagggatgc aaatcctcgc ccgtgttgac tttgctaaca atgaacacaa
    13321 cttccttatc gcagctgttt caatcgctgc aggtgtcggt ctcaacaaca gtaatctctt
    13381 tgtcagcatg ccgacagcct tccaaatgtt cttctcaaac ggaatcgtcg tagccagcct
    13441 actcgctatt gtcctcaatg ccgtattaaa tcataaaaag aaataagaaa aagaggtgcg
    13501 agcctctttt ttgtgtgaca aaataaaaac atctacctat tcatatatgc tagtgtcata
    13561 gtcctgaaaa tcatctgcat caagaacaat ttcacaactc ttatactttt ctcttacaag
    13621 tcgttcggct tcatctggat tttcagcctc tatacttact aaacgtgata aagtttctgt
    13681 aatttctact gtatactttt tcagttttag attgctttcc aataattcac ctattaacaa
    13741 taatctatga taggagctat gtagccactc tggcaattta tctcctactt cccacccata
    13801 tttttccttc atcaaagcta gttgtttttc gtcaccaaat ttcatatttt cagataacca
    13861 tctcacacaa tcgggatctg ataaggtaca gacgattaaa cttaggttat cgtaacggct
    13921 ttcagtttca taatcaggcc agatgacttt taagaaatca tacacctctt ctgatgcaac
    13981 aagttgtttc gttttcataa tttccgatac tttcctctac ctttaaattc tattatttct
    14041 ttgtctctta atatttgaag ctgttgccta atcttttctt tgatatgatt gttcttaaca
    14101 aagatatttt ttaggtcact ttcaaaacga tacatatctt caagggtaaa ttctgaaccc
    14161 tctatcttat ctatacaatt tagaatttct attgtccaac ctcttgatga cagagagctc
    14221 ttccttaaaa ataaacctta cttaaattct tttgtaactt tttctggatc tctaacttgt
    14281 ccatcttgca caagaaatat ccttccttta gaaggtactt gtgataaatc aatgttacaa
    14341 ccaatccaac ctgctcgtct agcagttggt gcaagtggtt ttctttgaat aatcgatttc
    14401 ggtgtaacaa attgcttcgg aaggacaaga aagttattta cctcaaaatt ttttatgtaa
    14461 gttaaaaaaa agaaattagg attattatct gcctgcacac gcttcatcat cgttgcataa
    14521 gcaccatcat tgattgttga tgaaaaattt ccttttttgc tctttagttc aaactcctca
    14581 ctacaatgat tacagtaaaa atctgctaca ggccgattat tttcaaaatg atttaaggga
    14641 ttgttcccac aattagggca ataagactga cgataaaccc aatcctccgt taagatacgt
    14701 gtcttttggc tattactttt ataggtttct actaattcta aattaaagtg taattccatt
    14761 taaaaattcc tcactaatat gagaactcac tcgatataag caattaaaat ctactataac
    14821 tagtat
//
LOCUS       DAVLVP010000043        14138 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.043, whole genome shotgun sequence.
ACCESSION   DAVLVP010000043 DAVLVP010000000
VERSION     DAVLVP010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14138
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.043"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            1..843
                     /locus_tag="VYN69_001243"
                     /pseudo
     CDS             1..843
                     /locus_tag="VYN69_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611577.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(907..1989)
                     /locus_tag="VYN69_001244"
     CDS             complement(907..1989)
                     /locus_tag="VYN69_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007521532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1533515.1"
                     /translation="MKTKLPTEWQELSDQLGFQEFTPIQTQLFEPLLAGENLLGVSQT
                     GTGKTLAYLLPSLLRLQKKKAQQLLILAPNTELAGQIFDVCKTWAEAIGLTAQLFLSG
                     SSQKRQIERLKKGPEILIGTPGRIFELIKLKKIKMMNVETIILDEFDQLLDDSQIHFV
                     EKITHYAPRDHQLVYMSATTKFDQEKIVPNTRTIDLSDQKLDNIQHFYMQVDQRHRVD
                     MLRKLAHVEDFRGLVFFNSLSDLGNAEEKLQYRDILAVSLASDVNVKFRKIILEKFKD
                     NQLTLLLATDLLARGIDIDSLECVVNFDIPRDSETYTHRAGRTGRMGKEGYVITLVTH
                     PEEIKKLKKFASIREIVLKNQELYIK"
     gene            complement(1986..2963)
                     /locus_tag="VYN69_001245"
     CDS             complement(1986..2963)
                     /locus_tag="VYN69_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEV1533516.1"
                     /translation="MLKLGVIGTGAISHHFIEAAHTSGEYQLVAIYSRKLETAATFAS
                     RYQNIQLFDQLEVFFKSSFDLVYIASPNSLHFAQAKAALSAGKHVILEKPAVTQPQEW
                     FDLIQTAEKNNCFIFEAARNYHEKAFTTIKNFLADKQVLGADFNYAKYSSKMPDLLTG
                     QTPNVFSDRFAGGALMDLGIYPLYAAVRLFGKANDATYHAQQLDNSIDLNGDGILFYP
                     DYQVHIKAGKNITSNLPCEIYTTDGTLTLNTIEHIRSAIFTDHQGNQVQLPIQQAPHT
                     MTEEVAAFAHMIQQPDLNLYQTWLYDAGSVHELLYTMRQTAGIRFEAEK"
     gene            3196..3573
                     /locus_tag="VYN69_001246"
                     /pseudo
     CDS             3196..3573
                     /locus_tag="VYN69_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
     gene            3808..5199
                     /locus_tag="VYN69_001247"
     CDS             3808..5199
                     /locus_tag="VYN69_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013193417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEV1533517.1"
                     /translation="MNKSRLGRGRHGKTRHVLLALIGILAISICLLGGFIAFKIYQQK
                     SFEQKIESLKKEKDDQLSEGNQKEHFRQGQAEVIAYYPLQGEKVISSVRELINQDVKD
                     KLESKDNLVFYYTEQEESGLKGVVNRNVTKQIYDLVAFKIEETEKTSLGKVHLTEDGQ
                     PFTLDQLFSDASKAKEQLIKELTSFIEDKKIEQDQSEQIVKNFSDQDLSAWNFDYKDS
                     QIILYPSPVVENLEEIALPVSAFFDVIQSSYLLEKDAALYQSYFDKKHQKVVALTFDD
                     GPNPATTPQVLETLAKYDIKATFFVLGKNVSGNEDLVKRIKSEGHVVGNHSWSHPILS
                     QLSLDEAKKQITDTEDVLTKVLGSSSKLMRPPYGAITDDIRNSLDLSFIMWDVDSLDW
                     KSKNEAAILTEIQHQVANGSIVLMHDIHSPTVNALPRVIEYLKNQGYTFVTIPEMLNT
                     RLKAHELYYSRDE"
     gene            5302..5379
                     /locus_tag="VYN69_001248"
                     /pseudo
     CDS             5302..5379
                     /locus_tag="VYN69_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            5455..6297
                     /locus_tag="VYN69_001249"
     CDS             5455..6297
                     /locus_tag="VYN69_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEV1533518.1"
                     /translation="MDTYQLNNGVEIPVLGFGTFKAKDGEEAYRAVLEALKAGYRHID
                     TAAIYQNEESVGQAIKDSGVPREELFVTTKLWNSQQTYEQARQAFEESLEKLGLDYLD
                     LYLIHWPNPKPLRENDQWKIRNAEVWRAMEDLYQEGKIRAIGVSNFLPHHLDALLETA
                     KILPAVNQIRLAPGVYQEEVVAYCREKGILLEAWGPFGQGELFDSKQVQEIAANHGKS
                     VAQIALAWSLAEGFLPLPKSVTVSRIQANLDCFGIELSHEEHETLKTIAVHSGAPRVD
                     DMDF"
     gene            6570..7331
                     /locus_tag="VYN69_001250"
     CDS             6570..7331
                     /locus_tag="VYN69_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1533519.1"
                     /translation="MENLYLVKDDSQLAAFRDFVVRNTEKLKDYQSFLKNELAVCDLP
                     QAVIWSNFNAATQIIRESAVPAYTNNRRMVMTPDLAVWKELYLYQLMDYECSEQTQAI
                     ESHYHSLSENFLLQIVGHELAHWSEHFLDDFDGYDSYIWFEEGMVEYISRKYFLTEEE
                     FQAEKICNQSLVELFQKKYGWHSLNDFGSSTYDKNYASIFYEYWLSFLIVDKLVENLG
                     SVQAVFDSYHLWANTEKTFPLLDWFVQQKLIEKEI"
     gene            7526..8443
                     /gene="glyQ"
                     /locus_tag="VYN69_001251"
     CDS             7526..8443
                     /gene="glyQ"
                     /locus_tag="VYN69_001251"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018163989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEV1533520.1"
                     /translation="MSKKLTFQEIILTLQQFWNDQGCMLMQAYDNEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPSNIQELYLESLEK
                     LGINPLEHDIRFVEDNWENPSTGSAGLGWEVWLDGMEITQFTYFQQVGGLATGPVTAE
                     VTYGLERLASYIQEVDSVYDIEWADGVKYGEIFIQPEYEHSKYSFEISNQEMLLENFD
                     KFEKEAGRALEEGLVHPAYDYVLKCSHTFNLLDARGAVSVTERAGYIARIRNLARVVA
                     KTFVAERKRLGYPLLDEETRVKLLAEDAE"
     gene            8655..10691
                     /locus_tag="VYN69_001252"
     CDS             8655..10691
                     /locus_tag="VYN69_001252"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEV1533521.1"
                     /translation="MTKNLLVELGLEELPAYVVTPSEKQLGEKMAAFLKENRLSFEAI
                     QTFSTPRRLAVRVTGLSDKQSDLTEDFKGPAKKIALDSDGNFTKAAQGFVRGKGLTVE
                     DIEFREIKGEEYVYVTKEEVGQAVEAIVPGVVDVLKSLTFPVSMHWAGNSFEYIRPVH
                     TLTVLLDEQEFDLDFLDIKGSRVSRGHRFLGKETKIHSALSYEEDLRKQFVIADPCER
                     EQMIVDQIKEIEAKHGVRIEIDADLLNEVLNLVEYPTAFMGSFDAKYLEVPEEVLVTS
                     MKEHQRYFVVRDQDGKLLPNFISVRNGNAERLKNVIKGNEKVLVARLEDGEFFWREDQ
                     KLVISDLVEKLNNVTFHEKIGSLREHMIRTGQITVLLAEKAGLSVDETVDLARAAAIY
                     KFDLLTGMVGEFDELQGIMGEKYTLLAGETPAVAAAIREHYMPTSAEGELPESKVGAV
                     LAIADKLDTILSFFSVGLIPSGSNDPYALRRATQGVVRILDAFGWHIAMDELIDSLYA
                     LKFDSLTYENKAEVMDFIKARVDKMMGSTPKDIKEAVLAGSNFVVADMLEAASALVEV
                     SKEEDFKPSVESLSRAFNLAEKAEGVATVDSALFENDQEKALAEAVETLVLSGPASQQ
                     LKQLFALSPVIDAFFENTMVMAEDQAVRQNRLAILSQLTKKAAKFACFNQINTK"
     gene            10733..10990
                     /locus_tag="VYN69_001253"
     CDS             10733..10990
                     /locus_tag="VYN69_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002983550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEV1533522.1"
                     /translation="MDPKKIARINELAKKKKTEGLTLEEKVEQAKLREEYIEGYRRAV
                     RHHIEGIKIVDEEGNDVTPEKLRQVQREKGLHGRSLDDPNS"
     gene            complement(11213..12457)
                     /locus_tag="VYN69_001254"
     CDS             complement(11213..12457)
                     /locus_tag="VYN69_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000673688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1533523.1"
                     /translation="MKFVGLVGSNYDQSYNRKLLEFIRRNFKFKFELEVLEIDEVPMF
                     NQDEKWDESFQLRYLYNKITRADGVIIATPEHNHTISASLKSVLEWLSYEVHPFENKP
                     VMIVGASYYDQGTSRAQVHLRKILDAPGVNAYTLPGNEFLLGKAKEAFDNNGNITNEG
                     TVKFLETCLDNFVKYVGVVSKLKKPKPIESEDLDCGKPIATTITEVDPDDPEWVEKVA
                     AITGAVSGDTYVKLDHGILTVNQIDMFLKAMPFELTYADDNNQFLYYNNAHQDPDTMF
                     AKRVPPQSGSRMSTVHGSLPPARMKNVEWVIGTLRNGNQEYVRTIVPGSPAGVINTHN
                     YQAMYYPDGSYAGINEIVFNFQPWLDWYLKETGQRLVGGSGPFAPAAGGHGDADATSG
                     ASDSGDAGGHGGDADATAGASY"
     gene            complement(12475..13080)
                     /locus_tag="VYN69_001255"
     CDS             complement(12475..13080)
                     /locus_tag="VYN69_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEV1533524.1"
                     /translation="MLKLIAIVGTNSKRSTNRQLLQYMQKHFADKAEIELVEIKAIPV
                     FNKPADKQVPAEILEIAAKIEEADGVIIGTPEYDHSIPAVLMSALAWLSYGIYPLLNK
                     PIMITGASYGTLGSSRAQLQLRQILNAPEIKANVLPDEFLLSHSLQSFNPSGDLVDLD
                     VIKKLDAIFDDFRIFVKITEKLRNAQELLRKDAEDFDWENL"
     gene            complement(13140..14063)
                     /locus_tag="VYN69_001256"
     CDS             complement(13140..14063)
                     /locus_tag="VYN69_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEV1533525.1"
                     /translation="MTLRSHSERLMGTTITISLVDEQADIFLQKSFDLLKELEYRFNA
                     NSQESELMEINYQAGIAPVTVHPDLFELISLGLEHSLALSSHLNISIGPLIQTWRIGF
                     SDAKVAQPQEIESVLPLINPHDIELDSSTSTVFLKQKGMKIDLGCLAKGYSADKVAQF
                     LRKEGVTSALINLGGNILTIGKNQARGDNPWQIGIQDPANPRGNHLMTIPVVNKSVVT
                     SGIYERHLTVDGQDYHHIFDSQTGYPVETELASLTIISDKSVDGEIWTTRLFGERPAS
                     ILWQVESLEGIEVILIDKEGHLSCSSGIPTL"
BASE COUNT         4283 a         2569 c         3119 g         4167 t
ORIGIN      
        1 tcggactcga ctctctcctt caagaaacac gtggtggtcg taaccatgca tatatgacag
       61 ttgaggaaga gaaagccttt cttgcccgcc atttgaaggc tacagaggca ggagaatttg
      121 ttacaatacc ttatttcagg cttatatcgt tcctacacac gtgatgcctt ctatcaactg
      181 ttgaagcacc atggttggcg aaatattatg ccacgtccag aacatcctaa gaaagcagac
      241 gcttaaacca ttgtcgcgtc taaaaataaa atctcaattc aagaagacaa gaaagcgctt
      301 taaaaccagt agaccttttc ataaggttcg cttgatgtac caagatgagg ctggtttcgg
      361 tagaatcagt aaactgggat cttgttggtc tccaatagga gtaggtccac atgtccatag
      421 tcactatata cgagaatttc gctattgtta tggagctgtt gatgcccata caggcgaatc
      481 atttttctta atagctggtg gatgtaatac tgagtggatg aacgcctttt tagaagagct
      541 ttcacaagct tatccagatg attatctttt actcgttatg gacaatgcta tatggcataa
      601 atcaagtacc ttaaagattc cgactaatat tggttttacc tttattcctc catacacacc
      661 agagatgaac ccattgaaca agtgtggaaa gagattcgta aacgtggatt taagaataaa
      721 gcctttcgaa ctttggaaga tgtcatgaat caactccaag atgtcataca aggattggag
      781 aaggaggtga taaagtccat cgttaatcgg agatggacta gaatgctttt tgaaaacaga
      841 tgagtataaa attgaattgc ttataaaaaa gctccataca ctggatgtgt atagagcaat
      901 ggggctttat ttgatataga gttcttggtt ttttaggaca atttctcgga tacttgcaaa
      961 ctttttaagt tttttgattt cttctggatg agtgacgaga gtgataacat aaccttcctt
     1021 gcccatacga ccagtacggc cagcacggtg tgtgtaggtt tcgctatctc taggaatatc
     1081 aaagtttacg acacattcta ggctatcgat atcaattcca cgagccaaaa ggtcagttgc
     1141 aagaagcagg gttagttggt tatctttaaa cttttctaag atgatttttc taaatttaac
     1201 attaacatca ctagcgaggg aaacagccaa tatatcacga tactgtagtt tttcctcggc
     1261 attcccaagg tctgacaggc tattgaagaa gactagacca cggaaatcct ctacatgagc
     1321 cagttttcgt agcatatcca ctcgatgacg ttggtctacc tgcatgtaga aatgctggat
     1381 attgtccaat ttttgatcag agagatcaat agtgcgtgta ttcggcacaa tcttttcttg
     1441 gtcaaacttg gtcgtggcac tcatgtagac cagttggtgg tcacgaggtg cgtagtgagt
     1501 gattttttct acaaagtgaa tctgagaatc atctagtaat tggtcaaatt catccaggat
     1561 gatggtttcc acattcatca tcttgatttt tttaagttta atgagttcaa agatacggcc
     1621 aggagttcca atcagaattt ctggcccctt tttaagacgt tcaatttgtc gtttctgact
     1681 tgaacctgaa aggaagagtt gagcagtcaa tccgatagct tctgcccacg ttttacatac
     1741 atcaaaaatc tgtccagcaa gttccgtatt tggtgctaga atcaagagtt gttgggcttt
     1801 tttcttttgt agtctgagaa gacttggtag gagatacgct agggtcttac cagttccggt
     1861 ttggctcact cctaggaggt tttctccagc aagaaggggc tcaaatagtt gagtttgaat
     1921 gggggtgaat tcttggaaac cgagttggtc actcagctct tgccattcag tcggtagttt
     1981 ggttttcatt tttctgcctc aaatctaatg ccagcagtct ggcgcatggt atatagtagc
     2041 tcatgaacag agcctgcatc atacagccaa gtttggtaga gattcagatc tggttgctgg
     2101 atcatgtgtg caaatgcagc gacttcctca gtcatcgtat gaggagcctg ttggatagga
     2161 agctggactt gatttccttg gtggtcggta aaaatagctg agcgaatatg ctcaatcgtg
     2221 ttgagagtca aggttccatc tgttgtataa atctcgcaag gaagattgga agtgatgttt
     2281 tttccagcct tgatgtgaac ttgatagtct gggtagaaga ggataccatc tccatttagg
     2341 tcaatgctat tgtcaagctg ttgagcatgg taagtcgcgt cattggcttt tccaaaaaga
     2401 cgaacagcag catagagggg ataaatcccc aaatccatga gggctccacc agcaaaacgg
     2461 tctgaaaaga catttggtgt ttgtccagtc aacaagtcag gcatcttgga agagtatttg
     2521 gcatagttga aatctgctcc taacacttgc ttatctgcta aaaagttttt gatagtagta
     2581 aaggctttct cgtggtaatt acgagctgct tcaaagataa aacagttatt tttttcagct
     2641 gtttgaatca aatcaaacca ttcttgtggt tgagtgacag ctggcttttc gagaataaca
     2701 tgtttaccag cagacaaggc agcttttgcc tgagcaaaat gtaaggagtt tggactggcg
     2761 atatagacta aatcaaaaga agatttgaag aagacttcta attgatcgaa tagttggata
     2821 ttctgatagc gagaagcaaa ggttgctgca gtttctagtt ttctagaata gattgcgacc
     2881 agttggtatt ctccactggt atgggctgct tctatgaaat gatggctgat agcgccagtt
     2941 ccgatgacac ctaattttag cataaatact ccttttccga ttttaaatcc ttctttcatt
     3001 ataacataga tagacgggac tatccaatag agaggagaaa atttcaaata agctattagc
     3061 tttcttctga agaaaagctt tcttttccga ataaatagat agaagcatag aatctagcaa
     3121 acctagattt aaaaatgtgc tataatagaa ggaggaaaag gaggattctc agacatccgg
     3181 gtatcagccc aactaatgat ttgtcaattt atccgcgata tgctggactt gccagcaaaa
     3241 aatgtgacga ttttggaggg aagtaacatt cacgtcttgc cttccatgcc ctactcagcg
     3301 caggatttct atactagtat agacgtcttg gcggagttag ataatggaat ccaagttatc
     3361 atcgaaattc aggttcatca tcagaatttt ttcatcaatc gcctatgggc ttatctgtgt
     3421 agttaggtta atcaaaacct agaaaaaatt cgccaacgtg aaggtgatac ccaccagagc
     3481 tacaaacaaa tcgcactagt atacgctatc gcaattgtcg atagtaatta cttctcagat
     3541 gacctagctt ttcatagttt tatagtaaaa tgaaatgaga acaggacaaa tcgatcagga
     3601 cagtcaaatc gatttctaac aatgttttag aagtataggt ctactattct agcttcaatc
     3661 tactagaaat tccatagata gaaaactaca taatctctac agatacggat gttggagttg
     3721 atgtaagatg ctttggcttg ctagaggaat tgtggattgc caaattgtat cattgaaatt
     3781 attgctcaaa tttgttatga tataaatatg aataaaagta gactaggacg tggcagacac
     3841 gggaaaacga gacatgtatt attggctttg attggtattt tagcaatttc tatttgccta
     3901 ttaggcggat ttattgcttt taagatctac cagcaaaaaa gttttgagca aaagattgaa
     3961 tcgctcaaaa aagagaaaga tgatcaattg agtgagggaa atcagaagga gcattttcgt
     4021 caggggcaag ccgaagtgat tgcctattat cctctccaag gggagaaagt gatttcctct
     4081 gttagggagc tgataaatca agatgttaag gacaagctag aaagtaagga caatcttgtt
     4141 ttctactata cagagcaaga agagtcaggt ttaaagggag tcgttaatcg taatgtgacc
     4201 aaacaaatct atgatttagt tgcttttaag attgaagaga ctgaaaagac cagtctagga
     4261 aaggttcact taacagaaga tgggcaacct tttacacttg accaactgtt ttcagatgct
     4321 agtaaggcta aggaacagct gataaaagag ttgacctcct tcatagagga taaaaaaata
     4381 gagcaagacc agagtgagca gattgtaaaa aacttctctg accaagactt gtctgcatgg
     4441 aattttgatt acaaggatag tcagattatc ctttatccaa gtcctgtggt tgaaaattta
     4501 gaagagatag ccttgccagt atctgctttc tttgatgtta tccaatcttc gtacttactc
     4561 gaaaaagatg cggccttgta ccaatcttac tttgataaga aacatcaaaa agttgtcgct
     4621 ctaacctttg atgatggtcc aaatccagca acgaccccgc aggtattaga gaccctagct
     4681 aaatatgata ttaaagcgac tttctttgtg cttgggaaaa atgtttctgg gaatgaggac
     4741 ttggtgaaga ggataaaatc tgaaggtcat gttgttggaa accatagttg gagccatccg
     4801 attctctcgc aactatctct tgatgaagct aaaaagcaga ttactgatac tgaggatgtg
     4861 ctaactaaag tgctgggttc tagttccaaa ctcatgcgtc cgccttatgg agctattaca
     4921 gatgatattc gcaatagcct cgatttgagc tttattatgt gggatgtgga tagtctggac
     4981 tggaagagta aaaatgaagc agctattttg acggaaattc agcatcaagt agctaacggc
     5041 tctatcgttt tgatgcatga tattcacagt ccgacagtca atgccttgcc aagggtcatt
     5101 gagtatttga aaaatcaagg ttataccttt gtgaccatac cagagatgct caatactcgc
     5161 ctaaaagctc atgagctgta ttatagtcgt gatgaataag caagaaaaaa taggtctgtc
     5221 agatatgtga tagacttatt ttttacaaaa gatagtacta cttaaaaaat gttttatgct
     5281 ataattgatg aataatactc aatgaaaatc aaagagcaaa ctaggaaact agctgcaggt
     5341 tgctcaaaac agtgttttga ggttgtggat agaactgacg aagtcagtaa caatacctac
     5401 ggcaaggcga cgttgacgtg gtttgaagag agtataaaat agaaggagaa gcatatggat
     5461 acctatcaat taaataatgg agtagaaatt ccagtattgg gatttggaac ttttaaggct
     5521 aaggatggag aagaagccta tcgtgcagtg ttagaagcct tgaaggctgg ttatcgtcat
     5581 attgatacgg cggcgattta tcagaatgaa gaaagtgttg gtcaagcaat caaagatagc
     5641 ggagttccac gagaggaatt gttcgtaact accaagctgt ggaatagcca acaaacctat
     5701 gagcaagctc gtcaggcttt tgaagagtct ttagagaaac tgggcttgga ttatttggat
     5761 ctgtatttga ttcattggcc aaatccaaaa ccgctcagag aaaatgacca atggaaaatc
     5821 cgaaatgcag aagtttggag agcgatggaa gacctctatc aagaagggaa aatccgtgct
     5881 atcggtgtta gtaattttct tccccatcat ttggatgcct tgcttgaaac agcaaaaatt
     5941 cttccggcgg tcaatcaaat ccgcttggcg ccaggtgtct atcaagagga agttgtagct
     6001 tactgtagag aaaaaggaat tttattggaa gcttggggac cttttggtca gggagaactg
     6061 tttgatagca agcaagtcca agaaatcgca gcaaatcatg gaaagtctgt tgctcagata
     6121 gccttggcct ggagcttggc agaaggattt ttaccacttc cgaaatctgt tacagtatct
     6181 cgtattcagg ctaatcttga ttgctttgga attgaattga gccatgagga acatgaaacc
     6241 ttaaaaacga tagctgttca ttctggtgct ccacgagttg atgatatgga tttttagaaa
     6301 atcataaaaa gaattgtaca ttattctaat ttttgatata atagtcagca ggaaagaaag
     6361 tcttatggcg ttctttaagc gagcttggga tagtgggagc caagtagagc aaaataaaga
     6421 actggcgctt tctgtactat tttcaaaaac aatgaagtaa taaattaggg tggaaccgcg
     6481 tttctgacgc ccctaggtta aatcaaccta ggattgtcag atgtggttct tttgcttatt
     6541 cagtctattg tgtgaaagaa aggagagacg tggaaaacct ttatcttgta aaagacgata
     6601 gtcaactagc tgcatttcgt gattttgtag taagaaatac tgaaaagttg aaagattatc
     6661 aatctttttt aaagaatgaa cttgcagtct gtgatttacc gcaagccgtt atttggtcaa
     6721 attttaatgc tgctacacag attattaggg aaagcgctgt tccagcctat acaaataata
     6781 gacgaatggt tatgacgcct gatttagctg tttggaaaga gttgtatttg tatcaattga
     6841 tggactacga gtgttctgag caaactcaag caatagaaag tcactatcat tctttatctg
     6901 aaaatttcct cttacagatt gtaggacatg agttagctca ttggtcggag cattttttag
     6961 atgattttga tggttatgac tcttatatct ggttcgaaga ggggatggtt gaatatatta
     7021 gtcgcaagta tttcttgaca gaagaggaat ttcaagcgga aaaaatttgt aatcaatctc
     7081 tagtagaact ttttcagaag aagtatggtt ggcattcatt gaatgatttt ggttcttcga
     7141 cttatgataa gaactatgca agtatttttt atgaatattg gctcagtttt ttgatagtag
     7201 ataagttggt agaaaattta ggtagtgtac aagcggtctt tgattcttat catctatggg
     7261 caaatacaga aaaaactttt cccttgttag attggtttgt tcagcagaaa ttaattgaaa
     7321 aagaaatata aaaactaaag gagtaaacaa tgtctaagaa attaacattt cactgcatca
     7381 gtggcagaga cctccttaca gtcgggctgc tccacgctca gcactagagt gcctgagcta
     7441 gacgcagtac taactcgtct tgcctcgtat aatcgacgag gcagactcgt gtcgcaagaa
     7501 attatttttt attaaggagt attcaatgtc taagaaatta acatttcaag aaattatttt
     7561 gactttgcaa caattttgga atgaccaagg ttgtatgctt atgcaggctt atgataatga
     7621 aaaaggtgcg ggaacaatga gtccttacac tttccttcgt gctatcggac ctgagccatg
     7681 gaatgcagct tatgtagagc catcacgtcg tcctgctgac ggtcgttatg gggaaaaccc
     7741 taaccgtctc taccaacacc accaattcca ggtggtcatg aagccttctc catcaaatat
     7801 ccaagaactt taccttgagt ctttggaaaa attgggaatc aatcctttgg agcacgatat
     7861 tcgttttgtt gaggacaact gggaaaaccc atcaactggt tcagctggtc ttggttggga
     7921 agtttggctt gacggaatgg aaatcactca gttcacttat ttccaacaag tcggtggatt
     7981 ggcaactggc cctgtgactg cggaagttac ctatggtttg gagcgcttgg cttcttacat
     8041 tcaagaagta gactctgtct atgatatcga gtgggctgat ggtgtaaaat acggagaaat
     8101 ctttatccag cctgagtatg agcactcaaa atattcattt gaaatttcga accaagaaat
     8161 gttgcttgaa aactttgata agtttgaaaa agaagctggt cgtgcattag aagaaggctt
     8221 ggtacaccct gcctatgact atgttctcaa atgttcacat acctttaatc tgcttgacgc
     8281 gcgtggtgcc gtatctgtaa cagagcgtgc aggctatatc gctcgtatcc gtaacttggc
     8341 ccgtgttgta gccaaaacct ttgttgcaga acgcaaacgc ctaggttacc cacttttgga
     8401 tgaagaaaca cgagttaaac ttttagcaga agacgcagaa taaagagagt gacaaattac
     8461 gaaaatgggc gaacagagtg agccctgagc cagttgccac agtgatgaag gtatccttag
     8521 tgaaactaag gatactaggc aaaattggag acttttggct ccaattttag caatgaaaca
     8581 acgaagttgg ttgcttgcgt gccaatcaca taaggcaaac tggaaaataa aaagatactt
     8641 ttcggagaaa aaacatgaca aaaaacttat tagtagaact cggtcttgaa gaattaccag
     8701 cctatgttgt cacaccaagt gaaaaacaac taggcgaaaa aatggcagcc ttcctcaagg
     8761 aaaaccgcct gtcttttgaa gccattcaaa ccttctcaac accacgtcgt ttggctgttc
     8821 gtgtgactgg tttatcagac aaacagtctg atttgacaga agatttcaag ggaccagcaa
     8881 agaaaattgc cttggatagt gatggaaact tcaccaaagc agctcaagga tttgtccgtg
     8941 gaaaaggctt gactgttgaa gatatcgaat tccgtgaaat caagggtgaa gaatatgtct
     9001 atgtcactaa ggaagaagtt ggtcaagcag ttgaagccat tgttcctggt gttgtagatg
     9061 tcttgaagtc attgactttc cctgtcagca tgcactgggc tggaaatagc tttgaataca
     9121 ttcgtcctgt ccacacttta actgttctct tggatgagca agagtttgac ttggatttcc
     9181 ttgatatcaa gggaagtcgt gtgagccgtg gccatcgttt cttggggaaa gaaaccaaaa
     9241 ttcattcagc attgagctat gaagaagacc ttcgtaagca gtttgtaatc gcagatccat
     9301 gtgaacgtga gcaaatgatt gttgaccaaa tcaaggaaat tgaggcaaaa catggtgtac
     9361 gtatcgaaat tgatgcggat ttgctgaatg aagtcttgaa tttggttgaa tacccaactg
     9421 ccttcatggg aagttttgat gctaaatacc ttgaagttcc agaagaagtc ttggtgactt
     9481 ctatgaagga acaccagcgt tactttgttg ttcgtgatca agatggaaaa ctcttgccaa
     9541 acttcatttc tgttcgtaac ggaaacgcag agcgtttgaa aaatgtcatc aaaggaaatg
     9601 aaaaagtctt ggtagcccgc ttggaagacg gagaattctt ctggcgtgaa gaccaaaaat
     9661 tggtgatttc agatcttgtt gaaaaattaa acaatgtcac cttccatgag aagattggtt
     9721 cccttcgtga acacatgatt cgtacgggtc aaatcactgt acttttggca gaaaaagctg
     9781 gtttgtcagt ggatgaaaca gttgaccttg ctcgtgcagc agccatttac aagtttgact
     9841 tgttgacagg tatggttggt gaatttgacg aactccaagg aattatgggt gaaaaataca
     9901 cccttcttgc tggtgaaact ccagcggtgg cagctgctat tcgtgaacac tacatgccta
     9961 catcagctga aggagaactt ccagagagca aggtcggcgc agttctagcc attgcagaca
    10021 aattggatac gattttgagt ttcttctcag taggattgat tccatcaggt tctaatgacc
    10081 cttatgccct tcgtcgtgca actcaaggtg tggttcgtat cttggatgcc tttggttggc
    10141 acattgctat ggatgagctg attgatagcc tttatgcatt gaaatttgac agtttgactt
    10201 atgaaaataa agcagaggtt atggacttta tcaaggctcg tgttgataag atgatgggct
    10261 ctactccaaa agatatcaag gaagcagttc ttgcaggttc aaactttgtt gtggcagata
    10321 tgttggaagc agcaagtgct ctcgtagaag taagcaagga agaagatttt aaaccatctg
    10381 ttgaatcact ttctcgtgcc tttaacctgg ctgagaaggc agaaggggtt gctacggttg
    10441 attcagcact atttgagaat gaccaagaaa aagctttggc agaagcagta gaaacactcg
    10501 ttttatcagg acctgcaagt cagcaattga aacaactctt tgcgcttagc ccagtcattg
    10561 atgctttctt tgaaaatact atggtaatgg ctgaagatca ggctgtccgt caaaatcgtt
    10621 tggcaatctt gtcacaacta accaagaaag cagctaagtt tgcttgtttt aaccaaatta
    10681 acactaaata aaatttgata aacggacttt atcttattac agaggagaag aaatggatcc
    10741 gaaaaaaatt gctcgtatca atgagcttgc taaaaagaaa aaaacagaag gcttaacact
    10801 agaagaaaaa gtggaacaag ccaaactacg tgaggagtac atcgaaggtt atcgtcgcgc
    10861 tgttcgtcat cacattgaag gaatcaaaat tgtggacgaa gaaggaaacg atgttacacc
    10921 agaaaaacta cgccaagtac aacgtgaaaa aggattacat ggccgtagtc ttgatgaccc
    10981 aaattcataa taatactctt cgaaaatcaa attcaaacca cgtcagcttc accttgccgt
    11041 acttaagtac agcctgcggc tagcttccta gtttgctctt tgattttcat tgagtataag
    11101 tattctttct tttaacaagg atagatgaaa cgataacaaa gagactagca gtttgtgttt
    11161 gctagtcttt tttcgctaaa aaaggaacca taatggttcc taaaaactat cattagtaac
    11221 ttgcaccggc tgtagcgtct gcgtcaccac cgtggcctcc agcatcccct gaatcagaag
    11281 cgccagaagt agcatcggcg tctccatgac ctccggcagc aggagcaaat ggtccgctac
    11341 cacccaccaa acgttgacca gtctctttta ggtaccagtc aagccatggt tggaagttaa
    11401 agacgatttc attgatacca gcgtatgatc catcaggata gtacattgct tggtagttgt
    11461 gagtgttgat aacacctgca ggagaacctg gaacgatcgt acggacgtat tcttggtttc
    11521 cgttgcgaag tgttccgata acccactcta cgttcttcat acgtgctggt ggaagagaac
    11581 catgaacagt cgacatacgg ctacctgatt gaggtggtac acgtttagcg aacatagtgt
    11641 ctggatcttg gtgagcgttg ttgtagtaga ggaattggtt gttgtcgtca gcgtatgtca
    11701 attcaaatgg catagctttc aagaacatat caatttggtt aactgttagg ataccgtggt
    11761 ccaatttgac ataggtatca ccagaaacag caccagtgat tgctgcaact ttttctaccc
    11821 attctggatc gtcagggtca acttctgtga tggttgtagc gattggtttt ccacaatcca
    11881 agtcttctga ttcgattggt tttggttttt tcaatttcga aacgactcct acgtatttaa
    11941 caaagttatc taagcaagtt tcaaggaatt taacagtgcc ttcgttggtg atatttccgt
    12001 tgttatcaaa agcttcctta gctttaccaa gaaggaattc gttacctgga agcgtgtagg
    12061 cattaacacc tggagcatca aggattttac gaaggtgaac ttgagcacgt gatgttcctt
    12121 ggtcatagta tgatgcaccc acaatcataa caggcttgtt ttcaaatgga tgaacttcgt
    12181 atgaaagcca ttcaagtaca gatttgagtg aagctgagat agtgtggtta tgctcaggag
    12241 tagcaatgat aacaccatct gcacgagtaa ttttgttata taaataacgc aattggaagc
    12301 tttcgtccca tttttcgtct tggttaaaca ttggaacttc gtcgatttca agtacttcta
    12361 attcaaattt gaatttgaaa ttgcgacgga taaattccaa gagtttgcgg ttatatgatt
    12421 gatcgtagtt tgatccaaca agtccaacaa atttcattct ttcggtctcc tatcttacaa
    12481 attttcccag tcaaagtctt ctgcatcttt gcgaagcaat tcttgcgcat tgcgcaattt
    12541 ctcagtaatc ttcacgaaga tacggaagtc atcaaagatg gcatccaatt tcttgataac
    12601 atcaaggtca accaagtcgc cacttgggtt aaatgattga agagagtgtg agagcaagaa
    12661 ttcatctgga agaacatttg ccttgatttc aggagcattc aagatttgac gaagttgcaa
    12721 ttgggcacga gatgaaccaa gcgtaccgta agaagcacct gtaatcatga ttggtttgtt
    12781 caaaagtggg tagataccgt aagatagcca agcaagagca ctcatcaaaa cagctggaat
    12841 agagtgatca tactcaggag taccgataat aacgccatct gcctcttcga ttttagcagc
    12901 aatttccaat atttcagcag gtacttgctt gtcagctggt ttgttgaaga caggaatggc
    12961 cttgatttca acaagctcaa tttcagcttt gtcagcaaag tgtttttgca tgtattgaag
    13021 caattgacgg tttgtagaac gttttgaatt tgttccaaca atagcaataa gttttaacat
    13081 gagatttcct ttctcttttt acataataca attttaaaat tccattgaaa cagttgtctc
    13141 tatagagtag gaattcctga agaacagctt aggtggcctt ctttatcgat gaggataact
    13201 tcgatgccct ccaaactttc gacttgccag aggatagaag caggtctttc tccaaagagt
    13261 cgagtcgtcc agatttcgcc atcgactgat ttatcagaga tgattgttag actcgctagt
    13321 tccgtttcaa caggatatcc tgtttgactg tcaaaaatgt gatggtaatc ttgtccatcg
    13381 acggtcaggt gacgttcata aatgcctgaa gtcacgacag atttattgac aacagggatg
    13441 gtcattaaat gatttcccct aggattggct gggtcttgaa tcccgatttg ccatgggtta
    13501 tcccctcttg cctgattttt tccaatggtc aggatattcc ctcccagatt gatcaaggca
    13561 gaagtcaccc cctctttcct aagaaattgg gcaaccttat ccgcactgta tcctttggct
    13621 aaacaaccta gatcgatctt cattcctttc tgttttaaaa acacagtaga agtagaagaa
    13681 tctaactcga tatcatgagg attgattaga ggcagcaccg attcaatctc ttgaggctgg
    13741 gcgaccttgg catctgaaaa accgatacgc caggtttgaa ttaagggacc aatgctgata
    13801 ttgaggtggc tagagagcgc taggctatgc tctaacccaa gtgaaatcag ctcaaacagg
    13861 tctggatgaa ccgtgacggg ggctattcct gcttgataat tgatttccat caactcagat
    13921 tcttgactat tggcgttgaa gcggtattca agttctttga gcaagtcaaa ggacttttgg
    13981 agaaagatat cggcttgctc atccactaat gaaatagtga tagtagtccc cattagccgt
    14041 tcagaatgtg aacgaagagt caagctacca actcctttct cttatagaaa ataagttgta
    14101 atatcaaata attatctaaa ttgaagccct tacatttt
//
LOCUS       DAVLVP010000044        14121 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.044, whole genome shotgun sequence.
ACCESSION   DAVLVP010000044 DAVLVP010000000
VERSION     DAVLVP010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 14121)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14121)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14121
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.044"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(231..1838)
                     /locus_tag="VYN69_001257"
     CDS             complement(231..1838)
                     /locus_tag="VYN69_001257"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEV1533526.1"
                     /translation="MSTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPY
                     INIDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRK
                     ERRGEYLGATVQVIPHITDALKEKIKRAALTTDSDVIITEVGGTVGDIESLPFLEALR
                     QMKADVGGDNVMYIHTTLLPYLKAAGEMKTKPTQHSVKELRGLGIQPNMLVIRTEEPA
                     GQGIKNKLAQFCDVAPEAVIESLDVEHLYQIPLNLQAQGMDQIVCDHLKLDAPAADMT
                     EWSAMVDKVMNLKKQVKISLVGKYVELQDAYISVVEALKHSGYVNDAEVKINWVNAND
                     VTAENVAELLSDADGIIVPGGFGQRGTEGKIQAIRYARENDVPMLGVCLGMQLTCIEF
                     ARHVLGLEGANSAELAPETKYPIIDIMRDQIDIEDMGGTLRLGLYPSKLKRGSKAAAA
                     YHNQEVVQRRHRHRYEFNNAFREQFEAAGFVFSGVSPDNRLVEIVEIPENKFFVACQY
                     HPELSSRPNRPEELYTAFVTAAVENSN"
     gene            complement(2228..2815)
                     /locus_tag="VYN69_001258"
     CDS             complement(2228..2815)
                     /locus_tag="VYN69_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000418402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEV1533527.1"
                     /translation="MELEVFAGQEKSELSMIEVARAILELRGRDHEMHFSDLVNEIQN
                     YLGTSNSDIREALPLFYTELNFDGSFISLGDNKWGLRSWYGVDEIDEEIIALEENDDD
                     EVAPKAKKKRVNAFMDGDSDAIDYNADDPEDEDAYEADPALSYDDENPDDEKNEVEAY
                     DAEINEIAPDDLGEDVDLNEDDDEFSDDDAETSEE"
     gene            complement(2943..3392)
                     /locus_tag="VYN69_001259"
     CDS             complement(2943..3392)
                     /locus_tag="VYN69_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000333655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1533528.1"
                     /translation="MCFVPYYKVNHCEEAFAWYQDVNLVYLVDGVKLPYSQATLEALY
                     SYLDRHGELFWIEVKEKGEWFPIGDITLSQDNLPIVIGNSAYQHRGLGKKILSALIEL
                     ARVKGWKELRVKEIYTYNHASRRCFKSLGFVENGATEKGRSFILELV"
     gene            complement(3397..3786)
                     /locus_tag="VYN69_001260"
     CDS             complement(3397..3786)
                     /locus_tag="VYN69_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParB"
                     /protein_id="HEV1533529.1"
                     /translation="MKVNIAVLHPTQLYLSEKKLQDIQMLYQSAETIQVGPISILAFG
                     DCLLITDGHHRAYQALLAGRDTISAEWDRDGGDELYHLYAQACEERKIYSVFDLEDRI
                     LAQDGYEAKWYNWCDGFNQAATLLLKR"
     gene            complement(3814..4440)
                     /locus_tag="VYN69_001261"
     CDS             complement(3814..4440)
                     /locus_tag="VYN69_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEV1533530.1"
                     /translation="MKGSFALLLFVILGYMVKFYPETLVGFDQSIQTAIRGDLPDYLT
                     ILFRSLTRLIDIPVIITWVVIIAFVFYRKRWKIESFFMLGNLALAGLLIVTFKNIYQR
                     PRPAILHLVEEKGFSFPSGHSLAVTLMVGTLIVILSQRIKDQVWRKIVQIVLGLYLVS
                     VLVSRVYLGVHYPSDVLASFCVGLGVLFIEFPFYDKLRFQWRFKGKQK"
     gene            complement(4454..5017)
                     /locus_tag="VYN69_001262"
     CDS             complement(4454..5017)
                     /locus_tag="VYN69_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1533531.1"
                     /translation="MTNTRRLSTIAILSAISFVLMYFDFPLLPAASFLKIEFSILPVL
                     VGLVVMDLPAALGVLLLRSLLKLLLNSQGVNTYIGLPMNIVALGVFVIVFALIWKKER
                     TTLRFLLGSLAGTVGLTLAMLVLNYVYAVPLYAKFANFDIGKILGLSNYLMTMVLPFN
                     LIEGVIFSVSFWLLYVLLKPTLKHYER"
     regulatory      complement(5182..5416)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(5470..5973)
                     /locus_tag="VYN69_001263"
     CDS             complement(5470..5973)
                     /locus_tag="VYN69_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HEV1533532.1"
                     /translation="MTNHIVLFEPQIPQNTGNIARTCAATNSPLHIIKPMGFPIDDRK
                     MKRAGLDYWDKLEIYFYESLEDFISQMKGKLYLISKFAEKVYSEVDLSTDEDHYFLFG
                     REDKGLPEDFMREHPEKALRIPMNDEHVRSLNVSNTVCMIVYEALRQQNFAGLELVHT
                     YEVDKLK"
     gene            complement(6166..6996)
                     /locus_tag="VYN69_001264"
     CDS             complement(6166..6996)
                     /locus_tag="VYN69_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1533533.1"
                     /translation="MQAKLIGYQHRDTVIHRLSGAGKLLFFILVSLAAMISYDTRMLL
                     LIAIFSVFLLYLSEIRFKDVSFVAVFATIFAVLNVLMVYLFSPEYGVGLYGERSVIWQ
                     GIGVYTLTSQELFYLLNLAIKYLCTIPLAIIFLMTTHPSQFASSLNQIGVPYKIAYSV
                     SLTLRYIPDLQEEFFTIKMSQEARGMELSKKASLMQRIKGNLLIITPLIFSSLERIDT
                     IATAMELRRFGKEKKRTWYSYQALKKGDYITLLLATLFLVVSLLLILQNQGRFYNPWK
                     "
     gene            complement(6981..8663)
                     /locus_tag="VYN69_001265"
     CDS             complement(6981..8663)
                     /locus_tag="VYN69_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533534.1"
                     /translation="MKEAIIEWKDFSFRYETQQEPTLQGIDLTIYKGEKVLIVGPSGS
                     GKSTLGQCLNGTIPNIYKGQTYGEFLIKGQVAFDMSIYDKSHLVSTVLQDTDGQFIGL
                     SVAEDLAFALENDVTALDEMKGRVYKWAEKLDLISLLNQRPQDLSGGQKQRVSLAGVL
                     IDESPILLFDEPLANLDPKSGQDIIELIDQIHKEEGTTTLIIEHRLEDVLHCPVDRIV
                     LINDGRILFNGSPDQLLATDLLTQNGIREPLYLTTLRQLGVDLVKEEQLADLDNLYIS
                     KGQVQLRTELVKETPELQSLFRLEDVSFSYDDRPILKSLHLDIKKGEKIAIVGKNGAG
                     KSTLAKALSSFIQTEGRYLWEGQDIKGDSVAERAERVGYVLQNPNQMISTNMIFDEVA
                     LGLRLRGVDEQEIETRVYETLKICGLYEFRNWPISALSFGQKKRVTIASILVLGAEII
                     LLDEPTAGQDQKNYTEIMEFLEELHQQGHTIVMITHDMQLMLDYSDRALVMVDGELIA
                     DTVPASLLSDPELLVKANLKETSIFNLAKKLDVDPLDLTAFYKERREGCKLN"
     gene            complement(8765..9067)
                     /locus_tag="VYN69_001266"
     CDS             complement(8765..9067)
                     /locus_tag="VYN69_001266"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533535.1"
                     /translation="MKIKEQTRKLAAGCSKQCFEIVDRTDEVSSKHGFEVVDETDEVS
                     SKHGFEVVDETDEVSNHTYGKAKLTWFEEIFEEYKMMGKAGQLVFFDVYRLVRQVS"
     gene            complement(9074..9622)
                     /locus_tag="VYN69_001267"
     CDS             complement(9074..9622)
                     /locus_tag="VYN69_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000403170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HEV1533536.1"
                     /translation="MEIKFTIKQVVAVGIGAALFVVIGMINIPTPVPNTSIQLQYAVQ
                     ALLSIIFGPIIGLLVGLIGHAIKDSLAGYGLWWTWIIASGLFGLVVGLFRKYVRVING
                     VFDWKDILIFNLIQLLANALVWGVLAPLGDVVIYQEAAEKVFAQGIVAGIANGVSVAI
                     AGTLLLLAYAGTQTRAGSLKKD"
     gene            complement(9637..10485)
                     /locus_tag="VYN69_001268"
     CDS             complement(9637..10485)
                     /locus_tag="VYN69_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosyl-l-methionine hydroxide
                     adenosyltransferase family protein"
                     /protein_id="HEV1533537.1"
                     /translation="MNNNLLVLQSDFGLVDGAVSAMIGVALEESPTLKIHHLTHDITP
                     YNIFEGSYRLFQTVDYWPEGTTFVSVVDPGVGSKRKSVVAKTAKNQYIVTPDNGTLSF
                     IKKHVGIVAIREISEVANRRQNTEHSYTFHGRDVYAYTGAKLASGHITFEEVGPELSV
                     DQIVELPVVATIIEDHLVKGAIDILDVRFGSLWTSITREEFYKLEPEFGDRFEVTIYH
                     ADMLVYQNQVVYGKSFADVRIGQPILYINSLYRLGLAINQGSFAKAYNVGVGSSWTIE
                     IKKIEG"
     gene            10673..12022
                     /gene="trkA"
                     /locus_tag="VYN69_001269"
     CDS             10673..12022
                     /gene="trkA"
                     /locus_tag="VYN69_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HEV1533538.1"
                     /translation="MKIILVGGGKVGFALCRSLVAEKHDVLLIEQDEAVLNHIVSRFD
                     IIGILGNGADFAILEQASVQDCDIFIALTEHDEVNMIAAVLAKKMGAKETIVRVRNPE
                     YSNSYFKEKNILGFSLIVNPELLAARAIANIIDFPNALSVERFAGGRVSLMEFVVKST
                     SGLCQMPISDFRKKFGNVIVCAIERDHQIIIPSGDMTVQDKDRIFVTGNRVDMILFHN
                     YFKSRAVKSLLIVGAGRIAYYLLGILKDSRIDTKVIEINPEIASFFSEKFPNLYIVQG
                     DGTAKDILLEESAQHYDAVATLTGVDEENLITSMFLDRVGVQKNITKVNRTSLLEIIN
                     APDFSSIITPKSIAVDTIMHFIRGRVNAQYSDLQAMHHLANGQIETLQFHIKEANKMT
                     AKPLSQLKLKKGVLIAAIIRKGKTIFPTGEDMLEVGDKLLVTTLLPNITKIYDLIAR"
     gene            12026..13465
                     /locus_tag="VYN69_001270"
     CDS             12026..13465
                     /locus_tag="VYN69_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1533539.1"
                     /translation="MNKSMIRYLLSKLLLIEAVLLLVPVAIAVYYRESSQVFTALFST
                     IGILVLLGGSGSLQKPKNQRIYAKEGVLIVALCWILWSFFGGLPFVFSGQIPSLIDAF
                     FEISSGFTTTGASILNDISVLSRSLLFWRSFTHLIGGMGVLVFALAIMDNAKNSHLEV
                     MKAEVPGPVFGKVVSKLKNTAQILYLLYIALFSLFVIIYYLAGMPLYDSFVIAMGTAG
                     TGGFTVYNDGIAHYGSSLITYLVSIGVLVFGVNFNLYYYLMLRRVKAFFGDEELRAYL
                     VIVLVSTGLISLNTLYLYPGFSKSFEMTFFQVSNIITTTGFGYGDITNWPLFSQFILL
                     FLMAIGGSAGSTAGGLKIIRGLILSKIAKNQILSILSPHRVLTLHVNKMVIDKDTQHK
                     ILKYFVIYAMILLALIFIVSLDSNDFLVVTSAVFSCFNNIGPILGTTSSFSIFSPISK
                     ILLSFAMIAGRLEIYPILLLFMKRTWSKR"
     gene            complement(13590..13847)
                     /locus_tag="VYN69_001271"
     CDS             complement(13590..13847)
                     /locus_tag="VYN69_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEV1533540.1"
                     /translation="MNEGAQLAEVRVIANSGAYGAHYDIMGVYDLIILAPQVRSYYRE
                     MKVDAERLGIQIVATRGMEYIHLTKNPSKALQFVLEHYQAV"
BASE COUNT         4137 a         3234 c         2620 g         4130 t
ORIGIN      
        1 atccagttcc taaaactttg ctagaaattg atttggcttt ccgaattaat ttgttcaaat
       61 tttatttcat ttcaatatac tgataaatcc attcactagc acagggcaag agggagtcgc
      121 tattgatttc aacggtagca ggtacataca gaggacccct acctcagata tactgcaata
      181 tcatcgtatg caaaaaacct ctgagatttt tctcaaaggt tctgattttg ctaattgctg
      241 ttctcaactg ctgcagtaac aaaggcagtg tagagttctt ctggtcggtt tggacggctt
      301 gacagttcag ggtgatactg acaagctaca aagaatttat tttcaggaat ttccacgatt
      361 tctaccaaac gattgtctgg agaaactcct gaaaagacaa aacctgctgc ctcaaactgc
      421 tcacggaagg cattattaaa ctcataacgg tgacggtgac ggcgttgcac cacttcttga
      481 ttgtgataag cagcagcagc cttagagcca cgtttcaact tagacggata aagtcccaaa
      541 cgaagggttc cacccatatc ctcaatatca atctgatcac gcatgatatc aatgataggg
      601 tattttgttt ctggtgcaag ctctgcagaa ttggcacctt caagacctaa aacgtgacga
      661 gcaaactcga tacatgtcaa ctgcattccc aagcagactc ccaacattgg aacatcattt
      721 tcacgcgcat agcggatggc ttggattttc ccttctgtac cacgttgacc aaaaccacct
      781 ggtacgatga tcccgtccgc atcagacaag agttctgcta cattctctgc tgtcacatca
      841 ttggcattga cccaattgat tttaacttct gcatcattga catagccaga gtgtttcaag
      901 gcttcgacca ctgagatata ggcatcttgc aactccacat acttaccaac aagggaaatc
      961 ttaacttgtt tcttgaggtt catgaccttg tccaccatgg ctgaccattc tgtcatatcc
     1021 gctgctggtg cgtctaattt caaatgatca caaacaattt ggtccatccc ttgtgcctgc
     1081 aagttcagtg gaatttggta aaggtgttca acatccaacg attcgataac ggcttctggt
     1141 gccacatcac agaactgggc cagtttattt ttaattcctt gaccagctgg ctcttctgta
     1201 cgaataacca acatatttgg ttggattccc aagccacgca attctttgac agagtgttgg
     1261 gttggtttgg ttttcatttc accagcagcc ttgaggtaag gaagcaaggt tgtatggata
     1321 tacatgacat tatccccacc cacatctgcc ttcatctgac gaagagcctc taggaatggc
     1381 aaggactcga tatctcctac tgttccacca acctctgtga taatgacatc agagtcggtc
     1441 gttagagcgg cacgcttgat tttttctttc aaagcatctg tgatatgagg aatgacttga
     1501 acagttgccc caaggtattc tccacggcgt tctttacgaa gaacttcact gtaaattttc
     1561 ccagttgtca cgttggaata tttgttgaga ttgatatcga tgaaacgttc atagtgaccc
     1621 aagtccaaat ctgtctcagc tccgtcatct gtcacaaaaa cttccccgtg ctggtaagga
     1681 ctcatggttc ccggatcaat attgatataa gggtcaaact tttgaatggt tactttgaga
     1741 ccacgatttt tcaagagacg gcctagactc gctgccacaa tccctttccc aatagacgat
     1801 accacaccac cagttacaaa aatatatttc gtagacatag attcctcttt ctaaaatgct
     1861 caaggtcttg ttaactacga ggggatatag aaaacaagac cctactaata agaataatct
     1921 gggacgactg caccagattc acaactaata ctcttcgaaa atctcttcaa accgcgtcaa
     1981 cgtcgccttg ccgtagatat gtgttcctga ctttgtcagt cttatctaca acctcaaaac
     2041 agtgttttga gcagcctacg gctagtttcc tagcttactc tttgattttc attgagtata
     2101 aaatacaaga agataacttc ttgaaaaaac aaaaatagct ccctaagaac tagggagccc
     2161 cgacctctaa aagaggtgcc caaacaatat gatacctaaa aataggataa ttgtcaatag
     2221 ctaactctta ttcctcgctg gtttcagcgt cgtcatctga aaactcgtcg tcgtcttcgt
     2281 tgagatccac atcttctccc aagtcatctg gagcgatttc gttgatttct gcatcataag
     2341 cttccacttc atttttttca tcatctggat tttcatcatc gtatgaaaga gctggatctg
     2401 cttcgtatgc atcttcgtct tctggatcat ctgcattgta gtcaatggca tctgaatcac
     2461 catccataaa ggcattgaca cgttttttct tagcttttgg tgctacttca tcgtcgtcat
     2521 tttcttcaag agcgatgatt tcttcgtcga tttcgtccac accataccat gaacgaagac
     2581 cccatttgtt gtccccaagt gagatgaagc taccgtcaaa gttcaactct gtgtagaaca
     2641 aaggcaaagc ttcgcggata tcgctgtttg atgttccaag gtagttttga atttcgttta
     2701 caagatcgct aaaatgcatc tcatgatcgc gaccacgaag ttctaagata gcacgcgcta
     2761 cctcaatcat agatagttca cttttttctt gcccagcaaa tacttctaat tccaaagcgt
     2821 ttctcctcat ttatactact atcgtcagag cgaacagact ctgacctcat tttatcattt
     2881 actctttatt ttacgataat tttgcggaat agtcaaaggt taagggggag aaagtggcag
     2941 gattagacta attccaatat aaaactcctt cctttttctg ttgctccatt ttccacaaat
     3001 ccaagcgact tgaaacacct cctagaagca tgattgtagg tgtagatttc cttgactctc
     3061 aattctttcc atccttttac tcgagccaat tcaatcaaag cacttagaat cttttttcca
     3121 agtcctcgat gttggtaagc ggaattccca atcacaatgg ggagattatc ctgagatagt
     3181 gtaatatccc caattggaaa ccattctccc ttctccttga cttcaatcca aaaaagctca
     3241 ccatgccgat ccaaatagga atacagggct tccaaggtcg cttgactgta aggaagcttc
     3301 accccatcta cgaggtaaac caagttcaca tcctgatacc aagcaaaagc ttcctcacaa
     3361 tgattgacct tataataagg aacaaaacac aatgcactac cttttcaaca aaagagttgc
     3421 tgcttgatta aaaccatcac accagttata ccattttgct tcatacccat cttgagctag
     3481 gatacgatct tctaaatcaa aaacagagta aatctttctt tcctcgcaag cttgcgcata
     3541 gagatgatat agttcatcac caccatctct atcccactca gcagaaatcg tatcccgacc
     3601 tgccaataaa gcctgataag ccctgtgatg cccatctgta atcagcaaac aatctccaaa
     3661 ggcaagaata ctgattggac cgacttggat tgtttctgcc gactggtaaa gcatctgaat
     3721 atcttgcaac ttcttttctg ataaatatag ttgagtcgga tgaagaactg ctatattgac
     3781 tttcatttct ttctcctcaa gggaattcga tactcacttc tgtttgcctt taaatcgcca
     3841 ttggaagcgg agcttgtcat aaaagggaaa ctcgataaac aagactccca aacccacaca
     3901 gaaactggca agaacatctg atgggtagtg aactcccaga tagactcttg ataccagcac
     3961 actgactagg tagaggccaa ggacgatttg tacgattttt ctccagacct gatctttaat
     4021 ccgctgacta agaataacaa tcaaagtccc taccatcagc gttacagcta gagaatgccc
     4081 acttgggaag gaaaatccct tctcctccac cagatgtaag atagctggtc gtgggcgctg
     4141 gtagatattt ttaaaggtca cgattaaaag acctgccaaa gccagatttc ccagcatgaa
     4201 gaaactttct atcttccatc gcttacgata aaagacaaaa gctataatga caacccaagt
     4261 gataatcact ggaatatcaa tcagacgtgt gagggatcga aaaagaatag tcaaataatc
     4321 tggtaagtct cctcgaatgg cagtctgaat cgattggtca aaaccgacca gcgtttcagg
     4381 gtaaaatttg accatgtagc caagaataac gaaaagtaaa agggcaaaac tgcccttcat
     4441 taaaaatgtt tgtttatctt tcatagtgtt ttaaggttgg tttcaagagg acgtacaaca
     4501 accagaatga aacggaaaag attacaccct caatcaagtt aaaaggtaat accatggtca
     4561 ttaggtagtt ggaaagtccc aaaatttttc caatatcaaa gttagcaaac ttagcgtaca
     4621 aaggaacagc ataaacatag ttgagaacca acatggccaa ggttaaacca acagttccag
     4681 ctagagagcc tagtaggaaa cgaagggttg tccgttcctt tttccaaatc aaagcaaata
     4741 cgatgacaaa aactcccaaa gctacgatat tcatcggcaa accaatgtaa gtattcactc
     4801 cttggctgtt aagaagcaat ttcaagagtg agcgaagcaa gagcactcct agagcagcag
     4861 gcaaatccat gaccaccaga cccacaagga ctggcaagat actaaattcg atcttgagga
     4921 aagatgccgc tggtaaaagc ggaaagtcaa agtacatcag cacaaatgag atggctgata
     4981 aaattgcaat ggtcgaaagt cgacgtgtgt ttgtcataac aggttcctcc aattttctat
     5041 aaaatcagaa gaagttggaa aggattcctc tatctattct cactttttat atcccaaaag
     5101 ttccctctta ctctattaaa gaaaaacaaa gcaagtggtt acaatccggc tataaatcta
     5161 tcaaaacaga caaagctatt ctttcgtctt ctcccatcca gactatactg tcggttgtgg
     5221 aatctcacca catcagcttg cgctcgcgga cttctttaaa gagaaggaaa tagagatttt
     5281 tcttttagac taggcgacaa gctgtaggca taacttctgg ttaggcaaag cttgtcaaca
     5341 aagtataaaa ggaaaagatc atttcctagt taaagtcacc gccggtcggg aatcacaccc
     5401 tgccctgaag actcttttat cataacaaaa aacgcttgca agcgcaagca ttttgttcat
     5461 tttcattttt tatttcaatt tatccacttc ataggtatgg acgagctcaa gacctgcaaa
     5521 gttctgctgg cgaagagcct catagacaat catgcagacg gtattagaca cattgagact
     5581 gcggacatgt tcatcattca tgggaatacg gagagccttc tcaggatgtt ctcgcataaa
     5641 atcttcaggt aagcccttgt cttcacgtcc aaagagaaaa taatggtcct cgtcagtcga
     5701 taaatccacc tcagaataga ctttttccgc gaatttcgaa atcagataga gttttccctt
     5761 catctgagat atgaaatctt ccaaactctc gtaaaaataa atctctagct tatcccagta
     5821 atccaatcca gcccgcttca tcttgcggtc atcaatagga aagcccatag gcttgatgat
     5881 gtggagggga gaattggtcg cagcgcaagt acgcgcgata ttgcccgtat tttgtggaat
     5941 ttgaggttca aataatacaa tgtgatttgt catgacttgc ttcctttcac cattgcaaaa
     6001 aaatagccac actgcccgga gtcaagctca gcaaacagcg tggttaaggc atcgttaact
     6061 tacctcacaa caggtttgaa gtaaatcagc gaaactactt tcttagtata acactttcag
     6121 aatcattgtc aatagaaatg acttgatttt ttcaattttt tcaagctatt tccaagggtt
     6181 gtaaaatcgt ccctgattct gcaagataag tagtaaacta actactaaaa acaaggttgc
     6241 caagagcaag gtaatatagt ctcctttttt caaggcctga taactatacc atgtgcgttt
     6301 tttctctttc ccaaagcggc gaagctccat ggcagtcgca atggtatcaa tgcgttctag
     6361 cgagctaaaa atcaagggcg taataatgag cagattgcct ttgattcgtt gcataagaga
     6421 agctttcttg gataattcca tcccacgcgc ctcctgagac atcttgatag taaagaattc
     6481 ttcctgcaaa tctggaatat agcgcaaggt caggctgaca gaataagcaa tcttataggg
     6541 cacaccaatt tgatttaaac tggaagcaaa ctgactagga tgggttgtca tcaaaaagat
     6601 aatagccaga ggaatggtgc aaagatactt aatggccaaa tttagcagat aaaagagctc
     6661 ctggctggtt agagtgtaga caccgattcc ctgccaaatc acacttctct ctccataaag
     6721 tccaacccca tactcgggag aaaagagata gaccatcaaa acgtttaaaa cggcaaatat
     6781 cgtcgcaaaa acggctacaa aggaaacatc tttaaagcga atttctgata aatagaggag
     6841 aaagactgaa aagatggcaa tcagcaagag cattctggta tcatagctaa tcatggccgc
     6901 caatgatacc agaatgaaaa agagaagttt cccagctcct gacaagcgat gaatcacagt
     6961 atctctatgc tggtaaccga ttaatttagc ttgcatccct ctctcctttc tttgtaaaat
     7021 gccgttaaat ccagtggatc cacatctagt ttcttagcca agttaaagat ggaggtttct
     7081 tttagattgg cttttactaa cagctcagga tcgctcaaca gactggctgg aacagtatcg
     7141 gcaatcaatt ctccatccac catgacaagg gctcgatctg aataatccag catcaattgc
     7201 atatcatggg taatcatgac aatggtatgc ccttgttgat gcagttcttc gagaaattcc
     7261 ataatctcag tatagttctt ctggtcttga cccgcagtcg gttcatctag gaggataatt
     7321 tcagcgccta agaccaaaat tgaggcaata gtcacacgtt ttttctgacc aaatgacagg
     7381 gcagaaatgg gccaattacg gaattcatag agaccacaga ttttcaaggt ttcatagact
     7441 ctcgtttcaa tttcctgctc gtccacacct cgcaaacgga gtcccagagc cacctcatca
     7501 aaaatcatat tggttgaaat catttgattg ggattttgca gcacatagcc tactcgttcc
     7561 gcccgctctg caacagaatc tccttttata tcctgtcctt cccaaagata gcggccttcc
     7621 gtctggataa agctactcaa ggccttggct agagttgact tccctgctcc attttttccg
     7681 acaatggcaa tcttttcacc ctttttaata tctaaatgaa gagattttaa aatcggtcta
     7741 tcatcataag aaaacgacac gtcctctagt ctaaagagtg actgcaattc tggggtttct
     7801 tttaccagtt ccgtccgcaa ctgaacctga ccttttgaga tatacaagtt atccagatct
     7861 gctaattgtt cttccttgac taaatccaca cccaattgac ggagagtggt gagataaagg
     7921 ggttcgcgaa ttccattttg ggtcaacaaa tcagtcgcca gtaactggtc agggctccca
     7981 ttaaaaagga tacgaccatc gtttatcaag acaatccgat ccacagggca atgcagaaca
     8041 tcctccaaac ggtgctcgat aataagggtc gtcgtcccct cctccttatg aatctggtca
     8101 atcaattcga taatatcctg acctgacttg ggatcaagat tggcgagtgg ctcatcaaac
     8161 aagagaatcg gactctcatc aatcaagaca ccagccagac tgactcgctg cttttgccca
     8221 ccagacaaat cctgaggacg ctgattcagt aaagaaataa ggtccagctt ttcagcccat
     8281 ttataaacac gacctttcat ctcatctagg gctgtcacat cattttccag agcaaacgcc
     8341 aaatcttctg ccacagacaa gccaataaac tgcccatctg tatcctgcaa aactgtgcta
     8401 accagatgag acttatcata gatgctcata tcaaaggcta cttgaccctt tatcaaaaat
     8461 tctccatatg tctgaccctt gtaaatattg ggaatagtcc cattcaaaca ctgacccaag
     8521 gtagatttac ctgatccaga tggtccaaca attaagactt tctctccctt gtaaatggtc
     8581 aagtctatcc cttgcaaggt cggttcttgt tgtgtttcat accggaaaga gaaatccttc
     8641 cactcaatta tagcttcttt catcttactc tcttcattcg cttcttagac ttctatttta
     8701 tcataaatca agcccttctt gcagtctctt ctcttaaaat cttagcgcca aaaagattcc
     8761 tatcctagct tacttgccta actaatctat aaacatcgaa aaagactagt tgcccagcct
     8821 tccccatcat tttatactct tcgaaaatct cttcaaacca cgtcagcttc gccttgccgt
     8881 aggtatggtt actgacttcg tcagtttcat ctacaacctc aaaaccatgt tttgagctga
     8941 cttcgtcagt ttcatctaca acctcaaaac catgttttga gctgacttcg tcagttctat
     9001 ccacaatctc aaaacactgt tttgagcaac ctgcggctaa cttcctagtt tgctctttga
     9061 ttttcattga gtattagtcc tttttcaaac ttcctgcacg agtttgagtt cctgcatagg
     9121 caagtaagag aagagttcct gcaatagcta cagatacacc attggcaatt cccgcaacaa
     9181 tcccttgtgc aaatactttt tctgccgctt cttgataaat cacaacatct ccaagtggtg
     9241 ccaagacacc ccaaacaagg gcatttgcaa gtagttgaat gaggttaaaa ataagaatat
     9301 ctttccagtc aaaaacacca ttgatcactc gaacgtactt tctaaaaagt cccacaacta
     9361 aaccaaagag tccgctagcg ataatccaag tccaccatag accataacca gcaagagagt
     9421 ccttgattgc atgaccaatc aacccgacaa gcaaaccgat aatcggtcca aaaataatag
     9481 aaagtagcgc ttgtaccgca tactgaagct ggatgcttgt atttggaaca ggggttggaa
     9541 tgttgatcat cccgatgacg acaaagaggg cagcgccaat tccgacagca acaacttgtt
     9601 taattgtaaa tttgatttcc atactattct cctattttat ccttctattt tctttatttc
     9661 aatggtccaa gatgaaccga cacctacatt ataggccttg gcaaaggaac cttggttgat
     9721 agccaaacct aaacgataga gagagttgat gtaaaggatg ggttgcccaa ttctcacatc
     9781 tgcaaatgat ttgccataga caacctgatt ttgatagacc agcatatcag catgatagat
     9841 ggtcacttca aaacgatcac caaattctgg ttccagcttg taaaattctt cccgtgtgat
     9901 agaggtccaa agcgaaccga aacgcacatc cagaatatca atggctccct tcaccagatg
     9961 atcttctatg atggtcgcta cgactggaag ctctacaatc tggtccacac tgagctctgg
    10021 ccctacttcc tcaaaagtaa tgtgaccact ggccagttta gcaccagtat aggcatagac
    10081 atcacgaccg tggaaggtat aagaatgctc tgtgttttga cgcctattgg ccacctcaga
    10141 aatctcacga atggctacaa tgccaacgtg tttcttgata aaggaaagcg tcccattatc
    10201 tggcgtgaca atgtattgat tttttgcagt cttggcaact acactcttac gtttcgaacc
    10261 gacacctgga tcgacaaccg atacaaacgt cgttccctca ggccagtaat ccaccgtctg
    10321 aaagagacga tagctcccct caaaaatatt ataaggcgtg atatcgtgcg tcaagtgatg
    10381 gatttttaag gttggagact cttctaaagc cactccaatc atagccgata ccgcaccatc
    10441 aaccagacca aagtctgatt gtaataccag taaattattg ttcattttat ctccttgtat
    10501 atcctttatc ataccatatt tcagagaaag tcgctaatag ctatttcaat tggttagggt
    10561 atagttttac cttatagcga attttcacta aaagctcttg ttattagcta gtaggtgcat
    10621 tttttcttga aaaagggtat gatagttaca tcatgaaaaa gtaggtttta ttatgaaaat
    10681 tatccttgtc ggagggggaa aagttggttt tgccctctgt cgctccttgg ttgcagaaaa
    10741 gcatgatgtt ttgctgattg agcaagacga agctgttctc aatcatattg tcagtcgctt
    10801 tgatatcatt ggtatccttg gtaacggggc cgattttgcc attcttgagc aagccagcgt
    10861 ccaagattgt gatatcttta tcgccctgac tgagcacgat gaagtcaaca tgattgcagc
    10921 agttctagcc aagaaaatgg gagctaaaga aactatcgtt cgggtgagga accctgaata
    10981 ttctaactct tatttcaagg aaaagaatat tctcggtttt tctcttatcg ttaatcctga
    11041 gctcttggct gcccgcgcta tcgcgaatat cattgacttc cccaacgccc tgtctgtcga
    11101 acgctttgct ggtggacgcg ttagcctcat ggaatttgtc gtcaagtcca ccagcggtct
    11161 ttgccaaatg cccatttctg attttcgtaa aaaatttggt aatgtcattg tctgtgcgat
    11221 agagagggat catcaaatta tcattccaag cggtgacatg actgtacagg ataaagatag
    11281 aatctttgtc actggtaacc gtgtcgatat gatactcttc cataattatt ttaaatcacg
    11341 cgccgtgaag agccttctca tcgttggagc aggtagaatt gcctattatc tacttggtat
    11401 tctcaaagat agtcgtatcg atacaaaagt cattgaaatc aatcctgaaa tcgccagctt
    11461 ctttagcgag aaattcccaa atctctacat cgttcaagga gatggaaccg caaaagatat
    11521 cctgctggaa gaaagtgctc aacactatga tgccgttgcg actctaacag gagtcgatga
    11581 ggaaaatctg attacatcta tgttccttga cagggtaggt gtacagaaaa atattactaa
    11641 ggtcaatcgt accagtctcc tcgagattat caatgcgcct gatttttcaa gtatcatcac
    11701 acctaaaagc atcgctgtag atacgattat gcactttatt cgtggtcgag ttaatgccca
    11761 gtattcagac cttcaagcca tgcaccatct agccaatggc caaatcgaaa ccctgcaatt
    11821 ccatatcaag gaagccaata aaatgactgc caaacctctt tctcaactga aattgaaaaa
    11881 aggggttctt attgcagcca ttattcgaaa gggcaagact atttttccaa ctggggagga
    11941 tatgttggaa gttggagaca agctcctagt aacaaccttg ttgccaaaca tcaccaagat
    12001 ttatgacttg attgcgaggt aagaaatgaa taaaagtatg attcgttacc tcctttcaaa
    12061 gttacttttg attgaagctg ttctcctctt ggttcctgtg gctatcgctg tctattaccg
    12121 tgaatcgagc caagtcttta cagccctctt ttcgaccata gggattctcg tattgttagg
    12181 cggttcaggg agtttacaga agccaaaaaa tcaacggatt tatgccaagg agggagtctt
    12241 gatcgttgcc ctctgttgga tcctttggtc tttctttggt ggtctcccct ttgtcttttc
    12301 tgggcaaatc cctagcctta ttgatgcctt ttttgaaatt agttctgggt ttacaactac
    12361 tggagcaagt attttgaacg acatttcggt tctcagccgt tccctcctct tctggcgaag
    12421 ttttacccac ttgattggag ggatgggagt gcttgttttt gcacttgcta ttatggacaa
    12481 tgccaaaaat agtcacctag aagtaatgaa ggctgaggtt cctggtcctg tctttggtaa
    12541 ggttgtatcc aaattaaaaa acactgccca gatcctctat ctcctttaca tagccctctt
    12601 ttccctcttt gtcatcatct attatctagc cggtatgcct ctctacgata gttttgtcat
    12661 tgctatgggg acagcgggaa ctggaggatt taccgtctat aacgatggaa ttgcccacta
    12721 tggtagctca ctgattacct atctggttag tatcggagtt ttggtttttg gggtaaattt
    12781 caatctctac tactacctta tgctccgtcg ggttaaggcc ttctttggag atgaagagct
    12841 tcgggcttac ttggtcatcg tgctggtttc tacaggcttg attagcctca atactctcta
    12901 cctctaccca gggttttcaa agagctttga aatgaccttc ttccaggttt ccaacatcat
    12961 tacaacaact ggttttggat atggagatat taccaactgg cccctcttct cccagtttat
    13021 cctccttttc ctcatggcaa tcggtggctc tgctggatca actgcaggtg gactcaagat
    13081 tattcgaggc ctcatccttt caaaaattgc caaaaaccaa attttgtcaa tcctatcgcc
    13141 ccaccgtgtt ttgaccctcc atgttaataa aatggtgatt gacaaagata cccagcataa
    13201 gattctcaag tactttgtca tctatgctat gattttgcta gcgcttatct ttattgtcag
    13261 cctagatagc aatgattttc tggtcgtgac cagcgctgtc tttagctgtt tcaataatat
    13321 cgggcctatt ctaggaacca cttctagttt ctcaatcttt agtcccatct caaaaattct
    13381 cctctccttt gcaatgattg cagggcgctt ggagatttac ccaatcctcc ttctctttat
    13441 gaaaagaact tggtctaaga gataaaatac aaaatccctc tttttacaat tgtgaaagag
    13501 ggatttttat attaaaaagg gggaacgttt ttaaatccaa gcctgtctgt aagcaaagtt
    13561 cttactcaaa taaaagatgg aaaaacttac tacacagctt ggtaatgctc caatacaaat
    13621 tgtaaggctt tacttggatt ctttgttaaa tgaatatatt ccattcctct ggtagcaact
    13681 atctgaatac ctaatctttc tgcatccacc ttcatctctc tataataact ccgaacttgt
    13741 ggagccagaa taattaaatc ataaacaccc ataatatcat aatgagctcc gtacgctcct
    13801 gaattcgcaa tcactctaac ttctgctaat tgagcgccct cgttaattgc attggctaat
    13861 tacgcacttg ttccagaccc tgcacaaaga accagtactt tcaactcctt actttttatt
    13921 acatctatct caccaggcat tatttcactt gtatcctcct ctaaaataat atcatttgag
    13981 cttgcaatat ctacaacctg tagtagatat ctcgtattta gacaatatga aaacaagacg
    14041 acttccatat aggaaaccgc cttctctcta tgttgagtga tttatattaa aataactttt
    14101 cttctagctg cattttatta t
//
LOCUS       DAVLVP010000045        13872 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.045, whole genome shotgun sequence.
ACCESSION   DAVLVP010000045 DAVLVP010000000
VERSION     DAVLVP010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13872
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.045"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            487..1653
                     /locus_tag="VYN69_001272"
     CDS             487..1653
                     /locus_tag="VYN69_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEV1533541.1"
                     /translation="MKITEYTKKDGSIVYRSSVYLGIDTVTGKKVKTTISGRTKRELR
                     NKALQAQIDFEKNGSTVFKAVQINTYAELVENWLETYCHTVKKATLTIIKSRLKNYLL
                     PAFGDYKLDKLTPPVIQKQVNQWAKEYNQLGKGYQEYPQLNSLNKRILKYAVSLQVIP
                     FNPARDIIVPRRKAKEGQKLKYLDDDNLKKFLNYMEQLPNTYKNFYDTVLYKTLLATG
                     LRIRECLALKWSDIDLQNGTLDVNKTLNCEKEVTTPKTKSSVRMIDLDNKTVLMLRLY
                     KNRQAQIGREIGLTYKKVFSNSFDEYRDARALRSRLEKHLKLSECPRLTFHAFRHTHA
                     SILLNAGLPYKEIQTRLGHSQISITMDTYSHLSKDNKKNATSFYEKAIEKLKSS"
     gene            1796..2596
                     /locus_tag="VYN69_001273"
     CDS             1796..2596
                     /locus_tag="VYN69_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000938258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEV1533542.1"
                     /translation="MLPYPFSFFSSIWGFRKPLSKRFGLNWFQLLFTSIFLISLSMVP
                     IAIQNSSQETYPLETFIDNVYEPLTNEVVQDLSEHATIVDGTLTYTGTVSQASSVVIG
                     PSQIKELPKDLQLHFDTNELVISKESKELTRISYRAIQTESFKSKDSLTQAISKDWYQ
                     QNRVYISLFLVLGASFLFGLNFFIVSLGASLLLYITKKSRLFSFRTFKECYHFILNCL
                     GLPTLITLILGLFGQNMTTLITVQNILFVLYLVTIFYKTHFRDPNYHK"
     gene            2606..3592
                     /locus_tag="VYN69_001274"
     CDS             2606..3592
                     /locus_tag="VYN69_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001145427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1533543.1"
                     /translation="MPVTIKDVAKAAGVSPSTVTRVIQNKSTISDKTKKRVRKAMKEL
                     NYHPNLNARSLVSSYTQVIGLVLPDDSDAFYQNPFFPSVLRGISQVASENHYAIQIAT
                     GKDEKERLNAISQMVYGKRVDGLIFLYAQEEDPLVKLVAEEQFPFLILGKSLSPFIPL
                     VDNDNVQAGFDATEYFIKKGCKRIAFIGGSKKLFVTKDRLTGYEQALKHYKLTTDNNR
                     IYFADEFLEEKGYKFSKRLFKHDPQIDAIITTDSLLAEGVCNYIAKHQLDVPVLSFDS
                     VNPKLNLAAYVDINSLELGRVSLETILQIINDNKNNKQICYRQLIAHKIIEK"
     gene            3728..3808
                     /locus_tag="VYN69_001275"
                     /pseudo
     CDS             3728..3808
                     /locus_tag="VYN69_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(3884..4825)
                     /locus_tag="VYN69_001276"
     CDS             complement(3884..4825)
                     /locus_tag="VYN69_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1533544.1"
                     /translation="MKQTKRIKRWRYYLRRFAYQIKILRVLQSISREKYDEKISASLV
                     YGFLSAVAVNFFFQPGHVYSSGATGLAQIISALSNHWFGFHIPISLSFYAINFPLMVL
                     AWYQIGHKFTVFTFITVSMSSFFIQFVPVATLTEDPIINSLFGGVVMGLGIGFALRNN
                     ISSGGTDIVSLTIRKKTGKNVGSISFLVNGTIMLIAGLTFGWKYALYSMITIFVSSRV
                     TDAVFTKQKRMQAMIVTNHPEKVIEKIHKKLHRGATMIHDAEGTYNHERKAVLITVIT
                     RAEFNEFKQIMTQVDPSSFVSVSENVHILGRFVEIDN"
     gene            complement(4803..6566)
                     /gene="aspS"
                     /locus_tag="VYN69_001277"
     CDS             complement(4803..6566)
                     /gene="aspS"
                     /locus_tag="VYN69_001277"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEV1533545.1"
                     /translation="MKRSMYAGRVREEHIGQEITLKGWVGRRRDLGGLIFIDLRDREG
                     IMQLVINPEKVSAEVMATAESLRSEFVIEVTGQVAAREQANDKLPTGAVELNVTALIV
                     LNTAKTTPFEIKDGIEANDDTRLRYRYLDLRRPEMLENLKLRAKVTHSIRNYLDELEF
                     IDVETPFLSKSTPEGARDYLVPSRVNKGHFYALPQSPQITKQLLMNAGFDRYYQIVKC
                     FRDEDLRGDRQPEFTQVDLETSFLTEQEIQDITEGLIARVMKETKGIEVTLPFPRMKY
                     DDAMALYGSDKPDTRFDMLLQDLTEVVKGVDFKVFSEALAVKAIVVKGAADNYSRKDI
                     DKMTEVAKQYGAKGLAWVKVVDGELNGPVAKFLTGIQEELTTALALEDKDLVLFVADT
                     LEVANATLGALRGRIAKELGLIDNDKFNFLWVVDWPMFEWSEEEGRYMSAHHPFTLPQ
                     EETAHELEGDLAKVRAIAYDIVLNGYELGGGSLRINQKDLQERMFKALGFSAEEANDQ
                     FGFLLEAMDYGFPPHGGLAIGLDRFVMLLAGEENIREVIAFPKNNKATDPMTQAPSTV
                     ALKQLEELSLQVEEDETNKTN"
     gene            complement(6802..6882)
                     /locus_tag="VYN69_001278"
                     /pseudo
     CDS             complement(6802..6882)
                     /locus_tag="VYN69_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            complement(6918..7148)
                     /locus_tag="VYN69_001279"
     CDS             complement(6918..7148)
                     /locus_tag="VYN69_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000832182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533546.1"
                     /translation="MKRVILLAVIQAVVLFFIIGALAYAFKGDFFYNYLAVVFAPIAG
                     VLRFGTAYITEIVLPRKAAEIAEKRKAGKNSK"
     gene            complement(7166..7807)
                     /locus_tag="VYN69_001280"
     CDS             complement(7166..7807)
                     /locus_tag="VYN69_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1533547.1"
                     /translation="MKLLKNLGWILLALLSFFFIYGFIQGLATASLALGASPYAVTLL
                     YVVLAGVYVYGIYKWYQKAPVYIEKSGFNRFIWLPALVWFLSLVVQFFLPDDPSVNQQ
                     IATDLTLSQPLFSFFAVVIFAPLTEEIVFRGMLARYLFPKQDNSKRTLIFLLVSSLLF
                     ALIHFPGDVQQFFVYFSLGFSLGLAYVSRKGLVYSISLHALNNLVGFLMILML"
     gene            complement(7819..8553)
                     /locus_tag="VYN69_001281"
     CDS             complement(7819..8553)
                     /locus_tag="VYN69_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HEV1533548.1"
                     /translation="MKKKRRLLFLMSIVLGGFLGMFVGMFKARVESHEIILDVKALMP
                     WISAICLLIGFISMFLTFNFLKKSRKFHSLYQEEMDDDLNETYYVQMYRNLEFGTIAF
                     NITGVAIPLAIFISLSEVIILHTNPQTFFLSFLLFVVFLVAQKSLFKTIAIVRQFDLE
                     FFATPKDVLNYINSYDEGERQANLEQSFRILFQLHQYVLPALYIFLIIISFLTGEIQL
                     LAFLLVGAIHVYINVMQLPMVKRYFK"
     gene            complement(8556..8753)
                     /locus_tag="VYN69_001282"
     CDS             complement(8556..8753)
                     /locus_tag="VYN69_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001046145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533549.1"
                     /translation="MNKYKVIYYVVVIALLVSVFLLIGMDLSWFNPYQSDQFVWVYFA
                     LIPVTEWIEKKSKNLASEKGE"
     gene            complement(8753..8959)
                     /locus_tag="VYN69_001283"
     CDS             complement(8753..8959)
                     /locus_tag="VYN69_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1533550.1"
                     /translation="MQLKNRLKELRARDGLNQTDLAKLAGVSRQTISLLERDEYTPSI
                     IIALKISQIFNETVESVFRLEEDE"
     gene            complement(8969..9283)
                     /locus_tag="VYN69_001284"
     CDS             complement(8969..9283)
                     /locus_tag="VYN69_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533551.1"
                     /translation="MWVLGFILFMIFFYSNNSKKIKKLENKIKKLERKEKGNAEMSRL
                     LQEMIGKEPIITGVYIGPDNWEVVDVDEEWVKLRSVDNTGKEKFKLQRIEDIQTVEFD
                     GE"
     gene            complement(9554..9634)
                     /locus_tag="VYN69_001285"
                     /pseudo
     CDS             complement(9554..9634)
                     /locus_tag="VYN69_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(9625..10914)
                     /locus_tag="VYN69_001286"
     CDS             complement(9625..10914)
                     /locus_tag="VYN69_001286"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEV1533552.1"
                     /translation="MKLQKPKGTQDILPAESAKWQYVEGFAREIFKRYNYAEVRTPIF
                     EHYEVISRSVGDTTDIVTKEMYDFYDKGDRHITLRPEGTAPVVRSYVENKLFAPEVQK
                     PSKFYYMGPMFRYERPQAGRLRQFHQIGVECFGSSNPATDVETIVMAAHFLKEIGIQG
                     VKLHLNTLGNPESRAAYRQALIDYLTPLKETLSKDSQRRLEENPLRVLDSKEKEDKVA
                     VENAPSILDFLDEESQAHFDAVRQMLENLGVDYIIDTNMVRGLDYYNHTIFEFITEIE
                     GNDLTVCAGGRYDGLVAYFGGPETAGFGFGLGVERLLLILEKQGVALPIENALDVYIA
                     VLGDGANVKALELVQALRQQGFKAERDYLNRKLKAQFKSADVFATKTLITLGESEVES
                     GQVTVKNNQTREEVQVSLETISQNFSEIFEKLGFYTQ"
     gene            complement(10992..12239)
                     /locus_tag="VYN69_001287"
     CDS             complement(10992..12239)
                     /locus_tag="VYN69_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HEV1533553.1"
                     /translation="MKLLFRNPAYRILTLSRFFNAFGASIFNLVFIVYASTLSQASFA
                     VAMANIVMILPTFFTVFAGIRADYTRDKVKWIVYSGLFQAVLFFLAALVVQQASLFAF
                     SSLCLINVISDIISDFAGGLRMPLIKEKVAEDDLMEAYSFSQFITYISAIGGQAFGVW
                     LLDLSVNNFSLVAGINACFFLVSATILFLGKSKLSLSMSSADGENLKNEKLSIKDQFL
                     TIYRNLRLVFLKSGQKNFGFMLFAVLLINSLGGALGGIYNIFFLSHSLLNFSYTEALF
                     INQFCVLVAIIISSLTGNAYFGKQSLPRLMMWATVGLSLVGLANLFNQVVLGLLFLFF
                     TLYVSGKVQPKISAMLMKNLAPEVLAHTSNFLGLLFTLSIPVGTACFSLVAVWNIQLT
                     WMLFVGLSLLAIFLTILNLKNDI"
     gene            complement(12430..13281)
                     /gene="rgg"
                     /locus_tag="VYN69_001288"
     CDS             complement(12430..13281)
                     /gene="rgg"
                     /locus_tag="VYN69_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434947.1"
                     /note="Rgg, as the term is used in Streptococcus
                     pneumoniae, is the lone quorum-sensing system
                     transcriptional regulator related to proteins Rgg1, Rgg2,
                     Rgg3, and Rgg4 of Streptococcus pyogenes; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system transcriptional regulator
                     Rgg"
                     /protein_id="HEV1533554.1"
                     /translation="MEQIGKVFRQLRESRNISLRQATGGQFSPSMLSRFETGQSELSV
                     EKFLFALENISASVEEILFLARGFQYDTDSELRKEITDVLEPKNIAPLEDLYRREYQK
                     HAHSHNKQKHILNAIMIKSYMKSIDERVDLTAEEGKVLHDYLFSTEIWGIYELNLFSV
                     SSPFLSVSLFTRYVREMVRKFDFLMEMSGNRNLFHTILLNGFLASIECEEFTNAYYFK
                     RVIEEHFYKENETYFRIVYLWAEGLLDSKQGRVKEGQKKIEDAVRIFEMLGCNKSAEY
                     YRNTTEC"
     gene            13405..13746
                     /locus_tag="VYN69_001289"
     CDS             13405..13746
                     /locus_tag="VYN69_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003080443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1533555.1"
                     /translation="MRDDIKINDRALALQDQIIEKLEKVFDTDVELDVYNLGLIYEIN
                     LDETGLCKIVMTFTDTACDCAESLPIEIVAGLKQIEGIKDIKVEVTWSPAWKITRISR
                     YGRIALGLPPR"
BASE COUNT         4404 a         3014 c         2534 g         3920 t
ORIGIN      
        1 tatcactgct cataataata agcttttcaa ttataaatgt cgtgagaatc ttccacaact
       61 agcttatttc cactattaca ataacaatgc caatacatca agacaatctg tatattatgt
      121 ttttcttgcc ttgcaatgcc tacttgcttc cacacaatac gctcaattac ataacactat
      181 tattaaaaga accaaagttc taaataaaaa agcgcactcc attgaagccg gcattgtatt
      241 ggatacactt ggatttccga ataattggta taatatcact gacacattaa gatagaacta
      301 cttgcaatat atgggctttt aaaaaatccc atataagccc catatccgcc ttgtttccta
      361 ctctggtatt atttaccgtt tgactaatta aaatcaaaaa taaggggctt ctcgtaactc
      421 ctcgcatggt ataaactcaa aaccttttct aattgcttgc ctgctgatgg aaaaaggagt
      481 aaaaccatga aaataacaga atatactaaa aaagacggct caatagtcta tcgctctagc
      541 gtctaccttg gcatcgatac cgtaacgggt aagaaagtca agacgactat atcagggcga
      601 accaagagag aacttagaaa caaagcgcta caggctcaaa tagactttga aaaaaacggc
      661 tctacagtat ttaaagcggt tcaaatcaat acctatgccg aactggtgga gaattggctg
      721 gaaacatact gccatacggt caaaaaggca acccttacaa taatcaagag tagactaaaa
      781 aactatctac ttccagcctt tggagactac aaactagata agctaacacc cccagttatt
      841 caaaagcaag ttaatcaatg ggcaaaagag tacaatcaac taggaaaagg ctaccaagaa
      901 tatccgcaac ttaactcgct aaataaacgc atacttaaat acgccgtatc cttgcaagtt
      961 atccctttta atcctgcccg tgatattatc gtcccacgtc gtaaggcaaa agaagggcaa
     1021 aaactcaaat atctggatga tgataactta aaaaaattct taaattatat ggagcagctg
     1081 ccaaacactt acaaaaattt ctacgatacg gtgctatata agacgctttt agcgactggt
     1141 ttgcgcattc gtgaatgttt agccctcaaa tggtctgata ttgacttaca aaacggtaca
     1201 ctagacgtaa ataagactct aaactgtgaa aaagaagtca ctacccctaa aacaaaatca
     1261 agcgttagaa tgattgactt ggataataag acggtactca tgctgcggct ttacaaaaat
     1321 agacaagcgc aaattggtag agaaataggc ttgacttata aaaaagtatt ttctaattcc
     1381 tttgacgaat atagagacgc tagggcgctt aggtcaagac ttgagaaaca tttaaaactt
     1441 tctgagtgcc ctcgtttaac gttccacgct ttccgccaca ctcacgctag tatcttactc
     1501 aatgctggac tgccttacaa agaaatacaa acaaggctag ggcattctca aatatcaata
     1561 accatggata cttatagcca cttgtccaaa gacaacaaga aaaacgctac ttcattttat
     1621 gaaaaagcca ttgaaaaatt aaaaagttcc taaaaagtcc acaaatttta taaatcggct
     1681 ataaatcaag ccacaaatac cgctatacta gggaatttac cccctagact tgaaataaag
     1741 cgcatttctc tatataataa tactcatata gaaaacacct tttagaaaga tacctatgct
     1801 tccatatcca ttttcctttt tttcaagtat ttgggggttt cgtaagcccc tgtccaaacg
     1861 tttcgggctc aactggtttc aacttctctt taccagtatc ttccttatca gcttgtctat
     1921 ggtacccatt gctatccaaa acagctccca ggagacctat ccgctagaaa cttttatcga
     1981 taatgtctat gaacctctga caaatgaggt tgtccaggat ctctctgaac atgctacaat
     2041 tgtcgatggc acattaactt atactggaac agttagtcaa gcctcttctg ttgtgattgg
     2101 tccaagtcaa atcaaggaat tacctaagga cttgcaactg catttcgata caaatgagct
     2161 agtcatcagc aaggaaagca aggaactgac ccgcatctct taccgagcca ttcagactga
     2221 gagtttcaaa agcaaagaca gcttgaccca agcaatttct aaagactggt accaacaaaa
     2281 tcgtgtctat atcagcctct tcctagttct cggtgcgagc ttcctctttg gtttgaattt
     2341 ctttatcgtc tctctaggag ctagtcttct cctttatatc actaagaaat cacgcctctt
     2401 ttcatttaga acctttaaag agtgctatca ctttatcttg aactgtttag gattgccgac
     2461 tctgattaca cttattttgg gattatttgg ccaaaatatg acaaccctga ttactgtaca
     2521 aaatattctt tttgttctgt atctggtcac tatcttttat aaaacacatt tccgtgatcc
     2581 aaattaccat aaataggaga tttttatgcc cgttacgatt aaagacgtgg ccaaggctgc
     2641 tggtgtttcg ccttcaaccg taacccgtgt tattcaaaat aaatcaacca ttagcgacaa
     2701 aacaaaaaaa cgcgttcgca aagctatgaa ggaactcaac taccacccaa acctcaacgc
     2761 tcgtagcttg gtaagcagct atactcaggt tatcggatta gttcttcctg atgactcaga
     2821 cgccttctac cagaatcctt tctttccatc ggttctacgt ggcatctctc aagtcgcatc
     2881 tgaaaaccac tatgccattc agatagcaac agggaaagat gagaaggagc gtctcaacgc
     2941 tatttcacaa atggtctacg gcaagcgtgt agatgggcta atctttctct atgcccaaga
     3001 agaagaccct ctcgtaaaac tcgtcgcaga agaacagttc cccttcctta tcctaggtaa
     3061 atctctatct cctttcatcc cacttgtcga caacgacaat gttcaagctg gttttgatgc
     3121 gactgaatat ttcatcaaaa aaggctgcaa acgcattgcc tttatcggag gaagtaaaaa
     3181 gctcttcgtg accaaagacc gtttaacagg ctatgaacag gcgcttaaac attacaaact
     3241 taccactgac aacaatcgca tctactttgc cgacgagttt ctggaagaaa agggctataa
     3301 atttagcaag cgattattca agcacgatcc acaaattgat gctatcatca caaccgatag
     3361 cctcctagct gaaggtgttt gtaactatat tgccaaacac cagctggatg tccctgttct
     3421 cagctttgac tcggttaatc ccaagctcaa cttggcagcc tatgtcgata tcaatagttt
     3481 agagcttggt cgtgtttccc ttgaaactat tctccagatt attaatgata ataaaaacaa
     3541 taaacaaatt tgttaccgtc aattgatcgc ccacaaaatt atcgaaaaat aagagactgg
     3601 gcaaaaagtc gttaaaagca aaaacgcata ctatcaggta ttgaaaaaac ttgatactat
     3661 gcgttttatt gtgggaagat ttacttcctt ttctactgaa attgagtctt ttcccaagat
     3721 ctttttatac tcaatgaaaa tcaaagtgca aactaggaag ctagccgcag gttgctcaaa
     3781 acatggtttt gaggttgtag atgaaactga cgaagtcagt aaccatacct acggcaaggt
     3841 gaagctgacg tggtttgaag agattttcga agagtattaa tcactaatta tctatctcaa
     3901 caaatcttcc tagaatatga acattttccg agacagagac aaaggagctt ggatccactt
     3961 gtgtcataat ctgtttaaat tcattaaact ctgcacgtgt aatgacagtg attaaaactg
     4021 cctttctctc gtgattatag gttccttctg catcgtggat catggttgct ccgcggtgca
     4081 attttttatg gattttttca attaccttct ctggatgatt tgtcacaatc atggcctgca
     4141 tacgcttttg cttagtaaag actgcgtctg tcacacggct agagacaaag atggtaatca
     4201 tagaataaag agcgtatttc caaccaaagg ttaaacctgc tatcagcatg atagttccat
     4261 ttaccaagaa agaaatacta ccgacattct tacccgtttt cttgcgaata gtcaggctga
     4321 cgatatccgt cccaccactg gagatattgt ttcgaagagc aaaaccaatc cccaaaccca
     4381 taacaacacc cccaaaaagg gaattgataa tgggatcctc cgtcaaggtt gccacaggga
     4441 caaactggat aaagaaggaa ctcatagata ccgtgataaa ggtaaagacg gtgaacttat
     4501 ggccaatctg ataccaagct aagaccatca aagggaagtt aatggcgtag aagcttagcg
     4561 aaatcggaat atgaaaacca aaccagtgat tactcaaggc agagataatc tgtgccagac
     4621 ctgttgcacc actcgaatac acatgccctg gttggaaaaa gaaattaact gctactgctg
     4681 ataaaaaacc atagaccaga gaggccgaaa tcttctcatc atacttttct cgagagatac
     4741 tttgtaagac acgtaaaatt tttatctgat aagcaaagcg gcgcagataa tagcgccacc
     4801 gcttaattcg ttttgtttgt ttcatcttct tctacttgta agctgagttc ctctagttgt
     4861 ttgagagcga ctgttgatgg agcttgtgtc attgggtcag ttgccttgtt gttcttagga
     4921 aaggcaatga cttcacggat attttcttct ccagcaagca acatgacaaa acggtcaagc
     4981 ccgatagcca aaccaccgtg tggtgggaaa ccatagtcca tggcttcaag aaggaaacca
     5041 aactggtcat tggcttcttc agctgagaaa ccaagagcct tgaacatgcg ttcttgaagg
     5101 tctttttggt tgatacgaag gctaccacca ccaagctcat aaccgttcaa gacgatatcg
     5161 taagcaatgg cacgaacctt agccaaatca ccttctaatt cgtgagcagt ctcttcctgt
     5221 ggaagtgtga atggatggtg ggcgctcatg tagcggcctt cttcttcaga ccattcaaac
     5281 atcggccagt caaccaccca aaggaagttg aacttatcat tatcaatcaa gccaagctct
     5341 ttagcaatac gtccacgaag ggcacccagt gttgcattag ccacttcaag cgtatccgcc
     5401 acaaagagaa ccaagtcctt atcttcaaga gcaagcgctg ttgtcaattc ttcttggata
     5461 ccagtcaaga acttggcaac tggtccgttt aattctccat caaccacctt gacccaagca
     5521 agacctttgg caccatactg tttggctact tccgtcatct tgtcgatgtc tttacgtgaa
     5581 tagttgtccg cagctccttt gaccacaatc gcttttacag caagtgcttc tgaaaagact
     5641 ttaaagtcta cacctttgac cacttctgtc aagtcctgaa gcaacatgtc aaaacgagta
     5701 tctggcttgt cagaaccgta aagagccata gcatcatcgt atttcatacg agggaacggt
     5761 agcgttactt cgatgccttt tgtttccttc atcacgcgcg cgatcaagcc ttctgtaata
     5821 tcttggattt cttgctcagt aaggaaggac gtttccaagt cgacctgagt aaattcaggc
     5881 tggcggtctc cacgcaagtc ctcgtcacgg aaacatttaa cgatttggta gtaacggtca
     5941 aaaccagcat tcatcaagag ctgtttcgtg atttgtggac tttgaggaag agcgtaaaaa
     6001 tgccccttat taacacgaga cggcactaaa taatcacgcg ccccttcagg cgttgactta
     6061 gaaaggaatg gtgtctccac gtcgataaac tccaactcat ccaagtagtt gcggatagag
     6121 tgggtcacct tggcacgaag tttaagattt tccaacattt ctggacgacg aaggtcaagg
     6181 taacggtaac gcaaacgtgt atcgtcattt gcctcaatgc catccttaat ctcaaatggt
     6241 gttgtcttag ctgtgttaag cacaataaga gctgtcacgt ttaactcaac cgcaccagtt
     6301 ggcaacttat cattggcttg ctcacgcgca gcgacctgac cagtcacctc aataacaaat
     6361 tcgctacgaa ggctttcagc tgttgccata acctctgcag atactttttc agggttgata
     6421 accaactgca tgattccttc acggtcacga agatcgataa agatcaaacc accaaggtca
     6481 cgacgacggc caacccatcc tttcaaggtt atttcttgtc cgatgtgttc ctcacgaaca
     6541 cgaccagcat acatactacg tttcattatt tctctcctct tttattctgt tactatttta
     6601 ccataaaagc gcaggctctt catgaaaatc atcagaaaag tttgccagtc tttaaaagtc
     6661 aggtgaaagc cctaaaaatt agcgctaata ctcttcgaaa atctcttcaa accacgtcag
     6721 cgtcgcctta ccgtaggtat ggttactgac ttcgtcagtt tcatctacaa cctcaaaacc
     6781 atgttttgag ctgacttcgt cagtttcatc cacaacctca aaacagtgtt ttgagcaacc
     6841 tgcggctagc ttcctagttt gctctttgat tttcattgag tataatacaa aaatccgatg
     6901 aacttcaccg gactctttta ttttgaattt ttgcctgctt tacgcttttc agcgatttcg
     6961 gctgcctttc gaggcaagac aatttccgtt atgtaagccg tcccaaaacg cagtacacct
     7021 gcaataggag caaagacaac tgctagatag ttatagaaga aatcgccttt gaaggcataa
     7081 gctagcgctc caatgatgaa aaatagaacg actgcctgaa tcactgctaa taaaattact
     7141 cgtttcatgt gacctcctga ctctattata gcatgagaat catcaaaaag ccgactaaat
     7201 tattcaaagc gtgaagagaa atactgtaga ccagaccttt tctgctaacg taagccaaac
     7261 ccaaactaaa accaaggcta aaatagacaa aaaattgttg cacatcacct ggaaaatgaa
     7321 tcaaggcaaa tagaagacta gataccagaa gaaaaatcag ggttcgttta ctattgtcct
     7381 gcttaggaaa gagatagcgt gctaacatcc ctctaaaaac aatctcttcc gtcaaaggag
     7441 caaaaataac cacagcaaag aatgagaaaa gtggttgaga caaggtcaag tctgtcgcta
     7501 tttgctgatt tactgaagga tcatctggca agaagaattg aacgaccaga gataagaacc
     7561 aaaccaaggc aggaagccaa ataaatcggt taaagccgct cttctcaata taaacaggag
     7621 ctttctgata ccatttgtaa atgccgtaca catatactcc agccaagacc acatagagta
     7681 gagtaacagc atagggtgaa gcgcctaaag caagcgacgc agtcgcgagc ccctgaataa
     7741 agccatagat aaagaaaaag gatagaaggg ctagaagaat ccagccaagg tttttaagta
     7801 atttcataga taactccttt atttgaaata acgttttacc ataggtaact gcatcacatt
     7861 gatataaaca tggatggctc ctacaagcaa gaaagctagt aactgaatct ctcctgtcaa
     7921 gaaagaaatg ataataagaa aaatatataa ggctggtaag acatattggt gtaattggaa
     7981 taaaattcga aaactctgtt ccaaattagc ctgacgctcc ccttcatcat aagaatttat
     8041 atagttcaag acatcctttg gtgtagcgaa aaattccaaa tcaaactgac gaacaatcgc
     8101 aatggtttta aaaagagatt tttgagcgac taagaatacc acaaagagta agaaagaaag
     8161 gaaaaatgtt tgagggtttg tatgcaatat aatcacctca cttaatgaaa taaaaatagc
     8221 caatggaatc gctacacctg taatattaaa agcaatggtt ccaaactcaa gattccgata
     8281 catttgcaca taataggttt cattcagatc gtcatccatt tcctcttgat acaaggaatg
     8341 aaattttctg cttttcttta agaaattgaa agtcaaaaac atactaatga aacctatcag
     8401 taaacaaata gctgatatcc atggcatcaa ggcttttaca tctaaaataa tttcgtggga
     8461 ttcgacacgt gccttaaaca tccctacaaa catgcccaag aaccccccca agacaataga
     8521 catcaaaaat aacaatctac gtttcttttt catattcatt ctcctttttc acttgctaga
     8581 tttttggatt tcttttcaat ccattcagtt actgggatga gagcaaagta gacccaaaca
     8641 aattggtcgc tttgataggg attaaaccag cttaggtcca tcccaatcag tagaaatacg
     8701 ctgactaata aagctatgac cactacataa taaatcactt tatacttgtt catcactcgt
     8761 cctcctccaa acgaaatacc gattcgactg tttcgttgaa aatttgagat attttcaggg
     8821 caatgataat ggatggggtg tactcatccc gttctagtag gctaatggtc tgtctggaaa
     8881 cccctgccag tttggctagg tcggtttgat tgagaccatc gcgagctcga agctctttta
     8941 gacgattttt tagttgcacg ttacacacct actctccgtc aaattcaacg gtttggatat
     9001 cttcaatacg ttgcaacttg aatttttctt ttcccgtatt atcaacacta cgtagtttga
     9061 cccattcctc atcaacatcc acaacttccc agttatctgg cccaatatac actcccgtta
     9121 taattggttc ctttccaatc atttcttgta ataatctcga catttctgcg tttcctttct
     9181 cttttcgctc aagttttttg attttattct ctagtttctt gattttttta gaattattag
     9241 aataaaagaa aatcataaat agtataaatc ctagtaccca cattataact cctttctgct
     9301 tcctatttct taacttgaat tcattgtaac atatcttttt cttttttgac aattatagtt
     9361 gtcaaaaagt ttatgatttt tgtcattttg caaaagaaaa aggtcaggag taagttcctg
     9421 accactttat ctatcattaa tactcttcta aaatctcttc aaaccacgtc agcttcacct
     9481 tgccgtaggt atggttactg acttcgtcag tttcatctac aacctcaaaa ccatgttttg
     9541 agctgacttc gtcagttcta tccataactt caaaacagtg ttttgagcaa tctgcggcta
     9601 gcttcctagt ttgctctttg atttttattg agtataaaat cctagttttt caaagatttc
     9661 tgaaaagttt tggctgattg tctcaagtga cacttgcact tcttctcggg tttggttgtt
     9721 cttgaccgtc acttgtccgc tttcgacttc gctctctccc agggtgatga gggtcttagt
     9781 cgcaaagaca tcggctgact tgaactgagc tttgagctta cggttgaggt aatcacgctc
     9841 tgctttgaaa ccttgttggc gaagggcttg gactagttct agggctttga catttgctcc
     9901 atcacccaag actgcgatat agacatctag ggcattttcg atagggaggg ccacgccttg
     9961 tttttcaagg atgagaagca gacgctctac accaagccca aaaccaaatc cagcagtttc
    10021 agggcctcca aagtaagcaa ccaaaccatc gtagcgacca cccgcacaga ctgtcaagtc
    10081 attgccctca atctctgtga taaactcgaa aatggtgtgg ttgtagtagt ccagaccacg
    10141 caccatattg gtatcgatga tgtaatctac tccaagattt tccaacatct gacgcacagc
    10201 atcaaaatga gcttggcttt cttcatcaag aaagtccaag atagacggcg cattctctac
    10261 tgccaccttg tcttcttttt ccttagagtc caagacacga agaggatttt cctccaagcg
    10321 acgttggcta tccttagaca aggtctcctt gagcggtgtc aaatagtcaa tcaaggcttg
    10381 gcggtaggct gcacggctct caggatttcc aagagtgttg aggtgcaatt tgacaccttg
    10441 aataccgatt tccttcaaaa aatgggctgc cataacgatt gtttccacat cggtagctgg
    10501 attgctagag ccaaaacact caacaccaat ctggtggaat tggcgcaagc gccctgcctg
    10561 tggacgctca taacggaaca taggtcccat gtagtagaac ttgcttggct tttgcacttc
    10621 tggggcgaag agtttatttt ccacatagga acggacaacg ggcgcagttc cttctggacg
    10681 gagggtaata tggcggtcac ccttgtcata gaaatcgtac atttccttgg ttacgatatc
    10741 ggtcgtatct ccgacagagc ggctgataac ctcgtaatgc tcaaaaatgg gcgtgcgcac
    10801 ttctgcgtag ttgtagcgct tgaaaatttc acgggcaaag ccctcaacgt actgccattt
    10861 ggcagattca gcaggtaaaa tatcctgcgt tccttttggt ttttgtaatt tcatagggaa
    10921 tcctctttaa acttaatagt cttattttac cacaaataaa gggattaaaa cagtgagaaa
    10981 aaaattagga tttagatatc atttttgaga ttaagaattg tcaaaaaaat agctagcaag
    11041 gaaagaccaa caaatagcat ccaagtcaac tgtatattcc atacggctac tagtgaaaaa
    11101 caagctgttc ccacaggtat ggataaggta aacaatagac ctaaaaaatt actagtatga
    11161 gctagaacct ctggagctag atttttcatg agcatggcac taatctttgg ttgaacttta
    11221 ccagacacat acagagtaaa gaagagaaat agcaaaccaa gcacgacttg attgaataaa
    11281 ttagccaaac caactagact aagtcctacg gtcgcccaca tcatcaatct aggcaaggac
    11341 tgcttcccaa aataagcatt gcccgtaaga ctactgatga tgattgctac taaaacacag
    11401 aattgattga taaatagtgc ctctgtataa gaaaaattca agagagaatg gctcaaaaag
    11461 aagatattat aaattccacc caaagcgcca cccaaggaat taataagcaa gacagcaaag
    11521 agcataaaac caaagttttt ctgtccactt ttaagaaaaa cgagacgtaa atttcggtaa
    11581 attgttagga actggtcttt gatagaaagc ttctcatttt ttaagttttc accatcagca
    11641 gatgacattg acaggctcaa tttgcttttt cctaaaaaga ggatagtggc tgatactagg
    11701 aaaaagcagg cattgattcc cgcaacgaga gaaaaattgt tgaccgatag atctaagagc
    11761 cagactccga aagcttgacc accaatagct gaaatatagg tgatgaactg tgaaaaagaa
    11821 taagcctcca tcagatcatc ttcagctact ttttccttaa taagaggcat acgcaggcca
    11881 cctgcaaaat cactgatgat atcactaatg acattgatca aacacaggct agaaaaggca
    11941 aagagactag cttgctgaac aactagggct gctagaaaaa atagaaccgc ctgaaacaaa
    12001 ccgctataga ctatccattt gaccttgtcc ctcgtgtaat ctgcccgaat ccctgcaaaa
    12061 actgtaaaga aggtcggaag aatcatgaca atattcgcca tagcaacagc aaaagatgct
    12121 tgtgacaagg tcgatgcata gacgataaag accaggttga aaatcgaagc accaaaagca
    12181 ttgaagaagc gtgataaagt caaaattcta taagctggat ttctaaacaa tagtttcata
    12241 gataatctcc ctccttgttc taagttctac atcaagaata gctttagtat agcacttatg
    12301 aatttgcgaa acgattcttt tgaatatatg caacaaacaa aatgagcaaa aaaacgatag
    12361 aacttttgta atgacctgag agagggcaac agttctatcg cttcaaattt tttcttggtt
    12421 tgcagatact caacattcgg tcgtatttct atagtattcg gcagatttat tacagccaag
    12481 catctcaaaa atacggacag catcctctat ctttttctga ccttccttga ctctaccttg
    12541 cttgctatca aggagacctt ctgcccacag ataaacaatt cggaaatagg tctcattttc
    12601 cttgtagaaa tgctcttcga taacacgttt aaaataatag gcattggtaa attcttcaca
    12661 ctcaatacta gctaaaaagc cattcaatag tatagtatga aaaaggtttc gattgccaga
    12721 catttccatt agaaaatcga atttacgtac catttctcgt acatatctag taaaaagaga
    12781 aacggataaa aatggagaac tgactgaaaa taaattgagt tcatagattc cccagatctc
    12841 ggtagaaaac aaataatcat gaaggacttt tccttcctct gctgttaagt ctaccctttc
    12901 atctatgctc ttcatataag acttgatcat aatggcattt agaatatgtt tctgtttgtt
    12961 gtgagaatgg gcatgctttt gatactccct gcgatataag tcctcaagag gtgctatatt
    13021 ctttggttcc aagacatctg taatttcttt tctcaactca gaatctgtat catactggaa
    13081 acctctcgcc agaaagagga tctcctccac actggcagat atattttcca gagcaaatag
    13141 aaacttttcc accgaaagct cactctgacc tgtttcaaaa cgggacaaca tagacggcga
    13201 aaattgtcct ccggttgctt gtctcagtga gatatttctt gattctcgta attgtctaaa
    13261 gacttttcca atctgctcca tagacttccc cttgattccg tattttcttc attttatcat
    13321 atttttcaga aaattcatca aaaacttgcc aaattgtcag aattatgaga aaatagagga
    13381 tatttatcac gtggagggac tgctatgaga gacgatatca aaatcaatga ccgtgctttg
    13441 gccttgcaag accaaattat cgaaaaacta gagaaagttt ttgatacaga tgtggaattg
    13501 gatgtttaca atctaggtct gatttatgaa atcaatctgg atgaaacggg gctctgcaag
    13561 attgtcatga ccttcaccga tactgcctgt gattgcgccg aaagcctacc tattgaaatc
    13621 gtggcaggtc tgaaacaaat cgagggtatc aaagatatca aggttgaagt tacctggtcg
    13681 cctgcttgga aaatcacacg aatcagtcgc tatggccgta ttgcccttgg attaccacct
    13741 cgttaagcag gccaatcact tttaaagatg aaaaaagcaa gccgaaaatg gcttgctttt
    13801 tgatactcaa tgaaaatcaa agggcaaact agaaaactag ccgaaggttg ctcaaaacac
    13861 tgttttgaag tt
//
LOCUS       DAVLVP010000046        13797 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.046, whole genome shotgun sequence.
ACCESSION   DAVLVP010000046 DAVLVP010000000
VERSION     DAVLVP010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.046"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(148..519)
                     /locus_tag="VYN69_001290"
     CDS             complement(148..519)
                     /locus_tag="VYN69_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533556.1"
                     /translation="MIKKNHILCEIVPGSVAFACFYLSISISSVFQFLLVVINLTSIL
                     VTVYLTREMRLREFEDDDLVSPRTNQLMFIGLTGFMSIICLYRGITAGESYQQLIAYI
                     GAILCLIIMLLLIWGLKYYKK"
     gene            complement(991..1110)
                     /locus_tag="VYN69_001291"
     CDS             complement(991..1110)
                     /locus_tag="VYN69_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000346296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1533557.1"
                     /translation="MDFKSFIIGLVVGIFGPYMDDLIRKIFSKSSKKDTDSTL"
     gene            complement(1214..1363)
                     /gene="blpO"
                     /locus_tag="VYN69_001292"
     CDS             complement(1214..1363)
                     /gene="blpO"
                     /locus_tag="VYN69_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-like peptide BlpO"
                     /protein_id="HEV1533558.1"
                     /translation="MNTKMMSQFSVMDNEMLACVEGGDIDWGRKISCAAGVAYGAIDG
                     CATTV"
     gene            complement(1607..1810)
                     /gene="blpN"
                     /locus_tag="VYN69_001293"
     CDS             complement(1607..1810)
                     /gene="blpN"
                     /locus_tag="VYN69_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpN"
                     /protein_id="HEV1533559.1"
                     /translation="MNTYCNINETMLSEVYGGNSGGAAVVAALGCAAGGVKYGKILGP
                     WGAAIGGIGGAVVCGYLAYTATS"
     gene            complement(1826..2080)
                     /gene="blpM"
                     /locus_tag="VYN69_001294"
     CDS             complement(1826..2080)
                     /gene="blpM"
                     /locus_tag="VYN69_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-peptide bacteriocin subunit BlpM"
                     /protein_id="HEV1533560.1"
                     /translation="MNTKMMEQFHEMDIAMLSSIEGGKNNWQTNVFEGGSAAFGGWGL
                     GTAICAASGVGVPFMGACGYIGAKFGVALWAGVTGATGGF"
     gene            2355..4508
                     /gene="blpA"
                     /locus_tag="VYN69_001295"
                     /pseudo
     CDS             2355..4508
                     /gene="blpA"
                     /locus_tag="VYN69_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_125458604.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter BlpA"
     gene            4519..5880
                     /locus_tag="VYN69_001296"
     CDS             4519..5880
                     /locus_tag="VYN69_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin secretion accessory protein"
                     /protein_id="HEV1533561.1"
                     /translation="MNPNLFRSVEFYQRRYHNYATVLIIPLSLLFTFILIFSLVATKE
                     ITVTSQGEIAPTSVIASIQSTSDNPILANHLVANQVVEKGDLLIKYSETMEESQKTAL
                     ATQLQRLEKQKEGLGILKQSLEKATDLFSGEDEFGYHNTFMNFTKQSHDIELGITKTN
                     TEVSNQANLSNSSSSAIEQEITKVQQQIGEYQELRDAIINNRARLPTGNPHQSILNRY
                     LVASQGQTQGTAEEPFLSQINQSIAGLESSIASLKIQQAGIGSVATYDNSLATKIEVL
                     RTQFLQTASQQQLTVENQLTELKVQLDQATQHLGNNTLTAPSKGIVHLNSEFEGKNRI
                     PTGTEIAQIFPVITDTREVLITYYVSSDYLPLLDKGQTVRLKLEKIGNHGTTIIGQLQ
                     TIDQTPTRTEQGNLFKLTALAKLSNEDSKLIQYGLQGRVTSVTTKKTYFDYFKDKILT
                     HSD"
     gene            5937..6092
                     /gene="blpC"
                     /locus_tag="VYN69_001297"
     CDS             5937..6092
                     /gene="blpC"
                     /locus_tag="VYN69_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quorum-sensing system pheromone BlpC"
                     /protein_id="HEV1533562.1"
                     /translation="MDKKQNLTSFQELTTTELNQIIGGGWWEDFLYRFNIIEQKNTKG
                     FHQPIQL"
     gene            complement(6136..7476)
                     /locus_tag="VYN69_001298"
     CDS             complement(6136..7476)
                     /locus_tag="VYN69_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001099894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1533563.1"
                     /translation="MNVAWILLYTLVTHGLKIVIFFKVDGISLTFERIFKAFLFKILL
                     AVVFGMLGYMVGNVYLSYFMEPLYGIGLSFLLLRELPKKLLLFYGLFPMILVNLFYRG
                     VSYFVLPFLGQGQVYDDYSFIWLCIIIFNFFISLAFLKWLDYDFTSLRKGILDKDFQK
                     SLTQINWIMGAYYLVIQNLSYFEYEQGIQSTTVRHLILVFYLLFFMGIIKKLDTYLKD
                     KLHERLNQEQDLRYREMERYSRHIEELYKEVRSFRHDYTNLLTSLRLGIEEEDMEQIK
                     EIYDSVLKDSSEKLQDNKYDLGRLVNIRDKALKSLLAGKFIKAREKNIVFNVEVPEEI
                     QVEGMSLLDFLTVVSILCDNAIEASVEACQPHVSIAFLKNGAQETFIIENSIKEEGID
                     ISEIFSFGASSKGEERGVGLYTVMKIVESHPNTSLNTTCQDHVFRQVLTVIHIE"
     gene            complement(7490..8227)
                     /locus_tag="VYN69_001299"
     CDS             complement(7490..8227)
                     /locus_tag="VYN69_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001219153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1533564.1"
                     /translation="MKIFVLEDDFSQQTRIETTIEKLLKEHHITLSSFEVFGKPDQLL
                     AEVHEKGAHQLFFLDIEIRNEEMKGLEVARKIRDRDPYALIVFVTTHSEFMPLSFRYQ
                     VSALDYIDKALSAEEFESRIETALLYANGQDSKSLAEDCFYFKSKFAQFQYPFKEVYY
                     LETSTRPHRVILYTKTDRLEFTASLEEVLKQEPRLLQCHRSFLINPANVVHLDKKEKL
                     LFFPNGGSCLIARYKVREVSEAINNLH"
     gene            complement(8232..8570)
                     /locus_tag="VYN69_001300"
     CDS             complement(8232..8570)
                     /locus_tag="VYN69_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000682119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1533565.1"
                     /translation="MKHMIIQTQKTVYKVNIDDIYYIQTHPTKAHTVQIVTEKASFNM
                     LQNLSNLENQYGETLMRCHRNCLVNLDKVKSIDFQERILFLGEEGQYAVKYARRRYRE
                     IRQKWLKEGE"
     gene            8846..9175
                     /locus_tag="VYN69_001301"
     CDS             8846..9175
                     /locus_tag="VYN69_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase BlpT"
                     /protein_id="HEV1533566.1"
                     /translation="MTDTDPIKRAHTLITDLNKAYQACKQASADDVRFQEQLNSILGF
                     LAKAETVDNRFLIELEKFYQISSLLMGLSALDPDAPTRAAWRAYDRFHFDQVKTKLIL
                     NENQRAN"
     gene            complement(9343..10392)
                     /locus_tag="VYN69_001302"
     CDS             complement(9343..10392)
                     /locus_tag="VYN69_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1533567.1"
                     /translation="MKDLFLKRKQAFRKECLGYLRYVLNDHFVLFLLVLLGFLAYQYS
                     QLLQHFPENHWPILLFVGITSVLLLLWGGIATYMEAPDKLFLLVGEEEIKLHLKRQTG
                     ISLVFWLFVQTLFLLLFAPLFLAMGYGLPVFLLYVLLLGVGKYFHFRQKTSKFFTETG
                     LDWDYLISQESKRKQVLLRFFALFTQVKGISNSVKRRAYLDFILKAVQKVPGKIWQNL
                     YLRSYLRNGDLFALSLRLLLLSVLAQVFIEQAWIATAVVVLFNYLLLFQLLALYHAFD
                     YQYLTQLFPLDKGQKEKGLQEVVRGLTSFVLLVELVVGLITFQEKLALLALLGAGLVL
                     LVLYLPYQVKRQMQD"
     gene            complement(10389..11120)
                     /locus_tag="VYN69_001303"
     CDS             complement(10389..11120)
                     /locus_tag="VYN69_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000889926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533568.1"
                     /translation="MLEIKNLTGGYVHVPVLKDVSFTVESGQLVGLIGLNGAGKSTTI
                     NEIIGLLAPYSGSININGLTLQGDATSYRKQIGYIPETPSLYEELTLREHIETVAMAY
                     GIEQKVAFERVEPLLKMFRLEQKLDWFPVHFSKGMKQKVMIICAFVVDPSLFIVDEPF
                     LGLDPLAISDLIQLLEVEKQKGKSILMSTHVLDSAEKMCDAFVILHKGEVRAKGNLLQ
                     LREAFDMPEASLNDIYLALTKEEDL"
     gene            11188..11598
                     /locus_tag="VYN69_001304"
     CDS             11188..11598
                     /locus_tag="VYN69_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006150090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEV1533569.1"
                     /translation="MSDCIFCKIIAGEIPASKVYEDEQVLAFLDISQVTLGHTLVVPK
                     EHYRNLLEMDATSASQLFAQVPKVAQKVMKVTKAAGMNIISNCEEVAGQTVFHTHVHL
                     VPRYSADDDLKIDFIAHEPDFDKLAQVAETIKNA"
     gene            11608..11895
                     /locus_tag="VYN69_001305"
     CDS             11608..11895
                     /locus_tag="VYN69_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533570.1"
                     /translation="MKLSNLLLFAGAAAGSYLVTKNRQTITDEVLNTTDRVQAIKDDV
                     DIIQNSLQIINQQKELIKEYQEDLTYKFKVLEKDIQTRLAVIKEMQGTEDK"
     gene            complement(12320..13456)
                     /gene="dnaJ"
                     /locus_tag="VYN69_001306"
     CDS             complement(12320..13456)
                     /gene="dnaJ"
                     /locus_tag="VYN69_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEV1533571.1"
                     /translation="MNNTEFYDRLGVSKNASADEIKKAYRKLSKKYHPDINKEPGAED
                     KYKEVQEAYETLSDDQKRAAYDQYGAAGANGGFGGAGGFGGFNGAGGFGGFEDIFSSF
                     FGGGGSSRNPNAPRQGDDLQYRVNLTFEEAIFGTEKEVKYHREAGCRTCNGSGAKPGT
                     SPVTCGRCHGAGVINVDTQTPLGMMRRQVTCDVCHGRGKEIKYPCTTCHGTGHEKQAH
                     SVHVKIPAGVETGQQIRLAGQGEAGFNGGPYGDLYVVVSVEASDKFEREGTTIFYNLN
                     LNFVQAALGDTVDIPTVHGDVELVIPEGTQTGKKFRLRSKGAPSLRGGAVGDQYVTVN
                     VVTPTGLNDRQKVALKEFAAAGDLKVNPKKKGFFDHIKDAFDGE"
BASE COUNT         4526 a         2968 c         2344 g         3959 t
ORIGIN      
        1 cttatcaatc aagagcgatt tttaacataa tgtagcagca cccgttgcaa ctttgacaag
       61 tttagtatat cattgttttt taaaattttt catccaaatc ttgaattgtc atcgaaacct
      121 cctaaattgc aaaaacttca ttagccccta ctttttataa tacttcaaac cccaaatgag
      181 tagaagcatg atgatcaagc aaagaatagc accaatataa gcgattagtt gttgataaga
      241 ttctcctgct gtgatacctc tatacaaaca aataatagac ataaagcctg tcaagccgat
      301 aaacatgagt tgattggttc taggactaac caaatcatca tcttcaaact ctcttaatct
      361 catttcccta gtaagataaa cagtaaccaa aatagaagtc aagttaataa ctactaaaag
      421 aaattggaaa actgacgaaa tagaaataga taagtagaaa caagcaaagg caactgaccc
      481 aggaacaatc tcacaaagaa tatgattctt tttaatcaag aaaagttttc atttctttgc
      541 tcctttcttt ttagttaaag caaaatagat cataactgca atcacatagg ctatggtata
      601 aaatagctga taccaaatac tttccctaag cggataaaga aagatggaca tgattagata
      661 caagatgaaa atgataagta tttttttctt cataagattt cctcctaaat gtgtgcttta
      721 tcttagttga gcaagaacat ttacactgct agtatagcac ttattttgac tttggatcac
      781 tcaaatcata aatggtcatc aaaacctctt gaattgtaaa aattaaaaaa agcaagcatg
      841 aaaaacatac tttcctcttt atattgtatt gataccaact tgtttgtaga cttttcatcc
      901 tgctatcaca tatcattttg acaggcgaaa caatattaaa gaaactcccc tgtaaattaa
      961 gctagcaaat acaggggaga aatttatttt ttagagagta ctatccgtat cctttttgga
     1021 agattttgaa aatatttttc taattaagtc atccatataa ggaccaaata taccaactac
     1081 taaaccaata ataaaacttt taaaatccat aattaccacc aacatgttgc tacataggct
     1141 acacctccaa gtatagctcc acctgcagca ccagttacac ctattcctat agcaaatggt
     1201 cccaatagaa atgtcaaacc gttgttgcac acccatcaat tgcgccatat gcaacccctg
     1261 ctgcacaact aatttttctt ccccaatcaa tatctccacc ttcaacgcaa gcaagcattt
     1321 cattatccat aacagaaaat tgtgacatca tttttgtatt catatagtat gtcacttttc
     1381 agttacggaa caagtttaat ataaaaatta tcaaaaaaac ataggcaata aagagaaaaa
     1441 ttaatttatc atagattaga aataatatga caaaacaatt caatgatgtt aattcaatag
     1501 tcttttgttt tttatcagag atacttatgg atagataaat aagataggtt tgaaaagcga
     1561 agagaataat aaagaatata gccttcataa aatttagctt tcatttttat gatgtagcgg
     1621 tataggctaa atatccacaa accactgctc ctccaattcc tcctattgca gcgccccatg
     1681 gtcctagaat tttcccatat ttcactccac ccgctgcaca acctaaagca gcaactacag
     1741 ctgctcctcc ggaattacct ccgtaaacct cactcagcat tgtttcattt atattacaat
     1801 aagtattcat acaagtctcc ttttgttaaa atccacccgt tgcccctgtt actcctgccc
     1861 aaagagccac accaaattta gctcctatgt atccacatgc tcccataaat ggcactccaa
     1921 caccactcgc agcacaaata gctgtcccta atccccagcc accaaaagca gcactaccac
     1981 cttcgaagac attagtttgc caattattct tgcctccttc aatactagat aacatagcta
     2041 tatccatttc atgaaattgt tccatcattt ttgtattcat aacaaatact ccttttttat
     2101 ttttgtcttg ttgcaacttt gacaagttta gtatatcatt gttttttaaa atttttcatc
     2161 caaatcttga attgtcatcg aaacgtctta aattagcttt tttatttaaa actacctcta
     2221 aatttttttt aaaagataat ttctaatcac ttttttacca ttcaggaagt tttaatgact
     2281 attcaagatt tcataaaata tgaacttagt tttatggcat aatataccga tccactatat
     2341 gaaaggaatt gccaatgact tcttataaac gtacatttgt tcctcaaata gatgcgagag
     2401 actgtggtgt cgctgcctta gcctcgattg ctaaattcta tggttcagat ttttctctag
     2461 ctcacttgag agaacttgca aagaccaata aagaagggac gactgctctt ggcattgtaa
     2521 aagccgctga tgaaatgggc tttgaaacaa ggcctgttca agcagataaa acgctctttg
     2581 acatgagtga tgtcccctat ccatttatcg ttcacgttaa caaagaagga aaactccaac
     2641 attactatgt tgtctatcaa acaaagaaag actatctgat tattggtgat cctgaccctt
     2701 ctgtaaaaat cactaaaatg tcaaaagaac gctttttctc tgaatggact ggagtagcta
     2761 tttttctagc tcccaaaccc agctatcaac cccataaaga taaaaagaat ggtctactaa
     2821 gcttccttcc tctgattttc aaacaaaaat ctctcattgc ttacattgtt ctctcaagct
     2881 tattggtcac tattatcaat atcggtggtt cttattatct ccaaggaatc ttggatgaat
     2941 acatcccaaa tcagatgaaa tcaactttag gaatcatctc agttggtctg gttatcacct
     3001 atatcctcca acaagtcatg agcttttcca gagattatct cctaaccgtt ctgagccaga
     3061 gattaagcat tgatgtgatt ttatcctata ttcgccatat ttttgaactt cccatgtctt
     3121 tctttgcgac acgtcgtaca ggagaaatca tttcacggtt cacagatgct aactctatta
     3181 tagatgcctt ggcttctacc attctttctc tttttctgga tgtttctatt ctgattcttg
     3241 tagggggcgt cttactggca caaaacccta atctcttcct cctttctctt ctttccattc
     3301 ctatatacat gttcatcatc ttttctttta tgaaaccttt tgaaaaaatg aaccatgatg
     3361 tcatgcaaag taattctatg gttagctctg ccattatcga agatatcaac gggattgaaa
     3421 ctataaagtc gctcacgagt gaagaaaatc gctatcaaaa tatagacagc gaatttgtag
     3481 attatttgga aaaatccttt aagctcagta aatattctat tttacaaacg agtttaaagc
     3541 agggaacaaa attagttctg aatatcctta tcctatggtt tggcgctcaa ttagtcatgt
     3601 cgagtaaaat ttctatcggt cagctgatta cctttaacac acttttttct tactttacaa
     3661 ctcctatgga aaatattatc aacctccaaa ccaaactcca atctgcgaag gtcgctaata
     3721 accgtttgaa cgaagtctat ctagtcgaat ctgaatttca agttcaagaa aaccctgttc
     3781 attcacattt tttgatgggc gatattgaat ttgatgacct ttcttataag tatggttttg
     3841 gatgagatac cttaacagat attaatctca cgattaaaca aggagataag gttagcctag
     3901 ttggagttag tggttctggt aaaacaactt tagccaaaat gattgtcaat ttctttgaac
     3961 cctacaaagg gcatatttcc atcaatcatc aggatattaa caacattgat aaaaaagtct
     4021 tgcgccgtca tattaattac ctaccccaac aagcctatat ctttaatggc tctattttgg
     4081 aaaacttaac cttgggcggt aatcatatga ttagtcaaga agatattcta aaagcttgtg
     4141 aagtagctga aatccgtcaa gacattgaaa gaatgcctat gggctatcaa actcagctct
     4201 ctgatggagc tggtctatca ggaggacaga agcaacgaat cgctctcgct cgtgctcttt
     4261 taactaaatc tcctgtttta atactagatg aagctactag cggtcttgat gtcttgactg
     4321 agaaaaaggt tatagataat cttatgtctc taactgataa aaccattctc tttgtagccc
     4381 atcgtctcag tatagccgaa cgaaccaacc gtgtcattgt tcttgaccag gggaaaatca
     4441 ttgaagttgg tagtcaccaa gagttaatgc aggcgcaagg tttctaccat catctattca
     4501 ataaataagg agaatgtcat gaatcctaat ctttttagaa gcgtcgagtt ttatcagaga
     4561 cgttaccata actatgcgac agtgttaatt atacctcttt cattactatt tactttcatc
     4621 ttgattttct cccttgttgc cacaaaagaa attactgtta cttcccaagg agaaatcgcc
     4681 cctacaagtg tcattgcctc cattcagtca accagtgata atcctatcct agctaatcat
     4741 ttagtggcaa atcaagtagt tgaaaaaggg gacttactca tcaaatactc tgaaacaatg
     4801 gaagaaagtc agaaaactgc cttagcaact caattacaaa gacttgagaa gcaaaaagaa
     4861 ggacttggaa ttttgaaaca aagcttagaa aaagcgactg atcttttttc tggcgaggat
     4921 gaatttggct accataatac ctttatgaat tttactaaac aatcccatga tattgaactg
     4981 ggtatcacaa agactaacac cgaagtttca aatcaagcta atctttccaa tagcagttca
     5041 tcagctattg aacaagaaat tacaaaagtt caacaacaaa ttggagaata tcaagagttg
     5101 agagatgcta tcataaataa cagagcacgc ttaccaactg gcaatccgca ccagtcaatt
     5161 ttgaatcgtt atcttgtagc ctcacaagga caaacacaag gaactgcaga ggagccattt
     5221 ttatctcaaa ttaatcaaag tattgcaggt cttgaatcat ctatcgcaag cctcaaaatt
     5281 cagcaagctg gtatcggaag tgtagcaact tatgataaca gtttagcaac caaaattgaa
     5341 gtactccgca ctcagttttt acagacagcc tcacagcaac aactaactgt ggagaatcaa
     5401 ttaacagaat taaaagtaca actagatcaa gcaacgcagc acttaggaaa caatacctta
     5461 accgctccaa gtaaaggtat cgttcatctg aacagcgaat ttgaaggtaa aaatagaatt
     5521 ccaactggta cagaaattgc tcaaatattc cctgtcatca cagatacaag agaagtacta
     5581 atcacttact acgtatcttc tgactatcta cctctactag ataaaggaca aactgtaaga
     5641 ttaaaactgg agaagattgg aaatcacggc accaccatca tcggccaact tcagacaatt
     5701 gatcaaactc ctaccagaac agagcaagga aatctcttta aattaaccgc tcttgcaaaa
     5761 ctatctaacg aggatagtaa actcatccaa tatggcttac aaggtcgcgt cactagtgta
     5821 actacaaaga aaacatattt tgattatttc aaagataaaa ttttaacaca ttctgattaa
     5881 ttttcagata acactctata actatttatt atcttatcaa aaaggagaat cataacatgg
     5941 ataagaaaca aaacctaact tcatttcaag aactaacaac taccgaactc aaccaaatta
     6001 taggtggagg atggtgggaa gatttcttat atagatttaa tataattgaa caaaaaaata
     6061 caaaaggatt tcatcagcca atacaactat aaaaaaacaa gaccaagaga acgcactctc
     6121 ggtcttattt tttcttcatt ctatatgtat tacagtaagt acctgacgaa aaacgtgatc
     6181 ttggcaggtg gtattgagac tggtattggg atgactttcc acaattttca taacggtata
     6241 gagaccaact cctctctcct cccctttaga acttgctcca aaggagaaga tttcagaaat
     6301 atcgatgccc tcttctttga tggaattttc aatgataaag gtctcctgtg ctccattttt
     6361 taaaaaggca attgaaacat gaggttgaca ggcttctaca ctagcttcaa tagcgttgtc
     6421 acaaaggata gacacaacgg ttagaaaatc aagtagactc atcccctcaa cctgaatctc
     6481 ctcaggaact tcaacattaa agacaatgtt cttttctcta gcttttataa attttccagc
     6541 taggagactt ttgagggctt tatcacgaat attcaccaat cgaccaaggt catatttatt
     6601 gtcctgcaat ttttcactag aatcctttaa gaccgagtcg tagatctctt ttatctgctc
     6661 catatcctcc tcttcaatgc ccagacgtaa gctagtcaag aggttggtgt agtcatggcg
     6721 aaaactccgt acttccttat aaagctcctc tatatgccga ctataccgct ccatctctct
     6781 gtagcgcaag tcctgctctt ggttcagtct ctcatggagt ttgtccttca aataggtatc
     6841 caatttcttg ataatcccca taaaaaagag caggtaaaag actaggatga gatggcgaac
     6901 agtcgttgat tgaatcccct gttcatattc aaagtaagac agattttgta tcaccaaata
     6961 gtaagccccc attatccagt taatctgagt cagggacttt tgaaaatctt tatcgagaat
     7021 cccctttctc aagctagtga aatcatagtc caaccatttc aaaaaagcta gagaaatgaa
     7081 gaaattgaaa attattatac ataaccaaat aaatgagtag tcatcatata cttgcccttg
     7141 tcccaaaaat ggaagcacaa aataggaaac acctctataa aagagattca ccaatatcat
     7201 tggaaagaga ccataaaaga gaaggagttt tttaggaagt tctcttaaca ataagaaaga
     7261 taagcctatg ccgtacaagg gttccataaa ataagatagg taaacatttc ctaccatata
     7321 gccaagcatt ccaaaaacaa cggccaacag tatcttaaaa agaaaggcct taaaaatcct
     7381 ctcaaaagtg agactaattc catctacctt aaagaatatg acaattttta atccgtgagt
     7441 aacaagtgta tacaacaata tccaagcaac attcataaac tctcctagct cagtgtaagt
     7501 tattaatagc ctcagacact tccctgacct tataacgcgc gattagacaa cttccaccat
     7561 tgggaaagaa aagcagtttt tctttcttat ctaaatgcac cacattggca ggattgatga
     7621 gaaaagagcg atggcactgc aagagacgag gctcctgctt gagaacctcc tctagactcg
     7681 ctgtaaattc cagcctgtct gtcttggtat agagaataac acgatggggt cttgtggacg
     7741 tttcgagata gtaaacctct ttaaaaggat actggaattg ggcaaatttt gatttaaagt
     7801 aaaagcaatc ttccgctaga cttttactgt cttgaccatt ggcatagagg agggctgtct
     7861 cgatacgaga ttcaaactcc tctgccgaaa gggccttatc aatatagtcc agagcagaca
     7921 cttggtagcg aaaggacagg ggcataaact ccgagtgagt cgtcacaaag acaatcaggg
     7981 catacggatc ccgatcccga atctttctag ccacttccag acccttcatc tcctcatttc
     8041 gaatctcaat atccaaaaag aatagctgat gggccccctt ctcatgcacc tctgccagca
     8101 attgatctgg cttaccaaaa acttcaaaag aactcagagt gatatgatgt tccttcaaga
     8161 gtttctcaat cgtcgtttca attctagtct gttgggaaaa atcatcttct aaaacaaata
     8221 ttttcatctt cttactctcc ttctttcaac catttttgac gaatttctct atagcgacgt
     8281 ctggcatact tgacagcgta ttgaccttct tctccaagaa aaaggattct ttcttgaaaa
     8341 tcaatcgatt ttactttatc aagattgacc aaacaatttc gatgacatct catcaaggtt
     8401 tccccatatt ggttctcaag attacttaaa ttttgaagca tattaaaact agctttttct
     8461 gtaacaatct gtacggtatg ggctttagtt ggatgtgttt ggatatagta gatatcgtcg
     8521 atgtttactt tatagactgt tttctgtgtc tgaataatca tatgtttcat tggattctcc
     8581 tcctaagaaa agaataacag aaaaggtcta gcccaagccc acccaatcct aaacagtctt
     8641 tgaaaactcc taaaaggatt attttccaca acatcatgat gagcttacaa ctattttatc
     8701 ataaaattca ctcagcgcaa ttcaagacat ttcaatgaca attaaagatt tgaataaaaa
     8761 aaaagaatta aaagtgataa actaacagta tcaaaacaca gttgccagaa gtaagactgg
     8821 cacccagatt aaaggaggca ttcccatgac agatacagac cccatcaaaa gagctcatac
     8881 tttgattact gacttaaaca aagcctacca agcatgcaaa caggcaagcg ctgacgacgt
     8941 ccgctttcag gagcaattaa actctattct tggttttcta gccaaggctg aaacagtgga
     9001 taatcgattc ttgattgaac tagaaaaatt ttaccagatt tccagtcttc tcatgggact
     9061 cagcgctctt gatccagatg ctccaactcg cgccgcttgg cgggcctatg accgcttcca
     9121 ctttgaccaa gtcaagacca agttaatact caatgaaaat caaagagcaa actaggaagc
     9181 tagccgcaag ctgtacttga gtacggtaag gcgacgctga cgtggtttga agagattttc
     9241 gaagagtata agtctctacg gaccaaccat tatcctgtag taacaaaaaa gaagctgaga
     9301 cagactgaac tcaacttctt ttttagtgtc gtatcagcaa tgttagtcct gcatctgacg
     9361 ttttacctga taaggcaaat acaagactag taaaaccaaa ccagctccta gtaaggctag
     9421 aagggccagt ttttcttgaa aggtaatcaa cccaacaact aattccacaa gtaaaacaaa
     9481 actggtcaat cctcgaacta cctcctgtaa gcctttttcc ttttgcccct tgtccagcgg
     9541 aaagagttgg gtcaaatact ggtagtcaaa ggcatgatag agggccagca actggaagag
     9601 caagaggtag ttaaagagaa ctaccactgc tgtcgcaatc caagcttgct cgataaaaac
     9661 ctgcgccagt acggaaagta atagaagacg aagactgaga gcaaagaggt cgccatttcg
     9721 cagataagaa cgcagataga gattttgcca aatcttccca ggcaccttct gaacagcctt
     9781 taagataaag tccagatagg cacgacgctt aacgctgttt gaaattccct tgacctgcgt
     9841 aaagagggca aagaaacgaa gcaagacttg cttacgcttg ctttcttgag aaataagata
     9901 gtcccagtcc agtccagttt cagtgaaaaa tttgctggtc ttttgacgaa agtggaaata
     9961 ctttcctacc cccaataaaa gcacatagag cagaaaaact ggcaagccat aacccattgc
    10021 taaaaataaa ggcgcaaata acagcaagaa aagggtctgt acaaagagcc aaaagactag
    10081 ggaaatgcca gtttgacgct tgagatggag cttaatttcc tcttctccaa ctaagagaaa
    10141 gagcttgtct ggagcctcca tataggtggc aattcctccc caaagtaaaa gtaaaacaga
    10201 cgtaattcct acaaacaaaa ggataggcca atgattttca ggaaaatgtt gtaagagttg
    10261 actgtactgg taggctagaa agcccaacag gacaagcagg aacaagacaa agtggtcatt
    10321 gagcacatag cgcagataac caagacactc cttacgaaag gcctgctttc tctttaaaaa
    10381 caagtctttc atagatcctc ctctttggtc agagccaagt aaatatcatt caaactagcc
    10441 tcaggcatat caaaggcttc acgtagttgc aggagattgc ctttggcacg cacctctccc
    10501 ttgtgaagaa tgacaaaggc atcacacatc ttctccgccg aatccagcac atgggtactc
    10561 atgagaatag acttgccctt ttgcttctcc acttccaaaa gctgaatcaa atcagaaata
    10621 gccagcggat caagaccaag gaaaggctca tccacgatga aaagacttgg atccaccaca
    10681 aaagcacaga taatcatgac cttctgcttc atcccttttg aaaaatgaac agggaaccag
    10741 tctaatttct gttccaaacg gaacattttt aacaagggct ctactcgttc gaaagccact
    10801 ttttgctcaa taccgtaagc catagcaacc gtttcgatat gctctctgag ggtcaattcc
    10861 tcatacagac taggcgtctc aggaatgtag ccaatctgct tgcggtagct agtcgcatct
    10921 ccttgcagag tcaggccatt gatattgatg gagccactat aaggtgccaa cagaccgata
    10981 atctcattga tcgtcgttga tttcccagca ccattgagac caatcaaacc gaccagctgc
    11041 ccactttcaa cagtaaagga cacatctttc aaaacaggaa catgaacata gccacctgtc
    11101 aggtttttaa tttctaacat attttctcca aatctggtat aatgtagcta tattatatca
    11161 aaattcagta cagtagaggt agattttatg tcagattgca ttttttgtaa aatcatcgca
    11221 ggggaaattc ctgcttcaaa agtatatgaa gatgagcagg tccttgcctt tcttgatatc
    11281 tctcaagtaa cactaggaca caccttggtc gtgccaaaag aacactatcg caatcttttg
    11341 gagatggatg ctacgagcgc cagccaactc tttgcccaag taccaaaagt agctcaaaaa
    11401 gtcatgaaag tcactaaggc tgctggtatg aatatcattt ccaactgtga agaagtcgct
    11461 ggtcaaacag tttttcatac tcacgttcac cttgtgcctc gctacagtgc tgacgatgac
    11521 ctcaagattg attttatcgc ccacgaacca gactttgaca aacttgctca agtcgctgaa
    11581 accatcaaaa acgcttaagg agttgctatg aaattatcca acctactgct atttgcagga
    11641 gctgcagccg gaagttatct ggttacaaaa aatcgccaaa ccatcacaga tgaagtcttg
    11701 aataccactg accgcgttca agctatcaag gacgatgtgg atattatcca aaacagcctg
    11761 caaatcatta accagcaaaa agaacttatc aaggaatacc aagaagactt gacttacaag
    11821 tttaaggtct tggaaaagga tatccagact agactagctg tgataaaaga aatgcaggga
    11881 actgaagata agtaaaaaga gcccgacggc tcttttttaa aacgtaaaca agttatagtg
    11941 aattgaattt agaatagcac atcatagttt ctaaagcatt gttagagatt actttaaaat
    12001 cctcttatca atttgttcat attctatttc aatctactat atatatccca tcaactatgc
    12061 atcataattt aggtaactca tactcaataa aaatcaaaaa gcaaactaga aagctaggca
    12121 cagactgctc aaaacaccgt tttaaggttg tggatagaac tgacgaagtc aactcaaaac
    12181 actgttttga ggttgtggat agaactgacg aagtcagctc aaaacactgt tttgaggttg
    12241 tggatagaac tgacgaagtc agtaaccata cccatggcaa gccgacgctg acgtggtttg
    12301 aagagatttt cgaagagtat tattctccat caaaggcatc ttttatatgg tcaaagaagc
    12361 cttttttctt tggatttact ttcaagtcac cagcagccgc gaattctttc aaggctactt
    12421 tttggcggtc gttcaagcct gtcggtgtta cgacattaac agtaacgtat tggtcaccaa
    12481 ctgcaccgcc acgaaggctc ggtgccccct tactacgtag gcggaatttc ttaccagtct
    12541 gagttccctc tggaataacc aattcaacat caccgtgaac agttggaata tctactgtat
    12601 caccaagagc cgcttggaca aagttgaggt tgagattgta gaagatagtc gttccttcac
    12661 gttcaaactt gtcgctagct tctacagaaa ctactacata caagtcccca taaggtccac
    12721 cgttaaagcc tgcttcgcct tgaccagcga ggcgaatttg ttgacctgtt tccacaccag
    12781 cagggatttt cacatgtacg ctatgagctt gtttctcatg acctgttcca tgacaggttg
    12841 tacatggata tttgatttct tttcctcgac cgtgacagac atcacaggtt acttggcgac
    12901 gcatcatacc aagaggagtc tgcgtatcga cgttaatgac accagcgcca tgacagcgtc
    12961 cacaagtgac tggacttgtc cctggcttag caccagagcc attacatgta cgacagccag
    13021 cttcacgatg atacttaact tccttctcag ttccgaagat agcttcttca aaggtcaaat
    13081 tgacacgata ctggagatca tctccttggc gaggagcgtt tggattgcgc gaagaaccgc
    13141 ctccgccgaa gaaacttgag aaaatatcct caaaaccacc gaagccacct gccccattga
    13201 aaccgccgaa accaccagct ccaccaaaac caccattggc gcctgcagca ccatactggt
    13261 cataggcagc acgtttttgg tcgtcactca aagtctcata ggcttcttga acttccttgt
    13321 acttgtcctc agcaccaggc tccttgttga tatctgggtg atattttttg gaaagcttac
    13381 gataagcctt tttgatttcg tctgccgaag cgtttttgga tacccccaga cgatcataaa
    13441 attcagtatt gttcataatt caagatacaa agggcgtcaa acggacacag ccatctagga
    13501 gttttgacga cggacgctta tgtcctagaa aaaactatct ttttggcaca gtccgtagcc
    13561 cgtgttcagt tacgaacacc cttattcctt tctattgatg ttctgaatcg aaccaccatt
    13621 taggtcggat tcaattcctt tcttttatat ttagtaagaa attctgaaac ccgtgttcag
    13681 ttacgaacac tctttgttcc tttctattga tgagacaaaa cccaaacaaa taagattccg
    13741 tcccttttta tcgaaaaaca gaggctgggt tgcaacctct gcaaataagt aaattaa
//
LOCUS       DAVLVP010000047        13794 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.047, whole genome shotgun sequence.
ACCESSION   DAVLVP010000047 DAVLVP010000000
VERSION     DAVLVP010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13794)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13794)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13794
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.047"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(146..1777)
                     /locus_tag="VYN69_001307"
     CDS             complement(146..1777)
                     /locus_tag="VYN69_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004251733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HEV1533572.1"
                     /translation="MSINWQEILFHFLGGLGLFLYSIKTMGDGLQQAAGDRLRFYIDK
                     YTSNPFFGVLVGIGMTALIQSSSGVTVITVGLVSAGLLTLRQAIGIVMGANIGTTVTS
                     FLIGFKLGNYALPMLFIGAVCLFFTKNRTVNNIGRILFGVGGIFFALNLMSGAMAPLK
                     DLQVFKDYMIELSKNPVLGVFVGTGLTLLIQASSATIGILQNLYAGNLIDLQGALPVL
                     FGDNIGTTITAIIASLGANIAAKRVAGAHVAFNVIGTVVCVIFLVPFTVLIHWFEATL
                     NLAPEMTIAFAHGTFNITNTIVQFPFIGALAYFVTKIIPGEDEVVKYEPLYLDEHFIK
                     QAPSIALGNAKKELLHLGNYAAKAFDLSYKYIIDLDEKVAEKGHKTEEAINTIDEQLT
                     RYLIALSSEALSQKESEVLTNILDSSRDLERIGDHTEALLNLTDYLQRKNVEFSDAAL
                     KELEEVYRQTSDFIKDALDSVENNDIEKARSLVERHEAINKIERVLRKTHIKRLNKGE
                     CSTQAGVNFIDIISHYTRVSDHAMNLAEKVFAEQI"
     gene            2070..7049
                     /locus_tag="VYN69_001308"
     CDS             2070..7049
                     /locus_tag="VYN69_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000795197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEV1533573.1"
                     /translation="MKNPFFERRCRYSIRKLSVGACSLMIGAVLFAGPALAEETAVPE
                     NSGANTELVSGESEHSTNEADKQNEGEHARENKLEKAEGVAIASETASPASNEAATTE
                     TAEAASAAKPEEKASEVVAETPSAEAKPKSDKETEAKPEATNQGDESKPAEEANKTEK
                     EVQPDVPKNTEKTLKPKEIKFNSWEELLKWEPGAREDDAINRGSVVLASRRTGHLVNE
                     KASKEAKVQALSNTNSKAKDHASVGGEEFKAYAFDYWQYLDSMVFWEGLVPTPDVIDA
                     GHRNGVPVYGTLFFNWSNSIADQERFAEALKQDADGSFPIARKLVDMAKYYDYDGYFI
                     NQETTGDLVKPLGEKMRQFMLYSKEYAAKVNHPIKYSWYDAMTYNYGRYHQDGLGEYN
                     YQFMQPEGDKVPADNFFANFNWDKAKNDYTIATANWIGRNPYDVFAGLELQQGGSYKT
                     KVKWNDILDENGKLRLSLGLFAPDTITSLGKTGEDYHKNEDIFFTGYQGDPTGQKPGD
                     KDWYGIANLVADRTPAVGNTFTTSFNTGHGKKWFVDGKVSKDSEWNYRSVSGVLPTWR
                     WWQTSTGEKLRAEYDFTDAYNGGNSLKFSGDVAGKTDQDVRLYSTKLEVTEKTKLRVA
                     HKGGKGSKVYMAFSTTPDYKFDDADAWKELTLSDNWTNEEFDLSSLAGKTIYAVKLFF
                     EHEGAVKDYQFNLGQLTISDNHQEPQSPTSFSVVKQSLKNAQEAEAVVQFKGNKDADF
                     YEVYEKDGDSWKLLTGSSSTTIYLPKVSRSASAQGTTQELKVVAVGKNGVRSEAATTT
                     FDWGMTVKDTSLPKPLAENIVPGATVIDSTFPKTEGGEGIEGMLNGTITSLSDKWSSA
                     QLSGSVDIRLTKQRTVVRWVMDHAGAGGESVNDGLMNTKDFDLYYKDADGKWKLAKEV
                     RGNKAHVTDITLDKPITAQDWRLNVVTSDNGTPWKAIRIYNWKMYEKLDTESVNIPMA
                     KAAARSLGNNKVQVGFADVPAGATITVYDNPNSQIPFATLKSEVGGDLASAPLDLTNQ
                     SGLLYYRTQLPGKEISNVLAVSVPKDDRRIKSVSLETGPKKTSYAEGEDLDLRGGVLR
                     VQYEGGTEDELIRLTHAGVSVSGFDTHHKGEQNLTLQYLGQPVNANLSVTVTGQDEAS
                     PKTILGIEVSQEPKKDYLVGDSLDLSEGRFAVAYSNDTMEEHSFTDEGVEISGYDAQK
                     TGRQTLTLHYQGHEVSFDVLVSPKAALNDEYLKQKLAEVEAAKNKVVYNFASPEVKEA
                     FLKAIEAAEQVLKDHEISTQDQVNDRLNKLTEAHKALNGQEKFKEEKTELDRLTGEVQ
                     ELLAAKPNHPSGSALAPLLEKNKALVEKVDLSPEELATAKQSLKDLVALLKEDKPAVF
                     SDSKTGVEVHFSNKEKTVIKGLKVERVQASAEEKKYFAGEDAHVFEIEGLDEKGQDVD
                     LSYASIVKIPIEKDKKVKKVFFLPEGKEAVELAFEQTDSHVIFTAPHFTHYAFVYESA
                     EKPQPAKPAPQNTVLPKPTYQPASDQQKAPKLEVQEEKVAFHRQEHENAEMLVGEQRV
                     IIQGRDGLLRHVFEVDEKGQRRLRSTEVIQEAIPEIVEIGTKVKTVPAVVATQEKPAQ
                     NTAVKSEEASKQLPNTGTADANETLIAGLASLGLASLALTLRRKREDKD"
     gene            7139..8335
                     /locus_tag="VYN69_001309"
     CDS             7139..8335
                     /locus_tag="VYN69_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEV1533574.1"
                     /translation="MAKLTVKDVDLKGKKVLVRVDFNVPLKDGVITNDNRITAALPTI
                     KCIIEQGGRAILFSHLGRVKEEADKAGKSLAPVAADLAAKLGQDVVFPGVTRGAELEA
                     AINALEDGQVLLVENTRYEDVDGKKESKNDPELGKYWASLGDGIFVNDAFGTAHRAHA
                     SNVGISANVEKAVAGFLLENEIAYIQEAVETPERPFVAILGGSKVSDKIGVIENLLEK
                     ADKVLIGGGMTYTFYKAQGIEIGNSLVEEDKLDVAKALLEKANGKLILPVDSKEANAF
                     AGYTEVRDTEGEAVSEGFLGLDIGPKSIAKFDEALTGAKTVVWNGPMGVFENPDFQAG
                     TIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKVLPGL
                     AALTEK"
     gene            8471..8998
                     /locus_tag="VYN69_001310"
     CDS             8471..8998
                     /locus_tag="VYN69_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEV1533575.1"
                     /translation="MSYFKKYKFDKSQFKLGMRTFKTGIAVFLVLLIFGFFGWKGLQI
                     GALTAVFSLRESFDESVHFGTSRILGNSIGGLYALVFFLLNTFFHEAFWVTLVVVPIC
                     TMLTIMTNVAMNNKAGVIGGVAAMLIITLSIPSGETILYVFVRVLETFMGVFVAIIVN
                     YDIDRIRLFLEKKEK"
     gene            9075..9431
                     /gene="glnR"
                     /locus_tag="VYN69_001311"
     CDS             9075..9431
                     /gene="glnR"
                     /locus_tag="VYN69_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000659547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor GlnR"
                     /protein_id="HEV1533576.1"
                     /translation="MKEKEFRRNMAVFPIGSVMKLTDLSARQIRYYEDQELIKPDRNE
                     GNRRMYSLNDMDRLLEIKDYISEGYNIAAIKKKYAEREAKSKKAVSQTEVRRALHNEL
                     LQQGRFASVQSPFGRG"
     gene            9468..10814
                     /gene="glnA"
                     /locus_tag="VYN69_001312"
     CDS             9468..10814
                     /gene="glnA"
                     /locus_tag="VYN69_001312"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEV1533577.1"
                     /translation="MPITAADIRREVKEKNVTFIRLMFSDILGTMKNVEIPATDEQLD
                     KVLSNKVMFDGSSIEGFVRINESDMYLYPDLDTWTVFPWGDENGSVAGLICDVYTTEG
                     EPFAGDPRGNLKRALRHMEEVGFKSFNLGPEPEFFLFKLDENGDPTLEVNDKGGYFDL
                     APTDLADNTRREIVNVLTKMGFEVEASHHEVAVGQHEIDFKYDEVLRACDKIQIFKLV
                     VKTIARKHGLYATFMAKPKFGIAGSGMHCNMSLFDAEGNNAFFDPNDPKGMQLSETAY
                     HFLGGLIKHAYNYTAIMNPTVNSYKRLVPGYEAPVYIAWAGRNRSPLVRVPASRGMGT
                     RLELRSVDPMANPYVAMAVLLEVGLYGIENKIEAPAPIEENIYIMTAEERKEAGITDL
                     PSTLHNALKALTEDEVVKAALGDHIYTSFLEAKRIEWASYATFVSQWEIDNYLDLY"
     gene            complement(10910..11029)
                     /locus_tag="VYN69_001313"
     CDS             complement(10910..11029)
                     /locus_tag="VYN69_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase"
                     /protein_id="HEV1533578.1"
                     /translation="MPDIVFEIEFFHSDSLIFFYTSDDKSNSQKSQEDFSKNK"
     gene            11574..12893
                     /locus_tag="VYN69_001314"
     CDS             11574..12893
                     /locus_tag="VYN69_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1533579.1"
                     /translation="MPDTWEWVRFSTLVEIVRGGSPRPIKDYLTSEVDGINWIKIGDT
                     EKGEKYINNVKEKIKKSGLNKTRFVKKGTFLLTNSMSFGRPYILNVDGAIHDGWLAIS
                     NYENSLNKDYLFYILSSNVVYSQFLSLISGAVVKNLNSDKVASILIPLPPLSEQQRIV
                     EAIESALEKVDEYAESYNRLEQLDKEFPDKLKKSILQYAMQGKLVEQDPNDESVEVLL
                     EKIRAEKQKLFEEGKIKKKDLDISIVSQGDDNSYYEEVPCEIPESWEWVRLNDITSYI
                     QRGKSPKYSNIPIYPVIAQKCNQWSGFSIDLARFIDPETVHSYQKERLLRDGDLMWNS
                     TGLGTLGRLAIYHENKNPYAWAVADSHVTVIRVLSGVINCHFIYNFLSSPIVQSVIEE
                     KASGSTKQKELLTKTIKEYLIPLPPLPEQSRIVDKIEQFFAHIDALI"
     gene            12950..13747
                     /gene="psrA"
                     /locus_tag="VYN69_001315"
     CDS             12950..13747
                     /gene="psrA"
                     /locus_tag="VYN69_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000651177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type DNA invertase PsrA"
                     /protein_id="HEV1533580.1"
                     /translation="MKDFELHLKKAGLAENTVRSYLYGVRFFLENYELKMEDLFEYKG
                     YLLDNFKPKTVNLRLQGVNKYLAFIGHDDLKLKFVKVQQKPFLEDVISHADYLFLKRS
                     LKKDGILKWHFVVWFLGATGARVSELIKLKVEHVEIGYFDIYSKGGKIRRLYIPKKLR
                     NSCLSWLESENRRSGYLFLNKFNEPITARGVAQQLKNYADKYKMNPKVIYPHSFRHLF
                     AKNFLAKYNDIALLADLMGHESIETTRIYLRKTATEQQNIVDKIVNW"
BASE COUNT         4496 a         2431 c         2912 g         3955 t
ORIGIN      
        1 ctaacaatgt tttagaaata gaggtgtact attctagttt caatatacta tatatctgca
       61 gaatactctt tcagtttcat tcagcctagt cttgcttagg aaaaagtaaa aagccatcca
      121 attatgatgg atagcttctt ggttcttaga tttgttctgc aaaaaccttt tcagcaaggt
      181 tcatagcgtg gtctgataca cgagtgtagt gtgagatgat gtcgataaag ttgaccccag
      241 cttgtgttga acattcgcct ttgttgaggc gtttgatgtg ggtttttctg agaacacgtt
      301 ctatcttatt gattgcttca tgacgttcta caagactgcg tgctttttca atatcattgt
      361 tttccacact atccagagca tctttgataa agtcactagt ttggcggtaa acttcctcta
      421 attctttcaa ggcggcatca gaaaattcaa catttttccg ttgaagatag tcagtcagat
      481 tgagtagagc ctccgtgtgg tctccaatcc gttccaaatc acgggaggaa tcaaggatat
      541 tggtaagcac ttcactttct ttttggctga gagcttcgct tgaaagggca atgagataac
      601 gtgttaattg ctcatcgatg gtgttaattg cttcttcggt tttatgccct ttttcagcaa
      661 ctttttcatc caagtcaatg atgtacttat aggaaaggtc aaaggcttta gcagcgtagt
      721 ttcctaagtg caagagctct ttcttagcat ttcctagagc gatagatggg gcctgtttga
      781 tgaaatgttc atcaagatat aagggttcgt atttgacaac ctcgtcctct ccaggaataa
      841 tcttggttac aaagtaagcc agagctccga taaatggaaa ttggacaatg gtgttggtaa
      901 tattaaaggt tccgtgagca aaggcgatgg tcatttccgg tgctagattt agcgtagctt
      961 caaaccaatg aatcaggaca gtaaaaggaa ctagaaaaat aacgcagacg actgttccga
     1021 taacgttgaa ggcaacatga gctcctgcta cccgtttagc tgcaatatta gcccctaaag
     1081 aggcaatgat tgctgtaatg gttgtcccga tattgtcacc aaatagaacc ggcaaagctc
     1141 cctgtaggtc aattagattg ccggcgtaga ggttttgtaa aatcccaatg gtagccgaag
     1201 aagcttgaat tagcaaggtc aagccagtac cgacaaagac acccaaaaca ggatttttac
     1261 ttagctcaat catatagtcc ttaaagacct gtaaatcctt gagtggagcc attgcgccgc
     1321 tcatgagatt gagggcaaaa aagataccac cgacaccaaa gaggatgcgt ccgatattat
     1381 tgactgtccg attttttgta aaaaagagac agacggcacc gataaagagc ataggtaggg
     1441 catagttacc taatttaaaa ccgatgagaa aggatgtgac agttgtccca atattagcac
     1501 ccatgacaat cccgatagcc tgacgtaagg ttaagagacc ggcactgacc aggccgactg
     1561 tgataactgt tacaccagaa ctagactgaa ttagagcagt catcccaata ccaaccagaa
     1621 ctccaaagaa aggattacta gtatatttgt caatgtaaaa acgaaggcga tctccagcag
     1681 cttgttgtaa accgtctccc atggtcttga tgctatataa gaatagcccc agaccaccta
     1741 aaaagtgaaa taaaatttcc tgccaattaa tggacatttc tttttcctcc gaaaaataat
     1801 cgcggaatat ctcctattct attttaaagg ataaaagtaa atctaacaag tgttaatgtc
     1861 aagtttgtaa aagaaaatta taattgataa gtgtattatt tttaagagtt tttaacctta
     1921 ttttagattt gtgcaaacag ttattgtttt ttgtctaaaa atgttttagg ataaaaatgt
     1981 gattatcatg gttctagagt ttgataaaaa accacagaat ataaagcgct ttcttgaaaa
     2041 caacaaaatc aatcttttag gaggagaaaa tgaagaatcc attttttgaa agacgttgtc
     2101 gttacagtat tcgtaagtta tcagtaggag cctgctcgct gatgattggt gctgttttat
     2161 ttgctggtcc agccttggct gaagaaactg cagttcctga aaatagcgga gctaatacag
     2221 agcttgtttc aggagagagt gagcattcga ccaatgaagc tgataagcag aatgaagggg
     2281 aacatgctag agaaaacaag ctagaaaagg cagaaggagt agcgatagca tctgaaactg
     2341 cttcgccagc aagcaatgaa gctgcaacta ctgaaactgc agaagcagct agcgcagcta
     2401 aaccagagga aaaagcaagt gaggtggttg cagaaacacc atctgcagaa gcaaaaccta
     2461 agtctgacaa ggaaacagaa gcaaagcccg aagcaactaa ccaaggggat gagtctaaac
     2521 cagcagaaga agctaataag actgaaaaag aagtccagcc agatgtccct aaaaatacag
     2581 aaaaaacatt aaaaccaaag gaaatcaaat ttaattcttg ggaagaattg ttaaaatggg
     2641 aaccaggtgc tcgtgaagat gatgctatta accgcggatc tgttgtcctc gcttcacgtc
     2701 ggacaggtca tttagtcaat gaaaaagcta gcaaggaagc aaaagttcaa gccttatcaa
     2761 acactaattc taaagcaaaa gaccatgctt ctgttggtgg agaagagttc aaggcctatg
     2821 cttttgacta ttggcaatat ctagattcaa tggtcttctg ggaaggtctc gtaccaactc
     2881 ctgacgttat tgatgcaggt caccgtaacg gggttcctgt atacggtaca ctcttcttca
     2941 actggtctaa tagtattgca gatcaagaaa gatttgctga agctttgaag caagacgcag
     3001 atggtagctt cccaattgct cgtaaattgg tagacatggc aaagtattat gactatgatg
     3061 gctatttcat caaccaagaa acaactggag atttggttaa acctcttgga gaaaagatgc
     3121 gccagtttat gctctatagc aaggaatatg ctgctaaggt aaaccatcca atcaagtatt
     3181 cttggtatga tgccatgacc tataactatg gacgttacca tcaagatggt ttgggagaat
     3241 acaactacca attcatgcaa ccagaaggag ataaggttcc ggcagataac ttctttgcta
     3301 actttaactg ggataaggct aaaaatgatt acactattgc aactgccaac tggattggtc
     3361 gtaatcctta tgatgtattt gcaggtttgg aattgcaaca gggtggttcc tacaagacaa
     3421 aggttaagtg gaatgacatt ttagacgaaa atgggaaatt gcgcctttct cttggtttat
     3481 ttgccccaga taccattaca agtttaggaa aaactggtga agattatcat aaaaatgaag
     3541 atatcttctt tacaggttat caaggagacc ctactggcca aaaaccaggt gacaaagatt
     3601 ggtatggtat tgctaaccta gttgcggacc gtacgccagc ggtaggtaat acttttacta
     3661 cttcttttaa tacaggtcat ggtaaaaaat ggttcgtaga tggtaaggtt tctaaggatt
     3721 ctgagtggaa ttatcgttca gtatcaggtg ttcttccaac atggcgctgg tggcagactt
     3781 caacagggga aaaacttcgt gcagaatatg attttacaga tgcctataat ggcggaaatt
     3841 cccttaaatt ctctggtgat gtagccggta agacagatca ggatgtgaga ctttattcta
     3901 ctaagttaga agtaactgag aagaccaaac ttcgtgttgc ccacaaggga ggaaaaggtt
     3961 ctaaagttta tatggcattc tctacaactc cagactacaa attcgatgat gcagatgcat
     4021 ggaaagagct aaccctttct gacaactgga caaatgaaga atttgatctt agctcactag
     4081 cgggtaaaac catctatgca gtcaaactat ttttcgagca tgaaggtgct gtaaaagatt
     4141 atcagtttaa cctaggacaa ttaactatct cggacaatca ccaagagcca caatcgccga
     4201 caagcttttc tgtagtgaaa caatctctta aaaatgccca agaagcggaa gcagttgtgc
     4261 aatttaaagg caacaaggat gcagatttct atgaagttta tgaaaaagat ggagacagct
     4321 ggaaattact aactggctca tcttctacaa ctatttatct accaaaagtt agccgctcag
     4381 caagtgctca gggtacaact caagaactga aggttgtagc agtcggtaaa aatggagttc
     4441 gttcagaagc tgcaaccaca acctttgatt ggggtatgac tgtaaaagat accagcctac
     4501 caaaaccact agctgaaaat atcgttccag gtgcaacagt tattgatagt actttcccta
     4561 agactgaagg tggagaaggt attgaaggta tgttgaacgg taccattact agcttgtcag
     4621 ataaatggtc ttcagctcag ttgagtggta gtgtggatat tcgtttgacc aagcaacgta
     4681 ccgttgttag atgggtcatg gatcatgcag gggctggtgg tgagtctgtt aacgatggct
     4741 tgatgaacac caaagacttt gacctttatt ataaagatgc agatggtaag tggaagctag
     4801 ctaaggaagt ccgtggtaac aaagcacacg tgacagatat cactcttgat aaaccaatca
     4861 ctgctcaaga ctggcgcttg aatgttgtca cttctgacaa tggaactcca tggaaggcta
     4921 ttcgtatcta taactggaaa atgtatgaaa agcttgatac tgagagtgtc aatattccga
     4981 tggccaaggc tgcagcccgt tctctaggca ataacaaggt acaagttggc tttgcagatg
     5041 taccggctgg agcaactatt accgtttatg ataatccaaa ttctcaaatt ccgttcgcaa
     5101 ccttgaagag cgaagttgga ggagacctag caagtgcacc attggatttg acaaatcaat
     5161 ctggtcttct ttattatcgt acccagttgc caggcaagga aattagtaat gtcctagcag
     5221 tttccgttcc aaaagatgac agaagaatca agtcagtcag cctagaaaca ggacctaaga
     5281 aaacaagcta cgccgaaggg gaggatttgg accttagagg tggtgttctt cgagttcagt
     5341 atgaaggagg aactgaggac gaactcattc gcctaactca cgcaggtgta tcagtatcag
     5401 gttttgatac gcatcataag ggagaacaga atcttactct ccaatatttg ggacaaccag
     5461 taaatgctaa tttgtcagtg actgtcactg gccaagacga agcaagtccg aaaactattt
     5521 tgggaattga agtaagtcag gaaccgaaaa aagattacct agttggtgat agcttagact
     5581 tgtctgaagg acgctttgca gtggcttata gcaatgacac catggaagaa cattccttta
     5641 ctgatgaggg agttgaaatt tctggttacg atgctcaaaa gactggtcgt caaaccttga
     5701 cgcttcatta ccaaggccat gaagttagct ttgatgtttt ggtatctcca aaagcagcat
     5761 tgaacgatga gtacctcaaa caaaaattag cagaagttga agctgctaag aacaaggtgg
     5821 tctataactt tgcttcacca gaagtaaaag aagctttctt gaaagcaatt gaagcggccg
     5881 aacaagtgtt gaaagaccat gaaattagca cccaagatca agtcaatgac cgacttaata
     5941 aattgacaga agctcataaa gctctgaatg gtcaagagaa atttaaggaa gaaaagacag
     6001 agcttgatcg tttaacaggt gaggttcaag aactcttggc tgccaaacca aaccatcctt
     6061 caggttctgc cctagctccg cttcttgaga aaaacaaggc cttggttgaa aaagtagatt
     6121 tgagtccaga agagcttgca acagcgaaac agagtctaaa agatctggtt gctttattga
     6181 aagaagacaa gccagcagtc ttttctgata gtaaaacagg tgttgaagta cacttctcaa
     6241 ataaagagaa gactgtcatc aagggtttga aagtagagcg tgttcaagca agtgctgaag
     6301 agaagaaata ctttgctgga gaagatgctc atgtctttga aatagaaggt ttggatgaaa
     6361 aaggtcaaga tgttgatctc tcttatgctt ctattgtgaa aatcccaatt gaaaaagata
     6421 agaaagttaa gaaagtattt ttcttacctg aaggcaaaga ggcagtagaa ttggcttttg
     6481 aacaaacgga tagtcatgtt atctttacag cacctcactt tactcattat gcctttgttt
     6541 atgaatctgc tgaaaaacca caacctgcta aaccagcacc acaaaacaca gtccttccaa
     6601 aacctactta tcaaccggct tctgatcaac aaaaggctcc taaattggaa gttcaagagg
     6661 aaaaggttgc ctttcatcgt caagagcatg aaaatgctga gatgctagtt ggggaacaac
     6721 gagtcatcat acagggacga gatggactgt taagacatgt ctttgaagtt gatgaaaagg
     6781 gtcagcgtcg tcttcgttca acagaagtca tccaagaagc gattccagaa attgttgaaa
     6841 ttggaacaaa agtaaaaaca gtaccagcag tagtagctac acaggaaaaa ccagctcaaa
     6901 atacagcagt taaatcagaa gaagcaagca aacaattgcc aaatacagga acagctgatg
     6961 ctaatgaaac cctaatagca ggcttagcca gcctcggtct tgctagttta gccttgacct
     7021 tgagacggaa aagagaagat aaagattaaa tatcgaaaaa tcttgtgaaa tctttcctta
     7081 tatttccaaa gtgtgatata atagtttcga ataaaataaa taaaggagtt tttgtaacat
     7141 ggcaaaactt actgttaaag acgttgactt gaaaggtaaa aaagtcctcg ttcgtgttga
     7201 cttcaacgta ccattgaaag atggcgtaat cactaacgat aaccgtatca cagcagctct
     7261 tccaactatt aagtgcatca tcgaacaagg tggacgtgca attcttttct ctcaccttgg
     7321 acgtgtgaaa gaagaagctg ataaagctgg taaatcactt gctcctgtag cagctgactt
     7381 ggcagcaaaa cttggtcaag atgttgtttt cccaggtgtc actcgtggtg ctgaattaga
     7441 agcggcaatc aacgctcttg aagatggaca agttctcttg gttgaaaaca ctcgttacga
     7501 agatgttgac ggcaagaaag aatctaaaaa cgatcctgaa cttggtaaat actgggcatc
     7561 acttggagat ggtatcttcg taaacgatgc attcggtaca gctcaccgtg cacacgcatc
     7621 taacgttggt atctcagcaa acgttgaaaa agcagttgct ggtttccttc ttgaaaacga
     7681 aattgcctac atccaagaag cagttgaaac tccagaacgt ccattcgtgg ctatccttgg
     7741 tggttcaaaa gtttcagaca agatcggtgt tatcgaaaac ttgcttgaaa aagctgataa
     7801 agtccttatc ggtggtggga tgacttacac attctacaaa gcacaaggta tcgaaatcgg
     7861 taactcactt gtagaagaag acaaattgga tgttgcgaaa gctcttcttg aaaaagcaaa
     7921 tggtaaattg atcttgccag ttgactcaaa agaagctaac gcatttgctg gttacactga
     7981 agtgcgtgac actgaaggtg aagcagtttc tgaaggcttc cttggtcttg acatcggtcc
     8041 aaaatctatc gccaaatttg acgaagcttt gactggtgcc aaaacagtcg tatggaacgg
     8101 acctatgggt gtatttgaaa acccagattt ccaagctggt acaatcggtg tgatggacgc
     8161 tatcgtgaaa caaccaggag ttaaatcaat catcggtggt ggtgactcag ctgccgcagc
     8221 gattaacctt ggccgtgcag acaagttctc atggattagt acgggtggtg gagcatcaat
     8281 ggaacttctt gaaggtaagg ttcttccagg acttgcagcc ttgacagaaa aataagattt
     8341 tataaataaa tcaaagaaga gagggatgaa agttcctctt ttcttttgct taaaataaaa
     8401 acgcttcctc tcaactatta ctcataaaaa tcaccgattt atgataaaat ggaaatagaa
     8461 agttgagatt atgagttatt ttaaaaaata taaattcgat aaatcccagt tcaaacttgg
     8521 tatgcgaacc tttaaaacag gtattgctgt ttttctagtt ctcttgattt ttggcttttt
     8581 tggctggaaa ggtcttcaaa ttggtgcttt gacagccgtt tttagcctga gggagagttt
     8641 tgatgagagt gttcattttg ggacttcgcg tattctagga aatagtatcg gtggactcta
     8701 tgccttggtc ttcttcttat taaatacctt tttccacgaa gccttttggg tgaccttggt
     8761 agttgttcca atctgcacca tgttaaccat tatgacaaat gtagccatga ataacaaagc
     8821 aggggttatt ggtggtgtag cagctatgtt aatcattacc ctatcaattc caagtggtga
     8881 gacaattttg tacgtgtttg tgcgtgtatt agaaacgttt atgggagttt ttgtcgcaat
     8941 tatcgtaaat tacgatattg atcgtattcg tctcttttta gagaaaaaag aaaaataatg
     9001 ttacatttta taacattatc aattgacgtt tgtctttttt tagactataa tagacagaaa
     9061 gaaggaaatt gtaaatgaag gaaaaagaat ttcgccgaaa tatggctgtt tttcctatcg
     9121 gcagtgttat gaagttgacc gatctatcgg cgcgtcagat tcgttattat gaagatcaag
     9181 agttgatcaa gcccgatcga aacgaaggaa atcgtcgcat gtattccttg aatgacatgg
     9241 atcgtctgct tgaaatcaaa gattatatct ctgaaggtta taatatcgct gccattaaga
     9301 aaaaatatgc tgaacgtgaa gcgaaatcca agaaagcggt tagtcagact gaagtacgtc
     9361 gtgcacttca caatgaactc ctccaacagg ggcgttttgc ttcagtacag tcaccttttg
     9421 gtcgcggtta ggcaaccgca agtagtcata gattaaggag aaaacttatg ccaatcacag
     9481 ctgcagatat tcgtcgtgaa gtcaaggaaa aaaatgttac ctttattcgt cttatgttct
     9541 cagatatttt gggaaccatg aaaaacgtcg aaattcctgc tacagatgaa cagttagata
     9601 aggtcttgtc gaacaaggtt atgtttgatg gatcttctat tgaaggtttt gtacgtatca
     9661 atgagtcgga tatgtacttg tacccggact tggatacatg gacagtcttc ccttggggag
     9721 atgaaaatgg aagtgttgca ggtctgatct gtgatgtcta tacaacagaa ggtgaaccat
     9781 ttgcgggtga ccctcgtggt aatttgaaac gagctcttcg tcacatggaa gaagttggat
     9841 tcaaatcctt caaccttggt ccagagccag aattcttcct atttaagttg gatgaaaatg
     9901 gggacccaac acttgaagtg aatgacaagg gtggctactt tgacttggca cctactgacc
     9961 ttgcggacaa cacacgtcgt gagattgtga atgtcttgac caaaatggga tttgaagtag
    10021 aagcgagtca ccacgaggtt gcggttggac agcatgagat tgactttaag tacgatgaag
    10081 ttctccgtgc ttgtgataag attcaaatct ttaagcttgt tgttaaaacc attgctcgca
    10141 aacacggact ttacgcaaca tttatggcga agccaaaatt tggtattgct ggatcaggta
    10201 tgcactgtaa tatgtccttg tttgatgcag aaggaaataa cgccttcttt gatccaaatg
    10261 atccaaaagg aatgcagttg tcagaaacag cttaccattt cctaggcggt ttgatcaagc
    10321 atgcttacaa ctatactgcc atcatgaacc caacagttaa ctcatacaaa cgtttggttc
    10381 caggttatga agcgcctgtt tacattgctt gggctggtcg taaccgttcg ccacttgtgc
    10441 gcgtacctgc ttcacgtggt atgggaactc gtcttgagtt gcgttcagtg gatccaatgg
    10501 cgaaccctta cgttgctatg gctgttcttt tggaagttgg tttgtatggt attgaaaata
    10561 aaatcgaagc accagctcct atcgaagaaa atatctacat catgacagca gaagagcgca
    10621 aggaagctgg tattacagac cttccatcaa ctcttcacaa cgctttgaaa gctttgacag
    10681 aagatgaagt ggttaaagct gctctcggag atcacatcta tactagcttc cttgaagcca
    10741 aacgaatcga atgggcaagt tatgcaacct tcgtttcaca atgggaaatt gataattatt
    10801 tagaccttta ctaatactaa tatagaagaa agattgcctg tgggtaaaca gtcacacagc
    10861 agtctaagcc atcaaatacc tataaccctt aaaatagcga tttcggtcgc tatttatttt
    10921 tcgaaaaatc ctcttgactc ttttgcgaat tagatttatc atcagacgtg tagaagaaaa
    10981 tcaaggagtc actatggaaa aattcaattt caaaaacaat atcgggcaag aaaacaagtt
    11041 acttcaaatc gaaatctaca agtttactaa cttttgcaag cttcaaaatt acacatctgt
    11101 aaatattttt tcgaaagata tatttgaagc catagtaaat tgagactaga atagtacact
    11161 acatctgcta aaaatttcta gaaattaatt taactttcct aatcgatttg tttacatctt
    11221 atttcaatct actatattat ctcaataaat gatgatggcg actgttttgt ttgtgcttgt
    11281 cgatttttga atcctgatcg agcggaagaa tatttctgcc gaggttgtca aggggaaaat
    11341 cctgaagata ttgaatttta tgatgaacaa ttacaggcag aaaaggtgga ggatttgaat
    11401 attcgattgg aagtaaaaaa ttgaattatc tacaatctca tcaacaataa acctatttca
    11461 accccattat ctataggcgt atttttacgc ctttttatat acttgaagca cataagaaac
    11521 attatgggaa gtttgctgat ggaagcattc aagaaattga tgttccttat gatattcctg
    11581 atacttggga gtgggtgagg ttttctacat tggttgaaat tgtcagaggt ggctctccac
    11641 gaccaatcaa agattatctt acttctgaag tagatggaat aaattggata aaaataggtg
    11701 atactgaaaa gggtgaaaag tatataaata atgttaaaga aaaaatcaaa aaatcagggc
    11761 ttaacaaaac tagatttgta aaaaaaggta catttttgtt aactaattct atgagttttg
    11821 gtagacctta tattttgaat gttgatggtg caatacacga tggatggttg gctatttcga
    11881 actatgaaaa ctcattaaat aaagattacc tattctatat tctttcatca aatgtagttt
    11941 attctcaatt tctatctcta attagtggag ctgttgtgaa aaacttgaat agtgataaag
    12001 ttgcttctat tcttatccct ctccccccac tatccgaaca acaacgaata gtagaagcaa
    12061 tcgaatcagc tttagaaaaa gtagatgaat atgctgaaag ttataataga ctagaacagc
    12121 tagataaaga atttccagat aaactaaaaa aatctattct tcaatatgct atgcaaggaa
    12181 aattagttga acaagaccca aatgatgaat cagtcgaagt tttacttgaa aaaatacgag
    12241 cagaaaaaca aaaactcttt gaagaaggca agattaaaaa gaaagatttg gacatttcta
    12301 ttgtttccca aggagatgat aactcttatt atgaggaagt accttgtgaa ataccagaaa
    12361 gttgggaatg ggtgaggtta aacgatataa cctcctacat tcaaagaggg aagtctccaa
    12421 aatattcaaa tattcctata taccctgtga ttgctcagaa atgtaatcaa tggtctggct
    12481 tttctattga cttagcaaga tttattgatc ccgaaacagt tcatagttac cagaaagaaa
    12541 gattactaag ggatggtgat ttaatgtgga attcaacagg tctaggtacg ttaggacgat
    12601 tagctattta ccatgaaaat aaaaatcctt atgcctgggc tgttgcagat agccatgtta
    12661 cagttataag agtcttatcg ggggttatta attgccattt catctataat tttctttcct
    12721 ctcctattgt tcagtcagtt attgaagaaa aagcgtcggg tagtacaaaa caaaaagaat
    12781 tattaacgaa aactataaaa gaatatttaa tccccctccc accccttcct gaacaatcca
    12841 gaatcgttga taaaatcgaa caatttttcg cccatattga cgcactaatt tagtataata
    12901 gttttacgtg cggtggaatt tctatcaaac ataagatagg agttttacgt tgaaagattt
    12961 tgaattacat ttgaagaaag ctggtttagc tgaaaatact gtgcgttcgt acctttatgg
    13021 tgttcgattt ttcttggaga attacgaact caaaatggaa gatttatttg aatacaaagg
    13081 atatttatta gacaatttta aacctaaaac tgtcaatttg cgactacaag gagtaaataa
    13141 atatctggct tttattggtc atgatgattt gaaattaaaa tttgtcaaag tacagcaaaa
    13201 accgttttta gaagatgtca ttagccatgc tgattacctt tttcttaaac gtagtttgaa
    13261 aaaagatggt attttaaaat ggcattttgt cgtttggttt ttaggtgcaa ctggggctcg
    13321 tgtaagtgaa cttattaaac taaaagtaga gcatgtagaa atcggttatt ttgacattta
    13381 ctccaaaggt ggtaaaattc gtagactgta cattcctaaa aaattaagaa atagttgtct
    13441 tagttggtta gaatcagaaa atcgtcgaag tggttattta tttttgaata aatttaacga
    13501 accaattaca gcaagaggag ttgctcaaca gttaaaaaat tatgctgata aatacaaaat
    13561 gaatcctaaa gtaatttacc ctcattcttt taggcattta tttgctaaga attttttagc
    13621 gaagtataat gatattgcct tgcttgcaga tttgatggga cacgaaagta tagaaactac
    13681 tcgaatttat ctaaggaaaa cagctactga acaacaaaat attgtagata aaattgttaa
    13741 ttggtaaaaa ataacaggtg gtcaaactga ctacctgcta tttttgtgat tatg
//
LOCUS       DAVLVP010000048        13201 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.048, whole genome shotgun sequence.
ACCESSION   DAVLVP010000048 DAVLVP010000000
VERSION     DAVLVP010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 13201)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13201)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13201
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.048"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            70..5640
                     /gene="zmpC"
                     /locus_tag="VYN69_001316"
     CDS             70..5640
                     /gene="zmpC"
                     /locus_tag="VYN69_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloprotease ZmpC"
                     /protein_id="HEV1533581.1"
                     /translation="MSRKSIGEKRHSFSMRKLSVGLVSVTVSSFFLMSQGIQSVSADN
                     MESPIHYKYMTEGKLTDEEKSLLVEALPQLAEESDDTYYLVYRSQQFLPNTGFNPTVG
                     TFLFTAGLSLLVLLVSKRENGKKRLVHFLLLTSMGVQLLPASAFGLTSQILSAYNSQL
                     SIGVGEHLPEPLKIEGYQYIGYIKTKKQDNTELSRTVDGKYSAQRDSQPNSTKTSDVV
                     HSADLEWNQGQGKVSLQGEASGDDGLSEKSSIAADNLSSNDSFASQVEQNPDHKGESV
                     VRPTVPEQGNPVSATTVQSAEEEVLATTNDRPEYKLPLETKGTQEPGHEGEAAVREDL
                     PVYTKPLETKGTQGPGHEGEAAVREEEPAYTEPLATKGTQEPGHEGKATVREETLEYT
                     EPVATKGTQEPEHEGEAAVEEELPALEVTTRNRTEIQNIPYTTEEIQDPTLLKNRRKI
                     ERQGQAGTRTIQYEDYIVNGNVVETKEVSRTEVAPVNEVVKVGTLVKVKPTVEITNLT
                     KVENKKSITVSYNLIDTTSAYVSAKTQVFHGDKLVKEVDIENPAKEQVISGLDYYTPY
                     TVKTHLTYNLGENNEENTETSTQDFQLEYKKIEIKDIDSVELYGKENDRYRRYLSLSE
                     APTDTAKYFVKVKSDRFKEMYLPVKSITENTDGTYKVTVAVDQLVEEGTDGYKDDYTF
                     TVAKSKAEQPGVYTSFKQLVTAMQSNLSGVYTLASDMTADEVSLGDKQTSYLTGAFTG
                     SLIGSDGTKSYAIYDLKKPLFDTLNGATVRDLDIKTVSADSKENVAALAKAANSANIN
                     NVAVEGKISGAKSVAGLVASATNTVIENSSFTGKLIANHQDSNKNDTGGIVGNITGNS
                     SRVNKVRVDALISTNARNNNQTAGGIVGRLENGALISNSVATGEIRNGQGYSRVGGIV
                     GSTWQNGRVNNVVSNVDVGDGYVITGDQYAAADVKNASTSVDNRKADRFATKLSKDQI
                     DAKVADYGITVTLDDTGQDLKRNLREVDYTRLNKAEAERKVAYSNIEKLMPFYNKDLV
                     VHYGNKVATTDKLYTTELLDVVPMKDDEVVTDINNKKNSINKVMLHFKDNTVEYLDVT
                     FKENFINSQVIEYNVTGKEYIFTPEAFVSDYTAITNNVLSDLQNVTLNSEATKKVLGA
                     ANDAALDNLYLDRQFEEVKANIAEHLRKVLAMDKSINTTGDGVVEYVSEKIKNNKEAF
                     MLGLTYMNRWYDINYGKMNTKDLSTYKFDFNGNNETSTLDTIVALGNSGLDNLRASNT
                     VGLYANKLASVKGEDSVFDFVEAYRKLFLPNKTNNEWFKENTKAYIVEMKSDIAEVRE
                     KQESPTADRKYSLGVYDRISAPSWGHKSMLLPLLTLPEESVYISSNMSTLAFGSYERY
                     RDSVDGVILSGDALRTYVRNRVDIAAKRHRDHYDIWYNLLDSASKEKLFRSVIVYDGF
                     NVKDETGRTYWARLTDKNIGSIKEFFGPVGKWYEYNSSAGAYANGSLTHFVLDRLLDA
                     YGTSVYTHEMVHNSDSAIYFEGNGRREGLGAELYALGLLQSVDSVNSHILALNTLYKA
                     EKDDLNRLHTYNPVERFDSDEALQSYMHGSYDVMYTLDAMEAKAILAQNNDVKKKWFR
                     KIENYYVRDTRHNKDTHAGNKVRPLTDEEVANLTSLNSLIDNDIINRRSYDDNREYKR
                     NGYYTISMFSPVYAALSNSKGAPGDIMFRKIAYELLAEKGYHKGFLPYVSNQYGAEAF
                     ASGSKTFSSWHGRDVALVTDDLVFKKVFNGEYSSWADFKKAMFKQRIDKQDNLKPITI
                     QYELGNPNSTKEVTITTAAQMQQLINEAAAKDITNIDRATSHTPASWVHLLKQKIYNA
                     YLRTTDDFRNSIYK"
     gene            5996..6850
                     /locus_tag="VYN69_001317"
     CDS             5996..6850
                     /locus_tag="VYN69_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000220702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEV1533582.1"
                     /translation="MTYEYQSHIYLAEAVLNVKDLVSQTVFYQQIIGLEILSQTDTEV
                     VLGLGGKALVHLIQAQEGGEVREHYGLYHLAILLPTRKALADVLKHLTDLQIPLVGGA
                     DHGYSEALYLEDLEGNGIELYRDKPVSTWDIREDGRIIGVTEVLAAQDIYELGERVEP
                     FILAEGTRMGHIHLSVKDSRKSRQFYQTVLGLEDKFSVPSASWIAAGDYHHHLAVNEW
                     GGKGLDPRKQVLPGLAYYVIEVAHKEELLTIAQRAQEVDAPIKWMTSIQLEITDSDGI
                     VTRIRLAR"
     gene            6956..7513
                     /locus_tag="VYN69_001318"
     CDS             6956..7513
                     /locus_tag="VYN69_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000200249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEV1533583.1"
                     /translation="MTSEYQKMIAGEFYRPSDPELRALAQASRQKQAAFNKEENPLKG
                     AEIIKTWFASTGKNLYINTRLMVDYGVNIHLGENFYSNWNLTMLDICPIRIGDNAMIG
                     PNCQFLTPLHPLDPQERNSGIEYGKPITIGDNFWTGGGVIVLPGVTLGNNVVAGAGAV
                     ITKSFGDNVVLAGNPARVIKEIPVK"
     gene            7730..8494
                     /locus_tag="VYN69_001319"
     CDS             7730..8494
                     /locus_tag="VYN69_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HEV1533584.1"
                     /translation="MIQKHAIPILEFDDNPQAVIMPNHEGLDLQLPKKCVYAFLGEEI
                     DRYAREVGANCVGEFVSATKTYPVYVVNYKDEEICLVQAPVGSAPAAQFMDWLIGYGV
                     EQIISTGTCGVLADIEENAFLVPVRALRDEGASYHYVAPSRYMEMQPEAIAAIERVLD
                     DRGIPYEEVMTWSTDGFYRETAEKVAYRKEEDCAVVEMECSALAAVAQLRGVLWGELL
                     FTADSLADLDQYDSRDWGSEAFNKALELSLASVHHL"
     gene            9107..10486
                     /locus_tag="VYN69_001320"
     CDS             9107..10486
                     /locus_tag="VYN69_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000897732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEV1533585.1"
                     /translation="MLFKLSKEKIELGLSRLSPARRIFLSFALVILLGSLLLSLPFVQ
                     VESSRATYFDHLFTAVSAVCVTGLSTLPVAHTYNIWGQIICLLLIQIGGLGLMTFIGV
                     FYIQSKQKLSLRSRATIQDSFSYGETRSLRKFVYSIFLTTFLVESLGAILLSFRLIPQ
                     LGWGRGLFSSIFLAISAFCNAGFDNLGSTSLFAFQTDLLVNLVIAGLIITGGLGFMVW
                     FDLAGHVGRKKKGRLHFHTKLVLLLTIGLLLFGTATTLFLEWNNAGTIGNLPVADKVL
                     VSFFQTVTMRTAGFSTIDYTQAHPVTLLIYILQMFLGGAPGGTAGGLKITTFFVLLVF
                     ARSELLGLPHANVARRTIAPRTVQKSFSVFIIFLMSFLIGLILLGITAKGNPPFIHLI
                     FETISALSTVGVTANLTPDLGKLALSVIMPLMFMGRIGPLTLFVSLADYHPEKKDMIH
                     YMKADISIG"
     gene            10500..11165
                     /locus_tag="VYN69_001321"
     CDS             10500..11165
                     /locus_tag="VYN69_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001283913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEV1533586.1"
                     /translation="MSDRTIGILGLGIFGSSVLAALAKQDMNIIAIDDHAERINQFEP
                     VLARGVIGDITDEELLRSAGIDTCDTVVVATGENLESSVLAVMHCKSLGVPTVIAKVK
                     SQTAKKVLEKIGADSVISPEYEMGQSLAQTILFHNSVDVFQLDKNVSIVEMKIPQSWA
                     GQSLSKLDLRGKYNLNILGFREQENSPLDVEFGPDDLLKADTYILAVINNQYLDTLVA
                     LNS"
     gene            11300..11458
                     /locus_tag="VYN69_001322"
     CDS             11300..11458
                     /locus_tag="VYN69_001322"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533587.1"
                     /translation="MKIKDQTRKLATGCLKHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     NIYTARRC"
     gene            11520..11693
                     /locus_tag="VYN69_001323"
     CDS             11520..11693
                     /locus_tag="VYN69_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1533588.1"
                     /translation="MKLLSIAISSYNAAAYLHYCVESLVIGWDQVGNFSKGQYIMMHS
                     LIYRTDLLRASQF"
     gene            11760..12170
                     /locus_tag="VYN69_001324"
     CDS             11760..12170
                     /locus_tag="VYN69_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004262849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1533589.1"
                     /translation="MYYLPVDFYRYLIGREGQSVNEQVMIKCIDQQLKVNRLLVDQLD
                     LSQVSHPKMREYLLNHIEITTVISSTLLNRSETAEHLAKKRQLWTYIQQKNPEVFQAI
                     RKTMLSRLTKHSVLPDRKLSNVVYQITKSVYGFN"
     gene            12286..>13201
                     /gene="pavB"
                     /locus_tag="VYN69_001325"
     CDS             12286..>13201
                     /gene="pavB"
                     /locus_tag="VYN69_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding SSURE repeat adhesin PavB"
                     /protein_id="HEV1533590.1"
                     /translation="MKFNPNQRYTRWSIRRLSVGVASVVVASGFFVLVGQPSSVRADV
                     VNPTPGQVLPEETSGTKEGDLSEKPGDTVLTQAKPEGVTGNTNSLPTPTERTEVSEET
                     NSSSLDTLFEKDEEAQKNPELTDVLKETVDTADVDGTQASPAETTPEQVKGGVKENTK
                     DSIDVPAAYLEKAEGKGPFTAGVNQVIPYELFAGDGMLTRLLLKASDNAPWSDNGTAK
                     NPALPPLEGLTKGKYFYEVDLNGNTVGKQGQALIDQLRANGTQTYKATVKVYGNKDGK
                     ADLTNLVATKNVDININGLVAKETVEKAV"
BASE COUNT         4224 a         2296 c         2938 g         3743 t
ORIGIN      
        1 ttttatgttt atacttttat gctataaaat atagattgat ataaagaata tagaaaaagc
       61 gaggttaata tgagccgaaa aagcattggt gagaaacgcc atagtttctc gatgagaaag
      121 ttgtcagtgg gattggtatc agttactgta tctagtttct ttttgatgag tcaagggatt
      181 caatcggtat cggccgataa tatggaaagt ccaattcatt ataagtatat gaccgagggt
      241 aaattgacag acgaggaaaa atccttgctg gtagaggccc ttccacaact ggctgaagaa
      301 tcagatgata cttattactt ggtttataga tctcaacagt ttttaccgaa tacaggtttt
      361 aacccaactg ttggtacttt cctttttact gcaggattga gcttgttagt tttattggtt
      421 tctaaaaggg aaaatggaaa gaaacgactt gttcattttc tgctgttgac tagcatggga
      481 gttcaattgt tgccggccag tgcttttggg ttgaccagcc agattttatc tgcctataat
      541 agtcagcttt ctatcggagt cggggaacat ttaccagagc ctctgaaaat cgaaggttat
      601 caatatattg gttatatcaa aactaagaaa caggataata cagagctttc aaggacagtt
      661 gatgggaaat actctgctca aagagatagt caaccaaact ctacaaaaac atcagatgta
      721 gttcattcag ctgatttaga atggaaccaa ggacagggga aggttagttt acaaggtgaa
      781 gcatcagggg atgatggact ttcagaaaaa tcttctatag cagcagacaa tctatcttct
      841 aatgattcat tcgcaagtca agttgagcag aatccggatc acaaaggaga atctgtagtt
      901 cgaccaacag tgccagaaca aggaaatcct gtgtctgcta caacggtgca gagtgcggaa
      961 gaggaagtat tggcgacgac aaatgatcga ccagagtata aacttccatt ggaaaccaaa
     1021 ggcacgcaag aacccggtca tgagggtgaa gccgcagtcc gtgaagactt accagtctac
     1081 actaagccac tagaaaccaa aggtacacaa ggacccggac atgaaggtga agctgcagtt
     1141 cgcgaggaag aaccagctta cacagaaccg ttagcaacga aaggcacgca agagccaggt
     1201 catgagggca aagctacagt ccgcgaagag actctagagt acacggaacc ggtagcgaca
     1261 aaaggcacac aagaacccga acatgagggc gaagcggcag tagaagaaga acttccggct
     1321 ttagaggtca ctacacgaaa tagaacggaa atccagaata ttccttatac aacagaagaa
     1381 attcaggatc caacacttct gaaaaatcgt cgtaagattg aacgacaagg gcaagcaggg
     1441 acacgtacaa ttcaatatga agactacatc gtaaatggta atgtcgtaga aactaaagaa
     1501 gtgtcacgaa ctgaagtagc tccggtcaac gaagtcgtta aagtaggaac acttgtgaaa
     1561 gttaaaccta cagtagaaat tacaaactta acaaaagttg agaacaaaaa atctataact
     1621 gtaagttata acttaataga cactacctca gcatatgttt ctgcaaaaac gcaagttttc
     1681 catggagaca agctagttaa agaggtggat atagaaaatc ctgccaaaga gcaagtaata
     1741 tcaggtttag attactacac accgtataca gttaaaacac acctaactta taatttgggt
     1801 gaaaataatg aggaaaatac tgaaacatca actcaagatt tccaattaga gtataagaaa
     1861 atagagatta aagatattga ttcagtagaa ttatacggta aagaaaatga tcgttatcgt
     1921 agatatttaa gtctaagtga agcgccgact gatacggcta aatactttgt aaaagtgaaa
     1981 tcagatcgct tcaaagaaat gtacctacct gtaaaatcta ttacagaaaa tacggatgga
     2041 acgtataaag tgacggtagc cgttgatcaa cttgtcgaag aaggtacaga cggttacaaa
     2101 gatgattaca catttactgt agctaaatct aaagcagagc aaccaggagt ttacacatcc
     2161 tttaaacagc tggtaacagc catgcaaagc aatctgtctg gtgtctatac attggcttca
     2221 gatatgaccg cagatgaggt gagcttaggc gataagcaga caagttatct cacaggtgca
     2281 tttacaggga gcttgatcgg ttctgatgga acaaaatcgt atgccattta tgatttgaaa
     2341 aaaccattat ttgatacatt aaatggtgct acagttagag atttggatat taaaactgtt
     2401 tctgctgata gtaaagaaaa tgtcgcagcg ctggcgaagg cagcgaatag cgcgaatatt
     2461 aataatgttg cagtagaagg aaaaatctca ggtgcgaaat ctgttgcggg attagtagcg
     2521 agcgcaacaa atacagtgat agaaaacagc tcgtttacag ggaaacttat cgcaaatcac
     2581 caggacagta ataaaaatga tactggagga atagtaggta atataacagg aaatagttcg
     2641 agagttaata aagttagggt agatgcctta atctctacta atgcacgcaa taataaccaa
     2701 acagctggag ggatagtagg tagattagaa aatggtgcat tgatatctaa ttcggttgct
     2761 actggagaaa tacgaaatgg tcaaggatat tctagagtcg gaggaatagt aggatctacg
     2821 tggcaaaacg gtcgagtaaa taatgttgtg agtaacgtag atgttggaga tggttatgtt
     2881 atcaccggtg atcaatacgc agcagcagat gtgaaaaatg caagtacatc agttgataat
     2941 agaaaagcag acagattcgc tacaaaatta tcaaaagacc aaatagacgc gaaagttgct
     3001 gattatggaa tcacagtaac tcttgatgat actgggcaag atttaaaacg taatctaaga
     3061 gaagttgatt atacaagact aaataaagca gaagctgaaa gaaaagtagc ttatagcaac
     3121 atagaaaaac tgatgccatt ctacaataaa gacctagtag ttcactatgg taacaaagta
     3181 gcgacaacag ataaacttta cactacagaa ttgttagatg ttgtgccgat gaaagatgat
     3241 gaagtagtaa cggatattaa taataagaaa aattcaataa ataaagttat gttacatttc
     3301 aaagataata cagtagaata cctagatgta acattcaaag aaaacttcat aaacagtcaa
     3361 gtaatcgaat acaatgttac aggaaaagaa tatatattca caccagaagc atttgtttca
     3421 gactatacag cgataacgaa taacgtacta agcgacttgc aaaatgtaac acttaactca
     3481 gaagctacta aaaaagtact aggagcagcg aatgatgcag ccttagataa cctatactta
     3541 gatagacaat ttgaagaagt taaagctaat atagcagaac acctaagaaa agtattagcg
     3601 atggataaat caatcaatac tacaggagac ggtgtagttg aatacgtaag tgagaaaatc
     3661 aaaaataaca aagaagcatt tatgctaggt cttacttata tgaaccgttg gtacgatatt
     3721 aattatggta aaatgaatac aaaagattta tctacgtaca agtttgactt taacggaaat
     3781 aatgagactt caacgttgga tactattgtc gcattaggaa atagtggact agataacctg
     3841 agagcttcaa atactgtagg tttatatgcg aataaacttg catcggtaaa aggagaagat
     3901 tcagtctttg acttcgtaga agcgtataga aaactgttct taccaaacaa aacaaataac
     3961 gagtggttta aagaaaatac aaaggcatat atagtcgaaa tgaagtctga tattgcagaa
     4021 gtacgagaaa aacaagaatc accaacagcc gatagaaaat attcattagg agtttacgat
     4081 agaatatcag caccaagttg ggggcataag agtatgttat taccactact aactttacct
     4141 gaagaatctg tgtatatttc atcgaatatg tctacacttg cattcggttc gtatgaaaga
     4201 tatcgtgata gtgtggatgg agttattctt tcaggagatg ctttacgaac ttatgtaaga
     4261 aatagagttg atatagcagc gaaaaggcat agagaccatt atgatatttg gtacaatctt
     4321 cttgacagtg cttcaaaaga aaaacttttc cgttctgtga tagtttatga tggattcaat
     4381 gtaaaagatg agacaggaag aacttattgg gcaaggttaa cggataaaaa catcggctct
     4441 attaaagaat tcttcggacc tgttgggaaa tggtatgagt ataatagtag tgcaggagcg
     4501 tatgcgaatg gaagtttaac gcactttgtg ttagatagat tattagatgc ttatggaacg
     4561 tcggtttata ctcatgaaat ggttcataat tctgattctg caatctactt tgaaggaaat
     4621 ggtagacgtg aaggattggg agcggagtta tacgcacttg gtttactgca atctgtagat
     4681 agtgtaaatt ctcatatttt agctttaaat acgttatata aagcagaaaa agatgatttg
     4741 aatagattgc atacatataa tccggtggaa cgtttcgatt cggatgaggc gcttcaaagt
     4801 tatatgcatg gatcatatga tgtaatgtat acacttgatg cgatggaagc aaaagcgata
     4861 ttagctcaaa ataatgatgt taagaaaaaa tggtttagaa aaatagaaaa ttattacgtt
     4921 cgtgatacta gacataataa agatacacat gcaggaaata aagtccgtcc attaacagat
     4981 gaagaagtag ctaacttaac atcgttaaac tcattaatcg acaacgacat cataaataga
     5041 cgtagctatg atgataatag agaatataaa cgaaatggct actatactat aagtatgttc
     5101 tctcctgtat acgcagcgct aagcaattcg aaaggtgctc ctggagatat tatgtttaga
     5161 aaaatagctt atgaattact tgcggaaaaa ggttatcaca aaggattcct accttatgtt
     5221 tctaatcagt acggagcaga agcatttgcc agcggaagca aaacattctc atcatggcat
     5281 ggaagagatg ttgctttagt gacagatgat ttagtattta agaaagtatt caatggtgag
     5341 tactcatcat gggctgattt caaaaaagca atgtttaaac aacgtataga taaacaagat
     5401 aatctgaaac caataacaat tcaatacgaa ttaggtaatc ctaatagtac aaaagaagta
     5461 actataacaa cggctgcaca aatgcaacaa ttaattaatg aagcggctgc gaaagatatt
     5521 actaatatag atcgtgcaac gagtcatacc ccagcaagtt gggtgcattt attaaaacaa
     5581 aaaatctata atgcatatct tcgcactaca gatgacttta gaaattctat atataaataa
     5641 gattgtagag tttcattgtt gagtagtgtt tcttgtaagg atgaggagtc agatgacaaa
     5701 tcgactcctt tttcttatgg atcgatgtag agatttgatt gaatgcagat tgcaggaatc
     5761 atcttcaact catcaacgac caatggtgac aaggtggatt tcaatcccac agaaaatgtt
     5821 gatttgagaa ataactttgc tagtctagta aaataaatac aaaacaatcc tagaagattt
     5881 tttctgggat tgttttttgc tgagtgggat gcttcaagtt gtctggcttg actttcttga
     5941 gggaagttat ataatagttg taataattaa agttacaata aaagaggaaa tatttatgac
     6001 atatgaatac cagagccaca tttatttagc agaggcagtt ttaaatgtga aggatttggt
     6061 cagtcaaaca gttttttatc agcagattat tggtttagaa atcctatctc aaacggatac
     6121 agaggtcgtt ctgggacttg gaggaaaagc cttggtacac ttgattcaag cacaagaggg
     6181 tggagaagta agggaacatt atggtcttta ccatctggct attcttttgc cgacacgaaa
     6241 ggctttggcg gatgtcttga agcacctgac ggatttacag attcctcttg ttggcggtgc
     6301 agatcacggt tacagtgagg ccctttactt agaggacttg gagggaaatg gcattgaact
     6361 ctatcgagat aagccagttt ccacatggga tattcgagaa gatggacgta ttatcggggt
     6421 gactgaagtc cttgcggctc aggatatcta tgagttgggg gaaagagtag agccttttat
     6481 cctagcagag ggtacgagaa tggggcatat tcatctttct gtcaaggata gtcgaaagtc
     6541 cagacagttt tatcaaacgg tgttagggct cgaggataaa ttcagtgtgc ctagtgctag
     6601 ttggatcgca gctggggact accatcatca tttagcagtc aacgaatggg gaggaaaagg
     6661 tctggatccg cgtaaacaag tcctaccagg tttagcctac tatgtcatcg aagtcgcaca
     6721 taaagaagaa ctgttaacga ttgcccaacg agcacaagaa gttgacgcac caatcaaatg
     6781 gatgacatcg atccaattgg aaatcacaga ctcagatggc atcgtgaccc gtattcgttt
     6841 agctagatag atggtatgtg atgaaggtag agcatcaatt gtagaaagtg ctccttattc
     6901 tagtcagata cgtggaaaat ttgatatgat agtagggaat aaggagaaga ataatatgac
     6961 tagcgaatac cagaaaatga tagcaggaga gttttaccgt ccatcagacc cagaactgag
     7021 agccttagct caggcttctc gccaaaaaca ggccgccttt aacaaggaag agaacccctt
     7081 gaagggagcc gaaatcatca agacttggtt tgcctcaacc gggaaaaatc tttacatcaa
     7141 cactcgcttg atggtggact acggtgtcaa catccatcta ggggaaaatt tttattctaa
     7201 ttggaacttg accatgctgg atatctgtcc cattcgtatc ggggacaatg ctatgattgg
     7261 tcctaattgt cagtttttga cacccctcca tccactagat ccacaggaac gcaattcagg
     7321 tatcgagtac ggaaagccta tcacaatcgg agataatttc tggactggtg gtggcgtcat
     7381 tgtccttcct ggagtgacac tgggaaataa tgtcgttgca ggagcagggg cagtaattac
     7441 caaatctttt ggcgacaacg ttgtcctagc tggcaatcct gcgcgcgtga ttaaggaaat
     7501 acctgttaaa tagaagtaaa aaggaacagc tggggttgtt tcttttttgt aggtttcatc
     7561 attttttacc cagttcacat ttacctactc tatctcttag caagtctgtt tcattaagca
     7621 agttcaaagc atctcgtaag tgggatgttt ttctcctcag ttcatcagct tcctccttga
     7681 cactcggtca gattttgata caatagtaca aaattagagg aggcaggcta tgattcagaa
     7741 acatgcgatt cctattttag agtttgatga caatcctcag gcggttatca tgcccaatca
     7801 cgaggggctg gacttgcagt tgccaaagaa gtgtgtttat gcatttttag gtgaggagat
     7861 tgaccgctat gcgagggaag taggggcgaa ctgtgttggc gaatttgttt ctgccaccaa
     7921 gacctatcca gtttatgtcg tgaactacaa ggacgaggag atttgtctgg ttcaggctcc
     7981 tgtcggttca gctccagcag cccagtttat ggattggttg attggctatg gtgtggagca
     8041 gattatctct actgggacct gtggtgtcct agctgatata gaggaaaatg cctttctagt
     8101 ccctgttcgc gctctgcgag atgaaggagc cagttaccac tatgtggcac cttctcgtta
     8161 tatggaaatg cagccagagg ctattgctgc cattgagcga gttttggatg atagagggat
     8221 tccttatgaa gaagtcatga cctggtccac agatggtttt taccgagaaa cggctgaaaa
     8281 ggtggcttat cgcaaggaag aagactgtgc tgttgtggag atggagtgtt ccgctcttgc
     8341 ggcagtagct caattgcgtg gggttctctg gggtgaattg ttgttcacag cagattctct
     8401 agcggacttg gaccagtacg acagtcgtga ctggggctcg gaagctttta ataaggcgct
     8461 agaactgagt ttagcaagtg ttcaccacct ttagttgtac tggcaaagga tttgttttat
     8521 cataaaatgt ctagctcata cttttcaaaa atatgtttaa acgaggtcac cttcctcttg
     8581 tcctaggcat gttgaggttg ggaaaaatct ttaaaatcag aaaaacgtat catatcaggt
     8641 gatgaaaact ttgacactat gcgttttatg tcgataagat ttagagtgag atgaaatgat
     8701 actcttcgaa aatctcttca aaccaggtca gcttcacctt gccgtaggta tatgttactg
     8761 acttcgtcag tcttatccgg caacctcaaa acggtgtttt gagctgactt cgtcagttct
     8821 atttgcaacc tcaaaacagt gttttgagca acctgtgact agctttctaa tcgatgcctt
     8881 ggttttcatt gcctataatc aaaaagagaa attttctcct gaaaagcata tagagtagct
     8941 ggcgttaaaa gctcctgtct tgcttttttg acctatagtc acatctatca agtattgttc
     9001 ttgcctaagc tatcaataaa aaggtggcat tttttaggct tggtgttagt agattttgcc
     9061 ttatcctatc taagtcattt cgaacttttt atggtacaat ggaaacatgt tattcaaatt
     9121 atctaaggaa aaaatagagc taggcttatc tcgtttatcg ccagcccgtc gtattttttt
     9181 gagttttgcc ttggtcattt tactaggctc tcttcttttg agcttgccct ttgtccaagt
     9241 tgaaagctca cgagcgactt attttgatca tcttttcact gctgtatctg cagtctgtgt
     9301 gacgggtctc tcaacccttc cagtagctca cacctataat atctggggtc aaataatctg
     9361 tttgctcttg attcagatcg gtggtctagg gctcatgacc tttattgggg ttttctatat
     9421 ccagagcaag caaaagctta gtcttcgtag ccgtgcaact attcaggata gttttagtta
     9481 tggagaaact cgatctttga gaaagtttgt ctattctatt tttctcacga cctttttggt
     9541 tgagagcttg ggagctattt tgcttagttt tcgccttatt cctcaacttg gctggggacg
     9601 tggtcttttt agttccattt ttctagcgat ctcagccttc tgtaatgccg gttttgataa
     9661 tttagggagc accagtttat ttgcttttca gaccgattta ctggtcaatc tggtgattgc
     9721 aggcttaatt attacaggcg gccttggttt tatggtctgg tttgatttgg ctggtcatgt
     9781 aggaagaaag aaaaaaggac gtctgcactt tcatacgaag cttgtactat tgttgactat
     9841 aggtttgttg ttatttggaa cggcaactac tctctttctt gagtggaaca atgctggaac
     9901 gattggcaat ctccctgttg ccgataaggt tttagttagc ttttttcaaa cagtgacgat
     9961 gcgaacagct ggcttttcta cgatagatta tactcaggct catcctgtga ctcttttgat
    10021 ttatatctta cagatgtttc taggtggggc acctggagga acagctgggg gactcaagat
    10081 tacgacattt tttgtcctct tggtctttgc acgaagtgag cttctaggct tgcctcatgc
    10141 caatgttgcg agacgaacga ttgcgccgcg aacggttcaa aaatccttta gtgtctttat
    10201 tatctttttg atgagcttct taataggatt gattctgcta gggataacag ccaaaggcaa
    10261 tcctcccttt atccacctca tatttgaaac catttcagct cttagtacag ttggtgtaac
    10321 ggcaaatctg actcctgacc ttgggaaatt ggctctcagt gttatcatgc cacttatgtt
    10381 tatgggacga attggtccct tgaccttgtt tgttagcttg gcagattacc atccagaaaa
    10441 gaaagatatg attcactata tgaaagcaga tattagtatt ggttaagaaa ggaaagagca
    10501 tgtcagatcg tacgattgga attttgggct tgggaatttt tgggagcagt gtcctagctg
    10561 ccctagccaa gcaggatatg aatattatcg ctattgatga ccacgcagag cgcatcaatc
    10621 agtttgagcc agttttggcg cgtggagtga ttggtgacat cacagatgaa gaattattga
    10681 gatcagcagg gattgatacc tgcgataccg ttgtagtcgc gacaggtgaa aatctggagt
    10741 cgagtgtgct tgcggttatg cactgtaaga gtttgggggt accgactgtt attgctaagg
    10801 tcaaaagtca gaccgctaag aaagtgctag aaaagattgg agctgactcg gttatctcgc
    10861 cagagtatga aatggggcag tctctagcac agaccattct tttccataat agtgttgatg
    10921 tctttcagtt ggataaaaat gtgtctatcg tggagatgaa aattcctcag tcttgggcag
    10981 gtcaaagtct gagtaaatta gacctccgtg gcaaatacaa tctgaatatt ttgggtttcc
    11041 gagagcagga aaattcccca ttggatgttg aatttggacc agatgacctc ttgaaagcag
    11101 atacctatat tttggcagtc atcaacaacc agtatttgga taccctagta gcattgaatt
    11161 cgtaaagagg gatgacccct cttttttgat gcccaagatg gcaaatagag acagaagccc
    11221 cttgtcttct agtaaaagtt cttcaaaggc tggactttat ggtaaaatag aaggaagtga
    11281 caagagagag taatactcaa tgaaaatcaa agatcaaact aggaaactag ctacgggctg
    11341 cttaaaacac tgttttgagg ttgtggatag aactgacgaa gtcagctcaa aacactgttt
    11401 tgaggttgca gatagaactg acgaagtcag taacatctat acggcaaggc gatgttgacg
    11461 cggtttgaag agattttcga agagtataag aaaaaatcag tcccctaaag gagcagatta
    11521 tgaagttatt gtctatcgcc atttctagct ataatgcagc agcctatctt cattactgtg
    11581 tggagtcgct agtgattggc tgggaccagg tcggaaattt ctccaaaggc cagtatatca
    11641 tgatgcactc gctgatttat cggacagatt tgttgcgtgc tagccagttc taattgcctg
    11701 aacatacttt ttatgtcgat aatctctttg tctttacgcc ccttcagcag gtcaagacca
    11761 tgtactatct gcctgtcgat ttctatcgtt atttgattgg gcgtgagggc cagtctgtca
    11821 atgagcaagt gatgattaag tgcattgacc agcaactcaa ggtcaatcga ctcttggtag
    11881 accagcttga tttgtcccaa gtgagtcatc ccaaaatgcg agaatatctg ctgaatcata
    11941 ttgaaatcac gacggtgatt tccagtaccc tgctcaaccg atctgaaaca gcggagcatc
    12001 tggcaaaaaa acgccaattg tggacctata ttcagcagaa aaatccagaa gtctttcagg
    12061 ctattcgtaa gaccatgttg agccgtttga ccaaacattc tgtcttgcca gatcgcaaac
    12121 tgtccaatgt cgtctatcaa atcaccaaat ctgtttatgg atttaattaa tataagtgtt
    12181 ttataagagg gatttaagaa aaattttaac tttttcttag tcctttttaa tttcaggaga
    12241 ttatactaga gtcatcaaat aaagaaagac tctaaggaga atcctatgaa attcaatcca
    12301 aatcaaagat atactcgttg gtctattcgc cgtctcagtg tcggtgttgc ctcagttgtt
    12361 gtggctagtg gcttctttgt cctagttggt cagccaagtt ctgtacgtgc cgatgtggtc
    12421 aatccgaccc caggtcaagt cttacctgaa gagacatcgg gaacgaaaga gggtgactta
    12481 tcagaaaaac caggagacac cgttctcact caagcgaaac ctgagggcgt tactggaaat
    12541 acgaattcac ttccgacacc tacagaaaga actgaagtga gcgaggaaac aaactcttct
    12601 agtctggata cactttttga aaaagatgaa gaagctcaaa aaaatccaga gctaacagat
    12661 gtcttaaaag aaactgtaga tacagctgat gtggatggga cacaagcaag tccagcagaa
    12721 actactcctg aacaagtaaa aggtggagtg aaagaaaata caaaagacag catcgatgtt
    12781 cctgctgctt atcttgaaaa agctgaaggg aaaggtcctt tcactgccgg tgtaaaccaa
    12841 gtaatccctt atgaactatt cgctggtgat ggtatgttaa ctcgtctatt actaaaagct
    12901 tcggataatg ctccttggtc tgacaatggt actgctaaaa atcctgcttt acctcctctt
    12961 gaaggattaa caaaagggaa atacttctat gaagtagact taaatggcaa tactgttggt
    13021 aaacaaggtc aagctttaat tgatcaactt cgcgctaatg gtactcaaac ttataaagct
    13081 actgttaaag tttacggaaa taaagacggt aaagctgact tgactaatct agttgctact
    13141 aaaaatgtag acatcaacat caatggatta gttgctaaag aaactgtcga aaaagctgtt
    13201 a
//
LOCUS       DAVLVP010000049        12717 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.049, whole genome shotgun sequence.
ACCESSION   DAVLVP010000049 DAVLVP010000000
VERSION     DAVLVP010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12717
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.049"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(301..1137)
                     /locus_tag="VYN69_001326"
     CDS             complement(301..1137)
                     /locus_tag="VYN69_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1533591.1"
                     /translation="MKKWMLVLVSLMTALFLVACGKNSSETSGDNWSKYQSNKSITIG
                     FDSTFVPMGFAQKDGSYAGFDIDLATAVFEKYGITVNWQPIDWDLKEAELTKGTIDLI
                     WNGYSATDERREKVAFSNSYMKNEQVLVTKKLSGITTAKDMTGKTLGAQAGSSGYADF
                     EANPELLKNIVANKEANQYQTFNEALIDLKNDRIDGLLIDRVYANYYLEAEGVLNDYN
                     VFTVGLETEAFAVGARKEDTTLVKKINEAFSSLYKDGKFQEISQKWFGEDVATKEVKE
                     GQ"
     gene            complement(1150..1779)
                     /locus_tag="VYN69_001327"
     CDS             complement(1150..1779)
                     /locus_tag="VYN69_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1533592.1"
                     /translation="MLELRNINKVFGDKQILSNFSLSIPEKQILAIVGPSGGGKTTLL
                     RMLAGLETIDSGQIFYNGQPLELDELQKRNLLGFVFQDFQLFPHLSVLENLTLSPVKT
                     MGMKQEEAEKKASGLLEQLGLGGHAEAYPFSLSGGQKQRVALARAMMIDPEIIGYDEP
                     TSALDPELRLEVEKLILQNRELGMTQIVVTHDLQFAENIADVLLKVEPK"
     gene            complement(1789..2430)
                     /locus_tag="VYN69_001328"
     CDS             complement(1789..2430)
                     /locus_tag="VYN69_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1533593.1"
                     /translation="MSYLFEILPSLLNGASTTVQVFALVLLFSIPLGVLIAFALQVHW
                     KPLHYLINIYIWVMRGTPLLLQLIFIYYVLPSIGIRLDRLPAAIIAFVLNYAAYFAEI
                     FRGGIDTIPRGQYEAAKVLKFSPFDRVRYIILPQVTKIVLPSVFNEVMSLVKDTSLVY
                     ALGISDLILASRTAANRDASLVPMFLAGAIYLILIGIVTIISKKVEKKYSYYR"
     gene            complement(2576..3805)
                     /locus_tag="VYN69_001329"
     CDS             complement(2576..3805)
                     /locus_tag="VYN69_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEV1533594.1"
                     /translation="MNNSQQMLQALEEQDLTKAEHYFAKALENDSSDLLYELATYLEG
                     IGFYPQAKEIYLKIVEDFPEVHLNLAAIASEDGQIEEAFTYLEEIQADSDWYVSSLAL
                     KADLYQLEGLTDVAREKLLEALTYSEDSLLILGLAELDSELENYQAAIQAYAQLDNRS
                     IYEQTGISTYQRIGFAYAQLGKFETATEFLEKALELEYDDLTAFELASLYFDQEEYQK
                     ATLYFKQLDTISPDFEGYEYGYSQALHKEHQVQEALRIAKQGLEKNPFETRLLLAASQ
                     FSYELHDASGAENYLLTAKEDAEDTEEILLRLATIYLEQERYEDILDLQSEEPENLLT
                     KWMIARSYQEMDDLDTAYEHYQELTGDLKDNPEFLEHYIYLLRELGHFEEAKVHAHTY
                     LKLVPDDVQMQELFERL"
     gene            complement(3795..4961)
                     /locus_tag="VYN69_001330"
     CDS             complement(3795..4961)
                     /locus_tag="VYN69_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1533595.1"
                     /translation="MEQKEKHFSLSWFFKWFLDNKAITVFLVTLLLGLNLFILSKISF
                     LFSPVLDFIAVVMLPVILSGLLYYLLNPIVDWMEKHKVNRVIAITIVFVIIALFIIWG
                     LAVAIPNLQRQVLTFARNVPVYLEDIDRIVNGLVAQHLPDDFRPQLEQVLTNFSSQAT
                     VLASKVSSQAVNWVSAFISGASQVIVALIIVPFMLFYLLRDGKGLRNYLTQFIPRKLK
                     EPVGQVLSDVNQQLSNYVRGQVTVAIIVAVMFIIFFKIIGLRYAVTLGVTAGILNLVP
                     YLGSFLAMLPALVLGLIAGPVMLLKVVIVFIVEQTIEGRFVSPLILGSQLNIHPINVL
                     FVLLTSGSMFGIWGVLLGIPVYASAKVVISAIFEWYKVVSGLYELEGEEVKSEQ"
     gene            5105..6118
                     /locus_tag="VYN69_001331"
     CDS             5105..6118
                     /locus_tag="VYN69_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000665618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEV1533596.1"
                     /translation="MKETVYFGTYTRRTSQGIYKADFDTETGQLSNLELFAAEPSPTY
                     LAFDQHQHLYTVGSQDDKGGIAAYQTDGTVLNHVVEEGAPHCYVAVDEKRDLVYAANY
                     HKGQVLVYKRQEDGSLLLSDMDQHSGQGPHENQASPHVHYTDLTPDHYLVTCDLGTDQ
                     VITYDLDQEGKLSKLYTYHSKPGAGSRHIIFHNHYKIAYLICELNSTIEVLIYDGVGE
                     FERMQVISTLPEAYEGFNGTAAIHLSKDGKYLYASNRGHDSIAVYTILADGSLELLEI
                     VPTHGQTPRDFDLTPDQKFLIVVHQDSDNATVFKRNCDNGRLAELSNDFHVPEAVCIR
                     FAP"
     gene            complement(6163..6582)
                     /locus_tag="VYN69_001332"
     CDS             complement(6163..6582)
                     /locus_tag="VYN69_001332"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEV1533597.1"
                     /translation="MAQLTVQIVTPDGLVYDHHASYVSVRTLDGEMGILPRHENMIAV
                     LAVDEVKVKRIDDKDHVNWIAVNGGVIEIANDMITIVADSAERARDIDISRAERAKLR
                     AERAIEEAQDKHLIDQERRAKIALQRAINRINVGNRL"
     gene            complement(6593..7999)
                     /gene="atpD"
                     /locus_tag="VYN69_001333"
     CDS             complement(6593..7999)
                     /gene="atpD"
                     /locus_tag="VYN69_001333"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEV1533598.1"
                     /translation="MSSGKIAQVIGPVVDVLFAAGEKLPEINNALVVYKNDERKTRIV
                     LEVALELGDGMVRTIAMESTDGLTRGMEVLDTGRPISVPVGKETLGRVFNVLGDTIDL
                     EAPFTEDAERQPIHKKAPTFDELSTSSEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYWEMKESGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSVTSIQAIYVPADDYTDPAPATAFAHLDSTTNLERKLVQ
                     LGIYPAVDPLASSSRALAPEIVGEEHYAVAAEVKRVLQRYHELQDIIAILGMDELSDE
                     EKTLVARARRIQFFLSQNFNVAEQFTGQPGSYVPVAETVRGFKEILDGKYDHLPEDAF
                     RGVGSIEDVIAKAEKMGF"
     gene            complement(8085..8963)
                     /locus_tag="VYN69_001334"
     CDS             complement(8085..8963)
                     /locus_tag="VYN69_001334"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002895995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEV1533599.1"
                     /translation="MAVSLNDIKTKIASTKNTSQITNAMQMVSAAKLGRSEEAARNFQ
                     VYAQKVRKLLTDILHGNGAGASTNPMLISRSVKKTGYIVITSDRGLVGGYNSSILKAV
                     MELKEEYHPDGKGFEMICIGGMGADFFKARGIQPLYELRGLADQPSFDQVRKIISKTV
                     EMYQNELFDELYVCYNHHVNTLTSQMRVEQMLPIVDLDPNEADEEYSLTFELETSREE
                     ILEQLLPQFAESMIYGAIIDAKTAENAAGMTAMQTATDNAKKVINDLTIQYNRARQAA
                     ITQEITEIVAGASALE"
     gene            complement(8979..10484)
                     /locus_tag="VYN69_001335"
     CDS             complement(8979..10484)
                     /locus_tag="VYN69_001335"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011889023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEV1533600.1"
                     /translation="MAINAQEISALIKQQIENFKPNFDVTETGVVTYIGDGIARAHGL
                     ENVMSGELLNFENGSYGMAQNLESTDVGIIILGDFTDIREGDTIRRTGKIMEVPVGES
                     LIGRVVDPLGRPVDGLGEIHTDKTRPVEAPAPGVMQRKSVSEPLQTGLKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQDMICIYVAIGQKESTVRTQVETLRQYGAL
                     DYTIVVTASASQPSPLLFLAPYTGVAMAEEFMYQGKHVLIVYDDLSKQAVAYRELSLL
                     LRRPPGREAFPGDVFYLHSRLLERSAKVSDELGGGSITALPFIETQAGDISAYIATNV
                     ISITDGQIFLGDGLFNAGIRPAIDAGSSVSRVGGSAQIKAMKKVAGTLRIDLASYREL
                     EAFTKFGSDLDAATQAKLNRGRRTVEVLKQPVHKPLPVEKQVTILYALTHGFLDTVPV
                     DDIVRFEEEFHTFFDAQHPEILETIRDTKDLPEEAVLDAAITEFLNQSSFQ"
     gene            complement(10499..11035)
                     /locus_tag="VYN69_001336"
     CDS             complement(10499..11035)
                     /locus_tag="VYN69_001336"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEV1533601.1"
                     /translation="MDKKTVKVIEKYSMPFVQLVLEKGEEDRIFSDLTQIKQVVEKTG
                     LPSFLKQVAVDESDKEKTIAFFQDSVSPLLQNFIQVLAYNHRANLFYDVLVDCLNRLE
                     KETNRFEVTITSAHPLTDEQKTRLLPLIEKKMSLKVRSVKEQIDESLIGGFVIFANHK
                     TIDVSIKQQLKVVKENLK"
     gene            complement(11035..11529)
                     /gene="atpF"
                     /locus_tag="VYN69_001337"
     CDS             complement(11035..11529)
                     /gene="atpF"
                     /locus_tag="VYN69_001337"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006145707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEV1533602.1"
                     /translation="MHVTVGELIGNFILITGSFILLLVLIKKFAWSNITGIFEERAEK
                     IASDIDRAEEARQKAEVLAQKREDELAGSRKEAKTIIENAKETAEQSKANILADAKLE
                     AGHLKEKANQEIAQNKVEALQSVKGEVADLTISLAGKIISQNLDSHAHKALIDQYIDQ
                     LGEA"
     gene            complement(11543..12259)
                     /gene="atpB"
                     /locus_tag="VYN69_001338"
     CDS             complement(11543..12259)
                     /gene="atpB"
                     /locus_tag="VYN69_001338"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEV1533603.1"
                     /translation="MEESINPIISIGPVIFNLTMLAMTLLIVGVIFVFIYWASRNMTL
                     KPKGKQNVLEYVYDFVIGFTEPNIGSRYMKDYSLFFLCLFLFMVIANNLGLMTKLQTI
                     DGTNWWSSPTANLQYDLTLSFLVILLTHIESVRRRGFKKSIKSFMSPVFVIPMNILEE
                     FTNFLSLALRIFGNIFAGEVMTSLLLLLSHQAIYWYPVAFGANLAWTAFSVFISCIQA
                     YVFTLLTSVYLGNKINIEEE"
     gene            complement(12294..12494)
                     /locus_tag="VYN69_001339"
     CDS             complement(12294..12494)
                     /locus_tag="VYN69_001339"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HEV1533604.1"
                     /translation="MNLTFLGLCIACMGVSVGEGLLMNGLFKSVARQPDMLSEFRSLM
                     FLGVAFIEGTFFVTLVFSFIIK"
     gene            complement(12569..>12717)
                     /locus_tag="VYN69_001340"
     CDS             complement(12569..>12717)
                     /locus_tag="VYN69_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001153868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1533605.1"
                     /translation="KEVAFIEKWINNYPKKCLDYKSPREDFLMANLNLKFSKMEIIFI
                     KRFQ"
BASE COUNT         3892 a         2817 c         2297 g         3711 t
ORIGIN      
        1 acgcaccaag ggaggagtct gcccttttga ggaaatctag cgaggaaaaa cgatactgga
       61 acagcagaaa gtaaaactga cctcatgagg aggaagaaag tggctcatga ggtcaggggt
      121 ttggtaagtt acatctagtt gagagaggta tgaatgattt gggtttaaca atgttttaga
      181 aatcacagtg ggctattcca tattcaatta tccagattca atcgctatta tcgttttcct
      241 agattacaaa aaaaaactac gttttgctaa tcaaaacgca gtcttagcca ctattttatc
      301 ttactgtcct tcttttactt ctttggttgc tacatcttct ccaaaccatt tttggctgat
      361 ttcttggaac ttgccgtcct tgtaaagact agaaaaagct tcatttatct tcttaaccaa
      421 ggttgtatct tccttacggg ctccaaccgc aaaagcttct gtttctagtc caactgtaaa
      481 gacattataa tcgtttaaaa caccttctgc ttctaaataa tagtttgcat agacacggtc
      541 aatcaataga ccatcaattc gatcgttttt caaatcaatc aaggcttcat taaaggtttg
      601 gtattgattc gcttccttat tagcgacaat attcttcaaa agttctggat ttgcttcaaa
      661 gtccgcataa ccagatgaac cagcttgagc tcctaatgtc tttccagtca tatcctttgc
      721 agtcgtgata ccagataatt tcttcgtaac caatacctgc tcattcttca tatatgagtt
      781 actgaaagcc accttttcac ggcgttcgtc tgtagcggaa tagccattcc aaatcagatc
      841 aatcgttcct tttgtcaatt cagcttcttt caaatcccaa tcaatcggtt gccaatttac
      901 cgtgattccg tatttttcaa aaacagctgt agctaaatca atatcaaatc ctgcataaga
      961 accatctttc tgagcaaatc ccattggaac aaaagtacta tcaaatccaa tagtaataga
     1021 cttgttagac tggtactttg accaattatc tccactagtt tcgctagaat ttttcccaca
     1081 agctactaag aacaaagcag tcatcagact gactaataca agcatccatt ttttcatcca
     1141 tttttcctcc tatttaggtt ctactttcaa taatacatct gcgatatttt cagcaaactg
     1201 caaatcatgg gtaaccacaa tctgggtcat cccaagttcc ctattttgca agattagctt
     1261 ctccacttcc aaacgtaatt ctggatccag ggcagaagtt ggttcatcgt agccaatgat
     1321 ttctgggtca atcatcatag cacgcgccaa agccactcgc tgcttttgcc caccagatag
     1381 tgagaaagga taggcctctg cgtgtcctcc tagtcctaac tgttccaaga gtccactcgc
     1441 cttcttctca gcctcttcct gcttcattcc catggtcttc acaggcgata aagtcaaatt
     1501 ttccagaact gatagatgag gaaatagttg aaaatcttgg aagacaaatc ccagtaggtt
     1561 gcgcttctgc aattcatcca gctctaaagg ttgtccatta taaaagattt gccctgaatc
     1621 aatggtttca agacctgcaa gcatacgtaa aagagttgtc ttacctccac cagaaggtcc
     1681 aacgatagcc aggatttgct tttcaggaat acttagactg aaattagaca ggatttgttt
     1741 gtctccaaag actttattga tatttcgtaa ttctaacatg gcagcctcct atctataata
     1801 actatacttc ttctcaactt ttttggaaat aattgtcaca atcccaatca aaatcaaata
     1861 aatggctcct gccaagaaca taggaaccag actagcatcg cggttagcag ctgttcgact
     1921 agccaagata aggtctgaaa ttccgagagc atagaccaaa gaagtatcct tgaccaaact
     1981 cataacttca ttaaagacac taggaagaac gatcttggtc acttggggca agataatata
     2041 gcgcactctg tcaaaagggc taaacttcaa gaccttggca gcctcatact gtcctcttgg
     2101 aatagtgtca attcccccac ggaaaatttc tgcaaagtaa gccgcataat tcaaaacaaa
     2161 ggcaataata gctgcaggaa ggcggtctaa acgaatccca atacttggga gcacataata
     2221 gataaaaatc agttgcaaga gtaagggggt tcctcgcata acccagatgt aaatgttaat
     2281 cagataatgg aggggcttcc aatggacttg caaggcaaag gcaatcaaaa cgcccaaggg
     2341 aatcgaaaat agcaagacca gtgcaaagac ctgtacagtc gtgctcgcac cattcagtaa
     2401 actcggtaat atctcaaata aataagacat actgcacctc ctaaaagtaa ttgttcctat
     2461 tatagcatga ataaacaaga ttaccaagta tttttgtaaa aagattctac catatacaaa
     2521 taaaaaccgt agctatcaaa agagataaac tacagttcta tatgtttaaa cattcttaca
     2581 atctctcaaa cagttcttgc atttgcacat catctggaac cagttttaag taagtgtgag
     2641 catggacttt tgcttcttca aaatgtccca attcacgcaa gagatagata tagtgttcca
     2701 gaaattctgg attgtccttc aaatctcctg tcaactcttg ataatgctca taagcagtat
     2761 ccaaatcgtc catttcttga taagaacgag caatcatcca cttggtcaaa agattttctg
     2821 gctcctcact ctgcaagtct agaatatcct cataacgctc ctgctccaga taaatagtgg
     2881 ctaaacgaag caagatttct tctgtatcct cagcgtcttc ttttgcagta aggagataat
     2941 tttctgcacc actagcatca tgcaattcat aagaaaattg tgaagcagct agcaagaggc
     3001 gagtttcaaa gggatttttc tctaatcctt gcttagcgat acgcagggct tcttgaactt
     3061 gatgttcctt atgtaaagcc tgactgtacc catactcata gccttcaaag tcaggagaaa
     3121 tggtatcaag ctgcttaaag tagagggtgg ctttttgata ttcttcttga tcaaaataaa
     3181 gactggccaa ctcaaaagct gttaagtcat cgtattctaa ctccagggct ttttctaaaa
     3241 actcagtagc cgtttcaaat ttccctaact gagcataggc aaagccaatt cgttgatagg
     3301 tggaaatgcc cgtttgctca taaatcgagc gattatctaa ctgggcatag gcttgaatag
     3361 ccgcttggta attttccaac tcactatcca actctgccaa acccaatatc aagagagaat
     3421 cctctgagta ggtcaaggcc tccaataatt tctcacgtgc cacatctgtc aaaccttcca
     3481 gctggtaaag gtctgccttc agagccaaag acgagacata ccagtcactg tcagcttgga
     3541 tttcctcaag ataggtaaag gcttcttcta tttgaccatc ctcgctagca attgcagcta
     3601 gattaagatg aacctctgga aaatcctcta caattttcag gtaaatttcc ttggcctgag
     3661 gatagaaacc aatcccttca agataagttg ccaactcata cagaagatca cttgaatcat
     3721 tttctaaagc tttggcgaaa taatgctcag ccttagttaa atcttgctcc tccaaagcct
     3781 gtaacatctg ttgactattg ttcactcttg acttcctcac cctctaattc atatagacca
     3841 ctgactacct tataccattc gaaaatggct gaaatgacaa ccttagcaga ggcataaacc
     3901 ggaataccaa gtaaaactcc ccagatacca aacatagatc ctgaagttaa caaaacaaag
     3961 agaacattaa taggatggat gtttaattga cttcccaaaa tcaatggaga gacaaaacgg
     4021 ccttcaatag tttgttctac aataaagaca atcactactt tcaaaagcat gactggacca
     4081 gcaatcaaac ccaatactag ggcaggaagc atggctagaa agctaccaag ataagggacc
     4141 agatttaaaa taccagcagt aacccccagc gtaaccgcat agcgtagacc aataatcttg
     4201 aagaagatga taaacattac tgctacaata atagccactg tcacttgccc tcgaacataa
     4261 ttggacaact gttgattcac atctgataga acttgtccaa caggttcctt caattttctt
     4321 ggaatgaatt gggtcaaata gttacgcaag cctttcccat cacgcaagag ataaaagagc
     4381 atgaaaggaa cgataatcaa ggcaacaatc acttgagaag ctccgctaat aaaggcactc
     4441 acccagttga ctgcctgaga tgaaacctta cttgccaaaa ctgtagcctg gctagaaaaa
     4501 ttggtcaaaa cttgctctaa ttgaggtctg aaatcatctg gcaggtgctg ggctaccaat
     4561 ccattaacaa tcctatctat atcttctaag taaacaggaa cgtttcttgc aaaggtcaaa
     4621 acctgacgtt gcagatttgg aatggcgact gccaagcccc aaatgataaa gagagcgatg
     4681 ataacaaaga caatagtgat agctataaca cgattaacct tatgcttctc catccaatca
     4741 acaataggat tcaacaaata atataacaaa ccagacaaaa tgactggcaa catcacaact
     4801 gctataaagt ctaaaacagg tgaaaataga aaactaatct tacttaaaat aaaaagattc
     4861 aatcccaata ataaggttac taaaaatacc gtaattgcct tgttatctaa aaaccacttg
     4921 aaaaaccaag ataggctaaa atgtttctct ttttgctcca taaatacttc ctttctattg
     4981 ttctctcatt ataccatttt tgaaacaaaa taactctttt ttaaagtgct tacatctaat
     5041 tgaatgtctt taatttgctt gtttttgtga tataatagtc ttatctttac acagaggtat
     5101 agtaatgaaa gaaactgttt attttggaac ttatacacgt cgtacttctc aagggattta
     5161 caaggcagac tttgatacag aaactggtca gctttcaaat ctagaacttt ttgcagctga
     5221 gccaagtcca acctaccttg cctttgacca gcaccaacat ttatacactg ttggtagcca
     5281 agacgataag gggggaattg cagcctatca aactgacggg actgtgttaa atcatgttgt
     5341 tgaagaagga gctccccact gttatgttgc tgtcgatgaa aagcgtgatt tggtttacgc
     5401 agctaactat cacaagggac aagtccttgt ttataaacgc caggaagatg gtagtcttct
     5461 acttagtgat atggatcaac acagtggcca aggtccacat gaaaatcaag cttcccccca
     5521 tgttcactat acagatttaa cacctgacca ctatctagtg acctgcgact tgggaactga
     5581 ccaagtcatc acctatgacc tcgatcaaga aggaaaatta tctaagctct atacctatca
     5641 cagcaagcca ggagcaggct cacgccatat cattttccat aaccactata aaatcgctta
     5701 tctcatttgt gaactcaata gtactatcga agttttaatc tacgatggcg ttggcgaatt
     5761 tgaacgtatg caggtcattt caactttacc ggaagcttac gaaggcttta atggaaccgc
     5821 tgctattcat ctctctaaag acggtaaata cctctacgct tctaaccgtg gccatgattc
     5881 tatcgcagta tataccatcc ttgcggacgg tagcttagag ttattagaaa tcgttccaac
     5941 gcatggtcag actccacgtg attttgattt gacacccgac caaaaatttc tcattgttgt
     6001 ccatcaagac tctgacaatg caactgtctt taaacgtaat tgtgacaatg gtcgtctagc
     6061 agaactctcc aacgacttcc atgttcccga agcagtctgc atccgttttg ctccttaaaa
     6121 caccataaaa aatgaacttg gtattttcaa gttcattttt tcttatagtc tatttccgac
     6181 attaatacgg ttaatggcac gttgcaaagc aatcttagca cgacgttctt ggtcaatcaa
     6241 atgtttgtct tgtgcttctt caattgcacg ttctgcacga agtttggcac gttctgcacg
     6301 actgatatcg atatcacgag cacgttctgc agagtcagcg acgattgtga tcatatcatt
     6361 ggcaatttca ataacgccac cgtttactgc aatccagttc acgtgatctt tatcatcgat
     6421 acgttttacc tttacttcat caactgctaa aaccgcaatc atattttcat gtcgtggcaa
     6481 gatccccatc tcaccatcca gagttcgaac cgatacatag ctggcatggt gatcatagac
     6541 gagaccatct ggtgtcacga tctggacagt taactgagcc atagatcacc tcttaaaatc
     6601 ccattttttc agcttttgca atcacatctt cgatagaacc tacaccacgg aaggcatctt
     6661 ctggcaagtg gtcgtattta ccatcaagga tttccttaaa gccacgtaca gtttcagcaa
     6721 ctggaacata agaacctggc tgaccagtaa attgttccgc aacgttgaag ttttgtgaca
     6781 agaagaactg gatacgacgg gcgcgagcaa ccaaggtctt ttcttcatca gaaagctcat
     6841 ccataccaag gatagcaatg atatcttgca attcatggta acgttgaagg acacgtttta
     6901 cttcagcagc aactgcatag tgctcttctc caacgatttc aggtgccaag gcacgtgagc
     6961 ttgaagcaag tgggtcaacg gctgggtaga tacccaattg taccaactta cgttccaagt
     7021 ttgttgttga atccaagtga gcgaaggctg ttgctggcgc tgggtcagta tagtcatccg
     7081 ctggcacata gatagcctgg atagaggtta cagaaccctt cttggttgat gtgatacgtt
     7141 cttgcaattg acccatttcc gtagcaagtg ttggttggta accaacggct gatggcatac
     7201 gacccaaaag ggcagatact tctgaaccag cctgagtgaa acggaagata ttatcgataa
     7261 agagaagcac gtcttggcct tccacatcac ggaagtattc agcgattgtc aaaccagtaa
     7321 gggcaacacg catacgtgct cctggtggct cattcatctg accaaagacc atggctgttt
     7381 tctcgataac gcctgattct ttcatttccc agtaaaggtc attcccctca cgagtacgtt
     7441 ccccaacacc agtaaatact gaaataccac cgtgctcttg ggcaatgttg tgaatcaatt
     7501 cttggattaa gacggtttta ccaactccgg caccaccgaa aagtccaact ttaccacctt
     7561 taaggtaagg ggcaagaagg tcaataacct tgatccctgt ttcaaggatt tcagaagagg
     7621 tagacaactc atcaaaagtt ggagcttttt tatgaattgg ctgacgctct gcgtcttctg
     7681 taaaaggagc ttccaaatca atggtatctc ccaaaacgtt gaagacacgt cccaaagttt
     7741 ctttacctac tggtacagag attggacgac ctgtgtccaa tacttccatt ccacgagtca
     7801 acccatctgt tgattccatg gcgatagtac gaaccatacc atctcctaac tccaaggcta
     7861 cttcaaggac gattcttgtt tttctttcgt catttttgta gacgacaagt gcattgttaa
     7921 tctcaggaag tttttcccct gctgcaaaca aaacgtctac aacgggaccg ataacctgag
     7981 caattttacc tgaactcatc tccttctcct attctatata agatactgtc ggttcctagc
     8041 tcaactaggt cctaagttca ttttcatacg agctggacta gagcctattc taaggcacta
     8101 gcacctgcta cgatttctgt aatttcttgt gtaatcgccg cctgtctggc acggttatac
     8161 tgaattgtca aatcattgat gactttctta gcattatctg tcgctgtttg catggctgtc
     8221 atacccgcag cattctcagc tgtcttggca tcgataatgg caccgtaaat catactttct
     8281 gcaaactgag gcaacaactg ctccagaatt tcttctcggc tggtttccaa ttcaaaagtc
     8341 aagctgtact cttcatccgc ttcatttgga tccaagtcaa caatcggaag catttgttcc
     8401 acacgcattt gactggttag cgtattgaca tggtggttgt agcaaacata aagctcatca
     8461 aagagttcat tttggtacat ttcaacagtt tttgaaataa tcttacgaac ttgatcaaag
     8521 ctaggttggt ctgccaagcc acgtaattca taaagtggtt gaataccgcg agccttaaag
     8581 aaatcagctc ccatcccacc gatacagatc atttcaaaac ctttaccgtc tgggtggtat
     8641 tcttctttca actccataac agctttcaaa atagaggaat tataacctcc aaccaaaccg
     8701 cggtctgaag tgataacgat atagcctgtc ttcttcacag aacggctaat caacatcgga
     8761 ttagttgaag caccagctcc attaccatga aggatatctg tcaaaagttt acgcactttc
     8821 tgagcgtaaa cttggaagtt gcgagcagct tcttcagaac gacctagctt agcagccgat
     8881 accatttgca tggcattagt gatttgactc gtattttttg ttgaggcgat ttttgtttta
     8941 atatcattta gagatactgc catctgacac ctctattctt attggaagct agattgattg
     9001 agaaactctg taatcgcagc atccaagact gcttcttctg gcaagtcttt tgtatcacga
     9061 atggtttcca aaatctctgg atgttgagca tcaaagaagg tatggaactc ttcctcgaaa
     9121 cgaacaatat catctactgg aacagtatcc aagaaaccat gtgtcaaagc ataaagaatg
     9181 gttacttgtt tctcaacagg taatggtttg tgaacaggtt gtttcaagac ctcaacggta
     9241 cgacgtccac ggttcaactt agcctgtgtt gctgcgtcca agtcagaacc aaacttagta
     9301 aaggcttcca actcacggta tgaagcaagg tcgatacgaa gtgtaccagc aaccttcttc
     9361 atggctttga tttgtgcaga accacctaca cgagatacag atgaacccgc atcgatggct
     9421 ggacgaatac ctgcattgaa gaggccatcg ccaaggaaga tttgtccatc agtgatagaa
     9481 atcacgttgg ttgcgatata ggctgagata tctcctgctt gtgtctcgat aaatggtagg
     9541 gctgtaattg atccaccacc aagttcatca gaaactttag ctgagcgctc aagcaaacgg
     9601 ctgtggagat agaaaacatc ccctgggaag gcttcacgac ctggaggacg acgaagcaag
     9661 agcgacagtt cacgataagc taccgcttgt tttgatagat catcgtatac aatcaaaaca
     9721 tgcttacctt gatacataaa ttcttccgcc atagcaaccc cagtataagg agctaggaag
     9781 agcaatggag atggttgtga agcagaggct gtcacaacga ttgtgtagtc caaggcaccg
     9841 tactgacgaa gtgtttctac ttgcgtacga actgttgatt ctttttgtcc aatcgcgacg
     9901 tagatacaga tcatatcttg atctttttgg ttcaagattg tatcaatcgc aatggttgtt
     9961 ttccctgtct gacggtcacc gataatcaac tcacgttgac cacgaccaat cggtacaagg
    10021 gcgtcaatag ctttcaaacc agtttgcaat ggttctgaaa cagacttacg ttgcataaca
    10081 ccaggagctg gtgcttctac tggacgagtt ttatcagtgt ggatttctcc aagaccgtca
    10141 actggacgac caagcggatc cacaacacga ccaatcagac tttcacctac agggacttcc
    10201 atgattttcc ctgtacggcg gattgtatcg ccttcacgga tatctgtaaa gtcacctagg
    10261 atgataatac caacgtctgt tgactccaag ttttgagcca taccataaga gccgttttca
    10321 aaattcaaca actctccact catgacattt tcaaggccat gagcacgcgc gataccgtcc
    10381 ccgatatagg ttacaacacc tgtttcagtc acatcaaaat tgggtttgaa attttcaatt
    10441 tgttgcttaa ttaaagcgct gatttcttgt gcgttaattg ccaaaagaac accactttct
    10501 atttcaaatt ttctttaaca actttaagtt gttgtttaat actcacatca attgtcttgt
    10561 gattggcaaa aatgacaaaa ccaccaatga gactttcatc gatttgttct tttacactcc
    10621 ttactttcag agacattttt ttctcaatca aagggagcaa acgagtcttc tgttcatcag
    10681 ttagaggatg agcagacgta atcgtcactt caaatcgatt tgtttctttt tcaagtcggt
    10741 tcaagcaatc tacaagcaca tcataaaaaa gatttgctct gtgattgtag gccagaacct
    10801 ggataaagtt ttgtaataaa ggcgacacag aatcttggaa aaaagcaatt gttttttcct
    10861 tatccgactc gtctactgcc acttgtttta aaaaagaagg cagacctgtt ttttcaacaa
    10921 cttgcttgat ttgagtcaag tctgaaaaga tacggtcttc ttctcctttt tcaagtacca
    10981 attggacaaa aggcatgctg tatttttcaa ttacctttac tgttttcttg tccattaagc
    11041 ttctcctagc tgatcgatat actgatcaat gagtgcttta tgggcatgac tgtcaaggtt
    11101 ttgtgagatg attttaccag ctaagctgat ggtcaaatct gcgacctcac ccttaacact
    11161 ctgtaaagct tctactttat tttgagcaat ttcttgattg gctttttctt ttaagtgtcc
    11221 tgcttctagt ttagcatctg ctaagatatt agccttactt tgctcagctg tttcctttgc
    11281 attttcaatg attgtcttag cttctttacg gctaccagcc aattcatctt cgcgtttttg
    11341 agccaatact tctgcttttt gacgggcttc ttcagctctg tcaatatctg aagcaatttt
    11401 ttcagctctt tcttcgaaaa tgcctgtaat attagaccat gcaaattttt taatcaagac
    11461 tagcaaaaga ataaaagagc cagtgattaa aataaaatta ccaattaatt cacctactgt
    11521 tacgtgcatc agttactcct ttctattcct cttcaatatt aatcttattc cctaaataca
    11581 cagatgtcaa aagagtaaaa acataagctt ggatgcagga aataaagaca gaaaatgcag
    11641 tccaagccaa attagctcca aaggctactg gataccaata aatagcttgg tgggaaagaa
    11701 gaagtaacaa actcgtcatg acctctcctg caaagatatt cccaaaaatc cgcaaagcca
    11761 aagataagaa gtttgtaaat tcttccaaga tattcatcgg tatgacaaaa acaggactca
    11821 taaaagattt tatacttttt ttaaatccac gacgacgaac gctttctata tgtgtcaaca
    11881 aaatgacaag aaaagataag gttaagtcat actgtaaatt agcggttggc gaactccacc
    11941 agttagtccc atcgatcgtt tgaagctttg tcattaagcc aaggttattg gcaatcacca
    12001 tgaaaaggaa taaacaaagg aaaaagagtg agtaatcttt catgtagcgc gaaccaatgt
    12061 taggttctgt aaatccaata acaaagtcat agacatactc aagtacattt tgctttcctt
    12121 tgggtttcaa ggtcatattg cggcttgccc aataaataaa gacaaaaata actcccacaa
    12181 tcaacaaagt catggctaac atagtcagat tgaagataac aggaccaata gagatgattg
    12241 gattaatact ttcttccatc taaaatcctc ccttttcttc tcgttccatg tatttatttg
    12301 ataataaatg agaagacaag agttacaaag aaagttcctt caataaaggc aacacctaaa
    12361 aacatcaaac tacgaaactc agaaagcata tctggttggc gtgctactga tttaaacagt
    12421 ccattcatca ataaaccttc accgacagat acgcccatac aggcaataca taagcctaaa
    12481 aatgttaaat tcataacaaa ttctccttta aaataaaatt tacttactta gtgtagtcct
    12541 aaaatttaaa tttgtcaact tttaaccgct attggaagcg tttaataaaa attatttcca
    12601 ttttgctaaa tttcaagttc aagttagcca tcaagaagtc ttctctgggt gacttgtagt
    12661 ccaagcattt tttaggatag ttgttaatcc acttttcgat gaatgcgact tctttgg
//
LOCUS       DAVLVP010000050        12686 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.050, whole genome shotgun sequence.
ACCESSION   DAVLVP010000050 DAVLVP010000000
VERSION     DAVLVP010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12686)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12686)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12686
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.050"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(155..325)
                     /locus_tag="VYN69_001341"
                     /pseudo
     CDS             complement(155..325)
                     /locus_tag="VYN69_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040325.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
     gene            complement(468..614)
                     /locus_tag="VYN69_001342"
     CDS             complement(468..614)
                     /locus_tag="VYN69_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533606.1"
                     /translation="MKEANKNHPAGAPTFAKGEGEHANDIVATYSDGTTYYVPLNDVT
                     KYAR"
     gene            complement(611..1066)
                     /locus_tag="VYN69_001343"
     CDS             complement(611..1066)
                     /locus_tag="VYN69_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533607.1"
                     /translation="MQILCYFTITVVAKPNNSGEVHLDVSIEDNQGGSGYNFSSVSSS
                     SQTAKYEGTVYNNNSSLYITIDKTSDATALLKLKLNNVDNQPATEVPSSGITVKLNAK
                     DNAGNWTSASNKKEITVKIVSAKPTYPDKILVKNPDNIKDTEKMPLLKN"
     gene            complement(1203..2087)
                     /locus_tag="VYN69_001344"
     CDS             complement(1203..2087)
                     /locus_tag="VYN69_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PucR family transcriptional regulator"
                     /protein_id="HEV1533608.1"
                     /translation="MRAKELQDWFPEARISDQPVEKEGYLTLPLASQQWILLEEAGLS
                     EREKQLVALLTQQEQARSLNPWYSYLVEGKGQAPQVFKKIQLVYCHLSYFQQENLASW
                     LDMMRTLFPNCQTVLQVGAQDYVFVLQQDKYTSVRDILSDTIEAVEYDFGLRLSIMLG
                     QVWSQTGHQALSDLIKAERDLFKTWWRQGHQGVHTFSQLYLWSMGERLVDLKPIKECL
                     HQMILDQDQIQEIILSLWENSAVLTKTAQQLYLHRNSLQYKIDKWEELTGLQLKELTD
                     LTLCYQLILGSLSTIVGL"
     gene            complement(2091..2477)
                     /locus_tag="VYN69_001345"
     CDS             complement(2091..2477)
                     /locus_tag="VYN69_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEV1533609.1"
                     /translation="MIDVNLINGIALAFEGDAVYSMYIRRHLILKGMTKPNKLHQEAT
                     KYVSAKAQARLIALMLEEQVLTEKEEEIYKRGRNTNSHTKAKNADVVTYRMSTGFEAV
                     MGYLHMTENLERLESLVSWCIQKVEG"
     gene            complement(2470..3813)
                     /locus_tag="VYN69_001346"
     CDS             complement(2470..3813)
                     /locus_tag="VYN69_001346"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002916477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEV1533610.1"
                     /translation="MIKIYDTMSRDLREFVPIEDGKIKMYVCGPTVYNYIHVGNARST
                     VAFDTIRRYFEYRGYKVAYISNFTDVDDKIINRAREEGITPQEVADKYIAAFREDVTA
                     LGVKPATRHPRVVEFMADIIRFVEDLIEKGFAYESQGDVYFRVEKSHNYAKLANKTLE
                     DLELGASGRTDEETARKENPVDFALWKSSKPGEISWDSPWGPGRPGWHIECSVMSTEI
                     LGDTIDIHGGGADLEFPHHTNEIAQSEAKTGKAFANYWMHNGFVNIDNVKMSKSLGNF
                     ITVHDALKTLDGQVLRFFFATQHYRKPINFTEKAVRDAETNLKYLKNTYEQPFTGNVD
                     AQELQNFKDKFVAAMDEDFNAANGITVVFEMAKWINSGNYDASVKQALADMLEIFGIV
                     FVEEVLDAEIEDLIQKRQEARANRDFATADQIRDQLVTQGIKLLDTKDGVRWTRD"
     gene            complement(3895..4779)
                     /locus_tag="VYN69_001347"
     CDS             complement(3895..4779)
                     /locus_tag="VYN69_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1533611.1"
                     /translation="MIQARNKLSQEELSEAKKVINCCQNYDGTYRDPYLSNMLNFDPN
                     MPAFFLYYEKGELVGLLTVYADDQDVEVTILVHPGHRRQGIARALFTSFERETASFPI
                     RSVTFQTERIFLENHPDFASNWGLIEDEETETWLGKDRRPYPLANVSHLEVLLADSSY
                     QDQISQLKFQAFSEEHESREVVDRYVAEALKDPESRLYILLKAGQVIGTCTVDLSTNT
                     NYLYGLAILEPERGKGYGSYLAKSLVNQLIEQNDKEFQIAVEDSNVGAKRLYEKIGFV
                     KQTQVVYLNEKGARDSEV"
     gene            complement(4791..5408)
                     /gene="cysE"
                     /locus_tag="VYN69_001348"
     CDS             complement(4791..5408)
                     /gene="cysE"
                     /locus_tag="VYN69_001348"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000539958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEV1533612.1"
                     /translation="MGWWRETIDIVKENDPAARTTLEVLLTYPGVKALAAHRLSHFLW
                     KHGFKLLARMYSQFWRFWTQIEIHPGAQIDSGVFIDHGSGLVIGETAIVEKGVLLYHG
                     VTLGGTGKDCGKRHPTVRKGALISAHAQVIGPVEIGENAKVGAAAVVVADVPSDVTVV
                     GIPAKIVRLHGKKDEPVIHEVEEKREYYVNKLEQAKDASHRSSGL"
     gene            complement(5424..7637)
                     /gene="pnp"
                     /locus_tag="VYN69_001349"
     CDS             complement(5424..7637)
                     /gene="pnp"
                     /locus_tag="VYN69_001349"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001854499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEV1533613.1"
                     /translation="MAKQVFQTTFAGRELIVETGQVAKQANGSVVVRYGESTVLTAAV
                     MSKKMATGDFFPLQVNYEEKMYAAGKFPGGFMKREGRPSIDATLTARLIDRPIRPMFA
                     EGFRNEVQVINTVLSYDENASAPMAAMFGSSLALSISDIPFDGPIAGVQVGYVDGQII
                     INPSQEQAEQSLLELTVAGTKHAINMVESGAKELSEEIMLEALLKGHEAVKELIAFQE
                     EIVAAVGKEKAEVELLHVDAELQAEIIAAYNSDLQKAVQVEEKLAREAATQAVKDQVT
                     AVYEEKYADHEEFDRIMRDVAEILEQMEHAEVRRLITEDKVRPDGRKVDEIRPLDAVV
                     DFLPRVHGSGLFTRGQTQALSVLTLAPMGETQIIDGLDPEYKKRFMHHYNFPQYSVGE
                     TGRYGAPGRREIGHGALGERALAQVLPSLEEFPYAIRLVAEVLESNGSSSQASICAGT
                     LALMAGGVPIKAPVAGIAMGLISDGNNYTVLTDIQGLEDHFGDMDFKVAGTRDGITAL
                     QMDIKIQGITAEILTEALAQAKKARFEILDVIEATIPEVRPELAPTAPKIDTIKIDVD
                     KIKIVIGKGGETIDKIIAETGVKIDIDEEGNVSIYSSDQDAINRAKEIIAGLVREAKV
                     DEVYRAKVVRIEKFGAFVNLFDKTDALVHISEMAWTRTNRVEDLVEIGDEVDVKVIKI
                     DEKGRIDASMKALLPRPPKPEHDEKGEKSERPHRPRHQKDYKPKKEFTETSKDSE"
     gene            complement(8423..9289)
                     /gene="metF"
                     /locus_tag="VYN69_001350"
     CDS             complement(8423..9289)
                     /gene="metF"
                     /locus_tag="VYN69_001350"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000089969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HEV1533614.1"
                     /translation="MSRQTPSLSFEVFPPNPAVGNGNIISALQYMQELAPHFISVTAS
                     NNKFNIKETTVRLADFIQNDLAIPTIAHLPAIYLTKDKVAETIADLDKVGVQKILALR
                     GDIIPDVEPQKDFRYATDLIELIKERAPHFDIIGACYPEGHPDSPNQISDIQNLKKKV
                     DAGCSSLVTQLFFDDERFYDFQDKCILAGIDVPIHAGIMPILNRNQALRLLKTCENIH
                     LPRKFKAILDKYEHDPESLRAAGLAYAVDQIVDLVTQDVAGVHLYTMNNADTAKYIHQ
                     ATHALFNHQSLG"
     gene            complement(9353..11602)
                     /gene="metE"
                     /locus_tag="VYN69_001351"
     CDS             complement(9353..11602)
                     /gene="metE"
                     /locus_tag="VYN69_001351"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEV1533615.1"
                     /translation="MSTTIIGFPRLGEFRELKFTTEKYFRKEISEEELLAAAKDLRAK
                     HWNIVKEKGITEIPSNDFSHYDNFLDAAFLFNVVPASVQNLDLSDLERYFALGRGYQG
                     EKGDVRALPMKKWFNTNYHYIVPKFEKDTQVKLAGHKIFDEFQEAKELGLNTRPVLVG
                     PFTFLQLSDFEEGVKADDFVDSLVAAYQEVFAKLAELGASRIQLDEAALVKDLTAEEK
                     ALFLNLYNKLLADKKGLEVLLQTYFGDVRDVYADLVNLPVDAIGLDFVEGKKTLELVK
                     GGFPADKTLYVGIVNGKNIWRNNYEKSLAVLEQIPAENIVLTSSCSLLHVPFTTANEE
                     FEPALLNHFAFAVEKLDEIRDLDAIRNGQGSEALAANKELFATERVGENAELRARIAG
                     LTDADYTRLPVFAEREAIQEEAFKLPALPTTTIGSFPQTKEVRAKRLAYRKGELSQKE
                     YDAFLAETIDEWIKWQEDIDFDVLVHGEFERNDMVEYFGQNLSGYLFSKNGWVQSYGM
                     RGVKPPIIWGDVTRLNPITVKWSSYAQSRTNKPVKGMLTGPVTILNWSFPREDISIKD
                     STLQIALAIKDEVLDLEAAGVKIIQIDEAALREKLPLRRSDWYEDYLDWAIPAFRLVH
                     STVAPDTQIHTHMCYSEFTDIIPAIDNMDADVISFEASRSNLEILDELKAKNFQTEVG
                     PGVYDIHSPRVPNEGEIDNTIEAILAKVPSKKVWINPDCGLKTRGIPETKESLIRLVE
                     AAKAAREKL"
     gene            complement(11862..12128)
                     /locus_tag="VYN69_001352"
     CDS             complement(11862..12128)
                     /locus_tag="VYN69_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1533616.1"
                     /translation="MEGCLSELFSKEEILESDMRVAIMSELIEARNKQGISQKKLEEV
                     SGVSQPVIARMETGKTSPQLDTVLKVLASLGKILAVVRLEQGKS"
     gene            complement(12213..12686)
                     /locus_tag="VYN69_001353"
                     /pseudo
     CDS             complement(12213..12686)
                     /locus_tag="VYN69_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
BASE COUNT         3537 a         2852 c         2476 g         3821 t
ORIGIN      
        1 caaggactgg ttttgatata gcacgtttaa gtaccgactt ctgagatact atagtagatt
       61 gaaactagaa taatatacct ctacttctaa aatattgtta gaaatcgatt tgacttccct
      121 gatcgattcg tcctattctt atttcatttt aatataattg atagtggtcg ccccagccag
      181 ataccttatc tgctatccat ttaggaaccc ctaacttaag caatccccat aatcgtctcg
      241 atttcttctt ccattgcttc cagataatca ctcgtaggcg agtacgcaag cgctcatcta
      301 tgctagtgac tatacttttc atatttataa ttcattcctt tcgtttcact caaggcacaa
      361 cacagaatga aaaagtgttg tgatctttat tttgttttat aataatagtg agaaagccta
      421 tcactactac aaatcacggg gaggtgaata agtgagtggt acagccacta cctcgcatat
      481 tttgtcacat catttaacgg tacataataa gttgtaccat ctgaataagt tgctacaata
      541 tcatttgcat gctctccttc acctttagca aaggttggag ctcctgctgg atgattttta
      601 tttgcctctt tcaatttttc aataatggca ttttttctgt atcttttata ttatcaggat
      661 ttttcactaa gattttgtct ggatatgtcg gtttagcaga aacaattttt actgttattt
      721 cttttttatt cgaagcactt gtccagtttc cagcattatc tttagcattt aattttacag
      781 taattcctga actaggaact tcagtagcag gttgattatc aacattattc aactttaatt
      841 tcaaaagagc tgttgcatca gacgttttat caatcgttat atataatgat gaattgttat
      901 tataaacagt tccttcatat ttagctgttt gtgagctact tgaaacagaa ctgaaattat
      961 acccactacc tccctgatta tcttcaatgc ttacgtctaa atgaacttcc ccactattat
     1021 ttggcttagc aacaactgtt atagtaaaat aacataaaat ttgcataaat agattaggga
     1081 aatcaaagca gcttctagga atgttttagc agtcacagtg tactttccca gcatcaagcc
     1141 actataactc tgcacataaa aatggagaag atggcaatcc tcttctccaa atattaactt
     1201 ctttacaaac caactatagt tgacaaagaa cctaaaatca attgataaca caaggtcagg
     1261 tcggtcaact ctttcaactg aagccctgtc aactcttccc atttatcaat cttgtattgg
     1321 agagaattgc ggtgcagata gagttgctgg gctgttttag tgagaacagc actattttcc
     1381 caaagagaga gaatgatttc ctgaatctga tcttgatcca aaatcatctg gtgtagacat
     1441 tccttgattg gcttcaagtc cacgagtctt tctcccatac tccaaagata gagctgagaa
     1501 aaagtatgaa caccttggtg accctgacgc caccatgtct tgaacaaatc ccgctcagct
     1561 ttgattaagt ctgatagggc ttgatgtccc gtctgagacc aaacctgacc caacatgata
     1621 gaaagacgaa gtccaaagtc atactcaacc gcttcaatcg tatcacttaa aatatctctt
     1681 acagaagtgt atttgtcttg ttgaagcacg aaaacataat cctgagctcc gacctgtagc
     1741 actgtctgac aattcggaaa aagagtccgc atcatatcta gccaagaagc cagattttcc
     1801 tgctgaaaat aagaaagatg gcaataaacc aactgaatct ttttaaaaac ttgcggtgcc
     1861 tgtcccttgc cctcaaccag ataggaatac caagggttta gcgaacgagc ctgctcctgc
     1921 tgggtcaaaa gggcaaccaa ctgcttttca cgctcgctga gcccagcttc ctccagcaaa
     1981 atccactgct gagaagctaa agggagcgtg agatagccct ctttctctac tggttggtct
     2041 gaaatccgag cctcaggaaa ccagtcttgt agttcttttg ccctcatgtt ctagccctcc
     2101 actttttgga tgcaccatga aaccaaactc tcaagacgtt ccagattctc agtcatatgg
     2161 agatagccca taaccgcttc aaatcccgtg gacatacgat aagtcacgac atctgcattt
     2221 ttagcctttg tgtggctatt ggtattgcgg ccacgtttgt agatttcttc ttctttttcc
     2281 gttaggacct gttcctccaa catgagagca atcaggcgag cctgagcctt ggctgacacg
     2341 tacttagttg cttcttgatg gagtttattg ggtttggtca tacctttgag gatgaggtga
     2401 cggcgaatat acatagaata caccgcatcc ccctcaaagg ctagcgcaat cccgttaatg
     2461 agattgacat caatcacgtg tccacctcac tccatccttg gtatcaagga gcttaattcc
     2521 ttgagtaacc aattggtcac ggatttggtc tgctgtcgca aagtcacgat tggcacgcgc
     2581 ctcttggcgt ttttgaatca agtcttcaat ctctgcatcc aaaacttcct caacaaagac
     2641 aattccaaaa atttctaaca tatctgcaag agcttgcttg acacttgcat catagttccc
     2701 tgagttgatc catttggcca tttcaaagac aactgtgata ccgttggcag cattaaaatc
     2761 ttcatccata gctgctacaa acttatcttt aaagttttgt aactcttggg catccacatt
     2821 tcctgtaaat ggttgttcgt aagtattctt gagatacttg agattggtct cggcatcgcg
     2881 aactgccttt tccgtgaagt tgataggctt acggtagtgc tgggtcgcaa agaagaaacg
     2941 aagtacttgc ccatcaagag ttttaagggc atcgtgtacc gtaatgaagt tacccaagga
     3001 cttagacatt ttgacattgt cgatattgac aaagccattg tgcatccagt agttagcaaa
     3061 agccttgcct gttttagctt cagactgggc aatttcattg gtgtggtgtg gaaactctag
     3121 gtcagctcca ccaccgtgga tatcaatggt atcacctaaa atctctgtcg acatgactga
     3181 acactcaata tgccaacccg gacgtccagg tccccaagga ctatcccaag aaatctcacc
     3241 tggtttggaa gatttccata gagcaaagtc tacaggattt tccttacgag ccgtttcttc
     3301 atcggtacga cctgaagcac ctagctccaa atcttccaag gttttattag ccaatttagc
     3361 atagttgtgg gatttttcta cacggaaata gacatcccct tgactctcat aggcaaagcc
     3421 tttctcgatc aagtcttcca caaaacggat gatgtctgcc ataaactcca ctacacgcgg
     3481 atggcgagtc gcaggtttca cgcccaatgc cgtcacatcc tcacgaaagg cagcgatgta
     3541 cttatccgca acctcctgag gcgtgatacc ttcttccctg gcacggttga taatcttatc
     3601 atccacatct gtaaaattgg aaatataggc aaccttatac ccacggtact caaaatagcg
     3661 acgaatcgta tcaaaagcta ccgtcgaacg ggcgtttcct acgtggatat agttgtacac
     3721 cgttggccca caaacataca tcttgatctt gccgtcctca atcgggacaa attctcgcaa
     3781 atcacgagac atggtgtcat agattttaat cataaatcat aatcaggaaa gctgaaatcc
     3841 aagaacaatt agtttcatca ctaaaagttc aagtaaattt cagtccgaat atctctacac
     3901 ttcggaatcc cttgctcctt tctcattcag ataaaccacc tgagtctgtt tgacaaagcc
     3961 aattttttca tacaaacgtt tggcacctac attgctatct tccactgcaa tctgaaattc
     4021 cttgtcattt tgctcaatta gttggttgac gagggatttt gctaagtagc ttccatagcc
     4081 ttttccacgt tcaggttcca atattgctaa accgtagagg taattcgtat tagtcgataa
     4141 atcaaccgta caagttccaa taacctgacc agcttttaat aaaatatata gtcggctttc
     4201 tggatctttc agagcttcag cgacatatct atccacaact tctctcgatt catgttcctc
     4261 tgaaaatgcc tgaaatttta attgactaat ttgatcctga tacgaactat ctgctaacaa
     4321 aacttcaaga tgggaaacat ttgctaaggg ataaggtctt ctatccttac ctaaccaagt
     4381 ttctgtctct tcatcctcga ttagtcccca gttactggca aagtcaggat gattctctaa
     4441 aaaaatacgt tctgtctgaa aagtgactga ccgaatgggg aaagaagctg tttctctctc
     4501 aaaactagta aacaatgcac gcgcaatccc ctgacggcga tgacctggat gaaccagtat
     4561 cgtcacttct acatcttggt catctgcata gacagttaat aaaccaacaa gttcgccttt
     4621 ttcataataa aggaaaaagg cgggcatgtt tgggtcaaaa ttaagcatgt tagagagata
     4681 gggatcgcga taggtaccgt catagttttg gcaacagtta attacttttt tcgcctcaga
     4741 tagctcctct tggcttaact tgtttcttgc ttgaatcata taggtatcct ctacaaacca
     4801 gacgatctgt gactggcatc tttagcctgc tcgagtttat tgacataata ctctcgtttt
     4861 tcttcgactt cgtgaatgac aggctcatct ttcttaccat gaagacggac aatcttggcc
     4921 ggaataccga caaccgtcac gtcactaggt acatctgcta cgacaactgc tgcagcaccg
     4981 accttggcat tttcaccaat ttccacaggc ccgataactt gggcatgggc tgatatgagg
     5041 gctccctttc gtacagtcgg atggcgtttg ccacagtctt tccctgttcc cccgagagtc
     5101 actccgtgat agagaagaac gcctttttca acaatcgctg tctctccaat caccagacca
     5161 gaaccatggt caataaaaac acctgaatca atctgggctc ctggatgaat ctcaatctga
     5221 gtccaaaagc gccaaaactg actgtacata cgagctaata gtttgaagcc gtgcttccag
     5281 agaaaatgcg agagacggtg ggccgccaag gccttgacac ctggataagt cagcaaaacc
     5341 tccaaagtgg tgcgggccgc tggatcattt tcttttacaa tatcaatggt ttcgcgccac
     5401 caccccatac atttctcctt ttcttattct gaatcttttg atgtttctgt aaattctttc
     5461 ttaggtttgt aatccttttg atgacgtggg cggtgagggc gctcagactt ttcacctttt
     5521 tcatcatgct caggttttgg cggacgaggt agaagagcct tcatagaggc atcgatacgg
     5581 cctttttcat caatcttgat aaccttaaca tcaacttcat ccccgatttc taccaaatcc
     5641 tctacacgat tggtacgagt ccaagccatc tcagagatat gaacaagggc atctgtctta
     5701 tcaaagaggt taacaaaggc accaaatttc tcgatacgaa cgactttagc acggtaaact
     5761 tcatccactt tggcttcacg aaccaaacca gcaataattt ctttggcacg gttaatagca
     5821 tcttggtcac tagagtagat agacacattt ccttcttcgt ctatatcaat cttaacacct
     5881 gtttcagcga taatcttgtc gatggtttct ccacccttac cgatgacaat cttaatcttg
     5941 tccacatcaa tcttgatcgt atcaattttc ggagcagttg gagccaattc tggacgaact
     6001 tctggaatgg ttgcttcaat gacatcaagg atttcaaaac gcgctttctt ggcttgagca
     6061 agagcctccg tcaagatttc tgcagtaatc ccttgaatct tgatatccat ttgaagggct
     6121 gtaatcccat cacgagtacc tgcaaccttg aagtccatat ctccaaagtg atcttccaaa
     6181 ccttggatat ctgtcaatac tgtgtagtta tttccatctg agataagtcc catagcaata
     6241 ccagctactg gcgccttgat tggcacacca ccagccataa gggcaagagt tcccgcacag
     6301 atagaagctt gagatgaaga accgtttgat tccaaaactt ctgctactag acggatagcg
     6361 tatgggaatt cttccaagct tggcaagact tgagcaagag cacgctcacc aagggcaccg
     6421 tgaccgattt cacgacgacc tggcgcaccg taacgacctg tttcccctac agaatattga
     6481 gggaagttat agtggtgcat aaagcgtttc ttgtactctg gatccaaacc atcaatgatt
     6541 tgagtttctc ccatcggagc caaggtcaag actgaaagag cttgagtttg cccacgagta
     6601 aagagacctg aaccatgtac acgaggaagg aagtcaacaa ccgcatccaa aggacggatt
     6661 tcatcgacct tacgaccatc aggacgcacc ttgtcttctg taattaaacg tcgcacttct
     6721 gcgtgttcca tttgttccaa gatttcagcc acatcacgca taatacggtc aaattcttcg
     6781 tggtccgcat atttttcctc gtaaacagca gtcacttggt ctttcactgc ttgagttgca
     6841 gcttcacggg ccaatttctc ttctacttga actgcctttt ggaggtcact gttgtaggct
     6901 gcaatgattt cagcttgcaa ttcagcatcc acgtgaagca attccacttc tgctttttct
     6961 ttaccgacag cagcaacgat ttcttcttgg aaggcaatca attctttgac agcttcgtgc
     7021 cctttaagaa gcgcttccaa catgatttct tctgacaatt ctttggcacc agactctacc
     7081 atgttgatag cgtgcttggt tccagctact gtcaattcaa gaagagattg ctctgcttgt
     7141 tcttgacttg ggttgatgat gatttggcca tctacatatc ccacttgtac cccagcaatt
     7201 ggtccgtcaa atggaatatc tgaaatagac agtgccaaag atgaaccaaa catagcagcc
     7261 attggtgcag atgcattttc atcataagaa agcactgtat tgatgacttg gacttcatta
     7321 cggaaacctt ccgcaaacat aggacgaatc ggacggtcaa tcaaacgcgc tgtcaaggtc
     7381 gcatctattg aaggacgtcc ttcacgtttc ataaagccac caggaaactt cccagccgca
     7441 tacatttttt cttcgtagtt gacttggagg gggaagaaat ccccagttgc cattttctta
     7501 gacataacgg cagcagtcaa gacagttgac tcaccgtaac gtacgacaac agagccattt
     7561 gcttgcttag caacctgacc agtctctaca attaactcac gacccgcaaa agtcgtttga
     7621 aacacttgtt ttgccatttt aatccccttt ggattgatga aattatacgc cttgcctaca
     7681 aagatcaaga taccaaggac gtcaaaagca aagtaaaaat aggaaactga cgaagtcttc
     7741 gatgaagaca agacagttta tcttttttac acagcttttc ggccgtgttc aattactcaa
     7801 gataccaagc cgtcaagcaa ctcaaaggaa aataggaaat cgaacgacgg agcgactgct
     7861 cctagggaga tttatctttt tccacagagt tgtaggcaag ttcagttaca caggatacta
     7921 agggcgtgaa gaatcccgta tgaaaatagg agattgacgc agtgtaccat gtacaccagg
     7981 aaatctatct ttttcactag ggatttagcc cgtgttcaat tactcaagat accaagccat
     8041 caaacaactc aaaggaaaat aggaaatcga acgacggagc gactgctcct agggagattt
     8101 atctttttcc acagagttgt aggcgtgttc agttttcaag atacatcatt agaaagattt
     8161 aatactaaag tatctaaagc tttcacgcta atcgctatcg ggcgattagc taaatgcttt
     8221 actaactctt tcgtcaaata acatcgattt gactcactcg tgtcgttaaa tcttacagtt
     8281 taaatacatt gtattattta ataccttcat ctttgtatca agtacgtaca gaatctattt
     8341 tatcatattt ttcttaaaaa gtgaggtctt taccattaaa aaggaaccat tcccctcacc
     8401 tgagaagaat ggtttgcttt tattatccta gagactggtg attaaacaag gcatgggttg
     8461 cttgatggat gtattttgct gtatcagcat tattcatcgt atagagatgc acaccggcaa
     8521 catcctgagt taccaagtcc acgatttggt ccactgcata ggcaagtcct gctgctctga
     8581 gcgactcagg gtcatgctca tacttgtcta agatggcttt aaatttgcgt ggaagatgga
     8641 tattctcaca agtcttcaag agtcggagag cctgatttcg attcagaatt ggcataattc
     8701 ctgcatgaat gggaacatca atcccagcca agatacactt gtcctgaaaa tcatagaagc
     8761 gctcatcgtc aaagaagagc tgagttacga ggctcgaaca gcctgcatcc actttcttct
     8821 taagattttg aatatctgaa atctgatttg gcgaatctgg atgcccttct ggatagcaag
     8881 ctccaataat atcaaagtga ggggctcgtt ccttgataag ctcaatcaag tcggttgcat
     8941 agcggaaatc cttttgtggt tccacgtctg gaataatatc cccacgaaga gccaagattt
     9001 tctgcacccc aactttgtcc aagtcagcaa tagtttcagc aaccttgtcc ttagttagat
     9061 aaatagctgg caagtgggca atggtcggaa tcgccaagtc attttggata aagtcagcca
     9121 aacgaaccgt cgtttccttg atattaaatt tattattgct ggcagttaca ctgataaaat
     9181 ggggagccaa ctcctgcata tactgcaagg ctgaaataat gttaccatta cccacggctg
     9241 ggtttggagg gaacacttca aatgagagtg acggtgtttg gcgtgacata tgtaataacc
     9301 ttttctagtt gatttctttt tgaacaacca ctgtatggag agaaatccaa tcttacaatt
     9361 tctcacgcgc agctttagct gcttcaacaa ggcggatcaa gctttctttt gtttctggga
     9421 taccacgtgt tttcaaacca cagtcagggt tgatccaaac tttcttgctt ggcactttag
     9481 caaggatggc ttcgattgtg ttgtcgattt cgccttcatt tggtacacga ggtgagtgga
     9541 tatcgtaaac cccaggtccc acttctgttt ggaagttttt cgctttgagt tcgtccaaga
     9601 tttcaaggtt tgaacggcta gcttcaaagg aaataacgtc tgcatccatg ttatcgatag
     9661 ctgggatgat atctgtaaat tctgagtaac acatgtgagt gtggatttgt gtgtctggcg
     9721 ctactgttga gtgtaccaag cggaaggcag gaatagccca gtcaaggtag tcttcgtacc
     9781 agtcgctacg gcggagtggc aatttttcac gaagagcagc ctcgtcgatt tggatgattt
     9841 tcacaccagc agcttcaagg tcaagtactt catccttgat agcaagggcg atttggagag
     9901 ttgaatcctt gatagagatg tcttcacgtg ggaatgacca gttaaggatg gtaacaggtc
     9961 cagtcaacat acctttaaca ggtttgtttg tacgactttg tgcatagcta gaccatttaa
    10021 cagtgatagg gttaagacga gtgacatcac cccagatgat tggtggtttt accccacgca
    10081 taccgtatga ttgtacccat ccatttttag agaagaggta tcctgacaag ttttgaccga
    10141 agtactcaac catgtcatta cgctcaaatt caccgtgaac aaggacatca aagtcaatat
    10201 cttcttgcca cttgatccat tcgtcaatcg tttcagcaag gaaagcatcg tactcttttt
    10261 gagacaattc acctttacgg taagccaaac gtttggcacg aacttctttt gtttgaggga
    10321 atgaaccaat cgttgttgtt ggaagagctg gaagtttgaa agcttcttct tggatagctt
    10381 cacgttctgc aaagactggc aaacgagtgt agtctgcgtc tgtcaagcca gcgatacgcg
    10441 cacgaagttc agcattttca ccaacacgct cagtcgcaaa gagttctttg ttggctgcaa
    10501 gagcttctga accttgacca ttgcggatag catccaagtc acggatttca tccaattttt
    10561 caactgcaaa ggcaaagtgg ttcaagagtg ctggttcaaa ttcttcatta gcagttgtaa
    10621 atggcacatg aagaagtgag caagagcttg tcaaaacgat gttttcagct gggatttgct
    10681 caagaacagc caagctcttt tcgtagttgt tgcgccagat gtttttacca ttgacaatac
    10741 ctacatagag agtcttgtca gctgggaagc cacctttaac gagttcaaga gttttcttac
    10801 cttcaacaaa gtcaagaccg atagcatcta ctggtaagtt tacaaggtca gcgtatacgt
    10861 cacgaacatc accgaaataa gtttgaagca agacttcaag accttttttg tcagccaaga
    10921 gtttgttgta aaggttcaag aagagagctt tttcttcagc tgtcaagtct tttacaagag
    10981 ccgcttcatc caattggatg cgactcgcac caagttcagc caatttagca aaaacttctt
    11041 ggtaagcagc cactaagcta tctacgaagt cgtctgcttt cacgccttct tcaaagtctg
    11101 acaattgaag gaaagtgaag ggacctacaa gaacaggacg agtgttcaat ccaagttctt
    11161 ttgcttcttg gaactcatcg aaaatcttgt gaccagccaa ttttacttga gtgtcttttt
    11221 caaatttagg aacgatgtag tggtagttag tgttgaacca tttcttcatt ggaagggcgc
    11281 gaacgtcccc tttttctccc tggtaaccac gtcccaaagc gaagtagcgc tcaaggtcag
    11341 acaagtccaa gttttgaacg gatgcaggca ccacgttgaa aaggaaagcc gcatctagga
    11401 agttatcata gtgagaaaag tcatttgatg gaatttcagt gatgcctttt tctttgacaa
    11461 tgttccagtg tttagcacgc aagtcttttg ctgctgctaa aagttcttct tctgagattt
    11521 cttttctaaa gtatttttca gttgtaaatt ttaattcacg gaattcgccc aaacgaggga
    11581 aaccgatgat tgtagttgac atgatgtgtc ctccaaaatt tgttgttgaa actatcttaa
    11641 cagaaaagaa agcgtctgta taattgtaaa aaattagggt ttgatatagt ttgaaactat
    11701 atatctgttt cggacaaaag aaaaagactt gagacacatg cctcaaatcc cttgtataac
    11761 ttgtttttag ctgtatttta gttagaatgc tcaaacgcgt tattagtaat tcttataagt
    11821 gactatggct tgttattaga aaagactata actgattcta gtcaactttt tccctgttca
    11881 agtcggacga ctgctagtat ctttcctaga ctagctagga cttttaagac tgtgtccaac
    11941 tgtggactag tcttacctgt ctccatccta gctataacag gctgactcac tccactgact
    12001 tcctctagct ttttctgact gattccttgc ttatttctgg cttcaatcaa ctcgctcatg
    12061 atagctactc gcatatcact ttcaaggatt tcctccttgc taaagagctc agatagacat
    12121 ccttccaatt actcccaata gcactattct tcatcactta cccctctttc ttttagatct
    12181 atgtattttt tgaaaaaatg agcgaattat gattcgatag attgaccagc aggtttaaag
    12241 ttggtgctag cctgtttctt aagcgctttt ccttttctag ggtaaagcag atcctgcttg
    12301 cttaacccca attttccacg atgaatccaa tagtaaatgg ttgaaattcc cacgttaacc
    12361 cctttagcca tcaccatcat ttcaggcaaa aatttttggt tatgatagtg gagaatcttt
    12421 tcctttagtt ccttggtcaa gcttgatttc ttgaccgagc gcttgcgatt gttttcataa
    12481 gactgttgag catagtcggc agaataaacc tctttgaagc gcccttttcc aagacattgt
    12541 cggactgtcc cacgcttgat ttcagtgtgg atagtttgag gagcttttcc aagtagagag
    12601 gcaatttctc tatttgattt cccttctttt ttccatcttt cgattaagcg acggctatcg
    12661 attgtcaaat gtttggcttt tgtagt
//
LOCUS       DAVLVP010000051        12294 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.051, whole genome shotgun sequence.
ACCESSION   DAVLVP010000051 DAVLVP010000000
VERSION     DAVLVP010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12294
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.051"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..212
                     /locus_tag="VYN69_001354"
     CDS             <1..212
                     /locus_tag="VYN69_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SP_0924 family bacteriocin-like peptide"
                     /protein_id="HEV1533617.1"
                     /translation="TKNINLTNEELELIQGGADPYGKNPNGRYDWEIEPVLTLPVHGF
                     CPRGTYDLGYIGGGNHLCKGSAARF"
     gene            266..937
                     /locus_tag="VYN69_001355"
     CDS             266..937
                     /locus_tag="VYN69_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1533618.1"
                     /translation="MKKRAIQILLALSLIFYKSTWFWRVFNHLAKPYLPASREFFQIL
                     LLLESGVLLSAVIYLLVFAGKKIFHFKWQLRYFIYLLLGYIISYMSDFLFSYFISPPS
                     NQISLNETIEMMGRQEFPYFLLIVCFIAPIAEELIYRGVLMTTFFKNSPWYGDVLLSA
                     IIFGYIHINFALTPLAFFIYASGGLILALLYRMTKNLYYPILVHILINITAFWDVWLL
                     LFSGS"
     gene            complement(989..1177)
                     /locus_tag="VYN69_001356"
     CDS             complement(989..1177)
                     /locus_tag="VYN69_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1533619.1"
                     /translation="MFIFYPQLLYLFIIPYFRSFPKRKKEHPILLHYLPFSRQSYFQN
                     QFKKISGYTPLQYRKEKR"
     gene            1170..2078
                     /locus_tag="VYN69_001357"
     CDS             1170..2078
                     /locus_tag="VYN69_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEV1533620.1"
                     /translation="MNIQQLRYVVAIANSGTFREAAEKMYVSQPSLSISVRDLEKELG
                     FKIFRRTSSGTFLTRRGMEFYEKAQELVKGFDIFQNQYANPEEEKDEFSVASQHYDFL
                     PPTITAFSERYPDYKNFRIFESTTVQILDEVAQGHSEIGIIYLNNQNKKGIMQRVEKL
                     GLEVIELIPFHTHIYLCEGHPLAQKEELVMEDLADLPTVRFTQEKDEYLYYSENFVDT
                     SASSQMFNVTDRATLNGILERTDAYATGSGFLDSDSVNGITVIRLKDNLDNRMVYVKR
                     EEVELSQAGTLFVEVMQEYFDQKRKS"
     gene            2075..2536
                     /locus_tag="VYN69_001358"
     CDS             2075..2536
                     /locus_tag="VYN69_001358"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEV1533621.1"
                     /translation="MKKRAIVAVIVLLLIGLDQLVKSYIVQQIPLGEVRSWIPNFVSL
                     TYLQNRGAAFSILQDQQLLFAVITLVVVIGAIWYLHKHMEDSFWMVLGLTLIIAGGLG
                     NFIDRVSQGFVVDMFHLDFINFAIFNVADSYLTVGVIILLIAMLKEEINGN"
     gene            2526..3413
                     /locus_tag="VYN69_001359"
     CDS             2526..3413
                     /locus_tag="VYN69_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1533622.1"
                     /translation="MEIKIETGGLRLDKALSDLSELSRSLANEQIKSGQVLVNGQVKK
                     AKYTVQEGDVVTYHVPEPEVLEYVAEDLPLEIVYQDEDVAVVNKPQGMVVHPSAGHTS
                     GTLVNALMYHIKDLSGINGVLRPGIVHRIDKGTSGLLMIAKNDDAHLVLAQELKDKKS
                     LRKYWAIVHGNLPNDRGVIEAPIGRSEKDRKKQAVTAKGKPAVTRFHVLERFGDYSLV
                     ELQLETGRTHQIRVHMAYIGHSVAGDEVYGPRKTLKGHGQFLHAKTLGFTHPRTGKTL
                     EFKADIPEIFKETLERLRK"
     gene            3416..5311
                     /gene="cbpE"
                     /locus_tag="VYN69_001360"
                     /gene_synonym="pce"
     CDS             3416..5311
                     /gene="cbpE"
                     /locus_tag="VYN69_001360"
                     /gene_synonym="pce"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine esterase CbpE"
                     /protein_id="HEV1533623.1"
                     /translation="MKKKLTSLALVGAFLGLSWYGNVQAQESSGNKIHFINVQEGGSD
                     AIILESNGHFAMVDTGEDYDFPDGSDSRYPWREGIETSYKHVLTDRVFRRLKELSVQK
                     LDFILVTHTHSDHIGNVDELLSTYPVDRVYLKKYSDSRITNSERLWDNLYGYDKVLQT
                     AAEKGVSVIQNITQGDAHFQFGDMDIELYNYENETDSSGELKKIWDDNSNSLISVVKV
                     NGKKIYLGGDLDNVHGAEDKYGPLIGKVDLMKFNHHLDTKNSNTKNFIKNLSPEIIVQ
                     TSSSPIGSYDSGQEINREYISWLESMGIQHINAASKDYDATVFDIRQDGLVNISTSYK
                     PIPSFQAGWHKSSYGNWWYQAPDSTGEYAVGWNEIKGEWYYFNQTGILLQNQWKKWNN
                     RWFYLTDSGVAAKNWKKIAGIWYYFNKENQMEIGWIQDKEQWYYLDVDGSMKTGWLQY
                     KGQWYYFAPSGEMKTGWVKDKEAWYYMDSTGIMKTGEIEVAGHHYYLEESGAMKQGWL
                     KKANDWYFYKTDGSRAIGWIKDKDKWYFLKENGQLLVNGKTPEGYTVDSSGAWLVDVP
                     IEKSATIKTTSHSEIKESKEVVKKDLENKETSQHESVTNSSTSQDLTSSTSQSSETSV
                     NKSESEQ"
     gene            5391..6521
                     /locus_tag="VYN69_001361"
     CDS             5391..6521
                     /locus_tag="VYN69_001361"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004240022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEV1533624.1"
                     /translation="MNNRSETMKYKRIVFKVGTSSLTNEDGSLSRSKVKDITQQLAML
                     HEAGHELILVSSGAIAAGFGALGFKKRPTKIADKQASAAVGQGLLLEEYTTNLLLRQI
                     VSAQILLTQDDFVDKRRYKNAHQALSVLLNRGAIPIINENDSVVIDELKVGDNDTLSA
                     QVAAMVQADLLVFLTDVDGLYTGNPNSDPRAKRLERIETINREIIDMAGGAGSSNGTG
                     GMLTKIKAATIATESGVPVYICSSLKSDSMIEAAEETEDGSYFVAQEKGLRTQKQWLA
                     FYAQSQGSIWVDKGAAEALSQHGKSLLLSGIVEAEGAFSYGDIVTVFDKESGKSLGKG
                     RVQFGASALEDMLRSQKAKGVLIYRDDWISITPEIQLLFTEF"
     gene            6531..7793
                     /locus_tag="VYN69_001362"
     CDS             6531..7793
                     /locus_tag="VYN69_001362"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEV1533625.1"
                     /translation="MVSRQEQFEQVQAVKKSINTASEEVKNQALLAMADHLVAATEEI
                     LAANALDMAAAKGKISDVMLDRLYLDADRIEAMARGIREVVALPDPIGEVLETSQLEN
                     GLVITKKRVAMGVIGIIYESRPNVTSDAAALTLKSGNAVVLRSGKDAYQTTHAIVTAL
                     KKGLETTTIHPNVIQLVEDTSRESSYAMMKAKGYLDLLIPRGGAGLINAVVENAIVPV
                     IETGTGIVHVYVDKDADEDKALSIINNAKTSRPSVCNAMEVLLVHENKAASILPRLDQ
                     MLVAERKEAGLEPIQFRLDSKASQFVSGQAAETQDFDTEFLDYVLAVKVVSSLEEAVA
                     HIESHSTHHSDAIVTENAEAAAYFTDQVDSAAVYVNASTRFTDGGQFGLGCEMGISTQ
                     KLHARGPMGLKELTSYKYVVAGDGQIRE"
     gene            7797..8594
                     /gene="proC"
                     /locus_tag="VYN69_001363"
     CDS             7797..8594
                     /gene="proC"
                     /locus_tag="VYN69_001363"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEV1533626.1"
                     /translation="MKIGFIGLGNMGASLAKSVLQTRTSDEILLANRSQAKVDAFIAD
                     FGGQASSNEEMFAEADVIFLGVKPAQFSELLSQYQTILEKRESLLLISMAAGLTLEKL
                     ASLIPSQHRIIRMMPNTPASIGQGVISYALSPNCRAEDSELFCQLLSKAGLLVELGES
                     LINAATGLAGCGPAFVYLFIEALADAGVQTGLPRETALKMAAQTVVGAGQLVLESQQH
                     PGVLKDQVCSPGGSTIAGVASLEAHAFRGIVMEAVHQAYKRTQELGK"
     gene            8814..9452
                     /locus_tag="VYN69_001364"
     CDS             8814..9452
                     /locus_tag="VYN69_001364"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEV1533627.1"
                     /translation="MSKGFLVSLEGPEGAGKTSVLEALLPILEEKGVEVLTTREPGGV
                     LIGEKIREVILDPSHTQMDAKTELLLYIASRRQHLVEKVLPALEAGKLVIMDRFIDSS
                     VAYQGFGRGLDIEAIDWLNQFATDGLKPDLTLYFDIEVEEGLARIAANSDREVNRLDL
                     EGLDLHKKVRQGYLSLLDKEGNRIVKIDASLPLEQVVETTKAVLFDGMGLAK"
     gene            9449..10339
                     /locus_tag="VYN69_001365"
     CDS             9449..10339
                     /locus_tag="VYN69_001365"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000806705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEV1533628.1"
                     /translation="MKQDQLKAWQPAQFDRFVRILEQDQLNHAYLFSGFFGSLEIAQF
                     LAKSLFCTDKVGVLPCEKCRSCKLIEQGEFPDVTLIKPVNQVIKTERIRELVGQFSQA
                     GIESQQQVFIIEQADKMHPNAANSLLKVIEEPQSEVYIFFLTSDEEKMLPTIRSRTQI
                     FHFKKQEEKLILLLEQMGLVKKKATLLAKFSQSRAEAEKLANQASFWTLVDESERLLT
                     WLVAKKKESYLQVAKLANLADDKEKQDQVLRILEVLCGQDLLQVRVRVILQDLLEARK
                     MWQANVSFQNAMEYLVLKEI"
     gene            10379..10696
                     /gene="yabA"
                     /locus_tag="VYN69_001366"
     CDS             10379..10696
                     /gene="yabA"
                     /locus_tag="VYN69_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEV1533629.1"
                     /translation="MDKKELFDALDDFSQQLLVTLADVEAIKKNLKSLVEENTALRLE
                     NSKLRERLGEVEADAPVKAKHVRESVRRIYRDGFHVCNDFYGQRREQDEECMFCDELL
                     YRE"
     gene            10699..11568
                     /gene="rsmI"
                     /locus_tag="VYN69_001367"
     CDS             10699..11568
                     /gene="rsmI"
                     /locus_tag="VYN69_001367"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001166894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEV1533630.1"
                     /translation="MQIQKSFKGQSPYGKLYLVATPIGNLDDMTFRAIQTLKEVDWIA
                     AEDTRNTGLLLKHFDISTKQISFHEHNAKEKIPDLIGFLKAGQSIAQVSDAGLPSISD
                     PGHDLVKAAIEEEIAVVTVPGASAGISALIASGLAPQPHIFYGFLPRKSGQQKQFFGS
                     KKDYPETQIFYESPHRVADTLENMLEVYGDRSVILVRELTKIYEEYQRGTISELLESI
                     SETSLKGECLLIVEGASQDVEEKDEEDLFLEIQARIQQGMKKNQAIKEVAKIYQWNKS
                     QLYAAYHDWEEKQ"
     gene            11795..>12294
                     /locus_tag="VYN69_001368"
     CDS             11795..>12294
                     /locus_tag="VYN69_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEV1533631.1"
                     /translation="MRTYENKEELKAEIEKTFEKYILEFDNIPENLKDKRADEVDRTP
                     AENLAYQVGWTNLVLKWEEDERKGLQVKTPSDKFKWNQLGELYQWFTDTYAHLSLQEL
                     KAKLNENINSISAMIDSLSEEELFEPHMRKWADEATKTATWEVYKFIHVNTVAPFGTF
                     RTKIRK"
BASE COUNT         3699 a         2110 c         2900 g         3585 t
ORIGIN      
        1 tgacaaaaaa tataaatcta acaaatgaag aattagagct gatacaaggt ggagcagatc
       61 catatggtaa aaatcctaat ggtaggtacg attgggaaat agaaccagta ttaactctgc
      121 cggttcatgg attttgtccc agaggcacct atgatttagg atatattgga ggaggcaatc
      181 atctttgcaa aggaagtgct gcgagatttt aagtaaaatt tattagtaat atgaagaaac
      241 aaggggagaa agcagagaat ttaatatgaa aaaacgggct attcaaattt tactagcatt
      301 gtccttaatt ttttacaaat caacttggtt ttggagagtt ttcaatcacc tcgcaaagcc
      361 ctatctacca gcaagtcgtg aattttttca gattctgctt ttgctggaaa gtggtgttct
      421 tttgtcagca gtcatctatc tactagtttt tgcaggaaag aagatttttc atttcaagtg
      481 gcagttgagg tactttatct accttttact gggctacatc atttcgtata tgtctgactt
      541 tctcttttcg tatttcatat ccccgccttc aaatcaaatt tctttgaatg aaacgataga
      601 aatgatggga cgacaggagt tcccttattt cttgctcatc gtttgcttca tcgcccctat
      661 tgctgaggaa ttgatttatc gcggtgtgct tatgacaacc tttttcaaaa actcgccttg
      721 gtacggagat gttttgcttt ctgctattat tttcggttat attcatatca attttgcttt
      781 aactcctctt gcttttttca tttatgctag tggaggtctt attttagctc tattgtatcg
      841 catgactaaa aatctctact atccaatact agttcatatt ctcatcaata tcactgcctt
      901 ctgggatgtt tggttactcc tattttcagg aagttagctt actaaaaaaa tgtcggaatt
      961 ttccggcatt ttcttttttc acaaatagtc aacgtttttc tttccgatac tgaagtggtg
     1021 tgtagccact tatttttttg aattgatttt gaaaataaga ttggcgtgag aaaggcagat
     1081 agtgaagaag aataggatgt tcttttttcc tttttggaaa acttctaaaa tatggtataa
     1141 tgaaaagata aagaagttgg gggtagaaga tgaacattca acaattacgc tatgttgtgg
     1201 ctattgccaa tagtggtact tttcgtgaag ctgctgaaaa gatgtatgtt agtcagccga
     1261 gtctgtctat ttctgttcgt gatttggaaa aagagttggg ctttaagatt ttccgtcgga
     1321 ccagctcagg gactttcttg acccgtcgtg ggatggaatt ttatgaaaaa gcgcaagaat
     1381 tggttaaagg atttgatatt tttcaaaatc agtatgccaa tcctgaagaa gaaaaagatg
     1441 aattttccgt tgctagccag cactatgact tcttaccacc aactattacg gccttttcag
     1501 agcgctatcc tgactataag aacttccgta tttttgaatc aactactgtt caaatattag
     1561 atgaagtggc gcaagggcat agtgagattg ggattatcta cctcaacaat caaaataaaa
     1621 aggggattat gcaacgggtt gaaaagttag gtctggaggt catcgaattg attcctttcc
     1681 atacccatat ttatctctgt gagggtcatc ctttagccca gaaagaggaa ttagtcatgg
     1741 aggatttagc ggatttacca acggttcgtt tcactcaaga gaaagacgag tacctttatt
     1801 attcagagaa ctttgtcgat accagcgcta gctcacagat gtttaatgtg acagaccgtg
     1861 ccaccttgaa tggtattttg gagcggacgg acgcctatgc gacaggttct ggatttttag
     1921 atagtgacag tgttaatggc attacagtta ttcgtctcaa ggataaccta gataaccgca
     1981 tggtctatgt taaacgtgaa gaagtggagc ttagtcaagc tgggactctc ttcgtagaag
     2041 tcatgcaaga atattttgat caaaagagga aatcatgaaa aaaagagcaa tagtggcagt
     2101 cattgtactg cttttgattg ggctggatca gttggtcaaa tcctatatcg tccagcagat
     2161 tccactgggt gaagtgcgct cctggattcc caatttcgtt agcttgacct acctgcaaaa
     2221 tcgaggtgca gccttttcta tcttacaaga tcagcagctg ttattcgctg tcattactct
     2281 ggttgtcgtg ataggtgcca tttggtattt acataaacac atggaggact cattctggat
     2341 ggtcttgggt ttgactctaa taatcgcggg tggtcttgga aactttattg acagggtcag
     2401 tcagggcttt gttgtggata tgttccacct tgactttatc aactttgcaa ttttcaatgt
     2461 ggcagatagc tatctgacgg ttggagtgat tattttattg attgcaatgc taaaagagga
     2521 aataaatgga aattaaaatt gaaactggtg gtctgcgttt ggataaggct ttgtcagatt
     2581 tgtcagaatt atcacgtagt ctcgcgaatg aacaaattaa atcaggccag gtcttggtca
     2641 atggtcaagt caagaaagct aaatacacag tccaagaggg tgatgtcgtc acttaccatg
     2701 tgccagaacc agaggtatta gagtatgtgg ctgaggatct tccgctagaa atagtctacc
     2761 aagatgagga tgtggctgtc gttaacaaac ctcagggaat ggttgtgcac ccgagtgctg
     2821 gtcataccag tggaacccta gtaaatgccc tcatgtatca tattaaggac ttgtcgggta
     2881 tcaatggggt tctgcgtcca gggattgttc accgtattga taagggtacg tcaggtcttc
     2941 tcatgattgc taaaaacgat gatgcgcatc tagtacttgc ccaagaactc aaagataaaa
     3001 agtctctccg caaatattgg gcgattgttc atggaaatct gcctaatgat cgtggtgtaa
     3061 ttgaagcgcc gattggccgg agtgaaaaag accgtaagaa acaggctgta actgctaaag
     3121 ggaagcctgc agtgacgcgt tttcacgtct tggaacgctt tggcgattat agcttagtag
     3181 agttgcaact ggagacaggg cgcactcatc aaatccgtgt ccacatggct tatatcggcc
     3241 attcagtcgc tggtgatgag gtctatggtc ctcgcaagac tttgaaagga catggacaat
     3301 ttcttcatgc caagacttta ggttttactc atccgagaac aggtaagacc ttggaattta
     3361 aagcagatat cccagagatt tttaaggaaa ccttggagag attgagaaag taagaatgaa
     3421 aaagaaatta actagtttag cacttgtagg cgctttttta ggtttgtcat ggtatgggaa
     3481 tgttcaggct caagaaagtt caggaaataa aatccacttt atcaatgttc aagaaggtgg
     3541 cagtgatgcg attattcttg aaagcaatgg acattttgcc atggtggata caggagaaga
     3601 ttatgatttc ccagatggaa gtgattctcg ttatccatgg agagaaggaa ttgaaacgtc
     3661 ttataagcat gttctaacag accgtgtctt tcgtcgtttg aaggaattga gtgtccaaaa
     3721 acttgatttt attttggtga cccataccca cagtgatcat attggaaatg ttgatgaatt
     3781 actgtctacc tatccagttg accgagtcta tcttaagaaa tatagtgata gtcgtattac
     3841 taattctgaa cgtctatggg ataatctgta tggctatgat aaggttttac agactgctgc
     3901 agaaaaaggt gtttcagtta ttcaaaatat cacacaaggg gatgctcatt ttcagtttgg
     3961 ggacatggat attgagctct ataactatga aaatgaaacc gattcatcgg gtgaattaaa
     4021 gaaaatttgg gatgataatt ccaattcctt gattagtgtg gtaaaagtca atggcaagaa
     4081 aatttacctt gggggcgact tagataatgt tcatggagca gaagacaagt atggtcctct
     4141 cattggaaaa gttgatttga tgaagtttaa tcatcattta gatacaaaaa attctaatac
     4201 aaaaaatttt ataaaaaatt tgtctcctga gataattgtt caaacttcct cctcaccaat
     4261 tggttcgtat gatagcggtc aagaaataaa tagggaatat attagttggc ttgaatcaat
     4321 gggaattcaa catataaatg cagccagcaa agactatgat gcaacagttt ttgatattcg
     4381 acaagacggt ttagtaaaca tttcaacttc ctacaagccg attccaagtt ttcaagctgg
     4441 ttggcataag agttcatatg gaaactggtg gtatcaagcg cctgattcta caggagaata
     4501 tgctgtcggt tggaatgaaa ttaagggtga atggtattac tttaaccaaa cgggtatctt
     4561 attacagaat caatggaaaa aatggaacaa tcgttggttc tatttgacag actctggtgt
     4621 tgctgctaaa aattggaaga aaatcgctgg aatctggtat tattttaaca aagaaaacca
     4681 gatggaaatt ggttggattc aagataaaga gcagtggtat tatttggatg ttgatggctc
     4741 tatgaagaca ggttggcttc aatataaggg gcaatggtat tactttgctc catcagggga
     4801 aatgaaaacg ggctgggtaa aagataaaga agcctggtac tatatggatt ctactggtat
     4861 catgaagaca ggtgagatag aagttgctgg acatcattac tatctagaag aatcaggagc
     4921 tatgaagcaa ggctggctta aaaaggcaaa tgattggtat ttctacaaga cagatggttc
     4981 acgagctata ggttggatta aagacaagga taaatggtac ttcttgaaag aaaatggtca
     5041 attacttgtg aatggtaaga caccagaagg ctatactgtg gattcaagtg gtgcctggtt
     5101 agtggatgtt ccgatcgaga aatctgctac aattaaaact acaagtcatt cagaaataaa
     5161 agaatccaaa gaagtagtga aaaaggatct tgaaaataaa gaaacgagtc aacatgaaag
     5221 tgttacaaat tcttcaacta gtcaagattt gacatcctca acttcacaaa gctctgaaac
     5281 gagtgtaaac aaatcggaat cagaacagta gtagaaaaga aggttttagg gccttctttt
     5341 tcctatcaac tcttttctat ttcctgttat tcatgttata atggataaat atgaataatc
     5401 ggagtgagac tatgaaatac aaacggattg tctttaaggt gggtacttct tctctgacaa
     5461 atgaggatgg aagtttatca cgtagtaagg taaaggatat tacccagcag ttggctatgc
     5521 tgcacgaggc tggtcatgag ttgattttgg tgtcttcagg tgccattgcg gctggttttg
     5581 gagccttagg atttaaaaag cgtccgacta aaattgctga taaacaggct tcagcagcgg
     5641 tagggcaggg acttttgttg gaagaatata cgaccaatct tctcttgcgt caaatcgttt
     5701 ctgcacaaat cttgctgacc caagatgact ttgtggataa gcgtcgttat aaaaatgccc
     5761 atcaggcttt gtcggttttg ctcaaccgtg gggcaattcc tatcatcaat gagaatgata
     5821 gtgtcgttat tgatgagctc aaggttgggg acaatgacac tctaagtgct caagtagcgg
     5881 cgatggtcca agcagacctt ttagttttct tgacagatgt ggacggtctc tatactggaa
     5941 atcctaattc agatccaaga gccaaacgct tggagagaat cgagaccatc aatcgtgaga
     6001 ttattgatat ggctggtgga gcaggttcgt caaacggaac tgggggtatg ttaaccaaaa
     6061 tcaaggctgc aactatcgcg acggaatcag gagttcctgt ttatatctgc tcatccttga
     6121 aatcagattc catgattgag gcggcagagg agaccgagga tggttcttac tttgttgctc
     6181 aagagaaggg gcttcgtacc cagaaacaat ggcttgcctt ctatgctcag agtcaaggtt
     6241 ctatttgggt tgataaaggg gctgcggaag ctctctctca acatggaaag agtcttctct
     6301 tatctggtat cgttgaagca gaaggagcct tttcttacgg tgatatcgtg acagtatttg
     6361 acaaggaaag tggaaaatca cttggaaaag gacgcgtgca atttggagca tctgctttgg
     6421 aggatatgtt gcgttctcaa aaagccaagg gtgtcttgat ttaccgtgac gactggattt
     6481 ccattactcc tgaaatccaa ctacttttta cagaatttta gaggtaaact atggtgagta
     6541 gacaagaaca atttgaacag gtacaggctg ttaaaaaatc gattaacaca gctagtgaag
     6601 aagtgaaaaa ccaagccttg ctagccatgg ctgatcactt agtggctgct actgaggaaa
     6661 ttttagcggc taatgccctc gatatggcag cggctaaggg gaaaatctca gatgtgatgt
     6721 tggatcgtct ttatttggat gcagatcgta tagaagcgat ggcaagagga attcgtgaag
     6781 tggttgcctt accagatcca atcggtgaag ttttagaaac aagtcagctt gaaaatggtt
     6841 tggttatcac aaaaaaacgt gtagctatgg gtgtcatcgg tattatctat gaaagccgtc
     6901 caaatgtgac gtctgatgcg gctgctttga ctcttaagag tggaaatgcg gttgttcttc
     6961 gtagtggtaa ggatgcctat caaacaaccc atgccattgt cacagccttg aagaagggct
     7021 tggagacgac tactattcat ccaaatgtga ttcaactggt ggaggatact agccgtgaaa
     7081 gtagttatgc tatgatgaag gccaagggct atctagacct tctcattcct cgtggaggag
     7141 ctggcttgat caatgcagtg gttgagaatg cgattgtacc tgttatcgag acagggactg
     7201 ggattgtcca tgtctatgtg gataaggatg cagacgaaga caaggcgctg tctatcatca
     7261 acaatgctaa aaccagtcgt ccttctgttt gtaatgccat ggaggttctg ctggttcatg
     7321 aaaacaaggc agcaagcatc cttcctcgct tggatcaaat gctggttgca gagcgtaagg
     7381 aagctggact ggaaccaatt caattccgct tggatagcaa agcaagccag tttgtttcag
     7441 gtcaagcagc tgagacccaa gactttgaca ccgagttttt agactatgtc cttgctgtta
     7501 aggttgtgag cagtttagaa gaagcggttg cgcacattga atcccacagc acccatcatt
     7561 cggatgctat tgtgacggaa aatgctgaag ctgcagcata ctttacagat caagtggact
     7621 ctgcagcggt gtatgttaat gcctcaactc gtttcacaga tggaggacaa tttggtcttg
     7681 gttgtgaaat ggggatttct actcagaaat tgcacgcgcg tggtccaatg ggcttgaaag
     7741 agttgaccag ctacaagtat gtggttgctg gtgatgggca gataagggag taagagatga
     7801 agattggatt tatcggtttg gggaatatgg gtgctagctt ggcaaaatct gtcttgcaga
     7861 ctaggacgtc agatgagatt ctccttgcca atcgtagtca agctaaggta gatgctttca
     7921 ttgcagactt tggtggtcag gcttccagca atgaagaaat gtttgcagaa gcagatgtga
     7981 tttttctagg agttaagcct gctcagtttt ctgaactgct ttctcaatac cagaccatcc
     8041 ttgaaaaaag agaaagtctt cttttgattt cgatggcagc tggattgacc ttagaaaaac
     8101 tagcaagtct tatcccaagt caacaccgaa ttattcgtat gatgcctaat acccctgctt
     8161 ctatcgggca aggagtgatt agttatgcct tgtctcctaa ttgcagggct gaggacagtg
     8221 agctcttttg tcagctttta tccaaggctg gtctcttggt tgaattagga gaaagcttaa
     8281 tcaatgcagc gacaggtctt gcaggctgtg gaccagcctt tgtctatctc tttattgagg
     8341 ctttggcaga tgcaggtgtt cagacgggat taccacgaga aacagccttg aaaatggcag
     8401 cccaaactgt ggtaggagct gggcaattgg tcctagaaag ccagcaacat cctggagttt
     8461 tgaaagatca agtttgtagt ccaggcggtt cgactattgc tggcgtagca agcctagaag
     8521 cgcatgcttt tcgaggcata gtcatggagg cagttcatca agcctataaa cgaacacaag
     8581 aactaggtaa ataagaggta gttttatctg cctcttttat ggtggttgat actcttcgaa
     8641 aatcaaattc aaactgcgtc agcttcacct tgccgtactc aagtacagcc tgcggctagc
     8701 ttcctagttt gctctttgat tttcattgag tatgaaatga gaagacaaaa agattgtcac
     8761 aaacccctat ttttttgata gaatagaagt agtaaaaaag aaatgagtta gacatgtcaa
     8821 aaggattttt agtctctctt gagggaccag agggagcagg caagaccagt gttttagagg
     8881 ctctgctacc aattttagag gaaaaaggag tagaggtgtt gacgacccgt gaacctggcg
     8941 gagtcttgat tggggagaag attcgggaag tgattttgga tccaagtcat actcagatgg
     9001 atgctaaaac agagctactt ctctatattg ccagtcgcag acagcatttg gtggaaaaag
     9061 ttcttccagc ccttgaagct ggcaagttgg tcatcatgga tcgttttatc gatagttctg
     9121 ttgcctatca gggatttggt cgtggcttag atattgaagc cattgactgg ctcaatcagt
     9181 ttgcgacaga tggcctcaaa cccgatttga cactctattt tgacatcgag gtggaagaag
     9241 ggctggctcg tattgctgct aatagtgacc gcgaggttaa tcgtttggat ttggaagggt
     9301 tggacttgca taaaaaagtt cgtcaaggct acctttctct tctggataaa gagggaaatc
     9361 gcattgtcaa gattgatgct agtctccctt tggagcaagt tgtggaaact accaaggctg
     9421 tcttgtttga cggaatgggc ttggccaaat gaaacaagat caactaaagg cttggcaacc
     9481 agctcagttt gaccgttttg tccgtatctt agaacaagac cagctcaatc acgcctatct
     9541 cttttcaggt ttctttggaa gcttggaaat agcgcaattt ttagctaaga gcctcttttg
     9601 tacggataaa gttggcgtct taccatgtga gaaatgccga agttgtaagc tgattgaaca
     9661 gggagaattt cccgatgtca ccttgattaa gccagtcaat caggtcatca agacagaacg
     9721 cattagggaa ttggtgggac agttttctca agcagggatt gaaagccagc aacaggtctt
     9781 tattatcgag caagcggata aaatgcatcc caacgcagcc aattctctgc tcaaggtcat
     9841 cgaagaaccc cagagtgaag tttatatttt cttcttgact agcgatgagg aaaagatgtt
     9901 accgacaatc cgaagtcgga cccagatctt ccactttaaa aagcaagaag aaaaacttat
     9961 cttactctta gaacaaatgg gacttgttaa gaaaaaagcg actcttttag ctaagtttag
    10021 tcaatcgcga gctgaagcag aaaagttggc taatcaggca agtttttgga ccttggtcga
    10081 tgaaagtgaa cgcctgctga cttggttagt agctaagaaa aaagaaagtt atctacaggt
    10141 tgccaaatta gccaacttgg cagatgataa ggaaaaacag gatcaggttt tacggattct
    10201 tgaagttctc tgtgggcagg acctcttgca ggtaagagta agagtgattc tacaagattt
    10261 actagaagct agaaaaatgt ggcaagctaa tgtcagcttt caaaatgcca tggaatatct
    10321 ggtcttgaaa gaaatataaa ctcaaaaatg aatgataaag aaaggaaagg gctgttttat
    10381 ggacaaaaaa gaattatttg acgcgctgga tgatttttcc caacaattat tggtaacctt
    10441 agccgatgtg gaagccatca agaaaaatct caagagcctg gtagaggaaa atacagctct
    10501 tcgcttggaa aatagtaagt tgcgagaacg cttgggtgag gtggaagcag atgctcctgt
    10561 caaggccaag catgttcgtg aaagtgtccg tcgcatttac cgtgatggat ttcacgtatg
    10621 taatgatttt tatggacaac gtcgagagca ggacgaagaa tgtatgttct gtgatgagtt
    10681 gttatacagg gagtaggcat gcaaattcaa aaaagtttta aggggcagtc tccctatggc
    10741 aagctgtatc tagtggcaac gccgattggc aatctagatg atatgacttt tcgtgctatc
    10801 cagaccttga aagaagtgga ctggattgct gctgaggata cgcgcaatac aggtcttttg
    10861 ctcaagcatt ttgacatttc caccaagcag atcagttttc atgagcacaa tgccaaggaa
    10921 aaaattcctg atttgattgg tttcttgaaa gcagggcaaa gtattgctca ggtctctgat
    10981 gccggtttgc ctagcatttc agaccctggt catgatttgg ttaaggcagc tattgaggaa
    11041 gaaattgcag ttgttacagt tccaggtgcc tctgcaggaa tttctgcctt gattgccagt
    11101 ggtttagcgc cacagccaca tatcttttac ggttttttac cgagaaaatc aggtcaacag
    11161 aagcaatttt ttggctctaa aaaagattat cctgaaacac agatttttta tgaatcacct
    11221 catcgtgtag cagacacgtt ggaaaatatg ttagaagtct acggtgaccg ctccgttatc
    11281 ttggtcaggg aattgaccaa aatctatgaa gaataccaaa gaggtacaat ttctgaattg
    11341 ctggaaagca tctctgaaac gtctctcaag ggtgaatgtc ttctgattgt tgaaggtgcc
    11401 agtcaggatg tggaagaaaa agacgaggaa gacttgttct tagaaatcca agctcgtatc
    11461 cagcaaggta tgaagaaaaa tcaagctatt aaggaagtag ctaagattta ccagtggaat
    11521 aagagtcaac tctacgctgc ctaccacgac tgggaagaaa aacaataaag ggagatagga
    11581 tgtaataatt ctgtctgttt ctgtttaact taattagtga tgataatata aagatgtatc
    11641 acttggtata gaagctttgg tattaagttt tttattaagc ccatacggaa taccgatggt
    11701 tggagcagca gttatagcgt tcttagaagg tataaataga aaaataaggt cattttaaat
    11761 caaaggattg ataaatcaga aagaaggtga ttttttgcga acatacgaaa ataaagaaga
    11821 actaaaagct gagatagaga aaacatttga gaaatatatt ttagaatttg ataatattcc
    11881 agaaaattta aaagataaga gagctgatga agttgacaga actccagcag aaaaccttgc
    11941 ttatcaggtt ggttggacca acttggttct taaatgggaa gaagatgaaa gaaaggggct
    12001 tcaagtaaaa acaccatcgg ataaatttaa atggaatcaa cttggtgaat tatatcagtg
    12061 gttcacagat acctacgctc atttatctct gcaagagttg aaagcaaaat taaatgaaaa
    12121 tattaattct atctctgcaa tgattgattc gttgagtgag gaagaattat ttgaaccgca
    12181 tatgagaaag tgggctgatg aagcgactaa aacagcgact tgggaagtgt ataagtttat
    12241 tcatgtaaat acggttgcac cttttggaac tttcagaact aaaatcagaa aatg
//
LOCUS       DAVLVP010000052        12100 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.052, whole genome shotgun sequence.
ACCESSION   DAVLVP010000052 DAVLVP010000000
VERSION     DAVLVP010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 12100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12100
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.052"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            134..427
                     /locus_tag="VYN69_001369"
                     /pseudo
     CDS             134..427
                     /locus_tag="VYN69_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            534..885
                     /locus_tag="VYN69_001370"
                     /pseudo
     CDS             534..885
                     /locus_tag="VYN69_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1161..2466
                     /locus_tag="VYN69_001371"
                     /pseudo
     CDS             1161..2466
                     /locus_tag="VYN69_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011680908.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(2506..3516)
                     /gene="tsaD"
                     /locus_tag="VYN69_001372"
     CDS             complement(2506..3516)
                     /gene="tsaD"
                     /locus_tag="VYN69_001372"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002896630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEV1533632.1"
                     /translation="MKDRYILAFETSCDETSVAVLKNDDELLSNVIASQIESHKRFGG
                     VVPEVASRHHVEVITACIEEALAEAGITEEDVTAVAVTYGPGLVGALLVGLSAAKAFA
                     WAHGLPLIPVNHMAGHLMAAQSVEPLEFPLLALLVSGGHTELVYVSEAGDYKIVGETR
                     DDAVGESYDKVGRVMGLTYPAGREIDELAHQGQDIYDFPRAMIKEDNLEFSFSGLKSA
                     FINLHHNAEQKGESLSTEDLCASFQAAVLDILMAKTKKALEKYPVKTLVVAGGVAANK
                     GLRERLAAEVTDVKVIIPPLRLCGDNAGMIAYASVSEWNKENFAGWDLNAKPSLAFDT
                     ME"
     gene            complement(3506..3943)
                     /gene="rimI"
                     /locus_tag="VYN69_001373"
     CDS             complement(3506..3943)
                     /gene="rimI"
                     /locus_tag="VYN69_001373"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEV1533633.1"
                     /translation="MIEIKRIQQQPDLAQAIYAVMAAVYLVSPWTLEQIQADLSQDQT
                     WYALAYDGAEVIGFLAVQENLFEAEVLQIAVKGAYQGQGIASALFAQLPTDKEIFLEV
                     RKSNQRAQAFYKKEKMAVIAERKAYYHDPVEDAIIMKREIDEG"
     gene            complement(3940..4623)
                     /gene="tsaB"
                     /locus_tag="VYN69_001374"
     CDS             complement(3940..4623)
                     /gene="tsaB"
                     /locus_tag="VYN69_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEV1533634.1"
                     /translation="MKVLAFDTSSKALSLAILEDKQVLAETTINIKKNHSITLMPAID
                     FLMESLDWTPKDLDRIVVAEGPGSYTGLRIAVATAKTLTHTLNIELVGMSSLLALVPY
                     QQEGLFVPLMDARRNNVYAGFYENAKPVMPEAHLSFEEVLEKVKGASQVTFVGEVAPF
                     VEQIQEHLPRTNYKETLPNAANLALLAWDKEAESLHDFVPNYLKRVEAEENWLKNHTE
                     SGESYIKRL"
     gene            4800..4986
                     /gene="cibA"
                     /locus_tag="VYN69_001375"
                     /pseudo
     CDS             4800..4986
                     /gene="cibA"
                     /locus_tag="VYN69_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000180803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibA"
     gene            4989..5141
                     /gene="cibB"
                     /locus_tag="VYN69_001376"
     CDS             4989..5141
                     /gene="cibB"
                     /locus_tag="VYN69_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000977347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fratricide two-peptide bacteriocin subunit CibB"
                     /protein_id="HEV1533635.1"
                     /translation="MMKDLNNYREISNKELQEIKGGFGVGVGIALFMAGYTIGKDLRK
                     KFGKSC"
     gene            5959..6192
                     /locus_tag="VYN69_001377"
     CDS             5959..6192
                     /locus_tag="VYN69_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002947111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEV1533636.1"
                     /translation="MIYKVFYQETKERSPRRETTRTLYLDIDASSELEGRITARQLVE
                     ENRPEYNIEYIELLSDKLLDYEKETGAFEITEF"
     gene            6194..7873
                     /locus_tag="VYN69_001378"
     CDS             6194..7873
                     /locus_tag="VYN69_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009729634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEV1533637.1"
                     /translation="MAYTLKPEEVGVFAIGGLGEIGKNTYGIEYQDEIIIVDAGIKFP
                     EDDLLGIDYVIPDYSYIVDNIDRVKAVLITHGHEDHIGGIPFLLKQANVPIYAGPLAL
                     ALIRGKLEEHGLLRNAKLYEINHNTELTFKNLKATFFRTTHSIPEPLGIVIHTPQGKI
                     VCTGDFKFDFTPVGEPADLHRMAALGEEGVLCLLSDSTNAEVPTFTNSEKVVGQSIMK
                     IIQGIEGRIIFASFASNIFRLQQATEAAIKTGRKIAVFGRSMEKAIVNGIDLGYIKAP
                     KGTFIEPNEIKDYPAGEVLILCTGSQGEPMAALSRIANGTHRQVQLQPGDTVIFSSSP
                     IPGNTTSVNKLINIISEAGVEVIHGKVNNIHTSGHGGQQEQKLMLRLIKPKYFMPVHG
                     EYRMQKVHAGLAVDTGVEKDNIFIMSNGDVLALTADSARIAGHFNAQDIYVDGNRIGE
                     IGAAVLKDRRDLSEDGVVLAVATVDFKSQMILSGPDILSRGFVYMRESGDLIRQSQRI
                     LFNAIRIALKNKDASVQSVNGAIVNAIRPFLYENTEREPIIIPMILTPDEE"
     gene            complement(8207..10120)
                     /gene="mnmG"
                     /locus_tag="VYN69_001379"
     CDS             complement(8207..10120)
                     /gene="mnmG"
                     /locus_tag="VYN69_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEV1533638.1"
                     /translation="MTYHFTEEYDIIVIGAGHAGVEASLAASRMGCKVLLATINIEML
                     AFMPCNPSIGGSAKGIVVREVDALGGEMAKTIDKTYIQMKMLNTGKGPAVRALRAQAD
                     KELYSKEMRKTVENQENLTLRQTMINEILVEDGKVVGVRTATHQEYAAKAVIVTTGTA
                     LRGEIIIGDLKYSSGPNHSLASINLADNLKELGLEIGRFKTGTPPRVKASSINYDVTE
                     IQPGDEAPNHFSYTSRDEDYVKDQVPCWLTYTNGTSHEIIQNNLHRAPMFTGVVKGVG
                     PRYCPSIEDKIVRFADKERHQLFLEPEGRNTEEVYVQGLSTSLPEDVQRDLVHSIKGL
                     ENAEMMRTGYAIEYDMVLPHQLRATLETKKISGLFTAGQTNGTSGYEEAAGQGIIAGI
                     NAALKIQGKPELILKRSDGYIGVMIDDLVTKGTIEPYRLLTSRAEYRLILRHDNADMR
                     LTEMGREIGLVDDERWARFEIKKNQFDNEMKRLDSIKLKPVKETNAKVEEMGFKPLTD
                     AVTAKEFLRRPEVSYQDVVAFIGPAAEELDDKIIELIETEIKYEGYISKAMDQVAKMK
                     RMEEKRIPANIDWDDIDSIATEARQKFKLINPETIGQASRISGVNPADISILMVYLEG
                     KNRSISKTLQKSK"
     gene            complement(10130..10585)
                     /locus_tag="VYN69_001380"
     CDS             complement(10130..10585)
                     /locus_tag="VYN69_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1533639.1"
                     /translation="MTQQDFRTKVDNTVFGVRATALIVQNHKLLVTKDKGKYYTIGGA
                     IQVNESTEYAVVREVKEELGVKAQAGQLAFVVENRFEQDGVSYHNIEFHYLVDLLEDA
                     PLTMQEDEKRQPCEWIDLDKLEDIQLVPAFLKTALPDWEGQLRHIHLEE"
     gene            complement(10726..11847)
                     /gene="mnmA"
                     /locus_tag="VYN69_001381"
     CDS             complement(10726..11847)
                     /gene="mnmA"
                     /locus_tag="VYN69_001381"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEV1533640.1"
                     /translation="MSDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVVAVADQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMTLGADYVATGHYARVVRDEDGTVHMLRGVDNGKDQTYFLSQLS
                     QEQLQKTMFPLGHLEKPEVRKLAEEAGLSTAKKKDSTGICFIGEKNFKNFLSNYLPAQ
                     PGRMMTVDGRDMGEHAGLMYYTIGQRGGLGIGGQHGGDNAPWFVVGKDLSKNILYVEQ
                     GFYHDSLMSTSLEASQVHFTREMPEEFTLECTAKFRYRQPDSKVTVHVKGDKAEVIFA
                     EPQRAITPGQAVVFYDGEECLGGGLIDNAYRDGQVCQYI"
BASE COUNT         3420 a         2854 c         2412 g         3414 t
ORIGIN      
        1 ctcttgtctc gaccttctcc aaagaattgc tataatacta ttacaaatac atctgcacta
       61 cacttcaaat tttagcactg tataaaaacg tttcaataca ctaacttcaa gaaaacttcc
      121 actattaatt gaaaaagttg atagagataa attaaagatc tatattgaaa ctcatcccga
      181 tgcttatttg actgaaatag ctgctgaatt caactgtcct ccaacaacta ttcattacgc
      241 tctaaaggct atgggatata gtctaaaaaa gagccgtacc tactgcgaac aagacccaga
      301 aaaagtaaat cggttcctta aagaattgaa tcacttaagc tacctgactc ctatttatat
      361 ttatgagaca ggggttgaga cctattttta tctcgaatat gatcgagcct tgagcaggca
      421 gttagtctct ctggaagaag atataattat ttgaattaag atcgagacaa cgcacaccag
      481 agattgcgat actgttatag aagtactaat gccctttttt gtttcaatat actatggctc
      541 cgatgaccta taaagatacg atgacgagtg actttttcga agcttgcttc caaaaattct
      601 tactacctac tttagataca ccatccctta tcattatgga caatgcaagg tttcacagaa
      661 tgaacatgtg taaggagcag ggcatagact gttaccactt cctacctatt cacccgagta
      721 taatcccatt gagaaaatat gggcttacat caaaaacatc tcagaataat attgtcaaat
      781 tacgatgctt ttcttgaggc acttttgtcc tattcttgtt tcagccgact atgctccgtt
      841 attgggcagc tacggaacag tcgatgggac gatgggggga cataaaaaaa tcctccagtt
      901 ttgtttttta taacagtata ctggagaatt gacaatctcg gtagatacct cgttatagcg
      961 cggttactta ttaggcagtt acaaaacaac tgtgaacaga aaacattcca gagtcagaca
     1021 agactttgga atgttttggc tctataattt ctgtagtggg taatcccacc ccaggaatta
     1081 tagggtcgtt tcttgtagaa aaaaagcccc atatgaccta taatgaaaag cgtctaacca
     1141 actcattaga aagggttcat atggaacaac ttaagaatac cacagatttg ctcggattga
     1201 aagacaaaaa tatcaaaatc ttgtctgttc tgaaatacca aacccatcta gtcgttcagg
     1261 caaagttgga ttcccccgct cctccttgtc ctcattgtca agggaagatg atcaaatacg
     1321 ccttccagaa agcctctaaa attccgcttc tcgactgtca gggtttaccc acggtactac
     1381 atctcaaaaa gcgccgcttt cagtgcaaga attgccttaa ggtggtcgtt tctcaaacat
     1441 ccattgtcaa gaaaaattgc cagatttcca acatggtgag acaaaaaatc gctcagctcc
     1501 tccttgaaaa gcagtctatg actgagattg cccacagatt ggcggtctca acttccaccg
     1561 tcatccgaaa actgagggaa tttaagtttg aaaccgattg gaccaagttg ccaaaagtta
     1621 tgagttggga tgagtatagc ttcaaaaaga gcaaaatgag cttcattgcc caagattttg
     1681 agtccaaatc catcctcgca attttagacg ggcgaactta tgcggtgatt cgaaaccatt
     1741 tccaacgcta tcagagagag gttcgggagc tggtcgaggt catcaccatg gacatgtaca
     1801 gcccttatta tcggctcgct aagcaactct ttccaaaggc gaagattgtt cttgaccgct
     1861 tccacattgt ccaacatctg agccgagcta tgaaccgagt acgaatccaa atcatgaacc
     1921 aatttgaccg aaaatcctta gagtatcggg cgctcaagcg cttttggaac cctcgctttt
     1981 tcgtttctag gctcgggcta aatcagtcca ctggactgat ttactacacc aggatagctt
     2041 caagctctgt cagaaacgat tctatcagcc cacgtttcaa tgcacttaac ccatcgggaa
     2101 gtacgagata agctgctttc ttactctgag ggattacagg ttcactacga actctatcaa
     2161 ctcctgctct ttcattttca agagaagaat gccgaccatt tctttggatt gattgagcaa
     2221 gaactgccaa cggttcatcc gctttttcaa acggtctttt ggactttttt aagggataga
     2281 gataagatta tcaacgcact taagctgcct tattccaacg ctaaacttga agcgaccaat
     2341 aatttgatta aggttatcaa gcgcaaaacc tttggtttcc ggaactttaa caattttaaa
     2401 aaacggattt tgatgacttt gaacatcaaa aaagagagta cgaatttcgt actctccaga
     2461 ttgtagcttt tcgcctaccc actacacttg acaaagagcc actctttatt ccatggtatc
     2521 aaaggcaaga cttggtttgg cattgaggtc ccagcctgcg aagttttctt tgttccactc
     2581 gctgacactg gcataagcaa tcatacctgc attgtctccg cagaggcgca gaggtggaat
     2641 gatgaccttg acatctgtaa cctcggctgc taggcgttct ctgagacctt tattggctgc
     2701 cacaccacct gccacaacca gggttttaac aggatatttc tccaaagcct tcttggtttt
     2761 tgccatgaga atatccagta cagctgcttg gaaggaagca cacaaatcct ctgtagacag
     2821 actttctccc ttttgctcgg cattgtgatg aagattgata aaggcagatt tcaaacctga
     2881 gaaggagaac tccagattat cttccttaat catggcacgg gggaaatcat aaatatcctg
     2941 cccctgatga gccagctcgt caatctcacg acctgcagga taggtcaagc ccatgacacg
     3001 accgacctta tcataggact caccaaccgc gtcatctcgc gtttccccaa caatcttgta
     3061 atcgccagcc tcagaaacat agaccaactc tgtgtgccca ccactgacta aaagggctag
     3121 caagggaaac tccaaaggct ccacactctg agctgccatg aggtgcccag ccatgtgatt
     3181 aacaggaatc agtggaagtc cgtgagccca agcaaaggcc ttggcagctg acaaaccaac
     3241 tagcaaggct ccgaccaagc ctggtccgta ggtaaccgca acagctgtca cgtcctcttc
     3301 ggtaatccct gcttctgcca atgcctcctc gatacaggct gtaatgacct cgacatggtg
     3361 acgactggct acttcgggca ctacgccacc aaaacgtttg tgactctcaa tttgactagc
     3421 aatgacattg gacaagagct catcgtcgtt tttcaagacg gcgacactgg tctcatcaca
     3481 ggatgtctca aatgctaaaa tatatctatc cttcatctat ttctctcttc atgataatgg
     3541 cgtcctcgac tgggtcatgg tagtaggcct ttcgctcagc gatgaccgcc attttttctt
     3601 tcttgtaaaa tgcttgcgct cgttgatttg actttctgac ttcgaggaaa atctccttat
     3661 ctgtcggcaa ttgagcaaac aaggctgacg caattccctg accctgataa gctcctttga
     3721 cagcgatttg caggacttct gcttcaaaaa gattctcctg cacagctaga aatccaatca
     3781 cttctgcccc atcataagcc aatgcatacc aagtctggtc ttgggacaga tctgcttgga
     3841 tttgctccag agtccaagga ctgactaggt aaacagctgc cataacagcg tagatggctt
     3901 gagctaggtc aggctgttgt tgaattcgct tgatttctat cataggcgtt taatgtagga
     3961 ctcaccagac tcggtatggt tcttgagcca gttttcctca gcctcgactc gtttgaggta
     4021 atttggcaca aaatcatgca aggattctgc ttccttgtcc caagctaaaa gagcaagatt
     4081 ggctgcatta ggcaaggttt ctttataatt agtccttggc aagtgttctt gaatctgctc
     4141 cacaaagggg gcaacttctc cgacaaaggt tacctgacta gcacccttga ctttttctag
     4201 cacctcttca aaagataggt gcgcttctgg catgacaggt ttagcatttt cataaaatcc
     4261 tgcataaaca ttgttgcgac gcgcatccat caaaggaacg aacaagcctt cttgttgata
     4321 tggcaccaga gccaagaggc tagacatacc aactaactca atgttcagag tatgagtcaa
     4381 ggtcttggca gtggctaccg cgattcgcaa gcctgtgtag ctacctggcc cttccgccac
     4441 cacgattcgg tccaaatcct tgggcgtcca gtccaaactc tccatcaaaa aatcaatggc
     4501 tggcatgagg gtaatactgt gatttttctt gatattaatc gtcgtctcag caagaacctg
     4561 cttgtcctct aaaatagcca gagaaagagc cttgctggac gtatcaaaag ctaatacttt
     4621 cataacacat tcctatcttt ttgtctgctt actattatac tacaaaagct ggcacatggg
     4681 aattttcttt gcccccagac aagagtgccc tcacttaact aaaaataatt taaaaaatgc
     4741 tcacttttcc ttttcttttc cgaatataaa agtgaacaag aaaaaaggag gaaagttcaa
     4801 tgacaaattt tgacattctt gacaatcaat tttttatcct tatctgaaaa tgaattatca
     4861 gatattgatg gcggtctcgc tcccttggtt atctttggag tagcagtatc ttggaaggct
     4921 attgcaggtg gaacagcact tataggttct ggtttggcag ctggttattt tttaggagga
     4981 gattaactat gatgaaagat ttgaacaact atcgtgaaat ttctaataag gaattgcaag
     5041 aaatcaaggg tggctttggt gtaggtgttg gtatcgcttt atttatggca ggttatacca
     5101 ttggaaaaga ccttcgtaaa aagtttggta agtcatgcta gataagaaac acatttttag
     5161 aaggataaat tttattgtct tcatctctta cagtttgctc agcattctca atgatttgaa
     5221 cattactact atccctttac cattcgattt atctgtttgt attgttttat ttttatgctt
     5281 caactctatt tttgagcaga acaatgactc ccataaaaat aataagcttt gaaaattcca
     5341 ttgtcatgtc atgttagaaa aatgcaaaga ccacctcatc ttgatagatg gggtggaatt
     5401 ttcgtgtcgt aaatctacta tctctacatc cccaaacaaa aaaccccagc ataagcaggg
     5461 catctaagca tttaatccaa agtaaaatac aaaccaaacg acataggtca cgaggaggag
     5521 aaaaagcgag tagagagtca caaaggtaat tttccacaag aacttggttt gtcatcgttc
     5581 cagtttggca aatagaagat tccccgcata aacgcaagca acaaaaacaa taaaagctac
     5641 caagcgagct ccgatagcaa aagcaaataa gttatacata gggcaacctc cttgacttaa
     5701 aatctatatg gaattatgac aagcaataaa tttcacttcc gttatcaaca taatacattt
     5761 tctttatttt tgcaaacgct taccaaagaa atcgtcccct aactttctcg tttccgtctt
     5821 ttactaattt ttcattttgt ggtataattg aaataattgt aacgaatcaa ggtcaatcta
     5881 gacacaaaat ggaatgaaat caagcaaata tctgctaaaa gtttggaata agctgacctg
     5941 taaatagaaa ggaactatat gatttacaaa gttttttatc aagaaacaaa agaacgtagc
     6001 ccacgccgtg aaacaacacg cacgctttac ctagacatcg atgccagctc agaacttgag
     6061 ggccgtatca ctgctcgcca acttgtcgaa gaaaatcgcc cagagtacaa tatcgagtat
     6121 atcgaactct tgtctgacaa attgctcgat tacgaaaaag aaactggcgc cttcgaaatt
     6181 acggagttct aatatggcct acactcttaa acctgaagaa gtcggcgttt ttgccatcgg
     6241 tggtctagga gaaatcggga aaaacactta cggaattgaa taccaagacg agattatcat
     6301 cgtcgatgct gggattaaat tcccagaaga tgacttgctt ggtatcgact atgtcattcc
     6361 tgactactct tacatcgtgg acaatatcga ccgcgtcaag gctgttttaa tcacacacgg
     6421 acacgaggac cacattggtg ggattccgtt cctactcaag caagcaaatg tccctatcta
     6481 cgctggaccg cttgccttgg ctttgatccg tgggaaactc gaagaacacg gcctcttgcg
     6541 caacgccaaa ctttacgaaa tcaaccacaa caccgagttg acctttaaaa atctcaaggc
     6601 aactttcttt agaacgactc actctattcc agagcctttg gggattgtca ttcatactcc
     6661 tcaagggaaa atcgtctgta cgggtgactt taagttcgac tttactccag ttggagaacc
     6721 tgcggacttg catcgtatgg ctgcccttgg tgaagaaggc gtgctctgtc tcctgtctga
     6781 ctcgacaaat gcggaagtac caacctttac caactctgaa aaagtcgttg gtcagtccat
     6841 tatgaagatt atccaaggta ttgaaggacg tatcatcttt gcatcctttg cctcaaatat
     6901 cttccgtctc cagcaggcaa cagaagctgc tattaagact ggacgcaaga ttgcggtctt
     6961 tggtcgttct atggaaaagg ccattgtcaa cggaatcgat cttggctaca tcaaagctcc
     7021 taagggaacc tttatcgagc caaatgaaat caaagattat cctgcaggag aagttcttat
     7081 cctctgtaca ggtagtcagg gtgagcctat ggcagccctc tctcgtatcg ccaacggaac
     7141 ccaccgtcaa gtacaattac aaccaggtga taccgttatc ttctcttcta gtcccatccc
     7201 tggaaacact actagcgtca acaagctgat taacatcatt tctgaagctg gtgtcgaagt
     7261 tatccacggt aaagtgaaca atatccatac atctggacac ggtggccagc aagagcaaaa
     7321 actcatgctc cgcttgatta agccaaaata cttcatgcct gtccacggtg aataccgcat
     7381 gcaaaaagtc cacgctggac tagcagtgga tactggtgtt gagaaggaca atatctttat
     7441 catgagcaat ggcgatgtgc ttgcccttac tgctgactca gctcgtatcg caggtcattt
     7501 caacgcccaa gatatctatg tcgatggaaa tcgtatcggt gaaattggcg cagctgtcct
     7561 caaagatcgt cgcgatctat ctgaagacgg tgtcgttcta gcagtcgcaa ctgttgactt
     7621 caaatcgcag atgattctgt ctggcccaga tatcctcagc cgaggctttg tctacatgag
     7681 agagtctgga gacttgattc gccaaagcca gcgtatcctc ttcaatgcca ttcgtatcgc
     7741 actgaaaaat aaggatgcta gcgtgcaatc tgtcaatggt gccattgtca acgctattcg
     7801 ccccttcctc tatgaaaata ccgaacgtga accgatcatc atcccgatga tcctcacacc
     7861 agatgaagaa taatactctt caaaccacgt cagcttcgcc ttgccgtata tatgttactg
     7921 acttcgtcag ttctatccac aacctcaaag cagtgctttg agcaacctac ggctagcttc
     7981 ctagtttgct ctttgatttt cattgagtac aaatcaacaa aacagccccg tcttcggagc
     8041 tgtttttctc tatgctttct ttttagatta aaactcaatg aaaatcagac cagaaggcta
     8101 ggaaaatgca taacttgagt tagctcccat agttcgggga actatgggag attggagatg
     8161 aatcaaagcc aagctttgaa ctcattcgta agaagcctac gacatatcat tttgattttt
     8221 gaagagtttt agaaatacta cgatttttac cttccagata caccatcaaa atagaaatat
     8281 ctgctgggtt tactcccgaa atacggctgg cttggccgat ggtttctgga ttgatgagtt
     8341 tgaacttctg acgggcttcg gttgcgatag aatcaatgtc atcccagtcg atattggctg
     8401 gaatacgttt ttcttccatg cgcttcatct tagcaacctg atccatggct tttgagatgt
     8461 aaccttcgta cttgatttct gtttcaatca attcgataat cttgtcatct aattcctctg
     8521 cagctggtcc gatgaaggcc accacatctt ggtaagaaac ttctggacgg cgaaggaatt
     8581 ccttggctgt caccgcatct gtcaacggct tgaagcccat ttcctcaacc ttagcattgg
     8641 tttccttgac tggcttgagt ttgatactgt ctaggcgctt catctcatta tcaaattgat
     8701 ttttcttgat ttcaaaacga gcccagcgtt catcgtccac aaggccaatc tcgcgtccca
     8761 tctcagtcaa gcgcatatca gcattgtcat ggcgaagaat gagacgatat tcagcacgac
     8821 tggtcaagag acggtaaggt tcaatggttc ccttggtcac caagtcgtcg atcatcaccc
     8881 cgatataacc gtcacttcgt tttagaatca attcaggctt gccttggatt ttcaaagccg
     8941 cattgatacc cgcgataatc ccttggccag cagcttcttc ataacctgat gttccatttg
     9001 tctgaccagc agtgaagaga cctgagattt tcttggtttc caaagtcgca cgcaactgat
     9061 gaggcaagac catgtcgtac tcaatggcat aacctgttcg catcatctct gcattttcca
     9121 aacctttaat ggaatgaacc aagtcacgct ggacatcctc aggtagactg gttgagaggc
     9181 cttgcacata gacttcctct gtattacgtc cttctggctc aaggaagagt tggtggcgtt
     9241 ccttgtccgc aaagcgcaca atcttgtctt caatcgacgg acagtaacga ggccccactc
     9301 ccttgaccac acctgtaaac ataggcgcac ggtggaggtt gttttggata atctcatgac
     9361 tggtaccatt ggtgtaggtc aaccagcatg gcacttggtc cttgacataa tcctcatcac
     9421 gtgaagtgta tgagaagtga ttaggcgctt catctcctgg ttggatttct gtcacatcgt
     9481 aattgataga agaagccttg acacgtggag gggttcctgt cttgaaacga ccgatttcga
     9541 ggcccagttc cttgagattg tcagctaggt taatagaagc caagctgtgg ttaggacctg
     9601 atgagtactt gaggtctccg atgataattt ccccacggag agcagtccct gtcgtcacaa
     9661 taacagcctt agcagcatat tcttgatggg tggctgtacg cacaccgaca accttgccat
     9721 cttccaccaa aatctcatta atcatggttt gacgaagggt cagattttct tgattttcaa
     9781 ctgtcttgcg catctcctta gagtaaagtt ccttgtcagc ctgcgcacga agagcacgga
     9841 cagctggccc cttccctgtg ttgagcatct tcatctggat gtaagtcttg tcaatggtct
     9901 tggccatctc gccaccgagg gcatcgactt cacgtacgac aatcccctta gcagaaccac
     9961 cgatagaggg attacaaggc atgaaagcca gcatttcaat gttaatagtc gcaagcagga
    10021 ccttacagcc catacggcta gcagctaagg aagcctcaac cccagcgtgt cccgcaccaa
    10081 ttacaataat atcgtattct tcagtaaaat gataagtcat gtttctctcc tattcttcaa
    10141 gatgaatgtg tcttagttgg ccttcccaat ctggtagggc cgtttttaaa aaggctggaa
    10201 ctagctggat atcctcaagc ttatccaagt caatccactc acagggctgc cttttctcat
    10261 cttcctgcat ggtcaacggg gcatcttcaa gcaaatccac cagataatga aactcgatat
    10321 tgtgatagga aacgccgtct tgttcaaaac gattttcaac cacaaaagct agctgcccag
    10381 cttgagcttt gacacccagt tcttccttca cttcacggac taccgcgtat tccgtgcttt
    10441 cattgacttg aatcgcacct ccaatagtgt aatacttgcc cttgtctttg gtaactagaa
    10501 gcttgtgatt ttggacaatc aaggctgtag cccgaacacc aaaaaccgta ttgtctactt
    10561 ttgtccgaaa gtcttgttga gtcattcttg tcctttccct taaacgacac aaaaacagtc
    10621 aaaactacaa agaagtgcag gacaaaaaag cctgcaacat ccatgagctt tgaccatcat
    10681 ttctattgct gttattattg tagcaaattg agaaaatttg tcaatctaaa tgtactgaca
    10741 aacttgtcca tcgcggtaag cattgtcaat caaaccgcca cctagacact cttcgccatc
    10801 gtaaaagaca actgcctgtc ctggtgtaat cgcgcgttgt ggttctgcaa agataacttc
    10861 tgccttgtct cctttgacat ggacggtcac cttagagtca ggctgacggt aacggaattt
    10921 agccgtacat tctagcgtaa actcttctgg catttcacga gtaaagtgga cttgactggc
    10981 ttctaggcta gttgacatga gcgagtcatg gtagaatcct tgttctacat agagaatatt
    11041 cttgcttaga tcttttccga caacgaacca aggggcattg tcaccaccgt gttgcccacc
    11101 gataccgagt ccgccacgct gaccgattgt atagtacata agacctgcat gctcgcccat
    11161 atcgcgacca tccacagtca tcatacgacc aggctgagct ggcaggtagt tgctgagaaa
    11221 gtttttaaag ttcttttctc cgataaagca aatccctgtc gagtctttct tcttagcagt
    11281 cgaaagacct gcttcttctg ctagttttcg aacttcaggc ttttccaaat gtcctagtgg
    11341 gaacatcgtt ttttgaagtt gttcttgcga aagttggctg aggaaatagg tctgatcctt
    11401 gccattgtcc acgccacgaa gcatgtgaac ggtaccatcc tcatcacgca ccactcgagc
    11461 ataatgccca gtcgctacat agtctgcccc caaggtcata gcatagtcca aaaaggcctt
    11521 gaacttgatt tccttgttgc acataacgtc cggatttggc gtgcgtcccg cacggtattc
    11581 cgctaggaaa tactcaaaaa cgcggtccca gtactctttt tcaaaattga cagagtagta
    11641 gggaatgcca atctggtctg ccaccgcaac cacatccttg taatcttcgg tcgccgtaca
    11701 gacgccgttt tcatctgtgt catcccagtt cttcatgaag ataccgatca catcgtagcc
    11761 ctgctccttg agcaaaagag ccgtcaccga cgaatcaaca ccaccactca tccccacgac
    11821 aacacgtgtt ttagagttat cactcatggt aagtctccca tctattcgtt tattcacgat
    11881 tgaaggtcgt gtgtgcttca cgattgaagg tcgcttgagc agtattcatt ataacacgct
    11941 tggttagaga agacaaggaa gagcttaaaa attgtttttt aaaacttata gtcaatatac
    12001 agatcatgtg aatgacgtga gtataccttc ttcttatctt cattcaaata actataataa
    12061 ataaaacaac ccattttctc taatctattt ttcaagttag
//
LOCUS       DAVLVP010000053        11871 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.053, whole genome shotgun sequence.
ACCESSION   DAVLVP010000053 DAVLVP010000000
VERSION     DAVLVP010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11871
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.053"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            1..404
                     /locus_tag="VYN69_001382"
                     /pseudo
     CDS             1..404
                     /locus_tag="VYN69_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476106.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
     gene            367..1545
                     /locus_tag="VYN69_001383"
                     /pseudo
     CDS             367..1545
                     /locus_tag="VYN69_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014678337.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            complement(1808..2200)
                     /gene="rpsI"
                     /locus_tag="VYN69_001384"
     CDS             complement(1808..2200)
                     /gene="rpsI"
                     /locus_tag="VYN69_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEV1533641.1"
                     /translation="MSQAQYAGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     RLVINQPFAVTSTVGSYDVFVNVVGGGYAGQSGAIRHGIARALLQVDPDFRDSLKRAG
                     LLTRDSRKVERKKPGLKKARKASQFSKR"
     gene            complement(2220..2666)
                     /gene="rplM"
                     /locus_tag="VYN69_001385"
     CDS             complement(2220..2666)
                     /gene="rplM"
                     /locus_tag="VYN69_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003083327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEV1533642.1"
                     /translation="MNKTTFMAKPGQVERKWYVVDATDVPLGRLSAVVASVLRGKNKP
                     TFTPHTDTGDFVIVINAEKVKLTGKKATDKIYYTHSNHPGGLKQISAGELRSKNAVRL
                     IEKSVKGMLPHNTLGRAQGMKLKVFVGAEHTHAAQQPEVLDISGLI"
     gene            3023..3340
                     /locus_tag="VYN69_001386"
     CDS             3023..3340
                     /locus_tag="VYN69_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEV1533643.1"
                     /translation="MAFTNTHMRSASFGIVTSLPDDIIDSFWYIIDHFLKNVFELEEE
                     LEFQLLNNQGKITFHFSSQHLPTAIDFDFNHPFDPRYPPRVLVLDMDGRETILLPEEN
                     DLF"
     gene            complement(3491..4303)
                     /gene="folK"
                     /locus_tag="VYN69_001387"
     CDS             complement(3491..4303)
                     /gene="folK"
                     /locus_tag="VYN69_001387"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HEV1533644.1"
                     /translation="MDQLQIKDLEMFAYHGLFPSEKELGQKFVVSAILSYDMTKAATD
                     LDLTASVHYGELCQQWTTWFQETSEDLIETVAYKLVERTFESYPLVQEMKLELKKPWA
                     PVHLSLDTCSVTIHRRKQRAFIALGSNMGDKQANLKQAIDKLRARGIHILKESSVLAT
                     EPWGGVEQDSFANQVVEVETWLPAQDLLETLLAIESELGRVREVHWGPRLIDLDLLFV
                     EDQILYTDDLILPHPYIAERLFVLESLQEIAPHFIHPILKQPIRNLYDALKK"
     gene            complement(4346..4900)
                     /gene="folE"
                     /locus_tag="VYN69_001388"
     CDS             complement(4346..4900)
                     /gene="folE"
                     /locus_tag="VYN69_001388"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006155259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HEV1533645.1"
                     /translation="MDTQKIEAAVKMIIEAVGENANREGLQETPARVARMYQEIFSGL
                     GQTAEEHLSKSFEIIDDNMVVEKDIFFHTMCEHHFLPFYGRAHIAYIPDGRVAGLSKL
                     ARTVEVYSKKPQIQERLNIEVADALMDYLGAKGAFVVIEAEHMCMSMRGVRKPGTATL
                     TTVARGLFETDKDLRDQAYRLMGL"
     gene            complement(4881..6203)
                     /locus_tag="VYN69_001389"
     CDS             complement(4881..6203)
                     /locus_tag="VYN69_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000658404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEV1533646.1"
                     /translation="MKEIENNQWIANYRTDQPHFGLERMVELLALRGNPHLKLKVLHI
                     GGTNGKGSTIAFLKKMLEKLGLRVGVFSSPYLIHYTDQISINGESISEARLEALMADY
                     QSLLEGEAVANLQGTTEFEIITALAYDYFASEQVDVAIMEVGMGGLLDSTNVCQPILT
                     GITTIGLDHVALLGDTLESIAEQKAGIIKQGMPLVTGRIAPEALAVIDRIAEEKDAPR
                     LAYGTDYQVRHQESVVTGEVFDYTSAVRQGCFQTSLLGLYQIENAGMAIALLDTFCQE
                     DGRELASNDFLGQALEETSWPGRLEIVSRDPLMILDGAHNPHAIKALLVTLQERFADH
                     HKEILFTCIKTKALEDMLDLLGAMPDTELTLTHFADSRATDESVLKEAAKARNLSYQD
                     WHDFLEQNLTDKKEEKQTVRIITGSLYFLSQVRAYLMERKNENGYTKD"
     gene            complement(6205..7179)
                     /gene="folP"
                     /locus_tag="VYN69_001390"
     CDS             complement(6205..7179)
                     /gene="folP"
                     /locus_tag="VYN69_001390"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HEV1533647.1"
                     /translation="MMKILEGVTDMSSKANHAKTVICGIINVTPDSFSDGGQFFALEQ
                     ALQQARKLIAEGASMLDIGGESTRPGSSYVEIEEEIQRVVPVIKAIRKESDVLISIDT
                     WKSQVAEAALAAGADLVNDITGLMGDEKMAHVVAKAGAKVVIMFNPVMARPQHPSSLI
                     FPHFGFGQTFTEKELADFETLPIEDLMVAFFERALARAAEAGIAPENILLDPGIGFGL
                     TKKENLLLLRDLDKLHQKGYPIFLGVSRKRFVINILEESGFEVNPETELGFRNRDTAS
                     AHVTSIAARQGVEVVRVHDVASHRMAVEIASAIRLADEAENLDLKQYK"
     gene            complement(7274..7981)
                     /locus_tag="VYN69_001391"
     CDS             complement(7274..7981)
                     /locus_tag="VYN69_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1533648.1"
                     /translation="MKEKNMWKELLNRAGWILVFLLAVLLYQVPLVVTSILTLKEVAL
                     LQSGLIVTGISIVVLVLFIIGARKTQLASFNFSFFRAKDLARLGLSYLVIICSNILGS
                     ILLQLSNETTTANQSQINDMVQNSSLISSFFLLALLAPICEEILCRGIVPKKIFRGKE
                     NLGFVVGTIVFALLHQPSNLPSLLIYGGMSTVLSWTAYKTQRLEMSILLHMIVNGIAF
                     CLLALVVIMSRTLGISV"
     gene            complement(8034..9452)
                     /locus_tag="VYN69_001392"
     CDS             complement(8034..9452)
                     /locus_tag="VYN69_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEV1533649.1"
                     /translation="MDKLFKLKENGTDVRTEVLAGLTTFFVMSYILFVNPQILSQTGM
                     PAQGVFLATIIGAVAGTLMMAFYANLPYAQAPGMGLNAFFTFTVVFGLGYSWQEALAM
                     VFICGIISLIITLTNVRKMIIESIPNALRSAISAGIGVFLAYVGIKNAGLLKFTIDPG
                     NYTVVGEGADKAQATIAANSSAVPGLVSFNNPAVLVALAGLAITIFFVIKGIKGGIIL
                     SILTTTVLAIAVGLVDLSSIDFANNHVGAAFEDLKTIFGAALGSEGLGALVSDTARLP
                     ETLMAILAFSLTDIFDTIGTLIGTGEKVGIVATNGENHQSAKLDKALYSDLIGTTVGA
                     IAGTSNVTTYVESAAGIGAGGRTGLTALVVAICFAISSFFSPLLAIVPTAATAPILII
                     VGIMMLGSLKNIHWDDMSEAVPAFFTSIFMGFSYSITQGIAVGFLTYTLTKLVKGQVK
                     DVHAMIWILDALFILNYISMAL"
     gene            9642..10454
                     /locus_tag="VYN69_001393"
     CDS             9642..10454
                     /locus_tag="VYN69_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1533650.1"
                     /translation="MTKKIIAVDLDGTLLNSDSQISDFTKKTIKKVAEKGHQVIITTG
                     RPYRMSKDFYRELGLDTPMINFNGSLTHLPDQVWDFEKCLTVDKKYLLDMVQRSEDIQ
                     ADFIAGEYRKKFYITNPNEEIANPKLFGVEAFQPEDQFQPELVTKDPNCILLQTRASD
                     KYALAKDMNAFYQHQLSINTWGGPLNILECTPKGVNKAFALDYLLKIMNRDKKDLIAF
                     GDEHNDTEMLAFAGKGYAMKNANPELLPYADEQISLTNDQDGVAKTLQDLFL"
     gene            10793..11812
                     /gene="adhP"
                     /locus_tag="VYN69_001394"
     CDS             10793..11812
                     /gene="adhP"
                     /locus_tag="VYN69_001394"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HEV1533651.1"
                     /translation="MKAVVVNPESTGVAIEEKVLRPLETGEALVEVEYCGVCHTDLHV
                     AHGDFGQVPGRVLGHEGIGIVKEIAPDVKSLKVGDRVSVAWFFEGCGTCEYCTTGRET
                     LCRTVKNAGYSVDGGMAEQCIVTADYAVKVPDGLDPAQASSITCAGVTTYKAIKEAKV
                     EPGQWVVLYGAGGLGNLAVQYAKKVFNAHVIAVDINNDKLALAKEVGADIVINGLEVE
                     DVAGLIKEKTDGGAHSAVVTAVSKVAFNQAVDSVRAGGRVVAVGLPSEMMELSIVKTV
                     LDGIQVIGSLVGTRKDLEEAFQFGAEGLVAPVVQKRPVEDAVAIFDEMEKGQIQGRMV
                     LDFTH"
BASE COUNT         3646 a         2578 c         2277 g         3370 t
ORIGIN      
        1 tacatacaaa ccgcctttat acaacacata aagatagctc aggatatgaa aaaatctcta
       61 ggagcgcaat cttacaatac gagttagaca aacaaaaggg gcatatcttt atcaaagctc
      121 tgattactgg tgatgaattt agtaaaaggg tacaatcact tgatgatgtt cttgctggag
      181 tactcgaact ggaactagaa tacaaaatta ttcgtcctag ttttatggaa taccatttct
      241 attacaagaa acctgacagg ttagttcttc aatctcacaa ccagaagcaa gagatagata
      301 accttaatat agatttagga tacggtgtga attataatcc tatcaagtgt cctcatattc
      361 tagttgctgg aggtacagga agcgggaaat cagtatttat atctttaagg aaatcacttt
      421 gttaaacgct ataaaacaag aaaggaggct aaagaagctg aattagaaat tctaacaaag
      481 atacatcaac ttgaaaatgg agaggatatc ccactcctta aaactagtga tattcttttc
      541 tctgagttct ttaataatat ttggtgggat tcatacaaag ctggtcaaac aacttcaaca
      601 actaaacccc caacacaagt tactattgat aatactgaaa tagttttcag gaagcacatc
      661 ttaccaatgt tcggtaatta ttcaatcaat tttctgaatc agaataagca aatcgtacta
      721 aacttaatga ctgctaaagc tgaagaatat gctaatttca aggttattcg aagctatgta
      781 aactcaattt tcgattgggc tgaagaatta gaatacattg aatcaaatag attatcaaaa
      841 agcataaaac gtattaaagc aagtaaaaaa ataaagctag aagaagctaa ggatgaggaa
      901 gatttgtatc tatctagtga agaattacaa gattggtttg atgcttttag agaagatttg
      961 gagaacgaca aattatcact aaaagattat gtcctattct ttacaacctt tattttgagc
     1021 gatagaaaat cagaatccta tgctctacat tggaaaaaca tagacttggc aaacgctcaa
     1081 attggcttaa ggcatgcact tgacaaatat aagaatgtaa agtcaacaaa aggaaataag
     1141 aaaacgattt tttcaattcc tagttacctt gtatcacttc tttcagaatg gaaaaaacag
     1201 cagaaatatg agttagcgaa atttggtata atgcaaacat ccgatcaact agtctttacc
     1261 tatatagata cgaaaggtaa tgtaaatagc cctctacacg ttgattatct caataacaaa
     1321 atgaactctg taaaaagacg acacccaaag ttaaaacacg ctacacctca taaattacga
     1381 catacaggag cgacacttgc taaaaaggca ggaatgagct tagaagccat ttctgaagta
     1441 ttaactcata gtgatacaat tacaactaaa acttatgtta atgcttcaaa catagttcca
     1501 ttatctgctg gacaagttgc ctaccaacat cttaaaaaca agtagggtaa ttttagggca
     1561 aattttaggg aaaacttttt tagaaagtat agaaaaaagc acccataggg caaacctttg
     1621 agtgctttga aacattgata taatcgtatt gattaacgtt ttgaggcaac tgaccttgtc
     1681 aggttgcagc cacatttgta agcgactaaa gtcgcaacaa ctgtgtcaat tgcaccaatt
     1741 tagtaagaaa gtataaaaaa agaacacccc gaaaggtgct ctgtataagt atagtaattc
     1801 tttcgaatta acgtttacta aattgtgatg ctttacgagc tttcttaaga cctggtttct
     1861 tacgttcaac tttacgtgag tcacgtgtaa gaagtcctgc gcgtttcaat gaatcgcgga
     1921 agtctgggtc tacttgaaga agggcacgag cgataccgtg acggatagct cctgattgac
     1981 cagcgtatcc accacctaca acgttaacga aaacgtcgta tgaacctaca gttgaagtaa
     2041 ctgcgaatgg ttggttgatg acaagacgaa ggtcagcgtg tgggatgtac tcttcaacat
     2101 cttttttgtt aacagtgatt ttaccagttc ctggaacaag gcgaacgcgt gcaacagcgt
     2161 ttttacgacg tccagtacct gcatattgtg cttgtgacat actttatttg ttcctttcct
     2221 tagataagtc ctgaaatgtc aagaacttct ggttgttgtg cagcatgagt gtgctcagct
     2281 ccaacaaata ctttcaactt cataccttga gcgcgtccaa gtgtattgtg tggaagcata
     2341 cctttaactg atttctcaat caaacgtact gcatttttag aacgaagttc acctgcagag
     2401 atttgtttca atccacctgg gtggtttgag tgagtgtagt agattttatc agttgctttt
     2461 ttaccagtca atttaacttt ttcagcattg ataacaatca cgaagtcacc tgtatcagtg
     2521 tgtggtgtaa atgttggttt gttttttccg cgaagtacgc tagcaactac tgcagaaaga
     2581 cgtccaagtg gtacatcagt tgcgtcaact acgtaccatt tacgttcaac ttggcctggt
     2641 ttagccataa atgttgtttt gttcatgatt tctcctatat atagttcgat ttttgtttac
     2701 ggtgatgggt gttccttccc atcgaaaagt atttggaagg ttccggggcc tttcaaatgg
     2761 ggtaaacaat accgcctact atcataccaa attttacccc taaaagtcaa tagatatgaa
     2821 aaatattttt cggtaacttc tcaaagtaac tgaaagttat ataatcaagt taatttggga
     2881 atttacctat taacttcggt agatttttgg gaattttttt ccttcttttt ccctgtagtt
     2941 aaaatccttt tcaaaaaatc accttttctt gatttttaat cctatttgct gtatgataag
     3001 ggaaaagaaa ggggacagag atatggcttt taccaatacc cacatgcggt ctgctagttt
     3061 tggcattgtt accagcttgc ccgatgacat cattgactct ttttggtata tcatcgatca
     3121 tttcttaaaa aatgtctttg aattggaaga agaactcgag tttcaattgc ttaataacca
     3181 aggaaagatt accttccact tttcaagtca acacctccct acagccattg attttgactt
     3241 taaccatcct ttcgaccctc gttatccccc aagagtactg gttttagaca tggacggtag
     3301 agaaactatc ctcctcccag aagaaaatga cctattttaa tactcaatga aaatcaaaga
     3361 gcaaactagg aagctaaccg caagctgtac ttgagtacgg taaggcgacg ctgacgtggt
     3421 ttgaatttga ttttcgaaga gtataaaaac tctagccttc agttgcaagt gactgaaaac
     3481 tagagttttt ctattttttc aaagcatcat acaagttgcg gatcggttgt tttaatatcg
     3541 gatggataaa atgaggcgca atttcctgta aggactcaag gacaaaaagg cgttccgcta
     3601 tgtaaggatg aggcaatatg aggtcgtctg tataaaggat ctggtcctcc acaaagagca
     3661 agtccaaatc aatcaaacga ggtccccaat gcacttctct cacccgtccc agctctgact
     3721 caatggctaa caaggtttct aacaagtctt gtgctggtag ccaggtttcc acctcaacca
     3781 cttgattggc aaagctatcc tgctccactc caccccaagg ctccgtcgct aagacactgg
     3841 actctttgag aatatggatg ccacgagctc gcagtttgtc aatggcttgc ttcaagtttg
     3901 cttgtttatc tcccatattg cttcctaggg cgataaaggc tcgttgcttg cggcgatgaa
     3961 tggttaccga gcaagtatct agtgacaaat gcaccggtgc ccaaggtttt ttcagttcca
     4021 acttcatttc ttggacaaga ggataagact caaaggtacg ttccaccagt ttataggcta
     4081 ccgtttcaat caaatcttca ctcgtttcct gaaaccaagt cgtccactgc tgacacaatt
     4141 ctccgtaatg gacagaggct gttaaatcca agtctgtagc tgccttggtc atatcatagg
     4201 ataggatggc tgaaacgaca aatttctgcc ccaattcttt ctcactagga aaaagaccat
     4261 gataggcaaa catttccaaa tccttaatct gcagttgatc cataatgatt cctttctaga
     4321 aaaatccgct tgaagcggat tctttttata gccccattaa acgataagct tggtcacgga
     4381 gatccttatc tgtttcaaat agaccacgag ctactgtcgt caaggttgca gtgcctggtt
     4441 ttctaacacc acgcatactc atacacatat gttccgcctc aatgacaaca aaggctcctt
     4501 tagcacctag atagtccatc aaggcatctg ccacttcgat attcaaacgt tcttgaattt
     4561 gtggtttttt cgaataaact tcaaccgtac gggctagctt agacaagcct gccacacgac
     4621 catctggaat gtaggcaatg tgcgctctac cataaaatgg caagaagtgg tgttcacaca
     4681 tggtatggaa aaagatatcc ttttctacca ccatattatc gtcaataatt tcaaaggatt
     4741 ttgacaaatg ttcctctgct gtttgaccaa gacctgaaaa aatctcttga tacatacggg
     4801 ctacacgagc aggtgtttcc tgcaagccct cgcgattagc gttctctcct acagcctcga
     4861 taatcatttt tacagctgct tcaatctttt gtgtatccat tctcgttctt cctctccatc
     4921 agataggccc tcacttggct caagaaatac aaggaacctg tgataatcct aactgtttgt
     4981 ttctcttctt ttttatctgt caaattctgc tctagaaaat catgccaatc ttggtagctg
     5041 agatttctag ccttagctgc ctctttcagc acgctttcat ccgtcgcccg actatccgca
     5101 aaatgtgtta gagtaagctc ggtatctggc atggctccca gcaagtccaa catatcctcc
     5161 aaggctttgg ttttgataca agtgaagagg atttccttat gatgatccgc aaaacgttct
     5221 tgcaaggtta ccaacaaggc cttgatagca tggggattgt gggctccatc caaaatcatc
     5281 aagggatctc ttgacacgat ttccaaacgc cctggccaac ttgtttcttc caaggcttga
     5341 ccaagaaagt cattgcttgc tagctctcga ccatcttctt gacaaaaagt atcaagtaaa
     5401 gctatggcca tcccagcatt ctctatttgg tacaaaccaa gcaggctagt ctggaagcaa
     5461 ccttgtctga cagcacttgt atagtcaaag acttcccctg tcaccacact ttcttgatga
     5521 cgaacctgat aatctgtccc gtaggcaagt ctcggcgcat ctttctcttc cgcaatgcgg
     5581 tcaatcacag ctaaggcttc tggagcaata cgccctgtta ccaagggcat cccttgtttg
     5641 ataatacctg ccttctgctc tgctatggac tccaaggtgt caccaagtag ggccacatga
     5701 tccaagccaa tagttgtaat tcctgtcaaa atgggctgac agacattggt actatccaaa
     5761 agtccaccca tgccaacttc catgatggcc acatctactt gctctgaggc aaagtagtca
     5821 taggccaggg ctgtgataat ctcaaactcg gttgtgccct gtaaattggc gaccgcttct
     5881 ccctccagca aagactgata gtctgccatg agagcttcta gcctcgcttc tgagatcgat
     5941 tccccattga tgctaatctg gtctgtgtaa tgaatgagat agggcgaact aaacacgcca
     6001 actctcagcc ctagcttttc tagcatcttt ttcaaaaaag caatagtcga gcccttgccg
     6061 ttagtccctc cgatatggag aaccttgagt ttaagatggg gattgccacg caaagctaac
     6121 agttccacca ttcgttccaa gccaaaatgc ggttgatccg tccggtagtt agcaatccac
     6181 tgattgtttt caatttcttt catcttattt atattgtttt aaatctaaat tttccgcttc
     6241 atcagccaga cgaatggcag aggcaatttc aactgccatc ctgtgactag ctacgtcatg
     6301 cacgcgcacc acttctacac cctgtctcgc agcgatacta gttacatgag ccgaagccgt
     6361 gtcccgattt cggaaaccaa gctctgtctc aggattgact tcaaacccac tttcttcaag
     6421 gatattgatg acaaatcgct tgcgcgacac tccgagaaag attggatagc ccttctgatg
     6481 tagtttatcc aggtcccgta aaaggagaag attttctttc ttggtcagac caaagccaat
     6541 tcctggatcc aacaggatat tttctggtgc aataccagct tctgccgctc tcgctagtgc
     6601 tcgttcaaag aaagccacca tcaagtcttc gattggcaat gtttcaaagt cagctaactc
     6661 tttttctgta aaggtttgac caaagccaaa atgagggaag ataagcgaac taggatgctg
     6721 aggtcgagcc ataactgggt taaacatgat gaccactttc gctccagctt tagcaaccac
     6781 atgggccatt ttttcatcac ccatgagacc agtgatatca ttgactagat cggcaccagc
     6841 agccaaagca gcctctgcta cttgactctt ccaagtatca atagagatga ggacatcact
     6901 ttccttgcga atcgctttga tcactggaac aacacgctgg atttcctctt ctatctcaac
     6961 atagctactt cccggccgag tcgattctcc gccgatatcg agcatactgg ctccttctgc
     7021 tatcaattta cgagcctgct ggagcgcctg ctcaagagca aaaaattgac caccgtccga
     7081 aaaggagtct ggggttacat tgataattcc gcaaataact gtctttgcat gattggcttt
     7141 acttgacata tcggtcactc cctcaaggat tttcatcata ttatttttct attttaccat
     7201 aaaaagaaaa agatggacac gattgcattc atctttccct agtagaaaga ggtcggttcc
     7261 tacataaaaa cttttaaaca gaaattccta atgtccgact cataatcacc acaagagcca
     7321 acaaacagaa agcaatccca ttaacaatca tgtgaagcaa gatcgacatt tccaaacgtt
     7381 gggtcttgta ggccgtccaa gatagaactg tcgacatacc tccataaatc aataaagaag
     7441 gtaaattact tggttgatgc aataaagcaa acacaatcgt accgactaca aatcccaagt
     7501 tctccttgcc tcggaaaatc tttttaggaa caatcccacg gcacaagatt tcctcacaaa
     7561 ttggagcaag caaggctagc aagaagaagc tagaaatcag agaactattc tgaaccatat
     7621 cgttaatctg agactggtta gctgttgtcg tctcatttga cagttgcaac aagatagaac
     7681 caagtatatt tgaacagata atgactaaat aactcaagcc caaacgtgcc aaatctttag
     7741 ctctaaaaaa agaaaaatta aaacttgcca attgcgtttt acgagctcca ataataaata
     7801 gcaccagaac cacaatagaa ataccagtaa ctatcagccc tgactgtagc agggctactt
     7861 cttttaaagt caaaatagag gtaaccacta ggggaacctg atataaaagg acggcaagta
     7921 aaaagaccaa aatccagcct gcacgattca acaattcttt ccacatattt ttctctttca
     7981 tcacctatct cctttgtatt aaaaaagggg gaaaatcccc ctgggtcatt ctattataag
     8041 gccatgctga tgtagttaag gataaacaag gcatccaaaa tccaaatcat ggcatgaaca
     8101 tctttaactt gacctttgac aagcttagtc aaagtgtaag tcaagaaacc aactgcaatc
     8161 ccttgagtga tagagtagct gaatcccata aagatagatg tgaagaaggc aggaactgct
     8221 tcagacatat catcccaatg gatatttttc aagctaccaa gcatcataat cccaacgata
     8281 atcaagattg gagctgtagc cgctgttggt acgatcgcta gaagtgggct aaagaagctt
     8341 gaaatcgcaa aacagatagc tacaaccaag gctgtcaaac cagtacgtcc acctgcaccg
     8401 ataccagcag cagactcaac ataagtcgtt acgtttgaag tacctgcaat ggcaccgact
     8461 gtcgttccaa tcaaatcaga gtaaagagcc ttatccaatt tggctgattg gtgattttca
     8521 ccatttgtcg ctacaatacc aactttttca cctgtaccga tcaaggtacc aattgtgtca
     8581 aaaatatctg tcaatgagaa ggcaagaatg gccatcagag tttcaggcaa gcgagctgta
     8641 tctgaaacca aagctcctaa accttctgaa ccaagagctg caccaaagat tgtcttcaaa
     8701 tcttcaaaag ctgcaccaac atggttatta gcaaaatcga tactagacaa atcaaccaaa
     8761 ccaactgcaa tagcaagaac agttgttgtc aagatagaga gaataattcc ccctttaatc
     8821 cctttgatga caaagaagat agtaatggca agtcctgcaa gagccactaa aacagctgga
     8881 ttattaaagc tgaccaatcc tggaactgct gaagagtttg ctgcaatcgt tgcttgagct
     8941 ttgtcagccc cttctcctac aacagtatag ttgcctggat caatcgtgaa tttcaaaagt
     9001 ccagcattct taatccctac ataggcaagg aagacaccga taccagctga aatagctgag
     9061 cgaagagcat tgggaatcga ttcaatgatc attttacgaa catttgtcaa ggtaataatc
     9121 aatgaaataa tcccacagat gaagaccata gctagggctt cttgccaaga ataaccaagc
     9181 ccgaatacaa ctgtaaaggt aaagaaggca ttgagtccca tacctggcgc ttgggcataa
     9241 ggtaagttag cataaaaagc catcatcaag gtacccgcta ctgcaccaat aatcgtcgct
     9301 aggaagacgc cctgagcagg cattcctgtt tgtgaaagta tttgtgggtt tacaaagaga
     9361 atatagctca ttacaaagaa agttgttaaa ccagcgagaa cctctgtacg aacgtctgta
     9421 ccgttctctt ttagtttaaa taatttgtcc atcatcaatc tccttttaat ttttacgaac
     9481 aataatagaa ttatatcatt tatcattcac tttttcaatg tctttgtttg atttgtttaa
     9541 gaaattaatc caatttcatt ttttgtttgg aatctgcttt tctgcctgct ttctgttata
     9601 atagaagaca tcttatctga aaacacacta gaaaggtcca tatgacgaaa aaaattattg
     9661 cagttgacct ggatggaacc ctgctcaact cagacagtca aatttctgac tttaccaaaa
     9721 aaaccatcaa aaaagttgct gaaaaaggcc atcaggttat tattacgaca ggtcgccctt
     9781 accgtatgtc aaaagatttt taccgtgaac tgggcttaga cactcctatg attaacttca
     9841 acggatccct tactcattta ccagaccaag tttgggattt tgaaaagtgt ttgactgtag
     9901 acaaaaaata tctgctagat atggttcaac gttcagagga cattcaagcc gattttatcg
     9961 ctggagaata tcgtaaaaaa ttctacatta caaatcccaa tgaagagatt gccaatccca
    10021 aactatttgg tgtagaagct ttccagcctg aagatcaatt ccagcctgaa ttggtgacca
    10081 aggaccctaa ctgtatcctc ttgcagacca gagccagtga caaatatgcc ttggcaaaag
    10141 acatgaacgc cttctaccag catcaacttt ctatcaatac ctggggaggt ccgctcaata
    10201 tccttgaatg taccccaaaa ggtgtcaaca aggcctttgc tttggactac ttgctcaaga
    10261 taatgaatcg tgacaaaaaa gatttgattg cctttggaga tgaacacaat gataccgaaa
    10321 tgctcgcttt tgctgggaaa ggttatgcca tgaaaaatgc caatccagag ctactccctt
    10381 atgcagatga gcaaatttcc cttaccaacg accaagatgg ggttgccaaa accctacaag
    10441 acttattctt atagcctaca ctgatactca atgagaatca aagagcaaac taggaagcta
    10501 gccgtaggtt gctcaaagca ctgctttgag gttgtagatg aagctgacgt ggtttgaaga
    10561 gattttcgaa gagtatgacc agcttgcaag ctggtctttg tgctcttttc acagtctcat
    10621 caaccagtta atcgattgtt ctactttttg tctccaaaac cttggaaaag gctttctttt
    10681 gtgatatact tcacatataa atacaagaga gctcgtcaca ctcgactctg ttgacaaaaa
    10741 atcaatccag tcccgttgtc cctgttctcg gtcaatatca aggaggatag ctatgaaagc
    10801 tgttgttgta aatccagaaa gcactggtgt tgctattgaa gaaaaagtac tccgtccact
    10861 tgaaactggg gaagcacttg tagaagttga atactgtggc gtttgccaca ccgacctcca
    10921 cgttgctcat ggtgactttg gtcaagtccc aggacgtgtt cttgggcacg aaggtatcgg
    10981 tatcgttaaa gagattgctc cagatgtgaa aagccttaaa gtcggtgacc gcgtcagcgt
    11041 tgcttggttc tttgaaggat gtggcacttg cgaatactgt acaactggtc gcgaaaccct
    11101 ttgccgtaca gtgaaaaatg ctggctactc agtagacggt ggtatggctg aacaatgtat
    11161 cgtaactgct gactatgctg tcaaagttcc tgacggactt gatccagccc aagcttcttc
    11221 tatcacatgt gctggagtaa caacctataa agctatcaaa gaagcaaaag ttgaaccagg
    11281 ccaatgggtt gttctttacg gtgctggtgg acttggtaac ctcgctgttc aatacgctaa
    11341 aaaagtattc aatgctcatg ttatcgcagt cgatatcaac aatgacaaac ttgcccttgc
    11401 aaaagaagta ggcgctgaca ttgtgattaa cggcctcgaa gttgaagatg tagctggact
    11461 catcaaagaa aaaactgatg gaggagctca ttcagctgtc gtaactgctg tgtctaaagt
    11521 tgccttcaac caggctgttg actccgttcg tgctggtggt cgcgtcgtcg ctgttggtct
    11581 tccttctgaa atgatggaac tcagcatcgt taaaacagtc ctcgatggaa tccaagtcat
    11641 cggttctctt gtcggaactc gtaaagactt agaagaagcc ttccaatttg gtgcagaagg
    11701 tctggtagcc ccagttgttc aaaaacgtcc agtagaagat gctgttgcca ttttcgacga
    11761 aatggaaaaa ggtcaaatcc aaggacgtat ggtactcgac ttcacccact aatctaatag
    11821 aaacctattt taaaagttgg aagcttcttc caactttttt atattaaatt t
//
LOCUS       DAVLVP010000054        11753 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.054, whole genome shotgun sequence.
ACCESSION   DAVLVP010000054 DAVLVP010000000
VERSION     DAVLVP010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11753
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.054"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..165)
                     /locus_tag="VYN69_001395"
     CDS             complement(<1..165)
                     /locus_tag="VYN69_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1533652.1"
                     /translation="MEQLHFITKLLDIKDPNIQILDIIKKDTHKEIIAKLDYDAPSCP
                     ECGNQLKKYDF"
     gene            358..1362
                     /locus_tag="VYN69_001396"
     CDS             358..1362
                     /locus_tag="VYN69_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEV1533653.1"
                     /translation="MKNKRLIGIIAALAVLVAGSLIYSSMNKSEAQNNKDEKKITKIG
                     VLQFVSHPSLDLIYKGIQDGLAEEGYKDDQVKIDFMNSEGDQSKVTTMSKQLVANGND
                     LVVGIATPAAQGLASATKDLPVIMAAITDPIGANLVKDLKKPGGNVTGVSDHNPAQQQ
                     VELIKALTPNVKTIGALYSSSEDNSKTQVEEFKAYAEKAGLTVETFAVPSTNEIASTV
                     TVMTSKVDAIWVPIDNTIASGFPTVVSSNQSSKKPIYPSATAMVEVGGLASVVIDQHD
                     LGVATGKMIVQVLKGAKPADTPVNVFSIGKSVINKKIAQELGITIPESVLKEAGQVIE
                     "
     gene            1431..2297
                     /locus_tag="VYN69_001397"
     CDS             1431..2297
                     /locus_tag="VYN69_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1533654.1"
                     /translation="MIVSIISQGFVWAILGLGIFMTFRILNFPDMTTEGSFPLGGAVA
                     VTLITKGVNPFLATLVAVGAGCLAGMAAGLLYTKGKIPTLLSGILVMTSCHSIMLLIM
                     GRANLGLLGTKQIQDVLPFDSDLNQLLTGLIFVSIVIALMLFFLDTKLGQAYIATGDN
                     PDMARSFGIHTGRMELMGLVLSNGVIALAGALIAQQEGYADVSRGIGVIVVGLASLII
                     GEVIFKSLSLAERLVTIVVGSIAYQFLVWAVIALGFNTSYLRLYSALILAVCLMIPTF
                     KQTILKGAKLSK"
     gene            2294..3052
                     /locus_tag="VYN69_001398"
     CDS             2294..3052
                     /locus_tag="VYN69_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533655.1"
                     /translation="MTAIVELKNATKIVKNGFDEEKIILNDVSLEIFEQDFITILGGN
                     GAGKSTLFNTIAGTISLTSGTIRILGEDVTKFSPEKRAKYLSRVFQDPKMGTAPRMTV
                     AENLLIAKFRGEKRGLFPRRLASYKDKFQATIEKVGNGLEKHLNTPIEFLSGGQRQAL
                     SLLMATLKRPELLLLDEHTAALDPKTSVALMELTDEFVKKDQLTALMITHHMEDALKY
                     GNRLIVMKEGRVIQDLNQEEKAKMKISDYYQLFE"
     gene            3142..4902
                     /gene="dnaG"
                     /locus_tag="VYN69_001399"
     CDS             3142..4902
                     /gene="dnaG"
                     /locus_tag="VYN69_001399"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000227865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEV1533656.1"
                     /translation="MVDKQVIEEIKNNANIVEVIGDVISLQKVGRNYLGLCPFHGEKT
                     PSFNVVEDKQFYHCFGCGRSGDVFKFIEEYQGVPFIEAVQILGQRVGIEVEKPLYSEQ
                     KPASPHQALYDMHEDAAKFYHAILMTTTMGEEARNYLYQRGLTDEVLKHFWIGLAPPE
                     RNYLYQRLSDQYREEDLLDSGLFYLSDANQFVDTFHNRIMFPLTNDQGKVIAFSGRIW
                     QKTDSQTSKYKNSRSTAIFNKSYELYHMDRAKKSSGKTSEIYLMEGFMDVIAAYRAGI
                     ENAVASMGTALSREHVEHLKRLTKKLVLVYDGDKAGQAATLKVLDEIGDMPVQIVSMP
                     DNLDPDEYLQKNGPEDLAYLLTKTRINPIEFYIHQYKPENSENLQAQIEFIEKIAPLI
                     VQEKSIAAQNSYIHILADSLVSFDYAQIEQIVNESRQVQRQNRMEGISRPTPITMPIT
                     KQLSAIMRAEAHLLYRMMESPLVLNDYRLREDFAFATPEFQVLYDLLGQYGNLPPEVL
                     AEQTEEVERAWYQVLAQDLPAEISPQELSEVEMTRNKALLNQDNMRIKKKVQEASHVG
                     DTDTALEELERLISQKRRME"
     gene            4905..6014
                     /gene="rpoD"
                     /locus_tag="VYN69_001400"
     CDS             4905..6014
                     /gene="rpoD"
                     /locus_tag="VYN69_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEV1533657.1"
                     /translation="MATKQKEVTTFDVQVAEFIRNHKQKGTATDDEINASLVIPFTLD
                     ADGIEDLLQRIQDAGISITDNEGNPSARVLSNEEEPELSDEDLIGSTSAKVNDPVRMY
                     LKEIGVVPLLTNEEEKELALAVEAGDIEAKQRLAEANLRLVVSIAKRYVGRGMQFLDL
                     IQEGNMGLMKAVDKFDYSKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LVREQRNLLQELGQDPTPEQIAERMDMTPDKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDEVIENPVDYTTRIVLREQLDEILDTLTDREENVLRLRFGLDDGKMRTLEDVGK
                     VFNVTRERIRQIEAKALRKLRQPSRSKPLRDFIED"
     gene            6028..6357
                     /locus_tag="VYN69_001401"
     CDS             6028..6357
                     /locus_tag="VYN69_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEV1533658.1"
                     /translation="MAYTEEQIENIKTRILTALEEVIDPELGIDIVNLGLIYEIRFDG
                     DTGQTEIDMTLTTMGCPLADLLTDQIYDAMIEVPEVTDTEVKLVWYPAWTVEKMSRYA
                     RIALGIK"
     gene            6391..7515
                     /gene="cpoA"
                     /locus_tag="VYN69_001402"
     CDS             6391..7515
                     /gene="cpoA"
                     /locus_tag="VYN69_001402"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010976517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosylglucosyldiacylglycerol synthase"
                     /protein_id="HEV1533659.1"
                     /translation="MRKFPLFSSSLSFLLLILFKENDIIVVMEKKKLRINMLSSSEKV
                     AGQGVSGAYRELVRLLHRAAKDQLIVTENLPIEADVTHFHTIDFPYYLSTFQKKRSGR
                     KIGYVHFLPATLEGSLKIPFFLKGIVKRYVFSFYNRMEHLVVVNPMFIEDLVAAGIPR
                     EKVTYIPNFVNKEKWHPLPQEEVVRLRTDLGLSDNQFIVVGAGQVQKRKGIDDFIRLA
                     EELPQITFIWAGGFSFGGMTDGYEHYKKIMENPPKNLIFPGIVSPERMRELYALADLF
                     LLPSYNELFPMTILEAASCEAPIMLRDLDLYKVILEGNYRATAGREEMKEAILEYQAN
                     PAVLKDLKEKAKNISREYSEEHLLQIWLDFYEKQAALGRK"
     gene            7531..8856
                     /locus_tag="VYN69_001403"
     CDS             7531..8856
                     /locus_tag="VYN69_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEV1533660.1"
                     /translation="MRIGLFTDTYFPQVSGVATSIRTLKTELEKQGHAVFIFTTTDKD
                     VNRYEDWQIIRIPSVPFFAFKDRRFAYRGFSKALEIAKQYQLDIIHTQTEFSLGLLGI
                     WIARELKIPVIHTYHTQYEDYVHYIAKGMLIRPSMVKYLVRGFLHDVDGVICPSEIVR
                     DLLSDYKVKVEKRVIPTGIELAKFERPEIKQENLKELRSKLGIQDGEKTLLSLSRISY
                     EKNIQAVLVAFADVLKEEDKVKLVVAGDGPYLNDLKEQAQNLEIQDSVIFTGMIAPSE
                     TALYYKAADFFISASTSETQGLTYLESLASGTPVIAHGNPYLNNLISDKMFGTLYYGE
                     HDLAGAILEALIATPDMNEHTLSEKLYEISAENFGKRVHEFYLDAIISNNFQKDLAKD
                     DTVSQRIFKTVLYLPQQVVAVPVKGSRRMLKASKTQLISMRDYWKDHEE"
     gene            8887..9063
                     /locus_tag="VYN69_001404"
     CDS             8887..9063
                     /locus_tag="VYN69_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEV1533661.1"
                     /translation="MRSGRDKKIAGVCAGVAHYLDMDPTIVQVIWGVLTCCYGAGIVA
                     YIILWIIAPVATDY"
     gene            9122..9256
                     /locus_tag="VYN69_001405"
     CDS             9122..9256
                     /locus_tag="VYN69_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000863859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEV1533662.1"
                     /translation="MAFGDNGNRKKTMFEKITLFIVIIMLVASLLGIFATAIGALSNL
                     "
     gene            9320..10630
                     /gene="obgE"
                     /locus_tag="VYN69_001406"
     CDS             9320..10630
                     /gene="obgE"
                     /locus_tag="VYN69_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000061643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEV1533663.1"
                     /translation="MSMFLDTAKIKVKAGNGGDGMVAFRREKYVPNGGPWGGDGGRGG
                     NVVFVVDEGLRTLMDFRYNRHFKADSGEKGMTKGMHGRGAEDLRVRVPQGTTVRDAET
                     GKVLTDLIEHGQEFIVAHGGRGGRGNIRFATPKNPAPEISENGEPGQERELQLELKIL
                     ADVGLVGFPSVGKSTLLSVITSAKPKIGAYHFTTIVPNLGMVRTQSGESFAVADLPGL
                     IEGASQGVGLGTQFLRHIERTRVILHIIDMSASEGRDPYEDYLAINKELESYNLRLME
                     RPQIIVANKMDMPESQENLEEFKKKLSENYDEFEELPAIFPISGLTKQGLATLLDATA
                     ELLDKTPEFLLYDESDMEEEAYYGFDEEEKAFEISRDDDATWVLSGEKLMKLFNMTNF
                     DRDESVMKFARQLRGMGVDEALRARGAKDGDLVRIGKFEFEFVD"
     gene            10640..10798
                     /locus_tag="VYN69_001407"
     CDS             10640..10798
                     /locus_tag="VYN69_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533664.1"
                     /translation="MGDKPISFRDADGNFVSAADVWNEKKLEELFNRLNPNRALRLAR
                     TKKENPSQ"
     gene            complement(10850..11753)
                     /locus_tag="VYN69_001408"
                     /pseudo
     CDS             complement(10850..11753)
                     /locus_tag="VYN69_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436393.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         3562 a         2067 c         2710 g         3414 t
ORIGIN      
        1 aaagtcatat ttcttcaatt ggtttccgca ctcagggcaa gatggggcgt cgtagtccag
       61 tttggcgatg atttccttgt gtgtatcctt cttgatgatg tctaaaatct ggatattagg
      121 gtctttaatg tctagtaatt ttgtgataaa atgtaattgt tccatatgaa tctttctaat
      181 gagttgtttt gtcgcttttc attataggtc atatgggact ttttttctac aataaaatag
      241 gctccataat atctatagtg gatttaccca ctacaaatat tatagaacca agaaaacgat
      301 ggcgtcgctt gtttagataa gtgaaatatg ttaaaggaaa taaaaaggag aaacagaatg
      361 aaaaataaac gtttaattgg aattattgct gcattagcag tcttagtagc aggaagcttg
      421 atttattctt caatgaataa atcagaagct cagaataata aggatgagaa gaaaataacc
      481 aagattggtg tgcttcaatt tgtgagccat ccatcccttg atttgattta taaagggatc
      541 caagatggac ttgcagaaga aggatataaa gatgatcaag ttaaaattga ttttatgaac
      601 tcagaaggtg accaaagtaa ggttacgaca atgagtaaac aattggttgc aaatggaaat
      661 gaccttgtgg ttggtatcgc aacaccagca gcccaagggt tggctagtgc aacaaaagac
      721 ctaccggtta tcatggccgc tattacagac ccaattggtg ctaacttggt taaagatttg
      781 aaaaaaccag gtggcaacgt tacaggggta tctgaccaca atccagctca acaacaagtt
      841 gaactcatca aggctctgac accgaatgtg aaaacaatcg gagctcttta ctcaagtagc
      901 gaagacaatt caaaaacaca ggtcgaagaa tttaaggctt atgctgaaaa agcaggtctg
      961 acagtggaaa catttgcagt tccttcaaca aatgaaattg cctcaactgt cactgttatg
     1021 actagcaagg tagatgctat ttgggttcca attgataaca ccattgcatc aggatttcca
     1081 acggttgtct ctagcaatca aagttctaag aaaccaattt atcccagtgc gacagctatg
     1141 gtagaagtag gtggtttggc atcagttgta attgaccaac atgaccttgg tgtggcaaca
     1201 ggtaaaatga ttgtgcaagt cttgaaaggt gcaaaaccag ccgatacccc agtcaatgtc
     1261 ttttcaattg gtaagtcagt catcaataaa aaaatagcac aagaactagg tattactatt
     1321 cctgagtctg ttctcaaaga agcaggacaa gtcatcgaat aaataaggta gggaggagga
     1381 gactcttccc attttttaaa aagatgaata tagaaaggat taaataaaat atgattgttt
     1441 ccattatttc tcaaggattt gtctgggcta ttctaggtct gggaatcttt atgacattta
     1501 ggattttaaa ctttccagat atgacgacag aaggttcctt ccctcttggg ggagctgttg
     1561 ctgtcacttt gataaccaaa ggcgtgaacc catttttagc gacacttgtt gctgtaggag
     1621 caggttgttt ggctggaatg gcagcaggcc ttctttatac aaaagggaag atcccaacct
     1681 tgctctcagg gattttggtg atgacttctt gtcactcaat catgctcttg attatgggac
     1741 gtgcgaattt aggcctgctt ggaaccaagc aaattcagga tgttttgcct tttgattcgg
     1801 atttgaatca actcttgaca ggtctcatct ttgtgagtat tgttattgct ctcatgctct
     1861 ttttcttgga cactaaactc ggacaagcct atattgctac aggggataat cctgatatgg
     1921 ctagaagttt cgggattcat actggacgca tggagctcat gggcttggtc ttatcaaatg
     1981 gtgtgattgc ccttgcaggt gccctcattg ctcagcaaga aggttatgcc gatgtgtctc
     2041 gagggatcgg ggttatcgtt gtggggcttg caagtttgat tattggagaa gttattttca
     2101 agagtttgag cttggcagag cgtttggtta ctatcgttgt aggttctatc gcttatcaat
     2161 ttttagtgtg ggcagttatc gcacttggct ttaatacaag ttaccttcgt ttatacagtg
     2221 ccttgatttt agcagtctgc ctcatgattc caacatttaa gcaaacaatc ttgaaaggag
     2281 ccaagttaag caaatgacag caattgtaga attaaaaaat gcaaccaaaa tcgttaaaaa
     2341 tggctttgat gaagaaaaga ttattttaaa tgatgtttcc ttagaaattt ttgaacagga
     2401 ctttatcacg attttgggcg gaaatggtgc tggtaaatca accctcttta acactattgc
     2461 aggaaccata tcactaacca gtggaactat ccgtatttta ggtgaggatg ttaccaagtt
     2521 ttcaccagag aagcgcgcta agtatctgtc tcgagtcttc caagatccaa agatggggac
     2581 agctcctcgt atgacagtgg cagagaatct cctgattgcc aagtttcgtg gtgaaaagcg
     2641 tggactattt ccgagacgtc tggctagcta taaggataag tttcaggcga ccattgaaaa
     2701 agtgggaaac ggtcttgaga aacacttgaa tacaccgatt gagttcttat caggtggaca
     2761 aagacaggct ttgagtctct tgatggcaac cttgaagcga cctgaattac tcttgttaga
     2821 cgagcatact gctgccctgg atccaaagac tagtgttgct ttgatggaat tgacagatga
     2881 atttgttaag aaagatcaat tgacagccct tatgattacc catcatatgg aagatgctct
     2941 caaatatggc aatcgcttga ttgtcatgaa agaaggacgg gttattcaag atttaaacca
     3001 agaagaaaaa gcaaaaatga aaatctctga ttattatcaa ctctttgaat aaagtaggga
     3061 aaatgagtga aatggtttac tttttttctg aaataaagta tactatataa agtaaactat
     3121 gataacatgg aggtattgtg tatggttgac aaacaagtca ttgaagaaat caaaaacaat
     3181 gccaacattg tggaagtcat aggagatgtg atttctttac aaaaggtagg acggaactat
     3241 ctagggctct gtccttttca tggtgaaaaa acaccttctt tcaacgttgt agaggataag
     3301 cagttttatc actgtttcgg ttgtggtcgc tcaggtgatg tttttaagtt catcgaggag
     3361 taccaagggg ttccctttat agaggcagtc caaatcttag gtcagcgtgt cgggattgag
     3421 gttgaaaaac cgctttatag tgaacagaag ccagcctcgc ctcaccaagc tctttatgat
     3481 atgcacgaag atgcggctaa attttaccat gctattctca tgacaacgac tatgggcgaa
     3541 gaggccagaa attaccttta tcagcggggt ttgacagatg aagtgcttaa acatttttgg
     3601 attggtttag cacctccaga acgaaactat ctctatcaac gtttgtctga tcagtatcgt
     3661 gaagaagatt tactggattc aggcctgttt tatctctcgg atgccaatca atttgtagac
     3721 acctttcaca atcgcattat gtttcccctg acaaatgacc aaggaaaggt cattgccttc
     3781 tcaggtcgta tctggcaaaa aacagattca caaacttcta agtataaaaa cagccgatcg
     3841 actgcaattt ttaacaaaag ttacgaatta tatcatatgg atagggcaaa aaaatcttct
     3901 ggaaaaacta gtgagattta cctgatggaa ggattcatgg atgttattgc agcctatcgg
     3961 gctggaatcg aaaatgctgt ggcgtcgatg ggaacggcct tgagtcgaga gcatgttgag
     4021 catctgaaaa ggttaaccaa gaaattggtt cttgtttacg atggagataa ggctgggcaa
     4081 gccgcgacat tgaaagtatt ggatgaaatt ggtgatatgc ctgtgcaaat cgtcagcatg
     4141 cctgataact tggatcctga tgagtatcta caaaaaaatg gtccagaaga cttggcctat
     4201 ctattaacga aaactcgtat taatccgatt gagttctaca ttcatcagta caaacctgaa
     4261 aacagtgaaa atctgcaggc tcagattgag tttattgaaa aaatagctcc cttgattgtt
     4321 caagaaaagt ccatcgctgc tcaaaacagc tatattcata ttttagctga cagtctggtg
     4381 tcctttgatt atgcccagat tgagcagatt gttaatgaga gtcgtcaggt gcaaaggcag
     4441 aatcgcatgg aaggaatttc cagaccgacg ccaatcacca tgcctatcac caagcagtta
     4501 tcggctatta tgagggcaga agcccatcta ctctatcgga tgatggaatc ccctcttgtt
     4561 ttgaacgatt accgtttgcg agaagacttt gcatttgcta cacctgaatt tcaggtctta
     4621 tatgacttgc ttggccagta tggaaatctt cctccagaag ttttagcaga gcagacagag
     4681 gaagttgaaa gagcttggta ccaagtttta gctcaggatt tgcctgctga gatatcgccg
     4741 caggaactta gtgaagtaga gatgactcga aacaaggctc tcttgaatca ggacaatatg
     4801 agaatcaaaa agaaggtgca ggaagctagc catgtaggag atacagatac agccctagaa
     4861 gaattggaac gtttaatttc ccaaaagaga agaatggagt aataatggca acaaaacaaa
     4921 aagaagtaac aacatttgac gtacaggtag cagaatttat ccgtaatcat aagcaaaaag
     4981 ggacagcaac agatgatgaa atcaatgcaa gtctggttat tccttttacc ttggacgctg
     5041 atgggattga agatctcttg caacggattc aggatgcagg gatttctatc acagataacg
     5101 aaggaaatcc aagtgcgcgt gttcttagca atgaagaaga accagaactc agcgatgagg
     5161 acttgattgg gtcaacttct gctaaggtca atgaccctgt ccgtatgtac ttgaaagaaa
     5221 taggggtcgt tcctctcttg accaatgaag aggagaaaga gttggcactg gctgttgaag
     5281 ctggtgatat cgaagccaaa caacgtcttg cggaagccaa tcttcgtttg gttgtttcca
     5341 ttgccaaacg ctatgtcggt cgtggtatgc agttccttga cttgattcaa gaaggaaata
     5401 tgggcttgat gaaggcggtt gacaagtttg actattctaa agggttcaag ttttcaactt
     5461 atgcaacttg gtggattcgt caggctatca ctcgtgctat tgcggaccaa gctcgtacca
     5521 tccgtatccc agttcacatg gttgaaacta tcaataaatt ggttcgtgaa cagcggaatc
     5581 tccttcaaga attggggcaa gatccgacac cagaacagat tgctgaacga atggatatga
     5641 cacctgataa ggttcgtgaa atcttgaaga ttgcccaaga accagtatct cttgaaactc
     5701 ctatcggtga agaggacgat agccaccttg gagactttat cgaagatgaa gtgattgaaa
     5761 atccagtgga ttatacgact cgtatcgtct tgcgtgagca attggatgaa atcttagata
     5821 ctcttacaga ccgtgaagaa aatgttctgc gtctacgttt tggactagat gatggaaaaa
     5881 tgcgcacact tgaagatgtg gggaaagtct ttaacgtaac tcgtgagcgt atccgtcaga
     5941 ttgaagcaaa ggctttgaga aaactacgcc aaccaagtcg tagcaaaccg cttcgtgatt
     6001 ttattgaaga ctaagagtga ggaaaatatg gcttatacag aagagcaaat tgaaaacatc
     6061 aaaacacgga ttttaacagc cttggaagaa gtcatcgacc ctgagttggg aatcgatatt
     6121 gtcaatcttg gtttgatcta tgagattcgt tttgacggtg acacagggca aacagagatt
     6181 gacatgactt tgacaactat gggttgtccc ttagcagacc ttttgacaga ccagatttat
     6241 gatgctatga tagaggttcc agaagtaacg gatactgagg ttaaattagt ctggtatcca
     6301 gcctggacag ttgaaaaaat gagtcgctat gcccgcattg ccctaggcat taagtaaaca
     6361 tataaaagca tgtgagagac tgttggaaaa gtgaggaaat ttcccctctt ttcctctagt
     6421 ctctcctttc ttttgctgat tttattcaaa gaaaatgata taatagtagt tatggagaaa
     6481 aagaaattac gcatcaatat gttgagttca agtgagaaag tagcaggaca gggagtttca
     6541 ggtgcttacc gtgaattagt tcgtcttctt caccgtgctg ccaaggacca attgattgtt
     6601 acagaaaatc ttccaatcga ggcagatgtg actcactttc atacgattga ttttccctat
     6661 tatttatcaa ccttccaaaa gaaacgctca gggagaaaga ttggctatgt gcatttcttg
     6721 ccagctacac ttgagggaag tttgaaaatt ccatttttct taaagggaat tgtgaaacgc
     6781 tatgtatttt ctttttacaa ccggatggag cacttggttg tggtcaatcc tatgtttatt
     6841 gaggatttgg tagcagctgg tattccacgt gaaaaagtga cctatattcc taactttgtc
     6901 aacaaggaaa aatggcatcc tctaccacaa gaagaggtag ttagactgcg cacagatctt
     6961 ggtcttagtg acaatcagtt tatcgtagta ggtgctgggc aagttcagaa acgtaaaggg
     7021 attgatgact ttatccgtct ggctgaggaa ttgcctcaga ttacctttat ctgggctggt
     7081 ggcttctctt ttggtggtat gacagatggt tatgaacact ataagaaaat tatggaaaat
     7141 ccccctaaaa atttgatttt tccaggcatt gtatcgccag agcggatgcg cgaattgtat
     7201 gctctagcgg atcttttctt gttgcctagt tacaatgagc tctttcctat gactatttta
     7261 gaagctgcga gttgtgaggc tcctattatg ttgcgtgatt tagatctcta taaggtgatt
     7321 ttggagggaa attatcgggc gacagcgggt agagaagaga tgaaagaggc tattttggaa
     7381 tatcaagcaa atcctgctgt cttaaaagat ctcaaagaaa aggctaagaa tatttccaga
     7441 gagtattctg aagagcatct gttacaaatc tggttggact tttatgagaa acaagccgct
     7501 ttagggagaa agtaaaaagt gaggtaatct atgcgaattg gtttatttac agatacctat
     7561 tttcctcagg tttctggtgt tgcgaccagt attcgaacct tgaaaacaga acttgaaaag
     7621 cagggacatg ctgtttttat ctttacgacg acagataagg atgtcaatcg ctacgaagat
     7681 tggcaaatta tccgcattcc aagtgttcct ttctttgctt ttaaggatcg tcgctttgcc
     7741 taccgaggtt ttagcaaggc acttgaaatt gctaaacagt atcagctaga tattatccat
     7801 actcagacag aattttctct tggcctgttg gggatttgga ttgcgcgtga attgaaaatt
     7861 ccagtcatcc atacctatca cacccagtat gaagactatg tccattatat tgctaagggg
     7921 atgttgatcc ggccgagtat ggtcaagtat ctggttagag gtttcctgca tgatgtggat
     7981 ggggttattt gccctagtga gattgtccgt gacttgctat ctgattataa ggtcaaggtt
     8041 gaaaaacggg tcattcctac tgggattgaa ttagccaagt ttgagcgtcc ggaaatcaag
     8101 caggaaaatt tgaaagaact gcgtagtaaa ctagggattc aagatggtga aaagacgttg
     8161 cttagtcttt cgagaatctc ctatgaaaaa aatattcaag cagttttagt agcctttgct
     8221 gatgttctga aagaggaaga caaggttaaa ctggtagtag ctggggatgg cccttatctg
     8281 aatgacctca aagagcaagc ccagaaccta gagattcaag actcagtcat ctttacaggg
     8341 atgattgctc ctagtgagac ggctctttac tataaagcgg cggatttctt catttcggca
     8401 tcgacaagcg aaacgcaagg tttgacctac ttggaaagct tagccagtgg aacacctgtc
     8461 attgctcacg gaaatcctta tttgaacaac ctcatcagtg ataaaatgtt tggaaccttg
     8521 tactatggag aacatgattt ggctggtgct attttggaag ccctgattgc aacaccagac
     8581 atgaacgagc ataccttatc agagaaattg tatgagattt cagctgagaa ctttgggaaa
     8641 cgagtgcatg agttttatct ggatgccatt atttcaaata acttccagaa agatttggct
     8701 aaagatgata cggtcagtca gcgtatcttt aagacagttt tgtatcttcc gcaacaggtg
     8761 gttgctgtac ctgtaaaagg atctagacgc atgttgaagg cttcaaaaac acagttgatc
     8821 agtatgagag actattggaa agaccatgaa gaatagaaag aggaacagct atgaaaaaaa
     8881 caattaatga gaagcggtcg tgataaaaag attgcgggtg tttgtgctgg ggtggcccat
     8941 tatctggata tggatccgac tatcgttcaa gtcatttggg gtgttcttac ttgctgttac
     9001 ggagctggaa ttgtagctta cattatttta tggattatcg caccagtagc aactgactat
     9061 tgaaaactag ctcaattcat gctaagataa tagaaaatag aatgtaacga aggagagaaa
     9121 aatggcattt ggagataatg gaaatcgtaa aaaaactatg tttgagaaaa taaccttgtt
     9181 tatcgtgatt atcatgctag tagcaagttt attgggaatt tttgcaactg caattggtgc
     9241 cctcagtaat ctataaaata gattcaagaa aatttagtga ctgggatttc ccagcccttt
     9301 tttaaagtga gaagaaataa tgagtatgtt tttagataca gctaagatta aggtcaaggc
     9361 tggtaatggt ggcgatggta tggttgcctt tcgtcgtgaa aaatatgtcc ctaatggagg
     9421 cccttggggt ggtgatggtg gtcgtggagg caatgtggtc ttcgttgtag acgaaggact
     9481 acgtaccttg atggatttcc gctacaatcg tcatttcaag gctgattctg gtgaaaaagg
     9541 gatgaccaaa gggatgcatg gtcgtggtgc tgaggacctt agagttcgag taccgcaagg
     9601 tacgactgtt cgtgatgcgg agactggcaa ggttttaaca gatttgattg aacatgggca
     9661 agaatttatc gttgcccacg gtggtcgtgg tggacgtgga aatattcgtt tcgcgacacc
     9721 aaaaaatcct gcaccggaaa tctctgaaaa tggagaacca ggtcaggaac gtgagttaca
     9781 attggaacta aaaatcttgg cagatgtcgg tttagtagga ttcccatctg tagggaagtc
     9841 aacactttta agtgttatta cctcagctaa gcctaaaatt ggtgcctacc actttaccac
     9901 tattgtacca aatttaggta tggttcgcac ccaatcaggt gaatcctttg cagtagccga
     9961 cttgccaggt ttgattgaag gggctagtca aggtgttggt ttgggaactc agtttctccg
    10021 tcacatcgag cgtacacgtg ttatccttca catcattgat atgtcagcta gcgaaggccg
    10081 tgatccatat gaggattacc tagctatcaa taaagagctg gagtcttaca atcttcgcct
    10141 catggagcgt ccacagatta ttgtagctaa taagatggac atgcctgaga gtcaggaaaa
    10201 tcttgaagaa tttaagaaaa aattgtctga aaattatgat gaatttgaag agttaccagc
    10261 tatcttccca atttctggat tgaccaagca aggtctggca acacttttag atgctacagc
    10321 tgaattgtta gacaagacac cagaattttt gctctacgac gagtccgata tggaagaaga
    10381 agcttactat ggatttgacg aagaagaaaa agcctttgaa attagtcgtg atgacgatgc
    10441 gacatgggta ctttctggtg aaaaactcat gaaactcttt aatatgacca actttgatcg
    10501 tgatgaatct gtcatgaaat ttgcccgtca gcttcgtggt atgggggttg atgaagccct
    10561 tcgtgcgcgt ggagctaaag atggggattt ggtccgcatt ggtaaatttg agtttgaatt
    10621 tgtagactag gagactggta tgggagataa accgatatct ttccgagatg cggatggtaa
    10681 ttttgtttcc gccgcagacg tttggaatga aaagaaattg gaagaactat ttaatcgtct
    10741 caatccaaat cgtgccttga gattggcacg aactaaaaag gaaaatccat ctcagtaaag
    10801 aagctaaaaa atcccggctc tttgtcaact gtagtgggtt gaaaaaaagc taagcttgag
    10861 aaaggacaaa tttcgtcctt tcttttttga tattcagggc gataaaaatc ctttttttga
    10921 agttttcaaa gtttcgaaaa ccaaaggcat tgcgcttgat aagtttgatg agattattgg
    10981 tcgcttccaa tttggcgttt gaatagggta gttgaagggc gttgacgatt ttctctttgt
    11041 cctttagaaa ggttttaaag acagtctgaa aaataggatg aacctgctta agattgtcct
    11101 caataagtcc gaaaaatttc tctggttcct tattctggaa gtgaaacagc aagagttgat
    11161 agagctgata gtgatgtttc aagtcttgtg aatagctcaa aagcttgttt aaaatctctt
    11221 tattggttaa gtgcatgcga aaagtagggc gataaaaacg tttatcgctc aatttacggc
    11281 tatcctgttg aatgagtttc cagtagcgct tgatagcctt gtattcatgg gattttcgat
    11341 gaaactgatt catgatttgg acacgcacac gactcatagc acggctaaga tgttgtacaa
    11401 tgtgaaagcg atcaagaacg attttagcat tcgggagtga aacagcctgg gagactgttt
    11461 cagcctgagc ctagaaattt gaaagcgaag ctgtttagcc aagtcatagt aagggctaaa
    11521 catatccata gtaatgattt tgacgcgaca tcggacggct ctatcatatt taagaaagtg
    11581 atctcggatg acagcttgtg ttctgccttc aagaacagtg ataatattaa gattatcaaa
    11641 atcttgcgca atgaagctca tctccatctc ccgattgaaa cagtcactcc ccggactgtt
    11701 tcaacctccc aagacataat ctcaggcaga tttctaaaat tacactcaaa gtg
//
LOCUS       DAVLVP010000055        11532 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.055, whole genome shotgun sequence.
ACCESSION   DAVLVP010000055 DAVLVP010000000
VERSION     DAVLVP010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.055"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            112..765
                     /locus_tag="VYN69_001409"
     CDS             112..765
                     /locus_tag="VYN69_001409"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEV1533665.1"
                     /translation="MEHSSWHALIKAQLPEGYFGKINQFMEQVYSQGIIYPPKEKVFQ
                     ALLTTLLEEVKVVILGQDPYHGPGQAQGLSFSVPDSIPAPPSLQNILKELSDDIGVKK
                     SHDLTAWAEQGVLLLNACLTVPAGQANGHAGQIWEPFTDAVIQVVNHLDRPVVFVLWG
                     AYARKKKALVTNPHHLIIESAHPSPLSVYRGFWGSKPFSKANTFLKETGQEPIDWLR"
     gene            775..1239
                     /locus_tag="VYN69_001410"
     CDS             775..1239
                     /locus_tag="VYN69_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003012002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1533666.1"
                     /translation="MPQLATICYIDNGKELLMLHRNKKPNDVHEGKWIGVGGKLERGE
                     TPQECAVREILEETGLKAKPVLKGVITFPEFTPELDWYTYVFKVTEFEGDLIDCNEGM
                     LEWVPYDEVLSKPTWEGDHTFVEWLLEDKPFFSAKFVYDGDKLLDTQVDFYE"
     gene            1252..2520
                     /locus_tag="VYN69_001411"
     CDS             1252..2520
                     /locus_tag="VYN69_001411"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000924471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEV1533667.1"
                     /translation="MLLIKNGRVMDPKSGLDQVCDVLVQDGKIIKIAPEITEEGAETI
                     DATGHVVAPGLVDIHVHFREPGQTHKEDIHTGALAAAAGGFTTVVMMANTSPTISDVE
                     TLQAVLQSAAKEKINVKTVATITKNFNGKNLTDFKALLEAGAVGFSDDGIPLESSKIV
                     KEAMEEAKKLNTFISLHEEDPGLNGVLGFNENIAREHFHICGATGVAEYAMMARDVMI
                     AYATKAHVHIQHLSKEESVKVVEFAQGLGAEVTAEVAPQHFSKTEALLLTQGSNAKMN
                     PPLRLESDRRAVIEGLKSGVITVIATDHAPHHVDEKNVEDITKAPSGMTGLETSLSLG
                     LTYLVEAGELSLMELLEKMTYNPAKLYNFKAGYLAENGPADITIFDAKADRLVDSHFA
                     SKAANSPFIGETLKGQVKYTICKGQIVYQA"
     gene            2534..2794
                     /locus_tag="VYN69_001412"
     CDS             2534..2794
                     /locus_tag="VYN69_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533668.1"
                     /translation="MNYFRKRRERQAKSNSGIYCPAANRSKIQYETKEKADHAVQYDN
                     GGLVRSYYCRTCACWHTTSKSNKPPLSLKNYGLKLFGLDKSE"
     gene            2805..4166
                     /gene="pdrM"
                     /locus_tag="VYN69_001413"
     CDS             2805..4166
                     /gene="pdrM"
                     /locus_tag="VYN69_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-coupled multidrug efflux MATE transporter
                     PdrM"
                     /protein_id="HEV1533669.1"
                     /translation="MYKTKCLREKLVLFLKIFFPILIYQFANYSASFVDTAMTGQYNT
                     MDLAGVSMATSIWNPFFTFLTGIVSALVPIIGHHLGRGKKEEVASDFYQFIYLALGLS
                     VVLLGMVLFLAPTILNHIGLEAAVAAVAVRYLWFLSIGIIPLLLFSVIRSLLDSLGLT
                     KLSMYLMLLLLPLNSGFNYLLIYGAFGVPELGGAGAGLGTSLAYWVLLGISVLVLFKQ
                     EKLKALHLEKRIPLNMDNIKEGVRLGLPIGGTVFAEVAIFSVVGLIMAKFSSLIIASH
                     QSAMNFSSLMYAFPMSISSAMAIVVSYEVGAKRFDDAKTYIGLGRWTALIFAAFTLTF
                     LYIFRGNVASLYGNDPKFIDLTARFLTYSLFFQLADTFAAPLQGILRGYKDTVIPFYL
                     GLLGYWGVAIPVATLFDSLTDFGAYSYWIGLIISLIVSGALYRWRLTVIMKRFESLAK
                     SKR"
     gene            4320..5288
                     /locus_tag="VYN69_001414"
     CDS             4320..5288
                     /locus_tag="VYN69_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HEV1533670.1"
                     /translation="MSTLDKNLLLEMFRKMEEIRRMDLKIAQLVKKGKVPGMTHFSVG
                     EEAANVGAMLALNPDDLITSNHRGHGQAIAKGIDLNGMMAEILGKYTGTCKGKGGSMH
                     IADLDAGNLGANGIVGGGMGIAVGAALSQQMQNTGKIVVCFFGDGATNEGVFHEAVNM
                     ASIWNLPVIFYCINNGYGISADIKKMTNIEHIHQRSAAYGIPGMFIEDGNNVIDVYEG
                     FQKAVDHVRSGNGPVLIESVTYRWLGHSSSDPGKYRTREEVELWKQKDPIENLRNYLI
                     ENNIASAEELEEIQAQVKEAVEASVKFAEESPFPPLESAFEDIYAD"
     gene            5304..6296
                     /locus_tag="VYN69_001415"
     CDS             5304..6296
                     /locus_tag="VYN69_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEV1533671.1"
                     /translation="METKTMSFRDTIILAMSEEMRRDENVFLMGEDVGVFGGDFGTSV
                     GMLEEFGPERVRDCPISEAAISGAAAGAAMTGLRPIVDMTFMDFSVIAMDNIVNQAAK
                     TRYMFGGKGQVPMTVRCAAGNGVGSAAQHSQSLESWFTHIPGLKVVAPGTPADMKGLL
                     KSSIRDKNPVIILEYKSEFNQKGEVPVDPDYTIPLGVGEIKRQGTDVTVVTYGKMLRR
                     VVQAAEELAEEGISVEIVDPRTLVPLDKDIIINSVKKTGKVVLVNDAHKTSGYIGEIS
                     AIISESEAFDYLDAPIRRCAGEDVPMPYAQNLENAMIPTVESIKDAIRKTYNKE"
     gene            6514..7557
                     /locus_tag="VYN69_001416"
     CDS             6514..7557
                     /locus_tag="VYN69_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008808675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase"
                     /protein_id="HEV1533672.1"
                     /translation="MADDKLRATPAARKLADDLGINLYDVSGSGANGRVHKEDVETYK
                     DTNVVRISPLAKRIALEHNIAWQEIQGTGHRGKIMKKDVLALLPENIENDSIKSPAQI
                     EKVEEVPDNVTPYGEIERIPMTPMRKVIAQRMVESYLTAPTFTLNYEVDMTEMLALRK
                     RVLEPIMEATGKKTTVTDLLSLAVVKTLMKHPYINASLTEDGKTIITHNYVNLAMAVG
                     MDNGLMTPVVYNAEKMSLSELVVAFKDVIGRTLDGKLAPSELQNSTFTISNLGMFGIQ
                     SFGPIINQPNSAILGVSSTIEKPVVVNGEIVIRPIMSLGLTIDHRVVDGMAGAKFMKD
                     LKELIETPISMLI"
     gene            7621..9306
                     /gene="lpdA"
                     /locus_tag="VYN69_001417"
     CDS             7621..9306
                     /gene="lpdA"
                     /locus_tag="VYN69_001417"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014931946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEV1533673.1"
                     /translation="MPKAGVDMTEGQIVQWNKKVGEFVKEGEILLEIMTDKVSMELEA
                     EEDGYLIAILKGDGETVPVTEVIGYLGEERENIPTAGAASPEASPVPVASTSNDDGKS
                     DDAFDIVVIGGGPAGYVAAIKAAQLGGKVALVEKSELGGTCLNRGCIPTKTYLHNAEI
                     IENIGHAANRGIVIENPNFTVDMEKLLETKSKVVNTLVGGVAGLLRSYGVTVHKGIGT
                     ITKDKNVLVNGSELLETKKIILAGGSKVSKINVPGMESPLVMTSDDILEMNEVPESLV
                     IIGGGVVGIELGQAFMTFGSKVTVIEMMDRIVPAMDVEVSKNLRLILERKGMTILTGT
                     KLQEIIEENGQLRIKVEGKDDIIASKALLSIGRMPDLEGIGEVEFELDRGCIKVNEYM
                     ETSVPGIYAPGDINGTKMLAHAAFRMGEVSAENALKGNHAVAKLNLTPAAIYTLPEVA
                     AVGLTEEQAREKYDVAIGKFNFAANGRAIASDAAQGFVKVIADKKYGEILGVHIIGPA
                     AAELINEASSIIEMEITVEEMLKTIHGHPTYSEVMYEAFADVLGMAIHSPKKK"
     gene            9370..10359
                     /locus_tag="VYN69_001418"
     CDS             9370..10359
                     /locus_tag="VYN69_001418"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000873976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEV1533674.1"
                     /translation="MKYIINHSNDTAFNIALEEYAFKHLLDEDQIFLLWINKPSIIVG
                     RHQNTIEEINRDYVRENGIEVVRRISGGGAVYHDLNNLNYTIISKEDENKAFDFKSFS
                     TPVINTLAQLGVKAEFTGRNDLEIDGKKFCGNAQAYINGRIMHHGCLLFDVDLSVLAN
                     ALKVSKDKFESKGVKSVRARVTNIINELPKKITVEKFRDLLLEYMKKEYPEMTEYVFS
                     EEELAKINHIKDTKFGTWDWNYGKSPEFNVRRGTKFTSGKVEVFANVTESKIQDIKIY
                     GDFFGIEDVAAVEDVLRGVKYEREDVLKALKTIDITRYFAGISREEIAEAVVG"
     gene            complement(10432..11502)
                     /gene="xerS"
                     /locus_tag="VYN69_001419"
     CDS             complement(10432..11502)
                     /gene="xerS"
                     /locus_tag="VYN69_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000817875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEV1533675.1"
                     /translation="MKREILLERIDKLKQLMPWYVLEYYQSKLAVPYSFTTLYEYLKE
                     YDRFFSWVLESGISNADKISDIPLSVLENMSKKDMESFILYLRERPLLNANTTKQGVS
                     QTTINRTLSALSSLYKYLTEEVENDQGEPYFYRNVMKKVSTKKKKETLAARAENIKQK
                     LFLGDETEGFLTYIDQEHPQQLSNRALSSFNKNKERDLAIIALLLASGVRLSEAVNLD
                     LRDLNLKMMVIDVTRKGGKRDSVNVAAFAKPYLENYLAIRNQRYKTEKTDTALFLTLY
                     RGVPNRIDASSVEKMVAKYSEDFKVRVTPHKLRHTLATRLYDATKSQVLVSHQLGHAS
                     TQVTDLYTHIVNDEQKNALDSL"
BASE COUNT         3399 a         2172 c         2608 g         3353 t
ORIGIN      
        1 gtattcaaat cgtatatagt cgatttatta aatctttaga taaactgtaa aaagtggttt
       61 ccatagccac tttttgctat aatagaggca gtaaaaatga aggagtcggc tatggaacac
      121 tcgtcttggc atgctttgat taaggcgcaa ttacctgagg gttatttcgg gaaaatcaat
      181 cagtttatgg agcaggtcta ttctcagggg attatttatc cacccaagga aaaggttttt
      241 caggctctct tgacaacact gcttgaagaa gttaaggtgg taattctagg gcaagacccc
      301 tatcacggac caggtcaagc gcagggcttg agtttttccg tacctgactc tatcccagct
      361 ccaccatcct tgcaaaatat cttgaaagaa ttgtcagatg atatcggagt taagaaatct
      421 catgatttga cagcttgggc tgagcaagga gtcttgcttc ttaatgcttg tttgactgtt
      481 cctgctggac aggccaatgg tcatgctggt caaatctggg agccttttac tgatgctgtg
      541 attcaggtgg tcaatcatct agatagacca gtcgtttttg tactctgggg agcttatgca
      601 cgtaagaaga aggccttagt taccaatcct catcacttga ttattgaatc agcccatcca
      661 agtcctttgt cggtttatag aggattttgg ggttccaagc ctttttccaa ggccaataca
      721 ttcttaaaag agacaggaca agagccaatc gattggctta gataaggaga aaatatgcct
      781 cagttagcga cgatttgtta cattgataat gggaaagaac tgctcatgct ccatcgtaat
      841 aagaaaccca atgatgtcca tgaagggaaa tggattggtg tgggtggtaa gctagagaga
      901 ggagagacgc cccaggaatg cgcggtacgt gaaatccttg aagaaacagg gctcaaagcc
      961 aagccagttc taaaaggtgt catcactttt cctgaattta caccagaatt agactggtac
     1021 acctatgttt ttaaggtgac ggagtttgag ggcgacttga ttgactgcaa tgaggggatg
     1081 ctagaatggg ttccctatga tgaggttttg agcaagccga cttgggaagg tgaccacacc
     1141 tttgttgagt ggcttttaga ggataaaccc ttcttttcag ccaagtttgt ttatgatggg
     1201 gataaattgt tggataccca agttgatttc tatgaataaa ggagaaagca gatgctacta
     1261 atcaaaaatg gtcgtgtaat ggatcccaag tctggtttgg atcaagtttg tgatgtctta
     1321 gttcaagatg ggaaaattat caaaattgcg cctgagatca cggaagaagg agcagaaacg
     1381 attgatgcta ctggtcatgt ggttgctcct ggtttggtcg atattcatgt gcatttccgt
     1441 gaacctggtc aaacacataa agaagacatt catactggtg ccctagcagc cgctgcaggt
     1501 ggttttacta ctgtcgtcat gatggctaat accagtccaa ccatttcaga cgtggagact
     1561 ttgcaagcag ttctccagtc agctgccaaa gagaagatta atgtcaagac agttgcgacc
     1621 attactaaaa actttaatgg taaaaacttg actgacttta aggcactttt agaagctggt
     1681 gcggttggtt tctctgatga cggtattccg cttgagagca gtaagattgt caaggaagcc
     1741 atggaggaag ccaaaaaact caataccttt atcagccttc atgaggaaga tccaggtttg
     1801 aacggtgttc ttggctttaa tgaaaatatt gctagagaac atttccatat ctgtggtgct
     1861 actggggtgg ctgagtacgc tatgatggcg cgtgatgtca tgattgccta tgcaactaaa
     1921 gcccatgttc acatccagca tttgtctaag gaagaaagtg ttaaagtagt ggagtttgct
     1981 caggggttag gtgcagaagt cacagcagaa gtagcaccac agcatttctc taagacagaa
     2041 gcacttcttt taacacaagg tagcaatgct aagatgaatc caccgcttcg tttggaatca
     2101 gaccgtcgtg ccgttatcga aggtctcaaa tcaggtgtca tcacagttat tgcgactgac
     2161 cacgcgcctc atcatgtaga tgaaaaaaat gttgaagata ttaccaaagc gccatctggt
     2221 atgactggct tagaaacatc cctgtctctc ggcttgacct atttggtaga agctggtgag
     2281 ttgagcttga tggaattact tgaaaaaatg acatacaacc cagccaagct ttacaacttt
     2341 aaagcaggtt acttggctga gaatggtcca gcagatatca ctatttttga tgccaaggct
     2401 gaccgccttg tggactccca ttttgcttcc aaagcagcta attcaccatt catcggtgaa
     2461 accttaaaag ggcaggttaa atataccatc tgtaagggac aaatcgtcta tcaagcttga
     2521 taggagttag aatatgaatt attttcgaaa acgtagggag agacaagcga aatctaatag
     2581 tggaatttac tgtcccgcag ctaatcgttc caaaattcaa tatgaaacta aagaaaaagc
     2641 tgaccatgcg gttcaatatg ataacggagg tttagttaga agctattatt gtcgtacatg
     2701 tgcgtgttgg catactactt caaaatctaa taaacctccg ctaagtttga aaaactatgg
     2761 tctgaaactc tttggtttag ataaatcaga atagaaagag aagtatgtat aagacaaagt
     2821 gtttacgaga gaagttagta ttatttttaa aaattttctt cccaatcctg atctaccaat
     2881 ttgccaatta ttctgcctct tttgttgata ctgcaatgac aggtcaatac aacactatgg
     2941 acttggctgg tgtatctatg gcaaccagta tctggaatcc tttctttaca tttctaacag
     3001 ggattgtgtc agccttggtg cctatcattg gtcaccatct tggtcgaggc aaaaaggaag
     3061 aagttgcgtc tgatttttac caatttattt atttggcctt gggcctatct gtggtcttgc
     3121 tggggatggt acttttcttg gcaccaacaa tcttgaatca tattgggtta gaagcagcag
     3181 tagcggcagt agcggttcgc tatctttggt ttttatctat cgggattatc cccttgttgc
     3241 tctttagcgt cattcgttcc ttgctggatt cgctgggctt gaccaaactg tccatgtacc
     3301 tcatgctttt gttactccct ctcaatagcg gatttaacta tctcttgatt tacggtgcct
     3361 ttggtgttcc agaactggga ggggctggtg ctggtttagg aacatccttg gcctactggg
     3421 tcttgcttgg gatttctgtt ctggttttat ttaaacagga gaagctcaaa gccttacacc
     3481 ttgagaaacg aattccactt aatatggata acattaagga aggagttcgt ttaggtctgc
     3541 ctattggggg aactgtcttc gcggaagtgg ctatcttttc agtggttggc ttgattatgg
     3601 ctaagttttc gtccttgatt atagctagtc accagtcagc tatgaacttt tcaagtctta
     3661 tgtacgcctt tcctatgagt atctcatcgg ctatggctat tgtcgtttcc tatgaagtgg
     3721 gagccaagcg atttgatgat gcgaaaacct atattggtct aggaagatgg actgctctca
     3781 tttttgcggc cttcacccta accttccttt acatttttag gggaaatgtg gccagtcttt
     3841 atggtaacga cccaaaattt atcgatttga cagcgcgttt tttaacttat agtcttttct
     3901 tccagttagc agataccttt gcggcaccgc ttcagggaat tttgcggggg tataaggata
     3961 cagttattcc tttttacctt ggtttgcttg gttattgggg cgtagcaatc cctgtggcta
     4021 cgctatttga ttccctaaca gattttggag cttattctta ctggatcggc ttgattatta
     4081 gtttgattgt gagtggggcg ctctaccgtt ggcgtttaac tgtgattatg aagagatttg
     4141 aatctttagc aaaatccaaa cgctaaaaag tttgtgaaaa aatatccttg acaagaaaat
     4201 cccttgtttt cacggggttt tcttttttat tttgtgaaat ttcttttaga aaaactttga
     4261 cagtatttgc caaaaacggt aaaatagtaa ggtaagaaga aagttagaaa ggcaagaaga
     4321 tgtcaacttt agataaaaat cttttgctag aaatgttccg taagatggaa gaaatccgtc
     4381 gcatggactt aaaaattgca caattagtaa agaaagggaa agtgccagga atgacgcact
     4441 tttctgttgg tgaagaggca gctaacgtgg gggctatgtt agctctcaat ccagatgatc
     4501 tgattacctc aaaccaccgt ggacacgggc aagctattgc taaagggatt gacctcaacg
     4561 gaatgatggc tgaaatcctt ggtaaataca ctggaacctg taaagggaaa ggtggatcta
     4621 tgcatatcgc tgaccttgat gctgggaacc ttggtgccaa tggtatcgta ggtggtggta
     4681 tggggatcgc tgtcggtgca gccctcagtc agcaaatgca aaataccggt aaaatcgttg
     4741 tttgcttctt tggagatggt gcgaccaatg aaggtgtttt ccacgaagca gtgaacatgg
     4801 cttctatctg gaacctgcca gtcattttct attgcattaa caacggttac ggtatctctg
     4861 cggatatcaa gaaaatgacc aatatagaac atatccatca acgtagcgcc gcttatggaa
     4921 ttcctggaat gttcatcgaa gacggtaaca atgtcatcga tgtctatgaa ggatttcaga
     4981 aagctgtaga ccatgttcgc agtggcaatg gtccagtctt gattgaaagt gtaacttatc
     5041 gctggctcgg tcactcatca tctgaccctg gtaaatatcg tacgcgtgaa gaagtggaat
     5101 tgtggaaaca aaaagatcca atcgaaaacc tccgcaatta ccttattgaa aataacattg
     5161 caagtgccga agaattggaa gaaatccaag cgcaagtaaa ggaagcagta gaagcttctg
     5221 ttaaatttgc agaggaaagc ccattcccac cgcttgaatc agcctttgaa gatatttacg
     5281 cagactaagg agaaagagat aaaatggaaa caaaaacaat gtccttccgt gacaccatta
     5341 tccttgctat gtctgaggaa atgcgtcgcg atgaaaatgt gttcttgatg ggagaagacg
     5401 tcggtgtctt cggaggagac ttcggtactt ctgttggaat gcttgaagaa tttggtccag
     5461 aacgcgtccg tgactgtccg atttctgaag ctgccatctc tggagcagca gcaggagcag
     5521 ccatgacagg acttcgtcca atcgtcgata tgaccttcat ggacttctct gttattgcca
     5581 tggacaatat cgtcaaccaa gctgctaaaa cacgttacat gtttggtggt aaaggtcagg
     5641 ttccaatgac tgttcgttgt gcagctggta acggagttgg ctctgccgcc cagcactcac
     5701 aatctctaga gtcttggttt actcacattc caggacttaa ggttgtggca cctggaacac
     5761 ctgcggacat gaaaggcctg cttaagtctt ctatccgtga taaaaaccca gttattatac
     5821 ttgagtacaa gtcagaattt aaccaaaaag gggaagtgcc agttgatcca gactacacaa
     5881 ttccacttgg ggttggtgaa atcaaacgcc aaggaacgga tgtaacagtt gttacttatg
     5941 gaaaaatgct tcgccgtgtg gttcaagctg ctgaagaatt agcagaagaa ggaatttcag
     6001 ttgaaattgt tgacccacgt acccttgttc cgcttgataa ggatatcatc ataaactcag
     6061 tgaagaagac tggtaaggtt gttctggtca acgatgccca caaaacaagt ggctatatcg
     6121 gtgaaatttc agctattatt tcagaatcag aagcatttga ctatctagat gcaccaatcc
     6181 gccgttgtgc aggagaagat gtgccgatgc cttatgcaca aaacctagaa aatgcaatga
     6241 ttccaacagt tgaaagtatc aaagatgcaa tccgaaaaac ttataacaaa gaatagagtt
     6301 acataagata agttatgtaa ataaaataaa gctgacgaga aactttgtat cttttaggtg
     6361 cagagctctt cgtcattaat atcttagatt agagcacggg ctaaaagctc ggaaaaaaga
     6421 taaatctcct tggcttcatc gaagccatcg tcgatttcct atttttcagt cgcttttgac
     6481 gccctttgta tcataaatag aattggagag gtcatggctg atgacaagct aagagcgact
     6541 cctgcagcta gaaagttagc ggatgattta gggataaacc tctacgacgt ttctggctca
     6601 ggtgcaaacg gtcgtgtcca caaagaagac gtggaaactt ataaagacac aaacgtggtt
     6661 cgcatttcgc cacttgcaaa acgaattgcc ctcgaacata acattgcttg gcaggaaatc
     6721 caaggaaccg gtcatcgtgg taaaatcatg aagaaggatg ttttggccct gcttcctgaa
     6781 aatatcgaaa acgatagcat caaatctcct gctcagattg aaaaagtgga agaagttcct
     6841 gataacgtaa caccatatgg tgaaatcgag cgtattccaa tgacaccaat gcgtaaggtt
     6901 attgcccaac gcatggttga atcttacttg actgcgccaa ccttcaccct caactatgaa
     6961 gttgatatga ctgaaatgtt ggctcttcgt aagagggttc ttgagccaat catggaagca
     7021 actgggaaga agactactgt aacagacctt ctttcacttg cagttgttaa gactcttatg
     7081 aaacatccat acatcaacgc ttcattgaca gaagatggca agactattat cactcacaac
     7141 tatgtcaatc ttgctatggc agttgggatg gataatggat tgatgacacc tgttgtttac
     7201 aatgctgaga agatgagtct ttcagaactg gttgtagcct ttaaggatgt tattggccgt
     7261 accttggatg gtaaattggc tccaagtgag ttacaaaatt caacattcac aatcagtaat
     7321 ttgggaatgt ttggtattca gtcctttggt ccgattatta accaacccaa ctcagctatc
     7381 cttggtgtca gttcgacaat cgagaagcca gttgtcgtca atggtgaaat tgtgattcgc
     7441 ccaatcatga gtttaggatt aaccattgac caccgtgtcg tagatggtat ggctggtgct
     7501 aagtttatga aggacttgaa agagttgatt gaaaccccaa tctcaatgtt gatttaagag
     7561 caagagtatt catttttaag atatagataa aggaaggaag acatggcctt agaaataatt
     7621 atgccaaaag ccggcgtgga tatgacagaa ggacaaatcg tccaatggaa taaaaaagtc
     7681 ggagaatttg taaaagaagg agaaatcctt ttggaaatca tgactgataa agtcagcatg
     7741 gaattggaag ccgaagaaga tgggtacttg attgccattc tcaaaggaga tggtgaaact
     7801 gtccctgtaa cggaagttat cggttacctt ggtgaagaaa gggaaaacat cccaacagct
     7861 ggagcagcat cgccagaagc tagccctgta cctgtagcaa gtacctcaaa cgatgatggt
     7921 aagagcgatg atgcttttga tatcgttgtg attggtggag gtcctgctgg ttatgttgca
     7981 gccattaaag ctgcccaact cggcggtaag gttgcccttg ttgagaaatc tgaacttggt
     8041 ggaacctgct taaaccgtgg atgtattcca accaagacct accttcataa cgctgaaatt
     8101 attgaaaata tcggtcatgc tgcaaatcgt ggtatcgtga ttgaaaatcc taacttcact
     8161 gttgatatgg aaaaactttt agaaactaaa tctaaagttg ttaatactct tgttggtggt
     8221 gttgcaggac ttcttcgtag ctacggagtt actgttcata aaggaattgg tacaatcact
     8281 aaagacaaga acgtcttggt aaatggttct gaattgcttg aaaccaagaa aatcattctt
     8341 gctggtggtt caaaagtcag caagatcaac gtccctggta tggaatctcc acttgtcatg
     8401 actagtgatg acattcttga aatgaacgaa gtgccagaaa gccttgttat catcggtggt
     8461 ggagttgtcg gtatcgaact cggtcaggcc ttcatgacat ttggttcaaa agtgactgtt
     8521 atcgaaatga tggaccgtat agttccagct atggatgtgg aagtttctaa gaaccttcgc
     8581 ttgatccttg agcgtaaagg aatgaccatc ttgactggta ctaaactgca agaaatcatt
     8641 gaggaaaatg gtcaacttcg tatcaaggtt gaaggaaaag acgatatcat cgcaagcaaa
     8701 gctcttcttt caattggtcg tatgccagac cttgaaggta ttggagaggt tgagtttgaa
     8761 ttggatcgtg gttgtatcaa ggtcaacgaa tacatggaaa cttcagttcc aggcatttat
     8821 gcaccaggtg acatcaacgg tactaagatg ttggctcacg cagctttccg catgggtgaa
     8881 gtttccgctg aaaatgccct taaaggaaat catgcagttg ccaaattgaa tttgactcct
     8941 gcagccatct acactctccc tgaagtagca gcagtaggtt tgacagaaga acaagcccgt
     9001 gagaaatacg atgttgccat cggtaagttt aactttgctg ctaacggtcg tgctattgca
     9061 tctgacgcag ctcaaggttt cgtaaaagtt atcgctgata agaaatacgg agaaatcctt
     9121 ggtgtacaca ttattggtcc tgcagccgca gaattaataa acgaagcatc aagcatcatc
     9181 gaaatggaaa tcactgttga ggaaatgctg aagaccatcc acgggcaccc aacttactct
     9241 gaagtgatgt acgaagcgtt tgcagatgtt ctaggaatgg ccatccattc acctaagaaa
     9301 aaataaaacg aagatagatt agactggaag gttatttcca gtttctatca tataaagtaa
     9361 agggatatca tgaaatacat tatcaatcat tcaaatgaca ctgcttttaa tattgccttg
     9421 gaagaatacg cctttaaaca ccttttggat gaggatcaaa tcttcctact ttggattaac
     9481 aagccatcta tcattgttgg tcgtcaccag aacactatcg aagaaatcaa ccgtgattat
     9541 gttcgcgaaa atggtattga ggtagttcgc cgtattagtg gtggtggagc tgtttaccac
     9601 gatttaaata acctcaacta cacgatcatc tccaaagaag atgaaaataa ggcatttgac
     9661 ttcaagagct tctcaactcc ggttatcaat accttggctc aacttggggt taaagctgag
     9721 ttcacaggtc gtaatgacct tgagattgat ggtaagaagt tctgtggcaa tgcccaagcc
     9781 tatatcaacg gccgtatcat gcaccacggt tgcttgctct ttgacgttga tttgtcagtc
     9841 ctcgcaaatg cccttaaggt ttcaaaagat aaatttgaat caaaaggtgt gaaatccgtc
     9901 cgtgcccgtg taaccaatat tatcaatgaa ttaccaaaaa aaatcacagt cgaaaaattc
     9961 cgtgatttac tcttggaata catgaaaaaa gagtacccag agatgactga atacgtcttt
    10021 tcagaagaag aattggccaa aatcaatcac atcaaggata ctaagtttgg aacttgggac
    10081 tggaactatg gtaaatcacc tgaatttaac gtccgtcgtg gaaccaaatt cactagtggt
    10141 aaggttgaag tctttgctaa cgttactgaa tcaaaaatcc aagacatcaa gatttatggt
    10201 gacttctttg gtattgaaga cgttgctgca gtagaagatg tccttcgtgg ggtgaaatac
    10261 gaacgcgaag atgttcttaa ggcgctaaaa accattgata tcacacgcta cttcgctggt
    10321 attagccgtg aagaaatcgc tgaagcggta gttggataaa tcaaaaagaa gttggccaac
    10381 taaccaactt ctttttttga tatttattta cataatttaa aatacgtaaa attataaact
    10441 atctagagca ttcttttgtt catcattaac aatatgggta tagaggtcag tgacttgtgt
    10501 gctggcatgt cctagttggt gactgactaa aacttgtgat ttagtcgcat catagagcct
    10561 agttgctagc gtatggcgca gtttatgggg tgttacacgc actttaaaat cctctgagta
    10621 tttagcaacc attttctcaa cgctagaagc atcgatacga ttaggaacac ctctgtagag
    10681 agttaaaaaa agggctgtat ctgttttttc cgttttatag cgttgattcc gaatggccag
    10741 ataattctct aaataaggtt tagcaaaagc agcgacattg actgagtcac gtttgccacc
    10801 ttttcgagta acatcaataa ccatcatttt tagattgaga tctcttagat ctagattaac
    10861 agcttcagat aagcgaacac cagatgccaa gagaagggca ataatggcta aatctcgttc
    10921 tttatttttg ttgaatgatg agagagctcg atttgaaagc tgttgtgggt gctcttgatc
    10981 gatataagtt agaaaacctt ctgtttcatc acctagaaag agtttttgct tgatattttc
    11041 agctctggca gcaagggttt ctttcttttt cttggttgaa acttttttca ttacattacg
    11101 atagaaataa ggttccccct gatcgttttc aacctcctcg gttagatact tgtaaagact
    11161 agaaagtgct gataaggttc gattgatagt tgtctgtgaa acaccctgtt ttgttgtatt
    11221 agcattcagc aagggacgtt cacgtagata aaggataaag gattccatgt ctttcttaga
    11281 catattttcc aaaactgata aaggaatatc ggatatttta tcagcgtttg aaataccaga
    11341 ctccaaaacc cagctgaaaa atcggtcata ttccttaagg tattcgtaca gggttgtaaa
    11401 actgtagggt acagccagct tagattggta gtattccaga acataccagg gcatgagttg
    11461 ttttagtttg tcgattcgtt ccagtaaaat ctcacgtttc atgtattttc tccataagta
    11521 agtatactag ta
//
LOCUS       DAVLVP010000056        11324 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.056, whole genome shotgun sequence.
ACCESSION   DAVLVP010000056 DAVLVP010000000
VERSION     DAVLVP010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 11324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.056"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            1..1061
                     /locus_tag="VYN69_001420"
                     /pseudo
     CDS             1..1061
                     /locus_tag="VYN69_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1104..1745)
                     /gene="plsY"
                     /locus_tag="VYN69_001421"
     CDS             complement(1104..1745)
                     /gene="plsY"
                     /locus_tag="VYN69_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEV1533676.1"
                     /translation="MITIVLLILAYLLGSIPSGLWIGQVFFQINLREHGSGNTGTTNT
                     FRILGKKAGMATFVIDFFKGTLATLLPIIFHLQGVSPLIFGLLAVIGHTFPIFAGFKG
                     GKAVATSAGVIFGFAPIFCLYLAIIFFGALYLGSMISLSSVTASIAAVIGVLLFPLFG
                     FILSNYDFLFIAIILALASLIIIRHKDNIARIKNKTENLVPWGLNLTHQDPKK"
     gene            1881..3824
                     /gene="parE"
                     /locus_tag="VYN69_001422"
     CDS             1881..3824
                     /gene="parE"
                     /locus_tag="VYN69_001422"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEV1533677.1"
                     /translation="MSKKEININNYNDDAIQVLEGLDAVRKRPGMYIGSTDGAGLHHL
                     VWEIVDNAVDEALSGFGDRIDVTINKDGSLTVQDHGRGMPTGMHAMGIPTVEVIFTIL
                     HAGGKFGQGGYKTSGGLHGVGSSVVNALSSWLEVEITRDGAVYKQRFENGGKPVTTLK
                     KVGTAPKSKTGTKVTFMPDATIFSTTDFKYNTISERLNESAFLLKNVTLSLTDKRTDE
                     AIEFHYENGVQDFVSYLNEDKEILTPVLYFEGEDNGFQVEVALQYNDGFSDNILSFVN
                     NVRTKDGGTHETGLKSAITKVMNDYARKTGLLKEKDKNLEGSDYREGLAAVLSILVPE
                     EHLQFEGQTKDKLGSPLARPVVDGIVADKLTFFLMENGELASNLIRKAIKARDAREAA
                     RKARDESRNGKKNKKDKGLLSGKLTPAQSKNPAKNELYLVEGDSAGGSAKQGRDRKFQ
                     AILPLRGKVINTAKAKMADILKNEEINTMIYTIGAGVGADFSIEDANYDKIIIMTDAD
                     TDGAHIQTLLLTFFYRYMRPLVEAGHVYIALPPLYKMSKGKGKKEEVAYAWTDGELEE
                     LRKQFGKGATLQRYKGLGEMNADQLWETTMNPETRTLIRVTIEDLARAERRVNVLMGD
                     KVEPRRKWIEDNVKFTLEEATVF"
     gene            4244..6715
                     /gene="parC"
                     /locus_tag="VYN69_001423"
     CDS             4244..6715
                     /gene="parC"
                     /locus_tag="VYN69_001423"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEV1533678.1"
                     /translation="MSNIQNMSLEDIMGERFGRYSKYIIQDRALPDIRDGLKPVQRRI
                     LYSMNKDSNTFDKSYRKSAKSVGNIMGNFHPHGDSSIYDAMVRMSQNWKNREILVEMH
                     GNNGSMDGDPPAAMRYTEARLSEIAGYLLQDIEKKTVPFAWNFDDTEKEPTVLPAAFP
                     NLLVNGSTGISAGYATDIPPHNLAEVIDAAVYMIDHPTAKIDKLMEFLPGPDFPTGAI
                     IQGRDEIKKAYETGKGRVVVRSKTEIEKLKGGKEQIVITEIPYEINKANLVKKIDDVR
                     VNNKVAGIAEVRDESDRDGLRIAIELKKDANTELVLNYLFKYTDLQINYNFNMVAIDN
                     FTPRQVGIVPILSSYIAHRREVILARSRFDKEKAEKRLHIVEGLIRVISILDEVIALI
                     RASENKADAKENLKVSYDFTEEQAEAIVTLQLYRLTNTDVVVLQEEEAELREKIAMLA
                     AIIGDERTMYNLMKKELREVKKKFATPRLSSLEDTAKAIEIDTASLIAEEDTYVSVTK
                     AGYIKRTSPRSFAASTLEEIGKRDDDRLIFVQSAKTTQHLLMFTSLGNVIYRPIHELA
                     DIRWKDIGEHLSQTITNFETNEEILYVEVLDQFDDATTYFAVTRLGQIKRVERKEFTP
                     WRTYRSKSVKYAKLKDDTDQIVAVAPIKLDDVVLVSQNGYALRFNIEEVPVVGAKAAG
                     VKAMNLKEDDVLQSGFICNTSSFYLLTQRGSLKRVSIEEILATSRAKRGLQVLRELKN
                     KPHRVFLAGVVAEQGFVGDFFSTEVDVNDQTLLVQSNKGTIYESRLQDLNLSERTSNG
                     SFISDTISDEEVFDAYLQEVVTEYK"
     gene            6847..7869
                     /locus_tag="VYN69_001424"
     CDS             6847..7869
                     /locus_tag="VYN69_001424"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEV1533679.1"
                     /translation="MTVTIDWENLGFSYMKLPYRYLAHFKNGQWDQGELTEDATLHIS
                     ESSPSLHYGQQAFEGLKAYRTKDGSVQLFRPDENAKRLQRTCDRLLMPQVPTDMFVEA
                     CKAVVRANEEYVPPYGTGGTLYLRPLLIGVGDIIGVKPAEEYIFTIFAMPVGNYFKGG
                     LVPTNFLIQDEYDRAAPNGTGAAKVGGNYAASLLPGKMAKSRHFSDVIYLDPSTHTKI
                     EEVGSANFFGITADNEFVTPLSPSILPSITKYSLLYLAEHRLGLTPIEGDVPIDNLDR
                     FVEAGACGTAAVISPIGGIQHGDDFHVFYSETEVGPVTRKLYNELTGIQFGDIEAPEG
                     WIVKVD"
     gene            7925..8629
                     /locus_tag="VYN69_001425"
     CDS             7925..8629
                     /locus_tag="VYN69_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1533680.1"
                     /translation="MVLSTSAILVACGKTDKEADAPTTFSYVYAVDPASFGYSIATRT
                     SRTDVIGNVIDGLMENDKYGNVAPSQKDYDLNSTGWAPSYQDPASYLNIMDPKSGSAM
                     KHLGITKGKDKDVVAKPGLDKYKKLLEDAVSEITDLEKRYEKYAKAQAWSTDSSLLMP
                     TASSGGFPVVSNVVPFSKPYSQVGIKGEPYIFKGMKLQKDIVTTKEYNEVFKKWQKEK
                     LESNSKYQKELEKYIK"
     gene            8694..9383
                     /locus_tag="VYN69_001426"
     CDS             8694..9383
                     /locus_tag="VYN69_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEV1533681.1"
                     /translation="MEWIRLIGIAIIVVGFILKFDTIATVVLAGLVTALVSGVSLVEF
                     LEILGKEFSNQRVLTIFMVTLPLVGLSETFGLKQRSIDLIRKIKGLTVGNFYTVYFFI
                     RELAGFFSIRLGGHPQFVRPLVQPMGEAAAESQLGRKLTEVEDETIKARAAANENFGN
                     FFAQNTFVGAGGVLLIGGTLDQLGYESNYAGIASTSIIVAVITLIVVGIYNYLFDKKL
                     ISKKTRGGEQK"
     gene            9380..10303
                     /locus_tag="VYN69_001427"
     CDS             9380..10303
                     /locus_tag="VYN69_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEV1533682.1"
                     /translation="MTELAKQLLELTYIVIGCQFLHTAYCSYKDKTNPVRLGTSAFWT
                     LLSITFIGGSYMPNMSIGIIVILLSLLTLFKQVRIGTLPSLDEMKANIESNRLKNKIF
                     IPVMLMAILALVLAQMIPEFSKISISLAALFATISVLVITNSHPKSLLSENNRMTQQV
                     STSGIVPQLLGALGAIFTVAGVGDVISHLISGIVPSDSRFIGVLAYVLGMVLFTMIMG
                     NAFAAFTVITAGVGVPFVFALGANPIVAGALAMTAGYCGTLLTPMAANFNALPAALMD
                     MKDQNGVIKAQAGVALVMIVIHIFLMYFLAF"
     gene            10318..10962
                     /gene="pcp"
                     /locus_tag="VYN69_001428"
     CDS             10318..10962
                     /gene="pcp"
                     /locus_tag="VYN69_001428"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEV1533683.1"
                     /translation="MKILVTGFNPFGGEKINPALEAVKLLPSEINGAEVRWVEIPTVF
                     YKSSEVLEAEILRYQPDAVLCIGQAGGRTGLTPERVAINQDDARIPDNEGNQPIDTPI
                     RIDGASAYFSSLPIKAMVQAIKKQGLPAVVSNSAGTFVCNHLMYQALYLVDKKFPNMR
                     AGFMHIPYMMEQVVNKPNTAGMSLCDIVRGIEVAIEAIVDYKDKDLQLVGGETH"
     gene            11130..>11324
                     /locus_tag="VYN69_001429"
     CDS             11130..>11324
                     /locus_tag="VYN69_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000037109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEV1533684.1"
                     /translation="MSKKDKKIEIQVADAKVNVGKDSFEGYTLTIGKKVIGEIAELDG
                     QFAIIKNGNVDSFYKKLEKAV"
BASE COUNT         3577 a         2006 c         2540 g         3201 t
ORIGIN      
        1 ttaactggct agcacaatac agaaaaaacg ggtatactat tgttgagaaa ccaagaggga
       61 gagtacctaa aatgggacgt aagccaagaa cgagacctga agagaggaca gaattagaac
      121 gtcttcaagc agaaaatgag tacctgagag cggagaatgc catcctaaaa aagttaagag
      181 aactccgatt gaaggaggaa aaagagaaag aagaaagaca gaaattgttc aagaattaat
      241 gactgagttt tcgttagata ttcttctaaa agccattaag ctagctcgtt ggacctacta
      301 ctatcacttg aaacagttag acaaaccaga taaggaccaa gagcttaaag ctgaaattca
      361 atccattttt atcgaacaca agggaaatta tggttatcgt cgaatttatt tagaactaag
      421 aaatcgtggt tatctggtaa atcataaaag agttcaaggc ttgatgaaag tactcaattt
      481 acaagctaaa atgcgacaga aacgaaaata aaggagacgt tggtaagaag gcagagaatc
      541 tcattcaagg ccaatttgaa ggctctaaaa caatggaaaa gtgctacaca gatgtgacag
      601 aatttgccat tccagcaagt actcaaaagc tttacttatc accagtttta gatggcttta
      661 atagcgaaat tatcgcctat aatctttcaa cttcgcccaa cttagaacaa gtgaagagta
      721 tgttagaaca ggcctttaca gagaaatact atgagaatac gattctccat agtgaccaag
      781 gctggcaata ccgacacgat tcttatcatc ggttcctaga gagtaaggga gttcaagcat
      841 ccatgtcacg taagggtaac agcccagaca atggtatgat ggagtccttc tttgggattc
      901 tgaaatcgga aatgttttac ggttatgaga agtcgtttca gtcgcttaag caattggaac
      961 aagccattat agactatatt gattactaca acaataaacg aattaagata aaactaaaag
     1021 gacttagccc tgtgcaatac agaactaaat ccttcggata aattaattgt ctaactgttt
     1081 ggggtcagta cagaactggc gttttatttt ttaggatctt gatgggttag gttcaatccc
     1141 caagggacca aattttcagt tttatttttg atacgagcta tattgtcctt atgacgaatg
     1201 ataatcaaac tagcaagtgc taagataata gcgatgaaga gaaagtcata gttactcagg
     1261 ataaaaccaa aaagtggaaa gagcagaacc ccgataacag ccgcaatcga tgctgtgaca
     1321 ctagacagtg aaatcatact gccaagatag agagctccaa agaagataat cgcaaggtag
     1381 agacagaaga taggcgcaaa tccgaaaatc actccagcac tggttgcgac agccttacca
     1441 cctttaaatc ctgcaaagat agggaaggta tggccgataa cagccaaaag tccaaagatg
     1501 agaggagaaa cgccttgtag atgaaaaata atcggaagca gcgttgctag ggttcctttg
     1561 aaaaagtcaa tcacaaaggt tgccatacca gctttcttac ctaaaatgcg gaaggtattg
     1621 gtcgttccag tgttaccaga accatgctcg cgtagattga tttgaaagaa tacttgtcca
     1681 atccagagac cagatggaat cgaacccagc agataggcta ggattaataa aactattgta
     1741 atcatactcc tattatatca tgaattggga aagaaaagca gagaatctct gctaaaattg
     1801 tcacatcgga gaaagaaaaa ttttgcaaaa tccttggaaa acctgtagaa tagtaaagat
     1861 gaacgaatag gaggttcctt gtgtcaaaaa aggaaatcaa tattaacaat tataatgatg
     1921 atgctattca ggtgctagaa gggttggatg cggtccgaaa acgtccaggg atgtatattg
     1981 gatcgaccga tggcgctggt cttcatcacc tagtttggga aatcgttgat aatgcagtcg
     2041 atgaagcctt gtctgggttt ggtgatcgta ttgatgtaac tatcaataaa gacggtagtc
     2101 taacggttca agaccatgga cgtgggatgc cgacaggtat gcacgctatg ggaattccaa
     2161 ctgttgaggt tatctttacc attcttcatg ccggagggaa attcggtcaa ggtggctata
     2221 agacatcagg tggacttcac ggagtgggtt cttccgttgt taacgccctt tctagctggt
     2281 tagaagttga aattacccgt gatggcgcag tttacaagca acgttttgaa aatggtggaa
     2341 aacctgtcac gactttgaag aaagtcggta cagcacccaa gtctaaaaca ggcaccaaag
     2401 ttacttttat gcctgacgcg actatctttt ctacgacaga tttcaagtac aataccattt
     2461 cagagcgcct taatgaatca gcctttctct tgaaaaatgt gaccttgtct ttaacggaca
     2521 agcgaacaga tgaagcgatt gagttccact atgagaatgg agtacaagat tttgtttctt
     2581 atctcaacga agataaggaa atcttgacgc cagttcttta ctttgaaggg gaagacaatg
     2641 gttttcaagt ggaagtagcc ctccagtaca atgacggatt ctcagataac attctatcct
     2701 ttgtcaataa cgttcgcacc aaggacggtg gaacgcacga gacaggactc aagtctgcca
     2761 ttaccaaggt catgaatgac tatgcacgta aaacaggtct tctcaaggaa aaagataaaa
     2821 accttgaagg ttcagactat cgtgagggac tagcggccgt tctttctatc ttagttcctg
     2881 aagaacactt gcagtttgaa ggacagacca aggataaact aggaagcccc ctagctcgcc
     2941 cagttgtgga tggaatagtg gctgataagt tgaccttttt ccttatggaa aatggggaat
     3001 tagcttctaa cctcatccgc aaggctatca aggcccgtga tgctcgtgaa gcagcacgta
     3061 aggcgcgtga tgagagccga aatgggaaga aaaacaagaa agataagggc ttgttgtctg
     3121 ggaaattgac cccagcccaa tctaagaatc ctgctaagaa tgaactctat ctagttgagg
     3181 gggactctgc cggtggttct gccaaacaag gtcgtgaccg caagttccag gctattctac
     3241 ctcttcgtgg taaggttatc aatacagcca aggccaagat ggcggatatc ctcaaaaatg
     3301 aagagatcaa taccatgatt tataccattg gtgcgggtgt tggagcagac ttctctattg
     3361 aagatgccaa ctatgataag atcattatca tgaccgatgc ggataccgac ggtgcccata
     3421 tccagacctt gctcttgaca tttttctacc gttacatgcg tccgctagtc gaggcaggtc
     3481 atgtctatat tgccctccca cctctttaca agatgtccaa aggtaaaggc aagaaagaag
     3541 aagtggccta cgcttggacg gacggagaac tagaagaact ccgtaaacag ttcggtaaag
     3601 gcgctaccct ccaacgatac aaaggacttg gtgagatgaa tgcggaccag ctctgggaaa
     3661 caaccatgaa cccagaaaca cgtaccctca tccgtgtcac aattgaagat ttagcgcgcg
     3721 ccgaacgccg cgtcaatgtt ctcatgggag ataaggtaga accacgccgt aaatggattg
     3781 aagataatgt caagtttacg ctagaagaag cgacagtgtt ttaatgaaag aaaataatat
     3841 gttagtaaac tggaaattag agtcggatgt gaatgactat gtgaaaaagc agtttgaaaa
     3901 tcttggtttg aaaaaattgc aggattatta atgaaggatc tgctatgagt gcttatctta
     3961 aagaggcgct taagggagcg gttaagacac aaactaagac taattttgga aaaccagatt
     4021 ttcatattga aaaatacaaa caggatagtt tggttactta tcgtaactga acacgggacg
     4081 aattcccgta aaatatcttt tactgcataa aaacctactc tacattcttt gaaaggagtt
     4141 gaacacgccc tagatactgt gtgaaaaaga taaattctct tgtgagtttg cttacttcaa
     4201 gaattttcta ttttcacttg gtattttatg ggctttgtat cttatgtcta acattcaaaa
     4261 catgtccctg gaggacatca tgggagagcg ctttggtcgc tactccaagt acattattca
     4321 agaccgggct ttgccagata ttcgtgatgg gttgaagccg gttcaacgcc gtattcttta
     4381 ttctatgaat aaggatagca atacttttga caagagctac cgtaagtcgg ccaagtcagt
     4441 cgggaacatc atggggaatt tccacccaca cggggattct tctatctatg atgccatggt
     4501 tcgtatgtca cagaactgga aaaatcgtga gattctagtt gaaatgcacg gtaataacgg
     4561 ttctatggac ggagatcctc ctgcggctat gcgttatact gaggcacgtt tgtctgaaat
     4621 tgcaggctac cttcttcagg atatcgagaa aaagacagtt ccttttgcat ggaactttga
     4681 cgatacggag aaagaaccaa cggtcttgcc agcagccttt ccaaacctct tggtcaatgg
     4741 ttcgactggg atttcggctg gttatgccac agacattcct ccccataatt tagctgaggt
     4801 catagatgct gcagtttaca tgattgacca cccaactgca aagattgata aactcatgga
     4861 attcttacct ggaccagact tccctacagg ggctattatt cagggtcgtg atgaaatcaa
     4921 gaaagcttat gagactggga aagggcgcgt ggttgttcgt tccaagactg aaattgaaaa
     4981 gctaaaaggt ggtaaggaac aaatcgttat tactgagatt ccttatgaaa tcaataaggc
     5041 caatctagtc aagaaaatcg atgatgttcg tgttaataac aaggtagctg ggattgctga
     5101 ggttcgtgat gagtctgacc gtgatggtct tcgtatcgct atcgaactta agaaagacgc
     5161 taatactgag cttgttctca actacttatt taagtacacc gacctacaaa tcaactacaa
     5221 ctttaatatg gtggcgattg acaatttcac acctcgtcag gttgggattg ttccaatcct
     5281 gtctagctac atcgctcacc gtcgagaagt gattttggcg cgttcacgct ttgacaaaga
     5341 aaaggctgag aaacgtctcc atatcgtcga aggtttgatt cgtgtgattt cgattttgga
     5401 tgaagtcatt gctcttatcc gtgcttctga gaataaggcg gacgccaagg aaaacctcaa
     5461 agttagctat gattttacgg aagaacaggc tgaggctatc gtaactttgc aactgtaccg
     5521 tttgaccaat accgatgtgg ttgtcttgca ggaagaagaa gcagagcttc gtgagaagat
     5581 tgctatgctg gcggctatta tcggtgatga aaggactatg tacaatctca tgaagaaaga
     5641 acttcgtgag gtcaagaaga aatttgcaac tcctcgtttg agttctttag aagacactgc
     5701 gaaagcaatt gagattgata cagctagtct tatcgctgag gaagatacct acgtcagcgt
     5761 gaccaaggca ggttacatca agcgtaccag tccacgttcc tttgcggctt ccaccttgga
     5821 agaaattggc aagcgtgatg atgaccgttt gatttttgtt caatctgcca agacaaccca
     5881 gcacctcttg atgttcacaa gtcttggaaa tgtcatctac agaccaatcc atgagttggc
     5941 agatattcgt tggaaggaca tcggagagca tctgagccaa accatcacaa actttgaaac
     6001 gaatgaagaa atcctttatg tggaagtact ggatcagttt gacgatgcga caacctactt
     6061 tgcagtgact cgccttggtc aaatcaaacg ggtagagcga aaagaattca ctccatggcg
     6121 gacctataga tctaagtctg tcaagtatgc taagctcaaa gacgatacag atcagattgt
     6181 agcagtggct ccgattaaac tagatgatgt tgtcttggtt agtcaaaatg gttatgccct
     6241 gcgtttcaat atcgaagagg ttccggttgt cggtgctaag gcagcaggtg tcaaggctat
     6301 gaatttgaaa gaagatgatg tcctccaatc tggctttatc tgtaatactt cgtccttcta
     6361 cctcttgacc cagcgtggaa gcttgaaacg tgtttctatt gaggaaattc tagcaaccag
     6421 ccgtgccaaa cgaggattac aagtcttgcg tgagttgaaa aacaaaccgc atcgtgtctt
     6481 cttggcagga gtagttgcag agcaaggatt tgttggcgat ttcttcagta cggaagtgga
     6541 tgtgaacgac caaactctgc ttgtccaatc caataaagga acaatctatg aaagccgatt
     6601 gcaagacttg aacttgtcag aacgcactag caatggaagc ttcatttctg acacgatttc
     6661 agatgaagaa gtttttgacg cttatcttca ggaagtagtt actgaatata aataagtgaa
     6721 atgaagaata agttctaaga gctggttttt attggagaaa ttttctgaaa attacaaaat
     6781 atacttgcta ttttagaaaa atagtgtaga atataagaaa taggttttta gaataaggag
     6841 tggaatatga cagtaacgat tgattgggaa aacctcggtt tttcctatat gaaattacct
     6901 tatcgctatc ttgctcattt caaaaatgga caatgggatc aaggagagct tacagaggat
     6961 gcaactttgc atatttcaga gtcttctcca agtcttcact atggacaaca agcatttgaa
     7021 ggtttgaaag cttatcgtac taaggatggc agtgttcaac tgttccgtcc tgatgaaaat
     7081 gctaaacgtc tgcaacgtac atgtgaccgt ctcttgatgc cacaagttcc gacagacatg
     7141 tttgtagaag cttgtaaagc agttgtccgt gcgaatgaag aatacgtacc accatacgga
     7201 acaggtggaa ccttatatct tcgccctctt ttgattggtg tcggagatat tatcggggta
     7261 aaaccggcag aagagtacat tttcaccatc tttgctatgc cagttggaaa ttactttaaa
     7321 ggtggtttgg tcccaaccaa cttcttgatt caggatgagt acgaccgtgc agcaccaaat
     7381 ggtacaggtg cggctaaggt tggtggaaac tatgctgcaa gtctcttacc aggaaaaatg
     7441 gccaagtcac gtcatttctc agatgttatc tatctggacc catcaactca tacaaagatt
     7501 gaagaagtcg gatcagctaa cttctttgga attacagctg ataatgaatt tgtaacacca
     7561 ttgagtccat ctatcttgcc atctattacc aagtattcct tgctttattt ggcagaacat
     7621 cgcttgggat taactcctat tgagggtgat gttccaattg ataatcttga ccgttttgta
     7681 gaggcaggtg cctgtggtac agcagcggtt atttctccaa ttggaggtat tcaacatggt
     7741 gatgatttcc atgtattcta tagtgaaaca gaagtaggtc ctgtgacgcg taaattatat
     7801 aatgaattga cgggtattca gtttggcgat attgaagcgc cagaaggttg gattgtaaaa
     7861 gtagattaaa ataaaccaaa ggagattttt tatgaaatag aaaaagtggc tcttaacagc
     7921 aggagtggtc ctgagcacgt cagctatttt agtggcttgt ggaaaaactg ataaagaagc
     7981 agatgcaccg acaacatttt cttatgtcta tgcagtagat ccagcatcat ttggctacag
     8041 tatagcgact cgaacatcga ggacagacgt tattggaaat gttattgatg gtttgatgga
     8101 aaatgataaa tacggcaatg ttgctccttc tcaaaaagac tatgatttga acagtacagg
     8161 atgggctcca agctatcaag atccagcgtc ttacttgaat attatggatc caaaatctgg
     8221 ttctgccatg aaacaccttg gcattacgaa aggaaaagat aaggatgttg tagctaaacc
     8281 tggtttggat aaatataaga aattgttaga agatgctgtt tctgagatca ctgacctaga
     8341 gaagagatat gaaaaatatg ccaaagctca agcttggtcg acagatagtt cattattgat
     8401 gccaacagct tcatctggtg gttttccagt tgtaagtaac gtagtaccat tctcaaaacc
     8461 atactcacaa gttggtatta agggggaacc atatatcttt aaaggaatga aattgcaaaa
     8521 agatattgtt acaacaaaag aatataacga ggtttttaaa aaatggcaaa aagaaaaatt
     8581 ggaatccaat agcaaatacc aaaaagaact agaaaaatac attaaataag gaatggtatt
     8641 gatcttgata aaatttttaa aatactgtca ttttgaatat aaaggagttt gatatggagt
     8701 ggattagatt aataggaata gcaatcattg ttgtgggttt tattttaaaa tttgatacaa
     8761 ttgcaacagt agtcttagct ggtttggtta cagctttagt ttcaggtgtt tctctcgttg
     8821 aatttttgga gattttggga aaagaattta gcaatcagcg agtgctcacg atttttatgg
     8881 ttaccttgcc tcttgtgggg ctgtcagaaa cctttggact caagcaacga tcaatcgatt
     8941 tgattcgaaa gattaaaggt ctgacagttg gaaacttcta tacagtttat ttctttattc
     9001 gagagttagc tggtttcttt tcaattcgtc taggaggaca ccctcagttt gtcagacctt
     9061 tggttcagcc tatgggagaa gcagctgcag agtctcaatt aggtagaaag ttaacagagg
     9121 ttgaagatga gacaataaaa gcgcgtgcgg ctgcgaatga aaattttgga aatttctttg
     9181 ctcaaaatac gtttgtaggt gctgggggag tcctcttgat agggggaaca ttagatcagt
     9241 taggctatga aagcaattat gcagggattg cttctacatc tattattgtt gctgttataa
     9301 cacttattgt agtggggatt tacaattatt tatttgataa aaaattgata tcaaaaaaga
     9361 ctaggggagg agaacaaaaa tgacagagtt ggcaaagcaa ctattagagt tgacctatat
     9421 tgtgattggt tgtcaatttc tccatacagc ctattgtagt tataaagata aaacaaaccc
     9481 agttcgactt gggacatctg cattttggac tctattgtct attacgttta taggtggttc
     9541 ctatatgcca aatatgagta ttggtattat tgtaatccta ttatcgctgt taacattgtt
     9601 taagcaagtc cgtatcggaa ccttgccatc cttagatgaa atgaaagcca atattgaatc
     9661 taacaggttg aaaaataaaa tttttattcc agttatgctg atggcaatac ttgcgttggt
     9721 cttagcgcaa atgattccag aatttagcaa gatttcgatt agccttgccg ccttgtttgc
     9781 tacaatttct gttcttgtga ttaccaatag tcaccctaag agtctgttat cagaaaataa
     9841 tcgaatgact cagcaagttt caacaagtgg tattgttcct caattattag gggctttggg
     9901 ggctattttt actgtagcag gtgttggtga tgttatctct catctgatta gcggtattgt
     9961 tccttcagat agtcgcttta taggagtttt ggcctatgtt cttggaatgg ttctattcac
    10021 aatgattatg ggaaatgctt ttgcagcatt caccgttatt acagcaggtg ttggagttcc
    10081 ctttgtattt gctctgggag ctaatccaat tgtggctggt gctcttgcca tgacagcagg
    10141 ttattgtggg accttattga ccccaatggc tgctaatttt aacgctctac cagcagcatt
    10201 gatggatatg aaagatcaga atggcgttat aaaggctcaa gcaggtgttg ctctagtaat
    10261 gattgttatt cacatattct taatgtactt tctcgcattt tagtaaagga aaataaaatg
    10321 aaaatattgg ttacaggttt taatcctttt ggaggtgaaa agattaatcc agctttggag
    10381 gctgtaaaat tattaccatc tgagattaat ggggctgaag ttcgctgggt agaaattcca
    10441 acggtttttt ataagtcgtc agaagtttta gaggcagaaa tattacgata tcaaccagat
    10501 gctgtacttt gtattggaca agcaggcggc aggaccggct taacacctga acgagtagct
    10561 attaatcaag atgatgctcg catacctgat aacgaaggca atcaaccaat tgatacaccg
    10621 attcgtattg atggagcatc ggcctatttt agtagtttac ctatcaaagc gatggtacaa
    10681 gctatcaaaa aacagggact tccggcagtt gtatccaata gtgcaggaac ctttgtttgc
    10741 aatcatttga tgtaccaagc tctttattta gtggataaaa aattccctaa tatgagagca
    10801 ggtttcatgc atattccata tatgatggaa caagtagtaa ataagccgaa tactgcagga
    10861 atgagtctat gtgatattgt aagaggcata gaggtagcta ttgaagctat tgtagactac
    10921 aaagataagg atttgcagtt agtaggcggt gagactcact gataacctaa aaaggatagt
    10981 caattatgct tgtttactaa ctattaacta tgctaaatca attgaggttg tttacataaa
    11041 actctatatc agagaagcct gatatagagt tttttcttgc tagttttagg attttcttgt
    11101 aaaatagaaa aagtgaagag aggtatgaaa tgagcaagaa agataaaaaa atcgaaattc
    11161 aagtagcgga tgccaaagtt aatgttggta aagacagttt tgaaggttat acattgacta
    11221 tcggtaaaaa agttatcgga gaaattgccg aattagacgg acaatttgcc attataaaga
    11281 atgggaatgt cgatagtttt tataaaaaat tggaaaaagc tgtg
//
LOCUS       DAVLVP010000057        10957 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.057, whole genome shotgun sequence.
ACCESSION   DAVLVP010000057 DAVLVP010000000
VERSION     DAVLVP010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.057"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            14..736
                     /locus_tag="VYN69_001430"
     CDS             14..736
                     /locus_tag="VYN69_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEV1533685.1"
                     /translation="MIRIENLSVSYKETLALKDISLVLHGPTITGIIGPNGAGKSTLL
                     KGMLGIIPHQGQAFLDDKEVKKSLHRIAYVEQKINIDYNFPIKVKECVSLGLFPSIPL
                     FRSLKTKHWKKVQEALEIVGLADYAERQISQLSGGQFQRVLIARCLVQEADYILLDEP
                     FAGIDSVSEEIIMNTLRDLKKAGKTVLIVHHDLSKIPHYFDQVLLVNREVIAFGPTKE
                     TFTETNLKEAYGNQLFFNGGDL"
     gene            733..1581
                     /locus_tag="VYN69_001431"
     CDS             733..1581
                     /locus_tag="VYN69_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000559784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEV1533686.1"
                     /translation="MIAEFIDGLQKFHFLQNALITAIVVGIVAGAVGCFIILRGMSLM
                     GDAISHAVLPGVALSFILGLDFFIGAIVFGLLAAIIITYIKGNSIIKSDTAIGITFSS
                     FLALGIILIGVAKSSTDLFHILFGNILAVQDTDMFITMGVGAAILLLIWIFFKQLLIT
                     SFDELLAKAMGMPVNFYHYLLMVLLTLVSVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKSMIFLSSTFGATASVLGLFIGYSFNVAAGSSIVLTAASFFLISFFIAPKQRYLKLK
                     NKHLLK"
     gene            1613..2542
                     /gene="psaA"
                     /locus_tag="VYN69_001432"
     CDS             1613..2542
                     /gene="psaA"
                     /locus_tag="VYN69_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding
                     lipoprotein/adhesin PsaA"
                     /protein_id="HEV1533687.1"
                     /translation="MKKLGTLLVLFLSAIILVACASGKKDTTSGQKLKVVATNSIIAD
                     ITKNIAGDKIDLHSIVPIGQDPHEYEPLPEDVKKTSEADLIFYNGINLETGGNAWFTK
                     LVENAKKTENKDYFAVSDGVDVIYLEGQNEKGKEDPHAWLNLENGIIFAKNIAKQLSA
                     KDPNNKEFYEKNLKEYTNKLDKLDKESKDKFNKIPAEKKLIVTSEGAFKYFSKAYGVP
                     SAYIWEINTEEEGTPEQIKTLVEKLRQTKVPSLFVESSVDDRPMKTVSQDTNIPIYAQ
                     IFTDSIAEQGKEGDSYYSMMKYNLDKIAEGLAK"
     gene            2665..3156
                     /gene="tpx"
                     /locus_tag="VYN69_001433"
     CDS             2665..3156
                     /gene="tpx"
                     /locus_tag="VYN69_001433"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004245092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HEV1533688.1"
                     /translation="MVTFLGNPVSFTGKQLQVGDKALDFSLTTTDLSKKSLADFDGKK
                     KVLSVVPSIDTGICSTQTRRFNEELAGLDNTVVLTVSMDLPFAQKRWCGAEGLDNAIM
                     LSDYFDHSFGRDYALLINEWHLLARAVFVLDTDNTIRYVEYVDNINSEPNFEAAIAAA
                     KAL"
     gene            complement(3252..5960)
                     /locus_tag="VYN69_001434"
     CDS             complement(3252..5960)
                     /locus_tag="VYN69_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEV1533689.1"
                     /translation="MIKRKTYWKDLIQSFTGSKGRFLSILILMMLGSLALVGLKVTSP
                     NMEATANAYLTTAQTLDLAVMSNYGLDQADQEELKQTEGAEVEFGYLTDVTMDNGQDA
                     IRLYSKPERISTFQLRKGRLPQSDKEIALATHLQGQYSVGQEISFKEKEEGHSSLKDH
                     TYTITGFVDSAEILSQRDMGYAGSGSGTLTAYGVILPSQFDQKVYNIARLKYQDLAGL
                     NAFSSAYEEKSKQHQEELEQILSDNGKVRLQLLKKEGQESLDKGQETLDKAQTNLQEG
                     KRRLAAAQARIQAQESQLALFPQVQREQASAQLTQAKQELGKEEDKLKQAEQNLAQEK
                     EKLEKHQQVLDDLAEPRYQVYNRQTMPGGQGYLMYSNASSSIRAVGNIFPVVLYAVAA
                     MVTFTTMTRFVDEERTHAGIFKALGYRSKDIIAKFLLYGLVAGTVGTALGSILGHYLL
                     ASVISSVITKGMVVGETQIQFYWTYSLLALVLSWLASVLPAYLVAWRELHDEAAQLLL
                     PKPPVKGAKILLERIGFIWRRLSFTHKVTARNIFRYKQRMLMTIFGVAGSVALLFAGL
                     GIQSSVAGVPSKQFQQIQQYQMLVSENPSATNQDKVELAEVLKGQEILAYQKIYSKTL
                     DKDFKGKAGLQNITLMMIEKEDLTPFIHLQHHQQELTLKDGIVITAKLAQLAGVKVGQ
                     TLEIEGKELKVAAITENYVGHFIYMSQASYEQLYGQLPQANTYLVSLRDTSATSIESQ
                     AGLLMSQSAVSSVVQNASAIRLFDSIASSLNQTMTILVIVSVLLAIVILYNLTNINVA
                     ERIRELSTTKVLGFHNNEVTLYIYRETIVLSLVGIVLGLVAGFYLHQFLIQMISPATI
                     LFYPQVGWEVYVIPVAAVSIILTLLGFFVNYYLRKVDMLEALKSVE"
     gene            complement(5962..6663)
                     /locus_tag="VYN69_001435"
     CDS             complement(5962..6663)
                     /locus_tag="VYN69_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533690.1"
                     /translation="MAYIEMKHCYKRYQVGDTEIVANRDVNFEIEKGELVIILGASGA
                     GKSTVLNLLGGMDTNDEGEIWIDGVNIADYSSHQRTNYRRNDVGFVFQFYNLVSNLTA
                     KENVELASEIVTDALNPDQVLTDVGLAHRLNNFPAQLSGGEQQRVSIARAVAKNPKIL
                     LCDEPTGALDYQTGKQVLKILQDMSRQKGATVIIVTHNGALAPIADRVIHMHDASVKD
                     VVLNQHPQDIDSLEY"
     gene            complement(6913..9324)
                     /locus_tag="VYN69_001436"
     CDS             complement(6913..9324)
                     /locus_tag="VYN69_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 95 protein"
                     /protein_id="HEV1533691.1"
                     /translation="MIRNKKQDYVLAYKQPASTTYMGWEEEALPIGNGSLGAKVFGLI
                     GAERIQFNEKSLWSGGPLPDSSDYQGGNLQDQYVFLAEIRQALEKRDYNLAKELAEQH
                     LIGPKTSQYGTYLSFGDIHIEFSQQGTTLSQVTDYQRQLNISKALATTSYVYKGTRFE
                     REAFASFPDDLLVQCFTKEGLETLDFTIELSLTCDLASDGKYEQEKSDYKECKLDITD
                     SHILMKGRVKDNDLRFASYLAWETDGDIRVWSDRVQISGASYANLFLAAKTDFAQNPA
                     SNYRKKLDLEQQVIDLVDTAKEKGYTQLKSRHIEDYQALFQRVQLDLEADVDASTTDD
                     LLKNYKPQEGQVLEELFFQYGRYLLISSSRDCSDALPANLQGVWNAVDNPPWNSDYHL
                     NVNLQMNYWPAYVTNLLETVFPVINYVDDLRVYGRLAAVKYAGIVSQKGEENGWLVHT
                     QATPFGWTAPGWDYYWGWSPAANAWMMQTVYEAYSFYRDQDYLREKIYPMLRETVRFW
                     NAFLHKDQQAQRWVSSPSYSPEHGPISIGNTYDQSLIWQLFHDFIQAAQELGLDEDLL
                     TDVKEKSDLLNPLQITQSGRIREWYEEEEQYFQNEKVEAQHRHASHLVGLYPGNLFSY
                     KGQEYIEAARASLNDRGDGGTGWSKANKINLWARLGDGNRAHKLLAEQLKTSTLPNLW
                     CSHPPFQIDGNFGATSGMVEMLLQSHAAYLVPLAALPDAWSTGSVSGLMARGHFEVSM
                     SWEDKKLLQLTILSRSGGDLRVSYPDIEKSVIKMNQEKIKAKCMGKDCISVATAEGDL
                     VQFYF"
     gene            complement(9377..10069)
                     /locus_tag="VYN69_001437"
     CDS             complement(9377..10069)
                     /locus_tag="VYN69_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006703946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase"
                     /protein_id="HEV1533692.1"
                     /translation="MVKLVFARHGESEWNKANLFTGWADVDLSEKGTQQAIDAGKLIK
                     EAGIEFDQAYTSVLKRAIKTTNLALEASDQLWVPVEKSWRLNERHYGGLTGKNKAEAA
                     EQFGDEQVHIWRRSYDVLPPNMDRDDEHSAHTDRRYASLDDSVIPDAENLKVTLERAL
                     PFWEDKIAPALKDGKNVFVGAHGNSIRALVKHIKGLSDDEIMDVEIPNFPPLVFEFDE
                     KLNVVSEYYLGK"
     gene            complement(10604..10939)
                     /locus_tag="VYN69_001438"
     CDS             complement(10604..10939)
                     /locus_tag="VYN69_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000508814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533693.1"
                     /translation="MGGILLLIGPFVLFGTAVNTAATTLNGGATAGAFSGVALLLNAL
                     KIANLVLGIIAIVYYKGDKRVGAAPSVLMIVSGEVSLIPFLGWVGRILAIIGGSLFLA
                     TLKKFKSEE"
BASE COUNT         3207 a         2522 c         2044 g         3184 t
ORIGIN      
        1 ggaatacaag tttatgatac gtatcgaaaa cctcagtgtc tcctacaaag aaacgttggc
       61 acttaaggat atttcactag tgctccatgg accaacaatt accggcatca ttggtccaaa
      121 cggcgctggg aaatcaacac tattaaaagg tatgctggga attatcccac atcaaggtca
      181 ggcatttctc gatgacaagg aagttaaaaa atccttacac cgaattgcct atgtcgaaca
      241 aaaaatcaat atcgactaca actttcccat caaggtcaag gaatgcgtct cgttaggact
      301 atttccctct attcctctct ttcgaagttt aaagactaaa cattggaaga aagtgcaaga
      361 agcccttgaa atcgtcggcc tagctgacta cgctgaacgt caaattagtc aactgtctgg
      421 aggtcaattc cagcgggtct tgattgccag atgtttggtg caggaagccg actatatcct
      481 cttggatgaa ccctttgctg ggattgactc tgtcagtgag gaaatcatca tgaatacgct
      541 gagagatttg aaaaaagctg ggaagacggt tctcatcgtt caccacgacc tcagcaagat
      601 tccccactac ttcgatcaag tcttacttgt caatcgagaa gtgattgcct ttggtccaac
      661 aaaagaaact tttaccgaaa ccaatctaaa agaagcttac ggtaatcaac tctttttcaa
      721 tggaggtgac ctatgattgc agaatttatc gatggattgc aaaaattcca tttcctacaa
      781 aatgccttga taacagctat tgtcgtcggg attgtagctg gagccgtggg atgtttcatt
      841 attctacgcg ggatgtcact catgggagat gccatttcac atgctgtctt accaggtgta
      901 gccctctcct tcatcttggg ccttgacttc tttatcggag ccattgtctt tggattgcta
      961 gctgccatca tcattaccta catcaaagga aactcgatta tcaaaagcga taccgccatc
     1021 ggcattacct tttcttcttt cttagccctc ggtatcatct tgattggtgt cgctaaaagt
     1081 tcaactgacc ttttccatat cctttttggt aatatcctgg ccgtccaaga tacggatatg
     1141 tttattacta tgggtgtggg ggcagccatc ctcttgttaa tctggatttt cttcaagcaa
     1201 ctcttgataa cttcctttga tgaactcttg gctaaagcca tgggaatgcc tgtcaatttc
     1261 tatcactacc ttctcatggt actcctgact ctcgtgtctg tgacagccat gcaaagtgtc
     1321 ggaactatcc tgattgtagc catgctgatt accccagctg caactgctta tctgtatgct
     1381 aatagtctga aaagcatgat tttcctttcc tcaaccttcg gagctactgc ttcagttttg
     1441 ggactcttta ttggctatag ttttaatgtt gcggcaggtt ctagtatcgt gcttacagct
     1501 gctagtttct ttctcattag cttctttatc gctcccaaac aacgatattt gaaactgaaa
     1561 aataaacatt tgttaaaata aggggcaaag ccctaataaa ttggaggatc taatgaaaaa
     1621 attaggtaca ttactcgttc tctttctttc tgcaatcatt cttgtagcat gtgctagcgg
     1681 aaaaaaagat acaacttctg gtcaaaaact aaaagttgtt gctacaaact caatcatcgc
     1741 tgatattact aaaaatattg ctggtgacaa aattgacctt catagtatcg ttccgattgg
     1801 gcaagaccca cacgaatacg aaccacttcc tgaagacgtt aagaaaactt ctgaggctga
     1861 tttgattttc tataacggta tcaaccttga aacaggtggc aatgcttggt ttacaaaatt
     1921 agtagaaaat gccaagaaaa ctgaaaacaa agactacttc gcagtcagcg acggcgttga
     1981 tgttatctac cttgaaggtc aaaatgaaaa aggaaaagaa gacccacacg cttggcttaa
     2041 ccttgaaaac ggtattattt ttgctaaaaa tatcgccaaa caattgagcg ccaaagaccc
     2101 taacaataaa gaattctatg aaaaaaatct caaagaatat actaataagt tagacaaact
     2161 tgataaagaa agtaaggata aatttaataa gatccctgct gaaaagaaac tcattgtaac
     2221 cagcgaagga gcattcaaat acttctctaa agcctatggt gttccaagtg cctacatctg
     2281 ggaaatcaat actgaagaag aaggaactcc tgaacaaatc aagaccttgg ttgaaaaact
     2341 tcgccaaaca aaagttccat cactctttgt agaatcaagt gtggatgacc gtccaatgaa
     2401 aactgtttct caagacacaa acatcccaat ctacgcacaa atctttactg actctatcgc
     2461 agaacaaggt aaagaaggcg acagctacta cagcatgatg aaatacaacc ttgacaagat
     2521 tgctgaagga ttggcaaaat aagcctctga aaaacgtcat tctcatgtga gctggcgttt
     2581 tttctatgca cacatttccg gtcaaatcat tggaaaattc tgactgtttc agatacaatg
     2641 gaagaaaaaa gattggagta tcctatggta acttttctcg gaaatcctgt gagctttaca
     2701 ggtaaacaac tacaagtcgg cgacaaggcg cttgattttt ctcttactac aacagatctt
     2761 tctaaaaaat ctctggctga ttttgatggc aagaaaaaag tcttgagtgt cgttccttct
     2821 atcgatacag gcatctgctc aactcaaaca cgtcgtttta atgaagaatt ggctggactg
     2881 gacaacacgg tcgtattgac tgtttcaatg gacctacctt ttgctcaaaa acgttggtgc
     2941 ggtgctgaag gccttgacaa tgccattatg ctttcagact actttgacca ttctttcggg
     3001 cgcgattatg ccctcttgat caacgaatgg cacctattag cacgcgcagt ctttgtcctc
     3061 gatactgaca atacgattcg ctacgttgaa tacgtggata atatcaattc tgagccaaac
     3121 ttcgaagccg caattgcagc tgctaaagcc ctatagaaaa aatcctctgt cacaaagagt
     3181 tccctgctct ttgaaacgga ggatttttga atattagtaa atagaagttc aatcagctaa
     3241 aaataactac cttactctac agatttcagg gcttctaaca tgtcaacctt tctcagataa
     3301 taattgacga agaaaccaag caaggtcaaa atgatgctta ctgctgccac tgggattaca
     3361 tagacttccc agcctacctg cggataaaag agaatagtcg caggcgaaat catttgaatc
     3421 aaaaattggt gtaaatagaa accagctacc agaccaagta cgattcccac aagggatagc
     3481 acaatcgtct cacggtaaat gtagagggtg acttcattat tatgaaaacc aagaaccttg
     3541 gtagtggaga gttcacggat tctctcagct acgttgatat tggtcagatt gtaaaggatg
     3601 acaatagcta atagaaccga tacgatgacc aagatggtca tggtctgatt gagtgagcta
     3661 gcgatagagt cgaagagtcg aatggctgaa gcattttgga caacgctgga caccgcagat
     3721 tgactcataa gcaagcccgc ctgactttcg atactagttg cactggtatc ccttaatgag
     3781 accagataag tgttggcttg gggtagctgt ccgtaaagtt gctcatagct agcctgactc
     3841 atataaataa agtgaccaac gtagttctca gtaatagcag cgacctttag ttccttacct
     3901 tcaatttcta aagtctgccc aaccttgaca cctgccagct gggcgagttt agctgtaata
     3961 acgatgccat cttttaatgt cagctcctgc tgatgatgtt gaagatggat aaagggagtc
     4021 aaatcttcct tctctatcat cataagagta atgttttgaa gaccagcctt gcctttgaaa
     4081 tccttgtcta gcgttttaga atagattttc tggtaggcta gtatctcctg ccctttcaac
     4141 acttctgcta gctctacctt gtcctgattg gtcgcactag gattttcaga gacaagcatc
     4201 tgatactgtt ggatttgttg aaactgttta gacggaactc ctgctacaga agattggatt
     4261 cccaaacctg caaagagcag agctacagaa cctgccacac caaagattgt catcaacatt
     4321 ctctgcttat aacgaaagat gttgcgggct gttaccttat gagtaaaact gagacgacgc
     4381 cagataaaac cgatacgctc caataagatt ttagctcctt tgacaggagg tttaggaagt
     4441 agaagctggg ctgcttcgtc atgaagttcc ctccaagcca ccagataggc tggtaacaca
     4501 ctcgccaacc agctcaagac aagagctagt aagctatagg tccaatagaa ctgaatctgg
     4561 gtttctccca ccaccatgcc ttttgtaatg acacttgaaa ttacactggc tagcaaataa
     4621 tgaccaagta tactacctag agccgttccg acagtcccag ctactagtcc ataaaggaga
     4681 aacttggcga taatatcctt actacggtaa cccaaggcct taaaaatccc tgcatgagtt
     4741 cgctcttcgt ctacaaagcg agtcatggtc gtaaaggtca ccatggctgc tacggcataa
     4801 agtaccacag gaaagatatt gcccactgct cgaatactgg atgaagcatt gctatacata
     4861 agatagccct gaccacctgg catggtctga cgattataga cctgatacct tggctccgcc
     4921 aaatcgtcca agacttgctg atgtttttct aatttttcct tttcttgggc tagattttgt
     4981 tcagcttgct ttagtttgtc ctcttccttg cccaattcct gcttggcttg ggtaagttga
     5041 gcactagcct gctctctctg aacttgagga aacaaggcta gttgactttc ttgagcctgt
     5101 atacgagctt gagcagctgc taaacgacgc ttgccttcct gcaaattagt ctgagccttg
     5161 tcaagggtct cttgcccctt gtctagagac tcttgtcctt cttttttcaa gagttgcaga
     5221 cgtaccttgc cattatctga taaaatttgt tcaagctctt cctgatgttg cttagatttt
     5281 tcttcataag ctgatgaaaa ggcatttaaa cctgctaaat cttgatattt caaacgagct
     5341 atattgtaga ctttctgatc aaattgacta ggtaaaatca ccccataggc tgtcagagtc
     5401 ccacttccac ttcctgcgta gcccatatct cgctgggaga ggatttcagc cgaatccaca
     5461 aaaccagtaa tggtataagt atggtctttt aaagaggaat gaccctcttc tttttcttta
     5521 aaactaatct cctgtcccac gctgtattgg ccttgcaaat gagtggccaa agcgatttcc
     5581 ttgtctgact gaggaagtcg tccctttctt agctgaaagg ttgaaattcg ctctggtttg
     5641 gagtacagcc gaatggcatc ctgcccatta tccatagtca catctgtcaa atagccaaac
     5701 tcgacctctg cgccctccgt ctgttttagt tcttcttggt ctgcttgatc caagccatag
     5761 ttagacatga ctgccaaatc caaggtttga gcagttgtta aataagcatt agctgtcgcc
     5821 tccatattgg gactggttac tttgaggcct actaaggcta gagatcccaa catcatcagg
     5881 atcaagatgg ataaaaaacg ccccttggag cctgtgaagg actgaattaa gtccttccaa
     5941 taagtttttc gcttgatcat gctagtactc caaactgtca atatcctgag gatgctggtt
     6001 gagcaccaca tccttgacac tggcatcgtg catatgaatc acgcgatcag caatgggcgc
     6061 caaagctcca ttatgagtca cgatgatcac cgtcgctccc ttttgacgag acatatcttg
     6121 gagaattttc aaaacctgct tgcccgtctg ataatccaag gctccagtag gttcatcaca
     6181 aagaagaatt ttaggatttt tggctaccgc gcgtgcaatg gagactcgct gttgctcccc
     6241 tccagaaagc tgggctggaa agttatttag acgatgagcc agacctacat ctgtcaagac
     6301 ctgatcagga ttcaaggcat ctgtcacaat ttcagaagcc agttccacat tttccttagc
     6361 tgtcagatta gaaactagat tataaaactg aaaaacaaac cccacatcat ttctacggta
     6421 attggtgcgc tggtgggaac tataatccgc aatattaaca ccatcaatcc agatttcccc
     6481 ttcatcattg gtatccattc ccccaagaag gttgagaact gttgacttgc ctgcacctga
     6541 agcaccaagg ataataacca gctccccctt ttcaatctca aaattcacat cacgattagc
     6601 cacaatctcc gtgtccccaa cctgataacg cttgtaacag tgtttcatct caatataagc
     6661 catcagaccc accctctatc aaacaaatta tactcctcga aaatctctct tcaaaccacg
     6721 tcagcttcca tctgcaacct caaaacactg ttttgagcaa cctgcggcta gcttcctagt
     6781 ttgtgctctt tgattttcat taagtattac ttcctattat cattataacg ctttttcaac
     6841 tagttgaaaa ctgcttatat tcttaaatcc ttattaaaag gcagtttcaa attactgcct
     6901 tataacatct tcttaaaaat aaaattgaac aagatcacct tctgctgttg ccaccgaaat
     6961 acaatctttc cccatgcatt tcgcttttat tttttcttga ttcattttaa tcacactctt
     7021 ctcaatatct ggataagaaa ctcgcaaatc tcctccactc cttgataaaa tggtcaactg
     7081 taagagtttt ttatcttccc agctcatgct cacttcaaaa tgtccacgtg ccattaagcc
     7141 tgaaacagaa cctgttgacc aagcatcagg tagggcagct agaggtacca gataagctgc
     7201 atgagactgg agtaacattt ctaccatgcc actagtagca ccaaaattac catctatctg
     7261 aaaaggagga tggctacacc aaagatttgg caaggtggat gtctttaact gctctgccaa
     7321 taatttatgg gctcgattgc catctcccaa acgcgcccag agattgatct tattagcctt
     7381 ggaccagcct gtgccgccat ctccacgatc attgaggcta gcacgcgccg cttcaatata
     7441 ctcttgtccc ttgtagctaa agagattgcc aggatagagt cccactagat gggaagcatg
     7501 ccgatgctgg gcctccactt tctcattttg aaaatactgc tcttcctcct cataccactc
     7561 cctgattcga ccagattgag tgatttgcaa aggatttagt aaatcagact tctccttaac
     7621 atcagtcaac aagtcctcat ccagtcccaa ttcctgagca gcctgaataa aatcatgaaa
     7681 taactgccaa atcagagatt ggtcataagt attgccaatc gaaatcggcc catgttctgg
     7741 ggaataagac ggagaagaca cccaacgctg cgcctgctga tccttatgta aaaaggcatt
     7801 ccaaaaacga accgtttccc tcaacatggg ataaattttc tccctgagat agtcttggtc
     7861 cctataaaat gaataggctt cataaacggt ttgcatcatc cacgcattgg cagctggtga
     7921 ccaaccccaa tagtaatccc aaccaggtgc cgtccaacca aagggagtcg cttgagtatg
     7981 aaccaaccaa ccattctcct cacctttctg agagacgatt cctgcatact ttacagccgc
     8041 tagacgacca tagacacgca aatcatctac atagttgatg actggaaaga ccgtctctag
     8101 gagattggta acataggctg gccaataatt catctgcaga ttgacattta agtgatagtc
     8161 cgaattccaa ggaggattgt cgaccgcatt ccagactccc tgtaggttag ctggtagagc
     8221 atctgagcag tctctggacg aactaatcaa taaataccgt ccatactgga agaacaactc
     8281 ctccaaaacc tgcccctctt gtggcttata attttttaac aaatcatctg tagtggatgc
     8341 gtcaacatca gcttccaaat ccaattgaac acgctggaat aaggcttggt agtcctcgat
     8401 atgccttgat ttcaattggg tatagccctt ttctttagct gtgtccacca agtctatcac
     8461 ctgttgctct aaatctagtt tcttgcgata attgctagca ggattttggg caaaatccgt
     8521 cttagcggcc aagaagagat tggcataact ggctcctgat atctgaaccc tatctgacca
     8581 aactctaata tctccatccg tttcccaagc tagataacta gcaaaccgca gatcattatc
     8641 cttaactctt cccttcatca agatatgaga atcagtaata tccaacttac actccttgta
     8701 atcagatttt tcctgctcat actttccatc agaagccaaa tcacaggtca aggatagttc
     8761 tatagtaaaa tctagagttt ccaacccttc cttagtaaaa cattgaacca agagatcatc
     8821 tggaaaactc gcaaaagctt cacgttcaaa tcgcgttccc ttatagacat aagaagtcgt
     8881 cgcaagtgcc ttactaatat tcagctgtct ctgatagtcc gtcacctgag acaaagtcgt
     8941 accttgctgg ctgaactcaa tgtgaatatc cccaaaagac agataggtcc catattgact
     9001 cgtttttggc ccaattaggt gctgctcagc cagttcctta gccagattgt aatctctctt
     9061 ctccaaagcc tgccgaatct cagctaaaaa aacatactga tcctgaagat ttccaccctg
     9121 ataatctgaa ctatcaggaa gtggacctcc agaccagaga cttttttcat taaattgaat
     9181 ccgttcagcc cctataaggc caaatacttt tgctcctaaa gaaccattac ctatcggtaa
     9241 agcttcttct tcccaaccca tgtaagtggt tgaagctggt tgcttgtagg ccagtacata
     9301 atcttgtttt ttattcctta tcatacttcc atactaacat aaaaagccta gaaatcaatc
     9361 ctaggcttac aattttttat tttccaaggt agtattcaga aacaacgttc aatttttcat
     9421 cgaattcgaa taccaatggt gggaagttag ggatttccac gtccatgatc tcatcatctg
     9481 acaaaccttt gatgtgtttt acaagggcac ggattgagtt accgtgagct cctacgaata
     9541 cgtttttacc atctttaaga gctggagcga ttttatcttc ccagaatgga agagcacgtt
     9601 ccaaagtcac tttcaagttt tcagcatctg ggattactga gtcgtcaagt gaagcataac
     9661 gacggtctgt gtgagctgag tgctcatcat cacggtccat atttggaggc aatacatcgt
     9721 atgaacgacg ccagatgtga acttgctcat caccaaattg ttcagcagct tcagctttgt
     9781 ttttaccagt caaaccaccg tagtgacgtt cgttcaagcg ccatgatttt tcaactggaa
     9841 cccataattg gtcagaagct tcaagagcca agttagttgt tttgatagca cgtttcaata
     9901 ctgaagtgta agcttggtca aattcgatac cagcttcttt gatcaattta ccagcgtcaa
     9961 tcgcttgttg tgtacctttt tcagacaaat caacatcagc ccaaccagtg aaaaggttag
    10021 ctttgttcca ttcagactca ccgtggcgag caaaaaccaa ttttaccatt agatggattc
    10081 tcctttaaat tttccgaggt ttcccctcgt ttatctattc tattttacac aatttttcac
    10141 aaaaaagcta gtcaaaacca aaaaggaaag gactgtttga caatgtttgg caatcacatt
    10201 gtttttggtt gtttctattc tatcttcatt atttaacctt tttttctgcg catcgaataa
    10261 tttttgagtt gactctttta gtttagtaaa cgagtcatta ttttccatag ctgtgccttt
    10321 gaaaccttcc tcagctttag taaaataatc atcaaaaata tttttataaa gatctatata
    10381 atctgcataa gtgcgtattg attcaatcgc atcgtctgtt acaaccggtg tatcgaaagt
    10441 gattgtttct tttgaactat tggatgttgc tactgtcttc tcatcattac ttgttgaagt
    10501 attctttgaa ctttatgttc cacatgcaac tagaagagaa agagataata gagctactcc
    10561 tataccgata aacttttttg ttcttttcat gctaaaatac cttttattct tctgatttga
    10621 atttcttcaa tgttgcaagg aatagagatc ctccgataat agcaagaatc ctcccaaccc
    10681 atcctaagaa tggaatgaga ctaacttcac cagaaacaat cattagtaca gacggagctg
    10741 cacctacacg cttatctcct ttatagtaaa caatagcaat gataccaaga acaagatttg
    10801 caatcttcaa ggcattcaag agtaaggcaa cacctgaaaa agcccctgca gtagctcctc
    10861 cattaagagt tgtagcagct gtatttaccg ctgttccaaa caagacaaaa ggaccaataa
    10921 gaagtaaaat tcctcccaca agaccgacga taccact
//
LOCUS       DAVLVP010000058        10702 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.058, whole genome shotgun sequence.
ACCESSION   DAVLVP010000058 DAVLVP010000000
VERSION     DAVLVP010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10702
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.058"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            105..602
                     /locus_tag="VYN69_001439"
     CDS             105..602
                     /locus_tag="VYN69_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000920162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEV1533694.1"
                     /translation="MLKSEKQSRYQMLNEELSFLLEGETNVLANLSNASALIKSRFPN
                     TVFAGFYLFDGKELVLGPFQGGVSCIRIALGKGVCGEAAHFQETVIVGDVTTYLNYIS
                     CDSLAKSEIVVPMMKNGQLLGVLDLDSSEIEDYDAMDRDYLEQFVAILLEKTAWDFTM
                     FEEKS"
     gene            602..2257
                     /gene="dnaX"
                     /locus_tag="VYN69_001440"
     CDS             602..2257
                     /gene="dnaX"
                     /locus_tag="VYN69_001440"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEV1533695.1"
                     /translation="MYQALYRKYRSQNFSQLVGQEVVAKTLKQAVEQEKISHAYLFSG
                     PRGTGKTSVAKIFAKAMNCPNQVGGEPCNNCYICQAVTDGSLEDVIEMDAASNNGVDE
                     IREIRDKSTYAPSLARYKVYIIDEVHMLSTGAFNALLKTLEEPTQNVVFILATTELHK
                     IPATILSRVQRFEFKSIKTQDIKEHIHYILEKENISSEPEAVEIIARRAEGGMRDALS
                     ILDQALSLTQGNELTTAISEEITGTISLSALDDYVAALSQQDVPKALSCLNLLFDNGK
                     SMTRFVTDLLHYLRDLLIVQTGGENTHHSSVFVENLALPQKNLFEMIRLATVSLADIK
                     SSLQPKIYAEMMTVRLAEIKPEPALSGAVENEIATLRQEVARLKQELSNAGAVPKQVA
                     PAPSRPATGKTVYRVDRNKVQSILQEAVENPDLARQNLIRLQNAWGEVIESLGGPDKA
                     LLVGSQPVAANEHHAILAFESNFNAGQTMKRDNLNTMFGNILSQAAGFSPEILAISME
                     EWKEVRAAFSAKAKSSQTEKEVEESLIPEGFEFLADKVKVEED"
     gene            2285..2479
                     /locus_tag="VYN69_001441"
     CDS             2285..2479
                     /locus_tag="VYN69_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3272 domain-containing protein"
                     /protein_id="HEV1533696.1"
                     /translation="MNKQQFIIMALFTAAETYFFNEAWMTGRYIMAAFWAILLFRNFR
                     VSYVMGKIVDVIDQHFNRKD"
     gene            2629..3399
                     /gene="sufC"
                     /locus_tag="VYN69_001442"
     CDS             2629..3399
                     /gene="sufC"
                     /locus_tag="VYN69_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEV1533697.1"
                     /translation="MSVIEIKDLHVEIEGKEILKGVNLTLKTGEIAAIMGPNGTGKST
                     LSAAIMGNPNYEVTKGEVLFDGVNILELEVDERARMGLFLAMQYPSEIPGITNAEFLR
                     AAMNAGKEDDEKISVREFITKLDEKMELLNMKEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFALLDEIDSGLDIDALKVVSKGVNAMRGEGFGAMIITHYQRLLNYITPDVVHV
                     MMEGRVVLSGGPELAARLEREGYAKLAEELGYDYKEEL"
     gene            3427..4689
                     /gene="sufD"
                     /locus_tag="VYN69_001443"
     CDS             3427..4689
                     /gene="sufD"
                     /locus_tag="VYN69_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEV1533698.1"
                     /translation="MTRENIKLFSEMHAEPSWLADLRQKAFDKIETLELPVIERVKFH
                     RWNLGDGTITENEPSANVPDFTALDHHLKLVQVGTQTVFEQTPVELAEQGVVFTDFHS
                     ALEEIPELIEEFFMSSVKYDDDKLAAYHTAYFNSGAVLYIPDNVEITEPIEGIFYQDS
                     DSNVPFNKHIMIIAGKNSKINYLERLESRGEGSAKATANITVEVIARSGAQVKFAAID
                     RLGENVTAYISRRGKLGNDASIDWAIGVMNEGNVVADFDSDLIGNGSHADLKVVALSS
                     GRQVQGIDTRVTNYGCNSIGNILQHGVILEKATLTFNGIGHIIKGAKGADAQQESRVL
                     MLSDQARSDANPILLIDENDVTAGHAASIGQVDPEDMYYLMSRGLDKATAERLVVRGF
                     LGSVIVEIPVKEVRDEMIATIEEKLSKR"
     gene            4700..5926
                     /locus_tag="VYN69_001444"
     CDS             4700..5926
                     /locus_tag="VYN69_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEV1533699.1"
                     /translation="MLDVEAIRKDFPILDQIVNDEPLVYLDNAATTQKPLVVLKVINS
                     YYEQDNANVHRGVHTLAERATASYEAARETIRKFINAGSTKEVLFTRGTTTSLNWVAR
                     FAEEILTEGDQVLISVMEHHSNIIPWQEACRKTGAELVYVYLKDGALDMEDLRAKLTD
                     KVKFVSLAHASNVLGVVNPIKEITQLAHQVGAIMVVDGAQSTPHMKIDVQDLDLDFFA
                     FSGHKMAGPTGIGVLYGKEKYLEQMSPVEFGGEMIDFVYEQFASWKELPWKFEAGTPN
                     MAGAIGLATAVDYLEKIGMDAVEAHEQELIAYVYPKLQAIEGLTIYGSQDLAQRSGVI
                     AFNLGDLHPHDLATALDYEGVAVRAGHHCAQPLLQYLEVPATARASFYIYNTKADCDK
                     LVDALQKTKEFFNGTF"
     gene            5913..6353
                     /locus_tag="VYN69_001445"
     CDS             5913..6353
                     /locus_tag="VYN69_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEV1533700.1"
                     /translation="MALSKLDSLYMAVVADHSKNPHHQGKLEDAEQISLNNPTCGDVI
                     NLSVKFDAEDRLEDIAFLNSGCTISTASASMMTDAVLGKTKQEILELATIFSEMVQGQ
                     KDERQGQLGDAAFLSGVAKFPQRIKCATLAWNALKKTIENQEKQ"
     gene            6407..7819
                     /gene="sufB"
                     /locus_tag="VYN69_001446"
     CDS             6407..7819
                     /gene="sufB"
                     /locus_tag="VYN69_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEV1533701.1"
                     /translation="MAEERVEPKPIDLGEYKFGFHDDVEPVLSTGKGLNEGVIRELSA
                     AKGEPEWMLEFRLKSYETFKKMPMQTWGADLSEIDFDDLIYYQKPSDKPARSWDDVPE
                     KIKETFERIGIPEAERAYLAGASAQYESEVVYHNMKEEFQKLGIIFTDTDSALKEYPD
                     LFKQYFAKLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDIPLQTYFRINNENIGQFE
                     RTLIIVDEGASVHYVEGCTAPTYSSNSLHAAIVEIFALDGAYMRYTTIQNWSDNVYNL
                     VTKRAKAQKDATVEWIDGNLGAKTTMKYPSVYLDGEGARGTMLSIAFANAGQHQDTGA
                     KMIHNAPHTSSSIVSKSIAKGGGKVDYRGQVTFNKNSKKSVSHIECDTIIMDDLSASD
                     TIPFNEIHNSQVALEHEAKVSKISEEQLYYLMSRGLSESEATEMIVMGFVEPFTKELP
                     MEYAVELNRLISYEMEGSVG"
     gene            complement(8050..9291)
                     /locus_tag="VYN69_001447"
     CDS             complement(8050..9291)
                     /locus_tag="VYN69_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEV1533702.1"
                     /translation="MKKIFLTLLTVSLLGGASTAVAQDFTIAAKHAIAVEANTGKILY
                     EKDATQPVEIASITKLITVYLVYEALENGSITLSTPVDISDYPYQLTTNSEASNIPME
                     ARNYTVEELLEATLVSSANSAAIALAEKIAGSEKDFVDMMRAKLLEWGIQDATVVNTT
                     GLNNETLGDNIYPGSKKDEENKLSAYDVAIVARNLIKKYPQVLEITKKPSSTFAGMTI
                     TSTNYMLEGMPAYRGGFDGLKTGTTDKAGESFVGTTVEKGMRVITVVLNADHQDNNPY
                     ARFTATSSLMDYISSTFTLRKIVQQGDAYQDSKAPVQDGKEDTVTAVAPEDIYLIERV
                     GNQSSQSVQFTPDSKAIPAPLEAGTVVGHLTYEDKDLIGQGYITTERPSFEMVADKKI
                     EKAFFLKVWWNQFVRFVNEKL"
     gene            9394..10503
                     /locus_tag="VYN69_001448"
     CDS             9394..10503
                     /locus_tag="VYN69_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000489839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEV1533703.1"
                     /translation="MFRRNKLFFWTTEILLLTIIFYLWRQMGSLINPFVSVLNTIMIP
                     FLLGGFLYYLTNPIVTFLNKVCKLNRLLGILITLCTLVWGMVIGVVYLLPILINQLSS
                     LIISSQTIYSRVQDLIIDLSNYPALQNLDVEATIQQLNLSYVDILQNILNSVSNSVGS
                     VLSALISTVLILIMTPVFLVYFLLDGHKFLPMLERTILKRDRLHIAGLLKNLNATIAR
                     YISGVSIDAIIIGCLAYIGYSIIGLKYALVFAIFSGVANLIPYVGPSIGLIPMIIANI
                     FTDPHRLLIAVIYMLVVQQVDGNILYPRIVGSVMKVHPITILVLLLLSSNIYGVVGMI
                     VAVPTYSILKEISKFLSRLYENHKIMKERERELAK"
BASE COUNT         3180 a         1955 c         2325 g         3242 t
ORIGIN      
        1 ataggggatt tacccactac aaatattata gagcccaaaa tcctttgttt actaaacaag
       61 ggatttttct tttgtctctg ctcctttttt gatataatag ttttatgtta aaatcagaaa
      121 aacaatcacg ttatcaaatg ttaaatgaag aattgtcctt cctattggaa ggcgaaacca
      181 atgttttggc taatctttcc aacgccagtg ctctcataaa atcacgtttt cctaataccg
      241 tatttgcagg cttttatttg ttcgatggaa aggaattggt tttaggtccc ttccaaggag
      301 gtgtttcctg catccgtatt gcactaggca agggtgtttg tggtgaggca gctcactttc
      361 aggaaactgt tattgttgga gatgtgacga cctatctcaa ctatatttct tgtgatagtc
      421 tagctaaaag tgaaattgtg gtgccgatga tgaagaatgg tcagttactt ggagttctgg
      481 atctggattc ttcagagatt gaggattacg atgctatgga tcgagattat ttggaacaat
      541 ttgtcgctat tttgcttgaa aagacagcat gggactttac gatgtttgag gaaaaatctt
      601 aatgtatcaa gcactttatc gaaaatatag aagtcaaaac ttctcccagt tagttggtca
      661 agaagttgtg gctaagactc ttaaacaagc ggtggagcaa gagaaaataa gtcacgctta
      721 tcttttttct ggtcctcgtg gaacgggaaa aaccagtgtt gctaaaatct ttgccaaggc
      781 tatgaactgt cccaatcaag tgggtggcga accttgcaat aactgctata tttgtcaagc
      841 agtgacggac ggtagtttag aagatgtcat tgaaatggat gcagcttcta ataatggggt
      901 agatgaaatt cgcgaaattc gtgataaatc tacctatgcg cctagccttg ctcgttataa
      961 ggtttatatc atagatgagg ttcacatgct gtctacaggg gcttttaatg ccctcctaaa
     1021 gacgctggaa gaaccaacac agaatgtagt ctttattttg gccactactg aattgcacaa
     1081 gattcctgct actattctat cccgtgtgca acgttttgag tttaaatcaa ttaagacaca
     1141 ggatattaag gaacatattc actatatctt agaaaaagaa aatatcagtt ctgaaccaga
     1201 ggctgtggaa atcattgcca gacgggcgga aggtggaatg cgggacgcct tgtctatttt
     1261 ggatcaagcc ctgagtttga cacagggaaa tgagctgacg actgctatct ctgaagaaat
     1321 tactggcacc attagcctat cagccttgga tgattatgtg gcggccttgt ctcaacagga
     1381 tgttcccaaa gctttgtctt gcttgaatct tctttttgac aatggtaaga gcatgactcg
     1441 ttttgtgacc gatcttttgc actatttaag agacttgtta attgttcaaa cagggggaga
     1501 aaatactcat catagttcag tctttgtaga aaatttggca cttcctcaaa aaaatctgtt
     1561 tgaaatgatt cgcttagcaa cagtgagttt agcagatatt aagtctagtt tgcagcccaa
     1621 gatttatgct gaaatgatga ccgtccgttt ggcggaaatc aagcccgaac cagctctatc
     1681 aggagcggtt gaaaatgaaa ttgctacgct gagacaggaa gttgcccgtc tcaaacaaga
     1741 gctttctaat gcaggtgcgg ttcctaaaca agttgcacca gctcctagtc gaccagctac
     1801 gggcaaaaca gtctatcgtg tcgatcgcaa taaagtgcaa tctatcttac aagaggccgt
     1861 cgaaaatcct gatttagcac gtcaaaatct aattcgtttg cagaatgcct ggggagaggt
     1921 aattgaaagt ctaggtgggc cggacaaggc tctgctagtt ggttctcaac cggttgctgc
     1981 caatgaacac catgctattc ttgcttttga gtctaacttc aatgctggtc aaactatgaa
     2041 acgagacaat ctcaatacca tgtttggtaa tatcctcagt caggcggcag gtttttcacc
     2101 tgagatttta gctatttcca tggaggaatg gaaagaagtt cgcgcagcct tttcagccaa
     2161 agccaaatct tctcaaactg aaaaagaagt agaagaaagc ctgattccag aaggatttga
     2221 atttttggct gataaagtga aggtagagga agactaaaga aagatttcat gatacaataa
     2281 gtttatgaat aaacaacaat ttattattat ggcgctattt acagctgctg agacctattt
     2341 tttcaatgaa gcctggatga ctggccgcta tattatggca gccttttggg caattttact
     2401 ctttagaaat ttccgagtca gttatgtgat gggtaaaatc gttgatgtca tcgatcagca
     2461 ttttaatagg aaagattagc cctcagcttc cagacaaaat caaagccttt taggcttttt
     2521 tttgttatac tagaaaagta tatttataga atttttgctc tatttctggg gaaatcagac
     2581 gtttttctag taagtactgt aaaagttttg aaaaagaaag gaactatcat gtcagtaata
     2641 gagatcaaag atcttcacgt tgagattgaa ggaaaagaaa ttttaaaagg ggttaacctg
     2701 accctgaaaa caggagaaat tgccgctatc atgggaccaa atggtacagg taaatcgact
     2761 ctttctgccg ctatcatggg aaatccaaac tatgaagtaa ctaaaggtga agttttgttt
     2821 gatggcgtaa acatccttga gttggaagtg gatgagcgtg cgcgtatggg acttttcctt
     2881 gctatgcaat acccatcaga aatccctgga attaccaatg ctgagtttct tcgtgccgct
     2941 atgaatgcgg gtaaagaaga tgatgagaag atttcagttc gtgagtttat tactaagcta
     3001 gatgaaaaaa tggaattgct caacatgaaa gaagaaatgg cagagcgtta cctcaacgaa
     3061 ggcttctctg gtggtgagaa aaaacgcaat gaaattcttc aacttttgat gttggagcca
     3121 acatttgctc ttttggacga gattgactca ggtcttgata ttgacgctct taaagttgtg
     3181 tctaaaggtg tcaatgccat gcgtggtgaa ggttttggtg ctatgatcat cactcactac
     3241 caacgtcttt tgaactatat cacacctgat gtggtacacg tgatgatgga aggtcgtgtt
     3301 gtcctttctg gtggtccaga attggctgcg cgtttggaac gtgaaggata cgcaaaatta
     3361 gctgaagaac ttggctacga ctacaaggaa gaattgtaat tccctcgtat cttttaggag
     3421 aagtaaatga ctagagaaaa tattaaactt ttttcagaaa tgcatgctga accaagctgg
     3481 ttggctgatc tccgtcaaaa agcttttgac aagattgaga ctttggaatt accagttatt
     3541 gagcgtgtca aattccaccg ttggaatctg ggtgatggaa cgattacaga aaatgagcca
     3601 tcagcaaatg ttccagattt cacagcttta gatcatcact tgaagttggt gcaagtagga
     3661 actcaaactg ttttcgaaca aactccagtt gagttagctg aacagggtgt tgtcttcaca
     3721 gactttcact cagctttaga agaaattcca gagctgatcg aagaattctt catgtcatct
     3781 gttaagtatg atgatgacaa gttggcggct taccacacag cttactttaa cagtggtgct
     3841 gtactctata ttccagataa cgtagaaatc acagagccaa ttgaaggaat tttctaccaa
     3901 gatagcgata gcaatgtgcc gtttaacaag catattatga ttatcgctgg taaaaattct
     3961 aagattaatt atctggagcg tttagagtca cgcggtgaag gaagtgccaa agcaactgct
     4021 aatatcacag tagaagtgat tgcacgttca ggtgcgcaag tcaagtttgc tgctatcgac
     4081 cgactaggtg aaaacgtcac tgcctacatt agccgtcgtg gtaaattagg caacgatgca
     4141 agtattgact gggctatcgg tgtcatgaac gaaggaaatg tcgttgctga ttttgatagt
     4201 gacttgattg gtaatggtag ccatgctgac ctgaaggttg tagctctttc aagtggtcgt
     4261 caggtacaag ggattgatac tcgtgtaact aactatggct gcaactctat cggaaatatc
     4321 ctacaacatg gggttatcct tgaaaaagca actttgactt tcaatggtat cggccacatc
     4381 atcaagggtg ctaagggagc agatgcccaa caagagagcc gtgttctcat gctttcagac
     4441 caagcgcgtt cagatgctaa cccaattctt ttgattgatg aaaatgatgt cactgcagga
     4501 cacgcagcgt ctatcggaca agttgatcca gaagacatgt attacctcat gagtcgtgga
     4561 ttggataagg caactgcaga gcgtttggtt gttcgtggtt tccttggatc tgttatcgtg
     4621 gagattccag tcaaggaagt tcgtgatgaa atgattgcaa ctatcgaaga gaaattgtca
     4681 aaacgctaag gggaagccta tgttagatgt agaagcgatt cgcaaggatt ttccaatttt
     4741 agatcagatt gtcaatgatg aacctctggt ctatctggac aatgctgcga cgacacaaaa
     4801 accactagta gttctgaaag ttattaacag ctactatgag caggacaatg ccaatgttca
     4861 ccgtggtgtc cataccttag cggaacgagc gacagcttct tatgaagctg ctcgtgaaac
     4921 cattcgtaag tttattaatg caggctctac aaaggaagtt ctttttacca gaggaacgac
     4981 aaccagcctt aactgggtag cacgctttgc tgaggaaatt ctcactgagg gagaccaggt
     5041 cttgatttca gtaatggaac accattctaa tatcattcca tggcaggaag cttgtcgaaa
     5101 gactggagca gagcttgtct atgtctatct taaagacggt gccttggata tggaggattt
     5161 gcgagctaaa ttgactgata aggttaaatt tgtttcccta gctcatgcct ccaatgttct
     5221 tggtgtggtc aatccgatca aggaaatcac tcaattagcc caccaagttg gggcaattat
     5281 ggtagtggat ggtgctcaat ctacacctca tatgaagatt gatgtccagg acttggatct
     5341 ggactttttc gccttttcgg gtcacaagat ggctggtccg actggtatcg gtgtccttta
     5401 cggcaaagaa aagtatcttg agcaaatgtc tccagtagaa tttggcggcg agatgattga
     5461 ttttgtctac gagcaatttg ctagttggaa ggaattgcct tggaaatttg aggctggaac
     5521 gccaaatatg gcaggagcta ttggacttgc gactgcagtt gattatctgg aaaagattgg
     5581 tatggatgcc gttgaagctc atgaacagga attgattgcg tacgtctatc caaaactgca
     5641 ggcaattgag ggattgacca tttacggttc tcaggatttg gctcaacgtt cgggtgttat
     5701 tgcctttaac ctaggtgatc tccatcctca cgatcttgcg acggctctgg attatgaagg
     5761 agtggctgtt cgtgctggtc accattgtgc gcaacccttg cttcagtatt tggaagtccc
     5821 agcaacagct cgtgcaagtt tttatatcta caataccaag gcagattgcg acaaactagt
     5881 cgatgcccta caaaagacaa aggagttttt caatggcact ttctaaacta gatagccttt
     5941 atatggcagt ggtagcagac cattcgaaaa atccacatca ccaagggaag ttagaagatg
     6001 ctgagcaaat cagtctcaac aatccgactt gtggggatgt catcaacctc tctgtcaagt
     6061 ttgatgcaga ggaccgtttg gaagatattg cttttctaaa ttcaggatgc acgatttcaa
     6121 ctgcttctgc tagtatgatg acagatgccg ttttaggaaa aaccaaacaa gaaattttag
     6181 aactggcgac tattttttct gaaatggttc aagggcaaaa agatgagcgt caaggccaac
     6241 ttggagacgc ggcattcttg tcaggtgttg ccaaattccc tcaaagaatc aagtgtgcaa
     6301 ccctagcttg gaatgccctt aagaaaacaa ttgaaaatca agaaaaacag taagacaagt
     6361 ttcttttgtc ttatgaatta ttagaaatga agaaagaaag gatactatgg ctgaagaaag
     6421 agtagaacca aaaccaattg accttggtga atataaattt ggtttccatg acgatgtaga
     6481 gcctgtctta tcgacaggaa aaggactcaa cgaaggtgtt attcgtgaat tatctgctgc
     6541 taagggtgag cctgagtgga tgttggagtt ccgtttgaag tcttatgaaa ccttcaaaaa
     6601 aatgcccatg caaacttggg gagcagactt gtcagagatt gactttgatg acttaatcta
     6661 ctaccaaaaa ccatctgaca aaccagcccg ttcttgggat gatgtacctg aaaagattaa
     6721 agaaaccttt gaacgtatcg ggattccaga agctgaacgt gcttatttag caggggcttc
     6781 tgcccagtac gagtcagaag tggtttacca caacatgaag gaagagttcc aaaaattagg
     6841 tattatcttt acagatacag attccgcact caaggaatac ccagacttat ttaaacaata
     6901 ctttgcgaag ttggtaccgc cgacagataa caagttggca gccctcaact cagcagtatg
     6961 gtcgggtgga acttttatct acgtgccaaa aggtgtcaag gtagatattc cacttcaaac
     7021 ttatttccgt atcaataacg aaaatatagg tcagttcgaa cgtaccttga ttatcgttga
     7081 tgagggagca agcgtccact acgtagaagg atgtacagca ccaacatatt caagcaatag
     7141 cttacacgct gccattgtag aaatttttgc tttggacgga gcttatatgc gttatacaac
     7201 tatccaaaac tggtctgata acgtctataa cttggtaaca aagcgtgcta aggctcaaaa
     7261 ggatgccact gttgagtgga ttgatggaaa cttgggtgcc aaaacgacta tgaaatatcc
     7321 atctgtttac cttgatggag aaggagcgcg tggtaccatg ctctctatcg cctttgctaa
     7381 tgcagggcaa caccaagaca cgggtgctaa gatgattcac aatgctccac ataccagctc
     7441 gtctattgtg tctaaatcca tcgctaaagg tggaggaaag gttgactacc gtggacaagt
     7501 cacctttaac aagaactcta agaaatctgt ttcccacatt gaatgtgata ccattatcat
     7561 ggatgacttg tcagcatcag atactattcc atttaatgaa attcacaact cgcaagtagc
     7621 tttggaacac gaagccaaag tatctaagat ttcagaagag caattgtatt atctcatgag
     7681 ccgtggattg tcagaatctg aggcaactga aatgattgtc atgggatttg tagaaccctt
     7741 tacaaaagaa cttccaatgg aatacgcagt tgagctgaac cgcttgatta gctatgaaat
     7801 ggagggatca gttggataaa atttgatttt atactcttcg aaaatctctt caaaccacgt
     7861 cagcatcgcc ttaccgtatg tatggttact gacttcgtca gtttcatcta caacctcaaa
     7921 acagtgtttt gagcaacctg cggctagctt cctagttagt tctttgattt tgagtattag
     7981 atttactcaa aatcaaggat tttgaagatg aacttgtatc aaaaaatcgc ggtttaaaat
     8041 cgcgattttt tataatttct cgttaacaaa gcggacaaac tgattccacc aaacttttaa
     8101 gaagaaggct ttttcaattt tcttgtctgc taccatttcg aaactagggc gctctgtggt
     8161 gatgtaacct tgaccaatca agtccttgtc ttcataagtc aaatggccaa ccactgttcc
     8221 agcttcaagt ggtgctggga ttgctttgga atcaggtgtg aattgaacag attgggaaga
     8281 ttgattccca acacgttcga ttagatagat atcctctgga gccactgcag ttactgtatc
     8341 ttcttttcca tcttgtacag gggctttgct atcttgatag gcatcgcctt gttgaacgat
     8401 tttgcgaagt gtaaatgtag aagaaatata atccattagg gaagatgtag ctgtaaatcg
     8461 agcgtaagga ttattgtctt gatgatctgc atttaaaaca actgtgatga ctctcatgcc
     8521 tttttcaaca gtagtaccaa caaaagactc tccagcctta tctgttgttc ctgtttttag
     8581 cccatcaaaa ccaccacggt aagcaggcat accttctaac atgtagttgg ttgaagtgat
     8641 tgtcattcca gcaaaagtag aagaaggttt ttttgtgatt tccaagactt gtgggtattt
     8701 tttgatgagg ttgcgagcaa caatagcgac atcataagca ctaagcttat tttcctcatc
     8761 ttttttagaa cctgggtaaa tgttatcccc tagagtttca ttgttaagac ctgtcgtatt
     8821 gacaacagtg gcatcctgaa ttccccattc caagagtttt gcccgcatca tatcgacgaa
     8881 atctttttct gagccagcaa ttttctcagc tagggcaata gcggcgctgt tggcactaga
     8941 taccagagtt gcttcaagca actcttcgac agtataatta cgggcctcca taggaatatt
     9001 actggcttca gaatttgtcg tcaattgata aggataatca gaaatatcta caggagtgga
     9061 gagggtaata cttccgtttt ccaaagcttc atagaccaga taaacagtaa tcaattttgt
     9121 tatggaagca atttcgacag gttgcgttgc atccttctca tagagaattt taccagtatt
     9181 tgcctcaaca gcaatcgcat gtttagcggc aatggtaaaa tcttgagcaa cagcagtaga
     9241 agcaccccct aaaagagaga cagttaacaa agttaaaaat atttttttca tagtagtctt
     9301 attctatcat aaagaaaaaa aatattcttg ctttaataat tcatctgtta agctttttga
     9361 aaatatggta aaataaagtg agggaggtaa ctcatgtttc gtagaaataa attatttttt
     9421 tggaccacag aaattttact cttaaccatc atcttttatc tatggagaca gatggggtct
     9481 ttgattaacc cttttgttag cgtgcttaat acaattatga ttccattttt attagggggc
     9541 tttctttatt atttgacaaa ccctattgtt actttcttaa ataaagtctg taaactcaat
     9601 cgtttgcttg gtattttaat taccttgtgt actttggtct ggggaatggt cataggtgtt
     9661 gtctatctct tacctatttt gattaatcag ttatctagtt tgattatatc tagtcaaact
     9721 atttatagtc gagtacaaga cttaatcata gacttatcta attatcctgc gctccagaat
     9781 ttggatgtag aagctacaat tcagcagtta aacttatcct atgttgatat tcttcaaaat
     9841 atcctaaata gcgtatcaaa tagtgtgggg agcgtcttgt cagctcttat cagtactgtt
     9901 ttgattttga ttatgactcc agtttttttg gtttatttct tattagatgg acataaattc
     9961 ttgcccatgc ttgaaagaac gattctaaag agggatcgct tgcatattgc aggcttatta
    10021 aagaatttaa atgcgacgat tgctcgctat attagtggag tttcgattga cgcaatcatt
    10081 ataggttgtt tagcttatat tggctatagt attattggtt taaaatatgc tttagttttt
    10141 gccatttttt ctggtgtagc caatttaatt ccttatgtgg ggccaagtat tggtttgatt
    10201 cctatgatca tcgcaaatat attcactgat ccccatagac tgctgattgc agtgatttat
    10261 atgcttgttg ttcagcaggt agatggcaat atcttatatc ctcgaatcgt aggaagtgtt
    10321 atgaaggttc atccaatcac gattttagtt ttacttttgt tgtcaagcaa tatctatggt
    10381 gtagttggaa tgattgtcgc agtgccaacc tattctatct tgaaagaaat ttctaagttc
    10441 ttatcccgtt tgtatgaaaa tcataaaata atgaaagaac gagaaagaga attagctaag
    10501 taaaagtcag gagaaccctg atttttcttt actggaagtg gcctttagat tagaagactg
    10561 aaaataagtt aaagtcttaa actaattttc acagctaaga atagtagaag ttaatctgat
    10621 aaaaatcgaa aaaaccagtg gaattccgtg tcagggtaag ttccactggt tttcatagtc
    10681 tattaaagtt cgaatgaaac ct
//
LOCUS       DAVLVP010000059        10677 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.059, whole genome shotgun sequence.
ACCESSION   DAVLVP010000059 DAVLVP010000000
VERSION     DAVLVP010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10677
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.059"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            1..620
                     /locus_tag="VYN69_001449"
                     /pseudo
     CDS             1..620
                     /locus_tag="VYN69_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            520..966
                     /locus_tag="VYN69_001450"
     CDS             520..966
                     /locus_tag="VYN69_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1533704.1"
                     /translation="MRKHGLISKSTSKRYYQVAIPFTRLFCPALVSIDYINCLSETTD
                     KNWHKSDRIFVTNIGKPVHSSILSKSLQRANERLKKPIPKHLPPHIFRHTTISILSEN
                     KIPLKTITDRVGHSDSEVTTSIYTQVTKNMKDEAINVLDKVMKKIF"
     gene            complement(1104..2408)
                     /gene="eno"
                     /locus_tag="VYN69_001451"
     CDS             complement(1104..2408)
                     /gene="eno"
                     /locus_tag="VYN69_001451"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002985288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEV1533705.1"
                     /translation="MSIITDVYAREVLDSRGNPTLEVEVYTESGAFGRGMVPSGASTG
                     EHEAVELRDGDKSRYGGLGTQKAVDNVNNIIAEAIIGYDVRDQQAIDRAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEIPLYSYLGGFNTKVLPTPMMNIINGGSHSDAPI
                     AFQEFMILPVGAPTFKEALRYGAEIFHALKKILKSRGLETAVGDEGGFAPRFEGTEDG
                     VETILAAIEAAGYVPGKDVFIGFDCASSEFYDKERKVYDYTKFEGEGAAVRTSAEQID
                     YLEELVNKYPIITIEDGMDENDWDGWKALTERLGKKVQLVGDDFFVTNTDYLARGIQE
                     GAANSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDQLGEVAEYRGLKSFYNLKK"
     gene            2572..3018
                     /locus_tag="VYN69_001452"
     CDS             2572..3018
                     /locus_tag="VYN69_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000131081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEV1533706.1"
                     /translation="MTDLSKQLLEKAHGGLKINPDEQRRYLGTFEERVLGYVDIDTAN
                     SPQLEKGFLFILENLQEKAEPLFVKISPTIEFDKQVFYLKEAKETDSQATIVSEEHIT
                     SPFGLVIHSNAPVQVEEKDLRLAFPKLWEVKKEEPAKTSLWKKWFS"
     gene            complement(3015..4130)
                     /locus_tag="VYN69_001453"
     CDS             complement(3015..4130)
                     /locus_tag="VYN69_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000706318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEV1533707.1"
                     /translation="MKIVIAPDSFKESLTAQQVAEAIKRGFQQSIADVECLLCPVGDG
                     GEGTVDAIRHSLDLKEKWIQVTDPFGQKEAMRYFQKGELALFEVADLVGLGKIPLEKR
                     NPLQIQTCGIGELILHLISKGIKDIYIGVGGTASNDGGLGIAAGLGYQFYDRDGNVLP
                     ASGQSLLNLASVSTENRYKIPEGVQIHILADVVSPLCGYQGATYTFGNQKGLHPTMFA
                     VVDQAIQDFYEKFSPATLEIKGAGAGGGLAGGLCAFAQASIVSGIDTCLDLINFDKKV
                     SDADLVVVGEGRLDSQSFAGKAPIGVAKRTPVGVPVIAICGSLAEDLPPLPFENIQAV
                     FSILEKSEPLEDSLKNASLYLERTAANIGHLLNMCKI"
     gene            complement(4139..4468)
                     /locus_tag="VYN69_001454"
     CDS             complement(4139..4468)
                     /locus_tag="VYN69_001454"
                     /inference="COORDINATES: protein motif:HMM:NF012905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase family protein"
                     /protein_id="HEV1533708.1"
                     /translation="MERLAKSLGIAYFTANQLEVKEGLLTGKLVGQIISPQVKKETLE
                     KWRKKLKLSKERTVAIGDGVNNLLMLKSAELGIAFCAKEVLKKEIPHHVDKRDFLEVL
                     PLIDCLE"
     gene            complement(4413..4784)
                     /locus_tag="VYN69_001455"
     CDS             complement(4413..4784)
                     /locus_tag="VYN69_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IB family phosphatase"
                     /protein_id="HEV1533709.1"
                     /translation="MSQVKGLCVLDVDGTLILEEVIDLLGREAGHEAEISQITSRAMR
                     GELVFESSLRKRVSLLEGLPILVFDNVFNSIHLSLNVPEFISILQKNGILVGLVSGGF
                     TPIVGEISKIPWYCLFHCQPA"
     gene            complement(4843..6276)
                     /gene="glgA"
                     /locus_tag="VYN69_001456"
     CDS             complement(4843..6276)
                     /gene="glgA"
                     /locus_tag="VYN69_001456"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HEV1533710.1"
                     /translation="MKILFVAAEGAPFSKTGGLGDVIGALPKSLVKAGHEVAVILPYY
                     DMVEAKFGNQIEDVLHFEVSVGWRRQYCGIKKTVLNGVTFYFIDNQYYFFRGHVYGDF
                     DDGERFAFFQLAAIEAMERIDFIPDLLHVHDYHTAMIPFLLKEKYRWIQAYEDIETVL
                     TIHNLEFQGQFSEGMLGDLFGVGLERYADGTLRWNNCLNWMKAGILYANRVSTVSPSY
                     AHEIMTSQFGCNLDQILKMESGKVSGIVNGIDADLYNPQTDALLDYHFNQEDLSGKAK
                     NKAKLQERVGLPVRADVPLVGIVSRLTRQKGFDVVVESLHHILQEDVQIVLLGTGDPA
                     FEGAFSWFAQIYPDKLSTNITFDVKLAQEIYAACDLFLMPSRFEPCGLSQMMAMRYGT
                     LPLVHEVGGLRDTVRAFNPIEGSGTGFSFDNLSPYWLNWTFQTALDLYRNHPDIWRNL
                     QKQAMESDFSWDTACKSYLDLYHSLVN"
     gene            complement(6273..7412)
                     /gene="glgD"
                     /locus_tag="VYN69_001457"
     CDS             complement(6273..7412)
                     /gene="glgD"
                     /locus_tag="VYN69_001457"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004260264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase subunit
                     GlgD"
                     /protein_id="HEV1533711.1"
                     /translation="MKIDKYSAILGNTVGFHNMSTLTDHRPVASLPFGGKYRLIDFPL
                     SSLANAGVRSVFGIFQQDNISSVFDHIRSGREWGLSTLLSHYYLGIYNTRVESSTVGK
                     EYYQQLLTYLKRSGSNQTVALNCDVLINIDLNQVFHLHSTTKEPITVVYKKLAKKDIS
                     EVNAILDVDETDHVLSHKLFDSKSTAEMFNMSTDIFVVDTPWLIEHLEEEAKKEHPKK
                     LRYVLRDLAVKEGAFAYEYTGYLANIHSVKSYYQANIDMLESQKFYSLFSPNQKIYTK
                     VKNEEPTYYANTSKVSTSQFASGSIIEGQVANSVLSRNIHVHKDSLVKDSLLFPRVVI
                     GKGAQVEYAILDKGVEVEPGVVIRGTAEHPVVVKKGAKVTEDIHS"
     gene            complement(7402..8544)
                     /locus_tag="VYN69_001458"
     CDS             complement(7402..8544)
                     /locus_tag="VYN69_001458"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000787267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HEV1533712.1"
                     /translation="MKNKMLALILAGGQGTRLGKLTQSIAKPAVQFGGRYRIIDFALS
                     NCANSGIHNVGVVTQYQPLALNNHIGNGSSWGLDGINSGVSILQPYSASEGNRWFEGT
                     SHAIYQNIDYIDSVNPEYVLILSGDHIYKMDYDDMLQSHKDNNASLTVAVLDVPLKEA
                     SRFGIMNTDANNRIVEFEEKPAQPKSTKASMGIYIFDWQRLRNMLVAAEKSKVGMSDF
                     GKNVIPNYLESGESVYAYEFSGYWKDVGTIESLWEANMEYISPENALDSRNRQWKIYS
                     RNLISPPNFLGANAHVEDSLVVDGCFVDGAVKHSILSTGAQVREGAEVLDSVIMSGAI
                     IGQGAKIKRAIIGEGAIISDGVEIDGTDEVQVVGYNEVVGVATDED"
     gene            complement(8534..10462)
                     /gene="glgB"
                     /locus_tag="VYN69_001459"
     CDS             complement(8534..10462)
                     /gene="glgB"
                     /locus_tag="VYN69_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching protein GlgB"
                     /protein_id="HEV1533713.1"
                     /translation="MDNREALKTFMTGENFYLQHYLGAHREELNGEHGYTFRVWAPNA
                     QAVHLVGDFTNWIENQIPMVRNDFGVWEVFTNMAQEGHIYKYHVTRQNGHQLMKIDPF
                     AVRYEARPGTGAIVTELPDKKWRDGLWLARRKRWGFVERPVNIYEVHAGSWKRNPDGS
                     PYSFAQLKDELIPYLVEMNYTHIEFMPLMSHPLGLSWGYQLMGYFALEHAYGRPEEFQ
                     DFVEECHTHNIGVIVDWVPGHFTINDDALAYYDGTPTFEYQDHNKAHNHGWGALNFDL
                     GKNEVQSFLISCIKHWIDVYHLDGIRVDAVSNMLYLDYDNAPWTPNKDGGNLNYEGYY
                     FLQRLNEVIKLEYPDVMMIAEESSSATKITGMKEIGGLGFDYKWNMGWMNDILRFYEE
                     DPIYRKYDFNLVTFSFMYVFKENYLLPFSHDEVVHGKKSMMHKMWGDRYNQFAGLRNL
                     YTYQICHPGKKLLFMGSEYGQFLEWKSEEQLEWSNLEDPMNAKMKYFTSQLNQFYKDH
                     RCLWEIDTSYDGIEIIDADNRDQSVLSFIRKGKKGEMLVCIFNMVPVERKDFTIGLPV
                     AGIYEEVWNTELEEWGGVWKEHNQTVQTQEGLWKDYEQTLTFTLPAMGASVWKIKRRL
                     KSTKTVTNKNQKGVENEK"
BASE COUNT         3252 a         2342 c         1893 g         3190 t
ORIGIN      
        1 actgaaatag cttctgaatt tggctgtcat ccaactacca tccactatgc gctcaaagct
       61 atgggctaca ctcgaaaaaa agaaccacac ctactatgaa caagagccag aaaaagtagc
      121 cttatttctt aagaatttta atagtttaaa gcacctagca cctgtttaga ttgatgaaac
      181 aggattcgat acttattttt atcgagaata tggtcgctca ttaaaaggtc agttaataag
      241 aggtaaagta tctggaagaa gatatcagag gatttctttg gttgcaggtc taacaaatga
      301 atggtgagtt aatcgctcca atgacttacg aagagacgat gacgagcgac ttttttgaag
      361 cttggtttca gaagtttctc ttaccaacat taaccacacc atcggttatt attatggata
      421 atgcaagatt ccatagaatg ggtaagttag aacttttatg cgaggagttt ggtcataaac
      481 ttttacctct tcctccctac tcgcctgagt acaatcctat tgagaaaaca tgggctcata
      541 tcaaaaagca cctcaaaaag gtattaccaa gttgcaatac cttttacgag gctcttttgt
      601 cctgctcttg tttcaattga ctatataaat tgtctaagcg aaacaaccga taagaattgg
      661 cacaaaagcg accgtatttt tgttaccaat ataggaaaac cagttcatag ttctatcttg
      721 agcaagtctc tccagcgagc aaacgaacgc cttaaaaaac caattcccaa acatctgccc
      781 cctcacatct tcagacacac cactattagc atcttatcag aaaataaaat tcctttaaaa
      841 acaatcacgg acagggttgg tcattccgac tctgaagtca ctacttccat ctacacccag
      901 gtcacaaaga atatgaaaga tgaagcaatc aatgtactgg ataaagttat gaaaaagatt
      961 ttttaaaaag ttttgtccct tttttgtcct ctaaatacaa aaatagccct tcggataaaa
     1021 tccgaggggc tagaaacgtt gttaaatcaa cggccgaact tttgaatttc atggttcggg
     1081 ataaaatagt tcactgaact attttatttt ttaaggttgt agaatgattt caatccacga
     1141 tattcagcta cttcaccaag ttggtcttcg atacgaagca attggttgta tttagcgatg
     1201 cggtctgtac gtgaaagtga accagtcttg atttgtcctg cgttagttgc aactgcaata
     1261 tcagcgattg ttgaatcttc agtttcacct gaacggtgtg atacaacagc agtgtaacca
     1321 gcttctttag ccatttcgat agcttcaaaa gtttcagtaa gagtaccgat ttggttaact
     1381 ttgataagga ttgagttagc agcaccttct tggataccac gtgcaaggta gtcagtgttt
     1441 gttacgaaga agtcgtcacc aacaagttgt actttcttac caagacgttc agtaagagct
     1501 ttccaaccat cccagtcgtt ttcatccata ccatcttcaa tagtgatgat tgggtatttg
     1561 ttaaccaatt cttcaaggta gtcgatttgt tctgcagatg tacgaacagc agcaccttca
     1621 ccttcaaatt tagtgtagtc gtaaacttta cgttctttat cgtagaattc tgatgaagca
     1681 cagtcaaatc cgataaatac gtctttacct ggtacatatc cagcagcttc aatcgcagca
     1741 aggatagttt caacaccatc ttcagttcct tcgaaacgag gagcgaatcc accttcgtca
     1801 cctacggcag tttccaaacc acgtgattta aggattttct taagagcgtg gaagatttca
     1861 gcaccgtaac gaagggcttc tttaaatgtt ggcgcaccaa ctggcaagat catgaactct
     1921 tggaaagcga ttggagcgtc agagtgagaa ccaccgttga tgatgttcat cattggagtt
     1981 ggaagaactt tagtgttgaa tccaccaaga tagctgtaaa gtgggatttc aaggtagtca
     2041 gcagcagcac gagctacagc gatagacaca ccgaggattg cattcgcacc caatttacct
     2101 ttgttaggag taccgtcaag tgcgatcata gcacggtcaa tagcttgttg atcacgtaca
     2161 tcgtagccaa tgatagcttc agcaatgatg ttgtttacgt tgtcaacagc tttttgtgta
     2221 ccaagaccac cgtaacgaga tttgtcaccg tcgcgaagtt caactgcttc gtgttcacca
     2281 gtagaagctc ctgatggaac cataccacgt ccgaaagcac ctgattcagt gtaaacttct
     2341 acttcaagtg ttgggttacc gcgtgagtct aggacttcgc gagcgtaaac atcagtaata
     2401 attgacattt tttactctcc ttatgagtta aattttttac acctctataa taccttaaaa
     2461 cccctccttt ttcaagaaaa aacgttatct ttgtgcaact tttccttaac tttataaagt
     2521 aatcgctttc ttttgtctgt tttattctaa cttttatgat atactgtttt catgacagat
     2581 ttatcaaaac aattacttga aaaagctcat ggtgggttaa aaataaatcc ggatgagcaa
     2641 agacgctatc ttggtacttt tgaggaaaga gttcttggat atgtagatat tgacacagca
     2701 aatagccctc agttagaaaa aggcttttta tttattttag aaaaccttca ggaaaaagca
     2761 gagccactat ttgtgaagat ttcaccaact atcgaatttg ataagcaagt tttctactta
     2821 aaagaagcaa aagaaactga tagtcaagcc accatagtat ctgaagagca tattacttct
     2881 ccttttggcc tggttattca tagcaatgca ccagttcaag tagaagaaaa agaccttcga
     2941 cttgcttttc caaaactttg ggaagttaaa aaggaagaac cagccaaaac atccttatgg
     3001 aagaaatggt ttagctaaat cttgcacata tttaataagt gcccaatatt ggcagccgtg
     3061 cgctccagat agagactggc atttttcaaa ctatcttcta aaggttcact tttctccaaa
     3121 atagaaaaga cagcttggat attttcaaat ggtaggggag gtaaatcttc agcaagacta
     3181 ccgcaaatag caataacagg aactccaaca ggggttcttt ttgcaacacc tataggcgct
     3241 ttcccagcaa agctttgact gtcaagtctt ccttctccaa caacaaccaa gtcagcatct
     3301 gaaactttct tatcaaagtt gattaagtcc aagcaggtat caattccaga cacgatactt
     3361 gcctgagcaa aggcacacaa accaccagca aggcctccac ctgctcctgc tcctttaatt
     3421 tctaatgttg caggtgagaa tttttcataa aaatcttgga tcgcctgatc tacgactgca
     3481 aacatagtcg gatgtagacc tttttgattg ccaaaagtgt aagtcgcacc ttgataacca
     3541 cataagggac tcacgacatc tgctaaaata tgaatttgaa caccttcagg aattttatag
     3601 cgattttctg ttgaaacaga agctaagttt aataaggatt gaccggaagc aggcaagaca
     3661 tttccatccc tatcataaaa ttgataacct aaaccagcag caatccccag tcctccatca
     3721 ttactggccg tgccaccaac accgatataa atatctttaa tccctttaga aatgagatgg
     3781 agaatcaact ctccaatacc acaagtttgg atttgaagtg gatttcgttt ctctagcgga
     3841 atttttccaa gaccaaccaa gtcagctact tcaaatagtg ccagttcccc tttttgaaaa
     3901 tagcgcatgg cttctttttg tccaaaaggg tctgtcactt ggatccattt ttcttttagg
     3961 tcaagagaat gtcggatagc atctacagta ccttctcccc catcaccaac agggcagagg
     4021 agacattcta catctgctat cgattgttgg aagcctcttt ttattgcttc agctacctgt
     4081 tgagctgtca agctttcctt aaacgaatcc ggtgcaatta caatcttcat attttccctc
     4141 attctaaaca gtcaatcaaa ggaagaactt ctaaaaaatc cctcttgtca acatgatgtg
     4201 gtatttcttt tttgagcact tctttggcac aaaaggcgat tcctaactcc gccgacttca
     4261 acattaatag attattgacc ccatcaccga ttgccaccgt tctttcttta gaaagtttta
     4321 gtttctttct ccatttttcc agagtttctt ttttgacctg gggacttata atttgtccaa
     4381 ctaattttcc tgttaaaaga ccttctttga cttcaagctg gttggcagtg aaataggcaa
     4441 taccaaggga ttttgctaat ctctccaact attggtgtaa atccaccaga caccagacca
     4501 actaggatgc cattcttttg gagaatagag atgaactctg ggacatttag cgatagatga
     4561 attgagttga agacgttatc aaagaccaaa ataggaagac cttccaacaa ggacactctt
     4621 tttcttaaac tgctttcaaa gaccaactct cctcgcattg ctcgacttgt aatctgcgaa
     4681 atttccgcct catgacctgc ctctctccct aaaagatcaa tcacttcttc taggattaag
     4741 gttccatcta catccaaaac acacaagcct tttacttgag acatcagttc tcctctctaa
     4801 acagcctaaa aatcgtatga agtcatcata cgattttatc tattaattaa ctaaactatg
     4861 gtacaagtca aggtatgact tgcaggctgt atcccatgag aagtcactct ccatagcttg
     4921 tttttgtagg tttctccaaa tgtctggatg gtttctatac aagtccaatg ctgtttggaa
     4981 agtccaattt aaccaataag gagatagatt gtcaaagcta aagccagtac cgcttccttc
     5041 gattggattg aaagcgcgaa ctgtatctcg caagcctcca acttcatgga ccaatggcaa
     5101 ggttccataa cgcatagcca tcatttgaga caagccacac ggttcaaaac gacttggcat
     5161 gaggaagagg tcacaagcag cgtagatttc ctgagcaagt ttgacatcaa aagtgatatt
     5221 tgttgatagc ttgtctgggt aaatctgagc aaaccatgag aaagctcctt caaaggctgg
     5281 atcgccagtt cccaaaagaa caatctgaac atcttcttgc aagatatggt gaagactttc
     5341 gaccaccaca tcaaaacctt tttgacgtgt caaacgagaa acaattccca ccagtggaac
     5401 gtctgctcta acaggcaagc caactctttc ttgcaatttt gccttatttt tggctttccc
     5461 agacaaatct tcctgattga aatgatagtc taaaagagca tccgtctgag gattataaag
     5521 atcagcatca atcccattca cgataccaga tactttacca gactccattt taagaatctg
     5581 atccaaatta catccaaact gactagtcat aatttcatga gcatagctag gtgaaacggt
     5641 tgaaacacgg ttcgcataga gaatacctgc cttcatccag ttcagacagt tgttccatcg
     5701 aagggtgcca tcagcgtaac gttcaaggcc aactccaaac aaatcaccca acattccttc
     5761 tgaaaattgt ccttggaatt ctaaattatg aatggttaaa actgtttcaa tgtcctcata
     5821 ggcttgaatc caacggtatt tttccttcaa caagaaagga atcatagctg tatggtagtc
     5881 atgaacatgg agaagatcag gaataaagtc aatcctttcc atagcctcaa tggcagccag
     5941 ttggaaaaag gcaaagcgtt ctccgtcatc aaaatcaccg taaacatgac cacggaagaa
     6001 ataatattga ttgtcaataa agtagaaggt tacaccattt aatactgttt tcttaattcc
     6061 acaatactgt ctgcgccaac caacgctcac ctcaaaatga agcacatctt caatctgatt
     6121 tccaaattta gcctctacca tatcatagta gggtaaaatc actgcaactt cgtgcccagc
     6181 ttttaccagt gattttggaa gagcgccaat gacgtctccc aaaccacctg tttttgaaaa
     6241 aggtgcaccc tctgctgcta caaataaaat tttcatgaat gaatatcctc tgttacttta
     6301 gcacctttct taaccacaac tggatgttct gcagttcctc gaatcacaac accaggctca
     6361 acttcaaccc ctttgtccaa gatagcatat tcgacctgag ccccttttcc aataacaaca
     6421 cgagggaaga gcaggctatc tttaaccaag ctatccttat ggacatgaat attacgtgat
     6481 agaacagaat tagctacttg accttcaata atactaccag aggcaaactg agaagtgctt
     6541 accttagatg tattagcata gtaagttggc tcttcgtttt tgacctttgt ataaatcttt
     6601 tggtttggtg agaaaagaga atagaatttt tgtgattcaa gcatatcgat attcgcttga
     6661 taataagatt taacagagtg aatattggct agatagcccg tgtactcgta ggcgaaagct
     6721 ccctctttta cagccaaatc ccgtaaaaca tagcgcaatt tctttggatg ttctttttta
     6781 gcttcttctt ccaagtgttc aatcaaccaa ggtgtatcaa cgacaaagat atctgtagac
     6841 atattgaaca tttcagctgt tgacttgcta tcaaagagtt tatgagaaag aacatggtct
     6901 gtttcatcta catccaagat tgcatttact tctgaaatat ctttcttagc tagtttttta
     6961 taaactacag tgataggctc ttttgttgta ctatgtaggt ggaaaacttg gttcaaatca
     7021 atgttaataa gaacatcgca gttgagggca accgtttggt ttgagccaga acgtttcaaa
     7081 taagtaagaa gctgttggta gtattctttt ccaactgtac tactttctac acgggtattg
     7141 taaattccta gatagtaatg actaagaagg gttgataagc cccactcgcg tcctgaacga
     7201 atatggtcaa atactgagct gatattatcc tgctggaaaa taccaaagac actacgaaca
     7261 cctgcattag caaggcttga aagtgggaag tcaatcaaac gatatttccc accaaatggc
     7321 aaacttgcta ctggacggtg gtccgtcaat gtcgacatat tgtgaaaacc aactgtattt
     7381 cctaaaatgg cagaatattt atcaatcttc atctgttgct acccccacta cttcattata
     7441 tcctacaact tgtacttcat ctgttccatc aatttcgaca ccgtcagaaa taatcgcacc
     7501 ttcaccaata atggcacgtt taatcttagc tccttgacca atgatagctc cactcatgat
     7561 aactgaatca aggacttccg ctccttcgcg aacttgcgcg cctgttgaaa ggatagaatg
     7621 tttaacagct ccatcaacga aacatccgtc tacaactaat gagtcttcca catgagcatt
     7681 tgccccgagg aagtttggtg gtgaaatcaa gtttcttgag taaatcttcc attgacggtt
     7741 acgactatcc aaggcatttt ctggagaaat atactccatg ttcgcttccc aaagtgactc
     7801 aatagtacca acatctttcc aataaccact aaattcgtaa gcataaacac tttcacctga
     7861 ctcaaggtaa tttggaatga catttttacc aaagtctgac atgccaacct tgctcttttc
     7921 agcagcgact aacatattac gaaggcgttg ccaatcaaaa atgtagattc ccatagaagc
     7981 ttttgtagat ttaggttgag ctggtttttc ttcaaattca acaatacgat tgttagcatc
     8041 tgtgttcatg ataccaaaac ggcttgcttc tttaagaggg acgtctaaaa ctgctactgt
     8101 caagctggca ttattatcct tatgagactg gagcatatca tcatagtcca ttttgtagat
     8161 gtggtcccca gacaaaatca agacatactc aggattgaca ctgtcgatat agtcgatatt
     8221 ttggtaaata gcgtgactag ttccctcaaa ccaacgattt ccttcacttg cagaataagg
     8281 ttgaagaata gagacgcctg aattaatacc gtctagtccc cagcttgaac cattcccaat
     8341 atggttgttg agagcaagtg gttgatactg tgtaacgacc ccaacattat gaatccctga
     8401 gttggcacag tttgataggg caaagtcaat gatacggtag cgcccaccaa attgcacagc
     8461 tggttttgcg atgctttgag tgagtttacc gagacgagtt ccttgcccac cagcaagaat
     8521 caaagctaac attttatttt tcattttcta ctcctttttg gtttttattt gtgacggttt
     8581 tagtagattt caagcgacgt ttgattttcc atacacttgc tcccatagcc ggtagagtaa
     8641 aggttaaggt ctgctcataa tctttccata ggccttcttg cgtttgaaca gtttgattat
     8701 gttctttcca aacgcctccc cactcttcca actcagtatt ccatacttct tcgtaaattc
     8761 ctgcaacggg tagtccgatt gtaaaatctt tccgctcaac aggtaccatg ttaaagatac
     8821 agactaacat ttctcccttt ttacccttac gaataaagga aagaacactc tggtctcgat
     8881 tatccgcatc aatgatttca ataccatcat agctggtatc gatttcccac agacagcgat
     8941 gatctttgta aaactggttt agctgagaag tgaaatactt catcttagca ttcattgggt
     9001 cttctaggtt agaccattcc aactgttctt cagatttcca ttctaggaat tgaccgtatt
     9061 cgctacccat gaagagcaat ttcttaccag ggtgacaaat ttggtacgta tagagattgc
     9121 gcaagcctgc gaattgattg taacgatctc cccacatctt atgcatcata ctcttcttgc
     9181 catgaaccac ttcatcgtgc gagaatggca agagataatt ctccttgaaa acatacataa
     9241 agctgaaagt caccaggtta aagtcatatt tacgataaat cggatcttct tcgtagaaac
     9301 ggaggatatc attcatccag cccatgttcc atttgtagtc aaatcctaga ccaccaatct
     9361 ctttcattcc cgtaatcttg gtcgcagacg aactttcttc tgcaatcatc atcacatctg
     9421 gatattctaa cttaataact tcattcaagc gttgaagaaa ataataacct tcatagttaa
     9481 gatttccgcc atctttatta ggtgtccatg gagcattatc atagtccaaa tagagcatgt
     9541 tgctaacagc atccacacga ataccatcca aatgatagac atcaatccaa tgcttaatgc
     9601 aagaaattaa gaaggactgg acttcatttt ttccaaggtc aaaattaagg gcaccccaac
     9661 catggttatg agccttatta tggtcttggt attcaaaagt cggtgtccca tcataatagg
     9721 ctaaggcatc atcgttgatg gtaaagtgac ctggtaccca gtccacaata accccaatat
     9781 tatgggtatg acactcctcg acaaaatctt gaaactcctc tggtctgcca taagcatgct
     9841 ctaaagcgaa gtaacccata agctgatacc cccaactcaa gcccaaagga tgggacatca
     9901 agggcataaa ctcaatatga gtatagttca tttcaacgag ataaggaatg agttcatcct
     9961 tgagctgggc aaaactataa ggactgccat caggatttct cttccaagag ccagcatgaa
    10021 cctcgtaaat attgacagga cgttcaacaa agccccaacg ttttcttcgt gccagccaaa
    10081 gtccatccct ccatttctta tcaggaagct ctgttacgat tgcccctgtt cctggacgag
    10141 cctcatacct gacagcaaaa gggtcaatct tcatcagttg atgaccattt tgacgtgtga
    10201 catgatattt gtaaatatgc ccttcttgag ccatattggt aaagacttcc cagaccccaa
    10261 agtcatttct taccattgga atctgatttt caatccagtt ggtaaaatca ccaaccaagt
    10321 gaacagcctg agcattaggt gcccaaacac ggaaggtata gccatgctct ccatttagtt
    10381 cttccctatg tgctcctaga taatgttgga gataaaaatt ttcacccgtc ataaaggttt
    10441 ttaatgcttc tctattatcc atatactccc cttctcctgt aagcgttttc tatgttttta
    10501 ttatactacc tttttagaga agattcaagt aaattactat acttctttaa ttattttgaa
    10561 aatctacaac aagttcactt actcgttcaa ttgtaaatca atattttttc aaaaaattgc
    10621 gaaaacgcct ttctttttct actatagtga aatgaaataa aacatgcgca aatcgat
//
LOCUS       DAVLVP010000060        10606 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.060, whole genome shotgun sequence.
ACCESSION   DAVLVP010000060 DAVLVP010000000
VERSION     DAVLVP010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10606
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.060"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            199..1077
                     /gene="truB"
                     /locus_tag="VYN69_001460"
     CDS             199..1077
                     /gene="truB"
                     /locus_tag="VYN69_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007516878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEV1533714.1"
                     /translation="MNGIINLKKEAGMTSHDAVFKLRKILGTKKIGHGGTLDPDVVGV
                     LPIAVGKATRMVEFMQDEGKIYEGEIILGYSTTTEDASGEVVAKTPVLSSLDEKLVDE
                     AIASLTGPITQIPPMYSAVKVNGRKLYEYARAGQEVERPERQVIIYQFERTSPISYDG
                     QLARFTFRVKCSKGTYIRTLSVDLGEKLGYAAHMSHLTRTSAAGLQLEDALALEEIAE
                     KVEAGQLDFLHPLEIGTGDLVKVFLTPEEATEVRFGRFIELDQTDKELAAFEDDKLLA
                     ILEKRGNLYKPRKVFS"
     gene            1318..1620
                     /locus_tag="VYN69_001461"
     CDS             1318..1620
                     /locus_tag="VYN69_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEV1533715.1"
                     /translation="MMRNEFRERVEQLLQQKEINENSELSHLFRLAIQNLDRNEKYQT
                     VMANLSQGLSLYLMTHHYQAPKSVIDFGLWIAKAPSQERGRLAFLQMLAQTLQGFR"
     gene            1728..2366
                     /gene="udk"
                     /locus_tag="VYN69_001462"
     CDS             1728..2366
                     /gene="udk"
                     /locus_tag="VYN69_001462"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001181378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEV1533716.1"
                     /translation="MQNRPIIIGVTGGSGGGKTSVSRAILSHFPDEKISMIEHDSYYK
                     DQSHLTFEERVKTNYDHPFAFDTDLMIEQIKELLAGRPVDIPTYDYTEHTRSSKTYRQ
                     EPQDVFIVEGILVLEDKRLRDLMDIKIFVDTDDDVRIIRRIKRDMEERGRSLDSVINQ
                     YLGVVKPMYHQFIESTKRYADIVIPEGVSNTVAIDLLTTKIAKILEEARNSK"
     gene            2494..3834
                     /locus_tag="VYN69_001463"
     CDS             2494..3834
                     /locus_tag="VYN69_001463"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEV1533717.1"
                     /translation="MEKYLSVTTLTKYLKMKFDKDPYLERVYLTGQVSNFRKRPTHQY
                     FSLKDDHAVIQATIWSGIYQKLGFDLEEGMKINVIGRVQVYEPSGSYSIIIEKVEPDG
                     VGALAIQFEQLKKKLTEEGLFQERFKQALPQFSKRIGVVTSRSGAVIRDIITTVSRRF
                     PGVDILLYPTKVQGEGAAEEIARNIARANQRDDLDLLIIGRGGGSIEDLWAFNEEIVV
                     RAIFESRLPVISSVGHETDVTLADFVADRRAATPTAAAELATPVTKLDVLAHLQNQEK
                     RMVTAVRNVLSKKQEALKKCSQSVIFRQPERLYDGYLQRLDQLQLRLKQSLRTRISDN
                     KQLVQARTHQLVQLSPVTKIQRYQDRLGQLDKLLGSQMALVYDAKVAEVKRLSEALLM
                     LDTSRIVARGYAIVKKEESVVDSVESLKKKDQVTLLMRDGQVELEVKDVKTKEI"
     gene            3812..4024
                     /locus_tag="VYN69_001464"
     CDS             3812..4024
                     /locus_tag="VYN69_001464"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEV1533718.1"
                     /translation="MSKQKKFEENLAELETIVQSLENGEIALEDAITAFQKGMVLSKE
                     LQATLDKAEKTLVKVMQEDGTESDFE"
     gene            4021..4896
                     /locus_tag="VYN69_001465"
     CDS             4021..4896
                     /locus_tag="VYN69_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEV1533719.1"
                     /translation="MKKQEKLALVESALEDFYGDQQFASSLRESVLYSIHAGGKRIRP
                     FLLLEVLEALQVTIKPAHAQVATALEMIHTGSLIHDDLPAMDDDDYRRGRLTNHKKFG
                     EAMAILAGDALFLDSYALIAQADLPSQIKVDLIANLSLASGSLGMVAGQVLDMEGEHQ
                     HLSLEELQTIHANKTGKLLAYPFQAAAIIAELSPEMQVKLKTVGELIGLAFQVRDDVL
                     DVTASFEEIGKTPQKDLQAEKSTYPALLGLEESIAFCNQTLDQANEKLEEIAQQLPFE
                     TESIVSVVESLRING"
     gene            4889..5704
                     /locus_tag="VYN69_001466"
     CDS             4889..5704
                     /locus_tag="VYN69_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEV1533720.1"
                     /translation="MAKERVDVLAYKQGLFETREQAKRGVMAGLVVAVLNGERFDKPG
                     EKIPDDTELKLKGEKLKYVSRGGLKLEKALQVFDLSVDGATTIDIGASTGGFTDVMLQ
                     NSAKLVFAVDVGTNQLAWKLRQDPRVVSMEQFNFRYAEKTDFEQEPSFASIDVSFISL
                     SLILPALHRVLADQGQVVALVKPQFEAGREQIGKNGIIRDAKIHQNVLESVTAMAVEA
                     GFSVLGLDFSPIQGGHGNIEFLAYLKKEKSASNQILAEIKEAVERAHSQFKNE"
     gene            5697..6128
                     /locus_tag="VYN69_001467"
     CDS             5697..6128
                     /locus_tag="VYN69_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1533721.1"
                     /translation="MNKKERLEKIRRLVTDYQIGTQEEIVEHLKEAGITATQATVSRD
                     IKELGIVKIPLRDNTYVYELPKSIVKSLQLAEDNIESAELMDKMINLQVIPGNTAFVK
                     AQLIETFADKIFSCLTDDSSILVIARSGSLAEEIFEQVKNW"
     gene            6135..7802
                     /gene="recN"
                     /locus_tag="VYN69_001468"
     CDS             6135..7802
                     /gene="recN"
                     /locus_tag="VYN69_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEV1533722.1"
                     /translation="MLLEISIKNFAIIEAISLNFEKGMTVLTGETGAGKSIIIDAMNM
                     MLGARATTDVIRHGAPKAEIEGLFSVENSRLLQEIFDEQGLELGDEIIIRREILQNGR
                     SISRVNGQMVNLSVLRAIGQHLVDIHGQHDHEELMRPQLHIQMLDEFGDAAFWDLKET
                     YQTSFDAYRKMRKQVLEVKKNQQEHKARIEMLEFQMAEIEAANLQAGEDLALNQERDK
                     LLNHKNIADTLTNAYSMLDNEDFSSLANVRSAMNDMESVEEYDPEYREISSSLSETYY
                     VLEDISKRLEAIIEDLDFDGNRLMQVENRLDLLHTITRKYGGTVDDVLLYFAKITEEY
                     NLLTGNNLSSEDMEAELKKLEVNLVNLAGQLASARHNLAQQLEAEIKQELQDLYMEKA
                     QFQVRFSKGKFSREGNEMVEFYISTNPGEDFKPLVKVASGGELSRLMLAIKSAFSRKE
                     GKTSIVFDEVDTGVSGRVAQAIAQKIHKIGQHGQVLAISHLPQVIAIADYQFFIEKIS
                     NDHSTVSTVRLLTVEERVEEVAKMLAGDDVTEAALTQARELLRNREK"
     gene            7804..8532
                     /locus_tag="VYN69_001469"
     CDS             7804..8532
                     /locus_tag="VYN69_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000133310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEV1533723.1"
                     /translation="MTDYYVIGDVHGKAGMLEDLLKTWDGQTQLLFLGDLIDRGEDSH
                     RVLEMVKDLVDNQGAICLSGNHEYMFLTWLDDPEESYDHYRRNGGDTTINSILGRPLD
                     APVDGVEDAKQVAAEAADLVEFIRQMPFVVETDKYIFVHAGIDLTLDDWHETTDYKKV
                     WLRKPFHGAENHTGKTIVFGHTPVYGLLQQERGTSELWTTEDGKIGMDGGAVYGGVLH
                     GIVFTDQGMTEHHFIENDGFVAED"
     gene            8611..10434
                     /gene="lepA"
                     /locus_tag="VYN69_001470"
     CDS             8611..10434
                     /gene="lepA"
                     /locus_tag="VYN69_001470"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001047203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEV1533724.1"
                     /translation="MNLEELKKRQEKIRNFSIIAHIDHGKSTLADRILEKTETVSSRE
                     MQAQLLDSMELERERGITIKLNAIELNYTAKDGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNDLEIMPIINKIDLPAADPERVRTEIEDVI
                     GLDASEAVLASAKAGIGIEEILEQIVEKVPAPTGDVTAPLKALIFDSVYDAYRGVILQ
                     VRVMDGVVKPGDKIQLMSNSKTFDVAEVGIFTPKAVGRDFLATGDVGYIAASIKTVQD
                     TRVGDTVTLATNPAAEPLHGYKQMNPMVFAGLYPIESNKYNDLREALEKLQLNDASLQ
                     FEPETSQALGFGFRCGFLGLLHMDVIQERLEREFNIDLIMTAPSVIYKVNLTDGESMD
                     VSNPSEFPDPTKIATIEEPYVKAQIMVPQEFVGAVMELAQRKRGDFVTMDYIDDNRVN
                     VIYQIPLAEIVFDFFDKLKSSTRGYASFDYELSEYRPSKLVKMDILLNGDKVDALSFI
                     VHKDFAYERGKLIVDKLKKIIPRQQFEVPIQAAIGHKIVARTDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKAGKKRMKSIGSVEVPQEAFLSVLSMDEE"
BASE COUNT         3130 a         1909 c         2599 g         2968 t
ORIGIN      
        1 ctttagatga cagacgaaat gtattacaac atattcaaaa acagaacaat tgtcctcctc
       61 cagagattga ggaattacaa agaattatag ttaattaaaa tttgtaaaaa agagaataaa
      121 aggtagacga cgcctcagta aaaattggcc cggaaaaaag cgaagttcga agcactgaag
      181 ggggagtaga aagcaaaaat gaacggtatt atcaacttaa aaaaagaagc aggaatgacc
      241 tcgcatgatg cggtttttaa actgcgtaag attttgggaa ccaagaaaat tggtcatggt
      301 ggaaccttgg atccggatgt ggtgggtgtt ttgccgattg cggttggcaa ggcgacacgc
      361 atggtcgagt ttatgcagga cgagggtaag atctatgagg gggaaatcat tctgggctat
      421 tccacgacga ctgaggatgc tagtggggaa gtggtcgcaa aaacccctgt tttgtcttca
      481 ttggatgaaa agcttgttga tgaagcgatt gctagcttga ctgggcctat tactcagatt
      541 ccccctatgt attcggcagt taaggttaat ggtcgcaagc tctatgagta tgcgcgtgct
      601 ggtcaggaag tggagcgtcc agaacgtcag gtgatcattt atcaatttga gcgaacaagt
      661 ccgatttctt atgatggcca acttgcccga ttcacttttc gtgtaaaatg cagtaaaggg
      721 acttacatcc gtactttgtc agttgatttg ggtgaaaagc ttggttatgc ggctcatatg
      781 tcccatttga ctcgtactag tgctgctggc ttacaattag aagacgctct tgccttggag
      841 gaaattgctg aaaaagtaga ggctgggcaa ttagattttc tccatccttt agagattggg
      901 acaggtgatc ttgtcaaagt tttcctaact ccagaagagg ctacagaagt tcgctttggt
      961 cgttttattg agctagacca aacggacaaa gaactggctg cctttgaaga tgataaattg
     1021 ttagccattc tagaaaaacg gggcaatctc tataagccaa ggaaggtttt tagctagatc
     1081 gtttaggaat aaaaatcggg tgatagataa caattgcttg ataaaacccc atactaatag
     1141 tagaatggtt ttgggaatta taatattcca attgttgcga gttgtaggta ctcaaataat
     1201 ctatatagaa atttagaggt gtgaaatgaa gcaatttaaa attctttcag ataaatattt
     1261 agagtccatt acaggttctg gtgggaactt aggcccagga tttggtgtga taattccatg
     1321 atgcgaaatg agtttcgaga aagggtggag caacttcttc aacaaaaaga aataaatgaa
     1381 aatagtgagt tgagtcacct gtttcgtctt gctatacaaa atttagacag aaatgaaaaa
     1441 taccaaacgg tcatggccaa tttgagtcaa gggttgtcac tttacctcat gacgcatcat
     1501 taccaggcac ctaagtctgt cattgatttt ggtttatgga ttgccaaagc tcctagccag
     1561 gaaagaggga gactggcttt cttgcagatg cttgctcaaa ccctacaggg ctttcgttaa
     1621 aactgtcgaa catcagaggg aagactctgg tgttttttgc tgatgagact ataaattttg
     1681 taaaaaatgt gatagaatag acgacggata aaataacgga ggatagcatg caaaatagac
     1741 caatcattat tggagtgaca ggtggttctg gtggtggtaa aaccagtgtt tcaagagcca
     1801 ttttatcgca ttttcctgat gaaaagattt ccatgattga gcatgattca tactacaagg
     1861 atcagtctca tttgaccttt gaagagcgtg tcaaaaccaa ctatgaccat ccttttgcct
     1921 ttgatacaga cttgatgatc gagcagatta aggaattgtt ggcagggcgt ccggtggaca
     1981 tcccgaccta cgactataca gagcatacac ggagtagcaa gacctatcgt caggagcctc
     2041 aagatgtctt tatcgttgag ggcattttgg tcttggagga caagcgtctg cgcgatttga
     2101 tggatatcaa gatttttgtg gatacggatg acgatgtgcg tattattcgt cgtatcaagc
     2161 gtgatatgga ggagcgtggc cgtagccttg atagcgttat taaccagtac ttaggtgtgg
     2221 tcaaaccaat gtaccaccag tttatcgagt caactaagcg ttatgctgat atcgtcattc
     2281 ctgaaggggt tagcaatacc gtggctatcg acctgttgac gaccaagatt gcaaagattt
     2341 tggaagaagc tcgaaacagc aaataatcac atgtggaggc cttgcctcct ttttctattt
     2401 ttccctcata atggtacata aatggagatt tttaggcata tttttggtat aataataccc
     2461 atgaaagagc aagtaaatga atagtaggtg gagatggaaa agtatttatc ggtaacaact
     2521 ttgaccaagt atctgaaaat gaaattcgat aaagacccat acttggaacg ggtctattta
     2581 actggtcaag tttccaactt tcgtaaacga cctactcacc aatatttctc cctaaaggat
     2641 gaccatgcag ttattcaagc gaccatctgg tctgggattt atcagaaatt agggtttgac
     2701 ctggaagaag gaatgaagat caatgtgatt gggcgtgtac aggtctatga accaagtggt
     2761 agctactcca tcatcattga aaaagttgag cctgatgggg ttggggcgct tgcgattcag
     2821 tttgaacaac ttaagaaaaa attgacagaa gaaggcctgt ttcaagaacg cttcaagcaa
     2881 gctctgcccc aattttctaa gagaattggg gtagtaacca gccgtagtgg agccgttatt
     2941 cgagatatta tcacgaccgt cagcaggcga ttcccaggtg ttgacatcct tctttatccg
     3001 accaaggttc aaggtgaagg ggctgcggag gaaattgcta gaaatattgc gcgtgctaat
     3061 caacgggacg atttggattt gctcattatt ggtcgtggag gtggttctat tgaggatctc
     3121 tgggccttta acgaagaaat tgtggtacga gctatttttg aatctcgttt gccagttatc
     3181 tctagtgtgg ggcatgagac ggatgtgacc ttggcagatt ttgtggcaga tcgacgcgct
     3241 gcaacgccaa cagcggcggc tgaactggca acacctgtga ccaagttgga tgtattagct
     3301 catttgcaaa atcaggaaaa acggatggta acggcagtcc gaaatgttct atctaagaaa
     3361 caagaggctt tgaaaaaatg cagtcagtct gttatcttta gacaacctga gcgcttgtat
     3421 gacggttatt tgcaacgctt ggaccaactg caactgcgtt tgaaacaaag tttgcgaact
     3481 cggatttctg ataacaaaca attagttcaa gcaagaactc atcaattagt acaattatca
     3541 cctgttacca aaatccaacg ctatcaagac cgtttaggac agttggacaa gctcttaggt
     3601 agccaaatgg cgttagttta tgacgccaag gttgctgagg tcaagcgact ttcggaagct
     3661 ttgctcatgt tggatactag ccgaatcgtg gcgcgtggtt atgctattgt caaaaaagaa
     3721 gagtccgttg tagattcggt tgagagtttg aagaaaaaag accaagtaac gcttttgatg
     3781 cgagatggtc aagtagaatt agaggttaaa gatgtcaaaa caaaagaaat ttgaggaaaa
     3841 tctagcagaa ctggaaacca ttgtccaaag tttggaaaat ggtgaaattg ctctggaaga
     3901 tgcgattact gcctttcaaa agggcatggt cttgtcaaaa gagctccaag ctacgctgga
     3961 caaggctgaa aagaccttgg tcaaggtcat gcaagaagac ggaacagaaa gtgattttga
     4021 atgaaaaagc aagaaaaatt agctcttgtc gagtcggctt tggaagattt ttatggagac
     4081 cagcagtttg cctctagttt acgggagtct gttctctatt ctattcatgc tggtggcaag
     4141 cgtattcggc cttttctctt gttagaagtt ctggaagcct tgcaggttac catcaaacct
     4201 gctcacgcgc aggtagctac tgccttggag atgattcata cagggagctt gattcacgat
     4261 gaccttcctg ctatggatga tgacgattat cgaagagggc ggttaaccaa tcacaagaaa
     4321 ttcggtgaag ctatggccat tttggctgga gatgccttat tcttagactc atatgccttg
     4381 attgcgcagg cagatttgcc aagtcagatt aaggtggact tgattgccaa cttatccctt
     4441 gcttcaggta gtctgggtat ggtggcaggg caagttttgg atatggaggg cgaacaccag
     4501 cacttgtctt tggaagaact tcagactatt catgccaata agactgggaa gttactagcc
     4561 tatcccttcc aagcggcagc tattatagct gaattgtcac ctgaaatgca ggtgaagctg
     4621 aaaactgtgg gtgaattgat tggacttgct tttcaagtca gagatgatgt actggatgtg
     4681 acagctagtt ttgaggaaat cggcaagaca cctcaaaagg atctgcaggc agaaaaatca
     4741 acctatcctg ccttgttggg cttggaagag tccattgcct tttgtaacca aaccctggat
     4801 caagctaatg aaaaattaga agaaattgcc cagcagcttc cctttgaaac agaatcgatt
     4861 gtaagtgtag tagaaagttt gagaatcaat ggctaaggaa agagtggatg tactagctta
     4921 taaacagggg ttgtttgaaa cgagagagca ggccaagcga ggtgtgatgg ctggtctagt
     4981 cgtagcagtc cttaatggag aacggtttga caagccagga gagaaaattc cagatgacac
     5041 cgaattaaaa ctcaaggggg agaaactcaa gtatgtcagc cgtggtggtt tgaaactgga
     5101 aaaggccttg caggtctttg atttgtcggt ggatggcgcg actacgattg atatcggggc
     5161 ctctactgga ggttttaccg atgtcatgct acagaatagt gccaagttgg tctttgcagt
     5221 cgatgttggt accaatcagt tggcttggaa attacgccaa gacccacgag ttgtcagcat
     5281 ggagcagttc aattttcgct atgctgaaaa gactgatttc gagcaggagc cgagctttgc
     5341 cagtattgat gtgagtttca tttcccttag tctgattttg ccagccttgc accgtgtctt
     5401 ggctgatcaa ggtcaggtgg tagcacttgt caaacctcag tttgaggcag gacgtgagca
     5461 gattgggaaa aatggaatta ttcgagatgc taagattcat cagaatgtcc ttgaatctgt
     5521 aacagctatg gcagtagagg caggtttttc agtccttggc ttggactttt ctcccatcca
     5581 aggtggacat ggaaatattg aatttttagc gtatttgaaa aaagaaaagt cagcaagcaa
     5641 tcagattctt gctgagatta aagaagcagt agagagggcg catagtcaat ttaaaaatga
     5701 ataaaaaaga gagacttgaa aaaattagaa gattggtgac agattatcaa atcggcacgc
     5761 aagaagaaat tgtagaacat ttgaaagaag caggtatcac tgccactcag gcgacggtat
     5821 cccgagatat caaagagtta ggtattgtca aaattccttt gagagacaac acctatgtct
     5881 acgaattgcc aaaatcaatc gtaaaaagtc tgcaactggc tgaagacaat atcgaatcgg
     5941 ctgaattgat ggataagatg atcaatctcc aagttattcc aggaaatacg gcttttgtaa
     6001 aagctcagtt aatcgagact tttgcagaca agatttttag ctgtttgact gatgatagct
     6061 cgattttagt cattgccaga agtggaagtc tagcagagga aatctttgaa caagtaaaaa
     6121 attggtaggt ctatatgtta cttgaaattt cgataaaaaa ctttgccatt attgaggcta
     6181 tttccctcaa ttttgaaaag gggatgactg tcctgactgg tgaaacgggt gcagggaagt
     6241 cgattatcat tgatgccatg aatatgatgt tgggagctcg tgcgacgaca gatgttattc
     6301 gtcatggtgc accaaaggca gagattgagg ggcttttctc agttgagaat agtcgtcttt
     6361 tacaggaaat ttttgatgag caaggtttag aattaggtga tgaaattatc atccgtcggg
     6421 aaatcttgca aaatggtcgt agtatcagcc gtgtaaatgg tcagatggtt aatctgtctg
     6481 ttttgcgagc tattgggcaa catcttgtag atattcatgg tcagcatgac catgaggagt
     6541 taatgcgtcc ccaactgcat atccagatgt tggatgaatt tggtgatgcc gctttttggg
     6601 acttgaaaga aacctatcaa acgagttttg atgcctatcg gaaaatgcgc aagcaggttc
     6661 tggaagtcaa gaaaaaccaa caggaacaca aggcacgtat cgaaatgttg gaatttcaaa
     6721 tggcagagat tgaggcagca aacttgcagg ctggagaaga cttggctctc aatcaagagc
     6781 gagataaact cctcaaccac aaaaatattg cggatacact gaccaatgcc tacagtatgt
     6841 tggacaatga agatttttca agtctggcca atgttcgttc agctatgaat gacatggaaa
     6901 gtgtcgaaga gtatgatcct gaataccgtg aaatttcaag ctctctgtct gagacctact
     6961 atgttttaga agacattagc aaacgtttgg aagctatcat tgaggacctt gattttgatg
     7021 gcaatcgcct catgcaggtt gagaatcgtt tggacctcct tcatactatt actcgtaagt
     7081 atggtgggac tgttgatgat gttttgcttt attttgccaa gattacggaa gaatacaatc
     7141 tcttgacagg caataatctt tcgtctgagg acatggaagc agagcttaag aagttggaag
     7201 tcaatcttgt caatttggca ggtcaacttg cttctgctcg tcataatttg gctcagcaac
     7261 tcgaagctga gattaaacaa gaactgcaag atctttatat ggaaaaagcc cagtttcagg
     7321 ttcgttttag taagggaaaa ttcagtcgtg agggaaatga aatggttgag ttttatattt
     7381 caaccaaccc tggagaagac tttaaaccct tggttaaggt tgcttctgga ggggaattat
     7441 ctcgtctcat gttagccatt aagtctgcct tttcacgtaa agaaggcaag actagcattg
     7501 tctttgatga agtggatacg ggagtttcag gtcgtgttgc tcaagctatt gctcagaaaa
     7561 ttcataaaat tggtcagcat ggtcaggttt tggctatctc ccatttgcca caagtaattg
     7621 cgattgcaga ttatcaattc tttattgaga agattagtaa tgaccattca acggtttcga
     7681 ctgttcgtct cttgacggtc gaagagcgag tggaggaagt tgccaagatg ttggcaggtg
     7741 atgatgtgac agaagcagcc ctgacgcaag ccagagaatt gttgagaaac agggagaaat
     7801 aagatgacag attattatgt aattggagat gttcacggaa aagctgggat gctggaagac
     7861 cttcttaaaa cctgggatgg tcagacccag ttgctctttc taggtgattt gattgacaga
     7921 ggtgaggata gtcaccgtgt tcttgaaatg gtcaaggact tggtggacaa tcaaggggct
     7981 atctgtttgt ctggaaacca cgagtatatg tttttgactt ggctagatga cccagaagaa
     8041 agttatgacc attatcgtcg caatggtgga gatacaacca ttaactctat cctaggtcgt
     8101 cccttggatg caccagttga tggagtagaa gatgccaagc aggttgccgc tgaagcggca
     8161 gacttggtcg aattcattcg tcaaatgcca tttgtagtag agacagacaa gtatatcttt
     8221 gttcacgcag gtattgattt gaccttggat gactggcatg aaacaacaga ttataaaaaa
     8281 gtatggctta gaaaaccatt ccacggagct gaaaatcata ctggaaaaac cattgtcttc
     8341 ggtcatacac cggtctatgg tttgttacag caagaacgcg gtacatctga gctttggact
     8401 acagaagatg gcaagattgg aatggatgga ggagctgttt atggtggtgt ccttcacggg
     8461 attgtcttta cagaccaagg aatgacagaa caccacttta tcgaaaatga cggctttgtc
     8521 gctgaagatt agtactccta gtagggtatg gtcttgtcaa aatatcaaaa acaatttata
     8581 ataaatagat accctgaaag gaagagaatc atgaacttag aagaattgaa aaaacgacag
     8641 gagaagatcc gtaacttctc tattatcgcc catattgacc atggaaaatc aactctagca
     8701 gaccgcattt tggaaaaaac agagacggtt tcaagtcgtg aaatgcaggc ccagcttttg
     8761 gatagcatgg agctagagcg ggaacgtgga attactatta agttgaatgc catcgagttg
     8821 aattacactg caaaagatgg ggaaacttat attttccatt tgattgacac accagggcac
     8881 gttgacttta cctatgaagt ttcacgttcg ctagctgcct gtgagggtgc tattttggtg
     8941 gtcgatgcgg ctcaaggaat tgaggctcaa actcttgcca acgtttatct ggctttggat
     9001 aatgatttgg aaatcatgcc tatcattaat aaaattgacc tgccggctgc agatccggag
     9061 cgcgtgcgta cagagattga agatgtgatt ggtttggatg ccagtgaagc agttttggct
     9121 tctgccaagg ctggtattgg gattgaagaa atcctcgagc aaattgtaga aaaagtacca
     9181 gcaccaacgg gtgatgtgac ggcgccactt aaggccttga ttttcgactc tgtttacgat
     9241 gcttaccgtg gggttatcct ccaagtgcgt gtcatggacg gagtggtcaa acctggtgat
     9301 aagattcagc tcatgagcaa tagtaagacc tttgatgtgg ccgaagtcgg tatttttaca
     9361 ccaaaagcgg ttggtcgtga tttccttgcg actggtgatg ttggttacat tgcggcgtct
     9421 atcaagacgg ttcaggatac tcgtgtgggt gataccgtta ccttggcaac caatcctgcg
     9481 gcagaaccat tacatggtta taagcagatg aatcctatgg tctttgcggg tctctaccca
     9541 atcgaatcaa acaaatacaa tgacctgcgt gaagcgcttg aaaaattgca actgaatgat
     9601 gctagtcttc agtttgaacc agaaacatct caggcacttg gatttggttt ccgttgtgga
     9661 tttcttggac ttctccatat ggatgttatc caggaacgtt tagagcgtga gttcaatatt
     9721 gacctcatca tgacagctcc gtctgttatt tacaaagtta atttgaccga cggggagtca
     9781 atggatgtgt ctaacccgtc tgagttccca gatccaacca agattgcgac cattgaagag
     9841 ccttatgtca aggcgcaaat catggtaccg caagagtttg ttggagcagt gatggagcta
     9901 gctcagcgta agcgtgggga ctttgtgact atggactata ttgatgataa ccgtgttaat
     9961 gtcatctatc aaattccact ggctgaaatc gtctttgact tctttgataa acttaagtct
    10021 tcgacacgtg gttatgcaag ttttgactac gaattgtcag aatatcgccc atctaagcta
    10081 gtgaaaatgg atatccttct caacggagac aaggtggatg ccctcagctt tatcgttcat
    10141 aaggactttg cttatgaacg tgggaaactc atcgttgata agctcaagaa aatcatccct
    10201 cgccaacaat ttgaagttcc aatccaagcg gctattggac acaagattgt cgctcgtact
    10261 gatatcaagg cccttcgtaa gaacgtactt gctaaatgtt atggtggtga cgtttctcgt
    10321 aagcgtaaac tccttgaaaa acaaaaagct ggtaagaaac gcatgaaatc catcggatca
    10381 gttgaagttc cgcaagaagc cttcctcagc gtcttgagta tggatgaaga ataagttatc
    10441 agaaatgcct ttccttgttc acaggaaatt tatataagaa tcaatagtaa agcaggctta
    10501 gatggtctgc ttttttagtt tttatactct tcgaaaatct cttcaaacca cgtcagcgtc
    10561 gccttaccgt atgtatggtt actgacttcg tcagtttcat ctacaa
//
LOCUS       DAVLVP010000061        10552 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.061, whole genome shotgun sequence.
ACCESSION   DAVLVP010000061 DAVLVP010000000
VERSION     DAVLVP010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10552)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10552)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10552
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.061"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(649..765)
                     /locus_tag="VYN69_001471"
     CDS             complement(649..765)
                     /locus_tag="VYN69_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000233180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein CinA"
                     /protein_id="HEV1533725.1"
                     /translation="MVGVFRQQALYPATATSHYPSKSTAGLQELVRGGQENV"
     gene            complement(980..1333)
                     /locus_tag="VYN69_001472"
     CDS             complement(980..1333)
                     /locus_tag="VYN69_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family transposase"
                     /protein_id="HEV1533726.1"
                     /translation="MTVRHPGISPTNDLVAKKIFSNPEITCQFIRDMLDLPAQNVTIL
                     EGSNIHVLLSMPYSVQDFYTSIDVLAELDNGTQVIIEIQVHHQNFFINHLWAYLCSQV
                     NQNLEKIRQREGDTH"
     gene            complement(1451..2419)
                     /locus_tag="VYN69_001473"
     CDS             complement(1451..2419)
                     /locus_tag="VYN69_001473"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006149122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEV1533727.1"
                     /translation="MSFSDLKLFALSSNKELAERVAQEIGIELGKSSVRQFSDGEIQV
                     NIEESIRGKHVFILQSTSSPVNDNLLEILIMVDALKRASAESVNVVMPYYGYARQDRK
                     ARAREPITSKLVANMLEVAGVDRLLTIDLHAAQIQGFFDIPVDHLMGAPLIADYFERR
                     GMVGSDYVVVSPDHGGVTRARKLAEFLKTSIAIIDKRRSVDKMNTSEVMNIIGKVEGK
                     TCILIDDMIDTAGTICHAADALAEAGAVEVYASCTHPVLSGPATDNIQKSAIKKLVVL
                     DTIYLPEERLIDKIEQISIAHLLGDAIVRIHEKRPLSPLFDIEKKI"
     gene            complement(2564..3124)
                     /locus_tag="VYN69_001474"
     CDS             complement(2564..3124)
                     /locus_tag="VYN69_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HEV1533728.1"
                     /translation="MSNFLGSVHLLGVLFPDSTFLNAFESAIVAPLVEESLKLLPLVF
                     VLALIPVRKLKSLFLLGIASGLGFQMIEDIGYIHTDLPEGFDFTISRILERIISGIAS
                     HWTFSGLAVVGVYLLYRAYKGQKVGKKQGLIFLGLALGTHFLFNSPFVELETELPLAI
                     PVVTAIALYGFYHAYCFVEKHNELMT"
     gene            complement(3130..4481)
                     /locus_tag="VYN69_001475"
     CDS             complement(join(3130..4040,4040..4481))
                     /locus_tag="VYN69_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611513.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEV1533729.1"
                     /translation="MKLSYEDKVQIYELRKQGQSFKQLSKRFGVDVSGLKYMVKLIDR
                     YGIEIVKKGKNRHYSSKLKQEMMDKALLEGCSQRSISLDYALPNQGMLSFWPAQYKKN
                     GYTIVEKTRGRPAKMGRKRKKTWEEMTELERLQEENERLRTEVAYPKKVKRVRGKGRS
                     PRARKAETVREMVSGGFRLDFLLETARLARSTYYYQLKQLDGVDKDKEIKTEIQGIDN
                     EHKGNYGYRRIHLELRNRGFVVNHKKVQRLMRILGLTARIRRKRKYSSYQGEIGKKAE
                     NLIQRQFEASRPMEKCYTDVTEFAIPNSTQKLYLSPVLDGFNSEIIAYHLSTSPNLEQ
                     VKSMLEQAFTEKYYENTILHSDQGWQYQHDSYHRFLESKGIQASMSRKGNSPDNSMME
                     SFFGILKSEMFYGYEKNFRSLENLEQAIVDYIDYYNNKRIKVKLKGLSSVQYRTKSFG
                     "
     gene            complement(4478..4822)
                     /locus_tag="VYN69_001476"
     CDS             complement(4478..4822)
                     /locus_tag="VYN69_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533730.1"
                     /translation="MYEEKQYFIFILLLCIGLQYETIYYTDGSRSGAEYGLMGVSIFL
                     ALFYMIPALYFLFRIGKKWELPKKVLILSLLGGMFLSGWLSSFANTYIHDFMNCTPKV
                     RQKKSNFWGVLL"
     gene            complement(4843..5340)
                     /locus_tag="VYN69_001477"
     CDS             complement(4843..5340)
                     /locus_tag="VYN69_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007285146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HEV1533731.1"
                     /translation="MSYFEQFMQANQAYVALHGQLNLPLKPKTRVAIVTCMDSRLHVA
                     QALGLALGDAHILRNAGGRVTEDMIRSLVISQQQMGTREIVVLHHTDCGAQTFENEPF
                     QEYLKEELGVDVSDQDFLPFQDIEESVREDMQLLIESPLIPDDVIISGAIYNVDTGSM
                     TVVEL"
     gene            complement(5413..6774)
                     /gene="radA"
                     /locus_tag="VYN69_001478"
     CDS             complement(5413..6774)
                     /gene="radA"
                     /locus_tag="VYN69_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEV1533732.1"
                     /translation="MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKN
                     ARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRVLGGGVVPGSLVLIGGDPGIGKS
                     TLLLQVSTQLSQVGTVLYISGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
                     RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEG
                     TLAGPRMLEHMVDTVLYFEGERHHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNP
                     SQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAKRTTTGLDFNRASLI
                     MAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGE
                     LGLTGEIRRVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKK
                     VFA"
     gene            complement(6788..7303)
                     /locus_tag="VYN69_001479"
     CDS             complement(6788..7303)
                     /locus_tag="VYN69_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1533733.1"
                     /translation="MKIIFVRHGEPDYRELEERSYIGFGIDLAPLSEMGRQQVQKLSK
                     TPLLSSAEIIVSSAVTRALETASYVVCATGLPLRVEPLLHEWQVYKTGIENFETARRL
                     FLENKGELLPNSPIQYETATEMKSRFLECMSKYREHQTVVVVAHRMLMRQFVPNEKID
                     FCQVIECELEI"
     gene            complement(7305..7748)
                     /locus_tag="VYN69_001480"
     CDS             complement(7305..7748)
                     /locus_tag="VYN69_001480"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEV1533734.1"
                     /translation="MKIRGFELVSSFTDENLLPKRETAHAAGYDLKVAVRTVVAPGEI
                     VLVPTGVKAYMQPTEVLYLYDRSSNSRKKGLVLINSVGVIDGDYYGNPGNEGHIFAQM
                     KNITDQEVVLEVGERIVQAVFATFLIADGDAADGVRTGGFGSTGH"
     gene            complement(7805..7902)
                     /gene="ffs"
                     /locus_tag="VYN69_001481"
     ncRNA           complement(7805..7902)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VYN69_001481"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(7935..8402)
                     /locus_tag="VYN69_001482"
     CDS             complement(7935..8402)
                     /locus_tag="VYN69_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HEV1533735.1"
                     /translation="MYYTLEEKEVFMREALREAEIALEHDEIPIGCVIVKDGEIIGRG
                     HNAREELQRAVMHAEIMAIEDANLSEESWRLLDCTLFVTIEPCVMCSGAIGLARIPNV
                     VYGAKNQKFGAAGSLYDILTDERLNHRVEVEIGILEDECAAIMQDFFRNRRKK"
     gene            complement(8603..9889)
                     /locus_tag="VYN69_001483"
     CDS             complement(8603..9889)
                     /locus_tag="VYN69_001483"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011017279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEV1533736.1"
                     /translation="MTSVVVVGTQWGDEGKGKITDFLSANAEVIARYQGGDNAGHTIV
                     IDGKKFKLHLIPSGIFFPEKISVIGNGMVVNPKSLVKELSYLHEEGVTTDNLRISDRA
                     HVILPYHIELDRLQEEAKGDNKIGTTIKGIGPAYMDKAARVGIRIADLLDKDIFRERL
                     ERNLAEKNRLFEKLYDSKAIVFDDIFEEYYEYGQQIKKYVIDTSVILNDALDNGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKIDKVVGVCKAYTSRVGDGP
                     FPTELFDEVGERIREVGHEYGTTTGRPRRVGWFDSVVMRHSRRVSGITNLSLNSIDVL
                     SGLDTVKICVAYDLDGQRIDYYPASLEQLKRCKPIYEELPGWSEDITGVRNLEDLPEN
                     ARNYVRRVSELVGVRISTFSVGPGREQTNILESVWS"
     gene            complement(10120..10356)
                     /locus_tag="VYN69_001484"
     CDS             complement(10120..10356)
                     /locus_tag="VYN69_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecX"
                     /protein_id="HEV1533737.1"
                     /translation="MLQKIYEQMANFYDSIEEEYGPTFGDNFDWEHVHFKFLIYYLVR
                     YGIGCRRDFIVYHYRVAYRLYLEKLVMNRGFISC"
BASE COUNT         3257 a         2496 c         1726 g         3073 t
ORIGIN      
        1 atagagttgt ttcacatctt atttcaactc actatagaag gaggaaaagg agggctgggt
       61 ggctaacttc attatagaac tttcaaaaag atgaaaaaat agcgtcaaag aggctttttt
      121 ctctttaaaa caggaaattt caaagacaat cttgcgacta tccaaactgt acgaatagcg
      181 agacataact tacttcgcta atactatagt agattgaaac tagaatagta cacctcgatt
      241 tctaaaacat tgttagaaat cgatttgact gtcctgaacg atttgtcctg ttattatttc
      301 attttactat atcttataaa aagatagaaa tgtcaagcaa aaatgacaag tattttgata
      361 ataaacttgc catcccatca actttctact atataaagta tgaactcata gcaaggactc
      421 aaactcagcc acggtcatgc ctaattgctc actcgcaacc tcaggtgtta gaagaccttg
      481 acgtactaga tttactaaac ctacttttaa tccctcttca aggccttctt ctcgtccttg
      541 ttcacaaccc tctgcacgac cacgttcaat accacgctct agtccttgtt caataccttc
      601 gcatagtcct gtgctaacaa gacttgttct tctcgcatat gtagttgact aaacattttc
      661 ctgtcctcct cggaccagct cttgtagtcc agcagttgat ttgcttgggt aatggctcgt
      721 tgcggttgct gggtaaaggg cttgttgccg aaaaactcca accatggctt gcgaaccttg
      781 tctttgctgg tttctctgta ttttttaatt ccaagaatgc catcttaacc agatggtttt
      841 cctgtccatt gttggtaatc gccaatacct cacctgttgt gtcttcgcgc atactaacgc
      901 tatgaaaagc caggccatct gagaaataat tgctatccac gatagcaatg acgtaaacag
      961 gagcgatgtg tttgtagatc tagtgagtat caccttctcg ctgacgaatt ttttcaagat
     1021 tttgattaac ctgactgcac aggtaagccc acaagtgatt gatgaaaaaa ttctgatgat
     1081 ggacttgaat ctcaataatt acttgagtac cgttatccaa ctccgccaag acgtctatac
     1141 tggtataaaa atcctgcacc gagtaaggca tggagagtaa tacgtgaata ttgctaccct
     1201 ccaaaatggt cacattttgg gctggcaaat ccagcatatc gcgaataaat tgacaagtaa
     1261 tttctggatt gctaaagatt ttcttagcta ccaagtcatt ggttgggcta atgcccggat
     1321 gacgtacagt catatttctt ctctttttat tatatcacag ttttaaatct agctttacta
     1381 gattcaccgc tactatctat ttattcggaa aaaagacgaa aaaacctgag aatcatctca
     1441 ggcttggtca ttaaattttt ttctcaatat cgaaaagtgg agaaagtggt cgtttttcat
     1501 gaatacgtac gatagcatcc cctaggagat gagcgattga aatctgctca atcttatcaa
     1561 tcaaacgctc ttctggcaga tagatggtat ccaaaacaac caatttctta atagctgatt
     1621 tttggatatt gtccgtagca ggaccagaaa gaactgggtg cgtacagctt gcatagactt
     1681 caacagcacc agcttccgca agagcatctg ccgcatgaca aatcgttcca gcggtatcaa
     1741 tcatatcatc aatcaagata caagtcttgc cttcaacctt accgatgatg ttcataactt
     1801 cactagtatt catcttatca acgctacgac gtttatcaat aatagcgata gaagttttca
     1861 aaaattctgc caacttacga gcacgagtca cccctccatg gtccgggctg acaaccacat
     1921 agtcagaacc aaccatacca cgacgctcaa aataatctgc aatcagagga gcacccatca
     1981 aatgatccac aggaatatca aagaatcctt gaatttgcgc agcatgcaag tcgatggtca
     2041 ataaacgatc cactccagct acttcaagca tatttgcgac aagttttgaa gtgattggct
     2101 cacgcgctct cgcctttcta tcctgacgtg catacccata gtaaggcatg acaacattga
     2161 cagattctgc actcgcacgc ttcaaagcat ctaccataat caaaatttca agcagattgt
     2221 catttacagg cgaactagtt gattgtagga taaagacgtg tttcccacgg attgattctt
     2281 caatgttgac ctgaatctct ccatctgaaa attggcgaac acttgatttc cccaactcta
     2341 tcccaatctc ctgcgccaca cgttctgcca attctttatt agaagaaagg gcaaatagct
     2401 ttaaatcaga aaaagacatg atttcctccg gtatatatgt ataacttgtg cttttcacaa
     2461 gattttccat ctaccattgt agcgcttttt gcactatttt tcaatcaaaa ataaaagaag
     2521 ggcaccatat ttgtaccctt gcatcattct tttgaaaaat attctaggtc atcaactcat
     2581 tgtgtttctc aacaaagcaa taagcatgat aaaaaccata gagagcaata gccgtaacca
     2641 ctggaatcgc taaaggcaac tctgtttcca actccacaaa aggagagtta aacaagaagt
     2701 gagttcccaa ggctaaacct agaaaaataa ggccctgttt cttgccaacc ttctgtcctt
     2761 tataggctct gtaaagcaag taaacaccta ctacagctag acctgaaaaa gtccagtgag
     2821 aggcaattcc tgagatgata cgctctaaaa ttcgcgaaat agtaaagtca aagccctctg
     2881 gcaaatccgt atgaatataa ccaatatcct caatcatttg gaatcccaaa ccggaagcaa
     2941 ttccaagtaa aaacaaagat tttaattttc gcacaggaat caaagccaaa acaaaaacaa
     3001 gtggcaataa tttcaaggat tcttctacca aaggagccac aatagcactt tcaaaggcat
     3061 ttaaaaatgt actatctggg aaaagaaccc ccagtaaatg tactgacccc aaaaagttag
     3121 acaaataatt tatccaaagg atttagttct gtactgcaca gaactaagtc cttttagttt
     3181 taccttaatt cgtttgttgt tgtagtaatc aatgtagtcc acaatagctt gttcaaggtt
     3241 ttctaaagat ctaaagttct tctcatagcc ataaaacatt tcggatttta aaatgccaaa
     3301 gaaagattcc atcatgctgt tgtctgggct gttgcccttg cgtgacatgg atgcttgaat
     3361 tcccttactc tctaggaacc gatgataaga atcgtgttgg tattgccagc cttggtcact
     3421 atggagaatc gtattctcat agtatttctc tgtaaaggcc tgttctaaca tactcttcac
     3481 ttgttctaag tttggcgaag tagaaagatg ataagcaatg atttcactgt taaaaccatc
     3541 taaaactggt gataaataga gtttctgtgt gctatttgga atggcaaact ctgtcacatc
     3601 tgtatagcac ttttccattg gcctcgatgc ttcgaactgg cgttgaatga gattctctgc
     3661 tttcttgcca atctctcctt ggtaggaaga atacttccgt ttacggcgaa ttcgagccgt
     3721 taaaccaaga attctcatca accgttgaac tttcttatga ttgaccacaa aaccacgatt
     3781 ccttagttct aaatggattc gacgatagcc atagttgcct ttatgttcat tatcaatgcc
     3841 ctgaatttcg gttttaatct ctttatcttt atcaacccca tccagttgtt tcaactgata
     3901 atagtaagtc gagcgagcta aacgtgctgt ttcaagaaga aaatctagtc gaaatcctcc
     3961 tgaaaccatt tctctaactg tctctgcctt tctcgctcta gggcttcgtc cctttcctct
     4021 aactctttta acttttttag gtaggccacc tcagtccgta agcgttcatt ctcctcctgg
     4081 agtcgttcta gttctgtcat ttcttcccaa gtcttcttcc gtttacgtcc cattttagct
     4141 ggtctccctc ttgttttctc aacaatagta tacccgtttt tcttgtattg tgccggccaa
     4201 aatgaaagca ttccttggtt tgggagggca taatcaagac taatacttct ttgtgaacaa
     4261 ccttccagta aggctttatc catcatttct tgtttcaatt tagaagaata gtgacgattc
     4321 ttcccttttt taacgatttc tatgccataa cggtcaatta atttcaccat gtactttaga
     4381 ccagaaacat ccacaccaaa tctttttgaa agctgtttga agctttgtcc ttgctttctt
     4441 agttcataga tctgaacttt atcttcataa ctcaatttca taataaaaca ccccaaaagt
     4501 tagatttttt ctgtctaact tttggggtgc agttcataaa atcatggata taagtattag
     4561 caaaactaga caaccagcct gaaaggaaca tccctcccaa taaagacaga atcaaaacct
     4621 tctttggcaa ttcccatttt ttcccaatac ggaagagaaa ataaagagcc ggaatcatgt
     4681 aaaagagagc tagaaagata gaaactccca ttagtccata ttccgcacct gacctcgaac
     4741 cgtccgtata gtagatggtt tcatactgta aaccaataca tagcaataaa ataaaaataa
     4801 aatactgctt ttcttcatac actttctttc taaatgaagt atttataatt ctacgactgt
     4861 catacttcct gtatcaacat tgtaaatagc accagagata atgacatcgt ctggtattag
     4921 gggagactcg ataagcagtt gcatatcctc gcgtacactc tcttctatat cttggaaggg
     4981 caagaagtcc tggtctgaca catctacacc taattcctct tttaaatact cctgaaaagg
     5041 ttcattttca aaggtctgag caccacagtc tgtatggtgc aataccacaa tctctcttgt
     5101 ccccatttgt tgctgggaaa taactagcga acgaatcatg tcttcagtca ctcgaccacc
     5161 tgcattccgc aagatatgag catccccaag tgccaaacct agagcttgcg caacgtgcaa
     5221 acgtgagtcc atacaggtca caatagctac tcttgttttg ggtttaagtg gcagatttaa
     5281 ctgcccatgt agggcaacat aagcctgatt agcttgcata aactgttcaa aatacgacac
     5341 gattccctcc ttgaaaattt gatagtcaaa tatttctcct atcttatcat ttttaagaga
     5401 atttgtcacg gattatgcaa agaccttttt caaaacttcc tgaatcgttg tcacgccaat
     5461 gacctgaatt tccttaggca gagtgattcc tgtcaaggaa ttcttaggta cataaatctt
     5521 agtaaagccc agtttagcag cttcgttgat gcgttgctca atacgattca cgcgccgaat
     5581 ctctcctgtc aagcccagtt ctccgacaaa acattcctga ggattagttg gcttgtcttt
     5641 gtagctcgaa gcaatagcaa ctgcaacagc caagtcaatg gcaggttcat ccaatttaac
     5701 accaccagca gatttgagat aggcatcctg attttgcaag agaagccctg cccgtttttc
     5761 caaaacagcc ataatcaagc tagcacggtt aaaatcaagt cctgtcgtag tacgcttggc
     5821 atttccaaac atggtcggtg ttaccaaagc ctgaacctcc gccaaaatcg gacgcgtccc
     5881 ttccatggtt acaacgatgg aggaaccagt tgccccatcc aaacgctctt ctaggaaaac
     5941 ttgactcgga ttgagtacct caaccaagcc gcccgactgc atctcaaaaa tcccaatctc
     6001 attagtggaa ccaaaacgat ttttgaccgc tctcaaaata cgaaaggtgt ggtgacgctc
     6061 cccttcaaag taaagcaccg tatccaccat atgctccaac atacgaggcc cagccaaggt
     6121 tccttctttg gtcacatgac ctacgataaa gatggcaatg ttattggtct tggccaactg
     6181 catgagttca gcggtcactt cacgtacctg agaaacagac ccctgcaccc ctgaaatctc
     6241 aggagacatg atggtctgga tggaatcaat aataagaaag tctggctgga tacgctccac
     6301 ttctgcacga acactctgca tattggtctc tgcatagaga taaaactcac tatcaatatc
     6361 acctaagcgc tctgcacgta gtttaatctg ctgggcagac tcctccccac tgatatagag
     6421 aactgtcccc acttgggaca actgggttga gacttgtagg agaagagttg atttcccaat
     6481 cccaggatcc ccaccgatga ggacgagact tcctggtacc actccgcctc caagcacacg
     6541 gttgaattcc tccatctccg tcttggttcg attgacattg atggaagtca cctcagctag
     6601 tttcatgggc ttggttttct cacctgtcaa ggacacacgc gcattcttga cctcggcaac
     6661 ctcaacctct tccacaaaag aagaccaaga cccacagttg gggcaacgtc ccagatattt
     6721 aggggaatta tacccacaat tttgacatac aaatgtcgct tttttctttg cgatgacaaa
     6781 cctctttcta tatctctaac tcacactcaa tcacttggca aaaatcaatc ttctcatttg
     6841 gcacaaactg gcgcatgagc attcgatgag caacaactac cacagtctga tgttctcgat
     6901 acttagacat acattctaga aaccgagact tcatttccgt agctgtctca tattgaatag
     6961 gactattagg aagcaactcc cccttgtttt ctaaaaacag tcttctagct gtttcaaagt
     7021 tttctattcc tgttttatag acctgccatt catgtaataa aggctctact cttaaaggaa
     7081 gacccgtagc acagaccaca tacgaagccg tttctaaagc tcttgtgact gcagaagata
     7141 cgattatttc agctgacgag agtaaaggag ttttgctcaa tttctggact tgctgccgtc
     7201 ccatctcaga caagggtgcc aaatctatcc caaatcctat ataagaacgc tcctctaact
     7261 cacggtaatc tggctcccca tgacgtacaa agataatctt cattctagtg ccctgtcgat
     7321 ccaaatccac cagttcgaac gccatcagct gcatctccat ctgcaattaa gaaagtagca
     7381 aaaacagcct ggacaatacg ctccccaact tcaagaacaa cctcttggtc tgtgatattc
     7441 ttcatctgcg caaaaatatg cccttcattt ccaggatttc cataataatc cccatcaatg
     7501 accccaactg agttaattaa aaccaagccc ttcttacgag aatttgaaga acgatcatag
     7561 aggtagagaa cctcagtcgg ctgcatataa gccttaaccc ctgtcggaac caagacaatc
     7621 tctcctggcg caacaactgt acgcacagca acctttaagt cgtaaccagc cgcatgcgct
     7681 gtctcacgct tgggcaataa attttcatct gtaaaactcg aaaccaattc aaaaccacga
     7741 attttcataa ttttctcttt tctattatca tttattctag attattctat cttatttatt
     7801 cggaaaaagc acgaaaaaaa gagcacacaa ttcaaatcgc ttagggctgc tggattcctc
     7861 ccctgacccg cttcacgcag aactgttgct ccactattta ttatatcaca ttcctattca
     7921 ttttaaaagc aaaattattt tttccgtcta tttctaaaaa aatcctgcat gatagctgcg
     7981 cattcatctt ccaaaattcc tatttcaacc tccacacgat gattgagacg ctcatctgtc
     8041 aagatatcgt acaaactccc agcagcgcca aatttctggt ttttagcccc atagaccaca
     8101 tttggaatgc gggcaagccc aatcgcccca ctacacatga cacaaggttc aatggtcaca
     8161 aaaagtgtgc aatccagcaa gcgccagctc tcctcactca agttcgcatc ctctatagcc
     8221 ataatttccg catgcataac cgctcgctgt aattcctcac gcgcattatg cccacgacca
     8281 atgatttccc catctttgac aatcacacaa ccaattggaa tttcatcgtg ttcaagagca
     8341 atctcagcct ctctcaaagc ctccctcata aagacttctt tttcttcaag cgtataatac
     8401 atctcttttc tcttttccta cttatcgatt ttattattat atcatgaatc ccaacacaaa
     8461 aaaagccacc gaatgcggtg actctatagg gagattatta tgaaaaagaa aagtttagga
     8521 tatttgttac aacaagttag gaggtcttct tgtaaccgtc tatagtatac ccgacctatc
     8581 ttaaacaaat cttaaaaatc tcttaggacc aaacactttc taaaatattt gtttgttcac
     8641 gaccaggacc tactgagaaa gtagaaatac gaacgccaac caattcactc acacgacgaa
     8701 catagttacg cgcattctca ggaagatctt ccaaattgcg aactccggta atatcttctg
     8761 accaacctgg caactcttca tagataggct tgcaacgttt caattgctca agactagctg
     8821 gatagtagtc aatacgttga ccgtcaagat cataggccac acagattttc acagtatcca
     8881 aaccgctcaa aacatcaata gagttcaaag aaaggttagt aataccagaa acacgacggc
     8941 tatgacgcat cacaactgag tcaaaccaac ctacacgacg tggacgacca gttgttgtac
     9001 catattcatg acccacttca cggatacgtt ctcccacttc atcaaacaac tcagttggga
     9061 aaggaccatc tcctacacga ctcgtataag ctttacatac acctacaacc ttgtcaatct
     9121 tgcttggacc gacaccagaa ccaattgtca caccaccagc tacagggttt gatgacgtaa
     9181 caaatggata agtaccttgg tcgatatcta gcataacacc ttgtgcacct tcaaaaagca
     9241 cacgtttgcc attatcaagc gcatcattca agataacaga tgtatctatc acgtatttct
     9301 tgatttgttg accatattcg taatattctt caaaaatatc atcgaaaaca atcgctttac
     9361 tgtcatacaa tttttcaaaa agacgattct tttcagcaag gttacgttct aaacgctcac
     9421 ggaaaatatc tttatctaaa agatctgcaa tacgaattcc aacacgagca gccttgtcca
     9481 tataagctgg accaattccc ttaattgtcg taccaatctt attgtcgccc ttagcttctt
     9541 cttgcaagcg atccaactcg atatgataag gcaaaataac atgcgcacga tcagaaatac
     9601 gcaagttatc agttgttaca ccttcctcat gaagatagct caactctttt acaagagatt
     9661 taggatttac aaccatacca ttcccaatga cagatatttt ttcagggaag aaaatcccag
     9721 atggaatcaa gtgcaactta aatttcttac cgtcaatcac aatcgtgtga ccagcattat
     9781 caccaccttg gtaacgtgca atcacttctg cattcgctga aaggaagtct gtaatcttcc
     9841 ctttaccttc atcaccccat tgggtaccta caacaacaac tgaagtcata attttgtctg
     9901 agccctcagg ctcttccttt ctcacataca tggcaggact ctcacctgca attatatctt
     9961 acaatttatt ataataaaaa atcgcctttt tatcaagaag aaacaataga aagatttgct
    10021 atttccaact attaaaaaat gatttagaaa aattactagc tatttactat tatctttaca
    10081 taaaagagta aattagttcg gaaatttact aaaattacct caacaagaaa taaaaccccg
    10141 attcattacc aatttttcaa gatacaaacg ataagcaaca cgataatggt aaacgataaa
    10201 atccctacga caaccaatgc catatctcac taaataataa attaaaaatt taaaatgaac
    10261 atgttcccag tcaaaattat caccaaatgt aggaccatac tcttcttcaa tactatcata
    10321 gaaattagcc atctgctcat aaattttttg taacataatc aaatactcct tttctttttt
    10381 ataaacttat tctaacaaaa aattttacac attcactatc aattcctgaa ttgttaaaat
    10441 accaggctca acaagataaa aataggaaaa gttgacagaa tagaaacaaa tttgctacct
    10501 aaatttcaaa cgattaaagt tctaaaaaga ccgccttaaa acttttgggt aa
//
LOCUS       DAVLVP010000062        10501 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.062, whole genome shotgun sequence.
ACCESSION   DAVLVP010000062 DAVLVP010000000
VERSION     DAVLVP010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10501
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.062"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(31..1455)
                     /locus_tag="VYN69_001485"
     CDS             complement(31..1455)
                     /locus_tag="VYN69_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent glyceraldehyde-3-phosphate
                     dehydrogenase"
                     /protein_id="HEV1533738.1"
                     /translation="MTRYQNLVNGKWKSSEQEITIYSPINQEELGTVPAMTQTEADEA
                     MQAARAALPAWRALSAIERAAYLHKTAAILERDKEKIGTILAKEVAKGIKAAIGEVVR
                     TADLIRYAAEEGLRITGQAMEGGGFEAASKNKLAVVRREPVGIVLAIAPFNYPVNLSA
                     SKIAPALIAGNVVMFKPPTQGSISGLLLAKAFEEAGIPAGVFNTITGRGSEIGDYIIE
                     HKEVNFINFTGSTPIGERIGRLAGMRPIMLELGGKDAALVLEDADLEHAAKQIVAGAF
                     SYSGQRCTAIKRVIVLESVADKLATLLQEEVSKLTVGDPFDNADITPVIDNASADFIW
                     GLIEDAQEKEAQALTPIKREGNLLWPVLFDQVTKDMKVAWEEPFGPVLPIIRVASVEE
                     AIAFANESEFGLQSSVFTNDFKKAFEIAEKLEVGTVHINNKTQRGPDNFPFLGVKGSG
                     AGVQGIKYSIEAMTNVKSIVFDVK"
     gene            complement(2000..4279)
                     /gene="pulA"
                     /locus_tag="VYN69_001486"
     CDS             complement(2000..4279)
                     /gene="pulA"
                     /locus_tag="VYN69_001486"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000283081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HEV1533739.1"
                     /translation="MYNYPMRIHYHRKNGEYDTCSFVKSQDQRIDLLTYKEDYFGALF
                     SFEHPSSHVIESLNFVVHTGQTSKEYSIRFNHYPLLTEVWILEGDDRIYYSENPAIAS
                     PFYKNQNPFAFDKAINSASFDHHWGYQGELGCRVEDNQAHFSLWSPTATEVQVVVYES
                     AANDAPVWKTFEMKRGNSYSYNHKDNTIGVWSLDVEEDLVGKTYQYQVQFPHHQTLTR
                     DPYTIATSPDGKRSAILSHVEKQVENFEVKHGSEATWRLENPCKAVICEMHIRDLTKS
                     PTSGVDEHLRGTFLGAAQAGTVNQYGQSTAFDYIKKLGYNYVQLQPIADRHKEYDEDG
                     NVTYNWGYDPQNYNAPETSFSTNPDDPAQVIRDLKVMVQAYHDAGIGVIMDVVYNHTF
                     SVVDAPFQTTVPDYYYRMNPDGTFQNGTGVGNETASEHEMFRKYMIDSLLYWVQEYNI
                     DGFRFDLMGIHDVKTMQMIRQSLDEIDSNIILYGEGWDMGTGLTPYDKAKKDNAYQMP
                     NIGFFNDNQRDAVKGGEVYGAIKSGFVSGAATEPILAKAILGSRELGTYTHPNQVLNY
                     VEAHDNYNLHDLLATLHPDQSSEQIMRKVETATAMNLLMQGMAFMEIGQEFGRTKLVA
                     TGENGELTHDDRERAMNSYNAPDSVNQVNWNLINERQDSIEFIRQVIRLKTKTGAFSY
                     SSYDEIYHHVFVHSAIEHSGCLIYEVHGKEHLLVVVNAKSEPYQFENAGNLAMLVTNS
                     RSKEDNVLNDISLAVLSVL"
     gene            complement(4391..6349)
                     /gene="ligA"
                     /locus_tag="VYN69_001487"
     CDS             complement(4391..6349)
                     /gene="ligA"
                     /locus_tag="VYN69_001487"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001042597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEV1533740.1"
                     /translation="MNKRMNELVALLNRYATEYYTSDNPSVSDSEYDRLYRELVELET
                     AYPEQVLADSPTHRVGGKVLDGFEKYSHQYPLYSLQDAFSREELDAFDARVRKEVAHP
                     TYICELKIDGLSISLTYEKGILVAGVTRGDGSIGENITENLKRVKDIPLTLPEELDIT
                     VRGECYMPRASFDQVNQVRQENGEPEFANPRNAAAGTLRQLDTAVVAKRNLATFLYQE
                     ASPSTRDSQEKGLKYLEQLGFVVNPKRILAENIDEIWNFIQEVGQERENLPYDIDGVV
                     IKVNDLASQEELGFTVKAPKWAVAYKFPAEEKEAQLLSVDWTVGRTGVVTPTANLTPV
                     QLAGTTVSRATLHNVDYIAEKDIRKDDTVIVYKAGDIIPAVLRVVESKRVSEEKLDIP
                     TNCPSCNSDLLHFEDEVALRCINPRCPAQIMEGLIHFASRDAMNITGLGPSIVEKLFA
                     ANLVKDVADIYRLQEEDFLLLEGVKEKSAAKLYQAIQASKENSAEKLLFGLGIRHVGS
                     KASQLLLQYFHSIENLYQADSEEVASIESLGGVIAKSLQTYFATEGSEILLRELKETG
                     VNLDYKGQTVVADAALSGLTVVLTGKLERLKRSEAKSKLESLGAKVTGSVSKKTDLVV
                     VGADAGSKLQKAQELGIQVRDEAWLESL"
     gene            complement(6442..7620)
                     /locus_tag="VYN69_001488"
     CDS             complement(6442..7620)
                     /locus_tag="VYN69_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1533741.1"
                     /translation="MNRYAVQLISRGAINKMGNMLYDYGNSVWLASMGTIGQTVLGMY
                     QISELVTSILVNPFGGVISDRFSRRKILMTADLVCGILCLAISFIRNDSWMIGALIVA
                     NIVQAIAFAFSRTANKAIITEVVEKDEIVIYNSRLELVLQVVGVSSPVLSFLVLQFAS
                     LHMTLLLDSLTFFIAFVLVAFLPKEEAKVQEKKAFTGRDIFVDIKDGLHYIWHQQEIF
                     FLLLVASSVNFFFAAFEFLLPFSNQLYGSEGAYASILTMGAIGSIIGALLASKIKANI
                     YNLLILLALTGVGVFMMGLPLPTFLSFSGNLVCELFMTIFNIHFFTQVQTKVESEFLG
                     RVLSTIFTLAILFMPIAKGFMTVLPSVHLSSFLIIGSGVIILSCISFIYVRTHFEKLI
                     "
     gene            complement(7700..8563)
                     /locus_tag="VYN69_001489"
     CDS             complement(7700..8563)
                     /locus_tag="VYN69_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012291596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1533742.1"
                     /translation="MEHLGKVFREFRTSGNYSLKEAAGESCSTSQLSRFELGESDLAV
                     SRFFEILDNIHVTIENFMDKARNFHNHEHVSMMAQIIPLYYSNDIAGFQKLQREQLEK
                     SKSSTTPLYFELNWILLQGLICQRDASYDMKQDDLDKVADYLFKTEEWTMYELILFGN
                     LYSFYDVDYVTRIGREVMEREEFYQEISRHKRLVLILALNCYQHCLEHSSFYNANYFE
                     AYTEKIIDKGIKLYERNVFHYLKGFALYQKGQCKEGCKQMQEAIHIFDVLGLPEQVAY
                     YQEHYEKFVKS"
     gene            complement(8566..10467)
                     /locus_tag="VYN69_001490"
     CDS             complement(8566..10467)
                     /locus_tag="VYN69_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000584877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEV1533743.1"
                     /translation="MIILQANKIERSFAGEVLFDNINLQVDERDRIALVGKNGAGKST
                     LLKILVGEEEPTSGEINKKKDISLSYLAQDSRFESENTIYDEMLHVFNNLRRTERQLR
                     QMELEMGEKSGEDLDKLMSDYDRLSENFRQAGGFTYEADIRAILNGFKFDESMWQMKI
                     AELSGGQNTRLALAKMLLEKPNLLVLDEPTNHLDIETIAWLENYLVNYSGALIIVSHD
                     RYFLDKVATITLDLTKHSLDRYVGNYSRFVELKEQKLVTEAKNYEKQQKEIAALEDFV
                     NRNLVRASTTKRAQSRRKQLEKMERLDKPEAGKKAANMTFQSEKTSGNVVLTVENTAI
                     GYDGEVLSQPINLDLRKMNAVAIVGPNGIGKSTFIKSIVDQIPFIKGEKRFGANVEVG
                     YYDQTQSKLTPSNTVLDELWNDFKLTPEVEIRNRLGAFLFSGDDVKKSVGMLSGGEKA
                     RLLLAKLSMENNNFLILDEPTNHLDIDSKEVLENALIDFDGTLLFVSHDRYFINRVAT
                     HVLELSENGSTLYLGDYDYYVEKKATAEMSQTEEASTSNQAKEASPVNDYQAQKESQK
                     EVRKLMRQIESLEAEIEELESQSQAISEQMLETNDADKLMELQAELDKISHRQEEAML
                     EWEELSDQV"
BASE COUNT         3137 a         2329 c         1901 g         3134 t
ORIGIN      
        1 accaggaaaa caatttcctg attttacacg ttatttcaca tcaaaaacaa tggatttgac
       61 atttgtcatc gcttcaatgc tatatttaat tccttgcact ccagctccag aacctttgac
      121 accaaggaat gggaaattat ctggaccacg ctgggtttta ttattaatgt ggactgtacc
      181 tacttcaagt ttttcagcaa tttcaaaggc ttttttgaaa tcatttgtaa agactgatga
      241 ttgaaggccg aattcagatt cgttggcaaa ggcaatagct tcctctacac tagccacacg
      301 aatgattggt aaaacaggac caaatggctc ttcccatgcc actttcatat cttttgtaac
      361 ttggtcaaaa agcactggcc agaggagatt gccctcacgt ttgattggtg taagagcctg
      421 agcctctttt tcttgtgcat cctcaatcaa gccccaaatg aagtcggctg aagcattgtc
      481 aataacaggt gtaatatcag cattgtcaaa tggatcacca actgttaatt tagaaacttc
      541 ttcctgaagc aaagtagcta atttatctgc tacactttcg agaacaatga cacgtttaat
      601 ggccgtgcaa cgttgtcctg agtagctaaa ggctcccgca acaatttgct tggcagcatg
      661 ttccaaatct gcatcttcta gtacaagagc tgcatctttc ccaccaagtt ccaacatgat
      721 aggacgcata ccagctaaac gaccaatacg ttctccaata ggggttgaac ctgtaaagtt
      781 gatgaagttg acttctttgt gctcaatgat ataatcccca atttctgaac cacgacctgt
      841 aatggtgttg aaaacacctg ccggaatccc tgcttcttca aatgctttag ccaacaagag
      901 tccagaaatg gaaccttgtg ttggtggctt aaacatgacc acattccctg caatcaaggc
      961 aggtgcaatt ttagaagcag ataaattaac tggataatta aagggagcaa tcgctagcac
     1021 gataccaact ggttcacgac ggacaacagc cagtttgttt ttacttgctg cctcaaaacc
     1081 accaccttcc attgcttgtc cagtgatacg gagaccttcc tcagcagcat aacgaatcaa
     1141 gtctgctgta cgcactactt ctccaattgc tgctttaatc ccttttgcta cttctttggc
     1201 aaggatagta ccaatttttt ccttatcgcg ttctaaaata gctgctgttt tatgcaaata
     1261 agccgcacgt tcaattgctg ataaagctcg ccatgctggc agggctgcac gcgcagcttg
     1321 catagcctca tcagcttcag tctgagtcat ggctggaact gtacccaatt cttcttgatt
     1381 gattggtgaa taaatcgtaa tttcttgttc agatgatttc cattttccat ttactaaatt
     1441 ctgatatctt gtcaaattct tgtcctcctg aaaatgatta agatatattt taccaaattt
     1501 tcagaaaatc acaacccttt ttgcaaaatt tttgagatta ttttcacaag cttgattttt
     1561 caaagtatac tcaataaaaa taaaaaaaat ccactacatc aaggcgagac taatgtggtt
     1621 tgaagaaatt ttcgaagagc gtgaatgagt atcatctata gtaaaataaa aaaactgaac
     1681 aatttggttg gggacagtca aaccaatttc tcacaatgtt ttagaaacaa gggtgtgcta
     1741 ttccaatttc aggctactat aactgtcata gattgctgaa acaaagtcta ggtaaaagtc
     1801 ttcataataa aaagaccccc tatcaagtgt tcaaaaactt tgataggagg tcttgttttg
     1861 tgaaaatatt tatcaaattt tctatacaag tgagctgtta gccaggttct ttctattctt
     1921 tcaatttcaa tgaatggatt ttttaccaat actcataact gggaatttgt ctgtgtaaaa
     1981 atagcgagat agatggtatt tataaaacac tcaagacagc tagactaata tcatttaaaa
     2041 cattatcttc ttttgagcga ctgttggtta ccaacatagc taaatttcct gcattttcaa
     2101 attgataggg ttctgattta gcattcacaa ctaccaagag gtgttctttg ccgtgaactt
     2161 catagataag gcagccgcta tgttcaatcg cagaatgcac aaagacatga tggtaaattt
     2221 catcatagct agagtaagaa aaggcaccag tttttgtctt caatcggatg acttgacgga
     2281 taaactcaat actgtcttga cgctcattaa tcaagttcca gttcacttgg ttcacactgt
     2341 caggagcatt atagctattc atcgcacgct ctctatcatc atgggtcaac tcaccatttt
     2401 caccagtcgc aaccagtttg gtacgaccaa attcttgacc gatttccata aaggccatcc
     2461 cctgcatgag cagattcatg gctgtggcag tttcgacctt gcgcatgatt tgctctgaac
     2521 tttggtctgg atgaagggtt gccaataaat cgtgaagatt gtaattgtca tgggcttcta
     2581 catagttaag cacctgattt ggatgtgtat aggttcctaa ttcacgactt cctaggattg
     2641 ctttagctag aattggctct gtcgcagcac cactgacaaa acctgacttg atagcaccat
     2701 aaacttctcc ccctttgaca gcatcgcgct gattgtcatt aaagaaacca atatttggca
     2761 tctggtaggc attgtccttc ttggccttat cataaggggt aagacctgtt cccatatccc
     2821 atccttctcc atagaggata atgttggagt cgatttcatc caagctttga cgaatcatct
     2881 gcatggtctt gacatcatga atccccatca agtcaaaacg gaagccgtca atattatatt
     2941 cctgcaccca gtatagaaga gaatcaatca tatacttgcg aaacatttcg tgttcactgg
     3001 ctgtttcatt tccaacaccc gttccattct ggaaggtacc atctggattc atacgataat
     3061 agtaatcagg gactgttgtt tggaatggtg catcaacaac tgagaaggta tggttataga
     3121 ctacatccat aatgactcca atacccgcat cgtgataagc ttgaaccatc accttcaaat
     3181 cacgaatgac ctgagctgga tcatctggat tagttgaaaa actagtttct ggcgcgttat
     3241 agttttgtgg atcataaccc cagttgtagg ttacatttcc atcctcatcg tattctttat
     3301 gacggtctgc aattggttgc aattgaacat aattgtagcc cagcttcttg atgtaatcaa
     3361 aagcagttga ctggccgtat tggttaactg ttccagcctg agcagcaccc aagaaagttc
     3421 ctcgaagatg ttcatctaca cccgatgtag gtgatttagt caaatcacga atgtgcattt
     3481 cacagataac tgccttacat ggattttcca agcgccaagt agcctccgaa ccgtgcttaa
     3541 cctcgaagtt ttcaacttgc ttttctacat ggctcagaat agctgaacgt ttgccatcag
     3601 ggctggtcgc gattgtataa ggatcacgtg tcagtgtttg gtgatgaggg aattggactt
     3661 gatactgata agtcttacct accaaatctt cttcaacatc caaactccag acaccgattg
     3721 tattgtcctt atgattataa gagtagctat tgcctctttt catctcaaaa gtcttccaaa
     3781 cgggtgcatc attagcagct gattcataaa cgacaacttg cacttctgtc gctgtaggtg
     3841 accagaggga aaaatgagcc tgattgtcct ctacacggca acccaattct ccttggtaac
     3901 cccaatgatg atcaaaacta gcactgttaa tggccttatc aaaggcaaaa ggattttgat
     3961 ttttatagaa aggactggca atagcaggat tttcagagta ataaatccta tcatcgcctt
     4021 ccaaaatcca gacctctgtc aataggggat agtgattaaa acggatagaa tattctttac
     4081 tagtttgacc tgtatgaacc acaaaattca agctttctat aacatgtgaa cttgggtgtt
     4141 caaagctaaa taaagctcca aaataatctt ctttgtaggt tagcaaatca attcgttgat
     4201 cctgactttt tacaaaggag caagtgtcat attctccatt cttacgatgg taatgaatgc
     4261 gcatagggta gttatacatt ttttattttt cctttttact ttgtttctat ttcactaata
     4321 aatttttgtc aatctcgtct caattaacag acatagtcat attctctaaa ctctgttttt
     4381 aaacgatcca ttacaaactt tctagccatg cctcatctct gacctggata ccaagttctt
     4441 gtgctttttg cagtttactt ccagcgtctg cacctaccac gacgaggtcg gtctttttag
     4501 aaacactacc tgtcactttg gcacccagac tttcgagttt acttttagct tctgagcgct
     4561 tgagtcgttc caattttcct gtcaatacca cggtcaaacc tgacaaggcc gcatccgcta
     4621 ctaccgtctg tcctttatag tccagattga ccccagtttc tttcaattct ctgagcagaa
     4681 tttcagagcc ttctgtcgca aaataagtct gaagactttt ggcaatcacg ccacctagac
     4741 tttcaatact agccacttcc tctgaatctg cctgatacag attttcaatt gaatggaaat
     4801 attgaagtaa aagctgacta gccttgcttc cgacatgacg aattcccaaa ccaaataaga
     4861 gcttctcggc agaattttcc tttgatgctt ggatagcctg atacagttta gcagcggact
     4921 tttccttaac cccctctaaa aggaggaaat cctcttcttg caaacgataa atatccgcca
     4981 catccttgac taaattagca gcaaaaagct tctcaacaat agatggacca aggcctgtaa
     5041 tattcatagc atcacgagaa gcaaagtgaa tcaagccttc catgatttga gcagggcaac
     5101 gcggattgat acaacgtagg gccacttcat cttcaaagtg caacaagtca gagttacaac
     5161 ttggacagtt tgtagggata tctagttttt cttcagaaac ccgtttggac tctaccacac
     5221 gtaaaacggc agggatgatg tcaccagcct tatatacaat gaccgtatcg tcttttcgga
     5281 tatctttttc agcaatataa tctacattgt gcagggtcgc acggctaaca gtcgtaccgg
     5341 caagttgtac tggtgttaga ttagcagttg gagttacaac accggtacgg ccaactgtcc
     5401 agtcaactga taagagttga gcttcttttt cttcagcagg gaacttgtag gctactgccc
     5461 actttggagc cttaacggta aaaccaagtt cttcttgact tgctaggtcg ttgaccttga
     5521 ttaccactcc atcaatatcg taaggcagat tttcccgttc ctgtcctact tcttggataa
     5581 aattccagat ttcatctatg ttttcagcca agattcgctt aggattgacc acaaaaccta
     5641 gttgctctag gtacttcaaa cccttttctt ggctatcacg agttgaaggg ctggcttctt
     5701 gatagagaaa cgttgcaaga ttacgcttgg caactactgc tgtatccaac tgacgcagag
     5761 ttcctgctgc cgcattacga ggattagcaa attcaggctc tccattttct tggcgcactt
     5821 ggttaacttg gtcaaaggaa gcgcgtggca tgtaacattc cccacgaact gtgatatcta
     5881 gttcttctgg caaagtcaaa gggatgtcct taacacgctt gaggttttct gtgatatttt
     5941 caccaattga accatctcca cgtgttaccc cagcaaccaa aatccccttt tcataagtca
     6001 gcgagataga taagccatcg attttcagct cacaaatata ggtcggatga gccacttcct
     6061 tacgaacacg cgcatcaaaa gcatctagct cctcacgtga aaaagcatcc tgcaaactat
     6121 aaagaggata ctgatgactg tatttttcaa aaccatctaa aaccttgcca ccaacacgat
     6181 gagtcggact gtctgctagc acttgctctg gataagcagt ttctaactcg accaactcac
     6241 ggtaaaggcg gtcatactca ctgtctgaaa ccgagggatt atcgctggta tagtactcag
     6301 tcgcatagcg attgagcaaa gcgactaact cattcattct tttattcata agaccatttt
     6361 accataaaac aagccctcct cacaaacgag aaggacggaa aaaacactta gtttgaaatt
     6421 atttttgaaa ctcaagcaac cttatatcaa tttttcaaaa tgagttcgaa cataaataaa
     6481 cgatatacaa gacaagatga taacaccact tccaattatc aggaaagaag agagatgtac
     6541 acttggcaag actgtcataa atccttttgc aataggcata aatagaatag ctaaggtaaa
     6601 aattgtactc agtactctcc caagaaattc gctctcaacc ttggtttgta cttgagtaaa
     6661 aaagtgaata ttaaaaatcg tcataaacaa ttcacaaact aaatttccag aaaaggaaag
     6721 aaaagttgga agtggtaatc ccatcataaa aactccgaca cctgtcaaag ccagtaaaat
     6781 caaaagatta taaatattag ctttaatttt actagctaga agagccccaa tgatggaacc
     6841 aatagccccc atagttaaaa tacttgcata ggctccttct gatccgtaaa gctgattcga
     6901 aaagggaagt agaaattcaa aagctgcaaa aaagaaatta acgctggaag ctaccagcaa
     6961 aaggaagaaa atttcttgct gatgccagat atagtgtaac ccatccttga tatctacaaa
     7021 aatatctctc ccagtaaaag cctttttctc ttgaactttt gcttcctctt ttggaaggaa
     7081 agccactaga acaaaagcaa tgaaaaaagt cagcgagtct agcagtagcg tcatatggag
     7141 acttgcaaac tgtaaaacaa ggaaggaaag aacaggagag ctaacaccta caacctgcaa
     7201 aaccagctct aggcgagaat tatagatcac aatctcatct ttctccacca cttcagttat
     7261 gatagcttta ttggctgtgc gagaaaaggc aaaagcaata gcctgcacaa tgttagcaac
     7321 aatcaaagcg ccaatcatcc agctatcatt ccttatgaaa gaaatagcca gacaaagaat
     7381 cccacaaaca agatctgccg tcattaaaat cttacgacga gaaaaacggt ctgaaataac
     7441 tccgccaaag ggattgacga gaatagatgt gacgagctca gaaatctgat acattcctaa
     7501 aactgtctgt cctatagtcc ccatagaagc caaccagaca ctatttccat aatcatagag
     7561 catatttccc attttattga tagccccacg gctaatcaac tgcactgcat agcgattcat
     7621 attaaagctc ctctcaaatt ttgaaactat tgtatcaaaa ccgaaaggag ctttttattt
     7681 tttcccttat ttgggaaaat taacttttga caaatttttc gtagtgttcc tgataatagg
     7741 ctacttgctc tggaagacct aacacatcaa aaatatgtat ggcctcttgc atctgcttac
     7801 agccttcttt acactgtcct ttttgatata aggcaaaacc ttttaaataa tggaaaacat
     7861 tacgctcata aagcttaata cctttgtcaa taatcttctc tgtataagcc tcaaaatagt
     7921 tggcattata aaaagaagaa tgctctaaac aatgctggta acaattgagg gccaaaatca
     7981 aaactaatct cttatggcga ctaatctctt ggtaaaattc ctccctctcc ataacttctc
     8041 taccaatccg agtgacatag tctacatcgt agaaactata gaggttaccg aaaagaatca
     8101 actcatacat ggtccattct tctgttttga agagataatc tgctacctta tccaaatcat
     8161 cctgcttcat atcataactc gcatctcttt gacaaatcag accttgtagc aaaatccagt
     8221 tcagctcaaa ataaagggga gtcgtcgaac tcttagactt ttcaagttgt tctctttgaa
     8281 gcttttgaaa acctgcaata tcgtttgaat agtaaagtgg gataatctgt gccatcatag
     8341 acacatgttc atgattgtga aaattccttg ccttatccat gaaattttcg attgttacat
     8401 gaatgttatc caaaatctca aagaaacggg agactgccag gtcagactcc ccaagctcaa
     8461 agcgagataa ctgagaggta gagcaggatt cgcctgctgc ttcctttaaa gaataatttc
     8521 cacttgttcg aaattcacga aatacttttc caagatgttc catctttaca cctgatctga
     8581 taattcttcc cactcaagca tagcttcttc ctgacgatgg ctgattttgt ccagctcagc
     8641 ctgtaattcc atgagtttgt cggcatcgtt tgtttccaac atttgttcag aaatggcttg
     8701 gctttgactt tctagctctt caatttcagc ttctagactt tcgatttgtc gcatgagttt
     8761 gcgaacttct ttttgacttt ctttctgggc ctgatagtca ttgactggac ttgcttcctt
     8821 tgcttgattg ctagttgaag cttcctcagt ctgactcatt tctgctgttg ctttcttctc
     8881 aacatagtag tcgtaatctc caaggtagag agttgaacca ttctcagaca attccaaaac
     8941 atgagttgcc acacgattga taaagtaacg atcatgactg acaaaaagca aggtaccatc
     9001 aaagtcaatc aaagcatttt ccaacacttc cttgctatca atatccaagt ggttggtcgg
     9061 ctcgtcaagg atcaggaagt tgttgttttc catagacaat ttagctaaaa gcaaacgagc
     9121 tttttcgcca ccagatagca tgccgactga ttttttaaca tcatctcctg agaaaaggaa
     9181 ggctccaaga cggttgcgga tttcaacttc tggtgtcagt ttgaaatcat tccagagttc
     9241 atccagcacc gtattacttg gtgtcagctt gctttgggtt tggtcatagt aaccaacctc
     9301 aacattagcg ccaaagcgct tttcaccttt gataaaagga atctggtcca caatagactt
     9361 gataaaggtt gacttgccga taccatttgg accaacgata gcgacagcat tcatcttacg
     9421 aagatctagg ttgataggtt gtgacaagac ttccccgtca taaccaatag cagtattttc
     9481 aacagtcaaa acaacattgc ccgacgtttt ttcagactgg aaggtcatgt tggctgcttt
     9541 cttgccagct tcaggcttgt ccaaacgctc cattttttct agttgtttac ggcgagattg
     9601 agcacgttta gtcgttgaag cacgaactag attgcgattg acaaagtctt ccagagcagc
     9661 gatttccttc tgttgctttt catagttttt tgcctcagta actagctttt gctccttcaa
     9721 ttcgacaaaa cgagagtaat tccccacata gcgatccaag gaatgcttgg tcaaatctag
     9781 cgtaattgtc gcaaccttgt ccaagaaata acggtcgtgg ctgacgataa tgagggcacc
     9841 gctatagttt accaagtaat tctctagcca ggcgatggtt tcaatatcca agtggttagt
     9901 tggctcgtcc aagaccaaga gattgggctt ttcaaggagc attttggcaa gtgccaaacg
     9961 agtattttga ccaccagaaa gctcagcaat tttcatctgc cacatagact cgtcaaactt
    10021 gaatccattc aaaatcgctc gaatatcagc ttcataggta aagccacctg cttggcgaaa
    10081 attctcagat aagcggtcat aatctgacat cagtttatcc aaatcctcac cagacttttc
    10141 acccatctcc agctccatct gacgcagttg tctctccgtc cgacgcaaat tattaaagac
    10201 atgaagcatt tcatcgtaga tggtattttc agactcaaaa cggctatctt gggctaggta
    10261 agacagagaa atatcttttt tcttattgat ttctccgcta gttggctcct cttctccaac
    10321 taaaatcttc aaaagagtag acttacctgc accatttttc ccaacaaggg caatccgatc
    10381 tcgttcatca acctgcaggt tgatattatc gaaaagaacc tctcctgcaa aagaacgttc
    10441 aattttatta gcttgtaaaa taatcataag agtagtatag catgtttccc taaggcattc
    10501 a
//
LOCUS       DAVLVP010000063        10388 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.063, whole genome shotgun sequence.
ACCESSION   DAVLVP010000063 DAVLVP010000000
VERSION     DAVLVP010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10388)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10388)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10388
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.063"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..942)
                     /locus_tag="VYN69_001491"
     CDS             complement(<1..942)
                     /locus_tag="VYN69_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element ISSpn8 family transposase"
                     /protein_id="HEV1533744.1"
                     /translation="MQEHYTPKGKHLTIDNRRLIERWKNENKSNREIAGLLGKAPQTI
                     HNEVKRGTTLQQVRKGLYKKVYSADYAQTVYQFNRKRSVKKLILTKEIREKILHYHKQ
                     KFSPEMMVNKKQVKVGISTIYYWFHNGHLGLTKADMLYPRKRKGVKKQASPNFKPAGK
                     SIEERPDVINLRLENGHYEIDTVLLTKIKNYCLLVLTDRRSRHQIIRLIPNKTAESVN
                     QALTLLLGEHRILSITADNGSEFKRLSEVFPEEHIYYAHAYSSWERGSNENHNRLIRR
                     WLPKGTKKTTPKEVAFIENWINNYPKKCLDYKSPSEFL"
     gene            1546..2589
                     /locus_tag="VYN69_001492"
     CDS             1546..2589
                     /locus_tag="VYN69_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00341 family protein"
                     /protein_id="HEV1533745.1"
                     /translation="MTNNKLYSLIEFRNKIYEELELSRSDLAILLCAMLIASIGLNMD
                     STPVIIGAMLISPLMTPILGVGLSLAIFDFKLLRKSFKILAIQILASLIASTLYFYLS
                     PISYASSEIVARTSPTIWDVLIAFVGGIAGIIGARKKETNNIVPGVAIATALMPPLCT
                     VGYAIASANLKFIIGSSYLFLINCSFIVIATYIGVRLMMVKKHYFKDNEEDSKMRRIL
                     LLVAVLLMIPSFISATTLVRETLKKESLKKFISEQFQGYNILKKTYSKKTHTLKLTIS
                     GNYLTEEELDMISSKRGDYGLSDVSVQVSQLSDSEQLSKEELVEHFFQYIKDKEAKEK
                     EKANKFDTESEEQ"
     gene            2756..4843
                     /gene="topA"
                     /locus_tag="VYN69_001493"
     CDS             2756..4843
                     /gene="topA"
                     /locus_tag="VYN69_001493"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEV1533746.1"
                     /translation="MATATKKKKSTVKKNLVIVESPAKAKTIEKYLGRNYKVLASVGH
                     IRDLKKSSMSVDIENNYEPQYINIRGKGPLINDLKKEAKKANKVFLASDPDREGEAIS
                     WHLAHILNLDENDANRVVFNEITKDAVKNAFKEPRKIDMDLVDAQQARRILDRLVGYS
                     ISPILWKKVKKGLSAGRVQSIALKLIIDRENEINAFQPEEYWTVDAVFKKGTKQFHAS
                     FYGVDGKKMKLTSNDEVKEVLSRLTSKDFSVDQVDKKERKRNAPLPYTTSSMQMDAAN
                     KINFRTRKTMMVAQQLYEGINIGSGVQGLITYMRTDSTRISPVAQNEAASFITDRFGS
                     KYSKHGSKVKNASGAQDAHEAIRPSSVFNTPESIAKYLDKDQLKLYTLIWNRFVASQM
                     TAAVFDTMAVKLSQKGVQFAANGSQVKFDGYLAIYNDSDKNKMLPDMVVGDVVKQVNS
                     KPEQHFTQPPARYSEATLIKTLEENGVGRPSTYAPTIETIQKRYYVRLAAKRFEPTEL
                     GEIVNKLIVEYFPDIVNVTFTAEMEGKLDDVEVGKEQWRRVIDAFYKPFSKEVAKAEE
                     EMEKIQIKDEPAGFDCEVCGSPMVIKLGRFGKFYACSNFPDCHHTQAIVKEIGVECPS
                     CHQGQIIERKTKRNRLFYGCNRYPECEFTSWDKPVGRDCPKCGNFLMEKKVRGGGKQV
                     VCSKGDYEEEKIK"
     gene            4949..5308
                     /locus_tag="VYN69_001494"
     CDS             4949..5308
                     /locus_tag="VYN69_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004258004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HEV1533747.1"
                     /translation="MRLIYLIIGFLSLALAIVGVVLPLLPTTPFLLLSIACFSRSSKR
                     FEDWLYHTKLYQTYVADFRETKSITRERKKKIIVSIYVLMGISIYFAPLLPVKIGLGA
                     LTIFITYYLFKVIPDKE"
     gene            5401..6033
                     /locus_tag="VYN69_001495"
     CDS             5401..6033
                     /locus_tag="VYN69_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000638771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEV1533748.1"
                     /translation="MIYEFCAENVTLLEKAMQAGARRIELCDNLAVGGTTPSYGVTKA
                     AVELAANYDTTIMTMIRPRGGDFVYNDLEIAIMLEDIRLTAQAGSQGVVFGTLTADKK
                     LDKPNLEKLIAASKGMEIVFHMAFDELSDEDQLEAIDWLSQAGVTRILTRAGVSGDSL
                     EKRFVHYHRILEYAKGKIEILPGGGIDLENRQTFIDQVGVTQLHGTKVVF"
     gene            6077..6733
                     /locus_tag="VYN69_001496"
     CDS             6077..6733
                     /locus_tag="VYN69_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000461593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEV1533749.1"
                     /translation="MFEIFKSYQFNQEKAHDYGFIENSEVWTYSCQILQGDFVMTVSI
                     TADNVNFQVFDQETGDLYPHVHMESMRGSFVGNVREACLEILYQIRKACFDVQDFICH
                     QTKRIMTQVQEKYGNQLEYLWEKSPDTAVLRHEGNQKWYAVLMKISWNKLEKGREGQV
                     EAVNLKHDQVANLLSQKGIYPAFHMSKRYWISVSLDDTLSDEEVLELIEKSWNLTSKK
                     "
     gene            6752..7846
                     /locus_tag="VYN69_001497"
     CDS             6752..7846
                     /locus_tag="VYN69_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HEV1533750.1"
                     /translation="MNFQLAKYSLLKRFLENIGFTTLEEYGAIFKYLIENVKTDRQII
                     YSPHCHDDLGMAVANSLAAVKNGAGRVEGTINGIRERAENAALEEIAVALNIRQDYYQ
                     VETSIVLNETINTSEMVSRFSGIPVPKNKAVVGGNTFSHESGIHQDGVLKNPLTYEII
                     TPELVGVKILLGKLSGRHAFVEKLRELALDFTEEDIKPLFAKFKALVDKKQEITDADI
                     LALVAGTMVENPEGFHFDDLQLQTHADNDIEALVSLANMDGEKVEFNATGQGSVEAIF
                     NAIDKFFNQSVRLVSYTIDAVTDGIDTQDRVLVTVENRDTETIFNAAGLDFDVLKASA
                     IVYINANTFVQKENAGEMGRSVSYHDMPSV"
     gene            7858..8895
                     /gene="leuB"
                     /locus_tag="VYN69_001498"
                     /pseudo
     CDS             7858..8895
                     /gene="leuB"
                     /locus_tag="VYN69_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399454.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
     gene            8892..9161
                     /locus_tag="VYN69_001499"
     CDS             8892..9161
                     /locus_tag="VYN69_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEV1533751.1"
                     /translation="MKLDEKITLVLLIWNVIIFLIYGIDKFKARRRAWRIPEKILLIL
                     ALTCGGFGAWLAGITFHHKTRKWYFKTVWFLGMVTTLVALYYIWR"
     gene            9165..9524
                     /gene="leuD"
                     /locus_tag="VYN69_001500"
     CDS             9165..9524
                     /gene="leuD"
                     /locus_tag="VYN69_001500"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HEV1533752.1"
                     /translation="MAGSSREHAAWALADYGFKVVIAGSFGDIHYNNELNNGMLPIVQ
                     PREVREKLAQLQPTDQVTVDLEQQKIISPVEEFTFEIDSEWKHKLLNTLDDIGITLQY
                     EELIAAYEKQRLAYWQD"
     gene            9686..10308
                     /locus_tag="VYN69_001501"
                     /pseudo
     CDS             9686..10308
                     /locus_tag="VYN69_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002276310.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="translation factor (SUA5)"
BASE COUNT         3156 a         1870 c         2253 g         3109 t
ORIGIN      
        1 aagaaattca cttggcgact tgtagtccaa gcatttttta gggtagttgt taatccaatt
       61 ttcgataaaa gctacttctt tcggagtcgt tttcttggtt cctttaggta accatctccg
      121 aattaatcga ttatgatttt catttgaacc tctctcccat gaagagtaag catgtgcgta
      181 gtagatatgt tcctcaggaa atacctcaga caatcgtttg aactccgaac cattatctgc
      241 agtaatggac agaatacgat gctcccctaa tagtaacgta agcgcctgat tgacagattc
      301 agcagtttta tttggaatta accttataat ttggtgtctg ctccgccggt cggttaagac
      361 taacaggcaa taatttttta tcttagtcag taggacggta tcaatttcat aatgaccatt
      421 ttccaagcga agattaataa cgtcaggacg ttcttcgata gatttacctg ccggcttaaa
      481 gttcggacta gcttgcttct tgacaccttt cctttttctg ggataaagca tgtcggcctt
      541 cgtcaatcct aaatgaccat tatgaaacca gtagtagatg gttgaaatac caactttcac
      601 ttgcttcttg ttaaccatca tttcaggcga aaatttttgc ttatgatagt gtaagatctt
      661 ctctctgatt tcctttgtta aaattaactt tttcaccgac cgttttcgat tgaattggta
      721 aacagtttgt gcgtaatcgg cagaatagac ctttttgtat agcccttttc tcacttgttg
      781 taaagttgta cctcttttga cttcattatg aatcgtttga ggcgcctttc ctaacaagcc
      841 tgcaatttca cgattggact tattttcatt cttccaccgc tcaatcaagc gacggttatc
      901 tattgtcaaa tgtttccctt ttggggtata atgttcttgc atctctgtgc ctttccttgt
      961 gtttgtggtg aacaacaagt atagcacaga ggtgttttct tataccttaa atcacatttc
     1021 atttgacaac aggaaccctt gaacctgttt tttcagctaa accaaggcta tgattagcct
     1081 tggccaccta gcctaacagt ttcaaacctg ttatcaaatg aaatcatcaa cttactcaaa
     1141 catagtgaaa catctaattc ttgactaagg taaggtggct aacttcatta tagaactttc
     1201 aaagtagaga ttacaaggct tgtttaagaa agaattcaaa gaccttgaca aataaaaata
     1261 aaatggttat tataaaaaat ggtctgaaat agatgatgat acttttcgaa aatcttttta
     1321 cgtcagctca gctttgcctt gctgtgtttt gagcaagcta cggttagctt ccgagtttga
     1381 ttttcattta ctagaaatga aactgatgag agatatcagt agacatttga gtcaggatat
     1441 tatggaaaat gataaaaaga gctcgtgaga ttggcatatc agactactaa agtattgagt
     1501 ttgttaggat tttagcgact agttagctgg gaaaggaaga tatttgtgac aaataataaa
     1561 ctgtattcgt tgatagaatt tagaaataaa atatatgaag aattagaact ttccagaagt
     1621 gatttagcga ttttactatg tgccatgctt atcgcctcta tcggattaaa tatggattcg
     1681 actcccgtga ttattggagc catgttaatc tctcctttga tgacacctat tctgggagtg
     1741 gggctctctc tagctatatt tgattttaaa ttgttaagaa aatcttttaa aatattagct
     1801 attcaaattc ttgccagttt aatagcttca acactttatt tttatctttc tcccatttcg
     1861 tatgctagtt cggaaattgt tgctagaacc tctccgacta tttgggatgt tctcattgct
     1921 tttgtaggag ggatagcagg tattattggt gctaggaaaa aagagaccaa taatattgtt
     1981 cctggtgttg ctattgcaac cgccttgatg cctcctcttt gtacagtagg ttatgctatt
     2041 gcttctgcta atctaaaatt tatcataggc tcctcttacc tattcctcat caattgtagc
     2101 tttattgtca ttgcgactta tataggtgtt aggttgatga tggttaagaa acattatttt
     2161 aaagataatg aagaagactc taaaatgcgt aggattttgc ttctagttgc tgttttgctg
     2221 atgattccga gtttcatctc tgcaacgact ttagtgagag aaacgttgaa aaaagagtcc
     2281 cttaagaaat ttatatcaga gcagtttcag gggtataata ttttgaaaaa aacctattct
     2341 aaaaagactc ataccctaaa gctaaccatt tcaggaaatt atttgacaga agaagaactc
     2401 gatatgattt caagtaagag aggtgactat ggtttaagtg atgtttctgt tcaagtttca
     2461 caattgtctg attcagaaca acttagtaag gaagagctgg tggagcattt cttccagtat
     2521 atcaaggata aggaagcaaa agaaaaggag aaagccaata aatttgatac agagtctgag
     2581 gagcaataat ttcttgagaa tagctggttt ctctcgtgag tcttctatgt atatcaaagg
     2641 aagactgagg ttttaagtat aaaacttttc ttctattata gtagctaagt cttgacaggg
     2701 ttcaaaaaat ggtttacact ttataaagtt tattactttg aaaaggtgtg atactgtggc
     2761 tacggcaaca aaaaagaaaa aatcaacagt taaaaaaaat ctagtcatcg tggagtcgcc
     2821 tgctaaggcc aagacgattg aaaaatatct aggcagaaac tacaaggttt tagccagtgt
     2881 cgggcatatc cgtgatttga agaaatccag tatgtccgtc gatattgaaa ataattatga
     2941 accgcaatat attaatatcc gaggaaaagg ccctcttatc aatgacttga aaaaagaagc
     3001 taaaaaagct aataaagttt ttctcgcgag tgacccggac cgtgaaggag aagcgatttc
     3061 ttggcatttg gcccatattc tcaacttgga tgaaaatgat gccaaccgtg tggtcttcaa
     3121 tgaaatcacc aaggatgcag tcaaaaatgc ttttaaagaa cctcgtaaga tcgatatgga
     3181 cttggtcgat gcccaacaag ctcgtcggat cttggaccgc ttggtagggt attcgatttc
     3241 gcctattttg tggaagaagg tcaagaaggg cttgtcagca ggtcgcgttc agtccattgc
     3301 ccttaaactc atcattgacc gtgaaaatga aatcaatgcc ttccagccag aagaatactg
     3361 gacagttgat gctgtcttta aaaagggaac caaacaattt catgcttcct tctatggagt
     3421 agatggtaaa aagatgaaac tgaccagcaa tgacgaggtc aaggaagtct tgtctcgtct
     3481 gactagtaaa gacttttcag tagatcaggt ggataagaaa gagcgcaagc gcaatgctcc
     3541 tttaccctat accacttcat ctatgcagat ggatgctgcc aataaaatca atttccgtac
     3601 tcgaaaaacc atgatggttg cccaacagct ctatgaagga attaatattg gttctggtgt
     3661 tcaaggtttg attacctata tgcgtaccga ttcgactcgt atcagtcctg tagcgcaaaa
     3721 tgaggcggca agcttcatta cggatcgttt tggtagtaag tattctaagc acggtagcaa
     3781 ggtcaaaaac gcgtcaggtg ctcaggatgc ccatgaggct attcgtccgt caagtgtctt
     3841 taatacacca gaaagcatcg ctaagtatct ggacaaggat cagctcaagc tatataccct
     3901 tatctggaat cgttttgtgg ctagccagat gacagcggcc gtttttgata ccatggctgt
     3961 taaattgtct caaaaagggg ttcaatttgc tgccaatggt agtcaggtta agtttgatgg
     4021 ttatcttgcc atttataatg attctgacaa gaataagatg ttaccggaca tggttgttgg
     4081 agatgtggtc aaacaggtca atagcaaacc agagcaacat ttcacccaac cgcctgcccg
     4141 ttattctgaa gcaacactga ttaaaacctt agaggaaaat ggggttggac gtccatcaac
     4201 ctacgcgcca accattgaaa ccattcagaa acgttattat gttcgcctgg cagccaaacg
     4261 ttttgaaccg acagagttgg gagaaattgt caataagctc atcgttgaat atttcccaga
     4321 tatcgtaaac gtgaccttca cagctgaaat ggaaggtaaa ctggatgatg tcgaagttgg
     4381 aaaagagcag tggcgacggg tcattgatgc cttttacaaa ccattctcta aagaagttgc
     4441 caaggctgaa gaagaaatgg aaaaaatcca gattaaggat gaaccagctg gatttgactg
     4501 tgaagtgtgt ggtagtccaa tggtcattaa acttggtcgt tttggtaaat tctacgcttg
     4561 tagcaatttc ccagattgcc atcataccca agcaatcgtg aaagagattg gtgttgagtg
     4621 tccaagctgt catcagggac aaattattga gcgaaaaacc aagcgtaatc gcctattcta
     4681 tggttgcaat cgctatccag aatgtgaatt tacctcttgg gacaagcctg ttggtcgtga
     4741 ctgtccaaaa tgtggcaact tcctcatgga gaaaaaagtc cgtggtggtg gcaagcaggt
     4801 tgtttgtagc aaaggcgact acgaggaaga aaagattaaa taagaggagg ggcccgaaag
     4861 taaatttcag gctccttttg gttagaactt gacaaaatac atcattttat gcgaaactag
     4921 aagaagatta ttttaactag gagaagttat gcgtcttatc tatctgataa ttggtttttt
     4981 atcactggcc ttggctattg ttggggttgt tttacccttg ttgccgacaa cgcctttcct
     5041 tttgttgtct attgcttgtt tctccagaag ttccaagcgt ttcgaagact ggctttatca
     5101 taccaagctc tatcaaacat atgtagctga ttttcgcgag accaagtcta ttacgcgtga
     5161 acgaaagaaa aaaatcatcg tctctatcta cgtcttgatg ggaatttcta tttattttgc
     5221 acctctttta ccagtcaaaa tcggtctggg tgctttgacc atctttatca cttattatct
     5281 cttcaaggtc attccagaca aagaatagtt aaaacagtag ttatttgcct tgataaaatt
     5341 gaaagcatat tcataacaat atgatataat aaatttgaag taatattcaa ggagaatcaa
     5401 atgatttacg aattttgtgc tgaaaatgtg actttacttg aaaaagcgat gcaggctgga
     5461 gctcgtcgga ttgaactctg tgataatcta gcagttggtg ggacaacacc cagctatgga
     5521 gtgactaagg cagcggttga actggcagct aactacgata caaccatcat gaccatgatt
     5581 cggccacgtg gtggtgactt tgtctataat gacctagaaa ttgctatcat gctagaagac
     5641 attcgtttga ctgctcaggc tggaagtcaa ggggttgtat ttggaacttt aactgctgat
     5701 aaaaagttgg ataagcctaa tctggaaaag ttaattgctg catcaaaagg aatggaaatt
     5761 gtctttcaca tggcctttga tgaactaagt gatgaagatc aactggaagc tattgactgg
     5821 ctcagtcaag ccggtgtcac tcgtatccta actcgtgctg gtgtgtctgg cgactcctta
     5881 gaaaaacgtt ttgttcacta tcacagaatt ttggagtacg ctaaaggtaa aattgaaatt
     5941 ctaccaggtg gggggattga ccttgaaaac cgtcaaacct ttatcgacca ggtgggggta
     6001 acacaattgc atggtactaa ggttgttttt taaaaaatag aaaggaactg ctagccttgg
     6061 gtagcagttt tcacttatgt ttgaaatttt taaatcctat caatttaatc aagaaaaggc
     6121 tcatgattat ggttttatag aaaatagcga agtctggaca tatagttgcc agattttgca
     6181 aggtgacttt gtcatgactg tgtccatcac tgctgataat gtgaactttc aagtctttga
     6241 ccaagagact ggtgacctct atcctcacgt tcatatggaa agcatgaggg gaagttttgt
     6301 cggaaatgtc cgtgaggctt gtctggagat tctttaccag attcggaagg cttgttttga
     6361 tgtgcaagat tttatctgtc atcagactaa gcgtatcatg actcaagttc aggaaaagta
     6421 tggaaaccag ttggagtatc tgtgggaaaa atcgcctgat acagctgtat tgcgccatga
     6481 aggcaatcaa aagtggtatg ccgtcttgat gaaaatctct tggaataagc tggaaaaggg
     6541 cagagaagga caagtggaag cagtcaacct caagcatgac caagtagcta atttgctttc
     6601 acaaaagggg atttatccag ccttccatat gagcaagcgc tactggatta gtgtgtccct
     6661 tgatgatact ttatcagatg aagaagtact ggaattgata gaaaaaagtt ggaacttaac
     6721 ctctaaaaaa tgaaatattt taataatttt catgaacttt caattagcta aatattcttt
     6781 actgaagaga tttttagaaa atataggatt taccacacta gaggaatatg gtgccatctt
     6841 caaatacctg attgagaatg tcaagacgga tcgtcagatc atctattcgc ctcactgtca
     6901 tgatgacctc ggaatggcag tggcaaatag ccttgctgct gtcaagaatg gtgcaggacg
     6961 tgttgaaggg actatcaatg gtattaggga gcgagctgaa aatgctgctt tggaagaaat
     7021 tgcagtggct ctcaatattc gccaagatta ctaccaagta gaaaccagta ttgtcctaaa
     7081 tgagaccatc aatacgtcag aaatggtttc tcgcttctct ggtattccag ttcctaaaaa
     7141 caaagccgtc gttggtggca ataccttctc ccacgaatct ggtattcacc aagatggagt
     7201 ccttaaaaat cctctcactt atgagatcat cacacctgaa ttggttggtg ttaagattct
     7261 gcttggaaaa ttatctggtc gccatgcttt tgttgagaaa ctgagagaat tggccctaga
     7321 ttttacagaa gaggatatca aaccactctt tgctaagttc aaggcactgg tcgataagaa
     7381 gcaagaaatc acagatgcag atattctagc tttggtagct ggaaccatgg ttgaaaatcc
     7441 agaaggcttc cactttgatg atttacaact tcaaactcat gcagataatg acattgaagc
     7501 gctcgttagc ctagccaata tggatggtga gaaagtcgaa tttaatgcga cagggcaagg
     7561 ttccgttgaa gcaatcttta atgctatcga taagttcttt aaccaatctg ttcgtttggt
     7621 gtcctacact atcgatgcgg taacagatgg aatcgatacc caggatcggg ttttggtcac
     7681 tgttgaaaac agagatacag aaaccatctt taatgcagca gggcttgatt ttgatgtgtt
     7741 gaaggcttct gctattgtct atataaacgc taataccttt gttcaaaaag agaatgcagg
     7801 tgagatggga cgcagtgttt cttaccacga tatgcctagt gtgtaaagga gaaggctatg
     7861 gcaaagaaaa tagtagctct agcaggagac ggaattggcc cagaaatcat ggaggttggt
     7921 ttagaagttc tggaggctct agctgaaaaa acaggttttg actatgagat tgacagacga
     7981 ccgttcggag gtgcagatat tgatgcagca tgacctccct tacctgatga aacccttaag
     8041 gcaagtaggg aagcagatgc tatcctacta gcagctatcg gtagtcctca gtatgatgga
     8101 gcagtggttc gccctgaaca aggcctgatg gctctccgta aggaactcaa tctttacgct
     8161 aatattcgtc ctgtaaaaat ctttgacagt ctcaagcatt tgtcactact caaactggaa
     8221 cgaattgctg gtgtagactt tgtcgtggtg cgtgaattga caggcgggat ttactttgga
     8281 gatcatattc ttgaagagcg caatgcgcgt gatatcaaca actatagcta tgaggaagtg
     8341 gagcggatta ttcgcaaagc ctttgaaatt gcaagaaatc gcagaaaaat cgttactagt
     8401 atcgataagc aaaatgttct agcgacctca aaactctggc ggaaagtagc tgaggaagtc
     8461 gcacaggatt tcccagatgt aaccttggaa catcagctgg tagactcagc tgctatgctt
     8521 atgattacca atcctgctaa gtttgatgtt attgtaacgg agaatctttt tggagatatt
     8581 ttatctgatg aatcaagcgt cttatctggt acacttgggg ttatgccatc agccagtcat
     8641 tctgaaaatg gaccaagtct ctatgaacct attcacggtt cagtacctga tattgcaggt
     8701 caaggaattg ccaatcctat ttccatgatt ttatcagttg ccatgatgtt aagagatagt
     8761 ttcggaggtt atgaggatgc agagcgtatc aaacgtgctg ttgagacaag tctggcggca
     8821 ggaattttaa cgagagatat aggaggtcag gcttcaacaa aggaaatgac ggaagctatt
     8881 attgcaaggt tatgaagtta gacgaaaaaa ttactctagt ccttttgatt tggaatgtca
     8941 tcattttctt gatttatggc attgacaaat tcaaggcaag aagaagagct tggcgcatcc
     9001 cagagaaaat cttactcatt ttagccctta cttgtggtgg ttttggggcc tggttagctg
     9061 gaatcacttt tcaccataag actcgaaaat ggtactttaa aacagtttgg tttcttggga
     9121 tggtgactac actagtagcc ttatattata tttggaggta atagatggca gggtcttcga
     9181 gggaacacgc tgcttgggct ctagcggact atggttttaa ggtcgtgatt gcaggatctt
     9241 tcggtgacat tcattacaat aatgaactca ataatggcat gttgccaatc gttcagccta
     9301 gagaggttag agagaaacta gcccagctac aaccaaccga tcaggtaact gtggacttgg
     9361 aacaacaaaa aatcatctca ccagttgaag aattcacctt cgagatagat agcgagtgga
     9421 aacataaact cctaaatact ttggatgata tcggtattac cttgcagtat gaagagttga
     9481 ttgctgctta tgaaaaacaa cgactagcct actggcagga ttagaaaaaa tagaaaagga
     9541 aatatagtaa actgaaataa gatgtaaaca aatgaattgg agcttaacat caatttccag
     9601 caatttttta gaaactacag tggactattc tggattcaac acattataaa attatgacaa
     9661 aacacattca caagaaggct acgacatttt aaaaggtgag ggcggatgta tcgtttgccc
     9721 tactaaagtt ggttacatta tcatgaccag caacaaggca ggacttgagc gtaagttcgc
     9781 agccaaagaa cgtaagcgta acaaaccagg tgttgttctc tgcggtagca tggatgaact
     9841 ttgcgcttta gcgcaactca acccagaaat tgaagcattc tactaaaaac attgggatga
     9901 agatattctt cttggttgta tccttccttg gaaaccagaa gcctttgaaa aactcaaagc
     9961 atacggggat ggccgtgaag aacttatgac tgatgtacgt ggtactagct gttttgttat
    10021 caagtttgga aaagcaggtg aacaattggc tgccaagctt tgggaagaag gtaaaatggt
    10081 ctacgcctca tctgcttcaa tgacaaaacg actgaaactc gctatgagca aggtgtaatg
    10141 gtgtctatgg tcgataagga cggcaaactc atcccagaac aaggaggagc acgttcaact
    10201 tcaccagcac cagttgtgat ccgtaaaggg cttgacattg ataaaatcat gatgcacctg
    10261 tcagatactt ttaactcatg ggactaccgt caggttgagt attattaaga taaaaaaaga
    10321 agtctagtgt tatgagatat taaagctcct aacactggac tttttgttta gaatttcttt
    10381 tctttttc
//
LOCUS       DAVLVP010000064        10221 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.064, whole genome shotgun sequence.
ACCESSION   DAVLVP010000064 DAVLVP010000000
VERSION     DAVLVP010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 10221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10221
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.064"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..190
                     /locus_tag="VYN69_001502"
     CDS             <1..190
                     /locus_tag="VYN69_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000242196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEV1533753.1"
                     /translation="MIASDKEVEVSLLFFIINNSSSSQTNILAQLMDFFTGNGHLEDL
                     SKISEPEKLYAYIAEATA"
     gene            228..530
                     /locus_tag="VYN69_001503"
     CDS             228..530
                     /locus_tag="VYN69_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1533754.1"
                     /translation="MIKILAACGAGVNSSHQIKSALEEELSNRGYDVHCDAVMVKDVN
                     EDLMKGYDIFTPIAATDLGFEPGIPVIEAGPILFCIPAMSVPVFDNIILPAKQNMV"
     gene            538..1357
                     /locus_tag="VYN69_001504"
                     /pseudo
     CDS             538..1357
                     /locus_tag="VYN69_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
     gene            1394..1870
                     /locus_tag="VYN69_001505"
     CDS             1394..1870
                     /locus_tag="VYN69_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001146193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase"
                     /protein_id="HEV1533755.1"
                     /translation="MPYFIGGHPGFNCPLLDDEVYEDYYLESEKEETCSVPRPFPETG
                     MLDFQDRSPWLEGQKEIDLSYDLFSKDAVTLDELQSRTIALRSLKHDKGLKVHFAEFP
                     NLIIWSTLNKGPFITFEPWSGLSTFLEEGDHLEDKKNVCLLEANQVEELGFEIEVL"
     gene            2027..2452
                     /gene="lacA"
                     /locus_tag="VYN69_001506"
     CDS             2027..2452
                     /gene="lacA"
                     /locus_tag="VYN69_001506"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEV1533756.1"
                     /translation="MSIVIGADAAGLRLKEVVKDFLEKENFHLVDVTAEGQDFVDVTL
                     AVAAEVNKEEQNLGIVIDAYGAGPFMVATKIKGMVAAEVSDERSAYMTRGHNNSRMIT
                     MGAQLVGDELAKNIAKGFVNGKYDGGRHQIRVDMLNKMG"
     gene            2483..2998
                     /gene="lacB"
                     /locus_tag="VYN69_001507"
     CDS             2483..2998
                     /gene="lacB"
                     /locus_tag="VYN69_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002994294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEV1533757.1"
                     /translation="MRIAIGCDHIVTDEKMAVSEFLKSKGYEVIDFGTYDHTRTHYPI
                     FGKKVGEAVTSGQADLGVCICGTGVGINNAVNKVPGVRSALVRDMTTALYAKEQLNAN
                     VIGFGGKITGELLMCDIIEAFIHAEYKPSEENKKLIAKIEHLESHNAQQTDANFFTEF
                     LEKWDRGEYHD"
     gene            3009..3938
                     /locus_tag="VYN69_001508"
     CDS             3009..3938
                     /locus_tag="VYN69_001508"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEV1533758.1"
                     /translation="MILTVTMNPSIDISYPLDELKIDTVNRVVDVTKTAGGKGLNVTR
                     VLSEFGDSVLATGLVGGKLGEFLVEHIDNQVKKDFFSIQGETRNCIAILHGDNQTEVL
                     EKGPEVLEQEGQDFLEHFKKLLESVEVVAISGSLPAGLQVDYYASLVELANQAGKPVV
                     LDCSGAALQAVLESPHKPTVIKPNNEELSQLLGREVSEDLEELKEVLQEPLFAGIEWI
                     IVSLGANGTFTKHGDTFYKVDIPRIQVVNPVGSGDSTVAGISSGLLHKESDAELLIKA
                     NVLGMLNAQEKMTGHVNMANYQALYDQLIVKEV"
     gene            3940..4920
                     /gene="lacD"
                     /locus_tag="VYN69_001509"
     CDS             3940..4920
                     /gene="lacD"
                     /locus_tag="VYN69_001509"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001229146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEV1533759.1"
                     /translation="MALTEQKRVRLEKLSDENGIISALAFDQRGALKRLMAQHQTEEP
                     TVAQMEELKVLVADELTKYASSMLLDPEYGLPATKALDEKAGLLLAYEKTGYDTTSTK
                     RLPDCLDVWSAKRIKEEGADAVKFLLYYDVDSSDELNQEKQAYIERIGSECVAEDIPF
                     FLEILAYDEKIADAGSVEYAKVKPHKVIGAMKVFSDPRFNIDVLKVEVPVNIKYVEGF
                     AEGEVVYTREEAAAFFKAQDEATNLPYIYLSAGVSAKLFQDTLVFAHESGANFNGVLC
                     GRATWAGSVEAYIKDGEAAAREWLRTTGFENIDELNKVLQTTATSWKERV"
     gene            5154..5990
                     /locus_tag="VYN69_001510"
     CDS             5154..5990
                     /locus_tag="VYN69_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000285719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEV1533760.1"
                     /translation="MYRILNPMNHNVSLVRNDKGEEVIVIGKGIAFGKRKGDLIAENQ
                     VEKIFRMKTEESRENFMALLKDVPLDFITVTYEIIDKLSKKYHYPIQEYLYVTLTDHI
                     YCSYQALTQGRYKDSNLPDISAKYPVAFQIANEAFEIYRQKLADHFPEDEIIRIAYHF
                     INAEGENEVELVESIDKRKEILRNVEEVLKGYAIQRTKKNNHFYDRFMIHLNYFLDYL
                     DRSRDDNQSLLDMEDHIKQSYPKAFEIGSKIYDVITQHTGLDLYKSERVYLVLHIQRL
                     LS"
     gene            6026..6343
                     /locus_tag="VYN69_001511"
     CDS             6026..6343
                     /locus_tag="VYN69_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1533761.1"
                     /translation="MNREEVTLLGFEIVAYAGDARSKLLEALKAAEAGDFEKADALVE
                     EAGSCIAEAHHAQTSLLTKEASGEDLAYSVTMMHGQDHLMTTILLKDLMHHLIELYKR
                     GVK"
     gene            6343..8034
                     /locus_tag="VYN69_001512"
     CDS             6343..8034
                     /locus_tag="VYN69_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001037357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEV1533762.1"
                     /translation="MNKLIAFIEKGKPFFDKLSRNIYLRAIRDGFIAGMPVILFSSIF
                     ILIAFVPNSWGFKWSDEVVAFLMKPYSYSMGILALLVAGTTAKSLTDSVNRSMEKTNQ
                     INYMSTLLAAIVGLLMLAADPIENGLATGFLGTKGLLSAFLAAFVTVAIYKVCVKNNV
                     TIRMPDEVPPNISQVFKDVIPFTLSVVSLYALDLLARHFVGSSVAESIGKFFAPLFSA
                     ADGYLGITIIFGAFAFFWFVGIHGPSIVEPAIAAITYANAEVNLNLLQQGMHADKILT
                     SGTQMFIVTMGGTGATLVVPFMFMWLTKSKRNRAIGRASVVPTFFGVNEPILFGAPLV
                     LNPIFFIPFIFAPIANVWIFKFFIETLGMNSFTANLPWTTPAPLGLVLGTNFQVLSFI
                     LAALLIVVDVVIYYPFLKVYDEQILEEERSGKSNDELKEKVAANFNTAKADAILEKAG
                     VDAAQNTITEETNVLVLCAGGGTSGLLANALNKAAAEYNVPVKAAAGGYGAHREMLPE
                     FDLVILAPQVASNFEDMKAETDKLGIKLAKTEGAQYIKLTRDGKGALAFVQAQFD"
     gene            8243..9649
                     /gene="lacG"
                     /locus_tag="VYN69_001513"
     CDS             8243..9649
                     /gene="lacG"
                     /locus_tag="VYN69_001513"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEV1533763.1"
                     /translation="MTKTLPKDFIFGGATAAYQAEGATHTDGKGPVAWDKYLEDNYWY
                     TAEPASDFYNRYPVDLKLAEEYGVNGIRISIAWSRIFPTGYGQVNAKGVEFYHNLFAE
                     CHKRHVEPFVTLHHFDTPEALHSNGDFLNRENIEHFVDYAAFCFEEFPEVNYWTTFNE
                     IGPIGDGQYLVGKFPPGIQYDLAKVFQSHHNMMVSHACAVKLYKEKGYKGEIGVVHAL
                     PTKYPLDPENPADVRAAELEDIIHNKFILDATYLGRYSAETMEGVNHILSVNGGSLDL
                     REEDFTALEAAKDLNDFLGINYYMSDWMEAFDGETEIIHNGKGEKGSSKYQIKGIGRR
                     VAPDYVSRTDWDWIIYPQGLYDQIMRVKKDYPNYKKIYITENGLGYKDEFVDNTVYDD
                     GRIDYVKQHLEILSDAIADGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQERY
                     PKKSAHWYKKVAETQIID"
     gene            9739..9879
                     /locus_tag="VYN69_001514"
     CDS             9739..9879
                     /locus_tag="VYN69_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3884 family protein"
                     /protein_id="HEV1533764.1"
                     /translation="MNFEKCSQIPCLTSEELKSLGKWYVSTGKEWDTISFDSDFMPFQ
                     QS"
BASE COUNT         3250 a         1840 c         2099 g         3032 t
ORIGIN      
        1 tatgatagct tctgataagg aagtagaagt atccctactc ttctttatca ttaacaattc
       61 aagttcaagt caaacaaata ttctggctca gttgatggac tttttcacag gaaatggaca
      121 tcttgaagac ctatcaaaaa tttccgaacc agaaaaactt tatgcttaca ttgctgaagc
      181 aaccgcttaa tcttgtctat taaaaaataa aatcggagga aatccaaatg attaaaattc
      241 ttgctgcctg cggtgcaggt gttaactcaa gtcaccaaat taaaagtgct ctagaagaag
      301 aactttcaaa ccgtggttac gatgttcact gtgatgcagt tatggtgaaa gatgtaaacg
      361 aagaccttat gaaaggttac gatatcttta caccaatcgc tgcaacagac cttggttttg
      421 aaccaggtat cccagttatc gaagctggcc caatcttatt ctgtatccca gcaatgagcg
      481 ttccagtatt tgacaatatt atacttcctg cgaaacaaaa catggtatag tagttccatg
      541 aatgatgaag caagtaaaca actaactgat gcacgattta aatgtcttgt tggtgttcag
      601 cgcaccactt ttaaagagat gttagctgta ttaaaaactg cttatcaact taaacacaca
      661 aaagggggac gaaaacctaa attaagccta gaagaccttc ttatggccac tcttcaatat
      721 gtgcgagaat accgcactta tgaagaaatt gcggctgatt ttggtatcca cggattttgg
      781 tatccacgaa agaaacttac tccgtcggag ccaatgggtt gaagtaactc ttgttcaaag
      841 tggttttacg atttcaagaa ctcctctcag ttctgaggac acggtaatga ttgatgcgac
      901 ggaagtacaa atcaatcgcc ctaaaaaaag aattagcgaa tcattctggt aaaaaaaatg
      961 ccacgctatg aaggctcaag cgattgtcac aagtcaaggg agaattgttt ctttggatat
     1021 cgctgtgaac tattgtcatg atatgaagtt gttcaaaatg agtcgcagaa atatcggaca
     1081 agctggtaaa atcttggctg acagtggtta tcaagggctc atgaagatat atcctcaagc
     1141 acaaactcca cgtaaatcca gcaaactcaa gccactaaca gttgaagata aagcctataa
     1201 ccatgcgcta tccaaggaga gaagcaaggt tgagaacatc tttgccaaag taaaaacgtt
     1261 taaaatgttt tcaacaacct atcgaaatca tcgtaaacgc ttcggattac gaatgaattt
     1321 gattgctggt attatcaatc atgaactagg attctagttt tgcaggaagt ctaataaaga
     1381 aactagcaag gtcatgcctt actttattgg tggacatcca ggatttaatt gtcctctact
     1441 tgatgatgaa gtctatgaag attattatct agagtctgag aaagaagaga cttgctctgt
     1501 tccacgtcct ttcccagaaa caggtatgtt ggatttccaa gatagaagtc catggctaga
     1561 gggacaaaaa gaaatagatc ttagttatga tcttttcagc aaagatgcag tgactttgga
     1621 tgaattgcaa tctcgaacaa ttgcccttcg ttctcttaaa catgataagg gattgaaagt
     1681 gcattttgca gaatttccaa acctcatcat ctggtcaact ttgaacaagg gacctttcat
     1741 tacctttgaa ccatggtctg gcttgtcaac attccttgaa gaaggagatc atttagaaga
     1801 taagaagaat gtttgtctct tagaagccaa tcaggttgaa gaattagggt ttgagataga
     1861 agttttataa aaaagtaaaa aataaataac aaaaacaaac acaaactgtt gacttcttca
     1921 aacaatagta gtatattatg tttgtgagga ctaattgact gttttttaaa gacagccaat
     1981 acactgctgg aatccagcat agaaaaaata aaaaggagta tacaatatgt ctattgttat
     2041 cggtgcagat gctgcaggtt tgagattgaa agaagttgtg aaagacttct tagaaaaaga
     2101 aaacttccac cttgtggatg ttacagctga aggtcaagac tttgttgatg tgactctcgc
     2161 tgttgctgca gaagtaaaca aagaagaaca aaaccttggt atcgtgattg atgcttatgg
     2221 agctggtcca tttatggttg caactaagat caaaggaatg gttgctgcag aagtatctga
     2281 cgaacgttca gcttatatga ctcgtggcca caacaactca cgtatgatca ctatgggggc
     2341 acaacttgtt ggtgatgaat tggctaaaaa catcgctaaa ggatttgtta atggtaaata
     2401 cgacggtggt cgtcaccaaa tccgggttga catgttgaac aaaatgggct aattagatta
     2461 tagtaagaaa ggtaagttaa aaatgagaat tgcaattgga tgtgaccaca tcgtaactga
     2521 tgaaaaaatg gcggtttcag aatttttgaa atcaaaagga tatgaagtca ttgactttgg
     2581 tacatatgac catacacgga ctcactaccc aatctttggt aaaaaagtag gggaagctgt
     2641 aactagcggc caagctgatc ttggggtatg tatctgtggt actggtgttg gtatcaacaa
     2701 cgctgtaaat aaagttccag gtgttcgttc tgccttggtt cgtgatatga caacagccct
     2761 ttatgctaaa gaacaattga acgctaacgt tatcggcttt ggtggtaaga ttactggtga
     2821 attgcttatg tgtgatatca tcgaagcttt cattcatgct gaatacaaac catctgaaga
     2881 aaacaaaaaa ttgattgcga aaattgaaca ccttgaaagt cacaatgctc aacaaacaga
     2941 cgcaaacttc ttcactgaat tccttgaaaa atgggatcgc ggagaatacc acgactaaga
     3001 ggtaacctat gattttaaca gttacaatga acccatccat tgatatttcc tatcccttgg
     3061 atgagttgaa gattgatact gtcaatcgtg tggtggatgt aaccaaaacg gctggtggta
     3121 agggactcaa tgttacccga gtactttcag aatttggcga ttctgttctt gctactggtt
     3181 tagtgggtgg caaacttggt gagtttttgg ttgaacatat cgataatcaa gtaaagaaag
     3241 atttcttctc aattcaggga gaaactcgta actgtatcgc tattctccac ggagacaacc
     3301 aaacagaagt tcttgaaaaa ggtcctgaag tattggaaca ggaaggtcaa gactttttgg
     3361 aacatttcaa aaaactcttg gagtcagttg aagtagtggc catctcaggt agtctgccag
     3421 ctggccttca agttgattac tatgcgagct tggtagaact tgctaatcaa gctggcaagc
     3481 ctgtagtctt ggactgctca ggtgcagcac ttcaggctgt tcttgaatca ccccataaac
     3541 caacagtcat caaaccaaat aatgaagaat tgtctcagct tcttggaaga gaagtttctg
     3601 aggatttgga ggaattaaaa gaagtacttc aagaaccttt gtttgcaggg attgaatgga
     3661 ttatcgtttc acttggtgcc aacggtactt ttaccaaaca tggtgacact ttctacaagg
     3721 tagatattcc tagaattcag gtggtaaatc ctgttggatc tggagactct actgtggcag
     3781 gaatttcttc aggacttctt cacaaagaat cggatgcaga attactcatc aaggcaaatg
     3841 tccttggtat gctcaatgct caagaaaaaa tgactggtca tgtcaacatg gccaactatc
     3901 aagctctata tgatcaatta atagtaaaag aggtataaaa tggctttaac agaacaaaaa
     3961 cgtgtacgct tagaaaaact ttctgatgaa aatggtatca tctcagctct tgcatttgac
     4021 caacgtggtg ctttgaaacg cctcatggct caacaccaaa cagaagaacc aactgtggcc
     4081 caaatggaag aacttaaagt tttggtagca gatgaattga ctaaatacgc ttcatctatg
     4141 cttcttgacc ctgagtatgg acttccagca actaaagctc ttgatgaaaa agctggtctt
     4201 ctccttgctt atgaaaaaac aggttatgac acaacaagca caaaacgctt gccagactgc
     4261 ttggatgtct ggtctgcaaa acgtattaaa gaagaaggtg cagatgcagt taaattcttg
     4321 ctttactatg atgtagatag ctcagacgaa ctcaatcaag aaaaacaagc ctacatcgaa
     4381 cgcatcggtt ctgagtgtgt ggctgaagat atcccattct tccttgaaat ccttgcttac
     4441 gatgaaaaaa ttgcggatgc aggttctgta gaatacgcga aagtaaaacc acacaaagtt
     4501 atcggcgcta tgaaagtctt ttcagaccca cgctttaaca ttgatgtctt gaaagttgaa
     4561 gttcctgtta acattaaata tgttgaaggc ttcgctgaag gtgaagtagt ttatacacgt
     4621 gaagaagcag cagccttctt caaagcgcaa gatgaagcaa cgaacttgcc atacatctac
     4681 ttgagtgctg gtgtatcagc taaactcttc caagatactc ttgtatttgc tcatgaatca
     4741 ggtgcaaact ttaacggagt tctttgtggc cgtgctacat gggcagggtc agttgaagct
     4801 tacatcaaag atggtgaagc agcagctcgc gaatggcttc gcacaactgg atttgaaaac
     4861 attgatgagc tcaataaagt tcttcaaaca acagcgactt catggaaaga acgtgtgtaa
     4921 gaaagtcctc ctagtttagg aacatgaatc taaaaaaatt taaaaaaagt tgtatgtaaa
     4981 ggtttacaaa ataacttact tgtgctatac ttaaatcaca agttaataca aggtgagtgt
     5041 tactaagtaa tattaggcat gatcacaggt gaattagaaa tcagctgatt ttctagttca
     5101 tttgtggtca ttttttgtac tcatatacct ttaaaatata aaaggaggtt gacatgtatc
     5161 gaattctaaa tccaatgaat cacaatgtct cgcttgtcag aaatgataag ggagaagagg
     5221 tgattgtaat tggtaagggg attgcattcg gaaaaaggaa gggggatttg attgctgaaa
     5281 atcaggttga gaaaatcttt cggatgaaga ccgaagagtc cagagaaaac tttatggctc
     5341 ttctcaaaga tgttccgctt gattttatca cagtgaccta tgaaatcatt gataagctat
     5401 caaagaaata tcattatccg attcaagagt atctctatgt aaccttgaca gatcatattt
     5461 actgttctta tcaagctcta actcaaggaa ggtacaagga tagtaatctg ccggatattt
     5521 ccgctaagta tcctgtcgct tttcaaatcg caaatgaagc ttttgaaatt taccgtcaga
     5581 agctagcaga tcattttcct gaggacgaga ttattcggat tgcttatcat ttcatcaatg
     5641 ccgaaggtga aaatgaagtg gaacttgtgg agtcgattga taagaggaaa gaaattctca
     5701 ggaatgttga agaagtgcta aagggttatg caattcaacg aaccaaaaaa aataatcatt
     5761 tctatgatcg ctttatgatt catttgaatt atttcttgga ttatttagac agatctagag
     5821 atgataacca atcacttctg gatatggaag atcatattaa acaatcctat ccaaaagcct
     5881 tcgagattgg ttccaagatc tatgatgtga ttacgcaaca tacgggtctt gatttgtata
     5941 aaagtgaacg agtttatcta gttctacata tccaacgttt attgtcataa aaatttattt
     6001 aaaactatat aaggagaatt ctatcatgaa tagagaagaa gtaacattgt taggttttga
     6061 aatcgtagcc tatgctggcg atgctcgttc aaaactattg gaagccttga aggctgctga
     6121 agctggtgat tttgaaaaag cggacgctct ggtagaggaa gctggtagct gtattgcaga
     6181 ggctcaccac gcgcaaacaa gtctattgac taaggaagct tctggtgagg acttagcgta
     6241 tagtgtgacc atgatgcatg gccaagacca cttgatgaca actatcttgt taaaggattt
     6301 gatgcaccat ttgatcgaac tctataagag aggagttaaa taatgaacaa actaattgca
     6361 tttatcgaga aaggaaagcc tttctttgat aaactatctc gtaatatcta tcttcgtgct
     6421 attcgtgatg gtttcattgc aggtatgcct gttattctct tctcaagtat ctttatcttg
     6481 attgcctttg taccaaactc atggggcttt aaatggtctg atgaagttgt agcctttctg
     6541 atgaaacctt atagctattc tatgggtatt ctggctctct tggtagctgg tacaacagct
     6601 aagtcattga ccgactcagt aaaccgtagc atggagaaaa ccaatcaaat caactatatg
     6661 tcaacgctgt tagcagcgat tgttggtttg ttgatgttgg cagctgatcc tatcgaaaat
     6721 ggtctagcta ctggattctt ggggacaaaa ggtttgcttt cagccttcct tgctgccttt
     6781 gttactgtag ccatctataa ggtctgtgtt aagaacaacg tcactattcg tatgcctgac
     6841 gaagttccac caaatatctc acaagtcttt aaagatgtga ttccattcac tctatcagtg
     6901 gtttctcttt atgctcttga tttactagct cgtcattttg ttggttctag tgtggcagaa
     6961 tcaatcggta aattcttcgc accactcttc tcagcagcag acggatacct tggtattacc
     7021 attatctttg gtgcctttgc cttcttctgg tttgtgggta ttcatggtcc atctattgtt
     7081 gaacctgcta tcgcagcgat tacctatgca aatgctgaag tcaacttgaa ccttctccaa
     7141 caaggtatgc atgctgataa aatccttact tcaggaactc aaatgtttat cgttactatg
     7201 ggaggtactg gtgcgacact ggttgttcca ttcatgttca tgtggttaac aaaatcaaaa
     7261 cgtaaccgtg caatcggacg tgcttcagta gttccaactt tctttggtgt aaatgaacca
     7321 atcttgtttg gtgcaccact tgtattaaat ccaatcttct tcattccatt tatctttgct
     7381 ccaatcgcaa acgtatggat tttcaaattc tttattgaaa ctcttggaat gaactcattc
     7441 actgctaatc taccatggac aactccagct ccactaggtc tagttcttgg aactaacttc
     7501 caagtgctat cattcattct tgctgccctt ctaatcgtgg ttgacgttgt catttactat
     7561 ccattcctta aggtctatga tgaacaaatt cttgaagaag aacgttcagg taagtctaat
     7621 gatgaattga aagaaaaagt tgctgcaaac ttcaacactg caaaagcgga tgctattctt
     7681 gaaaaagcgg gtgtcgatgc agcacaaaat accatcactg aagaaacaaa tgtcctcgtt
     7741 ctctgtgcag gtggaggaac aagtggtctc cttgcaaatg ctctaaacaa ggcagcagca
     7801 gaatacaatg tccctgtgaa agcagcagca ggcggctatg gtgctcaccg tgaaatgtta
     7861 ccagagtttg atcttgttat ccttgcccct caagttgctt caaactttga agatatgaaa
     7921 gcagaaacag ataagctcgg tattaaacta gcgaaaacag aaggcgctca atacatcaaa
     7981 ttaactcgtg atggaaaagg tgctcttgca ttcgtacaag cgcaattcga ttaaggctag
     8041 agactctgaa atagtctcct atcgttacgg aaatcgctat ggcgaatttc ctattattaa
     8101 ttcgtcggta aaaagatatc gtttttacct cctcatgtca caattcggtg acttggtaca
     8161 agaagtgaga tggagaagga tggctcactg actcctctcc tctcactttt actttattta
     8221 aatcaagaaa taggtgaaaa aaatgacaaa aacacttcca aaagacttta tttttggtgg
     8281 cgcaacagct gcctatcaag cagaaggtgc tacacatact gatggtaaag gaccagtagc
     8341 gtgggataaa tatcttgaag ataactactg gtacactgca gaaccagcca gtgattttta
     8401 caatcgatat ccagttgatc tcaagctagc agaagagtat ggtgtcaatg gcattcgaat
     8461 ttctattgcc tggtcacgta ttttcccgac tggttatggt caagtaaatg ctaaaggtgt
     8521 tgagttttat cataatttat ttgcagagtg tcacaaacgt catgttgagc cctttgtaac
     8581 tcttcatcac tttgacacgc cagaagctct ccactcaaat ggagacttct taaaccgtga
     8641 aaatatcgaa cattttgtag actacgctgc cttctgtttt gaagaatttc cagaagtaaa
     8701 ctattggaca accttcaatg aaattggacc aatcggtgat ggtcaatatt tggttgggaa
     8761 attccctcca ggtatccagt acgaccttgc caaagtcttt caatcacacc acaatatgat
     8821 ggtgtctcat gcatgcgcgg taaaattgta caaagagaaa ggctataaag gggaaattgg
     8881 tgttgttcac gccctgccta ctaaatatcc tctagatcct gaaaatccag cagatgttcg
     8941 tgcagcagag ttggaagata tcatccacaa taaattcatc ttagacgcaa cttatctagg
     9001 tcgctattca gctgaaacca tggaaggtgt caaccatatc ttatcagtca atggtggtag
     9061 tttggatctt cgtgaagaag attttacagc attagaagct gcaaaagact tgaatgattt
     9121 cctaggaatc aactactata tgagtgactg gatggaagcc tttgatggag aaactgaaat
     9181 tatccataat ggtaaaggtg aaaaaggaag ctctaagtat caaatcaaag gtattggtcg
     9241 tcgtgtagct cctgactatg tatcacgcac ggattgggat tggattatct accctcaagg
     9301 tttgtatgac caaatcatgc gtgtgaagaa agattatcct aactacaaga aaatttacat
     9361 cactgaaaat ggtctcggct ataaagatga gttcgttgat aacactgttt acgatgatgg
     9421 tcgtattgat tacgtgaaac aacacttgga gatcttgtct gatgcgattg cagatggagc
     9481 taatgtaaaa ggttacttca tttggtcatt aatggatgtc ttctcatggt caaacggtta
     9541 tgagaaacgt tatggtctct tctacgtaga ttttgaaact caagaacgtt atcctaagaa
     9601 atcagctcac tggtacaaga aagtagcgga aactcagatt atagactagt agaattagtc
     9661 attagatata gaattttagt gagtcaaaaa gatgttcaaa gattttatcc aatctattta
     9721 tgaaaaaaag tttatattat aaatttcgaa aaatgctctc aaataccgtg tttgacgagt
     9781 gaagaattga aaagtcttgg aaaatggtat gtctcgactg gtaaagaatg ggatactatc
     9841 tcctttgatt cagattttat gccgtttcaa caatcgtaac caatttctca aaaaagttaa
     9901 atcttatatt tagtactctg taaaactctt atctaatcac gttgcttata ctcaatgaaa
     9961 atcaaagagc aactttaaac taggaagcga gtcgcaaatt tctcaatgca tagctttgag
    10021 gaattgggca aaaagtcttt gatatagaaa aaagcatagt atcaggtgtt gaaacacctg
    10081 atactatgct ttttattgtg ggaagattta ctttttttct tctgaaattg agttgttacc
    10141 caggctcttt cagtttatta aggcttgatg actttaatgt gtttagatag cttaaaaagg
    10201 attgaatcac ttagtttaga a
//
LOCUS       DAVLVP010000065         9990 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.065, whole genome shotgun sequence.
ACCESSION   DAVLVP010000065 DAVLVP010000000
VERSION     DAVLVP010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9990
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.065"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(97..1800)
                     /gene="ilvD"
                     /locus_tag="VYN69_001515"
     CDS             complement(97..1800)
                     /gene="ilvD"
                     /locus_tag="VYN69_001515"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HEV1533765.1"
                     /translation="MTELDKRHRSSIYDSMVKSPNRAMLRATGMTDKDFETSIVGVIS
                     TWAENTPCNIHLHDFGKLAKEGVKSAGAWPVQFGTITVADGIAMGTPGMRFSLTSRDI
                     IADSIEAAMSGHNVDAFVAIGGCDKNMPGSMIAIANMDIPAIFAYGGTIAPGNLDGKD
                     IDLVSVFEGIGKWNHGDMTAEDVKRLECNACPGPGGCGGMYTANTMATAIEVLGMSLP
                     GSSSHPAESADKKEDIEAAGRAVVKMLELGLKPSDILTREAFEDAITVTMALGGSTNA
                     TLHLLAIAHAANVDLSLEDFNTIQERVPHLADLKPSGQYVFQDLYEVGGVPAVMKYLL
                     ANGFLHGDRITCTGKTVAENLADFADLTPGQKVIMPLENPKRADGPLIILNGNLAPDG
                     AVAKVSGVKVRRHVGPAKVFDSEEDAIQAVLTDEIVDGDVVVVRFVGPKGGPGMPEML
                     SLSSMIVGKGQGDKVALLTDGRFSGGTYGLVVGHIAPEAQDGGPIAYLRTGDIVTVDQ
                     DTKEISMAVSEEELEKRKAETTLPPLYSRGVLGKYAHIVSSASRGAVTDFWNMDKSGK
                     K"
     gene            complement(2033..2965)
                     /locus_tag="VYN69_001516"
     CDS             complement(2033..2965)
                     /locus_tag="VYN69_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HEV1533766.1"
                     /translation="MMRSTKELRHVYRDFLLEANQSYSDIVVLEADLSSSMATHNLEK
                     NFGDRYVNVGIMEAEMVDLAAGLSIQGFRPYLHTFGPFASRRVFDQLFISLGYAQLDA
                     TVIGSDAGVTAEMNGGTHMPFEEIGLLRLIPKSTIFEATDDIQFREILNQTLDLKGLK
                     YIRTIRKAPVPVYQGGEDFSKGYIELRHGEDLVIVASGIMVAPSIRVADELSKLGYSV
                     GVIDLFRIKPIPEQIKTMLSGKTIFTVENHNQIGGIGSALCELFSDDGITKIHRMGVQ
                     ERFGQVGKMDYLLNEYGLSESNIKEKVLEIYNAR"
     gene            complement(2962..3819)
                     /locus_tag="VYN69_001517"
     CDS             complement(2962..3819)
                     /locus_tag="VYN69_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEV1533767.1"
                     /translation="MILSENREDELRKFATNIRLNTLRTLNHLGFGHYGGSLSIVEVL
                     AVLYGEIMPMTPEIFAARDRDYFILSKGHGGPALYSTLYLNGFFDKEFLYSLNTNGTK
                     LPSHPDRNLTPGIDMTTGSLGQGISVATGLAYGQRIRKSPFYTYAIVGDGELNEGQCW
                     EAIQFASHQQLSNLIVFVDDNKKQLDGFTKDICNPGDFVEKFSAFGFESIRVKGSDIR
                     EIYEGIVQLKQSNNSSPKCIVLDTIKGQGVQELEEMKSNHHLRPTVEEKQMLTSVVER
                     LSQELEETE"
     gene            complement(3823..5169)
                     /locus_tag="VYN69_001518"
     CDS             complement(3823..5169)
                     /locus_tag="VYN69_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1533768.1"
                     /translation="MMKFILDIVSTPAILVALIAILGLVLQKKKLPDIIKGGIKTFVG
                     FLVVSGGAGIVQNSLNPFGTMFEHAFHLSGVVPNNEAIVAVALTTYGSATAMIMFAGM
                     VFNILIARFTRFKYIFLTGHHTLYMACIIAVILSVAGFTSLPLILLGGLALGIIMSIS
                     PAFVQKYMVQLTGNDKVALGHFSSLGYWLSGFTGSLIGDKSKSTEDIKFPKSLAFLRD
                     STVSITLSMAVIYIIVAIFAGSEYIEKEISSGTSGLVYALQLAGQFAAGVFVILAGVR
                     LILGEIVPAFKGISERLVPNSKPALDCPIVYTYAPNAVLIGFISSFIGGLVSMAIMIA
                     SGTVVILPGVVPHFFCGATAGVIGNASGGVRGATIGAFLQGILISFLPVFLMPVLGGL
                     GFQGSTFSDADFGLSGIILGMLNQFGSQAGIVIGLVLILAVMFGVSFIKKPSAKEE"
     gene            complement(5182..5466)
                     /locus_tag="VYN69_001519"
     CDS             complement(5182..5466)
                     /locus_tag="VYN69_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008089859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1533769.1"
                     /translation="MLKIGTACGSGLGSSFMVQMNIESVLSDLNVSDVEVEHYDLGGA
                     DPNAADIWIVGRDLADSASHLGDVRILNSIIDMDELRELITKLCEEKGLI"
     gene            complement(5470..7500)
                     /locus_tag="VYN69_001520"
     CDS             complement(5470..7500)
                     /locus_tag="VYN69_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000460919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1533770.1"
                     /translation="MFDYRALVILMTLMDHCELSLYELSVKVSLPIKEVKEGIDYLVP
                     YLANKGIVLDKKQGRYSLSNRTKQSLTDIIKSDELVLPKSTRLALIYLYTFCRLDFIS
                     NNHYQDFLKVSKNTTLTDIQSLRKIMLDNDLELGYSRAKGYTLHGSEWNKHRLAFQMV
                     SELLESSIGIWGLDYVLSSWGYSLTYDLINQVVKDYYEKLQLVPIVNQLKVCLFGLVF
                     ILCRYQRDVERVCLSETLVSPVIQDITTILLDTVVDLGIIDTVFSEDDYRYITVLLSS
                     CFEGEVDVAPVYFNQLTEAIISRMEDISLLHFKQREGLRENLRRHLIPAYYRLKFGLP
                     SSNEYVLHVKEHYPDLFELVKDSLMPLMDAIDNPIPDSETAYFVIHFGGYLKKADTLP
                     QKWYKAAIICPNGVSSSLMLKENLLALFPQIEFIGTSKIDDLQVKASSDYDMVFSTIK
                     VETEKPNYLVSVMMTEEQAIQLVELVLKDFPNLEYGDFEIEQILNIVKRYGIITQELE
                     LRLALKNYLYQKNDRKEIVPVLEQLITKETYQVSSQKLGWKEAIRLAAKPLLDQDKIT
                     ENYPEAMIQKVEEFGPFINLGKGVAIPHARPDEGVNEIGMSMLVLEEPIYLLDNPEQE
                     VRLLICIAAIDNESHLKALSHLTTILRDKNHVQTLISSKNYDDIKMIIKQED"
     gene            7692..8699
                     /locus_tag="VYN69_001521"
     CDS             7692..8699
                     /locus_tag="VYN69_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEV1533771.1"
                     /translation="MTEKLINSKPNGVFALILIELTIVLGIFIFIMGVGSENIFGIII
                     GPLLIVIAGLAHAGLKVVKPQEALVLTLFGNYTGTIKEPGFYFVNPFSVAVNPANHTR
                     LGQSGDVSTKSPFLGAKSSNGNDVNLEIGKKHISLKVMTLSNSRQKINDCLGNPVEIG
                     IAVTWRVVDTAKAVFNVDNYKEYLSLQCDSALRNIVRIYPYDVSPNVDTTGDGQADEG
                     SLRGSSEIVANRIREEIQSRVEDAGLEILEARITYLAYAPEIAAVMLQRQQASAIIDA
                     RKMIVDGAVGMVEMALERLNEGELVELDEERKAAMVSNLLVVLCGNHDAQPIVNTGSL
                     Y"
     gene            8702..8902
                     /locus_tag="VYN69_001522"
     CDS             8702..8902
                     /locus_tag="VYN69_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEV1533772.1"
                     /translation="MAEAKKKQIPLRLSTKLYAALASWAEDDFRSVNGQIEYLLTECV
                     KQRKKDGKYVSETIDEPFEIDI"
     gene            9054..9236
                     /gene="rpmF"
                     /locus_tag="VYN69_001523"
     CDS             9054..9236
                     /gene="rpmF"
                     /locus_tag="VYN69_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000290417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEV1533773.1"
                     /translation="MAVPARRTSKAKKNKRRTHYKVTAPSVNFDETTGDYSRSHRVSL
                     KGYYKGRKIAKAASAE"
     gene            9252..9401
                     /gene="rpmG"
                     /locus_tag="VYN69_001524"
     CDS             9252..9401
                     /gene="rpmG"
                     /locus_tag="VYN69_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1533774.1"
                     /translation="MRVNITLEHKESGERLYLTSKNKRNTPDRLQLKKYSPKLRKHVV
                     FTEVK"
     gene            complement(9881..>9990)
                     /locus_tag="VYN69_001525"
     CDS             complement(9881..>9990)
                     /locus_tag="VYN69_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase family
                     protein"
                     /protein_id="HEV1533775.1"
                     /translation="TVSGKWYYTYNSGDLLVNTTTPDGYRVNANGEWVG"
BASE COUNT         3242 a         2108 c         1618 g         3022 t
ORIGIN      
        1 actgttttga ggttgtggat agaactgacg aagtcagtaa ccatacctac ggcaaggcga
       61 cgttgacgtg atttgaagag attttcgagt atgagtttat tttttacctg acttgtccat
      121 attccagaag tctgtcacgg ctccgcgtga agcagatgat acgatgtggg catatttacc
      181 gaggacacca cggctgtaaa gtggtggcaa ggttgtttct gccttgcgtt tttcaagttc
      241 ttcttcggat acagccatgg aaatttcttt ggtatcttgg tcaaccgtaa cgatatcgcc
      301 ggtacggaga taggcaattg gtccaccatc ctgagcttca ggagctatat gtccaacaac
      361 cagaccataa gtaccaccag agaaacgtcc gtccgtcaag agggccacct tatctccctg
      421 acctttacca acaatcattg aagaaagtga tagcatctca ggcataccag gaccaccttt
      481 aggtccaaca aaacgaacaa cgactacatc gccatcaacg atttcatctg tcagaacggc
      541 ctgaatcgca tcttcttctg agtcaaagac cttagctggt ccaacgtgac gacgcacttt
      601 aacacctgat accttggcaa ctgcaccgtc aggagcaagg ttcccgttca agatgataag
      661 cggaccatcc gcacgttttg gattttcaag tggcatgata actttttggc ctggagtcaa
      721 gtctgcaaag tcagccaagt tttcagctac agtcttacca gtacatgtga tgcgatctcc
      781 gtgaaggaaa ccatttgcca acaagtactt cataaccgca gggacaccac cgacttcgta
      841 gaggtcttgg aagacatact gaccagatgg tttcaagtcg gccaagtgag gcacacgttc
      901 ttgaatcgta ttgaagtcct caagtgacaa gtcaacattt gcggcatggg caatggcgag
      961 caagtgaaga gtggcgtttg tagaaccacc gagagccatc gttacagtaa tagcatcttc
     1021 aaaggcttca cgagtcaaga tatctgatgg tttgagacca agttccaaca tcttaacaac
     1081 agcacgtcct gctgcttcga tatcttcttt cttatcagct gattcagctg ggtgagagga
     1141 tgaccctggc aaactcatcc ctagaacttc gatagcagtt gccatggtat tagcagtata
     1201 cataccacca caaccaccag ggccagggca ggcattacat tcaagacgtt tcacgtcctc
     1261 agctgtcatg tcaccgtggt tccattttcc gataccttca aagacagaaa ccaagtcgat
     1321 atctttacca tcaagatttc ccggtgcaat agttccacca taggcgaaaa tagctgggat
     1381 atccatatta gcaatagcaa tcatagatcc aggcatgttc ttgtcacagc caccgatagc
     1441 gacgaaggca tccacgttgt gaccactcat agccgcctcg atggagtccg cgatgatgtc
     1501 acgagatgtt agagagaaac gcataccagg cgttcccata gcgatcccgt ctgctacggt
     1561 aatggttcca aactgtacag gccaagcgcc tgcagatttg acaccttctt tagccagttt
     1621 cccgaaatca tgcaagtgaa tgttacatgg tgtattttcc gcccaagtcg aaatcactcc
     1681 cacaatcgat gtttcaaagt ccttatctgt cataccagtc gcacgaagca tagcacggtt
     1741 aggtgattta accatgctgt catagatgct actgcggtga cgtttatcta attcagtcat
     1801 cttatccctc ccatttcagt ttttactatt atagcacaat tttcgcatga agaacagaat
     1861 aaaaatcttg aattttcaga aaattctata cacatgtgaa atatttaaaa ttaaaaacaa
     1921 caaagcggat tagtgcactt tctgatgacc agaatatgct ttttaatccg ctttctttaa
     1981 ataacgtact gtaattttta cagaaattct ttcagataag tgtatttaac atctatcttg
     2041 cattataaat ttctagaacc ttctctttta tattcgattc actcaaacca tactcattaa
     2101 gaagataatc cattttccct acttgaccga atctttcttg aacacccatc cgatgaattt
     2161 ttgttattcc atcatcagag aataattcac ataaagcact gccaattcca cctatctgat
     2221 tgtggttttc tacagtaaat atagtttttc cacttaacat tgtttttatc tgttctggta
     2281 tcggtttgat tctaaataaa tctatcacac ctactgaata acctaattta gacagttcat
     2341 ctgcaactcg aatacttgga gcaaccatta taccagaagc aacgattaca agatcttccc
     2401 catgtcttaa ctcaatgtag cctttagaaa aatcttctcc accttgatac acaggaactg
     2461 gagcttttct aattgttcga atatatttta gtccttttaa gtctaatgtc tggttcaata
     2521 tttcacgaaa ttggatatca tcagttgctt caaaaatggt cgatttagga attaaacgta
     2581 acaatccaat ttcttcaaat ggcatatgtg tcccaccatt catttctgca gttactcctg
     2641 catctgatcc aatcacagtg gcatccaatt gtgcgtatcc aagagaaata aataattgat
     2701 caaataccct tcgtgaagca aaaggaccaa atgtatgaag ataaggtcta aacccctgaa
     2761 tagacaagcc tgctgcaagg tcgaccattt ctgcttccat aatcccaaca ttcacataac
     2821 ggtctccaaa gttcttttca agattatgag tagccatcga acttgacaaa tcggcttcta
     2881 agactactat atcagaataa ctttgattag cctctaaaag gaagtctcta tatacatgcc
     2941 gtaattcttt cgtacttctc atcattctgt ttcctccaat tcctgactta atctttctac
     3001 aactgaagtt aacatttgtt tctcctctac agtagggcga agatgatgat tggatttcat
     3061 ttcttccagc tcttgaaccc cttgaccttt aatagtatct aatacaatgc acttaggtga
     3121 tgaattattt gactgtttta attggacaat cccttcataa atttctctaa tatctgaacc
     3181 cttgacccta atggattcaa atccaaatgc tgaaaatttt tctacgaaat cacctggatt
     3241 acaaatatcc tttgtaaaac catctaattg ttttttgtta tcatcaacaa atacaattaa
     3301 gttggataac tgttgatgag aagcaaactg tatagcctcc caacattgtc cctcatttaa
     3361 ctcaccatct ccaacaatag cataagtata aaagggactc tttcttattc tctgaccata
     3421 tgcaagtcca gttgcaacac taattccttg tcctaaagag cccgttgtca tatctatgcc
     3481 tggcgttaga tttctatcag gatgagacgg taatttggtt ccatttgtat ttaaagaata
     3541 taagaattct ttgtcaaaga aaccattcaa atagagtgta ctgtatagag ctggtcctcc
     3601 atgacctttt gataatatga aataatctct atctcgtgct gcaaatattt ctggagtcat
     3661 tggcattatt tcaccataaa gcactgctaa aacttctacg atagacagac ttcctccgta
     3721 atgtccgaat ccaagatgat tcaatgttct aagagtattt aatcggatgt tagtcgcaaa
     3781 ttttcttaac tcatcttctc tattttcact taaaatcatc ccttactcct cctttgcaga
     3841 tggcttttta ataaaggata ctccaaacat aactgctaga ataagaacaa gaccaatcac
     3901 aatgcctgct tgtgagccaa attgatttaa cattcctaaa ataattcctg atagaccaaa
     3961 atctgcatct gagaaagttg atccttggaa accaagtcct cccaaaactg gcattaaaaa
     4021 gactggaaga aaactgatta aaataccttg taaaaatgct ccaatagtgg ctccacgaac
     4081 accaccagat gcattcccaa tgacacctgc agtcgctcca cagaagaaat gaggcacaac
     4141 acctggtaag ataacaaccg ttcctgaagc aatcataatt gccatactta ctaaaccacc
     4201 aataaaacta gagataaatc caattagaac tgcattgggt gcataagtat aaacaatcgg
     4261 acaatccaaa gcaggttttg aattaggtac aagacgctct gaaatacctt taaaggctgg
     4321 aacaatttca cccaaaataa ggcgaacacc tgctaaaata acaaataccc ctgctgcaaa
     4381 ttgacctgct aattgtaaag cataaactag accacttgta ccactactga tttctttttc
     4441 tatatattct gaccctgcaa agatagctac aataatgtaa ataactgcca tagataaagt
     4501 gatactaaca gtactatcac gtaaaaaagc taaactcttt ggaaatttaa tgtcctctgt
     4561 tgattttgat ttgtcaccga taaggctacc agtaaaacca ctcaaccaat atcccaaaga
     4621 actgaaatga cctaaagcca ccttgtcatt tccagttaat tgaaccatat atttttgcac
     4681 aaatgctggg gaaatactca taataatacc gagtgctaat cctcctagta agatgagagg
     4741 caagctagta aagccagcaa ctgataaaat gaccgcaatt atacatgcca tatatagagt
     4801 gtggtgccct gttaaaaaga tatatttaaa tcgagtaaaa cgagcgatta agatattgaa
     4861 caccatgcct gcaaacataa tcattgcagt agctgagcca tatgttgtta aagctacagc
     4921 tacaattgct tcattattcg gcacaacgcc agataaatga aaagcatgct caaacatggt
     4981 accaaatgga tttaaagaat tttgtacaat tcctgcacca ccagatacaa ctaagaaacc
     5041 aacaaaggtc ttaattccac ctttaataat atcaggtaat ttcttcttct gaagaactaa
     5101 tcctaaaatt gcaattaaag ctactaaaat agctggtgta ctaacaatat ccaatatgaa
     5161 cttcatcatg acgctagcct cctatataag tcctttttct tcacaaagtt tagtaattaa
     5221 ttctcgtagt tcatccatat caataatact atttaagata cgaacatctc caagatgact
     5281 agctgaatca gctagatcac gaccaacaat ccaaatatca gcagcatttg gatctgctcc
     5341 acctaaatca taatgttcaa cttctacatc cgaaacattc aaatcactca atacagattc
     5401 aatattcatc tgtaccataa aacttgaacc taatcctgaa ccacaagctg taccaatttt
     5461 taacattatc taatcctcct gtttaattat cattttaatg tcatcatagt tttttgatga
     5521 aattaaagtt tgaacatgat ttttatctct taaaattgtt gttaaatgtg acaaagcctt
     5581 taaatgactc tcattatcaa tggctgcaat acaaatcaac aatcttacct cttgttctgg
     5641 attatccaat aaataaatcg gttcttccaa aactaacatt gacattccta tttcattcac
     5701 accttcatct ggccgagcgt gaggaattgc tactcccttc cctaaattaa taaaaggtcc
     5761 aaactcttct actttttgaa tcattgcctc agggtagttc tccgttatct tatcttgatc
     5821 caaaagcggt ttagctgcta aacgaatcgc ctccttccat cctaattttt gcgaactaac
     5881 ctgataggtt tctttggtaa taagttgttc tagcactggt acaatttcct ttctatcatt
     5941 tttttgataa agataattct ttaacgccaa tcttaattcc aattcttgtg taataattcc
     6001 atatcttttg acaatattca ggatttgttc aatctcaaaa tctccatact ctaaattcgg
     6061 aaaatctttt aacactagtt ctactagttg tattgcttgc tcttcagtca tcataaccga
     6121 aactagataa tttggctttt ctgtctccac ctttatggta gaaaaaacca tatcatagtc
     6181 actactagct ttcacctgta aatcatcaat ctttgaggtt cctataaact caatttgagg
     6241 aaataatgct aatagattct cttttaacat caatgaagaa ctaacaccat taggacaaat
     6301 gattgctgct ttataccatt tttgaggcaa agtatctgct ttctttaaat aacctccgaa
     6361 atggataaca aaatatgctg tttcactatc aggtatggga ttgtcaatag cgtccatcaa
     6421 gggcatcaaa gaatctttga ctaattcaaa taaatcagga taatgttctt taacatgcaa
     6481 tacatattca tttgaactag gtaggccgaa ctttaatcta tagtaagccg gtataaggtg
     6541 gcggcgaaga ttttctctca atccttccct ttgtttaaaa tgtaacaaag aaatatcttc
     6601 cattctactt ataatagcct ctgttaattg attaaagtaa accggagcaa catctacttc
     6661 accttcaaag caacttgata ataaaactgt gatatagcga taatcatcct cagaaaacac
     6721 cgtatctata attcccaaat caaccactgt atccaataaa atagtggtta tatcttgaat
     6781 aacaggagat actaatgtct ctgaaagaca tactctttca acatcccttt gatacctaca
     6841 cagaatgaat actaaaccga aaaggcaaac ttttaattga ttaacaatag gtactagctg
     6901 tagcttctca taataatctt taactacctg atttatcaaa tcataagtta atgaataccc
     6961 ccaactggat aaaacataat ccaaacccca aatccctatg gaggattcca gcaactcact
     7021 aaccatttga aaagctaaac ggtgcttatt ccactctgaa ccgtgtaaag tataaccttt
     7081 tgctctactg taccctagct ccaaatcatt atctaacata atctttctta atgattgaat
     7141 atcagttaag gttgtattct tacttacttt caaaaagtct tggtaatgat tattcgatat
     7201 aaaatctaat cggcaaaaag tgtaaagata gattaaagct aagcgagtcg actttggtaa
     7261 aaccaattca tccgacttaa taatatctgt caaagactgc ttcgtacgat ttgataaact
     7321 atagcgacct tgttttttat ccagcactat ccctttatta gctagataag gcactaaata
     7381 atctattcct tctttgactt cctttatagg taagctcacc ttaacagata attcatataa
     7441 tgatagctca caatgatcca tcaaagtcat caaaataact agtgctctat aatcaaacat
     7501 ttctcttcct ttctaactac aattatagtg taaaaattat aagaataaaa gagtatttga
     7561 gcacaatata atctcacctt ttaaaagaaa ttattgggct gaaagttttt agtgattatt
     7621 tttcaacttt ctattgactt tataaattaa agtgatatca tttagatatc aaataacagg
     7681 aggtcattct tatgactgaa aaactaatca attcaaaacc aaatggtgta ttcgcattga
     7741 ttctcattga gttgacaatc gtacttggta tctttatatt tataatgggg gttggttcgg
     7801 aaaacatttt tggaattatt atcggacctt tactaatcgt aattgcaggt ctagctcatg
     7861 ctggtttaaa agttgtcaaa cctcaggaag ctctggttct gacactcttt ggtaattata
     7921 caggtaccat caaagaacct ggtttttact ttgtcaatcc cttcagcgta gcagtcaacc
     7981 ctgcaaacca cactcgactt ggacaaagtg gtgatgttag cacaaaatct ccttttttag
     8041 gagctaaatc atcaaatggc aatgatgtaa atcttgaaat tggcaagaaa cacatttccc
     8101 tcaaagtcat gaccttgagc aattctcgtc aaaaaatcaa tgattgctta ggaaaccctg
     8161 tagaaatcgg tatcgcggta acttggagag ttgtcgatac cgctaaggca gtcttcaacg
     8221 ttgataacta caaagaatat ctttcattgc aatgtgatag cgccctccgt aatattgtcc
     8281 gcatctatcc ttacgatgtg tctcctaatg tggatactac gggtgacggg caagcagatg
     8341 aaggtagtct ccgtggctct agtgaaattg tagctaaccg tattcgtgaa gaaatccaaa
     8401 gtcgtgttga ggatgctggc ttggaaatcc ttgaagcacg tatcacttac ctagcttatg
     8461 ctccagaaat tgctgccgtt atgcttcaac gccaacaagc atctgccatt attgatgcac
     8521 ggaagatgat tgtagatggt gctgtaggaa tggttgaaat ggcactagaa cggctcaatg
     8581 aaggggaatt ggtagaactt gacgaagaac gaaaagctgc catggtttca aatctccttg
     8641 tcgttctttg tggcaatcat gatgcacaac caattgtcaa cacaggaagt ctttactaaa
     8701 gatggctgaa gctaaaaaaa agcagatccc ccttcgactc tcaacaaagt tatacgctgc
     8761 actcgcatca tgggcagaag acgactttcg ttcagtcaat gggcaaatcg aatatctcct
     8821 cacagagtgt gtcaagcaac ggaaaaaaga cggaaaatac gtatcagaaa ccattgacga
     8881 accatttgag attgatattt aacaccttct cctgtctgcc gtgtcggata ggagaatttt
     8941 ttcatctttt tttgtcaagt aactttactt tacaaaaaaa atgtgttatc ctagtatggt
     9001 tgatgaaaat cagtagattg aatcgaatat aaatacctaa aggagaaatc aaaatggcag
     9061 tacctgcacg tcgcacttca aaagcgaaga aaaacaaacg tcgtacacac tacaaagtaa
     9121 cagctccatc tgtaaacttt gacgaaacta ctggagatta ctcacgttct caccgtgtat
     9181 cacttaaagg atactacaaa ggacgtaaaa tcgctaaagc tgcatcagct gaataataga
     9241 agggagatac catgcgcgta aatattacac ttgaacacaa agaatctggt gaacgcttgt
     9301 accttacttc taaaaacaaa cgtaacactc cagaccgcct tcaattgaag aaatactcac
     9361 caaaacttcg caaacacgtt gtgtttacag aagtgaagta agcattcaat acgaatcaat
     9421 acagaagaaa aacgcttgat cagcgttttt tctttttgtt aatttcacta tattgcaaga
     9481 gaaatcattc gaatgactac attttttgaa ataagaatac ggattcggac gattaaaagt
     9541 aagagaaact caaatgtaag ttggggttcc ttttttgtat atttttcaaa taacgcctct
     9601 acactatttg tagcaaattc accaactaca gttgcatctt agttaaaata agttagaata
     9661 tgtaagtgag taccagatat accaagacat cgtcaccatc taaggtatat tcaaaataca
     9721 aaagttgacc aactagattt ctgaatatcc ttatatatcc attcttaaaa ttggtttaaa
     9781 tagcgtagtc ttttaaacta gttttgagaa tccaaaaaat cttcctacat atgtaagaag
     9841 attttttagt tcagaatgat tagatttagc taatggatac ctatcctacc cactcaccgt
     9901 tagcattgac tcgatagcca tcgggtgtag tcgtgtttac taataaatct cctgaattat
     9961 aggtatagta ccatttacca gaaactgtaa
//
LOCUS       DAVLVP010000066         9809 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.066, whole genome shotgun sequence.
ACCESSION   DAVLVP010000066 DAVLVP010000000
VERSION     DAVLVP010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9809
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.066"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..304)
                     /locus_tag="VYN69_001526"
     CDS             complement(<1..304)
                     /locus_tag="VYN69_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanine-N7)-methyltransferase"
                     /protein_id="HEV1533776.1"
                     /translation="MSVLDEEYLKNTRKVYNDFCNQADNYRTSKDFIDNIPIEYLARY
                     REIILAEHDSCIKNDEAVRNFVTSVLLSAFVSALVSAMISLEIQTYKFVIPFIIGMI"
     gene            complement(306..2129)
                     /gene="dnaK"
                     /locus_tag="VYN69_001527"
     CDS             complement(306..2129)
                     /gene="dnaK"
                     /locus_tag="VYN69_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002946936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEV1533777.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGTESKIIANPEGNRTTPSVVSFKN
                     GEIIVGDAAKRQAVTNPDTVISIKSKMGTSEKVSANGKEYTPQEISAMILQYLKGYAE
                     DYLGEKVTKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTDK
                     EEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNKLGGDDFDQKIIDHLVAEFKKEN
                     GIDLSTDKMAMQRLKDAAEKAKKDLSGVTSTQISLPFITAGEAGPLHLEMTLTRAKFD
                     DLTRDLVERTKVPVRQALSDAGLSLSEIDEVILVGGSTRIPAVVEAVKAETGKEPNKS
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTSK
                     SQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDIDK
                     NGIVSVKAKDLGTQKEQTIVIQSNSGLTDEEIDRMMKDAEANAEADKKRKEEVDLRNE
                     VDQAIFATEKTIKETEGKGFDAERDAAQAALDDLKKAQEDNNLDDMKAKLEALNEKAQ
                     GLAVKLYEQAAAAQQAQEGAEGAQATGNAGDDVVDGEFTEK"
     gene            complement(2609..3133)
                     /gene="grpE"
                     /locus_tag="VYN69_001528"
     CDS             complement(2609..3133)
                     /gene="grpE"
                     /locus_tag="VYN69_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEV1533778.1"
                     /translation="MAQDIKNEEVEEVQEEEVVETAEETTPEKSELDLANERADEFEN
                     KYLRAHAEMQNIQRRANEERQNLQRYRSQDLAKAILPSLDNLERALAVEGLTDDVKKG
                     LAMVQESLIHALKEEGIEEIAADGEFDHNYHMAIQTLPADDEHPVDTIAQVFQKGYKL
                     HDRILRPAMVVVYN"
     gene            complement(3160..4194)
                     /gene="hrcA"
                     /locus_tag="VYN69_001529"
     CDS             complement(3160..4194)
                     /gene="hrcA"
                     /locus_tag="VYN69_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEV1533779.1"
                     /translation="MVTERQQDILNLIIDIFTKTHEPVGSKALQESINSSSATIRNDM
                     AELEKQGLLEKAHTSSGRMPSVAGFQYYVKHSLDFDRLAENEVYEIVKAFDQEFFKLE
                     DILQEAANLLTDLSGCTVVALDVEPSRQRLTAFDIVVLGQHTALAVFTLDESRTVTSQ
                     FLIPRNFLQEDLLKLKSIIQERFLGHTVLDIHYKIRTEIPQIIQRYFTTTDNVIDLFE
                     HIFKEMFNENIVMAGKVHLLNFANLEAYQFFDQPQKVALEIREGLREDQMQNVRVADG
                     QESCLADLAVISSKFLIPYRGVGILAIIGPVNLDYQQLINQVNVVNRVLTMKLTDFYR
                     YLSSNHYEVH"
     gene            complement(4358..4868)
                     /locus_tag="VYN69_001530"
                     /pseudo
     CDS             complement(4358..4868)
                     /locus_tag="VYN69_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001088691.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2812 domain-containing protein"
     gene            5476..7809
                     /locus_tag="VYN69_001531"
     CDS             5476..7809
                     /locus_tag="VYN69_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1533780.1"
                     /translation="MVEFIKSKKEMSEEDIKANFITPAIVSKGWKNGEHIAYEEYFTD
                     GRIEVRGDKARRKEGKKSDYSLYYQFGTRIAIVEAKDNKHSVRAGLQQAIEYGEILDV
                     PFVYSSNGDGFIEHDRITREERELELDEFPTREELFSRMTKEKGLTYEITEAISTPYY
                     TDAFSMKTPRYYQQIAINRTIETVARGQKRVIFVMATGTGKTFMAFQIIHRLRKAGLA
                     KRVLFLADRNILVDQTMAEDFKPFEKVMTKITPKLLTAPEKLNSFEIYLGLYQQLTGE
                     DGTETHYQKFDKDFFDLIVIDEAHRGSAKENSNWRKIIDYFSSATQIGMTATPKETKN
                     ASNTEYFGEPIYTYSLKQGIEDGFLAPYRVMRVNLDVDVDGYRPETGKVDANGQLIED
                     RYYSRKDFDKTIVIDDRTQRVAKFVSDYMKQNNARFDKTIVFCVDIDHAERMRAALVK
                     ENLDLVQEDYRYVMQVTGDNAEGKAQLDNFMDVNSNFPAIVTTSKLLTTGVNAKTCRL
                     IVLDSNIQSMTEFKQIIGRGTRLYPQKGKEFFTIIDFRNVTNLFADPDFDGEPVKVLE
                     TGAKTVSGSTPGFVDEEGDPVEKYIVTDKQVTILNSTVQVLDENGKLITESLTDYTRK
                     NILGSYATLNDFITVWHTADKKKLILDELYKKGVYLDAIRESEGISEQEIDDFDLLLK
                     LAYGQKELTKTERINKLKQSGYLYKYSEEARAVLEILLNKYMDKGIGELESIETLKLP
                     EFQIYGGTFKIINTYFGDKKRYLQAIKELEQELFTVA"
     gene            7822..9285
                     /locus_tag="VYN69_001532"
     CDS             7822..9285
                     /locus_tag="VYN69_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000029054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HEV1533781.1"
                     /translation="MSITSFVKRIQDITRNDAGVNGDAQRIEQMSWLLFLKIYDSREM
                     VWELEEDEYESIIPEELKWRNWAHAQNGERVLTGDELLDFVNNKLFKELKELEITSIM
                     PIRKTIVKSAFEDANNYMKNGVLLRQVINVIDEVDFNSPEDRHSFNDIYEKILKDIQN
                     AGNSGEFYTPRAATDFIAEVLNPKLGESMADLACGTGGFLTSTLNRLSSQRKTSEDTK
                     KYNTAVFGIEKKAFPHLLAVTNLFLHEIDDPKIVHGNTLEKNVREYTDDEKFDIIMMN
                     PPFGGSELETIKNNFPAELRSSETADLFMAVIMYRLKENGRVGVILPDGFLFGEGVKT
                     RLKQKLVDEFNLHTIIRLPHSVFAPYTGIHTNILFFDKTKKTEETWFYRLDMPDGYKN
                     FSKTKPMKSEHFNPVRDWWENREEILEGKFYKSKSFTPSELAELNYNLDQCGFPKEEE
                     EILNPFELIQNYQAERATLNHKIDNVLADILQLLEDK"
     gene            9285..>9809
                     /locus_tag="VYN69_001533"
     CDS             9285..>9809
                     /locus_tag="VYN69_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000187281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1533782.1"
                     /translation="MTPEQLKASILQRAMEGKLVPQNPNNEPASELLKRIKAEKEKLI
                     SEGKIKRDKKETEIFRGDDGKHYGKFADGSTQEIDVPYDIPDTWEWVRIKSIYWNFGQ
                     NKPEKSFRYIDTSSIDRKKNIINYKNLQYLSPEQAPSRARKLVSQNSVLFSTVRPYLK
                     NIAVVRELKEYLIAS"
BASE COUNT         3062 a         1828 c         1910 g         3009 t
ORIGIN      
        1 aaatcatacc aattatgaac ggtattacaa atttatatgt ttgtatttct aatgatatca
       61 tagctgatac cagcgccgat acaaatgcag acaacaatac tgaggtaaca aaattcctta
      121 ccgcttcatc atttttgata caactatcat gttcagctaa tataatttct ctatatctag
      181 ctaaatattc tattggaata ttatcaataa aatcttttga tgttctatag ttatcagctt
      241 gattacaaaa atcattataa acttttcgtg tattttttag atactcttca tccaatacac
      301 tcatcttact tttccgtaaa ctctccgtct acgacgtcat cgcctgcgtt ccctgttgct
      361 tgtgcgcctt ctgctccttc ttgagcttgt tgcgctgctg cggcttgttc gtagagttta
      421 acagcaaggc cttgagcttt ttcgttcaac gcttcaagtt tcgctttcat gtcgtccaag
      481 ttgttgtctt cttgagcttt cttaaggtca tcaagggcag cttgggcagc gtcacgttct
      541 gcgtcgaagc ctttaccttc agtttccttg attgtctttt cagtcgcaaa gattgcttgg
      601 tctacttcat tacgaaggtc tacttcttct ttacgtttct tatcggcttc agcgtttgct
      661 tctgcatctt tcatcatgcg gtcgatttct tcgtcagtca aacctgagtt cgattggatg
      721 acaatagttt gttctttttg agttccaagg tctttggcct taacagacac gataccgttc
      781 ttgtcgatgt caaatgttac ttcgatttga ggaattccac gaggtgcagc tgggatatca
      841 gtcaattgga agcgtccaag agtcttgtta tctgctgcca ttgggcgttc accttgaaga
      901 acgtggatat caacggctgg ttggttgtct gctgctgttg agaagacttg tgatttagat
      961 gttgggattg tagtgttgcg atcgataagt tttgtaaata ctccacccat tgtttcgata
     1021 ccaagtgaca atggcgttac atcaagaagg acaacgtcct tgacatcacc agtaatcaca
     1081 ccaccttgga tagccgcacc catagcaact acttcatcag ggtttactga tttgtttggt
     1141 tctttaccag tttcagcttt aacagcttca acaacggcag ggatacgagt tgaaccacca
     1201 acaaggataa cttcgtcgat ttctgacaag ctcaaacctg catctgaaag ggcttgacga
     1261 actggaactt ttgtacgttc aacaaggtca cgagtcaaat catcaaattt cgcacgagtc
     1321 aaagtcattt ccaagtgaag aggtccagcc tcacctgcag tgataaatgg caagctgatt
     1381 tgtgttgaag ttacaccaga aaggtctttc ttcgcttttt cagccgcatc tttcaaacgt
     1441 tgcattgcca tcttgtcagt agacaagtcg ataccgtttt ctttcttgaa ttctgctacc
     1501 aagtggtcaa tgattttttg gtcaaagtcg tcaccaccaa gtttgttgtc ccctgcagtt
     1561 gacaatacgt cgaagacacc gtcacccaat tcaaggatag agacgtcgaa tgtaccacca
     1621 ccaaggtcaa ataccaagat tttttcttct ttgtcagtct tgtccaaacc ataagcaaga
     1681 gctgctgcag ttggttcgtt aacaatacgt tctacttcaa gaccagcaat tttaccagcg
     1741 tcttttgttg cttgacgttg agcgtcgttg aagtaagccg gaactgtgat aacagctttg
     1801 gttactttct caccaaggta gtcttcagcg tagcctttca agtattgaag gatcatagct
     1861 gagatttctt gtggagtgta ttcttttcca tttgcagaaa ctttttcaga agttcccatc
     1921 ttagatttga tagagataac tgtatctggg tttgtaactg cttgacgttt tgcagcatca
     1981 ccaacgatga tttctccgtt tttgaatgag actacagatg gagttgtgcg gtttccttct
     2041 gggtttgcga tgattttgct ttcagttcct tcaagaactg caactgctga gtttgttgta
     2101 cctaagtcaa taccgataat tttagacatg tgtttttctc cttgttagtt aattttctat
     2161 ttttcttttt gatactcttc ttaagtggcg acgccgtcag agcttgactt cgtcaatctc
     2221 tttgactaaa ctttgagcct aaggtctcaa aatttgcgca aatagcacca cggcgaagag
     2281 tatcgtcttt ggttcgcttc gctcactatt acaaagccaa acatatttta tctttcttca
     2341 tagtttattg tcgtttcgga caagttttct attatgtaaa ttgcgacacg aggagtcgaa
     2401 atcgatttta tttcgacgac gagttagtaa ggaagctagg caaacgtcat agcgattgcc
     2461 gttttctgac gagttgcttt agctaccgtc agaattgccg aatcgaacac gccctaagct
     2521 ctgagtaaaa aagatagatt ttcttgtgtt catcgaacac ttcgtcaatc tcctattttt
     2581 actgcgagct ttttacgagc tttgtatctt agttatacac cactaccatt gctgggcgta
     2641 ggatgcggtc atggagtttg tagccttttt gaaagacttg ggcgatggta tctactgggt
     2701 gttcatcgtc tgctgggaga gtttggatgg ccatatggta gttatggtca aattcgccat
     2761 ctgctgcgat ttcttcaatt ccttcttctt tcaaagcgtg aatcaagctt tcttgcacca
     2821 tcgccaagcc cttcttcaca tcatctgtca aaccttcaac tgcaagtgca cgctcaaggt
     2881 tgtcaagaga tggtaagatt gcttttgcca agtcctggct acgataacgt tgcaagtttt
     2941 gacgttcttc attggcacgg cgttggatat tttgcatttc tgcatgagcg cgaagatatt
     3001 tgttttcgaa ctcatctgca cgttcatttg ccaagtccaa ctcagacttt tcaggagttg
     3061 tttcttcagc tgtttccaca acttcctctt cttgaacttc ttctacttct tcatttttta
     3121 tatcttgggc catgttcgcc tcctttaatg atttcaatct taatgtactt cgtaatgatt
     3181 actgctgagg tagcggtaaa aatctgtcaa cttcatggtc aaaacacggt tgaccacatt
     3241 gacttgattg attagctgtt ggtaatccag attaactgga ccgataatgg ctagaattcc
     3301 aactccccga taaggaatga ggaacttact actaatcacc gctaggtcag ctaaacagga
     3361 ctcttgaccg tctgcaacac gaacattttg catctgatcc tcacgcaacc cctcacgaat
     3421 ctccaaggcc accttttgcg gttggtcaaa gaactgatag gcttctagat tggcaaaatt
     3481 caagagatgg accttgcccg ccatcacaat gttttcgttg aacatttcct taaagatgtg
     3541 ttcaaagaga tcgatgacat tatccgttgt tgtaaagtaa cgctggataa tctgcggaat
     3601 ctccgtccga atcttgtagt gaatatctaa aacggtgtga ccgaggaaac gttcctgaat
     3661 gatgctcttc agtttcagca aatcctcctg caagaagttc cttggaatca gaaactgact
     3721 agtaaccgtt cgcgactcgt ctagggtaaa taccgccaag gctgtatgtt gccccaaaac
     3781 aacgatatca aaggctgtca aacgttgcct gctcggctca acatccagtg ccactaccgt
     3841 acagccactc aggtctgtta gcaagttagc agcctcttgc agaatatcct ccaatttgaa
     3901 gaattcctga tcaaaggctt tgacaatctc atatacctca ttttcagcca gccggtcaaa
     3961 atccagtgag tgtttcacat agtactgaaa accagcaaca cttggcatcc gaccgcttga
     4021 agtatgagcc ttctcaagca acccttgttt ttctagttcc gccatgtcat tacgaatggt
     4081 tgcactgcta gagttaatag actcttgcaa ggcttttgat ccgacaggtt cgtgcgtttt
     4141 ggtaaagatg tcaataatca gatttaaaat atcctgctga cgctctgtaa ccatctcatc
     4201 actcctctct gtctgatcaa ttagcactca atgcatccaa gtgctaactc atgattctat
     4261 tatacaccct tagaagagaa tgtcaagaca aaaactcaaa aaattagcac tcttttatca
     4321 agagtgctaa aaatcaatct gaagacctag aaccttgcta ttcatctact cggtatttcc
     4381 tttttagtct gaaataacca taaattagat aagaaatcag aaaagcatat aaaacaaaaa
     4441 taagaaagaa agacatagaa agatcctctc taaacaaact catacaaaca acaagaccac
     4501 ctgagaaagt agctgtacag gaacgaagtc tagatcgcat aacttcccat ttgagccctt
     4561 tactcatata gacttgatcc tctggaagta aatcagaaaa cgatttacga agaatcgaac
     4621 aagaacctgc tcctatcaat tcccaacctc tatctctaaa atcttgcagt tcatgcttat
     4681 acttttttaa gaaatctaga atcatagata cggtagatga catcgtctgg ttgacattgg
     4741 tcaaaataga acaaaccaaa acgactcgtt ctatacctcc aacctttcaa atgcatctca
     4801 tgtaaatgtt cttcttcctt gtccaaatca acaatggtga aaatccgaaa ttctactctg
     4861 ctattcattg tcttacccca aaattagaaa acatgcctgg cgttatttat tagataattc
     4921 tttccacttt tgactcaatc tccaaaaaat ataagaaatc tgaatcgcaa aaactatcaa
     4981 taaaacccaa tctattatga aaatcaaaaa cactttccaa ctgaaagaac tacctccagt
     5041 gacaaacttt gagaaaaacg gtagtagagc taaaaagaga aataaaatag gaagcatccg
     5101 cattgttaaa atccgtttgg cataaaaaaa tctttattta aacgaaaata ttatggcaaa
     5161 atttacgcca gtttttgaac ggctgatgta gatattttat actttcaaaa tgtttaaatg
     5221 tgattattta tttttgaaaa atagatcacc agcccgactg aaagtgctta tagaatgata
     5281 ataagtcgcc tgccgaaaac agcgaaaaat agcggtgtta tgcggagata atctgacgcg
     5341 atgcgaaagt atattgcata cttattttca acaatttagc agagtatttt tataagtgtg
     5401 atataataga agtataattt gttctgatag tttattttat ggagaagtag atttttagaa
     5461 tgcggagggt tcaatatggt tgagtttata aagtctaaga aagaaatgag tgaggaggat
     5521 attaaagcaa atttcatcac tcctgctatt gtatccaaag gatggaaaaa tggtgagcat
     5581 atcgcttacg aagaatactt cactgatggt cgaattgaag ttagaggaga taaggctcgt
     5641 cgtaaagaag gaaaaaaatc agactattca ctgtattacc aatttggaac tcgaattgca
     5701 attgttgagg caaaggataa taaacacagc gttcgagcag gattacaaca agctattgaa
     5761 tatggagaga ttttagatgt tccatttgtt tattcttcga atggtgatgg ctttattgaa
     5821 cacgaccgta tcacgagaga agaacgtgag ctggagttag acgaattccc tactcgtgaa
     5881 gaattatttt ctcgtatgac gaaggaaaaa ggattgacgt acgaaattac agaagctatc
     5941 tcaactccat actatacaga cgccttctca atgaaaacgc cacgctatta tcagcaaata
     6001 gctatcaacc gtactattga aacagttgcc agaggacaaa aacgagtaat atttgtgatg
     6061 gcaacaggaa cggggaaaac gttcatggct tttcaaatta ttcatcgcct tcgaaaagct
     6121 ggtttggcta aacgagtttt attcttagcg gatagaaaca tcttagtaga ccaaacgatg
     6181 gctgaagact ttaagccatt cgaaaaggta atgacgaaaa ttacaccaaa acttttgact
     6241 gctcctgaaa aattaaattc ttttgaaatt tatctagggc tttatcagca actaactggt
     6301 gaagatggaa ctgaaacaca ttatcaaaaa tttgacaaag acttctttga tttaatcgta
     6361 attgatgaag cgcaccgtgg ttcagctaag gaaaacagta actggcgtaa gataattgat
     6421 tatttcagtt ctgcgacaca gattgggatg accgctactc ctaaagaaac caagaatgct
     6481 tccaatacgg aatactttgg tgagccaatc tatacttata gtttaaaaca gggaatcgag
     6541 gatggttttt tggctccata tcgtgttatg agggttaatt tagatgtgga tgtggatggt
     6601 tatcgtccag aaactggaaa agttgatgct aacggacaat taatagaaga taggtactac
     6661 agcaggaaag attttgataa aaccattgtc attgatgata gaacgcaaag agttgccaag
     6721 tttgtttctg attatatgaa gcaaaacaat gcacgatttg ataaaacaat tgttttttgt
     6781 gttgatattg accatgccga gcgaatgcgt gctgcacttg taaaagagaa tctagactta
     6841 gtccaagaag actatcgtta tgtcatgcaa gtaactggtg acaacgctga aggaaaagct
     6901 caactggata actttatgga tgtcaattct aattttcccg ctattgtaac aacgtctaaa
     6961 ttattaacga caggagttaa tgctaaaaca tgtcgtttga ttgttttaga ctctaatatc
     7021 caatccatga ctgaatttaa acaaattatt ggtcgtggca cacgtcttta tcctcaaaag
     7081 gggaaagaat tttttacgat tattgatttt cgaaatgtta ccaatttgtt tgctgaccct
     7141 gattttgatg gtgagccagt gaaggtgctg gaaacaggtg cgaaaacagt cagtggttct
     7201 acgcccggtt tcgtagatga ggaaggtgac ccagtagaaa aatatatcgt tacagacaag
     7261 caggttacca ttcttaattc tactgttcaa gtattggatg aaaacgggaa actgattacc
     7321 gaaagcctga ccgactacac tcggaagaat atcttaggta gctacgccac tttgaacgat
     7381 tttatcacag tttggcatac ggcggataaa aagaagctta tcttagacga actttataaa
     7441 aaaggagttt atctagatgc tattcgagag tcggagggaa tatcagaaca agaaatcgat
     7501 gattttgatt tactcctaaa acttgcctat ggtcaaaaag aattaaccaa aacggaacgt
     7561 atcaataaac tcaaacaaag cggatattta tataaatata gtgaggaagc gcgtgctgtt
     7621 ttggaaattt tactgaacaa atacatggat aaaggtattg gagaactcga aagcattgaa
     7681 acattaaaac ttccagaatt tcagatatat ggtggaacct tcaaaatcat caatacttat
     7741 tttggagata aaaaacgata tttacaagca attaaagaat tggagcaaga gctatttaca
     7801 gtagcttaat gaaaggaaag tatgtcaatt acatcatttg taaaaagaat tcaagatatc
     7861 actcgaaacg atgctggtgt taatggtgat gctcaacgta ttgagcaaat gtcttggtta
     7921 ttattcttaa aaatttatga tagccgtgaa atggtttggg aattagaaga agacgagtat
     7981 gagtcaatta tcccagagga attaaaatgg cgaaattggg ctcatgctca aaatggggaa
     8041 cgggtattga caggcgatga attacttgat tttgtcaata acaagttatt caaagagttg
     8101 aaagagcttg aaataacttc aattatgcct attcgaaaaa cgattgttaa atcagctttt
     8161 gaagatgcga acaactatat gaaaaatggc gtcttgttac gccaagtcat caatgttatt
     8221 gatgaagttg atttcaatag ccctgaagat cgtcattcgt ttaatgatat ttacgaaaaa
     8281 attcttaaag atattcaaaa tgctgggaac tcaggagaat tttatacgcc acgtgcagcg
     8341 actgatttta ttgccgaagt tcttaaccca aaacttggag aatcaatggc agaccttgct
     8401 tgcggaacag gaggcttctt gacttcgact ctgaaccgtt taagtagtca acgtaaaact
     8461 agtgaagata ccaaaaaata taatacagct gtttttggta ttgaaaagaa agcatttcct
     8521 catcttttag cagttacaaa tctgtttctt cacgaaattg atgaccctaa aattgttcat
     8581 ggaaatactt tagagaaaaa tgttcgtgaa tatacggatg atgaaaaatt tgacattatt
     8641 atgatgaatc caccttttgg agggtcagaa ttagaaacaa taaaaaataa ctttccagca
     8701 gaattacgga gttctgaaac agctgattta tttatggctg tcattatgta tcgtttgaaa
     8761 gaaaatggtc gtgttggagt tattttacct gatggttttc tatttggtga aggtgtaaaa
     8821 actcgcttga aacaaaaact ggtagatgag ttcaacttgc atacgattat taggttgcct
     8881 catagtgtct ttgcaccgta tacaggaatc catacgaaca ttcttttctt tgataaaaca
     8941 aagaaaacag aagaaacttg gttttatcgt ttagatatgc cagatggtta taaaaatttc
     9001 tcgaaaacta agccgatgaa gtcagaacac ttcaatcctg ttcgtgactg gtgggaaaat
     9061 cgtgaagaga ttctggaagg taagttctac aaatctaaat catttacacc tagtgaattg
     9121 gctgagttga attataattt agaccagtgt ggttttccaa aagaggaaga ggaaatctta
     9181 aatccctttg agttgattca gaattatcaa gcggaaagag caactttaaa tcataagatt
     9241 gataatgtat tagctgatat tttgcagttg ttggaggaca aataatgaca ccagaacaac
     9301 ttaaggcaag tattctccaa agagcgatgg aagggaaatt agtgccgcaa aatcccaata
     9361 acgaacctgc aagtgaatta ttaaagagaa ttaaagctga aaaagaaaaa cttatcagtg
     9421 aaggaaaaat caaacgagat aaaaaggaaa ctgagatatt tcgtggtgat gatgggaaac
     9481 attatgggaa gtttgctgat ggaagcactc aagaaattga tgttccttat gatattcctg
     9541 atacttggga gtgggtgagg ataaaatcaa tttattggaa ttttgggcaa aataagccag
     9601 agaaatcctt taggtatata gatacgtcta gtattgatag aaaaaagaac ataatcaact
     9661 acaaaaatct acaatatctt tcacctgaac aagcgccttc ccgtgctaga aaattagttt
     9721 cgcagaatag tgtcttattt tcaacagtta gaccatatct aaaaaatatt gctgtagtta
     9781 gagaacttaa agagtatttg atagctagt
//
LOCUS       DAVLVP010000067         9804 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.067, whole genome shotgun sequence.
ACCESSION   DAVLVP010000067 DAVLVP010000000
VERSION     DAVLVP010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9804)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9804)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9804
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.067"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            278..370
                     /locus_tag="VYN69_001534"
                     /pseudo
     CDS             278..370
                     /locus_tag="VYN69_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(626..1246)
                     /locus_tag="VYN69_001535"
     CDS             complement(626..1246)
                     /locus_tag="VYN69_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000780815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1533783.1"
                     /translation="MKLYFVRHGRTLWNQEGRFQGASGDSPLLPESIETLKRLGQYLK
                     EIPFDQIYSSDLPRAVKSAEIIQSQLHTPCSLEIVPNLREWQLGKLEGLKIATLEAIY
                     PQQIQAFRSNLAQFDTRMFGAESLYSTTQRTIQFIKSLKDSPADRILIVGHGANLTAS
                     LRTLLGYKEPLLRKDGGLANASLTIIETHDFETFTLNTWNDTSYQS"
     gene            complement(1246..1728)
                     /locus_tag="VYN69_001536"
     CDS             complement(1246..1728)
                     /locus_tag="VYN69_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEV1533784.1"
                     /translation="MAKKVKIKKTLVEQILSKAAIPHQGIQINALEGELPQGYERDQI
                     FKTLALLGDKTGPIIGIVPITQHLSEKKLAKISGNKKVSMIPQKDLEKTTGYIHGANN
                     PVGIRQKHNYPIFIDKIALDLDRMIVSAGEVGHSIIVAPQDLASFVKADFVDILEDIK
                     "
     gene            complement(1728..2570)
                     /locus_tag="VYN69_001537"
     CDS             complement(1728..2570)
                     /locus_tag="VYN69_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004256605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEV1533785.1"
                     /translation="MLSWLARVIKGIVIALGFILPGISGGVLAAILGIYERMIGFLAH
                     PFKDFKENVLYFIPVAIGMLLGIGLFSYPIEYLLENYQVFVLWSFAGAIIGTVPSLLK
                     ESTRESDRDKIDLAWLWTTFIISGLGLYALNFVVGTLSASFLNFVLAGALLALGVLVP
                     GLSPSNLLLILGLYAPMLTGFKTFDFLGTFFPIGIGAGATLIVFSKLMDYALNNYHSR
                     VYHFIIGIVLSSTLLILIPNAGNAESIQYTGLSLVGYVIIAFFFALGIWLGIWMSQLE
                     DKYK"
     gene            complement(2665..3465)
                     /locus_tag="VYN69_001538"
     CDS             complement(2665..3465)
                     /locus_tag="VYN69_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HEV1533786.1"
                     /translation="MRKQIHPLILFSFFGIMMFILIINRPLNDSQSFKTKSNIAQIEA
                     QALKHLDKPIIDLSGWQRPEEINYDALSQNISGAIVRVHSGAQTTKENDASFINGIDK
                     AYKSHITELQKRNVPVAVYAYVAGKSVQEMEKAAEVFYNAASPYSPSYYWLDVEDKTM
                     SNMNEGVENFRAKLASLGAKNIGIYVGVYFMEEHSIDTGKFTSVWIPSYGSDSGFLES
                     SPKTDLDYDIHQYTSKGKIAGFDHDLDINVISPLKNKEETFRKLFLKP"
     gene            3609..4988
                     /gene="glmU"
                     /locus_tag="VYN69_001539"
     CDS             3609..4988
                     /gene="glmU"
                     /locus_tag="VYN69_001539"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEV1533787.1"
                     /translation="MSNFAIILAAGKGTRMKSDLPKVLHKVAGISMLEHVFRSVGAIQ
                     PEKTVTVVGHKAELVEEVLAGQTEFVTQSEQLGTGHAVMMTEPILEGLSGHTLVIAGD
                     TPLITGESLKNLIDFHINHKNVATILTAETDNPFGYGRIVRNDNAEVLRIVEQKDATD
                     FEKQIKEINTGTYVFDNERLFEALKNINTNNAQGEYYITDVIGIFRETGEKVGAYTLK
                     DFDESLGVNDRVALATAESVMRRRINHKHMVNGVSFVNPEATYIDIDVEIALEVQIEA
                     NVTLKGQTKIGAETVLTNGTYVVDSTIGAGAVITNSMIEESSVADGVTVGPYAHIRPN
                     SSLGAQVHIGNFVEVKGSSIGENTKAGHLTYIGNCEVGSNVNFGAGTITVNYDGKNKY
                     KTVIGDNVFVGSNSTIIAPVELGDNSLVGAGSTITKDVPADAIAIGRGRQINKDEYAT
                     RLPHHPKNQ"
     gene            4998..5543
                     /locus_tag="VYN69_001540"
     CDS             4998..5543
                     /locus_tag="VYN69_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1533788.1"
                     /translation="MEFEEKTLSRKEIYQGPIFKLVQDQVELPEGKGTAQRDLIFHNG
                     AVCVLAVTDEQKLILVKQYRKAIEAVSYEIPAGKLEVGENTAPVAAALRELEEETAYT
                     GKLELLYDFYSAIGFCNEKLKLYLASDLTKVENPRPQDEDETLEVLEVSLEEAKELIQ
                     SGHICDAKTIMAVQYWELQKK"
     gene            5555..5869
                     /locus_tag="VYN69_001541"
     CDS             5555..5869
                     /locus_tag="VYN69_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HEV1533789.1"
                     /translation="MGKSLLTDEMIERANRGEKISGPPLLDDNEETKILPTSSSRFGY
                     ANPKDHGFSQETLKIQVEPSIHKSRRIENTKRNVFNSKLNKILFAVIFLLILLVLAMK
                     LL"
     gene            5886..6578
                     /locus_tag="VYN69_001542"
     CDS             5886..6578
                     /locus_tag="VYN69_001542"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEV1533790.1"
                     /translation="MKIGIIAAMPEELAYLVQHLDNAQEQVVLGNTYHTGTIASHEVV
                     LVESGIGKVMSAMSVAILADHFQVDALINTGSAGAVAEGIAVGDVVIADKLAYHDVDV
                     TAFGYAYGQMAQQPLYFESDKTFVAQIQESLSQLDQNWHLGLIATGDSFVAGNDKIEA
                     IKSHFPEVLAVEMEGAAIAQAAHALNLPVLVIRAMSDNANHEANIFFDEFIIEAGRRS
                     AQVLLAFLKALD"
     gene            6655..7146
                     /gene="rocS"
                     /locus_tag="VYN69_001543"
     CDS             6655..7146
                     /gene="rocS"
                     /locus_tag="VYN69_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein RocS"
                     /protein_id="HEV1533791.1"
                     /translation="MSIEMTVSEIAEVLGLSRQAINNRVKELPEEDTDKNDKGVTVVT
                     RSGLIKLEEIYKKTIFEDEPVSEDVKQRELMEILVDEKNAEILRLYEQLKAKDRQLSE
                     KDEQMRIKDRQIAEKDKQLDQQQQLTLQAMKDQENLKLELDQAKEEVQSTKKGFFARL
                     FGG"
     gene            7244..7834
                     /locus_tag="VYN69_001544"
     CDS             7244..7834
                     /locus_tag="VYN69_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HEV1533792.1"
                     /translation="MERLEDYIAFDLEFNQHEGQTHLIQVSAVRFRDGREIDAYDSYV
                     HTSVPLKSFINGLTGITAETLKDAPPVEKVLEEFQEFVGSLLMIGYNAAKSDLPILAE
                     HGLDYSQQYKVDLYDEAFERRSSDLHGIANLKLQTVASFLGFQGQSHNSLEDARMTAR
                     VYESFLESDQARELLETESSLSNNPFGGLDLSQLFD"
     gene            7843..9606
                     /locus_tag="VYN69_001545"
     CDS             7843..9606
                     /locus_tag="VYN69_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEV1533793.1"
                     /translation="MKPEKPKKLGLKQIYLNLDKILFLFFLIFMIVEYLWVPFNSWLA
                     SFLLDQTGYLFISYNNVLAILTHSPLISLAFLALIVINLLVAYFQIGLLFIGARHLLY
                     NEKRRLIEYIRKVFHESFGFMKKLTCAKALFIFFYIAMLFPFIRKMLKIYYLNKIVIP
                     EFIQAYLEDRYWMWWLSILLLSLIFLYVSVRLMFALPKIVYDQLTVREAVMFSLEKTK
                     KRVTFYAWNLFYILLKAHLLFYLPLIPLLLAQTLVDDITQRESLILGILNFVVIKNLY
                     YMALTYFLVKFVSFLTGKELDMLPRREKDHIVRWGVMTCASLFFALEGYIYLEAPMVH
                     LPQLISHRGVSNANGIQNTVESLETTAQLKPDLVETDVQETKDGQFVMMHDANLKNLA
                     GINKSPQDLNLEELKGIDIFENGYQTKISSFEDYLSRANELGQKLLIEIKTSKKDSPD
                     MMNRFLARYAAMLKIYGHQIQSLDYHVVEKVRQYDAELPVYFIMPYNSVFPKTRATGY
                     TMEYSTLDEYFVSKLWTTDQKLYVWTVNDSEAISKSLHLGVDGVITDDLEKVQREIEV
                     AQEDPEYTDLLLKKALEFFEF"
BASE COUNT         3034 a         1700 c         2086 g         2984 t
ORIGIN      
        1 taaaaacacc ccaaaagtta gattttttct gtctaacttt tggggggcag ttcatttaac
       61 acctgataat atgcgttttt ttattttaaa gactttttga ccagcctcat ttttttaact
      121 tgatactcag tgaaaagcaa aaattaaact aggaagctag ctgtaggctg ctcaaagaac
      181 agctttgagg ttgcagataa aacttgtgag gtcaccaaca tatataatgt gaagctgaca
      241 tggtttgaat agattttaga agggtatgag tctggaactt ttaatggata atgcaagatt
      301 ccatagaatg ggtaagctag agttcttatg tgaagagttt gggcataaac ttttaccttt
      361 tcctccctac ttatcttagt atagaaaagt gaatctgaaa tagtacataa ctgcttctaa
      421 aacattctta taatatgatt taaattctca aatcatatta ttcagttctt atttcatttt
      481 gctctacaat cctgttgaga agacacgtgt tcatatcaaa aaggtattgg caagttgcaa
      541 taccttttta cgaggctctt ttatcttatt tttgtttcaa ctgactatat ctcctatggt
      601 tctagttcag aaggctaggc tataattatg attgataaga agtatcattc caagtattga
      661 gagtgaatgt ttcaaaatca tgggtttcta taatggtcag gctggcattt gctagaccgc
      721 catctttacg aagaagtggt tctttatagc ctaggagagt acgaagactg gcagtaagat
      781 tggcgccgtg tccgacaatt agaatacgat cagctggact atcttttaat gatttgataa
      841 attggatggt ccgctgagtt gtactataga gggattcggc tccgaacatt cgagtgtcaa
      901 attgagcaag atttgaacga aaagcctgga tttgttgcgg gtaaatagct tccaaggttg
      961 caattttcaa accttctaac ttcccaagtt gccattcacg gagattagga acgatttcta
     1021 aagaacaggg ggtatggagt tgactttgga taatctcagc agatttgacc gctcgaggta
     1081 aatcacttga ataaatctga tcaaaaggaa tttccttgag atactggcca agtcgtttta
     1141 gggtttcaat ggattcagga agaagaggag aatcaccgct agcaccttga aaacgacctt
     1201 cttggttcca gagggtacga ccgtggcgga caaagtagag tttcattact tgatgtcctc
     1261 caaaatatct acaaagtctg cctttacaaa gctagccaag tcttgtggcg cgacgataat
     1321 gctgtgtccg acttcgcctg cagagacaat cattcgatcc aaatctagag caattttatc
     1381 gataaaaatg ggataattgt gtttctgacg aattccgaca ggattattgg ctccatgaat
     1441 gtaaccagtt gttttttcta agtccttttg tggaatcatg ctcacttttt tattgccaga
     1501 aattttagct agtttctttt cagacaagtg ctgagtgata gggacaattc cgataatcgg
     1561 tccggtcttg tctcccaaaa gcgccaaggt tttgaaaatc tgatctcgtt cataaccttg
     1621 aggaagctct ccttctaggg cattgatttg aatcccctga tgagggatag ctgctttaga
     1681 taggatttgt tctaccaatg tttttttgat tttaactttt tttgccatta tttatattta
     1741 tcctccaatt gactcatcca aataccaagc cagattccca gcgcaaagaa gaaggcgatg
     1801 atgacataac cgacaagtga aagtcctgtg tattggatac tttcagcgtt tcctgcattt
     1861 ggaattaaga tcaaaagggt acttgatagg acgataccga tgatgaaatg atagacgcgt
     1921 gagtggtagt tgtttaaggc ataatccatc aattttgaaa aaacgatgag agttgcacct
     1981 gcaccaattc caatcggaaa gaaggttccc aagaaatcaa aagttttaaa accagtcaac
     2041 ataggagcat agagtcccaa aatcaaaagt aaatttgatg ggctgaggcc aggaaccaag
     2101 acgccaaggg ccaatagtgc gcctgctagg acgaagttaa gaaagctggc gcttaaggtt
     2161 ccaacgacaa aatttaaggc atagagtcct aatccagaaa tgataaaggt tgtccataac
     2221 caagctaaat caatcttgtc tcggtcagat tctcgagttg attctttgag gaggctagga
     2281 actgtaccga taatagctcc cgcaaagctc cataatacaa aaacctgata attttcaagc
     2341 aggtattcaa tcgggtagga aaataagccg attcccagaa gcataccgat ggcaactgga
     2401 ataaagtaca aaacattttc tttaaagtct ttaaagggat gggccagaaa gccaatcatt
     2461 cgttcataga ttcctaagat tgctgctaga acccctccgg aaattcccgg taggataaat
     2521 ccaagagcaa ttacaatccc tttaataacg cgtgctaacc atgagagcat atatttcctc
     2581 caactatttc tatttcaaaa aatccttact atattgtatc acaatcagac acaaaaagaa
     2641 aaagcaaatg ataaacaaat gcttttaagg ttttaaaaaa agctttcgaa aggtttcttc
     2701 tttatttttt aagggagaga taacgttgat atctaaatcg tggtcaaagc cggcaatttt
     2761 tcctttagat gtgtattggt gaatatcata atctaaatca gttttaggac tgctctccaa
     2821 aaatcctgag tctgagccgt aggacggaat ccaaacagag gtaaacttgc ctgtatcaat
     2881 actgtgttct tccatgaagt agacaccaac gtagatgccg atgtttttag cacctagtga
     2941 tgctagtttt gctcgaaagt tttcgacacc ttcgttcata ttagacatgg ttttgtcttc
     3001 cacgtcaagc caatagtaac tagggctgta aggagaggca gcattgtaga aaacttcggc
     3061 agccttttcc atttcttgga cactttttcc agctacataa gcgtagacag caactgggac
     3121 attccgcttt tgaagttcag tgatatgact cttataggcc ttgtctattc cattgataaa
     3181 tgaagcatca ttttcttttg ttgtttgagc accactgtga acacgaacaa tagcacctga
     3241 aatattttgt gagagggcat cgtagttgat ttcctcagga cgctgccagc cagagaggtc
     3301 aataatcggt ttgtctaagt gtttcaaagc ctgtgcttca atctgtgcta tattggattt
     3361 tgttttaaac gattggctgt cattaagtgg gcgattgatg attaaaatga acatcataat
     3421 cccaaaaaaa ctaaataaaa taagtggatg aatttgtttt ctcatatctt ataagtctac
     3481 cctaaaaatc aaaaaaaatc aaaaaaatgg gttaaggaag agactttaga gcattttttc
     3541 attcaagagt gcggaatgat ttgaaatatg gtataataaa agggaatttc tacagaaaag
     3601 agaagattat gtcaaatttt gccattattt tagcagcggg taaagggact cgcatgaaat
     3661 ctgatttgcc aaaagttttg cacaaggttg cgggtatttc tatgttggaa catgttttcc
     3721 gtagtgtggg agctatccaa cctgaaaaga cagtaacagt tgtaggacac aaggcagaat
     3781 tggttgagga ggtcttggct ggacagacag aatttgtgac tcaatctgaa cagttgggaa
     3841 ctggtcatgc agttatgatg acagaaccta tcttagaagg tttgtcagga cacaccttgg
     3901 tcattgcagg agatactcct ttaatcactg gtgaaagctt gaaaaacttg attgatttcc
     3961 atatcaatca taaaaatgtg gccactatct tgactgctga aacggataat ccttttggct
     4021 atggacgaat tgttcgtaat gacaatgctg aggttcttcg tattgttgag cagaaggatg
     4081 ctacagattt tgaaaagcaa atcaaggaaa tcaacactgg aacatacgtc tttgacaacg
     4141 agcgtttgtt tgaggctttg aaaaatatca ataccaataa cgctcaaggc gaatactata
     4201 ttacagacgt cattggtatt ttccgtgaaa ctggtgaaaa agttggcgct tatactttga
     4261 aagattttga tgaaagtctt ggggtaaatg accgtgtggc gcttgcgaca gctgagtcag
     4321 ttatgcgtcg tcgcatcaat cataaacaca tggtcaatgg tgttagcttt gtcaatccag
     4381 aagcaactta tatcgatatt gatgttgaga ttgctctgga agttcaaatc gaagccaatg
     4441 ttaccttgaa agggcaaacg aaaattggtg ctgagactgt tttgacaaac ggtacttatg
     4501 tagtggacag cactatcgga gcaggagcgg tcattaccaa ttctatgatt gaggaaagta
     4561 gtgttgcaga cggtgtgaca gtcggtcctt atgctcacat tcgtccaaat tcaagtctgg
     4621 gtgcccaagt tcatattggt aactttgttg aggtgaaagg atcttcaatc ggtgagaata
     4681 ccaaggctgg tcatttgact tatatcggaa actgtgaagt gggaagcaac gttaatttcg
     4741 gtgctggaac tattacagtc aactatgacg gcaaaaacaa atacaagaca gtcattggag
     4801 acaatgtctt tgttggttca aattcaacca ttattgcacc agtagaactt ggtgacaatt
     4861 ccctcgttgg tgctggttca actattacta aagacgtgcc agcagatgct attgctattg
     4921 gtcgcggtcg tcagatcaat aaagacgaat atgcaacacg tcttcctcat catcctaaga
     4981 accagtagga gcctatcatg gagtttgaag aaaaaacgct tagccgaaaa gaaatctatc
     5041 aaggaccaat atttaaactg gtccaagatc aggttgaatt accagaaggc aagggaactg
     5101 cccaacggga tttgattttt cacaatgggg ctgtctgtgt tttagcagta acggatgaac
     5161 aaaaacttat cttggtcaag cagtaccgca aagctatcga ggctgtctct tacgaaattc
     5221 cagccggaaa attggaagta ggagaaaaca cagcccctgt ggcagctgcc cttcgtgaat
     5281 tagaggaaga aacagcctat acagggaaat tagaactctt gtacgatttt tattcagcta
     5341 ttggcttttg taatgagaag ttaaaactat atttagcaag cgatttgaca aaagtggaaa
     5401 atccgcgtcc gcaggatgag gatgaaacct tggaagtcct tgaagtgagc ttagaagaag
     5461 cgaaagaatt aatccaatca ggtcatatct gtgatgccaa gacaattatg gctgttcagt
     5521 attgggagtt gcagaaaaaa tagaggaggt cagtatgggt aaatctttat taacggatga
     5581 aatgattgaa agagctaata gaggcgaaaa aatttcaggt cctcctttgc tggatgataa
     5641 tgaggaaact aagattttac caacctcttc ttcccgtttt ggttatgcca atcctaagga
     5701 tcatggtttt agccaggaaa ccttgaagat tcaggtcgaa ccatctattc ataaaagccg
     5761 tcgtattgaa aataccaaga gaaatgtctt caattctaag ttgaataaaa tcttattcgc
     5821 ggtcatcttt ctcttgattt tgcttgtttt agcaatgaaa cttttgtaat agaaaaggaa
     5881 ttgaaatgaa aataggaatt attgctgcta tgccagaaga actggcttat ctggtccagc
     5941 atttagataa tgcccaggag caagttgttt tggggaatac ctatcataca ggaaccattg
     6001 cttctcatga agtcgttctt gtagaaagtg gaattggtaa ggtcatgtct gctatgagtg
     6061 tggcgatttt ggctgatcat ttccaggtgg atgcccttat taatacgggt tcagctgggg
     6121 cagtagcaga aggtatcgct gttggggatg tcgtgattgc tgacaaatta gcctatcatg
     6181 acgtggatgt cacagctttt ggctatgctt atggacaaat ggcgcaacaa ccgctttatt
     6241 tcgaatcaga caaaaccttt gttgctcaaa tccaagagag tttatctcaa ttggaccaaa
     6301 actggcatct tggtttgatt gctacaggag atagttttgt tgcaggaaat gacaagatag
     6361 aagcgattaa gtcccatttc ccagaagttt tagccgtgga gatggagggg gcagctattg
     6421 ctcaagcagc gcatgccctc aatctcccag tcttagtcat ccgagctatg agtgacaatg
     6481 ccaaccatga agcaaacatc ttttttgatg agtttattat cgaagctgga cgtcgctctg
     6541 cccaagtctt gttggccttt ttgaaggctt tagattaagc ggaaatttga cagtttttct
     6601 agcttatgat aagatttaag taaagaaaag ctagaaaacg tttcagagga tattatgagt
     6661 attgaaatga ccgtcagtga gattgcagag gtcttaggat tatctcgcca agcaatcaat
     6721 aaccgtgtca aagaattacc agaagaagac acagataaaa atgacaaagg ggtaacagtt
     6781 gttaccagaa gtggcttgat taagctagaa gaaatctata aaaaaacgat ttttgaagat
     6841 gagcctgtca gtgaagatgt caaacaacgt gaactgatgg agattctagt ggatgagaag
     6901 aatgcagaaa ttcttcgtct ttatgaacaa ttaaaagcca aggatcgtca gttatcagaa
     6961 aaagacgagc agatgcgtat caaagaccgt cagattgctg agaaagacaa acaattagat
     7021 cagcaacaac aattgaccct tcaagccatg aaggatcaag aaaatcttaa gctagagctg
     7081 gaccaagcaa aagaagaagt ccaatccact aaaaaaggct tttttgctcg tttatttgga
     7141 ggataatcaa ggagctgttt aggttaaatg ctaacctgat ggcttctttt gtttgtgaat
     7201 agtatagttg attagaacag agacagtaga ggagaggtac agaatggaac gattagaaga
     7261 ttacattgct tttgatttag aatttaatca gcacgaagga caaacacact tgattcaagt
     7321 atcggcagtg cgttttagag atggtcggga aatcgatgcc tatgattcct atgttcatac
     7381 cagcgtccct ctaaaaagct ttatcaatgg tttgaccggg attacagctg agaccttaaa
     7441 agatgctcct ccagttgaga aagtcttaga agaattccaa gagtttgtgg gttctttgct
     7501 gatgataggc tacaatgctg ctaaaagtga tttacctatt ttagcagagc atggtttaga
     7561 ctatagccaa cagtacaagg tggatttgta tgatgaagct tttgaacgtc ggagttcaga
     7621 tttacatggt attgccaatc tcaaattgca aactgttgcc tcatttcttg gatttcaagg
     7681 tcagtctcat aatagtttag aggatgctcg gatgacggcg cgtgtttacg aatccttctt
     7741 agagtcggat caagctaggg aattgttaga gacagaaagt agcttatcaa acaatccttt
     7801 tggtggcttg gacttgtctc aattatttga ctaggagtgc ctatgaaacc tgaaaaacct
     7861 aaaaaattgg gtcttaaaca gatttacctc aatcttgata aaatcctctt tctatttttc
     7921 ttgattttta tgatagtgga gtatttgtgg gtaccattca attcatggtt ggctagcttc
     7981 ttattagatc agacaggtta tctttttatt tcctataaca atgtcttagc catccttact
     8041 cattctccat tgattagtct agctttttta gccttgattg taattaacct tctggttgct
     8101 tattttcaaa taggactcct ctttattggt gctcgtcatc tcctctataa tgaaaagcga
     8161 aggctaattg agtatatccg caaggttttc catgaaagtt ttggatttat gaaaaagcta
     8221 acttgcgcta aagccttgtt tatctttttc tatatagcta tgctgtttcc ttttatacgg
     8281 aaaatgctga agatttatta tcttaataaa atcgtcattc cagagtttat ccaagcttat
     8341 ttagaagata gatattggat gtggtggctg agcatcctac ttctatctct tatctttctc
     8401 tatgtctctg tcagattgat gtttgccctt ccgaagattg tatatgatca gctgactgtt
     8461 cgagaagcag tgatgtttag cttggagaaa accaagaaga gagttacttt ttatgcttgg
     8521 aatttatttt acatcttgct caaagcacat ttattatttt atcttccttt gattccttta
     8581 ttattggctc aaactttggt agatgatatc actcagagag agtctctgat tcttggtatt
     8641 ttgaattttg tcgtgattaa aaatctctat tatatggctc tgacttattt tttggttaaa
     8701 tttgtttcat ttttgacagg aaaggagctg gatatgcttc ctaggagaga aaaagatcac
     8761 atcgtgcgat ggggtgtcat gacttgtgcc agtcttttct ttgccttaga aggttatatt
     8821 tatctggagg ctcccatggt tcatctacct caacttattt ctcaccgagg ggtttccaat
     8881 gcaaatggga ttcaaaatac agtagagtcc ttggaaacta cagcacaact caaaccagac
     8941 ttggtggaga cggacgtgca ggaaacaaaa gatgggcagt ttgtcatgat gcatgatgct
     9001 aacttgaaaa atctagcagg tatcaataaa agtcctcaag acttaaactt ggaggagctt
     9061 aaagggattg atatttttga aaatggctac cagactaaaa tttcaagctt tgaagattat
     9121 ctcagtcgag ccaacgaact tggtcaaaaa ttactaattg aaattaaaac cagtaaaaaa
     9181 gatagtccag acatgatgaa ccgcttttta gcccgttatg ctgcaatgct caagatttat
     9241 ggacatcaaa tccagtcttt agactaccat gttgtcgaaa aagtaagaca gtatgatgcc
     9301 gaactgccag tttatttcat catgccctac aattctgtct ttcctaaaac aagagcgaca
     9361 ggatatacta tggagtactc aaccttggat gaatattttg taagcaaact atggacaacg
     9421 gatcagaaac tttatgtctg gactgtcaat gattcagaag ctatcagcaa atctcttcac
     9481 ttaggagtag atggagtgat tacagatgat ttggaaaaag ttcagcggga aatagaagta
     9541 gcccaagaag atccagaata tacggatttg ctcttgaaaa aagcattgga attctttgaa
     9601 ttttagtaat aaaaaagagg aacggttgtt cctcccatga taggtaagta ttcttgtgaa
     9661 tgcttgcttt tttgtggttg caatccgttt acgagaattg tttgtattgt tcttgtttca
     9721 atttacttta tagtgctttg aagttggaat gctacactat aacttataaa atatagtaaa
     9781 atgaaccaaa aatagtacac aatg
//
LOCUS       DAVLVP010000068         9415 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.068, whole genome shotgun sequence.
ACCESSION   DAVLVP010000068 DAVLVP010000000
VERSION     DAVLVP010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.068"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(112..1308)
                     /gene="tuf"
                     /locus_tag="VYN69_001546"
     CDS             complement(112..1308)
                     /gene="tuf"
                     /locus_tag="VYN69_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014335040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEV1533794.1"
                     /translation="MAKEKYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLARRLPSSV
                     NQPKDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQM
                     DGAILVVASTDGPMPQTREHILLSRQVGVKHLIVFMNKVDLVDDEELLELVEMEIRDL
                     LSEYDFPGDDLPVIQGSALKALEGDSKYEDIVMELMNTVDEYIPEPERDTDKPLLLPV
                     EDVFSITGRGTVASGRIDRGIVKVNDEIEIVGIKEETQKAVVTGVEMFRKQLDEGLAG
                     DNVGVLLRGVQRDEIERGQVIAKPGSINPHTKFKGEVYILTKEEGGRHTPFFNNYRPQ
                     FYFRTTDVTGSIELPAGTEMVMPGDNVTIDVELIHPIAVEQGTTFSIREGGRTVGSGM
                     VTEIEA"
     gene            complement(2219..3088)
                     /locus_tag="VYN69_001547"
     CDS             complement(2219..3088)
                     /locus_tag="VYN69_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000348128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEV1533795.1"
                     /translation="MDFTWALKYATEFLGTAILIILGNGAVANVELKGTKGHQSGWIV
                     IAVGYGMGVMIPALMFGNVSGNHINPAFTLGLAVSGLFPWAQVVPYIIAQVLGAIFGQ
                     ALVVATYRPFYLKTENPNNILGTFSTISSIDHGTKESRYAATVNGLINEFVGSFVLFF
                     AALGLTKNFFGAEVLQFMKQKAIEAGQTVDFSDLAIKAQVAPHTASGLSVAHLALGFL
                     VMALVTSLGGPTGPALNPARDLGPRLLHAFLPKSVLGEHKGDSKWWYSWVPVVAPIAA
                     AIAAVAVFKFLYL"
     gene            complement(3311..3832)
                     /locus_tag="VYN69_001548"
     CDS             complement(3311..3832)
                     /locus_tag="VYN69_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1533796.1"
                     /translation="MVPKTATSTETKTITRIIHYVDKVTNQNVKEDVVQPVTLSRTKT
                     ENKITGVVTYGEWTTGNWDEVISGKIDKYKDPDIPTVESQEVTSDSSDKEITVRYDRL
                     STPDKPTPEPGTPKTETPVNPDPEVPTYETGKREELPNTGTEANATLASAGIMTLLAG
                     LGLGFFKKKEDEK"
     gene            complement(3790..4896)
                     /locus_tag="VYN69_001549"
     CDS             complement(3790..4896)
                     /locus_tag="VYN69_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEV1533797.1"
                     /translation="MNIILIAKLLRENTNTKANALNNGWARSGSEEFKKFSHFVGVDK
                     GIVRTNVLTGKKLSDKIRKEVGSGDSKLGKGGYFSTGDVLLGKDVVSYTVQVFSENNE
                     RVGVNTQSHRVQYNLPILADFSVIQDTVEPSRTVVEKIIPKLNIPEEEKGKITEEIKK
                     KKKTSELAELISENVKVRYVDEQGRLLSLKNDTGIGEKESDGTYITNKKQLIGTSYNV
                     TDKKLSSMTTTDGKYYTFKEADTNSASLTGNIVSEGRTVTLVYRESEAPTTATVTANY
                     YKEGSQEKLAESVIKADLAIGFEYTTESKTIEGKTTTEDKEDRVITRKTTYTLVATPA
                     NAYQKTVQQLTITTVRMLRKQWFPKQQPLLRRRL"
     gene            complement(5007..5261)
                     /locus_tag="VYN69_001550"
     CDS             complement(5007..5261)
                     /locus_tag="VYN69_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001842111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533798.1"
                     /translation="MLNVDQDFMSISKSNKSGSDWKKTFTVRITNRLANDLNNVLKQA
                     DKDTPNTPTWLNSAASKAKDDDRVYKLLKTLIPGENYLSC"
     gene            complement(5619..6065)
                     /locus_tag="VYN69_001551"
     CDS             complement(5619..6065)
                     /locus_tag="VYN69_001551"
                     /inference="COORDINATES: protein motif:HMM:NF024751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1533799.1"
                     /translation="MDETGIDRYLYRPYAGAPRGEKVYEKISGRRFERTSIVAGQVDG
                     EFIAPMIYKKSMTSDFFVEWFKTQLLPALKTPHVIVMGNAGFHPKNILDELCIQDKHF
                     FLPLPPYSPDLNPIEQAWAILKKKVTDVLREVPTIFECLECFFKTR"
     gene            complement(6138..6463)
                     /locus_tag="VYN69_001552"
                     /pseudo
     CDS             complement(6138..6463)
                     /locus_tag="VYN69_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331855.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            6697..8415
                     /locus_tag="VYN69_001553"
     CDS             6697..8415
                     /locus_tag="VYN69_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004252698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEV1533800.1"
                     /translation="MSYQENYQKWVDFVELPDYLRQDLENMDEKTKEDAFYTNLEFGT
                     AGMRGLVGAGTNRINIYVVRQATEGLARLIESKGGNEKERGVAIAYDSRHFSPEFAFE
                     SAAVLAKHGIKSYVFESLRPTPELSFAVRHLNCFAGIMVTASHNPAPFNGYKVYGEDG
                     GQIPPHDADALTTYIRAIENPFAVEVADVETEKASGLIEVIGEAVDVEYLKEVKDVNI
                     NPALIEEFGKDMKIVYTPLHGTGEMLARRALAQAGFDSVQVVEAQATADPDFSTVTSP
                     NPESQAAFALAEELGRQVGADVLVATDPDADRVGVEVLQKDGSYLNLSGNQIGAIMAK
                     YILEAHKNAGTLPENAALCKSIVSTDLVTKIAESYGATMFNVLTGFKFIAEKIQEFEE
                     KHNHTYMMGFEESFGYLIKPFVRDKDAIQAVLVVAELAAYYRSRGLTLADGIEEIYKE
                     YGYYAEKTISVTLSGVDGAEQIKAIMAKFRNNAPTEWNATAITVVEDFKAQTATVADG
                     TVTNLTTPPSDVLKYTLADGSWIAVRPSGTEPKIKFYIAVVGETNEESQAKIANIEAE
                     INAFVK"
     gene            8526..8873
                     /locus_tag="VYN69_001554"
     CDS             8526..8873
                     /locus_tag="VYN69_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000434646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEV1533801.1"
                     /translation="MEQFLDNIKDLEVTTVVRAQEALDKKETATFFIGRKTCPYCRKF
                     AGTLSGVVAETKAHIYFINSEEPSQLNDLQAFRSRYGIPTVPGFIHITDGQINVRCDS
                     SMSAQEIKDFAGL"
     gene            9186..>9415
                     /locus_tag="VYN69_001555"
     CDS             9186..>9415
                     /locus_tag="VYN69_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1533802.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTG"
BASE COUNT         2725 a         1953 c         1738 g         2999 t
ORIGIN      
        1 gctctttgat tttcattgag tattagttca atatctaaaa aaagaacctt gccaagcaag
       61 attcttttag tgtctgactt aaataattgt tcttctggga actaaatcga attaagcttc
      121 gatttctgta accatacctg aaccaacagt acgtccaccc tcacggatag agaatgtagt
      181 accttgttct acggcgattg ggtgaatcaa ctcaacatcg attgtcacgt tatcaccagg
      241 cattaccatt tcagtacctg ctggaagttc gattgaacct gtaacgtcag tagtacggaa
      301 gtagaattgt ggacggtagt tgttgaagaa tggagtgtga cgtccacctt cttctttagt
      361 aaggatgtag acttcacctt tgaatttagt gtgtgggttg attgaacctg gtttagcgat
      421 aacttgtcca cgttcgattt catcacgttg aacaccacga agaaggacac ctacgttatc
      481 tccagcaaga ccttcgtcaa gttgtttacg gaacatttca acaccagtaa caactgcttt
      541 ttgagtttct tctttgatac caacgatttc gatttcgtcg ttgactttaa cgataccacg
      601 gtcgatacgt cctgaagcaa ctgtaccacg tccagtgatt gagaatacgt cctcgactgg
      661 aagaagcaat ggtttgtcag tgtcacgttc tggttctggg atatactcat caactgtgtt
      721 catcaattcc ataacgatgt cttcgtattt agagtcacct tcaagagctt taagtgctga
      781 accttggata actggaagat cgtcacctgg gaagtcgtat tctgacaata ggtcacggat
      841 ttccatttca accaattcaa gcaattcttc gtcgtcaacc aagtcaactt tgttcatgaa
      901 gacgataagg tgtttaacac caacctgacg tgaaagaagg atgtgctcac gagtttgtgg
      961 cattggtccg tcagttgaag ctactacaag gatagctccg tccatttgag cagcaccagt
     1021 gatcatgttt ttaacgtagt ccgcgtgtcc tggagcgtcg atgtgagcgt agtgacgttt
     1081 ttcagtttcg tactcaacgt gcgcagtgtt gatagtgata ccgcgttcgc gttcttctgg
     1141 agcagcatcg atagacgcat agtctttagg ttggttaact gatgaaggca agcgacgtgc
     1201 caaaacagtt gtgatagctg cagttagggt agttttaccg tggtcaacgt gtccgatagt
     1261 accaatgtta acgtgtggtt tactacgatc gtatttttct tttgccattt gagtaaaagc
     1321 ctccaataaa atatatttta tagatagaca gtaggcaata cagtctaact ttccttacta
     1381 ttttatcaaa tttcagctaa attgcaagtg ttttacaacg tttttgtgaa ttattcttgg
     1441 taaaaattta actaattaat agaaagtact aaatagactt atccttcaag gagttttcag
     1501 cattatccat ccactcactt gctgaaaatg taaacagtct ctggtttata tagtaaaatg
     1561 aaacaagaat agggcaaatc gttcaggaca gtcaaatcga tttctaacaa tgttttagaa
     1621 gtagatgtgt actattctag tttcaatcta ctatacaacc tcaaaactgt gttttgaaca
     1681 acctgcggct agcttcttag tttgcccttt gattttcatt gagtataaaa ttgcagataa
     1741 aacgataaca atcataggac tcagcagagt caatgtaggt cgaagaatag cccccctaat
     1801 cgcttatttt atagcaaaat gaaatagaaa ctgaacattt tgttgaggaa ggtaaatcaa
     1861 ttcctcacaa tgtttgagaa agaagggagg actattccaa cttcaatcca ctataaagca
     1921 gacttacaag atagggaacc catctccgat aatatatagt agaatgaaaa ctgcacaatt
     1981 tgattggaga attcaaacca atttctcaca atgttttaga aacaagggtc tattatttca
     2041 actgcaacct actatagccc attgaagctg gaatagccca ctgagacttc taaaccactg
     2101 ctgaagagga gtttgtttag agtttctttc atttccctaa aaaatcaaaa agagagaacc
     2161 agtctgatcc tctcttactt atagatggtg ctcactcaaa tgttaaagga gctatttctt
     2221 agagataaag gaatttgaat acagctactg ccgcaattgc tgctgcgata ggtgctacta
     2281 ctggtaccca agaataccac cattttgaat cgcctttatg ctcaccaaga actgattttg
     2341 gaaggaaagc atgaaggaga cgtggtccca agtcacgggc tgggttcaag gcaggtcctg
     2401 taggtcctcc aagtgatgtt accaaagcca taacgaggaa tccaagtgcc aagtgagcca
     2461 cagaaagtcc tgaagcagtg tgtggagcca cctgtgcttt aatagccaag tcagaaaaat
     2521 caactgtttg tcctgcttct attgcctttt gtttcatgaa ttgaagcact tcagcaccaa
     2581 agaagttttt agtcaaacca agagctgcaa agaacaaaac aaatgaacca acaaactcat
     2641 taatcaaacc attgacagtt gctgcatagc gactttcttt tgtaccatgg tcaatacttg
     2701 aaatagttga gaaagttccc aagatgttat ttgggttttc agttttcaag tagaatggac
     2761 ggtatgttgc cacaactaag gcttggccaa agatagcccc caagacttgc gcgataatgt
     2821 aaggtaccac ttgtgcccaa gggaaaagac cgctaactgc aagccctaga gtgaaagcag
     2881 ggttgatgtg attcccagat acgttaccaa acatcaaggc tgggatcata acccccatac
     2941 cataaccaac agcgatgacg atccagccac tttggtgacc tttcgtacct ttaagttcaa
     3001 cgttggcaac tgcaccattc ccaagaatga tcaaaatggc agttcccaaa aattcagtgg
     3061 catacttcag tgcccatgtg aaatccattt gctagattct ccttaaaatt ttttagatat
     3121 tccttatttt atcaattttt accctattta gcaactgatt tctggaaata tttttaaaaa
     3181 aataggattc atagtttccc agtccaacgt acttgtaagc gtttttattc ttcacgaaag
     3241 acctcattct caaaaccagg aaaaagccca catctgtgat cttttccttg ttcctagatt
     3301 ctaaaatcta ttatttttca tcttcttttt tcttgaagaa tcctaatcct agaccagcta
     3361 acaaggtcat gataccagca ctagccaagg tagcattagc ttctgtacct gtgtttggca
     3421 attcctctct cttacctgtc tcataagtcg gaacttctgg gtctggattc actggagttt
     3481 cagtttttgg agtacctggt tctggagttg gtttatctgg tgttgataaa cggtcatacc
     3541 ttaccgttat ttctttatca ctagagtctg acgtaacttc ttgtgattca actgttggaa
     3601 tatctggatc tttgtacttg tcaatcttac cagatataac ctcgtcccag tttcctgttg
     3661 tccattcacc gtaggttaca actcccgtga tcttgttctc agtttttgta cggcttaagg
     3721 ttacaggttg aacaacatct tcttttacat tttggttcgt aactttatca acgtaatgaa
     3781 tgatacgcgt tatagtcttc gtctcagtag aggttgctgt tttgggaacc actgtttcct
     3841 caacattctc acggtagtaa tagtcaactg ttgcaccgtc ttctggtacg catttgcagg
     3901 agttgctacc aaggtatatg ttgttttcct tgtgataact cggtcttctt tgtcctcagt
     3961 tgttgttttc ccttcaatag tttttgattc tgtggtatac tcaaaaccta tcgctaaatc
     4021 agcttttata acagactctg ccaacttctc ttggctacct tctttatagt aattggctgt
     4081 tactgtagca gtggttggcg cttcgctttc tctataaact aaggtcactg ttctaccttc
     4141 acttacaata ttcccagtta aacttgcaga atttgtatct gcttctttaa aagtataata
     4201 ttttccgtca gtagtagtca tgctactgag ttttttatct gtgacattgt agctggtacc
     4261 aatcagttgt tttttattgg taatgtaggt tccgtcactt tctttttctc caattccagt
     4321 atcatttttc aatgatagca aacgcccttg ttcatcaaca tagcgaactt tcacattttc
     4381 tgagattagt tctgccaatt ctgaggtttt tttctttttc ttgatttctt cggttatttt
     4441 ccctttctct tcttcgggaa tatttagttt tggaatgatt ttttcaacaa cggttcgtga
     4501 tggttccaca gtatcttgga tgactgaaaa gtcagctaga attgggagat tatactgaac
     4561 acggtgactt tgagtgttta ctcctactct ttcattattc tctgaaaata cttgtacggt
     4621 ataagaaaca acatcttttc ctaatagaac atccccagta gagaaatagc cgccttttcc
     4681 tagtttgcta tctccagagc ccacttcttt cctaatctta tcagatagtt ttttaccagt
     4741 cagtacattc gttcgcacaa tccctttgtc tacccctaca aagtgggaga actttttgaa
     4801 ctcttcagaa ccagatctag cccaaccatt attaagggca tttgcttttg tatttgtatt
     4861 ctctctcaaa agtttggcga ttagaattat attcatcggc acttagagtt gctgctatat
     4921 ctgactcttg aataccaact tccttactac catttctagc ggcagtatat gtgtatttat
     4981 ctgtttctac ttctagctga ttatccttaa catgataggt aattttctcc tggtataaga
     5041 gtcttcagta gtttatatac tctgtcatca tctttagctt tagaagcagc tgagtttagc
     5101 caagttgggg tattaggagt atctttatca gcctgtttca agacattatt caagtcattt
     5161 gctagcctat tggttatcct cactgtgaaa gttttcttcc agtctgaacc acttttatta
     5221 gacttagata tgctcatgaa atcctggtca acattcaaca caatatcgcc ttgtttttta
     5281 gcatcttccg ttgcttctgc ttcaaattga tcatggagtt tttgttgctc tggtgtatag
     5341 tttttttatt tacacgattt aatgtactat tttttatttt agtattaaga actccattaa
     5401 tacgattttt aaactctgct tcaaaaaatc ccttaatttg ttggtgcgca agttcaacat
     5461 tcaaaccttt cttatacaag tcttcattct gatacaagtc ttcattctga acgatttcgg
     5521 ctacacggtg aatggtttga ttgtataccg tcttctctta accagcagta tccttagttt
     5581 taatgactac tcgataggtt cctttagaac cgttatagtc atctagtttt aaaaaagcat
     5641 tccaaacatt caaaaatagt tggaacttcc cttaatacat ccgtcacttt ctttttcaag
     5701 atagcccaag cttgctcaat aggattcaaa tccggtgaat aaggtggtag aggtaagaaa
     5761 aagtgtttat cttggatgca gagttcatcc aaaatgttct tgggatgaaa accagcattg
     5821 cccatgacaa taacatgagg tgtcttcaaa gcaggtagga gttgcgtttt gaaccactcc
     5881 acaaagaaat cgcttgtcat gcttttcttg taaatcatgg gagctataaa ctctccgtct
     5941 acttgtcctg caacaattga agttcgctca aaacgacgtc cgctaatctt ttcatagact
     6001 ttctcccctc taggagcccc tgcataagga cgatagagat agcggtcgat tcctgtttca
     6061 tcaatataaa caggtgctag atgctttaaa ctattaaaat tcttaagaaa taaggctact
     6121 tttctgggtc ttgttcctta aagctcgtca tcttttttta aagtgacctt aatctgcttt
     6181 aaagctgccc atactgaagg aacagcacaa tcaaaatgtg ccgcaatttc ccgtaaaaaa
     6241 gcatctggat gagcctctac aaaggctttc aattcctcta aaggaatctt tcggtttttg
     6301 acgactcgct ttttcctctc tatgtgttct tgttcacgtc tttcttttcc cacgtgaaga
     6361 gagttctgac gccaacacca aaaaacttgg ctgcctcgac atggctgtgc ccctctttga
     6421 tgtaatctaa tgctcgctgt ttaaaatcag tactatatgc catagcttta ttataatatg
     6481 cttgtttaaa actaagtaac tatatcaatg ttttgttatg agaagactta ctttttttac
     6541 tgaaattgaa tttttcctag cctcaacatc atataacccc aaaattcaat ctgacctggt
     6601 ttgaagagat gttcgaagca gataaggttt tactaaaact aaaaaaaatg atataattaa
     6661 actgattaaa tgttttaaaa ataaaggaga atttgaatgt cttaccaaga aaattaccag
     6721 aaatgggttg attttgtgga gcttcctgac taccttcgtc aagatttgga aaatatggac
     6781 gaaaaaacta aggaagatgc cttctataca aatcttgaat ttggtactgc aggtatgcgt
     6841 ggcttggttg gtgctggtac aaaccgtatc aacatctacg ttgttcgcca agctactgaa
     6901 ggattggctc gtttgattga gtcaaaaggt ggaaacgaga aagaacgcgg tgtagcaatt
     6961 gcctacgata gccgtcactt ctcacctgag tttgcctttg aatctgcggc agttcttgct
     7021 aaacacggca tcaaatctta cgtatttgaa agccttcgtc caactccaga actatcattt
     7081 gcagttcgtc atctcaactg tttcgcaggt atcatggtca cagccagcca caaccctgct
     7141 ccatttaacg gttacaaggt ttacggtgaa gacggtggac aaatacctcc acacgatgcg
     7201 gacgctttga ctacttatat ccgtgcaatc gaaaacccat ttgcagttga agttgctgat
     7261 gtggaaactg aaaaagcttc tggcttgatt gaagttatcg gcgaagctgt tgacgtagaa
     7321 taccttaaag aggttaagga cgtaaacatc aacccagcct tgattgaaga atttggtaaa
     7381 gacatgaaga ttgtctacac accacttcat ggtactggtg aaatgttggc tcgtcgtgct
     7441 cttgcccaag caggatttga ctctgttcaa gttgttgaag cgcaagcaac tgctgaccca
     7501 gacttctcaa ctgtaacatc tccaaaccca gaaagccaag cagcctttgc ccttgctgaa
     7561 gaacttggtc gtcaagttgg tgcagatgtt cttgtcgcaa ctgacccaga cgctgaccgt
     7621 gttggtgttg aagttcttca aaaagatggt agctacctca acctttcagg taaccaaatc
     7681 ggtgctatca tggctaaata catcttggaa gctcacaaaa acgctggaac tcttcctgaa
     7741 aatgccgctc tctgcaaatc tatcgtttca actgacttgg taacgaagat tgctgaaagc
     7801 tacggcgcaa ctatgttcaa cgtcttgaca ggtttcaaat ttatcgctga aaaaatccaa
     7861 gaattcgaag aaaaacacaa ccacacttac atgatgggat ttgaagaaag cttcggttac
     7921 ttgattaaac catttgtacg tgataaagat gccatccaag ccgttcttgt cgttgctgaa
     7981 cttgctgcct actaccgttc tcgtggtttg acacttgctg acggtatcga agaaatctat
     8041 aaagaatatg gctactacgc agaaaaaaca atctctgtta ctctttcagg tgtcgatggt
     8101 gctgaacaaa tcaaagcgat tatggctaaa ttccgcaaca atgctccaac agaatggaac
     8161 gcaacagcta tcactgtcgt agaagacttc aaggcacaaa ctgctactgt tgctgacggt
     8221 actgttacaa acttgacaac tcctccaagt gatgtgttga aatacacact tgctgacggt
     8281 tcatggattg ccgttcgccc ttcaggtaca gaaccaaaaa tcaagttcta cattgcagtt
     8341 gtaggggaaa ccaacgaaga atcacaagct aagattgcta acatcgaagc agaaatcaat
     8401 gcatttgtaa aataaagacc tataaaagat gagaccaacc aacctcatct tttttcgtat
     8461 caaatctaag caattttaga aactttatgg catactagac ttatcaatga aagcgaggta
     8521 atctcatgga acaattttta gataacatca aagaccttga agtcactaca gttgtgcgtg
     8581 cgcaagaagc tcttgataaa aaagaaactg caaccttctt tatcggtcgc aaaacttgcc
     8641 cttactgccg taaatttgca ggtacattgt caggtgtcgt agctgaaacc aaagctcaca
     8701 tttacttcat caatagtgaa gaaccaagcc aactcaatga tttgcaagca ttccgctcac
     8761 gctatggaat cccaactgta ccaggtttta ttcatattac agatggacaa atcaacgtcc
     8821 gttgcgactc ttcaatgtca gcacaagaaa tcaaagattt cgcaggattg taaaaaatca
     8881 gctaacaagt cttaaaatca caaaaacgca taatgtaagg tgcaaaaacc ttgatattat
     8941 gcgttttatt gttcctaaaa cagttacatc agcatttcat tacagaacat tttaattttg
     9001 gaagtcaaat caatttttaa ccccaattat tcacccctaa tctaaaaacc atccagaatc
     9061 cttgccttag cttagatcct ggatggtttc ttttttcacc caatgggtgt tttttactag
     9121 acaaaaaaga gtttcccctt tatggtataa gtgtagaaaa aaacacaaaa agaaaggaaa
     9181 ctcacatgaa cagtttacca aatcatcact tccaaaacaa gtctttttac caactatctt
     9241 tcgatggagg tcatttaacc cagtatggtg gtcttatctt ttttcaggaa cttttttccc
     9301 agttgaaact aaaagagcgg atttctaagt atttagtaac gaatgaccaa cgccgctact
     9361 gtcgttattc ggattcagat atccttgtcc agttcctctt tcaactgtta acagg
//
LOCUS       DAVLVP010000069         9240 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.069, whole genome shotgun sequence.
ACCESSION   DAVLVP010000069 DAVLVP010000000
VERSION     DAVLVP010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9240
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.069"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            451..1926
                     /locus_tag="VYN69_001556"
     CDS             451..1926
                     /locus_tag="VYN69_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000661022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEV1533803.1"
                     /translation="MKELDQNQAPIYEALVKLRKKRIVPFDVPGHKRGRGNPELVELL
                     GEKCVGIDVNSMKPLDNLGHPISIIRDAEELAADAFGASHAFLMIGGTTSSVQTMILA
                     TCKAGDKIILPRNVHKSAINALVLCGAIPIYIEMSVDPKIGIALGLENDRVAQAIKDH
                     PDAKAILINNPTYYGICSDLKGLTEMAHEAGMMVLVDEAHGAHLHFTGKLPISAMDAG
                     ADMAAVSMHKSGGSLTQSSLLLIGEQMNPEYVRQIINLTQSTSASYLLMASLDISRRN
                     LALRGKESFEEVIELSEYARHEINAIGGYYAYSKELIDGVSVCDFDVTKLSVYTQGIG
                     LTGIEVYDLLRDEYDIQIEFGDIGNILAYISIGDRIQDIERLVGALADIKRLYSRDGK
                     DLIAGEYIQPELVLSPQEAFYSARKSLTLDDSVGQVCGEFVMCYPPGIPILAPGERIT
                     REIVDYIQFAKERGCSLQGTEDPEVNHINVIKRKTNYKKSQ"
     gene            complement(1892..2607)
                     /locus_tag="VYN69_001557"
                     /pseudo
     CDS             complement(1892..2607)
                     /locus_tag="VYN69_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809094.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            2863..3723
                     /gene="speE"
                     /locus_tag="VYN69_001558"
     CDS             2863..3723
                     /gene="speE"
                     /locus_tag="VYN69_001558"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HEV1533804.1"
                     /translation="MDLWFSEVHTPDVKLSLRTAKQLYAGKSEWQDIEVLDTPAFGKI
                     LILNGHVLFSDADDFVYNEMTVHVPMAVHPNPKKVLVIGGGDGGVAQVLTLYPELEQI
                     DIVEPDEMLVEVCREYFPDFAAGLDDPRVTIYYQNGLRFLRNCEDDYDIIINDATDPF
                     GHTEGLFTKEFYGNSYRALKEDGIMIYQHGSPFFDEDESACRSMHRKVNQAFPISRVY
                     QAHIPTSPAGYWLFGFASKKYHPVKDFDKEGWKKRQLFTEYYTANLHVGAFMLPKYVE
                     DILEEEEGKK"
     gene            3720..4979
                     /locus_tag="VYN69_001559"
     CDS             3720..4979
                     /locus_tag="VYN69_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase family protein"
                     /protein_id="HEV1533805.1"
                     /translation="MSRLLVIGCGGVAQVAISKICQDSETFTEIMIASRTKSKCDDLK
                     AKLEGKTSTKIETAALDADKVEEVITLIESYKPEAVLNVALPYQDLTIMDACLATGVH
                     YIDTANYEAEDTEDPEWRAIYEKRCKELGFTAYFDYSWQWAYQEKFKEAGLTALLGSG
                     FDPGVTSVFSAYALKHYFDEIHYIDILDCNGGDHGYPFATNFNPEINLREVSAPGSYW
                     EDGKWVEVEAMSIKREYDFPQVGQKDMYLLHHEEIESLAKNIPGVKRIRFFMTFGQSY
                     LTHMKCLENVGLLRTDTINFNGQEIVPIQFLKALLPDPASLGPRTVGKTNIGCIFTGV
                     KDGVEKTIYIYNVCDHQECYAEVGSQAISYTTGVPAMIGTKLVMNGTWKQAGVYNLEE
                     LDPDPFMEALNEYGLPWVVVENPQMVD"
     gene            4979..6106
                     /gene="nspC"
                     /locus_tag="VYN69_001560"
     CDS             4979..6106
                     /gene="nspC"
                     /locus_tag="VYN69_001560"
                     /EC_number="4.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxynorspermidine decarboxylase"
                     /protein_id="HEV1533806.1"
                     /translation="MKLEQVPTPAYVIDLAKLEANCRILQYVQEEAGCKVLLAQKAYS
                     LYKTYPLISQYLSGTTASGLYEAKLAREEFPGEVHVFAPAFKDEDMEELLEISDHIVF
                     NSERQLRKHGARCREAGVSVGLRLNPQCSTQGDHTLYDPCAPGSRFGVTIDKIPSDLL
                     DLVDGLHFHTLCEQGADDLQTTLKAVEEQFGPYLHEVKWLNMGGGHHITREGYDVDLL
                     ISEIKRIRKTYNLEIYIEPGEAIALNAGYLATEVLDIVENGMEILVLDASATCHMPDV
                     LEMPYRPPLRNGFEAQEKAHTYRLSSNTCLTGDVIGDYSFENPVQIGDRLYFQDMAIY
                     SFVKNNTFNGIGLPSLYLMDEQGDCSLLKAFGYQDFKGRLS"
     gene            6103..7188
                     /gene="aguA"
                     /locus_tag="VYN69_001561"
     CDS             6103..7188
                     /gene="aguA"
                     /locus_tag="VYN69_001561"
                     /EC_number="3.5.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatine deiminase"
                     /protein_id="HEV1533807.1"
                     /translation="MMDSPKKLGYHMPAEYEPHHGTLMIWPTRPGSWPFQGKAAKRSF
                     TQIIETIAEGERVYLLVEQAYLSEAQSYLGDKVVYLDIPTNDAWARDTGPTILVNDKG
                     KKLAVDWAFNAWGGTYDGLYQDYEEDDQVASRFAEALEKPVYDAKPFVLEGGAIHSDG
                     QGTILVTESCLLSPGRNPNLTKEEIENTLLESLGAEKVIWLPYGIYQDETNEHVDNVA
                     AFVGPAEVVLAWTDDENDPQYAMSKADLELLEQETDAKGCHFTIHKLPIPAVRQVVTE
                     EDLPGYIYEEGEEKRYAGERLAASYVNFYIANKAVLVPQFEDVNDQVALDILSKCFPD
                     RKVVGIPARDILLGGGNIHCITQQIPE"
     gene            7198..8073
                     /gene="aguB"
                     /locus_tag="VYN69_001562"
     CDS             7198..8073
                     /gene="aguB"
                     /locus_tag="VYN69_001562"
                     /EC_number="3.5.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001246759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-carbamoylputrescine amidase"
                     /protein_id="HEV1533808.1"
                     /translation="MRNVRVATIQMQCAKDVATNIQTAERLVRQAAEQGAQIILLPEL
                     FEHPYFCQERQYDYYQYAQSVAENTAIQHFKVIAKELQVVLPISFYEKDGNVLYNSIA
                     VIDADGEVLGVYRKTHIPDDHYYQEKFYFTPGNTGFKVWNTRYAKIGIGICWDQWFPE
                     TARCLALNGAELLFYPTAIGSEPILDTDSCGHWQRTMQGHAAANIVPVIAANRYGLEE
                     VTPSEENGGQSSSLDFYGSSFMTDETGAILERAERQEEAVLLATYDLDKGASERLNWG
                     LFRDRRPEMYRQITD"
     gene            complement(8254..9063)
                     /locus_tag="VYN69_001563"
     CDS             complement(8254..9063)
                     /locus_tag="VYN69_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEV1533809.1"
                     /translation="MIKLLALDMDGTLLNEAKEIPQAHIAAIHQAIEKGVKLVLCTGR
                     PLFGVLPYYKKLGLDLQNEYVIVNNGCSTHQTSDWGLVDWQELSPADIEYLYDLAEKS
                     DVQLTLFDESHYFVLGGKPNQVIENDAKLVFSDLTEISLEEATSGKFRMFQGMFLGTK
                     EQTDDFEQRFAEELCQRFSGVRSQPVIYEAMPLGTTKATALSRLAEILKIDSSEIMAM
                     GDANNDIEMLQFAGLGIAMGNASDYVKSLADAVTSSNEEDGVARAIEKYIL"
BASE COUNT         2703 a         1789 c         2075 g         2673 t
ORIGIN      
        1 tttttatttt ttatcaaaaa atactttaca agttcttaaa aacatgatat agtaataaag
       61 cttagaaaat gagatgatgt tttctagcaa atataaaccc gagtaaaaaa tgcctacgga
      121 caggcagggt tgaatgccga agcgtggttg aaaagccaca ttattgatag ggttaaaagc
      181 ctacttttat aagttgatgt taggacactt gtcctaattc ataaattttt agtgtggtga
      241 aagcacacgt catcttgtga aacgatcaat aaagtacgta atatttgcta ctagagagtt
      301 aggaaacatc gggaacagac atactcaaca gaaaccaaaa taaacacgtc agaagattgc
      361 agagcaggtg aaaacctgct cttttttcat gagtcaacct ttagttcctt agttttcata
      421 aggtcctaaa aatattgaaa ggagtatgtt ttgaaagagt tagatcaaaa ccaagcccca
      481 atttatgagg ccttggtgaa gttacgcaag aaaaggattg ttccctttga tgttccaggt
      541 cacaagcgtg gacggggaaa tccagaactt gtcgaactct taggagaaaa atgtgtaggc
      601 attgatgtca attcgatgaa acccttggat aatttaggcc atcctatttc gattattcgt
      661 gatgcagagg agctggctgc agatgctttt ggagctagcc atgcctttct aatgattggt
      721 ggaacaactt catcggtgca gactatgatt ctggcaacct gcaaggcagg agataagatt
      781 attctgccac gaaatgtcca taaatctgct atcaatgcgt tggttctatg tggtgccatt
      841 cccatctata tcgagatgag tgtagatcct aagattggta tcgctttagg tcttgaaaat
      901 gaccgagtag cacaggccat aaaggaccat ccagatgcta aggctatcct aatcaacaat
      961 cctacttact acggcatctg ttcagaccta aaggggttga cagaaatggc tcatgaagct
     1021 ggcatgatgg ttttagtaga tgaagcccat ggagcgcatt tgcatttcac tggtaaactt
     1081 ccaatttctg ctatggatgc aggagctgat atggcagcag tttccatgca taagtctggt
     1141 gggagtttga cacaaagttc gcttctttta atcggggagc agatgaatcc cgagtacgtt
     1201 cgtcagatta taaacctgac ccagtctaca tctgcctctt acttattgat ggctagtttg
     1261 gatatttcac gtcgcaactt ggcccttcgt ggtaaagagt cgtttgagga agtcattgag
     1321 ctatccgagt acgctcgtca tgaaatcaat gccattggcg gttactatgc ttactcaaaa
     1381 gagttaatag atggtgtgtc ggtctgtgat tttgatgtaa ccaagttgtc agtttacact
     1441 cagggaatag gcttaacagg tatcgaggtt tatgacctct tgcgagacga atacgacatt
     1501 cagatcgagt ttggtgatat cggcaatatc ttggcctata tttccatcgg cgaccgcatc
     1561 caagacatcg agcgcttggt tggtgctctg gctgatatta agagactcta ttcaagagat
     1621 ggaaaagatt tgatagcagg agaatatatt cagcccgagt tagtgctgtc tccgcaagaa
     1681 gccttctatt cagcaagaaa aagtttaact ttggatgatt ctgttggaca ggtctgtgga
     1741 gaatttgtta tgtgttaccc tccaggtatt cctatcttgg ctcctggtga acgcattaca
     1801 cgagaaattg tcgactatat ccaattcgcc aaggaacgtg gttgctccct ccaagggacg
     1861 gaagatccag aggtcaatca tatcaacgtt attaagagaa agacaaacta taagaaaagt
     1921 caatagttta tctaaactat ttcttatttc aatttgatga tttggcgatg attttagagc
     1981 acggcaaaaa gcccttgaat tagaagcggt caatcgctta atttctatca gcttatcaaa
     2041 tcctgcctca agccttttct gaggattagg gtagcgtgtc aagagttggt aggtatattc
     2101 tgaatgcttt ccaacgattt tatccaactc aggaaagatg atatcaagac aacgagtgta
     2161 ttgtactttc caatcagact gttttttctt gagacgatga atatgtctag ccagtatttt
     2221 tagttctact tgccgattat cgtgttgaaa ttgttcacga ttggggtcag aaagaagttt
     2281 aagagcgatg ccatgagcgt ctttcttatc cgttttagtc ttgcgaagtg ataatgattt
     2341 ggcaaatttc ttgatgagca aaggattgta ggtgtaaact ttatatcctt gttcatgcag
     2401 gaagttcagt agattaaagg cataatgtcc agtatcttca agagcgatga gacagtcttg
     2461 gttgagctgt cgaagagaca gatctaagag ttcaaaacca gctttattat ttgaaaaagt
     2521 gagtggttta agaacagttt ttccttgaac attcaaggct gtaacatcgt gtttattttt
     2581 agcgacatca atgcccacat aaggcatggg agtacctcca gatgtagtat ttcaagtcta
     2641 cttggttatc cacgaacttt ttgccttgtt accttagacg agataaaacg tctatgcgtt
     2701 atcaaactca ttaccaattg aaacaaaaag ctgtggttag agcctttcgg aaatcgtcaa
     2761 gcgattggag gaaatgaact aatccacagt ggcttattcc aagtatacca cttgggcttt
     2821 ggcagtagct aactgcgcta aatataatat aaggagaaat agatggattt atggttttct
     2881 gaagttcata ctccagatgt caaattatct ctgagaacag ccaagcaact ttacgctgga
     2941 aaaagtgaat ggcaggatat cgaagtcttg gatacgccag cttttgggaa aatactgatt
     3001 ttaaatggcc atgtcttgtt ctcagatgcg gatgatttcg tctacaatga aatgaccgtc
     3061 cacgttccca tggctgtcca cccaaatcca aagaaagtat tggttattgg gggtggtgac
     3121 ggcggtgttg cccaagtatt aacactctat cctgaactgg aacaaatcga tatcgtggaa
     3181 ccggatgaga tgttggtcga ggtctgtcgt gagtatttcc cagactttgc tgcaggccta
     3241 gatgatcctc gtgttactat ttactaccaa aatgggctac gctttttgcg aaactgcgaa
     3301 gatgactatg atattatcat caacgatgcg acagatccat ttggccatac ggaaggactc
     3361 tttaccaagg aattctacgg caatagttat cgagctctga aggaagacgg catcatgatt
     3421 taccagcatg ggagtccctt ctttgacgag gatgagtcgg cctgccgaag catgcaccgc
     3481 aaggtcaatc aagcctttcc aatcagtcgg gtttatcagg cccatattcc aactagccca
     3541 gctggctatt ggttgtttgg atttgcatcg aaaaaatacc accctgtcaa agattttgac
     3601 aaggaaggct ggaaaaaacg ccagcttttc acagaatact acactgcaaa cttacacgtg
     3661 ggagccttta tgttgcccaa gtatgttgag gacattttag aagaagagga aggaaaaaaa
     3721 tgagtcgttt actagttatt ggttgtgggg gcgttgccca agttgctatt tcaaagattt
     3781 gtcaagatag cgaaacgttt acagagatta tgattgctag ccgtaccaag tcaaaatgcg
     3841 atgacttgaa agcgaagcta gaaggcaaaa caagtactaa aattgaaact gcagcacttg
     3901 atgctgacaa ggtagaagag gtcattacct tgattgaaag ctacaaacca gaagctgttt
     3961 tgaatgtagc tctgccttat caagatttaa ccattatgga tgcttgtttg gcaacaggtg
     4021 ttcactatat cgatacagcc aactacgaag cagaagacac agaagaccct gagtggcgtg
     4081 ctatctacga aaaacgttgt aaggaacttg gttttacagc ctactttgac tactcatggc
     4141 agtgggctta tcaagagaaa ttcaaagaag caggcttgac tgctcttctt ggttctggtt
     4201 ttgacccagg tgtaactagt gtcttttcag cttatgccct caaacactat tttgatgaaa
     4261 tccattatat cgacatttta gactgtaatg gcggtgacca cggttatcca tttgcaacca
     4321 actttaatcc agaaattaat ctccgtgagg tttctgcgcc aggttcttac tgggaagatg
     4381 ggaaatgggt cgaagtcgaa gctatgtcta tcaagcgtga gtatgatttc cctcaagttg
     4441 gacaaaaaga tatgtatctc cttcaccatg aagaaatcga atcattggcc aagaacattc
     4501 caggtgtcaa acgcattcgt ttctttatga cttttggtca atcttacttg acgcacatga
     4561 aatgtcttga aaatgttgga ctccttcgta cggataccat taactttaac ggccaagaaa
     4621 ttgttccaat tcaatttttg aaagccttgc ttccagatcc tgccagtctt gggccacgta
     4681 cagtcggaaa aaccaatatt ggatgtatct ttacaggtgt caaagatggc gttgaaaaga
     4741 ctatctacat ctacaatgtt tgcgaccatc aagagtgcta tgcagaagtc gggtcacaag
     4801 caatttcata cacgacaggc gttccagcca tgattgggac aaaattagtc atgaacggaa
     4861 cttggaaaca agctggagtg tataaccttg aggagttaga tccagatcca ttcatggaag
     4921 ctttgaatga gtatggtttg ccatgggttg tggttgaaaa tccacaaatg gtggactaat
     4981 gaagttagaa caagtaccaa caccagccta tgttattgac ttggccaagt tagaagctaa
     5041 ttgccgcatt ctacaatatg tacaagaaga ggccggttgc aaggtcttgc ttgcccagaa
     5101 ggcatattcc ctctacaaaa cttatccctt gattagccag tatctatcag gtacgacagc
     5161 tagtggactc tatgaggcca aattggcaag ggaagaattt cctggtgaag tccatgtttt
     5221 tgcaccagct ttcaaggatg aggacatgga ggaactgctt gagatttcag accacattgt
     5281 ttttaattct gagagacagt tgcgtaagca tggggctcgt tgtcgagagg ctggtgtcag
     5341 tgttggtttg cgcctcaacc ctcagtgttc aactcaagga gatcacacgc tctatgaccc
     5401 ttgtgcacct ggttctcgct ttggagttac tatagacaag attccgagtg atttgctaga
     5461 tttggttgac ggacttcatt ttcataccct ttgcgagcag ggagcagatg atttacaaac
     5521 aactttgaaa gcagtagaag aacagtttgg tccctactta catgaggtaa aatggctcaa
     5581 tatgggtggt ggtcatcata ttacaagaga aggttacgat gtggatttgc tgatttcaga
     5641 aatcaagcgt atccgaaaaa cttacaatct tgaaatctat atcgagcctg gtgaagccat
     5701 tgcgcttaat gcgggttatt tagcaactga ggtattagat attgtagaaa atggtatgga
     5761 aatcttggtt ttagacgcct ctgcgacctg ccatatgcct gatgtacttg agatgcccta
     5821 tcgtccacct ttgagaaatg gctttgaggc acaggaaaaa gcccatacct acagactttc
     5881 ttctaatacc tgtctgacgg gcgatgtgat tggtgattat agttttgaaa atccagtcca
     5941 aatcggagac agactttatt ttcaagacat ggccatttat tcttttgtca aaaataatac
     6001 ctttaatggt attggattgc caagtctcta tctcatggac gaacagggag actgtagctt
     6061 actcaaagct tttggctatc aagactttaa agggagatta tcatgatgga cagtccaaaa
     6121 aaattaggct atcacatgcc agcagagtac gaaccccatc atggtaccct catgatatgg
     6181 ccgactcgac caggatcatg gccttttcaa ggaaaggctg ctaaaagatc atttactcag
     6241 atcatcgaga ccatagcaga aggggaaaga gtctatcttt tggtggagca ggcctatcta
     6301 tctgaagccc aatcctatct tggagacaag gttgtttatt tagacattcc caccaatgat
     6361 gcctgggcgc gtgatactgg cccaaccatt ctcgtcaatg ataaaggtaa gaaattagcc
     6421 gtggattggg ccttcaatgc ttggggaggc acctatgatg gtctttatca agattatgaa
     6481 gaggatgacc aagtagccag tcgttttgct gaggccttgg aaaagcctgt ctatgatgct
     6541 aaaccttttg tactggaagg aggcgcaatc catagcgatg gtcaaggaac tattctcgta
     6601 actgaaagtt gcttgcttag tcctggtcgc aatcctaacc tgactaaaga ggagattgaa
     6661 aacacattat tagaaagtct tggtgctgaa aaagttattt ggcttcctta tggtatttat
     6721 caggatgaaa ccaatgaaca cgtcgataat gttgctgcct ttgttggtcc tgctgaggtt
     6781 gttttagctt ggacagatga cgaaaatgat ccccagtatg ccatgtcaaa agcagatctc
     6841 gaactcttag aacaggaaac agatgcaaaa ggttgtcact tcaccattca taaattgcct
     6901 atccctgcag ttcgacaagt tgtgacagaa gaagatttgc caggctacat ctatgaagaa
     6961 ggagaagaaa agcgatacgc aggtgaacga ctagcagctt cctacgtaaa cttttatatc
     7021 gccaacaagg ctgtcttggt tccacagttt gaggatgtaa acgaccaagt ggccttagat
     7081 atcctcagca agtgtttccc agaccgtaaa gttgtcggaa taccagccag agatattctc
     7141 ttaggtggtg gcaatatcca ctgtatcacc caacaaattc cagaatagga gaaaaagatg
     7201 agaaatgtaa gagttgcaac cattcagatg caatgcgcta aggatgtggc aacaaatatc
     7261 caaaccgcag agcgtttagt acgtcaggct gctgagcaag gagcccaaat tattctcttg
     7321 cccgagttgt ttgaacatcc ctatttctgt caggaacgtc agtatgacta ctaccagtat
     7381 gcccaatctg tagcggaaaa tactgccatt cagcatttta aggtgattgc taaggaacta
     7441 caagttgttt taccaatcag tttctatgaa aaagatggta atgtcttgta taactctatt
     7501 gccgttattg atgcagatgg ggaagtgctg ggcgtttatc gaaagaccca cataccagat
     7561 gaccattatt atcaagaaaa attctatttc acgcctggta acactggttt caaggtctgg
     7621 aatactcgct atgctaagat tggtatcggt atctgttggg atcaatggtt ccccgaaaca
     7681 gcccgctgtc ttgctctgaa tggggcagaa ttgctctttt atccaacagc catcggttca
     7741 gagccgattt tggatacaga tagttgtggt cactggcaac gtactatgca agggcacgca
     7801 gcagcgaata ttgttccagt catcgcagcc aatcgttatg gtttagagga ggttactcct
     7861 agtgaggaaa atggcggaca gagttccagt cttgacttct acggttcctc ctttatgacg
     7921 gatgaaacag gagctattct agaacgagct gaaagacaag aagaagctgt tctgttagct
     7981 acttatgacc tagacaaggg agcgagtgaa cgcctaaact ggggcttgtt tcgagataga
     8041 agaccagaaa tgtatagaca aattacagat tagtgtggga gaaatgagag attcattctg
     8101 ctagactaac ttcttattag caactataag atactatggc atctagtaaa tcgattttta
     8161 tgattcgcta ttcttctcta ttgattagtc cgtattttaa aatattagca aaaaagcaaa
     8221 tagcagtaac ttctgtctat ttgcttttct tttttataga atatatttct caatagcacg
     8281 cgcaacgccg tcttcttcgt tgcttgaggt aacggcatcc gcaagagatt tgacataatc
     8341 gctggcattt cccattgcaa tcccaagccc tgcaaactgg agcatttcga tatcgttatt
     8401 agcatcgccc atggccataa tctctgagga atcaatcttc aaaatctcag ctagtcgtga
     8461 aagagcagta gcctttgtcg ttccaagcgg cattgcttca taaatgacag gctgcgaacg
     8521 aactccactg aatcgttggc aaagctcttc agcaaaacgc tgctcaaaat cgtctgtttg
     8581 ttcttttgtt cctaaaaaca taccttggaa catccggaac tttccactag tcgcttcttc
     8641 aagagaaatt tcagtcaggt ctgaaaatac tagtttagca tcattttcaa taacttgatt
     8701 gggcttgcca ccgagaacaa aataatgtga ctcgtcaaaa agtgtcaact gaacatcact
     8761 cttttcagca aggtcataga ggtattcgat gtcagctgga ctcagttctt gccagtcaac
     8821 caagccccag tcgctagtct ggtgagttga acaaccgttg ttaacaatca catactcatt
     8881 ctggaggtcg agtcccagtt ttttgtagta ggggaggaca ccgaaaaggg ggcgacccgt
     8941 acagaggacc agtttgacac ctttttcaat ggcttggtga atagcagcga tgtgagcctg
     9001 tgggatttcc ttggcttcat tgaggagggt tccatccata tccaaggcta gtagtttaat
     9061 cataggtctt ccttctttat cttttggtat tattatagca tattttggag aagaaattga
     9121 tagaaagttt gagactaatt gattttatag tttaagatgt tttgatgaca attcatgatt
     9181 tgaagaggat atttggcaaa gatatgctat actatgtttg tcaatgttgc aactagacaa
//
LOCUS       DAVLVP010000070         9040 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.070, whole genome shotgun sequence.
ACCESSION   DAVLVP010000070 DAVLVP010000000
VERSION     DAVLVP010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 9040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9040
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.070"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..182
                     /locus_tag="VYN69_001564"
     CDS             <1..182
                     /locus_tag="VYN69_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533810.1"
                     /translation="FLIELFTSYFNSLYFPLYSMKIKAQTRKLATGCSKHCFEVVDKT
                     DEVSSKYCFEVADGS"
     gene            complement(366..1214)
                     /gene="dprA"
                     /locus_tag="VYN69_001565"
     CDS             complement(366..1214)
                     /gene="dprA"
                     /locus_tag="VYN69_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEV1533811.1"
                     /translation="MKITNYEIYKLKKSGLTNQQILKVLEYGENVDQELLLGDIADIS
                     GCRNPAVFMERYFQIDDAHLSKEFQKFPSFSILDDCYPWDLSEIYDAPVLLFYKGNLD
                     LLKFPKVAVVGSRACSKQGVKSVEKVIQGLENELVIVSGLAKGIDTAAHMAALQNGGK
                     TIAVIGTGLDVFYPKANKRLQDYIGNDHLVLSEYGPGEQPLKFHFPARNRIIAGLCRG
                     VIVAEAKMRSGSLITCERAMEEGRDVFAIPGSILDGLSDGCHHLIQEGAKLVTSGQDV
                     LAEFEF"
     gene            complement(1310..1999)
                     /locus_tag="VYN69_001566"
     CDS             complement(1310..1999)
                     /locus_tag="VYN69_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HEV1533812.1"
                     /translation="MKAIILAAGLGTRLRPMTENTPKALVQVNQKPLIEYQIEFLKEK
                     GINDIIIIVGYLKEQFDYLKEKYGVRLVFNDKYADYNNFYSLYLVKEELANSYVIDAD
                     NYLFKNMFRNDLTRSTYFSVYREDCTNEWFLVYGDDYKVQDIIVDSKAGRILSGVSFW
                     DAPTAEKIVSFIDKAYASGEFVDLYWDNMVKDNIKELDVYVEELEGNSIYEIDSVQDY
                     RKLEEILKNEN"
     gene            complement(2011..2889)
                     /locus_tag="VYN69_001567"
     CDS             complement(2011..2889)
                     /locus_tag="VYN69_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000837620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HEV1533813.1"
                     /translation="MKSKNGVPFGLLSGIFWGLGLTVSAYIFSIFTDLSPFVVAAAHD
                     FLSIFILLVFLLVKEGKVRLSIFLNIRNVSVIIGALLAGPIGMQANLYAVKYIGSSLA
                     SSVSAIYPAISVLLAFFFLKHKISKNTVFGIVLIIGGIIAQTYKVEQVNSFYIGILCA
                     LVCAIAWGSESVLSSFAMESELSEIEALLIRQVTSFLSYLVIVLFSHQSFVAVANGQL
                     LGLMIVFVAFDMISYLAYYIAINRLQPAKATGLNVSYVVWTVLFAALFLGTSLDMLTI
                     MTSLVVIAGVYIIIKE"
     gene            complement(2879..3748)
                     /gene="licA"
                     /locus_tag="VYN69_001568"
     CDS             complement(2879..3748)
                     /gene="licA"
                     /locus_tag="VYN69_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline kinase LicA"
                     /protein_id="HEV1533814.1"
                     /translation="MEKIIKEKISSLLSQEEEVLSVEQLGGMTNQNYLAKTTNKQYIV
                     KFFGKGTEKLINRQDEKYNLELLKDLGLDVKNYLFDIEAGIKVNEYIESAITLDSTSI
                     KTKFDKIAPILQTIHTSAKELRGEFAPFEEIKKYESLIEEQIPYANYESVRNAVFSLE
                     KRLADLGVDRKSCHIDLVPENFIESPQGRLYLIDWEYSSMNDPMWDLAALFLESEFTS
                     QEEETFLSHYESDQTPVSHEKIAIYKILQDTIWSLWTVYKEEQGEDFGDYGVNRYQRA
                     VKGLASYGGSDEK"
     gene            complement(3765..4787)
                     /locus_tag="VYN69_001569"
     CDS             complement(3765..4787)
                     /locus_tag="VYN69_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004257983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEV1533815.1"
                     /translation="MINQIYQLTKPKFINVKYQEEAIDQENHILIRPNYMAVCHADQR
                     YYQGKRDPKILNKKLPMAMIHESCGTVISDPTGTYEVGQKVVMIPNQSPMQSDEEFYE
                     NYMTGTHFLSSGFDGFMREFVSLPKDRVVAYDAIEDTVAAITEFVSVGMHAMNRLLTL
                     AHSKRERIAVIGDGSLAFVVANIINYTLPESEIVVIGRHWEKLELFSFAKECYITDNI
                     PEDLAFDHAFECCGGDGTGPAINDLIRYIRPQGTILMMGVSEYKVNLNTRDALEKGLI
                     LVGSSRSGRIDFENAIQMMEVKKFANRLKNILYLEEPVREIKDIHRVFATDLNTAFKT
                     VFKWEV"
     gene            complement(4792..5499)
                     /locus_tag="VYN69_001570"
     CDS             complement(4792..5499)
                     /locus_tag="VYN69_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016465903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HEV1533816.1"
                     /translation="MIYAGILAGGTGTRMGISNLPKQFLELGNRPILIHTIEKFVLEP
                     SIEKIVVGVHGDWVSHAEDLVDKYLPLYKERIIITKGGADRNTSIKKIIEAIDAYRPL
                     TPEDIVVTHDSVRPFITLRMIQDNIQLAQNHDAVDTVVEAVDTIVESTNGQFITDIPN
                     RAHLYQGQTPQTFRCKDFMDLYGSLSDEEKEILTDACKIFVIKGKDVALAKGEYSNLK
                     ITTVTDLKIAKSMIEKD"
     gene            5835..7322
                     /gene="tacF"
                     /locus_tag="VYN69_001571"
     CDS             5835..7322
                     /gene="tacF"
                     /locus_tag="VYN69_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV teichoic acid flippase TacF"
                     /protein_id="HEV1533817.1"
                     /translation="MKSIKLNALSYMGIRVLNIIFPILTGTYVARVLDRTDYGYFNSV
                     DTILSFFLPFATYGVYNYGLRAISNVKDNKKDLNRTFSSLFYLCIACTILTTAVYILA
                     YPLFFTDNPIVKKVYLVMGIQLIAQIFSIEWVNEALENYSFLFYKTAFIRILMLVSIF
                     LFVKNEHDIVVYTLVMSLSTLINYLISYFWIKRDIKLVKIHLSDFKPLFLPLTAMLVF
                     ANANMLFTFLDRLFLVKTGIDVNVSYYTIAQRIVTVIAGVVTGAIGVSVPRLSYYLGK
                     GDKEAYVSLVNRGSRIFNFFIIPLSFGLMVLGPNAILLYGSEKYIGGGILTSLFAFRT
                     IILALDTILGSQILFTNGYEKRITVYTVFAGLLNLGLNSLLFFNHIVAPEYYLLTTML
                     SETSLLVFYIIFIHRKQLIHLGHIFSYTVRYSLFSLSFVAIYFLINFMYPVDMVINLP
                     FLINTGLIVLLSAISYISLLVFTKDSIFYEFLNHVLALKNKFKKS"
     gene            7332..8135
                     /locus_tag="VYN69_001572"
     CDS             7332..8135
                     /locus_tag="VYN69_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004259727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1533818.1"
                     /translation="MKQLTVEDAKQIELEILDYIDTLCKKHNINYIINYGTLIGAVRH
                     EGFIPWDDDIDLSMPREDYQRFINIFQKEKSKYKLLSLETDKNYFNNFIKITDSTTKI
                     IDTRNTKTYESGIFIDIFPIDRFDDPKVIDTCYKLESFKLLSFSKHKNIVYKDSLLKD
                     WIRTAFWLLLRPVSPRYFANKIEKEIQKYSRENGQYMAFIPSKFKEKEVFPSGTFDKT
                     IDLPFENLSLPAPEKFDTILTQFYGDYMTLPPEEKRFYSHEFHAYKLED"
     gene            8137..8946
                     /locus_tag="VYN69_001573"
     CDS             8137..8946
                     /locus_tag="VYN69_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphorylcholine transferase LicD"
                     /protein_id="HEV1533819.1"
                     /translation="MQYLEKKEIKEIQLALLDYIDETCKKHDIPYFLSYGTMLGAIRH
                     KGMIPWDDDIDISLYREDYERLLKIIEEENHHRYKVLSYDTSSWYFHNFASILDTSTV
                     IEDHVKYKRHDTSLFIDVFPIDRFTDLSIVDKSYKYVALRQLAYIKKSRAVHGDSKLK
                     DFLRLCSWYALRFVNPRYFYKKIDQLVKNAVTNTPQYEGGVGIGKEGMKEIFPVDTFK
                     ELILTEFEGRMLPVPKKYDQFLTQMYGDYMTPPSKEMQEWYSHSIKAYRKN"
BASE COUNT         2846 a         1821 c         1517 g         2856 t
ORIGIN      
        1 ctttcctgat agagttgttc acatcttatt tcaattcact atactttccc ttatactcaa
       61 tgaaaatcaa agcgcaaact aggaagctag ccacaggttg ctcaaagcac tgctttgagg
      121 ttgtagataa gactgacgaa gtcagctcaa aatactgttt tgaggttgca gatggaagct
      181 gacgtagttt gaagagattt tcgaagagta ttatatgaga aaaatcctct actctactaa
      241 tccacttaga aaaaacgcat attatcaagt gttaaaaact tgctaatatg cgttttatta
      301 tggaaatgtt gggttcattt tctcttaaaa ttgatttttt cttagaattt tagcttaggt
      361 catttttaaa attcaaattc cgcaagaaca tcttgcccac tggtgaccaa ttttgctcct
      421 tcttgaatca aatgatggca accgtctgat agtccatcta aaatgctacc aggaatagca
      481 aagacatcgc gtccttcttc cattgctcgc tcacacgtaa tgagactacc tgaacgcatc
      541 ttagcctctg ctacaatcac accacgacaa agtccagcaa tgatgcgatt acgggcagga
      601 aaatgaaatt tcagaggttg ttcgccaggt ccatattcac ttagaaccag atggtcattg
      661 ccgatgtagt cttgcaagcg tttattggct ttaggataaa acacatccag tcctgttcca
      721 atcactgcaa tggtttttcc gccattctga agagctgcca tatgagctgc tgtgtcaatg
      781 cccttggcca gaccactgac aataaccagt tcattttcca agccttgaat gactttttca
      841 actgatttaa ctccctgttt gctacaagca cgactgccca cgaccgctac cttcgggaat
      901 ttcaggaggt caagatttcc cttgtaaaat aaaagtacag gcgcatcata tatttcactc
      961 aaatcccaag gataacagtc atctaaaata gagaaagatg gaaatttttg aaactctttc
     1021 gacaaatgcg catcgtctat ctgaaaataa cgttccataa aaacggctgg attacggcaa
     1081 cctgatatat ctgcaatatc acccaacaaa agctcctgat caacattttc accgtattct
     1141 agaactttca aaatctgttg attggtcaaa cctgattttt ttaacttata gatttcatag
     1201 tttgtgattt tcataaataa ctccatttct ttttctactc atctatctat tcgtaaaagt
     1261 tcgtaaaaaa tcaaaaaaca tccgactatt ttgtcagatg ttggaatctt taattttcgt
     1321 ttttaagaat ttcttctaat ttacgatagt cttggacact atcgatctca taaatgctat
     1381 tgccttctaa ttcttcaaca tagacatcta gctctttgat attatcctta accatattgt
     1441 cccagtagag atcaacaaat tcgccacttg cataagcctt atcgataaag ctgacaatct
     1501 tttctgcagt tggagcatcc cagaaggata caccactaag gatgcgacct gccttgctat
     1561 caacaataat gtcttgaacc ttgtagtcat ctccatagac caagaaccat tcgttggtac
     1621 aatcttcacg ataaacacta aaataagtcg aacgtgtcaa atcattgcgg aacatatttt
     1681 taaagagata attgtcagca tcaataacat agctgttggc caattcttct tttacaagat
     1741 agagagagta aaagttattg tagtcagcgt atttatcatt gaaaacgagg cgaacaccgt
     1801 atttttcttt taaataatcg aattgttctt taagataacc aacgatgatg atgatgtcat
     1861 tgattccttt ttctttgaga aactcaattt ggtattcaat caaaggtttt tgattaacct
     1921 gaaccaaggc tttaggggta ttttcagtca taggacgcaa gcgagttccc aatcccgctg
     1981 ctaagataat ggctttcaca cgaatctcct ttattcttta ataataatat aaactccagc
     2041 aatgacgaca agtgacgtca taatggtcag catatctaat gatgttccca agaaaagagc
     2101 tgcgaacaag acagtccata ctacatagct cacgttcaag cctgtagcct tggctggttg
     2161 caagcgattg atagcgatat aataagccaa ataggaaatc atatcaaagg ctacaaagac
     2221 aatcatgaga cctagcaatt gtccattggc tactgcaaca aatgactgat gagagaagag
     2281 cacaatcaca agataggaca agaacgaagt tacttgacgg attaagaggg cttcgatttc
     2341 actcaattca ctttccatgg caaaagagct aagaacactc tcacttcccc atgcaatagc
     2401 acaaaccaaa gcacaaagaa tcccaatgta gaaagaatta acctgttcaa ccttataggt
     2461 ctgggcaata atccctccaa taatcaagac aatcccaaat acagtatttt tcgaaatctt
     2521 gtgcttcaaa aagaagaaag ccaatagaac tgaaatcgca gggtaaatag ccgatacaga
     2581 tgaagctaaa gaacttccga tatacttaac tgcataaaga ttggcctgca taccgatagg
     2641 gcctgctagc aaggctccta tgataacact gacattgcga atatttaaga aaattgagag
     2701 gcgaactttc ccttctttta ccaagagaaa aactagtaag ataaagatgc tcaaaaaatc
     2761 atgagctgca gccaccacaa agggtgacaa atctgtaaaa atcgaaaaga tataagcact
     2821 aaccgttaga cccaagcccc agaaaatacc tgatagaagg ccaaaaggaa ctccgttttt
     2881 acttttcatc tgaacctcca taagaagcca aacctttaac agctctttgg taacgattca
     2941 caccatagtc accaaaatct tcaccttgct cttccttata gacagtccat agactccaaa
     3001 tagtatcttg taaaatttta taaatagcaa tcttttcatg agaaaccggt gtttggtcac
     3061 tctcatagtg agataagaaa gtttcttcct cttgggaagt gaattcagac tctaaaaaga
     3121 gggcagccaa atcccacatt ggatcattca ttgatgaata ttcccagtca atcaaataaa
     3181 gtcgtccttg aggtgattcg ataaagtttt caggcaccaa atcgatatga caagattttc
     3241 tgtcaacacc taagtcagcc agtcttttct ctaaggagaa gactgcattt ctaacagatt
     3301 catagttggc ataaggaatt tgttcttcaa tcaaggattc gtattttttg atttcttcaa
     3361 aaggagcaaa ttctcctctt aattccttag cagacgtatg aatagtttgt aatattggag
     3421 caattttgtc gaacttggtc ttgattgacg ttgaatcaag cgtaatcgca gattcgatat
     3481 actcatttac tttgatacca gcttcaatat caaaaagata attttttaca tctaagccta
     3541 aatcctttag tagttcaaga ttgtactttt catcttgtcg attgataagc ttttctgtcc
     3601 ctttaccaaa gaatttaaca atgtattgct tatttgttgt tttggccaaa tagttttgat
     3661 tggtcattcc acccagttgt tcaacactga ggacttcctc ttcttgacta agtaaggaag
     3721 aaattttttc tttaatgatt ttctccacaa ttaacctcca gtacttatac ttcccactta
     3781 aacactgttt taaaggctgt gtttaaatcg gttgcaaaga cacgatgaat atctttaatt
     3841 tctcttacag gttcttctag ataaaggata tttttaagac gattggcaaa tttcttgact
     3901 tccatcattt ggatagcatt ttcaaaatca atgcgaccag aacgagatga cccaaccaaa
     3961 atcaagccct tttctaaggc atcgcgagta ttgagattga ctttatattc gctaactccc
     4021 atcatgagaa tcgttccctg aggacgaatg tagcgaatca agtcattaat agctggtcca
     4081 gtaccatcac caccacaaca ttcaaaagca tggtcaaagg ccaaatcttc aggaatatta
     4141 tccgtaatat agcattcttt ggcaaatgag aagagttcca acttttccca atgacgacca
     4201 ataaccacaa tctctgattc tggcaaagta tagttgataa tattggcaac cacaaaagct
     4261 aaacttccat ctccaataac ggcgatccgc tcccgcttgc tatgagcaag agtcaataga
     4321 cgattcatag cgtgcatgcc cacactgaca aactctgtaa tggctgcaac cgtatcttca
     4381 atagcatcat aagccaccac acgatcttta gggagagaaa caaactctct cataaagcca
     4441 tcaaatccac tagacaagaa atgggtccct gtcatgtagt tttcatagaa ttcttcatca
     4501 ctctgcatag gagactgatt gggaatcatg acaacttttt gaccaacctc gtaggttccg
     4561 gtcgggtcag aaatgacggt tccacatgac tcgtgaatca ttgccattgg aagcttttta
     4621 ttcaaaatct tgggatcacg ttttccctga tagtaacgct gatccgcatg acagacagcc
     4681 atgtagttgg gacggataag gatatgattc tcttggtcaa tagcctcttc ctgatatttg
     4741 acattgataa acttaggctt agttagttga taaatttgat taatcatttt actagtcttt
     4801 ctcaatcata ctttttgcaa tcttcaaatc tgttacggtt gtaatcttca gatttgagta
     4861 ttcacctttg gccaaggcca catcttttcc tttgatcaca aagattttac atgcatctgt
     4921 caagatttcc ttctcttcat cagaaagaga tccataaagg tccatgaagt ccttgcaacg
     4981 gaatgtttga ggtgtttgtc cttgataaag gtgagcacga tttggaatat ctgtaataaa
     5041 ttgaccattg gtactttcaa cgatagtatc aaccgcttct accactgtgt ccactgcgtc
     5101 atgattttgg gcaagttgga tattgtcctg aatcatgcga agtgtaataa atggacgaac
     5161 agaatcgtgg gtaacaacga tatcctctgg agtaagcgga cgataagcat caatggcttc
     5221 aatgattttc ttaatacttg tattgcggtc agcaccaccc tttgtaatga tgatacgttc
     5281 cttataaaga ggaagatatt tatctacaag atcttctgca tgagaaaccc agtctccatg
     5341 aacaccaact acaatttttt caatacttgg ctccaagaca aatttttcaa ttgtatgaat
     5401 caaaataggt cgattaccta gctctaaaaa ttgttttggc aagttactga tccccatgcg
     5461 tgtgccagtt ccaccggcaa gaattcctgc ataaatcatc tattttctcc tttgtcttac
     5521 taaaaaaact tattctctct attataccac aatctagtcc tatcataaaa gaaatactgg
     5581 tcctcccctt ctagaatagg agaattatgc agttctatta ttcaaagaaa ctagtccatt
     5641 tctagtaatg actaggaatt ctgtagcaat tactaataaa tggctgcgaa attttagagt
     5701 ccttcagaat aatatacttt ccttaaagaa aacttaaaac ttaagatttg aaacttcaaa
     5761 gtgattactt gacttactgc ccttcatttc ttattagcta actttatgat ataatgaaaa
     5821 acgaggtaaa ttgaatgaaa agtataaaat taaatgctct atcttacatg ggaattcgtg
     5881 tcttgaatat tatttttccc atcctaactg gaacctatgt cgcgcgtgtc ttggaccgaa
     5941 ctgactatgg ttacttcaac tcagtcgaca ctattttgtc atttttcttg ccctttgcaa
     6001 cttatggtgt ctataactac ggtttaaggg ctatcagtaa tgtcaaggat aacaaaaaag
     6061 atcttaacag aaccttttct agtctttttt atttgtgcat cgcttgtacg attttgacca
     6121 ctgctgtcta tatcctagcc tatcctctct tctttactga taatccaatc gtcaaaaagg
     6181 tctaccttgt tatggggatt caactcattg cccagatttt ttcaatcgaa tgggtcaatg
     6241 aagctctgga aaattacagt tttctctttt acaaaactgc cttcatccgt atcctgatgc
     6301 tggtctctat tttcttattt gttaaaaatg aacacgatat tgttgtctat acacttgtga
     6361 tgagtttatc gacgctgatt aactacctga ttagttattt ttggattaaa agagacatca
     6421 aacttgttaa aattcaccta agtgatttta aaccactctt tctccctctg acagccatgt
     6481 tagtctttgc caatgccaat atgctcttca cttttttaga tcgcctcttc ctcgttaaaa
     6541 cagggattga tgtcaacgtt agttactata ccatagctca gcgaattgtg accgttatag
     6601 ctggggttgt aacaggtgca attggagtga gtgtgcctcg tctcagttac tatctgggga
     6661 aaggagacaa agaagcctat gtttctctgg ttaatagagg tagtcgaatc tttaacttct
     6721 ttatcattcc actgagtttt ggactcatgg ttttaggacc aaatgccatc ctactttacg
     6781 gtagtgaaaa atatatcgga ggcggcatct tgacctctct cttcgctttt cgtacgatta
     6841 tcctggcctt agataccatt cttggttccc aaattctctt taccaatggc tatgaaaaac
     6901 gtatcacagt ctatacagtc tttgctgggc tactcaattt gggcttgaat agtctcctct
     6961 ttttcaacca tatcgtggct cctgaatact acttactgac aactatgtta tcagagactt
     7021 ctctacttgt tttctatatc attttcatcc atagaaaaca actcatccac ttgggacata
     7081 tctttagcta tactgttcga tactctctct tttcactttc ctttgtagca atttatttcc
     7141 tgattaattt catgtatcct gtggatatgg tcattaattt gccatttttg attaatactg
     7201 gtttgattgt cttgctatca gctatctctt atattagtct acttgtcttc acaaaagata
     7261 gcattttcta tgaattttta aaccatgtcc tagccttaaa aaataaattt aaaaaatcat
     7321 aggagtttaa aatgaaacaa ctaaccgttg aagatgccaa acaaattgaa ttagaaattt
     7381 tggattatat tgatactctc tgtaaaaagc acaatatcaa ctatattatt aactacggta
     7441 ctctgattgg ggcggttcga catgagggct ttatcccttg ggacgacgat attgatctgt
     7501 ccatgcctag agaagactac caacgattta ttaacatttt tcaaaaggaa aaaagcaagt
     7561 ataagctcct atccttagaa actgataaga actactttaa caactttatc aagataaccg
     7621 acagtacgac taaaattatt gatactcgaa atacaaaaac ctatgagtct ggtatcttta
     7681 tcgatatttt ccctatagat cgctttgatg atcctaaggt cattgatact tgttataaac
     7741 tggaaagctt caaactgctg tctttcagta aacataaaaa tattgtctat aaggatagcc
     7801 ttttaaaaga ttggatacga acagcctttt ggttgctcct tcgaccagtt tctcctcgtt
     7861 attttgcaaa taaaatcgag aaagaaattc aaaaatatag tcgtgaaaat gggcagtata
     7921 tggcttttat cccttcaaaa tttaaggaaa aggaagtctt cccaagtggt acctttgata
     7981 aaacaatcga tttacccttt gagaatttaa gccttcctgc acctgaaaaa tttgatacta
     8041 ttttgacaca attttatgga gattatatga ccctaccacc agaagaaaaa cgcttctaca
     8101 gtcatgaatt tcacgcttat aaattggagg attaggatgc aatatttaga aaaaaaagaa
     8161 attaaagaaa ttcaactagc cctgctggac tatattgatg agacttgtaa gaaacatgat
     8221 attccttatt ttctcagtta tggaaccatg cttggagcca tccgccacaa aggtatgatt
     8281 ccttgggatg atgatattga tatttccctt tatcgtgagg attatgagcg tttactgaag
     8341 attattgaag aagaaaatca ccatcgctac aaggttcttt cctacgatac atcttcttgg
     8401 tacttccata atttcgcatc gattttggac acttctactg ttatagaaga ccatgttaag
     8461 tacaagcgtc atgataccag ccttttcatc gatgtcttcc caattgatcg atttacagac
     8521 ttgagcattg tcgacaagag ctataagtat gtggcccttc gtcaactagc ttatatcaaa
     8581 aaatcacgag cagttcacgg tgatagcaaa ctaaaagatt ttcttagatt atgtagctgg
     8641 tacgctctcc gatttgtcaa tcctcgctac ttttacaaga aaattgatca actagtcaaa
     8701 aatgctgtaa ccaacactcc tcaatatgaa ggaggagttg ggatcggtaa ggaagggatg
     8761 aaagaaatct tcccagttga tacctttaaa gaactgattt taactgagtt tgagggccgt
     8821 atgttgcctg ttcccaaaaa atatgatcaa tttttaaccc agatgtatgg cgattatatg
     8881 acaccaccat caaaagaaat gcaagagtgg tatagtcata gcattaaagc ttatcgcaaa
     8941 aactgattga gggggattat acaaactact aagatagagg ttattcaaaa acataatttt
     9001 agtagaaaat gaaatacata ttcccacaat aaaacgcatc
//
LOCUS       DAVLVP010000071         8961 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.071, whole genome shotgun sequence.
ACCESSION   DAVLVP010000071 DAVLVP010000000
VERSION     DAVLVP010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8961
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.071"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(318..947)
                     /locus_tag="VYN69_001574"
     CDS             complement(318..947)
                     /locus_tag="VYN69_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1533820.1"
                     /translation="MVLSKYYGVADGMNVEGRGSANFIKDNVLITAAHNYYRHDYGKE
                     ADDIYVLPAVSPSQELFGKIKVKEVRYLKEFRNLNSKDAREYDLALLILEEPIGAKLG
                     TLGLPTSQKNLTGITVTITGYLSYNFKIHQMYTDKKQVLSDDGMFLDYQVDTLEGSSG
                     STVYDASHRVVGVHTLGDGANQINSAVKLNERNLPFIYSVLKGYSLEGW"
     gene            complement(992..1231)
                     /locus_tag="VYN69_001575"
     CDS             complement(992..1231)
                     /locus_tag="VYN69_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533821.1"
                     /translation="MKKTVYKKLGISIIASTLLASQLSTVSALSVISSTGEEYEVSET
                     LEKGPESNDSSLSEISPTYGSYYQKQSEVLSVMMI"
     gene            complement(1381..1647)
                     /locus_tag="VYN69_001576"
                     /pseudo
     CDS             complement(1381..1647)
                     /locus_tag="VYN69_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000060522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(1672..1983)
                     /locus_tag="VYN69_001577"
     CDS             complement(1672..1983)
                     /locus_tag="VYN69_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004250000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEV1533822.1"
                     /translation="MDWDFADTCWEKEPRKYQYVAANYLKAMQSYLKETDLPKLEQLV
                     VTKSWWDTVDILDRVVGSLVYEHPELEEIILILFENLFKPRQLYLQPQNSVLSNLRLA
                     S"
     gene            complement(1908..2150)
                     /locus_tag="VYN69_001578"
                     /pseudo
     CDS             complement(1908..2150)
                     /locus_tag="VYN69_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812384.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
     gene            complement(2151..2783)
                     /locus_tag="VYN69_001579"
     CDS             complement(2151..2783)
                     /locus_tag="VYN69_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1533823.1"
                     /translation="MKILLVDDHEMVRLGLKSYFDLQDDVEVVGEASNGSQGIDLALE
                     LRPDVIVMDIVMPEMNGIDATLAILKEWPEAKILIVTSYLDNEKIMPVLDAGAKGYML
                     KTSSADELLHAVSKVAAGELAIEQEVSKKVEYHRNHMELHEELTARERDVLQLIAKGY
                     ENQRIADDLFISLKTVKTHVSNILAKLEVSDRTQAAVYAFQHHLVGQDEF"
     gene            complement(2797..3792)
                     /locus_tag="VYN69_001580"
     CDS             complement(2797..3792)
                     /locus_tag="VYN69_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000743650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1533824.1"
                     /translation="MKKQAYVIIALTSFLFVFFFSHSLLEILDFDWSIFLHDVEKTEK
                     FVFLLLVFSMSITCLLALFWRGIEELSLRKMQANLKRLLAGQEVVQVADPDLDASFKS
                     LSGKLNLLTEALQKAENHSLAQEEEIIEKERKRIARDLHDTVSQELFAAHMILSGISQ
                     QALKLDREKMQIQLQSVTAILETAQKDLRVLLLHLRPVELEQKSLIEGIQILLKELED
                     KSDLRVSLKQNMTKLPKKIEEHIFRILQELISNTLRHAQASCLDVYLYQTDVELQLKV
                     VDNGIGFRLGSLDDLSYGLRNIKERVEDMAGTVQLLTAPKQGLAVDIRIPLLDKE"
     gene            complement(3789..4487)
                     /locus_tag="VYN69_001581"
     CDS             complement(3789..4487)
                     /locus_tag="VYN69_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEV1533825.1"
                     /translation="MRKFKIFLFIEACLLTGALILMVSEHFSRFLLILFLFLLLIRYY
                     TGKEGNNLLLVAATILFFFIVMLNPFVILAIFVAVIYSLFLLYPMMNQEKEQTNLVFE
                     EVVTVKKEKNRWFGNLHHFSSYQTCQFDDINLFRFMGKDTIHLERVILTNHDNVIILR
                     KMVGTTKIIVPVDVEVSLSVNCLYGDLTFFNQPKRALRNEHYHQETKDYLKSNKSVKI
                     FLTTMIGDVEVVRG"
     gene            complement(4564..5574)
                     /locus_tag="VYN69_001582"
     CDS             complement(4564..5574)
                     /locus_tag="VYN69_001582"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEV1533826.1"
                     /translation="MTTNRKDEHILYALEQKSSYNSFDEVELIHSSLPLYNLDEIDLS
                     TEFAGRKWDFPFYINAMTGGSNKGREINQKLAQVAESCGILFVTGSYSAALKNPTDNS
                     FSVKSSHPNLLLGTNIGLDKPVELGLQTVEEMNPVLLQVHVNVMQELLMPEGERKFRS
                     WQSHLADYSKQIPVPIVLKEVGFGMDAKTIERAYEFGVRTVDLSGRGGTSFAYIENRR
                     SGQRDYLNQWGQSTMQALLNAQEWKDKVELLVSGGVRNPLDMIKCLVFGAKSVGLSRT
                     VLELVETYTVEEVIGIVQGWKADLRLIMCSLNCATIADLQKVDYLLYGKLKEANDQMK
                     KA"
     gene            complement(5558..6565)
                     /locus_tag="VYN69_001583"
     CDS             complement(5558..6565)
                     /locus_tag="VYN69_001583"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000562378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEV1533827.1"
                     /translation="MIAVKTCGKLYLAGEYAILEPGQLALIKDIPIYMRAEIAFSDSY
                     RIYSDMFDFAVDLRANPDYSLIQETIALMGDFLAVRGQNLRPFSLKICGKMEREGKKF
                     GLGSSGSVVVLVVKALLALYNLSVDQELLFKLTSAVLLKRGDNGSMGDLACIVAEDLV
                     LYQSFDRQKAAAWLEEENLATVLERDWGFFISQVKPTLECDFLVGWTKEVAVSSHMVQ
                     QIKQNINQNFLSSSKETVVSLVEALEQGKAEKVIKQVEVASKLLEGLSTDIYTPLLRQ
                     LKEASQDLQAVAKSSGAGGGDCGIALSFDAQSTKTLKNRWADLGIELLYQERIGHDDK
                     S"
     gene            complement(6552..7505)
                     /gene="mvaD"
                     /locus_tag="VYN69_001584"
     CDS             complement(6552..7505)
                     /gene="mvaD"
                     /locus_tag="VYN69_001584"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEV1533828.1"
                     /translation="MDREPVTVRSYANIAIIKYWGKKKEKEMVPATSSISLTLENMYT
                     ETTLSPLPANVTADEFYINGQLQNEVEHAKMSKIIDRYRPAGEGFVRIDTQNNMPTAA
                     GLSSSSSGLSALVKACNAYFKLGLDRSQLAQEAKFASGSSSRSFYGPLGAWDKDSGEI
                     YPVETDLKLAMIMLVLEDKKKPISSRDGMKLCVETSTTFDDWVRQSEKDYQDMLIYLK
                     ENDFAKIGELTEKNALAMHATTKTASPAFSYLTDASYEAMAFVRQLREKGEACYFTMD
                     AGPNVKVFCQEKDLEHLSEIFGQRYRLIVSKTKDLSQDDCC"
     gene            complement(7487..8365)
                     /gene="mvk"
                     /locus_tag="VYN69_001585"
     CDS             complement(7487..8365)
                     /gene="mvk"
                     /locus_tag="VYN69_001585"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEV1533829.1"
                     /translation="MTKKVGVGQAHSKIILIGEHAVVYGYPAISLPLLEVEVTCKVVS
                     AESPWRLYEEDTLSMAVYASLEYLDITEACVRCEIDSAIPEKRGMGSSAAISIAAIRA
                     VFDYYQADLPHDVLEILVNRAEMIAHMNPSGLDAKTCLSDQPIRFIKNVGFTELEMDL
                     SAYLVIADTGVYGHTREAIQVVQNKGKDALPFLHALGELTQQAEVAISQKDAEGLGQI
                     LSQAHLHLKEIGVSSPEADFLVETTLSHGALGAKMSGGGLGGCIIALVTNLTHAQELA
                     ERLEEKGAVQTWIESL"
     gene            complement(8487..8816)
                     /locus_tag="VYN69_001586"
     CDS             complement(8487..8816)
                     /locus_tag="VYN69_001586"
                     /inference="COORDINATES: protein motif:HMM:NF024736.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1533830.1"
                     /translation="MTRKAYDTDLNDQEWAKIEPYFSKHRTYKWPKRVLVNETLYVTK
                     TGCQWRMLPHDFPLYLMVWSFFHRSMTTGWFQVNGRWYYAYSSGALAVNTTVDGYSVN
                     YNGEWVQ"
BASE COUNT         2633 a         2081 c         1595 g         2652 t
ORIGIN      
        1 aacttttcgg actatccaaa aatacttcgt tattggacga ttacattata gtaaaacgaa
       61 ataagaacag gacaaatcga tcaggacagt caaatcaatt tctaacaatg ttttagaagt
      121 agaggtgtac tattatagtt tcaatctact atacaagacg aaattgaaga aatctttcca
      181 ttataaaacg catattatca aggtttttaa acacctaata atatgcgttt tttcctattt
      241 taaagacttt tatccaccta ttatacaaag ttaagtatcc atcacagttt acaattagcc
      301 ttcattacct aacccattca ccatccttca agagagtaac ctttaagaac cgaataaata
      361 aatggcaaat ttcgttcatt taatttaact gcactgttaa tttgattagc tccatctcct
      421 aaagtatgca ctcctactac acggtgacta gcatcataaa ctgtagatcc actagacccc
      481 tctaaagtat caacttggta atccaagaac atgccatcat cacttaaaac ttgtttttta
      541 tctgtataca tttgatgaat tttaaaatta tatgatagat agcctgtgat agtcacagtt
      601 attcctgtca aatttttttg actagtagga agacccaaag tccctaattt tgcaccaatg
      661 ggctcttcta gaattaataa agccaagtca tattcccttg catccttaga atttaaattt
      721 ctaaattcct tcaaataacg aacttccttt actttgatct ttccaaatag ttcttgactt
      781 ggactaacag ccggaagaac ataaatatca tccgcttctt tcccatagtc atgtctgtag
      841 tagttgtgag ccgctgtaat taacacatta tctttaataa aattcgcact tcccctccct
      901 tctacattca ttccatcggc tactccataa tacttagata aaaccatagc tgaagtcgaa
      961 tacggatact gtaaagtatt atcaatttta atcaaatcat cattaccgat aatacttctg
     1021 attgcttttg gtagtatgaa ccatacgttg gtgaaatctc agataatgaa gaatcattag
     1081 actctggacc tttttctagt gtctcactta cctcatattc ttcacctgta ctagaaataa
     1141 cactcaaagc agatactgtc gataactggc tagccaataa agtactcgca ataattgaaa
     1201 tacccaattt tttataaaca gttttcttca ttattgtatc ctcctaatgt aattatagcg
     1261 tactattcta aatttcttaa tctactatag aatcaagaaa tctaccacct tctttaaata
     1321 ccctccatta tcacataaac aggtaaactt ttcaattaat gactgcgctt ttcaatcacg
     1381 ctagaggtac ttacttgctt ctttgatact aagttcagcc attctttcct tgtttttctc
     1441 aataaagcat gttacccaag tgggattcgt tttggagtag tctcgcagag tccagccaat
     1501 ggctttattg ataaaaaatt ctgtttggtt caagttatga agtagaatct tttccattaa
     1561 ttgagtattg gtcttctctt ttcttaacaa ctggtggtca atagcgacac gtctcagcca
     1621 gatattatct gataggctcc attttatact caatgaaaat caaagagcaa actaggaagc
     1681 tagccgcaga ttgctcaaaa cactgttttg aggttgcaga tagagctgac gtggtttgaa
     1741 gagattttcg aagagtatta agattatttc ttctagttca gggtgttcat acaccaaact
     1801 ccctactact cgatctagga tatctaccgt gtcccaccag gattttgtca cgactaactg
     1861 ctctagctta ggcaaatcgg tttcctttag ataagactgc attgctttca aatagttagc
     1921 agccacatat tggtattttc taggctcctt ttcccagcaa gtgtctgcaa aatcccaatc
     1981 gataatcttt gtttttttcg cttctagtaa atatttttat agagtttatt tctttcaggc
     2041 accgcaatac ctagaaaaga aaattgatgg cgcatatagg cttccatgga ccttgctttt
     2101 ttagagtctt ttgctgcttc tagctcctca agtaaatctg ctaaactcat ctaaaactca
     2161 tcttgcccca ccaaatggtg ctgaaaggca tagacagccg cctgggtacg atcgctgact
     2221 tcaagtttgg caagaatgtt ggacacgtgg gtcttgaccg tcttgagaga gataaagagg
     2281 tcatctgcga tgcgctgatt ttcgtagccc ttggcgatga gttggagaac atctcgctca
     2341 cgcgcagtca attcttcatg aagttccata tgattgcggt ggtattcaac cttcttgcta
     2401 acctcttgct caatggccag ctcgccagca gctaccttac tgacggcatg aagcaattca
     2461 tctgcactag aagtcttgag catatagcct ttggcaccag catctaagac tggcatgatt
     2521 ttttcattgt ccaaataaga ggtcacaatc aaaatcttgg cttcaggcca ttctttaagg
     2581 attgctaagg tcgcgtcaat cccattcatc tcaggcatga caatatccat gacaatgaca
     2641 tctggacgca gttccaaggc caagtcaatc ccttgagacc cgttggacgc ctcacccaca
     2701 acttctacat cgtcttggag gtcaaagtag cttttcaaac ccaaacggac catttcatgg
     2761 tcatctacta gtaaaatttt catctttact cctttatcat tccttatcta acaggggaat
     2821 acggatatca actgccagcc cttgcttggg agctgtcaag agttgaactg ttccagccat
     2881 atcttcaacc ctctccttga tatttcgcag tccataactc aagtcgtcta agcttcctaa
     2941 ccggaaacca atcccattgt ccaccacctt cagttgcaat tcaacatctg tctgatagag
     3001 gtagacatct aggcaagatg cctgggcatg gcggagggta ttgctaatca actcttgcag
     3061 gatacggaag atatgctcct cgattttctt aggcaatttc gtcatattct gcttgagact
     3121 aaccctaaga tcactcttgt cctcaagctc ttttaagaga atttgaatcc cttctatcaa
     3181 gctcttctgc tccagttcaa ctggtcgcaa atgcaagagc aaaacccgca aatccttctg
     3241 ggcagtttct aaaatagctg tgacactctg caactggatc tgcatctttt ctctatccaa
     3301 tttcaaagcc tgctgactga tacccgataa aatcatgtgg gccgcaaaca actcctgact
     3361 gactgtatcg tgcaaatccc gagcaattcg cttccgttct ttctcgatga tttcctcttc
     3421 ctgagcaagg ctatgatttt cagctttttg aagagcttct gtcaaaaggt taagtttacc
     3481 tgataaggac ttgaaactgg catccaaatc tggatctgca acctgaacca cttcttgccc
     3541 tgccaataaa cgcttgagat tagcctgcat ttttcttaga gaaagctctt cgatccctcg
     3601 ccaaaacagg gctaagagac aggttatgga catgctgaaa accaacaata aaaagacaaa
     3661 tttttctgtt ttttcgacat cgtgcaaaaa gatagaccag tcaaaatcaa gtatttccag
     3721 caagctgtgg gagaaaaaaa agacaaacag gaaggaggtg agagcaataa tgacataggc
     3781 ttgttttttc atcctctaac cacctccaca tcaccaatca tagtggtcaa gaaaatcttg
     3841 acactcttgt tactcttgag atagtctttt gtttcttgat gatagtgttc attgcggagg
     3901 gctcgcttgg gctggttgaa aaaagtcaaa tccccataga gacagttaac gctgagactg
     3961 acttccacat ctacaggtac gatgattttg gtcgttccta ccatctttct gaggataatg
     4021 acattgtcat gattggttaa gatgactctc tccagatgaa tagtgtcctt gcccatgaag
     4081 cgaaagagat tgatatcatc aaattggcaa gtctggtagc ttgaaaaatg atgaagattt
     4141 ccaaaccaac gatttttctc cttcttaacc gtcacaacct cttcaaaaac caaattggtc
     4201 tgctcttttt cctggttcat catcgggtaa agaagaaaga gactatagat aaccgcaaca
     4261 aaaatagcta gaatcacaaa aggattgagc ataacaatga aaaagaagag aatggttgcc
     4321 gctactaaaa gaagattatt cccctcttta ccagtgtagt agcgaatcaa aagcaaaaag
     4381 aggaatagta tcagcagaaa acgcgaaaaa tgctctgata ccatcaaaat cagagctcct
     4441 gtcagaagac aggcttcgat aaataaaaag attttaaatt ttctcatagg ttcatcctct
     4501 cccttctatt ttatcacaat tcaaaaaagt cacctcagtc tgaggatgga aaaaaggcgc
     4561 tggttacgcc tttttcatct gatcatttgc ttcttttaat tttccataaa gaagatagtc
     4621 tactttttgt agatctgcta tggtggcaca gttaagggaa cacataatca agcgtaggtc
     4681 tgctttccag ccttggacaa tgccaatcac ttcttcaact gtgtaggttt caaccaattc
     4741 cagaacggtt cgtgacaatc ccacagactt agcaccaaaa accaagcact taatcatatc
     4801 cagcggattc cgaacccctc cactaaccaa gagttcgacc ttatctttcc attcttgggc
     4861 attgagaagg gcctgcatgg tagactgacc ccattgattg aggtaatcac gctggccact
     4921 acgacggttt tcgatatagg caaagctggt gccaccacga cccgataggt ccactgtacg
     4981 aacaccgaat tcataggctc tttcgattgt cttggcatcc attccaaagc ccacttcctt
     5041 gaggacaata ggaacgggaa tttgcttgct ataatctgct agatgcgatt gccagcttct
     5101 aaacttcctt tctccctcgg gcatgagtaa ttcctgcatg acattgacat gcacttgcaa
     5161 tagaacagga ttcatctctt ctacagtctg aagtcctaac tcgacaggtt tgtccaatcc
     5221 aatattggtt ccaaggagga gattgggatg actagacttg acagaaaaag aattatccgt
     5281 tggatttttg agggctgcgc tataagaacc cgttacaaat aaaataccac aggattccgc
     5341 cacctgagcc agcttttgat tgatttctct tcccttatta cttccaccag tcatggcatt
     5401 gatataaaaa ggaaagtccc actttcgacc agcaaactct gtcgaaagat cgatttcatc
     5461 cagattgtaa agaggcaagg aagaatgaat cagctccacc tcatcaaagc tattatagga
     5521 acttttctgc tcaagggcat agaggatatg ctcgtcctta cgatttgtcg tcatgtccta
     5581 tcctttcttg atataagagc tcaatcccca gatcggccca acgatttttt aaggttttgg
     5641 ttgattgcgc atcaaaactc agggcgatgc cacagtcacc accaccagca ccactactct
     5701 tggcaacggc ctgcaaatct tgactggctt ctttcaactg tctaagcaaa ggcgtgtaaa
     5761 tatctgtact caagccttct aaaagcttgc tggctacttc tacttgcttg ataacttttt
     5821 cggctttccc ctgctccaag gcttcgacca aagaaaccac cgtttctttt gaggaactta
     5881 aaaaattttg attgatattt tgcttgattt gctggaccat gtgactcgat acagccactt
     5941 ccttggtcca tcccactaag aaatcacatt ctaaagttgg tttcacttgt gagataaaaa
     6001 atccccaatc acgctccaga actgtcgcca agttttcttc ttctaaccaa gcagccgcct
     6061 tctggcgatc aaatgactgg tagagaacca aatcctctgc cacaatacag gcaaggtcgc
     6121 ccatggaacc attgtctcct cgcttgagca agacagcgct agtcagcttg aacaagagct
     6181 cctgatcaac cgaaagatta tagagagcca ataaagcctt gacaaccaag acaacgacgc
     6241 tgccactaga acctagacca aactttttcc cttctcgttc cattttgcca cagattttta
     6301 gggaaaaagg tcttaaattc tgaccgcgaa cagcgaggaa gtctcccatc aaagcaatcg
     6361 tttcttgaat caagctgtag tcaggattag cccttaagtc cactgcgaaa tcaaacatat
     6421 ctgaatagat acggtagctg tcagaaaaag caatctcagc cctcatatag atgggaatat
     6481 cctttatcaa agctaactgc cctggctcta aaatagcata ttcacctgcc aaatagagtt
     6541 ttccgcaagt tttaacagca atcatcttga ctcaaatcct ttgtttttga cacaatcaag
     6601 cgataacgct gaccgaaaat ttctgacaaa tgctccaagt ctttctcctg acagaagacc
     6661 ttaacattgg gaccagcatc catggtaaag tagcaggcct ctcctttctc acgaagctga
     6721 cgaacaaagg ccatagcctc ataagaggca tccgtcagat aagaaaaggc tggactagca
     6781 gtctttgtcg tagcatgcat agccagggca tttttctccg ttaattctcc aatcttggca
     6841 aaatcatttt ccttgagata aatcagcata tcctgatagt ccttctcaga ctgacgaacc
     6901 cagtcgtcga aagtcgtcga ggtttccaca caaagtttca tcccgtcacg gctagagatt
     6961 ggttttttct tgtcctctag caccaacata atcatagcta gtttcaagtc tgtctctaca
     7021 gggtaaattt ctccactatc cttatcccag gctcctagtg gtccataaaa actccgagaa
     7081 gaagagcctg aggcaaattt ggcttcctgt gccaactgac ttctatccaa tccaagtttg
     7141 aaataagcat tacaagcctt gaccagggcg gacaaaccac tagaacttga ggacaggccc
     7201 gctgccgtag gcatattgtt ttgagtatcg atacggacaa agccctcacc agctggacga
     7261 taacggtcaa taatcttact catcttggca tgctcgacct cattttgtag ctgaccattg
     7321 atgtaaaatt cgtcagctgt tacattggct ggtaaaggcg acaaggtcgt ctctgtatac
     7381 atattttcca aagttagaga aatactgcta gtagcaggca ccatctcttt ttcttttttc
     7441 tttccccaat atttgataat agcaatattt gcgtaggaac gtactgttac aggctctcta
     7501 tccatgtctg aacagctcct ttctcttcta atctttctgc tagttcttgt gcgtgtgtca
     7561 aattggttac caaggctatg atacaacctc ctagcccacc accgctcatc ttggcaccca
     7621 gagcaccatg gctaagagtc gtttcaacca aaaagtctgc ctcagggcta ctgactccaa
     7681 tttcttttaa atgtaaatgc gcttgactga ggatttgtcc cagtccttca gcatcttttt
     7741 gtgaaatcgc aacttctgct tgctgggtta attctcccaa ggcatgcaaa aacggtaggg
     7801 catccttgcc cttattttga accacttgga tggcttcacg agtatgacca taaacacccg
     7861 tatcggcaat caccaaatag gcggataaat ccatctcaag ttctgtaaat cctacgttct
     7921 tgataaagcg aataggttgg tcactgagac aggtcttagc atccaaacca ctaggattca
     7981 tatgggcaat catctcagct cgattgacca agatttctag tacatcatga ggcagatcag
     8041 cctgatagta gtcaaatacc gcacgaatgg ccgctatgct gatagccgct gacgaaccca
     8101 tcccccgttt ctcagggata gccgagtcaa tctcacaacg aacgcaggct tctgtgatat
     8161 ccaaatactc cagtgaggca taaaccgcca tggacaaggt atcctcctca taaaggcgcc
     8221 aaggactctc tgcagaaact accttacagg tcacctccac ctccaaaaga ggcagggaaa
     8281 tggcaggata accgtaaacg accgcatgtt cccctattaa aattatctta ctatgtgcct
     8341 gaccgacacc aacttttttt gtcatttttt ccttttacta gacgaaaaaa cgtcttattt
     8401 ttcatacaag tattaattct ttcctatcta ttttattata ttttcacaaa aaaagcgatt
     8461 gtttccattc acaatcgctt ctttcattat tgaacccatt cgccattata gttgacagaa
     8521 tagccatcta cggtcgtatt cactgccaag gcacctgagc tataagcgta gtaccatctg
     8581 ccattgacct ggaaccaacc tgtcgtcata gaacgatgaa agaaactcca taccattaag
     8641 taaagaggaa agtcgtgagg gagcatgcgc cattgacaac ctgttttagt gacgtacaaa
     8701 gtctcattaa caagtactcg tttcggccat ttataggtgc ggtgtttgga gaaatagggt
     8761 tcaatcttag cccattcttg atcatttaaa tcagtatcat atgctttgcg tgtcataact
     8821 ctagtttaac atttttttgt gaatagaggt tctaaataat cgaccacgaa aatttcttaa
     8881 gtgaaaaacg ccttatgaag tatgctacgg gaaagttatg cacttaattt gacaattcaa
     8941 gatgtaaaaa tatatactat a
//
LOCUS       DAVLVP010000072         8856 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.072, whole genome shotgun sequence.
ACCESSION   DAVLVP010000072 DAVLVP010000000
VERSION     DAVLVP010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8856
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.072"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(354..986)
                     /locus_tag="VYN69_001587"
     CDS             complement(354..986)
                     /locus_tag="VYN69_001587"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEV1533831.1"
                     /translation="MTLAKDIASHLLKIQAVYLKPEEPFTWASGIKSPIYTDNRVTLA
                     YPETRTLIENGFVEAIKEAFPEVEVIAGTATAGIPHGAIIADKMDLPFAYIRSKPKDH
                     GAGNQIEGRVAQGQKMVVVEDLISTGGSVLEAVAAAKREGADVLGVVAIFSYQLPKAD
                     KNFADAGVKLVTLSNYSELIHLAQEEGYITPEGLDLLKRFKEDQENWQEG"
     gene            complement(1020..1721)
                     /gene="pyrF"
                     /locus_tag="VYN69_001588"
     CDS             complement(1020..1721)
                     /gene="pyrF"
                     /locus_tag="VYN69_001588"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001206708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEV1533832.1"
                     /translation="MREHRPIIALDFPSFEAVKEFLALFPAEESLYLKVGMELYYAAG
                     PEIVSYLKGLGHSVFLDLKLHDIPNTVKSAMKVLSQLGVDMTNVHAAGGVEMMKAARE
                     GLGSQAKLIAVTQLTSTSEAQMQEFQNIQTSLQESVIYYAKKTAEAGLDGVVCSAQEV
                     QVIKQATNPDFICLTPGIRPAGAAVGDQKRVMTPADAYQIGSDYIVVGRPITQAEDPV
                     AAYHTIKDEWTQDWN"
     gene            complement(1954..3153)
                     /gene="divIB"
                     /locus_tag="VYN69_001589"
     CDS             complement(1954..3153)
                     /gene="divIB"
                     /locus_tag="VYN69_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DivIB"
                     /protein_id="HEV1533833.1"
                     /translation="MSKDKKNEDKEILEELKELSEWQKRNQEYLKKKAEEEAALAEEK
                     EKERQARMGEESEKSEDKQDQESETDQEDSESAKEESEEKVASSEADKEKEEKEESES
                     KEKEEQDKKLAKKATKEKPAKAKIPGIHILRAFTILFPSLLLLIVSAYLLSPYATMKD
                     IRVEGTVQTTADDIRQASGIQDSDYTINLLLDKAKYEKQIKSNYWVESAQLVYQFPTK
                     FTIKVKEYDIVAYYISGENHYPILSSGQLETSSVSLNSLPETYLSVLFNDSEQIKVFV
                     SELAQISPELKATIQKVELAPSKVTSDLIRLTMNDSDEVLVPLSEMSKKLPYYSKIKP
                     QLSEPSVVDMEAGIYSYTVADKLIMEAEEKAKQEAKEAEKKQEEEQKKQEEESNRNQT
                     TQRSSRR"
     gene            complement(3163..4221)
                     /locus_tag="VYN69_001590"
     CDS             complement(3163..4221)
                     /locus_tag="VYN69_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HEV1533834.1"
                     /translation="MKKIVFTGGGTVGHVTLNLLLMPKFIEDGWEVHYIGDKRGIEHQ
                     EILKSGLDVTFHSIATGKLRRYFSWQNMLDVFKVGWGIVQSLFIMLRLRPQTLFSKGG
                     FVSVPPVIAARVSGVPVFIHESDLSMGLANKIAYKFATKMYSTFEQASSLSKVEHVGA
                     VTKVSDQKNPEPDELVDIQSHFNHKLPTVLFVGGSAGARVFNQLVTDHKKELTERYNI
                     INLTGDSSLNELSQNLFRVDYVTDLYQPLMELADIVVTRGGANTIFELLAIAKLHVIV
                     PLGREASRGDQIENAAYFVKKGYAEDLQESDLTLDSLEEKLSHLLSHKEDYQAKMKAS
                     KELKSLADFYQLLKKDLS"
     gene            complement(4223..5575)
                     /locus_tag="VYN69_001591"
     CDS             complement(4223..5575)
                     /locus_tag="VYN69_001591"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEV1533835.1"
                     /translation="MKVIDQFKNKKVLVLGLAKSGESAARLLDKLGAIVTVNDGKPFE
                     DNPAAQSLLEEGIKVITGGHPLELLDEEFALMVKNPGIPYNNPMIEKALAKGIPVLTE
                     VELAYLISEAPIIGITGSNGKTTTTTMIGEVLTAAGQHGLLSGNIGYPASQVAQTASD
                     KDTLVMELSSFQLMGVQEFHPEIAVITNLMPTHIDYHGSFSEYVAAKWNIQNKMTAAD
                     FLVLNFNQDLAKDLTSKTEATVVPFSTLEKVDGAYLEDGQLYFRGEVVMAANEIGVPG
                     SHNVENALATIAVAKLRGVDNQTIKETLSAFGGVKHRLQFVDDIKGVKFYNDSKSTNI
                     LATQKALSGFDNSKVVLIAGGLDRGNEFDELVPDIPGLKKMVILGQSAERVKRAADKA
                     GVAYVEATDIADATRKAYEIATQGDVVLLSPANASWDMYANFEVRGDLFIDTVAELKE
                     "
     gene            complement(5679..6320)
                     /locus_tag="VYN69_001592"
     CDS             complement(5679..6320)
                     /locus_tag="VYN69_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533836.1"
                     /translation="MIELKNISKKFGSRQLFSDTNLHFEGGKIYALIGTSGCGKTTFL
                     NMIGRLEPYDKGQIIYDGTSLKDIKPSVFFRDYLGYLFQDFGLIENQTVKENLNLGLV
                     GKKLKEKEKISLMKQALNRVNLSYLDLKQPIFELSGGEAQRVALAKIILKDPPLILAD
                     EPTASLDPKNSEELLSILESLKNPNRTIIIATHNPLIWEQVDQVIRVTDLSHR"
     gene            complement(6322..8343)
                     /locus_tag="VYN69_001593"
     CDS             complement(6322..8343)
                     /locus_tag="VYN69_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000732428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1533837.1"
                     /translation="MKKLFILLSTFFLSFFLAWIIVLRAPQYLYASYDSVSLLRVKKD
                     TQEPTREVFEQELENFANSEQSLIARRIVEPSKDGTTHFTYATYGQGTLPKEFQEASQ
                     ESRERSDPLNSYLLLSGSLTKEKLADKLGDLGYKASADRKIPPYFLAFRILLNPLILI
                     SLAIFGLSFFALVIITRIKEMRAAGIKLFSGQTLLSIMGHSLSTDIKWLLLSALLSFL
                     GGGVVLFSQGLFYPILLATYGFGISFYLLFLLAISILLMLLYLMSLSYKALVPVIKGR
                     LPLKRLMILTLLCQLVAVFTVGYAVKTGLMSYQRLKELEISKQAWQDRADYYQISFGL
                     GDRGKDTENQSKWYAFAKEAIEEEQALYVKDNLLHFANPQGKNEQGETLDTYSPDANT
                     LYVSPSYLDKEKVVVDAETKQKLAHLQKGEFILLLPEHLRSREAELKKVFEERLSYYG
                     KSGEEASAPLDYEMKAHVSYLSMGEKRFVYNNGENPVSTQYLTDPILVVFTPTSTGDS
                     FISLSSWSINAGKQLFIKGYESGLELLKKAGIYEQVSYLKEGRSVYLTRYNEVQTETA
                     TLILGAIVGIASSLLLFYSVNLLYFEQFRRDILIKRISGLRFFETHAQYMVSQFASFV
                     FGASLFILSSRDLVIGLLTLLVFLASAVLTLYRQAQKESRVSMTIMKGK"
     gene            complement(8414..8713)
                     /locus_tag="VYN69_001594"
     CDS             complement(8414..8713)
                     /locus_tag="VYN69_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1533838.1"
                     /translation="MKTKKHRLLALALISSFTLLGAASAAVQYPDGGVWTYGEGSGGG
                     WAFSNYYHGKKYHYSSLVSRWNSHSDKGEAPAGKTSYAWIWTKWGEQVAFYCDYD"
BASE COUNT         2558 a         1965 c         1602 g         2731 t
ORIGIN      
        1 tattattaat tccagcagag gcgttttctt tctcaagctc actagttgta tccgaacccc
       61 aacgcggcaa cgttaaatca taatttccaa tctgtctagc taaaactgta gggagaaaca
      121 aactagatgc aattgtaact acgcaaaaaa ggaatatatt tttcttcttt tcatgacatc
      181 gtcctcttaa gaagtgataa acaagatagt tgattgatca atttgataca tattacattt
      241 tcattatatc aataaaagaa agcgttgtca attattttat ttaaaatatt gtcatatttt
      301 aaataaaatg gtaaaagaga ctcggtagcc tcgtctctct ttatcttact gacctaacct
      361 tcttgccaat tttcttggtc ttctttaaag cgttttagaa gatcaaggcc ctctggcgtg
      421 atgtaacctt cttcttgggc tagatggata agctcgctat agtttgaaag cgtcacaagt
      481 ttaacaccag catctgcaaa gttcttatct gcttttggca attggtagct gaaaatcgct
      541 acaactccaa gtacatctgc tccttctcgc ttggctgctg ctacagcttc aagaactgaa
      601 ccacccgttg aaataaggtc ttcaaccact accatttttt gaccttgagc tacgcgacct
      661 tcgatttgat taccagctcc gtggtctttt ggtttgctac ggatgtaggc aaaaggcaag
      721 tccatcttat cagcaataat ggctccgtgt ggaatccctg ctgttgcagt tcctgcaatc
      781 acttctactt caggaaaggc ttctttgata gcttccacaa aaccattttc aattagggta
      841 cgagtttctg gataggctag tgtcacacga ttatcagtgt aaatcggtga cttgatacca
      901 gatgcccaag tgaagggttc ctctggtttg aggtaaacgg cttggatttt caagaggtgg
      961 ctagcgatat ctttagcaag tgtcatggta ttctcctttt atttttctaa tctagttctt
     1021 taattccagt cctgtgtcca ttcatccttg atggtatgat aagctgcaac aggatcctca
     1081 gcttgggtaa tgggacgtcc cactacgata tagtcactgc cgatttgata ggcatcagca
     1141 ggtgtcatga ctcgtttttg atctccaact gcagcaccag ctggacgaat ccctggtgtc
     1201 agacagataa aatctggatt ggtagcctgc ttgatgactt gtacttcctg agccgagcaa
     1261 acaacaccat ccaagccagc ttcagctgtc ttcttggcat agtaaatcac agactcttgc
     1321 agactggttt ggatattttg aaactcctgc atctgggctt ctgacgttga tgtcagctgc
     1381 gttacagcga tcaatttagc ttgactccca agaccttcac gcgccgcctt catcatctct
     1441 acaccaccag ccgcatggac attagtcata tcgacaccaa gctgagacaa gaccttcatg
     1501 gctgacttga ctgtattagg aatgtcatga agtttgagat ccaaaaagac actatgaccc
     1561 aaacctttta agtaggacac aatctcaggc cccgctgcgt aataaagctc catccctacc
     1621 ttgagataaa ggctttcttc tgctggaaaa agagctaaaa attccttgac cgcctcaaaa
     1681 ctaggaaaat caagagcaat gattggacga tgttctcgca taggtttctc cttttacctt
     1741 tactagtgag agagtctggc caacagaagc cacgaaaaaa ggaacctgcc aacagcaagg
     1801 ttccacgaaa aatgggtagt ctccaccctt tcaccgtcta accttagctg cctctctgga
     1861 ctgctttaaa ggttttttac tattctatta tttctactag cacttgtcaa gtaaaaacat
     1921 ggtcactaaa gaactataag agaaaaagta aacctagcga cgcgatgagc gctgggtcgt
     1981 ttggtttcga ttgctctctt cctcttgttt tttctgttct tcttcttgtt ttttctcagc
     2041 ttccttagcc tcttgtttgg ctttttcctc agcttccata attaatttat ccgccacagt
     2101 gtagctgtag attccagctt ccatgtcgac cacactcggt tctgacaatt gaggcttaat
     2161 cttactgtaa tatggcagtt tcttactcat ttcagataga ggaaccaaga cttcgtccga
     2221 atcattcatg gtcaatcgaa ttaaatcgga tgtcaccttg cttggagcta attccacctt
     2281 ttggatagtc gccttgagtt ctgggctaat ttgagcaagt tctgagacaa aaaccttgat
     2341 ttgttcacta tcattaaaga gaactgataa ataagtttct ggtaaactgt tcagactcac
     2401 agaactagtc tcaagctgac cactggaaag aataggataa tgattttcac cagaaatata
     2461 gtaggccaca atatcatatt ccttgacctt aatagtgaac ttagttggaa attgatagac
     2521 aagttgagct gattcaaccc aatagttaga cttaatctgc ttttcatatt ttgccttgtc
     2581 tagcagaagg ttaatcgtat aatccgaatc ctgaatgcct gaagcctgtc gaatatcatc
     2641 agctgtagtt tgcaccgttc cctcaacacg aatatctttc atggtcgcat aaggactgag
     2701 caagtaggca gagacaatca ataaaagcag acttggaaat aaaatcgtga aggctcgcaa
     2761 gatatggata ccaggaatct ttgctttggc tggtttttcc tttgtagcct ttttagcaag
     2821 ctttttatcc tgttcctcct tctctttaga ctctgattct tctttctctt ctttctcttt
     2881 gtcagcctct gaggatgcta ctttttcttc agactcttcc ttagctgatt ctgaatcttc
     2941 ctggtctgtt tcactctcct ggtcctgttt gtcctctgac ttctcagatt cttctcccat
     3001 ccgagcttgt ctttcctttt ccttctcctc agctagagcc gcttcttctt cagccttctt
     3061 ttttagatat tcttggtttc gtttctgcca ttctgataac tctttcaatt cttcgaggat
     3121 ttctttgtcc tcatttttct tatcttttga catttacttt ccttatgata aatctttttt
     3181 caacaattga taaaaatctg ctagagattt caattcctta gaagctttca tcttagcttg
     3241 gtaatcttcc ttgtgactta gtaagtgaga aagcttctct tccaaactat ccaaggtcaa
     3301 atcgctttct tgaaggtctt ctgcatagcc tttcttaaca aagtaagctg cattttcaat
     3361 ctggtcacca cgactagctt cacgaccaag tggcacaatg acatgcaatt ttgctatcgc
     3421 caagagctca aaaatcgtat tggcaccacc acgtgtcaca acaatatcag ccaattccat
     3481 caagggttga tagagatcgg tcacatagtc aacacgaaaa agattttggc tcaactcatt
     3541 caggctagaa tctccagtta gattgataat attgtagcgc tctgttagtt ctttcttatg
     3601 gtctgtcacc aattggttaa agacacgagc acctgcagaa ccgccaacaa acaatacagt
     3661 cggcaattta tgattaaagt ggctttgaat atccaccaat tcatctggtt ctggattttt
     3721 ttgatctgaa accttggtca ctgctcccac atgctcaacc ttagacaaac tcgaagcttg
     3781 ctcaaaggtt gaatacatct tagtcgcaaa tttataggcg attttattgg ccaagcccat
     3841 agacaggtca gattcgtgaa taaagacagg cactcctgac acacgcgcag cgataacagg
     3901 cggtactgag acaaagcccc cctttgaaaa aagggtctgt ggacgaagtc gcaacatgat
     3961 aaagagcgat tggacgattc cccagccaac tttgaagacg tccagcatat tttgccaaga
     4021 gaaatagcga cgcaatttcc cagtcgcaat ggagtggaag gtgacatcca aacctgactt
     4081 aaggatttct tggtgttcga taccacgctt gtccccgata tagtggactt cccaaccatc
     4141 ttcgatgaac ttaggcatta acaaaagatt gagggtaacg tgtccaaccg tccccccacc
     4201 tgtaaagaca atttttttca tattattctt ttaactccgc tactgtgtcg ataaagaggt
     4261 cgccacgtac ttcaaagtta gcatacatat cccagctggc attggcagga ctaagaagaa
     4321 ccacatctcc ttgagtcgca atctcatagg ccttgcgggt cgcatctgca atatctgtcg
     4381 cctccacata agcgacacca gccttgtctg ctgcccgttt gacacgttct gcagattgac
     4441 ccaggatgac catcttcttg agtccaggaa tgtctggcac caattcgtca aactcattgc
     4501 cacggtccaa accacctgca atcaagacga ccttgctgtt gtcaaatcct gacaaggctt
     4561 tttgagtagc caagatatta gttgatttac tgtcgttata gaatttaaca cccttgatgt
     4621 catccacaaa ctggagacgg tgtttgacac caccgaaggc tgaaagagtt tccttgatgg
     4681 tttgattgtc cacaccacga agcttggcta cagcaatagt cgcaagggca ttttccacat
     4741 tgtggctacc tggaacaccg atttcattcg ctgccatgac tacttcacca cggaagtaga
     4801 gttgaccatc ttccagataa gctccatcaa ccttttcaag tgttgaaaat ggtacaacag
     4861 tggcttctgt cttggaagtc aagtcttttg ccaagtcttg attaaagttc aagacaagga
     4921 aatcagctgc tgtcatcttg ttctggatat tccacttggc tgctacatat tccgaaaatg
     4981 acccatggta gtcgatatga gttggcatga ggttggtaat aaccgcaatc tctggatgaa
     5041 attcttgaac acccatgagt tggaaagaag aaagttccat aacaagcgtg tccttgtccg
     5101 acgcagtttg ggccacttga ctagctggat agccgatatt ccctgataaa agaccatgtt
     5161 gcccagcagc agtcaaaact tccccaatca tagtcgttgt ggttgtctta ccgttcgatc
     5221 ctgtgatacc aataatcggt gcttctgaaa tcaaataagc caattccacc tcagtcaaga
     5281 ctggaattcc cttggccaaa gccttttcaa tcatgggatt gttgtagggg atacctggat
     5341 ttttcaccat aagggcaaac tcttcatcca agagttccaa aggatggcca cctgtaatga
     5401 ccttgatccc ttcttccagc aaactttggg cagctggatt gtcctcgaaa ggtttcccat
     5461 catttactgt cacaatggca cctagcttgt ccaacaaacg agctgcagat tcaccagact
     5521 tggccaaacc taaaacaagg actttcttat ttttaaattg atctattact ttcatgtctc
     5581 gaactccatt tctactccta ctattttacc atttttatgg aaataaaaaa gccacaaagt
     5641 gtgtttgtga ctctttcttc taactgaatc ttaccatatc atctatgtga taaatcggta
     5701 actcgaatga cctgatccac ttgctcccaa atcagaggat tgtgggtcgc aataataatg
     5761 gtccgattcg gattttttag agattctagg atggaaagta attcctcaga gtttttgggg
     5821 tctagtgaag cggttggttc atctgcgaga atcaaaggtg gatcctttaa aattatcttc
     5881 gctaatgcaa cacgttgtgc ttctcctcct gataactcaa atataggttg cttcaaatcc
     5941 aaataagaga ggtttacacg gtttagagct tgtttcatca aagagatttt ctctttttcc
     6001 ttcaactttt taccaactaa acccagattg agattctctt tgacggtttg gttttcaatt
     6061 aagccaaaat cttgaaataa atatcccaag taatctctaa agaaaacaga aggcttgatg
     6121 tccttaagag aagtaccatc atagatgatt tgtcctttgt catatggctc taatcgtcca
     6181 atcatattca agaatgttgt cttaccacag ccacttgtac cgattaaggc ataaattttc
     6241 ccaccttcaa aatgaagatt tgtatctgaa aatagttgac ggcttccaaa ttttttagat
     6301 atattcttta gttcaatcat cctattttcc tttcataatt gtcatagaaa cacgagattc
     6361 tttctgcgct tgacggtaaa gcgtcagaac tgcactagct agaaagacta ataaagtgag
     6421 caagccaatc accaagtctc gactgcttaa aataaagaga ctagcaccaa atacaaaact
     6481 ggcaaattgg ctaaccatat actgagcatg tgtttcaaaa aatcgtaaac ctgaaattcg
     6541 tttaatcaag atatctcggc ggaattgctc gaaatataga agattgacag aataaaatag
     6601 taacaaggaa ctagctatcc ccacaatagc tcctaagatt aaagttgctg tttcagtttg
     6661 aacttcatta taacgagtta gataaacact tcttccttct ttaagatagg atacttgctc
     6721 ataaattcca gctttcttca agagttctag cccactctca tatcctttga taaagagttg
     6781 ttttccagca ttgatagacc aactagataa ggatataaaa ctatcacctg tagaagtcgg
     6841 cgtgaataca actaaaatcg gatcagtcaa atactgagta gatacgggat tctcaccgtt
     6901 attataaaca aaccgctttt ctcccattga aagataacta acgtgcgctt tcatctcata
     6961 atccaaagga gcacttgcct cctcaccaga ttttccataa taactcaatc tttcttcaaa
     7021 aactttctta agttctgctt ctcgagagcg caaatgttct gggagcaaga ggataaactc
     7081 acctttttgg agatgggcta acttctgttt ggtctcagca tctaccacga ccttttcctt
     7141 gtccaaataa ctgggactaa catagagcgt attagcatct ggactatagg tatccagtgt
     7201 ctctccctgt tcattttttc cttgtggatt ggcaaaatgg agcagattat cctttacata
     7261 aagagcttgt tcttcttcga ttgcttcctt ggcaaaggca taccacttgc tctgattttc
     7321 tgtatctttt cctctatcac ctaagccaaa agaaatttga taatagtctg ctctatcctg
     7381 ccatgcttgt tttgaaattt caagttcttt caatcgttgg taagacatca aacctgtctt
     7441 aacagcgtag cctactgtaa agacagcaac caactgacac aatagggtta aaatcatcag
     7501 gcgtttaagg ggtaatctcc ccttaataac gggaactaat gctttgtaac tcaaactcat
     7561 tagataaaga agcattagta aaattgaaat cgccaataaa aacaacagat agaaactaat
     7621 cccaaaacca taggtggcta acaagatagg ataaaacaaa ccttgactaa aaagaacgac
     7681 tcccccacct aggaaggaaa ggagggctga tagaaggagc catttgatat cagtagataa
     7741 agaatgcccc atgatggata agagagtctg accagaaaag agttttatac ctgctgctct
     7801 catttcctta atccgagtga taatcactaa agcaaagaaa gataagccaa atattgctaa
     7861 actaattaaa ataaggggat ttagtaatat tcgaaaagca agaaaatagg gcggtatctt
     7921 tcggtcagca cttgctttat aacccaaatc tcctaattta tcggcaagct tttctttcgt
     7981 caaggagcct gacaaaagga gataactatt tagcggatca ctacgttcac gactttcttg
     8041 gctagcttct tggaattctt ttggtaaagt tccctgacca taagttgcat aagtaaagtg
     8101 agtcgtccca tccttactcg gctctacaat tcttctagct attaaactct gttctgagtt
     8161 tgcaaaattc tccaattcct gttcaaatac ctcacgcgtc ggttcctgag tatctttttt
     8221 gacacgaagt aaagaaacgg aatcatagct tgcatataaa tattgtggcg cacgtaagac
     8281 aataatccaa gcaaggaaga agctgagaaa aaaagttgat aataatatga atagtttctt
     8341 catagtagac tccttgtaaa caaaattccc cctgtaattt cttacaaggg gaacgattta
     8401 aatcaatgaa cgattagtca taatcacagt aaaatgctac ttgttctccc catttagtcc
     8461 aaatccatgc ataggaggtt tttccagcag gagcttctcc tttatctgaa tgactattcc
     8521 atctacttac aagagaagaa taatgatatt ttttaccatg atagtaattt gaaaaagccc
     8581 aaccacctcc tgaaccttct ccatacgtcc atactcctcc atctggatat tgtacagcag
     8641 ctgatgcagc tcccaataat gtaaaacttg aaataagagc tagagcaagt aatctatgtt
     8701 ttttcgtttt cattttattt tttctttcaa aaaaatcaca ccttgagcaa caatgcaaca
     8761 aaataagtcc tcctctctct tttattgaaa ccgctttctt atgtgataag aataacattt
     8821 ttattatttg ttgtcaagga aaaaatcgaa tttttt
//
LOCUS       DAVLVP010000073         8839 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.073, whole genome shotgun sequence.
ACCESSION   DAVLVP010000073 DAVLVP010000000
VERSION     DAVLVP010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8839
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.073"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            172..243
                     /locus_tag="VYN69_001595"
     tRNA            172..243
                     /locus_tag="VYN69_001595"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:205..207,aa:Glu,seq:ttc)
     gene            249..322
                     /locus_tag="VYN69_001596"
     tRNA            249..322
                     /locus_tag="VYN69_001596"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:283..285,aa:Asn,seq:gtt)
     gene            complement(363..905)
                     /locus_tag="VYN69_001597"
     CDS             complement(363..905)
                     /locus_tag="VYN69_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEV1533839.1"
                     /translation="MQESNKRLKTKRTIENAMVQLLMEQPFDKISTVKLVEKAGISRS
                     SFYTHYKDKYDMIEHYQSKLFHTFEYIFQKHAHHKRDAILEVFEYLESEPLLAALLSE
                     NGTKEIQNFLRNKLHIMLSTDLQKRFMQLNLNTTELEYSSIYLTHALFGVCQTWIAHG
                     KKESSQEITDFLMKMLGDTN"
     gene            complement(1165..2418)
                     /locus_tag="VYN69_001598"
     CDS             complement(1165..2418)
                     /locus_tag="VYN69_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEV1533840.1"
                     /translation="MKCFVGLDISSTKLDVCIMLSDTSTPVTASLSNDLSGATEIKNH
                     ILDLNHTYHFERIVIGMEATSLYSFHPAMFFHEDRQLKELHVEIMVEQPNKIKKYRDA
                     FEENKNDTIDAFYIADYFRIERFSPSFLKEEKYMALQHLTRTRLQIIEQLTRTKQHFI
                     ENIYYKCNTLAYQFKKEELSTNLWSSTMMTLMTDEMTLDELANMPLQELDDWLQKLGK
                     GHFKDSEKLAQTIQAAIRGSYRLSKLQQDSVNVILGLLAREIRNLEKMIKEIDKAIED
                     MVETIPEYQCLTSIPGVGKVYAAGIIAEIGQIERFKDHPQVAKFAGLNWREKQSGNSN
                     SQHTSLVNRGNRYLRYYLVEAANSVRRYDDEYKDFYKKKYHEVPKHQHKRAILLTARK
                     FVRLVDVLLRNHQLYTPPRRIMEDN"
     gene            2774..4532
                     /locus_tag="VYN69_001599"
                     /pseudo
     CDS             2774..4532
                     /locus_tag="VYN69_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007518194.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
     gene            complement(4562..7105)
                     /locus_tag="VYN69_001600"
     CDS             complement(4562..7105)
                     /locus_tag="VYN69_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEV1533841.1"
                     /translation="MKSFLKTYRTYFISFIIPVVIMSGVYLSQGIYWNSDNSPLLGDG
                     FHQYVIFDVALRNILHGNSSLFYTFTSGLGLNFYALSSYYLGSFLSPLVYFFDLTNMP
                     DAVYLTTLLKFGLIGLSTFFSLNKLFQSIPQTLKLALSTSYTLMSFTVSQLEIKTWLD
                     VFILIPLIITGLHLLITEKKLLLYFTNLSILFIQNYYFGYMTVLFLIFWYLCQISWDF
                     KTRKSSVLDFIVISFLAGMASLIMTLPTLFDLQTHGEKLTEVTKFQTESSWYLDLFAK
                     QFIGSFDTTKYGAIPMIFVGLLPFILTILFFTLKSIKFHVKLIYVLFFAFLIASFYIE
                     ALDLFWQGMHTPNMFLHRYAWIFSTLLIYTAAEVLKRLKELKVWNFLVSLFLVVTGFL
                     ATIYLKSHYSFLTDLNILLTLEFLVVYSLLLLAVIKKFISVNLFAILISLFILVEMSL
                     NASSQMDGIAKEWGFASRSTYSRDIPAMESFSTYIGNQFTRTEKLQTQTGNDSMKFNY
                     NGISQFSSVRNRSSSSTLDKLGFKSSGTNLNLRYANNSILADSLFGIQYNISDSPIDK
                     YGFKDIYQKDNLTLYENQYSLPIAVASQSVYNDVKFNEHTLDNQASFLNQLANVDFDY
                     FSPIPYEKIENTNNLISVTSSSNEDAAIQYQIEVPENSQIYLSFINLHFSNDKQKKVD
                     ILVNGEKKTFTTDNVFSFFNLGYTKEKKTFNINVSFPGNSQVSFESPTFYRLDTKTFT
                     EAIQKIKEQPVTVSTSKNKVFATYDVQQDTSIFFTIPYDKGWSAYQDGKKIEIKQAQT
                     GFMKVDIPKGKGTITLSFIPNGFITGAICSFTSLLLFGIYNHRRKSSKA"
     gene            complement(7167..8789)
                     /locus_tag="VYN69_001601"
     CDS             complement(7167..8789)
                     /locus_tag="VYN69_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015511338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1533842.1"
                     /translation="MLTVSDVSLRFSDRKLFDDVNIKFTEGNTYGLIGANGAGKSTFL
                     KILAGDIEPTTGHISLGPDERLSVLRQNHFDYEDERAIDVVIMGNEKLYSIMKEKDAI
                     YMKEDFSDEDGVRAAELEGEFAELGGWEAESEASQLLQNLNIPEELHYQNMSELANGE
                     KVKVLLAKALFGKPDVLLLDEPTNGLDIQSITWLEDFLIDFDNTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWKESSELAAKLLADRNAKAEEKIKQLQEFVARFSANA
                     SKSRQATSRKKMLDKIELEEIVPSSRKYPFINFKAEREIGNDLLTVENLTVKIDGETI
                     LDNISFILRPDDKTALIGQNDIQTTALIRAIMGDIDYEGTVKWGVTTSQSYLPKDNSA
                     DFAGGESILDWLRQFASKEEDDNTFLRGFLGRMLFSGDEVNKPVNVLSGGEKVRVMLS
                     KLMLLKSNVLVLDDPTNHLDLESISSLNDGLKNFKESIIFASHDHEFIQTLANHIIVL
                     SKNGVIDRIDETYDEFLENAEVQAKVKELWKD"
BASE COUNT         2840 a         1501 c         1682 g         2816 t
ORIGIN      
        1 atattcagtt ggatcctttt catatgacta ttctaccaaa tgggacattt tcacgttcga
       61 tttactaaag acattatcac attcgaatta catattttag agaaaaaatc tcaaaagtga
      121 ttgacaatta gcaagaaatt gatataatgg ttatatctgc tacagataga aggtccgttg
      181 gtcaaggggt taagacaccg ccttttcacg gcggtaacac gggttcgaat cccgtacgga
      241 ctattttatc cgccatagct cagttggtag tagcgcatga ctgttaatca tgatgtcgta
      301 ggttcgagtc ctactggcgg agtatagata aaagggaaca cagctgtgtt cctctttttg
      361 tatcaatttg tatcaccaag cattttcata aggaagtctg ttatttcttg agaactttct
      421 ttttttccat gtgcaatcca agtttggcag acaccaaaaa gtgcatgagt tagatagatg
      481 ctactatatt ctaattcagt ggtatttaga ttcagttgca taaatcgctt ttgtaaatct
      541 gtactaagca tgatatgaag tttatttcgt aagaaatttt ggatttcttt agtcccattt
      601 tcagaaagaa gggcagccag aagtggttct gattctagat attcaaaaac ttctaaaata
      661 gcgtctcttt tgtgatgagc atgtttttga aaaatatatt caaatgtatg gaatagcttg
      721 ctttgatagt gctcaatcat atcatactta tccttatagt gagtatagaa gctggaacga
      781 ctaattccgg ctttttctac taatttgaca gtagaaattt tatcaaatgg ctgttccatc
      841 agtaattgta ccatagcgtt ttcaatagtt cgctttgttt ttaagcgttt gttactttct
      901 tgcatatttc ctccttgtaa acaaattaga ttatatgtct aaaaatagat tttttatctt
      961 gtaatttaga ttttttaatg tataatctat tatatcaaaa ttttagacaa tatgtttaaa
     1021 aaagtctagt ggtaaaatgt caagcgaccc cccgctcaaa gtttgaaaac tatttcaaaa
     1081 aaagggtgca ctaaagaagc cctactgaaa acgatgtttt cacactgttt ttagtagatt
     1141 ttcaaggaga ggtgcagata taacttagtt atcttccata atcctccttg gtggcgtata
     1201 gagttgatgg ttgcgtagta gcacatccac cagacgcaca aattttctag cggttagtaa
     1261 gatggctcgt ttgtgttggt gtttaggtac ttcatggtat ttcttcttat agaaatcctt
     1321 gtattcgtca tcataacgcc ttaccgagtt ggcggcttca accaggtagt agcgcagata
     1381 acgattgccc ctattcacaa gcgaagtatg ttgtgagttt gagttaccag attgcttttc
     1441 tctccaattc agaccagcaa attttgcgac ctggggatgg tctttaaagc gttcgatttg
     1501 tccaatttcg gcaatgatac cagcagcgta gaccttcccg accccaggta tggaggtcag
     1561 gcattgatat tcaggaatgg tttccaccat atcttcaatg gccttatcaa tttccttgat
     1621 cattttttcc aggtttcgaa tttcccgagc cagaagtccg agaatgacat tgactgaatc
     1681 ttgttggagt ttagagaggc gataagaacc tctgatagcg gcttgaatgg tttgagcaag
     1741 cttctcagag tccttaaaat gccctttacc cagtttttgg agccaatcat ctaattcttg
     1801 aagaggcatg ttagcaagtt cgtctaaggt catctcatct gtcatcaagg tcatcatcgt
     1861 tgaactccat aggttagtag agagttcttc tttcttgaat tgataagcca aggtattgca
     1921 cttgtagtag atattttcaa tgaagtgctg tttggttctg gttaattgct cgatgatttg
     1981 gagtctggtt cttgtgagat gttggagagc catgtatttc tcttctttta aaaaagaagg
     2041 tgaaaatcgc tcaatacgga aataatccgc aatgtagaaa gcatcaatgg tatcgttttt
     2101 gttttcttcg aaagcatccc gatacttctt gattttatta ggttgttcga ccataatctc
     2161 aacatgtagt tctttcaact gcctatcttc atggaagaac atagcagggt ggaaactgta
     2221 gagactggtt gcttccatgc caatgacaat cctttcgaag tgataggtat gattcaaatc
     2281 aaggatatga ttcttaatct ctgttgcccc agaaaggtca ttagagaggg aagcagtgac
     2341 gggggtgcta gtatcactca acatgataca cacatctaat tttgtagagc tgatgtctaa
     2401 accgacaaaa catttcatta aggaaggtct cctttccatt agatttggaa gtttttttga
     2461 ctactgtaag gtttcccaga aacaccttgt tccaacatcc tcgcataaga gaatccttat
     2521 cacagcctga cagagccgcc tgcatcctac tagatacaga agtcaagctg tggaaacagc
     2581 tagtgtgttg gaagtttgaa caaggcttgg gtaacagact ttttaatgaa gtccaagcaa
     2641 caaggagaaa aagaaacaac catgagtcca ttccatgact ctattgttct agaaaacctt
     2701 gcaatagtca agtgataacg aataaaactt gggattgaaa tatccccatg attatatatt
     2761 acgaggagaa actatgttta aagaatggaa agcaatttaa aaaaaacaac ctttattatt
     2821 gtcatgatcg ggatttctct tattccagct ctgtacaata tcatattttt gtcatcaatg
     2881 tgggatccat atgggcaatt gtctgactta cctgtggcag ttgtaaataa tgataaagag
     2941 gcttcctata atggtaatac tatggcaata ggaaaagaca tggtgtccaa tttaaaagaa
     3001 aataaaacct tggattttca ttttgtagat gaagaggaag gaaagaaggg attggaagat
     3061 ggcgattact atatggtagt gactttacca agtgatttat ctgaaaaagc aactacatta
     3121 tccaatattc aatcgacagc agcttatcaa tcattgacaa gtgagcaaca aactgagata
     3181 agtgattctg tatctcaaaa ttcaactgat agtattcaat cggctcagtc aattgtagct
     3241 ttagtacaag atttacaggg aagtttagaa aacttacaaa atcaatcttc taatctttcg
     3301 actttaaaaa atcaagctaa tcaagtatta cctattactt ctacttcttt gataggattg
     3361 tcaagtggat taacagagat acaaggagct gttactagca aattagttcc tgccagtcag
     3421 tcgattgcat caggtgtaaa cgcatatact acaggtgttg ataaagtttc tcagggcgca
     3481 agtcaactaa gtgaaaaaaa tgctaccttg acaggtagtt tggatcaatt agtttcaggc
     3541 tcaaacacct tgacacaaaa atcttctagc ttgacagcag gagttggtca attagttgaa
     3601 aaaactccag aattagtatc tggtattgaa aaattatcaa ctggctccaa ttaattgaat
     3661 caaaagagtc aagaattgat agcaggagtt gataaattgc agtcaggctc tagccaacta
     3721 gctgacaaat ccagtcagtt actttcaggt gtttctcaat tagaaagtgg agctaataaa
     3781 ttggcagatg gagctgggaa actagcagaa ggtggaacaa agttaacttc tggattggaa
     3841 ggtttacaga caggacttgc ttctttagga caaggactag gtaatgctag tgatcaactc
     3901 aaatcagcat caacagaatc taaaaatgca gagattttgt caaatccact caatctttcc
     3961 aaaacagaca atgaccaagt tcctgtaaat ggaattgcaa tggctcctta tatgatatca
     4021 gttgctcttt ttgttgcagc aatatcaacg aatatgatct ttgcgaagtt accttcagga
     4081 cgtcatccag agagccgttg ggcttggttg aaatctcgag ctgaaataaa tggtattata
     4141 gctgttttgg cagggatttt ggtatatgga ggagttcatc ttattggtct aactgctaat
     4201 catgaaatga gaacatttat tctcatcatc ttaacaagtt tagtattcat gtctatggtg
     4261 accgctttaa caacatggaa tagccgtata ggagctttct tctcgcttat ttttctttta
     4321 ttacagttag catcaagtgc aggtacctat ccgcttgctt tgacaaatga tttctttaga
     4381 gctattaatc cttggttgcc aatgagttat tcagtttcgg gattacgaca aacaatctcc
     4441 atcaacaagt cattttccta gctgtcatac tagctctatt tactggttta ggtatgctag
     4501 cctatcaacc taagaaaatg gaagaagatt aaaaaaatcg accgattaac tggtcgattt
     4561 tttatgcctt agatgacttt cgtctgtgat tatagattcc aaatagtaag agagaagtaa
     4621 aggaacagat tgctccagta ataaaaccat tgggaatgaa ggaaagtgta atagttcctt
     4681 tccccttggg aatgtcaact ttcataaatc cagtttgagc ttgtttaatt tctattttct
     4741 taccatcttg gtaggcagac caacctttgt cataaggaat ggtgaagaaa atagatgtat
     4801 cttgttggac atcatatgta gcaaaaacct tgtttttaga agttgatact gtgacaggtt
     4861 gttctttaat tttttgaatt gcctcggtga aagttttggt atctaaacga tagaaggtag
     4921 gagattcaaa tgatacttgt gaatttccag ggaaactaac attgatattg aaagtttttt
     4981 tctctttagt atatcctaga ttaaagaagg agaagacatt atcagttgta aaagtctttt
     5041 tttcaccatt tacaaggatg tcaaccttct tttgtttatc gttagaaaag tgaaggttta
     5101 tgaaagagag ataaatttgg ctgttttctg gaacttcaat ttgatactgg attgctgcat
     5161 cctcatttga agaacttgtg acactaatca aattattagt attttctatt ttttcataag
     5221 ggattggaga aaaataatca aaatcaacgt tagcaagttg atttaaaaac gaggcctgat
     5281 tatccaaggt atgttcattg aacttgacat cattgtaaac agattgacta gcaactgcaa
     5341 tcggaagaga gtattgattt tcatataggg taagattatc tttttgatag atatctttaa
     5401 aaccatactt atcaatagga ctgtctgaga tattgtactg gataccaaat aaactatcag
     5461 ccaaaatact attatttgca tatcggagat tgagattagt cccagaggat ttaaacccaa
     5521 gtttatctaa agtagagctt gatgaacgat ttcgaacaga tgaaaattga gagattccat
     5581 tgtagttgaa tttcatactg tcatttcctg tctgagtttg tagtttttca gtacgagtaa
     5641 attgatttcc aatatatgtt gagaaagatt ccatagctgg gatatctcga ctataagtac
     5701 ttcgagaagc aaatccccat tccttagcaa ttccgtccat ttgagatgaa gcatttaaac
     5761 tcatttcaac cagtataaat aaagagatta gaatggcaaa tagattcaca gatataaact
     5821 ttttgataac tgcaaggagt aaaagagaat agacaaccaa aaattcaaga gtaagcagaa
     5881 tattcaaatc tgttaaaaaa gaataatgcg attttagata gatggtagct aaaaatcctg
     5941 ttactacaag aaaaagcgaa actaaaaaat tccagacttt aagttctttc agacgcttta
     6001 agacttctgc tgctgtgtaa attaacaagg tagagaaaat ccaagcatag cgatgtaaaa
     6061 acatgtttgg agtatgcatg ccttgccaaa ataagtcaag agcttctatg taaaagcttg
     6121 caattagaaa tgcaaagaat aatacatata tgagtttcac gtgaaactta atagatttca
     6181 gcgtaaaaaa taaaatggtc aaaataaaag gaagtagtcc aacaaaaatc attgggatgg
     6241 ccccatactt tgttgtgtca aaggaaccaa tgaattgctt agcaaagaga tcaagatacc
     6301 agctactttc agtttgaaac tttgtaactt cagtcaattt ttccccatgt gtctgtaaat
     6361 caaacagagt gggaagagtc ataatcaaac tagccatacc agctaaaaag gagataacta
     6421 tgaaatcaag aacagatgat tttcgagtct taaagtccca cgaaatttga cagagatacc
     6481 agaaaataag aaacaatact gtcatatatc caaaataata attttgaata aataagattg
     6541 acagatttgt aaagtacaat aggagtttct tttcagttat cagtagatgt aaaccagtta
     6601 taattaaagg aatcaagata aaaacatcta gccaggtttt tatctctaat tgactgacag
     6661 tgaaactcat cagagtatag gaagtagata aggctagttt taaagtctga gggatagatt
     6721 gaaacaattt attcaaacta aaaaaggttg acagaccaat caatccaaat tttaagagag
     6781 tggtcagata aacagcatct ggcatattcg ttagatcaaa aaagtaaacc agaggtgaga
     6841 gaaaactacc caagtaataa ctagacagag catagaagtt tagccctaga ccacttgtaa
     6901 aggtgtaaaa cagactacta tttccatgta ggatatttcg taaggctaca tcaaaaataa
     6961 cgtattgatg aaagccatct cctaatagag gagagttgtc gctattccag tagatacctt
     7021 gagatagata tactccagac ataatcacta caggaatgat gaaagaaata aaataggttc
     7081 gatatgtttt taaaaatgat ttcatgttac cttgtagaat gatagaaaac tcagttggtt
     7141 aacccaactg agttttgaag ttttatttag tctttccaaa gttctttaac ttttgcttgt
     7201 acttctgcat tttctaggaa ttcatcgtag gtttcatcga tacggtcaat gacgccattt
     7261 ttagataaga caatgatatg gttagccaaa gtttgaataa attcgtggtc atggctggca
     7321 aagatgattg attctttaaa gtttttcaat ccatcattca agcttgagat agattccaag
     7381 tccaagtgat ttgttggatc atcaagtaca aggacatttg attttaagag catgagtttt
     7441 gaaagcatga cacgaacttt ttctccccct gacaagacat ttacaggttt gttaacttca
     7501 tctccagaga agagcatacg gccgaggaag ccacgtagga aagtattgtc atcttcttct
     7561 ttacttgcga attgacgcaa ccagtcaaga attgattctc ctcctgcaaa atcagctgag
     7621 ttatcttttg gtaggtaaga ttgactagtt gtaactcccc acttgacagt tccttcatag
     7681 tcaatatctc ccatgattgc acgaattaat gcagttgttt gaatatcatt ttgtccaata
     7741 agtgctgtct tatcatctgg acgcaagatg aaactaatat tatccaagat agtttcacca
     7801 tcaatcttta cagttagatt ttctactgtc aagagatcat taccaatctc acgttccgct
     7861 ttaaagttga taaatggata tttacgacta gatggtacaa tctcttctag ctcaatctta
     7921 tcaagcattt tcttacgtga tgttgcctgc cttgacttag aagcattggc agagaaacga
     7981 gcaacgaatt cttgcaattg tttaattttt tcttctgctt tagcattacg gtctgctagc
     8041 aatttagcag caagctcaga agattccttc cagaagtcgt agtttccgac atagagtttg
     8101 atttttccaa agtcaaggtc ggccatgtga gtacaaactt tgtttaagaa gtgacggtcg
     8161 tgggatacta cgataactgt gttatcaaag tcaattaaga agtcttctaa ccaagtaatc
     8221 gattggatat ccaaaccgtt agtaggctcg tccaagagaa gaacatctgg tttaccaaaa
     8281 agtgctttgg cgaggagaac ctttactttt tcaccgttgg ccaattcgct catgttttgg
     8341 tagtgtaatt cttctggaat gtttaggttt tgaagtagtt gagaggcttc actctctgct
     8401 tcccaacctc caagttcggc aaactctcct tcgagttcgg cagcacgaac cccgtcctcg
     8461 tctgagaaat cttccttcat gtagatagca tctttctctt tcatgatgct ataaagtttt
     8521 tcatttccca tgataacgac atcaatggca cgttcatctt cgtagtcaaa gtgattttga
     8581 cgaagaacag agagacgttc atctggacca agagagatgt gaccagtagt aggttcgata
     8641 tctccagcta aaatttttaa aaaggttgat tttccggcac cattagcacc gattaatccg
     8701 taagtatttc cttctgtaaa tttgatattg acatcatcaa aaagtttgcg atcactaaaa
     8761 cgtagtgaaa catcagatac tgtaagcaat atttttctcc tatatgtgta atatatttat
     8821 tctactagaa aatacagaa
//
LOCUS       DAVLVP010000074         8697 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.074, whole genome shotgun sequence.
ACCESSION   DAVLVP010000074 DAVLVP010000000
VERSION     DAVLVP010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.074"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            17..232
                     /locus_tag="VYN69_001602"
     CDS             17..232
                     /locus_tag="VYN69_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000335289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1533843.1"
                     /translation="MCKQICYTLLEDAKNKKSYDRLAICYVRIGICTDDSKLIQKGFS
                     LLELTEETSMLSHLKKEVETHYQPKKL"
     gene            complement(521..1912)
                     /gene="argH"
                     /locus_tag="VYN69_001603"
     CDS             complement(521..1912)
                     /gene="argH"
                     /locus_tag="VYN69_001603"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001107608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1533844.1"
                     /translation="MAKNTKLWGGRFEGTVEDWVERFGASISFDQKLAKFDLIGSLAH
                     VQMLGQTGILSLEESEKIQAGLKELLEELEAGQLDFDIANEDIHMNMEVLLTEKIGPL
                     AGKLHTARSRNDQVATDMHLYLKEQLGHVLDKLVHLKGVLLDLAENHVETIMPGYTHL
                     QHAQPISFAHHLMAYYNMFQRDSERFEFNQKHTDLCPLGAAALAGTTFPIDRQLSSDL
                     LEFKKPYTNSLDAVSDRDFILEFLSNASILMMHMSRFCEEMINWCSFEYQFITLSDTF
                     TTGSSIMPQKKNPDMAELIRGKTGRVYGNLFGLLTVMKSLPLAYNKDLQEDKEGMFDT
                     VETILNSLDVLAGMLSSLQVNKEKMQESTEKDFSNATELADYLAGKGLPFREAHEVVG
                     RLVLDSIKSAKNLQDWTLEELQTYHSLITEDIYVYLQPKTAVQRRNSLGGTGFDQVEY
                     QIAVAKKANEAKK"
     gene            complement(1941..3137)
                     /locus_tag="VYN69_001604"
     CDS             complement(1941..3137)
                     /locus_tag="VYN69_001604"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HEV1533845.1"
                     /translation="MSKEKVILAYSGGLDTSVAITWLKKDYDVVAVCMDVGEGKDLDF
                     IHDKALKVGAVESYVIDVKDEFATDYVLVALQSHAYYEQKYPLVSALSRPLISKKLVE
                     IAHQTGATTIAHGCTGKGNDQVRFEVSIAALDPNLKVIAPVREWKWSREEEIHYAKEN
                     GVPVPADLDNPYSVDQNLWGRANECGILENPWNQAPEEAFGITSSPEAAPDMPEYIDI
                     EFSEGVPVSLNGEVLKLADLIQKLNEIAGKHGVGRIDHVENRLVGIKSREIYECPGAV
                     TLLTAHKEIEDLTLVREVAHFKPIIENELSNLIYNALWFSPATQALIAYIKETQKVVN
                     GTAKVKLYKGSAQVVARKSPNSLYDENLATYTSADTFDQDAAVGFIKLWGLPTKVHSE
                     VQKSAK"
     gene            complement(3154..3960)
                     /locus_tag="VYN69_001605"
     CDS             complement(3154..3960)
                     /locus_tag="VYN69_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001038484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1533846.1"
                     /translation="MNKMKKVLMTMFGLVMLPLLFACSNNQSAGIEAIKSKGKLVVAL
                     NPDFAPFEYQKVVDGKNQIVGSDIELAKAIATELGVELELSPMSFDNVLASVQSGKAD
                     LAISGVSKTDERSKVFDFSTPYYTAKNKLIVKKSDLATYQSVNDLAQKKVGAQNGSIQ
                     ETMAKDLLQNSSLVSLPKNGNLITDLKSGQVDAVIFEEPVAKGFVENNPDLAIADLNF
                     EKEQDDSYAVAMKKDSKELKEAVDKTIQKLKKSGELDKLIEDAFKASIEK"
     gene            complement(4167..4808)
                     /locus_tag="VYN69_001606"
     CDS             complement(4167..4808)
                     /locus_tag="VYN69_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000571330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533847.1"
                     /translation="MIELENITKTIGGKVILDNLSLRIDQGDLVAIVGKSGSGKSTLL
                     NLLGLIDGDYSGRYEIFGQTNLAVNSAKSQTIIREHISYLFQNFALIDDETVEYNLML
                     ALKYVKLSKKDKLKKVEEILERVGLSATLHQKVSELSGGEQQRIAVARAILKPSQLIL
                     ADEPTGSLDPENRDLVLKFLLEMNREGKTVIIVTHDAYVAQQCHRIIELGEGK"
     gene            complement(4805..6913)
                     /locus_tag="VYN69_001607"
     CDS             complement(4805..6913)
                     /locus_tag="VYN69_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HEV1533848.1"
                     /translation="MKKISNFCMLLLLLCTTFFVFNVNYTREVVRIQEMGKTVDSLDL
                     YLKDINEPAASVLRFFEDVSKEYKVSIIKTDSGDEVVKSGVFDKDTFPYQEFGISSLD
                     FTTDGEGVYSNKEISNKLGTIPTFLKAKPIQLMTFQTYIKDTSRSLNGRYTITSTQEM
                     DKDRIVQKWSDFFKIDQATLLEPTYKSAVEVINRDLLLSAIVFVLAILLLVLVTVYQP
                     MMEMKRVGVQKLLGFQDRAVLADVVKGNLYLLLGGALVINLGVFFLLDYKPKDLFPML
                     WLSHFLLLQLYLFISWLTYLLIQKMTISSLLKGFSSFKFGLLFNYLMKIGTTILLTVL
                     LVGVGKSLEQENKELDYQKQWISQGNYLTLETFQLNDNLWQEQLAGSGKALDYFYRFY
                     QDLVEKTQAGYVQSSSLPVKNFVQSEQIQQYQLTDTVDVYYANRHFLKSKGFKLPDTG
                     IKKVILMPASTKGEEDKNQLLGKLIAFHSMKYEEQQKRTIEEMDVEIAYYEGDWSFFP
                     YSDKRKENLSNPIISLVNDSDMMWDEKASLSTTGLNNPIKIENTVQHQKEITELVEKL
                     SDGNYLKFSSIQAIQQEKVDSYRDAVRNFNLLFALFGLLSMMISYFLLVITFLLKRRD
                     IITKKFMGWKLVDRYRPLLVLLLLGYSLPLLVLIFFAHALLPLLLFAGFTCLDILFVL
                     GLASRMEKRSLVELLKGGIL"
     gene            complement(6965..7252)
                     /locus_tag="VYN69_001608"
     CDS             complement(6965..7252)
                     /locus_tag="VYN69_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001230165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HEV1533849.1"
                     /translation="MRKLVKIGVASLMVCGILATTNALAVWVEGGQWNYGVGWTGTFG
                     YSDYLHSTRYHTATVRHGGRTSKDYAKPEAWARASLTKIPPTGMEYFYGFE"
     gene            7678..7998
                     /locus_tag="VYN69_001609"
     CDS             7678..7998
                     /locus_tag="VYN69_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEV1533850.1"
                     /translation="MQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMCKQICYTL
                     LEDAKNKKSYDRLAICYVPIGICTDDSKLIQKGFSLLELTEETSMLSHLKKEVEIYYQ
                     AKER"
     gene            complement(8050..8637)
                     /locus_tag="VYN69_001610"
     CDS             complement(8050..8637)
                     /locus_tag="VYN69_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000843467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEV1533851.1"
                     /translation="MKSITKKIKATLAGVAALFAVFAPSFVSAQESSTYTVKEGDTLS
                     EIAETHNTTVEKLAENNHIDNIHLIYVDQELVIDGPVAPVATPAPATYAAPAAQDETV
                     SAPVAETPVVSETVVSTVSGSEAEAKEWIAQKESGGSYTATNGRYIGRYQLTDSYLNG
                     DYSAENQERVADAYVAGRYGSWTAAKNFWLNNGWY"
BASE COUNT         2622 a         1945 c         1463 g         2667 t
ORIGIN      
        1 caatcaagat aaaaacatgt gtaagcaaat ctgctacact ttactagagg acgccaagaa
       61 taagaaaagc tacgataggc tggctatctg ctatgtccgt attgggattt gtacagacga
      121 ttctaaactt atccaaaaag gtttctcact cctagagctg accgaggaaa cttctatgct
      181 gtctcatctc aaaaaagaag tagagaccca ttatcaacca aagaaattat aaaaaagtcg
      241 agggatttcc tcgactcttt acatattatt tgttcgttag ttcttaatac cagccattgt
      301 taaaccagaa gtttttagct ctgccaagcc acatagtgtg cttactacta tgtgtccatg
      361 tcaccatgaa tcgccgaacg atttaacgag aaagtatgac ttttacgttt atcccaactc
      421 aattatgaca tttttttcaa aagtcaatat atctcacttt ttcaacgaca agaaagaggc
      481 tgccaatcga ccaacctctt attctgaacc catcactcca tcacttttta gcttcattcg
      541 ctttcttagc gactgcaatc tggtattcga cttggtcaaa tcctgtgcct cccaaggaat
      601 ttcgtctttg aacagcagtt ttaggttgca agtagacata gatatcctcg gtaatgagag
      661 aatgataggt ttgtaattcc tccaaagtcc aatcttgaag atttttagca gacttgatag
      721 agtctagcac taatcttccc acaacctcat gagcttctct aaatggcaag cctttccctg
      781 ccaaataatc tgccaactca gtcgcatttg aaaagtcctt ctctgtagac tcttgcattt
      841 tttccttgtt tacctgcaag ctggataaca tacctgccaa tacatcgaga gaatttaaaa
      901 tcgtttcgac cgtgtcaaac attccttcct tatcctcttg caaatcctta ttataagcta
      961 agggcaagga cttcatgact gtcaacagtc caaacagatt cccataaact ctaccagtct
     1021 ttcctcgaat caactcagcc atatctggat ttttcttttg gggcataata gaggaacctg
     1081 ttgtaaacgt atctgacaag gtaatgaatt ggtactcaaa actacaccaa ttgatcattt
     1141 cttcgcaaaa acggctcatg tgcatcatga gtatgctggc atttgacaga aattctaaga
     1201 taaaatcgcg gtcactaaca gcatccaaag aatttgtata aggtttctta aactccagca
     1261 aatcgctaga caattggcga tcaatgggga aagtcgttcc tgccaaagct gccgcaccta
     1321 gagggcatag gtctgtatgc ttctggttaa attcaaaacg ttcactgtct ctctgaaaca
     1381 tattgtagta agccatcagg tgatgggcaa aactaatagg ctgggcatgt tgcagatggg
     1441 tatagcccgg cataatagtc tccacatgat tttccgccaa gtctaataaa acacctttga
     1501 gatgaaccag tttatctaag acatggccta gttgttcctt gagatacaag tgcatatctg
     1561 tcgcaacttg gtcatttcga gaacgagctg tatgtaactt ccctgcaagg ggaccaattt
     1621 tttctgtcaa caacacttcc atattcatat gaatatcttc gtttgcgata tcaaagtcaa
     1681 gttggcctgc ctctagctct tctaaaagct ctttcaagcc tgcttggatc ttttctgact
     1741 cctccaaact caaaatgcct gtctgaccca acatctgaac gtgagctaaa gaaccaatca
     1801 aatcaaattt agctaatttc tggtcaaaag atatactcgc accaaagcgc tctacccagt
     1861 cttcgacagt accttcaaag cgaccacccc ataattttgt attcttagcc atatcacatc
     1921 cacctctcta tccaatcgtc ctatttggcg cttttttgaa cctcagaatg aaccttggtt
     1981 ggaagtcccc acaacttaat aaagccaaca gctgcatctt ggtcaaaggt atctgcacta
     2041 gtatacgtcg ccaaattttc atcataaagt gaatttggtg atttccgagc cacgacctga
     2101 gcgcttccct tatagagttt gacttttgca gtcccattga caactttttg agtctcttta
     2161 atataagcaa tcaaggcttg cgtagccgga ctaaaccaca aagcattata aatgagattt
     2221 gataattcat tttctatgat tggtttaaaa tgagccactt ctctcacaag cgtcaaatcc
     2281 tcaatttcct tatgagctgt cagcaaggtc actgcccctg ggcattcata gatttctctt
     2341 gatttaatcc caactaagcg attttccacg tggtcaatcc gtccaacccc atgtttccca
     2401 gctatttcat tgagtttttg aatcaaatct gccaatttta acacttctcc attgagggaa
     2461 acagggacac cttcactaaa ctcaatatca atgtattctg gcatatctgg agccgcctct
     2521 ggagaagatg tgatgccaaa ggcttcttct ggagcctgat tccatggatt ttccaaaata
     2581 ccacattcat tggcacgtcc ccaaagattt tggtcgacag agtaaggatt gtcaaggtca
     2641 gcaggaacag gcaccccatt ttccttggca taatgaattt cttcctccct agaccatttc
     2701 cattcacgaa ctggcgcaat cacttttaaa ttgggatcca aggctgcaat cgatacttca
     2761 aaacgtactt ggtcattccc cttacctgta caaccatggg caattgtagt cgctcctgtc
     2821 tgatgcgcta tttcaaccaa ttttttagaa ataagagggc ggctcaaggc agataccaag
     2881 gggtactttt gttcataata ggcatgtgac tgaagagcca ctagcacata atctgtagca
     2941 aattcgtcct taacatcaat aacataagat tcgactgccc caaccttgag agccttatca
     3001 tggatgaagt ctaagtcttt cccttcaccc acatccatac aaactgcaac aacatcatag
     3061 tctttcttta accaggtaat agcaactgat gtgtctaaac cgccagaata ggccaaaatt
     3121 actttttctt tactcatgtt cttttccttc tttctatttt tcaatggacg ctttaaaggc
     3181 atcctcaatg agtttgtcta attctccaga cttcttcaac ttttgaatgg ttttatcgac
     3241 tgcctctttc aattccttgc tatctttttt catggctacc gcgtaggaat catcttgctc
     3301 tttttcaaaa ttgaggtctg cgattgctaa atcaggatta ttttccacaa atcccttggc
     3361 aacaggttct tcaaagataa cggcatccac ttgtcctgat tttaaatctg tgattaaatt
     3421 cccattttta ggcagagata cgagggaaga attttgtagc aagtctttcg ccatcgtctc
     3481 ttgaatcgaa ccattctgtg ctccgacctt tttctgcgct aagtcgttga cagactgata
     3541 agtggccaag tcagattttt tgacaatgag tttatttttt gcagtatagt agggagttga
     3601 aaaatcgaac accttgctcc gttcatctgt cttagaaaca ccagatatgg caagatcggc
     3661 ttttcctgat tgaacactag ccagtacatt gtcaaaactc atgggagata gttccaattc
     3721 gacacctagt tctgttgcga tagctttggc taattcgata tctgaaccca caatctgatt
     3781 tttcccatct accacttttt gatattcaaa tggagcaaaa tctggattga gagctacaac
     3841 caattttcct ttggacttga tggcttcaat tccagccgat tgattgttgc tacaagcaaa
     3901 tagtaggggg agcatcacta aaccaaacat cgtcatcaac accttcttca tcttattcat
     3961 aacaaagcct cctttatgtt tattcttttt ggttgtataa ataatactcc tatactcatc
     4021 ctttgtcaag tctaaattga aaaaattttc aatttgaaac aaaaaaacct agaaatacta
     4081 cataaaaatg tcatttctag gcgtatttat gctatttctc tctggaaaat atgagtattc
     4141 agtcagtcaa aaggagctga acgaactcat ttcccctcgc ccaattcaat gatccgatga
     4201 cattgttggg ctacataagc atcgtgggtc acaataatga ctgttttccc ctctcgattc
     4261 atctctaaga gaaacttcaa gaccaaatct ctattttcag ggtccagaga acctgttggt
     4321 tcatcggcta aaatcagctg gctgggtttt aagatggctc tagcaactgc aattcgttgt
     4381 tgctccccac cagataactc ggaaaccttt tggtgcaaag tagctgacaa acctaccctc
     4441 tctaaaatct cttccacctt tttgagcttg tctttcttag acaatttcac atatttcagc
     4501 gccagcataa gattgtactc gaccgtttca tcatcaatca gggcaaaatt ttgaaacaga
     4561 taagagatat gttcacggat tattgtttgc gacttagcag aattaactgc tagatttgtc
     4621 tgaccaaaaa tctcataccg tccgctataa tcaccatcta tcaaacctaa taaatttaac
     4681 aaggtcgact taccactacc actcttgcca acaatggcta ccaaatcccc ctgatcaatc
     4741 ctgagagata agttatccaa aatcactttt cctccaatgg ttttggtaat attttccaac
     4801 tcaatcataa gatgccccct ttcaataact ctactagact tcttttctcc atcctagaag
     4861 ccaagcctag cacaaatagt atatccaaac atgtaaaacc tgcaaaaagt aggagtggca
     4921 agagcgcatg ggcaaagaaa atcaagacta gaagaggcag actatagccc agcaagagca
     4981 gaacgaggag agggcggtag cgatcgacca gtttccaccc cataaacttc ttggtaataa
     5041 tatccctgcg cttcaataag aaagttatta ctagtaagaa gtaggaaatc atcatgctaa
     5101 ggagaccaaa caaagcaaag agtaggttaa aattccgaac agcatctcga taagaatcca
     5161 ctttctcttg ttgaatggct tgaatagatg aaaattttaa ataatttcca tctgacaatt
     5221 tctcaactaa ctctgtaatc tctttttgat gttgaaccgt attttcaatt ttaatcggat
     5281 tatttaagcc agttgttgac agggaggctt tctcatccca catcatatca gaatcattga
     5341 ccaagctaat aattggattg gagagatttt cctttcgctt atcactatat gggaaaaatg
     5401 accaatctcc ttcataatag gcaatctcga catccatctc ctctatcgtt cgtttttgct
     5461 gctcttcata cttcatcgaa tgaaaggcaa ttaacttccc caagagctga tttttatctt
     5521 cttcaccttt cgtacttgct ggcatcaaaa taactttttt aataccggta tctggtagct
     5581 tgaatccctt gctctttaga aaatggcgat tggcatagta aacatccacc gtatctgtta
     5641 actgatattg ctgaatctgt tctgattgga caaaattttt tacaggaaga ctgctactct
     5701 gcacatagcc cgcctgcgtt ttttctacca aatcctgata aaatcgatag aaataatcca
     5761 atgccttccc tgagcctgcc aactgctctt gccacaagtt atcattgagt tggaaagttt
     5821 ctaaggtcag ataatttcct tggctgatcc actgtttctg gtaatccaac tctttgtttt
     5881 cttgctccaa actttttcct accccaacta gcaagactgt cagtaaaatg gtagttccaa
     5941 ttttcatcaa ataattaaag agtagaccaa atttgaaaga tgaaaaacct ttcagcagag
     6001 agctgattgt cattttttgg attaagaggt aagtcaacca actgataaag agataaagct
     6061 gcaacagcaa aaaatgagac aaccacagca taggaaacaa atcttttggc ttataatcaa
     6121 gcaagaaaaa cacgcctaga ttgatcacaa gagccccacc taggaggagg taaaggttgc
     6181 cttttacaac atcagctaaa acagccctat cttgaaaacc aagtaatttt tgtaccccaa
     6241 ctcttttcat ctccatcatc ggttgataca ctgtcactaa cacaagaagc aaaatagcca
     6301 agacaaaaac aatggcagat aaaagcaaat ctcgatttat gacttccact gcacttttgt
     6361 aggtcggctc tagcaaggta gcctggtcta tcttgaaaaa atcgctccat ttctgtacaa
     6421 tcctatcctt gtccatctct tgtgtagaag ttatcgtata gcgaccattt aaactacgag
     6481 atgtatcctt gatataggtt tgaaaagtca taagctgaat aggtttggct tttagaaagg
     6541 tcggaatcgt accaagttta ttggaaattt ctttattact atagactcct tcaccatctg
     6601 tggtaaaatc aagagaagaa atcccaaact cttggtaggg gaaggtatct ttatcaaaaa
     6661 caccagactt gaccacctca tcaccactgt ctgttttgat gatggagact ttatactcct
     6721 ttgatacatc ctcaaaaaat cgaagaacag acgctgcagg ttcgttaata tctttcaaat
     6781 acaaatccaa agaatctaca gtctttccca tttcttgaat ccgaaccact tctcgtgtat
     6841 agtttacatt aaaaacaaaa aaagtggtac acagaagcag gagtaacata cagaaattac
     6901 tgattttttt cataaaagtg cctccactac agttttccct ctccaaatag gagagggaag
     6961 aattttactc aaatccatag aaatattcca ttcctgttgg cggaatcttg gtgagggaag
     7021 ctctagccca tgcctcaggt tttgcataat ccttagaggt tctaccccca tgtctaacag
     7081 ttgctgtatg atatcgagta gaatgtaagt aatcagaata tccaaaagtt cctgtccaac
     7141 ctactccata gttccattga ccaccctcaa cccatactgc taaagcattt gtagtagcaa
     7201 gtataccaca taccattaaa gatgcaacac caattttaac taattttctc atgatttatt
     7261 tctcctatta tcgagagctg ttccgtaaaa acagctatag taatttatca acaagtatcg
     7321 tagtccattg gactcacctt ccttctatct catttcattt tttaacaacg aacttactct
     7381 taaccgggag aatggtgcgc ttctgtatca ggtccttgaa caaccagagt agtattaaac
     7441 acactctaac tattactcaa gaatcagtcc aataaacaat atggatagcg ctttcaaatt
     7501 tttaaactcc catcccatcc ttttatctat ataataagtg aaaatataat aactgtcaag
     7561 taatcgaagt gaattttata aaaaaaatta caagccaaat ttgcaaagtt tacaataagc
     7621 cgctagggca atcgtctatc agaatgtccg tttatttgtc aataatccga gaaaatcttg
     7681 caacgcttag aagtctataa aaactatcaa catttatatg acttgcgaat agcaatcctg
     7741 ctcaaccttt ccacactcta tctatacaat caagataaaa acatgtgtaa gcaaatctgc
     7801 tacactttac tggaggacgc caagaataag aaaagctacg ataggctggc tatctgctat
     7861 gtccctattg ggatttgtac agacgattct aaacttatcc aaaaagggtt ctcccttctg
     7921 gagctaaccg aggaaacttc tatgctgtct catctcaaaa aagaagtaga gatctattac
     7981 caagcgaagg aaagataaaa aagccgaggg atttcctcga ctctttacat attatttgtt
     8041 cgttagttct taataccagc cattgttaag ccagaagttt ttagcagcag tccatgaacc
     8101 gtaacgtcct gcaacgtagg catctgctac acgttcttgg ttttcagctg agtagtcacc
     8161 gttcaggtat gaatctgtta attggtaacg tccgatataa cgtccatttg tagctgtata
     8221 gctaccacct gattcttttt gagcgatcca ttctttggct tctgcttcag atccgcttac
     8281 agttgaaaca actgtttcac ttactactgg agtttctgct actggagctg aaacagtttc
     8341 atcttgagcg gctggtgccg cataagtagc tggcgctggt gttgcaacag gcgctacagg
     8401 gccatcgata accaactctt gatcaacata aatcaaatga atgttatcaa tgtggttgtt
     8461 ttctgccaat ttttcaactg ttgtgttgtg agtttcagcg atttctgaaa gtgtatcacc
     8521 ttctttaaca gtgtaagttg atgattcttg agcagataca aatgatggag caaatactgc
     8581 aaacaaggca gctactcctg caagagttgc tttaatcttt ttagttattg atttcatatt
     8641 cgaaaattct ccttctttct atagttctat catacccttt aaatattacc gtttctt
//
LOCUS       DAVLVP010000075         8667 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.075, whole genome shotgun sequence.
ACCESSION   DAVLVP010000075 DAVLVP010000000
VERSION     DAVLVP010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8667
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.075"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..215)
                     /locus_tag="VYN69_001611"
     CDS             complement(<1..215)
                     /locus_tag="VYN69_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001093487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEV1533852.1"
                     /translation="MNTKKMSQFEIMDTEMLACVEGGGCNWGDFAKAGVGGGAVRGLQ
                     LGIKTRTWQGAATGAAGGAILGGVAYAA"
     gene            complement(418..708)
                     /locus_tag="VYN69_001612"
                     /pseudo
     CDS             complement(418..708)
                     /locus_tag="VYN69_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            complement(754..987)
                     /locus_tag="VYN69_001613"
     CDS             complement(754..987)
                     /locus_tag="VYN69_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEV1533853.1"
                     /translation="MTEKEIFDRIVTIIQERQGEDFVVTESLSLKDDLDADSVDLMEF
                     ILTLEDEFSIEISDEEIDQLQNVGDVVKIIQGK"
     gene            complement(993..1985)
                     /gene="plsX"
                     /locus_tag="VYN69_001614"
     CDS             complement(993..1985)
                     /gene="plsX"
                     /locus_tag="VYN69_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000717456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEV1533854.1"
                     /translation="MKKIAVDAMGGDYAPQAIVEGVNQALSDFSDIEVQLYGDEAKIK
                     QYLTATERVSIIHTDEKIDSDDEPTRAIRNKKNASMVLAAKAVKDGEADAVLSAGNTG
                     ALLAAGFFIVGRIKNIDRPGLMSTLPTVDGKGFDMLDLGANAENTAQHLHQYAVLGSF
                     YAKNVRGIAQPRVGLLNNGTESSKGDPLRKETYELLAADESLNFIGNVEARDLMNGVA
                     DVVVADGFTGNAVLKSIEGTAMGIMGLLKTAITGGGLRAKLGALLLKDSLRGLKKQLN
                     YSDVGGAVLFGVKAPVVKTHGSSDAKAVYSTIRQIRTMLETDVVAQTAREFSGE"
     gene            complement(1982..2752)
                     /gene="recO"
                     /locus_tag="VYN69_001615"
     CDS             complement(1982..2752)
                     /gene="recO"
                     /locus_tag="VYN69_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEV1533855.1"
                     /translation="MIQSITSQGLVLYNRNFREDDKLVKIFTEQVGKCMFFVKHAGQS
                     KLAPVIQPLVLARFLLRINDDGLSYIEDYHEVMTFPKINSDLFVMAYATYVAALADAS
                     LQDNQQDAPLFAFLQKTLELMETGLDYQVLTNIFEIQILTRFGISLNFNECVFCHRVG
                     QAFDFSFKYGACLCPEHYHEDKRRCHLNPNIPYLLNQFQAIDFETLETISLKPGIKQE
                     LRQFMDQLYEEYVGIHLKSKKFIDSLADWGQLLKEEKK"
     gene            complement(2749..3918)
                     /locus_tag="VYN69_001616"
     CDS             complement(2749..3918)
                     /locus_tag="VYN69_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007519430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEV1533856.1"
                     /translation="MDLTKRFNKQLDKIQVSLIRQFDQVISEIPGVLRLTLGEPDFTT
                     PDHVKEAAKRAIDQNQSYYTGMSGLLTLRQAASDFVKEKYQLDYAPENEILVTIGATE
                     ALSATLTAILEEGDKVLLPAPAYPGYEPIVNLVGAEIVEIDTTENGFVLTPEMLEKAI
                     LEQGDKLKAVILNYPANPTGITYSREQLEALAAVLRKYEIFVVCDEVYSELTYTGEAH
                     VSLGTMLRDQAIIINGLSKSHAMTGWRLGLIFAPAAFTAQLIKSHQYLVTAANTMAQH
                     AAVEALTAGKNDAEPMKKEYIQRRDYIIEKMTALGFEIIKPDGAFYIFAKIPAGYNQD
                     SFAFLKDFAQKKAVAFIPGAAFGRYGEGYVRLSYAASMETIKEAMKRLEEYMREA"
     gene            4067..5077
                     /locus_tag="VYN69_001617"
     CDS             4067..5077
                     /locus_tag="VYN69_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family putative sulfate export transporter"
                     /protein_id="HEV1533857.1"
                     /translation="MSFLSKNGAGILVCLLISILSWYLGGFFSVIGAPVFAIFMGMFL
                     HPFLSSYKQLDVGLTFSSKKLLQYAVILLGFGLNISQVFAVGQSSLPVILSTISIALI
                     VAYLFQRFFALDTKLATLIGVGSSICGGSAIAATAPVIHAKEKEVAQAISVIFFFNVL
                     AALIFPTLGTWLHLSNDGFALFAGTAVNDTSSVTATASAWDSLYQTNTLESATIVKLT
                     RTLAIIPITLFLSYWQSREQKNKQGLQLKKVFPLFILYFILASLLTTLLTSLGVSSSF
                     FTPLKQLSKFLIIMAMSAIGLKTNLVAMVKSSGKSIVLGAVCWIAIILTSLGMQTLIG
                     IF"
     gene            complement(5282..5362)
                     /locus_tag="VYN69_001618"
                     /pseudo
     CDS             complement(5282..5362)
                     /locus_tag="VYN69_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(5397..5834)
                     /locus_tag="VYN69_001619"
     CDS             complement(5397..5834)
                     /locus_tag="VYN69_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEV1533858.1"
                     /translation="MSQEFINPSDGVIRQYLATSKTLAVVGLSDREETTSNRVTKEMQ
                     ARGYKIIPVNPKAAGGEILGEKAYASLAEIPFPVDIVNVYRRSEFLPDVARDFLKADA
                     KIFWAQLGLESLKAKEILRDGGCDDIVMNRCIKREHTRLIEEA"
     gene            complement(5919..8552)
                     /gene="polA"
                     /locus_tag="VYN69_001620"
     CDS             complement(5919..8552)
                     /gene="polA"
                     /locus_tag="VYN69_001620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014932012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEV1533859.1"
                     /translation="MDKKKLLLIDGSSVAFRAFFALYQQLDRFKNAAGLHTNAIYGFQ
                     LMLSHLLERVEPSHILVAFDAGKTTFRTEMYADYKGGRAKTPDEFREQFPFIRELLDH
                     MGIRHYELAQYEADDIIGTLDKLAEQDGFDITIVSGDKDLIQLTDEHTVVEISKKGVA
                     EFEAFTPDYLMEEMGLTPAQFIDLKALMGDKSDNIPGVTKVGEKTGIKLLLEHGSLEG
                     IYENIDGMKTSKMKENLINDKEQAFLSKTLATIDTKAPIAIGLEDLVYSGPDVENLGK
                     FYDEMGFKQLKQALNVSSADVAEGLDFTIVDQISQDMLSEESIFHFELFGENYHTDNL
                     VGFAWSCGDKLYATDKLELLQDPIFKDFLEKTSLRVYDFKKVKVLLQRFGVDLQAPAF
                     DIRLAKYLLSTVEDNEIATIASLYGQTYLVDDETFYGKGVKKAIPEREKFLEHLACKL
                     AVLVETEPILLEKLSENGQLELLYDMEQPLAFVLAKMEIAGIVVKKETLLEMQAENEL
                     VIEKLTQEIYELAGEEFNVNSPKQLGVLLFEKLGLPLEYTKKTKTGYSTAVDVLERLA
                     PIAPIVKKILDYRQIAKIQSTYVIGLQDWILADGKIHTRYMQDLTQTGRLSSVDPNLQ
                     NIPARLEQGRLIRKAFVPEWEDSVLLSSDYSQIELRVLAHISKDEHLIKAFQEGADIH
                     TSTAMRVFGIERPDNVTANDRRNAKAVNFGVVYGISDFGLSNNLGISRKEAKAYIDTY
                     FERFPGIKNYMDEVVREARDKGYVETLFKRRRELPDINSRNFNIRGFAERTAINSPIQ
                     GSAADILKIAMIQLDKALVAGGYQTKMLLQVHDEIVLEVPKSELVEMKKLVKQTMEEA
                     IQLSVPLIADENEGATWYEAK"
BASE COUNT         2389 a         2209 c         1566 g         2503 t
ORIGIN      
        1 gctgcatagg ccacacctcc aagtatagct cctcccgcag caccagttgc tgcaccttgc
       61 catgttcttg ttttaattcc tagctgaaga cctcgtactg ctcctcctcc aacacctgct
      121 ttggcaaaat ctccccaatt gcatccgcca ccttcaacgc aagcaagcat ctcagtatcc
      181 ataatttcaa attgtgacat cttttttgta ttcataacga atacctcttt tttattttta
      241 atatttgtct tgttacaaac ttgacaagtt tagtataaca gtatctatta atttttttca
      301 tccaaatctt gaattggcat cgaaacgtct tgaattagct tttttgtttc aaaatcatct
      361 ctatttttaa aaaagatgtt ttctaatcac ttttttacta ttagacttcc tgcaaaacta
      421 gaatcctagt tcatgattga taataccagc aatcaaattc attcgtaatc cgaagcgttt
      481 acgatgattt cgataggttg ttgaaaacat tttaaacgtt tttactttgg caaagatatt
      541 ctcaaccttg cttctctcct tagatagcgc atgattacag gctttatctt caactgttag
      601 cggcttgagt ttgctggatt tacgtggagt ttgtgcttga ggatatatct tcatgagccc
      661 ttgataacca ctgtcagcca agattttacc agcttgtccg atatttctca tttctaaaaa
      721 ccatctactt ccgttgactt ggaactccga ttgctatttt ccttgaatga ttttaaccac
      781 atctcctacg ttttggagtt ggtcaatttc ttcatcgctg atttcgatac taaattcatc
      841 ttccagagtc aagataaact ccatcaagtc aacagaatcc gcatccaaat cgtctttcag
      901 actcaaggat tctgtcacga caaagtcctc tccctgtcgc tcttggataa tggtcacaat
      961 acggtcaaaa atttcttttt ctgtcatctc ttttattctc ctgaaaattc acgcgcagtc
     1021 tgggcaacca cgtctgtttc tagcatggta cggatctgac gaatcgtact ataaacagcc
     1081 ttggcgtcgc ttgagccatg agtcttgaca acaggtgcct taacaccaaa caagaccgct
     1141 ccaccaacat ctgaataatt gagctgtttt ttcaaacctc tgaggctgtc cttgagaagg
     1201 agggcaccta gtttcgctcg aagaccacca cctgtaatag ctgtcttgag caagcccatg
     1261 attcccatag ctgtcccttc gatggatttg agcacagcgt ttcccgtgaa accatctgcc
     1321 acaacaacat ctgcaacgcc attcatcaaa tcacgcgctt ccacgtttcc gataaagttc
     1381 aaactttcat cagccgccag taattcataa gtttccttac gaagcgggtc gcccttgcta
     1441 ctctctgttc cgttgttgag caaaccaacg cgtggttgcg caatgccacg gacattttta
     1501 gcatagaagg aacctagaac cgcatattga tggaggtgct gggctgtatt ttctgcattg
     1561 gcaccaaggt ctagcatgtc aaaacctttt ccatcaacgg taggcaaggt agacatgagt
     1621 ccaggacggt cgatattctt gatacgaccc acgatgaaga atcctgctgc caacaaggca
     1681 cctgtattcc cagccgaaag gacagcgtct gcttcaccat ctttgacagc cttggctgcc
     1741 aataccatac tggcattttt cttattccga atagctctcg taggttcatc atccgaatca
     1801 atcttctcat ccgtatggat aatgctgacg cgctctgtcg ctgtcagata ttgcttgatt
     1861 ttagcttcat ctccgtaaag ttgaacctcg atatctgaaa agtcagatag ggcttgattg
     1921 acaccctcaa caatggcctg aggtgcgtaa tcgcccccca tggcatctac tgcgattttt
     1981 ttcatttctt ttcctctttt agtaattgtc cccagtctgc tagggaatca ataaatttct
     2041 ttgattttag gtgaatccca acgtactctt catataattg atccataaat tggcgtagct
     2101 cttgcttgat tccaggcttg agcgaaatgg tctccaaagt ctcaaaatca atagcttgaa
     2161 attgattgag cagatagggg atattgggat tgagatgaca acgtctctta tcctcatgat
     2221 aatgctctgg acagaggcag gctccatatt tgaaagaaaa gtcaaaagcc tgaccaaccc
     2281 gatggcagaa gacacactca ttaaaattga ggctgattcc aaatcgagtc aaaatttgaa
     2341 tttcaaaaat attggtcaaa acctgataat ccaagcctgt ttccatcaac tccaaagtct
     2401 tttgcaaaaa agcaaacaag ggagcatcct gctgattgtc ctgcaaacta gcatctgcaa
     2461 gagctgccac ataggtcgca taggccatga caaagaggtc actattaatc ttgggaaaag
     2521 tcatgacctc atgatagtct tcgatgtaac tgagtccgtc atcattgatt cgcaagagaa
     2581 atcgtgccag caccaagggc tgaataacag gcgccagctt agactgacca gcgtgtttga
     2641 caaaaaacat gcatttgcca acctgctctg taaaaatttt gacgagcttg tcatcctcac
     2701 gaaaattgcg attgtaaagc accaagcctt gactcgtgat agactgaatc atgcttctct
     2761 catgtactcc tcaagtcgtt tcatggcttc tttgatagtc tccatactgg ctgcataaga
     2821 taggcggacg tagccttccc cgtaacgtcc aaaggctgca ccagggataa aggcaacggc
     2881 cttcttctga gcaaaatcct tcagaaaagc aaaggagtct tgattgtagc ccgctggaat
     2941 tttagcaaaa atatagaagg caccgtctgg tttgataatc tcaaaaccaa gagcagtcat
     3001 tttttcgatg atatagtccc gacgctggat atattccttc ttcatgggct ccgcatcatt
     3061 tttaccagcc gtcaaggctt ctaccgcagc atgttgcgcc atggtatttg cggcagtgac
     3121 caagtattgg tgactcttga ttaactgggc tgtgaaggcc gcaggagcga aaatcagccc
     3181 caaacgccaa cctgtcatgg catgcgattt agacaaacca ttgataataa tagcctggtc
     3241 tctcaacatc gttcctagag acacatgggc ttcgcctgtg taggtcaatt ctgagtaaac
     3301 ctcatcacag acaacaaaaa tttcgtactt gcgtaaaaca gctgccaagg cctctaactg
     3361 ctctcgactg taggtaattc ctgtcggatt ggctggatag ttgagaataa ccgccttgag
     3421 cttatcaccc tgctccaaaa tggccttctc caacatctca ggagtcaaga caaaaccatt
     3481 ttcagtcgta tcaatctcaa caatttctgc cccaactaag ttaacaatcg gttcatagcc
     3541 tggataagca ggagctggca aaagtacctt gtctccctct tccaaaatag ccgtcaaagt
     3601 cgcagataaa gcctctgtcg ccccaattgt aaccaaaatt tcattttcag gagcatagtc
     3661 cagttggtac ttttccttaa caaagtcact ggctgcctga cgtagagtca gcagaccact
     3721 catccctgta tagtaggatt ggttctgatc aatcgctcgc ttggccgcct ccttgacatg
     3781 gtctggcgtt gtaaaatcag gttcccccaa ggtcaaacgc aagaccccag gaatctccga
     3841 aataacctgg tcaaactgac gaatcaacga aacttgaatt ttatctaact gtttattaaa
     3901 gcgcttagtt aagtccatag atacctccta ctttcaaaac gtatctctat tatactacaa
     3961 aagccccccg accgcaaaaa tacattagag aatagctttc cactttctat tttacttttc
     4021 ctgtttacag gtctataatg gtaacagatt ctatttggag gtttttatgt catttctatc
     4081 aaaaaatgga gcaggcatct tggtctgcct tctcatttcc atcctatctt ggtacttagg
     4141 aggattcttc tctgtgattg gcgcacccgt ttttgcgatt tttatgggca tgttcctaca
     4201 tccctttctc tcgtcttata aacaactgga tgttggattg acctttagtt ccaagaaatt
     4261 gctccagtat gccgttatct tacttggttt tggtctcaat atctctcaag tcttcgcagt
     4321 tgggcaatct tcactccctg tcatcctttc caccatttca atagccttga ttgttgccta
     4381 cctcttccag cgcttctttg ccctggatac aaaactggct accttgattg gagtaggttc
     4441 ttctatctgt gggggttctg ccattgcagc gacagcgcct gttatccatg ctaaggaaaa
     4501 ggaagtagcc caagccatct ccgttatctt tttcttcaat gtcttggctg cgctcatctt
     4561 tccaaccctc ggcacctggc ttcatctatc caatgacggc ttcgccctct ttgcaggaac
     4621 tgcggtcaat gatacttcct ctgtaaccgc cacagctagc gcttgggaca gtctctacca
     4681 gaccaatacc ctcgaatccg caaccattgt caaactcacg cgcaccctag ctatcatccc
     4741 catcactctc tttctctcct actggcaaag tcgcgagcaa aaaaataagc aaggcttaca
     4801 actgaaaaaa gtcttcccac tttttatcct ttacttcatc cttgcatcgc tcctaaccac
     4861 ccttctgacc tctctcggtg tgtctagtag tttctttacc cctctcaaac aactctctaa
     4921 attcctcatt atcatggcca tgagtgccat cggtctcaaa actaatctgg tcgctatggt
     4981 caaatccagt ggaaaatcca ttgttctcgg agccgtctgc tggattgcca tcatcctcac
     5041 cagccttggt atgcagaccc ttatcggcat tttctaatac tcttcgaaaa tctcttcaaa
     5101 ccacgtcaac gtcgccttgc cgtaggtata tgttactgac ttcgtcagtt ctatctgcaa
     5161 cctcaaaacg gtgttttgag ctgacttcgt cagttctatc tgcaacctca aaacggtgtt
     5221 ttgagctgac ttcgtcagtt ctatctacaa cctcaaaacg gtgttttgag ctgacttcgt
     5281 cagttctatc tacaacctca aaacagtgtt ttgagcagcc cgcggctagt ttcctagttt
     5341 gctctttgat tttcattgag tataacacaa aaggtagccc atcagctacc tttttcttat
     5401 gcttcctcaa tcaagcgagt atgttctctc ttgatacagc gattcatcac gatatcatca
     5461 catccaccat cacgcaaaat ctctttcgct tttaaacttt caagtcctag ctgtgcccaa
     5521 aaaatcttgg catcagcttt gagaaaatca cgcgccacat cgggcagaaa ttcactgcga
     5581 cgataaacat tgacaatatc tacaggaaaa ggaatttcag cgaggctagc ataagccttt
     5641 tcacccaaga tttcgccacc tgccgccttg ggattgactg ggatgatttt atagccccga
     5701 gcctgcattt cctttgtcac tcgattgctg gttgtttctt cacggtcaga caaacccacc
     5761 acagcaaggg ttttactcgt tgcgagatac tgacgaatca cgccatcact tggattgata
     5821 aattcttgac tcatagaaaa cctccttttt catcagtata gcacattttg aaaaggtttg
     5881 cagaattcta ctacaaaaaa ggaggactag cccccttttt atttagcctc gtaccaggtt
     5941 gccccttcat tctcatctgc gataagagga acactgagtt gaatggcttc ttccatggtt
     6001 tgtttcacca attttttcat ctctaccaat tcagatttag gcacttcaag gacgatttca
     6061 tcgtgcactt gtaacagcat cttagtctga taaccacctg caaccaaggc tttatccagc
     6121 tgaatcatgg caatcttgag aatatctgct gccgaaccct ggataggtga gttgatagca
     6181 gttcgctccg caaaaccacg aatattgaag ttgcgcgaat tgatatctgg caactcacgg
     6241 cgacgcttaa agagggtctc tacatagccc ttatcacgcg cctcccgcac cacttcatcc
     6301 atgtagtttt taatacctgg aaaacgttca aagtaggtat caatgtaggc tttggcttcc
     6361 ttacgactaa ttcccaaatt attagacaag ccaaagtctg aaatcccata aaccactcca
     6421 aagttaactg ccttggcatt gcgacgatca tttgcagtca cattatcagg acgctcaatg
     6481 ccaaagaccc gcatggctgt cgaagtatgg atatctgccc cctcttggaa ggccttaatc
     6541 aagtgctcat ccttagaaat atgcgccaaa acgcgcaatt caatctgtga atagtcagag
     6601 ctgagtagca cactatcctc ccactctggc acaaaagcct tccgaatcaa gcgcccttgt
     6661 tccaatcggg caggaatatt ttgcaagttt ggatccacac tagacaaacg cccggtctgg
     6721 gtcaaatcct gcatatagcg agtatgaatc tttccatcag ccaaaatcca gtcctgcaag
     6781 ccaattacat aagtagattg aatcttagca atttgacggt aatccaggat tttcttaaca
     6841 atcggagcaa taggagcgag acgctccaac acatccactg ctgtcgaata acctgtcttg
     6901 gttttcttag tgtattctag aggaagtccc aatttctcaa agagaagcac gcccaactgc
     6961 ttaggcgagt tgacattaaa ctcctcacca gccagctcgt aaatctcttg agtcagtttt
     7021 tcaatgacaa gctcattttc agcctgcatc tcaagcaagg tctctttctt gaccacaatc
     7081 ccagcaattt ccatcttggc aagaacaaaa gccagaggtt gctccatatc ataaagaagc
     7141 tctaattgcc cattttcgct gagtttttca agtaaaatag gctctgtttc taccaaaaca
     7201 gcaagtttac aagctaagtg ttccaagaat ttctcacgtt caggaatggc ctttttaaca
     7261 cccttaccgt agaaagtttc atcatcaacc aagtaagtct gaccataaag actagcgatg
     7321 gtcgcaattt cattgtcctc cacagtcgaa aggaggtatt tagccaaacg gatgtcaaaa
     7381 gcaggcgcct gcaaatccac accaaaacgt tgcaaaagaa ctttaacctt cttaaagtca
     7441 taaactctca gagatgtttt ttctaagaaa tccttgaaaa tcgggtcttg caacagctca
     7501 agcttgtctg tggcatagag cttatcccca caagaccagg caaatccaac caaattatcc
     7561 gtatgataat tctcaccaaa aagctcaaag tggaagatag actcttcact cagcatatct
     7621 tgactgattt ggtcaacaat agtaaaatcc aaaccctcag ccacatcagc tgacgacaca
     7681 tttaaagcct gcttgagctg tttgaatccc atctcatcgt aaaatttccc aagattttca
     7741 acatctggac cactatagac caagtcctct aaaccaatcg caatcggtgc cttggtatca
     7801 atggtcgcta gtgttttaga caaaaaggcc tgttccttgt cattgatgag attttccttc
     7861 atcttagaag tcttcattcc atcaatattt tcataaatcc cctcaagcga accatgctcc
     7921 agcaagagct taatacccgt cttttcaccg actttggtca ccccagggat attatccgac
     7981 ttatcaccca tgagcgcctt gagatcgata aactgagctg gtgtgaggcc catttcttcc
     8041 atgaggtaat ctggcgtaaa ggcctcaaac tcagccacac ctttcttgga aatttcaacc
     8101 accgtatgct catccgtcag ctgaatcaaa tccttgtccc cactgacaat ggtaatatca
     8161 aaaccatcct gctctgctag cttatccagc gtcccaatga tgtcatccgc ctcatactga
     8221 gcgagctcat agtgacgaat ccccatatga tccagcaact cacgaatgaa aggaaattgc
     8281 tcacgaaact catcaggagt cttggcccga ccacccttat agtccgcata catctctgtc
     8341 cggaaggtcg tctttcccgc atcaaaagcc accaaaatat gactcggctc aacccgctcc
     8401 aacaaatgac tcaacatcaa ctgaaaacca taaatcgcat tggtatgcaa accagccgca
     8461 ttcttaaaac ggtccaactg ctgatacagc gcaaaaaacg cccgaaaagc tacagaagac
     8521 ccatcaatca ataataattt tttcttatcc atacacccat tataaaggaa agaatcaaaa
     8581 aataccattg ggaagagcta gagcaagtat ttttcaaact ttttccgaac aaatagatag
     8641 agccagagaa tctagtaaac ctagatt
//
LOCUS       DAVLVP010000076         8512 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.076, whole genome shotgun sequence.
ACCESSION   DAVLVP010000076 DAVLVP010000000
VERSION     DAVLVP010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8512
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.076"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            444..521
                     /locus_tag="VYN69_001621"
                     /pseudo
     CDS             444..521
                     /locus_tag="VYN69_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            1067..2428
                     /gene="trpE"
                     /locus_tag="VYN69_001622"
     CDS             1067..2428
                     /gene="trpE"
                     /locus_tag="VYN69_001622"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HEV1533860.1"
                     /translation="MERIIHGDVLSPILAYMRLKGQHKVILESIPRDKETARFSILAY
                     NPVFEIKFENGVLYQNGQVIDRDPLDFLYEVIHKSQHHSELPFGGGAIGFVGYDMISL
                     YEEIGQIPEDTIGTPDMHFFVYESYIVFDHKKEKIHVIEDALYSERSQEALEKSLNQV
                     LEELRIPAPNEFEDLDLSPLDFKPHIAPHKFEVMVETARDLIRNGDMFQCVLSQRFSA
                     EVIGNPFDFYRNLRVTNPSNYLYFYDFGDYQIIGASPESLVSVKNGIVTTNPIAGTRP
                     RGATDEEDKALATDLLSDEKETAEHRMLVDLGRNDIGRISETASVQVTKYMEVELFRY
                     VMHLTSVVKGRLLAELTAMDALKATLPAGTVSGAPKIRAMRRIYELETEKRGVYAGAI
                     GYLSATGDMDLAIAIRTMILKNQRAYVQAGAGIVYDSIAQNEYQETINKAKSMTRIGE
                     LRP"
     gene            2425..2991
                     /locus_tag="VYN69_001623"
     CDS             2425..2991
                     /locus_tag="VYN69_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HEV1533861.1"
                     /translation="MILLIDNYDSFTYNLAQYIGNFAEVQVLRNDDSKLYEEAEKADG
                     LVFSPGPGWPVDAGKMEDMICDFAGKKPILGICLGHQAIAEVFGGKLGLAPKVMHGKQ
                     SNINFEAPSVLYQGIEDGRAVMRYHSILIEEMPEDFEVTARSTDDQAIMGIQHKNLPI
                     YGFQYHPESIGTPDGLSSIRNFIEKVVK"
     gene            3002..4006
                     /gene="trpD"
                     /locus_tag="VYN69_001624"
     CDS             3002..4006
                     /gene="trpD"
                     /locus_tag="VYN69_001624"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HEV1533862.1"
                     /translation="MKEIIEKLAKFENLSGVEMTDVIERIVTGRVTEAQIASLLLALK
                     MKGETPEERTAIAQVMRGHAQHIPTEIHDAMDNCGTGGDKSFSFNISTTAAFVLAGGG
                     IHMAKHGNRSISSKSGSADVLEALGINLDLKPAELGKVFDKTGIVFLFAKNMHPAMKY
                     IMPARLELGIPTIMNLTGPLIHPMALETQLLGISRPELLESTAQVLKNMGRKRAIVVA
                     GPEGLDEAGLNGTTKIALLENGEISLSSFTPEDLGMEGYAMEDIRGGNAQENAEILLS
                     VLKNEASPFLETTVLNAGLGFYANGKIDSIKEGVALARQVIARGKALEKLRLLQEYQK
                     "
     gene            4003..4770
                     /gene="trpC"
                     /locus_tag="VYN69_001625"
     CDS             4003..4770
                     /gene="trpC"
                     /locus_tag="VYN69_001625"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011227478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HEV1533863.1"
                     /translation="MSQEFLARILEQKAREVEQMKLEQIQPLRQTYRLAEFLKNHQDR
                     LQVIAEVKKASPSLGDINLDVDIVQQAQTYEENGAVMISVLTDEVFFKGHLDYLREIS
                     SQVEIPTLNKDFIIDEKQIIRARNAGATVILLIVAALAEERLKELYDYATVLGLEVLV
                     ETHNLAELEVAHRLDAQIIGVNNRNLTTFEVDLQTSVDLAKHFKDDCLYISESAIFTG
                     QDAERLAPYFNGILVGTALMQAENVVQRIKELQIDKG"
     gene            4757..5356
                     /locus_tag="VYN69_001626"
     CDS             4757..5356
                     /locus_tag="VYN69_001626"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HEV1533864.1"
                     /translation="MTKVKICGLSTEEAVETAVSAGADYIGFVFAPSKRQVTLEEAAE
                     LAKLIPSDVKKVGVFVSPSRVELLEAIDKVGLDLVQVHGQVADDLFENLPCASIQAVQ
                     VDGNGHVPNSQADYLLFDAPVAGSGQPFDWGQLDTTGLAQPFFIAGGLNEDNVVKAIQ
                     HFTPYAVDVSSGVETDGQKDHEKIRRFIERVKHGISGTK"
     gene            5334..6557
                     /gene="trpB"
                     /locus_tag="VYN69_001627"
     CDS             5334..6557
                     /gene="trpB"
                     /locus_tag="VYN69_001627"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HEV1533865.1"
                     /translation="MAYQEPNKDGFYGKFGGRFVPETLMTAVLELEKAYRESQADPSF
                     QEELNQLLRQYVGRETPLYYAKNLTQHIGGAKIYLKREDLNHTGAHKINNALGQVWLA
                     KRMGKKKIIAETGAGQHGVATATAAALFNMECTIYMGEEDVKRQALNVFRMELLGAKV
                     EAVTDGSRVLKDAVNAALRSWVANIDDTHYILGSALGPHPFPEIVRDFQSVIGREAKQ
                     QYRDMTGQNLPDALVACVGGGSNAIGLFHPFVEDESVAMYGTEAAGLGVDTEHHAATL
                     TKGRPGVLHGSLMDVLQDAHGQILEAFSISAGLDYPGIGPEHSHYHDIKRASYVPVTD
                     EEALEGFQLLSRVEGIIPALESSHAIAFAVKLAKELGPEKSMIVCLSGRGDKDVVQVK
                     DRLEADAAKKGEAHA"
     gene            6550..7326
                     /locus_tag="VYN69_001628"
     CDS             6550..7326
                     /locus_tag="VYN69_001628"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HEV1533866.1"
                     /translation="MPKTLTEKLNAIKAAGKGIFVPYIMAGDHEKGLDGLAETIHFLE
                     DLGVSAIEVGIPFSDPVADGPVIEEAGLRSLAHGTSTQALVETLKTIETEIPLVIMTY
                     FNPLFQYGVENFVKDLADTAVKGLIIPDLPHEHANFVEPFLADTDIALIPLVSLTTGI
                     ERQKELIEGAEGFIYAVAINGVTGKSGNYRADLDKHLAQLHQVADIPVLTGFGVSSQA
                     DLERFNAVSDGVIVGSKIVKALHQGEPIQDFIKQAVAYQK"
     gene            complement(7298..7957)
                     /locus_tag="VYN69_001629"
     CDS             complement(7298..7957)
                     /locus_tag="VYN69_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000564993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEV1533867.1"
                     /translation="MIDFYFFLVGSILASFLGLVIDRFPEQSIISSASHCDSCQTRLR
                     PLDLIPILSQVFNRFCCRYCKVRYPVWYALFELGLGLLFLLYSWELLSLSQVILITAG
                     LTLGIYDFRHQEYPLLVWMTFHLILIASSGWNLVMVSFLILGILAHFIDIRMGAGDFL
                     FLASCALVFSVTELLILIQFASATGILAFLLQKKKERLPFVPFLLLAACVIIFGKLLL
                     V"
     gene            8027..8425
                     /locus_tag="VYN69_001630"
     CDS             8027..8425
                     /locus_tag="VYN69_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000155432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1533868.1"
                     /translation="MTIRFEEKVSIENAQFVCQWSNSLGKSFQEQWMGPRIPFLLTLQ
                     ALEGVFSIFDEQEFVALIQKIRLEDSNLHIGRFFINPQKQEQDLGSQALRKFVSLAFE
                     NRDIDSISLNVFEANQRAQNLYQKEGFEIV"
BASE COUNT         2563 a         1663 c         2088 g         2198 t
ORIGIN      
        1 aaaaaaatcg taaaaaagtc ttgacaaaga aaaaacaaag tattaaaata agttcaacaa
       61 atcagaaaag taactatttt tgatcttcag ggagcctgtg gtgattgtga ataggtggtt
      121 ggaagtagtg aaagtgggct gattttgaaa atgaatttga aacaatgaaa attcggtgcg
      181 cacaccttac agtgcagctt gttgttaaac aaggcagaga tgtaaaggga tagtcccttt
      241 ataattgagg tggcaccgcg ttaccaacgc cctcacacgg aggtatattc tgtgtgtggg
      301 cttttttcta tccgtcgttt ggtttatctt ttattagggc cttaagtcgc tttgatgaac
      361 ttgagttcta tctacagctc cttgcctact actaaaagca aacaaaaagg ctggactaat
      421 acgaaaaaag ctagtaatac tcaatgaaaa tcaaagagca aactaggaaa ctagccgcag
      481 gctgctcaaa acactgtttt gaggttgcag atagaactga cgaagtcagc tcaaaatact
      541 gttttgaggt tgtggataga actgacgaag tcagctcaaa acactgtttt gaggttgcag
      601 atagaactga cgaagtcagt aaccatacct acggcaaggc gacgttgacg cggtttgaag
      661 agattttcga agagtataaa ggtgtaccga gatgaacatc tcttcatcaa tgtctctggt
      721 cgtggaggca aggacctagc tgcgattgca gactactaga agctaaaaaa taatagatgg
      781 aaaaattaca gttctttctc tcacagagag cttgtggttg ctgaaaacaa gcagagaaag
      841 ctgtaaagtt gggttcatct gaaacgagag aagaaaacat cattctcaag ggagtgccct
      901 ttatcgcaca gcctgttaca ggagatatct gagacaggaa tgagagatag gagaaatcct
      961 ataattgagg tggcaccgcg aatttcgtcc tcacgcaagt tattttgcgt ggggattttt
     1021 catacagtcg ctacgaacta gaattagaac tgaaagggaa attacaatgg aacgaatcat
     1081 tcatggagat gtcttatcac caatcttggc ttatatgcgc ttaaaggggc aacacaaggt
     1141 tatcttagag agtattccga gagacaagga aacagctcgt ttttctatcc tagcctataa
     1201 tcctgttttt gagattaagt ttgaaaatgg agttctttat caaaatggtc aagtgattga
     1261 tcgggatccc ttggatttcc tttatgaagt gattcataag agtcagcacc attcagagct
     1321 accttttggt ggtggagcca ttggttttgt gggttacgat atgatttcgc tttatgaaga
     1381 aattggtcaa atccctgagg atacaattgg gacgccagac atgcatttct ttgtctatga
     1441 gagctatata gtctttgacc acaagaagga gaagatacat gtcatagagg atgcacttta
     1501 tagtgagcgc agtcaagagg ccttggaaaa atccttgaac caagtgcttg aggaattacg
     1561 cattcctgct ccaaatgaat ttgaagactt ggatttatca ccacttgact tcaaaccgca
     1621 tatcgctcca cataagtttg aggtaatggt agaaactgct cgcgacttga ttcgtaatgg
     1681 tgatatgttc caatgtgtac tcagccagcg tttctcagca gaagttattg gaaatccatt
     1741 tgacttctac agaaatctcc gcgtgactaa tccttctaat tacctttatt tctatgattt
     1801 tggggattat caaatcatcg gagctagtcc agaaagtttg gtttctgtca aaaatggcat
     1861 cgtgacaacc aatccgattg cagggacgcg accaagaggg gctacggatg aagaagacaa
     1921 ggccttggcg actgacctcc tctctgatga gaaggaaaca gcagaacatc gaatgttggt
     1981 agacttgggg cgtaacgata ttggccgcat ctctgaaacg gccagtgtcc aagttactaa
     2041 gtatatggag gtggagctct tccgctacgt catgcatttg accagtgtgg tcaagggacg
     2101 tttgcttgca gaactcactg ccatggatgc cttgaaagca acgcttcctg ctggaaccgt
     2161 ttctggagca ccaaaaattc gagctatgag acgcatctat gaactggaaa cggaaaaacg
     2221 gggcgtatat gcaggagcaa tcggctactt gtctgcgacg ggtgatatgg atttggccat
     2281 tgccatccga accatgattc tcaaaaatca aagagcctat gtgcaggctg gggcagggat
     2341 tgtctacgac tctatcgccc aaaacgaata ccaagaaacc attaacaaag caaaatctat
     2401 gactagaatt ggagaactaa gaccatgatt ttattgattg acaactatga ttcttttacc
     2461 tataacttgg cccaatacat tgggaatttt gcagaagttc aggttctgag aaatgatgat
     2521 tccaagctgt atgaagaagc tgaaaaagca gatggtctgg tcttttctcc tggtcctggt
     2581 tggccagttg atgctggaaa gatggaagac atgatttgtg attttgcagg caagaagccg
     2641 attctaggga tttgtttggg tcaccaagcc attgcagaag tctttggtgg taagctaggt
     2701 ttggctccaa aagtcatgca tgggaaacag agcaatatca actttgaagc gccatctgtt
     2761 ttgtatcaag gtattgagga tggacgtgcg gtcatgcgtt atcacagtat tttgattgag
     2821 gaaatgccag aagactttga agtgacagct cgttcgactg atgaccaagc catcatgggg
     2881 attcaacata aaaacctacc gatttatggc ttccagtacc atccagagag cattggaacg
     2941 ccagacggct tgtcttctat tcggaatttt atcgagaagg ttgtaaagtg aggaaactag
     3001 gatgaaagag attattgaaa aactagcaaa atttgaaaat ttatcaggtg tggaaatgac
     3061 ggatgtcatt gagcgtatcg taactgggcg tgtaactgaa gcgcagattg cttctctcct
     3121 cttagctctc aagatgaagg gggaaacacc tgaagaacgc acagccattg cccaagtcat
     3181 gagaggacat gcccagcata ttccaactga gattcatgat gccatggata actgtggtac
     3241 aggtggggac aagtctttca gttttaatat ttccacaact gcagcctttg tcttggctgg
     3301 tggcggtatt cacatggcca agcatggtaa ccgctcgatt tcttctaaat ctggttccgc
     3361 agatgtcctc gaagccttgg gaatcaatct tgatctcaaa ccagctgaac taggtaaggt
     3421 ctttgataaa actggaatcg tctttctctt cgctaaaaat atgcacccag ctatgaaata
     3481 catcatgcca gctcgtttgg aattgggaat tccaacgatt atgaacttga ctggtccact
     3541 gattcatcca atggctttag aaacacagct tcttggaatt agtcgtccag aactcctaga
     3601 aagtacagct caggttttga aaaatatggg tcgcaaacgt gccatcgtgg ttgctggacc
     3661 agaagggttg gatgaagctg gcttgaacgg aacaaccaag attgcacttc ttgaaaatgg
     3721 cgaaatcagc ttgtcaagct ttactccaga ggatttggga atggaaggct atgctatgga
     3781 agatattcgt ggtgggaatg ctcaggaaaa tgcagaaatt ttgcttagcg ttctgaaaaa
     3841 cgaagcaagt ccattcttgg aaacgacagt cttgaatgct ggtcttggtt tctatgctaa
     3901 tggtaagatt gatagcatca aggaaggagt tgccttggcc cgtcaagtga ttgctagagg
     3961 caaggccctt gaaaaactca gactgttaca ggagtaccaa aaatgagtca ggaattttta
     4021 gcacgaatct tagagcagaa ggcgcgtgag gtggagcaga tgaagctgga gcaaatccag
     4081 cctctgcgcc agacctatcg cttggcagaa tttttgaaga atcatcagga ccgcttgcag
     4141 gtaatcgctg aggtcaagaa agctagccct agtttgggag atatcaatct cgatgtggat
     4201 attgtgcaac aggcccagac ttatgaagaa aacggagcag tgatgatttc ggtgttgaca
     4261 gatgaagttt tctttaaagg acatttggat tatctacggg agatttccag tcaggtagag
     4321 attccgacgc tcaacaagga ctttatcatc gatgaaaagc aaatcatccg cgctcgcaat
     4381 gcaggtgcga cagtcatctt gctcattgtg gcagccttgg cagaagaacg tcttaaggaa
     4441 ctttacgact acgcgacagt gcttggtctg gaagtcttgg tggaaactca caatctagct
     4501 gaactagaag tggctcacag acttgatgcc cagattatcg gggtcaataa ccgcaacttg
     4561 accacctttg aggtcgactt gcagaccagt gtagacttgg ctaaacattt caaagatgat
     4621 tgcttgtaca tttctgaatc tgctattttc acagggcagg atgcggaacg actagcccca
     4681 tactttaacg gaattttggt agggacagct cttatgcagg cagaaaatgt ggtccagaga
     4741 atcaaggagt tgcagattga caaaggttaa aatttgtgga ctatcgaccg aagaagcggt
     4801 ggaaacagcc gtttcagcag gagccgacta catcggtttt gtctttgcac ctagtaaaag
     4861 acaggtgact ttagaagagg cagctgagtt ggcaaagctt attccttcag atgtgaaaaa
     4921 ggttggagta tttgtttcac caagtcgggt agaactgctg gaagcgattg acaaagttgg
     4981 cttggacttg gttcaagttc acggtcaggt ggcagatgat ttatttgaga atttgccttg
     5041 tgccagtatt caggctgtgc aggtagatgg aaatgggcat gtccccaatt ctcaggcaga
     5101 ttatctactc tttgatgccc ctgtggcagg aagtggccag ccctttgatt ggggtcaact
     5161 ggatacgact ggactagcac agcccttctt tatcgcaggt ggccttaatg aagataatgt
     5221 agtaaaagca attcaacatt ttactcccta tgcagtagat gtatcgagcg gagtggagac
     5281 agatggacaa aaagatcatg aaaagattag aagatttata gagagggtaa agcatggcat
     5341 atcaggaacc aaataaagat ggattttacg gaaaattcgg cggacgtttt gtcccagaaa
     5401 cattgatgac agcagttttg gagttggaga aggcctatcg tgaaagtcag gcagacccaa
     5461 gtttccaaga ggaattaaac caactcttgc gccagtatgt ggggcgggaa actcctcttt
     5521 actacgcaaa aaacttgacc cagcatattg gcggagccaa gatttatctc aaacgggaag
     5581 accttaacca tacaggagcc cacaagatta acaatgcctt aggacaagtt tggcttgcca
     5641 aacgcatggg taaaaagaaa attatcgcag aaaccggtgc tggtcagcac ggtgtggcaa
     5701 ctgcaactgc tgcggccctc tttaacatgg aatgtaccat ctacatgggt gaggaagatg
     5761 tcaaacgcca agccctcaat gtcttccgca tggagctttt gggagccaag gtcgaggctg
     5821 taacagatgg ttcgcgcgtg ctcaaggatg cggtcaatgc agcccttcgt tcatgggtgg
     5881 ctaatatcga cgatacccac tatatccttg gttctgcctt ggggcctcat ccatttccag
     5941 aaatcgttcg tgacttccaa agtgtcatcg gtcgcgaagc taaacaacag tatcgcgata
     6001 tgacaggtca aaatctgccc gatgctctag tagcctgtgt tggtggtgga tctaatgcta
     6061 ttgggctctt ccatcccttt gtagaagatg agtcagtagc tatgtatgga actgaagcag
     6121 cagggcttgg tgtggataca gagcaccacg ctgctacctt gaccaagggt cgtccgggtg
     6181 ttcttcacgg ttctctcatg gatgtgctcc aagatgccca tggtcaaatt cttgaagcct
     6241 tctctatctc agcaggtttg gactatcctg gtattggtcc agaacattct cactaccacg
     6301 atatcaaacg tgccagctat gtccctgtga cagacgaaga agccttggaa ggattccaac
     6361 tcttgtctcg tgtggaaggg attatcccag ccttggaatc tagccatgct atcgcttttg
     6421 cggtgaaatt ggccaaagaa cttggaccag aaaagtctat gattgtctgc ctatcaggtc
     6481 gtggggacaa ggatgtggtt caagtcaaag accgcttgga agcagatgca gcaaagaagg
     6541 gagaagctca tgcctaagac actaacagaa aaattgaatg ctattaaagc agctggaaaa
     6601 ggaatttttg ttccctatat catggctggt gaccacgaga aaggtttgga cggtcttgct
     6661 gaaacaattc actttttaga agatttgggt gtctctgcaa ttgaagtggg tattcccttt
     6721 tcagaccctg ttgcagatgg acctgttatc gaagaagctg gcttgcgcag tttagcccac
     6781 gggacctcta cccaggcttt agttgaaacc ttgaaaacca ttgaaacaga gattccactg
     6841 gtcatcatga cctacttcaa ccccctcttt cagtacggtg tggagaactt tgtcaaagat
     6901 ctggctgata cagctgtgaa gggcttgatt atcccagact tgcctcatga acatgccaac
     6961 tttgtagaac catttttggc agatacagat atcgccttga ttcctttagt aagcttgacc
     7021 acaggaattg agcgccagaa agagttgatt gaaggggcgg agggattcat ctatgccgtt
     7081 gccatcaatg gagtgacagg gaaatcaggc aattaccgtg cagatttgga caagcacttg
     7141 gcgcaacttc atcaagtggc cgacatccca gtcttgacag gttttggtgt atctagtcaa
     7201 gccgatctgg aacgcttcaa tgcggtgtca gatggtgtta tcgtcggttc gaaaatagta
     7261 aaagctctcc accaaggaga gccgattcag gactttatca aacaagcagt agcttaccaa
     7321 aaataatcac acaagcagca agtaagagga aaggcacgaa aggaagtctt tcctttttct
     7381 tttgcaggag aaaggccagg atacccgtcg cagaagcgaa ctgaatcaag atcagtaact
     7441 ccgttacgct aaagacgaga gcgcaagaag ctaaaaaaag gaaatcccct gcacccatgc
     7501 ggatatcgat aaaatgagcc aaaattccaa gtatgaggaa ggagaccatg accagattcc
     7561 agccagagga agctattagg attaggtgga aagtcatcca gaccagtaag ggatattcct
     7621 gatggcgaaa gtcgtagatg cccaaggtca aaccagcagt gattaggatg acttgactca
     7681 aggaaagtaa ttcccaagag taaagcagaa agaggagtcc taagcctagt tcaaagaggg
     7741 cataccagac aggatagcgg accttgcagt agcgacagca aaagcgattg aagacctgtg
     7801 agagaatcgg aatcaaatct aagggacgca agcgagtctg acaggaatcg cagtgactgg
     7861 ctgaactgat aatggattgc tctggaaaac ggtcaatgac caaaccaaga aaggaagcga
     7921 gaatgctccc gacgagaaaa aaataaaaat caatcatact tatctattcg taaaaaatgg
     7981 gagaaatagt ataatggaga aacatagata agatggtgag gaaaagatga caattcgttt
     8041 tgaagaaaag gtgagtatag aaaacgctca atttgtatgc cagtggtcca actccctagg
     8101 caaatccttt caagaacaat ggatgggacc aaggattcct tttctactga cccttcaagc
     8161 cttggaagga gtcttttcaa tctttgatga acaagagttt gtggcactta tccagaaaat
     8221 caggctagaa gacagcaatc ttcatatcgg gagatttttt atcaaccccc agaaacagga
     8281 gcaagactta ggtagccagg ctttaaggaa atttgttagt ttagcatttg aaaatagaga
     8341 tatagacagt atttctctaa atgtcttcga ggcaaatcaa agagctcaga atctttacca
     8401 aaaagaagga tttgaaatcg tttaaatggt tgaagcacct gtacgaaaat atagtagatt
     8461 gaaactagaa tagtacacct ctacttctaa aacattgtta gaaatcgatt tg
//
LOCUS       DAVLVP010000077         8497 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.077, whole genome shotgun sequence.
ACCESSION   DAVLVP010000077 DAVLVP010000000
VERSION     DAVLVP010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8497)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8497)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8497
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.077"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..189
                     /locus_tag="VYN69_001631"
     CDS             <1..189
                     /locus_tag="VYN69_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000275922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1533869.1"
                     /translation="KNLREDKDLSQCQIAQYLHCSQSAYSRIESGKQDIPTSFLKQLA
                     KLYQVTTDYILEMDKFSC"
     gene            320..472
                     /locus_tag="VYN69_001632"
     CDS             320..472
                     /locus_tag="VYN69_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533870.1"
                     /translation="MTTNKDNDTSERSGCQRKRTAGEMPMNDLTSHTHGGNYTCYKEE
                     KVWNNQ"
     gene            639..2432
                     /locus_tag="VYN69_001633"
     CDS             639..2432
                     /locus_tag="VYN69_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001864824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1533871.1"
                     /translation="MKKNNVEIIKADSLVRRRGDNVERHLKRVAAYCRVSSDSEDQKN
                     SYDSQVRHYKEYISQRSDWELADIYADEGISGTQVGKRQDFQRLINDCANGEIDYIVT
                     KAIARFARNTLDTLKYVRMLKDMQIGVYFEEENIDTLTMDGELLLTILSSVAQQEVEN
                     TSAHVKKGLKMKMQRGELVGFQGCLGYDYDVETKQISINKKEAKIVRYIFERYLEGIG
                     GKVIARELDELGYKSPRGLEHWNDTTVLGIIKNEKYKGDILMGKTFTVDPISKRRLSN
                     FGEEDKYYIKDNHEPIISKEDFEKAQEIRLRRAGNKKTAANVNGKRERYSKMYAFSSM
                     LECGFCGSILSRRSWHCRSDYRKVVWHCVTSIKKGKKFCKHSKGLEEIAIEGAFLEAY
                     RQVYHSNENLMTDLLETIESELNDNSLNKELKRITNKLRILLKKEENLVNLRLEGKVS
                     DSIYNEKYNEISSEKEFLAEEKVNIETTLKSEIDVKKRLTEFKHLLSSQKMLTEFDRA
                     VFESIVEKIIVGGVNSNGEIDPAMLTIIFKTGEIQNKDGKQFKSKRKNAKLETDKLCP
                     QNSDEDKKLYSQGTDNTRGVCSVAGSILASQ"
     gene            2537..3124
                     /locus_tag="VYN69_001634"
     CDS             2537..3124
                     /locus_tag="VYN69_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000509391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Type 1 glutamine amidotransferase-like
                     domain-containing protein"
                     /protein_id="HEV1533872.1"
                     /translation="MGGNPPMKKYSIVDKIVLSTKIKRIIIFTVFRENWEPYMKKYTE
                     VFQSQFPNLNIDYLLLDTEQIDLDSYLDADIIIIGGGNTEKYIATYVNQEFKSYIDHM
                     LNKEAKIIGFSAGALLLGEKVYVSPNDNSDHQIKIKNGLGLFSQFLISVHYDSWNDKA
                     NKDRAEELVNVPIIPLNDHSCLVLDKLGNIIEKID"
     gene            3364..3642
                     /locus_tag="VYN69_001635"
     CDS             3364..3642
                     /locus_tag="VYN69_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase"
                     /protein_id="HEV1533873.1"
                     /translation="MEKVASLGVPMCKPISIELCDDEVHSLHEWIDGRDAIDSILTYS
                     ENQQYTYGVEAGKILRKIHTIPATEVCEDWEIFFNLKIDDKISNEMIW"
     gene            3818..4057
                     /locus_tag="VYN69_001636"
                     /pseudo
     CDS             3818..4057
                     /locus_tag="VYN69_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558195.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
     gene            4201..5430
                     /locus_tag="VYN69_001637"
     CDS             4201..5430
                     /locus_tag="VYN69_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEV1533874.1"
                     /translation="MKISKRHLLNYSILIPYLLLSILGLIVVYSTTSAILIEEGKSAL
                     QLVRNQGIFWIVSLILIALIYKLRLDFLRNERLIILVILIEMLLLFLARFIGISVNGA
                     YGWISVAGVTIQPAEYLKIIIIWYLAHRFSKQQEEIATYDFQVLTQNQWLPRAFNDWR
                     FVLLVLIGSLGIFPDLGNATILVLVSLIMYTVSGIAYRWFSTILALVSAASVFVLTTI
                     SLIGVETFSKIPVFGYVAKRFSAFFNPFADRADAGHQLANSYFAMVNGGWFGLGLGNS
                     IEKRGYLPEAHTDFVFSIVIEEFGFVGASLILALLFFMILRIILVGIRAENPFNAMVA
                     LGVGGMMLVQVFVNIGGISGLIPSTGVTFPFLSQGGNSLLVLSVAVAFVLNIDASEKR
                     AKLYRELENQPMNLLLK"
     gene            5450..8146
                     /gene="ppc"
                     /locus_tag="VYN69_001638"
     CDS             5450..8146
                     /gene="ppc"
                     /locus_tag="VYN69_001638"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HEV1533875.1"
                     /translation="MSLQKLENSSNKSVVQEEVLILTELLEDITKNMLAPETFEKIIQ
                     LKELSTQEDYQGLNRLVTSLSNDEMVYISRYFSILPLLINISEDVDLAYEINHQNNID
                     QDYLGKLSTTIKLVAEKENAVEILEHLNVVPVLTAHPTQVQRKSMLDLTNHIHSLLRK
                     YRDVKLGLINKDKWYNDLRRYIEIIMQTDMIREKKLKVTNEITNAMEYYNSSFLKAVP
                     HLTTEYKRLAQAHGLNLKQAKPITMGMWIGGDRDGNPFVTAKTLKQSALTQCEVIMNY
                     YDKKIYQLYREFSLSTSIVNVSKQVREMARQSKDNSIYREKELYRRALFDIQSKIQAT
                     KTYLIEDEEVGTRYETANDFYKDLIAIRDSLLENKGESLISGDFVELLQAVEIFGFYL
                     ASIDMRQDSSVYEACVAELLKSAGIHSRYSELSEEEKCDLLLKELEEDPRILSATHAE
                     KSELLAKELAIFKTARVLKDKLGDDVIRQTIISHATSLSDMLELAILLKEVGLVDTER
                     ARVQIVPLFETIEDLDHSEETMRKYLSLSLAKKWIDSRNNYQEIMLGYSDSNKDGGYL
                     SSCWTLYKAQQQLTAIGDEFGVKVTFFHGRGGTVGRGGGPTYEAITSQPLKSIKDRIR
                     LTEQGEVIGNKYGNKDAAYYNLEMLVSAAINRMITQKKSDTNTPNRYEAIMDQVVDRS
                     YDIYRDLVFGNEHFYDYFFESSPIKAISSFNIGSRPAARKTITEIGGLRAIPWVFSWS
                     QSRVMFPGWYGVGSSFKEFINKNPENIAILRDMYQNWPFFQSLLSNVDMVLSKSNMNI
                     AFEYAKLCEDEQVKAIYETILNEWQVTKNVILAIEGHDELLADNPYLKASLDYRMPYF
                     NILNYIQLELIKRQRRGELSSDQERLIHITINGIATGLRNSG"
     misc_binding    8229..8446
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
BASE COUNT         2892 a         1299 c         1747 g         2559 t
ORIGIN      
        1 aagaatttaa gagaagacaa agatttgtcc cagtgtcaaa ttgcacagta tcttcattgt
       61 tcacagtctg cttattctag aatagaaagt ggcaagcaag atattcctac atcatttttg
      121 aaacaattag ctaaattata tcaggtaact acagattata ttttagagat ggataagttt
      181 agttgttaat aaatcctaag ccctttgaaa actgaataaa ccgaggtggg actttcttat
      241 ttgtcgagca ctttagcttg atacgccgtg ataggagcga taattattag ctataaaata
      301 gagtggttct ctatctgcca tgactacaaa taaggataat gatacttctg aacgttcagg
      361 ctgccagcga aaaaggacag cgggtgaaat gcccatgaat gatttaacca gtcataccca
      421 cggaggaaat tatacatgtt acaaggagga aaaagtttgg aacaatcagt aataaagtat
      481 actatgcaac tatcaatgct taacttttta tactcaagaa atatgttatc taaaaatgag
      541 tatgacaaga ttagaaacaa actgcgtatt aagtacacaa aatagctgac tttttgattt
      601 gaatgcggta atatagaatc aaaaggagga atttcagtat gaaaaaaaat aatgttgaaa
      661 taataaaagc tgattcttta gttcgtcgaa gaggcgacaa tgtagaaaga cacttaaaga
      721 gggttgctgc ttattgcaga gttagttccg atagtgagga tcagaaaaac agttatgatt
      781 ctcaagtaag acactacaaa gaatatattt ctcagcgttc agattgggag ctggcagata
      841 tttatgcaga tgaaggtatt tcaggaacac aagtaggaaa gagacaagat tttcaacgat
      901 taatcaatga ttgtgcaaat ggagagatag attatattgt cacaaaagca attgctagat
      961 ttgcaaggaa taccctagat actttgaagt atgtcagaat gttaaaggat atgcaaattg
     1021 gagtatattt tgaggaagaa aacatagata ccctaacaat ggatggagaa ttgcttctaa
     1081 ctattctaag ctccgttgca cagcaagaag tagagaatac ttctgcccat gtgaaaaaag
     1141 gattaaaaat gaagatgcaa cgtggtgaat tggttggttt tcaagggtgc ttagggtatg
     1201 attatgatgt agaaaccaag cagatttcaa ttaataaaaa agaagccaaa attgttcgtt
     1261 atatttttga aaggtatcta gaaggtattg gaggaaaagt aattgcaagg gaacttgatg
     1321 aacttggtta caaatcgcca agaggtttag aacactggaa tgatacaaca gttctaggaa
     1381 taataaagaa tgaaaaatat aaaggtgata ttctaatggg aaaaactttt acagttgacc
     1441 caataagcaa gagacggttg agtaattttg gagaagaaga taagtactat atcaaggata
     1501 atcatgaacc aataatatct aaagaagatt ttgaaaaagc tcaggagatt agattgcgtc
     1561 gagcaggaaa taagaagaca gctgcgaatg taaatgggaa gcgtgaacgc tattcaaaaa
     1621 tgtatgcttt tagtagcatg ttggaatgtg gtttttgtgg ttcaatatta tctagaagaa
     1681 gttggcactg ccgttcagat tatcgcaaag ttgtgtggca ctgtgttaca tcgataaaga
     1741 aaggaaagaa attttgtaag catagtaaag gattagaaga gatcgccatt gagggggctt
     1801 tcctggaagc ttacagacaa gtctatcatt caaatgaaaa tttaatgaca gacttacttg
     1861 aaacgataga atctgaattg aatgacaata gccttaacaa ggaactaaaa aggattacaa
     1921 acaaacttcg aatattacta aaaaaagagg agaatcttgt aaatctaagg ctcgaaggaa
     1981 aagttagcga ttccatatac aatgaaaagt acaatgaaat ttcctcagaa aaagagtttc
     2041 tagcagaaga gaaggtaaat attgaaacaa cattaaaatc agaaatcgat gtaaagaaaa
     2101 gactaactga gtttaagcac ttgctatctt cgcaaaaaat gcttacggag tttgatcgtg
     2161 cagtttttga aagtattgtt gagaaaatta ttgtaggtgg agttaatagc aacggagaaa
     2221 ttgatcctgc aatgttaacc ataatattca aaacaggtga aattcaaaac aaggatggta
     2281 aacaatttaa aagtaaacgt aaaaatgcta aactagaaac agataaattg tgtcctcaaa
     2341 acagtgacga ggataaaaaa ttgtattctc aaggaacaga caacacacgt ggagtgtgta
     2401 gtgttgctgg ttcgatctta gccagtcaat aaaatgatat gttttaaaaa aattataatt
     2461 tcgaaacgtg aataaacttg tttaaacttt atgtatcttt agaaagtgtg ataacttgaa
     2521 agaacaaatt tttttgatgg gtgggaatcc cccaatgaag aaatatagta ttgttgataa
     2581 aattgtatta tcaaccaaga ttaaaagaat tatcattttt acagtttttc gagaaaattg
     2641 ggaaccgtac atgaaaaagt acacggaagt ttttcaaagt caatttccta atctaaacat
     2701 tgattacttg ctcctggaca ctgagcagat tgatcttgat agctatctag atgctgacat
     2761 aattatcatc ggtggtggaa atacggaaaa atatattgct acttatgtta atcaggagtt
     2821 caaaagttat atcgatcata tgcttaataa agaggcaaaa attataggat tttctgcagg
     2881 agccctacta ttaggagaaa aagtctatgt ctcacctaat gataattcag atcatcagat
     2941 aaagataaaa aatggattag gactctttag tcagttttta attagtgtcc attatgattc
     3001 ctggaatgat aaagcaaata aggatagagc tgaagaactc gttaatgttc ccataattcc
     3061 actaaatgat cattcttgtc ttgtattgga taaacttgga aacatcatag agaaaattga
     3121 ctaactttca taatgtaaaa atggatctag gaaataaaag tgtagacgaa ctggataggt
     3181 tactagtgaa aactgcaaaa agtgttgtaa agtgaatcgg agtgttttag tagtaagata
     3241 gccataaata aaggctggtc agatgataaa aaatattgtg tgacagttca aaatcagtaa
     3301 acatatttct tgcgtgtttc tgataagtcg aagttagatt ataaaaaaat tgaatttgat
     3361 acgatggaga aagtagcttc tcttggagtt ccgatgtgta aaccaattag cattgaactc
     3421 tgcgatgacg aagtacattc tttacacgaa tggatagacg gaagagatgc aatagatagc
     3481 attttaactt attcagaaaa ccaacaatac acatatggtg tagaagcagg aaagatactt
     3541 agaaagattc atacaattcc tgctacagaa gtttgtgaag attgggaaat cttttttaat
     3601 ctaaaaattg atgataaaat ctccaacgaa atgatatggt aagtccgaaa cgctcagtgt
     3661 gtaagcttgt tgctaaaagt gtaaaaacct tggaacgaaa ggaataatag acttcctgcg
     3721 aaacaaaaat acaatacaat aaaactatgg atgatgaagc aagtaaacaa ttaagcgata
     3781 gccgtttcaa gagccttgta ggtgttcagc gcacgagatg ttagctgtgt taaaaacagc
     3841 ttatcaacgc aacgtaaacg cgcaaaaggt ggacgaaaaa ccaaattaag cctagacgat
     3901 ctcctcatgg taactattca atacatgcga gaatagcgca cttatgaaca aattgcggct
     3961 gattttggca ttcacgaaag caacataatt agtcggaggt caatggattg aagcaactct
     4021 tattcaaaat ggttttacga tttcaaattc tgccttaatt ctgtaaaaac agtaaaattc
     4081 gaaggattgt aaggtaagag tttttttctt tctgaaaaaa tggtataata gcaatcaaaa
     4141 ctagaaaata aaacggaatt tggaacagat ttgtctgtat cctagtagag tggtgatact
     4201 atgaagatta gtaagaggca cttattaaat tattccatct tgattcccta cttgctttta
     4261 tctattttgg gcttgattgt ggtctattcg accaccagtg ctattttaat tgaagaaggc
     4321 aagagcgcct tgcagttggt tcgaaaccaa ggaatctttt ggattgttag tttgatactg
     4381 attgccttaa tttataaatt gagactagat tttttgagaa atgagcgact aatcatttta
     4441 gttatattaa tagaaatgct tttattgttc ttggctcgtt ttattggtat ttcagtaaac
     4501 ggggcatacg gttggatttc ggttgcagga gtaactattc agccagctga gtacttaaaa
     4561 atcattatta tttggtattt agctcaccga ttctccaaac agcaagaaga aatagctact
     4621 tatgattttc aagttttgac tcaaaatcaa tggcttcccc gtgcttttaa tgattggcga
     4681 ttcgttctcc tagttctgat tggaagtttg ggaattttcc ctgatttagg aaatgcgact
     4741 attttagtct tggtttcctt gattatgtat acagttagtg gaatcgctta tcgctggttt
     4801 tcaaccattc tggcgctcgt atctgccgct tctgtctttg tcttgaccac tatcagccta
     4861 atcggtgttg agaccttttc aaaaattcca gtatttggct atgtagccaa acgctttagt
     4921 gcctttttta atccttttgc cgatcgtgct gatgcaggtc accagttagc taattcttat
     4981 tttgccatgg tcaatggtgg ttggtttggt ctaggtcttg gaaactcgat tgaaaaacga
     5041 ggttatttgc cagaagctca tacagacttt gtcttttcta tcgtgattga agaatttggc
     5101 tttgttggtg ccagtcttat tttagctctc ttgtttttca tgattttgcg gattatcttg
     5161 gtcggtattc gagcggagaa tcctttcaat gccatggttg cactcggtgt cggagggatg
     5221 atgttggttc aggtatttgt caatatcgga gggatttcgg gcttgattcc atctacagga
     5281 gtaaccttcc ccttcttatc ccagggtgga aatagtcttc tagtcttatc agtggcagta
     5341 gcctttgtct taaatattga tgccagtgaa aaacgcgcta agttgtaccg agaattggaa
     5401 aatcaaccaa tgaaccttct gttgaagtag gataaagaaa ggatagttta tgtctcttca
     5461 aaaattagaa aattctagta ataaaagtgt tgtgcaagaa gaagtcttga ttctaacaga
     5521 attactggaa gatattacta aaaatatgct tgccccagag acctttgaaa aaataataca
     5581 gttgaaagaa ttatcaacgc aggaagatta tcaaggtcta aaccgtctag tgactagctt
     5641 atcaaatgat gaaatggtct atatttcacg ctatttctct atcttgcctc ttttgattaa
     5701 tatttcagag gatgtggatt tagcttatga aatcaatcat caaaataata ttgatcagga
     5761 ctatttaggt aaattatcta caacgattaa attggtagca gaaaaggaaa atgccgttga
     5821 gatcctagaa cacttgaatg ttgttccggt tttgacagcc catccaacac aagtgcaacg
     5881 caaaagtatg ttggatttaa caaatcatat tcatagtctt ttgcgtaaat accgtgatgt
     5941 taagttgggg ttgatcaata aagataaatg gtacaatgat ttgcgtcgtt acatcgaaat
     6001 tatcatgcag acagacatga ttcgtgagaa aaaattaaaa gtgactaacg aaatcacgaa
     6061 tgctatggaa tattataaca gctccttttt gaaagctgta cctcatttga cgacggagta
     6121 taagcgctta gcgcaagcac atggtctgaa tttaaaacag gctaaaccaa tcaccatggg
     6181 tatgtggata ggtggtgacc gtgatggaaa tccatttgtt acagcaaaga ccttgaagca
     6241 gtctgcactc actcagtgtg aagtcatcat gaactactat gataaaaaga tttaccaact
     6301 ttatcgtgaa ttttctcttt caactagcat tgtcaacgtc agcaagcaag tcagagaaat
     6361 ggctcgtcaa tccaaggata actcgattta ccgcgaaaaa gagctttacc gtcgtgcctt
     6421 gtttgatatt caatcaaaaa ttcaggcaac taaaacctat ctgattgagg atgaagaagt
     6481 tgggactcgt tatgaaaccg ccaatgattt ctacaaggat ttgattgcca ttcgagattc
     6541 tctactagaa aataagggcg agtccttgat ttcaggtgat tttgtggaat tattgcaggc
     6601 agtagagata tttggttttt acttagcatc aattgatatg cgacaagact ctagcgtcta
     6661 tgaagcctgt gtggcagaac tcttgaaatc agcaggaatt cattctcgtt atagcgagtt
     6721 gagcgaagaa gaaaagtgtg accttctctt gaaagaatta gaagaagatc cccgaattct
     6781 ttctgcgact cacgcagaaa aatcagaatt attagcaaaa gaactagcta tttttaagac
     6841 ggctcgtgtt ttgaaagata agttgggaga tgatgtcatc cgtcagacca tcatttcaca
     6901 tgcaaccagc ctttctgata tgctagaatt agctattctg ttaaaagaag taggactggt
     6961 ggatacggaa agggcgcgtg ttcagattgt tccccttttt gaaacaattg aagacttgga
     7021 tcattcagag gaaacaatga gaaaatatct ttctcttagc cttgccaaaa aatggattga
     7081 ctcacgaaat aactaccaag aaatcatgct tggctactct gacagtaata aagatggcgg
     7141 ttacttgtca tcatgttgga ccctctacaa ggctcaacaa caattgactg ctattggaga
     7201 tgaatttggc gttaaggtta ccttcttcca tggtcgtggt ggtactgtcg gtcgtggtgg
     7261 tgggccaacc tatgaagcca ttacatctca accgctcaag tctatcaagg atcgtatccg
     7321 cttgacggag cagggtgaag taattgggaa taaatacggt aacaaagacg ccgcttacta
     7381 taaccttgaa atgctagtat cggcagctat taaccgtatg attactcaga agaagagcga
     7441 taccaatacc ccaaatcgtt atgaagccat tatggatcaa gtagtggacc gtagttacga
     7501 tatctaccgt gatttggtct ttggtaatga gcatttctat gattatttct tcgagtcaag
     7561 tccaatcaag gctatttcaa gttttaatat tggttctcgt ccagccgctc gtaagactat
     7621 tactgaaatc ggtggattgc gtgctatccc ttgggtattc tcatggtcac agagtcgtgt
     7681 tatgttccct ggatggtacg gggttggttc aagcttcaag gaatttatca ataaaaatcc
     7741 agagaatatt gctatcttac gagatatgta ccaaaattgg cctttcttcc aatcgcttct
     7801 ttcaaatgtt gatatggttt tgtcaaaatc aaatatgaat attgcttttg aatatgctaa
     7861 actttgtgaa gacgagcaag ttaaggccat ctatgagact attttaaatg aatggcaagt
     7921 tactaagaac gttatcttgg ctattgaagg acatgacgaa ctcttagctg acaatccata
     7981 tctaaaagct agtctggatt accgtatgcc ttactttaat attctcaact atattcagtt
     8041 agagttgatt aaacgccaac gtcgtggaga attgtccagt gatcaagaac gattgattca
     8101 tatcaccatc aacggaattg cgacaggatt gcgtaattca ggttgataat tttcaagagt
     8161 gaatgctaaa agtgaatatc aaaaaaattc taatagacta ttgacaagta gtttaaaaat
     8221 gatataattt aaccattcag aaaagtaatc atacaaactt tttagagagt ctgtggtagc
     8281 tgaaaacaga taagtggcaa tgatgaaaat tgggctgaat gctatttaga atttgaaatt
     8341 ataaaaattc ggtaagcaca ccttacagtg catctcgtta ttgcgagact gagcgatagg
     8401 gaaattccct ataattgagg tggtaccgcg catcgacgtc ctcacacaag ttttttgtgt
     8461 gaggattttt ttgatggagg ttagtatgga aagaaaa
//
LOCUS       DAVLVP010000078         8491 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.078, whole genome shotgun sequence.
ACCESSION   DAVLVP010000078 DAVLVP010000000
VERSION     DAVLVP010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.078"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            102..506
                     /locus_tag="VYN69_001639"
     CDS             102..506
                     /locus_tag="VYN69_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533876.1"
                     /translation="MKILKRYILELCFILSFALPFIKGANADNGRCFVETYYGFTFLM
                     EHAIVTAVFIYSFLIAFLLKKRWAKWIAAGSYCFLVLWIATEGYFFRMSLEDLIRLWT
                     SLEILTQTYQLGFYLNILLGILLIIKYFKVKQ"
     gene            722..1303
                     /locus_tag="VYN69_001640"
     CDS             722..1303
                     /locus_tag="VYN69_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000289981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEV1533877.1"
                     /translation="MYYAENPDAAHDIYELRVELLGQKMTFLTDAGVFSKKIVDFGSQ
                     LLLKCLEVNQGETVLDVGCGYGPLGLSLAKAYGVQATMVDINTRALDLARRNAEKNNA
                     KATIFQSNIYEQVEGHFDHVISNPPIRAGKQVVHEIIEKSKDFLETGGDLTIVIQKKQ
                     GAPSAKSKMEDVFGNCEILKKDKGYYILRSVKE"
     gene            1300..2577
                     /locus_tag="VYN69_001641"
     CDS             1300..2577
                     /locus_tag="VYN69_001641"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001202959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEV1533878.1"
                     /translation="MRAVDLIQKKRDGQELTSSEIEWLIEGYVSGTVPDYQMSAFAMA
                     VYFKGMTTREISDLTMNMVKTGQEFDLSAIDGVKVDKHSTGGVGDKVTLILAPLVASF
                     GVPVAKMSGRGLGHTGGTIDKLESIKGYQVERSQEDFIRQVQDIGVSVIGQSDQLVKA
                     DKLLYALRDVTATVDTIPLIASSVMSKKIAAGADAILLDVTVGEGAFMKTVDEARELA
                     QTMVDLGKVVGRKTVAVITDMSQPLGRAIGNRLEILEALEILQGQGRQDITHFICELA
                     QIMLGLANVNKTVEEVRQHLENGQALAKFEEMVQAQGGDLEDLYRPVNVAHVVEIPAQ
                     ETGVISALPAMDFGLYAMRLGAGRAVKSDALDYETGIVFEKKIGDSVQKGEIVAKVYT
                     NEKIPPQLVTDFQKCVKISDEVKKIREIVEIIS"
     gene            2595..3257
                     /gene="deoC"
                     /locus_tag="VYN69_001642"
     CDS             2595..3257
                     /gene="deoC"
                     /locus_tag="VYN69_001642"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000773666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEV1533879.1"
                     /translation="MKLNKYIDHTLLKQDAKKKQIDSLLSEAREYGFASVCVNPTWVE
                     HAKKGLEGTDVKVCTVVGFPLGATTSAVKAFETKEAIQNGADEIDMVINVGALKSGNL
                     ALVESDIRAVVEASGDKLVKVIIEACLLTDQEKVVVCQLAQKAGADFVKTSTGFSTGG
                     ATIADVTLMRETVGSDMGVKAAGGARSYADALAFVEAGATRIGTSAGVAILKGELADG
                     DY"
     gene            3244..3633
                     /locus_tag="VYN69_001643"
     CDS             3244..3633
                     /locus_tag="VYN69_001643"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009730277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEV1533880.1"
                     /translation="MATTELIELAIETSKHAYVPYSHFPIGAVLVAKDGNVYTGVNIE
                     NASYPLTNCGERTAIFKAISEGQREFSELIVYGQTEKPISPCGACRQVMVEFFEQDLK
                     VTLVAKDKSTVEMTVGELLPYSFTDLN"
     gene            3723..4775
                     /locus_tag="VYN69_001644"
     CDS             3723..4775
                     /locus_tag="VYN69_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HEV1533881.1"
                     /translation="MNKKQWLGLGLVAVAAVGLAACGNRSSRNAASSSDVKTKAAIVT
                     DTGGVDDKSFNQSAWEGLQAWGKEHNLSKDNGFTYFQSTSEADYANNLQQAAGSYNLI
                     FGVGFALHNAVKDAAKEHTDLNYVLIDDVIKDQKNVASVTFADNESGYLAGVAAAKTT
                     KTKQVGFVGGIESEVISRFEAGFKAGVASVDPSIKVQVDYAGSFGDAAKGKTIAAAQY
                     AAGADIVYQVAGGTGAGVFAEAKSLNESRPENEKVWVIGVDRDQEAEGKYTSKDGKES
                     NFVLVSTLKQVGTTVKDISNKAEKGEFPGGQVIVYSLKDKGVDLAVTNLSEEGKKAVE
                     DAKAKILDGSVKVPEK"
     gene            4919..6454
                     /locus_tag="VYN69_001645"
     CDS             4919..6454
                     /locus_tag="VYN69_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000929819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533882.1"
                     /translation="MAHENVIEMRDITKVFGGFVANDKINLHLRKGEIHALLGENGAG
                     KSTLMNMLAGLLEPTSGEIAVNGQVVNLDSPSKAASLGIGMVHQHFMLVEAFTVAENI
                     ILGSELTKNGVLDIAGASKEIKALSERYGLAVDPSAKVADISVGAQQRVEILKTLYRG
                     ADILIFDEPTAVLTPSEIDELMAIMKNLVKEGKSIILITHKLDEIRAVSDRVTVIRRG
                     KSIETVEIAGATNADLAEMMVGRSVSFKTEKQASKPKEVVLSIKDLVVNENRGVPAVK
                     NLSLDVRAGEIVGIAGIDGNGQSELIQAITGLRKVESGSIELKGDSIVGLHPRQITEL
                     SVGHVPEDRHRDGLILEMMISENIALQTYYKEPHSKNGILNYSNITSYAKKLMEEFDV
                     RAASELVPAAALSGGNQQKAIIAREIDRDPDLLIVSQPTRGLDVGAIEYIHKRLIEER
                     DNGKAVLVVSFELDEILNVSDRIAVIHDGKIQGIVSPETTNKQELGVLMAGGNLGKEK
                     SDV"
     gene            6447..7505
                     /locus_tag="VYN69_001646"
     CDS             6447..7505
                     /locus_tag="VYN69_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1533883.1"
                     /translation="MFKKLQQISVPLISVFLGILLGAIVMWIFGYDAIWGYEELFYTA
                     FGSLRGIGEIFRAMGPLVLIGLGFAVASRAGFFNVGLPGQALAGWILSGWFALSHPDM
                     PRPLMILATIVIALIAGGIVGAIPGILRAYLGTSEVIVTIMMNYIVLYVGNAFIHAFP
                     KDFMQSTDSTIRVGANATYQTPWLAELTGNSRMNIGIFFAIIAVAVIWFMLKKTTLGF
                     EIRAVGLNPHASEYAGISAKRTIILSMIISGALAGLGGAVEGLGTFQNVYVQGSSLAI
                     GFNGMAVSLLAANSPIGILFAAFLFGVLQVGAPGMNAAQVPSELVSIVTASIIFFVSV
                     HYLIERFVKPKKQVKGGK"
     gene            7508..8464
                     /locus_tag="VYN69_001647"
     CDS             7508..8464
                     /locus_tag="VYN69_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004261501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1533884.1"
                     /translation="MSIITLLPLLVSSMLIYSAPLIFTSIGGVFSERGGVVNVGLEGI
                     MVMGAFSGVVFNLEFAEQFGAATPWLSLLVAGLVGSVFSIIHAAATVHFRADHVVSGT
                     VLNLMAPALAVFLVKVLYNKGQTDNLSQTFGRFDFPVLANIPVIGDIFFKSTSLLGYL
                     AIAFSFLAWFILFKTQFGLRLRSVGEHPQAADTLGINVYKMRYLGIIISGFLGGIGGA
                     IYAQSISVNFSVTTIVGPGFIALAAMIFGKWNPIGAMLSSLFFGLSQSLAVIGSQLPF
                     LQGVPAVYLQIAPYVLTILVLAAFFGKAVAPKADGINYIKSK"
BASE COUNT         2403 a         1500 c         2012 g         2576 t
ORIGIN      
        1 aatataagcg atcaatttat tgatgaaata ggagatagat tctactgatt tataagctta
       61 taaagggggc tttttagttc acaacttata ggaggtgtac tatgaaaatc ttaaaacgtt
      121 acatattgga actctgtttt attttaagtt ttgctttacc ttttataaaa ggagccaatg
      181 cagataatgg tagatgcttt gtggaaacct attacggttt tacttttttg atggaacatg
      241 ctattgtaac agctgtcttt atctattcgt ttttaattgc tttcttacta aaaaaacgat
      301 gggcgaaatg gattgctgct ggtagttatt gctttttagt cctatggatt gcgacagagg
      361 gttatttctt ccgcatgtca ctagaagatt tgatacgact ttggacaagt ttggaaatcc
      421 tgacacaaac gtatcagttg ggcttttatc taaacatttt gttgggaatt ttgttgataa
      481 taaagtattt taaggttaag caatagaaat aataatctgc ctgattatag atgataaact
      541 ttttgttttt atctggactg tgatatttat agttctaaat ggtttattat ttataagtgt
      601 tgaaagagat tgaagaaaat ggcttcgtga ctcgctttct tcggtctttt tcgttttatt
      661 atcaatttgt aaacgattta caatttgagt caaattatgg taaaatagtg tcatgagtaa
      721 aatgtattat gcagaaaatc ctgacgctgc tcacgacatt tatgagttga gagtagagtt
      781 gttgggacaa aaaatgacct ttttaacgga tgcgggtgtt tttagcaaga aaatagttga
      841 ctttggaagt caactattgc tcaagtgtct tgaggtcaac caaggagaaa ctgtccttga
      901 tgtaggctgt ggttacggac ctttgggttt gtccttagct aaggcttatg gagttcaggc
      961 tactatggtt gatatcaaca ctcgtgcctt agatttagct cggagaaatg ctgaaaaaaa
     1021 taatgcaaaa gcgacgattt tccagtccaa catctatgaa caagttgaag gacattttga
     1081 tcatgtcatt tccaatcctc ctatccgtgc gggcaagcaa gttgttcatg aaatcattga
     1141 gaaaagtaaa gattttttgg aaactggtgg ggatttgaca atcgtcatcc agaaaaaaca
     1201 aggggctcca agtgctaagt ccaagatgga ggatgtgttt ggtaattgtg aaatccttaa
     1261 aaaagacaaa ggatactata tccttagaag tgtgaaagaa tgagagcagt tgatttaatc
     1321 caaaaaaaac gagacggtca agaactgact tcaagtgaaa ttgaatggct gatagaaggc
     1381 tatgtatcag gaactgttcc tgattatcag atgtctgcct ttgctatggc tgtttatttt
     1441 aaaggaatga cgactcgaga aatctctgat ttaacgatga atatggttaa gactgggcaa
     1501 gagtttgacc tgtcagccat tgatggtgtg aaagttgaca agcattctac tggtggtgtc
     1561 ggcgataagg taaccttgat tttagctcct cttgttgcta gcttcggtgt gcctgtagca
     1621 aaaatgagtg gtcgtggtct tggccataca ggcggaacaa ttgataaatt ggagtccatt
     1681 aagggctatc aagtagaacg tagtcaagag gattttattc gtcaggttca ggatattgga
     1741 gtatctgtca ttgggcaatc agaccagctg gtcaaagcag ataagcttct ctatgccctt
     1801 cgtgatgtga ccgcaactgt cgacacgatt cctttgattg cgagttcggt gatgagcaag
     1861 aaaattgcgg caggggcgga tgctattttg ctagacgtga ctgtcggtga gggtgccttc
     1921 atgaagacgg ttgatgaggc gcgtgagttg gctcaaacta tggtagatct tggtaaggtt
     1981 gttggacgga agacagtagc agtgattacg gatatgagcc agcctttggg acgagcgatt
     2041 ggaaatcgtc tggaaatcct tgaagcattg gagattttgc aaggtcaagg acgtcaggat
     2101 attacccact ttatttgtga attggctcaa attatgcttg gtttggcaaa tgttaacaaa
     2161 actgttgaag aagttcgcca acatcttgag aatggtcaag cactggctaa gtttgaggaa
     2221 atggtgcaag cccaaggtgg agacttggaa gacctctatc gtcctgtaaa tgtagctcat
     2281 gtggtggaaa tccctgctca ggaaacgggt gtcatttcag ctcttccagc tatggacttt
     2341 ggtctttatg ccatgaggct gggtgctggt cgtgcagtta agtctgatgc cttggactat
     2401 gaaacaggaa ttgtttttga aaagaagatt ggagactccg ttcaaaaggg tgaaattgtt
     2461 gcaaaagtat acacaaatga aaaaattcca cctcaactag ttacagattt ccaaaaatgt
     2521 gttaaaataa gtgatgaagt gaaaaaaatc cgtgaaattg tagaaatcat ctcctaaatg
     2581 gagggaaaac cgaaatgaaa ttaaataaat atatagatca tacgctttta aaacaagatg
     2641 caaagaaaaa acaaattgat agtttgttgt ctgaggctag agagtatggc tttgccagtg
     2701 tttgcgttaa tccaacctgg gttgaacatg ctaaaaaagg gcttgaaggc acagatgtta
     2761 aggtttgcac ggtagtaggt ttccctttgg gagcaacaac ttcagccgtg aaagcatttg
     2821 agacaaaaga agctatccaa aatggtgcag atgagattga tatggtgatc aatgttggag
     2881 ctctcaaatc aggtaattta gccttggttg agtcagatat tcgcgcagta gtggaagcaa
     2941 gtggtgataa gttagtgaaa gtcattattg aagcttgcct tctgacagac caagaaaaag
     3001 ttgttgtttg ccaattggcc caaaaagctg gggctgactt tgtcaaaaca tctactggct
     3061 tttcaactgg tggtgctacg atagcagatg ttacattaat gcgtgaaaca gttggatctg
     3121 atatgggagt caaggctgct ggtggagctc gttcttatgc agatgctctt gcctttgtcg
     3181 aagcaggtgc gacccgtatc ggaacgtcag ctggggtagc tattttaaaa ggagaattgg
     3241 cagatggcga ctactgagtt gattgaactg gcaattgaaa ccagcaaaca tgcctatgtc
     3301 ccctattctc actttcctat cggagcagtt ttagtagcca aagacggaaa tgtttacacg
     3361 ggagtgaata tcgaaaatgc tagctatcct ttgaccaatt gcggtgagag aacagctatt
     3421 tttaaggcta tatctgaagg ccaaagagaa ttttcagaat tgattgtcta tggtcaaact
     3481 gaaaaaccga tttcaccatg tggtgcttgt cgccaagtaa tggtcgaatt ttttgaacaa
     3541 gatttaaaag tgaccttagt cgcaaaagat aaatcgacgg tcgagatgac ggtcggggaa
     3601 ttacttccat actcttttac agacttaaac tagtctgagt cgctctctga gtggcacggt
     3661 ccttgtggcc aaccaatcca tacttgcaac atcgttgcac atcttattta ggaggttcag
     3721 taatgaacaa gaaacaatgg ctaggtcttg gcctagttgc agtggcagca gttggacttg
     3781 ctgcatgtgg taaccgctct tctcgtaacg cagcttcatc ttctgatgtg aagacaaaag
     3841 cagcaatcgt cactgatact ggtggtgttg atgacaaatc attcaaccaa tcagcttggg
     3901 aaggtttgca ggcttggggt aaagaacaca atctttcaaa agataacggt ttcacttact
     3961 tccaatcaac aagtgaagct gactacgcta acaacttgca acaagcggct ggaagttaca
     4021 acctaatctt cggtgttggt tttgcccttc ataatgcagt taaagatgca gcaaaagaac
     4081 acactgactt gaactatgtc ttgattgatg atgtgattaa agaccaaaag aatgttgcga
     4141 gcgtaacttt cgctgataat gagtcaggtt accttgcagg tgtggctgca gcaaaaacaa
     4201 ctaagacaaa acaagttggt tttgtaggtg gtatcgaatc tgaagttatc tctcgttttg
     4261 aagcaggatt caaggctggt gttgcgtcag tagacccatc tatcaaagtc caagttgact
     4321 acgctggttc atttggtgat gcggctaaag gtaaaacaat tgcagccgca caatacgcag
     4381 ccggtgcaga tattgtttac caagtagctg gtggtacagg tgcaggtgtc tttgcagagg
     4441 caaaatctct caacgaaagc cgtcctgaaa atgaaaaagt ttgggttatc ggtgttgatc
     4501 gtgaccaaga agcagaaggt aaatacactt ctaaagatgg caaagaatca aactttgttc
     4561 ttgtatctac tttgaaacaa gttggtacaa ctgtaaaaga tatttctaac aaggcagaaa
     4621 aaggagaatt ccctggcggt caagtgatcg tttactcatt gaaggataaa ggggttgact
     4681 tggcagtaac aaacctttca gaagaaggta aaaaagctgt cgaagatgca aaagctaaaa
     4741 tccttgatgg aagcgtaaaa gttcctgaaa aataatggat ggaagtcatt tcttaggaag
     4801 cggccaaggg ccgctttttt aagagttgag acaaagtcat tttgtctcag taaagcttgc
     4861 tactagacaa actagcaagt tttattgaaa taaaataaga ctctgaaagg aagagcacat
     4921 ggcacacgaa aatgtcattg agatgcgtga tattaccaag gtgtttggtg gatttgttgc
     4981 caacgacaaa atcaacttgc acctacgaaa aggtgaaatt catgcacttt taggagaaaa
     5041 tggggctggt aagtccacgc taatgaacat gttagcaggc cttcttgaac caactagtgg
     5101 tgaaatcgcg gtcaacggtc aagttgtcaa tctcgactcc ccatctaaag cagctagctt
     5161 gggaatcggg atggttcacc agcactttat gttggttgaa gccttcacag tggctgaaaa
     5221 catcatttta ggtagtgaat tgactaaaaa tggtgtgcta gatatcgctg gagctagcaa
     5281 agaaatcaag gctctttctg aacgttatgg cttagctgtt gacccttctg ccaaggtagc
     5341 agatatctca gttggagccc aacaacgtgt agaaatttta aaaacacttt atcggggggc
     5401 tgatatcctt atctttgacg aaccaacggc tgttttgact ccatcagaaa ttgatgagtt
     5461 gatggctatt atgaaaaatc ttgtcaaaga aggaaaatca attatcttga ttacccacaa
     5521 attggatgaa attcgcgcag tttctgaccg tgttacagtt atccgtcgtg ggaaatcaat
     5581 tgaaaccgtt gaaattgcag gggctaccaa tgctgatttg gcggaaatga tggtaggacg
     5641 ttctgtttcc tttaaaacag agaagcaagc ctctaaacca aaagaagtgg ttttgtctat
     5701 caaagatttg gtggtcaatg aaaaccgtgg tgttccagct gttaaaaatc tgtccttgga
     5761 tgttcgtgct ggagagattg ttggtattgc ggggattgat ggaaatggtc agtctgaact
     5821 gattcaagcc attacaggtc ttcgtaaggt tgaatctggt agcattgagc taaaagggga
     5881 ttcaattgta ggcttgcacc cacgtcagat tacagaacta agtgttgggc acgttccaga
     5941 agaccgtcac cgtgatggct tgattttgga aatgatgata tctgaaaata ttgcccttca
     6001 aacctactat aaagaaccac atagtaaaaa tggaattttg aattattcaa atattacttc
     6061 ttatgctaaa aagctgatgg aagagtttga tgttcgcgct gccagtgaat tagttcctgc
     6121 agctgcactc tcaggaggaa atcaacaaaa agcaattatt gctcgtgaaa ttgatcgaga
     6181 tcctgatctc cttatcgtta gccagccaac tcgtgggttg gatgtcggtg ccattgagta
     6241 tatccacaaa cgcttgattg aagagcgtga taatggcaag gctgtccttg ttgtcagctt
     6301 tgaattggat gagattttaa acgtctcaga ccgtattgcc gttatccacg atggtaagat
     6361 tcaaggtatt gtatcaccag aaacaaccaa taaacaagaa cttggtgtct tgatggctgg
     6421 tggaaacttg ggaaaggaga agagtgatgt ttaaaaaatt acaacaaatt tcggttccct
     6481 tgatttctgt atttctagga attttactcg gagccattgt catgtggatc ttcggttatg
     6541 atgctatttg gggctacgaa gaattgttct atacagcctt tggcagtctg cgtgggattg
     6601 gagaaatctt ccgtgctatg ggtcctctgg tcttgattgg tcttggtttt gcggttgcca
     6661 gtcgagctgg tttctttaac gtcggacttc ctggtcaggc tttggcaggt tggattctca
     6721 gtggttggtt tgcgctgtcg catccagata tgccccgtcc cttgatgatt ctagcaacca
     6781 tcgtgattgc cttgattgct ggtgggattg tcggagcgat tccaggtatt cttagggcct
     6841 atctagggac gtcagaggtt attgtaacca tcatgatgaa ctacattgtc ttgtatgtag
     6901 ggaatgcctt tatccatgct ttccctaaag acttcatgca aagtacagat tcgaccattc
     6961 gtgttggggc taatgcaacc tatcagacac cttggttggc tgagttgact ggtaactcac
     7021 ggatgaatat tggtattttc tttgccatca ttgccgttgc agttatttgg ttcatgctca
     7081 agaaaacaac tcttggtttt gaaatccgtg cagttggtct taatccacat gcttcagaat
     7141 atgctggtat ttctgccaag cggactatta tcctatctat gattatttca ggtgccttgg
     7201 caggtcttgg tggagctgtt gaaggtttgg gaaccttcca gaacgtctat gttcaaggtt
     7261 cgtcattagc tatcggattt aacggaatgg cggttagttt gcttgcggcc aactcaccaa
     7321 ttggtatact ctttgcagcc ttcctatttg gcgttctcca agttggggct cctggtatga
     7381 atgcggcgca ggtaccatct gagcttgtca gcattgtaac agcgtctatt atcttctttg
     7441 tcagtgttca ttaccttatc gaacgctttg ttaaaccgaa aaaacaagtt aaaggaggta
     7501 agtaaagatg tctattataa ccttgctccc cctcttggtg tcttctatgc tgatttactc
     7561 agcacctctc atctttacaa gtatcggtgg tgttttctct gaacgtggtg gtgtggtaaa
     7621 cgttggcctt gaaggaatta tggttatggg tgccttttct ggagttgtct ttaaccttga
     7681 atttgcagaa caatttggag cagcaactcc atggctatcc ttgcttgtag caggattggt
     7741 tggtagtgtt ttctctatca tccacgcagc agcgacggtt catttccgtg cagaccatgt
     7801 tgtcagcggt acggtattga acttgatggc gcctgccttg gctgttttct tagttaaagt
     7861 tctttataac aaaggacaaa ccgacaacct aagtcaaact tttggacgct ttgatttccc
     7921 agtcttggca aatatcccag tgattggtga tatcttcttc aagtcaacta gtctacttgg
     7981 ttatctggcg attgccttct cattccttgc ttggtttatt ctctttaaga ctcaatttgg
     8041 tcttcgtctc cgctctgtcg gtgaacatcc tcaagcagcg gataccttgg gaatcaacgt
     8101 ctacaagatg agatatttag ggattattat ttcaggtttt ctaggtggaa ttggcggagc
     8161 gatttatgcg caatcaatct cagttaactt ctcagtgaca actattgttg gacctggatt
     8221 tatcgccctt gctgcgatga tctttgggaa atggaatcca atcggtgcta tgctttctag
     8281 tctcttcttt ggactttcac aaagtttggc tgttatcggt tctcaattgc cgttcctaca
     8341 aggagtgcca gcggtttatc ttcaaattgc accttatgtt ttgacaattc ttgtcttagc
     8401 agccttcttt ggaaaagcag tcgcaccaaa agcagatggt atcaactaca tcaaatcaaa
     8461 ataagcatac aaaaaaacgt cagttcaatg a
//
LOCUS       DAVLVP010000079         8442 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.079, whole genome shotgun sequence.
ACCESSION   DAVLVP010000079 DAVLVP010000000
VERSION     DAVLVP010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8442)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8442)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8442
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.079"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..2344
                     /gene="mfd"
                     /locus_tag="VYN69_001648"
     CDS             <1..2344
                     /gene="mfd"
                     /locus_tag="VYN69_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1533885.1"
                     /translation="KMDYTIILQSSNSMGSKTLEDMLEEYQIKLDSRDKTSICKESVN
                     LIEGNLRHGFHFVDEKILLITEHEIFQKKLKRRFRRQHVSNAERLKDYNELEKGDYVV
                     HHIHGIGQYLGIETIEIKGIHRDYVSVQYQNGDQISIPVEQIHLLSKYISSDGKAPKL
                     NKLNDGHFKKAKQKVKNQVEDIADDLIKLYSERSQLKGFAFSADDDDQDAFDDAFPYV
                     ETDDQLRSIEEIKRDMQASQPMDRLLVGDVGFGKTEVAMRAAFKAVNDHKQVVILVPT
                     TVLAQQHYTNFKERFQNFAVNIDVLSRFRSKKEQTATLEKLKNGQVDILIGTHRVLSK
                     DVVFADLGLMIIDEEQRFGVKHKETLKELKKQVDVLTLTATPIPRTLHMSMLGIRDLS
                     VIETPPTNRYPVQTYVLEKNDSVIRDAVLREMERGGQVYYLYNKVDTIVQKVSELQEL
                     IPEASIGYVHGRMSEVQLENTLLDFIEGQYDILVTTTIIETGVDIPNANTLFIENADH
                     MGLSTLYQLRGRVGRSNRIAYAYLMYRPEKSISEVSEKRLEAIKGFTELGSGFKIAMR
                     DLSIRGAGNLLGKSQSGFIDSVGFELYSQLLEEAIAKRNGNANANTRTKGNAELILQI
                     DAYLPDTYISDQRHKIEIYKKIRQIDNRVNYEELQEELIDRFGEYPDVVAYLLEIGLV
                     KSYLDKVFVQRVERKDNKRKDNKITIQFEKVTQRLFLAQDYFKALSVTNLKAGIAENK
                     GLMELVFDVQNKKDYEILEGLLIFGESLLEIKEFKEENSI"
     gene            2402..2668
                     /locus_tag="VYN69_001649"
     CDS             2402..2668
                     /locus_tag="VYN69_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEV1533886.1"
                     /translation="MRLDKYLKVSRIIKRRTVAKEVADKGRIKVNGILAKSSTDLKVN
                     DQVEIRFGNKLLLVKVLEMKDSTKKEDTAGMYEIISETRVEENV"
     gene            2661..3029
                     /locus_tag="VYN69_001650"
     CDS             2661..3029
                     /locus_tag="VYN69_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEV1533887.1"
                     /translation="MSKNIVQLNNSFIQNEYQRRRYLMKERQKRNRFMGGVLILIMLL
                     FILPTFNLAQSYQQLLQRRQQLADLQTQYQTLSDEKDKETAFATKLKDEDYAAKYTRA
                     KYYYSKSREKVYTIPDLLQR"
     gene            3149..4417
                     /locus_tag="VYN69_001651"
     CDS             3149..4417
                     /locus_tag="VYN69_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase-related serine hydrolase"
                     /protein_id="HEV1533888.1"
                     /translation="MRKFLIILLLPSFLTISKVVSTEKEVVYTSKEIYYLSQSDFGIY
                     FREKLSSPMVYGEVPVYANEDLVVESGKLTPKTSFQITEWRLNKQGIPVFKLSNHQFI
                     AADKRFLYDQSEVTPTIKKVWLESDFKLYNSPYDLKEVKSSLSAYSQVSIDKTMFVEG
                     REFLHIDQAGWVAKESTSEEDNRMSKVQEMLSEKYQKDSFSIYVKQLTTGKEAGINQD
                     EKMYAASVLKLSYLYYTQEKINEGLYQLDTTVKYVSAVNDFPGSYKPEGSGSLPKKED
                     NKEYSLKDLITKVSKESDNVAHNLLGYYISNQSDATFKSKMSAIMGDDWDPKEKLISS
                     KMAGKFMEAIYNQNGFVLESLTKTDFDSQRIAKGVSVKVAHKIGDADEFKHDTGVVYA
                     DSPFILSIFTKNSDYDTISKIAKDVYEVLK"
     gene            4414..5691
                     /gene="tilS"
                     /locus_tag="VYN69_001652"
     CDS             4414..5691
                     /gene="tilS"
                     /locus_tag="VYN69_001652"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEV1533889.1"
                     /translation="MREPDFLNHFLKKGYFKKHAKAVLALSGGLDSMFLFKVLSTYQK
                     ELEIELILAHVNHKQRIESDWEEKELRKLAAEAELPIYISNFSGEFSEARARNFRYDF
                     FQEVMKKTGATALVTAHHADDQVETILMRLIRGTRLRYLSGIKEKQVVGEIEIIRPFL
                     HFQKKDFPSIFHFEDTSNQENHYFRNRIRNSYLPELEKENPRFRNAILGIGNEILDYD
                     LAIAELSNNINVEDLQQLFSYSESTQRVLLQTYLNRFPDLNLTKAQFAEVQQILKSKS
                     QYRHPIKNGYELIKEYQQFQICKISPQADEEEDEFVLHYQNQVAYQGYLFSFGLPLEG
                     ESIQQIPVSRETSIHIRHRKTGDVLIQNGHRKKLRRLFIDLKIPMEKRNSALIIEQFG
                     EIVSILGIATNNLSKKTKNDIMNTVLYIEKIDR"
     gene            5695..6237
                     /gene="hpt"
                     /locus_tag="VYN69_001653"
     CDS             5695..6237
                     /gene="hpt"
                     /locus_tag="VYN69_001653"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEV1533890.1"
                     /translation="MLENDIKKVLVSHDEITEAAKKLGAQLTKDYAGKNPILVGILKG
                     SIPFMAELVKHIDTHIEMDFMMVSSYHGGTASSGVINIKQDVTQDIKGRHVLFVEDII
                     DTGQTLKNLRDMFKEREAASVKIATLLDKPEGRVVEIEADYTCFTIPNEFVVGYGLDY
                     KENYRNLPYIGVLKEEVYSN"
     gene            6253..8211
                     /gene="ftsH"
                     /locus_tag="VYN69_001654"
     CDS             6253..8211
                     /gene="ftsH"
                     /locus_tag="VYN69_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEV1533891.1"
                     /translation="MKKQNNGLIKNPFLWLLFIFFLVTGFQYFYSGNNSGGSQQINYT
                     ELVQEITDGNVKELTYQPNGSVIEVSGVYKNPKTSKEGTGIQFFTPSVTKVEKFTSTI
                     LPADTTVSELQKLATDHKAEVTVKHESSSGIWINLLVSIVPFGILFFFLFSMMGNMGG
                     GNGRNPMSFGRSKAKAANKEDIKVRFSDVAGAEEEKQELVEVVEFLKDPKRFTKLGAR
                     IPAGVLLEGPPGTGKTLLAKAVAGEAGVPFFSISGSDFVEMFVGVGASRVRSLFEDAK
                     KAAPAIIFIDEIDAVGRQRGVGLGGGNDEREQTLNQLLIEMDGFEGNEGIIVIAATNR
                     SDVLDPALLRPGRFDRKVLVGRPDVKGREAILKVHAKNKPLAEDVDLKLVAQQTPGFV
                     GADLENVLNEAALVAARRNKSIIDASDIDEAEDRVIAGPSKKDKTVSQKERELVAYHE
                     AGHTIVGLVLSNARVVHKVTIVPRGRAGGYMIALPKEDQMLLSKEDMKEQLAGLMGGR
                     VAEEIIFNVQTTGASNDFEQATQMARAMVTEYGMSEKLGPVQYEGNHAMLGAQSPQKS
                     ISEQTAYEIDEEVRSLLNEARNKAAEIIQSNRETHKLIAEALLKYETLDSTQIKALYE
                     TGKMPEAVEEESHALSYDEVKSKMNDEK"
BASE COUNT         2905 a         1240 c         1766 g         2531 t
ORIGIN      
        1 aaaaatggat tacaccatta ttctgcagtc tagcaattca atgggaagta aaacattgga
       61 ggatatgtta gaggaatatc agattaaatt ggattctaga gataagacaa gtatctgtaa
      121 agaatctgta aacttaatag agggtaatct cagacatggt tttcattttg tagatgaaaa
      181 gattttattg ataactgaac atgagatttt tcaaaagaaa ttaaagcgtc gttttcgaag
      241 acaacatgtt tcaaatgcag agagattaaa agattacaat gagcttgaaa aaggggacta
      301 tgttgtccat catatccatg ggattggtca atatctagga attgaaacca ttgaaatcaa
      361 gggaattcat cgcgattatg tcagtgtcca ataccaaaat ggtgatcaaa tttctatccc
      421 cgtggaacag attcatctac tgtccaaata tatttcaagt gatggtaaag ctccaaaact
      481 caataaatta aatgacggtc attttaaaaa ggccaagcaa aaggttaaga accaggtaga
      541 ggatatagct gatgatttaa tcaaactcta ctctgaacgt agtcagttga agggttttgc
      601 tttctcagct gatgatgatg atcaagatgc ctttgatgat gctttccctt atgttgaaac
      661 ggatgatcaa cttcgtagta ttgaggaaat caagagggat atgcaggctt ctcagccaat
      721 ggatcgactt ttagttgggg atgttggttt tggaaagact gaagttgcta tgcgtgcagc
      781 ctttaaagca gtcaatgatc acaaacaggt tgtcattcta gttccgacga cggttttagc
      841 gcaacagcac tatacgaatt ttaaggaacg attccaaaat tttgcagtta atattgatgt
      901 gttgagtcgc tttagaagta aaaaagagca gactgcaaca cttgaaaaat tgaaaaacgg
      961 tcaagtcgat attttgattg gaacacatcg tgttttgtca aaagatgttg tgtttgctga
     1021 tttgggcttg atgattattg atgaggaaca gcgatttggt gtcaagcata aggaaacttt
     1081 gaaagaactg aagaaacaag tggatgtcct aaccttgacc gctacgccaa tccctcgtac
     1141 cctccatatg tctatgctgg gaatcagaga tttatctgtt attgaaactc cgccgactaa
     1201 tcgctatcct gttcagacct atgttttgga aaagaatgat agtgtcattc gtgatgctgt
     1261 cttgcgtgaa atggagcgtg gaggtcaagt ttattatctt tacaacaaag ttgacacaat
     1321 tgttcagaag gtttcagaat tacaggagtt gattccggag gcttcgattg gatatgttca
     1381 tggtcgaatg agtgaagtcc agttggaaaa tactctatta gactttattg agggacaata
     1441 cgatatcttg gtgacgacta ctattattga gacaggggtg gacattccaa atgctaatac
     1501 tttatttatt gaaaatgcgg accatatggg cttgtcaacc ttatatcagt taagaggaag
     1561 agtcggtcgt agtaatcgta ttgcttatgc ttatctcatg tatcgtccag aaaaatcaat
     1621 cagtgaagtc tctgaaaaga gattagaagc gattaaagga tttacagaat tgggctctgg
     1681 ctttaagatt gcaatgcgag atctttcgat tcgtggagca ggaaatcttt taggaaaatc
     1741 ccagtctggt ttcattgatt ctgttggttt tgaattgtat tcgcagttat tagaggaagc
     1801 tattgctaaa cgaaacggta atgctaacgc taacacaaga accaaaggga atgctgagtt
     1861 gattttgcaa attgatgcct atcttcctga tacttatatt tctgatcaac gacataagat
     1921 tgaaatttac aagaaaattc gtcaaattga caaccgtgtc aattatgaag agttacaaga
     1981 ggagttgata gaccgttttg gagaataccc agatgtagta gcctatcttt tagagattgg
     2041 tttggttaaa tcatacttgg acaaggtctt tgttcaacgt gtggaaagaa aagataataa
     2101 aagaaaagat aataaaatta caattcaatt tgaaaaagtc actcaacgat tgtttttagc
     2161 tcaagattat tttaaagctt tatccgtaac gaacttaaaa gcaggcatcg ctgagaataa
     2221 gggattaatg gagcttgtat ttgatgtcca aaataagaaa gattatgaaa ttttagaagg
     2281 tctgctgatt tttggagaaa gtttattaga gataaaagag tttaaggaag aaaattccat
     2341 ttgatatttt tcttctataa aatagataaa aatggtacaa taataaattg aggtaataag
     2401 gatgagatta gataaatatt taaaagtatc gcgaattatc aagcgtcgta cagtcgcaaa
     2461 ggaagtagca gataaaggta gaatcaaggt taatggaatc ttggccaaaa gttcaacgga
     2521 cttgaaagtt aatgaccaag ttgaaattcg ctttggcaat aagttgctgc ttgtaaaagt
     2581 actagagatg aaagatagta caaaaaaaga agatacagca ggaatgtatg aaattatcag
     2641 tgaaacacgg gtagaagaaa atgtctaaaa atattgtaca attgaataat tcttttattc
     2701 aaaatgaata ccaacgtcgt cgctacctga tgaaagaacg acaaaaacgg aatcgtttta
     2761 tgggaggggt attgattttg attatgctat tatttatctt gccaactttt aatttagcgc
     2821 agagttatca gcaattactc caaagacgtc agcaattagc agacttgcaa actcagtatc
     2881 aaactttgag tgatgaaaag gataaggaga cagcatttgc taccaagttg aaagatgaag
     2941 attatgctgc taaatataca cgagcgaagt actattattc taagtcgagg gaaaaagttt
     3001 atacgattcc tgacttgctt caaaggtgat aaaatggaaa atttattaga cgtaatagag
     3061 caatttttga gtttgtcaga tgaaaagctg gaagaattgg ctgataaaaa tcaattattg
     3121 cgtttacaag aagaaaagga aaggaagaat gcgtaaattc ttaattattt tgttgttacc
     3181 aagttttttg accatttcaa aagtcgttag cacagaaaaa gaagtcgtct atacttcgaa
     3241 agaaatttat tacctttcac aatctgactt tggtatttat tttagagaaa aattaagttc
     3301 tcccatggtt tatggagagg ttcctgttta tgcgaatgaa gatttagtag tggaatctgg
     3361 gaaattgact cccaaaacaa gttttcaaat aaccgagtgg cgcttaaata aacaaggaat
     3421 tccagtattt aagctatcaa atcatcaatt tatagctgcg gacaaacgat ttttatatga
     3481 tcaatcagag gtaactccaa caataaaaaa agtatggtta gaatctgact ttaaactgta
     3541 caatagtcct tatgatttaa aagaagtgaa atcatcctta tcagcttatt cgcaagtatc
     3601 aatcgacaag accatgtttg tagaaggaag agaatttcta catattgatc aggctggatg
     3661 ggtagctaaa gaatcaactt ctgaagaaga taatcggatg agtaaagttc aagaaatgtt
     3721 atctgaaaaa tatcagaaag attctttctc tatttatgtt aagcaactga ctactggaaa
     3781 agaagctggt atcaatcaag atgaaaagat gtatgcagcc agcgttttga aactctccta
     3841 tctctattat acgcaagaaa aaataaatga gggtctttat cagttagata cgactgtaaa
     3901 atacgtatct gcagtcaatg attttccagg ttcttataaa ccagagggaa gtggtagtct
     3961 tcctaaaaaa gaagataata aagaatattc tttaaaggat ttaattacga aagtatcaaa
     4021 agaatctgat aatgtagctc ataatctatt gggatattac atttcaaacc aatctgatgc
     4081 cacattcaaa tccaagatgt ctgccattat gggagatgat tgggatccaa aagaaaaatt
     4141 gatttcttct aagatggccg ggaagtttat ggaagctatt tataatcaaa atggatttgt
     4201 gctagagtct ttgactaaaa cagattttga tagtcagcga attgccaaag gtgtttctgt
     4261 taaagtagct cataaaattg gagatgcgga tgaatttaag catgatacgg gtgttgtcta
     4321 tgcagattct ccatttattc tttctatttt cactaagaat tctgattatg atacgatttc
     4381 taagatagcc aaggatgttt atgaggttct aaaatgaggg aaccagattt tttaaatcat
     4441 tttctcaaga agggatattt caaaaagcat gctaaggcgg ttctagctct ttctggtgga
     4501 ttagattcca tgtttctatt taaggtattg tctacttatc aaaaagagtt agagattgaa
     4561 ttgattctag ctcatgtgaa tcataagcag agaattgaat cagattggga agaaaaggaa
     4621 ttaaggaagt tggctgctga agcagagctt cctatttata tcagcaattt ttcaggagaa
     4681 ttttcagaag cgcgtgcacg aaattttcgt tatgattttt ttcaagaggt catgaaaaag
     4741 acaggtgcga cagctttagt cactgcccac catgctgatg atcaggtgga aacgattttg
     4801 atgcgcttga ttcgaggaac tcgcttgcgc tatctatcag gaattaagga gaagcaagta
     4861 gtcggagaga tagaaatcat tcgtcccttc ttgcattttc agaaaaaaga ctttccatca
     4921 atttttcact ttgaagatac atcaaatcag gagaatcatt attttcgaaa tcgtattcga
     4981 aattcttact taccagaatt ggaaaaagaa aatcctcgat ttaggaatgc aatcttaggc
     5041 attggcaatg aaattttaga ttatgatttg gcaatagctg aattatctaa caatattaat
     5101 gtggaagatt tacagcagtt attttcttac tctgagtcta cacaaagagt tttacttcaa
     5161 acttatctga atcgttttcc agatttgaat cttacaaaag ctcagtttgc tgaagttcag
     5221 cagattttaa aatctaaaag ccagtatcgt catccgatta aaaatggcta tgaattgata
     5281 aaagagtacc aacagtttca gatttgtaaa atcagtccgc aggctgatga agaggaagat
     5341 gaatttgtgt tacactatca aaatcaggta gcttatcaag gatatttatt ttcctttgga
     5401 cttccattag aaggtgaatc aattcaacaa atacctgttt cacgtgaaac atccatacac
     5461 attcgtcatc gaaaaacagg agatgttttg attcaaaatg ggcatagaaa aaaactcaga
     5521 cgtttattta ttgatttgaa aatccctatg gaaaagagaa actctgctct tattattgag
     5581 caatttggtg aaattgtctc aattttggga attgcgacca ataatttgag taaaaaaacg
     5641 aaaaatgata taatgaacac tgtactttat atagaaaaaa tagataggta aaaaatgtta
     5701 gaaaacgata ttaaaaaagt cctcgtttca cacgatgaaa ttacagaagc agctaaaaaa
     5761 ctaggtgctc aattaactaa agactatgca ggaaaaaatc caatcttagt tgggatttta
     5821 aaaggatcta ttccttttat ggctgaattg gtcaaacata ttgatacaca tattgaaatg
     5881 gacttcatga tggtttctag ctaccatggt ggaacagcaa gtagtggtgt tatcaatatt
     5941 aaacaagatg tgactcaaga tatcaaagga agacatgttc tatttgtaga agatatcatt
     6001 gatacaggtc aaactttgaa gaatttgcga gatatgttta aagaaagaga agcagcttct
     6061 gttaaaattg caaccttgtt ggataaacca gaaggacgtg ttgtagaaat tgaggcagac
     6121 tatacctgct ttactatccc aaatgagttt gtagtaggtt atggtttaga ctacaaagaa
     6181 aattatcgta atcttcctta tattggagta ttgaaagagg aagtgtattc aaattagaaa
     6241 gaataatctt taatgaaaaa acaaaataat ggtttaatta aaaatccttt tctatggtta
     6301 ttatttatct ttttccttgt gacaggattc cagtatttct attctgggaa taactcagga
     6361 ggaagtcagc aaatcaacta tactgagttg gtacaagaaa ttaccgatgg taatgtaaaa
     6421 gaattaactt accaaccaaa tggtagtgtt atcgaagttt ctggtgtcta taaaaatcct
     6481 aaaacaagta aagaaggaac aggtattcag tttttcacgc catctgttac taaggtagag
     6541 aaatttacca gcactattct tcctgcagat actaccgtat cagaattgca aaaacttgct
     6601 actgaccata aagcagaagt aactgttaag catgaaagtt caagtggtat atggattaat
     6661 ctactcgtat ccattgtgcc atttggaatt ctattcttct tcctattctc tatgatggga
     6721 aatatgggag gaggcaatgg ccgtaatcca atgagttttg gacgtagtaa ggctaaagca
     6781 gcaaataaag aagatattaa agtaagattt tcagatgttg ctggagctga ggaagaaaaa
     6841 caagaactag ttgaagttgt tgagttctta aaagatccaa aacgattcac aaaacttgga
     6901 gcccgtattc cagcaggtgt tcttttggag ggacctccgg ggacaggtaa gactttgctt
     6961 gctaaggcag tcgctggaga agcaggtgtt ccattcttta gtatctcagg ttctgacttt
     7021 gtagaaatgt ttgtcggagt tggagctagt cgtgttcgct ctctttttga ggatgccaaa
     7081 aaagcagcac cagctatcat ctttatcgat gaaattgatg ctgttggacg tcaacgtgga
     7141 gtcggtctcg gcggaggtaa tgacgaacgt gaacaaacct tgaaccaact tttgattgag
     7201 atggatggtt ttgagggaaa tgaagggatt atcgtcatcg ctgcgacaaa ccgttcagat
     7261 gtacttgatc ctgccctttt gcgtccagga cgttttgata gaaaagtatt ggttggccgt
     7321 cctgatgtta aaggtcgtga agcaatcttg aaagttcacg ctaagaacaa gcctttagca
     7381 gaagatgttg atttgaaatt agtggctcaa caaactccag gctttgttgg tgctgattta
     7441 gagaatgtct tgaatgaagc agctttagtt gctgctcgtc gcaataaatc gataattgat
     7501 gcttcagata ttgatgaagc agaagataga gttattgctg gaccttctaa gaaagataag
     7561 acagtttcac aaaaagaacg agaattggtt gcttaccatg aggcaggaca taccattgtt
     7621 ggtctagtct tgtcgaatgc tcgcgttgtc cataaggtta caattgtacc acgcggccgt
     7681 gcaggcggat acatgattgc acttcctaaa gaggatcaaa tgcttctatc taaagaagat
     7741 atgaaagagc aattggctgg cttaatgggt ggacgtgtag ctgaagaaat tatctttaat
     7801 gtccaaacta caggagcttc aaacgacttt gaacaagcga cacaaatggc gcgtgcaatg
     7861 gttacagagt acggtatgag tgaaaaactt ggcccagtac aatatgaagg aaaccatgct
     7921 atgcttggtg cacagagtcc tcaaaaatca atttcagaac aaacagctta tgaaattgat
     7981 gaagaggttc gttcattatt aaatgaggca cgaaataaag ctgctgaaat tattcagtca
     8041 aatcgtgaaa ctcacaagtt aattgcagaa gcattattga aatacgaaac attggatagt
     8101 acacaaatta aagctcttta cgaaacagga aagatgcctg aagcagtaga agaggaatct
     8161 catgcactat cctatgatga agtaaagtca aaaatgaatg acgaaaaata accctgagag
     8221 aggctggagc ctctcttttt tgtgcagttt aggagctaaa gggaacagaa tggagaaaat
     8281 gtaacaaatg tgttttctaa tctgttagac tgtatctaga aaggggaaaa ttatgattaa
     8341 agaattgtat gaagaagtcc aagggactgt gtataagtgt agaaatgaat attaccttca
     8401 tttatgggaa ttgtcggatt gggaccaaga aggcatgctc tg
//
LOCUS       DAVLVP010000080         8274 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.080, whole genome shotgun sequence.
ACCESSION   DAVLVP010000080 DAVLVP010000000
VERSION     DAVLVP010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8274)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8274)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8274
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.080"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(124..327)
                     /locus_tag="VYN69_001655"
     CDS             complement(124..327)
                     /locus_tag="VYN69_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1533892.1"
                     /translation="MIKLYSPYAFLSKYLGAILLSFFCYYAKEIGCNNLTLSVWNDNE
                     GALRFYQRQGMKPQETTMEMIID"
     gene            complement(320..1402)
                     /locus_tag="VYN69_001656"
     CDS             complement(320..1402)
                     /locus_tag="VYN69_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000040939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEV1533893.1"
                     /translation="MSKLQQILTYLESEKLDVAVVSDPVTINYLTGFYSDPHERQMFL
                     FVLADQEPLLFVPALEVERASSTVSFPVVGYVDSENPWQKIKHALPQLDFKRVAVEFD
                     NLILTKYHGLKTVFETAEFDNLTPRIQRMRLIKSADEVQKMMVAGLYADKAVHVGFDN
                     ISLDKTETDIIAQIDFAMKREGYEMSFDTMVLTGDNAANPHGIPAANKVENDALLLFD
                     LGVLVNGYASDMTRTVAVGKPDQFKKDIYNLTLEAQQAALDFIKPGVTAHEVDRAARE
                     VIEKAGYGEYFNHRLGHGIGMDVHEFPSIMEGNDMVIEEGMCFSVEPGIYIPGKVGVR
                     IEDCGVVTKDGFNLFTSTSKDLLYFD"
     gene            complement(1528..2310)
                     /locus_tag="VYN69_001657"
     CDS             complement(1528..2310)
                     /locus_tag="VYN69_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEV1533894.1"
                     /translation="MVYTSLSSKDGNYPYQLNIAHLYGNLMNTYGDNGNILMLKYVAE
                     KLGAHVTVDIVSLHDDFDENHYDIAFFGGGQDFEQSIIADDLPAKKESIDNYIQNDGV
                     VLAICGGFQLLGQYYVEASGKRIEGLGVMGHYTLNQTNNRFIGDIKIHNEDFDETYYG
                     FENHQGRTFLSDDQKPLGQVVYGNGNNEEKVGEGVHYKNVFGSYFHGPILSRNANLAY
                     RLVTTALKKKYGQDIQLPAYEDILSQEIAEEYSDVKSKADFS"
     gene            complement(2310..3653)
                     /locus_tag="VYN69_001658"
     CDS             complement(2310..3653)
                     /locus_tag="VYN69_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011836724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEV1533895.1"
                     /translation="MNLKTTLGLLAGRSSHFVLSRLGRGSTLPGKVALQFDKDILQNL
                     AKNYEIVVVTGTNGKTLTTALTVGILKEVYGQVLTNPSGANMITGIATTFLTAKSSKT
                     GKNIAVLEIDEASLSRICDYIQPSLFVITNIFRDQMDRFGEIYTTYNMILDAIRKVPT
                     ATVLLNGDSPLFYKPTIPNPIEYFGFDLEKGPAQLAHYNTEGILCPDCQGILKYEHNT
                     YANLGAYICEGCGCKRPDLDYRLTKLVELTNNRSRFVIDGQEYGIQIGGLYNIYNALA
                     AVAIARFLGADSQLIKQGFDKSRAVFGRQETFHIGDKECTLVLIKNPVGATQAIEMIK
                     LAPYPFSLSVLLNANYADGIDTSWIWDADFEQITDMDIPEINAGGVRHSEIARRLRVT
                     GYPAEKITETSNLEQVLKTIENQDCKHAYILATYTAMLEFRELLASRQIVRKEMN"
     gene            complement(3765..5081)
                     /locus_tag="VYN69_001659"
     CDS             complement(3765..5081)
                     /locus_tag="VYN69_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000958936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEV1533896.1"
                     /translation="MLTYDLIVIGFGKAGKTLAGKLASAGKKVALVERSKAMYGGTCI
                     NIGCIPTKTLLVAAEKDLSFEEVIATKNTITGRLNGKNYTTVAGTGVDIFDAEAHFLS
                     NKVIEIQAGDEKQELTAETIVINTGAVSNVLPIPGLATSKNVFDSTGIQSLDKLPEKL
                     GVLGGGNIGLEFAGLYNKLGSKVTVLDALDTFLPRAEPSIAALAKQYMEEDGIELLQN
                     IHTTEIKNDGDQVLVVTEDETYRFDALLYATGRKPNVEPLQLENTDIELTERGAIKVD
                     KHCQTNVPGVFAVGDVNGGLQFTYISLDDFRVVYSYLAGDGSYTLEDRLNVPNTMFIT
                     PALSQVGLTESQAADLKLPYAVKEIPVAAMPRGHVNGDLRGAFKAVVNTETKEILGAS
                     IFSEGSQEIINIITVAMDNKIPYTYFTKQIFTHPTLAENLNDLFAI"
     gene            5275..6501
                     /locus_tag="VYN69_001660"
     CDS             5275..6501
                     /locus_tag="VYN69_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000841575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HEV1533897.1"
                     /translation="MKSNRYIIAFAGVILHLMLGSTYAWSVYRNPIIEKTGWDQASVA
                     FAFSLAIFCLGLSAAFMGRLVEKFGPRVMGSLSAFLYAGGNILTGFAIDRQELWLLYL
                     AYGILGGLGLGAGYITPVSTIIKWFPDKRGLATGLAIMGFGFASLLTSPIAQHLIAGV
                     GLVETFYILGASYFIIMLLASQFIKRPNEQELAILSASGKEKTDSLTQGMTANQALKS
                     NRFYILWIIFFINIACGLGLISAASPMAQEMAGLSTSHAAVMVGVLGIFNGFGRLLWA
                     SLSDYIGRPLTFSILLLVNLFFSLSLWLFTDSVLFVVAMSILMTCYGAGFSLIPAYLS
                     DIFGTKELAALHGYILTAWAMAGLAGPILLAETYKMAHSYTQTLFVFLILYSIALALS
                     YYLGRSIKKESQKPLT"
     gene            6700..8274
                     /locus_tag="VYN69_001661"
     CDS             6700..8274
                     /locus_tag="VYN69_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEV1533898.1"
                     /translation="MKFNELNLSADLLAEIEKAGFVEASPIQEQTIPLALEGKDVIGQ
                     AQTGTGKTAAFGLPTLEKIRTEEATIQALVIAPTRELAVQSQEELFRFGRSKGVKVRS
                     VYGGSSIEKQIKALKSGAHIVVGTPGRLLDLIKRKALKLQNIETLILDEADEMLNMGF
                     LEDIEAIISRVPENRQTLLFSATMPDAIKRIGVQFMKAPEHVKIAAKELTTELVDQYY
                     IRVKEQEKFDTMTRLMDVAQPELAIVFGRTKRRVDELTRGLKIRGFRAEGIHGDLDQN
                     KRLRVLRDFKNGNLDVLVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KSGQSITFVAPNEMGYLQIIENLTKKRMKGLKPASVEESFQSKKQVALKKIERDFADE
                     TIRANFEKFGKDARKLAAEFTPEELAMYILSLTVQDPDSLPEVEIAREKPLPFKPSGN
                     GFGGKAKGGRGGRRGDDRRERDRRGNGRRDEFKKGSRGNDRFDKEKRYRKDNKKPRNT
                     LSEKQTGFVIRNKGDK"
BASE COUNT         2349 a         1615 c         1847 g         2463 t
ORIGIN      
        1 ttgtctaact tttggggggc agtacatatt caacacctga tactatgcgt tttattgtgg
       61 gaagattttc ctcattttct agtgaaattg aactttttct aacatctttt gatagatgac
      121 ttcttaatca attatcattt ccattgttgt ttcttggggt ttcatccctt gtctttgata
      181 aaagcggaga gcaccctcgt tatcattcca gacacttagg gttagattat tgcagccaat
      241 ttctttagca taatagcaga aaaatgacaa taaaatagcc cctagatatt ttgaaaggaa
      301 agcatagggg ctatatagtt taatcaaaat aaagcaaatc tttgctggtg cttgtaaaga
      361 ggttgaagcc atccttggta acaacaccgc agtcttcaat acgaacaccg actttaccag
      421 ggatatagat acctggttca acagagaagc acatgccttc ttcgatgacc atgtcgtttc
      481 cttccatgat agatgggaat tcatggacat ccataccgat accatgcccg agacggtggt
      541 tgaagtactc accataacca gctttttcga tgacctcacg ggcagcgcgg tccacttcat
      601 gagcagtcac acctggcttg ataaagtcaa gagcagcttg ttgggcttca agagtcaagt
      661 tgtaaatatc tttcttgaat tggtctggtt tgccgacagc gactgtacga gtcatatctg
      721 acgcatagcc attgaccaga acacccaggt caaagaggag aagagcatca ttttcaacct
      781 tattagctgc tggaatgccg tgtggattcg cagcattatc accagtcaag accatggtat
      841 caaagctcat ttcataacct tcacgtttca tggcaaagtc gatttgtgcg atgatatctg
      901 tctcagtctt atcaagagaa atattgtcaa aaccaacatg aacagccttg tcagcataaa
      961 gacctgcaac catcattttt tgcacttcat cagctgattt gatgaggcgc atgcgttgga
     1021 tacgaggagt gaggttgtca aactcagcag tctcaaaaac tgttttcaaa ccatggtatt
     1081 tggtcaagat gagattgtca aactcaacag cgacacgttt gaagtcaagt tgtggaagag
     1141 catgtttgat tttttgccat ggattttcag aatcgacata gcccactact gggaaggaaa
     1201 cggtgctact tgcacgttct acttcaagag ctgggacaaa gaggagaggt tcctgatctg
     1261 ctaggacaaa gaggaacatt tggcgttcat ggggatcact gtaaaaacca gtgaggtaat
     1321 tgattgtgac ggggtcagat acgacagcga cgtctagttt ttctgattca agatatgtta
     1381 ggatttgttg taatttagac atatgctacc ttctttctac ccctctattt tggcaaaaaa
     1441 tgagagaaaa tgcaagggag aagggtaaaa gaaaagacaa aaaactccga caagataacg
     1501 gagttctttg atatcatttt cctttgttta agaaaagtca gccttgcttt tgacgtcact
     1561 gtactcttca gcgatttctt ggctgagaat gtcctcatag gcagggagtt ggatgtcctg
     1621 accatatttc ttcttgaggg cagtagtaac taggcgataa gccagattgg cattacgaga
     1681 gaggataggc ccgtggaagt aggaaccaaa gacattctta taatgaaccc cttcaccgac
     1741 cttttcttcg ttgtttccat ttccatagac aacctgtccc agcggttttt ggtcatcaga
     1801 gaggaaggta cgaccttggt gattttcaaa tccatagtag gtttcatcga aatcttcatt
     1861 gtgaatcttg atgtcaccga taaaacggtt attggtctgg ttgagcgtgt agtgtcccat
     1921 gacccctagc ccttcgatac gttttcctga agcttcaaca taatattgac ccaatagttg
     1981 gaaaccaccg cagatagcca gaactacacc gtcgttttgg atgtagttgt caatgctctc
     2041 ttttttagca ggtaggtcgt ctgcaatgat actttgttca aagtcttgac caccaccgaa
     2101 aaaggcgatg tcgtagtgat tttcatcaaa gtcatcatgg agagaaacga tgtcaacggt
     2161 cacatgggct cccagttttt cagccacata cttgagcatg aggatgtttc cattgtcccc
     2221 gtaggtattc atgagatttc cgtagaggtg ggcaatgttg agctgatagg ggtaattgcc
     2281 atcttttgag gaaagtgaag tataaaccat tagttcatct cctttctaac aatctgacga
     2341 ctagccagca gttcacgaaa ttccagcatg gcagtataag ttgccagaat ataggcatgc
     2401 ttgcagtctt gattctcaat ggtcttgaga acttgctcca gattactcgt ttcagtgatt
     2461 ttctcagctg gatagccagt cactcggagg cgacgagcga tttcagaatg acgaacaccg
     2521 ccagcgttga tttcaggaat gtccatgtca gtgatttgtt caaagtctgc atcccagatc
     2581 cagctagtgt caattccatc tgcatagttg gcattaagga ggacagatag gctaaatgga
     2641 taaggtgcta gtttgatcat ttcgatagct tgggttgcac cgactggatt tttaatcaag
     2701 acaagggtac attccttgtc accgatatga aaggtttctt ggcgtccaaa gacagcacgg
     2761 ctcttgtcaa atccctgttt gatgagttgc gaatcggcac ctaggaaacg ggcgatggcc
     2821 acagcagcta gggcgttata gatattatag agcccgccga tttggatacc gtattcttgg
     2881 ccgtctatga caaagcgaga gcgattgttg gtcaactcaa ccagttttgt caaacgatag
     2941 tcgagatcag gacgtttaca tccacaacct tcacagatat aggcacccaa gtttgcatag
     3001 gtattatgct catatttgag gatgccttgg cagtcaggac agagaatccc ttcggtattg
     3061 tagtgagcca gttgggctgg tcccttttcc aagtcaaaac caaaatactc tatagggttt
     3121 ggaatagttg gcttgtagaa aagtggactg tctccgttaa ggagaacagt agcagttgga
     3181 actttccgaa tggcatccaa tatcatgtta taggtagtat agatttcacc gaaacggtcc
     3241 atctggtcac ggaagatatt agtaatgaca aaaagactag gctggatata gtcacagata
     3301 cgagatagac tggcttcgtc aatttcgagg acggcaatat ttttcccagt tttagaagat
     3361 ttggctgtta ggaaggttgt tgcaatccct gtaatcatgt tggcaccgct tgggttggtt
     3421 agaacttgac cataaacctc ttttaaaatg ccgacagtga gggcagttgt cagggttttt
     3481 ccatttgttc cagtgacaac gacaatctcg tagttcttag ctaggttttg taaaatatct
     3541 ttatcaaatt gaagggcgac tttccctggg agcgtacttc cacgtccaag acggcttaaa
     3601 acgaagtggg aagaacgccc agcaagaagg cccaaagtag tttttaagtt catgtttcta
     3661 ttatatcaca aaagtatatc acaaaagagg gaaaagacag atttggtgtt tcgtagaagt
     3721 aaaaacagcc caaagagggc tgtcagttaa gatgagattt caacttaaat cgcaaacaag
     3781 tcattcaagt tctcagccaa ggttgggtga gtgaagattt gttttgtgaa gtaagtgtaa
     3841 ggaatcttgt tgtccatagc aacagtaata atgttgatga tttcttgaga accttctgag
     3901 aagatgcttg caccaagaat ttcttttgtt tcagtattaa caacagcttt gaaagctccg
     3961 cgaaggtctc catttacgtg accacgaggc atggctgcaa cagggatttc tttcactgcg
     4021 tatggaagtt tcaaatcagc tgcttggctt tcagtcaaac caacttgtga aagtgcaggt
     4081 gtgatgaaca tagtatttgg cacattgaga cggtcctcaa gtgtgtagct gccatctcca
     4141 gcaaggtagc tgtaaacaac acggaagtca tcaagtgaaa tgtaagtaaa ttgaagacca
     4201 ccgttgacat ctccaactgc aaagacacca ggaacgtttg tttgacagtg tttatctact
     4261 ttaatagcgc cacgttcagt tagttcaata tctgtatttt caagttgaag tggttctaca
     4321 tttggtttgc gtccagttgc gtagagaagg gcgtcgaaac ggtaagtttc gtcttcagtt
     4381 acgacaagca cttggtcacc gtcgttttta atttcagtag tatggatatt ttgaagcaat
     4441 tcaataccgt cttcttccat gtattgttta gcaagagctg cgatggaagg ttctgcacga
     4501 ggtaggaatg tatccaaggc atctaggact gtaaccttgc ttcctagttt attgtaaagg
     4561 ccagcaaatt caagaccgat atttccgcca ccaaggactc caagtttttc aggcaattta
     4621 tccaagcttt ggatacctgt tgagtcaaag acgtttttgc ttgtagcaag tccagggatt
     4681 ggcaagacgt ttgaaacagc accagtgttg atgacgatag tttcagcagt caattcttgt
     4741 ttttcatcac cagcttggat ttcgatgact ttatttgaaa ggaagtgagc ttcagcatca
     4801 aagatatcga cgccagtacc agcaacagtc gtatagtttt taccgttgag gcgaccagtg
     4861 atcgtgtttt tggtagcaat gacttcttca aaagacaagt ctttctcagc agcaaccagc
     4921 aaagttttag ttgggataca accgatgttg atacaagttc caccgtacat agctttgcta
     4981 cgttcaacga gggcaacttt tttgccagct gaagccaatt taccagctag tgttttacca
     5041 gctttaccaa atccgataac gattaaatca taagttaaca tttagaattc ctcctattcg
     5101 aattacattc tagcacaaga aaaaaacttt tgcacaattc tgctacgaaa aagacagaaa
     5161 aatatgaaaa cgcttttcag gcaaaaaact agtaattttt tgacaagctt gataaactaa
     5221 aatcctatct atttttatat aagatagaat aaatgttcga aaataggagg ttttatgaaa
     5281 tcgaatcgtt atattattgc ctttgctggg gtcattttac acttaatgct gggttcgact
     5341 tatgcctgga gtgtttatcg taaccctatt attgaaaaaa cgggatggga tcaggcttct
     5401 gttgccttcg cctttagtct agcaatcttt tgtttgggct tatcggctgc atttatgggg
     5461 cgtttggtag aaaaatttgg tccgagagtc atggggagtc tatctgcttt tctatacgca
     5521 ggtggaaata tcttaacagg atttgcaata gaccgtcagg aactgtggtt gttgtatcta
     5581 gcttatggca ttttaggtgg gcttggtttg ggagcaggct atattacccc tgtgtcaacg
     5641 attataaaat ggtttcctga taaacgtggt ctcgcaacag gtttagcgat tatggggttt
     5701 ggttttgctt ctttattgac tagtcccata gcgcaacatc tcatcgcagg ggtagggctt
     5761 gtagaaactt tttatatttt aggtgcaagt tactttatta tcatgctcct agcttcacaa
     5821 ttcattaagc gtccaaatga gcaagagctt gcaattttat ctgcttcagg gaaagaaaaa
     5881 acagactctc tgacgcaagg gatgactgca aatcaggctc taaaaagcaa tcggttttat
     5941 atactttgga ttattttctt tatcaacata gcttgtggtt taggcttaat ttcagcggca
     6001 tcgccaatgg cacaggagat ggctggcttg tctacaagtc atgcagcagt aatggtgggt
     6061 gttttgggga ttttcaatgg atttggtcgc ttgctctggg cgagtttgtc tgactatatc
     6121 ggtcgccctc taacctttag tatattactg cttgtcaatc ttttcttttc tctctcactt
     6181 tggctcttta cagattccgt tttatttgta gttgctatgt ctattttgat gacttgctat
     6241 ggagctggtt tttctttgat tccagcttat ctcagtgata tttttggaac caaggaattg
     6301 gccgctctgc atggttatat tttaacagct tgggcaatgg ctggtttagc gggacctatt
     6361 ttattagcag agacttataa aatggctcat tcgtacacac aaaccttgtt cgtttttctc
     6421 attttataca gtatcgcctt ggctttgtct tattatctag gtcgttcaat caaaaaagaa
     6481 agtcaaaaac cgcttacatg attttctgta gaaatggggc tatcttttgc tctggtcttg
     6541 tgttatacta gataggttgc aaagaaaata gtacttttct tttgtggaaa agaagcaaca
     6601 cgattttata tctagttata tgaaaatacg tcataaaaag aaaagtataa actaagccct
     6661 atagggtggc ttcgcaccac ctttagaaag aagaataacg tgaaatttaa tgaattaaac
     6721 ttgtctgctg atttgctagc agaaattgaa aaagctggtt ttgtagaagc tagtcctatc
     6781 caagaacaaa ctattccttt ggcccttgaa ggcaaggatg ttatcggtca agctcagact
     6841 ggtacaggaa aaactgcagc ctttggcttg cctacccttg aaaaaatccg tacagaagaa
     6901 gcgactatcc aagccttggt catcgctcca actcgtgaac tagctgtcca aagtcaagaa
     6961 gaactcttcc gctttggtcg tagtaaggga gtcaaagtcc gttcagtata tggcggatca
     7021 agcattgaaa aacaaattaa ggctcttaaa tctggtgccc atatcgtggt gggaactcca
     7081 ggtcgcctct tggacttgat taaacgcaag gccttgaaat tacaaaacat tgaaaccctt
     7141 atccttgacg aagcggatga aatgcttaac atgggcttcc ttgaagacat cgaagccatt
     7201 atttcccgtg tacctgagaa ccgtcaaact ttgcttttct cagcaactat gccagatgcc
     7261 atcaaacgta tcggtgttca gtttatgaaa gcccctgaac atgtcaagat tgcggctaag
     7321 gaattgacaa cagaattggt tgaccagtac tatatccgtg ttaaggaaca agaaaaattt
     7381 gacaccatga ctcgtctcat ggatgtggca caaccagaac tcgctattgt atttggtcgt
     7441 accaaacgcc gtgtggatga attgactcgt ggtttgaaaa ttcgtggctt ccgtgcagaa
     7501 ggaattcatg gcgacctaga ccaaaacaaa cgtcttcgtg tccttcgtga ctttaaaaat
     7561 ggcaatcttg atgttttggt tgcgacagac gttgcagcgc gtggtttgga tatttcaggt
     7621 gtgacccatg tctacaacta cgatattcca caagatcctg agagttatgt tcaccgtatc
     7681 ggtcgtacag gtcgtgctgg taagtcaggt caatctatta cttttgttgc tccaaacgaa
     7741 atgggttacc ttcaaatcat tgaaaacttg actaagaaac gcatgaaagg tctcaaacct
     7801 gcaagtgtag aagaatcctt ccaatcaaaa aaacaggtag ctctcaagaa aatcgaacgt
     7861 gattttgcag atgaaaccat tcgtgccaac tttgagaaat ttggtaagga tgctcgcaaa
     7921 ttggctgctg agtttactcc agaagaattg gcaatgtata tcttgagtct gacagtccaa
     7981 gacccagata gccttccaga agtggagatt gcacgtgaaa aaccactacc gtttaaacca
     8041 tcaggtaatg gtttcggtgg taaagctaag ggaggtcgtg gaggccgtcg tggggacgac
     8101 cgtcgagagc gtgatcgccg tggcaatggt cgccgtgatg agttcaaaaa aggaagtcgt
     8161 ggcaacgatc gttttgataa ggaaaaacgt taccgtaagg ataataaaaa accacgcaat
     8221 actttaagcg aaaagcaaac aggctttgtt attcgtaaca aaggcgataa ataa
//
LOCUS       DAVLVP010000081         8170 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.081, whole genome shotgun sequence.
ACCESSION   DAVLVP010000081 DAVLVP010000000
VERSION     DAVLVP010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 8170)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8170)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8170
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.081"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            157..822
                     /locus_tag="VYN69_001662"
     CDS             157..822
                     /locus_tag="VYN69_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEV1533899.1"
                     /translation="MAIASGYLNSRLDKFVDWGPWTALVPFGLISVTNVGISMLSTRF
                     TGKLSKWGNYFGIVNTILSGAIDYILGNKAAIITYPVTFLIYTFAIKKWEASQEGRPN
                     QMSQKQVKLAAIIISIIAFLFAFVTNYIGYEGKMDLLAYVTTIAFALSLIANALNALK
                     LTTQWGFWLIYNFVQLTKAGIQGNFANIGKYIFYILNAIGALFVWNDEEVRYIEGNSN
                     KEY"
     gene            924..1337
                     /locus_tag="VYN69_001663"
     CDS             924..1337
                     /locus_tag="VYN69_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533900.1"
                     /translation="MKKLYRIHFIAIAVIDLLLFAFFITRLETSFECLLLSGLIFFLA
                     QGLLLFLLIVRLKHQFAEIYPQINKKIRFYYLGVLTIDFLFFVLLAFISSQRFSSLMS
                     IITACHSTFYYMTADYLRENYPDFYDKHISLWECL"
     gene            1356..1541
                     /locus_tag="VYN69_001664"
     CDS             1356..1541
                     /locus_tag="VYN69_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533901.1"
                     /translation="MKKIIFIKTIQLLVIDGIMLAFLTFKRGLTWDWILIYSGWLIFF
                     HPVLLTYLSNQLCDHFS"
     gene            complement(1808..2251)
                     /locus_tag="VYN69_001665"
     CDS             complement(1808..2251)
                     /locus_tag="VYN69_001665"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEV1533902.1"
                     /translation="MKIIIQRVKKAQVSIEGQIQGKINQGLLLLVGVGPEDQEEDLDY
                     AVRKLVNMRIFSDAEGKMNLSVKDIEGEILSISQFTLFADTKRGNRPAFTGAAKPDMA
                     SDFYDAFNQKLAQEVPVQTGIFGADMQVELVNNGPVTIILDTKKR"
     gene            complement(2281..4503)
                     /locus_tag="VYN69_001666"
     CDS             complement(2281..4503)
                     /locus_tag="VYN69_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019316080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEV1533903.1"
                     /translation="MPKEVNLTGEEVVALTKEYLTEEDVHFVHKALVYAVECHSGQYR
                     KSGEPYIIHPIQVAGILAKLKLDAVTVACGFLHDVVEDTDATLDDLEREFGPDVRVIV
                     DGVTKLGKVEYKSIEEQLAENHRKMLMAMSEDIRVILVKLSDRLHNMRTLKHLRKDKQ
                     ERISKETMEIYAPLAHRLGISSVKWELEDLSFRYLNPTEFYKITHMMKEKRREREALV
                     DEVVTKLEEYTTERHLKGKIYGRPKHIYSIFRKMQDKRKRFEEIYDLIAIRCILDTQS
                     DVYAMLGYVHEFWKPMPGRFKDYIANRKANGYQSIHTTVYGPKGPIEFQIRTKEMHEV
                     AEYGVAAHWAYKKGIKGQVNSKESAIGMNWIKEMMELQDQADDAKEFVDSVKENYLAE
                     EIYVFTPDGAVRSLPKDSGPIDFAYEIHTKVGEKATGAKVNGRMVPLTTKLKTGDQVE
                     IVANPNSFGPSRDWLNMVKTSKARNKIRQFFKNQDKELSVNKGREMLMAQFQENGYVA
                     NKFMDKRHMDQVLQKTSYKTEDSLFAAIGFGEIGAITVFNRLTEKERREEERAKAKAE
                     AEELVKGGEVKVENKETLKVKHEGGVVIEGASGLLVRIAKCCNPVPGDDIVGYITKGR
                     GVAIHRVDCMNLRAQENYEQRLLDVEWEDQYSSSNKEYMAHIDIYGLNRTGLLNDVLQ
                     VLSNTTKNISTVNAQPTKDMKFANIHVSFGIANLSTLTTVVDKIKSVPEVYSVKRTNG
                     "
     gene            5462..6091
                     /locus_tag="VYN69_001667"
     CDS             5462..6091
                     /locus_tag="VYN69_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000690864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1533904.1"
                     /translation="MKIHKTVNPVAYENTYYLEGEKYLIVVDPGSHWEAIRQTIEKIN
                     KPICAILLTHAHYDHIMSLDLVRETFGNPPVYIAESEASWLYTPVDNLSGLPRHDDMA
                     DVVTKPAEHTFVFHEEYQLEEFHFKVLPTPGHSIGGVSLVFPDAHLVLTGDALFRETI
                     GRTDLPTGSMEQLLHSIQTQLFTLPNYDVYPGHGSATTIAHEKAFNPFF"
     gene            complement(6214..8106)
                     /gene="pepO"
                     /locus_tag="VYN69_001668"
     CDS             complement(6214..8106)
                     /gene="pepO"
                     /locus_tag="VYN69_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase PepO"
                     /protein_id="HEV1533905.1"
                     /translation="MTRYQDDFYDAINGEWQQTAEIPADKSQTGGFVDLDQEIEDLML
                     ATTDKWLAGEEVPEDAILENFVKYHRLVRDFDKREADGITPVLPLLKEFQELETFADF
                     TAKLAEFELAGKPNFLPFGVSPDFMDARINVLWASAPSTILPDTTYYAEEHPQREELL
                     TLWKESSANLLKAYDFSDEEIEDLLEKRLELDRRVAAVVLSNEESSEYAKLYHPYSYE
                     DFKKFAPALPLDDFFKAVIGQLPDKVIVDEERFWQAAEQFYSEESWSLLKATLILSVV
                     NLSTSYLTEDIRVLSGAYSRALSGVPEAKDKVKAAYHLAQEPFKQALGLWYAREKFSP
                     EAKADVEKKVATMIDVYKERLLKNDWLTPETCKQAIVKLNVIKPYIGYPEELPARYKD
                     KVVNETASLFENALAFARVEIKHSWSKWNQPVDYKEWGMPAHMVNAYYNPQKNLIVFP
                     AAILQAPFYDLHQSSSANYGGIGAVIAHEISHAFDTNGASFDENGSLKDWWTESDYAA
                     FKEKTQKVIDQFDGQDSYGATINGKLTVSENVADLGGIAAALEAAKREADFSAEEFFY
                     NFGRIWRMKGRPEFMKLLASVDVHAPAKLRVNVQVPNFDDFFTTYDVKEGDGMWRSPE
                     ERVIIW"
BASE COUNT         2213 a         1883 c         1567 g         2507 t
ORIGIN      
        1 ttattttcag ggttttattt ctatcatttt attttacttt catttggtgg gagaatattt
       61 gaaaaaaaga aaggtagttt aacctatgaa aaaatcacta aaaatttttg ctacatctaa
      121 atagtttgac ctcttcgggg ttgctttggt cgttggattg cgattgcatc tggttacctc
      181 aactcacgcc tcgataaatt tgtagactgg ggaccatgga cagctcttgt accctttgga
      241 ttgatttccg taaccaacgt tgggatttcc atgttgtcca ctcgtttcac ggggaaatta
      301 agcaaatggg gaaattactt tggtattgtt aataccattt tgtccggtgc tattgattat
      361 atccttggaa ataaggcggc catcattacc tatcccgtca ccttcctcat ttataccttt
      421 gctataaaga agtgggaagc ttcgcaagaa ggcagaccca accaaatgag ccaaaaacag
      481 gtaaaattgg cggccatcat catttccatc atcgccttcc tctttgcctt tgtgaccaac
      541 tatatcggat atgagggcaa gatggatctc cttgcctacg taacaactat tgcctttgca
      601 ctgtccctca ttgccaatgc tttgaatgca ttgaaactaa caactcagtg gggcttttgg
      661 ttgatttaca atttcgttca gctgacaaaa gctggtattc aaggaaactt cgccaatatc
      721 ggtaaataca tcttttatat cctcaatgca atcggagctt tatttgtctg gaatgatgaa
      781 gaagttagat acatagaagg aaactcaaat aaagaatact agccaactta agagaatctc
      841 ccttctgcta tttttatcat tttagttgta actcccattc ttttggagta gaatgaagtt
      901 agataaaaga ggaaggtctt gtcatgaaaa aactctatag aatccatttt atagcaattg
      961 cagttattga tttgctacta tttgcatttt ttatcactag actcgagaca tcttttgagt
     1021 gcctcttgct ctctggttta attttcttcc ttgctcaagg acttctgcta tttctcttga
     1081 tcgtccgact caaacatcaa ttcgctgaga tttatcctca aatcaataaa aagattcgct
     1141 tctactattt aggggttctc accattgatt ttctattttt tgttctctta gccttcatta
     1201 gttctcagcg tttttcatct cttatgtcaa ttatcactgc ttgccattct actttttatt
     1261 atatgacagc tgactaccta agagaaaact atccagactt ttacgacaaa cacatctctt
     1321 tatgggagtg tctctaagga aaaggaggtt ttagcatgaa aaaaatcatc ttcatcaaaa
     1381 ccattcaact ccttgtcatt gatggaatca tgctggcatt tttgacattt aaaagggggc
     1441 ttacttggga ctggattttg atttatagcg gttggctcat tttctttcat cctgtgctat
     1501 tgacctatct ttcaaaccaa ctttgtgacc actttagtta actctattcc cagattagac
     1561 cgagattctg gcgttttgct ttacaaattc tcctatggga tagcctgatg attctctcct
     1621 tggtgtcttt aagtgatatt ccacttttcc ttcagggaac tctcctcatc ctaggacatc
     1681 tcatcccttc ctatcgcatc tgccaaagcc tgaaaagaga cttcccccaa gcatatcaag
     1741 aaccgatttc tttttggagt attttatgat agatgagaaa gaccaagccg actgggcttg
     1801 gtctttctta tctcttttta gtatctagga taatggtaac aggtccatta ttaaccagct
     1861 caacctgcat atctgctcca aagatgcctg tctgaacggg cacttcttgc gctaattttt
     1921 gattgaaagc atcatagaag tctgatgcca tatcaggttt agctgcccct gtaaaggctg
     1981 gacgattgcc tctcttagta tccgcaaaga gggtaaactg agaaatagag aggatttctc
     2041 cttcaatatc tttgacagac aggttcatct tgccttctgc gtctgaaaaa atccgcatat
     2101 tgaccagttt tctcacagca tagtccaaat cttcctcttg gtcctctggt ccaacaccaa
     2161 ccagcaataa aagtccctga ttgatttttc cctgaatctg gccttctata ctcacttggg
     2221 cttttttaac ccgttggata atgattttca taatagcctt tctagtaaga gctaggacaa
     2281 ctagccgttg gtccgtttga cagagtaaac ttctggcaca ctcttaattt tatcgacaac
     2341 cgtggtcagt gtagagaggt tggcaatacc gaaggacaca tggatattag caaacttcat
     2401 atccttggtt ggttgggcat tgaccgttga aatattcttg gttgtatttg aaagaacttg
     2461 cagtacatcg ttcaacagtc ctgtacggtt gagaccgtag atatcgatat gggccatata
     2521 ctccttattt gagctagagt actggtcttc ccattccaca tcaaggagac gttgctcgta
     2581 gttttcttgg gcacgcaggt tcatacagtc tacacggtga atagccacac cacgaccctt
     2641 ggtaatgtag ccaacaatat cgtcaccagg tacggggtta caacacttag caatccgcac
     2701 taggagacca gaagcacctt caataaccac tcccccctca tgcttgacct tgagagtttc
     2761 tttattttca accttgacct cgccaccttt gacaagctcc tctgcctcag ccttggcctt
     2821 ggcacgctct tcctcacggc gctccttttc agtcagacgg ttaaagacgg taatcgcacc
     2881 gatttcccca aaaccaatgg ccgcaaagag ggagtcttct gtcttgtaac tggtcttttg
     2941 cagaacttga tccatgtggc gcttgtccat aaatttattt gccacatagc cattttcttg
     3001 gaactgagcc atcagcatct cacgaccctt gttgacagac aattccttat cttggttttt
     3061 aaagaactgg cgaatcttat tgcgcgcctt gctagtcttg accatattga gccagtcacg
     3121 gctaggtcca aaggagttcg ggttggcgac aatttcaacc tgatcccctg tctttaactt
     3181 ggttgtcagt ggaaccatgc ggccattgac cttggcacca gttgcttttt caccgacctt
     3241 ggtatggatt tcgtaggcaa aatcaatcgg tcctgaatct ttgggaaggg aacggacagc
     3301 tccatctggg gtaaaaacgt aaatctcctc agccaaatag ttttccttaa cagagtccac
     3361 aaattcctta gcatcatcag cctggtcttg gagctccatc atctccttga tccagttcat
     3421 tccaatagct gattccttgc tgttaacttg cccctttata cctttcttat aagcccagtg
     3481 agccgcaacc ccgtactcag ccacctcgtg catttccttg gttcgaatct ggaattcaat
     3541 cggccctttt ggtccataaa cagtcgtatg gatagactga taaccattgg ccttgcggtt
     3601 ggcgatatag tctttgaagc gacctggcat cggtttccaa aattcatgca cgtaaccaag
     3661 catggcataa acatcacttt gggtatctaa aatacaacga atagcaatca gatcatagat
     3721 ttcctcaaac cgttttctct tgtcctgcat tttgcggaaa attgagtaaa tatgcttggg
     3781 acgaccataa atcttccctt tcaagtgacg ttctgtcgta tactcctcta attttgtgac
     3841 tacctcatcc accaaggcct cacgctccct gcgcttttcc ttcatcatat gggtaatctt
     3901 gtaaaactcc gttggattga gataacggaa agacaagtct tctaattccc atttgacact
     3961 ggaaatcccc aaacgatggg caagcggggc atagatttcc atggtttctt tggaaatacg
     4021 ctcctgcttg tcttttcgaa gatgtttcag ggtccgcata ttgtgcaagc ggtcagacag
     4081 tttgaccaaa ataacgcgga tgtcctcaga catggccatg agcatcttgc gatgattttc
     4141 cgctaattgc tcctcgatcg atttgtactc gaccttgcca agcttggtaa ctccgtcaac
     4201 aatcacccgc acatcaggac caaactctct ttccaaatcg tccaaggtcg catctgtatc
     4261 ttccaccaca tcatgcaaga atccacaagc tactgttaca gcatccagct ttagcttagc
     4321 taaaatacct gccacttgga tagggtgaat gatataaggc tcgcctgatt tgcgatattg
     4381 accactgtgg cattcaacag catagaccaa ggccttatgg acaaaatgaa catcctcttc
     4441 cgttaaatat tctttggtta aagcgacaac ttcttcgcct gttaaattca cttctttcgg
     4501 catctctact ctccaattct tcctaccatt ttatcacttt tttaagaata tgaaaactag
     4561 attggaacag aataagaaaa aaataattca aaattgcttg ataattctga attatcggcc
     4621 cgtaatatac tacgaagtta gattttaaac ttaggtgata gaaggagaga tagaagaacg
     4681 gaaaccatat tgtaacccaa agactttctg acttccccaa ttccattgaa gatacgaaag
     4741 ataaacggtg gaactcgtat cacatacact ggtaccttga ctggattttg gaattaatac
     4801 taaatgaaaa tcaaagagca aactaggaaa ctagccgcag gttactcaaa gcaccgcttt
     4861 gaggttgcag ataaagttga cgcggtttga agagattttt gaagagtata aaaatcctca
     4921 agatactttc ttctatcctt tagtttataa ggagaatacc tatgaaaaaa actgctattt
     4981 ctatctttgc tctcctaatg ttaggagttt gctgcctgtt cctattcagc cagcaaagct
     5041 ataaaaaaca gtcgttcaat actatgctaa cgaccagaac ctgcccagta ggataactta
     5101 tagtgaatat agcgacaaat gagaagccaa ctacggtagc actctaaaca tcacgtctat
     5161 caaacaagct aatgacggag tttatgcaac ctatgaaggg caattgacac ctttccaata
     5221 ttgataaatt gataaccagc ctgtcttcat ctagtcatgc tggtttttaa gttcatttta
     5281 aatccttacc tattctccct aactgtgcta tacttaattt atactcaatg aaaatcaaag
     5341 agcaaactag aaagctagcc gcaggctgtt caaagcactg ctttgaggtt gcagataaag
     5401 ttgacgcagt ttgaagagat tttcgaagag tattagtaca ttctttgaga ttggagctag
     5461 tatgaaaatc cataaaaccg tgaatcctgt tgcctatgaa aatacctatt atctagaagg
     5521 cgaaaagtac ctcatcgtcg tcgatcctgg tagtcattgg gaagccattc gtcagacaat
     5581 cgagaagatc aacaaaccga tctgtgctat tctcttgacc cacgcccatt atgaccatat
     5641 catgagtctg gacttggttc gcgagacgtt tggcaatcct cctgtctata tcgcagagag
     5701 cgaagccagc tggctctaca ctcctgtcga taatctctcc ggtctccctc gccacgatga
     5761 tatggcagat gtggtcacaa aacctgcaga acacaccttt gtctttcacg aagaatacca
     5821 actagaggaa tttcatttta aggttctgcc gaccccaggg cactctatcg gtggtgtttc
     5881 cctagtcttt cctgatgctc acctagtctt gacgggagat gctctattcc gcgaaactat
     5941 cggacggacc gaccttccga ctggtagcat ggagcaactc cttcatagta tccagaccca
     6001 actcttcacc ctaccaaact acgatgtcta tccaggacat ggttcagcta ctactatcgc
     6061 tcacgaaaag gccttcaatc ccttttttta gcaagatgat gacaatcgaa atttaagtaa
     6121 actatccagc aaatctttct attataaaag gcatcctatc aaggttttca cacgtgattg
     6181 gatgcctttt ttctgatgac tagatttttt gtattaccaa ataatcacgc gctcctctgg
     6241 tgaacgccac attccgtctc cttctttgac atcataggtt gtaaagaaat cgtcgaagtt
     6301 tggtacttgc acattgacac ggagtttggc tggtgcgtgc acatcgacgc tagccaaaag
     6361 tttcataaat tctggacgac ctttcatgcg ccagatgcga ccgaagttgt agaagaactc
     6421 ttctgctgag aagtctgctt ctctcttagc tgcttcaagc gctgctgcga ttcctcccaa
     6481 gtcagccacg ttttctgata cagtcaattt accgttaatg gttgctccat aagaatcctg
     6541 tccatcaaat tggtcaatga ctttttgtgt tttctccttg aaggcagcat agtcgctctc
     6601 tgtccaccaa tccttgaggc taccattttc gtcaaaggaa gccccgttag tatcaaaggc
     6661 gtgggaaatt tcatgggcaa tcactgcccc aataccaccg tagttagcag aagatgactg
     6721 atgcaagtca tagaaaggcg cctgtaaaat ggccgctgga aagacaatca ggttcttctg
     6781 aggattgtag taggcattga ccatatgagc aggcatgccc cattccttat agtctacagg
     6841 ctggttccac ttactccaac tgtgcttgat ttccacacgc gcaaaggcta gagcattctc
     6901 aaaaagactg gcagtttcat tcactacctt atccttgtaa cgtgcaggca attcttctgg
     6961 atagccaata taaggtttga tcacattgag cttcacgata gcctgtttac aggtttctgg
     7021 agtgagccag tcattcttaa gcagacgctc cttatagaca tcaatcatgg ttgccacttt
     7081 tttctccaca tccgccttgg cttctggaga gaacttctca cgggcgtacc aaagccccag
     7141 ggcttgcttg aaaggttctt gtgctagatg ataagctgct ttgaccttat cttttgcctc
     7201 tggaactcca gaaagggcac ggctgtaggc accagacaaa acacggatat cctctgttaa
     7261 atagctggtt gaaagattga caacactcaa aatcaaggtt gctttaagga gagaccagga
     7321 ttcctcactg tagaattgct ctgctgcttg ccagaaacgt tcctcgtcta caataacctt
     7381 gtctggtaat tgcccaataa ctgctttgaa gaagtcatcc aaaggtaggg caggcgcgaa
     7441 tttcttgaaa tcttcgtaag aatatggatg atagagttta gcatattctg aactttcttc
     7501 attagagagc accactgccg caactcggcg gtccaattca agtctttttt ctagcaagtc
     7561 ttcaatttct tcatcagaga aatcataagc cttgaggaga tttgcgctgc tttctttcca
     7621 aagagtcaag agctcttcgc gctgaggatg ttcttctgca tagtaggtcg tatctggcaa
     7681 gattgtgctt ggagcgctag cccatagaac attgattcta gcatccataa agtctggcga
     7741 tacaccaaaa ggaaggaagt ttggttttcc tgcaagctca aactctgcta gtttagctgt
     7801 aaaatccgca aaagtttcca attcttggaa ttctttaagg agtggtaaga caggtgtgat
     7861 accgtcagct tctctcttgt caaaatcacg aactaggcgg tggtatttga caaagttttc
     7921 caagatagca tcctcaggca cttcttcacc tgctaaccac ttgtctgttg tcgccaacat
     7981 caggtcttca atttcctggt ctaaatcaac aaaacctcct gtttgagact tatctgctgg
     8041 gatttcagct gtctgttgcc attctccatt gatagcatca taaaaatcat cttgataacg
     8101 tgtcatcttg ttctcgcttt catttgtatt tgcatttatc ttaacaaaaa tcactaggga
     8161 atgcaattaa
//
LOCUS       DAVLVP010000082         7449 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.082, whole genome shotgun sequence.
ACCESSION   DAVLVP010000082 DAVLVP010000000
VERSION     DAVLVP010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7449)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7449)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7449
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.082"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            120..275
                     /locus_tag="VYN69_001669"
                     /pseudo
     CDS             120..275
                     /locus_tag="VYN69_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
     gene            475..3750
                     /gene="rexB"
                     /locus_tag="VYN69_001670"
     CDS             475..3750
                     /gene="rexB"
                     /locus_tag="VYN69_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002206154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent nuclease subunit B"
                     /protein_id="HEV1533906.1"
                     /translation="MKLLYTDIRTSLTEILTREAEELVAAGKRVFYIAPNSLSFEKER
                     AVLECLSQQASFSITVTRFAQMARYLVLNDLPVKTTLDDIGLGLAFYKCLAELDPKDL
                     RVYGAIKQDPQLIQQLIELYHEMTKSQMSFLDLENLTDEDKRTDLLLIFEKVTAYLNQ
                     GQLAQGSQLSHLIEAIENDKVSSDFNQITLVIDGFTRFSAEEERVVDLLHGKGVEIVI
                     GAYASKKAYTSPFSEGNLYQASVKFLHHLASKYQTPAQDCSQTHEKMDSFDKASRLLE
                     SSYDFSELALDVDEKDRENLQIWSCLTQKEELELVARSIRQKLHENSDLSYKHFRILL
                     GDVASYQLSLKTIFDQYQIPFYLGRSEAMAHHPLTQFVESILALKRYRFRQEDLINLL
                     RTDLYTDLSQSDIDAFEQYIRYLGINGLPAFQQIFTKSHHGKFNLERLNVLRLRILAP
                     LETLFASRKQKAENLLQKWSVFLKEGAVTKQLQDLTTTLEAVEQERQAEVWKAFCHVL
                     EQFATVFAGSQVSLEDFLALLHSGMSLSQYRTIPATVDTVLVQSYDLIAPLTADFVYA
                     IGLTQDNLPKISQNTSLLTDEERQNLNQATEEGVQLLIASSENLKKNRYTMLSLVNSA
                     RKQLFLSAPSLFNESESKESAYLQELIHFGFRRREKRMNHKGLSKEDMGSYHSLLSSL
                     VAYHQQGEMSDTEQDLTFVKVLSRVIGKKLDLQGLENPAIPTSPSSKTLAKDTLQALY
                     PAKQEFYLSTSGLTEFYRNEYSYFLRYVLGLQEELRLRPDARSHGNFLHRIFERALQL
                     PNEDSFDQRLEQAIQETSQEREFEAIYQESLEAQFTKEVLLDVARTTGHILRHNPAIE
                     TIKEEANFGGKDQAFIQLDNGRSVFVRGKVDRIDRLKANGAIGVVDYKSSLTQFQFPH
                     FFNGLNSQLPTYLAALKREGEQNFFGAMYLEMAEPVQSLMAVKSLAGAVVEASKSMKY
                     QGLFLEKESSYLGEFYNKNKANQLTDEEFQLLLDYNAYLYKKAAEKILAGRFAINPYT
                     ENGRSIAPYVQQHQAITGFEANYHLGQARFLEKLDLADGKRLVGEKLKQAWFEKIREE
                     LNR"
     gene            3747..7397
                     /gene="addA"
                     /locus_tag="VYN69_001671"
     CDS             3747..7397
                     /gene="addA"
                     /locus_tag="VYN69_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEV1533907.1"
                     /translation="MKLIPFLSEEEIQKLQEAEANSSKEQKKTAEQIEAIYTSGQNIL
                     VSASAGSGKTFVMVERILDQLARGVEISQLFISTFTVKAATELKERLEKKISKKIQET
                     DDVDLKQHLGRQLADLPNAAIGTMDSFTQKFLGKHGYLLDIAPNFRILQNQSEQLLLE
                     NEVFHEVFEAHYQGKQKESFSHLLKNFAGRGKDERGLRQQVYKIYDFLQSTSNPQKWL
                     SESFLKGFEKADFTSEKEKLTEQIKQALWDLESFFRYHLDNDAKEFAKAAYLENVQLI
                     LDEIGSLNQEFDSQAYQAVLARVVAISKEKNGRALTNASRKADLKPLADAYNEERKTQ
                     FTKLGQLSDQIAILDYQERYHGDTWKLAKTFQSFMSDFVEAYRQRKRQENAFEFADIS
                     HYTIEILENFPQVRESYQERFHEVMVDEYQDINHIQERMLELLSNGHNRFMVGDIKQS
                     IYRFRQADPQIFNEKFQRYAQNPQEGKLILLKENFRSSSEVLSATNDVFERLMDQEVG
                     EINYDNMHQLVFANTKLTPNPDNKAEFLLYDKDDTGEEEESQTETKLTGEMRLVIKEI
                     LKLHQEKGVAFKEIALLTSSRNRNDQILLALSEYGIPVKTDGEQNNYLQSLEVQVMLD
                     TLRVIHNPLQDYALVALMKSPMFGFDEDELARLSLQKAEDKAHENLYEKLVNAQKKAS
                     SQKGLIHTALAEKLKQFMDILASWRLYAKPHSLYDLIWKIYNDRFYYDYVGALPNGPA
                     RQANLYALALRADQFEKSNFKGLSRFIRMIDQVLEAQHDLASVAVAPPKDAVELMTIH
                     KSKGLEFPYVFILNMDQDFNKQDSMSEVILSRQNGLGVKYIAKMETGAVEDHYPKTIK
                     LSIPSLTYRQNEEELQLASYSEQMRLLYVAMTRAEKKLYLVGKGSREKLESKEYPAAK
                     NGKLNSNTRLQARNFQDWLWAISKVFTKDKLNFSYRFIGEDQLTREAIGELETKSPLQ
                     DSSQADNRQSDTIKEALEMLKEVEVYNTLHRAAIELPSVQTPSQIKKFYEPVMDMEGV
                     EIAGQGQSVGKKISFDLPDFSTKEKVTGAEIGSATHELMQRIDLSQQLTLASLTETLK
                     QVQTSQAVRDKINLDKILAFFDTALGQEIFANTDHLYREQPFSMLKRDQKSQEDFVVR
                     GILDGYLLYENKIVLFDYKTDRYDEPSQLVDRYRGQLALYEEALSRAYSIENIEKYLI
                     LLGKDEVQVVKV"
BASE COUNT         2271 a         1568 c         1583 g         2027 t
ORIGIN      
        1 gattgaaata agatacgaac agcttgatta gggaaatcaa attaatttct agaaatattt
       61 tagcatccac gatgtactat tatagtttca atttactata gttcaatcat tctatcaaga
      121 atattgaata tataaaggca cgtaatatta aaacttcttc tgatatcgaa ttagagtccg
      181 atgttgatgg cgataaatca gacaatctac ccgtcgacat taagatactg ggacaacaca
      241 ttgaagtgta ttccgtacct aagaaacaat cataaaactc aaagtcttgt taccgaggct
      301 tttttattat gtgattttca gtagccagac ttattctatc taatacaagt aaagctaagt
      361 atttaagatt tagcagatga tgaagaaaag aaaggatggc ctaaatattt taacagattt
      421 acgaggctaa tcatggtata atatagggtg atgaaaaatt ccaacgaggc tgagatgaaa
      481 ttactttata ctgatattcg aacttctttg acagaaattc taacaagaga ggcagaagag
      541 ctagttgcag ctggcaagcg ggtcttctac attgccccca actctctttc ttttgaaaag
      601 gaacgcgccg tgctggaatg cttgtcccag caggcttctt tttctattac cgtcacgcgc
      661 tttgctcaaa tggctcgtta tctggtcctg aatgatttac cagttaaaac tactcttgat
      721 gatatcggtc ttgggttggc cttttacaaa tgccttgccg aactcgatcc caaggacttg
      781 cgtgtttatg gcgctattaa gcaggatcct caattgatcc agcagttaat tgagctttac
      841 catgagatga ccaaatctca gatgagtttt ttggacttgg agaatttaac agatgaggat
      901 aagaggacgg atttactctt gatttttgag aaagtaacag cctatcttaa tcaaggtcag
      961 ttagcccagg gaagtcagtt gtcccatttg attgaggcta ttgagaatga caaggtaagt
     1021 agtgatttta atcaaatcac cttggtcatt gacggcttta ctcgtttttc tgctgaggaa
     1081 gagcgggttg tggacttact tcacggcaaa ggtgttgaga ttgttatcgg ggcttatgct
     1141 agtaagaaag cctataccag tccttttagc gagggcaatc tctaccaagc cagcgtaaaa
     1201 tttctccatc atctggcttc taaataccaa acgcctgctc aggactgttc tcaaactcat
     1261 gagaagatgg atagttttga caaggcctct cgtttgttgg agtcttctta tgacttttca
     1321 gaactcgctt tggatgtcga tgagaaagac cgtgaaaatt tacaaatctg gtcttgtttg
     1381 acgcaaaagg aggagttgga gctagtagcc cgtagcattc gtcagaaatt acatgagaac
     1441 tcagacctga gctacaagca ttttcgtatt ctcttggggg atgtagcttc ttaccagtta
     1501 tctctcaaaa ccatttttga ccagtatcag attccttttt atcttggtag aagcgaagcc
     1561 atggctcatc atcccttgac tcagtttgtc gagtctattt tagctttaaa acgttaccgt
     1621 tttcgtcagg aggatttgat taatcttctt agaactgatt tgtatactga cctcagtcag
     1681 tctgatattg atgcttttga gcaatatatc cgctatcttg gtatcaatgg cttgccagcc
     1741 tttcagcaaa tcttcaccaa atcccaccat ggaaaattta atcttgagcg tttgaatgtc
     1801 ctccgcctga gaattttagc acctcttgaa accctctttg ccagccgaaa acaaaaggct
     1861 gaaaacctcc tacaaaaatg gagtgtcttt ctaaaagaag gagctgtgac caagcagtta
     1921 caagatttga caaccacttt ggaagctgta gaacaggaaa gacaagccga agtttggaag
     1981 gctttctgcc atgttttaga acaatttgcg actgtttttg ctggttcaca ggttagtctg
     2041 gaagacttcc tagccttgct ccattctgga atgagtttgt cccaataccg taccattcca
     2101 gcaacagtgg acactgttct ggtgcagagt tacgatttga ttgcaccatt gactgctgac
     2161 tttgtctatg ctattggact aactcaggac aatttaccaa aaatttctca aaacaccagt
     2221 cttctgacag atgaagaaag gcaaaatcta aaccaagcga ccgaagaagg cgttcaatta
     2281 ctgattgcca gcagtgaaaa tctcaagaaa aatcgctaca ctatgctttc cttggtcaat
     2341 tctgctcgta agcagttgtt cttgtcggct ccaagccttt ttaacgaaag tgaaagtaag
     2401 gaatctgcct atcttcaaga gttgatccat tttggattta ggcggagaga gaagaggatg
     2461 aatcacaaag gactgtctaa ggaggatatg gggtcctatc acagtctttt gtctagtctg
     2521 gttgcctatc accagcaggg tgagatgagc gatactgagc aagatttgac ttttgtcaag
     2581 gttctgtcgc gtgtcatagg taaaaaacta gatctgcaag gtctggaaaa tccagctatc
     2641 ccaaccagtc caagcagcaa gaccttagcc aaggacacct tgcaagctct ctatcctgcc
     2701 aaacaggagt tttacctgtc tacgtcgggt ttgacagagt tttatcgcaa tgaatacagt
     2761 tatttcctac gctacgtttt aggcttgcag gaggaattac gtttgcgtcc tgatgcccgt
     2821 agtcacggga atttcttgca tcgtatcttt gaacgcgcct tacagttgcc taatgaagat
     2881 tcctttgacc aacgtctaga acaagctatt caagaaacca gtcaagaacg cgaatttgaa
     2941 gctatttatc aagaaagttt ggaagcccag tttaccaagg aagttttgct tgatgttgca
     3001 cggacgactg gacatattct ccgacacaat ccagccatcg aaaccatcaa agaagaagca
     3061 aattttggtg gaaaagacca agcctttatt caattagaca atggacgcag tgtctttgta
     3121 cgaggcaagg tggaccggat tgaccgtttg aaagctaatg gagcgatagg agtagtagac
     3181 tacaaatcca gtctgactca gttccagttt cctcatttct ttaatgggct caattctcag
     3241 ttaccaacct atcttgctgc cctaaaaaga gaaggggagc agaacttttt cggcgccatg
     3301 tacttggaaa tggctgaacc tgtccaatct ctgatggcgg taaaaagtct ggcaggagca
     3361 gtggtagaag ccagcaaatc tatgaaatac caagggctct tcttggaaaa agaaagcagt
     3421 tatttaggcg aattttataa caaaaacaag gctaatcaac tgacagatga ggaatttcag
     3481 ctcctactgg actacaatgc ctatctttac aagaaagctg ctgagaagat tttagcaggc
     3541 cggttcgcca tcaatcctta tactgaaaat ggcagaagca ttgccccata cgtccagcaa
     3601 catcaggcta ttacaggctt tgaagccaat taccatctgg gccaagcccg tttcctagaa
     3661 aagttggacc tagctgatgg caagcgtctg gtcggagaaa aactcaagca agcttggttt
     3721 gaaaaaataa gagaggagtt aaatcgatga agcttattcc ctttttaagt gaggaggaga
     3781 ttcaaaaact gcaagaagca gaagcaaatt cgagcaagga acagaagaaa actgccgaac
     3841 aaatcgaagc tatctacact tctggtcaga atatcctggt ctcagcatcg gctggttctg
     3901 gaaagacctt tgtcatggta gagcgcattc tggaccaatt ggcgcgtggt gtcgaaattt
     3961 ctcaactctt tatctcaacc tttaccgtca aggctgcaac tgaacttaaa gaacgtttag
     4021 agaaaaaaat cagcaagaaa atccaagaaa cagatgatgt cgatctcaaa caacacttgg
     4081 gtcgccagtt ggcagaccta cccaacgctg ccatcggaac catggactct ttcacacaaa
     4141 aattccttgg caaacatggt tatctgcttg atattgcacc taatttccgt attttacaaa
     4201 accaaagcga gcaacttctt ctcgaaaacg aagtctttca tgaggtcttt gaagcgcatt
     4261 accaaggtaa acagaaagag agctttagtc atttgctgaa aaactttgct gggcgtggca
     4321 aggacgaacg gggtctgcgg cagcaagtct ataaaatcta tgacttcctc caatccacca
     4381 gtaatcctca aaagtggctg agtgaatctt tcctcaaagg ctttgagaaa gctgatttta
     4441 ccagtgaaaa agaaaaactg actgagcaaa tcaaacaagc cctctgggat ttggaaagct
     4501 ttttccgtta ccatctggat aacgatgcca aggagtttgc aaaggctgcc tatttagaaa
     4561 atgttcagtt aattctggat gaaattggct ccctaaatca ggagttcgat agtcaggctt
     4621 atcaggcagt acttgcgcgt gttgtcgcca tctctaagga gaaaaacggt cgagctctga
     4681 ctaatgccag ccgtaaggct gatttgaagc ccctggctga tgcctacaac gaagagagaa
     4741 agacccagtt tactaaacta ggacaattat cagaccagat agcgattctc gactatcaag
     4801 aacgttatca tggagacact tggaaactag ctaaaacctt ccaatctttc atgagcgatt
     4861 ttgtagaggc ttatcgtcag agaaaacgac aggaaaatgc ctttgaattc gctgatatca
     4921 gccattacac cattgagatt ttagaaaatt tcccacaagt tcgtgagtct tatcaggagc
     4981 gcttccatga agtcatggtc gatgagtatc aggatatcaa ccatattcaa gaacggatgc
     5041 tggaattgtt gtctaatggc cacaatcgct ttatggtggg agatatcaag caatccatct
     5101 atcgtttccg tcaggcagac ccgcagattt tcaatgagaa attccaacgc tatgcgcaaa
     5161 atcctcaaga aggcaagctc attctcctca aggaaaattt ccgtagtagt tcagaagtgc
     5221 tgtcagcaac caatgatgtc tttgaacgtc tcatggacca agaggtcggc gaaatcaact
     5281 atgataacat gcaccagctt gtttttgcca ataccaaact gactcccaat ccagacaaca
     5341 aggccgaatt tctcctctac gacaaggacg atacaggtga ggaagaagag agtcaaacag
     5401 aaacgaaact aacaggcgaa atgcgcttag ttatcaagga gatccttaaa cttcatcagg
     5461 aaaaaggtgt tgcctttaag gaaattgccc ttctgacctc tagtcgtaat cgtaatgacc
     5521 aaattctact tgccctgtct gagtacggga ttcctgtcaa aactgacgga gagcaaaaca
     5581 attatctcca atccctagaa gtgcaagtca tgctagacac ccttcgtgtc attcacaatc
     5641 ccctgcaaga ctacgccttg gttgccctta tgaagtctcc aatgtttggt tttgatgagg
     5701 atgagctagc acgtttgtcc cttcagaaag cagaggataa agcccacgaa aatctctatg
     5761 agaaactagt caatgcacaa aaaaaggcaa gtagtcaaaa aggcttgatt cacacagctc
     5821 tagctgaaaa actaaagcaa ttcatggata tcctagcttc ttggcgcttg tatgccaaac
     5881 cccactctct ctatgacttg atttggaaga tttacaacga ccgtttttat tatgactatg
     5941 ttggggcttt gccgaatggt cctgctaggc aggccaatct ctatgcccta gcactgcgtg
     6001 ctgatcaatt tgaaaagagc aatttcaaag gtttgtcgcg ttttattcgt atgattgacc
     6061 aagtcttaga agcccagcac gatttggcaa gcgtggccgt cgcaccgcca aaagatgcag
     6121 tagagctcat gaccatccac aagagtaaag ggctggagtt tccttacgtc tttatcctca
     6181 atatggatca agatttcaac aaacaagact ctatgtcaga agtcattctc agtcgtcaga
     6241 atggtcttgg tgtcaaatat attgccaaga tggagacagg ggcagtagaa gaccactatc
     6301 ctaaaaccat caaactctcc attcctagtc tgacctatag gcagaacgaa gaggaattac
     6361 agctagcaag ctattctgag cagatgcgtt tgctgtatgt tgctatgacg cgggctgaga
     6421 aaaagctcta tcttgtcggc aagggttctc gtgaaaagct ggaatccaag gaatacccag
     6481 cagccaaaaa tgggaaacta aatagcaata ctagactgca agcacggaat ttccaagatt
     6541 ggctttgggc tatcagtaaa gtgtttacta aggacaagct caactttagt tatcgtttta
     6601 ttggcgaaga tcagttgacc agagaagcta tcggagagtt ggaaaccaag agtcctctcc
     6661 aagatagctc ccaagcagac aatcgtcagt cagataccat caaagaagct ctggaaatgc
     6721 tgaaggaggt ggaagtttat aatactcttc accgcgcagc tattgaactt cctagtgttc
     6781 aaaccccaag tcaaatcaag aaattctacg aaccagttat ggatatggaa ggtgtcgaga
     6841 ttgctggtca aggtcagtca gtaggcaaga aaatcagctt cgatttgcca gatttttcaa
     6901 ccaaagaaaa ggtaactgga gctgagattg gtagtgctac tcacgaactc atgcagagaa
     6961 ttgacctcag ccagcaacta acccttgcta gcctaacaga aacactcaaa caagttcaaa
     7021 ctagccaagc tgtcagagac aagatcaatc ttgataaaat tcttgctttc tttgacacag
     7081 cactcggtca ggaaattttt gctaataccg accatctcta tcgcgagcaa cctttctcca
     7141 tgctcaaacg agaccaaaag agtcaggaag actttgttgt ccgtggtatc cttgatggct
     7201 atctgcttta cgaaaacaaa attgttttgt tcgactacaa gacagaccgc tatgatgaac
     7261 caagtcaact cgtagaccgc tatcgtggtc agttagctct atacgaagag gctttatcac
     7321 gagcctattc gattgaaaat attgaaaaat acttgatttt actcggtaaa gacgaggttc
     7381 aagttgtaaa agtataactt atactcttcg aaaatctctt caaaccacgt cagcttcgcc
     7441 ttgctgtat
//
LOCUS       DAVLVP010000083         7222 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.083, whole genome shotgun sequence.
ACCESSION   DAVLVP010000083 DAVLVP010000000
VERSION     DAVLVP010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.083"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            361..741
                     /locus_tag="VYN69_001672"
     CDS             361..741
                     /locus_tag="VYN69_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SP_0115 family bacteriocin-like peptide"
                     /protein_id="HEV1533908.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGGVGRNWWNSRGSFATVLDVDL
                     AIYSGGATIYSADAIKKAISANRGAITRTLRSLIIKHVGSAAGHLVNTALNVALTVTG
                     FSLGGAIAYGVDWADGSLDGYIFA"
     gene            complement(1142..2005)
                     /locus_tag="VYN69_001673"
     CDS             complement(1142..2005)
                     /locus_tag="VYN69_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001268159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1533909.1"
                     /translation="MRYDFGKVYKEIRESKGLTQEEVCGGVLSRTSLSKIESGKTTPK
                     YENMEFLLRQINMSFEEFEYICQLYQPSQRTEIMQTYLNMRSIIGTSDLVNLFQKCQD
                     YLKTHHDLPIEEIRDMLEVVIYIRQHGAGELSDHAEQVVKKLWRRIEKQDTWYESDLK
                     ILNTILFSFPIEYLHLITGKILQRLEVYKNYQHLYDLRIAILLNLSTLYLYNQDKNMC
                     KQICYTLLEDAKNKKSYDRLAICYVRIGICTDNAKLIQKGFSLLELTEETSMLSHLKK
                     EVETHYQPKKL"
     gene            2471..2809
                     /locus_tag="VYN69_001674"
     CDS             2471..2809
                     /locus_tag="VYN69_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C39"
                     /protein_id="HEV1533910.1"
                     /translation="MLKKYPCTMQHDQSDCAAAVVSTVLLSYKKELSIMKIREIIGTD
                     MYGTTVSGIVLGLNKLNFTVKAVRVALEDLTPKLTFPAILQVKNDLGQNHFVVLHSIK
                     EKINGTRITK"
     gene            2787..3281
                     /locus_tag="VYN69_001675"
     CDS             2787..3281
                     /locus_tag="VYN69_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533911.1"
                     /translation="MELVLPNNYVALEQEEMMYLDGGAYLSKSACQGICAALAMSSGT
                     FIALTGAAVLTKKLINYIKVGGLGGWLIGAAAGVLALIFLIKICVDTTIKFLFIRLFD
                     TSTLMKRRIFFLFALDVIAYLFLGYLLVIQKAGYLFSVLLLFSNFSVPFIKEKEFELF
                     KNSK"
     gene            4418..4921
                     /locus_tag="VYN69_001676"
     CDS             4418..4921
                     /locus_tag="VYN69_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533912.1"
                     /translation="MKLLDCILDYQERFNGKTCQVSTNYKYLEIFKVNFCLTDLHHLF
                     GLHKITRDYASQTKPAIQTGVFILEDFRNILLYNDVIERISLYEFISDIFYSKLTSCC
                     IVAKDLSKNTMKLDVIFFEDKNKRSAVLGLRRDKSGVFKPVTLHFTSAKKYAKVRKTD
                     VKEMKWL"
     gene            5206..7113
                     /gene="pspA"
                     /locus_tag="VYN69_001677"
     CDS             5206..7113
                     /gene="pspA"
                     /locus_tag="VYN69_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001035388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal surface protein A"
                     /protein_id="HEV1533913.1"
                     /translation="MNKKKMILTSLASVAILGAGFVTSSPTFVRAEEAPVASQSKAEK
                     DYDAAKKDAKNAKKAVEDAQKALDDAKAAQKKYDEDQKKTEKKAAAVKKIDEEHQAAN
                     LKSQQALVEFLAAQREGNPKKKKAAQAKLEEAEKAEKEKKKEFDKAQAVVVPEATELA
                     ETKKKADEAKVKEPELTKKLEEAKAKSEEAEKKATEAKQKVDAEHAKEVVPQAKIAEL
                     ENEVQKLEKDLKEIDESDSEDYVKEGLRAPLQSELDAKQAKLSKLEELSDKIDELDAE
                     IAKLEKNVEDFKNSNGEQAEQYRAAAEEDLAAKQAELEKTEADLKKAVNEPEKPAPAP
                     ETPAPEAPAEQPKPAPAPQPAPAPKPEKPAEQPKAEKTDDQQAEEDYARRSEEEYNRL
                     TQQQPPKAEKPAPAPKPEQPAPAPKIGWKQENGMWYFYNTDGSMATGWLQNNGSWYYL
                     NANGSMVTGWLQNNGSWYYLNANGSMATGWLQNNGSWYYLNANGAMATGWLQNNDSWY
                     YLNASGAMATGWAKVNGSWYYLNANGAMATGWLQYNGSWYYLNANGAMATGWVKVNGS
                     WYYLNANGSMATGWVKDGDTWYYLEASGAMKASQWFKVSDKWYYVNGLGALAVNTTVD
                     GYKVNANGEWV"
BASE COUNT         2455 a         1057 c         1482 g         2228 t
ORIGIN      
        1 ggagcttgag ttgttagtgg aggataaaat gttaagagta atagtagtta ttcaatcttt
       61 agtaataata ctgcttgctg ttgctttatt attatctcgg aaaaactttg attcgttaaa
      121 tgttgttctt ataatgatga tcgtatcaca attgttgtta ttagtatatt tgataaaatc
      181 agggaatcag tgattagctg ttatttgaat taagtaaact gttaagcttc aagcagtaac
      241 tggttttaaa tatttaaaat aagataaagg aaggaagtct aatttaggta actaaacaaa
      301 gcctgcgatc acttgttagg aaaccatcct aaccatgctt aataaaaaag gagaaaataa
      361 atggaactcg tattaccaaa taattatgtt gctcttgagc aagaagagat gatgtatctt
      421 gatgggggtg gtgttggtcg taactggtgg aatagtagag gtagttttgc aacagttctg
      481 gatgtagatt tggccatcta tagtggtggt gcaacaattt attctgctga tgcgataaaa
      541 aaagctatct cagctaatag aggggctatt acgagaacat tacgtagttt aataattaaa
      601 catgtaggta gtgcagctgg ccatttagtc aatactgcac taaacgttgc actaactgtt
      661 actggatttt cactaggtgg agcaatcgca tatggggttg attgggctga cggtagctta
      721 gatggttata tttttgctta aaatgaaaca tttttttgca ggaattggtg aaataaactt
      781 tgtaagttat ttattgtata tatgtgtagg aatagcccct ttttttcacg tatatataat
      841 aggttctgaa atgaattttg tgaaaatagt gttatctatt ttgggaatta tatttgtttg
      901 tattttaact attgctcgaa tttataggac ttttttctat aaagaatagt ttggacttga
      961 aatgtattta aaaacaagag gttggtagat tatcagcctc tttcttgtcg ttgaaaaagt
     1021 gagatatatt gacttttgaa aaaaatgtca taattgagtt gggataaacg taaaagtcat
     1081 actttctcgt taaatcgttc ggcgattcta tgaaaaggtc gaggagctcc ctcgactttt
     1141 tttataattt ctttggttga taatgggtct ctacttcttt tttgagatga gacagcatag
     1201 aagtttcctc ggtcagctct aggagtgaga aacctttttg gataagttta gcattatctg
     1261 tacaaatccc aatacggaca tagcagatag caagcctatc gtagcttttc ttattcttgg
     1321 cgtcctccag taaagtgtag cagatttgct tacacatgtt tttatcttga ttgtatagat
     1381 agagtgtgga aaggttgagc aggattgcta ttcgcaagtc atataaatgt tgatagtttt
     1441 tatagacttc taggcgttgc aatatttttc cagtgatgag atggaggtat tcaatgggaa
     1501 aactgaaaag aatggtattg aggattttta gatcactctc ataccatgta tcttgttttt
     1561 caattcttct ccaaagtttt ttgacaacct gttctgcgtg atctgatagt tcccctgccc
     1621 cgtgttgacg gatataaatg acaacttcaa gcatatctct gatttcttct atagggagat
     1681 cgtggtgggt cttgaggtag tcttggcatt tttgaaataa attgacaaga tcgctggtcc
     1741 cgataatgga acgcatattg agataggttt gcataatctc tgtgcgttga cttggttgat
     1801 aaagttgaca gatatattca aactcttcaa aactcatatt gatttgacgg agaagaaatt
     1861 ccatattttc atattttggg gttgtcttgc cactttcgat ttttgataag ctggttcttg
     1921 agagaacacc cccacagacc tcttcttggg tcagcccctt tgactcacgt atttctttat
     1981 agacctttcc gaaatcataa cgcatgattt tctcctttct gtgaaaaaag ttaacaaata
     2041 ataaaatctt gttaaaaata ttgtatcata gcaatgtaaa aagtaaaaaa gaaagtcgaa
     2101 ataaatttgt gaaaaaagtt aacgtaattt taaaatgagc ttaataagag gtatgatgta
     2161 ggtagaaaat aaagatagga gaaaaagtat gaataaaaaa atattttgta ttctagtatg
     2221 tattatatca ttgattagtc tggccataat ctttccatgg ggctggtcga tataatgtaa
     2281 gggagcttga gttgttaaag gaggcttagt ccttggacaa aaatctgctg tactacaatg
     2341 cttggaaaga tatcaagaac aaagaatgat acgattgaaa ataaactgat tcaagaacta
     2401 ctgggcagtg attgaggttt gggtgacatc aaagaaaagt tagattaatt ctaaagaatt
     2461 gagtatttct atgttaaaaa aatatccttg tacgatgcaa catgatcagt cagattgtgc
     2521 tgcggcagtt gtttcaacag ttcttttatc ttacaaaaag gaactatcaa tcatgaagat
     2581 tcgggaaatt attggtacag acatgtatgg aacgactgtc agtggtattg ttttaggtct
     2641 gaataagttg aattttacag taaaagctgt tcgagtagca ctggaagatt tgactccaaa
     2701 attaacattt cctgcgattc ttcaagttaa gaatgattta ggtcaaaatc attttgtggt
     2761 attacatagt ataaaggaga aaataaatgg aactcgtatt accaaataat tatgttgctc
     2821 ttgagcaaga ggagatgatg tatcttgatg ggggggctta tttaagcaag agtgcttgtc
     2881 aaggaatttg cgcagcttta gctatgagct caggaacttt tatagcattg actggagctg
     2941 cagttttaac aaaaaaacta ataaactata ttaaggttgg aggccttgga ggttggctta
     3001 ttggtgcagc agcaggtgta ttggctctta tctttttgat aaaaatttgt gtagatacta
     3061 cgataaaatt tttattcatc agattatttg acacaagtac tttaatgaag aggagaatat
     3121 tttttctttt cgcattggat gttatagctt acttattttt aggatatctt ttagtgattc
     3181 aaaaagcagg ctatttgttt tcggttttac ttcttttttc taatttttca gttccattta
     3241 tcaaagaaaa ggaattcgaa ttatttaaaa atagcaagta atagaaacat aggttaaatc
     3301 ttagaacggc ttaaaatcag atatgataaa gggaaatagt cttatctagg taactaagtg
     3361 ctacaaacac agtcgaggat cacttgttag gattacattt taatcatgct taataagaat
     3421 ggagaattcc tatggggatt gtattactct gaagtttcga ataccttagt tctcgtgatt
     3481 ttgagctatt ctaggtttag aatccttcag aatgtaaaat tttaagcagt caaagactta
     3541 gaatagcccg aaaacattta ttttatagca attctaggct tagaattgct catatatatg
     3601 tatttgagct gctcgatagt taggacagct ttattctgag aaatctgagc tatcaatgct
     3661 tcagaatagt atttcgccaa tggtttttat tgaagaggtg attgttttat gatagtctaa
     3721 aataaaactg atttaatgga gtaaatggag aatcgttaca tgaaacattt ttttgcagga
     3781 attggtgaga taaaacttgt tggctactta ttgtatatat gtgtaggact agcccctctt
     3841 tttcatatat atgtcatagg ttcggaactg agttttgtga aaatagtgtt atctattttg
     3901 ggagttatat ttgtaagtat gttaactatt gctcgaattt ataggacttt tttctataaa
     3961 gaatagtttg gacttgaact gtatctaaaa acagaggttg gtcgattatc agcctctttc
     4021 ttgtcgttga aaagagtgag atatattgac ttttgaaaaa aagatgtcat aattgagttg
     4081 ggataaacct aaaagtaata tagtagattg aatctggaat agtccgttat gactgttaaa
     4141 atatttctag aaattaattt gactttccta atcgatttgt tcacatcttg tttcaatcta
     4201 ctatactttc tcgttaaacc gttcggcaat tcatggtgac atggacacat ggtagtaagc
     4261 acactatgtg gcttggcaga gctaaaaact gttcccttgc aataagccta ataagcacaa
     4321 catagggagt tagagaagcc gactctaatc aaccactttg ggcaatagtg agaactgccc
     4381 tgtgcttttt tattttgaaa aaatatggag tttgtcgttg aaattacttg attgtatttt
     4441 ggactatcaa gaaagattca atggaaaaac atgtcaagta tcaacgaatt ataagtattt
     4501 agagattttc aaagtgaatt tttgcttgac tgatttacat catttatttg gcttacacaa
     4561 aatcacacga gattatgcta gtcaaacaaa acctgctatt caaactggtg tttttatttt
     4621 agaagatttt agaaatatcc tattgtacaa tgatgtaatc gaaaggatat ctttatatga
     4681 gtttataagt gatatcttct attctaagct aacaagttgt tgcatcgtag ctaaggattt
     4741 atctaaaaat actatgaaat tggacgtcat attttttgag gataaaaata aaagatccgc
     4801 agttttaggt ttacgaagag ataaaagcgg agtatttaaa ccagttactc tacattttac
     4861 aagcgctaag aaatatgcta aagttcgtaa aacagatgtg aaagaaatga aatggctata
     4921 aagattgtcc gcaggcttaa gcttgcgttt tttgttgttt aacgaaatta aaaaataagc
     4981 tctctcatcg gaaatgtttt tataggaatg aaggaagatg atgctatgtt taaaaagaga
     5041 atcgaaaagg gcaaaagtag tgtctttatc tttttggagt agaagcttat gatatagaaa
     5101 ttgtaataaa aatgtaatat aaaacatttg acaaatattt acggagggag tatacttaat
     5161 ataagtatag tctaaaaatg actatcagaa aagaggtaaa tttagatgaa taagaaaaaa
     5221 atgattttaa caagtctagc cagcgtcgct atcttagggg ctggttttgt tacgtcttcg
     5281 cctacttttg taagagcaga agaagctccc gtagctagtc agtctaaagc tgagaaagac
     5341 tatgatgcag cgaagaaaga tgctaagaat gcgaaaaaag cagtagaaga tgctcaaaag
     5401 gctttagatg acgcaaaagc tgctcagaaa aaatatgacg aggatcagaa gaaaactgaa
     5461 aagaaagctg ctgcggtaaa gaaaattgat gaagaacatc aggcggctaa tcttaaatct
     5521 cagcaagcat tagttgaatt cttagctgct caaagagaag gtaatcctaa aaagaaaaaa
     5581 gctgcgcaag ccaagttaga agaagcagaa aaagcagaaa aggagaagaa aaaggaattt
     5641 gataaagcac aggcagtggt agttcctgaa gcaactgagt tagctgagac taagaaaaaa
     5701 gcagacgaag ctaaagtaaa agaaccagaa cttactaaaa aactagaaga agctaaagca
     5761 aaatcagaag aagctgagaa aaaagctact gaagccaaac aaaaagtgga tgcagaacat
     5821 gctaaagaag tcgttcctca agctaaaatc gctgagttgg aaaatgaagt tcagaaacta
     5881 gaaaaagatc tcaaagagat tgatgaatct gactcagaag attatgttaa agaaggtctc
     5941 cgtgctcctc ttcaatctga attggatgcc aaacaagcta aactatcaaa acttgaagag
     6001 ttgagtgata agattgatga gttagacgct gaaattgcaa aacttgaaaa aaatgtagaa
     6061 gatttcaaaa actcaaacgg tgagcaagct gaacaatacc gtgctgcagc tgaagaagac
     6121 ttagctgcta aacaagctga attagaaaaa actgaagctg accttaagaa agcagttaat
     6181 gagccagaaa aaccagctcc agctccagaa actccagccc cagaagcacc agctgaacaa
     6241 ccaaaaccag cgccggctcc tcaaccagct cctgcaccaa aaccagagaa gccagctgaa
     6301 caaccaaaag cagaaaaaac agatgatcaa caagctgaag aagactatgc tcgtagatca
     6361 gaagaagaat ataaccgctt gactcaacag caaccgccaa aagcagaaaa accagctccc
     6421 gcaccaaaac cagagcaacc agctcctgca ccaaaaatag gctggaaaca agaaaacggt
     6481 atgtggtact tctacaatac tgatggttca atggcaacag gatggctcca aaacaatggt
     6541 tcatggtact acctcaacgc taatggttca atggtgacag gatggctcca aaacaatggt
     6601 tcatggtact acctcaacgc taatggttca atggcgacag gatggctcca aaacaacggt
     6661 tcatggtatt acctcaacgc taatggtgct atggcgacag gatggctcca aaacaacgat
     6721 tcatggtatt acctaaacgc tagcggcgct atggcaacag gttgggctaa agtcaacggt
     6781 tcatggtact acctcaacgc taatggtgct atggctacag gatggctcca atacaacggt
     6841 tcatggtact acctcaacgc taacggtgct atggcaacag gttgggttaa agtcaacggt
     6901 tcatggtact acctcaacgc taacggttca atggcaacag gttgggtgaa agatggagat
     6961 acctggtact atcttgaagc atcaggtgct atgaaagcaa gccaatggtt caaagtatca
     7021 gataaatggt actatgtcaa tggtttaggt gcccttgcag tcaacacaac tgtagatggc
     7081 tataaagtca atgccaatgg tgaatgggtt taagccgatt aaattaaatc atgttaagaa
     7141 catttgacat tttaattttg aaacaaagat aaggttcgat tgaataaatt tatgttcgta
     7201 ttctttaggt acctatctta tg
//
LOCUS       DAVLVP010000084         7218 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.084, whole genome shotgun sequence.
ACCESSION   DAVLVP010000084 DAVLVP010000000
VERSION     DAVLVP010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7218
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.084"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(2..847)
                     /locus_tag="VYN69_001678"
     CDS             complement(2..847)
                     /locus_tag="VYN69_001678"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEV1533914.1"
                     /translation="MYLIEILKSIFFGIVEGITEWLPISSTGHLILAEEFIQYQNQNE
                     AFMSMFNVVIQLGAILAVMVIYFNKLNPFKPTKDKQEVRKTWRLWLKVLIATLPLLGV
                     FKFDDWFDTHFHNMVSVALMLIIYGVAFIYLEKRNKACAIEPSVTELDKLPYTTAFYI
                     GLFQVLALLPGTSRSGATIVGGLLNGTSRSVVTEFTFYLGIPVMFGASALKIFKFVKA
                     GELLSFGQLFLLLVAMGVAFAVSMVAIRFLTSYVKKHDFTLFGKYRIVLGSVLLLYSF
                     VRLFV"
     gene            1103..2130
                     /locus_tag="VYN69_001679"
                     /pseudo
     CDS             1103..2130
                     /locus_tag="VYN69_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841243.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2431..4320)
                     /locus_tag="VYN69_001680"
     CDS             complement(2431..4320)
                     /locus_tag="VYN69_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HEV1533915.1"
                     /translation="MKKTFFLLVLGLFCLLPLSVFAIDFKINSYQGDLYIHADNTAEF
                     RQKIVYQFEEDFKGQIVGLGRAGKMPSGFDIDPHPKIQAAKNGAELADVTSEVTEEAD
                     GYTVRVYNPGQEGDIVEVDLVWNLKNLLFLYDDIAELNWQPLTDSSESIEKFEFHVRG
                     DKGAEKLFFHTGKLFREGTIEKSNLDYTIRLDNLPAKRGVELHAYWPRTDFASARDQG
                     LKGNRLEEFNKIEDSIVREKEQSKQLLTWVLPSILSISLLLSVCFYFIYRRKTTPSVK
                     YAKNHRLYEPPMELEPMVLSEAVYSTSLEEVSPLVKGAGKFTFDQLIQATLLDVIDRG
                     NVSIISEGDAVGLRLVKEDGLSSFERDCLNLAFSGKKEETLSNLFADYKVSDSLYRRA
                     KVSDEKRIQARGLQLKSSFEEVLNQMQEGVRKRVSFWGLPDYYRPLTGGEKALQVGMG
                     ALTILPLFIGFGLFLYSLDVHGYLYLPLPILGFLGLVLSVFYYWKLRLDNRDGVLNEA
                     GAEVYYLWTSFENMLREIARLDKAELESIVVWNRLLVYATLFGYADKVSHLMKVHQIQ
                     VENPDINLYVAYGWHSMFYHSSAQMSHYASVANTASTYSVSSGSGSSGGGFSGGGGGG
                     SIGAF"
     gene            4458..6023
                     /locus_tag="VYN69_001681"
     CDS             4458..6023
                     /locus_tag="VYN69_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEV1533916.1"
                     /translation="MRKIYLSIFTSLLLMLGLVNVAQADEYLRIGMEAAYAPFNWTQD
                     DDSNGAVKIDGTNQYANGYDVQIAKKIAKDLGKEPLVVKTKWEGLVPALTSGKIDMII
                     AGMSPTAERKQEIAFSSSYYTSEPVLLVKKDSAYASAKSLDDFNGAKITSQQGVYLYD
                     LIAQIPGAKKETAMGDFAQMRQALEAGVIDAYVSERPEALTAEAANSKFKMIQVEPGF
                     KTGEEDTAIAIGLRKDDTRISQINASIETISKDDQVALMDRMIKEQPAEATTTEETSS
                     SFFSQVAKILSENWQQLLRGAGITLLISIVGTITGLIIGLAIGVFRTAPLSENKAIYG
                     LQKLVGWILNVYIEIFRGTPMIVQSMVIYYGTAQAFGINLDRTLAAIFIVSINTGAYM
                     TEIVRGGILAVDKGQFEAATALGMTHNQTMRKIVLPQVVRNILPATGNEFVINIKDTS
                     VLNVISVVELYFSGNTVATQTYQYFQTFTIIAVIYFVLTFTVTRILRFIERRMDMDTY
                     TIGANQMQTEDLK"
     gene            6023..6787
                     /locus_tag="VYN69_001682"
     CDS             6023..6787
                     /locus_tag="VYN69_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1533917.1"
                     /translation="MTQAILEIKHLKKSYGQNEVLKDISLTVHKGEVISIIGSSGSGK
                     STFLRSINLLETPTDGQILYHGQNVLEKGYDLTQYREKLGMVFQSFNLFENLNVLENT
                     IVAQTTVLKRERTEAEEIAKENLEKVGMGERYWQAKPKQLSGGQKQRVAIARALSMNP
                     DAILFDEPTSALDPEMVGEVLKIMQDLAQEGLTMIVVTHEMEFARDVSHRVIFMDKGV
                     IAEEGKPEDLFTNPKEDRTKEFLQRYLILNENQRAN"
     gene            complement(6986..7201)
                     /locus_tag="VYN69_001683"
     CDS             complement(6986..7201)
                     /locus_tag="VYN69_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002893653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533918.1"
                     /translation="MAKSNFEKVESVVGWVRDKKITGYRISKETNAREMSIIALAQGR
                     AKVKNISFETALGLIDFYEKNHEKFED"
BASE COUNT         2271 a         1617 c         1322 g         2008 t
ORIGIN      
        1 cttatacaaa taaacggaca aaactgtaaa gtagcaaaac actaccaagc acgatacggt
       61 atttaccaaa aagggtgaag tcgtgttttt tcacatagct ggtcaagaag cgaatagcca
      121 ccatgctgac cgcaaaagct actcccatcg cgaccaagag caaaaacaat tgcccaaagc
      181 tcaagagttc tccggctttc acaaatttga aaatctttaa ggcactagct ccaaacataa
      241 cgggaatccc aagatagaag gtaaattctg tcacaactga acgactggtt ccatttaaca
      301 aaccaccgac aatcgttgca cctgaacggc tagtccctgg taaaagagca agaacttgga
      361 agagtccgat atagaaagcg gtcgtataag gaagcttgtc caactctgtt acacttggct
      421 cgatagcaca cgctttattg cgcttttcca aatagatgaa ggcaaccccg tagataatca
      481 acatgagagc aactgaaacc atgttatgga agtgggtatc aaaccaatca tcaaatttaa
      541 agacaccaag taaaggtaaa gtagcaatca agaccttcaa ccatagtctc caagtcttac
      601 gaacttcctg tttgtcctta gtcggtttaa aaggattgag cttgttaaaa taaatcacca
      661 taactgctaa aatagcacca agctgaatca cgacattaaa catggacata aaggcttcat
      721 tttgattttg gtattggata aactcctctg ctaaaatcaa gtgacctgta ctggaaatcg
      781 gcaaccattc cgtaattcct tcaacaatcc cgaagaagat agattttaaa atttcaataa
      841 gatacatata tttctcctta tattatattt aacgcagtta gctactgcca aagcccaagt
      901 ggtatacttg gaataagcca ctgtggatta gttcatttcc tccaatcgct tgacgatttc
      961 cgaaaggatc taaccacagc tttttgtttc aattggtaat gagtttgata acgcatagac
     1021 gttttatctc gtctaaggta acaaggcaaa aagttcgtgg ataaccaagt agacttgaaa
     1081 tactatatct ggaggtactc ccatgcttta tgtgggcatt gatgtcgcta aaaataaaca
     1141 cgatgttaca gccttgaatg ttcgaggaaa actgttctta aaccactcac tttttcaaat
     1201 aataaagctg gttttgaact attagatctg tctcttcgac agctcaacca agactgtctc
     1261 atcgctcttg aagataccgg acattatgcc tttaatctac tgaacttcct gcatgaacaa
     1321 ggatataaag tttacaccta cactcctttg ctcatcaagg aatttgccaa atcattatca
     1381 cttcgcaaga ctaaaacgga taagaaagac gctcatggca tcgctcttaa acttctttct
     1441 gaccccaatc gtgaacaatt tcaacacgat aatcggcaag tagaactaaa aatactggct
     1501 agacatattc atcgtctcaa gaaaaaacag tctgattgga aagtacaata cactcgttgt
     1561 cttgatatca tctttcctga gttggataaa atcgttggaa agcattcaga atatacctac
     1621 caactcttga catgctaccc taatcctcag aaaaggcttg aggcaggatt tgataagctg
     1681 atagaaatta agcgattgac cgcttctaaa attcaggata tcctctcagt cgcacctcgt
     1741 tctatcggaa caacatctcc tgctcgtgaa ttcgaaatca tcgaaaacat caaacattac
     1801 aagaggctca ttgacaaggc ggaaaaatgt gtcaatgact tgatggctga gttcaactcg
     1861 gtcatcacga cggttactgg gattgggaat cgtttagggg cggtcatttt agccgagatt
     1921 caaaatattc atgcctttga taatcctgct caattacaag ctttcgctgg actggattct
     1981 tctatttatc agtcaggtca gattgattta gctggaagaa tggtcaaacg gggttctcct
     2041 catctgcggt gggcactcat acaagctgcc aaagcatgcg ctcgcttttc acctgctttt
     2101 aaggcctatc ttaagactaa gttagaataa gaaaaacatt acaatgtagc catcatccac
     2161 cttgcaaaaa aacttatccg aaccctgttt tatatcctaa aaaagagctg ccatttgacg
     2221 aacaaaaagt gatgtaattt ctgaaatcaa cacaggatta cctttgaaaa aatggacttt
     2281 ttcaagggct ttttgccgtg ctctaaaatc atcgccaaat catcaaattg aaataagaaa
     2341 tagtttagat aaaattattg acttttctta tagtttgtct ttcttctatc ttccattata
     2401 tcatactttt tcagtctatg gtagctctct ttaaaaggct ccgatactgc cgccacctcc
     2461 gcctccagag aagccaccgc cagaacttcc acttccagaa gatacggagt aggtacttgc
     2521 tgtatttgcg acactagcat aatggctcat ttgcgcgctt gaatgataaa acatactgtg
     2581 ccagccataa gctacataga gattgatatc tggattttca acttgaatct gatgaacctt
     2641 catcaaatga ctaaccttgt ccgcatagcc aaataaggtt gcatagacca agagacgatt
     2701 ccaaacaaca atactttcca attcagcctt atccagtcgt gcaatctcac gtaacatatt
     2761 ttcaaaactg gtccagagat agtagacctc agctcccgct tcatttagaa caccatcacg
     2821 attatctagt cgaagcttcc aataatagaa aacagacaaa actaacccta gaaaaccaag
     2881 tattggcaaa gggaggtaaa gatagccatg aacgtctaaa ctgtacaaga acaaaccaaa
     2941 tccgataaat aggggcagga tagtcaaggc acccataccc acttgcaagg ccttttcccc
     3001 accagttaaa ggacgataat aatctgggag cccccagaag gaaactcgtt ttctcactcc
     3061 ttcttgcatc tggttcaata cctcttcaaa agaagatttg agttgaagcc ctcttgcttg
     3121 aatccgtttt tcatcagaaa ctttggctct acgataaaga ctatcagata ccttgtaatc
     3181 cgcaaacaaa ttggaaagag tttcttcttt tttacctgaa aaggctagat ttagacagtc
     3241 tctctcaaag cttgacaaac catcttcttt tactagcctc aaaccaactg catctccttc
     3301 tgaaatgata gagacattcc cacggtctat cacatctagc aaggtagctt gaataagttg
     3361 atcaaaggtg aattttccag ctcccttgac caagggactc acttcctcca aggaggtcga
     3421 gtagactgct tctgataaaa ccataggctc taattccatt ggtggttcat agagacgatg
     3481 atttttggca tatttgactg aaggagtggt ctttcttcta taaataaaat agaagcagac
     3541 actcaataac aaggagatgg aaaggatcga agggaggacc caagtaagta gttgtttact
     3601 ttgctctttt tctctaacaa tcgagtcttc tatcttatta aactcttcta atcgattccc
     3661 tttcaatccc tgatccctag cgctagcaaa atcggtccga ggccaatagg catgcaactc
     3721 aactccacgc ttagccggaa gattgtctaa acggatagta taatcaaggt tactcttttc
     3781 aatcgttccc tctctaaaaa gtttccctgt atggaaaaag agtttttcag cccccttgtc
     3841 tccccttaca tgaaattcaa acttttcaat agactctgaa ctatctgtca gaggttgcca
     3901 atttaattca gcgatatcat cataaaggaa aagtaaattt tttaagttcc agacgaggtc
     3961 aacttcaact atgtcgccct cctgacctgg attatagact ctcacagtat aaccatccgc
     4021 ttcttctgtt acttcgctag tcacatctgc tagttctgca ccgtttttcg cggcctgaat
     4081 ctttggatga gggtcaatgt caaacccgct aggcatctta ccagcacgtc caagtcccac
     4141 gatttggccc ttaaagtcct cctcaaactg gtaaactatc ttctgtctaa actctgccgt
     4201 attatctgca tgaatataca aatccccttg ataagagttt atcttgaaat caatggcaaa
     4261 aacagagagt ggaagaaggc aaaacaagcc taacaccagt aagaaaaaag tttttttcat
     4321 caactaagcc ccctttttat ctttcttatc attatagcat tttttcgtaa gtaaaggctt
     4381 acttatgttg aaaaattacg catttttcga taaaatagga gacagttatt ttttaataga
     4441 tcagaatagg agaaatcatg agaaaaatat acttatctat tttcacaagt ctcttgctga
     4501 tgctaggact tgtcaatgtt gctcaagccg atgaatattt acgcatcggg atggaagcag
     4561 catatgctcc ctttaactgg acccaggatg atgatagcaa cggagctgtc aaaatcgatg
     4621 gaaccaacca gtacgccaac ggatacgatg ttcaaatcgc caagaaaatc gctaaggact
     4681 taggtaaaga acctttggtt gttaaaacca agtgggaagg tctagtccct gcccttactt
     4741 ctggtaagat tgacatgatt atcgcaggta tgagtccaac tgcagaacgc aaacaagaaa
     4801 ttgccttttc gagcagttac tacactagcg aaccagtttt gcttgtcaaa aaagattctg
     4861 cctacgcaag tgctaaatct ttggatgact ttaacggtgc aaaaatcact tctcaacaag
     4921 gggtctacct ctacgacttg attgcacaaa tcccaggtgc taaaaaagaa acagccatgg
     4981 gagacttcgc tcaaatgcga caagctcttg aggctggtgt cattgatgct tatgtttctg
     5041 aacgtccaga agcactgact gctgaagctg cgaactctaa gttcaagatg attcaagtag
     5101 aacctggttt caaaactggg gaagaagata cagctatcgc cattggactt cgtaaagatg
     5161 acactcgtat tagccaaatc aatgccagca ttgaaaccat ttcaaaggac gaccaagttg
     5221 ccctgatgga tcgtatgatc aaggaacaac ctgccgaagc tacaacaact gaagagacta
     5281 gcagtagttt ctttagccaa gttgctaaaa ttctttctga aaactggcaa caactcttgc
     5341 gtggtgctgg tatcactctt ttaatctcta tcgtcggaac catcacaggt ctcattattg
     5401 gacttgccat tggtgttttc cgtactgctc ctctctctga aaacaaagcc atttacggcc
     5461 tacaaaaact agtcggctgg atcctcaacg tctacattga aattttccgt ggtacgccaa
     5521 tgattgttca atcgatggtt atctactatg gaactgccca agctttcggg atcaaccttg
     5581 accgtacact ggctgctatc ttcatcgttt caatcaatac cggtgcctac atgactgaaa
     5641 tcgtccgtgg tggtatccta gcagttgaca agggacaatt tgaagctgcg actgctcttg
     5701 gtatgaccca taaccagacc atgcgtaaga ttgtcctacc tcaggtagtc cgtaacatcc
     5761 tacctgcaac tggtaatgaa tttgtcatca atatcaaaga tacatctgta ttgaacgtta
     5821 tctctgttgt cgaactttat ttctcaggaa ataccgtggc aacacaaacc tatcaatact
     5881 tccagacatt tacaatcatc gccgtgattt actttgtcct caccttcacc gtaacacgta
     5941 tcctacgatt tatcgagcgc cgaatggaca tggataccta tactataggt gctaaccaaa
     6001 tgcaaacgga ggatttgaaa taatgacaca agcaatcctt gaaattaaac acctcaaaaa
     6061 atcctatgga caaaacgaag tgctaaaaga catttcactc actgtccaca agggagaggt
     6121 catctctatc atcggaagct ctggaagcgg aaaatcgacc ttcctacgct ccattaacct
     6181 acttgaaaca ccaactgatg gacaaatcct ttatcatgga caaaacgtcc tcgaaaaagg
     6241 ctatgacctc acgcaatacc gtgaaaagtt ggggatggtt ttccaatcct ttaacctctt
     6301 tgaaaatctc aatgttcttg aaaacacaat cgtcgctcag acaactgtcc ttaaacgaga
     6361 acgcacagaa gctgaagaaa ttgccaaaga aaacctggaa aaggtcggca tgggagaacg
     6421 ctactggcaa gcgaaaccaa aacaactctc aggtggtcaa aaacaacgtg tggccatcgc
     6481 tcgtgccctt tcaatgaatc ctgacgccat tctctttgat gaaccaacat cagctctcga
     6541 tccagaaatg gttggagagg tcctcaaaat catgcaagat ctggctcagg aaggcttgac
     6601 catgattgtc gtaacccatg aaatggaatt tgcccgtgat gtctctcacc gtgttatctt
     6661 tatggataag ggtgtgattg ctgaagaagg taaaccagaa gatctcttca ctaatcctaa
     6721 agaagaccgt acaaaagaat tccttcaacg ttatctcata ctcaatgaaa atcaaagagc
     6781 aaactaggca gctagccgca ggttgctcaa aacactgttt tgaggttgca gatagagctg
     6841 acgtggtttg aagagatttt cgaagagtat caaataaaaa gaaaaggctg catcaaccgt
     6901 gcagtctttt tgctgtcctc aaaatgaaaa ggcagattct ctatctctac tacttgaagg
     6961 tcaagaatcc gccgttatcc aaagattaat cttcaaattt ttcatgattt ttttcataga
     7021 aatcaattag gcctagggct gtttcaaatg aaatattttt tacttttgca cgaccctgcg
     7081 ccagagcaat gatagacatt tcacgcgcat tcgtttcttt agagatacgg tagcctgtga
     7141 ttttcttatc acgaacccag ccaacaactg attctacttt ttcaaagttt gacttagcca
     7201 tgtttttctc ctattttc
//
LOCUS       DAVLVP010000085         7178 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.085, whole genome shotgun sequence.
ACCESSION   DAVLVP010000085 DAVLVP010000000
VERSION     DAVLVP010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7178
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.085"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            226..6642
                     /locus_tag="VYN69_001684"
     CDS             226..6642
                     /locus_tag="VYN69_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000750039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HEV1533919.1"
                     /translation="MKKSTVLSLTTAAVILAAYAPNEVVLADTSSSEDALSISDKEKV
                     VVDKETENKEKHKDIHSAMETSQDFKEKKIAVIKEKEVVSKTPVIDTKTSNEENSNKS
                     QGDHTDSFVNKNTENPKKEDKVVYIAEFKDKESGEKAIKELSSLKNTKVLYTYDRIFN
                     GSAIETIPDNLDKIKQIEGISSVERAQKVQPMMNHARKEIGVEEAIDYLKSINAPFGK
                     NFDGRGMVISNIDTGTDYRHKAMRIDDDAKASMRFKKEDLKGTDKNFWLSDKIPHAFN
                     YYNGGKITVEKYDDGRDYFDPHGMHIAGILAGNDTEQDIKNFNGIDGIAPNAQIFSYK
                     MYSDAGSGFAGDETMFHAIEDSIKHNVDVVSVSSGFTGTGLVGEKYWQAIRALRKAGI
                     PMVVATGNYATSASSSSWDLVANNHLKMTDTGNVTRTAAHEDAIAVASAKNQTVEFDK
                     VNIGGQSFKYRNIGAFFDKNKITTNEDGTKAPSKLKFVYIGKGQDQDLIGLDLRGKIA
                     VMDRIYTKDLKNAFKKAMDKGARAIMVVNTVNYYNRDNWTELPAMGYEADEGTKSQVF
                     SISGDDGVKLWNMINPDKKTEVKRNNKEDFKDKLEQYYPIDMESFNSNKPNVGDEKEI
                     DFKFAPDTDKELYKEDIIVPAGSTSWGPRIDLLLKPDVSAPGKNIKSTLNVINGKSTY
                     GYMSGTSMATPIVAASTVLIRPKLKEMLEKPVLKNLKGDDKIDLTSLTKIALQNTARP
                     MMDATSWKEKSQYFASPRQQGAGLINVANALRNEVVATFKNTDSKGLVNSYGSISLKE
                     IKGDKKYFTIKLHNTSNRPLTFKVSASAVTTDSLTDRLKLDETYKDEKSPDGKQIVPE
                     IHPEKVKGANITFEHDTFTIGANSSFDLNAVINVGEAKNKNKFVESFIHFESVEEMEA
                     LSSNGKKTDFQPSLSMPLMGFAGNWNHEPILDKWAWEEGSKSKTMEGYDDDGKPKIPG
                     TLNKGIGGEHGIDKFNPAGVIQNRKDKNRTSLDQDPDLFAFNNQGVHAESTSGSKIAN
                     IYPLDSNGNPQDAQLERGLTPSPLVLRSAEEGLISIVNTNKEGENQKDLKVVSREHFI
                     RGILNSKGNDAKGIKSSKLKVWGDLKWDGLIYNPRGREENAPESKDNQDPATKIRGQF
                     EPIAEGQYFYKFKYRLTKDYPWQVSYIPVKIDNTAPKIVSVDFSNPEKIKLITKDTYH
                     KVKDQYKNETLFARDQKEHPEKFDEIANEVWYAGAALVNEDGEVEKNLEVTYAGEGQG
                     RNRKLDKDGNTIYEISGAGDLRGKIIEVIALDGASNFTKIHRIKFANQADEKGMISYY
                     LVDPDQDSSKYQKLGEIPESKFKNLKNVKDDSLNKETAEVENNLLVDNQSIEGKSLFN
                     IHKTISTIRDFENKDLKKLIKKKYKQEDDFVNGGTRTVERDYKYDDKGNIIAYDDGTD
                     LEYETEKLDEIKSKIYGVLSPSKDGHFEILGKISNVSKNAKVYYGNNYKSIEIKATKY
                     DFHSKTMTFDLYANINDIVDGLAFAGDMRLFVKDNDQKKAEIKIRMPEKIKETKSEYP
                     YVSSYGNVIELGEGDLSKNKPDNLTKMESGKIYSDSEKQQYLLKDNIILRKGYALKVT
                     TYNPGKTDMLEGNGVYSKEDIAKIQKANPNLRALSETTIYADSRNVEDGRSTQAVLMS
                     ALDGFNIIRYQVFTFKMNDKGEAIDKDGNLVTDSSKLVLFGKDDKEYTGEDKSNVEAI
                     KEDGSMLFIDTKPVNLSMDKNYFNPSKSNKIYVRNPEFYLRGKISDKGGFNWELRVNE
                     SVVDNYLIYGDLHIDNTRDFNIKLNVKDGDIMDWGMKDYKANGFPDKVTDMDGNVYLQ
                     TGYSNLNAKAVGVHYQFLYDNVKPEVNIDPKGNTSIEYADGKSVVFNINDKRNNGFDG
                     EIQEQHIYINGKEYTSFNDIKQIIDKTLNIKIVVKDFARNTTVKEFILNKDTGEVSEL
                     KPHRVTVTIQNGKEMSSTIVSEEDFILPVYKGELEKGYQFDGWEISGFEGKKDAGYVI
                     NLSKDTFIKPVFKKIEEKKEEENKPTFDVSKKKDNPQVNHSQLNESHRKEDLQREDHS
                     QKSDSTKDVTATVLDKNNISSKSTTNNPNKLPKTGTASGAQTLLAAGIMFIVGIFLGL
                     KKKNQD"
BASE COUNT         2818 a          990 c         1372 g         1998 t
ORIGIN      
        1 taatcttatt ttttaacgtg tgctaaaaaa taaattaatg catgttaatt tttcttgaat
       61 taaatttttt taaatgatat actgttttta tggagagata acaattatat ataaatgtac
      121 tactctattt cctagataat acttagtaaa actttttata acaaaggcta gcaaagttaa
      181 agtttattat ctattggaag ttaaataaat atgtaaggaa ttaaaatgaa aaaaagtaca
      241 gtattgtcat taaccacagc tgcagttatt ttagcagcct atgcccctaa tgaggtagtc
      301 ttagcagaca catctagctc tgaagatgct ttaagcatct ctgataaaga aaaagtagta
      361 gtagataagg aaacagaaaa taaagagaaa cataaagata tccatagtgc tatggaaact
      421 tcacaggatt ttaaagagaa gaaaatagca gtcattaagg aaaaagaagt tgttagtaaa
      481 actcctgtga tagacactaa aactagcaat gaagaaaatt ccaataaatc ccaaggagat
      541 catacggact catttgtgaa taaaaacaca gaaaatccca aaaaagaaga taaagttgtc
      601 tatattgctg aatttaaaga taaagaatct ggagaaaaag caatcaagga actatccagt
      661 cttaagaata caaaagtttt atatacttat gatagaattt ttaacggtag tgccatagaa
      721 acaattccag ataacttgga caaaattaaa caaatagaag gtatttcatc ggttgaaagg
      781 gcacaaaaag tccaacccat gatgaatcat gccagaaagg aaattggagt tgaggaagct
      841 attgattacc taaagtctat caatgctcca tttgggaaaa attttgatgg tagaggtatg
      901 gtcatttcaa atatcgatac tggaacagat tataggcata aggctatgag aatcgatgat
      961 gatgctaaag cctcaatgag atttaaaaaa gaagacttaa aaggaactga taaaaatttc
     1021 tggttgagtg ataaaatccc tcatgcgttc aattattata atggtggcaa aatcactgta
     1081 gaaaaatatg atgatggaag ggattatttt gacccacatg ggatgcatat tgcagggatt
     1141 cttgctggaa atgatactga acaagatatc aaaaacttta acggcataga tggaattgcg
     1201 cctaatgcac aaattttctc ttataaaatg tattctgacg caggatctgg gtttgcgggt
     1261 gatgaaacaa tgtttcatgc tattgaagat tctatcaaac acaacgttga tgttgtttcg
     1321 gtatcatctg gttttacagg aacaggtctt gtaggtgaga aatattggca agctattagg
     1381 gcattaagaa aagcaggcat tccaatggtt gtcgctacgg gtaactatgc gacttctgct
     1441 tcaagttctt catgggattt agtagcaaat aatcatctga aaatgaccga cactggaaat
     1501 gtaacacgaa ctgcagcaca tgaagatgcg atagcggtcg cttctgctaa aaatcaaaca
     1561 gttgagtttg ataaggttaa cattggagga caaagtttta aatacagaaa tataggggcc
     1621 tttttcgata agaataaaat cacaacaaat gaagatggaa caaaagctcc tagtaaatta
     1681 aaatttgtat atataggcaa ggggcaagac caagatttga taggtttgga tcttaggggc
     1741 aaaattgcag taatggatag aatttataca aaggatttaa aaaatgcttt taaaaaagct
     1801 atggataagg gtgcacgcgc cattatggtt gtaaatactg taaattacta caatagagat
     1861 aattggacag agcttccagc tatgggatat gaagcggatg aaggtactaa aagtcaagtg
     1921 ttttcaattt caggagatga tggtgtaaag ctatggaaca tgattaatcc tgataaaaaa
     1981 actgaagtca aaagaaataa taaagaagat tttaaagata aattggagca atactatcca
     2041 attgatatgg aaagttttaa ttccaacaaa ccgaatgtag gtgacgaaaa agagattgac
     2101 tttaagtttg cacctgacac agacaaagaa ctctataaag aagatatcat cgttccagca
     2161 ggatccacat cttgggggcc aagaatagat ttacttttaa aacccgatgt ttcagcacct
     2221 ggtaaaaata ttaaatccac gcttaatgtt attaatggca aatcaactta tggctatatg
     2281 tccggaacta gtatggcgac tccaatcgta gcagcttcta ctgttttgat tcgaccaaaa
     2341 ttgaaggaaa tgcttgaaaa acccgtatta aaaaatctta aaggtgatga taaaatagac
     2401 cttacaagtc ttacaaaaat agccctacaa aatactgcaa gacctatgat ggatgcaact
     2461 tcttggaaag aaaaaagtca atactttgca tcacctagac agcagggggc ggggctaatt
     2521 aatgtggcca acgctttgag aaatgaagtt gtagcgactt tcaaaaacac ggattctaaa
     2581 ggtttggtaa attcatatgg ttccatttct cttaaagaaa taaaaggtga taaaaaatac
     2641 tttacaatta agcttcacaa tacatccaac aggcctttaa cctttaaagt ttcagcatca
     2701 gcggtaacta cagattctct aactgacaga ctaaaactgg atgaaacata taaagatgaa
     2761 aaatctccag atggtaagca aattgttcca gaaattcatc cagaaaaagt caaaggggca
     2821 aatattacat ttgagcatga cactttcact ataggggcaa attctagctt tgatttaaat
     2881 gcggttataa atgttgggga ggctaaaaat aaaaataaat ttgtagaatc atttattcat
     2941 tttgagtcag tagaagaaat ggaagcatta agctccaatg gcaagaaaac cgacttccaa
     3001 ccatctttat caatgcccct aatgggattt gctgggaatt ggaaccacga accaatcctt
     3061 gataaatggg cttgggaaga aggatcaaaa tcaaaaacaa tggaaggtta tgatgatgac
     3121 ggtaaaccaa aaattccagg aaccttaaat aaggggattg gtggagaaca tggtatagat
     3181 aaatttaatc cagcaggagt tatccaaaat agaaaagata aaaatagaac atctctagat
     3241 caagatccag acttatttgc ctttaacaac caaggtgttc atgcagaatc cactagtggt
     3301 tctaagattg ctaacattta tcctttagat tcaaatggaa atcctcaaga tgctcaacta
     3361 gaaagaggac taactccatc tccacttgtt ctaagaagtg cagaagaagg actaatttca
     3421 attgtaaaca ccaacaaaga aggagaaaac caaaaagacc ttaaagttgt atcaagagag
     3481 cacttcataa gaggaattct aaattctaag ggaaatgacg ctaagggtat caaatcatcc
     3541 aaactaaaag tttggggtga cttgaaatgg gatggactca tctataaccc aagaggtaga
     3601 gaagaaaatg ccccagaaag taaggataac caagatccag ctactaagat acgaggacaa
     3661 tttgaaccga ttgcggaagg tcaatatttc tataaattta aatatagatt aactaaagat
     3721 tacccatggc aggtttccta tattcctgta aaaattgata acacagcccc taagattgtt
     3781 tcggttgatt tttcaaatcc tgaaaaaatt aagctaatca caaaggatac ttatcacaag
     3841 gtaaaagatc aatacaagaa tgaaacttta tttgctagag atcaaaaaga acatcctgaa
     3901 aaatttgatg agattgcaaa tgaggtgtgg tatgcaggag ctgctcttgt taatgaagat
     3961 ggagaggttg agaaaaatct tgaagtaact tatgcaggtg agggtcaagg aagaaatagg
     4021 aagcttgata aagatgggaa tactatctat gaaattagcg gagcaggaga tctaagagga
     4081 aaaatcatcg aagtaatcgc cctagatgga gcaagcaact ttaccaaaat ccataggatt
     4141 aaatttgcta atcaagctga tgaaaaaggg atgatttcct attatctagt agatcctgat
     4201 caggattcat ctaaatacca aaagcttggc gaaattcctg aatctaaatt taagaattta
     4261 aaaaatgtta aagatgatag cctaaacaaa gagacagctg aggtggaaaa taacctttta
     4321 gtagataatc agtctatcga aggaaaatct ctctttaata ttcataaaac tatttcaaca
     4381 attagagact ttgaaaataa agacctaaag aaactcatta aaaagaaata taagcaagaa
     4441 gacgactttg taaatggtgg aacaagaaca gtagagcgtg attataaata cgatgataaa
     4501 ggaaatataa tagcctacga tgatgggact gatctagaat atgaaactga gaaacttgac
     4561 gaaatcaaat caaaaattta tggtgttcta agtccgtcta aagatggaca ctttgaaatt
     4621 cttggaaaga taagtaatgt ttctaaaaat gccaaggtat attatgggaa taactataaa
     4681 tctatagaaa tcaaagcgac caagtatgat ttccactcaa aaacgatgac atttgatcta
     4741 tacgctaata ttaatgatat tgtggatgga ttagcttttg caggagatat gagattattt
     4801 gttaaagata atgatcagaa aaaagctgaa attaaaatta gaatgcctga aaaaattaag
     4861 gaaactaaat cagaatatcc ctatgtatca agttatggga atgtcataga attaggggaa
     4921 ggagatcttt caaaaaacaa accagacaat ttaactaaaa tggaatctgg taaaatctat
     4981 tctgattcag aaaaacaaca atatctgtta aaggataata tcattctaag aaaaggctat
     5041 gcactaaaag tgactaccta taatcctgga aaaacggata tgttagaagg aaatggagtc
     5101 tatagcaagg aagatatagc aaaaatacaa aaggccaatc ctaatctaag agccctttca
     5161 gaaacaacaa tttatgctga tagtagaaat gttgaagatg gaagaagtac ccaagctgta
     5221 ttaatgtcgg ctttggacgg ctttaacatt ataaggtatc aagtgtttac atttaaaatg
     5281 aacgataaag gggaagctat cgataaagac ggaaatcttg tgacagattc ttctaaactt
     5341 gtattatttg gtaaggatga taaagaatac actggagagg ataagtccaa tgtagaagct
     5401 ataaaagaag atggctccat gttatttatt gataccaaac cagtaaacct ttcaatggat
     5461 aagaactact ttaatccatc taaatctaat aaaatttatg tacgaaatcc agaattttat
     5521 ttaagaggta agatttctga taagggtggt tttaactggg aattgagagt taatgaatcg
     5581 gttgtagata attatttaat ctacggagat ttacacattg ataacactag agattttaat
     5641 attaagctga atgttaaaga cggtgacatc atggactggg gaatgaaaga ctataaagca
     5701 aacggatttc cagataaggt aacagatatg gatggaaatg tttatcttca aactggctat
     5761 agcaatttga atgctaaagc agttggagtc cactatcagt ttttatatga taatgttaaa
     5821 cccgaagtaa acattgatcc taagggaaat actagtatcg aatatgctga tggaaaatct
     5881 gtagtcttta acatcaatga taaaagaaat aatggattcg atggtgagat tcaagaacaa
     5941 catatttata taaatggaaa agaatataca tcatttaatg atattaaaca aataatagac
     6001 aagacactaa acattaagat tgttgtaaaa gattttgcaa gaaatacaac cgtaaaagaa
     6061 ttcattttaa ataaagatac gggagaggta agtgaattaa aacctcatag ggtaactgtg
     6121 accattcaaa atggaaaaga aatgagttca acgatagtgt cggaagaaga ttttatttta
     6181 cctgtttata agggtgaatt agaaaaagga taccaatttg atggttggga aatttctggt
     6241 ttcgaaggta aaaaagacgc tggctatgtt attaatctat caaaagatac ctttataaaa
     6301 cctgtattca agaaaataga ggagaaaaag gaggaagaaa ataaacctac ttttgatgta
     6361 tcgaaaaaga aagataaccc acaagtaaac catagtcaat taaatgaaag tcacagaaaa
     6421 gaggatttac aaagagaaga tcattcacaa aaatctgatt caactaagga tgttacagct
     6481 acagttcttg ataaaaacaa tatcagtagt aaatcaacta ctaacaatcc taataagttg
     6541 ccaaaaactg gaacagcaag cggagcccag acactattag ctgccggaat aatgtttata
     6601 gtaggaattt ttcttggatt gaagaaaaaa aatcaagatt aggacaaaag ctatagaaaa
     6661 aaatggttta tgtactgaga ttagatagtg aggtgatgac atagttttgt gaaaacagcc
     6721 atttataatt caattattta gtttacttca ctattgatac tatttggagt tattaatgga
     6781 cttagtttag ataactaatg aattgattga aagggttagt attgacaata ttggtcatat
     6841 tgattagaaa atagagtcta tcaaaattta aaggctaata gaggtgatga gacaatttca
     6901 aggaattaag tagcgtttgg ttgcttaatt cctttttatt tggaaggaag tttttcgtaa
     6961 ccgcccaata acgaagtcta ttggacggtt acgatatgat cactgacttc gtcagttcta
     7021 tctacaacct aaaaacagtg ttttgaacaa cctgcggcta gctttctagt ttgctctttg
     7081 attttcattg aatattagaa cagcaaaaat gcttggagta tttgtttgtg tgtttgtttt
     7141 tatataacaa actataaaca aaacaaaaat ataaaaaa
//
LOCUS       DAVLVP010000086         7014 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.086, whole genome shotgun sequence.
ACCESSION   DAVLVP010000086 DAVLVP010000000
VERSION     DAVLVP010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 7014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.086"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..676
                     /locus_tag="VYN69_001685"
     CDS             <1..676
                     /locus_tag="VYN69_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1533920.1"
                     /translation="RRLTMDEVNKLNSFDSLIENDIITSRGYKDQEYKRNGYYTIDLF
                     SPIYSALSGEKGTPGDLMGRRIAFELLAAKGYKEGMVPYISNQYEKDAKAAGSKIKSY
                     GKEVGLVTDKLVLEKVFNNRYSSWVEFKKAMYNERIAKFNNLISVSFNNPNVSWGRTN
                     RITITSIANLQNMINTAVNEDAEDFRAQLYPDTNSRVLKLKKAIYKAYLDQTNDFRRS
                     IFENKK"
     gene            720..6008
                     /locus_tag="VYN69_001686"
     CDS             720..6008
                     /locus_tag="VYN69_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000054776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1533921.1"
                     /translation="MSLLKKDKFSIRKIKGIVGSIFLGSFLFALSVVGVSTYHYLDYS
                     TLTQTERDQLKQGRPDESKESYALVYEKDALPNTGSSQSIMTVLGLLAIGSLIVIITK
                     DKKRKKIATFLIVGATGLVTLSTASALNLNANIHESGRDGVLQISGYRYVGYLELDDR
                     TVSSVSPASTVSPVEQPKVVTDKGEPEVQPALPEAVVTEKGEPEVQPALPEAVVTEKG
                     EPEVQPALPEAVVTEKGEPEVHEKPDYTQPIGANLVEPEVHEKLAYTEPVGTTGVDEN
                     GNLIEPPVNDIPEYTEPISTVSEVASEREELPSLHTDIRTETIPKTTIEESDPSKFIG
                     DNSVKQVSEDGERQIVTSYEELHGKKISESVETVTILKEMKPEIIVKGTKEKTAPVLT
                     LTKVTEDAMNRSANLNYELDNKDNAEISSIVAEIKDGDTVVKRVDLSKEKLTDAVQNL
                     DWFKDYKIATTMIYDRGQGSETSKLDERPLRLELKKVEIKNIVSTNLVKVNDDGTETP
                     SDFMNEKPSEEDVKKMYLKITSRDNKVTRLTVDSIEEVTEEGQKLYKITAEAQDLIQH
                     TDPTKVRNKYVYYIEKPHPKEDNVYYNFKDLVDAMNTDKNGTFKLGADLNATGVPTPK
                     KWYVDGDFRGTLKSVEGKHYTIHNTERPLFQNIIGGTVTKVNLGNVNINMPWADRIAP
                     IADTIKGGAKIEDVKVTGNVLGRNWVSGFIDKIDNQGTLRNVAFIGNVTSVGDGGQFL
                     TGIVGENWKGLVERAYVDANLIGKKAKAAGIAYWTQNSGDNHKVGVEGAVKKGIVKGT
                     IQVESPVEVGGAVGRLSHHGYIGEVVSMMKVKKGEIFYGSSDMNDDPYWVANNVRGNY
                     VVNGVSEGTISYARAKEHHRIKPISQSEADTKIMMLGITAQDFAINEPVVNRLNRLTR
                     KEDEYKSTQDYKVDRDLAYRNIEKLQPFYNKEWIVNQGNKLAEDSNLAKKEVLSVTGM
                     KDGQFVTDLSDIDKIMVHYADGTKEEMDVTKNADSKVKQVREYTIAGQNVVYTPNMVE
                     KDRNQLIQDIKDKLASVQLISPEVRALMDARKKPEENTDERKNGYIKDLYLEESFAET
                     KANLDKLVKSLVENADHQLNSDETAMKALVKKVDENKAKIMMALTYLNRYYDIKYGDM
                     TIKNLMMFKPDFYGKSVDLLDFLIRIGSSERNIKGDRTLDAYRDMIGGTIGKSELHGF
                     LDYNMRLFTNDTDLNDWFIHAAKNVYVSEPQTTNPDFVNKRHRAFDGLNNGVHNRMIL
                     PLLTLKNAHMFLISTYNTMAYSSFEKYGKYTEEARNEFKKEIDKVAHAQQTYLDFWSR
                     LALPSVRDQLLKSENRVPTPVWDNQNYSGIKGINRMGYDEKKVPIAPIRELYGPTWKF
                     HNTNWNMGAMASIFPNPNNNDQVYFMGTNMISPFGISAFTHETTHVNDRMLYFGGHRH
                     RQGTDVEAYAQGMLQTPSSIGHQGEYGALGLNMAYHRENDGDQWYNYDPDKLQTREDI
                     DRYMKNYNEALMMLDHVEADAVLPQLNGDNSKWFKKIDREMRRNLGDGLNNLVAPHQW
                     DNVRDLNQEESSKKLSSINDLIDNNFMTKHGNPGNGRYRPEDFRPNSAYVNVNMMAGI
                     YGGNTSQGAPGSLSFKHNAFRMWGYYGYDKGFTSYVSSKYQGEADKQNQGRLGDDFII
                     KKVSNNQFSNLEDWKKYWYHDVKSRAEKGFTEITIDGQTIHNYNELKDLFDKAVTEDL
                     KKAGNYSNTENLKSKVFKALLKNTDGFFNPLFKKDI"
     gene            6175..>7014
                     /locus_tag="VYN69_001687"
     CDS             6175..>7014
                     /locus_tag="VYN69_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1380-like element ISSpn5 family transposase"
                     /protein_id="HEV1533922.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFRLLTGYGTDYACKELSADAYFPKLLEGGQL
                     ASQPTLSRFLSRTDEETVHSLRCLNLELVEFFLQFHQLNQLIVDIDSTHFTTYGKQEG
                     VAYNAHYRAHGYHPLYAFEGKTGYCFNAQLRPGNRYCSEEADSFITPVLERFNQLLFR
                     MDSGFATPKLYDLIEKTGQYYLIKLKKNTVLSCLGDLSLPCPQDEDLTILPHSAYSET
                     LYQA"
BASE COUNT         2444 a         1145 c         1416 g         2009 t
ORIGIN      
        1 tcgcagatta acgatggatg aagttaataa attaaattct tttgatagtt taattgaaaa
       61 tgatattatt actagcagag ggtacaaaga ccaagaatat aagagaaatg gttattatac
      121 aattgattta ttttcaccga tttattcagc tcttagtgga gagaaaggga ctcctggtga
      181 tttaatggga cgtcgtatag cattcgaatt attagctgca aaaggatata aagagggcat
      241 ggtaccttat atctcaaatc aatatgaaaa agatgcaaaa gctgcgggaa gtaagattaa
      301 atcttatggt aaagaagtag gtttagttac agataaatta gtacttgaaa aggtatttaa
      361 taatcgttac tcatcttggg tagaatttaa aaaagcaatg tataatgagc gaatagctaa
      421 atttaataat ctaataagtg ttagctttaa taatccaaat gtaagttggg gaagaactaa
      481 tagaataaca ataactagca tagccaacct tcaaaatatg ataaatacgg ctgtcaatga
      541 agatgctgaa gattttagag cacaattata ccctgataca aatagtagag ttcttaaact
      601 gaaaaaagca atatataaag cctatcttga ccaaacaaat gattttagaa gatcaatttt
      661 tgagaataaa aaatagtgtt tactattagg aaataaaaag tgaatgtgga ggattagaat
      721 tgtcattatt aaaaaaagat aaattttcca ttaggaaaat aaagggtatt gttggttcga
      781 tattcttggg gagtttttta tttgcactgt cagttgttgg tgtatcgact tatcattact
      841 tagattatag tactttgaca caaactgaac gtgatcaact caaacaaggt agacctgatg
      901 aatcaaaaga gtcatatgct ttggtttatg aaaaagacgc attaccaaac acaggaagtt
      961 ctcaatctat tatgactgta ttgggtttgt tagctatcgg gagtcttatt gtgattatca
     1021 caaaagataa aaagaggaaa aaaatagcta catttttgat tgtaggggcg acaggtttag
     1081 ttacactatc aactgcttca gcacttaatt tgaatgctaa catccatgag tctggacgtg
     1141 atggtgtgtt gcaaatttct ggttacagat atgttggtta cttggagctt gatgatagaa
     1201 ctgtttcgtc ggtttctcca gcatcaactg tttctccagt tgagcaaccc aaagttgtga
     1261 ccgacaaagg cgaaccagaa gttcagccag cgttaccaga agcagttgta actgaaaaag
     1321 gggaaccaga agtacaacca gccttacccg aagcagttgt aactgaaaaa ggggaaccag
     1381 aagtacaacc agccttaccc gaagcagttg taactgaaaa aggggaacca gaagttcatg
     1441 agaagcctga ctatacgcaa ccaataggtg caaaccttgt tgagcctgaa gttcatgaga
     1501 aactagcata cactgaacct gtaggtacaa caggagtaga tgaaaatgga aacttgattg
     1561 agccacctgt taacgatatt ccagagtata ctgaaccaat atctacagtt tcagaagttg
     1621 catcagaaag agaagagttg ccttctttac ataccgatat tcgtacagag actattccta
     1681 aaaccactat agaagaatct gatcctagca aatttatagg cgataattct gttaaacaag
     1741 ttagtgagga tggcgaacgt caaattgtta ctagctatga agagttacat ggcaaaaaaa
     1801 tcagcgagtc agttgaaaca gtaacaattt tgaaagaaat gaagcctgaa attattgtaa
     1861 aaggtacaaa agaaaaaacg gctcctgttt taaccttgac caaagtaaca gaagatgcta
     1921 tgaatcgttc tgccaactta aattatgagc ttgataataa agataatgct gaaatatcaa
     1981 gtattgtagc tgaaattaaa gatggtgata cagtagttaa aagggttgat ttatctaaag
     2041 aaaaattaac tgacgctgta caaaatttgg attggtttaa agattataag attgcaacaa
     2101 caatgattta tgatcgtggt caaggttctg aaacgtctaa actggatgaa agacctctgc
     2161 gtttggagtt gaaaaaggtc gaaattaaaa atatcgtatc tactaattta gtgaaggtaa
     2221 atgatgatgg tactgaaaca cctagtgatt ttatgaatga aaaaccttca gaagaagatg
     2281 ttaaaaaaat gtacttaaaa atcactagtc gcgataataa ggtaactcgt ttaactgtag
     2341 actctattga agaagtgact gaagaaggtc aaaaacttta caaaataact gctgaagcac
     2401 aagatttgat tcaacacaca gacccaacga aggttcgtaa taagtatgtt tattatatag
     2461 aaaaacccca tcctaaagaa gataatgtct actataactt taaagattta gtagatgcta
     2521 tgaatactga taaaaatggt acatttaaac ttggtgcaga tttgaatgcc actggtgttc
     2581 caacacctaa gaaatggtat gttgacggcg actttagagg tacattgaag agtgtagagg
     2641 ggaaacatta tactatccac aacacggagc gtccgttatt ccagaatatt attggtggta
     2701 cagtaaccaa agtaaatctg ggaaatgtaa atatcaatat gccatgggca gaccgcatcg
     2761 caccgattgc tgacactatt aaaggtggcg ctaaaattga agatgtcaaa gtgactggta
     2821 acgtattagg tagaaactgg gtatctggat ttattgataa gattgacaac caaggtacac
     2881 tcagaaatgt agcctttatc ggaaatgtga catctgtagg tgatggtggt caattcttga
     2941 ccggtatcgt tggtgagaac tggaaaggac tagtcgaacg tgcgtatgtt gacgcaaatc
     3001 ttattggcaa gaaagccaag gctgctggta ttgcttattg gacacaaaac agtggtgaca
     3061 atcataaagt tggtgtcgaa ggtgcagtca agaaaggaat tgtgaagggt accattcagg
     3121 tagaaagtcc tgttgaagtc ggcggtgctg ttggacgttt gagtcatcat ggttatatcg
     3181 gggaagtcgt atctatgatg aaagttaaga agggtgaaat tttttatggt tctagtgata
     3241 tgaatgatga cccatattgg gtggcgaata atgtacgagg aaattatgtc gttaatggtg
     3301 tgagtgaagg tacgatatct tatgctcgtg ctaaagaaca ccatcgtatc aaaccaattt
     3361 cacagtcaga agcagatact aaaattatga tgttaggtat tacagcacaa gattttgcta
     3421 tcaacgaacc tgtggtcaat cgcttaaacc gtttaaccag aaaagaagat gaatataagt
     3481 caacccaaga ctataaagta gatcgagatc tggcttatcg taatattgag aaacttcaac
     3541 cattttataa caaagaatgg attgtcaacc aaggtaacaa acttgcagaa gattcaaact
     3601 tagctaagaa agaagtcctt tctgttacgg gtatgaagga tggacagttt gtaactgact
     3661 tatcagatat tgataagatt atggttcact atgctgatgg tactaaagaa gaaatggacg
     3721 tcactaagaa tgctgattct aaagtgaaac aagttcgtga atataccatc gctggacaaa
     3781 acgttgttta tacaccaaat atggttgaga aagaccgtaa ccaattgatt caagatatca
     3841 aagataagtt ggcgtcagtt caattgattt ctcctgaagt acgtgctttg atggatgcac
     3901 gtaagaaacc agaagaaaat actgatgaac gtaagaatgg ttatattaaa gacctttact
     3961 tagaagaaag ctttgctgaa acgaaagcta atcttgataa attggttaaa tccttagttg
     4021 aaaatgcaga tcatcaatta aatagtgacg aaacggctat gaaagcactt gttaagaagg
     4081 tggatgaaaa taaagctaaa atcatgatgg ctctgactta tctcaatcgt tactatgata
     4141 tcaagtatgg ggatatgact atcaagaact tgatgatgtt taaacctgat ttctatggta
     4201 aatcagttga ccttcttgat ttcttgattc gaattggatc aagtgagaga aatatcaaag
     4261 gtgacagaac tttggatgct tatcgtgata tgattggtgg aactattggt aagtctgaat
     4321 tacatggatt cttagattac aatatgcgtc tcttcactaa cgatactgat ttaaatgatt
     4381 ggttcatcca tgcagctaag aatgtatatg tatcagaacc tcaaacaacg aacccagatt
     4441 ttgttaataa acgacatcgt gcatttgatg gtttgaacaa cggtgttcat aatcgtatga
     4501 ttctacctct attaactctt aagaatgcac atatgttctt gatttcaact tacaatacaa
     4561 tggcttatag ttcttttgag aaatatggta agtatacaga agaagctaga aatgaattca
     4621 aaaaagaaat tgataaggtt gcacatgctc aacaaacata cttagacttc tggtcacgtt
     4681 tagcattgcc aagcgtacgt gaccaattac tcaagagtga gaatagggta ccaactcctg
     4741 tgtgggataa ccaaaactat agtggtatta aaggaataaa ccgtatgggt tatgatgaga
     4801 agaaagtacc aattgcgcca attcgtgagt tatatggtcc aacttggaaa ttccataata
     4861 ccaactggaa tatgggagct atggcatcta ttttcccaaa tcctaataac aatgaccaag
     4921 tttatttcat gggaacaaat atgattagtc cgtttggtat ttcagccttt actcatgaaa
     4981 ctacacacgt taatgataga atgctttact tcggtggtca tagacaccgt caaggtacag
     5041 acgtcgaagc ctatgctcaa ggtatgctac aaactccgtc aagtattggt caccaaggtg
     5101 aatatggtgc tttaggtttg aacatggctt atcatcgtga aaatgatggt gaccaatggt
     5161 ataactatga ccctgataag cttcaaactc gtgaagatat cgatcgatat atgaaaaact
     5221 ataatgaagc actgatgatg ctagatcatg tagaagcaga cgccgtactt ccacaattga
     5281 atggtgataa tagcaagtgg ttcaagaaaa ttgatagaga aatgcgcaga aatcttgggg
     5341 atggtttgaa taacttagta gcaccgcacc aatgggacaa cgttcgtgac ttaaatcaag
     5401 aagagagttc taagaaatta tcaagtatta acgatttgat tgataataat ttcatgacca
     5461 aacacggaaa tccaggtaac ggacgttata gaccagagga ttttagacca aactcagctt
     5521 acgtaaatgt caatatgatg gcaggaattt acggtgggaa tacgagtcaa ggtgctcctg
     5581 gttcattatc gttcaaacat aatgccttcc gtatgtgggg ttattatgga tatgacaaag
     5641 gtttcactag ttatgtatct agcaaatatc aaggtgaagc tgataaacaa aaccagggta
     5701 gattaggtga tgatttcatt atcaaaaaag tttcaaacaa ccaattctca aatcttgaag
     5761 attggaagaa atattggtat catgatgtga aatccagagc tgaaaaagga ttcacagaga
     5821 ttacaattga tggacaaacc attcacaact ataatgaact gaaagactta ttcgacaaag
     5881 ctgtcacaga agacctcaag aaagctggaa actactcaaa tactgagaat ttgaaatcca
     5941 aagtcttcaa agctcttctt aaaaacacag acggtttctt taatccattg ttcaaaaaag
     6001 acatttaaac cccaattatt caccccaaat ctaaaaacca tccagaatcc ttgccttagc
     6061 ttagatcctg gatggtttct ttttcaccca atgggtgttt tttactagac aaaaaagagt
     6121 ttccccttta tggtataagt gtagaaaaaa aacacaaaaa gaaaggaaac tcacatgaac
     6181 agtttaccaa atcatcactt ccaaaacaag tctttttacc aactatcttt cgatggaggt
     6241 catttaaccc agtatggtgg tcttatcttt tttcaggaac ttttttccca gttgaaacta
     6301 aaagagcgga tttctaagta tttagtaacg aatgaccaac gccgctactg tcgttattcg
     6361 gattcagata tccttgtcca gttcctcttt cgactgttaa caggctatgg aacggactat
     6421 gcttgtaaag aattgtcagc tgatgcctac tttccaaaat tgttggaagg agggcagctt
     6481 gcttcacagc caaccttatc ccgttttctt tccagaactg acgaggaaac agtccatagt
     6541 ttgcgatgcc tcaaccttga attggtcgaa ttctttttac agtttcacca gctaaaccaa
     6601 ctcattgtag atatcgattc tacccatttc acaacttatg gcaagcaaga aggtgttgct
     6661 tataacgccc actatcgtgc tcatggctat catcctcttt acgctttcga ggggaagaca
     6721 ggttattgtt tcaatgccca gcttcgtcct ggtaatcgtt attgttctga agaggcagac
     6781 agctttatca cacctgtttt agaacggttt aatcaacttc tctttcgaat ggatagtggc
     6841 tttgcgaccc caaaattata cgatttaatt gaaaaaacag ggcaatacta cctcataaaa
     6901 ctcaagaaaa atactgttct gagctgtctt ggagaccttt ccctcccttg cccacaggat
     6961 gaggacttaa ccatcttgcc ccactccgcc tactcagaaa ctctctatca agca
//
LOCUS       DAVLVP010000087         6998 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.087, whole genome shotgun sequence.
ACCESSION   DAVLVP010000087 DAVLVP010000000
VERSION     DAVLVP010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6998
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.087"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..463
                     /locus_tag="VYN69_001688"
     CDS             <1..463
                     /locus_tag="VYN69_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533923.1"
                     /translation="NFPDIVILDKNYQNKARKSIEIEEWKKLRGCVEIKSLKHDLITE
                     EKIKSTISNSFEHITGEMGQLIGDLLWYKKVIYTNGIEWRFLSLDDKEEIDNTIVQVV
                     NKRIETEEAGNSFDWWKNIKDLSFNYTDIYLSKDCIQEWDEFVKKVKEIEW"
     gene            502..603
                     /locus_tag="VYN69_001689"
                     /pseudo
     CDS             502..603
                     /locus_tag="VYN69_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677122.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group 2 glycosyl transferase"
     gene            complement(654..860)
                     /locus_tag="VYN69_001690"
     CDS             complement(654..860)
                     /locus_tag="VYN69_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEV1533924.1"
                     /translation="MKITLIPERCIACGLCQTYSDLFDYHDNGIVRFYDGPDQLEKEI
                     SPSQDVLEAVKNCPTRALIGNQEA"
     gene            912..1388
                     /locus_tag="VYN69_001691"
     CDS             912..1388
                     /locus_tag="VYN69_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HEV1533925.1"
                     /translation="MAKEPWQEDIYDQEESRAGRRHRNHGGADRMANRILTILASIFF
                     VIVVVMVIVLIYLSSGGSNRTAALKDFHDSDASVVQISSSSSSQPEQSSELESTSSSS
                     EEAANPEGTIKVLAGEGEAAIAARAGISIAQLEALNPGHMATGSWFANPGDVIKIK"
     gene            1397..2068
                     /locus_tag="VYN69_001692"
     CDS             1397..2068
                     /locus_tag="VYN69_001692"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000849376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEV1533926.1"
                     /translation="MKTIQIAIDGPASSGKSTVAKIIAKDFGFTYLDTGAMYRAATYM
                     ALKNQLGVEEVEALLALLDQHPISFGRSETGDQLVFVGDVDITHPIRENEVTNHVSAI
                     AAIPEVREKLVSLQQEIAQQGGIVMDGRDIGTVVLPQAELKIFLVASVDERAERRYKE
                     NIAKGIETDLETLKKEIAARDYKDSHRETSPLKQAEDAVYLDTTGLNIQEVVEKIKAE
                     AEKRM"
     gene            2199..2537
                     /locus_tag="VYN69_001693"
     CDS             2199..2537
                     /locus_tag="VYN69_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002920966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEV1533927.1"
                     /translation="MNNLPNCPKCNSEYVYEDGALLVCPECAHEWNPAEVAEVEEGLV
                     AIDANGNKLTDGDTVTLIKDLKVKGAPKDLKQGTRVKNIRIVEGDHNIDCKIDGFGAM
                     KLKSEFVRKI"
     gene            2540..3169
                     /locus_tag="VYN69_001694"
     CDS             2540..3169
                     /locus_tag="VYN69_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000033944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Pr6Pr family membrane protein"
                     /protein_id="HEV1533928.1"
                     /translation="MSKHYKLVFYSRIFLFLAAFTGVYLEITRHGGFGMLLYYTVLSN
                     LLVTIFTLYLLKVMSRVGENWQRPSLLRLKGGVTMSIMITCVIYHFLLAPIATNFYTL
                     ENFLCHYIVPIWFLADTLFFDKQGQYKIWDPAVWTILPFLYMMFALFNGLVLKLNIPN
                     AKDNPFPYFFLNVNKGWNVVFKWCLIIFVAYMVAGFIFYFIKQIKRKSS"
     gene            3409..4560
                     /locus_tag="VYN69_001695"
     CDS             3409..4560
                     /locus_tag="VYN69_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000808687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1533929.1"
                     /translation="MKQFLERASILALSLVLITSFSISSALPAMFDYYQGYSKEQIEL
                     LVSLPSFGIMMMLLLNGFLEKIFPERLQISLGLLILSLSGTAPFWYQAYPFVFGTRLL
                     FGLGLGMINAKAISIISERYQGKRRIQMLGLRASAEVVGASLITLAVGQLLAFGWTAI
                     FLAYSAGFLVLPLYLLFVPYGKSKKEVKKRAKEASRLTREMKGLIFTLAIEAAVVVCT
                     NTAITIRIPSLMVERGLGDAQLSSFVLSIMQLIGIVAGVSFSFLISIFKEKLLLWSGI
                     TFGLGQIVIALSSSLWVVVAGSVLAGFAYSVVLTTVFQLVSERIPAKLLNQATSFAVL
                     GCSFGAFTTPFVLGAIGLLTHNGMLVFSILGGWLIVISIFVMYLLQKRA"
     gene            4877..5626
                     /gene="truA"
                     /locus_tag="VYN69_001696"
     CDS             4877..5626
                     /gene="truA"
                     /locus_tag="VYN69_001696"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000199202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEV1533930.1"
                     /translation="MTRYKATISYDGYAFAGFQRQPHARSVQEEIEKTLTRLNKGQTI
                     TVHGAGRTDSGVHALGQVIHFDLPYQMDEEKLRFALDTQSPEDIDVISIELVADDFHC
                     RYAKHSKTYEFILDRGRPKNPMRRHYATHFPYPLDVERMQIAIKKLEGIHDFTGFTAS
                     GTSVEDKVRTITEASLIVDETGQFLTFTFSGNGFLYKQIRNMVGTLLKIGNNRMPVEQ
                     IDLILEKKDRQLAGPTAAPNGLYLKEIRYEE"
     gene            5616..6389
                     /locus_tag="VYN69_001697"
     CDS             5616..6389
                     /locus_tag="VYN69_001697"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEV1533931.1"
                     /translation="MKNNRILALSGNDIFSGGGLSADLATYTLNGLHGFVAVTCLTAL
                     TEKGFEVFPTDDTIFQHELDSLRDVEFGGIKIGLLPTVSVAEKALDFIKQRPGVPVVL
                     DPVLVCKETHDVAVSELCQELIRFFPYVSVITPNLPEAELLSGQEIKTLEDMKTAAQK
                     LHDLGAPAVIIKGGNRLSQDKAVDVFYDGQTFTILENPVIQGQNAGAGCTFASSIASH
                     LVKGDKLLPAVESSKAFVYRAIAQADQYGVRQYEANKNN"
     gene            6367..6828
                     /locus_tag="VYN69_001698"
     CDS             6367..6828
                     /locus_tag="VYN69_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000814967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1533932.1"
                     /translation="MKQTKTTKIALVSLLTALSVVLGYFLKIPTPTGILTLLDAGVFF
                     AAFYFGSREGAVVGGLASFLIDLLSGYPQWMFFSLVNHGLQGFFAGFKGKSQWLGLIL
                     ATIAMVGGYALGSTLMNGWAAALPEILPNFMQNMVGMIVGFILSQSIKKIK"
BASE COUNT         2049 a         1241 c         1549 g         2159 t
ORIGIN      
        1 taattttcct gatatagtta ttttggataa gaattatcaa aataaagcca gaaaaagtat
       61 agaaattgaa gaatggaaga aacttcgagg atgcgtggaa attaagagtt taaagcatga
      121 tttaattaca gaggaaaaaa tcaaatcaac catatcgaac agttttgaac atataactgg
      181 agagatggga caacttattg gagacctttt atggtataaa aaagtaattt atacaaatgg
      241 aattgaatgg agatttctaa gtttagatga caaggaagag atagataata caattgttca
      301 agtagttaac aaaagaatag aaacagaaga agcgggaaat tcttttgatt ggtggaagaa
      361 tattaaagat ttatcgttca attatacgga tatatattta tctaaagatt gtatacaaga
      421 gtgggatgaa tttgtaaaga aagtaaagga aatagaatgg taggagtcta ttctcattga
      481 gaaatttaga ttataagtcg cagtaagatt tatttagaga ctaaaaaaag accactttat
      541 cttatcaaag aaaaaagtga ccttcctgat tttacagaaa aaaataaagt gaaaagacta
      601 taattttaca tggaaatgtg ctaaactaga gggagtggat tatcgccatt gatttaggct
      661 tcctggtttc caatcagggc gcgagttggg caatttttaa cagcctctaa gacatcctga
      721 ctaggagaaa tttctttttc cagttggtca gggccatcgt aaaaacgcac gattccatta
      781 tcgtggtaat caaataaatc agaataagtt tggcaaagcc cacaggcgat acatcgttca
      841 ggtataagtg tgattttcat gttcatattt atattgtaat aagaaaagta aaaaaaaaca
      901 aggagtaagg tatggcaaaa gaaccgtggc aagaagatat ctatgatcaa gaagaatcaa
      961 gagcagggcg tcggcatcga aaccacggag gggctgatag gatggctaat cgtattttga
     1021 cgatcctagc tagtattttc tttgtaattg tggtggtgat ggtcatcgtt ctcatctatc
     1081 tatcatcggg gggaagtaat cgcacagcag ccttaaaaga ctttcatgat tctgatgcaa
     1141 gtgtagtaca aatctcatct tcaagcagtt ctcagcctga gcagagttca gagctagaat
     1201 ctacttctag tagttcagaa gaagctgcta atcctgaagg aacgattaaa gttctcgcag
     1261 gagaagggga agcagctatt gccgctcgtg caggaatctc cattgctcag ttagaggcct
     1321 tgaatcctgg gcacatggct acaggatctt ggtttgctaa tccaggtgat gttataaaaa
     1381 taaaatagga gccgatatga aaacaattca aattgctatt gatggtcctg cttccagcgg
     1441 taagagtacg gtcgcaaaga ttattgctaa ggattttgga ttcacctacc ttgatacagg
     1501 agctatgtat cgtgcagcga cctatatggc tcttaagaac caattaggag ttgaagaagt
     1561 cgaagccctt ctagccttgt tggaccagca tccaatcagc tttggacgtt cagaaactgg
     1621 agaccagctt gtttttgtag gagatgtgga tattacccat cctatccgtg aaaatgaagt
     1681 gaccaatcat gtttctgcta ttgcagcaat tcctgaagtg cgtgagaaac tggtttctct
     1741 ccaacaagaa attgcccagc aaggcgggat tgttatggat ggtcgcgata ttggaactgt
     1801 tgtattgcca caagcagaat tgaaaatttt cctagtagct tctgttgatg agagagcaga
     1861 gcgtcgttac aaggaaaata ttgccaaggg aattgaaaca gaccttgaaa ccctaaaaaa
     1921 ggaaattgct gcgcgtgact acaaggatag tcatcgtgag acttctcctc tcaaacaagc
     1981 agaggatgct gtctaccttg atacaactgg tttgaacatt caagaagtag ttgaaaaaat
     2041 caaagcagaa gctgaaaaaa gaatgtaaat gtagaaaagc cgatagattg atatcggttt
     2101 ttttctaaat ttgtcaaaat gctgatttta gggtataata gttgctgttc gagaaatttt
     2161 gattttcaaa gaatagtgaa tgatgataag gagaaaccat gaacaattta ccaaattgtc
     2221 caaaatgtaa ctcagagtat gtctacgaag acggtgccct actggtttgc ccagagtgtg
     2281 ctcatgagtg gaatcctgct gaagttgcag aagtagaaga aggtcttgtc gctatcgatg
     2341 ccaacggaaa taaattgact gatggtgata cagtaactct catcaaggac ttgaaagtaa
     2401 aaggtgcgcc aaaagatttg aaacaaggga cgcgcgtgaa aaatatccgc atcgtagaag
     2461 gcgaccacaa tatcgactgt aagattgatg gttttggtgc catgaaactt aaatcagagt
     2521 ttgtgaggaa gatttaagca tgtcaaaaca ctataaactt gtattttata gccgtatctt
     2581 cttgtttcta gcggctttta cgggagttta tcttgaaatt actaggcatg gtggttttgg
     2641 gatgcttctc tattacacgg ttctgtccaa cctcttggta actattttta ccctttatct
     2701 tctaaaggtt atgagccgtg taggtgaaaa ttggcaaagg ccaagtcttt tgcgcttaaa
     2761 aggtggggtc accatgagta tcatgattac ctgtgtgatt taccatttcc tcttggcgcc
     2821 cattgcgact aatttctata ccctagaaaa tttcctttgc cactatatcg ttcccatctg
     2881 gtttttagcg gataccctct tttttgacaa acagggtcaa tacaagattt gggatccagc
     2941 agtgtggacc attttaccct ttctgtatat gatgtttgct ctttttaatg gcttggttct
     3001 aaaactcaat attccaaatg ccaaggataa tcctttccct tacttctttt tgaatgtgaa
     3061 caagggttgg aatgttgtgt ttaagtggtg tctgattatc tttgttgcct atatggtagc
     3121 aggatttatt ttctacttta tcaagcaaat caagagaaag tcatcctaat actcttcgaa
     3181 aatctcttca aaccacgtca gtttccatct gcaacctcaa aacactgttt tgaacaacct
     3241 gtggctagct tcctagtttg ctctttgatt ttcattgagt ataagataca agggattcat
     3301 tttcgaattt aagaaggagt ctacaaacca atgaggcttc ttcttttctt gcttatttga
     3361 taactatcaa aaaagtagaa gattaagaga aatatagaaa gagatttcat gaaacaattt
     3421 ttagaacggg ccagcatttt ggctctctcc ctcgttttga ttacctcctt ttccatttcg
     3481 agtgccctac cagccatgtt tgactattat cagggttatt ctaaggaaca aattgagctc
     3541 ttggtgagct tgccttcctt tggaatcatg atgatgttac tgctaaatgg tttcttagaa
     3601 aaaatatttc ctgagcgctt acagattagt ttgggcttgc tgattttatc attgagcggt
     3661 acagctccct tctggtacca agcctatccc tttgtctttg gaacacggct tctctttggt
     3721 ttgggtcttg ggatgatcaa tgccaaggcc atttctatta tcagtgaacg ctaccaagga
     3781 aaaaggcgaa ttcagatgtt agggctacgc gcttctgcag aggtcgttgg agcttctctc
     3841 attaccttgg ccgtcggtca gttgttggcc tttggttgga cagctatctt tctagcctat
     3901 agtgctggat ttttggtgct gcccctttat ctgctctttg tcccttatgg aaaatcaaag
     3961 aaagaagtca agaaaagagc gaaggaagca agtcgtttaa ctcgagaaat gaaaggcttg
     4021 atttttacct tagctatcga agcggcagtt gtagtttgta ccaatacagc tattaccatc
     4081 cgtattccaa gtttgatggt ggaaagagga ttgggggatg cccagttatc tagttttgtt
     4141 cttagtatca tgcagttgat cgggattgtg gctggggtga gtttttcttt cttgatttct
     4201 atctttaaag agaaactgct cctctggtct ggtattacct ttggcttggg gcaaatcgtg
     4261 attgccttgt cttcatcctt gtgggtggta gtagcaggaa gtgttctggc tggatttgcc
     4321 tatagtgtag tcttgacgac ggtctttcaa cttgtctctg aacgaattcc agctaaactc
     4381 ctcaatcaag caacttcatt tgctgtatta ggctgtagtt tcggagcctt tacgacccca
     4441 ttcgttctag gtgcaattgg cttactaact cacaatggga tgttggtctt tagtatctta
     4501 ggaggttggt tgattgtaat ctctatcttt gtcatgtacc tacttcagaa gagagcttag
     4561 gattgattcc taagtttttc ttttgatact caatgaaaat caaagagcaa actatagttg
     4621 attgagtttg gaatagtacg ctgtagctgc taaaatattt ctagaaatta atttgacttt
     4681 cctaatagag ttgttcatat cttatttcaa tctactatag aaagctagcc gcaggttgct
     4741 caaaatagtg tgttgaggtt gcagatggaa actgacgtgg tttgaagaga ttttcaagag
     4801 tataagattt ttatacccat acaatatttg ttttaggtcc ttgtttacag actaatttct
     4861 tgataaaata gaggttatga cgagatataa agcaactatt tcctatgatg gttatgcctt
     4921 tgctggcttt cagcgccagc ctcatgcgcg tagcgttcag gaagaaattg aaaaaacctt
     4981 gaccagatta aataaagggc aaaccattac tgttcacggt gctggtagga cagatagtgg
     5041 ggttcatgcc ctgggacagg tcattcattt tgacctgcct tatcagatgg atgaggagaa
     5101 actccgtttt gccttggata cccagtctcc tgaagatatt gatgtgattt cgattgagct
     5161 tgtggcagat gattttcatt gccgttatgc caaacatagc aagacctatg agtttatttt
     5221 ggatagagga cgtcccaaaa atcctatgcg ccgtcactat gccacccact ttccctaccc
     5281 actcgatgtg gaacgaatgc agattgcaat caaaaagcta gagggaatcc atgattttac
     5341 cggttttaca gcctctggga ctagtgtaga ggataaggtt cgcaccatca cagaagctag
     5401 tttaatagtc gatgagacag gacaattttt gacctttacc ttttcaggaa atggtttctt
     5461 gtataaacag attcgcaata tggtggggac tcttctcaaa atcggtaaca accgcatgcc
     5521 agtagagcag attgatctca tcttggagaa gaaggacagg caacttgcag gtcccactgc
     5581 agcaccaaat ggtttgtatt taaaggagat tcgttatgaa gaataatcgt attttagcac
     5641 tttctggaaa tgatattttt agtggtggtg gactgtcagc tgatttggct acctatacct
     5701 tgaacggctt gcatgggttt gtagcagtga cttgtttgac agccttgaca gaaaaaggat
     5761 ttgaagtctt tccaactgat gataccattt ttcaacatga attagatagc ttgcgtgatg
     5821 tggaatttgg gggaattaag attggtcttc tccctactgt cagcgtggct gagaaggcct
     5881 tggactttat caaacaacgc ccaggagtac ctgtggtgtt ggatcctgtc ttggtctgca
     5941 aggaaacgca tgatgtagct gtcagtgagc tctgccaaga gttgattcgc tttttccctt
     6001 atgtcagtgt gattacgcct aatctcccag aagcagaatt attatccggt caggaaatta
     6061 aaaccttgga agacatgaaa actgcagcgc agaaattgca tgatttagga gcgccagcag
     6121 tcattatcaa gggaggcaat cgtcttagtc aggacaaggc tgtggatgtc ttttatgatg
     6181 gacagacctt tactatccta gaaaatccag ttatccaagg ccaaaatgct ggtgcaggtt
     6241 gtacctttgc ctctagcatt gccagtcacc tggttaaagg tgataaactt ttgccagcag
     6301 tagaaagctc taaggctttc gtttatcgtg ctattgcaca agcagatcag tatggagtaa
     6361 gacaatatga agcaaacaaa aacaactaaa atcgcccttg tatccctatt aaccgccctt
     6421 tctgtggttc taggttattt cttaaaaatc ccaacaccta caggaattct aactctttta
     6481 gatgctggtg tcttctttgc ggccttttac tttggtagtc gtgaaggagc ggtagtcgga
     6541 ggactagcaa gtttcttgat tgacctctta tcaggctacc ctcagtggat gttctttagc
     6601 ttggtcaacc atggcttgca gggatttttc gcaggattta aaggaaaaag tcagtggtta
     6661 ggccttattt tagcaactat tgccatggta ggaggctacg ccttgggttc tactttgatg
     6721 aatggctggg cagcagccct cccagaaatt ctaccaaatt tcatgcaaaa tatggtaggg
     6781 atgattgtag gatttattct tagtcaaagt atcaagaaga ttaagtaata ctcttcgaaa
     6841 atctcttcaa accacgtcag cttccatctg caacctcaaa acactgtttt gagcagcctg
     6901 aggctagctt tctagtttgc actttgattt tcattgagta taaagaggtt gagtaaaaag
     6961 tatttcaaat aagaaaaacg catagtatca ggtgttga
//
LOCUS       DAVLVP010000088         6825 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.088, whole genome shotgun sequence.
ACCESSION   DAVLVP010000088 DAVLVP010000000
VERSION     DAVLVP010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6825)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6825)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6825
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.088"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..2269
                     /locus_tag="VYN69_001699"
     CDS             <1..2269
                     /locus_tag="VYN69_001699"
                     /EC_number="3.4.14.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Xaa-Pro dipeptidyl-peptidase"
                     /protein_id="HEV1533933.1"
                     /translation="FNQYSYINFPKENVLSELKKCGFDLQNTANHKDSLETFLRRFFF
                     TYQDTNYPLSILAADKKTDLLTFFQSEDELTADIFYTVAFQLLGFSYLVDFEDSDVFR
                     KETVFPIIYGDLIENLYQLLNTRTKKGNTLIDQLVSDGLIPEDNDYHYFNGKSLATFS
                     NQDVIREVVYVESRVDTDQKGLSDLVKVSIIRPRFDGKIPAIMTASPYHQGTNDKASD
                     KALYKMEGELEVKLPHKIELEKPQLNLVQPQGKAELIAEAEEKLTHINSSYTLNDYFL
                     PRGFANLYVSGVGTKDSTGFMTNGDYQQIEAYKNVIDWLNGRCRAFTDHTRQRQVKAD
                     WSNGKVATTGLSYLGTMSNGLATTGVDGLEVIIAEAGISSWYNYYRENGLVTSPGGYP
                     GEDFDSLAELTYSRNLLAGDYIRGNEAHQADLEKVKAQLDRKTGDYNQFWHDRNYLLN
                     AHKVKAEVVFTHGSQDWNVKPLHVYQMFHALPTHIHKHLFFHNGAHVYMNNWQSIDFR
                     ESINALLTKKLLGQETDFQLPTVIWQDNTAPQTWLSLDNFGGQENCETFSLGQEEQAI
                     QNQYPDKDFERYGKTYQTFNTELYQGKANQITINLPVTKDLHLNGRAQLNLRIKSSTN
                     KGLLSAQLLEFGQKKYLQPYPAILSARTIDNGRYHMLENLCELPFRPEAQRVVTKGYL
                     NLQNRNDLLLVEDITADEWMDVQFELQPTIYKLKEGDTLRLVLYTTDFEITIRDNTDY
                     HLTVDLAQSMLTLPC"
     gene            2286..2756
                     /locus_tag="VYN69_001700"
     CDS             2286..2756
                     /locus_tag="VYN69_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEV1533934.1"
                     /translation="MNKSEHRHQLIRALITKNKIHTQAELQALLAENDIQVTQATLSR
                     DIKNMNLSKVREEDSAYYVLNNGSISKWEKRLELYMEDALVWMRPVQHQVLLKTLPGL
                     AQSFGSIIDTLSFPDAIATLCGNDVCLIICEDADTAQKCFEELKKFAPPFFFEE"
     gene            complement(3044..3145)
                     /locus_tag="VYN69_001701"
                     /pseudo
     CDS             complement(3044..3145)
                     /locus_tag="VYN69_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001820552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopyranose mutase"
     gene            complement(3389..6739)
                     /locus_tag="VYN69_001702"
     CDS             complement(3389..6739)
                     /locus_tag="VYN69_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000526318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEV1533935.1"
                     /translation="MGNFSFLLKNDEYESFSKPCIEAENMIATSTVATAFMARRALEQ
                     AVHWIYSHDSYLEAPYRATLSSLVWDDDFRDIVDSELHKQIVLLIRWGNHAAHGGEIK
                     EREAILALHHLYQFVNFIDYCYSNEFVERYFDEKCLPLSANIKYRETPQSMIKLQDSL
                     PELPDFHEQMAAQSVEVQETYTEKRETAAQRQDVPFHIEQLSEAETRKLFIDIDLRLA
                     GWIFEENCRVEIAVDGLKHGSGIGYCDYVLYGKNGKILAIVEAKKASVNPEVGEVQVK
                     EYAEALEKHIGYQPICFITNGLKHYILDGPNRRQIAGFYSQEELQLVMDRRHLQKPLE
                     DISSKIRDDISGRHYQKHAIASVCEAFSNHRRQVLLVMATGAGKTRTAVSLVDILSRH
                     NWVKNVLFLADRTSLVKQAYDSFRKLLPDLSVCNFLEDKEGAQSSRMVFSTYPTMIGA
                     ISGQEEVNQRPFTVGHFDLIIIDESHRSIYQKYKSIFDYFDARIVGLTATPRQDLDKN
                     TYGFFNLENGVPTYAYDLEEAVKDGYLVAYHSIETKLKLPTDGLHYDDLSKEEKEHFD
                     SKFEDDSCEKDIDGSVFNSFIFNKSTVEIVLNELMTRGIQTASGDEIGKTIIFAKNHD
                     HAEYIRGIFNNRYPEKGSDYAQVIDYSIKHYQTLIDDFKIKEKYPQIAISVDMLDTGI
                     DVPEVVNLVFFKKVRSKTKFWQMIGRGTRLCKDLFGPEQDKENFLVFDYGDNFDYFRA
                     DPRDGEGRHIVSLTQRLFNIKVDLIRELQGLQYQEDQFARAYRQQLVSELQGRIESLN
                     ELDFRVRMVLDTVYSYRKLESWQNLTAVTSETIQKNLSPLLFDEDKEDEMARRFDLWL
                     LHIQLGQLTAKSSTVHISQVMKTARALSAIGNIPQVFEQAEIIRKVQEPEFWKEVNLS
                     DLEKIRLAIRDLLQFLDKTDRKPYYVNFEDRILSTVHETTAFLQVNDLRSYNEKVEHY
                     LKTHLDEESISKLYHNKKLTSDDMLALEKLLWEKLGSKADYQSHYENKAIPRLVREII
                     GLDRESANRIFSKFLSDENLNARQISFVKLIVDYIVENGFLETKVLTQEPFKSYGSVQ
                     LLFQHQLPVLRNIVQIIELINNLAGEAA"
BASE COUNT         2111 a         1485 c         1153 g         2076 t
ORIGIN      
        1 ttttaatcaa tatagttata tcaattttcc aaaagaaaat gtcttatctg aactaaaaaa
       61 atgtggcttt gatttacaaa atacagccaa tcacaaagac tcacttgaaa cctttttacg
      121 tcgtttcttt ttcacttatc aagacaccaa ctacccactt tctatcctag cagctgataa
      181 aaaaacagac ctgctgacat tttttcaatc agaagatgaa ctgacagcag atatttttta
      241 tactgttgct tttcaacttt taggcttttc ttatttggtt gatttcgaag atagtgacgt
      301 ttttcgtaaa gaaactgtat ttcccattat atatggtgac ttgattgaaa atctctatca
      361 gttactcaat actcgcacca aaaaaggaaa tactcttatc gaccaacttg ttagtgatgg
      421 tcttattccg gaggataatg actaccacta ctttaacggc aagagtttgg ctactttttc
      481 taaccaggat gtcattcgtg aagtcgttta cgttgagtct cgtgtcgata ctgaccaaaa
      541 agggctatca gacctagtaa aggttagcat tattcgccct cgttttgatg gaaaaatccc
      601 tgccatcatg acagccagcc cttatcatca gggaaccaat gacaaggcta gtgacaaggc
      661 tctctacaag atggagggcg agcttgaggt taagcttcct cacaagattg agctagagaa
      721 acctcaacta aatctcgtcc aaccccaagg taaagctgag cttatagcag aggctgagga
      781 aaagctaact cacatcaact ctagctatac actaaacgac tacttccttc cgagaggctt
      841 tgccaatctc tatgtctcag gtgttggtac caaagactcg acaggtttca tgactaatgg
      901 agactaccag caaatcgagg cttataaaaa tgtcatcgat tggcttaacg gccgttgccg
      961 tgcctttact gatcacacgc gccagcgtca agtcaaggct gattggtcaa acggaaaagt
     1021 tgctacaaca ggactttcct atctaggtac catgtccaat ggtcttgcga ctacaggagt
     1081 cgatggttta gaagttatca ttgccgaggc aggaatttca tcatggtaca actactaccg
     1141 tgaaaatggt ctggtgacta gcccaggtgg ttatccaggt gaggactttg actcccttgc
     1201 tgagttaacc tattctcgta atctcttagc tggcgactat atccgtggca atgaagctca
     1261 ccaagctgac ttagaaaaag tgaaagctca gctagaccga aaaactgggg actacaatca
     1321 gttttggcat gaccgcaatt atctgctcaa tgcccataaa gtaaaggcag aggttgtctt
     1381 tactcatggt tctcaggatt ggaatgtcaa accacttcat gtttaccaga tgttccatgc
     1441 tcttcctact catatacaca agcatctctt tttccataat ggtgctcatg tttacatgaa
     1501 caattggcaa tcaattgact tccgtgagtc cataaatgcc ttattgacca agaaattact
     1561 aggacaggaa acagactttc aacttcctac tgttatctgg caggacaata cagctccaca
     1621 gacttggtta tcacttgata acttcggtgg gcaagaaaac tgtgaaacct tctcacttgg
     1681 tcaagaagag caagctattc aaaaccagta cccagataag gattttgagc gctatggtaa
     1741 gacataccaa accttcaata cagagctcta tcaagggaaa gccaatcaga ttactattaa
     1801 ccttcctgtg actaaagatc tccacctaaa cggtcgcgct cagctcaatc ttcgtatcaa
     1861 atccagtaca aacaaggggc tcttatctgc ccaactgcta gaatttgggc aaaagaaata
     1921 cctacaacct tatccagcta ttttaagtgc tagaaccatt gataacggtc gctatcacat
     1981 gttggaaaat ctctgtgaat tgccatttag accagaggca caacgagtcg taacaaaagg
     2041 ttaccttaat ttacaaaata gaaatgattt actgttagta gaggatatta ctgcagatga
     2101 atggatggac gttcaatttg aactgcagcc aactatttac aagctaaaag aaggagacac
     2161 tctccgttta gtcctctata ctactgactt tgaaatcacc atccgtgaca atacagacta
     2221 ccacttgact gtcgacctcg ctcagtccat gcttacctta ccttgctaaa aggagttaca
     2281 ttattatgaa taaatcagaa caccgccacc aacttatacg cgctcttatc acaaaaaaca
     2341 agattcatac acaggctgag ttgcaagccc ttcttgctga gaacgacatt caagtaaccc
     2401 aggcaaccct ctcacgcgac atcaaaaata tgaacctatc aaaagtccgc gaagaagata
     2461 gcgcttatta tgttcttaac aatggttcca tctcaaaatg ggaaaaacgt ctcgaactct
     2521 acatggaaga cgcccttgtc tggatgcgcc cagttcaaca ccaagtccta ctaaaaaccc
     2581 ttcctggact ggctcaatcc tttggttcta tcattgatac tttgagcttc cctgacgcta
     2641 tcgctaccct ttgtggtaat gatgtctgtc ttatcatctg tgaagatgca gatactgctc
     2701 aaaagtgctt tgaagaactg aaaaaattcg ccccaccatt tttctttgaa gaataataaa
     2761 tgactacaac taacaattat gcttttaaaa aacctggtag aaagtcttta acatcagaaa
     2821 acgcatagta tcaagggttg aaaacccttg atactatgcg ttttgcttta tcctaggaag
     2881 atttatactc ttcgaaaatc tcttcaatcc gcgtcaacgt cgccttgccg taggtatggt
     2941 tactgacttc gtcagtttca tctacaacct caaaaccatg ttttgagctg acttcgtcag
     3001 tttcatctac aacctcaaaa ccatgttttg agctgacttc gtcagtctta tctacaacct
     3061 caaaacagtg ttttgagcag cctgcagcta gtttcctagt ttgctctttg attttcattg
     3121 agtattattt cattttctcc tgcaattgaa ttcttgctcg gctttttgtt ttctatttct
     3181 ttaaaatcaa agtagctctt ttgttaataa ctcgatcaac aaacatcgtg gtacaagtat
     3241 ctactttgaa atttatcaac cacttaacaa ctgatactgt attctaggaa aacgatgaca
     3301 ttcttcctaa taaaacttct catatatagc ataaatttct actcttttta attttaaatg
     3361 agtctcagca tggcaatcac tttagaattt aagccgcttc tccagctaga ttattgataa
     3421 gttcaatgat ttgaacaata ttacgaagta ctggtagttg gtgttggaag agtagttgaa
     3481 cagatccata agatttaaac ggctcttgcg ttaacacttt cgtctctaaa aaaccatttt
     3541 ctacaatgta gtctacaatc aattttacaa atgaaatctg cctggcatta agattctcat
     3601 ccgacaaaaa tttagaaaaa atacgattgg cagactctct atctaagcca ataatctcac
     3661 gaaccaatct cggaattgcc ttattttcat aatgactttg gtagtctgct ttactaccta
     3721 atttttccca aagcaatttt tcaagtgcaa gcatatcatc agatgtcaac tttttattat
     3781 ggtatagctt agaaatggac tcctcatcca gatgagtttt caaataatgc tcaacttttt
     3841 cattgtaaga ccgaagatcg ttgacctgca aaaatgctgt ggtctcgtga acagtggaga
     3901 gtatacgatc ttcaaagtta acatagtagg gtttacggtc tgttttatcc aaaaactgta
     3961 ataaatctcg aatagcaaga cgaatttttt ccaaatcaga caagttaact tctttccaaa
     4021 attcaggctc ctgtactttc ctgataattt cagcctgctc aaaaacctgc gggatattgc
     4081 caatagcaga aagagctcta gccgtcttca tcacttggga aatatgaaca gtggaagatt
     4141 tagctgtcag ttgccccaac tgaatatgaa gcaaccacaa atcaaatctc ctggccatct
     4201 catcttcttt atcttcatca aataaaagcg gagagagatt tttttgaatg gtttcacttg
     4261 taacagcagt tagattctgc caactttcca atttcctata gctataaact gtatctaaaa
     4321 ccatacgaac cctgaagtcc aactcattta agctctctat acgaccttga agttccgaga
     4381 caagctgctg acggtatgct ctcgcaaact gatcttcttg gtattggagt ccctgaagtt
     4441 ctcgaatcaa gtccactttg atattaaata aacgctgagt caacgaaaca atgtgacgac
     4501 cctctccatc tcttggatct gcacgaaaat aatcaaaatt gtccccataa tcaaatacca
     4561 agaagttttc cttatcctgc tcaggtccaa ataaatcttt acatagacgg gttcctcgac
     4621 caatcatctg ccaaaactta gttttagagc gtactttctt gaagaagact aaattaacaa
     4681 cctctggtac atcaatacct gtatctaaca tatcgacaga aatcgcaatt tgaggatact
     4741 tctccttaat tttaaaatca tcaatcaagg tctgataatg cttaatacta taatcaatca
     4801 cctgagcata gtcgctccct ttttcaggat agcggttgtt aaaaatacct ctgatatatt
     4861 ccgcatggtc atgattttta gcaaaaataa tagttttacc aatttcatca cccgaggctg
     4921 tctgaattcc tcttgtcatg agttcattta aaacaatttc tactgtactt ttattgaaaa
     4981 taaaggaatt aaatacactc ccatcaatat ctttttcaca gctatcgtct tcaaatttgc
     5041 tatcaaaatg ttccttttct tctttggaca aatcatcata atgtagacca tccgtaggta
     5101 gtttcagttt ggtttcgata gaatgatagg ctactaaata tccgtcttta acagcctctt
     5161 ccaaatcata tgcatatgtt ggaaccccat tctccaaatt aaagaatcca taggtgtttt
     5221 tatctaaatc ttgacgcgga gtagctgtta agcctacaat tcttgcatca aaataatcaa
     5281 aaatggactt gtatttctga taaatagaac ggtgagattc gtcaattatg ataaggtcaa
     5341 aatgcccaac agtaaaaggg cgttgattta cttcttcttg accactaatc gctccaatca
     5401 tggtcggata agttgaaaag accatacgac ttgattgagc tccttcttta tcttctaaga
     5461 agttacaaac ggaaagatct gggagtaatt ttctaaacga atcataggct tgcttaacca
     5521 aggaagttct atcggctaag aagagaacgt tttttaccca gttatgacgt gataagatat
     5581 caactagaga aactgctgta cgagttttcc ccgccccagt tgccataacc aaaagtacct
     5641 gtctacgatg attagagaaa gcttcacaaa cgcttgcaat ggcatgtttt tggtagtgac
     5701 gcccggaaat atcgtcccta attttactag aaatatcctc aagcggtttt tgaagatgac
     5761 gtctatccat cactaattgc aattcttctt gagagtaaaa gcctgcaatc tggcggcggt
     5821 tcggaccatc aagtatatag tgcttcaacc catttgtaat aaagcaaatt ggctgatagc
     5881 cgatatgttt ctccaaagct tcagcatatt ctttgacctg tacttcccct acttctggat
     5941 tgacagaggc ttttttagcc tccacaatcg ctaaaatttt cccattttta ccataaagta
     6001 cgtagtcaca gtaaccaatt cctgaaccgt gcttgagacc atcaacggct atctcaacac
     6061 gacagttttc ttcaaatatc catcctgcta aacggagatc gatatcaata aagagctttc
     6121 ttgtctctgc ctcagataat tgttcaatat ggaaaggcac atcttgccgt tgcgctgcag
     6181 tctcacgttt ttcagtataa gtctcttgaa cttctacgga ctgagcagcc atctgttcat
     6241 gaaaatcagg cagttctggt aaactgtctt gtaactttat catagattgt ggagtttctc
     6301 ggtatttgat gtttgctgaa agtggtaagc acttctcatc aaaataacgc tccacaaatt
     6361 cattgctgta acaataatcg ataaaattaa caaactgata caaatgatgt aaagctaaaa
     6421 tcgcttctcg ttccttaatt tcaccaccat gagcagcatg gtttccccac cgaatcaaca
     6481 gaactatctg cttgtggagt tcagaatcta cgatatccct aaaatcatca tcccatacta
     6541 aagaagatag agtagcacga tagggagctt ctaaatatga atcgtgacta tatatccaat
     6601 ggacagcctg ctccaaagca cgacgcgcca taaaggcagt agccacagtt gatgtagcaa
     6661 tcatattttc agcttcaatg caaggttttg aaaaagattc atattcgtca ttttttaaaa
     6721 gaaagctaaa atttcccatt tttttctcac tttaaacaaa tagtttagct atattatact
     6781 aaatttcaag ctatttctcc acacttttat agctatctca caagc
//
LOCUS       DAVLVP010000089         6757 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.089, whole genome shotgun sequence.
ACCESSION   DAVLVP010000089 DAVLVP010000000
VERSION     DAVLVP010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6757
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.089"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            73..1350
                     /locus_tag="VYN69_001703"
     CDS             73..1350
                     /locus_tag="VYN69_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001068247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1533936.1"
                     /translation="MNPIQRSWAYVSRKRLRSFILFLILLVLLAGISACLTLMKSNKT
                     VESNLYKSLNTSFSIKKIENGQTFKLSDLASVSKIKGLENVSPELETVAKLKDKEAVT
                     GEQSVERDDLSAADNNLVSLTALEDSSKDVTFTSSAFNLKEGRHLQKGDSKKILIHEE
                     LAKKNGLSLHDKIGLDAGQSESGKGQTVEFEIIGIFSGKKQEKFTGLSSDFSENQVFT
                     DYESSQTLLGNSEAQVSAARFYVENPKEMDGLMKQVENLALENQGYQVEKENKAFEQI
                     KDSVATFQTFLTIFLYGMLIAGAGALILVLSLWLRERVYEVGILLALGKGKSSIFLQF
                     CLEVVLVSLGALLPAFVAGNAITTYLLQTLLASGDQASLQDTLAKASSLSTSILSFAE
                     SYVFLVLLSCLSVALCFLFLFRKSPKEILSSIS"
     gene            1363..2010
                     /gene="vex2"
                     /locus_tag="VYN69_001704"
     CDS             1363..2010
                     /gene="vex2"
                     /locus_tag="VYN69_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000173730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding subunit Vex2"
                     /protein_id="HEV1533937.1"
                     /translation="MTLLQLQDVTYRYKNTAEAVLYQINYNFEPGKFYSIIGESGAGK
                     STLLSLLAGLDSPVEGSILFQGEDIRKKGYSYHRMHHISLVFQNYNLIDYLSPLENIQ
                     LVNKKASKDTLLELGLDESQIKRNVLQLSGGQQQRVAIARSLVSEAPVILADEPTGNL
                     DPKTAGDIVELLKSLAQKTGKCVIVVTHSKEVAQASDITLELKDKKLTETRNTSK"
     gene            2062..3441
                     /gene="vex3"
                     /locus_tag="VYN69_001705"
     CDS             2062..3441
                     /gene="vex3"
                     /locus_tag="VYN69_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004269839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit Vex3"
                     /protein_id="HEV1533938.1"
                     /translation="MLHNAFAYVTRKFFKSIVIFLIILLMASLSLVGLSIKGATAKAS
                     QETFKNITNSFSMQINRRVNQGTPRGAGNIKGEDIKKITENKAIESYVKRINAIGDLT
                     GYDLIETPETKKNLTADRAKRFGSSLMITGVNDSSKEDKFVSGSYKLVEGEHLTNDDK
                     DKILLHKDLAAKHGWKVGDKVKLDSNIYDADNEKGAKETVEVTIKGLFDGHNKSAVTY
                     SQELYENTAITDIHTAAKLYGYTEDTAIYGDATFFVTADKNLDDVMKELNGISGINWK
                     SYTLVKSSSNYPALEQSISGMYKMANLLFWGSLSFSVLLLALLLSLWINARRKEVGIL
                     LSIGLKQASILGQFITESILIAIPALVSAYFLANYTARAIGNTVLANVTSGVAKQASK
                     AAQASNLGGGAEVDGFSKTLSSLDISIQTSDFIIIFVLALVLVVLVMALASSNLLRKQ
                     PKELLLDGE"
     gene            3621..3704
                     /gene="pep27"
                     /locus_tag="VYN69_001706"
     CDS             3621..3704
                     /gene="pep27"
                     /locus_tag="VYN69_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_198523890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative autolysis signal peptide Pep27"
                     /protein_id="HEV1533939.1"
                     /translation="MRKEFHNVLSSDQLLTDKRPARDYNRK"
     gene            3753..4409
                     /gene="vncR"
                     /locus_tag="VYN69_001707"
     CDS             3753..4409
                     /gene="vncR"
                     /locus_tag="VYN69_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000697958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor VncR"
                     /protein_id="HEV1533940.1"
                     /translation="MKILIVEDEEMIREGVSDYLTDCGYETIEAADGQEALEQFSSYE
                     VALVLLDIQMPKLNGLEVLAEIRKTSQVPVLMLTAFQDEEYKMSAFASLADGYLEKPF
                     SLSLLKVRVDAIFKRYYDTGRIFSYKDTKVDFESYSASLAGQEVPINAKELEILDYLV
                     KNEGRALTRSQIIDAVWKATDEVPFDRVIDVYIKELRKKLDLDCILTVRNVGYKLERK
                     "
     gene            4406..5734
                     /gene="vncS"
                     /locus_tag="VYN69_001708"
     CDS             4406..5734
                     /gene="vncS"
                     /locus_tag="VYN69_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase VncS"
                     /protein_id="HEV1533941.1"
                     /translation="MKRTGLFAKIFIYTFSIFSVLVICLHLAIYFLFPSTYLSHRQET
                     IGQKATAIAQSLEGKDRQSIEQVLDLYSQTSDIKGTVKGEMTEDKLEVKDSLPLDTDR
                     QTTSLFIEEREVKTQDGGTMILQFLASMDLQKEAEQISLQFLPYTLLASFLISLLVAY
                     IYARTIVAPILEIKRVTRRMMDLDSQVRLRVDSKDEIGNLKEQINSLYQHLLTVIADL
                     HEKNEAILQLEKMKVEFLRGASHELKTPLASLKILIENMRENIGRYKDRDQYLGVALG
                     IVDELNHHVLQILSLSSVQELRDDRETIDLLQMTQNLVKDYALLAKERELQIDNSLTH
                     QQAYLNPSVMKLILSNLISNAIKHSVPGGLVRIGEREGELFIENSCSSEEQEKLAQSF
                     SDNASRKVKGSGMGLFVVKSLLEHEKLAYRFEMEENRLTFFIDFPKVVQD"
     gene            5875..6756
                     /locus_tag="VYN69_001709"
     CDS             5875..6756
                     /locus_tag="VYN69_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001018994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEV1533942.1"
                     /translation="MAIVSAEKFVQAARDNGYAVGGFNTNNLEWTQAILRAAEAKKAP
                     VLIQTSMGAAKYMGGYKVARNLIANLVESMGITVPVAIHLDHGHYEDALECIEVGYTS
                     IMFDGSHLPVEENLKLAKEVVEKAHAKGISVEAEVGTIGGEEDGIIGKGELAPIEDAK
                     AMVETGIDFLAAGIGNIHGPYPVNWEGLDLDHLQKLTEALPGFPIVLHGGSGIPDEQI
                     QAAIKLGVAKVNVNTECQIAFANATRKFARDYEANEAEYDKKKLFDPRKFLADGVKAI
                     QASVEERIDVFGSEGKA"
BASE COUNT         2073 a         1325 c         1483 g         1876 t
ORIGIN      
        1 tttttttttt cgtatttcat aaacatttca tatttgggtt ttataatagt cttacaaata
       61 tggaggtgac aaatgaatcc aatccaaaga tcttgggctt atgtcagcag aaagcgactg
      121 agaagtttta ttttatttct gattttattg gtcttattgg ccggaatttc agcctgtttg
      181 actctgatga agtccaacaa aacagtagaa agcaatctgt ataaatcact caatacatct
      241 ttttctatta agaagataga gaatggtcag acattcaagt tgtcagacct agcatctgta
      301 agcaagatta aggggctgga aaatgtctct cctgaacttg agacggtcgc aaaactaaaa
      361 gacaaggaag cagtgactgg cgagcagagc gtggagcgtg atgatttatc agctgcagac
      421 aataacttgg ttagcttaac ggctcttgag gattcatcca aggatgtaac ctttaccagt
      481 tcggctttca atctaaaaga agggcgacac cttcaaaaag gggattccaa gaaaatcctt
      541 atccacgaag aattggctaa gaagaacggt ctttcacttc atgacaagat tggcttggat
      601 gctggtcagt ctgaatctgg aaaaggacaa acagtagagt ttgagattat tggcatcttt
      661 tctggtaaaa aacaagagaa attcacaggc ttgtcttctg acttcagtga aaatcaagtc
      721 tttacagact atgaaagtag ccaaaccctt ttgggcaata gtgaagctca agtcagtgca
      781 gcacgcttct atgtagaaaa tcctaaggaa atggacggac tcatgaagca ggtagaaaac
      841 ttggccttgg aaaatcaagg ctaccaagtc gaaaaggaaa acaaggcttt tgaacaaatc
      901 aaagactcag ttgcaacttt ccaaaccttc ctgaccatct tcctttatgg gatgttgata
      961 gcaggagctg gagccttaat tctggttttg tctctctggt tgagagaacg ggtctatgaa
     1021 gtggggattt tacttgcact tggaaaaggc aagagctcga tcttcctaca attctgttta
     1081 gaggtagttt tggtatctct tggagctttg cttccagcat ttgttgcagg aaacgcaatc
     1141 acaacttacc tactccaaac tctactagca agtggagatc aggcaagctt acaagataca
     1201 ctagccaaag caagcagttt atcaactagc atcttatctt ttgcagaatc ctatgttttt
     1261 ctagttctgc ttagttgctt atctgtagcc ctttgtttcc tattcttatt tagaaaatca
     1321 ccgaaagaaa ttttatcatc tattagttaa gaaggagaaa tcatgacttt attacaatta
     1381 caagatgtta cctaccgtta taagaatact gctgaagcag tcctatatca gatcaattat
     1441 aattttgaac ccggaaaatt ttacagtatt attggggagt caggagcagg aaaatccaca
     1501 ctcttgtccc tacttgctgg tctagatagt cctgttgaag gttctatcct ttttcaagga
     1561 gaggatattc gtaagaaggg ctattcttac catcgcatgc accatatttc cctggtcttt
     1621 caaaattata acttgataga ttatctttct ccgctggaaa atatccaatt ggtcaacaaa
     1681 aaggcaagca aggatacact tcttgagctt ggtttggatg aaagtcagat caagcggaat
     1741 gttctccagt tatcaggtgg tcaacagcaa cgtgttgcca ttgctcgcag tttggtctca
     1801 gaagctccag ttattctagc tgatgagcca acaggaaatc tggatcctaa aactgctgga
     1861 gatattgtcg aactactcaa atcacttgcc cagaaaacag gtaaatgtgt gattgtcgta
     1921 actcacagta aagaagtggc acaagcgtca gatattacac ttgaattaaa ggataagaaa
     1981 ctgactgaaa cgcgcaatac tagtaaataa tttgagctta ctttaataga atgattaaaa
     2041 caaaatctag aaagggaatc tatgttacac aacgcatttg cctatgttac aaggaagttt
     2101 ttcaaatcga ttgtcatctt cctgattatt ctcctcatgg cgagcttgag tttggtcggc
     2161 ttgtcaatca agggagctac tgccaaggct tctcaggaga cctttaaaaa tatcaccaat
     2221 agcttctcca tgcaaatcaa tcgtcgcgtc aaccaaggaa cgcctcgcgg tgctgggaat
     2281 atcaagggtg aagacatcaa aaaaatcacc gaaaacaagg ccattgagtc ttatgtcaaa
     2341 cgtatcaacg ctatcggaga tttgactgga tatgacctga ttgaaacgcc agaaaccaag
     2401 aagaatctca ctgctgatcg tgccaaacgt tttggaagta gcttgatgat tacaggtgtc
     2461 aatgactcct ctaaagaaga caagtttgtc tctggttctt ataaactagt cgaaggagag
     2521 cacttaacca acgacgacaa ggataaaatc ctcttgcaca aggacttggc agccaaacac
     2581 ggctggaaag taggggacaa ggttaaactg gactctaata tctacgatgc agataatgaa
     2641 aaaggagcca aggaaacagt tgaagtgaca atcaagggac tctttgatgg tcataataag
     2701 tcagcagtaa cctactcaca agaactttac gaaaacacag ctattacaga cattcacact
     2761 gctgcaaaac tttatggata cacagaagac acagccattt atggggacgc aaccttcttt
     2821 gtaacagcag acaagaactt ggatgatgtt atgaaagagt tgaatggcat cagtggtatc
     2881 aactggaaga gctacacact cgtcaagagc tcctctaact acccagctct tgagcaatct
     2941 atctctggta tgtacaagat ggccaacctc ctcttctggg gtagcttgag cttctcagtt
     3001 ctcctccttg ccctcttgct cagcctttgg atcaacgccc gtcgcaagga agtgggaatt
     3061 ctcctctcta tcggcctcaa gcaggcaagt atcttgggtc aattcatcac cgaatctatc
     3121 ttgattgcta tccctgctct agtttctgct tacttcctag ctaattacac tgcccgtgca
     3181 attggaaaca ctgtccttgc caatgtgact tcaggtgttg ccaaacaggc tagtaaggcg
     3241 gctcaagcct ctaaccttgg tggtggtgca gaagtagatg gctttagcaa gaccttgtcg
     3301 agcctagaca tttctattca gacatcagac tttatcatca tttttgtcct tgccttggtt
     3361 ctagtggttc tcgttatggc gcttgcttca agcaatctcc ttagaaaaca accaaaagag
     3421 ctcttgctgg atggtgaata aatttgaaaa aatgagtctg gaataaagat tgcatcttgt
     3481 gtttctattc aagaatagtg gataggaatg gctatttaac aattcaaaat aaatccgaaa
     3541 gcagtggtga aaatcattgc tttcagttgt tttctttgta ctttattgct taaatataat
     3601 atactaaagc tatggaattt atgagaaagg aatttcacaa cgttttatct agtgatcagt
     3661 tacttacaga caaaaggcca gcaagagact ataatagaaa atagagtagg tatttattct
     3721 aagaaaaata aaaaatagag agcagttaaa gtatgaaaat tttaattgta gaagatgaag
     3781 agatgatccg tgagggggtc agtgattatt tgacggattg tggctatgaa actattgagg
     3841 cagcggacgg tcaggaagct ctggagcaat tttctagcta tgaggtggcc ctggttttac
     3901 tggatatcca gatgcccaag cttaacggct tagaagttct agctgagatt cgtaaaacca
     3961 gtcaggttcc tgtcttgatg ttgacagctt ttcaggatga ggaatacaag atgagtgcct
     4021 ttgcctcttt ggcagatggc tatctggaaa aacctttctc cctctccctc ttaaaagtga
     4081 gggtggacgc gattttcaag cgctactacg atacaggacg aatcttttct tacaaggata
     4141 ccaaggtgga ctttgaaagc tacagtgcaa gcctcgcagg tcaagaagtg cctatcaatg
     4201 ccaaagagtt ggaaattctg gactatctag tgaaaaatga aggccgggcc ttgactcggt
     4261 ctcagattat cgatgccgtc tggaaagcga cagatgaggt tccctttgac cgtgttattg
     4321 atgtttatat caaggaattg cggaaaaagc tagacttgga ttgtatcctc actgtgcgca
     4381 atgttggtta taaattggag cgaaaatgaa acgaacaggt ttatttgcaa agatatttat
     4441 ctataccttc tcgatattta gtgttctggt tatctgcctt catttagcta tttattttct
     4501 ttttccttcg acttatctga gtcatcgtca ggaaaccatt ggtcaaaagg caacagccat
     4561 tgcccagtcc ctagaaggga aagataggca gagtatcgag caagtgttag acttgtattc
     4621 ccagactagt gatatcaagg ggaccgtcaa aggtgagatg accgaggaca agttagaagt
     4681 caaggacagt cttcctctgg acacagaccg ccagacaacc tctctcttta ttgaggagcg
     4741 cgaggtgaaa acgcaagacg gtggtactat gattctccag tttctagctt ccatggattt
     4801 acaaaaggaa gcggagcaaa tcagtctcca gtttcttccc tataccttgc tggcctcctt
     4861 tctgatttcc ctcttggtgg cctacatcta cgctcggact attgttgcac cgattttgga
     4921 aatcaagcgg gtgacccgtc ggatgatgga cctggattcc caagtgcgat tgcgcgtgga
     4981 ttctaaggat gagataggta atctcaagga acaaatcaat agcctctacc agcatctctt
     5041 gactgttatt gcggacttgc atgaaaagaa tgaagccatt ctccagctgg agaagatgaa
     5101 ggtcgaattc ctacgaggag cttctcatga attgaaaaca ccgctggcta gtttgaaaat
     5161 cctaatcgaa aatatgagag agaatatcgg tcgttataag gatagagacc agtatctggg
     5221 agttgccttg gggattgtgg atgaactcaa tcaccatgtt ctgcagatac tttccctctc
     5281 ttctgtgcag gaattgcgag atgataggga aacaattgac ctcctccaga tgacgcaaaa
     5341 tctggtcaaa gattatgcct tgctagccaa ggaaagagag ctccagatag acaatagttt
     5401 gacccatcag caggcttatc taaacccatc agttatgaag ttgattcttt ctaatctcat
     5461 cagcaatgcc attaagcact ctgttccagg tggcttagtt cgaattggag aaagagaagg
     5521 agaacttttt atcgaaaata gctgtagctc agaggaacaa gaaaaactag cccagtcttt
     5581 ttctgacaat gctagtcgca aggtcaaggg gtctggtatg gggctctttg tggttaagag
     5641 tctattagaa catgaaaaat tagcttatcg tttcgagatg gaggagaatc gtttaacctt
     5701 ctttatagat tttccaaaag tcgtccaaga ctagggagag aaagggttta cataggtgga
     5761 gttagaagaa aatcaatcga aactgcggga aaaactagat ttttttggca aaaagtgata
     5821 aaatgaacaa tgtaaatggg atgacccata aaaatataca ggaggcctga taaaatggca
     5881 atcgtttcag cagaaaaatt tgtccaagca gcccgtgaca acggttatgc agttggtgga
     5941 tttaacacaa acaaccttga gtggactcaa gctatcttgc gcgcagcaga agctaaaaaa
     6001 gctccagttt tgatccaaac ttcaatgggt gctgctaaat acatgggtgg ttacaaagtt
     6061 gctcgcaact tgatcgctaa ccttgttgaa tcaatgggta tcactgtacc agtagctatc
     6121 caccttgacc acggtcacta cgaagatgca cttgagtgta tcgaagttgg ttatacttca
     6181 atcatgtttg acggttcaca ccttccagtt gaagaaaacc ttaaattggc taaagaagtt
     6241 gttgaaaaag cacacgctaa aggtatctca gtagaagctg aagttggtac tatcggtggt
     6301 gaagaagacg gaatcatcgg taaaggtgaa ttggctccaa tcgaagacgc taaagcaatg
     6361 gttgaaactg gtatcgactt cttggcagct ggtatcggta acatccacgg cccttaccca
     6421 gtaaactggg aaggtcttga ccttgaccac ttgcaaaaat tgacagaagc tcttccagga
     6481 ttcccaatcg tattgcacgg tggatcaggt attcctgatg agcaaatcca agcagctatc
     6541 aaacttggtg ttgccaaagt taacgttaac acagaatgcc aaatcgcatt cgctaacgca
     6601 actcgtaaat ttgctcgtga ctacgaagca aacgaagcag aatacgacaa gaaaaaactc
     6661 ttcgacccac gtaaattctt ggctgacggt gtaaaagcta tccaagcatc ggttgaagaa
     6721 cgtatcgacg tattcggttc agaaggtaaa gcataat
//
LOCUS       DAVLVP010000090         6677 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.090, whole genome shotgun sequence.
ACCESSION   DAVLVP010000090 DAVLVP010000000
VERSION     DAVLVP010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6677)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6677)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6677
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.090"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            94..1056
                     /locus_tag="VYN69_001710"
                     /pseudo
     CDS             94..1056
                     /locus_tag="VYN69_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862469.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
     gene            1233..2240
                     /gene="gap"
                     /locus_tag="VYN69_001711"
     CDS             1233..2240
                     /gene="gap"
                     /locus_tag="VYN69_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008090210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEV1533943.1"
                     /translation="MVVKVGINGFGRIGRLAFRRIQNVEGVEVTRINDLTDPVMLAHL
                     LKYDTTQGRFDGTVEVKEGGFEVNGKFIKVSAERDPEQIDWATDGVEIVLEATGFFAK
                     KEAAEKHLKGGAKKVVITAPGGNDVKTVVFNTNHDVLDGTETVISGASCTTNCLAPMA
                     KALQDNFGVVEGLMTTIHAYTGDQMILDGPHRGGDLRRARAGAANIVPNSTGAAKAIG
                     LVIPELNGKLDGSAQRVPTPTGSVTELVAVLEKNVTVDEVNAAMKAASNESYGYTEDP
                     IVSSDIVGMSYGSLFDATQTKVLDVDGKQLVKVVSWYDNEMSYTAQLVRTLEYFAKIA
                     K"
     gene            complement(2307..3143)
                     /locus_tag="VYN69_001712"
     CDS             complement(2307..3143)
                     /locus_tag="VYN69_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEV1533944.1"
                     /translation="MTVKQLLEEQLLIPRKIRHFLRIKKHILINQEEVHWNEIVNPGD
                     VCQLTFDEEDYSQKTIPWGNPDLVQEVYQDQHLIIVNKPEGMKTHGNQPNEIALLNHV
                     STYVGQTCYVVHRLDMETSGLVLFAKNPFILPILNRLLEKKEISREYWALVDGNINRK
                     ELVFRDKIGRDRHDRRKRIVDAKNGQYAETHVSRLKQFSNKTSLARCKLKTGRTHQIR
                     VHLSHHNLPILGDPLYNSKSKTSRLMLHAFRLSFTHPLTLEKLTFTTLSNTFEKELKK
                     NG"
     gene            3269..5464
                     /locus_tag="VYN69_001713"
     CDS             3269..5464
                     /locus_tag="VYN69_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000762602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEV1533945.1"
                     /translation="MKLDKLFEKFLSLFKKETSELEDSDSTILRRSRSDRKKLAQVGP
                     IRKFWRRYHLTKIILILGLSAGLLVGIYLFAVAKSTNVNDLQNALKTRTLIFDREEKE
                     AGALSGQKGTYVELTDISKNLQNAVIATEDRSFYKNDGINYGRFFLAIVTAGRSGGGS
                     TITQQLAKNAYLSQDQTVERKAKEFFLALELSKKYSKEQILTMYLNNAYFGNGVWGVE
                     DASKKYFGVSASEVSLDQAATLAGMLKGPELYNPLNSVEDSTNRRDTVLQNMVAAGYI
                     DKNQETEAAEVDMTSQLHDKYEGKISDYRYPSYFDAVVNEAVSKYNLTEEEIVNNGYR
                     IYTELDQNYQANMQIVYENISLFPRAEDGTFAQSGSVALEPKTGGVRGVVGQVADNDK
                     TGFRNFNYATQSKRSPGSTIKPLVVYTPAVEAGWALNKQLDNHTMQYDSYKVDNYAGI
                     KTSREVPMYQALAESLNLPAVVTVNDLGVDKAFEAGEKFGLNMEKVDRVLGVALGSGV
                     ETNPLQMAQAYAAFANEGLMPEAHFISRIENASGQVIASHKNSQKRVIDKSVADKMTS
                     MMLGTFTNGTGISSSPADYIMAGKTGTTEAVFNPEYTSDQWVIGYTPDVVISHWLGFP
                     TTDENHYLAGSTSNGAAHVFRNIANTILPYTSGSTFTVENAYKQNGIAPANTKRQVQT
                     NDNSQTDDNLSDIRGRAQSLVDEASRAISDAKIKEKAQTIWDSIVNLFR"
     gene            5517..5669
                     /gene="rpmG"
                     /locus_tag="VYN69_001714"
     CDS             5517..5669
                     /gene="rpmG"
                     /locus_tag="VYN69_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015912025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEV1533946.1"
                     /translation="MALKKASLACAVCGSRNYSIKISGNPKPTRLEVNKFCKHCGKYT
                     THRETR"
     gene            5679..5855
                     /gene="secE"
                     /locus_tag="VYN69_001715"
     CDS             5679..5855
                     /gene="secE"
                     /locus_tag="VYN69_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001211016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEV1533947.1"
                     /translation="MRFIGDIFRLLKDTTWPTRKESWRDFRSIMEYTAFFVVIIYIFD
                     QLIVSGLIRFINIF"
     gene            5910..6446
                     /gene="nusG"
                     /locus_tag="VYN69_001716"
     CDS             5910..6446
                     /gene="nusG"
                     /locus_tag="VYN69_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000376740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEV1533948.1"
                     /translation="MDSFDKGWFVLQTYSGYENKVKENLLQRAQTYNMLDNILRVEIP
                     TQTVQVEKNGKRKEVEENRFPGYVLVEMVMTDEAWFVVRNTPNVTGFVGSHGNRSKPT
                     PLLEQEIRDILVSMGQTFQEFDFDVEIGQTVRIIDGAFADYTGKITEIDNNKVKMIIS
                     MFGNDTVAEVNLNQIAEL"
BASE COUNT         2074 a         1150 c         1465 g         1988 t
ORIGIN      
        1 gaggctcttt tgtcctgctc ttgtttcaat tgactatagt tcacggatac agttgggaaa
       61 gaagctatta aacatttgga gagtcctttt gggatgaaca tgtcaaatat tactatagat
      121 gggcaagaaa tggatctgag tcaaacgatg ccttcttatg ctcaagtagt gagtctttat
      181 gaggatacaa gcatctctgt tagtagtaat gagacggaca aagtgttggc tggatccctc
      241 tatactgata caaatgaaca gggattaacg attccaagca gtttactaaa aaattggaat
      301 gaacagacag gaaaaaattt gacagctaat gatcttattg gcaaatcagt ctcagccagc
      361 attgtagaaa gtgctgccga aactagtaag attgctcaat ttcaaacgaa gattgtacgt
      421 gtaatcaatg atgaagatga catggaggcc agcaacagtt tcatgctgtc tcatcaaatg
      481 gaaacgattt tgaaagaggc tggatttacg aaagctgtat cttattttat cttggaactc
      541 aaagatccat cacagacaaa agtagtaaca gaagaattac agaaaaataa gaagtatact
      601 gtgctttctc aacagagagt tcttgatatt gtgattacct ttattcgtgt tattcaggga
      661 ttattgattg tgctttcatc acaagctatt gtggtagcag cggttatgat tggtatcatt
      721 atttacatca atatcatgta acgttccaag gaaataggtg tcatgaaagc agttggttat
      781 cagaatcgtg atgtcaaagg aatttttatt tacgaggcta tctggattgt aggcatcgcc
      841 ttgctgctgg catttttggt tgcacaaggg gtgggaagtt tggcgaatgc gattgtaagt
      901 cacttttacc catccatcac taaggttttt gaattaaatc ttttatctgt tttaggaact
      961 ctagttttcg ctctattact tggttatgtc tcagcctact tcccggcgcg taagattagt
     1021 aaaatggatc ctgtagaacc gttacgctat gaatagtaag ggattccttg atttacagtc
     1081 ttaaaaagaa actaacatag aaaaacaatg gccatacttg caattcaact caaatagttg
     1141 tataataggt ttgttgaaag gtgatttgta gtgattcaag ttactctttt cacaataaaa
     1201 atttcatgat tttcataagg aggaaatcac taatggtagt taaagttggt attaacggtt
     1261 tcggacgtat cggtcgtctt gctttccgtc gtatccaaaa cgtagaaggt gttgaagtta
     1321 cacgcatcaa cgaccttaca gatccagtta tgcttgcaca cttgttgaaa tacgacacaa
     1381 ctcaaggtcg tttcgacggt actgttgaag ttaaagaagg tggatttgaa gttaacggta
     1441 aattcatcaa agtttctgct gaacgtgatc cagaacaaat cgactgggct actgacggtg
     1501 tagaaatcgt tcttgaagct actggtttct ttgctaagaa agaagcagct gaaaaacacc
     1561 ttaaaggtgg agctaaaaaa gttgttatca ctgctcctgg tggaaacgac gttaaaacag
     1621 ttgtattcaa cactaaccac gacgttcttg acggtactga aacagttatc tcaggtgctt
     1681 catgtactac aaactgcttg gctccaatgg ctaaagctct tcaagataac tttggtgttg
     1741 ttgaaggatt gatgactact atccacgctt acactggtga ccaaatgatc cttgacggac
     1801 cacaccgtgg tggtgacctt cgccgtgctc gcgctggtgc tgcaaacatc gttcctaact
     1861 caactggtgc tgctaaagct atcggtcttg taatcccaga attgaacggt aaacttgacg
     1921 gatctgcaca acgcgttcca actccaactg gatcagttac tgaattggta gcagttcttg
     1981 aaaagaacgt tactgttgat gaagtgaacg cagctatgaa agcagcttca aacgaatcat
     2041 acggttacac agaagatcca atcgtatctt cagatatcgt aggtatgtct tacggttcat
     2101 tgtttgacgc aactcaaact aaagttcttg acgttgacgg taaacaattg gttaaagttg
     2161 tatcatggta cgacaacgaa atgtcataca ctgcacaact tgttcgtact cttgaatact
     2221 tcgcaaaaat tgctaaataa ttcttgagtt gatagaaagc aaggctttgt ggtcttgctt
     2281 tttatatgga aaaatggatg acacgatcat ccattctttt ttaattcttt ttcaaatgta
     2341 tttgaaaggg tagtgaaagt tagcttttct aaagtaagtg ggtgggtaaa ggaaagtcgg
     2401 aaggcatgaa gcataagccg acttgtcttt gatttactat tatagagagg gtcgcccagg
     2461 ataggaagat tatgatgcga aaggtgcaca cgaatctgat gggttcgccc tgtctttagc
     2521 ttgcaacgag ccaaggaagt cttgtttgag aattgcttta atctgcttac atgcgtttca
     2581 gcatattgcc cattttttgc atcaactatt ctttttctac gatcatggcg atcacgtcca
     2641 attttgtctc tgaaaacaag ttcttttctg ttgatatttc catcaactag agcccaatat
     2701 tctctagaaa tctctttttt ctccaataag cgattgagaa tgggcaggat aaaaggattt
     2761 ttggcaaaga gaactaagcc actggtttcc atgtccagac gatgaacgac atagcaggtt
     2821 tggccaacat aggtactgac atggttaaga agggcaattt cgtttggttg attaccatgc
     2881 gttttcatcc cctctggttt gtttacaata atcaagtgtt gatcttgata aacttcctgc
     2941 actaagtctg ggttgcccca agggatcgtc ttttgggaat aatcttcctc gtcaaaagtc
     3001 aactggcaaa catctccagg atttacgatt tcgttccagt ggacttcttc ttgatttatc
     3061 aaaatatgtt tcttgattct caaaaaatga cggatttttc tagggatgag gagttgttcc
     3121 tcaagtaatt gctttaccgt catttgaggt agagaggcgg gtaatgtaaa tgtgaattgc
     3181 atacagatat tgtaacaaaa aaagccctat ttggatagga aatagctaaa ttcttgtctt
     3241 cctatgatga agatgataaa ataaacgcat gaaattagat aaattatttg agaaatttct
     3301 ttctcttttt aaaaaagaaa caagtgaact agaggactct gattctacta tcttacgtcg
     3361 ctctcgtagt gatcgaaaaa aattagccca agtaggtccg attcgaaaat tctggcgtcg
     3421 ttatcatcta acaaagatta tccttatact aggtttgagt gcaggattgc tagttggaat
     3481 ctatttgttt gctgtagcca agtcgaccaa tgtcaatgat ttgcaaaatg ccttgaaaac
     3541 tcggactctt atttttgacc gtgaagaaaa agaggctggt gccttgtctg gtcaaaaggg
     3601 aacctatgtt gagctgactg acatcagtaa aaacttgcag aatgctgtta ttgcgacaga
     3661 agaccgttct ttctataaaa atgacgggat taactatggc cgtttcttct tggctattgt
     3721 cactgctgga cgttcaggtg gtggctctac cattacccaa cagctggcta aaaacgccta
     3781 tttatcgcag gatcaaactg ttgagagaaa agcgaaagaa tttttccttg ccttagaatt
     3841 aagcaaaaaa tatagtaagg agcaaattct aaccatgtac cttaacaacg cttattttgg
     3901 aaatggtgtg tggggtgtag aagatgcgag taagaaatac tttggagttt ctgcatcaga
     3961 agtgagtctg gatcaagctg cgactctggc agggatgctc aaggggccgg aactgtataa
     4021 tcccttgaat tctgtagaag attctactaa tcggcgcgat actgtcttgc agaatatggt
     4081 tgcagcagga tatattgata aaaaccaaga aaccgaagct gctgaagttg atatgacttc
     4141 gcaattgcac gataagtatg aaggaaaaat ctcagattac cgttacccct cttattttga
     4201 tgcggtggtt aatgaagctg tttccaagta taatctaaca gaggaagaga ttgtcaataa
     4261 tggctaccgc atttacacag agctggacca aaactaccaa gcaaatatgc agattgttta
     4321 tgaaaacata tcgctatttc cgagggcaga ggatggaacg tttgctcaat caggaagtgt
     4381 agctctcgaa ccgaaaacag ggggagttcg tggagttgtc ggtcaagttg ctgacaatga
     4441 taaaactgga ttccggaatt tcaactatgc aacccaatca aagcgtagtc ctggttctac
     4501 aattaagcct ttagttgttt atacgccagc agttgaagca ggctgggctt tgaataagca
     4561 gttggataac cataccatgc agtatgacag ctataaggtt gataactatg cagggatcaa
     4621 aacgagtcga gaagttccta tgtatcaagc cttggcagaa tcgcttaatc tacctgctgt
     4681 tgtcactgtt aatgatttgg gtgtcgacaa ggcttttgag gcaggcgaaa aattcggact
     4741 caacatggaa aaggttgacc gtgttcttgg tgtcgccttg ggaagcggtg ttgaaaccaa
     4801 ccctcttcaa atggctcaag catacgccgc ctttgcaaat gaaggtttaa tgcctgaagc
     4861 tcattttatt agtagaattg aaaatgctag tggacaagtt attgcgagtc ataaaaattc
     4921 acaaaaacgg gtgattgata agtctgtagc tgacaagatg accagtatga tgttggggac
     4981 tttcaccaac ggtaccggta ttagttcatc gcctgcagac tatattatgg cagggaaaac
     5041 tggaacaact gaagcagttt ttaatccgga gtacacaagt gaccagtggg taattggtta
     5101 tactccggat gtagtgatta gccactggct tggctttccg accactgatg aaaaccacta
     5161 tctagctggc tctacttcaa acggtgcagc tcatgtcttt agaaacattg ccaataccat
     5221 tttaccttat acgtcaggaa gtacctttac ggttgaaaat gcttataagc aaaatggaat
     5281 tgcaccagcc aatacaaaaa gacaagtaca aaccaatgat aatagccaga cagatgataa
     5341 tttgtctgat attcgagggc gtgcgcaaag tctagtagat gaggctagcc gggctatctc
     5401 agatgcgaag attaaggaaa aggctcaaac aatatgggat tcgatagtca atctatttcg
     5461 ctaagatgct tgtcaaagcc tagctttctt gttataatgg ataagatgga ggcgttatgg
     5521 cactaaaaaa agcaagccta gcttgtgcgg tttgtggttc gagaaactat tcaatcaaga
     5581 tcagcggaaa ccccaagcct acacgactag aagtaaataa attttgtaag cattgtggca
     5641 agtacactac acatagagaa acgagatagg agagagcgat gcgttttatt ggagatattt
     5701 ttagacttct taaagacaca acgtggccaa ctcgcaagga aagctggaga gattttcgtt
     5761 ctatcatgga atacacagct ttctttgtag taattattta catttttgac cagttgattg
     5821 tttcaggttt gattcgattt attaacattt tttagaagat tagtggagtt aattacacta
     5881 gaaatcttct atttatgaaa ggaaatatca tggatagttt tgataaagga tggtttgttt
     5941 tacaaactta ttctggttat gaaaataagg tgaaagaaaa tctattacaa cgtgcacaaa
     6001 cctacaatat gttggataat attctacgcg ttgaaattcc aacacaaaca gtgcaagttg
     6061 aaaaaaatgg aaagagaaaa gaagtagaag aaaatcgctt tccaggttat gttcttgtag
     6121 aaatggtcat gacagatgaa gcttggtttg ttgttcgaaa cacaccaaat gttacaggat
     6181 ttgtcggatc acacgggaac agatcaaaac caactccatt attggaacaa gaaattcgtg
     6241 acattttggt atctatggga caaacttttc aagaatttga tttcgatgtt gagattggtc
     6301 aaactgtacg tattattgat ggtgcttttg cagactacac tggtaagatt acagaaattg
     6361 ataataacaa agtgaaaatg attatctcta tgtttggtaa tgacacagtt gcagaagtaa
     6421 acctaaacca aatcgcagaa ttataaccct gagagaggct ggagcctctc ttttttgtgc
     6481 agtttaggag ctaaagggaa cagaatggag aaaatggaac aaatgtgttt tctaatctgt
     6541 tagactgtat ctagaaaggg gaaaattatg attaaagaat tgtatgaaga agtccaaggg
     6601 actgtgtata agtgtagaaa tgaatattac cttcatttat gggaattgtc ggattgggac
     6661 caagaaggca tgctctg
//
LOCUS       DAVLVP010000091         6507 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.091, whole genome shotgun sequence.
ACCESSION   DAVLVP010000091 DAVLVP010000000
VERSION     DAVLVP010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.091"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(76..1164)
                     /locus_tag="VYN69_001717"
     CDS             complement(76..1164)
                     /locus_tag="VYN69_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001860765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol dehydrogenase"
                     /protein_id="HEV1533949.1"
                     /translation="MRIFASPSRYIQGENALFENAKSILDLGNYPILLCDQLVYDIVG
                     KRFEDYLHRYGFHIVLALFNGEASDNEINRVVALAEKENCDSIIGLGGGKTIDSAKAI
                     ADLIEKPVIIAPTIASTDAPVSALSVIYTDEGAFDHYLFYSKNPDLVLVDTKVISQAP
                     KRLLASGIADGLATWVEARAVMQANGKTMLGQQQTLAGVAIAKKCEETLFADGLQAMA
                     ACEAKVVTPALENIVEANTLLSGLGFESGGLAAAHAIHNGFTALTGDIHHLTHGEKVA
                     YGTLVQLLLENRPKEELDKYIEFYKKIGMPTTLKEMHLDQVGYDDLIKVGKQATMEGE
                     TIHQMPFKISPSDVAQAIIAVDAYVNSK"
     gene            complement(1182..1850)
                     /locus_tag="VYN69_001718"
     CDS             complement(1182..1850)
                     /locus_tag="VYN69_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000395441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEV1533950.1"
                     /translation="MEFMLDTLNLDEIKKWSEILPLAGVTSNPTIAKREGSINFFERI
                     KDVRELIGSTPSIHVQVISQDFEGILKDAHEIRRQAGDDIFIKVPVTPAGLRAIKVLK
                     KEGYHITATAIYTVIQGLLAIEVGADYLAPYYNRMENLNIDSNSVIRQLALAIDRQNS
                     PSKILAASFKNVAQVNNALAAGAHAVTAGADVFESAFAMPSIQKAVDDFSDDWFVIQN
                     SRSI"
     gene            complement(1869..4307)
                     /locus_tag="VYN69_001719"
     CDS             complement(1869..4307)
                     /locus_tag="VYN69_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HEV1533951.1"
                     /translation="MVNTEVARTTIKTEYFGSLTERMNKYREDVLNKKPYIDAERAVL
                     ATRAYERYKEQPNVLKRAYMLKEILENMSIYIEEESMIAGNQASSNKDAPIFPEYTLE
                     FVLNELDLFEKRDGDVFYITEETKEQLRSIAPFWENNNLRARAGALLPEEVSVYMETG
                     FFGMEGKMNSGDAHLAVNYQKLLQFGLRGFEERARKAKVALDLTDPASIDKYHFYDSI
                     FIVIDAIKVYAKRFVALAKSLAENANPKRKKELLEIADICSRVPYEPATTFAEAIQSV
                     WFIQCILQIESNGHSLSYGRFDQYMYPYMKADLESGKETEDSIVERLTNLWIKTITIN
                     KVRSQSHTFSSAGSPLYQNVTIGGQTRDKKDAVNPLSYLVLKSVAQTHLPQPNLTVRY
                     HAGLDARFMNECIEVMKLGFGMPAFNNDEIIIPSFIAKGVLEDDAYDYSAIGCVETAV
                     PGKWGYRCTGMSYMNFPKVLLITMNDGIDPASGKRFAPSFGHFKDMKNFSELENAWDK
                     TLRYLTRMSVIVENSIDLSLEREVPDILCSALTDDCIGRGKHLKEGGAVYDYISGLQV
                     GIANLSDSLAAIKKLVFEEERISPSQLWHALETDYAGEEGKVIQEMLIHDAPKYGNDD
                     DYADKLVTAAYDIYVDEIAKYPNTRYGRGPIGGIRYSGTSSISANVGQGRGTLATPDG
                     RNAGTPLAEGCSPSHNMDQHGPTSVLKSVSKLPTDEIVGGVLLNQKVNPQTLAKEEDK
                     LKLIALLRTFFNRLHGYHIQYNVVSRETLIDAQKHPEKHRDLIVRVAGYSAFFNVLSK
                     ATQDDIIGRTEHTL"
     gene            complement(4458..5756)
                     /locus_tag="VYN69_001720"
     CDS             complement(4458..5756)
                     /locus_tag="VYN69_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000041357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1533952.1"
                     /translation="MSKMDVQKIIAPMMKFVNMRGIIALKDGMLAILPLTVVGSLFLI
                     MGQLPFEGLNKSIASVFGANWTEPFMQVYSGTFAIMGLISCFSIAYSYAKNSGVEALP
                     AGVLSVSAFFILLRSSYIPKQGEAIGDAISKVWFGGQGIIGAIIIGLVVGSIYTFFIK
                     RKIVIKMPEQVPQAIAKQFEAMIPAFVIFLSSMIVYILAKSLTNGGTFIEMIYSAIQV
                     PLQGLTGSLYGAIGIAFFISFLWWFGVHGQSVVNGVVTALLLSNLDANKAMLASGNLS
                     LENGAHIVTQQFLDSFLILSGSGITFGLVVAMLFAAKSKQYQALGKVAAFPAIFNVNE
                     PVVFGFPIVMNPVMFVPFILVPVLAAVIVYGAIATGFMQPFSGVTLPWSTPAILSGFL
                     VGGWQGVVTQLLVLVISTVVYFPFFKIQDRLAYQNEIKQS"
     gene            complement(5773..6081)
                     /locus_tag="VYN69_001721"
     CDS             complement(5773..6081)
                     /locus_tag="VYN69_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000238716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1533953.1"
                     /translation="MVKIGLFCAAGFSTGMLVNNMKIAAQSSGVEAEIEAFSQSKLAD
                     YAPNIDVALLGPQVAYTLDKSKEICDKCDVPIAVIPMMDYGMLDGKKVLDLALSLISG
                     "
     gene            complement(6127..6447)
                     /locus_tag="VYN69_001722"
     CDS             complement(6127..6447)
                     /locus_tag="VYN69_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1533954.1"
                     /translation="MEIIVPDQIIMGLILYAGDAKQHIYKALDYIKNGTCERCEEEIQ
                     LADAALLEAHNLQTKFLAQEASGTKTEITALFVHSQDHLMTSMTEINLIKEIISLRKE
                     LHKK"
BASE COUNT         2117 a         1357 c          955 g         2078 t
ORIGIN      
        1 tatttcaatc ttctatagaa ttcaatacag ggatcaataa aagactacca tttggaaaac
       61 agtagtcctt attgtttatt ttgaatttac ataggcatct acagcgataa tagcttgagc
      121 aacatctgaa ggcgaaatct taaacggcat ctgatgaatt gtctcaccct ccatagttgc
      181 ttgtttacca acttttatta aatcatcata tccaacttga tccaaatgca tttcttttag
      241 agttgttggc ataccaattt ttttgtaaaa ctcaatatac ttatcaagtt cttctttagg
      301 tctattttcc aataatagtt gtactaaagt tccataagct actttttcac catgtgttaa
      361 atgatgaatg tcacctgtca atgcagtaaa accattatga attgcatgcg ccgcagctaa
      421 tcctccactt tcaaaaccta gaccactcaa taaagtatta gcttcaacaa tattttctaa
      481 tgctggtgtc accactttag cttcacaagc tgccatagcc tgtaaaccat ctgcaaacag
      541 cgtttcttca catttcttcg caattgcaac tccagccaat gtttgctgtt gtcccaacat
      601 agtttttcca tttgcctgca taaccgcacg cgcctcaacc caagttgcta aaccatctgc
      661 aataccagac gctaataaac gcttaggggc ttgtgaaata acttttgtat caaccaaaac
      721 taaatctgga tttttagaat aaaatagata atgatcaaat gcaccttcat ctgtataaat
      781 aacagataaa gcagatacag gtgcgtcggt cgatgcaatt gttggagcaa taataacagg
      841 cttttcaatc aaatctgcaa tagcttttgc gctatcaatc gtctttcccc caccaagacc
      901 gataatacta tcacaatttt ctttctcagc caaggcaaca actcgattga tttcattgtc
      961 agaagcttca ccattaaata gcgccagaac aatatggaaa ccatacctat gtaggtaatc
     1021 ttcaaatcgt tttccaacaa tatcataaac caactgatcg cataatagaa tagggtaatt
     1081 tcccaaatcc aaaattgatt tggcattttc aaacaaggca ttttccccct gaatatatct
     1141 agaaggacta gcaaaaattc tcatatgtat ttcctctcta tctaaatgga acgactattt
     1201 tgaataacaa accaatcgtc agaaaaatca tcaaccgcct tttggataga tggcatggcg
     1261 aaagctgatt caaaaacatc tgctcctgct gtaacagcat gcgcacctgc agctaaagca
     1321 ttatttactt gtgctacatt tttaaaggat gcagctaaaa tcttactagg agagttctgt
     1381 ctatcaatag caagagctaa ttgacgaatg acagaatttg aatcaatgtt cagattttcc
     1441 attctattat aatatggagc taggtaatcc gctcctactt cgatagctaa taatccctga
     1501 ataactgtat aaatagctgt tgcagtgata tggtagccct ctttttttag cacctttatt
     1561 gcacgtaatc cagctggagt aacaggtact ttgataaata tatcatctcc tgcttgtctt
     1621 cgaatttcat gagcatcctt taagatgcct tcaaaatctt gagaaatcac ctgaacatga
     1681 atagagggtg tagagccaat caattctctt acatctttga ttcgttcaaa aaaattaata
     1741 gaaccctctc tttttgcaat agtgggattt gaagttaccc cagctagcgg caaaatttca
     1801 gaccactttt taatctcatc taaatttaat gtgtcaagca taaattccat aaaaagaacc
     1861 tctttatttt acaaagtatg ctcagtacgt cctataatgt catcttgggt tgccttagaa
     1921 agaacattga agaatgcaga gtatcctgca acacgaacaa ttaagtctct gtgtttttca
     1981 ggatgtttct gagcgtcaat caacgtctct ctggaaacaa cattgtattg aatatggtac
     2041 ccatgtaaac gattaaagaa tgttcgtaac aaagcaatta gttttaattt atcttcttct
     2101 ttggctaacg tttgaggatt tactttctga tttaagagaa ccccacctac gatttcatct
     2161 gttggtaatt ttgaaacaga ttttaaaaca gatgtagggc cgtgttgatc catattatgt
     2221 gatggtgaac aaccctctgc taacggtgta cccgcgttgc gtccatctgg agttgctaat
     2281 gttccacgtc cctgccctac gttggctgat atagaagatg ttcctgaata acgaattcct
     2341 ccaataggcc ctcttccata acgtgtatta ggatatttag caatttcatc aacataaatg
     2401 tcataagcag cagtaaccaa tttgtcagca taatcatcat cattaccata cttaggtgca
     2461 tcatgaatca acatttcttg aatgacctta ccttcttctc cggcataatc tgtttccagt
     2521 gcatgccaaa gttgacttgg gcttatacgt tcttcctcaa acaccaattt tttaattgca
     2581 gctaacgaat ccgacaaatt tgcaattcca acttgcaatc ctgatatata atcatatact
     2641 gctccacctt ctttaaggtg ttttccacga ccaatacaat catcagtcaa tgctgaacat
     2701 agaatatcag gaacttctcg ttccaatgat aaatcaatag aattttcaac aataacactc
     2761 attcgtgtca aatatcttag tgttttatcc caagcatttt ctaattcaga aaagttcttc
     2821 atatccttaa aatgaccaaa gcttggtgca aaccgtttac ccgaagccgg atcaattcca
     2881 tcattcatcg tgataagtag aaccttaggg aagttcatat aactcatacc tgtgcaacga
     2941 tagccccatt tccctggaac tgccgtttca acacatccaa tggcactgta atcataagca
     3001 tcatcttcca atactccttt tgcaataaaa gaaggaataa taatctcatc attattaaat
     3061 gcaggcatac caaaaccaag tttcatcact tcaatacact cattcatgaa acgagcatct
     3121 aaacctgcat ggtaacgtac agttagatta ggttgcggta gatgggtttg tgcaactgat
     3181 tttaatacca aataagataa tgggttaaca gcatccttct tatctcgagt ctgtccacca
     3241 attgtaacat tttgatataa aggacttcct gctgaagaaa atgtatgtga ttgactgcga
     3301 accttattaa ttgtaattgt cttaatccaa agatttgtca gacgttcaac aatgctatct
     3361 tctgtttctt taccactttc taaatcagcc ttcatatatg gatacatata ttgatcaaaa
     3421 cggccatatg aaagagagtg gccattagat tcaatttgta aaatacattg aataaaccaa
     3481 actgattgaa tagcttctgc aaaagtagtt gccggttcat atgggactct agagcaaata
     3541 tctgcaatct caagtaattc tttcttacgt ttaggatttg cattttcggc taaactttta
     3601 gcaagagcaa caaagcgctt tgcatatact ttaatagcat cgattacgat aaatatagag
     3661 tcgtaaaaat gatatttatc aatacttgct ggatctgtta aatctagagc tacttttgct
     3721 ttacgagccc gctcttcaaa acctcttaaa ccaaattgca aaagtttctg atagttaact
     3781 gctaagtgag catctccaga attcatctta ccttccatac cgaagaatcc tgtttccata
     3841 taaactgaca cttcttcagg taataaggca ccagctctag cacgtaaatt attattttcc
     3901 caaaacggag caatacttct aagttgttct tttgtttctt ctgtaatata gaaaacatct
     3961 ccatcacgct tttcaaaaag atccaactca ttgagaacaa attctagcgt atattccgga
     4021 aaaataggag catctttatt ggaagaagct tgatttcccg caatcataga ttcttcttca
     4081 atatagatag acatattttc caaaatttct ttcagcatat atgcacgttt taggacatta
     4141 ggttgttcct tgtatcgttc ataggcgcgt gttgctagaa ctgctctctc agcatcaata
     4201 taaggttttt tatttaaaac atcttctcga tatttgttca tcctttcagt aaggctgcca
     4261 aaatattctg ttttgattgt tgttcttgct acttctgtat ttaccatcgt aagcaccttt
     4321 ctttcgaaaa tatttttatt tctttcgaaa atataatacc gatttttata gaaattttca
     4381 agcttttaac tagaaaaatt ttaactctct aatttttagc acaaatgtgt gagtttaaca
     4441 gtaacacaca aatacctcta agattgtttg atttcatttt ggtaagctaa acgatcttgt
     4501 atcttaaaga atggaaagta aaccactgta gatatcacca atactaataa ctgagtcaca
     4561 acaccttgcc atccacccac caaaaatcct gatagaatag ctggtgtact ccaaggcaat
     4621 gttacccctg agaatggctg catgaaacct gttgcaatag ctccatatac tatcacagct
     4681 gcaagtacag gaacaagaat gaaaggtaca aacataactg gattcatgac aatcggaaat
     4741 ccaaatacaa ctggctcatt tacgttaaat attgctggaa aagctgcaac tttccctaag
     4801 gcttggtatt gttttgattt tgctgcaaaa agcatggcaa ctacaagacc aaacgtaatc
     4861 cctgaacctg atagaattaa aaatgaatct aaaaattgtt gagtaacaat atgtgcacca
     4921 ttttctaatg atagattacc agaggctaac atagctttat tagcatcaag attagataaa
     4981 agcagagctg tcactactcc atttactacc gattgcccat gaacaccaaa ccaccacaaa
     5041 aatgatataa agaatgcaat tccaatagca ccatacaaag atccagttaa accttgcaac
     5101 ggaacttgaa tagcagaata aatcatttct atgaatgttc cgccattagt caatgacttc
     5161 gctaaaatat atacaatcat agaagataag aaaattacaa atgctggaat cattgcttca
     5221 aactgtttgg caatagcttg tggaacttgt tctggcatct taataacaat ttttctcttt
     5281 ataaagaagg tataaatact tcctactacc aaacctataa tgatagcacc gataattcct
     5341 tggcctccaa accaaacttt actaatagcg tccccaatcg cctcaccttg tttagggata
     5401 taagatgatc ttagcaaaat aaagaatgca gatacagata gaactccagc tggtaaagcc
     5461 tctactccgc tattcttagc ataagaatag gcaattgaaa aacaagaaat tagacccata
     5521 atagcaaaag ttcctgaata tacttgcata aacggctctg tccaattagc tccaaaaaca
     5581 ctagcaatgc tcttatttaa tccttcgaac ggcaattgtc ccataatcaa gaacaaactg
     5641 ccaactactg tcaatggcaa aattgctaac atcccatctt ttagagctat aatgccacgc
     5701 atattcacaa acttcatcat cggtgcaatg attttctgaa catccatctt tgacataata
     5761 aatctccttt tcttacccac taatcaaaga tagggccaaa tctaatactt ttttcccatc
     5821 taacatacca tagtccatca tcggaataac agctatcgga acatcacact tatcacaaat
     5881 ttcttttgat ttatctaatg tataagcaac ttgtggaccc aatagtgcaa catctatatt
     5941 tggcgcataa tccgctaatt tagactgaga aaacgcctct atttctgcct caactccact
     6001 agattgcgct gcaattttca tattatttac aagcatacca gtagaaaaac ctgctgcaca
     6061 aaacaaacca atcttcacca ttatgttttc ctcctctatg ttaataacaa tgataatact
     6121 ctagtattat tttttatgaa gttcttttct caaactaata atttctttga ttaaattaat
     6181 ctccgtcata ctagtcatga gatgatcttg tgaatgaaca aagagagctg taatttctgt
     6241 ctttgtacca gacgcttcct gtgccaagaa ttttgtttgt agattatgag cttctaataa
     6301 ggctgcatca gctaactgga tttcttcttc acaccgttca catgtaccat tttttatgta
     6361 atctaacgct ttataaatat gttgtttcgc atcaccagca tataaaatta aacccatgat
     6421 aatttgatct ggaacaataa tttccataaa ttcctcctca tttcactatg taaaaagctt
     6481 ttttcatttg aaattttaaa attttct
//
LOCUS       DAVLVP010000092         6416 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.092, whole genome shotgun sequence.
ACCESSION   DAVLVP010000092 DAVLVP010000000
VERSION     DAVLVP010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6416
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.092"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            1..286
                     /locus_tag="VYN69_001723"
                     /pseudo
     CDS             1..286
                     /locus_tag="VYN69_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001822207.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            214..666
                     /locus_tag="VYN69_001724"
     CDS             214..666
                     /locus_tag="VYN69_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001841326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533955.1"
                     /translation="MEFNINSKESSQQLDILYKEVGVLSLGQKVVAMLDFLLAYSDYS
                     KDFRPLIIDQPEDNLDNRYIYRHLVQQFRDVKAQRQIILATHNATIVTNSMTDQVVIM
                     ESDGVNGWIESQGYVSEKYIKNHIINQLEGGKDSFKHKMSIYETALSE"
     gene            1485..1844
                     /locus_tag="VYN69_001725"
     CDS             1485..1844
                     /locus_tag="VYN69_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018379612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533956.1"
                     /translation="MSEQYRDIRKEVNLTADELKQIEKMMEVDNYRHFSPFVRDKILM
                     TDDKQLAAKEWFSLWQSQKFEQISRDVHLVLIIARENHQVTQEHVSILLTCVQELIAE
                     VNQVQSLSRGFREKYMR"
     gene            1854..2219
                     /locus_tag="VYN69_001726"
     CDS             1854..2219
                     /locus_tag="VYN69_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEV1533957.1"
                     /translation="MVYRYRTNLKKVFLTDSELHQLNERIAKSNCQNFSVYARKVLLN
                     PNMSFVTINTDTYDQLVFELRRIGNNINQIARAINQSRLISQEQLQELSKGVGELIKE
                     VDKEFQVEVKRLKEFHGSH"
     gene            2206..4056
                     /locus_tag="VYN69_001727"
     CDS             2206..4056
                     /locus_tag="VYN69_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEV1533958.1"
                     /translation="MVVTKHFATHGKKYRRRLIKYILNPDKTDNLKLVSDFGMSNYLD
                     FPSYEEMVEMYNVNFTNNDKLYEYRNDRQEKHQQNIHAHHLIQSFSPEDNLTPEEINR
                     IGYETIMELTGGRFRFIVATHTDKDHIHNHILINAIDCNSDKKLIWNYALERNLRMIS
                     DRISKMAGAKIIEKRFSYRDYQKYRATSHKFELKQRLYFLMQQSKSFDDFLEKAEQLH
                     VHIDFSQKHSRFMMTDRAMTKPIRGRQLSKRDLYDEDFFRMHFTKQEIASRLEFLLNC
                     VNSLEGLLTKSKELNLTIDLKQKNVIFILEENGKQFSLSHKKISDEKLYDVNFFQDYF
                     KNKEVGVSEGIENLQAQYRAFQEERDKEKVSTEEIEEAFETFKEKRDAVHEFEVKLTE
                     HQIEKLVDEGIYIKVSFGINQSGLIFIPNYQLDIMEEENQKKYKVYIRETTSYFVYNK
                     EHSDKNQYIKGRTLIRQLTNDSRVIPYRRPTVERLQEKISEISLLIELTETDKKYQDI
                     KDNLVSEIAELDIKLTQTNEKIATLNKMAEVLINSKSEGSGSQKLARHEFSKLNMTES
                     TTLEQVNEELLKLQQEFGNVLDEYEKTIRKLGQLFKVFDECINKEIMNEI"
     gene            4312..5744
                     /locus_tag="VYN69_001728"
                     /pseudo
     CDS             4312..5744
                     /locus_tag="VYN69_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886556.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(5982..6116)
                     /locus_tag="VYN69_001729"
                     /pseudo
     CDS             complement(5982..6116)
                     /locus_tag="VYN69_001729"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
BASE COUNT         2222 a         1028 c         1265 g         1901 t
ORIGIN      
        1 caagaatcat gggttaatca atggttaagt gaaaatatta taagtgagaa agatggaagt
       61 tatcaacatt cactgactat aatgaaggat ttcaataatc aaaaaatagt taactatatt
      121 gctcatttca atagttatga cattttgaaa aaaggttctc acttattagg tgcatataaa
      181 cgaaaaattt tttctaaaga aaatacacga tttttggagt ttaatattaa ctcgaaagaa
      241 tcttcgcaac aacttgatat tctctataaa gaagttggtg tattaagttt gggacaaaaa
      301 gttgtagcca tgcttgattt tttattagca tatagtgatt attctaaaga cttcagacca
      361 ttgattattg atcagcctga agacaatcta gacaatcgtt atatttacag gcatttagtt
      421 cagcagttta gagatgtgaa agctcaacgt caaattattt tagcaacaca taatgctaca
      481 attgtaacaa attctatgac agatcaagtt gttattatgg agtcagatgg agttaacgga
      541 tggattgaat cacagggata tgttagtgaa aaatatataa aaaatcatat catcaatcaa
      601 ttagagggag gaaaagattc cttcaagcat aaaatgtcta tatatgagac ggctttatca
      661 gagtagagtc agaaaaagta ggttagaaat ttagcctact tttttctttg tccgacaggc
      721 atagtgtaca tctgaggtcc aagtcctctg tggatatttg ctgcagatga aaccaatagc
      781 gactcctaag cctgaatatc gtgaggtagg ggggatagga aggaattagc gaaatcaagg
      841 ttctacaaac agaatcgtga cttgaagcca tatatagcgg atgaggaact ctaaaatcca
      901 aataggtgtc gtaacctata tacgtaaatt acgagagtaa actaggaaag atgtacggct
      961 tattccgtga gcgtttagga cgtagtacaa cgaatcatgg gagtcagctg aacacatagt
     1021 attgaagaaa tttctgtaat ggaaatggag cgaagaagtg aacaattaaa tgaatacctc
     1081 tctaattaaa tttgtcaatt ctaattcctg gtatgaaaag acagtgacct aaaaatgtaa
     1141 acgatgggag ctgatcataa atataggacg gtacatgcag tggtgttaga gattagtccc
     1201 tacttgattt gtgataactt ccccaacttt cttctgctat acttttctca acttttaaaa
     1261 atccaactaa gaattttacc tgggagtttg ggggcgtagc caccaagtta tcttatggtt
     1321 agctgtcaaa actggaaggt tttggttagc tggcgatatg attttttggg tattgtgacc
     1381 acaatgcctg agctcgcaaa gaccgaacaa ggactttaag aaatttcccg tatgacacac
     1441 ggggtcgggg tttacccgag aaaggatgaa agtgaggaaa agagatgtct gaacagtacc
     1501 gtgacattcg aaaagaggta aatctaaccg cagatgagct aaaacagatt gaaaaaatga
     1561 tggaggttga taattatcgt cacttttcac cattcgtcag ggataaaatt ttgatgactg
     1621 atgacaaaca gttagctgcc aaagaatggt tttccctttg gcagtctcaa aagtttgaac
     1681 agatcagtcg agatgtgcat ttggttttga ttatcgcaag agagaatcac caagtgactc
     1741 aagaacacgt gtcaatctta ttgacctgcg ttcaagaatt gattgcggaa gtcaatcaag
     1801 tacagtcact cagtcgtggg tttcgtgaaa aatacatgag ataggaggct actatggttt
     1861 atcgttatcg taccaatctc aaaaaagtat ttctaacaga ttcagaatta caccaattga
     1921 atgaacggat tgctaagagc aattgtcaaa atttttcagt ctatgccaga aaagttttgc
     1981 ttaatcccaa tatgtcattt gtcacgatta acacggatac ttatgaccag ttagtttttg
     2041 aattgagacg gatcggaaat aacattaatc aaattgcgcg tgctatcaat caaagccgtc
     2101 tgatttctca ggaacagtta caagaattga gtaaaggagt cggagagttg atcaaggaag
     2161 tggacaaaga atttcaagtg gaagtgaaaa gattgaagga gtttcatggt agtcactaag
     2221 cattttgcga cacacggaaa aaaatatcgt aggcgtttaa ttaagtatat cctcaatcct
     2281 gataaaacgg acaatttgaa attagtatct gattttggta tgagcaatta cttagacttt
     2341 cctagctatg aagaaatggt agaaatgtac aatgtcaact ttaccaataa tgacaagttg
     2401 tacgaataca gaaatgaccg acaagaaaaa caccaacaaa atattcatgc ccatcacctc
     2461 attcaatcat tttctcctga agataatctg acacctgaag aaattaaccg cattggttat
     2521 gagaccatta tggaattaac aggaggccgt tttcgtttca tcgtagcgac tcatacagac
     2581 aaagatcata ttcataatca catcttaatc aacgccattg actgtaattc agataaaaag
     2641 ttgatatgga attatgcctt ggaacgaaac cttcgtatga tttcagaccg catttctaaa
     2701 atggctgggg cgaaaattat tgaaaagcgt ttctcttacc gtgactatca aaaatataga
     2761 gcgactagcc ataagtttga actaaagcaa cgtctttatt ttttgatgca gcagtcaaaa
     2821 tcctttgatg attttttaga aaaagcagag caattacatg ttcatattga ttttagtcag
     2881 aagcatagtc gattcatgat gacggatcgg gcaatgacaa aaccaattcg aggacgtcaa
     2941 ctcagtaaac gagatttata tgatgaagat ttttttagaa tgcattttac caagcaagag
     3001 attgcaagtc gtttagaatt tttgttgaac tgtgtcaatt ctttagaagg gttactaaca
     3061 aaatcaaaag aattgaatct aaccattgac ttaaaacaaa aaaatgtaat ctttatactt
     3121 gaagaaaatg gaaagcaatt cagtctgagt cataaaaaaa taagtgatga gaaattatat
     3181 gatgtcaatt tttttcaaga ttattttaaa aataaggaag taggtgtttc agaaggaata
     3241 gaaaatctac aggcacagta ccgtgctttt caagaagaac gagataagga gaaggtatct
     3301 actgaagaaa ttgaggaagc ctttgagaca tttaaggaaa aacgagatgc cgttcatgaa
     3361 tttgaagtaa aacttacaga acaccaaatt gagaagttag ttgatgaggg gatttatatc
     3421 aaagtgtcct ttggtataaa tcagagtggt cttattttca ttcctaacta tcaactggat
     3481 attatggaag aagaaaatca gaaaaaatat aaagtctaca ttcgtgagac aacctcatat
     3541 tttgtctata acaaagaaca ttcggataag aatcagtata tcaaagggag aaccttgatt
     3601 aggcagctaa ccaacgatag tcgagtaata ccatacagaa gaccgactgt tgaaagactt
     3661 caagaaaaga tttctgagat tagcctcttg attgagttaa ctgagacaga taagaaatac
     3721 caagacatta aagacaactt ggtttccgaa atagcagagc tagatataaa actgactcaa
     3781 acaaatgaaa aaattgccac cttaaataag atggcggaag tacttatcaa ttctaagagt
     3841 gaaggttcgg gtagccaaaa actagctagg catgaatttt ctaaattgaa catgacagaa
     3901 tcaacaactt tagaacaagt caatgaagaa cttttgaagt tacaacagga atttggtaat
     3961 gttcttgatg agtatgagaa aacaataaga aagcttggac aattatttaa ggtttttgat
     4021 gagtgtatca ataaagaaat tatgaatgaa atataaatca gactgaagac aaactcaaat
     4081 ttttttggat atcttttctg tttatggttg ccaatatcgg tttaacttct aaaacaaaca
     4141 ttaataaaaa tgcattcaaa gacgatttta cctattttac gattacgctt cgaccccaaa
     4201 tcattaaagt tgtctatgct ctatcattgc atcactggac ttgtcaagag ttaagataat
     4261 tcggagtcca ctgaaaaagt catcgtatga tggtatacta aagatgaggt gatggtatgc
     4321 ttgataatcg atgttagtcc ctattctact ctgtgtgata ttgttgttcc caaaactcat
     4381 tttttacgac agctaatgga actctgtgat tttagtttta tttatgacga gctggaaaag
     4441 aattatcaac ctgacttcgg atgtcgttcc tactctctcc ttatcatgat gttcaagtat
     4501 ctcttattaa aagacatcta taagttgtcc gatgtggatg tggtggagcg ctccttttct
     4561 ggtatgacct tcaaatactt ccttggtcta gctcctgtta ttgaaccgtc ctcattgact
     4621 aaattccgaa aacttcggaa caaagatgaa cgattacttg atttgctcat cgctaaatct
     4681 gttcagattg cgatagagct cggtctgatt aaaagtaaca ttctcattgt ggatgcgact
     4741 cataccaaag ttcattacaa tcacaagaaa cctcaagaag tcctccgaga acgctcaaaa
     4801 gccttgcgta aaacgattta tcagtattcg gaatatatca aggcagaatt tcctagtaag
     4861 cctcaggaag atactctagt agctgaatta agatatacac aagaagtaat atcagtcctt
     4921 gagaagcatg acgaattgac tggaatacca gctatctcac aaaattcaat taccttaaag
     4981 aagctgtaga ggatgactta gagcacttgg aatcctctgt caaagaagaa gcaagaattg
     5041 gacataagtc agccgatagt tccttttacg ggtacaaaga gcattttgcc atgaccgatg
     5101 aacggattat tactgcctgt gtggtgacat cgggtgaaaa aagtgatggt cctgttttag
     5161 aagaactcta tcacaaatct aaggataatg gcgtgacgat tgaagctatt gttggagata
     5221 gggcctactc tgggaaggat aatatgcaat ttaccaagaa agaacgtgtg cattgagtcg
     5281 ctaggccata tccaactatt tcaaaaggct ttcgtaggaa ggaagatgag tttttctata
     5341 acaaggacgc aggtctcttt gtttgtccag aagggcatat ggcactaaag aaggaaaaga
     5401 caggaagaag gacggctcag gaaacccact attttgacat caagcactgt caaatatgcc
     5461 cttcaaaagt gggctgttat aaagaagggg ctaagtctaa gacttatttg atacccctta
     5521 agagcgatga acatattttc cagaagaggt ttcaggaagc gccctatttt aaagaaaagg
     5581 ccaaacatcg ctacaagata gaagccaaaa atgcggaact tgaaaacaga catggtctta
     5641 atgtagctag agcaccaggt cttttaaaca tggagttaca agccgccaca accatatttg
     5701 tggttaatat gaagaggatt ataactctaa taaaccaaaa ataaggaaaa gcaaagagga
     5761 atctatccaa gcaggagata gatccctctt ttttcatttt agttgaactg tagagcttac
     5821 tttttcagtg gactcgatga ttagtgacta tttttttcaa actctattag ttattggtac
     5881 cctaatgccg aatggagata tttaggattg taatagattt caatatattt aaagatttcg
     5941 ttcaatagtt gcgaaattgg catcattgac aagttctctt tttatgtttt gtaaaaggat
     6001 tccatgggtg cattatcata tggattgcct tttcgactca ggctagactt gcatcttttc
     6061 tgtttaagga gttcttgaaa tctagctcca gtatattgag atccttgatc ggtatggccg
     6121 actagttctt atttgagatt ttctctatga taagcttgat tgaaaacatt cgttaccaac
     6181 ttatcttgca ttcgatttcc cattgcctaa ccaacaattt ttctgctata aacatatata
     6241 taatgataaa taaagagtac ctgcttttgt cggaatataa gttaaatccc ccagccagat
     6301 ttcattttta ccttttgttt gaaaacactg attaatcaaa ttgggtcgtg ttaatgagga
     6361 atgccgacgg ctatagtact tgtatttata gcggattcct ttagcataga gacaaa
//
LOCUS       DAVLVP010000093         6333 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.093, whole genome shotgun sequence.
ACCESSION   DAVLVP010000093 DAVLVP010000000
VERSION     DAVLVP010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.093"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            114..278
                     /locus_tag="VYN69_001730"
     CDS             114..278
                     /locus_tag="VYN69_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEV1533959.1"
                     /translation="MGIIANVFAGLVGAYAGQSLLGSWGPSIAGMALLPSIVGAAIVI
                     TVVSFFTGRK"
     gene            362..928
                     /gene="amaP"
                     /locus_tag="VYN69_001731"
     CDS             362..928
                     /gene="amaP"
                     /locus_tag="VYN69_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000030212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEV1533960.1"
                     /translation="MSKAKKICFIIFCILILTIFLPVLIDYHQVSDLGIHLLSWRQNS
                     VVEFYLARYVFWGTVVLSTLVLLSILVVMFYPKRYLEIQLETKNDTLKLKNSAIEGFV
                     RSLVSDHRLIKNPTVHVNLRKNKCFVHVEGKILPSDNIADRCQIIQNEITNGLKQFFG
                     IERQVKLEVAVKNYQPKPQNKKTVSRVK"
     gene            940..1110
                     /locus_tag="VYN69_001732"
     CDS             940..1110
                     /locus_tag="VYN69_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEV1533961.1"
                     /translation="MEWLKQYRYPIIAGLIGVFLACLIVSFGFFKTIFVLILGALGVA
                     AGLYIEKNYIDK"
     gene            1149..1757
                     /locus_tag="VYN69_001733"
     CDS             1149..1757
                     /locus_tag="VYN69_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000064115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEV1533962.1"
                     /translation="MSNEKNTNTNVEKKDATVVAHEIKGELTYEDKVIQKIIGLSLEN
                     VSGLLGIDGGFFSNLKEKIVNSDDVTSGVNVEVGKTQVAVDLNVIVEYQKNVPALYSE
                     IREIVSSEVAKMTDLEIVEINVNVVDIKTKEQHEADSVSLQDRVSDVAESTGEFTSEQ
                     FEKAKSGLGSGFSTVQEKVSEGVEAVKGAANGVVSHENTRVN"
     gene            1788..1991
                     /locus_tag="VYN69_001734"
     CDS             1788..1991
                     /locus_tag="VYN69_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEV1533963.1"
                     /translation="MSVEEKLNQAKGSIKEGVGKAIGDEKMEKEGAAEKVVSKVKEVA
                     EDAKDAVEGAVEGVKNMLSGDDK"
     gene            complement(2101..2373)
                     /locus_tag="VYN69_001735"
                     /pseudo
     CDS             complement(2101..2373)
                     /locus_tag="VYN69_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012514870.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            2577..2864
                     /locus_tag="VYN69_001736"
     CDS             2577..2864
                     /locus_tag="VYN69_001736"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1533964.1"
                     /translation="MIVSLKTKSRKAKDMAESIQGWLAQFLVNLFKSITFDCGKEFSK
                     WKDISNHHDSESFFANLGCPRQRCLNEHSNRLLRCHDLPKQTDFNEVSQEF"
     gene            complement(3142..3912)
                     /locus_tag="VYN69_001737"
     CDS             complement(3142..3912)
                     /locus_tag="VYN69_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000229327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEV1533965.1"
                     /translation="MVEIIYFLIIIIASGLGSISGMGGGIIIKPLMDSFGYHSVSDIA
                     FYSSFSVFIMAIISTTKRFSQSKEIKWRLIFTVSFSSVLGGFLGHLIFQVLLSQLSVR
                     LVSIVQMILLFVMLLVSFVLTDFKKTYQFDKIGFYMICGLLLGLISSFLGIGGGPLNV
                     SLLMVFFSISIKEATMYSLAIIFFSQLSHLATIVVVTGLNQYHLAPVPVIFLASICGG
                     VLGTVVSKVLPENWVRYCFKGMLFFVMGMTLYNLFHIL"
     gene            complement(3927..4775)
                     /locus_tag="VYN69_001738"
     CDS             complement(3927..4775)
                     /locus_tag="VYN69_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000782422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HEV1533966.1"
                     /translation="MKMKKIKGGTFMMGTNSEEGFLDDFEGPQVAVSVKDFSIADTPV
                     TNQEFAQFVKETGYKTLAERQEWSFVFILFVPEAEREGYPHPAGAPWWLQVSNACWKH
                     PYGENSNLVGLEDHPVVYVALEDALAFCNWSGMSLPTEAQWEYAARGGRQSEYPWGDT
                     LLEGGYYHANTWQGRFPYENTALDGFIGTAPVYEFLPNDFGLYQMIGNVWEWCRNPRY
                     TLLASFNEDDYELPKYGIQDEEYAIRGGSFLCHCSYCNRYRVAARNGCISTSTSSHLG
                     FRCLKE"
     gene            complement(4772..6247)
                     /locus_tag="VYN69_001739"
     CDS             complement(4772..6247)
                     /locus_tag="VYN69_001739"
                     /EC_number="3.1.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HEV1533967.1"
                     /translation="MVQTKQPNIILIVVDQMRADALSLNSKDKLVSTPTLDMMASVGY
                     NFENAYSPVPSCVPARAALLTGLDQDKSGRVGYQDEVPWNFTNTLPKVFKDMGYQTEC
                     IGKMHVFPSRQRLGFDHVLLHDGYLHVDRKYDKAYGSQFDYASDYLAFLKGKVGYDVD
                     LIDDGMDCNSWEARPWDKDEKLHPTNWVVSESISFLQRRDPTVPFFLKMSFEKPHAPL
                     NPPKYYFDIYMERLPQFLDLHIGNWEVLEKQIPSIYALRGKLKEDDQRRMVAAYFGLI
                     THIDHQISRFLTALKEFRHDKDTIIWFVSDHGDQLGEHYLFRKGYPYQGSIHIPSFIY
                     DPAGLIAGNRGTIKQLVKIQDIFPSLVDLAGGTTTDELDGRSVKNLLFGQYEGWRTEF
                     HGEHALGKDSSQYILTDQWKFIWFPVLNHYQLFDMKKDPHEMNDLYPSEKYQPIVRQM
                     KKKLVDFLRYREEGFVVDEELVPVELSKITPTLTKTGDSQS"
BASE COUNT         2191 a         1188 c         1035 g         1919 t
ORIGIN      
        1 aaattgattt tttgatatta aaataaaaaa ggagggtagg cagtgttgtg atcaattatt
       61 gctggaggtc ttattggtct cttggcaggt aaaatcacta aaaaagtagt tctatgggaa
      121 tcatcgcaaa tgtattcgct ggtttagtcg gggcatatgc aggacaatct cttttaggta
      181 gttggggtcc atcaatcgct ggaatggctt tgctcccatc tattgtaggt gcagcgattg
      241 tgattactgt agtgtcattc tttacaggta gaaagtaaac ttttcgccag taaagttagc
      301 aaactatttt taaatcaatg acgggaaaaa tagtttaaat gttaaatcga aaggattgta
      361 tatgtcaaaa gcaaagaaaa tatgtttcat tattttctgt attttaatct tgacaatttt
      421 ccttcctgtt ttgatagatt atcatcaagt tagtgatcta ggtattcatc tacttagctg
      481 gagacagaac tccgtagttg aattctatct tgctagatat gtcttttggg ggacagtggt
      541 tctatcaact ttagttttat tatccatttt agttgtgatg ttttatccta aacgttactt
      601 ggaaatccaa cttgaaacta aaaacgatac attaaaatta aagaattcgg caatcgaagg
      661 ttttgttaga agtttggtga gtgatcatag attgatcaag aacccaactg ttcatgtaaa
      721 tttacgaaaa aataaatgtt tcgttcatgt agaaggtaaa attcttcctt cagacaacat
      781 cgctgacaga tgccaaataa ttcaaaatga aataactaat ggattgaagc agttttttgg
      841 tattgagcgt caagtaaaac ttgaagttgc agtaaaaaat taccaaccaa aacctcaaaa
      901 caaaaagact gttagtcgtg tgaagtaagg aagtaaaaaa tggaatggct taaacaatat
      961 cgatatccaa ttatcgctgg tctcataggc gtatttctgg cttgtttgat tgtctccttt
     1021 ggcttcttca aaacaatatt tgtattgatt ttaggagcac tgggagttgc agctggatta
     1081 tatatcgaaa aaaactatat agataaataa aaaaataaaa attactaatt taattaaagg
     1141 agtttcatat gtcaaacgaa aaaaacacaa acactaacgt agaaaagaaa gatgctactg
     1201 ttgtagctca cgaaatcaaa ggggaactta cttacgaaga taaagttatc caaaaaatca
     1261 ttggtctttc actagaaaac gtttcaggtc ttttgggaat cgatggtggt ttcttctcaa
     1321 atcttaaaga aaaaatcgtt aacagcgatg acgtaacaag tggtgttaac gtagaagttg
     1381 gtaaaacaca agttgcagtt gacttaaacg ttattgttga gtaccaaaaa aatgttccag
     1441 ctttatattc agaaatcaga gaaatcgtat cttcagaagt tgctaaaatg actgacttgg
     1501 aaattgttga aatcaacgta aacgttgtcg acatcaaaac taaagaacag catgaagcag
     1561 actcagtaag ccttcaagat cgcgtatctg acgttgctga atcaacagga gaattcactt
     1621 cagaacaatt cgaaaaagct aaatctggtc ttggatctgg tttctcaact gttcaagaaa
     1681 aagttagcga aggtgtagaa gctgttaaag gtgcagcaaa tggtgtagta tctcacgaaa
     1741 acactcgtgt aaactaagat aaaataaata taacaggaga aattatcatg tcagtagaag
     1801 aaaaattaaa tcaagctaaa ggttctatta aagaaggtgt tgggaaagcc atcggtgatg
     1861 aaaaaatgga aaaagaagga gcagctgaaa aagttgtttc taaagtaaaa gaagttgccg
     1921 aagacgctaa agacgctgta gaaggtgctg tagaaggtgt taaaaacatg ttgagtggcg
     1981 acgataaata aggttaaaag ttactttatc tttttggtaa cattagtcaa aagagtctga
     2041 gtcaagatga ttctcagaaa acaaaaagct agagattccc aattgcggaa ctctagcttt
     2101 ttaattttgc ctctttctct tattatattt cagcaggttg ttggccatga gtatgaatcc
     2161 catgtcaatt ctcacttgac gcttacctct cagatgacat ctcttataac ccaaacaaac
     2221 ctttatctgc ccaaagacag atttcatatc aatcttacgt ttagcgaaaa tttgtctacc
     2281 cttggaagat aaaagtgcct gatattcttt agtttttaaa cactggtaac gttcattcat
     2341 atacagtccc ttttgagggg ctgattcagg ttcataatcg cagtcaacat tgatttcaag
     2401 gctgtttgct ttctatctcc ccttttttat gatgtatttt cgtacaggtt cttcaatagt
     2461 ttctatagta aaatgaacca aaaatagtac acattacagt ataatgttct tgtggcatat
     2521 tcattagatt ttcgtaaaaa gttctctcct atttgtgagc gaacaggtat agtcaattga
     2581 ttgtcagttt gaagaccaag agtcgtaagg caaaggatat ggcagaatcc atccaagggt
     2641 ggctggcaca atttctagtg aacctcttta aatctattac gtttgactgt ggaaaagagt
     2701 tttcaaaatg gaaagatatt tccaatcatc acgacagtga gagctttttt gcgaatctag
     2761 gatgtccaag acagcggtgt ctcaacgaac attcaaatag acttttaagg tgtcatgact
     2821 tgccaaagca gacagatttc aacgaagtca gtcaagaatt ttaggctgga caaaaactca
     2881 atttcaggag aaaatgaaat aaatattccc acaataaaac gcatagtatc aaagtttttc
     2941 caatacctga tattatgcat tttttaattt taaagacttt ttcccagcct caagaaatca
     3001 catcccacct aaatcctttg gataccaaat acctcatcag gtattttctg agttggtaag
     3061 actgtctagc ttaatttgac aaatggaggg cgttaaatcc agtttttaca ggaagtctat
     3121 ttaaatattt tcacactgac cttacaaaat atgaaataaa ttatataaag tcattcccat
     3181 gacaaagaat aacatccctt taaaacagta tcttacccaa ttttcaggta agacttttga
     3241 tacaaccgtc cccaataccc ctccgcatat agaagcaaga aaaataactg gaacaggtgc
     3301 taaatgatac tgatttaaac ctgtaaccac aacaattgta gccaaatgag acagctggga
     3361 aaagaaaata attgcaagag aatacatcgt tgcttctttt atcgaaatag aaaaaaacac
     3421 catcaataaa gatacattga gcggaccacc accaatccct aaaaaactag aaatcaagcc
     3481 caatagaaga ccacaaatca tatagaatcc tatcttatca aattgataag tttttttaaa
     3541 atctgttaaa acaaatgata caagtagcat cacaaatagt aaaatcattt gaacaataga
     3601 tactagccgt actgataatt gagataacaa aacttgaaaa ataagatgac ctagaaaccc
     3661 tcctaaaaca gaagaaaagg ataccgtaaa aatcaatctc cattttatct ctttactttg
     3721 tgaaaatctt ttggttgttg aaataatcgc cattataaaa acagagaaac tagaataaaa
     3781 agcaatatca ctgaccgaat ggtatccaaa actatccata agtggcttaa ttataattcc
     3841 accacccatg cctgatatag aacccaatcc actagctatt ataatgatta gaaaataaat
     3901 aatctcaacc actttttaag tccatcctat tctttcaaac atctaaaacc tagatggcta
     3961 ctagtggaag tagaaataca tccatttcta gcagctactc ggtatcgatt acaataagaa
     4021 caatgacata aaaaggaacc tcctctgata gcatactcct catcttgtat cccatatttt
     4081 ggtaactcat agtcatcttc attaaaacta gctaataaag tatatctcgg attgcgacac
     4141 cattcccaaa cattcccaat catttgatat agaccaaaat cattaggtaa aaattcatag
     4201 acaggagccg ttcctataaa tccatctaag gcagtatttt cataaggaaa tcttccttgc
     4261 caagtattgg catgataata tccaccttct aaaagagtat ctccccaagg atattcagat
     4321 tgtcttcctc ctctagcagc atattcccac tgtgcttcag tcggtaaaga cattccgctc
     4381 caattacaga aagccaatgc atcttctaaa gcaacataaa ctactggatg gtcttctaaa
     4441 cccactagat tactgttctc accgtaggga tgtttccagc aagcgttcga aacttgtaac
     4501 caccacggag ccccagcagg atgaggatat ccctcccttt ctgcctctgg aacgaacaat
     4561 ataaaaacaa aagaccactc ttgtcgctca gctagtgttt tatagcctgt ttctttcaca
     4621 aattgagcaa attcttgatt cgtaactgga gtatccgcaa tggaaaaatc ctttacacta
     4681 acagcgactt gaggaccttc aaaatcatct aaaaagccct cttcagaatt tgttcccatc
     4741 ataaatgtcc cacccttgat ttttttcatt ttcatgattg actatccccc gttttcgtta
     4801 gagttggcgt tattttagaa agttcaacag gaactaactc ttcatccaca acaaaacctt
     4861 cttctctata tctaagaaag tctactaatt ttttcttcat ctgacgaaca ataggttgat
     4921 atttttcact tggatacaaa tcattcattt catgcggatc ttttttcata tcaaataact
     4981 gataatgatt taaaacagga aaccagataa atttccactg gtctgtcaaa atatattgac
     5041 tagaatcttt tcctaaagca tgttcaccat gaaattctgt tcgccacccc tcgtactgtc
     5101 caaataataa attcttaaca ctccgtccat ctagttcatc tgtagtagtt cctcctgcca
     5161 aatccacgag tgaaggaaaa atatcttgaa ttttcactaa ttgcttgatt gttcccctat
     5221 taccagctat caatccagcc ggatcgtaaa taaacgaagg aatgtggata cttccctgat
     5281 aaggatatcc ctttctaaac agataatgtt caccaagctg atctccatga tccgaaacaa
     5341 accagataat cgtatcttta tcatgacgaa attcttttag ggcagtcaaa aagcgactaa
     5401 tttgatggtc tatatgagta atcaatccaa aataagcagc aaccattctt ctttgatcat
     5461 cttcctttaa tttccctctt aaagcataaa tagacggaat ttgcttttct aatacttccc
     5521 aattcccgat atgcaaatcg agaaattgag gcaagcgttc catatatata tcaaaatagt
     5581 attttggggg atttaatgga gcatgtggtt tttcaaaaga cattttgaga aaaaaaggaa
     5641 ccgttggatc tcttctttgt aaaaatgaaa tggactcact cacaacccag tttgttggat
     5701 gtaacttctc atctttatcc caaggtctag cttcccaaga attacaatcc attccgtcat
     5761 caatcaaatc aacatcataa ccaactttac cctttaaaaa ggctaaatag tcactagcat
     5821 aatcaaattg acttccataa gccttatcat actttctatc aacatgtaga tacccatcgt
     5881 gaagtaagac atgatcaaac ccaagtctct gtctggaagg aaaaacatgc atcttaccaa
     5941 tacactctgt ttgataaccc atatctttaa agacttttgg aagagtatta gtaaaattcc
     6001 aaggcacctc atcttgataa ccaactcgtc cacttttatc ttggtctaag ccagtcaata
     6061 aagctgccct agcaggaacg caagaaggaa ctggagaata ggcattttca aaattataac
     6121 ctacactagc catcatatct aatgttggag tgctaactag cttatcctta ctattcaagg
     6181 ataaggcgtc tgctctcatt tgatctacaa caatcaaaat aatatttggc tgttttgtct
     6241 gaaccataaa atctcctttc taatatggca aaagaggcac aagaagatat ctacctttac
     6301 tgcacccctt tctatatcaa tctctctata taa
//
LOCUS       DAVLVP010000094         6281 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.094, whole genome shotgun sequence.
ACCESSION   DAVLVP010000094 DAVLVP010000000
VERSION     DAVLVP010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6281
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.094"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..216
                     /locus_tag="VYN69_001740"
     CDS             <1..216
                     /locus_tag="VYN69_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533968.1"
                     /translation="GGKRLNTSIRSAYSGSDITPVYSLGSGSRIVMYYNGGGDNYIGS
                     GTRLAMAPQFGNHVRIHTSGSWSPDSY"
     gene            312..1076
                     /locus_tag="VYN69_001741"
     CDS             312..1076
                     /locus_tag="VYN69_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533969.1"
                     /translation="MEFFICNLVRVVQSPRFYMSLFLTLLCMSLGNFLAFNGIYKIEG
                     LSIFFAASSIRGFSPISLVAALICTLPYSSQIIEDAESHFLTAQLCRISKKRYLAIVG
                     STAIISSFLVFFLPYLLLLGINLLVTPYQEIYIGDYSGALKELFDSNQFLYSLVTTLW
                     YGVWGAVFSIFGLASALLVKKKLGAIFIPVAYMMVGGILWAILGLSYLEPVTTLALGY
                     QKDISLSLVSGHLAFILFVSCLVVYGTFFLHSEDYV"
     gene            1076..1828
                     /locus_tag="VYN69_001742"
     CDS             1076..1828
                     /locus_tag="VYN69_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533970.1"
                     /translation="MKQFVQFYKKDFLAVLVYFILLLSCVLSSTVYLLRCRQYSIHPN
                     VLEWILVLLQDMTTGVYCFPFTYILFFFYLMNNYFNRLECRIRLKSIKHFTSFSFKLA
                     ALSTGIWTAILFLLIFLIAFSNGFSFSLEIKEVDFLREFYGISIANNASFFIGFFFSY
                     IAYYFFLSLLTISSFSWFKKSNMSLVFLFTFLFVESLFWIYQLNNGIIGLLPIFQYMV
                     NSNPYALIYWLALLSIIIPLTVFSVHRNWRRV"
     gene            1838..2536
                     /locus_tag="VYN69_001743"
     CDS             1838..2536
                     /locus_tag="VYN69_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000517353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533971.1"
                     /translation="MGKLSSHIWRLNQIIYTKYFWGYVLFWILICLGLWYWLEGNDRL
                     VIEILKGPNLSQNSFLVLSIWLLHWFIIHTFFLAVVYRRRASDFFLEVIRFSSIKLWI
                     RYQIWTCFLYGLILIMVKVLVIQFMLQLPNWDIGVLFIVDFLNACVLVLFCFMLYALG
                     ANVQMNFACVSFFLLMIVFGGLFVGNRTNYLFYILNRGNGDIGRDLFLQLLFLVFLFK
                     SIFYFTRQKRRFIE"
     gene            2533..3180
                     /locus_tag="VYN69_001744"
     CDS             2533..3180
                     /locus_tag="VYN69_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1533972.1"
                     /translation="MNEIITLKNIELKLKKTCVFQNLNFSCKQGEIIGITGANGSGKS
                     VLFKLIAGLYSPSYGEVLINGENIVPERKIPANLGALIEEPGFINYYSGFKNLQYLAS
                     IRGVVGNQEINDTLKIVGLYEQKDQKVKTYSLGMRKKLGIAQAIMENPSILLLDEPMN
                     ALDKSSVENMRTLFRKLSSEKGTTILIASHSEEDIRILCDKVYAIEDKVCTLCSD"
     gene            complement(3268..4139)
                     /locus_tag="VYN69_001745"
                     /pseudo
     CDS             complement(3268..4139)
                     /locus_tag="VYN69_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine ABC transporter substrate-binding
                     protein"
     gene            complement(4151..4910)
                     /locus_tag="VYN69_001746"
                     /pseudo
     CDS             complement(4151..4910)
                     /locus_tag="VYN69_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012000401.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
     gene            complement(4911..5198)
                     /locus_tag="VYN69_001747"
     CDS             complement(4911..5198)
                     /locus_tag="VYN69_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1533973.1"
                     /translation="MTNVQLYYHIIIPQVLRRLLPQAINLVTRMIKTTSLVVLIGVVE
                     VTKVGQQILDSNRLTIPTASFWIYGTILVLYFAVCYPISKLSTHLEKHWRN"
     gene            complement(5208..5588)
                     /locus_tag="VYN69_001748"
     CDS             complement(5208..5588)
                     /locus_tag="VYN69_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154138.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEV1533974.1"
                     /translation="MQDSGIQVLFQGNNLLRILQGLGVTIGISILSVLLSMMFGTVMG
                     IIMTSHSRIIRFLTRLYLEFIRIMPQLVLLFIVYFGLARNFNINISGETSAIIIFTLW
                     GTAEMGDLVRGAITSLPKHQFESG"
     gene            complement(5548..6228)
                     /locus_tag="VYN69_001749"
     CDS             complement(5548..6228)
                     /locus_tag="VYN69_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000384089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1533975.1"
                     /translation="MDWSIVEQYLPLYQKAFFLTLHIAVWGILGSFLLGLIVSIIRHY
                     RIPVLAQVATAYIELSRNTPLLIQLFFLYFGLPRIGIVLSSEVCATLGLVFLGGSYMA
                     ESFRSGLEAISQTQQEIGLAIGLTPLQVFRYVVLPQATAVALPSFSANVIFLIKETSV
                     FSAVALADLMYVAKDLIGLYYETDIALAMLVVAYLIMLLPISLVFSWIERRTRHAGFG
                     NPSTLSRK"
BASE COUNT         1809 a         1027 c         1314 g         2131 t
ORIGIN      
        1 ggcggtaaaa ggttaaatac ctctattcgt agcgcctata gtgggtcaga tattaccccg
       61 gtatattcat tggggtctgg ctctaggatt gtcatgtact ataatggagg tggtgacaat
      121 tatattggtt ctggtactag attagctatg gcgccacaat ttggaaatca tgtaagaatt
      181 catacttcag gttcttggag tccagattct tattaactta cttgtcagag taagccttaa
      241 agatggttga ttgtgggtgt agcatgaaaa aagaatgcta caccctattt ttattataag
      301 gaggagtaag gatggaattt ttcatttgta atcttgtacg agtcgttcaa tcacctcggt
      361 tttatatgtc tttatttttg acccttcttt gcatgagttt aggaaatttt cttgctttca
      421 atggtattta taaaattgaa ggtttatcga ttttttttgc cgcttcttct attcgaggat
      481 tttcaccgat tagcctagta gctgcactta tctgtacact gccctattct agtcagataa
      541 tagaggatgc tgagagtcat tttctaacag cacaattgtg ccgaatttct aaaaagaggt
      601 atctagctat tgtaggtagt actgcaatta tttcttcttt tctagtcttt tttctcccct
      661 atttattatt attaggaatt aatcttttag tgactcctta tcaggaaatt tatattggag
      721 attatagtgg tgccttaaaa gaattatttg attctaatca gtttctctat agtcttgtga
      781 cgactctctg gtatggagtt tggggcgctg tgttctctat ttttggacta gctagtgctt
      841 tgctagtgaa gaaaaaatta ggagctattt tcatcccagt tgcctatatg atggttggtg
      901 gtattttatg ggctatttta gggctatctt acttagaacc tgtgacaacg ctagctttgg
      961 gatatcagaa agatatcagt ctttccttag ttagtgggca tcttgctttt attttatttg
     1021 ttagttgttt ggttgtttat ggtacatttt ttctacattc agaggactat gtataatgaa
     1081 acaatttgtt caattttata aaaaagattt cttagcagta ttggtttatt ttatattact
     1141 gctatcctgt gttttatcta gtacagtata tttattgcgc tgtcgccaat attcaatcca
     1201 tccaaatgta ttagaatgga tcttagtttt acttcaagat atgacgactg gagtatattg
     1261 ctttccgttc acatatatat tgttcttttt ttatttgatg aataactatt ttaataggtt
     1321 ggagtgtcgc attcgtctga aatcaattaa gcactttacc agttttagtt tcaaattagc
     1381 agctcttagt acggggattt ggacggcgat tttattttta ttgatttttc taattgcatt
     1441 tagtaatggt tttagcttct ctttggagat aaaggaggtt gattttttaa gagaatttta
     1501 tggcataagt attgcaaaca atgctagttt ctttatagga ttttttttct cttatatagc
     1561 atactatttc tttttatcct tacttactat tagcagtttt tcttggttta aaaaatcaaa
     1621 catgagctta gtatttctgt ttactttttt atttgtagaa tccttattct ggatttatca
     1681 gttgaacaat gggataattg gattattgcc aatttttcag tatatggtaa attccaatcc
     1741 gtatgcattg atttattggc ttgcattact atctatcata attccattga ctgtattttc
     1801 tgttcataga aactggagga gagtgtaaaa gttggaaatg ggaaagttaa gtagtcacat
     1861 atggaggttg aatcagataa tctataccaa gtacttttgg ggttatgttc ttttttggat
     1921 attgatttgt ttaggattat ggtattggtt agaaggaaat gatagacttg ttatagaaat
     1981 tttaaaaggg cctaatctga gtcaaaactc ttttttagtc ttatctatat ggttgcttca
     2041 ttggtttatt attcatacat tttttctagc agttgtatat cgtagaagag catccgattt
     2101 ctttctggaa gtgattcgat tttcttctat taagctctgg attaggtatc agatttggac
     2161 ctgttttctt tatggactca ttttaatcat ggtaaaagtt ctagtgattc aatttatgtt
     2221 acagttacca aactgggata taggagtttt gtttatagtt gattttttga atgcttgtgt
     2281 gttagtcttg ttttgcttta tgttatacgc actaggagcg aatgtacaaa tgaactttgc
     2341 ttgcgttagt ttctttttac tcatgattgt gtttggtggt ctatttgtag gaaatcgaac
     2401 taattatcta ttttatattt taaatcgagg gaatggagat ataggacgag atttatttct
     2461 tcagttgcta tttttagtct ttctttttaa aagcattttc tatttcactc gtcaaaagag
     2521 gaggtttata gaatgaatga aataattaca ttaaaaaata ttgagttgaa attaaaaaaa
     2581 acatgtgttt ttcaaaacct taattttagt tgtaaacagg gggaaattat aggaattact
     2641 ggtgcgaatg gctcagggaa aagtgtattg tttaaattaa tagctggttt atatagtccg
     2701 tcttatggag aagtgttaat caatggggaa aatattgttc ctgagagaaa aattccagct
     2761 aatttgggag ctttgattga agaacctggt tttataaatt attatagtgg ctttaagaat
     2821 ttacaatatt tggcaagcat acgaggagta gttggtaatc aggaaatcaa tgatacactg
     2881 aaaatagttg gtctatatga gcaaaaagac cagaaagtta aaacttattc gctaggtatg
     2941 aggaaaaagc tagggattgc tcaagcaatt atggagaatc cctctattct tttactagat
     3001 gaacctatga atgccttgga taaatcaagt gtagaaaata tgagaacatt gtttagaaag
     3061 ctctctagtg aaaaagggac aacaattttg attgctagtc atagtgaaga ggatattcgt
     3121 atcttatgtg ataaagtata tgcaatagaa gataaagtat gtacactgtg ttcagattga
     3181 acatcttaga tagactcagg atgttatagg taaaatctta aaaagggatt ggattttaaa
     3241 atccagttcc tttttaagag ttaatgacta atcaactttt ccaccttcaa caaccaggtc
     3301 atctgcttta gcagcgtcac cgtaggttgg gtgaagtgtt ttttcatagg ccttgtggaa
     3361 gaagttttcc ttgcctaatt tttcaatatc tttattgatg aagtctagca attcttggtt
     3421 gcctttttga actgctgccg caatggtatc gggatcaccg agggaagtaa ttcctacttc
     3481 aaatccttta ttttcaagcg cccaagctag aacttccgta ttgtcagttg aaaaggcatc
     3541 tccacgtccg tcaagaagag cttggtaaga gtcactgtat tggtcgtatt tttggagttt
     3601 gatttctgga tgattctttt caaaataagt ctcagcagtc gttccttttg tgacaattaa
     3661 ggttttacct tcaagttgtt tgacgtctgt aatgagacca gtcttaggtg atacgacacc
     3721 cagagaaact ttcatatatg gaagggcaaa atcaacttgt ttcttacgtt cgtcagttac
     3781 tgtaaagtta gcaagagtaa tatctacctt gtttgaaatc aagtattccg cacggttggc
     3841 agcatcgact gaaatgtatt taaccttgac accaaggtct tgagctagtt ggttccctag
     3901 ttcaatatcg tagcgtagcc ttggtaagaa ccatcattgt caacgtagcc aaacggtttt
     3961 ttatctccaa acacggcgat tcgcagttca ccgctttttt tgatttcatc gatagtgcga
     4021 gctttggcag ttgtttttcc agaggatgaa ccagcgtttc cacctgagct acaagcagtg
     4081 ataaggataa gggcaaaggc aagtgctaaa acagttaaga gtggtttgaa tagtttcata
     4141 agaatctcct ttatagatat gagccgaatt ggctaaagtc aaagacgttt aaaaattcct
     4201 gggctcgttt ggtttgcgga ttggtaaaga aggcttgagc tgttccttct tcagcgattt
     4261 tcccttggtc gaggaagata atccggtcag taatggcttg ggcaaactgc atttcgtggg
     4321 ttactaaaat catggtacgg ccttcttggg tcaaatcatt gataagttcc agcacctcac
     4381 gcaccatttc tggatccagc gaagcagtca cctcgtcaaa aaggatgatt tctggatgca
     4441 ttaggagggc acggacaatt gcaacacgtt gcttctgtcc accagataat tgacgggcaa
     4501 agctatgttg tttatccagc aaaccgacac gctctagtaa ttgcaaagct tcttccgtta
     4561 cttctttctt gtcccttcct tgagctttga tagggcctag gatgaggttt tgtaagacat
     4621 ccagatgggg aaagagttca taactttgaa agaccatgcc aatcttttgg cgaactaggt
     4681 gaaaatcttt tttattttca acgatagact gaccatccag aagaatatct ccaccttgaa
     4741 tactttctaa gccgttgagg caacgaagga gggtactttt cccacaacca gatgccccta
     4801 ggatgacaac aacttccccc ttttttgatt tctagagaaa gtccttggag gatgggattg
     4861 tctccgaagg atttttttag ttccttgatt tctaagatag tttcagacat ttagtttctc
     4921 caatgttttt ctaagtgagt ggatagtttg gaaatagggt agcaaactgc gaaatataag
     4981 actagaatgg ttccataaat ccaaaatgaa gcagttggga tggtcaggcg attgctatcg
     5041 aggatttgtt gtccaacttt ggtcacttcc acaaccccaa tcaaaacaac taatgaagtg
     5101 gttttaatca tccgagtgac aagattgata gcctgcggta gcagtcttct taagacttgt
     5161 gggatgatga tgtggtagta aagttgaaca ttagtcaagt cgagtgccta tccactttca
     5221 aactgatgtt tagggagaga agtgatagct ccacgtacca agtctcccat ttcagctgtt
     5281 ccccagaggg taaaaatgat aatagctgaa gtctcacctg agatattgat attaaagttt
     5341 cgagccaagc caaagtaaac gatgaagagt agcaccagct ggggcatgat acggataaat
     5401 tccagataca atcgtgttaa aaatcgtatg attctagaat gggaggtcat gatgattccc
     5461 atgactgttc cgaacatcat ggataagagg acagacagga tggatatccc aatcgtaacg
     5521 cccaacccct gtaagattct caggagatta tttccttgaa agagtacttg gattcccgaa
     5581 tcctgcatgg cgggtcctcc tttctatcca gctaaagacc agtgagatgg gtagcagcat
     5641 gatcagataa gcaactacca acatagctag cgcaatgtct gtctcatagt agagaccaat
     5701 caaatccttg gcgacgtaca tgaggtcggc caaagccact gctgagaaaa cagaggtttc
     5761 cttgataagg aaaatgacat tggcactaaa ggaggggagt gccaccgctg ttgcttgcgg
     5821 aagaaccaca tagcgaaaga cctgtagagg tgtcagacca atagcgaggc caatctcctg
     5881 ctgggtttga ctgatggctt ccagcccact tcggaaagat tctgccatat aggagcctcc
     5941 taaaaagaca aggccaagcg ttgcacagac ttctgaagat aggacaatcc cgattcgggg
     6001 aagaccgaag tagagaaaga agagttgaat caaaaggggc gtattacgtg acaattcaat
     6061 gtaggctgtc gctacttgcg ccaaaacagg gattcgataa tgtcggatga tactaacgat
     6121 taaaccgagc agaaaggatc ccaaaattcc ccaaactgca atatgcaagg tcagaaagaa
     6181 tgccttttga tatagtggta gatattgttc aacaatggac caatccaaaa atagaacctc
     6241 ccatctagaa ataatacagt tattgtagca cttaaaatct t
//
LOCUS       DAVLVP010000095         6123 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.095, whole genome shotgun sequence.
ACCESSION   DAVLVP010000095 DAVLVP010000000
VERSION     DAVLVP010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 6123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6123
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.095"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(69..344)
                     /locus_tag="VYN69_001750"
     CDS             complement(69..344)
                     /locus_tag="VYN69_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEV1533976.1"
                     /translation="MANKQDLIAKVAEATELTKKDSAAAVEAVFAAVADYLAAGEKVQ
                     LIGFGNFEVRERAERKGRNPQTGKEMTIAASKVPAFKAGKALKDAVK"
     gene            complement(450..1289)
                     /locus_tag="VYN69_001751"
     CDS             complement(450..1289)
                     /locus_tag="VYN69_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEV1533977.1"
                     /translation="MTKIKIVTDSSVTIEPELVKQLDITIVPLSVMIDNVVYSDADLK
                     EEGKFLQLMQESKNLPKTSQPPVGVFAEIFEDLCKDGGQILAIHMSHALSGTVEAARQ
                     GASLSTADVTVVDSSFTDQALKFQVVEAAKLAQEGKDMEAILSHVEEVKNHTELYIGV
                     STLENLVKGGRIGRVTGLLSSLLNIRVVMQMKDHELQPMVKGRGTKTFKKWLDELITS
                     LSERAVAEIGISYSGSDDWAKEMKESLQAYVEKPISVLETGSIIQTHTGENAWAILIR
                     YHS"
     gene            1431..2279
                     /locus_tag="VYN69_001752"
     CDS             1431..2279
                     /locus_tag="VYN69_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEV1533978.1"
                     /translation="MIKKIYPIFTILLGAAIYAFGLTYFVVPHHLFEGGATGITLITF
                     YLFKIPVSLMNLLINIPLFILAWKIFGAKSLYSSLLGTLALSAWLAFFEHIPLHIDLQ
                     GDLLITALIAGILLGIGLGIIFNAGGTTGGTDILARILNKYTHISIGKLLFILDFCIL
                     MLILLIFKDLRLVSYTLLFDFIVSRVIDLIGEGGYAGKGFMIITKRPDQLAKAINDDL
                     GRGVTFISGQGYYSKENLKIIYCIVGRNEIVKTKEMIHRIDPQAFITITEAHEILGEG
                     FTFEKE"
     gene            complement(2372..3289)
                     /locus_tag="VYN69_001753"
     CDS             complement(2372..3289)
                     /locus_tag="VYN69_001753"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HEV1533979.1"
                     /translation="MIITIPIKNQKDIGTPSDSVVVLGYFDGIHKGHQELFRVANKAA
                     RKDLLPIVVMTFNESPKIALEPYHPDLFLHILNPAERERKLKREGVEELYLLDFSSQF
                     ASLTAQEFFATYIKAMNAKIIVAGFDYTFGSDKKTAEDLKDYFDGEVIIVPPVEDEKG
                     KISSTRIRQAILDGNVKEAGKLLGAPLPSRGMVVHGNARGRTIGYPTANLVLLDRTYM
                     PADGVYVVDVEIQRQKYRAMASVGKNVTFDGEEARFEVNIFDFNQDIYGETVMVYWLD
                     RIRDMTKFDSVDQLVDQLKADEEVTRNWY"
     gene            3727..3932
                     /locus_tag="VYN69_001754"
                     /pseudo
     CDS             3727..3932
                     /locus_tag="VYN69_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001844775.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(4243..4536)
                     /gene="rpmA"
                     /locus_tag="VYN69_001755"
     CDS             complement(4243..4536)
                     /gene="rpmA"
                     /locus_tag="VYN69_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEV1533980.1"
                     /translation="MLKMTLNNLQLFAHKKGGGSTSNGRDSQAKRLGAKAADGQTVTG
                     GSILYRQRGTHIYPGVNVGRGGDDTLFAKVEGVVRFERKGRDKKQVSVYPIAK"
     gene            complement(4553..4897)
                     /locus_tag="VYN69_001756"
     CDS             complement(4553..4897)
                     /locus_tag="VYN69_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000613691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEV1533981.1"
                     /translation="MIQAVFERAEDGELRSAEITGHAESGEYGLDVVCASVSTLAINF
                     INSIEKFAGYEPILELNEDEGGYLMVEIPKDLPSHQREMTQLFFESFFLGMANLSENS
                     SEFVQTRVITEN"
     gene            complement(4913..5227)
                     /gene="rplU"
                     /locus_tag="VYN69_001757"
     CDS             complement(4913..5227)
                     /gene="rplU"
                     /locus_tag="VYN69_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018374562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEV1533982.1"
                     /translation="MSTYAIIKTGGKQVKVEVGQAVYVEKLNVEAGQEVTFNEVVLVG
                     GENTVVGTPLVAGATVVGTVEKQGKQKKVVTYKYKPKKGSHRKQGHRQPYTKVVINAI
                     NA"
     misc_feature    complement(5263..5350)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            complement(5415..6113)
                     /locus_tag="VYN69_001758"
     CDS             complement(5415..6113)
                     /locus_tag="VYN69_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004241810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEV1533983.1"
                     /translation="MGGKMRLLPIRKISRQSKRLALFLTFCAGYVDAYTFIVRGNTLV
                     AGQTGNVVFLSVELIKNNVSDVRDKVLTLLAFMMGVFLLTIYKEKLRIVKKPILSLIP
                     LAILSIIIAFVPQTVDNIYLVPPLAFCMGLVTTAFGEVSGIAYNNAFMTGNIKRTMLA
                     FGDYFRTKHTPFLREGFIFVSLLSSFVLGVVFSAYLTIFYHEKTILGVPIMMSVFYLS
                     MLFASWQKKVKEKA"
BASE COUNT         1877 a         1259 c         1032 g         1955 t
ORIGIN      
        1 gatttcataa ttaaaaagcc tactgaccaa gctagaaagc ttgatacaat aggcttttta
       61 aagactgatt atttaacagc gtctttaaga gctttaccag ctttgaatgc tggtacttta
      121 gaagctgcaa ttgtcatttc tttaccagtt tgtgggttgc gacctttacg ttctgcgcgc
      181 tcacgaactt caaagttacc aaaaccgatc aattgaactt tttcaccagc tgcaagatag
      241 tcagctactg ctgcaaatac agcttcaact gctgctgctg agtctttctt agtcaattct
      301 gtagcttctg ctactttagc gatcaaatct tgtttgtttg ccatgttaat gattcctcca
      361 aataatttct aattaacaaa tataatcata tcctaaaatc ccttttaggt caagttaaaa
      421 acgctatttc ttcccatttt atttattttt taggagtggt aacgtatcaa aatagcccaa
      481 gcgttctcac ccgtgtgagt ttgaataatg gaacccgttt ccaaaacaga aattggcttt
      541 tcaacataag cttgtaagct ttctttcatc tcttttgccc aatcatcact accagaatat
      601 gaaattccaa tctctgctac agcacgttca gaaagcgatg ttatcaactc atctaaccat
      661 tttttaaatg ttttagttcc acgaccttta accattggct gcaattcatg gtctttcatt
      721 tgcatgacaa cacggatatt gagaagggag ctcaacaagc cagttacacg gccaattcgt
      781 ccacctttga caagattttc caaagttgaa acaccaatat aaagctctgt atggttttta
      841 acctcttcta catgagataa aattgcctcc atatctttac cttcttgagc taacttcgca
      901 gcctcaacaa cttggaattt cagggcttgg tcagtgaagg aactatcaac aacagtcaca
      961 tctgcagtag ataggctagc accttggcgt gctgcttcta ccgtacccga aagagcatgg
     1021 gacatatgaa tagcaagaat ctggccacca tctttgcata ggtcttcaaa aatctcagca
     1081 aagacaccta caggtggctg acttgttttc ggaagattct tactttcttg catcaactga
     1141 agaaatttac cttcttcttt caaatccgca tcagaataaa caacattatc aatcattaca
     1201 gataatggaa caattgtaat atctaattgc tttactagtt caggttcaat agtaacagat
     1261 gaatcggtta caatcttaat ttttgtcata gtatcaatct ttctatttta ggattcagat
     1321 tggtttcctt acttctaatt atatcaaaaa aaagattaaa aatcctaatg gagtcaatca
     1381 aattttccgt aaaatttgat ataatcaact tataagaaaa gaggtgtcct atgattaaaa
     1441 aaatttaccc catttttacc attttactag gtgctgctat ttatgctttt ggactgactt
     1501 attttgtagt tccccatcat ctctttgaag gaggggcgac aggcattacc ctcatcacct
     1561 tttatctttt taaaatccct gtttccctca tgaacctgct gattaatatt ccccttttca
     1621 tcctagcttg gaagatcttt ggagccaaat ccctctattc tagtttacta ggaaccttag
     1681 ctttgtccgc ctggttagct ttttttgagc atattcccct tcatattgat cttcaaggtg
     1741 atttactaat cacagccctt atagcgggaa tcctattggg aattggcctt ggaattattt
     1801 ttaatgctgg aggtacaact ggcggaactg atattctagc tcgtattctc aacaaataca
     1861 ctcatatatc cataggaaaa ctgctcttta tcttagattt ttgtattctc atgttgattc
     1921 tcctaatctt caaggatttg agattggttt cctacacgct tttgtttgat tttattgttt
     1981 ctcgtgttat tgatttgatt ggtgaaggag gatatgccgg caaaggcttt atgattatca
     2041 caaaacgtcc tgaccaactt gctaaggcga ttaatgatga cctcggaaga ggtgttactt
     2101 ttatttctgg tcaaggctac tatagtaaag aaaatttgaa aatcatctac tgtattgtcg
     2161 gaagaaatga aattgtgaaa acgaaggaaa tgattcatcg aatcgatcct caagccttta
     2221 taactattac agaagcccat gaaatcctag gagaaggctt cacctttgaa aaagaataaa
     2281 aagaggtaat gtcgtgacct caaaagttag actaaatcat ctatcttttg ggttacagac
     2341 aacctctttt ttattttatt tactcaagct cttaatacca attccgagtt acttcttcat
     2401 cagcctttaa ctgatccact aattggtcaa ctgagtcaaa tttggtcata tctcgaatgc
     2461 gatcaagcca ataaaccatg acggtttccc cataaatatc ttgattaaaa tcaaaaatat
     2521 tgacttcaaa acgtgcttct tctccatcaa aggtcacatt tttcccgaca ctagccatag
     2581 cacgatactt ctgtctttga atctcaacat caacaacata aacgccatct gctggcatat
     2641 aagtacggtc taaaagcact aaattcgctg tcggataacc aattgtacga ccacgagcat
     2701 taccatgaac caccatacct cttgatggaa gcggtgcccc caaaagtttt cctgcttctt
     2761 tcacatttcc atctaaaata gcttgacgga tacgagttga actaatcttt cctttctcat
     2821 cttctacagg tggaacaatg ataacttctc catcaaagta atcctttaaa tcttctgctg
     2881 tttttttgtc agaaccaaat gtataatcaa aacctgcaac aataattttg gcattcatag
     2941 ccttgatata agttgcaaag aattcttgtg cagtgagact agcgaattga ctactaaaat
     3001 caaggagata taattcttct acaccttcgc gctttaattt tctttcacgt tcagcagggt
     3061 tcaaaatatg caaaaacaaa tctggatgat aaggctctaa agcgatcttt ggagattcat
     3121 taaaggtcat aacgacgata ggcaacaaat cctttctcgc agccttgttg gcaacacgaa
     3181 ataattcttg atgcccctta tgtatgccat caaaatagcc gagaacaacg actgaatcag
     3241 atggtgtgcc aatatctttt tggtttttta taggaatagt aataatcata aaataattat
     3301 atcatagcga tagctatttc tggaacagaa aatctgaaat gttgtttttt tcacatgaag
     3361 tgtacctgtt ttcaaaaagc actttattct atcgttgctt aactatgaac tttgcaatat
     3421 tcttctcaaa aacttgtagg acatcttcaa aattttgcaa ggagtgatta gacttgttcg
     3481 gtaactataa agtgtcatac tatgcttatg tatgaaaaag caatgcaact aactcctgag
     3541 aactttaaat tactaattgg tgccgaaaag gtagaattta gaatagaggt acacctatgg
     3601 ctgtaaaatt tacaaaatga gacaacttgg gcaagatgtt tgaagaattt cctaaactcc
     3661 ctgatttgaa gcaagtcact ttccctgatg acaaagaaaa aagccaaagc aataaagaaa
     3721 aactagatga ctgctttcca acaactccca tctagtgtgc ttcagactgg gctatttttc
     3781 tctccatctg ttagcttgga ttctcagacc gtttcagcta aagaatatct tttcctttat
     3841 cagaaggaac ggctcaagcc attcagacaa gtgaagggac gacaagccaa tatttgaaac
     3901 cagatagcag ttcttatagt caattgaaat aaaatctgaa gaaatcgagt aggaaactca
     3961 aatcaatttt tagcagtgtt ctattccaga ttcatactca atgaaaatta aagtgcaaac
     4021 taggaagtta gccgcaggct gtacttgagt acggcaaggt gaaactgacg tggtttgaat
     4081 ttgattttcg aagagtatca atccactata catattaaaa agtatgggta tcaaaataaa
     4141 aaaacaggct ctccgaaaac tcggaaagcc ttattttaag ctgtttctag tttcctcacc
     4201 tttacatttc aaggttcggg ataaaaggtt caatggacct ttttatttag cgattgggta
     4261 aacagaaact tgttttttat cgcgtccttt acgttcaaag cgtactacgc cttcaacttt
     4321 agcgaacaaa gtatcgtctc caccacgacc aacgtttaca cctggataga tgtgtgtacc
     4381 acgttgacgg taaaggattg atccacctgt tacagtttgt ccgtcagctg ctttagctcc
     4441 aagacgtttt gcttgtgaat cacgtccgtt tgatgtagaa cctccacctt ttttgtgggc
     4501 gaaaagttgc aagttgttaa gagtcatttt taacataatg ttttcctccg tgttagtttt
     4561 ctgtgataac tctggtttgg acgaactcag aagagttctc cgataagttt gccataccta
     4621 agaaaaatga ttcaaagaat aactgggtca tttctctctg gtgtgaagga agatcttttg
     4681 gtatttcaac catcagatag ccaccttcat cttcgtttaa ttctaggatt ggttcatagc
     4741 ctgcaaattt ctcaatagaa ttgataaagt taatggcaag cgtagaaacc gatgcacaca
     4801 cgacatctaa gccgtattcg ccactctcgg cgtgtccagt aatttccgca ctcctcagct
     4861 cgccatcttc ggctctctca aagactgcct gtatcatgtg ttctccttaa aattaagcgt
     4921 tgattgcgtt gatgacaact tttgtatatg gttgacggtg accttgttta cggtggctac
     4981 cttttttagg tttatacttg taagtaacca ctttcttttg ttttccttgt ttttcaacag
     5041 ttccaactac agtagctcca gcaacaagtg gagttccgac aacagtgttt tcaccaccaa
     5101 caagaacaac ttcgttaaaa gtaacttctt gaccagcttc aacgttcaat ttttcaacgt
     5161 aaactgcttg accaacttca actttaactt gttttccgcc agttttgata attgcgtatg
     5221 tgctcattat gcacctccta tgatttttat gggtttcccc gattttttgt gaagactcgc
     5281 ctagcatcgt gggacgaacc acttaaactt gaataatcaa gcaacgatgt tcgtgcggtt
     5341 gcacagggtc gtgcatagtc aactcttcaa gtatagcata tctcctattt tcttacaagt
     5401 aataacacct aaaatcaagc tttttctttt acttttttct gccaagaggc aaaaagcatg
     5461 ctgaggtaaa aaacgctcat cataatagga acaccaagaa tggtcttttc atgatagaaa
     5521 atcgtcaaat aggctgaaaa gacaacgcca aggacaaaac tactaagcag gctaacaaat
     5581 atgaatcctt cacgcaaaaa aggagtgtgc ttggttcgga aataatctcc aaaagccagc
     5641 atggtccgtt tgatattccc tgtcataaaa gcgttattat aggcaatacc cgacacttct
     5701 ccaaaagcag ttgtcaccag tcccatacag aaggccaagg gcggcactag atagatatta
     5761 tccacagttt gcggcacaaa agcaataatg attgataaga ttgccaaggg aatcaaggac
     5821 agaataggtt ttttcacaat tctcaatttt tccttataaa tcgttaataa aaagactccc
     5881 atcataaacg ctagcaaggt gagaaccttg tccctaacat ccgaaacatt atttttaatt
     5941 aattctactg aaagaaagac aacatttcca gtttgtccag ctacaagggt attcccgcga
     6001 acaataaaag tgtaagcatc cacatatcca gcacaaaacg tcaaaaaaag tgctaacctt
     6061 ttagactgac gtgatatttt tcttataggt aataacctca ttttacctcc cattgtattt
     6121 tct
//
LOCUS       DAVLVP010000096         5749 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.096, whole genome shotgun sequence.
ACCESSION   DAVLVP010000096 DAVLVP010000000
VERSION     DAVLVP010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5749)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5749)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5749
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.096"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(213..1883)
                     /locus_tag="VYN69_001759"
     CDS             complement(213..1883)
                     /locus_tag="VYN69_001759"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019803354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEV1533984.1"
                     /translation="MKTDIEIAQSIELKPIVDVVEKLGISYDDLELYGKYKAKLSFDK
                     IRAVESNPVGKLILVTAINPTPAGEGKSTLTIGLADALNKIGKKTMIAIREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALIDNHLHQGNELGIDQRR
                     ILWKRVVDLNDRALRHVTVGLGGPLNGIPREDGFDITVASEIMAILCLATDIEDLKRR
                     LANIVIGYRYDRTPVSVGDLQVEGALALILKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSVLATTTALHLADYTVTEAGFGADLGAEKFLDIKTPNLPTSPDAVVIVATLRAL
                     KMNGGVAKDALTEENVEAVRAGFANLKRHVENIRKFGIPAVVAINEFVSDTEAEIAAL
                     KELCASIDVPVELASVWADGAEGGVALAETVVKTIAENPANYKRLYDNDLSVQEKIEK
                     IVTEIYRGSKVNFEKKAQTQIAQIVQNGWDKLPICMAKTQYSFSDNPNALGAPENFEI
                     TIRELVPKLGAGFIVALTGDVMTMPGLPKRPAALNMDVESDGTVLGLF"
     gene            2113..2802
                     /gene="coaB"
                     /locus_tag="VYN69_001760"
     CDS             2113..2802
                     /gene="coaB"
                     /locus_tag="VYN69_001760"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000699493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEV1533985.1"
                     /translation="MKILVTSGGTSEAIDSVRSITNHSTGHLGKIITETLLSAGHEVC
                     LITTKRALKPEPHPNLSIREITNTKDLLIEMQERVQDYQVLIHSMAVSDYTPVYMTGL
                     EEVQASSNLKEFLSKQNHQAKISSTDEVQVLFLKKTPKIISLVKEWNPTIHLIGFKLL
                     VDVTEDHLVDIARKSLIKNQADLIIANDLTQISAGQHRAIFVEKNQLQTVQTKEEIAE
                     LLLEKIQAYHS"
     gene            2814..3365
                     /gene="coaC"
                     /locus_tag="VYN69_001761"
     CDS             2814..3365
                     /gene="coaC"
                     /locus_tag="VYN69_001761"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEV1533986.1"
                     /translation="MANILLAVTGSIASYKSADLVSSLKKQGHQVTVLMTQAATEFIQ
                     PLTLQVLSQNPVHLDVMKEPYPDQVNHIELGKKADLFIVVPATANTIAKLAHGFADNM
                     VTSTALALPSHIPKLIAPAMNTKMYDHPVTQNNLKTLETYGYQLIAPKESLLACGDHG
                     RGALADLTIILERIKETIDEKTL"
     gene            3349..3912
                     /locus_tag="VYN69_001762"
     CDS             3349..3912
                     /locus_tag="VYN69_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000747407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1533987.1"
                     /translation="MKKRSNIAPIAIFFATMLVIHFLSSLIFNLFPFPIKPTIVHIPV
                     IIASIIYGPRVGVTLGFLMGLLSLTVNTITILPTSYLFSPFVPNGNIYSAIIAIVPRI
                     LIGLTPYLVYKLMKNKTGLILAGALGSLTNTIFVLGGIFFLFGNVYNGNIQLLLATVI
                     STNSIAELVISAILTLAIVPRLQTLKK"
     gene            4194..4721
                     /locus_tag="VYN69_001763"
     CDS             4194..4721
                     /locus_tag="VYN69_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEV1533988.1"
                     /translation="MNTRKKTQFMTMTALLTAIAILIPIVMPFKIVIPPASYTLGSHI
                     AIFIAMFLSPLMAVFVILASSFGFLMAGYPMVIVFRAFSHISFGALGALYLQKFPDTL
                     DKPKSSWVFNFVLAVVHALAEVLACVLFYATSGTNVENMFYVLFVLVGFGTIIHSMVD
                     YTLALAVYKVLRKRR"
     gene            4733..5248
                     /locus_tag="VYN69_001764"
     CDS             4733..5248
                     /locus_tag="VYN69_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007522046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEV1533989.1"
                     /translation="MTKDRKQALLQLLKEAPKALNGQSLAEHFHVTRQVIVQDIAILR
                     ADGAPILSTNRGYIYKQIDTNPYVHKLFKVKHEVEEIGQELLAIVDNGGHVQNTLIDH
                     PVYGEIETLLKLSCRRDVQHFLEQVEHSDFRPLSELTDGIHYHLVEAETQQDLHYIEE
                     ALDQLGYLVKD"
     gene            complement(5245..5712)
                     /locus_tag="VYN69_001765"
     CDS             complement(5245..5712)
                     /locus_tag="VYN69_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000085289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase"
                     /protein_id="HEV1533990.1"
                     /translation="MSRSQLTILTNICLIEDLETQRVVMQYRAPENNRWSGYAFPGGH
                     VENDEAFAESVIREIYEETGLTIQNPQLVGIKNWPLDTGGRYIVICYKATEFSGTLQS
                     SEEGEVSWVQKDQIPNLNLAYDMLPLMEMMEAPDKSEFFYPRRTEDDWEKKIF"
BASE COUNT         1697 a         1258 c         1058 g         1736 t
ORIGIN      
        1 taggaagcta gccgcaggtt gctcaaaaca ctgttttgag gttgtggata gaactgacag
       61 agtcagtatc atattaccta cggcaaggtg aagctgacgt agtttgaaaa gattttcgaa
      121 gagtataaat cttattgatg aactgcttgc agtctgagaa aaaatgagct tggatattat
      181 ttccaaactc acttaaagtc aatttcaatc cactagaaca agcctagtac agttccatcg
      241 ctttcaacat ccatgttgag agctgctgga cgttttggaa gacctggcat ggtcataaca
      301 tcaccagtta aggcaacgat gaagcctgca cctaattttg gtaccaattc acgaatggta
      361 atttcaaagt tttctggtgc tccaagcgca tttggattgt ctgagaaact gtattgagtt
      421 ttagccatac aaattggcaa tttgtcccaa ccgttttgaa cgatttgagc aatttgtgtt
      481 tgagctttct tctcaaagtt cactttgcta ccacgataga tttcagtgac aattttttca
      541 atcttttctt ggacagaaag gtcattatcg tacaaacgtt tatagttagc tggattttca
      601 gcaattgtct taacaactgt ttcggcaagt gctactccac cttctgctcc atcagcccag
      661 acactagcca attcaactgg tacatcgatt gaggcacaga gttcttttaa ggctgcaatt
      721 tcagcttctg tatcagatac aaattcgtta atagctacaa ctgctggaat accgaactta
      781 cggatatttt caacgtggcg tttcaagtta gcaaaacctg cacgaactgc ctctacattt
      841 tcttcagtca gagcgtcttt agccacacca ccattcatct taagggcacg aagggttgcg
      901 acaataacaa ctgcatctgg agatgttggc aagtttggtg tcttgatatc aaggaatttc
      961 tcagcaccaa ggtccgcacc aaaaccagct tcagtaacag tgtaatcagc caagtgaagg
     1021 gctgttgtcg tcgccaaaac agagttacag ccatgagcga tattggcaaa tggaccaccg
     1081 tgtacaaagg caggtgtacc gtaaattgtc tgaaccaagt ttggcttaat agcatccttc
     1141 aaaatcaaag ccaaggcacc ctcaacctgc aaatcaccta cagaaacagg cgtacggtca
     1201 tagcgataac caataacgat attcgccaaa cgacgtttca agtcctcgat gtccgttgcc
     1261 aagcaaagaa ttgccatgat ttctgaagca actgtaatat caaaaccgtc ctcacgtgga
     1321 ataccgttta gaggaccacc aagaccaaca gtcacatggc ggagcgcacg gtcgttcaag
     1381 tccacaacgc gtttccagag gatacgacgt tgatcaattc ccagctcatt cccttggtgc
     1441 aagtggttgt caatcaaggc agaaagggca ttgttggcag ttgtaatagc atgcatatct
     1501 ccagtaaagt ggaggttgat gtcttccatt ggcagaactt gtgcataccc accaccagca
     1561 gcaccaccct tgatccccat gactggacca agagacggtt cgcggatagc aatcatggtt
     1621 tttttgccaa tcttgttcaa ggcatccgca agaccaatgg taagcgtcga ctttccttca
     1681 cctgcaggtg ttgggttgat ggcagtaacc aagatcaatt taccgactgg attgctctca
     1741 actgcacgaa ttttatcaaa gctgagttta gccttgtact ttccgtacaa ctccaaatcg
     1801 tcgtaagaaa taccaagttt ctctacaaca tcaacaattg gcttcaactc aatactctgt
     1861 gcgatttcaa tatctgtttt cattcaaaat tcctctaacc tcttatatga taattcatta
     1921 tatcacaaaa caagattttt aacatcctaa aactctctaa acgttcgtaa atatctctgt
     1981 ttttaaggct tttagagtcc tttcttaaat tttatatggc tttatagttt gaaactatag
     2041 taaatcttcg tttttaccaa aaatttatca ctttcatttt acttaccgct tatttttgtg
     2101 tacaatagtg ctatgaaaat tttagttaca tcgggcggta ccagtgaagc tatcgatagc
     2161 gtccgctcta tcactaacca ttctacaggt cacttgggga aaattatcac agagactttg
     2221 ctttctgcag ggcatgaagt ttgtttaatt acgacaaaac gagctctgaa gccagagcct
     2281 catcctaacc taagtattcg agaaattacc aataccaagg accttctaat agaaatgcaa
     2341 gaacgtgttc aggattatca ggtcttgatc cactcaatgg ctgtttctga ctacactcct
     2401 gtttatatga cagggcttga ggaagttcag gctagctcca atctaaaaga atttttaagc
     2461 aagcagaatc atcaggccaa gatttcttca actgatgagg ttcaggtttt gttccttaaa
     2521 aagacaccca aaatcatatc cctagtcaag gaatggaatc ctactattca tctgattggt
     2581 ttcaaactgc tggttgatgt taccgaagat catctggttg acattgcacg aaaaagtctt
     2641 atcaagaatc aagcagattt aatcatcgcg aatgacctga ctcaaatttc agcaggtcag
     2701 caccgagcta tatttgttga gaaaaatcag cttcaaacag tccagactaa agaagaaatt
     2761 gcagaactcc tccttgaaaa aattcaagcc tatcattctt agaaaggaaa actatggcaa
     2821 acattctctt ggctgtaacg ggttcaatcg cctcttataa gtcggcagat ttagtcagtt
     2881 ctctaaaaaa acaaggccat caagtcactg tcttaatgac tcaggctgct acagagttta
     2941 tccaaccttt gacactacag gtactctcac agaatcctgt ccacttggat gtcatgaagg
     3001 aaccctatcc tgatcaggtc aatcatatcg aacttggaaa aaaagcagat ttatttatcg
     3061 tggtacctgc aactgctaac actattgcaa aactagctca cggatttgcg gacaacatgg
     3121 taaccagtac agctctagcc ctaccaagtc atattcccaa actaatagct cctgctatga
     3181 atacaaaaat gtatgaccat ccagtaactc agaataatct gaaaacatta gaaacctacg
     3241 gctatcagct gattgctcct aaggaatctc tactagcttg tggagaccac ggacgaggag
     3301 ctttagctga cctcacaatt attttagaaa gaataaagga aactatcgat gaaaaaacgc
     3361 tctaatattg cacccattgc tatctttttt gctaccatgc tcgtgataca ctttctgagc
     3421 tcacttatct ttaacctttt tccatttcca atcaaaccga ccattgttca tattcctgtc
     3481 attattgcca gcattattta tggtccacga gttggggtta cacttggatt tttgatggga
     3541 ttacttagct tgacggttaa cacgattacg attctaccga caagctacct cttctctccc
     3601 ttcgtaccaa acggaaacat ctactcagct atcattgcca tcgtcccacg tattttgatt
     3661 ggtttaactc cttacttagt ctataaactg atgaaaaaca agactggtct gattttagct
     3721 ggagcccttg gttccttgac aaatactatc tttgtccttg gaggaatctt cttcctattt
     3781 ggaaatgttt ataatggaaa tatccaactt cttctggcaa ccgttatctc aacaaattca
     3841 attgctgaat tggtcatttc tgcaattcta accctagcca ttgttccacg actacaaacc
     3901 ttgaaaaaat aaaaacaatc tccgctttca aacctgaagg tggagatttt gctctataca
     3961 gtttcttttt aatgaaatct ttactatagt aaaacgaaat aagaacagga caaatcgatc
     4021 aggacagtca aatcgatttc taacaatgtt ttagaagtag aggtggacta ttctagtttc
     4081 aatctactat aacttttaac taaaaaatga tctataaacc caagtaaatt cttgctttat
     4141 tgtctggttt attgtaccat actagtgtat atgcagttaa aaaggagatt cttgtgaata
     4201 cacggaaaaa gacacaattt atgacaatga cagccctttt aacggctatt gcgattttga
     4261 ttccaattgt tatgcctttc aagattgtca ttccacctgc ttcctatact ttggggagcc
     4321 acatcgctat ttttatagcc atgttcttgt cgcccttgat ggcagttttt gtcatcctag
     4381 cctctagttt tggatttttg atggctggct atcccatggt tatcgttttt cgggcttttt
     4441 cccatatatc ttttggtgct ttaggagctc tttacctaca aaaattcccc gataccctag
     4501 ataaaccaaa atcttcctgg gttttcaatt ttgttttggc tgttgttcat gcccttgctg
     4561 aagtattagc ctgtgtcctt ttttacgcaa cttcgggtac caatgtagaa aatatgttct
     4621 atgttctatt tgtactagtt ggatttggta caattatcca tagtatggta gactatacat
     4681 tagcactagc tgtctataaa gtgcttcgaa aacgccgtta aaaggaaaag ctatgacaaa
     4741 agatcgcaaa caagccctgc tccaactctt gaaagaagct cctaaagccc ttaatggcca
     4801 aagtttggcg gaacattttc atgtcacacg tcaggtcatt gtgcaggaca ttgcaatttt
     4861 gagagccgat ggcgctccta tcctatccac caatcgtggc tacatctata agcaaattga
     4921 taccaatcct tatgttcaca aacttttcaa agtgaaacat gaagttgaag aaatcggtca
     4981 ggaactcctt gctattgtcg ataatggtgg gcatgtccag aataccttga ttgaccatcc
     5041 cgtttatgga gaaattgaaa ccttgcttaa actgtcttgt cgcagagatg tgcaacattt
     5101 tctagaacaa gtcgagcatt ctgactttag acctctatct gaattgacag atggcatcca
     5161 ttaccaccta gtcgaagccg aaacacaaca agacctccac tatatcgagg aggccttgga
     5221 tcagctaggt tatttagtaa aagactagaa gattttcttt tcccaatcgt cttctgtacg
     5281 gcgaggatag aaaaactctg acttgtcggg agcttccatc atttccatca atggtagcat
     5341 atcataggcc agatttaagt ttggaatctg gtctttttgc acccaagaaa cttctccctc
     5401 ttctgaagat tgaagggtac cagagaactc agtcgcctta taacaaatga caatatagcg
     5461 cccacctgta tctagtggcc aatttttaat gccgacaagt tgaggatttt ggatagtcaa
     5521 ccctgtttct tcgtagattt cacgaatgac agactccgca aaagcctcat cattttctac
     5581 atgacctcca ggaaaggcat aaccagacca gcgattgttt tcaggggcgc gatactgcat
     5641 caccacgcgc tgagtttcga ggtcttcaat cagacagata tttgttaaaa ttgttaattg
     5701 ggaacgggac ataaatttac tcgctttcta atttattaaa ttttcagac
//
LOCUS       DAVLVP010000097         5655 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.097, whole genome shotgun sequence.
ACCESSION   DAVLVP010000097 DAVLVP010000000
VERSION     DAVLVP010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.097"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..576)
                     /locus_tag="VYN69_001766"
     CDS             complement(<1..576)
                     /locus_tag="VYN69_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1533991.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRYQAGQDKKESNRVAYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVNGKYYVYLKDAAHADNIRTKEEIKRQKQER
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNAS"
     gene            complement(734..3184)
                     /gene="phtA"
                     /locus_tag="VYN69_001767"
     CDS             complement(734..3184)
                     /gene="phtA"
                     /locus_tag="VYN69_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtA"
                     /protein_id="HEV1533992.1"
                     /translation="MKINKKYLVGSAAALILSVCSYELGLYQARTVKENNRVSYIDGK
                     QATQKTENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIFSEE
                     LLMKDPNYKLKDEDIVNEVKGGYVIKVDGKYYVYLKDAAHADNVRTKEEINRQKQEHS
                     QHREGGTPRNDGAVALARSQGRYTTDDGYIFNASDIIEDTGDAYIVPHGDHYHYIPKN
                     ELSASELAAAEAFLSGRGNLSNSRTYRRQNSDNTSRTNWVPSVSNPGTTNTNTSNNSN
                     TNSQTSQSNDIDSLLKQLYKLPLSQRHVESDGLVFDPAQITSRTARGVAVPHGDHYHF
                     IPYSQMSELEERIARIIPLRYRSNHWVPDSRPEQPSPQPTPEPSPGPQPAPNLKIDSN
                     SSLVSQLVRKVGEGYVFEEKGISRYVFAKDLPSETVKNLESKLSKQESVSHTLTAKKE
                     NVAPRDQEFYDKAYNLLTEAHKALFENKGRNSDFQALDKLLERLNDESTNKEKLVDDL
                     LAFLAPITHPERLGKPNSQIEYTEDEVRIAQLADKYTTSDGYIFDEHDIISDEGDAYV
                     TPHMGHSHWIGKDSLSDKEKVAAQAYTKEKGILPPSPDADVKANPTGDSAAAIYNRVK
                     GEKRIPLVRLPYMVEHTVEVKNGNLIIPHKDHYHNIKFAWFDDHTYKAPNGYTLEDLF
                     ATIKYYVEHPDERPHSNDGWGNASEHVLGKKDHSEDPNKNFKADEEPVEETPAEPEVP
                     QVETEKVEAQLKEAEVLLAKVTDSSLKANATETLAGLRNNLTLQIMDNNSIMAEAEKL
                     LALLKGSNPSSVSKEKIN"
     gene            complement(3437..5170)
                     /gene="ptsP"
                     /locus_tag="VYN69_001768"
     CDS             complement(3437..5170)
                     /gene="ptsP"
                     /locus_tag="VYN69_001768"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEV1533993.1"
                     /translation="MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARL
                     DAALQASQDELSVIREKAVGTLGEEAAQVFDAHLMVLADPEMISQIKETIRAKKVNAE
                     AGLKEVTDMFITIFEGMEDNPYMQERAADIRDVTKRVLANLLGKKLPNPASINEEVIV
                     IAHDLTPSDTAQLDKNFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNNITEIVKDGD
                     ILAVNGITGEVIINPTDEQAAEFKAAGEAYAKQKAEWALLKDAQTVTADGKHFELAAN
                     IGTPKDVEGVNNNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVR
                     TMDIGGDKELPYFDMPHEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRI
                     MFPMVALLKEFRAAKAVFDEEKANLLAEGVAVADNIQVGIMIEIPAAAMLADQFAKEV
                     DFFSIGTNDLIQYTMAADRMNEQVSYLYQPYNPSILRLINNVIKAAHAEGKWAGMCGE
                     MAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDTAKMEEYANRALTECSTMEEV
                     LELQKEYVNFD"
     gene            complement(5176..5439)
                     /locus_tag="VYN69_001769"
     CDS             complement(5176..5439)
                     /locus_tag="VYN69_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019775469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEV1533994.1"
                     /translation="MASKDFHVVAETGIHARPATLLVQTASKFASDITLEYKGKSVNL
                     KSIMGVMSLGVGQGADVTISAEGADADDAIAAISETMEKEGLA"
BASE COUNT         1492 a         1196 c         1046 g         1921 t
ORIGIN      
        1 agatgcattg aagatatacc catcatccgt tgtataacgt ccttgggctc tggctgcagc
       61 aacagcatta tctgctcttg agttatgatt atgactgcgt tcctgcttct gacgtttaat
      121 ctcttctttt gtccgaatat tatccgcatg agctgcatcc ttaaggtaaa catagtattt
      181 accgtttacc ttaatgacat aaccaccctt gatttcattg acaatgtctg aatccttcaa
      241 ctgataattc ggatctttca tgaggagctc ttcactgatg atggcatcat aaggaacctt
      301 gccattatag taatgataat ggtctccatg agaggtcaca taaccttgat ccgtaatctt
      361 gataacaatt tgttcggcgt tgatcccctc cctcttactg acttcatctg gtgtcaagtt
      421 ttctgccttt tgaccagcct gatcaccatc tatataagca actcgattag actctttctt
      481 atcctgacca gcttggtaac gtccaagctc ataggaacaa acacttaggg caaggactgc
      541 cactgaacct gctagatatt ttttattgat tttcattctt tcctcttttc ttaagtatat
      601 ttgattcact ataaagtaat aaaccggtta attaactagt taatagttta ctctttttaa
      661 tattgcttgt caagaatttt acatatatac ataataaaaa tgaaagcctc tttatcgaga
      721 ctttcatttt tcattagttt attttttcct tacttacaga tgaaggatta cttcctttta
      781 acaacgcaag taatttttct gcttctgcca tgatactatt gttatccata atttgaagag
      841 tcaaattatt tcgtaaacca gctagagttt ctgttgcatt ggctttcaga ctagaatccg
      901 ttactttcgc aagcaaaact tctgcttctt tgagttgggc ttctactttt tcagtctcta
      961 cttgagggac ttctggctca gcaggtgttt cctctactgg ctcttcatcc gctttgaagt
     1021 tcttatttgg atcttcactg tggtctttct tgcctaacac atgctcactg gcattgcccc
     1081 atccatcatt agaatgtgga cgttcgtcag ggtgttctac gtagtactta atcgtcgcaa
     1141 acaaatcttc caaggtatag ccatttggag ctttgtatgt gtgatcatca aaccaagcaa
     1201 atttaatgtt atggtaatga tccttatgag gaataatcaa attaccgttt ttaacctcaa
     1261 ctgtatgctc aaccatatat ggaagtcgaa cgagtggaat tcgtttttcc cctttcacac
     1321 gattgtaaat agctgctgca ctatctccag ttggatttgc tttaacatct gcgtctggag
     1381 atggaggtag gatacctttt tctttagtat aggcttgagc tgcaactttt tccttatcag
     1441 aaaggctgtc ttttccaatc cagtgactat ggcccatatg aggcgttaca tatgcatctc
     1501 cttcatcact gattatatca tgttcatcaa aaatataacc atctgacgtt gtatacttat
     1561 cagctaattg agcaatacga acttcgtctt cagtatactc aatttgagaa tttggcttgc
     1621 caagtcgctc tggatgggta attggtgcta ggaatgccaa taaatcatct accaattttt
     1681 ctttattagt cgattcatca ttcaagcgtt ctaataattt gtctaaggct tggaaatcag
     1741 aattacgacc cttattttca aacaaggctt tatgagcctc agttaacaga ttatatgctt
     1801 tatcataaaa ttcttggtca cgaggagcaa cattttcttt tttagcagtt aaagtgtgtg
     1861 aaacactctc ttgttttgat aacttgcttt caagattttt aacagtttca gatggtaaat
     1921 ctttcgcaaa gacataacga gagatgccct tttcttcgaa tacatatcct tccccaactt
     1981 ttcgtaccag ctgactaacc aaagaagaat ttgagtctat tttaagattt ggtgcaggtt
     2041 gcgggcctgg actaggttcc ggagtcggtt gtggacttgg ttgttctggc cttgaatctg
     2101 gtacccaatg gtttgaacga taacgaaggg gaataatacg agcgattcgt tcttccaatt
     2161 cagacatttg agagtaaggg atgaagtggt aatgatctcc gtgtggcact gcaacacctc
     2221 tagctgttcg acttgtgatt tgcgctgggt cgaaaacaag gccatcagat tctacatgtc
     2281 gttgactcaa aggcagtttg tagagctgtt tcaagagact atcaatgtca ttactttgac
     2341 ttgtttgact gttagtgttg ctgttgttgc ttgtgttagt atttgtagtt cctggattgc
     2401 ttacagaagg tacccagttt gttcttgaag tgttatcgct attttgtcgg cgataggttc
     2461 ttgaatttga cagatttccc cgaccagata ggaaggcttc tgcagcagcc aactcgctag
     2521 ctgataactc attcttagga atgtaatggt aatgatctcc atgaggaacg atataagcat
     2581 caccagtatc ctctatgata tcagaagcat taaagatata accatcatct gtagtatagc
     2641 gtccttgcga acgtgccaag gcaacagcac catcgtttct tggagtccca ccttcacgat
     2701 gttgactatg ttcttgtttt tgtcgattga tttcctcttt tgtacggacg ttatccgcgt
     2761 gggcagcatc cttaaggtaa acatagtatt ttccatctac cttgataaca tatccaccct
     2821 tgacctcatt aacaatatcc tcatctttta gcttatagtt cggatctttc atgagtaatt
     2881 cttcactgaa aatcgcatca taaggaacct taccattgta ataatgatag tggtcgccat
     2941 gtgaagtgac atagccttgg tctgttatct tgatgacgat ttgctcagca ttgattcctt
     3001 cacgcttgct aacctcatca ggagtcaaat tctccgtttt ttgcgtcgct tgttttccat
     3061 ctatatagga aacacgatta ttttccttaa ccgttctagc ttgatacagt cccaactcgt
     3121 aagaacaaac acttaaaatc aaagctgccg cagaaccaac aaggtatttc ttattaattt
     3181 tcatcgaaac tctctttcta tttgatattt ctagttgcaa ttcatttcta aattgcagaa
     3241 actcctctct attacttaac tggttaatag tttactccta aatctccaac ttgtcaagaa
     3301 ttttatgaac catttaagta ttttttgttc tgactactac tttttcaaga aaatctttct
     3361 ataagaaaga ttttcttgaa aattcttttt aaaatatcaa aaaaatccct gtaactgagt
     3421 tgcagggact tttcgattaa tcaaaattaa cgtattcttt ttgaagttca agaacttctt
     3481 ccattgttga gcattctgta agggcacggt ttgcgtactc ttccatctta gctgtgtcga
     3541 gtttcttcat caagctgcgt gtacgaagta cagatgttgc tgacatagag aactcatcca
     3601 agcccattcc gacaagaagt ggaacagctt gttggtcacc agccatctca ccacacatac
     3661 cagcccattt accttcagcg tgagctgctt tgatcacatt gttaatcaag cgtaggattg
     3721 atgggttgta tggttggtaa aggtatgaaa cttgttcgtt catacggtct gctgccattg
     3781 tatattggat caagtcgttt gtaccaattg agaagaagtc aacttcttta gcaaattggt
     3841 ctgcaagcat agccgctgca ggaatctcga tcatgatacc aacttggatg ttatccgcaa
     3901 ctgcaacacc ttcagcaaga aggtttgctt tttcttcatc aaagactgct ttcgctgcac
     3961 ggaattcttt caagagcgca accattggga acatgatacg caattgaccg tgaacagacg
     4021 cacgaagaag agcacggatt tgtgtgcgga acatagcatc tccagtctca gagatagaga
     4081 tacgaagagc acggaatcca aggaatgggt tcatttcgtg aggcatatcg aagtaaggaa
     4141 gttccttatc tccaccgata tccattgtac gaacgacaac aggtttaccg ttcattcctt
     4201 caagaacagc cttgtatgct tcatactgct catcttcagt tgggaagtct tgagaatcca
     4261 tgtacaagaa ctctgtacgg taaagtccaa cagcttctgc accgttgttg ttaacacctt
     4321 caacgtcttt tggagtaccg atattagcag ccaactcgaa gtgtttaccg tcagcagtca
     4381 ctgtttgagc atctttcaag agcgcccatt cagctttttg tttcgcataa gcttcaccag
     4441 ctgctttaaa ttctgccgct tgttcatcag ttgggttgat aataacctca ccagtgatcc
     4501 cgttaacagc aaggatgtca ccgtctttaa cgatttcagt gatgttgttt gtacctaata
     4561 cagctgcaat ttcaagtgta cgtgccatga tagctgagtg gcttgtacgt ccaccgatgt
     4621 tggttacaaa agcttttaca aagtttttgt ccaattgagc tgtatcagat ggtgtcaagt
     4681 catgtgcaat cacaatcact tcttcattga tagaagctgg gtttggcaat tttttaccaa
     4741 gaaggtttgc caatacacgt tttgtcacat cgcggatatc cgctgcgcgt tcttgcatgt
     4801 atgggttgtc ttccatgcct tcaaagatag tgataaacat atctgtaact tctttcagac
     4861 ctgcttctgc attcactttc ttcgcacgga tagtttcctt gatttggctg atcatttctg
     4921 ggtcagcaag aaccattaag tgagcatcaa aaacttgagc tgcttcttca ccgagcgtac
     4981 ctactgcttt ctcgcgaata acagaaagct cgtcttgtga tgcctgtaga gcggcatcaa
     5041 ggcgagcttc ttctgcgttt gtatcttcga ctgtaatagt ctcaaatgac aaatccggct
     5101 gaacgagtag atatgctttt gcaactgcaa caccgtcaga tgctgcgatt cctttaagca
     5161 tttctgtcat ttcccttatg ccaatccttc tttttccatt gtttctgaga ttgcagcgat
     5221 agcgtcatct gcatctgcac cttcagctga gatagttacg tcagcacctt ggccaacacc
     5281 aagactcata acacccataa ttgatttaag gttaactgat ttacctttgt actcaagagt
     5341 gatatctgaa gcaaatttgc tagcagtttg taccaacaat gttgctggac gtgcgtgaat
     5401 acctgtttct gccactacgt ggaaatcttt agaagccata gtttgactct ccttttgttc
     5461 tttctttttt tgagttatat gtgataaccc ttacaattta gtattatacc atcttctagg
     5521 atttttttca agcattttct ggattttcct tcaatttcct ttcagataac ttgctgaaaa
     5581 tcttctatcc tagataacaa aacacaggat atagtttctt taaaaacaat ttataggata
     5641 atcattgcta tttca
//
LOCUS       DAVLVP010000098         5655 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.098, whole genome shotgun sequence.
ACCESSION   DAVLVP010000098 DAVLVP010000000
VERSION     DAVLVP010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5655)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5655)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5655
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.098"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..210)
                     /locus_tag="VYN69_001770"
     CDS             complement(<1..210)
                     /locus_tag="VYN69_001770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1533995.1"
                     /translation="MNTIERTRRLVKGCATHCFEVVDRTDEVSSKHCFEVADRTDEVS
                     SKHVFEVVDETNEVSSKHVFEVVDET"
     gene            complement(274..1284)
                     /gene="ccpA"
                     /locus_tag="VYN69_001771"
     CDS             complement(274..1284)
                     /gene="ccpA"
                     /locus_tag="VYN69_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000990602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEV1533996.1"
                     /translation="MNADDTVTIYDVAREAGVSMATVSRVVNGNKNVKENTRKKVLEV
                     IDRLDYRPNAVARGLASKKTTTVGVVIPNITNGYFSSLAKGIDDIAEMYKYNIVLANS
                     DEDNEKEVSVVNTLFSKQVDGIIYMGYHLTDKIRSEFSRSRTPIVLAGTVDVEHQLPS
                     VNIDYKQATIDAVSYLAKENERIAFVSGPLVDDINGKVRLVGYKETLKKAGITYSEGL
                     VFESKYSYDDGYALAERLISSNATAAVVTGDELAAGVLNGLADKGVSVPEDFEIITSD
                     DSQISRFTRPNLTTIAQPLYDLGAISMRMLTKIMHKEELEEREVLLPHGLTERSSTRK
                     RK"
     gene            complement(1709..2308)
                     /locus_tag="VYN69_001772"
     CDS             complement(1709..2308)
                     /locus_tag="VYN69_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1533997.1"
                     /translation="MKVLVAEDQSMLRDAMCQLLTLQPDIESVFQAKNGQEAIQQLEK
                     ESVDIAILDVEMPVKTGLEVLEWIRAEKLETKVVVVTTFKRPGYFERAVKAGVDAYVL
                     KERSIADLMQTLHTVLEGRKEYSPELMEVVMTHPNPLTEQEIAVLKGIVQGFSNQEIA
                     DKLYLSNGTVRNYVTNILSKLDAGNRTEAANIAKESGWL"
     gene            complement(2309..3406)
                     /locus_tag="VYN69_001773"
     CDS             complement(2309..3406)
                     /locus_tag="VYN69_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001848091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1533998.1"
                     /translation="MLERLKSIHYMFWAGLIFMIFPILPVVTGWLSAWHLLIDILFVV
                     AYLGVLTTKSQRLSWLFWGIMLIYVAGNTAFVAVNYIWFFFFLSNLLSYHFSVRSLKS
                     LHVWTFLLAQVLVVGRLLIFQRIEVEFLVYLLGILTFVDLMTLGSVRIRLVEDLKEAQ
                     TKQNTQINLLLAENERNRIGQDLHDSLGHTFAMLSVKTDLALQLFQIQAYPQVEKELR
                     EIQQISKESMREVRTIVENLKSRTLTSELETVKKMLEIAGIEVEIANQLDTASLTQEL
                     ESTASMILLELVTNIIKHAKASKAYLKLERTDQELLLMVRDDGKGFETVTENELHTVR
                     ERVLPFSGEVSVISQKHPTEVQVRLPYKERK"
     gene            complement(3406..4143)
                     /locus_tag="VYN69_001774"
     CDS             complement(3406..4143)
                     /locus_tag="VYN69_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004249339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1533999.1"
                     /translation="MKRWIALNKIEFLLTKRQSVYYLLSVGMPTAFYLFFSGMYQDTP
                     GGPANFMRDYLISMTAFSMMSTAIFSFPAVLHTDKMNNWQKTLRHSPVNMVEYYLSKI
                     TSMMVDYLVSILVVFSVGHLVRGVDMSLGSWIGAAFLLIVGSIAFVALGLTLTLLPSS
                     QLMSVVGNLLYLGLAVLGGLWMPISLFPDWMQAIGKCLPTYQLMELLKTFLNEGSINL
                     SATVYLLVFSAVLFGLTIYLQGHKENA"
     gene            complement(4145..5029)
                     /locus_tag="VYN69_001775"
     CDS             complement(4145..5029)
                     /locus_tag="VYN69_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000219360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1534000.1"
                     /translation="MTVVKVEKLSKKIKDKEILRNISFEINDGECVALIGPNGAGKTT
                     LLDCLLGDKLVTSGQVYIQDLPVTSSKLDYTRSYLPQENVIVQKLKVKELIAFFQRIY
                     PNPLNNQEIDQLLQFDQQQKEQFAEKLSGGQKRLLSFILTLIGRPKLVFLDEPTAAMD
                     TSTRQRFWEIVRDLKAQGVTILYSSHYIEEVEHTADRILVLNKGELIRDTTPLAMRSE
                     EIEKHFILPLAYKEVVEKSVLVENWVQKQDALQVVTCEADAFWELLVQAGCRIQEIEV
                     NNRSLLDTIFEETQKGDD"
     gene            complement(5016..5210)
                     /locus_tag="VYN69_001776"
     CDS             complement(5016..5210)
                     /locus_tag="VYN69_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000820182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534001.1"
                     /translation="MKRGIIYFFIGLSLLVWLVEMFTDWFDQALLRQFIRGALGFGFM
                     IFVVFLMGMEWLKGESHDGG"
     gene            complement(5207..5413)
                     /locus_tag="VYN69_001777"
     CDS             complement(5207..5413)
                     /locus_tag="VYN69_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534002.1"
                     /translation="MGEEEIRNKMIIAVSLVVAGVMTYLMFSGLDEDFYHFPWKVFAG
                     FGIMSWLVREGLKLVRDVKKEFEE"
BASE COUNT         1752 a         1280 c          950 g         1673 t
ORIGIN      
        1 agtttcatct acaacctcaa aaacatgttt tgagctgact tcgttagttt catctacaac
       61 ctcaaaaaca tgttttgagc tgacttcgtc agttctatct gcaacctcaa agcagtgctt
      121 tgagctgact tcgtcagttc tatctacaac ctcaaaacag tgtgttgcgc agcctttaac
      181 cagccgccta gtccgctcta tggtattcat taagtcaaca tctcttgttt aagagcacaa
      241 aatcaggaaa tcttctcgat tccctgattt tttctattta cgttttcgtg ttgagctacg
      301 ttctgtcaaa ccatgaggta agagaacttc acgttcttcc aactcttcct tatgcataat
      361 cttggtcaac atacgcatac taatagcacc aaggtcataa agaggttggg caatcgttgt
      421 caagtttgga cgggtaaagc gtgagatttg tgaatcatca ctagtaataa tttcaaaatc
      481 ttctggcaca gaaacacctt tatcagccaa accgttcaag actcctgctg ccaactcatc
      541 acctgtcaca actgctgcag ttgcatttga tgaaatcaaa cgctctgcta aggcgtaacc
      601 atcatcatag ctatatttag attcaaatac caaaccctca ctataagtga ttcctgcttt
      661 tttcaaggtt tccttgtagc caactaaacg aaccttacca ttgatgtcat ccactagcgg
      721 accgctaacg aaagcaatac gctcattttc tttagcaagg taactcactg catcaattgt
      781 tgcttgctta tagtcaatat tgacacttgg caactggtgc tcaacatcga cagttcctgc
      841 gagaacaatc ggagtacgtg aacgcgaaaa ttctgagcga attttatctg tcaagtgata
      901 acccatatag ataatgccat ctacctgctt tgaaaagagg gtattgacaa cagaaacttc
      961 tttctcgtta tcttcatcgc tattagctag gacaatattg tacttgtaca tttctgcaat
     1021 atcatcaatc cccttagcca aactcgaaaa ataaccattg gtaatatttg gaatcacgac
     1081 accgacagtg gttgtctttt tacttgcaag accacgcgca actgcatttg gacgataatc
     1141 caaacgatca attacctcta gcactttttt acgggtattc tcttttacat ttttattgcc
     1201 attgaccaca cggctgaccg tcgccatgga aacacctgct tcacgagcga catcataaat
     1261 ggttactgta tcatctgcat tcattccttt tcctgtcctt tctatctcac acattctttt
     1321 acaagtagag gtactgattg aagctctata tctacttaca aaagtgaaga tgtgaaaatt
     1381 tcgttttcat atttctactt attccattct atcactaatt gtaaacactt tcaagtgttt
     1441 tttgaagatt gattgaaaaa atttcataga aaacctaggt ttagctcctt gctaccacct
     1501 tagactaaac aaaaaggagg aaactaagcc ctcctaaagt tatagtaaaa tgaaataaga
     1561 acaggataaa tcgatcagga cagtcaaatc gatttctaac aatgttttag aagtagaggt
     1621 gtactattct agtttcaatc tactataggt attgttccat tcactaccgt caattttagc
     1681 acatagtctt catgaaaata ttatatcatc ataaccaacc agattctttt gcgatattag
     1741 ctgcctctgt tcgattacca gcatctagtt ttgaaagaat attggtgaca tagtttcgga
     1801 cggttccgtt ggatagataa agtttgtctg caatttcttg gttagagaag ccctgaacga
     1861 ttccctttaa aactgcgatt tcttgttctg ttaatggatt gggatgcgtc atcaccactt
     1921 ccatcaattc aggcgaatac tccttgcgcc cttcgaggac agtgtgcaag gtctgcatga
     1981 gatctgcaat gcttctttct tttaatacat aagcatctac tccagccttg actgcacgtt
     2041 caaaataccc tgggcgcttg aaggtcgtca ccacaaccac ctttgtttcg agcttttctg
     2101 ctcgtatcca ctccaagact tcaagaccag tcttaacagg catctctaca tcaagaatgg
     2161 caatatctac ggactccttt tctaattgtt ggattgcttc ttgcccattc ttggcttgaa
     2221 agacagactc tatatccggt tgaagcgtga gcaattgaca catagcatct cgcaacatac
     2281 tttgatcttc tgcgactaat actttcatct actttctctc cttataaggt agtcgaactt
     2341 gcacttccgt tggatgtttc tgactgatta cacttacttc tcctgaaaat ggaaggacac
     2401 gttctcgaac tgtatggagc tcattttctg ttacagtttc aaaacctttc ccgtcatctc
     2461 tgaccatcag gagcaattcc tgatctgtgc gttctaattt taagtaggct ttagacgctt
     2521 tagcatgttt gatgatgttg gtcactaatt caagcaaaat catggaagcc gttgactcta
     2581 attcctgagt taagctagct gtatctagtt gattagctat ttccacctca attccagcaa
     2641 tttctaacat ctttttcaca gtctctagtt cggatgtcaa agttctagac ttaagatttt
     2701 ccacaatggt tcgcacttca cgcattgatt ctttgctaat ctgctgtatt tctcttaatt
     2761 ccttttccac ttgtggataa gcctgtattt gaaataactg caaggctaga tcagttttaa
     2821 cactaagcat agcaaaggta tgccccagac tatcatgcaa atcttgaccg atacgattgc
     2881 gctcattttc tgctagcaat agatttatct gggtattttg cttagtctga gcttctttca
     2941 aatcctcgac aaggcgaatt cgaaccgagc ccaaagtcat caaatcaaca aaagtaagaa
     3001 ttccaagcag atagactaga aactcaactt cgattctctg aaaaatcaaa agtcgcccca
     3061 caacaaggac ttgagcaaga agaaaagtcc agacatgtaa agactttaaa ctacgtacgc
     3121 tgaaatgata acttaagaga ttggaaagga aaaagaaaaa ccagatataa ttcacagcaa
     3181 caaaggcggt attcccagct acatagatca gcatgatccc ccagaaaagc caagataggc
     3241 gctggctctt agttgttaaa acacccaaat atgccactac aaatagaata tcaatcaata
     3301 aatgccaggc agaaagccac ccagtcacta caggtaggat ggggaaaatc ataaaaatta
     3361 aaccggccca aaacatatag tgtatgcttt tcagtctttc aagcattaag cattctcctt
     3421 atgaccttga aggtaaatgg tcaaaccaaa caaaactgct gaaaaaacaa gtagataaac
     3481 tgtggctgat agattgatgc taccctcgtt taagaaggtc ttgagcaact ccatcaactg
     3541 ataagttggt aggcacttcc cgattgcttg catccagtct ggaaataaag agatgggcat
     3601 ccagagtcca cctaaaacag ccaagcctag atagagaaga ttgcccacga cagacatcag
     3661 ctgactagaa ggcaagagtg tcaaggtcaa gccaagcgct acaaaagcta tacttcctac
     3721 tatcagcaag aacgcagccc caatccagct tcctaaagac atatccacac ctctgaccaa
     3781 gtgcccaact gagaaaacaa ccaggattga gaccaaataa tcaaccatca tacttgttat
     3841 ctttgataga taatattcta ccatatttac agggctatga cgcaatgttt tctgccagtt
     3901 gttcatctta tcggtatgta agacagctgg gaatgaaaag atagcggtcg acatcatgga
     3961 aaaggcagtc atggagatga ggtagtcgcg cataaaatta gccggtccac ctggtgtgtc
     4021 ctggtacatg cctgaaaaga atagatagaa ggctgtcggc atccctacag ataataaata
     4081 atagactgat tgccgtttgg tcaatagaaa ttctatcttg tttagtgcaa tccatcgttt
     4141 catcttagtc atctcccttc tgtgtttctt caaagattgt atccaacaaa ctacgattat
     4201 tgacttcgat ttcttgtatc ctacatcctg cttgaactaa cagttcccag aaagcatccg
     4261 cttcgcatgt gactacttgt aaggcatctt gtttttgtac ccagttttca accaagactg
     4321 acttctcaac gacttccttg taagctagcg gaaggataaa atgcttttca atctcctcac
     4381 tacgcatagc tagaggcgtc gtgtcacgaa tcaactctcc cttatttaaa accaaaatcc
     4441 ggtcagctgt atgctctacc tcttcaatat aatgagacga atagagaatg gtgactcctt
     4501 gcgcttttag gtcccgaaca atttcccaaa aacgttgacg agttgaagta tccatggcag
     4561 cagttggctc atctaaaaag acaagctttg gtcgcccaat taaggtcaag ataaaagata
     4621 aaagacgctt ttgcccacct gacaattttt ctgcgaattg ctctttctgt tgctggtcaa
     4681 actgtaatag ttgatcgatt tcctggttgt tcaagggatt tggatagata cgttgaaaga
     4741 aagcaatcaa ctctttaacc tttagttttt gaacaattac attttcttga gggagatagg
     4801 atctcgtata gtctaactta gagctcgtta ccggcaaatc ttggatatat acttgcccac
     4861 tggtgaccaa tttatctcca agcaaacaat ctaagagtgt tgtctttccg gcaccatttg
     4921 gaccaattaa ggcaacacat tcaccatcgt tgatttcaaa ggagatattc cgcaaaattt
     4981 ccttgtcttt tattttctta ctcaatttct caactttaac caccgtcatg agactctcct
     5041 ttcaaccact ccattcccat aaggaaaacg acgaaaatca taaatccaaa ccccaaagca
     5101 ccacgaatga attggcgaag caaggcttga tcaaaccaat cagtaaacat ttccactaac
     5161 cataccaaga gtgacaggcc gataaagaaa tagatgatcc ctctcttcat tcctcaaact
     5221 cctttttcac atctctgact aatttcaaac cttctctaac aagccaagac atgattccaa
     5281 agccagcaaa gaccttccaa ggaaaatggt agaaatcctc atccaatccc gaaaacatga
     5341 gataggtcat aactcctgct actactaaac ttactgcgat aatcatttta tttcttatct
     5401 cttcttcccc catttcatac tacaattata gtcttttaaa atcagaggag acagaagctt
     5461 ctgtcactag aaaatatgac aaatgtcata aaaaattctg ttcaaaacaa gcaagataca
     5521 ctatacaata aaacacaatc agaaaaatct aaggcaactt cctcaaaaga gatatcaaac
     5581 ccaattcaca ccataatgta aactaatact tatttaaaat caaaaagagt agaaattttt
     5641 atcagacaaa cacat
//
LOCUS       DAVLVP010000099         5625 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.099, whole genome shotgun sequence.
ACCESSION   DAVLVP010000099 DAVLVP010000000
VERSION     DAVLVP010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5625
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.099"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            431..1108
                     /locus_tag="VYN69_001778"
     CDS             431..1108
                     /locus_tag="VYN69_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEV1534003.1"
                     /translation="MISKRLELVASFVSQGAILLDVGSDHAYLPIELVERGQIKSAIA
                     GEVVEGPYQSAVKNVEAHGLKEKIQVRLANGLAAFEETDQVSVITIAGMGGRLIARIL
                     EEGLGKLANVERLILQPNNREDDLRIWLQDHGFQIVAESILEEAGKFYEILVVEAGQM
                     KLSASDVRFGPFLCKEVSPVFVQKWQKEAVKLEFALGQIPDKNLEERQVLVDKIQAIK
                     EVLHVSK"
     gene            1095..1892
                     /locus_tag="VYN69_001779"
     CDS             1095..1892
                     /locus_tag="VYN69_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000881127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEV1534004.1"
                     /translation="MLASEVIQAYEAFCPQEFSMEGDSRGLQIGTLDKGIQRVMVALD
                     IREETVAEAIEKGVDLIIVKHAPIFRPIKDLLASRPQNQIYIDLIKHDIAVYVSHTNI
                     DIVENGLNDWFCQMLEIEETTYLQETGPERGIGRIGNIQPQTFGELAQHVKQVFDLDS
                     LRMVHYQENDLQKPISRVAICGGSGQSFYKDALAKGADVYITGDIYYHTAQDMLSDGL
                     LALDPGHYIEVLFVEKIAALLTQWKEKKGWELEILPSQASTNPFRHI"
     gene            1909..3012
                     /locus_tag="VYN69_001780"
     CDS             1909..3012
                     /locus_tag="VYN69_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEV1534005.1"
                     /translation="MKKVAIIGAGIVGATAAYYLSRESDLEVTVFDHEQGQATKAAAG
                     IISPWFSKRRNKAWYKMARLGADFYVDLLADLEKSGQEIDFYQRSGVFLLKKDESNLE
                     ELYQLALQRREESPLIGQLAILNQASANELFPGLQGFDRLLYASGGARVDGQLLVTRL
                     LEASHVKLVKEKVTLTPLASGYQIGEEEFEQVILATGAWLGDMLEPLGYEVDVRPQKG
                     QLRDYQLVQDMEAYPVVMPEGEWDLIPFAGGKLSLGATHENDMGFDLTVDETLLQQME
                     EATLTHYLILAEATSKSERVGIRAYTSDFSPFFGQVPDLTGVYAASGLGSSGLTTGPI
                     IGYHLAQLIQDKELTLDPENYPIENYVKRVKSE"
     gene            3082..3255
                     /locus_tag="VYN69_001781"
     CDS             3082..3255
                     /locus_tag="VYN69_001781"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534006.1"
                     /translation="MDRTDEVSSKHCFEVVDRTDEVSSKHCFEVVDRTDEVSNHTHGK
                     ATLTWFELDFRRV"
     gene            3358..4377
                     /gene="galE"
                     /locus_tag="VYN69_001782"
     CDS             3358..4377
                     /gene="galE"
                     /locus_tag="VYN69_001782"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006153709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1534007.1"
                     /translation="MQEKILVTGGAGFIGTHTVIELIQAGHQVVVVDNLVNSNRKSLE
                     VVERITGVEIPFYEADIRDTATLRDIFKQEEPTGVIHFAGLKAVGESTRIPLAYYDNN
                     IAGTVSLLKAMEENNCKNIIFSSSATVYGDPHTVPILEDFPLSVTNPYGRTKLMLEEI
                     LTDIYKADSEWNVVLLRYFNPIGAHESGDLGENPNGIPNNLLPYVTQVAVGKLEQVQV
                     FGDDYDTEDGTGVRDYIHVVDLAKGHVAALKKIQKGSGLNVYNLGTGKGYSVLEIIQN
                     MEKAVGRPIPYRIVERRPGDIAACYSDPAKAKAELGWEAELDITQMCEDAWRWQSKHP
                     NGFED"
     gene            4379..5314
                     /locus_tag="VYN69_001783"
     CDS             4379..5314
                     /locus_tag="VYN69_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEV1534008.1"
                     /translation="MMISIIVPCLNEEEVLPLFYQALEALLPDLETEIEYVFVDDGSS
                     DGTLELLKAYREQNPAVHYISFSRNFGKEAALYAGLQYATGDLVVVMDADLQDPPSML
                     FEMKNVLDKNVDLDCVGTRRTSREGEPFFRSFCAVLFYRLMQKISPVALPSGVRDFRM
                     MRRSVVDAILSLTESNRFSKGLFAWVGFKTYYLDYPNVERQAGKTNWSFRQLFFYSIE
                     GIINFSDFPLIIAFVAGLLSCFLSLLMTFFVVVRTLILGNPTSGWTSLMAVILFLGGI
                     QLLTIGILGKYISKIYLETKKRPLYLIKEKSYHRS"
     gene            5489..>5625
                     /locus_tag="VYN69_001784"
     CDS             5489..>5625
                     /locus_tag="VYN69_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534009.1"
                     /translation="MLSKEEYIEEIGLIEKQNYVEVELYPLVADIINPTLKNSLSKRY
                     V"
BASE COUNT         1642 a         1037 c         1316 g         1630 t
ORIGIN      
        1 aatacttagg gagacctgtg acaatgatat cttcctctgg aagtagttct aattcaaaca
       61 atgtttgttc catgactgta tcctcctgtt atgataaatt ccagaaagca tagagcaaat
      121 tcgtggttcg tctgactaaa tttcttatct gaacaccacc ttcaaataca aattttagtc
      181 aaacttttat tttttctaac tttctaatta tattctacca tgtgttttta tatgtcaaga
      241 attttctgaa taaactgttt attttttatt ttttgacatt tttgcaaatt gtttaaaatc
      301 tttcaattgg attcacttat tactttagga cattgaaact tctttcgcca gaggtgctta
      361 tattactggg aagttgaaga ttgaaatgtg gtataataag aggtaataga gttttggaaa
      421 gtgagagaag atgatttcaa agagattaga attggtagct tcctttgtgt cacagggggc
      481 tattttacta gatgtgggaa gtgaccatgc ttatctgcct atcgagttgg ttgagagagg
      541 ccaaatcaaa agcgctattg caggtgaggt ggtggaaggt ccctatcagt ctgcggttaa
      601 aaatgttgag gctcacggcc taaaggagaa aatccaagtc cgtttagcca atggcttggc
      661 agcttttgaa gagactgacc aagtgtctgt cattaccatt gctggcatgg gtggtcgttt
      721 gattgctagg attttagaag aaggtttggg gaagttagct aatgtagagc gtttgatcct
      781 ccagcccaat aatcgtgaag acgacttgcg tatctggcta caggatcatg gattccagat
      841 tgtagcagaa agcatcttag aagaagctgg aaagttttat gagattttgg tggtggaagc
      901 aggacaaatg aagctatcag ccagtgatgt tcgctttgga ccgttcttgt gcaaagaggt
      961 cagtccagtc tttgtccaaa aatggcaaaa agaagctgtt aagctagagt ttgccctcgg
     1021 acaaatccca gacaaaaatc tggaagaacg tcaagttcta gtagataaga ttcaagccat
     1081 caaggaggtg ctccatgtta gcaagtgaag tgatccaagc ttatgaagcc ttttgccctc
     1141 aggaattttc tatggaggga gacagccgtg gtctgcaaat tggcacttta gacaagggta
     1201 tccaaagggt catggttgcc ctcgatattc gtgaagagac agtggcagag gccattgaaa
     1261 agggtgtgga cttgattatc gtcaagcacg cgcctatctt tcgtcctatc aaggacttgc
     1321 ttgccagccg tccgcaaaat cagatttaca tcgacctgat taagcatgac atcgcagttt
     1381 atgtcagcca taccaatatt gatatcgttg aaaatgggct caatgactgg ttttgtcaga
     1441 tgctagaaat cgaggagacg acttatctgc aggaaacagg tccagaacgt ggaattggac
     1501 gtattgggaa tattcagcct cagacctttg gagaattggc ccaacatgtc aagcaagtct
     1561 ttgacctaga tagccttcga atggtgcatt atcaagagaa tgatttgcag aagcctattt
     1621 caagagtagc aatttgtggt ggaagcgggc agtctttcta taaggatgct ttggcaaagg
     1681 gggcagatgt ctatatcact ggtgatatct actaccatac tgctcaggat atgctgtctg
     1741 atggcttgtt agcattggat ccaggtcact atatcgaagt gctttttgtg gaaaaaatcg
     1801 cagcactcct tactcaatgg aaagagaaaa aaggctggga actagagatt ttacctagtc
     1861 aagcatcgac caatcctttc cgtcatatct agttagaaag tgaagacaat gaaaaaagtt
     1921 gccattattg gagcaggaat tgtgggagca acagctgcct actacctctc gagagaaagt
     1981 gacctagagg tgaccgtttt tgaccatgag caaggtcaag ccaccaaggc cgcagcagga
     2041 attatcagtc cttggttttc caaacgccgt aataaagcct ggtacaagat ggcgcgcttg
     2101 ggggctgatt tttatgtgga tttattagct gatttagaga aatcaggaca agaaatcgac
     2161 ttttaccagc gttcgggagt ctttctcttg aaaaaggatg aatccaattt ggaagaactt
     2221 tatcaactgg ccctccagcg cagagaagaa tctcccttga tagggcaatt agccattctg
     2281 aaccaagcct cagctaatga attattccct ggtttgcagg gatttgaccg cctgctctat
     2341 gcttctggtg gagcgagagt agatggccaa ctcttggtaa ctcgtctgct agaagccagt
     2401 catgtcaagc tggtcaaaga aaaagtgact ctgacaccgt tagcatcagg ctaccagatt
     2461 ggtgaagagg agtttgagca ggttattttg gcgacgggag cttggttggg ggacatgtta
     2521 gaacccttgg gttatgaagt agatgttcgt cctcaaaaag gacaactacg agattatcag
     2581 ctggtccaag atatggaagc ttaccctgtt gtcatgccag aaggggagtg ggatttgatt
     2641 ccttttgcag gtgggaaatt gtccttaggc gctacccacg aaaatgacat gggatttgat
     2701 ttgacggtag atgaaacctt gctccaacaa atggaggagg ccaccttgac tcactatctg
     2761 attttggctg aagctacttc aaaatctgag cgtgtgggaa tccgtgccta caccagtgat
     2821 ttctctcctt tctttgggca ggtgcctgac ttaactggtg tctatgcagc cagtggatta
     2881 ggttcatcag gcctcacaac tggtcctatt attggttacc atttagctca actgatccaa
     2941 gacaaggagt tgaccttgga ccctgaaaac tacccaattg aaaactatgt caaacgagta
     3001 aaaagcgaat aatactcaat gaaaatcaaa gaacaaacta ggtaactagc cgcgggttac
     3061 tcaaagcact gctttgaggt tgtggataga actgacgaag tcagctcaaa acactgtttt
     3121 gaggttgtag atagaactga cgaagtcagc tcaaaacact gttttgaggt tgtagataga
     3181 actgacgaag tcagtaacca tacccatggc aaggcgacgt tgacgtggtt tgaattggat
     3241 tttcgaagag tataagcatt ttactgaaat tttatcaggt agtttaggat ggcaaatgac
     3301 attccctgtc aaaaatggta aaataagaaa aaataatctg agaatcgagg aaaaaagatg
     3361 caagaaaaga ttttggtaac tggtggtgcc ggttttattg gaacccacac tgttattgaa
     3421 ttgatccaag caggccatca agttgttgtg gtggataatc ttgtcaatag caatcgtaag
     3481 agtttagaag ttgttgaaag aattacagga gttgagattc ctttctatga ggcagatatt
     3541 cgtgacacag ctaccctcag agatattttc aagcaggaag aaccgactgg tgtcattcac
     3601 tttgctggtt tgaaggctgt tggcgaatca acacgtatcc ctcttgccta ctatgacaac
     3661 aatatcgctg gaactgtcag ccttttgaaa gccatggaag aaaacaactg taaaaacatc
     3721 atcttcagtt cttctgcgac agtttacggg gatccgcaca cagtgcccat cttggaagat
     3781 ttcccacttt cagtgaccaa cccatacggt cgtaccaagc tcatgctaga ggaaattttg
     3841 accgatattt acaaagcaga ctcagaatgg aatgttgtct tgcttcgtta ctttaaccca
     3901 atcggagccc atgagagtgg tgatttggga gaaaatccaa acggtattcc aaacaatctc
     3961 ttgccatatg tgactcaagt agccgttgga aaattagagc aagtgcaagt gtttggagac
     4021 gattacgata cggaagatgg aacaggtgtt cgtgactata tccacgttgt cgatttggct
     4081 aagggtcacg ttgcagcttt gaaaaaaatc caaaaaggtt caggactaaa cgtttataac
     4141 cttggaactg gtaaaggtta ctcagttctt gaaattatcc aaaacatgga aaaagcggtg
     4201 ggacgtccta ttccttaccg catcgtagaa cgtcgcccag gtgatatcgc tgcctgctac
     4261 tcagacccag caaaagctaa agcagaactc ggttgggaag cagaactcga catcacccaa
     4321 atgtgtgaag acgcatggcg ttggcaaagc aagcatccaa atggatttga agactaagat
     4381 gatgatttca atcatcgtcc cttgtttaaa cgaagaggaa gtacttcctc ttttttatca
     4441 ggctctggaa gctttacttc cagatttgga aacagaaatc gagtatgtct ttgtcgatga
     4501 tggatcaagt gatgggacct tggaactctt aaaggcctat cgggagcaaa atccggcagt
     4561 ccattatatt tctttctctc gaaattttgg caaagaagca gccctttatg caggcttgca
     4621 atatgcgaca ggagatttgg tggtggtgat ggatgcagac ctccaagatc ctcctagtat
     4681 gttgtttgag atgaaaaatg tactagacaa aaatgtagac ttggactgcg ttgggacacg
     4741 gagaactagt cgggagggag aacccttctt tcgcagtttc tgtgctgttc tcttttatcg
     4801 cctcatgcaa aaaatcagcc cagtagctct gccgtcgggt gtccgtgatt ttcgtatgat
     4861 gagaaggtct gtagtcgatg ccattttaag cttgaccgag tccaatcgtt tttcaaaggg
     4921 gctcttcgcc tgggtgggtt tcaaaaccta ctatttggac tatccaaatg tcgaaaggca
     4981 ggctggcaag acaaattgga gttttaggca actctttttc tactccattg aagggattat
     5041 taatttttca gattttccct tgattatagc ctttgtagct ggtctcctat cttgttttct
     5101 ttctctgctg atgacctttt ttgttgtggt tcggaccctc attttgggca atccgacatc
     5161 tggttggacc tctctgatgg ctgttattct ctttcttgga ggtattcaac tcttgaccat
     5221 tgggattctc ggcaagtata tcagtaagat ttatttagaa accaaaaaaa gaccgcttta
     5281 tcttatcaaa gaaaaaagtt atcatagatc atgaaaaagt aggagttatc tagcgcagtc
     5341 acttttttgt aattataatt cttttggtct ggaacttgag tgatttttaa aaaaattagg
     5401 gatttgacat gatataaatg aactatgtgg aataaattta ctgcaaagac gataacttaa
     5461 taaataaaaa attcaggagg gttattttat gctaagtaaa gaggagtata ttgaagaaat
     5521 cgggcttata gagaaacaaa attatgtaga ggttgaactg tatcctttag ttgctgatat
     5581 aattaatccg actttaaaaa atagtttatc aaaaagatat gtctt
//
LOCUS       DAVLVP010000100         5609 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.100, whole genome shotgun sequence.
ACCESSION   DAVLVP010000100 DAVLVP010000000
VERSION     DAVLVP010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5609
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.100"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            347..1393
                     /gene="pheS"
                     /locus_tag="VYN69_001785"
     CDS             347..1393
                     /gene="pheS"
                     /locus_tag="VYN69_001785"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013903845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEV1534010.1"
                     /translation="MSTIEEQLKALREETLTSLKQITAGNEKEMQDLRVSVLGKKGSL
                     TEILKGMKDVSAEMRPIIGKHVNEARDVLTAAFEETAKLLEEKKVAAQLASESIDVTL
                     PGRPVATGHRHVLTQTSEEIEDIFIGMGYQVVDGFEVEQDYYNFERMNLPKDHPARDM
                     QDTFYITEEILLRTHTSPVQARAMDAHDFSKGPLKMISPGRVFRRDTDDATHSHQFHQ
                     IEGLVVGKNISMADLQGTLQLIVQKMFGEERQIRLRPSYFPFTEPSVEVDVSCFKCGG
                     EGCNVCKKTGWIEIMGAGMVHPRVLEMSGIDATVYSGFAFGLGQERVAMLRYGINDIR
                     GFYQGDVRFSEQFK"
     gene            1393..1902
                     /locus_tag="VYN69_001786"
     CDS             1393..1902
                     /locus_tag="VYN69_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1534011.1"
                     /translation="MIRKVEMADVEVLAKIAKQTFRETFAYDNTEEQLQEYFEEAYSL
                     KTLSTELGNPDSETYFIMHEEEIAGFLKVNWGSAQTERELEDAFEIQRLYVLQKFQGF
                     GLGKQLFEFALELATKNSFSWAWLGVWEHNTKAQAFYNRYGFEKFSQHHFMVGQKVDT
                     DWLLRKKLR"
     gene            1979..4384
                     /locus_tag="VYN69_001787"
     CDS             1979..4384
                     /locus_tag="VYN69_001787"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEV1534012.1"
                     /translation="MLVSYKWLKELVDIDVPSQELAEKMSTTGIEVESVESPAADLSK
                     IVVGEVLSCEDVPETHLHVCQVNVGEEEARQIVCGAPNVRAGIKVMVALPGARIADNY
                     KIKKGKIRGLESLGMICSLGELGISDSVVPKEFADGIQILPEDAVPGEEVFSYLDLDD
                     EIIELSITPNRADALSMCGVAHEVAAIYDKAVNFKEFTLTETDQVAADALSVGIETDK
                     APYYAARILDNVTIAPSPQWLQNLLMNEGIRPINNVVDVTNYILLYFGQPMHAFDLDT
                     FEGTDIRVREARAGEKLVTLDGEERDLDVNDLVITVADKPVALAGVMGGQATEISEKS
                     SRVVLEAAVFNGKSIRKTSGRLNLRSESSSRFEKGINVATVNEALDAAASMIAELAGA
                     TVRKGIVSAGELDTSDVEVSSTLADVNRVLGTELSYADVEDVFRRLGFGLSGNADSFT
                     VSVPRRRWDITIEADLFEEIARIYGYDRLPTSLPKDDGTAGELTATQKLRRQVRTIAE
                     GAGLTEIITYTLTTPEKAVEFTAQPSNLTELMWPMTVDRSVLRQNMISGILDTVAYNV
                     ARKNKNLALYEIGKVFEQTGNPKEELPNEINSFAFALTGLVAEKDFQTAAVPVDFFYA
                     KGILEALFARLGLQVTYTATSEIASLHPGRTAVISLGDQVLGFLGQVHPVTAKAYDIP
                     ETYVAELNLSAIEAALQPAAPFVEITKFPAVSRDVALLLKAEVTHQEVVDAIQAAGVK
                     RLTDIKLFDVFSGEKLGLGMKSMAYSLTFQNPEDSLTDEEVARYMEKIQASLEEKVNA
                     EVR"
     gene            complement(4452..5453)
                     /locus_tag="VYN69_001788"
     CDS             complement(4452..5453)
                     /locus_tag="VYN69_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000597989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEV1534013.1"
                     /translation="MIKTFLSALSVILFSIPIITYSFFPSSNLNIWLSTQPILAQIYA
                     FPLATATMAAILSFLFFFLSFYKKNKQIRFYSGILLLLSLILLLFGTDKTLSSASNKT
                     KTLKLVTWNVANQIEAQHIERIFSHFDADMAIFPELATNIRGEQENQRIKLLFHQVGL
                     SMANYDIFTSPPTNSGIAPVTVIVKKSYGFYTEAKTFHTTRFGTIVLHSRKQNIPDII
                     ALHTAPPLPGLMEIWKQDLNIIHNQLASKYPKAIIAGDFNATMRHGALAKISSHRDAL
                     NVLPPFERGTWNSQSPKLFNATIDHILLPKNHYYVKDLDIVSFQNSDHRCIFTEITF"
BASE COUNT         1670 a         1058 c         1291 g         1590 t
ORIGIN      
        1 cccaatcgat ttgttctcat cttatttcaa tccgctataa agttagataa gaataagcta
       61 acaagcagaa gtaagatagg tcttgcaaga atggtaaaaa tctgataaac taagtagtaa
      121 ttgaatagga atctgaaaga ctagtagccc aagggaagta tatcagggag gagagccgtg
      181 actgcaagct ctctatatga aagctgggtg aattcacttg cgcatggatt tagaaatgaa
      241 ggtctgactt gtcagatgaa aacggatggt accgcgtgtc aacgctccga gtggagtttt
      301 tggcatgtgg ttttcttttt atctacgaga gactgatgga ggaaatatgt caactattga
      361 agaacaatta aaagcgcttc gcgaagaaac gctgactagc ttgaagcaga ttactgctgg
      421 aaatgaaaaa gagatgcaag atttgcgtgt ctctgtcctt ggtaaaaagg gttcgctcac
      481 tgaaatcctc aaagggatga aagatgtttc tgctgagatg cgtccaatca tcgggaaaca
      541 cgtcaatgaa gctcgtgatg tcttgactgc tgcttttgaa gaaacagcta agctcttgga
      601 agaaaagaaa gtcgcggctc aactggctag cgagagtatc gatgtgacgc ttccaggtcg
      661 tccagttgcg actggtcacc gtcacgtttt gacacaaacc agtgaagaaa tcgaagatat
      721 cttcatcggt atgggttatc aagtcgtgga tggttttgaa gtggagcaag actactataa
      781 ctttgaacgt atgaaccttc caaaagacca cccagctcgt gatatgcagg atactttcta
      841 tatcactgaa gaaatcttgc tccgtaccca cacgtctcca gttcaggcgc gtgctatgga
      901 tgcccatgat ttttctaaag gtcctttgaa gatgatctcg ccagggcgtg tcttccgtcg
      961 cgatacggac gatgcgaccc acagtcacca attccaccaa atcgaaggct tggtagttgg
     1021 gaaaaatatc tctatggctg atcttcaagg aacgcttcag ttgattgtcc aaaaaatgtt
     1081 tggtgaagag cgtcaaattc gtttgcgtcc atcttacttc ccattcacag agccatctgt
     1141 tgaggtggat gtttcttgct tcaagtgtgg tggagaaggc tgtaatgtat gtaagaaaac
     1201 aggttggatc gaaattatgg gggccggtat ggttcaccca cgtgtccttg aaatgagtgg
     1261 tatcgatgcg actgtatact ctggctttgc ctttggtctt ggacaagagc gtgtagctat
     1321 gctccgttat ggaatcaacg atatccgtgg attctaccaa ggagatgtcc gcttctcaga
     1381 acagtttaaa taatgattag aaaagtagaa atggcagatg ttgaggtgtt ggctaaaatt
     1441 gccaaacaaa cctttcgtga aacctttgcg tatgataata cggaagagca gttacaggaa
     1501 tactttgaag aagcttatag tctaaaaact ttgtcaactg agttgggaaa tcctgactct
     1561 gaaacctatt tcattatgca tgaggaggag atagctggtt ttctcaaagt caactgggga
     1621 agtgctcaaa ctgagagaga attagaggac gcttttgaaa ttcaacgcct ctatgtgcta
     1681 caaaaattcc aaggatttgg actaggtaag caactgtttg aattcgcact tgaacttgct
     1741 acaaaaaata gtttttcttg ggcttggtta ggtgtttggg agcataatac aaaagctcaa
     1801 gcgttttata atcgatatgg ttttgaaaaa tttagccaac atcattttat ggttggtcaa
     1861 aaagtagata cggattggtt actgagaaag aaattaaggt aagaagagga ttcttctatt
     1921 gaaatgatag gaaaggaaaa gaactagagt gacaactgtc attctggaga actaaattat
     1981 gcttgtatct tataaatggt taaaagaatt ggtggacatt gatgtgccat cacaggagtt
     2041 ggctgaaaaa atgtcaacca cagggatcga ggtagagagt gtcgaatcac cagctgctga
     2101 tctctcaaaa attgttgtcg gtgaggtctt gtcttgcgaa gatgtgccag agactcacct
     2161 ccatgtttgt caggttaacg ttggcgaaga agaagcccgt cagatcgttt gtggtgcccc
     2221 aaatgtgcgt gctgggatca aggtcatggt ggctcttcca ggagctcgta tcgctgataa
     2281 ctacaaaatc aaaaaaggaa aaatccgtgg tttggagtca cttggaatga tctgttcact
     2341 tggtgaattg gggatttctg actcagttgt gcctaaggaa ttcgcagatg gcatccaaat
     2401 cttgcctgaa gatgccgtgc caggtgagga agtcttttct tacctagact tggatgatga
     2461 aatcatcgaa ctttccatca caccaaaccg tgcagatgcc ctttctatgt gtggagtggc
     2521 tcacgaagtg gcagccatct atgacaaggc agtcaacttt aaagaattta ctttaacaga
     2581 aacagaccaa gttgcagccg atgccctttc tgtgggcatt gagacagaca aggcacctta
     2641 ctatgcggcc cgtatcttgg acaatgtgac catcgcacca agtccacaat ggttgcaaaa
     2701 ccttctcatg aacgaaggca tccgtcccat caataacgtt gtagacgtga caaactacat
     2761 cctgctctac tttggtcaac ctatgcatgc ttttgacttg gacacatttg aagggactga
     2821 catccgtgtg cgtgaagcgc gtgctggtga aaaattggtg accttggacg gtgaagaacg
     2881 tgacttggac gtgaatgacc tagtcatcac tgtcgcagac aagccagtag cccttgcagg
     2941 tgtcatgggt ggtcaagcaa cagaaatctc tgaaaaatct agtcgtgttg tccttgaagc
     3001 tgctgttttc aatggcaaat ctatccgtaa gacaagtggt cgcctgaacc ttcgttcaga
     3061 atcgtcttct cgctttgaaa aaggcatcaa tgtggcaaca gtcaatgaag cccttgatgc
     3121 agcagctagc atgattgcag agcttgcagg tgcgacggtg cgtaagggta tcgtttcagc
     3181 gggtgagctt gatacttcag atgtagaagt ttcttcaact cttgctgatg ttaaccgtgt
     3241 cctcggaact gagctgtctt atgctgatgt cgaagacgtc ttccgtcgtc ttggctttgg
     3301 tctttctgga aatgcagaca gctttacagt cagcgtacca cgtcgtcgtt gggatatcac
     3361 aatcgaagct gacctctttg aagaaattgc tcgtatctat ggttatgacc gcttgccaac
     3421 tagtctacca aaagacgatg gtacagcagg tgaattgaca gccacacaaa aactccgccg
     3481 tcaagttcgt actattgctg aaggagcagg tttgacagaa atcatcacct atactctaac
     3541 aactcctgaa aaagcagttg agtttacggc tcaaccaagt aaccttacgg aacttatgtg
     3601 gccaatgact gtggatcgtt cagtcctccg tcaaaatatg atttcaggta tccttgatac
     3661 cgttgcctac aacgtggctc gtaagaataa aaacttggcc ctttacgaga ttggaaaagt
     3721 ctttgaacaa acaggtaatc caaaagaaga acttccaaat gaaatcaaca gttttgcctt
     3781 tgccttgaca ggcttggttg ctgaaaaaga tttccaaaca gcagcagttc cagttgattt
     3841 tttctatgct aagggaatcc ttgaagccct atttgctcgt ttgggactcc aagtaaccta
     3901 tacagcaaca tctgaaatcg ctagccttca tccaggtcgt acagcagtca tttcactcgg
     3961 tgaccaagtt cttggtttcc ttggccaagt gcatccagtc actgctaagg cttatgatat
     4021 tccagaaacg tatgtagctg agcttaacct ttcagctatc gaagctgcgc ttcagccagc
     4081 ggctccattt gtagaaatca ccaaattccc ggcagtcagc cgtgacgttg cccttctcct
     4141 caaggcagaa gtgactcatc aagaagttgt agatgctatc caagctgccg gcgtgaaacg
     4201 tttgacagat atcaaacttt ttgacgtctt ctcaggtgag aaattgggac ttggtatgaa
     4261 gtcaatggct tatagcttga ccttccaaaa tccagaagat agcttaacgg acgaagaagt
     4321 cgcacgctat atggaaaaaa tccaagcatc gctcgaagaa aaagtcaatg cagaagtgcg
     4381 ttaaacgata gtatgataca aattccccct ctagtttatg aacactagag gggtgatttt
     4441 atataaaata attaaaatgt gatttctgta aaaatacatc tatgatcaga gttttgaaaa
     4501 cttacaatgt ctaaatcttt aacatagtag tggtttttag gcaataaaat atgatctatt
     4561 gttgcattaa aaagttttgg actttggcta ttccaagttc ctctttcaaa aggtggcagt
     4621 acatttaatg cgtccctatg agagcttatt tttgcaagtg ctccatgacg catagttgca
     4681 ttaaaatcac ctgcaataat agcctttgga tattttgaag ccaattgatt atgaatgatg
     4741 tttaagtctt gcttccagat ttccattaaa cctggcagag gaggagcagt atgcaaggca
     4801 atgatatctg gtatattttg ttttctcgaa tgtaatacaa ttgtcccgaa ccgtgttgta
     4861 tgaaaagttt tagcttctgt atagaaacca taacttttct tgacaatcac agtcacagga
     4921 gctattccac tattggtagg tggagaagtg aaaatatcat agttggccat agaaagtcca
     4981 acttgatgaa acaatagttt gattctctgg ttttcttgct cacctctgat attggtagct
     5041 agttcaggga atatagccat atcggcgtca aaatggctaa aaattcgctc aatatgttgt
     5101 gcttctattt gattagcgac gttccaagtt actaatttta aggttttagt cttatttgat
     5161 gcagaagaaa gggttttatc tgttccgaat aatagtaata tgagcgatag taagagcaaa
     5221 atgccagagt aaaaccgtat ttgtttattt ttcttgtaaa aagataggaa aaaaaataag
     5281 aaacttaaaa tagcagccat agttgcagta gctaagggga aggcataaat ctgtgccaag
     5341 ataggttggg tagatagcca aatgttaaga ttagaagatg ggaaaaaaga ataagttatg
     5401 atagggatag aaaagagaat gaccgaaagg gcagagagaa atgttttaat cattgtgtga
     5461 gtacctcaat ttgcaatggt tgctcggagc taatgtgact ttgtgacttt acttgattga
     5521 tattcttttt cttgtcttcc tcatagatta agtacaaccc aagtaaaaaa agtccaattt
     5581 aagattgaaa tgattaaaag gcacagaga
//
LOCUS       DAVLVP010000101         5452 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.101, whole genome shotgun sequence.
ACCESSION   DAVLVP010000101 DAVLVP010000000
VERSION     DAVLVP010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5452
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.101"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(358..909)
                     /locus_tag="VYN69_001789"
     CDS             complement(358..909)
                     /locus_tag="VYN69_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1534014.1"
                     /translation="MKSIGTQILQTERLILRRFVESDAEAMFQNWASSTENLTYVTWD
                     PHPDVEITRNSIRNWVASYTNLNYYKWAICLKENPEQVIGDISIVKIDEADLSCEIGY
                     VLGKAYWGHGMMTEALKAVLDFCFTQAGFQKVRARYASLNPASGRVMEKAGMSYLQTI
                     VNGVERKGYLADLIYYGISREEC"
     gene            complement(1259..2083)
                     /gene="nadE"
                     /locus_tag="VYN69_001790"
     CDS             complement(1259..2083)
                     /gene="nadE"
                     /locus_tag="VYN69_001790"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000058066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEV1534015.1"
                     /translation="MSLQETIIQELGVKPVIDAQEEIRRSIDFLKRYLKKHPFLKTFV
                     LGISGGQDSTLAGRLAQLAMEELRAETGDDSYKFIAVRLPYGVQADEADAQKALAFIQ
                     PDVSLVVNIKESADAMTAAVEATGSPVSDFNKGNIKARCRMIAQYALAGSHSGAVIGT
                     DHAAENITGFFTKFGDGGADILPLYRLNKRQGKQLLQKLGAEPALYEKIPTADLEEDK
                     PGLADEVALGVTYAEIDDYLEGKTISPEAQATIENWWHKGQHKRHLPITVFDDFWE"
     gene            complement(2080..3540)
                     /locus_tag="VYN69_001791"
     CDS             complement(2080..3540)
                     /locus_tag="VYN69_001791"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016466109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEV1534016.1"
                     /translation="MYPDDSLTLHTDLYQINMMQVYFDQGIYNKKAVFEVYFRQQPFK
                     NGYAVFAGLERIVNYLEDLRFSDSDIAYLESLGYHGAFLDYLRNFKLELTVRSAQEGD
                     LVFANEPIVQVEGPLAQCQLVETALLNIVNYQTLVATKAARIRSVIEDEPLMEFGTRR
                     AQEMDAAIWGTRAAVIGGANGTSNVRAGKLFDIPVLGTHAHALVQVYGNDYEAFKAYA
                     ATHKNCVFLVDTYDTLRIGVPAAIQVARELGDQINFMGVRIDSGDIAYISKKVRQQLD
                     EAGFPEAKIYASNDLDENTILNLKMQKAKIDVWGVGTKLITAYDQPALGAVYKIVAIE
                     DENGQMRNTIKLSNNAEKVSTPGKKQVWRITSREKGKSEGDYITYDGVDISDMTEIKM
                     FHPTYTYIKKTVRNFDAVPLLVDIFKEGILVYNLPSLTDIQDYARKEFDKLWDEYKRV
                     LNPQHYPVDLARDVWQDKMDLIDKMRKEALGEGEEE"
     gene            complement(3656..4144)
                     /locus_tag="VYN69_001792"
     CDS             complement(3656..4144)
                     /locus_tag="VYN69_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534017.1"
                     /translation="MKNRFYYYQLLDEREEQLFNKAGSESFYICIALSLLSYIISVLA
                     PSLFNSNMLLIVIIIGTFYFFNRARYLGVTYYSRFHFTILGCFFLTLAITALLMLQNY
                     QFNIEIYQHNPLNFKYLSAWVITYVIYLPWVFIGNLGLKSYGEWAQKKFEQDMDELES
                     GE"
     gene            complement(4134..4343)
                     /locus_tag="VYN69_001793"
     CDS             complement(4134..4343)
                     /locus_tag="VYN69_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013670190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1534018.1"
                     /translation="MAKNLKLKLARVERDLTQGQLAEAVGVTRQTIGLIEAGKYNPSL
                     SLCQSICRCLGKTLDQLFWEEEDEK"
     gene            complement(4729..5181)
                     /locus_tag="VYN69_001794"
     CDS             complement(4729..5181)
                     /locus_tag="VYN69_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000640911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534019.1"
                     /translation="MIYVLSNFLYAISVSIIYALNGIVLLVIVSKLGIPGDLGLDFIV
                     AIVVNTILLVLFYFLLSYIFYLYKLKSGLVFGILVALLLFIPNILNTMMMNTSNDLFI
                     KAIELLPFYSLPVFVASNTMSISQYLVVITTIILLYFFTLKKSKKYSF"
BASE COUNT         1669 a         1183 c          973 g         1627 t
ORIGIN      
        1 catcagaatg agactatttt aatattagat aatttgatag aaaaaaacat aaataaaatt
       61 ggttgaattt aattccctaa ctcgatagag gattctattt taaatttaac aacagctagg
      121 attacactat aaaaagtaca ttaccacctg ttcggctgga gaatctagtc gattagctcc
      181 ctcttagtta caagcttaaa gcgatttttt aactaatact aaaccagatt aaagaaggaa
      241 ctagaacatc tgtcttgtac taatcaatat gctattttaa aattgcaaat aaacttggaa
      301 tgctaattat tatatttaca ataaatagtt gacttcgata gaaacagaaa atagaattca
      361 acattcttcc ctacttatac cataataaat aagatccgca agatagcctt ttctctctac
      421 accattaaca atggtttgta gataggacat tccagccttt tccatgacac gacctgaagc
      481 tgggttgaga ctggcataac gtgctctgac cttttgaaaa cctgcttgag taaaacaaaa
      541 gtccaagaca gctttcaagg cctctgtcat cataccatgt ccccagtaag ccttgcctag
      601 cacatagccg atttcacagc ttaaatcagc ctcgtctatc ttaacgatgc tgatatctcc
      661 tatcacttgc tctgggtttt cttttagaca aatggcccat ttgtaatagt tgagattagt
      721 ataggaagca acccaattgc gaatcgagtt tcgagtgatt tcgacatcag gatggggatc
      781 ccaggtaaca taggtcagat tctcagtgga tgaagcccaa ttttgaaaca tggcttctgc
      841 atcactctcc acaaatcttc ttaaaatcaa acgttctgtc tgtaatattt gcgtaccgat
      901 agatttcatg atgctacctc acttccttat ttctatactc gtagtaaaaa ggctcaactc
      961 agtaacagct agaaaacgag tttgtcttct aatttcttga cgcagttgct gcctggtttg
     1021 aaatgcgctc tagcaagctt tttcaaacct agtcatcgtt gcaggggtaa gacgatgaaa
     1081 tcgaactctt cgagttactg aatctgcctc attttcttat ttctaaactc ggggttcaag
     1141 atcccccaga ccttctctca ctttcttatt tcaagatgag aggctgaaaa cctccagtgg
     1201 aggtttttct tgctttttaa tttcagggca agaagctaaa aaggtccccc gaaccttttt
     1261 actcccaaaa gtcatcaaat acggtgatgg gtaagtggcg tttgtgttgg cctttgtgcc
     1321 accagttttc aatggtcgct tgagcttctg ggctgattgt tttgccttct aggtagtcgt
     1381 caatctctgc gtaggtgact ccaagtgcga cttcgtcagc taggcctggt ttatcttctt
     1441 ctaggtctgc cgttgggatt ttttcataaa gggctggctc tgcgccaagt ttctgcaaga
     1501 gctgttttcc ttggcgttta ttgaggcggt aaagagggag aatatccgca ccgccgtcac
     1561 caaacttggt aaagaaacct gtgatatttt ccgcggcgtg gtctgttcca atgaccgctc
     1621 cgctatggga accagcaagg gcatactgag caatcatacg gcaacgtgcc ttgatattcc
     1681 ccttgttgaa gtctgaaaca ggactacctg tcgcttcaac tgcagctgtc atggcatcag
     1741 ctgattcctt gatattcaca accaagctga catctggctg gatgaaggct agggcttttt
     1801 gagcatctgc ttcatcagct tgcactccgt atggcaggcg gacagcgata aatttgtagc
     1861 tatcgtctcc cgtttcagct cgcagttctt ccatagctaa ttgcgccaaa cgtcctgcca
     1921 aggttgagtc ttgtccccca gaaatcccta gtacaaaggt ttttaggaag ggatgttttt
     1981 tcagatatct ttttaagaaa tcaatagaac gacggatttc ttcctgggca tcaatcactg
     2041 gtttgacacc cagctcttgg ataatcgttt cttgcaaact cattcttctt ctccttcacc
     2101 aagggcttcc ttgcgcatct tatcaatcaa gtccatctta tcttgccata catcacgcgc
     2161 caaatccact ggatagtgct gcggattgag cacacgctta tactcatccc acaacttgtc
     2221 aaattcctta cgggcataat cctgaatgtc agtcaaacta ggcaagttgt aaactaatat
     2281 tccttctttg aagatatcca ccaagagagg aacggcatca aaattacgaa ccgtcttctt
     2341 gatgtatgta taggtcggat ggaacatctt aatttctgtc atgtcgctaa tatccacacc
     2401 atcataagtg atgtagtcac cttctgactt gcctttttca cgactggtaa tgcgccacac
     2461 ctgcttctta cctggcgtcg acactttttc cgcattatta gacagcttaa tcgtattgcg
     2521 catctggccg ttttcatctt cgattgcaac aatcttgtaa accgccccaa gagccggctg
     2581 gtcataggct gtaatcagct tggtacccac accccagaca tcaatcttgg ccttttgcat
     2641 cttgaggtta aggatggtat tttcatctag atcattagaa gcataaatct tagcctctgg
     2701 aaatccagcc tcgtccagtt gctgacggac tttcttagaa atgtaggcaa tatccccaga
     2761 gtcaatccgc acacccataa agttaatctg atcacccagc tcacgcgcca cctgaatggc
     2821 agctggtaca ccgatgcgaa gggtatcata ggtatccaca agaaagacac aatttttgtg
     2881 ggtcgcagcg taagccttga aagcctcata gtcattgcca taaacctgta ccaaggcatg
     2941 ggcatgggtt cccaaaacag gaatgtcaaa gagcttaccc gcacgcacgt tgctggttcc
     3001 attggcgcca ccaatcaccg ctgcgcgtgt tccccagatg gccgcatcca tttcttgagc
     3061 ccgacgtgtc ccaaactcca tcaagggttc atcttcgata accgaacgaa tacgagctgc
     3121 cttcgtcgcc accaaggtct ggtagttgac gatgttcaaa agagccgttt cgaccaactg
     3181 acattgggct agaggtcctt ccacctgcac aatcggttca ttagcaaaaa ccaaatcccc
     3241 ttcttgggca gaacgaacgg tcaactccaa cttgaaattg cgaaggtaat ccaagaacgc
     3301 cccatgataa ccaagcgact ccaaataggc tatatcacta tctgaaaaac gcaagtcttc
     3361 aagatagttc acaattcttt ccaaacctgc aaaaaccgca tagccgttct taaaaggctg
     3421 ttggcggaaa tacacctcaa agaccgcctt cttattgtaa atcccttgat caaagtaaac
     3481 ctgcatcatg ttgatctggt acaagtccgt gtgcaatgtc aaactatcat ctggatacat
     3541 acttttccta cttccttagc tagaaaccca tgaaaatttt caagaacttt catgtattcc
     3601 aataaattag tactattata tcacatttta gctggattga gaaaagagta acaagctatt
     3661 ctccactctc caattcatcc atatcttgtt caaatttttt ctgagcccat tcgccatagc
     3721 tcttaagacc aagattgcca ataaagaccc acggaaggta aatgacataa gtaatgaccc
     3781 aagcagacag gtatttaaaa ttcaaaggat tgtgctgata aatttctatg ttgaattgat
     3841 aattctgcaa catcaaaaga gccgtaatag ccaaggttag gaaaaaacaa cccaaaatcg
     3901 taaaatgaaa acgactatag taggtcactc ccagataacg ggcacgattg aaaaagtaaa
     3961 atgtccctat gatgataacg attagcagca tattagaatt aaaaaggctt ggtgctaata
     4021 ctgaaatgat ataagatagg agcgacaaag cgatgcagat atagaaactt tcagagcccg
     4081 ctttattgaa cagttgttct tctctttcgt ctagtaattg ataataataa aatctatttt
     4141 tcatcttctt cctcccaaaa tagttggtct agggttttcc ctaaacatct gcaaatggac
     4201 tggcagagcg agagactggg attgtatttc cccgcttcta tcaaaccaat agtctggcgc
     4261 gtcaccccga cagcctctgc cagttgacct tgtgttaaat cacgctctac ccgagctaat
     4321 tttaatttta aatttttagc caccttcgtc ctccttatag ttttaatact catctactct
     4381 taaaaaatcc aaaaccaaca caagctatca gctatttatt ataaccccat taatttgcac
     4441 tccagtatta tcttcagccg aaaatcagta ttttaggttg tttgaaataa acttcctagt
     4501 ttactctttt atttttattt agtattagct ttagtaggat cttttaaacg agtttttatt
     4561 gcttatattt cttgtttagt ttagttggtt tcatatcact attatataat gcttttggaa
     4621 atgagcctag tataagtcaa aaacgtgttg aatagtgaaa gcaggagcat accaagatga
     4681 ctgatttttt tattgtattt taggtatgca aaataatact aaatgaaact aaaatgaata
     4741 cttcttgctt ttcttgagag tgaaaaagta caataaaatg attgtagtga ttaccacaag
     4801 atactgacta atagacatcg tatttgaagc cacaaataca ggtaaagaat aaaaaggaag
     4861 aagttcaatt gctttgataa acaaatcatt actagtattc atcatcatcg tatttaatat
     4921 attagggata aagagtagta aagctactaa aataccaaat accaagccac ttttcaattt
     4981 gtataaataa aaaatgtaag ataatagaaa ataaaacagg actaacaaaa ttgtattgac
     5041 tacaatagct actataaaat ctaatcctaa atcacctgga atacccaatt tacttactat
     5101 gactagtaac acaatgccat tcaaagcata aataatggaa actgagatgg catatagaaa
     5161 attagacaag acgtaaatca tccgattctt ctgttgatta taaaacaaac ttaccgtatt
     5221 gtgggaaaaa tctctactaa tgactcgatt agcatgtata acagccatta acataccgag
     5281 catagcataa aagtttgaaa tatgcttaat accaagcgat gtcccttaag tctttagaat
     5341 aaagaatgta accaatatcg gcggtataaa cgagataagc aaaccgagat atattacgcc
     5401 tgatgaatat aaatttctta tattttcctt aagaaaattc aatttattta at
//
LOCUS       DAVLVP010000102         5424 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.102, whole genome shotgun sequence.
ACCESSION   DAVLVP010000102 DAVLVP010000000
VERSION     DAVLVP010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.102"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            137..895
                     /locus_tag="VYN69_001795"
     CDS             137..895
                     /locus_tag="VYN69_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEV1534020.1"
                     /translation="MALVEFKNVEKYYGDYHAFRNINLRFEKGQVVVLLGPSGSGKST
                     LIRTINGLETVDKGSLLVNGHQVAGASQKDLVPLRKEVGMVFQHFNLYPHKAVLENVT
                     LAPIEVLGIDKKEAEKTAQKYLEFVNMWDKKDSYPAMLSGGQKQRIAIARGLAMHPEL
                     LLFDEPTSALDPETIGDVLAVMQKLAHDGMNMIIVTHEMGFAREVADRIIFMADGEVL
                     VDTTDVDNFFDNPSEPRAQQFLSKIINHESDKVK"
     gene            935..1699
                     /locus_tag="VYN69_001796"
     CDS             935..1699
                     /locus_tag="VYN69_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000624332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEV1534021.1"
                     /translation="MISLFGLAAAKPVQADTSIADIQKRGELVVGVKQDVPNFGYKDP
                     KTGTYSGIETDLAKMVADELKVKIRYVPVTAQTRGPLLDNEQVDMDIATFTITDERKK
                     LYNFTSPYYTDASGFLVNKSAKIKKIEDLNGKTIGVAQGSITQRLITELGKKKGLKFK
                     FVELGSYPELITSLHAHRIDTFSVDRSILSGYTSKRTALLDDSFKPSDYSIVTKKSNT
                     ELNDYLDNLVTKWSKDGSLQKLYDRYKLKPSSHTAD"
     gene            1712..2389
                     /locus_tag="VYN69_001797"
     CDS             1712..2389
                     /locus_tag="VYN69_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1534022.1"
                     /translation="MTDLSSWTAYFQDFGQFFNGFLFTLALAVGSFILAMVLGIFFGA
                     MSTSKRPILRILARIFVEFYQNTPLLVQFVIVFYGLPLISDHIIMIPIYWTAVLCVGL
                     YHGAYIAEVIRSGIQSIPSGQMEAALSQGFTYISAMRLITLPQAFRIILPPLTNQIVN
                     LIKNTSTVAIISGVDLMFVTKSWSALNGNYIPAFLGAALLYFALCFPVAQFGRKMEQA
                     NKKAYSL"
     gene            2399..3058
                     /locus_tag="VYN69_001798"
     CDS             2399..3058
                     /locus_tag="VYN69_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEV1534023.1"
                     /translation="MESILEVLTPDNLVFIFKGFGLTLYISLIAIILSTIIGTVLAVM
                     RNGKNPILRIISSIYIEFVRNVPNLLWIFTIFLVFKMKSTPAGITAFTLFTSAALAEI
                     IRGGLNAVDKGQYEAGMSQGFTSAQILYYIILPQAIRKMLPAIISQFVTVIKDTSLLY
                     SVIALQELFGASQILMGRYFEPEQVFSLYILIALIYFSFNLAISSLSHMLAKRWQQAA
                     E"
     gene            3316..4506
                     /locus_tag="VYN69_001799"
     CDS             3316..4506
                     /locus_tag="VYN69_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HEV1534024.1"
                     /translation="MKSVKGLLFIIASFILTLLTWMNTSPQFMIPGLALTSLSLTFIL
                     ATRLPLLESWFHSLEKVYTVHKFTAFLSIILLIFHNFSMGGLWGSRLAAQFGNLAIYI
                     FASIILVAYLGKYIQYEAWRWIHRLVYLAYILGLFHIYMIMGNRLLTFNLLSFLVGSY
                     ALLGLLAGFYIIFLYQKISFPYLGKITHLKRLNHDTREIQIHLSRPFNYQSGQFAFLK
                     IFQEGFESAPHPFSISGGHGQTLYFTVKTSGDHTKNIYDNLQAGSKVTLDRAYGHMII
                     EEGRENQVWIAGGIGITPFISYIREHPILDKQVHFYYSFRGDENAVYLDLLRNYAQKN
                     PNFELHLIDSTKDGYLNFEQKEVPEHATVYMCGPISMMKALAKQIKKQNPKTELIYEG
                     FKFK"
     gene            4956..>5424
                     /locus_tag="VYN69_001800"
     CDS             4956..>5424
                     /locus_tag="VYN69_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEV1534025.1"
                     /translation="MEQLHFITKLLDIKDPNIKILDIINMDTHKEIIAKLDYEAPSCP
                     DCGSLMKKYDFQKPSKIPYLETTGMPSRILLRKRRFKCYHCSKMMVAETPLVKKNHQI
                     PRIINQKIAQKLIEKISMTDIAHQLAISTSTVIRKLNDFHFEHDFSRLPKIMSW"
BASE COUNT         1593 a         1231 c          960 g         1640 t
ORIGIN      
        1 agaaatcgat ttgactgtcc cgatcgattt ttcctgttct tatttcacta tagtgaattt
       61 tttcagatat gatttgacaa gaacattctt ttattgtaga accgtgttat aaaatataac
      121 acaatagaga tctctcatgg ctttagtaga atttaaaaac gtcgaaaaat attacggaga
      181 ctaccacgca ttccgcaaca tcaatctccg ttttgaaaaa ggacaagttg ttgtcctgct
      241 tggaccttct ggctctggga agtccactct tatccgtacg atcaatggtt tagagactgt
      301 tgacaaagga agtctcctag tcaatgggca ccaagttgct ggtgccagcc agaaagattt
      361 ggtacctctt cgcaaggaag tcggcatggt ttttcaacat tttaaccttt atccacacaa
      421 agctgtgtta gaaaacgtaa cgcttgcacc cattgaagtt ctaggaattg ataaaaaaga
      481 agctgaaaaa accgcccaaa aatatctgga atttgtaaat atgtgggaca agaaagattc
      541 ctatcccgcc atgctatctg gtggacaaaa acagcggatc gccatcgctc gtggtcttgc
      601 tatgcatccg gaactcctcc tctttgatga accaacatct gctcttgatc ctgagactat
      661 cggagatgtt ctagcagtta tgcagaaact ggcgcatgat gggatgaaca tgatcatcgt
      721 tacccacgaa atgggctttg ctcgagaggt tgcggaccgc attatcttta tggccgacgg
      781 agaagtttta gtagatacga cagatgtcga taactttttt gacaatccaa gcgaacctcg
      841 tgcccaacaa ttcctcagca aaattatcaa ccacgaaagt gacaaagtca aataaggagg
      901 cgcctatgaa aaaaaattct ttttatcagc attattgatt agccttttcg gccttgctgc
      961 tgccaaacca gtccaggctg atacaagtat cgcagacatt caaaaaagag gcgaactggt
     1021 tgtcggtgtc aaacaagacg ttcccaattt tggttacaaa gatcccaaga ccggtactta
     1081 ttctggtatc gaaaccgact tggccaagat ggtagctgat gaactcaagg tcaagattcg
     1141 ctatgtgccg gttacagcac aaacccgcgg cccccttcta gacaatgaac aggtcgatat
     1201 ggatatcgcg acctttacca tcacggacga acgcaaaaaa ctctacaact ttaccagtcc
     1261 ctactacaca gacgcttctg gatttttggt caataaatct gccaaaatca aaaagattga
     1321 ggacctaaac ggcaaaacca tcggagtcgc ccaaggttct atcacccaac gcctgattac
     1381 tgaactgggt aaaaagaaag gtctgaagtt taaattcgtc gaacttggtt cctacccaga
     1441 attgattact tccctgcacg ctcatcgtat cgataccttt tccgttgacc gctctattct
     1501 atctggctac actagtaaac ggacagcact actagatgat agtttcaagc catctgacta
     1561 cagtattgtt accaagaaat caaatacaga gctcaacgac tatcttgata acttggttac
     1621 taaatggagc aaggatggta gtttgcagaa actttatgac cgttacaagc tcaaaccatc
     1681 tagccatact gcagattaag gaggacaccc catgacagat ttatcatctt ggacagccta
     1741 ttttcaggat tttggacaat ttttcaatgg tttcctcttc acccttgccc tagcggttgg
     1801 ttcctttatc ctcgccatgg tcttaggaat cttctttggt gccatgtcaa ccagtaaacg
     1861 tccaatttta cgcattttag ctcgcatctt tgttgaattt taccaaaata ctcctctctt
     1921 ggtgcagttt gttattgttt tttatggcct acctcttatc agtgaccata tcatcatgat
     1981 tccaatttat tggacagccg ttctctgcgt gggactctat cacggcgctt atatcgctga
     2041 ggttattcgt tcagggattc agtctattcc tagtggtcag atggaggccg ccttgtcgca
     2101 aggttttacc tatatcagtg ccatgcgctt gattaccttg cctcaggcct tccgcatcat
     2161 tctccctcca ttgaccaacc aaattgttaa cctcatcaag aacacatcta ctgtagctat
     2221 catctctgga gtagacttga tgtttgtgac taagtcttgg tcggctctca acggaaacta
     2281 tatcccagcc tttttaggtg ctgctcttct ctactttgcc ctatgcttcc ctgttgccca
     2341 gtttggtcgc aagatggagc aagccaacaa aaaagcctat tcactttagg aggttactat
     2401 ggaatctatt ttagaagttt taaccccaga taacctagtc tttatattta aaggatttgg
     2461 cttgaccctc tatatttctc tgattgccat catcctctct actatcatcg gtaccgtgct
     2521 tgccgtcatg cgaaatggaa aaaatcctat cttacgcatt atttccagta tttatatcga
     2581 gtttgtgcgc aacgttccta accttctctg gatttttact atctttttgg tgttcaaaat
     2641 gaaatccaca ccagcaggta ttacagcctt tactctcttt acatcagcag ccttggctga
     2701 gattattcga ggcggtctca atgccgtaga caagggacaa tacgaagcag gaatgtcaca
     2761 aggcttcacc tcagcccaaa tcctctacta catcattctc ccacaagcca tccgcaaaat
     2821 gctaccagcc atcatttctc agtttgttac cgtgattaag gataccagtc tcctctactc
     2881 tgttatcgcc ctacaagaac tctttggagc cagccaaatt ctcatgggcc gttatttcga
     2941 accagagcag gtcttcagtc tttacatcct gattgccctc atctacttca gctttaacct
     3001 agcaatttct agcctgtctc atatgctagc caaacgttgg caacaagctg cagaataaca
     3061 ctcttcgaaa atctcttcaa accacgtcag ctctatctgc aacctcaaaa cagtgttttg
     3121 agcaacttgc ggctagcttc ctagtttgct ctttgatttt cattgagtat aaacagccac
     3181 tctccagttc aattggagag ttttttgatg agagtagata tttatagacc tcaatttgac
     3241 accatacatt ttctatgata aaatgtaaca aatttattta caacaaatcg aaatctaaat
     3301 gaaatggaat tttctatgaa atcagtcaaa ggactactct ttatcatagc tagttttatc
     3361 ttgactcttt tgacttggat gaacacttct ccccaattca tgattccagg actagcttta
     3421 acaagcctat ctctgacttt tatcctagcc actcgtctcc cactactaga aagctggttt
     3481 cacagtttgg agaaggtcta caccgtccac aaattcacag cctttctctc aatcatccta
     3541 ctaatctttc ataactttag tatgggcggt ttgtggggct ctcgcttagc tgctcagttt
     3601 ggcaatcttg ccatctatat ctttgccagc atcatccttg tcgcctattt aggcaaatac
     3661 atccaatacg aagcttggcg atggattcac cgcctggttt acctagccta tattttagga
     3721 ctctttcaca tctacatgat aatgggcaat cgtctcctta catttaatct tctaagtttt
     3781 cttgttggta gctatgccct tttaggctta ctagctggtt tttatatcat ttttctatat
     3841 caaaagattt ccttccccta tctagggaaa attacccatc tcaaacgctt aaatcacgat
     3901 actagagaaa ttcaaatcca tcttagcaga cctttcaact atcaatcagg acaatttgcc
     3961 tttctaaaga ttttccaaga aggctttgaa agtgctccgc atcccttttc tatctcagga
     4021 ggtcatggtc aaactcttta ctttactgtt aaaacttcag gcgaccatac caagaatatc
     4081 tatgataatc ttcaagccgg cagcaaagta accctagaca gagcttacgg acacatgatc
     4141 atagaagaag gacgagaaaa tcaggtttgg attgctggag gtattgggat cacccccttc
     4201 atctcttaca tccgtgaaca tcctatttta gataaacagg ttcacttcta ctatagcttc
     4261 cgtggagatg aaaatgcagt ctacctagat ttactccgta actatgctca gaaaaatcct
     4321 aattttgaac tccatctaat cgacagtacg aaagacggct atcttaattt tgaacaaaaa
     4381 gaagtgcccg aacatgcaac cgtctatatg tgtggtccta tttctatgat gaaggcactt
     4441 gccaaacaga ttaagaaaca aaatccaaaa acagagctta tttacgaagg attcaagttc
     4501 aaataagtaa tcatactctc ctggaatttc ccaggagagt tttttatagc caaatgaaat
     4561 aagacaggaa cgatggaatt ttggcattca aatcaattga taagaatatt ttatagtgga
     4621 gtgaaatagg atacgaacaa attgattagg aaaatcaaat gaatttatag aaatctttta
     4681 gcagttatgt tgtcctattc tagtttcaaa acgctataga agcagcattg tgctagtcta
     4741 gattcagttt actatactaa aacgagtagc ttgaaatcaa aaaacccacc ctcacaggca
     4801 ggttttatct gtattattca gctagattat gcggctctat aatatttgta gtgggtaaat
     4861 ccactataga tattatggag cctattttat tgtagaaaaa aagtcccata tgacctataa
     4921 tgaaaagcga caaaaccatc attagaaaga ttcatatgga acaattacat tttatcacaa
     4981 aactgctcga tattaaagac ccaaacatca agattctaga tatcatcaat atggataccc
     5041 acaaagaaat tatcgctaag ctggattatg aggctccatc ttgccctgat tgtggaagtc
     5101 taatgaagaa atatgacttt caaaaaccgt ctaagatccc ttacctcgaa acaactggta
     5161 tgccttctag aattctcctt agaaaacgcc gtttcaagtg ctatcactgt tcaaaaatga
     5221 tggttgctga gactcccctg gtaaagaaaa atcaccaaat ccctcgtatc atcaaccaaa
     5281 aaattgctca aaagttaatt gaaaaaattt ctatgactga tattgcccat cagctggcca
     5341 tttcaacttc aactgtcatt cgcaagctca atgactttca ctttgagcat gatttttctc
     5401 ggcttccaaa gattatgtct tggg
//
LOCUS       DAVLVP010000103         5211 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.103, whole genome shotgun sequence.
ACCESSION   DAVLVP010000103 DAVLVP010000000
VERSION     DAVLVP010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 5211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5211
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.103"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            87..1262
                     /gene="mutY"
                     /locus_tag="VYN69_001801"
     CDS             87..1262
                     /gene="mutY"
                     /locus_tag="VYN69_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEV1534026.1"
                     /translation="MLDLKEYGIVMWPEEKVISFREKLLAWYDENKRDLPWRRSKNPY
                     HIWVSEIMLQQTRVDTVIPYYERFLDWFPTVESLATAPEESLLKAWEGLGYYSRVRNM
                     QAAAQQIMTDFGGQFPNTYEGISSLKGIGPYTAGAISSIAFNLPEPAVDGNVMRVLAR
                     LFEVNHDIGIPSNRKIFQAMMEILINPDRPGDFNQALMDLGSDIESPVNPRPEESPVK
                     DFSAAYQNGTMDRYPIKSPKKKPVPIYLKALVVKNSQGQFLLEKNESEKLLAGFWHFP
                     FIEVDNFSQEEQFDLFHQVAEESVNSGPSPEESFQQDYDLDVDWLDVCFDTVQHVFSH
                     RKWHVQIVAGQVSDFHDFSDREVRWLSPEEFKNYPLAKPQQKIWQAYAQANLDSSQD"
     gene            1317..2021
                     /locus_tag="VYN69_001802"
     CDS             1317..2021
                     /locus_tag="VYN69_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1534027.1"
                     /translation="MKKILIVDDEKPISDIIKFNMTKEGYEVVTAFNGREALEQFEAE
                     QPDIIILDLMLPEIDGLEVAKTIRKTSSVPILMLSAKDSEFDKVIGLELGADDYVTKP
                     FSNRELQARVKALLRRSQPMPVDGQEADSKPQPIQIGDLEIVPDAYVAKKYGEELDLT
                     HREFELLYHLASHTGQVITREHLLETVWGYDYFGDVRTVDVTVRRLREKIEDTPSRPE
                     YILTRRGVGYYMRNNA"
     gene            2014..3363
                     /gene="vicK"
                     /locus_tag="VYN69_001803"
     CDS             2014..3363
                     /gene="vicK"
                     /locus_tag="VYN69_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000886221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     VicK"
                     /protein_id="HEV1534028.1"
                     /translation="MLDLLKQTIFTRDFIFILILLGFILVVTLLLLENRRDNIQLKQI
                     NQKVKDLIAGDYSKVLDMQSGSEITNITNNLNDLSEVIRLTQENLEQESKRLNSILFY
                     MTDGVLATNRRGQIIMINDTAKKQLGLVKEDVLNRSILELLKIEENYELRDLITQSPE
                     LLLDSQDINGEYLNLRVRFALIRRESGFISGLVAVLHDTTEQEKEERERRLFVSNVSH
                     ELRTPLTSVKSYLEALDEGALCETVAPDFIKVSLDETNRMMRMVTDLLHLSRIDNATS
                     HLDVELINFTAFITFILNRFDKMKGQEKEKKYELVRDYPINSIWMEIDTDKMTQVVDN
                     ILNNAIKYSPDGGKITVRMKTTEDQMILSISDHGLGIPKQDLPRIFDRFYRVDRARSR
                     AQGGTGLGLSIAKEIIKQHKGFIWAKSEYGKGSTFTIVLPYDKDAVKEEVWEDEVED"
     gene            3365..4174
                     /locus_tag="VYN69_001804"
     CDS             3365..4174
                     /locus_tag="VYN69_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEV1534029.1"
                     /translation="MSEIGFKYSILASGSSGNSFYLETSKKKLLVDAGLSGKKITSLL
                     AEINRKPEDLDAILITHEHSDHIHGVGVLARKYGMDLYANEKTWQAMENSKYLGKVDS
                     SQKHIFEMGKTKTFGDIDIESFGVSHDAVAPQFYRFMKDDKSFVLLTDTGYVSDRMAG
                     IVENADGYLIEANHDVEILRSGSYAWRLKQRILSDLGHLSNEDGAEAMIRTLGNRTKK
                     IYLGHLSKENNIKELAHMTMVNQLAQADLGVGVDFKVYDTSPDTATPLTEI"
     gene            4265..4507
                     /locus_tag="VYN69_001805"
     CDS             4265..4507
                     /locus_tag="VYN69_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001853822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEV1534030.1"
                     /translation="MTTITLKVSEADKTFMKAMAKFEGVSLSELIRTKTLEALEDEYD
                     ARVADLAYQEYLEDLEKGVEPITWEEMMHDLGLKDE"
     gene            4509..4763
                     /locus_tag="VYN69_001806"
     CDS             4509..4763
                     /locus_tag="VYN69_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEV1534031.1"
                     /translation="MYKLVPTRRFIKQLKKLDRYTQKLITNYLQINVLEDPRRHGKAL
                     VGNRVGQWRYRIGNYRVIVQIVDDELVVATLEVGHRRDIY"
     gene            4810..>5211
                     /locus_tag="VYN69_001807"
     CDS             4810..>5211
                     /locus_tag="VYN69_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEV1534032.1"
                     /translation="MKKKWFFADYYDTTIILLALISVILVLLGFAEMIDLDNPPYSII
                     DLVIWGVFVIDYSWRFFITKRKWRFILENVFDLLAILPLNAIFTVFRLGRIFRLVKLT
                     KLLKLTRLLRIIGLTGKLERKISRFLRTNGLI"
BASE COUNT         1605 a          877 c         1171 g         1558 t
ORIGIN      
        1 tagatagaaa agttatatac tatctattga aaatattata aatcagatac aaagacaggt
       61 gaaaccttct gtgttatact agagatatgt tagatttgaa agaatacggt atcgtcatgt
      121 ggccggagga gaaagtcatt tctttccgtg agaaacttct cgcttggtat gatgaaaaca
      181 aaagagattt gccttggagg agaagtaaaa atccttatca catctgggta tctgaaatca
      241 tgcttcagca gaccagggtg gatacagtta tcccttacta cgaaagattt ttggactggt
      301 ttccaactgt cgaaagtctg gcaactgcgc ctgaggagag tttactgaaa gcttgggagg
      361 gcttgggtta ttattctcga gttcgcaata tgcaggctgc agcccagcaa attatgactg
      421 actttggtgg ccaatttcca aatacctatg aaggaatttc cagcttgaaa gggattggac
      481 cttacacagc aggagccatt tccagtattg cttttaactt gcctgagcca gctgtagatg
      541 gtaatgtcat gcgggtcttg gcgcgtctgt ttgaagtcaa ccacgatatt gggattccaa
      601 gtaatcgaaa aatttttcag gcaatgatgg agatcttgat taatccagat cgtccgggtg
      661 attttaatca agccttgatg gacttagggt ctgatattga gtctcctgta aatcccagac
      721 ctgaagaaag cccagtcaag gactttagtg cggcatatca gaatggaacc atggaccgtt
      781 atccaattaa atctcctaag aaaaagccgg ttcctattta tcttaaagcc ttagttgtga
      841 aaaattctca aggacaattt ttacttgaaa aaaatgaaag cgaaaagctc ttggcaggtt
      901 tttggcattt cccctttata gaagttgata acttttcgca agaagagcag tttgacctct
      961 ttcatcaggt tgcggaagaa agtgtgaact ctggccccag tccagaagag agtttccagc
     1021 aggactatga cctagatgtt gattggcttg atgtttgttt tgatacagtc cagcatgttt
     1081 ttagtcatcg aaaatggcat gtacagattg tagcaggtca ggtgagtgac ttccatgatt
     1141 tttcagatag ggaagttcgt tggctttcac cagaagaatt caagaattac ccacttgcca
     1201 aaccccaaca aaaaatctgg caggcttatg cacaagccaa cttagacagt agccaagact
     1261 agctattgtg tcttttgact attttttggt ataatagtag agaaaaaggt gaacatatga
     1321 aaaaaatact aattgtagat gatgagaaac caatctcgga tattatcaag tttaatatga
     1381 ccaaggaagg ttacgaagtt gtaactgctt ttaatggtcg tgaagcgcta gagcaatttg
     1441 aagcagagca accagatatt attattctgg atttgatgct tccagaaatt gatggtttag
     1501 aagttgctaa gaccattcgt aagacaagca gtgtgcccat tcttatgctt tcagccaaag
     1561 atagtgaatt tgataaggtt atcggtttgg aacttggggc agatgactat gtaacgaaac
     1621 ccttctccaa tcgtgagttg caggcgcgtg ttaaagctct tctgcgtcgt tctcaaccta
     1681 tgccagtaga tggtcaggaa gcagatagta aacctcaacc tatccaaatt ggggatttag
     1741 aaattgttcc agacgcctac gtggctaaaa aatatggcga agaactagac ttaacccatc
     1801 gtgaatttga gcttttgtat catttagcat cgcatacagg tcaagtcatc acgcgcgaac
     1861 acttgcttga gactgtctgg ggttatgact attttggtga tgtccgtaca gttgatgtga
     1921 ctgtacgacg tctgcgtgag aagattgaag atacgcccag ccgaccagag tatatcttga
     1981 cgcgccgtgg tgtagggtat tacatgagaa ataatgcttg atttactgaa acaaaccatt
     2041 tttaccagag attttatctt tatcctgatt ttgttaggtt tcatccttgt tgtgaccctc
     2101 ttattactgg aaaatagacg tgataatatt cagttgaagc aaatcaatca aaaggttaaa
     2161 gatttgattg caggagatta ttccaaggtt cttgatatgc aaagtgggtc tgaaatcacc
     2221 aatattacca ataatttgaa tgacttgtcg gaggttattc gtctcactca ggaaaatcta
     2281 gaacaagaga gtaagaggct aaatagtatt ctgttttata tgacagatgg ggttcttgcg
     2341 accaaccgtc ggggtcagat tatcatgatt aacgatacag ccaagaagca actggggttg
     2401 gttaaggaag atgttctgaa tagaagcatt ttggaattgc tcaagataga agaaaactat
     2461 gaattgcgtg atttgattac ccaaagtcca gaattgttgc tagattccca agatatcaat
     2521 ggcgaatatt tgaaccttcg agttcgcttt gccttgatac gtcgagagtc tggctttatt
     2581 tcaggtttgg tggctgtttt gcatgatacg acggagcagg agaaggaaga acgcgaacga
     2641 agactctttg tttccaatgt tagccatgag ttacggactc ctctgactag cgtaaaatcc
     2701 tatcttgaag ccttggatga gggggctttg tgtgaaactg tagcaccaga ctttatcaag
     2761 gtttctcttg atgagaccaa ccgtatgatg cgcatggtga cggatctcct ccatctttca
     2821 cgtattgata atgctaccag tcacctagat gtggaactga ttaacttcac tgcttttatt
     2881 acctttatcc tcaatcgttt tgacaagatg aaaggacagg aaaaggagaa aaaatatgag
     2941 ttggtgagag attatcccat caattctatc tggatggaaa ttgatacaga taagatgacg
     3001 caggttgtcg acaatatttt aaataatgct attaagtatt cgccagatgg gggtaaaatc
     3061 actgtcagaa tgaagacaac tgaagaccag atgattttat ccatttctga ccacggtttg
     3121 gggattccta agcaggattt accacgtatc tttgaccgtt tctatcgtgt ggatcgtgct
     3181 agaagtcgtg cacaaggtgg tacaggtcta ggactgtcta tcgctaaaga aattatcaaa
     3241 caacataagg gctttatttg ggccaagagt gaatacggca agggttcaac ctttaccatt
     3301 gtgctccctt atgataagga tgcagtgaaa gaagaagtat gggaggatga agtagaagac
     3361 tagaatgagt gaaataggct ttaaatacag tattttagcg tcgggttcca gtggaaattc
     3421 tttttatctg gaaacctcaa aaaagaagct tttagtagat gcaggcttgt ctggcaagaa
     3481 aattaccagt ctgctagctg aaattaaccg taagccagaa gacctggatg ccatcttgat
     3541 tacccatgag cattcagatc atatccatgg agtaggcgtt ttggctcgca agtatggtat
     3601 ggatctttat gccaatgaaa agacctggca agctatggaa aatagtaaat atcttggcaa
     3661 ggtggattct tcgcaaaagc atatttttga aatgggtaaa accaaaacct ttggagatat
     3721 cgacatcgag agttttggtg taagccatga tgcagtcgca ccgcagttct atcgctttat
     3781 gaaggatgat aagagttttg tccttttgac agatacaggt tatgtcagtg accgtatggc
     3841 gggcattgtc gaaaatgcgg atggctatct tatcgaggcc aaccatgatg tagagatttt
     3901 gcgatcaggt tcttacgctt ggcgactcaa acaacgaatc ctatctgacc ttggtcacct
     3961 ttctaacgag gacggtgctg aagctatgat tcggacgcta ggaaatcgta ctaagaagat
     4021 ttaccttggg catttatcta aggaaaacaa tatcaaggaa ctggctcata tgaccatggt
     4081 caatcagctg gctcaagctg atctgggagt cggagtagac tttaaggttt atgatacctc
     4141 accagatacc gcaacaccat tgacagagat atagaaagaa cgctgagaag gtgttctttt
     4201 tatattgact gaacacctaa aaagtaatac aatggtgtta ccattaaaaa agggagcaca
     4261 aaagatgact actattacat taaaagtttc tgaagctgat aaaacattta tgaaagcaat
     4321 ggctaagttt gaaggagttt ccctttcgga acttattcgc accaaaactc ttgaagccct
     4381 agaagatgaa tacgatgctc gtgtggcaga tttagcctat caagagtatt tagaagactt
     4441 ggaaaaagga gttgaaccca ttacttggga agaaatgatg catgatttag gcttgaagga
     4501 tgaataattt gtataaatta gttccaacaa gacgtttcat caagcaattg aaaaaattgg
     4561 accgttatac gcagaagcta attacaaact atttacaaat caatgttttg gaagacccaa
     4621 gacgacacgg aaaggctttg gttggtaatc gcgttggtca atggcgctat agaattggta
     4681 attatcgagt tatcgtacaa attgtagatg atgaattagt tgttgctact ctagaagttg
     4741 gtcatcggag agatatttat tgaattaaag ctgagtaata acaatataga aagtaaggag
     4801 agtaaaaatt tgaagaaaaa atggtttttt gcagattatt atgatacaac aattatttta
     4861 cttgctttaa tctctgttat tctggtatta cttggatttg ctgagatgat tgatttggac
     4921 aatccgcctt atagtattat tgatttagta atctggggtg tttttgtaat tgattatagt
     4981 tggcgctttt ttattactaa aagaaaatgg cgctttattc ttgaaaatgt ttttgattta
     5041 ttggctattc tccctttaaa tgctattttc acagtttttc gattagggcg tatctttcgt
     5101 ttagttaaac taacaaaatt actgaaactg actcgtttac ttagaataat tggacttaca
     5161 ggtaaattag aaagaaaaat tagtagattc ttacggacaa atggcctgat t
//
LOCUS       DAVLVP010000104         4938 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.104, whole genome shotgun sequence.
ACCESSION   DAVLVP010000104 DAVLVP010000000
VERSION     DAVLVP010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4938)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4938)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4938
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.104"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..319
                     /locus_tag="VYN69_001808"
     CDS             <1..319
                     /locus_tag="VYN69_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000731134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ion transporter"
                     /protein_id="HEV1534033.1"
                     /translation="VVEEKSFSDSLWWALVTVTTVGYGDIVPASIFGKWLAVLLMLVG
                     IGTIGMLTSALTNFFVKDNPDEQIKLDKLKDELSSQRILLEKQSKKIEELHKMIQDLI
                     EKT"
     gene            complement(579..1565)
                     /locus_tag="VYN69_001809"
     CDS             complement(579..1565)
                     /locus_tag="VYN69_001809"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019768616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEV1534034.1"
                     /translation="MTSTKQHKKVILVGDGAVGSSYAFALVNQGIAQELGIIEIPQLH
                     EKAVGDALDLSHALAFTSPKKIYAAQYSDCADADLVVITAGAPQKPGETRLDLVGKNL
                     AINKSIVTQVVESGFKGIFLVAANPVDVLTYSTWKFSGFPKERVIGSGTSLDSARFRQ
                     ALAEKLDVDARSVHAYIMGEHGDSEFAVWSHANIAGVNLEEFLKDTQNVQEAELIELF
                     EGVRDAAYTIINKKGATYYGIAVALARITKAILDDENAVLPLSVFQEGQYGVENVFIG
                     QPAVVGAHGIVRPVNIPLNDAETQKMQASAKELQAIIDEAWKNPEFQEASKN"
     gene            1759..4227
                     /gene="gyrA"
                     /locus_tag="VYN69_001810"
     CDS             1759..4227
                     /gene="gyrA"
                     /locus_tag="VYN69_001810"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEV1534035.1"
                     /translation="MQDKNLVNVNLTKEMKASFIDYAMSVIVARALPDVRDGLKPVHR
                     RILYGMNELGVTPDKPHKKSARITGDVMGKYHPHGDSSIYEAMVRMAQWWSYRYMLVD
                     GHGNFGSMDGDSAAAQRYTEARMSKIALEMLRDINKNTVDFVDNYDANEREPLVLPAR
                     FPNLLVNGATGIAVGMATNIPPHNLGETIDAVKLVMDNPEVTTKDLMEVLPGPDFPTG
                     ALVMGKSGIHKAYETGKGSIVLRSRTEIETTKTGRERIVVTEFPYMVNKTKVHEHIVR
                     LVQEKRIEGITAVRDESNREGVRFVIEVKRDASANVILNNLFKMTQMQTNFGFNMLAI
                     QNGIPKILSLRQILDAYIEHQKEVVVRRTRFDKEKAEARAHILEGLLIALDHIDEVIR
                     IIRASETDAEAQAELMSKFKLSERQSQAILDMRLRRLTGLERDKIQSEYDDLLALIAD
                     LADILAKPERVSQIIKDELDEVKRKFSDKRRTELMIGQVLSLEDEDLIEESDVLITLS
                     NRGYIKRLDQDEFTAQKRGGRGVQGTGVKDDDFVRELVSTSTHDHLLFFTNKGRVYRL
                     KGYEIPEYGRTAKGLPVVNLLKLDEDESIQTVINVESDRSDDAYLFFTTRHGIVKRTS
                     VKEFANIRQNGLKALNLKDEDELINVLLAEGDMDIIIGTKFGYAVRFNQSAVRGMSRI
                     ATGVKGVNLREGDTVVGASLITDQDEVLIITEKGYGKRTVATEYPTKGRGGKGMQTAK
                     ITEKNGLLAGLMTVQGDEDLMIITDTGVMIRTNLANISQTGRATMGVKVMRLDQDAQI
                     VTFTTVAVAEKEEVGTENETEGEA"
     gene            4227..>4938
                     /gene="srtA"
                     /locus_tag="VYN69_001811"
     CDS             4227..>4938
                     /gene="srtA"
                     /locus_tag="VYN69_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000078846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase SrtA"
                     /protein_id="HEV1534036.1"
                     /translation="MSQKNNKKKNKRKNLLTNILAGFLILLSLALIFNTQIRNIFIVW
                     NTNKYQVSQVSKEKLEENQDTEGNFDFDSVKAISSEAVLTSQWDAQKLPVIGGIAIPE
                     LEMNLPIFKGLDNVNLFYGAGTMKREQVMGEGNYSLASHHIFGVDNANKMLFSPLDNA
                     KNGMKIYLTDKNKVYTYEIREVKRVTPDRVDEVDDRDGVNEITLVTCEDLAATERIIV
                     KGDLKETKDYSQTSDEILT"
BASE COUNT         1534 a          848 c         1107 g         1449 t
ORIGIN      
        1 tgtagttgaa gaaaaatcct tctcagatag tctttggtgg gctcttgtga cagtaactac
       61 tgttggttat ggtgatattg ttcctgcttc catttttggg aaatggctag cagttttact
      121 gatgcttgtc ggtattggaa caatagggat gttaacaagt gctttgacga acttttttgt
      181 aaaggataat ccagatgaac agataaaact tgataaactc aaagatgaat tatcgagcca
      241 aagaatatta ctagagaaac aatctaaaaa gatagaagaa ctacataaaa tgatacaaga
      301 tttgattgaa aaaacataat aaaaataaag atgagacaaa gtttgagctg aggagaaatc
      361 aagtaagttt tgacacaata gaacgcatat tatcaggtat aaaaacttga tactatgcgt
      421 tttatagaga aataaaataa aatctgaaca aatcaattag gaatttaaag cattttataa
      481 caacgtttta gagtgatggg gtgctatttc aacttcaacc tactataata cagaaaaaaa
      541 caactccctg atgattcaag gagttgtcta tagttaaatt agtttttaga agcttcttgg
      601 aattctgggt ttttccatgc ttcgtcaatg atagcttgta attctttagc agatgcttgc
      661 attttttgag tttctgcgtc gttcaatggg atatttactg gacgaacgat accatgtgca
      721 ccaacaacag ctggttgacc gataaagaca ttctcaactc cgtattgacc ttcttggaat
      781 actgaaagtg gaagtactgc gttttcatcg tcaaggattg ctttagtgat acgagcaagg
      841 gctactgcga taccgtagta tgttgcacct tttttgttga tgattgtgta ggctgcatca
      901 cgaacacctt cgaacaattc aatcaattca gcttcttgaa cattttgagt gtctttaagg
      961 aattcttcaa ggtttacacc agcgatgtta gcgtgtgacc aaacagcgaa ctcagagtca
     1021 ccgtgttcac ccatgatgta ggcgtgcact gaacgagcat ccacatccaa tttttcagca
     1081 agtgcttgac ggaaacgagc tgagtcaagt gaagtacctg aaccgataac gcgttcttta
     1141 gggaaaccag agaatttcca agttgagtaa gtcaaaacgt caactgggtt agcagcaaca
     1201 aggaagatac ctttgaaacc agattcaaca acttgagtta cgattgattt gttgatagca
     1261 aggtttttac ctacaaggtc aagacgagtt tcacctggtt tttgaggtgc acctgcagtg
     1321 atcacaacaa ggtcagcgtc tgcacagtca gagtattgag ctgcatagat ttttttaggt
     1381 gaagtgaagg caagggcgtg actaaggtca agcgcatcac caacagcttt ttcatgcaat
     1441 tgtggaattt cgataattcc aagctcttgt gcaattcctt ggttaacaag tgcaaaagcg
     1501 taagatgaac ctacagcacc atcaccgaca aggataactt ttttgtgttg tttagttgaa
     1561 gtcattgttt taaacatctc cttaatttta ttaggggatt ttccctagac aacttcattc
     1621 tatcactttt aaaaaacttt gtcacgaata tgccttatag ttctcgatgt aaacgtttta
     1681 gtggtttaga ggctgaaata gatgggaatt tatggtataa tgttgttact tactaattgt
     1741 gaaatgaggc atttattaat gcaggataaa aatttagtga atgtcaatct gacaaaggag
     1801 atgaaggcaa gttttatcga ctacgccatg agtgttatcg tagcgcgagc tcttcctgat
     1861 gttcgagatg gcttaaaacc tgttcaccgt cgcattctct acggaatgaa tgaattgggt
     1921 gtgaccccag acaaacccca taaaaaatct gctcgtatta caggggatgt catgggtaaa
     1981 tatcacccac acggggattc ctctatttat gaagccatgg tccgtatggc tcaatggtgg
     2041 agctaccgtt acatgcttgt agatggtcat gggaattttg gttccatgga tggagatagt
     2101 gctgccgctc aacgttatac cgaggcacgt atgagcaaga ttgctctgga aatgcttcgt
     2161 gatatcaaca aaaatacagt tgatttcgtt gataactatg atgccaatga acgggaaccc
     2221 ttggtcttgc cagcgcgttt tccaaacctt ttggttaatg gagcaactgg tatcgcggtt
     2281 gggatggcaa ccaatattcc acctcataat ctgggtgaaa ccattgatgc agtgaagttg
     2341 gtcatggata atcctgaagt gactaccaag gacttgatgg aagtcttgcc tggaccagat
     2401 tttccaactg gtgctcttgt catggggaaa tcaggtatcc ataaggctta tgaaacaggt
     2461 aaaggttcga ttgtcctacg ttctcgtaca gagattgaaa cgactaagac tggtcgtgag
     2521 cgtatcgttg taacagaatt tccttacatg gtcaataaaa ccaaggtgca tgagcatatt
     2581 gttcgtttgg ttcaggaaaa acgcattgag ggtatcacag cagtacgtga tgagtcaaac
     2641 cgtgaaggtg ttcgatttgt tattgaagtc aagcgcgacg cctcagccaa tgttattctc
     2701 aataacctct tcaaaatgac ccaaatgcaa accaattttg gtttcaatat gctcgctatc
     2761 caaaatggta taccgaaaat tttgtctctt cgtcagattt tggatgctta tatcgagcac
     2821 caaaaagaag tggttgttcg tcgtacacgt tttgataagg aaaaagcgga agcgcgcgct
     2881 catatcttag aaggtctctt gattgcgcta gaccatatcg acgaagtgat tcgtatcatc
     2941 cgtgctagtg aaacggatgc ggaagctcaa gctgagttga tgagcaagtt taagctttct
     3001 gaacgtcaaa gtcaagctat ccttgatatg cgtcttcgtc gtttgacagg tttggaacgc
     3061 gataagattc aatctgagta tgatgacctc ttggctctga ttgcggattt agcagatatt
     3121 cttgctaagc ctgaacgtgt ttctcaaatt atcaaagacg aattggatga agttaaacgt
     3181 aaattttctg ataagcgccg tacagagttg atgattggac aggtcttgag tctcgaggat
     3241 gaggacttga ttgaagaatc ggatgtcttg attacccttt ctaacagagg ctacattaag
     3301 cgtttggatc aggacgagtt cactgctcaa aaacgtgggg gtcgtggtgt ccaaggaacg
     3361 ggagtgaaag atgatgactt tgttcgtgag ttagtgtcaa ctagcaccca tgatcatctg
     3421 ctcttcttca caaacaaggg acgtgtctat cgtcttaaag gttatgaaat tcctgagtat
     3481 ggtcggactg ccaaagggct accagtagtc aatctcttga aattggatga agacgaaagt
     3541 attcagacgg ttatcaatgt tgagtctgat cgcagtgatg atgcttatct cttctttaca
     3601 acccgtcacg gtattgtgaa gagaactagt gttaaggagt ttgccaatat tcgtcaaaat
     3661 ggtctcaaag cgctgaattt aaaggatgaa gatgagttaa tcaatgtctt gttggcagaa
     3721 ggagatatgg atattatcat tggtaccaag tttggttatg cagttcgctt taatcaatca
     3781 gccgttcgtg gtatgagccg tatcgccact ggtgtgaaag gtgttaacct tcgtgaagga
     3841 gacacagttg ttggtgccag cttgattact gatcaagatg aggttcttat tatcacagaa
     3901 aaaggatatg gtaagcgtac agtcgctact gaatacccaa caaaaggtcg tggtggtaag
     3961 ggaatgcaga cagctaaaat taccgaaaaa aatggcttgc tggccggtct tatgactgtt
     4021 caaggggatg aggatttgat gattatcact gatacaggtg tcatgattcg aaccaatctt
     4081 gccaatattt cacaaacagg acgtgcaact atgggagtta aagtaatgcg cctggatcaa
     4141 gatgctcaga tagtgacttt cacaacggtt gcggtggcag aaaaagaaga agttgggaca
     4201 gaaaacgaaa cagaaggtga agcataatgt ctcaaaaaaa taataaaaag aaaaacaagc
     4261 gaaaaaatct gctgacaaat atcctagcag gatttctgat attactgtca ctggctttga
     4321 tttttaatac tcaaattcga aatattttca tagtctggaa taccaataag tatcaagtta
     4381 gccaggtatc aaaagaaaaa ttagaagaaa atcaggatac agaaggcaat tttgactttg
     4441 attctgtcaa agctatctct tcggaagctg ttctaacttc tcaatgggat gctcaaaaat
     4501 taccagttat tgggggaatt gcaattcctg aattggaaat gaatttgccg atttttaaag
     4561 gacttgataa tgttaatctc ttctacggag ctggtacaat gaaacgcgag caagtaatgg
     4621 gagaaggaaa ttatagtcta gctagtcacc atatctttgg tgttgataat gctaataaaa
     4681 tgttattttc tcctttagat aatgctaaaa atggcatgaa gatttatcta accgataaaa
     4741 ataaagttta tacttatgaa atacgtgaag tcaaacgtgt gacaccagat cgtgttgatg
     4801 aagttgatga tagagatggg gtcaatgaaa tcacattagt aacctgtgaa gaccttgctg
     4861 ctacagaacg tattattgtc aaaggtgatt tgaaagaaac aaaagattat tcacaaacat
     4921 ctgatgaaat cctaacag
//
LOCUS       DAVLVP010000105         4926 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.105, whole genome shotgun sequence.
ACCESSION   DAVLVP010000105 DAVLVP010000000
VERSION     DAVLVP010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.105"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            80..268
                     /locus_tag="VYN69_001812"
     CDS             80..268
                     /locus_tag="VYN69_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HEV1534037.1"
                     /translation="MATDKNRIMISLDDKNLEKLENLVEDARDRRGMRLTKSQVIELL
                     LNTVDYFDDIMGAIYSKK"
     gene            330..1088
                     /locus_tag="VYN69_001813"
     CDS             330..1088
                     /locus_tag="VYN69_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HEV1534038.1"
                     /translation="MVVYIRQSKLPSEVSINKYHAQVGAYLQGEEAVLYQSFSEIKEL
                     TSEDIVVDYIMETRALLKMMGLNVPVHDYPIELKEFYGRKIYAGILGEIVNIPDNWGK
                     FIKPKAGSKVFTGRVVNGTHDLIGIGLPFDYPIWISEVVEFIAEWRCFVLDGRVLDVR
                     PYTGDYHAQFDASVIDEAISCWKDAPIAYGLDIGVTRDGRTLVVEVNDGYALGNYGLS
                     PLKSINFHRARWKEMVKPYFEKNEIFKIQQDVVF"
     gene            1146..2000
                     /locus_tag="VYN69_001814"
     CDS             1146..2000
                     /locus_tag="VYN69_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000900021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEV1534039.1"
                     /translation="MLGAIIGDIVGSVYEWNNIKTKDFPLFRKDCFFTDDTVMTCAVA
                     EAIMNGGQKDDFIDAMKKYGRMYPNADYGARFNAWLNSDNREPYNSFGNGSAMRISPC
                     AWIMDCGFYAKTGMWPSSRGLTSLSAEVTHNHPEGVKGAMATADAIFLCRFYFGGYCR
                     EYEQSINDNPTECKRRIKDYIEKEYDYNLSQTLDEIRPNYRFNETCQETVPQAIIAFL
                     ESRDFEDAIRNAISLGGDSDTLAAITCSIAEATYGIPDWIKDKAYSYLDEPLKDVVRR
                     WENRIKAY"
     gene            2112..2996
                     /locus_tag="VYN69_001815"
     CDS             2112..2996
                     /locus_tag="VYN69_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000710121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEV1534040.1"
                     /translation="MKKAMVIINPTSGGEKALDYKEKLENKAKEYFEYVETKITEKAL
                     DATHFAEEASREQYDAVVVFGGDGTVNEVISGIDERDYIPKLGIIPGGTGNLITKLLE
                     INQDIDGAIDELDFDLTNKIDIGKANDNYFGYIFSIGSLPEAIHNVEIEDKTKFGILT
                     YAVNTMKSVMTDQVFNIKVETENGNYVGEASHVLVLLTNYFADKKIFEENKDGYANIL
                     ILKDASIFSKLSVIPDLLKGDVIANDNIEYIKARNIKISSDSELESDVDGDKSDNLPV
                     EIKVLAQRVEVFSKPKED"
     gene            3159..3932
                     /locus_tag="VYN69_001816"
     CDS             3159..3932
                     /locus_tag="VYN69_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000423448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha amylase N-terminal ig-like
                     domain-containing protein"
                     /protein_id="HEV1534041.1"
                     /translation="MELSAIYHGPESEYAYLYKDKKLHIRIRTKKGDIESINLHYGDP
                     FIFMEEFYQDTKEMVKITSGTLFDHWQVEVSVDFARIQYLFELRDTEGQNILYGDKGC
                     VENSLENLHAIGNGFKLPYLHEIDACKVPDGVSNTVWYQIFPERFANGNALLNPEGTL
                     DWDSSVTPKSDDFFGGDLQGIIDHMDYLQDLGITGLYLCPIFESTSNHKYNTTDYFEI
                     DRHFGDKETFRELVDQAHHRGMKVMLDAVFNHIGSQSLQ"
     gene            3999..4898
                     /locus_tag="VYN69_001817"
     CDS             3999..4898
                     /locus_tag="VYN69_001817"
                     /inference="COORDINATES: protein motif:HMM:NF012356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HEV1534042.1"
                     /translation="MTTEKLVNKRDLPYHVFGFEDYMPKLNTANPEVKNYLLKVATYW
                     IEEFNIDAWRLDVANEIDHQFWKDFRKAVLAKNPDLYILGEVWHTSQPWLNGDEFHAV
                     MNYPLSDSIKDYFLRGIKNTDQFIDEINGESMYYKQQISEVMFNLLDSHDTERILWTA
                     NEDVQLVKSALAFLFLQKGTPCIYYGTELALTGGPDPDCRRCMPWERVSSDNDMLNFM
                     KRLIKIRKYASVIISHGKYSLQEIKSDLVALEWKYEGRILKAIFNQSTEDYLLEKEAV
                     VLASNCQELENQLVISPDGFVIF"
BASE COUNT         1613 a          759 c         1053 g         1501 t
ORIGIN      
        1 cacttttaaa ctaatgtatt ataaaagcca gttgacacaa gtgttacacc tgtgatagaa
       61 ttaacttgag gtgatgaaaa tggcaaccga taaaaataga ataatgatta gcttggatga
      121 taaaaatcta gagaaacttg aaaatttagt agaagatgct agagatagaa ggggaatgcg
      181 actaacaaag tcccaggtta ttgaattact cctaaatacc gttgattatt tcgatgatat
      241 tatgggggct atttactcaa aaaaataaga gcaaaattgc tattattttt tacaaattgg
      301 tgtaacacta gtgatatagg agtgatgaaa tggttgttta tattagacaa agtaaattac
      361 caagtgaggt ttcaattaat aaataccatg ctcaagtagg tgcctactta caaggagaag
      421 aagctgtttt atatcaatct ttttctgaaa taaaagagtt aacaagtgaa gatatagttg
      481 tagattatat aatggagact agagcattac taaaaatgat gggcttaaac gttccggttc
      541 atgactatcc tattgagctt aaagagtttt atggtcgaaa gatttatgct ggtattttag
      601 gagagattgt gaatatacct gataattggg gaaaatttat taagcctaaa gctggttcaa
      661 aagtctttac tggaagagtt gttaatggaa cccatgattt aataggtatt ggtctacctt
      721 tcgactatcc tatatggatt agtgaggttg tagaattcat agctgaatgg cgttgttttg
      781 tgttagatgg tcgcgtatta gatgttcgac cctatacagg tgattatcat gcacaatttg
      841 atgcaagtgt aattgatgaa gcgatatcat gttggaaaga tgcgccaata gcttatggac
      901 tagatatcgg tgttactcgc gatggcagaa cacttgttgt tgaagtaaat gatggttatg
      961 cattgggaaa ttatggccta tctcctttaa aatcaatcaa ttttcataga gctaggtgga
     1021 aagaaatggt aaaaccctat tttgaaaaaa atgaaatttt taaaatccaa caggatgttg
     1081 ttttctaaaa taaatatggt agataaccaa tataaaaagc atcaaaatga acaaggagga
     1141 atctgatgct tggagcaatt attggagata ttgtcggttc tgtttacgaa tggaacaata
     1201 tcaaaacaaa ggactttcct ttatttcgga aggactgctt ttttacagat gacacggtta
     1261 tgacctgtgc tgttgcagaa gcaattatga atggtggaca aaaagacgat ttcattgacg
     1321 cgatgaagaa atatggcaga atgtatccga atgctgatta cggtgctcgg tttaatgcat
     1381 ggctaaacag cgataaccgt gagccttata atagctttgg gaatggatca gctatgcgta
     1441 tttctccatg tgcttggatc atggactgtg gtttttatgc gaaaactggt atgtggccat
     1501 catctagagg acttacgagt ctttctgcag aggtaactca taatcatcca gaaggtgtca
     1561 aaggcgctat ggctacagct gatgctatct ttctatgtcg tttttacttt ggtggttatt
     1621 gtagagagta cgagcaatca attaacgaca atcctacaga gtgtaaaaga cgaattaagg
     1681 attatataga aaaagaatac gattacaatc tatctcaaac tttagatgaa atccgtccta
     1741 actatcgttt taacgaaaca tgtcaggaaa ctgtccctca agccattatc gcctttcttg
     1801 aaagtagaga cttcgaagat gcgataagaa atgccatctc acttggtggc gacagtgata
     1861 ctctcgcagc aatcacttgt agcatagcag aggcaactta cggtattcct gattggatca
     1921 aggataaggc ctattcttac ttggatgaac ccttaaagga tgtagttagg cgatgggaaa
     1981 atagaataaa agcgtattaa tctccagtac gtcagatttc tcttttctgt attttcattg
     2041 tataatagga ttacatttac ttgcatacga cgaaatgtaa acatttctgt ttaaaattaa
     2101 ggataaaatc aatgaaaaaa gcaatggtaa ttatcaaccc tacttctggt ggcgagaagg
     2161 ctttggatta caaagaaaag ctggagaata aagcaaaaga atactttgaa tatgttgaaa
     2221 ccaaaattac cgaaaaagcg ctggatgcaa cacattttgc tgaagaagct tctcgtgagc
     2281 agtatgatgc agtggttgtg tttggtggag atggaactgt caatgaagtc atttcaggta
     2341 ttgatgagag agactacatt cctaagttag ggattatccc aggcggtacg ggtaacctca
     2401 ttacaaaact tttggaaatc aatcaagaca tcgatggcgc aattgacgaa ctggattttg
     2461 atttaaccaa taagattgat atcggtaaag caaatgacaa ctattttggt tatatcttta
     2521 gtatcggttc tctgcctgag gcgattcaca atgttgaaat agaggacaaa acaaaattcg
     2581 gtattttaac ctatgctgta aataccatga agtctgtcat gacagatcag gtctttaaca
     2641 ttaaggttga gacagaaaat ggaaattatg ttggtgaagc tagccatgtt ttggtccttt
     2701 tgacaaatta cttcgctgat aagaaaatct ttgaagaaaa caaggacggc tatgccaaca
     2761 ttttgattct gaaagatgcc tctatattct ccaaattatc cgtcattcct gatttattaa
     2821 aaggggatgt tatcgcaaat gataatatcg agtatatcaa agcgcgtaat attaaaatct
     2881 cttcagatag tgaattggag tcagatgttg acggagataa atcagataac ctacctgtag
     2941 aaatcaaagt cctagctcag cgagtagaag tattttcaaa accgaaagag gattagtata
     3001 tagagaaagc cttttttaag gctttttgta tactttaaaa gatagttcct ttaacaacgg
     3061 acattccttg caaatagttt acaaaaatag tatactggat ttattgagtt tgaaaacgtt
     3121 tgcgtaaaat ttgaatgaat actttaggag acaaattgat ggaattaagt gctatttacc
     3181 atgggcctga gtcggagtat gcctatcttt ataaggataa gaaactccat attcgaattc
     3241 gaactaagaa aggggacatt gaaagcatca acttgcacta tggggaccct tttatcttta
     3301 tggaggagtt ttatcaggat acaaaagaaa tggtcaagat aacttctggt accttatttg
     3361 accattggca ggttgaagtg tcagttgact ttgcacgtat ccagtatctc tttgagctca
     3421 gagatacaga aggtcaaaat attttgtatg gcgataaagg gtgtgtggaa aattctctag
     3481 aaaatcttca tgcaattggg aatggattta agttgcctta tcttcatgag attgatgcct
     3541 gcaaggttcc tgacggggtt tcaaatacgg tatggtatca gatatttcct gaaagatttg
     3601 ccaatggcaa tgctctatta aacccagaag ggactttaga ctgggattca tctgtcacac
     3661 ctaagagtga tgatttcttt ggtggtgatt tacaggggat tattgatcat atggattact
     3721 tgcaagactt gggtattact ggactatatc tttgtcccat ctttgaatct acaagcaatc
     3781 acaagtacaa tacgacagat tactttgaaa ttgaccgtca ttttggagac aaggagacct
     3841 ttcgggaact ggtggatcaa gcgcatcatc gtggcatgaa agtcatgctg gatgcggtat
     3901 ttaatcatat tggttcgcaa tctcttcaat gaaaaaatgt cgtcaaaaat ggtgaacagt
     3961 ctgcttataa ggattggttc catattcaac aattcccagt gacaactgaa aagctagtta
     4021 ataagagaga cttaccctat catgtttttg gtttcgagga ctatatgcct aagctaaata
     4081 cagccaatcc agaggtcaag aattatcttt taaaggttgc gacttattgg attgaagagt
     4141 ttaatatcga tgcttggcgt ttggatgtgg ctaatgagat tgaccatcag ttctggaagg
     4201 attttcgtaa ggcagtttta gctaaaaatc ctgatcttta tatcctagga gaagtctggc
     4261 atacatctca gccttggcta aatggagatg agttccatgc cgtcatgaat tatcctttat
     4321 ctgatagtat caaggactat ttcttacgag gaattaagaa tacagaccag ttcatcgatg
     4381 aaatcaatgg agagtctatg tattacaagc agcagatttc agaggtcatg tttaatctct
     4441 tggattcaca tgatacagag cgaatcctgt ggacggccaa tgaagatgtt caactggtta
     4501 aatcagcctt agcctttctc tttttacaaa aaggaacacc gtgcatttat tacggaaccg
     4561 agctagcctt gactggagga ccagatccag attgtcgtcg ttgtatgcct tgggaacgtg
     4621 tatcaagtga caatgatatg ctgaacttta tgaagaggct gattaagatt cggaaatacg
     4681 cgtcagtaat catttcgcat ggcaagtata gccttcaaga aatcaaatct gatctagtag
     4741 ctctggaatg gaaatacgaa ggacggatcc tcaaagcaat attcaaccaa tcaacagaag
     4801 attatctttt agagaaagaa gcagtagtac tagcaagcaa ttgccaagaa ttggagaatc
     4861 agcttgtcat ctctccagat ggatttgtga ttttctaaaa actagttgat gaagattatg
     4921 gtacat
//
LOCUS       DAVLVP010000106         4889 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.106, whole genome shotgun sequence.
ACCESSION   DAVLVP010000106 DAVLVP010000000
VERSION     DAVLVP010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4889)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4889)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4889
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.106"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            75..656
                     /locus_tag="VYN69_001818"
                     /pseudo
     CDS             75..656
                     /locus_tag="VYN69_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018164186.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
     gene            813..1181
                     /locus_tag="VYN69_001819"
     CDS             813..1181
                     /locus_tag="VYN69_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1534043.1"
                     /translation="MAVGLSYEDPAVKLLNDGANIKVVYPKEGTVFLPASAAIVKKAK
                     NMENAKKFIDFIISQEVQDTLGTTTTNRPVRKNAKTSENMKPIDKIKTLTEDYDYVIK
                     NKSDIVKKYNEVFTDIQSKQ"
     gene            1194..1580
                     /locus_tag="VYN69_001820"
     CDS             1194..1580
                     /locus_tag="VYN69_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001289359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1534044.1"
                     /translation="MSEIEIINAKKIYHDVPVIENLNITIPKGSLFTLLGASGCGKTT
                     LLRMIAGFNSIKDGEFYFDDTKINNMEPSKRNIGLVFQNYAIFPHLTVRDNVAFGLMQ
                     KKVPKEELIQQTNKYLELMQIAQYAD"
     gene            1587..1676
                     /locus_tag="VYN69_001821"
                     /pseudo
     CDS             1587..1676
                     /locus_tag="VYN69_001821"
                     /inference="COORDINATES: protein motif:HMM:NF012235.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            1662..2285
                     /locus_tag="VYN69_001822"
     CDS             1662..2285
                     /locus_tag="VYN69_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001843667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1534045.1"
                     /translation="MDEPLSNLEAKLRLDMRQAIRAIQHEVGITTVYVTHDQEEAMAI
                     SDQIAVMKDGVIQQIGRPKELYHKPANEFVATFIGRTNIIPTNLEKRSDGAYIVFSDG
                     YALRMPALDQVEEQAIHVSIRPEEFIKDESGDIEGTISDSVYLGLNTEYFIETGFASK
                     IQVSEESTFEEELQKGNRIRLRINTQKLNIFSADGSQNLIKGVNHGM"
     gene            2275..3920
                     /locus_tag="VYN69_001823"
                     /pseudo
     CDS             2275..3920
                     /locus_tag="VYN69_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520796.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
     gene            complement(4023..4715)
                     /locus_tag="VYN69_001824"
     CDS             complement(4023..4715)
                     /locus_tag="VYN69_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEV1534046.1"
                     /translation="MVKVRLYLIRHGKTMFNTIGRAQGWSDTPLTAEGERGIQELGIG
                     LRESDLQFERAYSSDSGRTIQTMGIILEELGLQGKIPYRMDKRIREWCFGSFDGAYDG
                     DLFMGIIPRIFNVDHVHQLSYAELAEGLVEVDTAGWAEGWEKLSGRIKEGFEMIAKEM
                     EDQGGGNALVVSHGMTIGTIVYLINGMHPHGLDNGSVTILEYEDGQFRVEVVGDRSYR
                     ELGREKMEEGSI"
BASE COUNT         1533 a         1045 c          843 g         1468 t
ORIGIN      
        1 tttaaaaacg cttacattgt tttaaaataa acttaaataa cacaacggag atatccatca
       61 tgaaaaagaa atagatgtat tatgctgctt gttcttctaa tgaatctgcc gatgacagtt
      121 catctgataa aggagacggt ggttcgctag tcgtttattc accaaactca gaaggcttaa
      181 ttggagcaac tattcctgcc tttgaagaaa aatatggtat caaaatagaa ctgattcaag
      241 ctggtactgg agaacttttc aaaaaactag agtcagaaaa agaagttcct gtagctgatg
      301 ttatctttgg tggttcttat acacaatatg ctacccacgg agaactcttt gaaaactata
      361 tttcaaaaga aaatgataat gttatcaaag aatatcaaaa cacaactggc ttctctactc
      421 cttatacact agatggtagt gttttaatcg tccaccctga tttaactaaa ggcatgaaca
      481 tcgaaggata tagcgacctt ctcaaacctg aactaaaagg aaaaatcgca actgctgacc
      541 cagcaaactc ttctagcgcc tttgctcaat taacaaatat gctacaagct caaggtggtt
      601 acaaagatga taaggcttgg tcttatgtaa aagatctttt cacacttatt gatggtatag
      661 taaaatgaaa taagaacagg acaaattaat caggacagtc aaatcgattt ctaacaatgt
      721 tttagaagta gaggtgtact attctagttt caatacacta taaaatcggt tcaagttcat
      781 ctggtgtcta taaagcagtc gttgatggag aaatggctgt tggtctctct tatgaggatc
      841 cagcagttaa actcttaaat gacggagcta acattaaagt agtctatcca aaagaaggaa
      901 ccgtcttcct acctgctagt gctgctatcg ttaaaaaagc taaaaatatg gaaaatgcca
      961 agaaatttat cgattttatt atctctcaag aagtacaaga tacacttggc acaaccacta
     1021 ctaaccgtcc tgttcgtaaa aatgctaaaa caagcgaaaa catgaaacca attgacaaaa
     1081 tcaaaacact cactgaagat tatgattatg tcatcaagaa taaatcagat atcgttaaga
     1141 aatacaacga agtctttaca gatatccaat ctaaacagta aaagaggttc actatgagtg
     1201 agatcgaaat tattaacgcc aaaaaaatct accacgatgt ccctgttatt gagaatttga
     1261 acattacaat tccaaaagga agtctcttta cccttcttgg agcttcagga tgtgggaaaa
     1321 cgacccttct tcgtatgatt gcaggtttca acagtatcaa agatggagaa ttttacttcg
     1381 atgatacaaa aatcaataat atggaaccca gcaaacgcaa tatcgggctg gttttccaaa
     1441 actacgctat tttcccacat ttgactgtcc gagacaacgt tgcttttggt cttatgcaaa
     1501 agaaggttcc aaaagaagaa ttgattcaac agaccaacaa gtatcttgaa ctcatgcaaa
     1561 ttgctcaata tgcggattga aagcccgata aactcagtgg tggacaacaa caacgtgtca
     1621 ccttggcatg cgccttagcg gttaatccaa gtgttctcct catggacgag ccacttagta
     1681 atctggaggc caaacttcgc ttggatatgc gtcaagccat ccgagcaatc caacacgaag
     1741 tgggaattac aactgtttat gtaacccacg accaagaaga agccatggct atttcagacc
     1801 aaattgctgt tatgaaagat ggggtgatcc aacaaatcgg ccgaccaaaa gaactctatc
     1861 ataaaccagc taatgagttt gtggcaacct ttatcggacg cacaaatatt atccctacta
     1921 atcttgaaaa acggagcgac ggcgcttata tcgtcttttc agatggctat gcccttcgaa
     1981 tgccagctct tgatcaggtt gaggagcaag ctattcatgt aagcattcgt cccgaagagt
     2041 ttatcaaaga tgaatctgga gatattgaag gaactattag cgatagcgtc tatcttggac
     2101 taaatacgga gtatttcatt gagacaggtt ttgcctcaaa aattcaagtt agtgaagaat
     2161 caacttttga agaagaacta caaaaaggca atcgtattcg tctacgaatc aatacgcaaa
     2221 aattaaacat cttttctgca gatggttccc aaaacctgat aaaaggagtc aaccatggaa
     2281 tgtaaaaaac taaatatttg gacaacctcc tctttcttca tctttcttac ctatcttgtc
     2341 tttctcgttt atcctatcgt taccgtgctc aagcaagcac ttatacatga aggacaattc
     2401 tcactagcta attttgtcac tttctttagt aaagtctact actctgagac actagtcaac
     2461 agtttcaagg tttccattac cgctactgtc acttccttag ttgtaggaac cctattagct
     2521 tatctcttct ctatgtatga cttcaagggg aagaaatttc tacaaatatt gattatcatt
     2581 gcttccatgt cagctccttt cgtaggagcc tactcctggg ttctcttgct aggacgaaat
     2641 ggggtcatca ctaaattttt gacaaatgcc cttcatcttc cagctatcga tatttatgga
     2701 ttcaaaggaa ttgtacttgt ctttacactg caactattcc cactggtatt tctatatgtt
     2761 gctggaacaa tgaacagtat tgacaattct ctacttgaag cagctgaaag catggggtcc
     2821 ttcggattta aacctatcgt aacggttgtt ttacctctcc tagttccaac cttactagca
     2881 gctccttgct tgtatttatg agagcattct cagactttgg aacgcctatg ttgattggcg
     2941 aaggatatcg gactttccct gtcctgattt atacccaatt tattagcgag gttggaggaa
     3001 attctgcttt tgcaattatg gcgattatca ttgccttggc aattttcctt atccaaaaac
     3061 acattgcaaa ccgctacagt ttcagcatga atctgctcca tccaattgag cctaaaaaaa
     3121 ctacaaaagg aaaaatggct gccatttatg caacagtcta cggaattatc tttatctctg
     3181 ttttacctca aatctactta atttatacct ctttcctaaa aacatcaggt atggtatttg
     3241 ttaaaggtta ttctccaaac agttacaagc tagctttcaa tcgtatggga tctgctattt
     3301 tcaataccat tcgtatccct ttgattgcct tagttctagt tgttctattt gcgacattta
     3361 tctcctacct agccgttaga aaacggaatt tgtttacaaa cttaattgac agcctcagta
     3421 tggtacctta tattgtacca ggaaccgttc tagggattgc cttcatttct tccttcaata
     3481 ctggtctatt tggaagtgga tttcttatga ttacagggac tgctttcatc ttgattatgt
     3541 ctctatctgt cagaagatta ccgtatacta ttcgctcatc tgttgctagc ttacaacaaa
     3601 tagcaccaag tattgaagaa gctgctgaaa gcttaggaag tagtcgtctc aatatctttg
     3661 ctaagattac aactccaatg atgctatcgg gtatcatttc tggagccatc ttatcttggg
     3721 tcacaatgat ttcaaaactc tctacttcta tcctcctcta caatgtcaaa acaagaacaa
     3781 tgactgtagc tatttataca gaggttctca gaggaaatta cggtgtagcc gcagccttgt
     3841 caactatcct gactgttcta acagtaggtt ccttgctctt gtttatgaaa atctctaaaa
     3901 gcaatagcat tacactttag ttttccacat caaaaacagc cgaaaggagt accctcagct
     3961 gttttttctt atcttgatat tcttatcaaa tccctagctc atggcaagca agtctagact
     4021 gattaaatag agccttcttc catcttctca cgtcctagct ctcggtaact acggtcaccg
     4081 acaacttcaa ccctaaactg accgtcctca tattcaagaa tcgtcacgct accattatct
     4141 agaccatgcg gatgcatgcc attaatcaga taaacaatgg ttccaatagt cattccatgg
     4201 ctgacaacaa gggcgttacc tccaccttga tcttccattt cttttgcaat catttcaaag
     4261 ccttccttaa ttcggccact gagtttttcc cagccttcag cccaaccagc tgtatcgacc
     4321 tctaccaagc cctcagccag ttcagcataa gacaattggt gaacgtggtc cacattaaag
     4381 atacgaggaa taatgcccat gaaaagatcg ccatcatagg ctccatcaaa actaccgaaa
     4441 caccattctc tgatacgctt gtccatgcga taagggattt tcccctgcaa gccaagctct
     4501 tcaaggataa ttcccatggt ctgaatggta cgaccagaat cactcgaata agcgcgctca
     4561 aactgtagat cagattctcg caaaccgatt cctaactctt gaatccctcg ttcaccttca
     4621 gcagttaagg gagtatcgct ccaaccttgc gcgcgaccaa tcgtgttaaa catggtcttg
     4681 ccatgacgta tcaaatacaa tcgtactttt accattttcc gtcctccgtt tgcttctatt
     4741 atatcatgaa tgataaaata aaagccaccc atacaggcga cttttgaagg agattattat
     4801 gaaaaagttt aggagttcat taaataaaga tattagatga aaatcaaatt caaactaatt
     4861 cagtgttatc tgtttcaaat aatattagg
//
LOCUS       DAVLVP010000107         4875 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.107, whole genome shotgun sequence.
ACCESSION   DAVLVP010000107 DAVLVP010000000
VERSION     DAVLVP010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4875
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.107"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(76..216)
                     /gene="comC"
                     /locus_tag="VYN69_001825"
     CDS             complement(76..216)
                     /gene="comC"
                     /locus_tag="VYN69_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004238992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence-stimulating peptide ComC"
                     /protein_id="HEV1534047.1"
                     /translation="MKNTVKLEQFVALKEKDLQNIQGGEMRKMNEKSFNIFNFFNFFR
                     RR"
     gene            complement(381..454)
                     /locus_tag="VYN69_001826"
     tRNA            complement(381..454)
                     /locus_tag="VYN69_001826"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(418..420),aa:Arg,seq:tct)
     gene            complement(498..977)
                     /gene="rlmH"
                     /locus_tag="VYN69_001827"
     CDS             complement(498..977)
                     /gene="rlmH"
                     /locus_tag="VYN69_001827"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEV1534048.1"
                     /translation="MKIKVVTVGKLKEKYLKDGIAEYSKRISRFAKFEMIELSDEKTP
                     DKASESENQKILEIEGQRILSKIADRDFVIVLAIEGKTFFSEEFSKQLEETSIKGFST
                     LTFIIGGSLGLSSSVKNRANLSVSFGRLTLPHQLMRLVLVEQIYRAFTIQQGFPYHK"
     gene            1160..2341
                     /locus_tag="VYN69_001828"
     CDS             1160..2341
                     /locus_tag="VYN69_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HEV1534049.1"
                     /translation="MKHLKTFYKKWFQLLVVIVISFFSGALGSFSITQLTQKSSVNNS
                     NNNSTITQTAYKNENSTTQAVNKVKDAVVSVITYSANRQNSVFGNDDTDTDSQRISSE
                     GSGVIYKKNDKEAYIVTNNHVINGASKVDIRLSDGTKVPGEIVGADTFSDIAVVKISS
                     EKVTTVAEFGDSSKLTVGETAIAIGSPLGSEYANTVTQGIVSSLNRNVSLKSEDGQAI
                     STKAIQTDTAINPGNSGGPLINIQGQVIGITSSKIATNGGTSVEGLGFAIPANDAINI
                     IEQLEKNGKVTRPALGIQMVNLSNVSTSDIRRLNIPSNVTSGVVVRSVQSNMPANGHL
                     EKYDVITKVDDKEIASSTDLQSALYNHSIGDTIKITYYRNGKEETTSIKLNKSSGDLE
                     S"
     gene            2399..3157
                     /locus_tag="VYN69_001829"
     CDS             2399..3157
                     /locus_tag="VYN69_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEV1534050.1"
                     /translation="MEKFEMISITDIQKNPYQPRKEFDREKLDELAQSIKENGVIQPI
                     IVRQSPVIGYEILAGERRYRASLLAGLRSIPAVVKQISDQEMMVQSIIENLQRENLNP
                     IEEARAYESLVEKGFTHAEIADKMGKSRPYISNSIRLLSLPEQILSEVENGKLSQAHA
                     RSLVGLNKEQQDYFFQRIIEEDISVRKLEALLTEKKQKKQQKTNYFIQNEEKQLRKLL
                     GLDVEIKLSKKDSGKIIIYFSNQEEYSRIINSLK"
     gene            3370..4731
                     /gene="dnaA"
                     /locus_tag="VYN69_001830"
     CDS             3370..4731
                     /gene="dnaA"
                     /locus_tag="VYN69_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004255267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEV1534051.1"
                     /translation="MKEKQFWNRILEFAQERLTRSMYDFYAIQAELIKVEENVATIFL
                     PRSEMEMVWEKQLKDIIVVAGFEIYDAEITPHYIFTKPQDTTSSQVEEATNLTLYDYS
                     PKLVSIPYSDTGLKEKYTFDNFIQGDGNVWAVSAALAVSEDLALTYNPLFIYGGPGLG
                     KTHLLNAIGNEILKNIPNARVKYIPAESFINDFLDHLRLGEMEKFKKTYRSLDLLLID
                     DIQSLSGKKVATQEEFFNTFNALHDKQKQIVLTSDRSPKHLEGLEERLVTRFSWGLTQ
                     TITPPDFETRIAILQSKTEHLGYNFQSDTLEYLAGQFDSNVRDLEGAINDITLIARVK
                     KIKDITIDIAAEAIRARKQDVSQMLVIPIDKIQTEVGNFYGVSIKEMKGSRRLQNIVL
                     ARQVAMYLSRELTDNSLPKIGKEFGGKDHTTVIHAHAKIKSLIDQDDNLRLEIESIKK
                     KIK"
BASE COUNT         1714 a          863 c          802 g         1496 t
ORIGIN      
        1 acggtaacta tgactaataa ttaaaaaata taatatcaaa tctactaata agaaaaaatc
       61 cattctgcta ctcctttatc ttcttctaaa gaaattaaag aaattaaaga tattaaagga
      121 cttttcattc attttcctca tctccccacc ttgaatattt tgcaagtctt tttccttcaa
      181 agctacaaac tgttccaatt taactgtgtt tttcataata aaatctccta aaatgttttt
      241 tcttgtaagc taacttacaa aaaccattat acaaaatgga atttcgtttt agataaaatt
      301 ctctcaactg tcattttttt ctcccaaagt gtactttttt aagaaaaaag ccgggaaaat
      361 tcccagcttt gctattatat tgatcccagc aggattcgaa cctgcgaccg ttcgcttaga
      421 aggcgaatgc tctatccagc tgagctatga gacctaacac aattattcta ccaaaaattc
      481 aattaaaagt caattttcta tttatggtag gggaatccct gctgaatcgt aaaagcgcga
      541 tagatttgtt caacaagaac tagtctcatt aactgatggg gtaaggttag gcgaccaaaa
      601 ctgacagaaa gattggctct attttttaca gatgatgaca atcctaaact tcccccaata
      661 ataaaagtaa gagtagaaaa tccttttata gaagtttctt ctaactgctt actaaattct
      721 tctgagaaga aagttttccc ttcaatggct aacacaataa cgaaatcacg gtcagcaatt
      781 tttgataaaa ttctctgacc ttctatttct aaaatctttt gattttctga ttcactggcc
      841 ttatctggtg ttttttcatc tgataactca atcatttcaa acttagcaaa tctagaaatt
      901 cgttttgaat actctgcgat accatctttt aaatactttt ctttcagttt cccaactgtt
      961 acaactttaa ttttcatgac tctattctaa catattctct attttttcac atcttattca
     1021 caaaataaaa aatagatttc aattaagaaa atcacagttt caaaagagtt atccacagtt
     1081 tgtgtaaaac ttttgtgttt aagttataat taagctagtc agtttatact ttcagtaatt
     1141 caaacatatg gaggcaaata tgaaacatct aaaaacattt tacaaaaaat ggtttcaatt
     1201 attagtcgtt atcgtcatta gcttttttag tggagccttg ggtagttttt caataactca
     1261 actaactcaa aaaagtagtg taaacaactc taacaacaat agtactatta cacaaactgc
     1321 ctataaaaac gaaaattcaa caacacaggc tgttaacaaa gtaaaagatg ctgttgtttc
     1381 tgttattact tattcggcaa acagacaaaa tagcgtattt ggcaatgatg atactgacac
     1441 agattctcag cgaatctcta gtgaaggatc tggagttatt tataaaaaga atgataaaga
     1501 agcttacatc gtcaccaaca atcacgttat aaatggcgcc agcaaagtag atattcgatt
     1561 gtcagatggg actaaagtac ctggagaaat tgtcggagct gacactttct ctgatattgc
     1621 tgtcgtcaaa atctcttcag aaaaagtgac aacagtagct gagtttggtg attctagtaa
     1681 gttaactgta ggagaaactg ctattgccat cggtagcccg ttaggttctg aatatgcaaa
     1741 tactgtcact caaggtatcg tatccagtct caatagaaat gtatccttaa aatcggaaga
     1801 tggacaagct atttctacaa aagccatcca aactgatact gctattaacc caggtaactc
     1861 tggcggccca ctgatcaata ttcaagggca ggttatcgga attacctcaa gtaaaattgc
     1921 tacaaatgga ggaacatctg tagaaggtct tggtttcgca attcctgcaa atgatgctat
     1981 caatattatt gaacagttag aaaaaaacgg aaaagtgacg cgtccagctt tgggaatcca
     2041 gatggttaat ttatctaatg tgagtacaag cgacatcaga agactcaata ttccaagtaa
     2101 tgttacatct ggtgtagttg ttcgttcggt acaaagtaat atgcctgcca atggtcacct
     2161 tgaaaaatac gatgtaatta caaaagtaga tgacaaagag attgcttcat caacagactt
     2221 acaaagtgct ctttacaacc attctatcgg agacaccatt aagataacct actatcgtaa
     2281 cgggaaagaa gaaactacct ctatcaaact taacaagagt tcaggtgatt tagaatctta
     2341 attgacatct atgtaaagaa agctttacat aagagaaaag atgtgttagt gtagaatcat
     2401 ggaaaaattt gaaatgattt ctatcacaga tatacaaaaa aatccctatc aaccccgaaa
     2461 agaatttgat agagaaaaac tagatgaact agcacagtct atcaaagaaa atggggtcat
     2521 tcaaccgatt attgttcgtc aatctcctgt tattggttat gaaatccttg caggagagag
     2581 acgctatcgg gcttcacttt tagctggtct acggtctatc ccagctgttg ttaaacagat
     2641 ttcagaccaa gagatgatgg tccagtccat tattgaaaat ttacagagag aaaatttaaa
     2701 cccaatagaa gaagcacgcg cctatgaatc tctcgtagag aaaggattca cccatgctga
     2761 aattgcagat aagatgggca agtctcgtcc atatatcagc aactccattc gtttactttc
     2821 cttgccagaa cagattctct cagaagtaga aaatggcaaa ctatcacaag cccatgcgcg
     2881 ttccctagtt gggttaaata aggaacaaca agactatttc tttcaacgga ttatagaaga
     2941 agatatttct gtaaggaagt tagaagctct tctgacagag aaaaaacaaa agaaacagca
     3001 aaaaactaat tatttcatac aaaatgaaga aaaacagtta agaaaactac tcggattaga
     3061 tgtagaaatt aaactatcta aaaaagacag tggaaaaatc attatttatt tttcaaatca
     3121 agaagaatat agtagaatta tcaacagcct gaaataaggc tgttctttta tttttttatc
     3181 tcacaaggtt atccactatg tttttcgata aaaagcttaa taaatcaata atttcttctt
     3241 ttatccccaa cctgtggata aagtttagta acattgtgga ttatttttca cagcttgtgg
     3301 aaaattcttg ctatctatgg taaaatatct ctagtattaa acttttaaat agtaaaggag
     3361 gagaaaggat tgaaagaaaa acaattttgg aatcgtatat tagaatttgc acaagaaaga
     3421 ctgactcgct ccatgtatga tttctatgct attcaagctg aactcatcaa ggtagaggaa
     3481 aatgttgcca ctatatttct acctcgctct gaaatggaaa tggtctggga aaaacaacta
     3541 aaagatatta ttgtagtagc tggttttgaa atttatgacg ctgaaataac tccccactat
     3601 attttcacca aacctcaaga tacgactagc tcacaagttg aagaagctac aaatttaact
     3661 ctttatgact atagtccaaa gttagtatct attccttatt cagatacggg attaaaagaa
     3721 aagtatacct ttgataactt tattcaaggg gatggaaatg tttgggctgt gtcagccgct
     3781 ttagctgtct ctgaagattt ggctctgacc tataaccctc tttttatcta tggaggacca
     3841 ggccttggta agactcactt attaaacgct attggaaatg aaattctaaa aaatattcct
     3901 aatgcgcgtg ttaaatatat ccctgccgaa agctttatta atgactttct tgatcaccta
     3961 agacttgggg aaatggaaaa gtttaaaaag acctatcgta gtcttgatct tttgttaatc
     4021 gatgatatcc agtcactcag cggaaaaaaa gtcgcaactc aggaagaatt tttcaatacc
     4081 tttaacgccc ttcatgacaa gcaaaaacag attgtcctaa cgagtgatcg tagtccaaaa
     4141 catctagaag ggctcgagga gaggcttgtc acgcgtttta gttggggatt gacacaaact
     4201 atcacacccc ctgactttga aacacgtatt gccattttac aaagtaaaac ggaacattta
     4261 ggctacaatt tccaaagtga tactctagaa tacctagctg ggcaatttga ttcaaatgtt
     4321 cgagatcttg agggagccat caacgacatc actttaattg ccagagtaaa aaaaatcaag
     4381 gatatcacta ttgatattgc tgcagaagcc attagagccc gcaaacaaga tgttagccaa
     4441 atgctcgtca tcccaattga taaaatccaa actgaagttg gtaactttta tggtgttagt
     4501 atcaaagaaa tgaagggaag tagacgcctt caaaatattg ttttggcccg tcaagtagcc
     4561 atgtatttat ctagagaact aacagataat agtcttccaa aaattgggaa ggaatttggg
     4621 ggaaaagatc ataccacagt cattcatgcc catgccaaaa taaaatcttt gattgatcaa
     4681 gacgataatt tacgtttaga aattgaatca atcaaaaaga aaatcaaata atttgtggat
     4741 aacttttagt tttttatctt ttttatccac attttttaaa caagctaaaa aacttgatat
     4801 gacttgttta aaggctgttt tccacagatt tcacagactc tattattact attatctttc
     4861 taatactaaa aataa
//
LOCUS       DAVLVP010000108         4843 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.108, whole genome shotgun sequence.
ACCESSION   DAVLVP010000108 DAVLVP010000000
VERSION     DAVLVP010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4843)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4843)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4843
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.108"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            382..1577
                     /locus_tag="VYN69_001831"
                     /pseudo
     CDS             382..1577
                     /locus_tag="VYN69_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014713014.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(1956..2741)
                     /locus_tag="VYN69_001832"
     CDS             complement(1956..2741)
                     /locus_tag="VYN69_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1534052.1"
                     /translation="MKKYQRMHLIFIRQYLKQIMEYKADFLVGVVGVFLTQGLNMLFL
                     NILFQHIPLLDGWSFHQVAFIYGFSLIPKGIDHLFFDNLWALGQHLIRKGEFDKYLTR
                     PISPLFHILVETFQIDALGELLVGVLLLLMTITSLTWTWAKVFLFLISIPFATLIYTS
                     LKIVTASIAFWTKQSGAIIYIFYMFNDFAKYPIAIYHSFLRWLISFIIPFAFTAYYPA
                     SYFLKDKDGLFNIGGLILISLIFFTLSLKLWNKGLDAYESAGS"
     gene            complement(2743..3560)
                     /locus_tag="VYN69_001833"
                     /pseudo
     CDS             complement(2743..3560)
                     /locus_tag="VYN69_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
     gene            complement(3562..4554)
                     /locus_tag="VYN69_001834"
                     /pseudo
     CDS             complement(3562..4554)
                     /locus_tag="VYN69_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
BASE COUNT         1744 a          950 c          790 g         1359 t
ORIGIN      
        1 aagttactaa aaaaagattt tggaataaac tttttcatag cgataaaagc accgtacaag
       61 ctaggaaaat aataagtaat cgcccaatct gaccttctcc agaacttgat tattcgtcta
      121 tcactacagt tgacaaacat actaagatta gtagtgagga aatctccaac gggagagagt
      181 actcattact ttttctttgt gttaaaatag tggtgtcttg ttaagtcata gagctctcta
      241 tggtgctaga cgtcgcttga caaactggca agaaacctgt tttaggaaga attttatcaa
      301 agtatgtggg atactagacg tcgagtattg gaaataaaat cactaaaaca aggagtcatt
      361 caagacaaag gggtaaacat gatgcggaca gttttcagaa tggatgtgag taaggcaagc
      421 tcagaagtcg ctattcttgt gaatggtgaa aaggttcatg gctataccat gcccaatgat
      481 gccatcggat ttagtcgtct tctagaggat ttgaaatagg taaatacacc tgagattatc
      541 ttcgaggcac aggggtctat tctcgtcttc aggcctttct ggaggacaat agctatgctt
      601 atatacggct aaatccttta aaactaagaa gcaactagac agtttgcgag tccgtaaaac
      661 caatcagatt gacgctgaaa agctagcttc gtcacaattc atccttaacc gcaaaccaac
      721 ttatgttcaa gaagaagtct atcaaaattt gcgtgacctc agtcgattct atcagaatct
      781 gacagaagac atcgttagga ctaagaatag cctgcacaag gtgcttcaaa ttgcctttcc
      841 agaaataaag aaccttctct ctactcctac aggggaacaa tattggaacc tcgtgaccat
      901 ctttccaacc aagcattgga ggctgaaatt atcagtagtt gagctaaaag aagccatcgt
      961 aattcaactt ctaaacggat ttctgagaac agagttggac gattggctga aaagttgagc
     1021 gagctggccg aacagtccta tcttgccatt tcaaaaacct ctcccatgac agaagaggtt
     1081 aagtatcatg cacaggaact gttgagactg acaaaccgca aggatcagga cttagcacaa
     1141 atgattgaat tggctgaacc attacctgaa tacgagattc tgctctctat tcctggtatc
     1201 gcagaaacaa cagctacaag cattattggc gaattggaga cattcgtcgc tttcagtctg
     1261 ccaaccaaat caatgccttt atcagtattg acctcagaca ctatgaatct ggcaatttcc
     1321 tagctcaaga acatatcacc aagcgtggaa atccttacgc gagaaaaatt ctctataagt
     1381 gcgtccacaa tattgcttca gctagtcata ccaatccttg tcatatcgcg gacttttatg
     1441 agaaacgaaa aagacaatcg cagacgactt caaccaagcc atatacgatt gtctctatac
     1501 atcgcctcat ccggacaatg tattacctca taacgcataa caaactttac gactacactt
     1561 taacccaaag tcattaaagt catctatgtc ctatcattgt aacaccttat tttttaatac
     1621 cagataagtg tcttttaagt gtgcccaaat ttcaaaaaac agctcgcaat tatcttctaa
     1681 acattccttt gatactcatt cagaaaacct tttgagctag aatatcgtat gtcccttaac
     1741 aaatggtatg tataaaagag ctaaaaaacc actcatgata tgaacgagtg gttatgactg
     1801 aaatattaca tacctttgta tcatttttgc acaatggtca aatttcttgt ttgaaaaact
     1861 acagtaagcc taatcaaagc tctcaatgta tcattaaaac ttccttccaa agaataggaa
     1921 gttctatcaa aattcttaat tagattttac ttcttctaag accctgcgct ctcatatgca
     1981 tccaaacctt tgttccataa ttttaatgat aaggtaaaaa atattaatga gataaggatc
     2041 aatccaccaa tattgaacaa tccatccttg tctttcaaga aatagctagc tgggtaatag
     2101 gctgtaaaag caaaaggaat gataaaacta atcaaccaac gtaaaaacga atggtaaatc
     2161 gcaatcgggt attttgcaaa atcattaaac atgtaaaaaa tataaattat tgccccagat
     2221 tgtttagtcc aaaaagcaat actagctgtt acaattttta aagaggtata aattaaggtt
     2281 gcaaagggaa tactgattaa aaagagaaaa acctttgccc aagtccaagt aagactagtt
     2341 attgtcatta acagcaataa gacgcctact aacaattctc caagagcatc aatttgaaac
     2401 gtttctacta aaatatgaaa caaagggcta atgggtctag tcaaatactt atcaaattct
     2461 cctttacgaa ttaaatgttg tcctaaagcc cacagattat caaaaaagag atggtcaatt
     2521 cccttaggaa tgagagaaaa accataaata aaagcaacct gatgaaaact ccagccatca
     2581 agtaagggaa tatgttgaaa caaaatattt agaaacagca tgtttaaccc ttgtgtcaaa
     2641 aagacaccca caacaccaac taaaaaatca gccttatact ccataatctg tttaagatat
     2701 tgtcggataa aaatcaggtg catgcgttga tatttcttca ttctaacctc cttgaatagt
     2761 tatataattc tgtacgcact tccaaatcag ttgggataag gcaatcataa taaacagcca
     2821 gaataattgt agcgagagcg cctgaatcat ttgactcatg ctatactttt ctataataac
     2881 catcaccggg gtatagatta aagaagaaaa tggcaaaaaa gataatagtt cggcaataat
     2941 ttttggaaaa aatgtcagag gaaccaaact tccagataaa aaggcaacta aagaattttt
     3001 caaaagattt gatccccata agtttttaaa cacaaaagct gaaaaaccaa agcaaatatt
     3061 gaaaaaaaat taatcaaaaa agctaagatt aagctaagaa ggtaaactgt ggttattaaa
     3121 acaatttgta agaaggactc acctgataag agctttagac ctgcaatcag aattacaaat
     3181 ggtagaccaa cactaacaaa aactagccat ctagacccaa tttctgtgaa aagataagac
     3241 attgcaaaat gaactggacg aagcagtcgc ataataatag agccatcctt tacttcccat
     3301 ccaatcataa aagaactatc tgattttgtc agtagattcg tcacaaaact catgatgata
     3361 tataaggtca tatcagataa agtaaaacct tgaatgagag actggtgaga tgaatcaaac
     3421 acagctttcc acaagtaaaa tgctacaaag gcccccatca catccccaat gcgataaaga
     3481 aaaaaattga cacgatacgt gattaattct tggattccag cgctaacaaa aggcttatag
     3541 cgtttccata atttagtcat cctaaagctc ctttcgatag aagcgacgaa taatatcttc
     3601 aatattcgca tccgtcatct ttaaatcacg gatcgtaaaa tcagacaaag tttgttgaat
     3661 aatgtcagcc gtttgatact gcgagctgtc gtactgaata tcaagtgaca attcctgtct
     3721 cgtcacatag atatcagaaa gtccttcaaa atgagagaca atataatcct gcccaggatg
     3781 tagttcaaaa gagagagtct tcatcttccc aaaagtcttt ttcagttgat tcacagttcc
     3841 atcaaagatt tctcggccct tatcaatcat aaaaatacga tcacataact gttcaatatc
     3901 acctaaatcg tgggtcgtta aaagtatggt cgtctcttct tcttgattaa tctgtgtaat
     3961 ggcacgtcga atattatcct tgaccgaaac atctaaacca atagtaggct catctaggaa
     4021 aagaactttc ggattatgca ataaggaggc cgcaatatcc gccctcatac gttgacctag
     4081 agatagagtt ctgacaggat cttgaataaa ttccttcaaa tctaaaactt catttagaaa
     4141 atccatgcgt ttttgaaaga ccgcatcagg aacatcataa atttccttta aaaccgagta
     4201 agtctcctac aaagccaaat cccaccaaag ttgagttcgc tgcccaaaaa ctacaccaat
     4261 atctttaaca tagccttgac ggttctcttg aggaatcttt ccattgatac gacaaaaacc
     4321 tgatgttggt ttcaaaatcc ccgtcaacat cttgatagtt gttgatttcc ccgctccatt
     4381 tgcgccaata aagcctaaaa tttgtccttt gggcacctca aatgataaat ctttaacagc
     4441 ttcaaagact tgtctcttgg gatgaaaaaa agagcgaaaa gctcccttta agccaggttc
     4501 ctttattgtt ttcacaaaat ttttctgaag atgctctact gttatcattt ttataaatca
     4561 ctcctttcgt ttcactcaag ttataatatc aacaaaaatt gtagcatact acagtaaagt
     4621 taagaaaatt taccatcatt acaaaccata tgaaaaaagc tactaaaact atgccatctt
     4681 gtattttatc tgtaagtaag ctaaattact caagtacaaa gaattatgac gaagaacata
     4741 taaatttata attggataaa ctatagattt cagcaaacga agttatccca atataaaaat
     4801 actttaataa ctgaagatat tgacaggaag atattaaatt cct
//
LOCUS       DAVLVP010000109         4784 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.109, whole genome shotgun sequence.
ACCESSION   DAVLVP010000109 DAVLVP010000000
VERSION     DAVLVP010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4784
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.109"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..244
                     /locus_tag="VYN69_001835"
     CDS             <1..244
                     /locus_tag="VYN69_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000945326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate uptake regulator PhoU"
                     /protein_id="HEV1534053.1"
                     /translation="SCYREEQSGCLDMVVIQKQNEIEELSINMEKKIMNYIFEDDGNV
                     SEVISALDIIHHLDKIAHTTQAIYKWIMYRKYGNIN"
     gene            257..1732
                     /gene="galT"
                     /locus_tag="VYN69_001836"
     CDS             257..1732
                     /gene="galT"
                     /locus_tag="VYN69_001836"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000848801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEV1534054.1"
                     /translation="MKTIIDNFVDRVIEYGMYNEIDKIYVKNRVLALIGEEGIDRISD
                     ENDLKQIKDYLVEIALKNGKIKDLIEEKECLGAELMNFIVPLPSRLNDIFWSSYDISP
                     QEAVEEFYKLSKDSDYIKTSAIAKNIEFRASTKYGELEITINLSKPEKDPKTIAAEKL
                     VKATNYPKCLLCMENEGYQGRINYPARSNHRIIRLKLGDEVWGFQYSPYSYFNEHAIF
                     LNSQHVPMAITSKTFEQLLEIVDILPGYFAGSNSDLPISGGSILSHNHYQGGKHIFPM
                     EKAKFESEFCFKDFEDVNAGIVKWPMSVIRLQSENKNRLLDLATKILNKWREYSDLEV
                     DIIAMTEDVPHHTVTPIARKVDGRYELDIVLRDNHTTEQYPDGVFHPHQDVQHIKKEN
                     IGLIEVMGLAILPPRLKPELEEVGKYLLGEDNAIADYHLEWADQLKEKYPRINKEEVN
                     SVVQHEAGQVFARVLEDAGVYKNTPSGHEAFMRFVKSVGIN"
     gene            1745..2755
                     /gene="galE"
                     /locus_tag="VYN69_001837"
     CDS             1745..2755
                     /gene="galE"
                     /locus_tag="VYN69_001837"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEV1534055.1"
                     /translation="MAILVTGGAGYIGSHTVVELLNLGKEVIIVDNLSNSSKLVLDRI
                     EAITGIRPVFYELDVCDKQALRKVFEQESIDAAIHFAGYKAVGESVQKPVMYYKNNIM
                     STLALVEVMSEFNVKKIVFSSSATVYGINNQSPLIETMQTSATNPYGYTKVMLEQILK
                     DVHVADSEWSIALLRYFNPIGAHESGLIGEDPSGIPNNLMPYIAQVAVGKLSELSVFG
                     NDYDTLDGTGVRDYIHVVDLAIGHIKALEKVSEKTDVYIYNLGSGEGTSVLQLVNTFE
                     SVNKIPIPYKIVPRRSGDVATCYANADKAYKELNWRTTKSIEDMCRDTWNWQSKNPNG
                     YN"
     gene            2935..3594
                     /locus_tag="VYN69_001838"
     CDS             2935..3594
                     /locus_tag="VYN69_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1868 domain-containing protein"
                     /protein_id="HEV1534056.1"
                     /translation="MKNLTKIKFKENGEFNHFPGNTVVANLYTKQDLMEVVDIIQSRY
                     RELPFIDKFTLTPRNSIHMTVIELLCHENRETEFWSSNLPLDTPLQEIHDYFAKQLEI
                     FPLLDEEIHMRITEMGKQNILVEPADEASAKRLEEIRTYVSEKAGVCFPNHDRYQFHI
                     SIGYLRIPLTEEEEEEFTKVRAELTEILLEKIPTITVNRIDYTVFEDMRQFVPYHEKF
                     K"
     gene            3619..4686
                     /locus_tag="VYN69_001839"
     CDS             3619..4686
                     /locus_tag="VYN69_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEV1534057.1"
                     /translation="MKKMKVWSTVLATGVALTTLAACSGGSNSTTASSSEEKADKSQE
                     LVIYSNSVSNGRGDWLTAKAKEAGFNIKMVDIAGAQLADRVIAEKNNAVADMVFGIGA
                     VDSNKIRDQKLLVQYKPKWLDKIDQSLSDKDNYYNPVIVQPLVLIGAPDVKEMPKDWT
                     ELGSKYKGKYSIYGLQGGTGRAILASILVRYLDEKGDLGVSEKGWEVAKEYFANAYTL
                     QKGESSVVKALDKESPIQYGMIWGSGALIGQKEQNVEFKVMTPEIGVPFVTEQTMVLS
                     TSKKQALAKEFIDWFGQSEIQVEYSKKFGSIPANKDALKELPEDTKKFVDQVKPQNID
                     WEAVGKHLDEWVEKAELEYVK"
BASE COUNT         1739 a          634 c          969 g         1442 t
ORIGIN      
        1 atcttgttat agagaggaac aatctggatg tttagatatg gttgttatac aaaaacagaa
       61 tgagatagaa gagcttagta taaatatgga aaagaaaata atgaattaca tatttgaaga
      121 tgatgggaac gtttctgaag taattagtgc tttagatatt attcatcacc ttgataaaat
      181 tgctcatact acgcaagcaa tttataaatg gataatgtat agaaaatatg ggaacataaa
      241 ttagaaagaa aaatatatga aaacaattat tgataatttt gttgatagag taattgaata
      301 cggaatgtat aatgaaattg acaaaatata tgtgaaaaat agagtactag cgttaatagg
      361 tgaggaggga attgatcgca tttccgatga aaatgacctg aaacaaataa aagattactt
      421 agtggaaatc gccttaaaaa acggtaaaat taaagattta atcgaggaaa aggaatgttt
      481 aggagcagag ttaatgaatt tcatagttcc tttacctagt cgattaaatg atattttttg
      541 gagttcatat gatatatcac ctcaagaagc agttgaagag ttttataagt taagtaaaga
      601 tagtgattat attaaaactt ctgctattgc taagaatatt gagtttcgag catcaactaa
      661 atacggagag ctagagatta cgataaattt gtcaaaaccc gaaaaagatc caaaaacaat
      721 tgctgctgaa aaattagtaa aagcaactaa ttatcccaaa tgtttactat gtatggaaaa
      781 tgaaggatat caggggcgaa taaattatcc agcacgttcg aatcatagaa ttattcggtt
      841 gaaacttggt gacgaagtat ggggattcca gtattctccc tactcatact ttaatgagca
      901 tgcgatattt ttgaatagtc aacatgtacc aatggctatt acctctaaaa catttgaaca
      961 attattggag attgttgata ttttgccagg atattttgct ggttcaaatt ctgatcttcc
     1021 tatctcagga ggctccattc ttagtcataa ccactaccag ggagggaaac atattttccc
     1081 aatggaaaaa gcaaaatttg agagtgaatt ttgttttaaa gactttgagg atgttaatgc
     1141 tggtattgta aaatggccaa tgtcagtaat taggttacaa agtgaaaata aaaatcgctt
     1201 gttagattta gctactaaaa ttctaaataa gtggagagaa tattcagatt tagaagtaga
     1261 catcattgct atgactgaag atgtcccaca ccatacggtg actcctattg ctcgtaaagt
     1321 ggacgggaga tacgaattag atattgttct aagagataat catactactg aacaataccc
     1381 agatggtgtt ttccatcctc atcaagatgt acaacatatt aaaaaggaaa atatcgggtt
     1441 aatcgaagtg atgggacttg cgatattacc acctcgtttg aaaccagagt tagaagaagt
     1501 tggaaaatat cttttaggag aggataatgc aattgcggat tatcatttag aatgggccga
     1561 ccaattgaaa gaaaaatatc ctcgcattaa taaagaagag gttaatagcg tagttcaaca
     1621 cgaagcagga caagtgtttg cgagagttct ggaagatgca ggagtttata agaatacacc
     1681 gtctgggcat gaagcgttta tgagatttgt taagtcggtt ggaattaatt aggaggaaac
     1741 ccagatggca atattggtaa caggcggagc tggttatatt ggtagccata ccgtagtaga
     1801 attactaaat ttaggaaagg aagttatcat tgtcgacaac ctttcgaact ctagcaaatt
     1861 ggtgctagac cgtattgaag caattacagg aatacgtccc gtgttttacg aattagatgt
     1921 ttgtgataaa caagcattga gaaaggtatt tgaacaagaa tcgattgatg ctgcaattca
     1981 ttttgcaggt tataaagctg tcggagaatc cgtgcaaaag cctgtgatgt actacaaaaa
     2041 taatattatg agtacattgg cacttgttga agtgatgtca gaatttaatg ttaaaaagat
     2101 tgtcttttct tcaagtgcga ctgtatatgg aattaacaat cagtcacctc taattgagac
     2161 gatgcaaaca agtgcgacaa atccttatgg gtatacgaaa gtgatgcttg agcaaatttt
     2221 aaaagatgtt catgtggcag attcagaatg gagtattgcg ttgcttcgtt atttcaatcc
     2281 aattggtgct catgagtctg gtttgattgg ggaagatccc tcaggaattc ctaacaactt
     2341 gatgccttat attgcacaag tagcggtagg taagctatca gagctaagtg tgttcggaaa
     2401 tgattatgat acgcttgatg gtactggtgt gcgtgattat atccatgtag tagatttagc
     2461 aataggacat ataaaagctt tagaaaaagt atctgaaaaa acagatgttt atatctataa
     2521 cctaggctcg ggagaaggca ctagtgtatt acaacttgta aatacatttg aaagtgttaa
     2581 taagatccct attccttata aaatagttcc aagacgttca ggagacgttg caacttgtta
     2641 tgcaaatgca gacaaagcat ataaggaatt aaattggagg acaacaaaat cgattgaaga
     2701 catgtgtaga gatacatgga attggcaatc aaaaaatccc aatggctata attgatgata
     2761 ctgtctatat gagtgctgta ttttggaaaa attacttaac cttttaatga aatgcatatt
     2821 taatgaaaga taaattaaaa aaaatgtttg acagcgctta acaaaggaaa cggtttcatt
     2881 gtataatgta aacataatga caccgatgtc aaatataaaa aagaaggtct ttatatgaaa
     2941 aacttaacaa aaattaagtt caaagagaat ggggaattta atcatttccc tgggaataca
     3001 gttgtagcaa atctttatac taaacaagat ttgatggaag ttgttgatat tattcaatca
     3061 cgttatagag aattaccatt tatcgataag tttactttaa ctccaaggaa ttctattcac
     3121 atgactgtaa ttgaattgtt gtgccatgaa aatcgtgaaa cggagttttg gagcagtaat
     3181 cttcctctag atacaccttt acaggaaata catgattact ttgctaaaca acttgaaatt
     3241 tttccattgt tggatgaaga aattcatatg cgtataactg aaatgggaaa acaaaacata
     3301 ctagttgaac ctgcagatga agcttctgca aagagattag aagaaattcg tacttatgtc
     3361 tcagaaaaag caggtgtttg tttccctaat catgatagat atcaattcca tatttcaatt
     3421 gggtatcttc ggattcctct aaccgaagag gaggaagaag agtttactaa agtcagagca
     3481 gaattaactg aaattttatt agagaagatt ccaactatta ctgtaaaccg tattgactat
     3541 actgtatttg aagatatgag acaatttgtt ccatatcacg aaaaatttaa ataaaataat
     3601 aaaaaaggag agcttatcat gaaaaaaatg aaagtttggt ctactgtact tgcaacggga
     3661 gttgctctta ctacacttgc tgcttgctct ggaggttcaa attctacgac tgcttcttca
     3721 tctgaagaaa aagctgataa aagtcaagaa ttagttatct attcgaactc agtctcaaat
     3781 ggtcgtggtg attggttaac tgctaaagca aaagaagctg gttttaatat aaaaatggtt
     3841 gatatcgctg gcgctcaatt agcagaccgt gttattgctg agaagaataa tgcagttgca
     3901 gatatggtat ttggaattgg tgctgttgac tcaaataaaa ttagagatca aaaattacta
     3961 gtacagtaca agcctaaatg gttagataaa attgatcaat ctttatcaga taaagataat
     4021 tattataatc ctgtgattgt tcaaccatta gttttaattg gggcgcctga tgtaaaagaa
     4081 atgcctaaag attggactga attaggtagt aagtataaag gtaaatattc aatttatggt
     4141 cttcaaggtg gtacaggacg ggcaattcta gcaagtatct tagttcgtta ccttgatgaa
     4201 aaaggagatt taggcgtatc agaaaaagga tgggaagttg caaaagaata ctttgcaaat
     4261 gcctatactc ttcaaaaagg tgaaagctca gttgttaaag cgttagataa agaaagccca
     4321 attcaatacg gtatgatctg gggttctgga gcgttaattg gacaaaaaga acaaaatgtt
     4381 gaattcaaag ttatgactcc tgagattggt gtaccatttg taactgaaca aactatggtt
     4441 ttaagcacta gtaaaaaaca agcgttagct aaagaattta ttgattggtt tggtcaatca
     4501 gaaattcaag tagaatatag taaaaaattt ggatctattc ctgccaataa agatgcccta
     4561 aaagagttac ctgaagatac gaaaaaattt gttgatcaag tgaaaccaca aaatattgac
     4621 tgggaagctg ttggaaagca tttggatgaa tgggtagaaa aagctgaatt agaatacgta
     4681 aaataagaaa taagagttat tttttatgtc caagtctctc tattatttct aaatttaagg
     4741 aagaaataaa gagaggcttt tggttttagt aatagaggga gtta
//
LOCUS       DAVLVP010000110         4687 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.110, whole genome shotgun sequence.
ACCESSION   DAVLVP010000110 DAVLVP010000000
VERSION     DAVLVP010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4687)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4687)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4687
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.110"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..1537
                     /locus_tag="VYN69_001840"
     rRNA            <1..1537
                     /locus_tag="VYN69_001840"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            1589..1661
                     /locus_tag="VYN69_001841"
     tRNA            1589..1661
                     /locus_tag="VYN69_001841"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1622..1624,aa:Ala,seq:tgc)
     gene            1786..4687
                     /locus_tag="VYN69_001842"
     rRNA            1786..4687
                     /locus_tag="VYN69_001842"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1290 a          965 c         1400 g         1032 t
ORIGIN      
        1 agtttgatcc tggctcagga cgaacgctgg cggcgtgcct aatacatgca agtagaacgc
       61 tgaaggagga gcttgcttct ctggatgagt tgcgaacggg tgagtaacgc gtaggtaacc
      121 tgcctggtag cgggggataa ctattggaaa cgatagctaa taccgcataa gagtggatgt
      181 tgcatgacat ttgcttaaaa ggtgcacttg catcactacc agatggacct gcgttgtatt
      241 agctagttgg tggggtaacg gctcaccaag gcgacgatac atagccgacc tgagagggtg
      301 atcggccaca ctgggactga gacacggccc agactcctac gggaggcagc agtagggaat
      361 cttcggcaat ggacggaagt ctgaccgagc aacgccgcgt gagtgaagaa ggttttcgga
      421 tcgtaaagct ctgttgtaag agaagaatga gtgtgagagt ggaaagttca cactgtgacg
      481 gtatcttacc agaaagggac ggctaactac gtgccagcag ccgcggtaat acgtaggtcc
      541 cgagcgttgt ccggatttat tgggcgtaaa gcgagcgcag gcggttagat aagtctgaag
      601 ttaaaggctg tggcttaacc atagtaggct ttggaaactg tttaacttga gtgcaagagg
      661 ggagagtgga attccatgtg tagcggtgaa atgcgtagat atatggagga acaccggtgg
      721 cgaaagcggc tctctggctt gtaactgacg ctgaggctcg aaagcgtggg gagcaaacag
      781 gattagatac cctggtagtc cacgctgtaa acgatgagtg ctaggtgtta gaccctttcc
      841 ggggtttagt gccgtagcta acgcattaag cactccgcct ggggagtacg accgcaaggt
      901 tgaaactcaa aggaattgac gggggcccgc acaagcggtg gagcatgtgg tttaattcga
      961 agcaacgcga agaaccttac caggtcttga catccctctg acgactctag agatagagtt
     1021 ttccttcggg acagaggtga caggtggtgc atggttgtcg tcagctcgtg tcgtgagatg
     1081 ttgggttaag tcccgcaacg agcgcaaccc ctattgttag ttgccatcat ttagttgggc
     1141 actctagcga gactgccggt aataaaccgg aggaaggtgg ggatgacgtc aaatcatcat
     1201 gccccttatg acctgggcta cacacgtgct acaatggctg gtacaacgag tcgcaagccg
     1261 gtgacggcaa gctaatctct taaagccagt ctcagttcgg attgtaggct gcaactcgcc
     1321 tacatgaagt cggaatcgct agtaatcgcg gatcagcacg ccgcggtgaa tacgttcccg
     1381 ggccttgtac acaccgcccg tcacaccacg agagtttgta acacccgaag tcggtgaggt
     1441 aaccgtaagg agccagccgc ctaaggtggg atagatgatt ggggtgaagt cgtaacaagg
     1501 tagccgtatc ggaaggtgcg gctggatcac ctcctttcta aggataagga actgcgcatt
     1561 ggtcttgttt agtcttgaga ggtcttgtgg ggccttagct cagctgggag agcgcctgct
     1621 ttgcacgcag gaggtcagcg gttcgatccc gctaggctcc attggtgaga gatcaccaag
     1681 taatgcacat tgaaaattga atatctatat caaatagtaa caagaaaata aaccgaaaac
     1741 gctgtagtat taataagagt ttatgactga aaggtcagaa aataaggtta agttaataag
     1801 ggcgcacggt ggatgccttg gcactaggag ccgacgaagg acgtgacaaa cgacgatatg
     1861 ccttgggtag ctgtaagtaa gcgatgatcc agggatttcc gaatggggga acccaacagg
     1921 tagtacctgt tacccacatc tgttaaggat gtgaggagga agacgcagtg aactgaaaca
     1981 tctaagtagc tgcaggaaga gaaagcaaaa gcgattgcct tagtagcggc gagcgaaacg
     2041 gcagaagggc aaaccgaaga gtttactctt cggggttgta ggactgcaat gtggactcaa
     2101 agattataga agaatgattt gggaagatca gccaaagaga gtaatagcct cgtatttaaa
     2161 atagtctttg tacttagcag tatcctgagt acggcgggac acgtgaaatc ccgtcggaat
     2221 ctgggaggac catctcccaa ccctaaatac tccctagtga ccgatagtga accagtaccg
     2281 tgagggaaag gtgaaaagca ccccgggagg ggagtgaaat agaacctgaa accgtgtgcc
     2341 tacaacaagt tcgagcccgt taatgggtga gagcgtgcct tttgtagaat gaaccggcga
     2401 gttacgttat gatgcgaggt taagttgaag agacggagcc gtagggaaac cgagtctgaa
     2461 tagggcgcct tagtatcatg acgtagaccc gaaaccatgt gacctaccca tgagcaggtt
     2521 gaaggtgcgg taagacgcac tggaggaccg aaccagggca cgttgaaaag tgcttggatg
     2581 acttgtgggt agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata
     2641 gctttagggc tagcgtcgac attagagatt cttggaggta gagcactgtt tgggtgaggg
     2701 gtccatcccg gattaccaat ctcagataaa ctccgaatgc caatgaatta tggtcggcag
     2761 tcagactgcg agtgctaaga tccgtagtcg aaagggaaac agcccagacc accagctaag
     2821 gtcccaaaat aattgttaag tggaaaagga tgtggggttg cacagacaac taggatgtta
     2881 gcttagaagc agctattcat tcaaagagtg cgtaatagct cactagtcga gtgaccctgc
     2941 gccgaaaatg taccggggct aaaacaattt accgaagctg tggatacctt tataggtatg
     3001 gtaggagagc gttctatgtg tgatgaaggt ataccgtgag gagtgctgga acgcatagaa
     3061 gtgagaatgc cggtatgagt agcgaaagac aggtgagaat cctgtccacc gtaagactaa
     3121 ggtttccagg ggaaggctcg tccgccctgg gttagtcggg acctaaggag agaccgaaag
     3181 gtgtatccga tggacaacag gttgatattc ctgtactaga gtatgtagtg atggagggac
     3241 gcagtaggct aactaaagca gacgattgga agagtctgtc taagcagtga ggtgtgaatt
     3301 gagtcaaatg cttaattcta taacattgag ctgtgatggg gagcgaagtt tagtagcgaa
     3361 gttagtgacg tcacactgcc aagaaaagct tctagcgttt aaacatactc tacccgtacc
     3421 gcaaaccgac acaggtagtc gaggcgagta gcctcaggtg agcgagagaa ctctcgttaa
     3481 ggaactcggc aaaatgaccc cgtaacttcg ggagaagggg tgctgacttt aagtcagccg
     3541 cagtgaatag gcccaagcaa ctgtttatca aaaacacagc tctctgctaa atcgtaagat
     3601 gatgtatagg gggtgacgcc tgcccggtgc tggaaggtta agaggagtgc ttagcgtaag
     3661 cgaaggtatg aattgaagcc ccagtaaacg gcggccgtaa ctataacggt cctaaggtag
     3721 cgaaattcct tgtcgggtaa gttccgaccc gcacgaaagg cgtaatgatt tgggcactgt
     3781 ctcaacgaga gactcggtga aattttagta cctgtgaaga tgcaggttac ccgcgacagg
     3841 acggaaagac cccatggagc tttactgcag tttgatattg agtgtctgta ccacatgtac
     3901 aggataggta ggagtctaag agatcgggac gccagtttcg aaggagacgc tgttgggata
     3961 ctacccttgt gttatggcca ctctaaccca gataggtgat ccctatcgga gacagtgtct
     4021 gacgggcagt ttgactgggg cggtcgcctc ctaaaaggta acggaggcgc ccaaaggttc
     4081 cctcagaatg gttggaaatc attcgcagag tgtaaaggta taagggagct tgactgcgag
     4141 agctacaact cgagcaggga cgaaagtcgg gcttagtgat ccggtggttc cgtatggaag
     4201 ggccatcgct caacggataa aagctaccct ggggataaca ggcttatctc ccccaagagt
     4261 tcacatcgac ggggaggttt ggcacctcga tgtcggctcg tcgcatcctg gggctgtagt
     4321 cggtcccaag ggttgggctg ttcgcccatt aaagcggcac gcgagctggg ttcagaacgt
     4381 cgtgagacag ttcggtccct atccgtcgcg ggcgtaggaa atttgagagg atctgctcct
     4441 agtacgagag gaccagagtg gacttaccgc tggtgtacca gttgtcttgc caaaggcatc
     4501 gctgggtagc tatgtaggga agggataaac gctgaaagca tctaagtgtg aaacccacct
     4561 caagatgaga tttcccatga ttatatatca gtaagagccc tgagagatga tcaggtagat
     4621 aggttagaag tggaagtgtg gcgacacatg tagcggacta atactaatag ctcgaggact
     4681 tatccaa
//
LOCUS       DAVLVP010000111         4507 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.111, whole genome shotgun sequence.
ACCESSION   DAVLVP010000111 DAVLVP010000000
VERSION     DAVLVP010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4507
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.111"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..658)
                     /locus_tag="VYN69_001843"
     CDS             complement(<1..658)
                     /locus_tag="VYN69_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000917571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1534058.1"
                     /translation="MLKQEKLAKILEIVNSKGTITVKQIMDEIAVSDMTARRYLQELA
                     DKDLLIRVHGGAEKLRTNSLLTNERSNIEKQALQTAEKQEIAHFAGSLVEERETIFIG
                     PGTTLEFFARELPIDNIRVVTNSLPVFLILSERKLTDLILIGGNYRDITGAFVGTLTL
                     QNLSNLQFSKAFVSCNGIQNGALATFSEEEGEAQRIALNNSNKKYLLADHSKFNKFDF
                     Y"
     gene            complement(840..1802)
                     /gene="nrdF"
                     /locus_tag="VYN69_001844"
     CDS             complement(840..1802)
                     /gene="nrdF"
                     /locus_tag="VYN69_001844"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004253599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEV1534059.1"
                     /translation="METYYKAINWNAIEDVIDKSTWEKLTEQFWLDTRIPLSNDLDDW
                     RKLSNKEKDLVGKVFGGLTLLDTMQSETGVQALRADIRTPHEEAVFNNIQFMESVHAK
                     SYSSIFSTLNTKAEIEEIFEWTNTNPYLQKKAEIVNEIYLNGSPLEKKVASVFLETFL
                     FYSGFFTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEEEQEKLK
                     EWMYDLLYTLYENEEGYTESLYDGVGWTEEVKTFLRYNANKALMNMGQDPLFPDSAED
                     VNPIVMNGISTGTSNHDFFSQVGNGYLLGEVEAMQDDDYNYGLD"
     gene            complement(1990..4149)
                     /gene="nrdE"
                     /locus_tag="VYN69_001845"
     CDS             complement(1990..4149)
                     /gene="nrdE"
                     /locus_tag="VYN69_001845"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000523276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEV1534060.1"
                     /translation="MGLKHLEDVTYFRLNNEINRPVNGQIMLHKDKEALDAFFKENVV
                     PNTMVFDSIKDKINYLIEHNYIETAFIKKYHPEFLEELAQFIKDQNFQFKSFMAAYKF
                     YNQYALKTNDGEYYLENMEDRVFFNALYFADGNEAVAIDIANEIIHQRYQPATPSFLN
                     AGRARRGELVSCFLIQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSTKKENADEKVR
                     VKTLSLGVVVPDKFYELARKNEEMYLFSPYSVEKEYGVPFNYIDITEKYDELVANPNI
                     RKTKIKARDLETEISKLQQESGYPYVVNIDTANRANPVDGKIIMSNLCSEILQVQEPS
                     LINDAQEFLQMGTDVSCNLGSTNVVNMMTSPDFGRSIRAMVRALTFVTDSSHIVAVPT
                     IDHGNSQAHTFGLGAMGLHSYLAQQLIEYGSPESVEFTSIYFMLMNYWTLVESNNIAR
                     ERGITFHNFEKSDYANGSYFDKYVTGEFVPTSDRVKELFKNVFIPGVADWAELRDKVQ
                     EDGLYHQNRLAVAPNGSISYINDVSASIHPITQRIEERQEKKIGKIYYPAAGLSTETI
                     PYYTSAYDMDMRKVIDVYAAATEHVDQGLSLTLFMRSDIPKGLYEWKRENKQTTRDLS
                     ILRNYAFNKGIKSIYYVRTFTDDGGEVGANQCESCVI"
     gene            complement(4230..4448)
                     /gene="nrdH"
                     /locus_tag="VYN69_001846"
     CDS             complement(4230..4448)
                     /gene="nrdH"
                     /locus_tag="VYN69_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000259243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEV1534061.1"
                     /translation="MVTVYSKNNCVQCKMTKRFLDSNNVSYREINLDEQPEYVDQVKE
                     LGFSAAPVIQTPTEVFSGFQPGKLKQLA"
BASE COUNT         1310 a          840 c          920 g         1437 t
ORIGIN      
        1 tataaaaatc aaacttattg aacttgctat gatctgcgag taaatatttt ttattagaat
       61 tatttaaagc gatgcgttga gcctctccct cttcctcgct aaaagtagct agagctccgt
      121 tttgaatacc attacagcta acgaaagctt tagaaaattg gagattagag agattttgta
      181 gggtcaatgt accaacaaaa gcacctgtaa tatcgcgata atttccacct attaaaatca
      241 aatctgttaa ttttcgttcg cttaaaatca gaaaaacagg tagactgttg gttacgacgc
      301 ggatattgtc aataggcaac tcacgcgcaa aaaactctaa tgttgttcct ggtccaatga
      361 aaatagtttc tctttcttct actagactgc ctgcaaaatg ggctatttct tgtttttctg
      421 ccgtttggag ggcttgtttt tcaatatttg atcgctcatt agtcaaaagg gagttggttc
      481 gaagtttttc agctccacca tgcacacgaa tcagcaaatc tttatcagct aattcctgta
      541 aatagcgcct tgcagtcata tctgaaacgg ctatttcgtc cataatctgt ttaactgtta
      601 tggttccttt actatttact atctctaaaa ttttggctaa tttttcttgt ttgagcatat
      661 tatcacctcg tttcctacta ctatcttacc ataaacaaac tcatcattca aatacaaaaa
      721 caacaaaatg aaacaaaaac aaaaatatcg aagtttgttt tcaaaacttt cgatattttt
      781 gttgggttat aactttgatg tttctagttt actttttgat gattgagagt gatggagaat
      841 tagtctaaac cgtagttata gtcatcgtct tgcatggctt caacttcgcc aagaaggtaa
      901 ccatttccga cttgagagaa gaagtcatgg ttggaagttc ctgttgaaat accgttcata
      961 acgattgggt tgacatcttc agctgaatct gggaaaagtg gatcttgtcc catgttcatg
     1021 agagctttat tggcattgta gcgaaggaag gttttaacct cttcagtcca accaacaccg
     1081 tcataaagac tctctgtgta gccttcttca ttttcataaa gagtatagag taggtcgtac
     1141 atccattctt tgagtttttc ttgctcttct tcaggcaatt cattgaaacc aagttggaat
     1201 ttgtaaccaa tgtaggttcc gtgaacagac tcgtcacgaa taatcaattt aatgatttct
     1261 gcaacgttgg ctagtttgtt gttaccgaga tagtagaggg gagtgaagaa accagagtag
     1321 aagaggaagg tttcgaggaa gacgctggca actttctttt caagtgggct gccgtttagg
     1381 tagatttcgt tgacaatctc agccttcttt tgtaggtaag gattggtatt ggtccattcg
     1441 aaaatttctt caatctcagc cttagtattc aaggtagaaa agattgatga gtaagattta
     1501 gcgtggacag attccataaa ttggatgtta ttgaaaacag cttcctcatg tggtgtacgg
     1561 atgtctgcgc gaagggcttg aaccccagtt tcagattgca tagtgtcaag aagggttaaa
     1621 ccaccaaaaa cttttccgac caagtctttc tctttgttag atagctttct ccagtcatcc
     1681 aagtcgtttg ataagggaat acgtgtatcg agccaaaatt gctccgtcag tttttcccaa
     1741 gttgatttgt cgatgacatc ttcgatggca ttccagttaa tggctttgta gtaagtttcc
     1801 atttaaaatc tctttctgtg tttagtattg cgaactcaca attatttcca ctttaccata
     1861 attctatagg agtatcgcac aaaaagtcgg aagcccgact tttaaaatgt tacataaatt
     1921 atgttatgac atagtagatt tgattttatc agtgctgctt agggaaaaat aatgtttcta
     1981 tgctagaaac taaatcacac agctttcaca ttggttggcg ccgacttctc caccgtcatc
     2041 tgtaaaggta cggacgtagt agatagactt gattcccttg ttaaaggcat agttacgaag
     2101 gatggacaag tcacgtgtcg tttgtttatt ttccctcttc cattcgtaaa ggccttttgg
     2161 aatgtcacta cgcatgaaga gggtgagtga aagtccttga tccacgtgtt cagtcgcagc
     2221 agcgtaaaca tcgatgactt tacgcatatc catatcgtag gcagaagtgt agtaaggaat
     2281 ggtttctgta gacaagccag cagcagggta gtagatttta ccaattttct tctcttggcg
     2341 ttcttcgata cgttgcgtaa tcgggtggat agaagcagaa acgtcgttga tatagctgat
     2401 agaaccattt ggcgctacag caaggcgatt ttggtggtaa agaccatctt cttgaacctt
     2461 gtcgcgaagt tcagcccaat cagcaacacc agggataaag acatttttga agagttcttt
     2521 aacacggtct gatgttggaa caaattcacc agttacatac ttgtcaaagt aacttccgtt
     2581 agcatagtct gatttttcaa agttgtggaa ggtaatacca cgttcacgcg caatattgtt
     2641 tgactctacc aaggtccagt agttcataag cataaagtag atgcttgtaa attcaacaga
     2701 ctcaggtgaa ccatattcaa tgagttgttg ggcaaggtag ctgtgcagtc ccatggcacc
     2761 gagaccaaag gtgtgggctt ggctatttcc atggtcaatc gttggtacag ctacgatatg
     2821 tgaactatct gtaacgaaag taagggcacg aaccatagca cggatagaac gaccaaaatc
     2881 aggtgaagtc atcatgttaa ccacgttggt tgaacccagg ttacatgaaa catctgttcc
     2941 catttgaagg aattcttgag catcgttgat caagcttggt tcttgaactt gaagaatctc
     3001 agaacacaag ttactcatga taatctttcc atcaacagga tttgcacggt tagccgtatc
     3061 gatgttgact acataaggat agccagactc ttgttgcaat ttagagattt cagtttccaa
     3121 atcccgcgcc ttgatttttg tcttgcgaat atttggattt gcgaccaact catcgtattt
     3181 ttcagtaatg tcgatgtaat tgaatggcac accgtattct ttttctacag agtaagggct
     3241 gaagaggtac atttcttcat ttttacgagc caattcgtag aatttatcag gtactacaac
     3301 accaagtgat agagtcttga cacgtacttt ttcatcagcg ttttctttct tagttgaaag
     3361 gaaagcgatg atatctgggt gaaagacgtt gaggtagaca acaccggcac cttgacgttg
     3421 ccccaattgg ttggagtaag agaagctgtc ttcaaaaagc ttcataacag gaacgacacc
     3481 tgaagcagct ccttcatagc ctttgatagg tgcaccagct tcacgaaggt tgctgagggt
     3541 aattcccaca ccaccaccaa tacgtgaaag ttgaagagct gagttgatag aacgtccgat
     3601 agagttcata tcatccgtca cttggattag gaaacaagat accaactctc cacggcgagc
     3661 acgtccagca ttcaagaagg aaggagtagc aggttggtag cgttggtgga tgatttcatt
     3721 ggcaatatcg attgcaacag cttcattccc atcagcgaaa taaagggcgt tgaagaagac
     3781 acggtcttcc atattttcaa gatagtattc accgtcatta gtctttaagg catattgatt
     3841 gtaaaattta taagctgcca tgaatgactt gaattggaag ttttggtctt tgataaattg
     3901 agctaattct tccaagaact ctggatggta tttcttgata aaggctgttt cgatgtagtt
     3961 gtgttcaatg aggtaattga ttttgtcttt gattgaatca aaaaccatag tgtttggaac
     4021 tacattttct ttaaagaaag catccaaggc ttccttgtct ttatgaagca tgatttgtcc
     4081 attaacagga cggttaattt cgttattaag acggaagtaa gtcacgtctt caagatgttt
     4141 taatcccata aaatttccct tatctaatta caaaagaaag gcttctaagt tagccctaaa
     4201 agcagtttct tctggatgat gtactaagat tatgctaatt gtttcagttt tcctggttgg
     4261 aaacctgaaa agacttcagt tggtgtttgg ataacaggag ctgcgctaaa accgagctct
     4321 ttaacttgat cgacgtactc aggttgctca tcaagattga tttcacgata agagacatta
     4381 ttactgtcca agaaacgctt ggtcatttta cattggacac aattgttttt agaataaacg
     4441 gttaccattg tgtaactcct cttcaaaatt taatactatc ttagtatatc agaaaataaa
     4501 attttgt
//
LOCUS       DAVLVP010000112         4454 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.112, whole genome shotgun sequence.
ACCESSION   DAVLVP010000112 DAVLVP010000000
VERSION     DAVLVP010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.112"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            115..414
                     /gene="yajC"
                     /locus_tag="VYN69_001847"
     CDS             115..414
                     /gene="yajC"
                     /locus_tag="VYN69_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001069053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEV1534062.1"
                     /translation="MNPNITFLIMLVGMMALMFFMQRSQKKQAQKRMESLNKLQKGYE
                     VITIGGLYGTVDEVDTEKGTIVLDVDGVYLTFELAAIKTVLPLKETASLEGAIEK"
     gene            461..889
                     /locus_tag="VYN69_001848"
     CDS             461..889
                     /locus_tag="VYN69_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000737444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEV1534063.1"
                     /translation="MKKLVFVCLGNICRSPMAEFVMKSMTDNYEIQSRATSSWEHGNP
                     IHKGTQGIFQQYEIPYDKNKTSLQISKEDFEAFDYIIGMDASNISDLRQMCPVDCQDK
                     IYSFSSESVPDPWYTGDFEETYRRVQEGCQAWLERLEKES"
     gene            891..1301
                     /locus_tag="VYN69_001849"
     CDS             891..1301
                     /locus_tag="VYN69_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534064.1"
                     /translation="MENLTNFYEKYRVYLTRPRLELLAVVTIVFCAVLVFFLNIPGKG
                     VLKLDNGTIVYDGSLVRGKMNGQGTITFQNGDQYTGGFNNGAFNGKGTFQSKEGWTYE
                     GDFVNGQAEGKGKLTTEQEVVYEGTFKQGVFQQK"
     gene            1601..4252
                     /gene="adhE"
                     /locus_tag="VYN69_001850"
                     /gene_synonym="adhC"
     CDS             1601..4252
                     /gene="adhE"
                     /locus_tag="VYN69_001850"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000763984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEV1534065.1"
                     /translation="MADKKTVTPEEKKLVAEKHVDELVQKALVALEEMRKLDQEQVDY
                     IVAKASVAALDAHGELALHAFEETGRGVFEDKATKNLFACEHVVNNMRHTKTVGVIEE
                     DDVTGLTLIAEPVGVVCGITPTTNPTSTAIFKSLISLKTRNPIVFAFHPSAQESSAHA
                     ARIVRDAAIAAGAPENCVQWITQPSMEATSALMNHEGVATILATGGNAMVKAAYSCGK
                     PALGVGAGNVPAYVEKSANIRQAAHDIVMSKSFDNGMVCASEQAVIIDKEIYDEFVAE
                     FKSYHTYFVNKKEKALLEEFCFGVKANSKNCAGAKLNADIVGKPATWIAEQAGFTVPE
                     GTNILAAECKEVGENEPLTREKLSPVIAVLKSESREDGITKARQMVEFNGLGHSAAIH
                     TADEELTKEFGKAVKAIRVICNSPSTFGGIGDVYNAFLPSLTLGCGSYGRNSVGDNVS
                     AINLLNIKKVGRRRNNMQWMKLPSKTYFERDSIQYLQKCRDVERVMIVTDHAMVELGF
                     LDRIIEQLDLRRNKVVYQIFADVEPDPDITTVNRGTEIMRAFKPDTIIALGGGSPMDA
                     AKVMWLFYEQPEVDFRDLVQKFMDIRKRAFKFPLLGKKTKFIAIPTTSGTGSEVTPFA
                     VISDKANNRKYPIADYSLTPTVAIVDPALVLTVPGFVAADTGMDVLTHATEAYVSQMA
                     SDYTDGLALQAIKLVFENLESSVKNADFHSREKMHNASTIAGMAFANAFLGISHSMAH
                     KIGAQFHTIHGRTNAILLPYVIRYNGTRPAKTATWPKYNYYRADEKYQDIARMLGLPA
                     STPEEGVESYAKAVYELGERIGIQMNFRDQGIDEKEWKEHSRELAFLAYEDQCSPANP
                     RLPMVDHMQEIIEDAYYGYKERPGRRK"
BASE COUNT         1464 a          849 c          920 g         1221 t
ORIGIN      
        1 ccaaaaatca gggcgtaagc tctggttttt cttaccagaa aagtaaggta caatcttgta
       61 aaagtagctg aaatttgata tagtagtcct atgtaaaaga caaaggagaa aacaatgaat
      121 ccaaatatta cttttttaat catgcttgta ggtatgatgg ccttgatgtt ctttatgcaa
      181 cgttctcaaa agaaacaagc tcaaaagcgt atggaaagct taaataaact acaaaaaggc
      241 tatgaagtga ttacaatcgg tggactttat ggaacagtcg atgaagtaga tacggagaag
      301 ggaacaatag ttcttgacgt agatggagtt tacttgactt ttgaactagc tgctatcaag
      361 acagtattac cgctgaaaga aacagcttca ctagaaggcg caattgaaaa ataagacggg
      421 atcacgaact cccgtttttc tataaaagaa aggaaatggg atgaaaaaat tagtctttgt
      481 ctgtctggga aatatttgcc gtagccctat ggccgagttt gttatgaaat caatgacaga
      541 taactacgaa atccaaagtc gagcaacttc ctcttgggaa catggcaatc cgattcataa
      601 ggggactcag ggaatttttc aacagtatga gattccttat gacaagaaca agacatcgct
      661 tcagattagt aaggaagatt ttgaagcctt tgattatatt atcggaatgg acgcttcaaa
      721 tatttctgac ttacgtcaga tgtgtccagt agactgtcaa gataagattt actcattttc
      781 atctgaaagt gttccagacc cttggtatac aggagatttt gaagaaacct atcgacgtgt
      841 tcaagagggc tgtcaagctt ggttagaacg tttagaaaag gagagttaag gtggaaaatc
      901 ttacgaattt ttacgaaaag tatcgtgtct atctgactcg tccacgttta gagcttttgg
      961 cagtagttac cattgttttc tgtgctgtac tcgtcttttt tctaaatatt ccaggaaaag
     1021 gtgtcttaaa actcgataat ggaacgattg tttatgatgg cagtcttgtt cgtggtaaaa
     1081 tgaatggcca aggtaccatt accttccaaa atggagacca atatacaggt ggcttcaaca
     1141 atggagcctt caacggaaaa ggtacctttc aatctaaaga aggctggacc tacgaaggtg
     1201 attttgtaaa tggtcaggct gaaggaaaag ggaaactaac aacagaacaa gaagtcgttt
     1261 atgaaggaac ttttaaacaa ggcgtttttc aacaaaaata aagcctcctt atcaaaggag
     1321 gtattattag aatcacaagg taagcgctta cctgtaaatc cctttctttc caaatccctc
     1381 ttccaagcaa gtttgtgaaa taaaaaatat ttgaaataaa tttcacaaac ttcaaagata
     1441 aaacttgata agaaaagaaa atgagaaaag tttcacaaga gtttaaaaat tctttgtgaa
     1501 atgtttatga aactgtttac aaagttgaaa taatgcgtta taataaactt gtgaaaaaat
     1561 taacaaagga tatcgttcct tgaaagctat ggaggaaaat atggctgata aaaaaactgt
     1621 gacaccagag gaaaagaaac tcgttgctga aaaacacgta gatgagttgg ttcaaaaagc
     1681 tctagttgcc cttgaagaaa tgcgtaaatt ggatcaagaa caagttgact acatcgttgc
     1741 caaagcatca gtagcagctt tggatgccca cggagaattg gctttacatg cctttgaaga
     1801 aacaggacgt ggtgtatttg aagacaaagc aactaagaac ttgtttgctt gtgaacacgt
     1861 agtaaacaac atgcgccaca ctaagacagt tggcgttatc gaagaagacg atgtaacagg
     1921 attgactctt attgctgaac cagttggtgt tgtttgtggt attactccaa caacaaaccc
     1981 aacatcaaca gcaatcttca aatcattgat ttcattgaag acacgtaacc caatcgtctt
     2041 tgccttccat ccatcagcac aagaatcatc tgctcatgca gctcgtatcg tccgcgatgc
     2101 agctatcgca gctggtgctc ctgaaaactg tgtgcaatgg attactcaac catctatgga
     2161 agcaacaagt gctcttatga accacgaagg tgttgcgaca atccttgcaa caggtggtaa
     2221 tgccatggtt aaggcggctt attcatgtgg taaaccagct cttggggtag gtgccggaaa
     2281 cgttccagct tatgttgaaa aatcagcaaa cattcgtcaa gcggcacacg atatcgtcat
     2341 gtctaaatca tttgataacg gtatggtctg tgcatctgaa caagcagtta tcattgataa
     2401 agaaatttac gatgaatttg tagcagagtt caaatcttac cacacttact ttgtaaacaa
     2461 aaaagaaaaa gctcttcttg aagagttctg cttcggcgtc aaagcaaaca gcaaaaactg
     2521 tgctggtgca aaattgaacg ctgacatcgt tggtaaacca gcaacttgga ttgcagaaca
     2581 agcaggattt acagttccag aaggaacaaa tattcttgct gcagaatgta aagaagttgg
     2641 cgaaaatgag ccattgactc gtgaaaaatt gtcaccagtt attgcagttt tgaaatctga
     2701 aagccgtgaa gatggtatta ctaaggctcg tcaaatggtt gaatttaacg gtcttggaca
     2761 ctcagcagct atccacacag ctgacgaaga attgactaaa gaatttggta aagctgttaa
     2821 agctattcgt gttatctgta actcaccttc tacttttggt ggtatcgggg acgtttacaa
     2881 tgccttcttg ccatcattga cacttggatg tggttcttac ggacgcaact cagttgggga
     2941 taacgttagt gccattaacc tcttgaatat caaaaaagtc ggaagacgga gaaataacat
     3001 gcaatggatg aaacttcctt caaaaacata ctttgaacgt gattcaattc aataccttca
     3061 aaaatgtcgt gacgttgaac gtgtcatgat cgttactgac catgccatgg tagagcttgg
     3121 tttccttgat cgtatcatcg aacaactgga ccttcgtcgc aataaggttg tttaccaaat
     3181 ctttgcggat gtagaaccgg atccagatat cacaactgta aaccgtggta ctgagattat
     3241 gcgtgccttc aaaccagata ccatcatcgc actcggtggt gggtctccaa tggatgctgc
     3301 caaagtaatg tggctcttct acgagcaacc agaagtggac ttccgtgacc ttgtccaaaa
     3361 attcatggat atccgtaaac gtgccttcaa gttcccattg cttggtaaga agactaaatt
     3421 catcgcgatt ccaactacat ctggtacagg atctgaagta acaccatttg ccgttatctc
     3481 tgataaagca aacaaccgta aatacccaat cgctgactac tcattgacac caactgtggc
     3541 aatcgtagat cctgctttgg tattgacagt tccaggattt gttgctgctg atactggtat
     3601 ggacgtattg actcacgcga cagaagcata cgtatcacaa atggctagtg actacactga
     3661 tggtttagca cttcaagcca ttaaattggt ctttgaaaat ctcgaaagct cagttaagaa
     3721 tgcagacttc cactcacgtg agaaaatgca taacgcttca acaatcgctg gtatggcctt
     3781 tgccaatgcc ttcctaggta tttctcactc aatggcccat aagattggtg cgcaattcca
     3841 cacaatccac ggtcgtacaa atgctatctt gcttccatac gttatccgtt acaacggtac
     3901 acgtccagct aagacagcaa catggcctaa gtacaactac taccgtgcag atgaaaaata
     3961 ccaagatatc gcacgcatgc ttggacttcc agcttctact ccagaagaag gggttgaatc
     4021 ttacgcaaaa gctgtctacg aactcggtga acgtattggg atccaaatga attttagaga
     4081 ccaaggaatt gacgaaaaag aatggaaaga acattctcgt gaattagcct tcctggctta
     4141 tgaagaccaa tgttcaccag ctaacccacg tcttccaatg gtagaccata tgcaagaaat
     4201 catcgaagat gcatactatg gctacaaaga aagaccagga cgccgtaaat aattgtttat
     4261 cagtctagaa gcaagacaaa aactcaattt gagggaaaga tccagtaatt tttctatgat
     4321 aaaaggcatc ctatcaaggt ttttgaacac ctgataggat gcctttttat gatattgagg
     4381 cctttttgcc ctttttgaaa aactagaata gaaacaaaat atataataga ttgaaactgg
     4441 aatagtacac ttct
//
LOCUS       DAVLVP010000113         4414 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.113, whole genome shotgun sequence.
ACCESSION   DAVLVP010000113 DAVLVP010000000
VERSION     DAVLVP010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4414
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.113"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            9..1271
                     /locus_tag="VYN69_001851"
     CDS             9..1271
                     /locus_tag="VYN69_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000413340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1534066.1"
                     /translation="MEKLLQEKLLPVAARLGNNKALVSIRDGITLTIPLLLIGSLLMV
                     IASFPIPGWEKYLGDIGVADYLWKGVDSSFGLLGLVASFGIAYFMARQYKVDGIPAGI
                     VSLSSFITVTPFIRGEAGAGMPTAFMASKGLFVAMILGLINGYIYQWFINHNIQIKMP
                     DGVPPAVSKSFSAIIPGAVTIVGWLIVYATLDKLSLPNLHEIAQVALGGPLGLLGNNV
                     IGLLILIFLNSSFWFVGLHGGNVVNAVMKPLWLANLDANKVAYQTGETLPNIFTSVFM
                     DNFVFIGGGGATIGLVLALGYLAHKKKASKQLKTLAPITVIPGLFNINEPAMFGVPIV
                     LNILLLVPFILAPMFNLLVAWGAMASGLVPLTYTDPGWTMPPVISGLLATGSISGSLL
                     QIVLIVLDVLLYLPFVIAIEKRFKLLED"
     gene            1274..2170
                     /locus_tag="VYN69_001852"
     CDS             1274..2170
                     /locus_tag="VYN69_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEV1534067.1"
                     /translation="MKRLISANPSEILQMNAEELKQSILASEGRVVLSENVVTRETFV
                     GDITNSEIARAFGADMILLNCVDVFEPKIYALDSSGDDVIHRLHQLVACPIGVNLEPI
                     DPSAKMLEETQEIVAGRVASVETLNRIEELGFDFVCLTGNPGTGVSNREIIKAVQTAK
                     ENFSGLIIAGKMHGAGVNEPVAELSVAEQLLEAGADVILVPAVGTVPAFHDQELREVV
                     DLVHSKGGLVLSAIGTSQETSDTDTIKEIALRNKICGVDIQHIGDAGYGGLATVDNIY
                     ALSKAIRGVRHTVSRLARSVNR"
     gene            2185..2502
                     /locus_tag="VYN69_001853"
     CDS             2185..2502
                     /locus_tag="VYN69_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001151647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1534068.1"
                     /translation="MAKVTIMLACAAGMSTSLLVTKMQKAAEDKGLDAEIFAVPAPEA
                     EEIVATKEVNVLLLGPQVRYLLGDFQEKLKDRQIPVAVIPMTDYGMMNGSKVLDLAES
                     LLD"
     gene            2519..2836
                     /locus_tag="VYN69_001854"
     CDS             2519..2836
                     /locus_tag="VYN69_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1534069.1"
                     /translation="MNESNLESVMGLIMYGGEAKSNAMEAIQAAKKGDFSKANRRLAD
                     ANAALLQAHKAQTEMLTREAQGEKTSISLLMVHAQDHLMTSLTFVDLAKEVVEVYERF
                     EKN"
     gene            2846..4201
                     /locus_tag="VYN69_001855"
     CDS             2846..4201
                     /locus_tag="VYN69_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1534070.1"
                     /translation="MNTMLDKMQEKLSPIAMKVENQKFLVALRDSFVGTMPVIMTGSI
                     ALLLNAFLVDLPQQFHLESITKTFQWLVDINNLVFKGSIPIVSLLFIYCLGVNIAKIY
                     KVDTVSAGLVSLASFVISIGSTVTKSFPLANVGDVKLDQILQGIDNLAFDGKNLMVTI
                     GNVIPGNHINARGYFTAMMIGFLASIIFCKVMKKNWVIKLPDSVPPAIAKPFTSIIPG
                     FMAMYIVAILTYVFHLLSNDLLIDWVYKVLQTPLLGLSQSFFAVILMIFLNKLFWFFG
                     LHGGNVLAPIMEGLFGVAMLANLDAFQKGEPIPYIWTSGSFGAFVWFGGLGLVLAILI
                     FSRNSHYRKVAKLGLAPVLFNIGEPVNYGLPVVLNPLLFIPFVLSPVFMATVAYWATS
                     WGLVSPVTQNVTWVMPPILYGFFSTAFDWRAIILSVVCLIISVLTYFPFVKMADKTEL
                     S"
BASE COUNT         1290 a          622 c          985 g         1517 t
ORIGIN      
        1 aaaaggttat ggaaaaatta ttacaagaaa agttacttcc agtagcggct agattgggta
       61 ataataaagc gttggtttct attcgagacg gtattactct aactatacct ttattgttga
      121 taggttcatt actaatggtt attgcaagtt ttcctattcc aggatgggaa aaatatctgg
      181 gtgatattgg tgttgctgac tatttatgga agggtgttga tagtagtttt ggattacttg
      241 gtcttgtagc tagttttggt atcgcttact ttatggctag acaatacaaa gttgatggta
      301 ttcctgctgg tattgtgtca ttatcatctt ttattacagt tacgcctttc attagaggag
      361 aggctggagc aggaatgcct accgctttca tggcatcaaa aggtttattt gttgctatga
      421 ttttaggatt gattaatggc tatatctacc aatggtttat aaatcataat atacagataa
      481 aaatgccgga tggtgttcca ccagcagtat ctaaaagctt tagtgccatt attcctggtg
      541 cagtgactat tgttggttgg ttgattgttt atgcaacttt agataaatta agtttaccta
      601 atcttcatga aattgctcaa gtagctttgg gaggtccact tggactttta ggaaataatg
      661 ttattggtct tcttatctta attttcctta atagtagttt ttggtttgta ggattacacg
      721 gaggaaatgt tgttaatgca gttatgaaac cgttatggtt agctaatcta gatgcaaata
      781 aagtagctta tcaaactgga gaaactcttc caaatatttt tactagcgta tttatggata
      841 attttgtatt cataggtggc ggtggtgcaa ccataggttt agttcttgcg ttaggatatt
      901 tagcacataa gaagaaagct agtaaacaac taaaaacttt ggcacctata actgttatac
      961 ctggattatt taatataaat gaaccagcaa tgtttggcgt tcctattgtt ttgaacattt
     1021 tgttactagt tccatttatc ttagctccaa tgtttaactt actagtagcg tggggagcaa
     1081 tggcatcagg tttggttccg ctaacttata cagatccagg ttggactatg ccacctgtta
     1141 taagtggttt acttgctaca ggaagtattt ctggttcatt attacaaatt gtattgattg
     1201 ttttggatgt cttactttat ttaccatttg taatagctat tgaaaaacgg tttaagttat
     1261 tggaggattg agaatgaaac gtttaattag tgcaaatcca tctgagatat tacagatgaa
     1321 tgctgaagaa ttaaaacaaa gtattttagc aagtgaaggt agagttgttc tatctgaaaa
     1381 tgtagttact cgtgagacat ttgttgggga tataactaat tctgaaattg ctagagcttt
     1441 tggagctgat atgattttat tgaattgtgt tgatgttttt gagcctaaaa tttatgcttt
     1501 ggatagttca ggtgatgatg ttattcatcg cttacaccag cttgttgctt gtccaattgg
     1561 tgtaaatttg gaaccgattg acccatctgc aaagatgcta gaggaaacac aggaaattgt
     1621 tgcaggtcgt gttgctagtg ttgaaacatt gaatcgaata gaggagttag gttttgactt
     1681 tgtctgtttg actggaaatc ctggaacagg agttagcaat cgagaaatca ttaaggctgt
     1741 tcaaactgct aaggaaaact tttctggttt gattattgca ggtaagatgc acggggcagg
     1801 agtgaatgag cctgtggcag agctttctgt cgcagagcaa ttgttggaag caggtgcgga
     1861 tgtgatactt gttccagcag ttggaaccgt tccagccttt catgaccaag agttgcgtga
     1921 agtcgttgat ctcgttcata gtaagggtgg tcttgtattg agtgctattg gtactagcca
     1981 agaaacatct gatacagata ctattaagga aattgcactt agaaataaaa tttgtggagt
     2041 tgatattcaa catataggtg acgcaggata tgggggactg gcaacagtcg ataatattta
     2101 tgcattgagc aaggcaatta gaggagtgag acatacagta tctcgcttgg ctaggtcagt
     2161 aaataggtga taaaggagta agctatggct aaagtaacaa ttatgttagc atgtgcagca
     2221 ggtatgagta caagtctgct agtgacaaag atgcaaaagg cagcagaaga taaggggttg
     2281 gatgcagaaa tttttgcagt tccagctcct gaagcagaag aaattgtagc aacaaaagaa
     2341 gtaaatgtgt tgcttttagg ccctcaagtt cgctatttac taggggactt tcaagaaaaa
     2401 ctaaaagata gacagattcc tgtggcggtt attccgatga cagattatgg aatgatgaat
     2461 ggttctaaag tcttagattt agctgaaagt ttattagact aagattgagg agaatgctat
     2521 gaatgaaagt aatttagaat ctgtaatggg gctaattatg tatggtgggg aagccaaaag
     2581 taatgctatg gaggctattc aggcagcaaa aaaaggggat ttctctaaag ccaatcgaag
     2641 attagctgat gcgaatgctg ccttattaca ggcgcataag gctcaaacag aaatgttgac
     2701 aagagaggca cagggggaaa aaacatcaat tagcctcttg atggtacacg cgcaagatca
     2761 tttaatgacg agtctgactt ttgttgactt agcaaaagaa gtggtagaag tatacgaacg
     2821 atttgaaaaa aattaggagt ttggtatgaa tacaatgttg gataaaatgc aagaaaaact
     2881 ttctccaatt gcaatgaaag ttgagaatca gaagttttta gttgctttac gtgattcatt
     2941 tgtggggact atgcctgtta ttatgacagg ttccattgct ttgttattaa atgcttttct
     3001 agtggattta ccacaacaat ttcacctaga aagtattacg aaaacctttc agtggcttgt
     3061 tgacatcaat aatttggtat tcaagggaag tataccaatt gtttccttgt tgttcatcta
     3121 ttgcttggga gtgaatatcg ctaagattta taaggttgat acagtttctg ctggtttggt
     3181 atcgcttgct tcgtttgtca tttcgattgg aagtacagtt acaaagagtt tccctttggc
     3241 aaatgtagga gatgtgaaat tagatcaaat cttacaagga attgataatc tagcatttga
     3301 tggtaaaaat ctgatggtaa ctattgggaa tgtgattcca ggaaaccata ttaatgccag
     3361 aggctacttt acagccatga tgattggatt tttagcctct attattttct gtaaagtaat
     3421 gaaaaagaac tgggtaatca agttaccaga ttcagttccc cctgcgattg ctaagccgtt
     3481 cacttctatc attccaggat ttatggcgat gtatatagta gctatcttga cttatgtttt
     3541 ccatttactt agcaatgact tattgattga ttgggtttac aaggtgttac aaacgccatt
     3601 actaggtttg tctcaaagtt tctttgcagt tattctgatg atctttttga ataagttatt
     3661 ctggtttttt ggtctccatg gaggaaatgt attggctccc atcatggaag ggctgtttgg
     3721 agttgctatg ttagcgaatc tggatgcttt ccaaaaaggt gaaccgattc cttatatatg
     3781 gacaagtggg tcttttgggg cgtttgtctg gtttggagga ttaggattag tacttgctat
     3841 tttaattttt tcaagaaaca gtcattatcg aaaagttgct aaacttggtc tagcaccagt
     3901 tctgtttaat attggtgagc cggtcaatta tggtttacca gttgtcttga atcctttgct
     3961 atttatacca tttgtactta gcccggtttt catggcaacg gtagcttact gggcaacaag
     4021 ctggggtctt gtttcacctg ttacacaaaa tgttacttgg gtaatgcctc caattttata
     4081 tggtttcttc tctacagcat ttgattggcg tgccatcatc ttatcagttg tttgtttgat
     4141 tattagtgtc ttgacttatt tcccatttgt gaaaatggca gataaaactg aattgagtta
     4201 atggtcttac ttgcgttttt tgtgataaaa tagaaataga ttacgtgtaa gatgagttag
     4261 gtgtaggatt tcttatctcc actaaaattg ttaatagaca aaggtttata gtttattgaa
     4321 gttggaatag tacactgtaa cttctaaaac agtactagaa aatgatttga atttcctaat
     4381 cgatttagtc atattttagt tcatttttat ataa
//
LOCUS       DAVLVP010000114         4392 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.114, whole genome shotgun sequence.
ACCESSION   DAVLVP010000114 DAVLVP010000000
VERSION     DAVLVP010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4392
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.114"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            113..1447
                     /gene="pepC"
                     /locus_tag="VYN69_001856"
     CDS             113..1447
                     /gene="pepC"
                     /locus_tag="VYN69_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000991632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase C"
                     /protein_id="HEV1534071.1"
                     /translation="MNAIQESFTDKLFANYEANVKYQAIENAASHNGIFAALECRQSH
                     VDNTPVFSLDLTKDKVTNQKASGRCWMFAALNTFRHKLISQYKLENFELSQAHTFFWD
                     KYEKSNWFLEQVIATSDQELTSRKVSFLLQTPQQDGGQWDMVVSLFEKYGVVPKSVYP
                     ESVSSSSSRELNAILNKLLRQDAQILRDLLVSGADQATVQAKKEDLLQEIFNFLAMSL
                     GLPPRKFDFAYRDKDNNYKSEKGITPQEFYKKYVNLPLEDYVSVINAPTADKPYGKSY
                     TVEMLGNVVGSRAVRYINVPMERLKELAIAQMQAGETVWFGSDVGQLSNRKAGILATD
                     VYDFESSMDIKLTQDKAGRLDYSESLMTHAMVLTGVDLDENGKSTKWKVENSWGDKVG
                     TDGYFVASDAWMDEYTYQIVVRKELLTAEEQAAYGAEPIVLAPWDPMGALAE"
     gene            complement(1503..2414)
                     /locus_tag="VYN69_001857"
     CDS             complement(1503..2414)
                     /locus_tag="VYN69_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEV1534072.1"
                     /translation="MTEKLQLTKSDRKKVWWRSTFLQGSWNFERMQNLGWAYTLIPAI
                     KKLYTKKEDQIAALERHLEFFNTHPYVAAPVMGVTLALEEERANGVEIDDAAIQGVKI
                     GMMGPLAGIGDPVFWFTVRPILGSLGASLALTGNILGPLLFFVAWNLIRMSFLWYVQE
                     IGYKAGSEITKDMSGGILQDITKGASILGMFILAVLVQRWVNIKFAFDVSKVQLDEKA
                     YIHWDKLPEGSKGIQEAFAQVGQGLSQTPEKVTTFQQNLDMLIPGLSGLLLTLLCMYL
                     LKKKVSPITIILALFAVGIVAHVLHIM"
     gene            complement(2438..3241)
                     /locus_tag="VYN69_001858"
     CDS             complement(2438..3241)
                     /locus_tag="VYN69_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEV1534073.1"
                     /translation="MSIISMVLVVVVAFFAGLEGILDQFQFHQPLVACTLIGLVTGHL
                     EAGIILGGSLQMIALGWSNIGAAIAPDAALASVAAAIIMVLGGDFTKTGIGVAQAVAI
                     PLAVAGLFLTMIVRTISVGLVHTADAAAKKGDFGAVERAHFIALLFQGLRIALPAALL
                     LMVPTETVQSILSAMPDWLKDGMAIGGGMVVAVGYAMVINMMATREVWPFFALGFVLA
                     AVSDITLIGFGAIGVAIALIYLHLSKTGGNGGGGAATSNDPIGDILEDY"
     gene            complement(3269..4258)
                     /gene="manX"
                     /locus_tag="VYN69_001859"
     CDS             complement(3269..4258)
                     /gene="manX"
                     /locus_tag="VYN69_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000021981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEV1534074.1"
                     /translation="MSIGIIIASHGEFAAGIHQSGSMIFGEQEKVQVVTFMPNEGPDD
                     LYAKFNNAVAAFDAEDEVLVLADLWSGSPFNQASRVMGENPERKFAIITGLNLPMLIQ
                     AYTERLMDAAAGVEKVAANIIKEAKDGIKALPEELNPVEEVASAAAAPVAQTAIPEGT
                     VIGDGKLKINLARLDTRLLHGQVATAWTPDSKANRIIVASDNVAKDDLRKELIKQAAP
                     GNVKANVVPIQKLIEISKDPRFGETHALILFETPQDALRAIEGGVPIKTLNVGSMAHS
                     TGKTLVNTVLSMDKEDVATFEKMRDLGVEFDVRKVPNDSKKDLFDLINKANVK"
BASE COUNT         1334 a          919 c          935 g         1204 t
ORIGIN      
        1 gaagagtata aattcgaaaa aggtgaggga caatgtcctc gccttttatg ttttttagtt
       61 gcttcctttg tgaaaagagt tataatagac tgtagaataa aaggaggaat ctatgaacgc
      121 gattcaagaa tcatttactg ataaactatt tgccaactat gaagcaaatg taaaatacca
      181 agcgattgaa aatgctgcca gccacaacgg aatttttgca gctctggaat gtcgccaaag
      241 ccatgtagac aacacacctg ttttctcatt ggatttaacc aaggacaagg tcactaacca
      301 gaaagcgtct ggtcgttgct ggatgtttgc ggctctcaac accttccgcc acaaactcat
      361 ctcgcaatac aaattggaga actttgagtt gtcacaagcc cacactttct tctgggataa
      421 gtatgagaaa tcaaactggt tcttggagca agtcattgcg acttcagacc aagaattgac
      481 tagccgcaag gttagcttct tactccaaac acctcaacaa gatggtggtc aatgggatat
      541 ggtcgtttcc ctctttgaaa aatacggtgt cgtgcctaag tcagtttatc ctgagtctgt
      601 ttcatctagc agcagtcgtg agctaaatgc gatccttaat aaattgcttc gtcaagatgc
      661 tcaaatcttg cgtgacttgc ttgtttctgg tgcagatcaa gcgactgttc aagctaagaa
      721 agaagacctc ttgcaagaaa tctttaactt tcttgctatg tcattaggac ttccaccacg
      781 caagtttgac tttgcttatc gcgataaaga taacaactac aaaagtgaaa aaggaatcac
      841 accacaagag ttttacaaga aatatgtcaa tcttccttta gaagactacg tttctgttat
      901 caatgctcca actgctgata aaccttacgg aaaatcttac acagttgaga tgttggggaa
      961 tgtggttggt agccgtgcag ttcgctacat caacgttcca atggagcgct tgaaagaatt
     1021 ggcgattgcc caaatgcaag caggtgagac tgtttggttt ggttctgatg tcggccagct
     1081 cagcaaccgt aaggctggca tccttgcgac agatgtttat gactttgaat caagcatgga
     1141 cattaaactt actcaagaca aggctggacg tttggactat agtgaaagct tgatgaccca
     1201 cgccatggtc ttgacaggtg ttgacttgga cgaaaatggt aaatcaacca agtggaaggt
     1261 tgaaaactca tggggagaca aggtcggtac agatggttac tttgttgcct cagacgcttg
     1321 gatggacgaa tacacatacc aaatcgttgt tcgtaaggaa ttgctgacag cagaagaaca
     1381 agctgcctat ggagcagaac caatcgtact tgcaccatgg gatccaatgg gagccttggc
     1441 tgaataaaag catagaaaaa aggaatcaga ttttagaacc tggttccttt ttagttgctt
     1501 gattacatga tgtgaagaac atgtgccaca atacccactg cgaagagggc aaggataata
     1561 gtgattggag atactttttt cttaagtaag tacatgcaaa gtaaagtaag gagtagtcct
     1621 gataatccag gaatcaacat atccaagttt tgttggaaag tagtaacttt ttcaggagtt
     1681 tgagacaatc cttgtcctac ttgtgcgaat gcttcttgga tacctttaga cccttctggc
     1741 aatttatccc aatggatata agccttttca tctagttgaa ctttagaaac atcgaaagca
     1801 aatttaatat ttacccagcg ttgaacaagg acagcaagaa tgaacatccc aaggatagaa
     1861 gctcctttag tgatatcttg aaggatacca ccagacatat ctttagtgat ttctgatcca
     1921 gccttgtatc caatctcttg aacataccac aagaatgaca tacgaatcaa gttccatgca
     1981 acaaagaaga ggagtggacc caagatattg ccagtaaggg caagtgaagc accgagagat
     2041 ccaaggattg ggcgtactgt aaaccagaat actgggtcac cgataccagc aagaggtccc
     2101 atcataccga ttttaacccc ttggatagca gcgtcatcga tttccacacc gttagcacgt
     2161 tcttcttcaa gcgcaagagt aacccccatg actggagcag ctacgtatgg atgagtgttg
     2221 aagaactcaa ggtgacgctc aagagcagcg atttgatctt cttttttagt atagagtttt
     2281 ttgatagctg gaatgagtgt ataagcccag cccaagtttt gcatccgttc aaagttccaa
     2341 gacccttgta agaaggttga acgccaccaa acttttttac gatctgattt agttaattga
     2401 agtttttcag tcatgatgtt ttcagtcctt tcttatctta gtagtcttct aggatatcgc
     2461 cgattgggtc gttagaagtt gcggctcctc cgccaccatt tccaccagtt ttagaaaggt
     2521 gaaggtagat aagagcgata gcaacaccga tagcaccgaa tccgattaga gtaatatctg
     2581 acacagcagc gagaacgaaa ccaagagcga agaatggcca tacttcacga gttgccatca
     2641 tgttgataac catggcgtaa ccaacggcaa cgaccatacc accaccgata gccataccat
     2701 ctttgagcca gtctggcatg gcactaagga tactttgtac agtttcagtt ggtaccataa
     2761 ggagaagagc tgcaggaagc gcgatacgaa gtccttggaa aagtagcgca atgaaatgag
     2821 cacgctccac agcgccgaag tcaccttttt tagcggcagc atcagcagtg tgaaccaatc
     2881 caactgaaat tgtacgaaca atcatagtta agaatagacc agctacggca agagggatag
     2941 caaccgcttg ggcaacaccg ataccagtct tggtaaagtc accaccaaga accatgataa
     3001 tggcagcagc gacagaagca agtgcagcat caggagcgat agcagcaccg atatttgacc
     3061 aaccaagggc aatcatttga agcgatccac cgaggataat ccctgcttcc aagtgacctg
     3121 ttacaagccc aataagggta caggctacaa gtggttggtg aaattggaac tggtcgagga
     3181 tgccttcaag acctgcaaag aaggctacaa cgactactaa aaccatagaa ataatagaca
     3241 tgtttaaaat cctttcataa ataatggctt atttgacatt ggctttgtta atcaagtcaa
     3301 acaaatcttt tttagaatca tttggtactt tacggacatc aaattcaaca cccaagtcac
     3361 gcattttttc aaatgtagca acatcttctt tgtccataga caaaacggta ttgaccaatg
     3421 ttttacctgt tgagtgagcc atagaaccaa cgttaagagt cttgattggc acgccgcctt
     3481 cgatggcacg aagggcatct tgaggtgttt caaacaagat aagggcatgt gtttctccaa
     3541 aacgtgggtc ttttgaaatc tcaatcagtt tttgaattgg aaccacgtta gccttgacat
     3601 tacctggagc tgcttgttta atcaattctt tacgaaggtc gtctttagcc acgttatctg
     3661 aagcaacaat gatacgattt gcttttgaat ctggagtcca agcagttgca acctgaccgt
     3721 gaagtagacg tgtgtcaaga cgggcaagat tgattttcaa tttaccgtct ccgataacag
     3781 ttccttctgg gatagcagtt tgggcaactg gagcagctgc agcgcttgca acttcttcaa
     3841 ctggatttag ctcttctgga agagctttga tgccatcttt ggcttcttta atgatattag
     3901 cagcgacttt ttctacacct gcagcagcgt ccatgaggcg ctctgtgtag gcttgaatca
     3961 acatcggtaa gttaagtcct gtgatgatgg caaacttacg ctcaggattt tctcccatca
     4021 cgcgactagc ttggttaaat ggagaaccac tccaaaggtc agccaaaact agaacctcat
     4081 cttctgcgtc aaatgcagca acagcgttat taaacttagc gtatagatca tcaggacctt
     4141 catttggcat aaaggtcaca acttgaacct tttcttgttc accaaagatc atagatcctg
     4201 actgatgaat acccgcagca aattcgccgt ggctcgcaat aatgattccg atactcatta
     4261 ttgtcattcc tccttttttg ttttagtttt catttaagaa aactttaaga ctgtttaagt
     4321 ataaaccgtt ttcataaaaa aagcaactat ttatcataaa aaattaaaaa atttttattt
     4381 ttgtatgttg at
//
LOCUS       DAVLVP010000115         4310 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.115, whole genome shotgun sequence.
ACCESSION   DAVLVP010000115 DAVLVP010000000
VERSION     DAVLVP010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4310
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.115"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            1..168
                     /locus_tag="VYN69_001860"
                     /pseudo
     CDS             1..168
                     /locus_tag="VYN69_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            335..535
                     /locus_tag="VYN69_001861"
     CDS             335..535
                     /locus_tag="VYN69_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534075.1"
                     /translation="MVASASASSTSTQAQEQVDKSELRALSQELDQRLKALATISDPK
                     IDATKAVLLDAQKAPEDSALTE"
     gene            577..981
                     /locus_tag="VYN69_001862"
     CDS             577..981
                     /locus_tag="VYN69_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534076.1"
                     /translation="MDSLKDVKANASDSKPAQDKKDAKQGTEDSKDSDKMTETNSVPA
                     GVIVVSLLALLGVIAFWLIRRKKESEIQQLSTELIKVLGQLDAEKADKKVLAKAQNLL
                     QETLDFVKEENGSAETETKLVEELKAILDKLK"
     gene            1212..1919
                     /gene="ccdA2"
                     /locus_tag="VYN69_001863"
     CDS             1212..1919
                     /gene="ccdA2"
                     /locus_tag="VYN69_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004237834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     membrane protein CcdA2"
                     /protein_id="HEV1534077.1"
                     /translation="METIVFLISVFLAGVLSFFSPCIFPLLPVYAGILLDDQESAKSF
                     SLFGRKVLWSGLIRTLCFIAGISLIFFILGFGAGYFGHILYANWFRYGMGAIIIILGL
                     HQMEIFHLKKLEVQKSFTFKKSDSNRYWSAFLLGITFSFGWTPCIGPVLSSVLALAAS
                     GGNGAWQGAIYTLIYTLGMALPFLVLALASGLVMPYFSKIKRHMMLLKKIGGFLIVLM
                     GILLLLGQVNVLAGIFE"
     gene            1930..2487
                     /gene="sdbB"
                     /locus_tag="VYN69_001864"
     CDS             1930..2487
                     /gene="sdbB"
                     /locus_tag="VYN69_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000757364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase-associated
                     lipoprotein SdbB"
                     /protein_id="HEV1534078.1"
                     /translation="MKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQIA
                     VGKDAPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDF
                     EILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDS
                     QGKIGKIQFGAISNADAEAAFKEMN"
     gene            2861..4252
                     /locus_tag="VYN69_001865"
     CDS             2861..4252
                     /locus_tag="VYN69_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001020339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEV1534079.1"
                     /translation="MNIFRTKNVSLDKTEMHRHLKLWDLILLGIGAMVGTGVFTITGT
                     AAATLAGPALVISIVISALCVGLSALFFAEFASRVPATGGAYSYLYAILGEFPAWLAG
                     WLTMMEFMTAISGVASGWAAYFKGLLSQYGIAFPQALNGTFNPQAGTFVDLLPILVLV
                     LVTSLVLLNAKAALRFNSILVILKFSALALFVLVGIWNIKFDNWSNFAPYGFGQIYGA
                     STGIMAGASLMFFGFLGFESISMAVDEVKTPQKNIPRGIVLSLSIVTILYALVTLVLT
                     GVVHYSHLNVDDAVAFALRSVGISWAANYVSLVAILTLITVCISMTYALSRMIYSLAR
                     DGLVPAAFKELTKTSKIPKNATILTGLASAVAAGMFPLASIAAFLNICTLAYLIMLAY
                     GLIRLRKEKGMPKAGEFKTPLVPLLPILSIIICLSFMLQYNMNTWIAFLVALLVGSII
                     YFTYGYKHSTIEE"
BASE COUNT         1260 a          780 c          914 g         1356 t
ORIGIN      
        1 aaagtatctg gaagaagata tcagaggatt tctttggttg caggtctaac aaatggtgaa
       61 ttaatcgctc caatgactta cgaagagatg atgacgagcg acttttttga agtatggttt
      121 cagaagtttt tcttaccaac attaaccaca ccatcggtta ttatagtaaa atgaaataag
      181 aataggacaa atcgatcagg acagtaaaat cgaattctaa caatgtttta gaagtccata
      241 tgtactattc tagtttcaat ataccataac tgttcctagt caacctgcaa gccctgtccc
      301 agctccaagc tcagctccga ctcaagatcc tagagtggta gcaagcgctt cggctagctc
      361 aacgagcaca caagctcaag agcaagttga caagtctgaa cttcgtgcct tgagtcaaga
      421 gttagaccaa cgcttgaaag ctttggcaac aatatctgat ccgaaaatcg atgcaaccaa
      481 ggctgtcctc ctagatgctc aaaaagctcc ggaagatagt gccttgacag agtaaggtct
      541 gcatactgca gtagagtctg tgaaggctgg gctgccttgg actctctcaa agatgtgaaa
      601 gcaaatgcta gcgacagcaa gcctgcacag gacaagaagg atgcaaaaca aggaacggaa
      661 gatagtaagg attcagataa gatgactgaa acaaactcag ttccggcagg agtgattgtg
      721 gtcagtctac ttgccctcct aggcgtgatt gccttctggc tgattcgccg taagaaagag
      781 tcagaaatcc agcaattaag cacggaattg atcaaggttc taggacagct agatgcagaa
      841 aaagcggata aaaaagtcct tgccaaagcc caaaaccttc tccaagaaac ccttgatttc
      901 gtgaaagaag aaaatggctc agcagagaca gaaactaaac tagtagagga gcttaaagca
      961 atccttgaca aactcaagta aaacatgatt tatgaaatgt cagacctaaa agacattttc
     1021 gttcacaaaa gcagcgatga gtaatccttg tcgttgcttt ttggctattt tgggggataa
     1081 gatccgcctt gactagcaaa agaaaagatt ggtccaatag tgcccgatac gattttgcgt
     1141 agcagaaagc ttacaaataa aattgaatat agtaaaatag gattagaatt attaagaaag
     1201 ttggttcttt attggaaacg atagtatttt taatctctgt ttttctagca ggtgttttat
     1261 cctttttttc tccttgtatt tttcctcttc tgccagtcta tgctgggatt ttattggatg
     1321 atcaagaaag tgcaaaaagc ttttctttgt ttgggagaaa ggttctctgg tcaggcttga
     1381 ttcgaacact ttgctttatc gctggtatct ctctcatttt ctttattcta ggctttggtg
     1441 ctggttactt tggtcatatt ctctatgcaa attggtttcg atatggcatg ggagctatta
     1501 ttatcatttt gggtcttcac cagatggaaa tttttcattt gaagaaatta gaagttcaaa
     1561 aaagttttac ctttaaaaaa tcagattcta atcgttattg gtcagctttt ttacttggta
     1621 ttacctttag ctttggttgg acaccttgta ttggtccagt tttaagttct gttttagcac
     1681 ttgcggcttc tggaggcaat ggcgcttggc aaggagcgat ttatactctc atttacactc
     1741 tgggcatggc ccttcctttc ttggtattgg cactagcttc aggtctagtc atgccatatt
     1801 ttagtaaaat caagcgtcat atgatgctac taaagaaaat tggtggtttc ctcattgttt
     1861 taatgggaat tttgttacta ttaggacaag taaatgttct agctggaatt tttgaataaa
     1921 ggaggacaaa tgaaaaaagt aatgtttgct ggcttaagtc tcttgtcatt agttgtattg
     1981 atggcctgtg gtgaggaaga aactaaaaag actcaagcag cacaacagcc aaaacaacaa
     2041 acgactgtac aacaaattgc tgttggaaaa gatgctccag acttcacatt gcaatccatg
     2101 gatggcaaag aagttaagtt atctgatttt aagggtaaaa aggtttactt gaagttttgg
     2161 gcttcatggt gtggtccatg caagaaaagt atgccagagt tgatggaact agcggcgaaa
     2221 ccagatcgtg atttcgaaat tcttactgtc attgcaccag gaattcaagg tgaaaaaact
     2281 gttgagcaat tcccacaatg gttccaggaa caaggatata aggatatccc agttctttat
     2341 gataccaaag caaccacctt ccaagcttat caaattcgaa gcattcctac agaatattta
     2401 attgatagcc aaggaaagat tggaaagatt caatttggtg ctatcagtaa tgcggatgca
     2461 gaagcagcat ttaaagaaat gaactagaat cgataaaatc tgattacaag atgtagtcag
     2521 attttggtaa cttctaaaac taacttccag tttcccacaa cctagctttt agtatgagaa
     2581 aaacgcgtga catcagtgga aatccgtctt agactaactt ccactggttt tctttttctt
     2641 gatatataag ggttcaaaag cgaaaagact cagaaaaagt gcacgacaaa tagccctaaa
     2701 acagagtcat ctgagactaa cttccagttt tgggagagag atggaagtta ctttgagaag
     2761 ttacggtagt cagatttttt tagaaaaaca ttttataaat attcacatat ctcctatatt
     2821 tatggtaaaa tagaatcatc agtttatttt ggagtcaaag atgaatatat ttagaacaaa
     2881 gaatgttagt ttagataaaa cagagatgca taggcatttg aagttatggg atttgatttt
     2941 gctgggtatc ggagccatgg tagggacagg cgtctttaca atcacaggta ctgcagctgc
     3001 aacacttgct ggcccagccc tagtgatttc aatcgttatt tctgccttgt gtgtgggatt
     3061 atcagccctc ttttttgcag aatttgcctc gcgagtaccc gctacaggag gtgcctatag
     3121 ttacctctat gctatcttag gagaattccc tgcctggttg gctggttggt taaccatgat
     3181 ggagttcatg acagccatat caggcgtagc ttcgggttgg gcagcttatt ttaaaggctt
     3241 gctctctcaa tacgggatag cctttcctca ggctttaaat ggtaccttta atcctcaagc
     3301 agggacattt gttgatttat tgcctattct tgtcttggtc ttggtgactt cgcttgtttt
     3361 actaaatgcc aaggcagcct tacgctttaa ttcgattcta gttattttga aattttccgc
     3421 tttagctctc tttgttttag taggaatttg gaatattaag tttgataatt ggagcaattt
     3481 tgctccttac ggttttggac aaatctatgg agctagcact ggtattatgg ctggtgcgtc
     3541 cttgatgttc ttcggttttt tgggatttga atccatctct atggctgtag atgaagtcaa
     3601 gactcctcaa aaaaatattc ctagagggat tgtcttatcg ctttctatcg taaccattct
     3661 ctatgccttg gtgacccttg ttttgactgg tgtggttcac tatagtcatt taaatgtcga
     3721 tgatgccgtt gcctttgccc ttcgtagtgt tgggattagt tgggcagcca actatgtgtc
     3781 attagtggct atcttgacct tgattacagt ttgtatttcg atgacctatg ccctatcgcg
     3841 tatgatttac agtttagcac gtgacggctt agtgcctgct gcctttaagg aactgacgaa
     3901 gactagcaag ataccaaaga atgctactat cttgacaggt ctagcttcag cagtagcagc
     3961 aggaatgttc ccactagcca gtattgcagc ctttttaaat atttgtacct tagcctactt
     4021 gatcatgttg gcttatggtc tgattcgctt acggaaagaa aaaggaatgc ccaaagctgg
     4081 ggaatttaaa acaccattgg tacctctgtt accaatttta tcgatcatca tttgtttatc
     4141 cttcatgtta cagtataata tgaatacctg gattgctttc ctagttgcgt tgttagtagg
     4201 aagtattatt tacttcactt atggctataa gcattctacc atagaagaat aaagtgaaag
     4261 tctattgagt tgttgacact cagcagattt ttatatgtga aagaaatttc
//
LOCUS       DAVLVP010000116         4222 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.116, whole genome shotgun sequence.
ACCESSION   DAVLVP010000116 DAVLVP010000000
VERSION     DAVLVP010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.116"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..1638
                     /locus_tag="VYN69_001866"
     CDS             <1..1638
                     /locus_tag="VYN69_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1534080.1"
                     /translation="SASASASTSASVSASTSASESASTSASASASTSASASASTSASA
                     SASTSASASASTSASASASTSASASASTSASTSASTSASASASTSASASASTSASASA
                     STSASASASTSASESASTSASASASTSASESASTSASASASTSASASASTSASASAST
                     SASASASTSASESASTSASASASTSASASASTSASASASTSASESASTSASASASTSA
                     SESASTSASASASTSASESASTSASASASTSASASASTSASASASTSASASASTSAST
                     SASTSASASASTSASASASTSASASASTSASASASTSASESASTSASASASTSASASA
                     STSASASASTSASASASTSASASASTSASTSASTSSSASASISASESASTSASASAST
                     SASASASTSASALASTSASASASTSASESASTSASASASTSASASASTSASALASTSA
                     SESASTSASASASTSASASASTSASASESASTSASASASESASTSASASTSAGKSRQQ
                     LPNTGTEASKSSVLLGALAAVTGLGLFAKRRKRDDEE"
     gene            1685..3085
                     /locus_tag="VYN69_001867"
     CDS             1685..3085
                     /locus_tag="VYN69_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000543101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 2"
                     /protein_id="HEV1534081.1"
                     /translation="MHALVDAMSERITLLANMGYPLEKHLAIYRQMLEFSLANGQASG
                     LSDTATYKEFEMKKRLLNQLTVQESSEKKAIVLAANYAYVDQVLTTIRSICYHNRSLR
                     FYLIHSDFPNEWIKQLNKRLEKFDSEIINCRVTSEQISCYKSDISYTVFLRYFIADFV
                     QEDKALYLDCDLVVTKNLDDLFATDLQDYPLAAVRDFGGRAYFGQEIFNAGVLLVNNA
                     FWKKENMTQKLIDLTNEWHDKVDQADQSILNMLFEHKWLELDFDYNHIVIHKQFADYQ
                     LPEGQDYPAIIHYLSHRKPWKDLAAQTYREVWWYYHGLEWTELGQNHHLHPLQRSHIY
                     PIKEPFTCLIYTASDHIEQIETLVQSLPDIQFKIAARVIVSDRLAQMTIYPNVTIFNG
                     IHYLVDVDNELVETSQVLLDINHGEKTEEILDQFANLGKPILSFENTKTYEVGQEAYA
                     VDQVQAMIEKLREISK"
     gene            3082..3960
                     /locus_tag="VYN69_001868"
     CDS             3082..3960
                     /locus_tag="VYN69_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis protein"
                     /protein_id="HEV1534082.1"
                     /translation="MKKNHLVGDALILTVSDQIEELDYLLESLSNICFHIAAPVQFSE
                     KIRSLETNYNVRLRTITNEEQLNFLVDTCDFLLDINHFQEVDAIVSKFVQAGKSVFAF
                     DNTVHGNQGQEVFLSSTPDKLVSRVRDYLNEVRVGTNHQEKIIQDGTWNVFKIDDKAH
                     FIVGANVACRNFENFHVSSGKLILNDGVFINNSCSFNCMERIEIGAGTMMGEGVRFYD
                     HDHIYTAEKIEKWQWTTAPIRVGRDCWIGSNVTILKGVTIGDNTIIGAGCLIRNDIPS
                     NSVVYNNGNLFVKMRV"
BASE COUNT         1253 a          928 c          897 g         1144 t
ORIGIN      
        1 agcgcctcag cctcagcatc aacaagtgct tcagtctcag cgtcaaccag tgcctctgaa
       61 tccgcatcaa ccagtgcatc ggcttcagca agtactagtg catcagcttc agcatcaacg
      121 agtgcatcgg cttcagcaag caccagtgct tcggcttcag caagcacaag cgcctcagct
      181 tcagcatcaa caagtgcatc ggcttcagca tcaacaagtg caagtacgtc agcttcaaca
      241 agtgcatcag cttcagcaag taccagtgcg tcagcctcag catcaaccag tgcttcggct
      301 tcagcaagca caagcgcctc agcctcagca agtacatcag cttctgaatc cgcatcaacg
      361 agtgcttcgg cttcagcaag cacctcagcg tctgaatcgg cctcaaccag tgcgtcagcc
      421 tcagcaagta caagtgcgtc agcttcagca agtacaagtg cgtcagcctc agcatcaacc
      481 agtgcctcgg cttcagcaag cacctcagct tctgaatcgg cctcaaccag tgcttcggct
      541 tcagcgtcaa cgagtgcttc ggcttccgca tcaaccagtg cgtcagcttc agcaagcacc
      601 tcagcgtctg aatcggcctc aacaagtgcg tcggcttcag caagtacctc agcttctgaa
      661 tcggcctcaa cgagtgcttc ggcttcagca agtacctcag cttctgaatc ggcctcaacg
      721 agtgcttcgg cttcagcaag tacaagtgcg tcagcttcag caagtacaag tgcgtcagct
      781 tcagcatcaa cgtctgcctc agcttcagcg tcaaccagcg caagtacgtc agcttcaaca
      841 agtgcatcag cttcagcaag taccagtgcg tcagcctcag caagcaccag cgcctcagct
      901 tccgcatcaa ccagtgcctc ggcttcagca agcacctcag cttctgaatc ggcctcaacc
      961 agtgcttcgg cttcagcgtc aacgagtgct tcggcttcag catcgacaag cgcctcagct
     1021 tcagcgtcaa ccagcgcctc agcttccgca tcaaccagtg cttcagcttc agcgtcaacc
     1081 agcgcaagta cgtcagcgtc aacgagttcc tcagcctcag caagtatctc agcgtctgaa
     1141 tctgcatcaa ccagtgcctc agcctcagca agtaccagcg cctcggcctc agcgtcgaca
     1201 agtgcttcag ccttagcctc aacgagtgcc tcagcctcag caagcacctc agcgtctgaa
     1261 tctgcatcga caagcgcctc ggcttcagca agcacaagcg cctcagcttc cgcatcaacg
     1321 agtgcgtcag ccttagcaag cacatcagct tctgaatctg catcaacgtc tgcctcagct
     1381 tcagcgtcaa ccagtgcgtc ggcttcagcg tcgacaagtg cgtcagcttc tgaatcggca
     1441 tcaacgagtg cctcagcttc agcgtctgaa tcggcatcaa caagcgcaag cgcgtcaact
     1501 tcagcaggta agtcaagaca gcaattgcca aatacaggta cagaagcttc caagtcatct
     1561 gtactccttg gagctttggc agctgtgact ggcttgggtt tgtttgcgaa acgccgtaaa
     1621 cgtgatgatg aagaataatc ggattagaaa aggtagttta tcaagagttt ggacagaaaa
     1681 gtggatgcat gcattggttg atgccatgtc cgaacgcatt acgctactag ctaatatggg
     1741 ttacccttta gaaaagcatt tggcgattta tcgtcaaatg ttggaattca gtctcgccaa
     1801 cggtcaagct agtggtttat ctgacacagc aacgtataaa gagtttgaaa tgaaaaaaag
     1861 acttttaaat cagttaacgg tacaagaatc aagtgaaaag aaagccattg tcctagcggc
     1921 gaattatgcc tatgtagacc aagttttaac gacaatcagg tctatctgtt accacaatcg
     1981 ctcgcttcgt ttttatttga ttcatagcga ttttccaaat gaatggatca aacaattaaa
     2041 taagcgatta gagaagtttg actcagaaat tattaattgt cgggtaactt ctgagcaaat
     2101 ttcatgttat aaatcggata ttagttacac agtcttttta cgctatttca tagctgattt
     2161 cgtgcaagaa gacaaggccc tctacttgga ctgtgatcta gttgtaacga aaaatctgga
     2221 tgacttgttt gctacagact tacaagatta tcctttggct gctgttagag attttggggg
     2281 cagagcttat tttggtcaag aaatctttaa tgccggtgtt ctcttggtaa acaatgcttt
     2341 ttggaaaaaa gagaatatga cccaaaaatt aattgattta accaatgaat ggcatgataa
     2401 ggtggatcag gcagatcaga gcatcttgaa tatgcttttt gaacataaat ggctggaatt
     2461 ggattttgat tataatcata ttgtcattca taaacagttt gctgattatc aattgcctga
     2521 gggtcaggat tatcctgcta ttattcacta cctttcccat agaaaaccgt ggaaagattt
     2581 ggcggcccaa acttatcgtg aagtttggtg gtactatcat gggcttgaat ggacagaatt
     2641 gggacaaaac catcatttac atccattaca aagatctcac atctatccaa taaaggaacc
     2701 tttcacttgt ctaatctata ctgcctcaga ccatattgaa caaattgaga cattggttca
     2761 atccttgcct gatattcagt ttaagatagc agctagagta atagttagtg atcgattggc
     2821 tcagatgaca atttatccaa acgtgactat atttaacgga attcactatt tggtagatgt
     2881 cgataatgaa ttggtagaaa ccagtcaagt acttttagat attaatcatg gcgaaaagac
     2941 agaagaaatt ctcgatcaat ttgctaatct tggcaagcct atcttatcct ttgaaaatac
     3001 taaaacctat gaagtaggtc aggaggcata tgctgttgac caagttcaag caatgattga
     3061 gaaattgaga gaaataagca aatgaagaaa aatcatttag taggagatgc tctgattttg
     3121 acggttagtg atcagattga agagttggat tatctccttg aatccttgtc aaatatctgt
     3181 tttcatattg cagcacctgt tcaattttcc gagaaaatcc gtagcttaga aacgaactat
     3241 aatgtccgtc tccgaacgat tacaaatgaa gaacagctaa actttttagt cgatacctgt
     3301 gactttttac tagatatcaa tcactttcaa gaagtagatg ctattgtctc caagtttgta
     3361 caggctggaa aatctgtctt tgcatttgac aatacagtcc atggaaatca ggggcaggaa
     3421 gtttttctat caagtacacc agacaaattg gtttctagag tgagagacta tctgaatgaa
     3481 gtcagagtag gcaccaatca tcaagagaaa atcattcaag atgggacttg gaatgtcttt
     3541 aaaatcgatg ataaggctca cttcattgta ggagccaatg tcgcctgtag aaattttgaa
     3601 aattttcatg tctcttctgg taagttaatc ctgaatgatg gtgtttttat caacaactct
     3661 tgtagtttta actgtatgga aagaattgaa atcggagctg gtaccatgat gggagagggt
     3721 gttcgttttt atgatcacga ccatatttat accgctgaaa agattgaaaa gtggcagtgg
     3781 actacagcgc cgattagagt tggcagagac tgctggatag gaagtaatgt cacgatttta
     3841 aaaggagtga cgattggaga taataccatt attggagcag gttgtctcat acgcaatgat
     3901 attccatcaa acagtgtagt ttataataat ggaaatcttt ttgtaaagat gagagtttaa
     3961 tgagtaatat aaaatcaagt tagatgaaac tttctaaggt tggaagaaaa ccaaacatga
     4021 cttcagagga tatcttaatg gtgagtcttt aatctatttg ataatgccga atctataagt
     4081 agatagttgt aaattttgca gaacacgaaa gaaatcttat tcatagcata attgatttga
     4141 agattttttg attcaacatt tagatttaga aatgaaaaga tatcatcgaa gaaaaggata
     4201 ctgtaattgt tgatgaaaca ga
//
LOCUS       DAVLVP010000117         4217 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.117, whole genome shotgun sequence.
ACCESSION   DAVLVP010000117 DAVLVP010000000
VERSION     DAVLVP010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4217)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4217)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4217
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.117"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            648..1640
                     /locus_tag="VYN69_001869"
     CDS             648..1640
                     /locus_tag="VYN69_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018371202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1534083.1"
                     /translation="MAMIEVEHLQKHFVKIVKEPGLKGALRSFIHPEKQTFEAVKDLT
                     FEVPKGQILGFIGANGAGKSTTIKMLTGILKPTSGFCRINGKIPQDNRQDYVKDIGVV
                     FGQRTQLWWDLALQETYTVLKEIYDVPDSLFHKRMDFLNEVLDLKDFIKDPVRTLSLG
                     QRMRADIAASLLHNPKVLFLDEPTIGLDVSVKDNIRRAITQINQEEETTILLTTHDLS
                     DIEQLCDRIFMIDKGQEIFDGTVSQLKETFGKMKTLSFELLPGQSHLVSHYDGLSDMT
                     IDRQGNSLNIEFDSSRYQSADIIKQTLSDFEIRDLKMVDTDIEDIIRRFYRKEL"
     gene            1642..2460
                     /locus_tag="VYN69_001870"
     CDS             1642..2460
                     /locus_tag="VYN69_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 family transporter protein"
                     /protein_id="HEV1534084.1"
                     /translation="MIKLWRRYKPFINAGVQELITYRVNFILYRIGDVMGAFVAFYLW
                     KAVFDSSQESLIQGFSMADITLYIIMSFVTNLLTRSDSSFMIGEEVKDGSIIMRLLRP
                     VHFAASYLFTELGSKWLIFISVGLPFLSVIVLMKIISGQGIVEVLGLTVLYLFSLTLA
                     YLINFFFNICFGFSAFVFKNLWGSNLLKTSIVAFMSGSLIPLAFFPKVVSDILSFLPF
                     SSLIYTPVMIIVGKYDASQILQALLLQFFWLLVMVGLSQLIWKRVQSFITIQGG"
     gene            2462..3247
                     /locus_tag="VYN69_001871"
     CDS             2462..3247
                     /locus_tag="VYN69_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003010202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEV1534085.1"
                     /translation="MKKYQRMHLIFIRQYIKQIMEYKVDFVVGVLGVFLAQGLNLLFL
                     NVIFQHIPFLEGWTFQEIAFIYGFSLIPKGMDHLFFDNLWALGQRLVRKGEFDKYLTR
                     PINPLFHILVETFQIDALGELLVGGILLGTTVTSIVWTLPKFLLFLVCIPFATLIYTS
                     LKIATASIAFWTKQSGAMIYIFYMFNDFAKYPISIYNSLLRWLISFIVPFAFTAYYPA
                     SYFLQEKDVFFNVGGLMLISLVFFVISLKLWDKGLDSYESAGS"
     gene            3286..3564
                     /locus_tag="VYN69_001872"
     CDS             3286..3564
                     /locus_tag="VYN69_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HEV1534086.1"
                     /translation="MFVIEEVKDENQKKAVVAEVLKDLPEWFGIPESTQAYIEGTTTL
                     QVWTAYQESDLTRFVSLSYSSEDCAEIDCLGVKKLIKVEKLGANCLLL"
     gene            3549..3746
                     /locus_tag="VYN69_001873"
     CDS             3549..3746
                     /locus_tag="VYN69_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1534087.1"
                     /translation="MLATLESEARKKVGYLQVKTVAEGSNKDYDRTNDFYRGLGFKKL
                     EIFSQLWNPQNPCQILIKKLE"
     gene            complement(4029..>4217)
                     /locus_tag="VYN69_001874"
     CDS             complement(4029..>4217)
                     /locus_tag="VYN69_001874"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534088.1"
                     /translation="GCSKHCFEVVDGTDEVSSKHCFEVVDRTDEVSNHTHGKATLTWF
                     EEIFEEYKSKTFVNRDTL"
BASE COUNT         1160 a          737 c          912 g         1408 t
ORIGIN      
        1 tatgaaagct ttttatatca ctattgaaaa atttatacga gatgatgaaa gccttaagtg
       61 ttattttaca aaggttattt caagtcgttc caaggtaaca agtctagatc agattgaagc
      121 tgataaaacg atacaaagaa aatattcaag tgagctaaaa aaatttattg gattttataa
      181 tgagattatt tgtgaggaaa atagtttcct acatgtacga aagaggtggt cgagttggtt
      241 taggtagtcg gtgcgtgagt tgataattct cagggtatgg acttcttttt catgaatgag
      301 gtaaaagagc aggtattgtt tagagacaat cattctgagc atattttctg gatagaggga
      361 gtatccgatt ttatgatcaa agttaatacc gccctctggt gagaagatga gtaggttggt
      421 aatttaaact attaaacaga atttttgatt aaaagtatta tttcatgaga gaaatcctaa
      481 tttcacaatt cataggtaaa cgcttgcatt ctgtttttta ttggactata ataggttggt
      541 ataaagcctt ctgtagtaat aaaatgtaga aggtgtagaa agtaaggatt tagaatattt
      601 gtagttaaaa acacaatgtt gctattcctt acgataggga gatagatatg gcaatgatag
      661 aagtggaaca tcttcagaaa cattttgtga agattgttaa ggaaccgggc ttgaaggggg
      721 ctttgcgctc ctttattcat cctgaaaagc agacctttga agcggtcaag gatttgacct
      781 ttgaggttcc aaaagggcag attttaggat ttatcggggc aaatggtgct gggaagtcga
      841 caaccattaa aatgctgaca ggaattttga aaccaacatc tggtttttgt cggattaacg
      901 gcaagattcc ccaggataat cggcaagatt atgtcaaaga tattggcgta gtctttggac
      961 aacgcaccca gctatggtgg gatttggctc tgcaagagac ctacactgtc ttaaaagaga
     1021 tttatgatgt gccagactcg ctctttcata agcgtatgga ctttttgaat gaagtcttgg
     1081 atttgaagga ctttatcaag gatcccgtgc ggactctttc actgggacaa cggatgcggg
     1141 cggatattgc ggcctccttg ctccacaatc ccaaggttct ttttttagat gagccgacca
     1201 ttggtttgga cgtttcggtt aaggataata ttcgtcgggc aattactcag atcaatcaag
     1261 aggaagaaac taccattctt ttgaccactc acgatttgag tgatattgag caactttgtg
     1321 atcggatttt catgattgac aaggggcaag agatttttga tggaacggtg agccaactca
     1381 aggagacctt tggtaagatg aagactctct cttttgaact gctaccaggt caaagtcatc
     1441 tcgtctctca ctatgacggt ctgtctgata tgaccattga tagacaagga aacagcctca
     1501 acattgaatt tgatagttct cgctaccagt cagctgacat tatcaagcaa accctgtctg
     1561 attttgaaat ccgcgatttg aagatggtgg atacggatat tgaggatatt atccgtcgct
     1621 tctaccgaaa ggagctctag gatgatcaaa ttgtggagac gttataaacc ctttatcaat
     1681 gcaggggttc aggagttgat tacttaccga gtcaacttta ttctctatcg gattggcgat
     1741 gtcatggggg cttttgtggc cttttatctc tggaaggctg tctttgattc ttcgcaagag
     1801 tctttgattc agggcttcag tatggcggat atcaccctct acatcatcat gagttttgtg
     1861 accaatcttc tgactagatc cgattcgtcc tttatgattg gggaggaggt caaggatggc
     1921 tccattatca tgcgtttgtt gcgaccagtg cattttgcgg cctcctatct tttcaccgag
     1981 cttggttcca agtggttgat ttttatcagc gttggccttc catttttaag tgtcattgtc
     2041 ttgatgaaaa tcatatcggg tcaaggtatt gtagaggtgc taggattaac tgtcctttat
     2101 ctttttagct taacgctcgc ctatctgatt aactttttct ttaatatttg ctttggattt
     2161 tcagcctttg tgtttaaaaa tctttggggt tccaacctac ttaagacttc catagtggct
     2221 tttatgtcgg ggagtttgat tcccttggca ttctttccaa aggttgtttc agatattctc
     2281 tcctttttgc ctttttcatc cttgatttat actccagtta tgatcattgt tggaaaatac
     2341 gatgccagtc agattcttca ggcactcctt ttgcagttct tctggctctt agtgatggtg
     2401 ggattgtctc agttgatttg gaaacgggtc cagtccttta tcaccattca aggaggttag
     2461 tatgaaaaaa tatcaacgaa tgcatctgat ttttatcaga caatacatca aacaaatcat
     2521 ggaatataag gtggattttg tggttggtgt cttgggagtc tttctggctc aaggcttgaa
     2581 tctcttgttt ctcaatgtca tctttcaaca tattccattc ctagaaggct ggacctttca
     2641 agagatagct ttcatttatg gattttcctt gattcccaag ggaatggacc atctcttttt
     2701 tgacaatctc tgggcactag ggcaacgcct agtccgaaaa ggagagtttg acaagtatct
     2761 gactcgtccc atcaatcctc tctttcacat cctagttgaa acctttcaga ttgatgcctt
     2821 gggtgaactc ttagtcggtg gtattttatt gggaacaaca gtgaccagca ttgtttggac
     2881 tcttccaaaa ttcctgcttt tcctagtttg tattcctttt gcgaccttga tttatacttc
     2941 tcttaaaatc gcaacagcca gtatcgcctt ttggactaag cagtcaggcg ccatgattta
     3001 catcttctat atgttcaatg actttgctaa gtatccgatt tctatttaca attctcttct
     3061 tcgttggttg attagcttta tcgtgccttt cgcctttaca gcctactatc cagctagcta
     3121 tttcttacag gaaaaggatg tgttctttaa cgtaggaggt ttgatgttga tttctctggt
     3181 tttctttgtt atttccctta aactttggga taagggctta gattcctacg aaagtgcggg
     3241 ttcgtaaaag ctaaagtaag actaaaatca agaaagaaac ttatgatgtt tgtaattgaa
     3301 gaagtcaagg atgaaaatca aaaaaaggca gttgtagctg aggttttgaa ggatttgcca
     3361 gaatggtttg gaatcccaga aagcacacaa gcctatatag aaggaaccac gacactgcaa
     3421 gtttggaccg cctatcagga gagtgatttg actagatttg taagcttatc ctattcgagt
     3481 gaagattgtg cagagattga ttgtctcggc gtaaaaaagc ttatcaaggt agaaaaattg
     3541 ggagccaatt gcttgctact ttagagagtg aagctcgtaa aaaagttggt tatctgcagg
     3601 tcaaaacagt ggcagaaggt tctaataaag attatgatcg aacaaatgac ttttatcgag
     3661 gtcttggctt taaaaagtta gagatttttt ctcagctatg gaatccgcaa aatccttgtc
     3721 agattttgat taaaaagctt gaataatatt acttgacatc tattttttat tctcagagtg
     3781 ctatactgta agtgtaatcg ccgatttagc tcagttggta gagcaaggca ctcgtaaagc
     3841 ctaggttata ggtagataaa cgactgagga gttgaaaaaa tagataggta gaagataacc
     3901 gttaagcctt actcttagcg gttatttata ttgtttaata gcgctaatat ttcatcaatt
     3961 atgcctgttt tcgtgtttct ggtagttgtt caagtttatt gctactattt ttgatggtat
     4021 gaatgtgctt ataatgtatc ccggttaacg aaagttttgg acttatactc ttcgaaaatc
     4081 tcttcaaacc acgtcaacgt cgccttgccg tgcgtatggt tactgacttc gtcagttcta
     4141 tccacaacct caaaacagtg ttttgagctg acttcgtcag ttccatctac aacctcaaaa
     4201 cagtgttttg agcaacc
//
LOCUS       DAVLVP010000118         4132 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.118, whole genome shotgun sequence.
ACCESSION   DAVLVP010000118 DAVLVP010000000
VERSION     DAVLVP010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4132
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.118"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..171)
                     /locus_tag="VYN69_001875"
     CDS             complement(<1..171)
                     /locus_tag="VYN69_001875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534089.1"
                     /translation="MADRTDEVSSKHRFEVADRTDEVSSKHRFEVADRTDEVSSKHCF
                     EVVDRTDEVSSKH"
     gene            569..796
                     /locus_tag="VYN69_001876"
     CDS             569..796
                     /locus_tag="VYN69_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000576349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534090.1"
                     /translation="MIFKAFKTKKQRKRQVELLLTIFFDSFLIDLFLHLFGIVPFKLD
                     KILIVSLIIFPIISTSIYAYEKLFEKVFDKD"
     gene            1084..3981
                     /gene="nanA"
                     /locus_tag="VYN69_001877"
     CDS             1084..3981
                     /gene="nanA"
                     /locus_tag="VYN69_001877"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014632427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored neuraminidase NanA"
                     /protein_id="HEV1534091.1"
                     /translation="MNRSVQERKCRYSIRKLSVGAVSMIVGAVVFGTSPVLAQEGASE
                     QPLANETQLSGESSTLTDTEKSQPSSETELSGNKQEQERKDKQEEKIPRDYYARDLEN
                     VETVIEKEDVETNASNGQRVDLSSELDKLKKLENATVHMEFKPDAKAPAFYNLFSVSS
                     ATKKDEYFTMAVYNNTATLEGRGSDGKQFYGNYNDAPLKVKPGQWNSVTFTVEKPTPE
                     LPKGRVRLYVNGVLSRTSLKSGNFIKDMPDVTHVQIGATKRANNTVWGSNLQIRNLTV
                     YNRALTPEEVQKRSQLFKRSDLEKKLPEGAVLTEKTDIFESGRNGKPNKDGIKSYRIP
                     ALLKTDKGTLIAGADERRLHSSDWGDIGMVIRRSEDNGKTWGDRVTITNLRDNPKASD
                     PSIGSPVNIDMVLVQDPETKRIFSIYDMFPEGKGIFGMSSQKEEAYKKIDGKTYQILY
                     REGEKGAYTIRENGTVYTPDGKATDYRVVVDPVKPAYSDKGDLYKGDQLLGNIYFTTN
                     KTSPFRIAKDSYLWMSYSDDDGKTWSAPQDITPMVKADWMKFLGVGPGTGIVLRNGPH
                     KGRILIPVYTTNNVSHLDGSQSSRVIYSDDHGKTWHAGEAVNDNRQVDGQKIHSSTMN
                     NKRAQNTESTVVQLNNGDVKLFMRGLTGDLQVATSKDGGVTWEKDIKRYPQVKDVYVQ
                     MSAIHTMHEGKEYIILSNAGGPKRENGMVHLARVEENGELTWLKHNPIQKGEFAYNSL
                     QELGNGEYGILYEHTEKGQNAYTLSFRKFNWEFLSKNLISPTEAKVKRTREMGKGEMG
                     KGVIGLEFDSEVLVNKAPTLQLANGKTATFLTQYDSKTLLFAVDKEDIGQEIIGIAKG
                     SIESMHNLPVNLAGARVPGGVNGSKAAVHEVPEFTGGVNGTEPAVHEIAEYKGSDSLV
                     TLTTKEDYTYKAPLAQQALPETGNKESDLLASLGLTAFFLGLFTLGKKREQ"
BASE COUNT         1386 a          707 c          907 g         1132 t
ORIGIN      
        1 gtgttttgag ctgacttcgt cagttctatc cacaacctca aaacagtgtt ttgagctgac
       61 ttcgtcagtt ctatctgcca cctcaaaacg gtgttttgag ctgacttcgt cagttctatc
      121 tgccacctca aaacggtgtt ttgagctgac ttcgtcagtt ctatctgcca cctcaaaacg
      181 gtgttttgag ctgactttgt cagtcttatc tacaacctca aaacagtgtt ttgagcatca
      241 tgcggctagc ttcttagttt gctctttgat tttcattgag tataaaaaca gatgagtttc
      301 tgttttcttt ttatggacta taaatgttca gctgaaacta ctttcaagga cattattata
      361 taaaagaatt ttttgaaact aaaatctact atattacact atattgaaag cgttttaaaa
      421 ataaggtata ataaatttat taacacttat aaaaagtgat agaatctatc tttatgtata
      481 tttaaagata gattgctgta aaaatagtag tagctatgcg aaataacaga tagagagaag
      541 ggattgaagc ttagaaaagg ggaataatat gatatttaag gcattcaaga caaaaaagca
      601 gagaaaaaga caagttgaac tacttttgac aatttttttc gacagttttc tgattgattt
      661 atttcttcac ttatttggga ttgtcccctt taagctggat aagattctga ttgtgagctt
      721 gattatattt cccattattt ctacaagtat ttatgcttat gaaaagctat ttgaaaaagt
      781 gttcgataag gattgagcag gaagtatggt ataaatagca taagctgatg tccatcattt
      841 gcttataaag agatatttta gtttaattgc agcggtgtcc tggtagataa actagattgg
      901 caggagtctg attggagaaa ggagagggga aaattggcac caatttgaga tagtttgttt
      961 agttcatttt tgtcatttaa atgaactgta gtaaaagaaa gttaataaaa gacaaactaa
     1021 gtgcattttc tggagtaaat gtcttatttc agaaatcggg atatagatat agagaggatc
     1081 agtatgaatc ggagtgttca agaacgtaag tgtcgttata gcattaggaa actatcggta
     1141 ggagcggttt ctatgattgt aggagcagtg gtatttggaa cgtctcctgt tttagctcaa
     1201 gaaggggcaa gtgagcaacc tctggcaaat gaaactcaac tttcggggga gagctcaacc
     1261 ctaactgata cagaaaagag ccagccttct tcagagactg aactttctgg caataagcaa
     1321 gaacaagaaa ggaaagataa gcaagaagaa aaaattccaa gagattacta tgcacgagat
     1381 ttggaaaatg tcgaaacagt gatagaaaaa gaagatgttg aaaccaatgc ttcaaatggt
     1441 cagagagttg atttatcaag tgaactagat aaactaaaga aacttgaaaa cgcaacagtt
     1501 cacatggagt ttaagccaga tgccaaggcc ccagcattct ataatctctt ttctgtgtca
     1561 agtgctacta aaaaagatga gtacttcact atggcagttt acaataatac tgctactcta
     1621 gaggggcgtg gttcggatgg gaaacagttt tacggtaatt acaacgatgc acccttaaaa
     1681 gttaaaccag gtcagtggaa ttctgtgact ttcacagttg aaaaaccgac accagaacta
     1741 cctaaaggcc gagtgcgcct ctacgtaaac ggggtattat ctcgaacaag tctgaaatct
     1801 ggcaatttca ttaaagatat gccagatgta acgcatgtac aaatcggagc aaccaagcgt
     1861 gccaacaata cggtttgggg gtcaaatcta cagattcgga atctcactgt gtataatcgt
     1921 gctttaacac cagaagaggt acaaaaacgt agtcaacttt ttaaacgctc agatttagaa
     1981 aaaaaactac ctgaaggagc ggttttaaca gagaaaacgg acatattcga aagcgggcgt
     2041 aacggtaaac caaataaaga tggaatcaag agttatcgta ttccagcact tctcaagaca
     2101 gataaaggaa ctttgatcgc aggtgcagat gaacgccgtc tccattcgag tgactggggt
     2161 gatatcggta tggtcatcag acgtagtgaa gataatggta aaacttgggg tgaccgagta
     2221 accattacca acttacgtga caatccaaaa gcttctgacc catcgatcgg ttcaccagtg
     2281 aatatcgata tggtgttggt tcaagatcct gaaaccaaac gaatcttttc tatctatgac
     2341 atgttcccag aagggaaggg aatctttgga atgtcttcac aaaaagaaga agcctacaaa
     2401 aaaatcgatg gaaaaaccta tcaaatcctc tatcgtgaag gagaaaaggg agcttatacc
     2461 attcgagaaa atggtactgt ctatacacca gatggtaagg cgacagacta tcgcgttgtt
     2521 gtagatcctg ttaaaccagc ctatagcgac aagggtgatc tatacaaggg tgaccaatta
     2581 ctaggcaata tctacttcac aacaaacaaa acttctccat ttagaattgc caaggatagc
     2641 tatctatgga tgtcctacag tgatgacgac gggaagacat ggtcagcgcc tcaagatatt
     2701 actccgatgg tcaaagccga ttggatgaaa ttcttgggtg taggtcctgg aacaggaatt
     2761 gtacttcgga atgggcctca caagggacgg attttgatac cggtttatac gactaataat
     2821 gtatctcact tagatggctc gcaatcttct cgtgtcatct attcagatga tcatggaaaa
     2881 acttggcatg ctggagaagc ggtcaacgat aaccgtcagg tagacggtca aaagatccac
     2941 tcttctacga tgaacaataa acgtgcgcaa aatacagaat caacggtggt acaactaaac
     3001 aatggagatg ttaaactctt tatgcgtggt ttgactggag atcttcaggt tgctacaagt
     3061 aaagacggag gagtgacttg ggagaaggat atcaaacgtt atccacaggt taaagatgtc
     3121 tatgttcaaa tgtctgctat ccatacgatg cacgaaggaa aagaatacat catcctcagt
     3181 aatgcaggtg gaccgaaacg tgaaaatggg atggtccact tggcacgtgt cgaagaaaat
     3241 ggtgagttga cttggctcaa acacaatcca attcaaaaag gagagtttgc ctataattcg
     3301 ctccaagaat taggaaatgg ggagtatggc atcttgtatg aacatactga aaaaggacaa
     3361 aatgcctata ccctatcatt tagaaaattt aattgggaat ttttgagcaa aaatctgatt
     3421 tctcctaccg aagcgaaagt gaagcgaact agagagatgg gcaaaggaga gatgggcaaa
     3481 ggagttattg gcttggagtt cgactcagaa gtattggtca acaaggctcc aacccttcaa
     3541 ttggcaaatg gtaaaacagc gactttccta acccagtatg atagcaagac cttgttgttt
     3601 gcagtagata aggaagatat cggacaggaa attattggta tagctaaagg aagcatcgaa
     3661 agtatgcata atcttcctgt aaatctagca ggtgccagag ttcctggcgg agtaaatggt
     3721 agcaaagcag cggtgcatga agttccagaa tttacagggg gagttaatgg tacagagcca
     3781 gctgttcatg aaatcgcaga gtataaggga tctgattcgc ttgtaactct tactacaaaa
     3841 gaagattata cttacaaagc tcctcttgct cagcaggcac ttcctgaaac aggaaacaag
     3901 gagagtgacc tcctagcttc actaggacta acagctttct tccttggtct gtttacgcta
     3961 gggaaaaaga gagaacaata agagaagaat tctaaacatt tgattttgta aaaatagaag
     4021 gagatagcag gttttcaagc ctgctatctt tttttgatga cattcaagct gatacgaaat
     4081 cataagaggt ctgaaactac tttcagagta gtctgttcta taaaatatag ta
//
LOCUS       DAVLVP010000119         4130 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.119, whole genome shotgun sequence.
ACCESSION   DAVLVP010000119 DAVLVP010000000
VERSION     DAVLVP010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4130
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.119"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..1072
                     /gene="dnaN"
                     /locus_tag="VYN69_001878"
     CDS             <1..1072
                     /gene="dnaN"
                     /locus_tag="VYN69_001878"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEV1534092.1"
                     /translation="SSKNAIPILSTIKIDVTNEGITLIGSNGQISIENFISQKNEDAG
                     LLITSLGSILLEASFFINVVSSLPDVTLDFKEIEQNQIVLTSGKSEITLKGKDSEQYP
                     RIQEISASTPLILETKLLKKIINETAFAASTQESRPILTGVHFVLSQHKELKTVATDS
                     HRLSQKKLTLEKNSDDFDVVIPSRSLREFSAVFTDDIETVEIFFANNQILFRSENISF
                     YTRLLEGNYPDTDRLIPTDFNTTITFNVVNLRQSMERARLLSSATQNGTVKLEIKDGV
                     VSAHVHSPEVGKVNEEIDTDQVTGEDLTISFNPTYLIDSLKALNSEKVTISFISAVRP
                     FTLVPADTDEDFMQLITPVRTN"
     gene            1137..1331
                     /locus_tag="VYN69_001879"
     CDS             1137..1331
                     /locus_tag="VYN69_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 family protein"
                     /protein_id="HEV1534093.1"
                     /translation="MYQVGNFVEMKKPHACTIKSTGKKANRWEITRVGADIKIKCSNC
                     EHVVMMGRYDFERKMNKIID"
     gene            1415..2530
                     /gene="ychF"
                     /locus_tag="VYN69_001880"
     CDS             1415..2530
                     /gene="ychF"
                     /locus_tag="VYN69_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002269933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEV1534094.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDERLQKLTEMITPKKTVPTTFEFTDIAGIVKGASKGEGLGNKFLANIREVDAIVH
                     VVRAFDDENVMREQGREDAFVDPLADIDTINLELILADLESVNKRYARVEKMARTQKD
                     KESVAEFNVLQKIKPVLEDGKSARTIEFTDEEQKVVKGLFLLTTKPVLYVANVDEDVV
                     SEPDSIDYVKQIREFAATENAEVVVISARAEEEISELDDEDKKEFLEAIGLTESGVDK
                     LTRAAYHLLGLGTYFTAGEKEVRAWTFKRGMKAPQAAGIIHSDFEKGFIRAVTMSYED
                     LVKYGSEKAVKEAGRLREEGKEYIVQDGDIMEFRFNV"
     gene            2601..3170
                     /locus_tag="VYN69_001881"
     CDS             2601..3170
                     /locus_tag="VYN69_001881"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEV1534095.1"
                     /translation="MTKLLVGLGNPGDKYFETKHNVGFMLIDQLAKKQNVTFTHDKIF
                     QADLASFFLNGEKIYLVKPTTFMNESGKAVHALLTYYGLDIDDLLIIYDDLDMEVGKI
                     RLRAKGSAGGHNGIKSIIQHIGTHVFNRVKIGIGRPKNGMSVVHHVLSKFDRDDYIGI
                     LQSVDKVDDSVNYYLQEKNFEKTMQRYNG"
     gene            3171..>4130
                     /locus_tag="VYN69_001882"
     CDS             3171..>4130
                     /locus_tag="VYN69_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000258090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEV1534096.1"
                     /translation="MVTLLDLFSENDQIKKWHQSLTDKKRQLILGLSTSTKALAIASS
                     LEKEDRIVLLTSTYGEAEGLVSDLISILGEELVYPFLVDDAPMVEFLMSSQEKIISRV
                     EALRFLTDSSKKGILVCNIAASRLILPSPNAFKDSIVKISVGEEYDQHAFIHQLKENG
                     YRKVTQVQTQGEFSLRGDILDIFEMSQLEPCRIEFFGDEIDGIRSFEVETQLSKENKI
                     ELTIFPASDMLLREKDYQRGQSALEKQISKTLSPILKSYLEEILSSFHQKQSHADSRK
                     FLSLCYDKTWTVFDYIEKDTPIFFDDYQKLMNQYEVFERELAQY"
BASE COUNT         1407 a          646 c          831 g         1246 t
ORIGIN      
        1 tagttctaaa aatgccattc ctattttatc aacaataaaa attgacgtga ccaatgaagg
       61 tattacttta attggttcaa atggtcaaat ttcaattgaa aattttattt ctcaaaaaaa
      121 tgaagatgct ggtttgttaa ttacttcttt aggttcgatc cttcttgaag cttctttctt
      181 tatcaatgta gtatctagtt tacctgatgt aactcttgat tttaaagaaa ttgaacaaaa
      241 tcaaattgtt ttaaccagtg gcaaatcaga aattacccta aaaggaaaag atagcgaaca
      301 atatccacga atccaagaaa tttcagcaag cactccttta atacttgaaa caaaattact
      361 caagaaaatt attaatgaaa cagcctttgc tgcaagtaca caagagagtc gtccgatttt
      421 aacaggtgtc cacttcgtat tgagtcaaca caaagagtta aaaacagttg caacagactc
      481 tcatcgccta agccagaaaa aattgactct tgaaaaaaat agtgatgatt ttgatgtcgt
      541 aattcctagc cgttctctac gcgaattttc agcggtattt acagatgata tcgaaactgt
      601 agagattttc tttgccaata accaaatcct ctttagaagc gaaaatatta gcttctatac
      661 tcgtctccta gaaggaaact atcctgatac agatcgcttg attccaacag actttaacac
      721 tactattact tttaatgtgg taaacttacg ccagtcaatg gagcgtgccc gtcttttatc
      781 aagtgcgact caaaatggta ctgtgaaact tgaaattaag gatggggttg ttagcgccca
      841 tgttcactct ccagaagttg gtaaagtaaa cgaagaaatc gatactgatc aggttactgg
      901 tgaagatttg accattagtt tcaacccaac ttacttgatt gattctctta aagctttaaa
      961 tagcgaaaag gtgaccatta gctttatctc agctgttcgt ccatttactc ttgtgccagc
     1021 agatactgac gaagacttca tgcagctcat tacaccagtt cgtacaaatt aagtgaaaga
     1081 ggttgagcct ggctcgcctc ttttatgata taatcgaaaa agaaaaggag agtagtatgt
     1141 atcaagttgg aaattttgtt gagatgaaaa aaccacacgc ttgtacaatc aagtcgactg
     1201 gtaaaaaggc taatcgttgg gaaattacac gtgtaggagc agatatcaaa ataaaatgca
     1261 gtaattgtga gcatgttgtc atgatggggc gatatgattt tgagcgaaaa atgaataaaa
     1321 ttattgactg agaaccctta gttagagggt tagcacttta tccctttttg tgttataata
     1381 ttagggattg aaatgaaaac ggagaatgag aaatatggct ttgacagcag gtatcgttgg
     1441 tttgccaaac gttggtaaat caacactatt taatgcaatt acaaaagcag gagcagaggc
     1501 agcaaactac ccatttgcga ctattgatcc aaatgttgga atggtggaag ttccagatga
     1561 acgcctacaa aaactaactg aaatgataac tcctaaaaag acagttccca caacatttga
     1621 atttacagat attgcaggga ttgtaaaagg agcttcaaaa ggagaagggc tagggaataa
     1681 attcttggcc aatattcgtg aagtagatgc gattgttcac gtagttcgtg cttttgatga
     1741 tgaaaatgta atgcgcgagc aaggacgtga agacgccttt gtagatccac ttgcagatat
     1801 tgatacaatt aatctggaat tgattcttgc tgacttagaa tcagtgaaca aacgatatgc
     1861 gcgtgtagaa aagatggcac gtacgcaaaa agataaagaa tcagtagcag aattcaatgt
     1921 tcttcaaaag attaaaccag tcctagaaga cgggaaatca gctcgtacca ttgaatttac
     1981 agatgaggaa caaaaggttg tcaaaggtct tttccttttg acgactaaac cagttcttta
     2041 tgtagctaat gtggacgagg atgtggtttc agaacctgac tctatcgact atgtcaaaca
     2101 aattcgtgaa tttgcagcga cagaaaatgc tgaagtagtc gttatttctg cgcgtgctga
     2161 ggaagaaatt tctgaattgg atgatgaaga taaaaaagag tttcttgaag ccattggttt
     2221 gacagaatca ggtgtagata agttgacgcg tgcagcttac cacttgcttg gattgggaac
     2281 ttacttcaca gctggtgaaa aagaagttcg cgcttggact ttcaaacgtg gtatgaaggc
     2341 tcctcaagca gctggtatta tccactcaga ctttgaaaaa ggctttattc gtgcagtaac
     2401 catgtcatat gaagatctag tgaaatacgg atctgaaaag gccgtaaaag aagctggacg
     2461 cttgcgtgaa gaaggaaaag aatatatcgt tcaagatggc gatatcatgg aattccgctt
     2521 taatgtctaa aaattaataa atggtgtcaa ttaggttgga aaaaaattcc aacccttttg
     2581 gcttttgaaa ggaaaaataa atgaccaaat tacttgtagg cttgggaaat ccaggggata
     2641 aatattttga aacaaaacac aatgttggtt ttatgttgat tgatcaacta gcgaagaaac
     2701 agaatgtcac ttttacacac gataagatat ttcaagctga cctagcatcc tttttcctaa
     2761 atggagaaaa aatttatctg gttaaaccaa cgacctttat gaatgaaagt ggaaaagcag
     2821 ttcatgcttt attaacttac tatggtttgg atattgacga tttacttatc atttacgatg
     2881 atcttgacat ggaagttggg aaaattcgtt taagagcaaa aggctcagca ggtggtcata
     2941 atggtatcaa gtctattatt caacatatag gaactcatgt ctttaaccgt gttaagattg
     3001 gaattggaag acctaaaaat ggtatgtcag ttgttcatca tgttttgagt aagtttgaca
     3061 gggatgatta tatcggtatt ttacagtctg ttgacaaagt tgacgattct gtaaactact
     3121 atttacaaga gaaaaatttt gagaaaacaa tgcagaggta taacggataa atggtgacct
     3181 tattagattt attctcagaa aatgatcaga ttaaaaaatg gcatcaaagt ttaacagata
     3241 agaaaagaca actaatactt ggtttatcaa catctactaa ggctcttgca attgcaagca
     3301 gtttagaaaa agaagatagg attgtgttat tgacgtcaac ttatggagaa gcagaaggac
     3361 ttgttagtga tcttatctct atcttgggtg aggaactcgt ctatccattt ttggtagatg
     3421 atgctcctat ggtggagttt ttgatgtctt cacaggaaaa aattatttca cgggttgaag
     3481 ccttgcgttt tttgactgat tcatctaaga aagggatttt agtttgtaat atcgcagcaa
     3541 gtcgattgat tttaccgtct cccaatgcat tcaaagatag tattgtaaaa atctcagttg
     3601 gtgaagaata tgatcaacac gcgtttatcc atcagttaaa ggaaaatggc tatcgaaaag
     3661 ttactcaagt acaaactcag ggcgaattta gtcttcgagg agatatttta gatatttttg
     3721 aaatgtccca gttagaacct tgtcgaattg agttttttgg tgatgaaatt gatggtatca
     3781 ggtcatttga agtagaaaca caattatcga aagaaaataa gatagaactc actatctttc
     3841 cagctagtga tatgcttttg agagaaaagg attatcaacg aggacagtca gctttagaaa
     3901 aacaaatttc aaaaacttta tcacctattt tgaaatcata cctagaagaa attctttcaa
     3961 gttttcatca aaagcaaagt catgcagatt ctcggaagtt tttatctttg tgctatgata
     4021 agacatggac tgtctttgat tatattgaaa aagatactcc aatattcttt gatgattatc
     4081 aaaaattgat gaatcagtat gaagtctttg aaagagaatt agcgcagtac
//
LOCUS       DAVLVP010000120         4096 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.120, whole genome shotgun sequence.
ACCESSION   DAVLVP010000120 DAVLVP010000000
VERSION     DAVLVP010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4096
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.120"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            325..1002
                     /locus_tag="VYN69_001883"
     CDS             325..1002
                     /locus_tag="VYN69_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000738309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1534097.1"
                     /translation="MKKMKEVKFHLATGLLILTYYLIFNVTSDLDFMAALSDNMYYVF
                     QVLLVLILGTIATIAFVKSEHWKECGRFQFRWSYLGVFLLSFFLLFVWANLTTYIFPR
                     TQNGSTVVEVATNLTGISYFVTRILYTSIIAPVSEEVVCRGLLMTSLSKVKRYYLDVL
                     VSAAIFGAMHVLQYGWITTDFIKYFGMGLIFCMMFRYTRSIYWAIALHASWNSFLLIV
                     TLLVFGY"
     gene            1014..1679
                     /locus_tag="VYN69_001884"
     CDS             1014..1679
                     /locus_tag="VYN69_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000720786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1534098.1"
                     /translation="MKKIISHRYFIIVFLLVIADQKFSVLVLRSDLVTGLSDFAYYLS
                     DMMLNFLVVLFALIAMIWSGKWQKINSRKFKGSYLFYSFLALLAFVVWNFVTFFLFPP
                     TRNEISYQHAAPTFTGATAFLMYFFYPVIAGPIFEEIIHRGLVMTALEKGKKWGLDVL
                     GSAVLFGVSHISNHGWVLTDFVFYMGGGLIFAVLFRMTKSIYWPIGLHIVYNGIGQLL
                     MLL"
     gene            1751..2977
                     /locus_tag="VYN69_001885"
     CDS             1751..2977
                     /locus_tag="VYN69_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000862399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1534099.1"
                     /translation="MKVFLQNRDFRQLTINQWISTLGDTIFYLAFLNYVADASFAPLA
                     ILLITISETLPQVLQIFLGVLADFQHHRVLKYTVISFAKFLLYSIVSLSLSGQSFSLL
                     LVAFICLINLLSDTLSYFSGAMLTPIFIRIIGQDHLAEAIGFKQSTVSLVKTISNILG
                     GVLLGILSIQFISLLNALTFLIAFLGILFIKTDLLKVEKTISYQEGLSVKSFCQHLLQ
                     SSKLIWNMNKVLLVLFIISTSQAVINVTVPISTLFLRNQPFLNLQTGQSLALLATLEL
                     SALILGSLMSGYLQHTISIKIALYASLLLQLLLLVGFATAHFGLILIFSFLDAFFAGI
                     LSPRLQELVFKQIPEESMGAVQSSIGAITVVLPSLFTIALVTIATSFGTLAVSFVLLL
                     FLLVAFVMLLNIRESI"
     gene            complement(3328..3405)
                     /locus_tag="VYN69_001886"
                     /pseudo
     CDS             complement(3328..3405)
                     /locus_tag="VYN69_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002674100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-galactopuranose mutase"
     gene            3476..4090
                     /locus_tag="VYN69_001887"
     CDS             3476..4090
                     /locus_tag="VYN69_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEV1534100.1"
                     /translation="MPTALGYVSIGLACGIIGVPYVTLVEMGLMSLFVYAGSAQFAML
                     ALIVVQAPVTAIAMTVFLINLRLFLLSLHASTYFRHTSLWYNIGMSSILTDETYGVLM
                     GELAHTDKVNPMWMHGNNLNSYVAWFVGTVVGTALGGLLPNPEIFALILSSVVTGKVG
                     SLPQIKWLDFLAVFPTAWVAFRYRNLVGTVLFGVVLIAVLRLVF"
BASE COUNT         1032 a          746 c          812 g         1506 t
ORIGIN      
        1 agtgtatttg tcagtgcatt aaatcctaaa ataggagcta tcatatttaa taatcttgat
       61 gaaacgaaac tagctacaat atttggagga atggttactt acttccagtt gggagatgtc
      121 gtatcaagat tattgttttc tacattggta atctatttat cttatactta tatagcagtg
      181 atatatatga tattagtttt aatagtggct atctatactt ttagaagagt acagactaca
      241 agctgagaga attttgaaat tttaacttta ttcctccagt ccttgcaatt agatataaaa
      301 taggctctta taggagataa taggatgaag aaaatgaaag aagtgaaatt tcacctagca
      361 actggcttgt tgattttgac ttattacctc atttttaatg tcacatcaga cttagatttt
      421 atggctgctt tgtctgataa tatgtactat gtgttccaag ttttattggt acttattctg
      481 ggaactatag ctacaattgc ttttgtcaaa agtgaacatt ggaaagagtg tggtcgtttc
      541 cagtttcgtt ggtcttacct gggtgttttc cttctttctt ttttcttatt gttcgtgtgg
      601 gcaaatctaa caacctatat ctttcctcgt acccagaatg gaagtacagt cgtagaagta
      661 gccaccaatt taacaggaat tagctatttt gttacgcgaa tactttatac tagtattatc
      721 gcacctgtat cagaagaggt tgtttgtcgt ggtcttttga tgacaagttt atctaaagtt
      781 aagagatact atcttgatgt tcttgtttcc gctgccatat ttggcgctat gcatgttttg
      841 cagtatggtt ggataacgac agatttcata aagtattttg gaatgggctt gatattttgt
      901 atgatgtttc gctacacccg atccatctat tgggctatag ctctgcatgc ttcatggaat
      961 agtttcttac tcattgttac tttattggta tttggatatt agaaaggtga tatatgaaaa
     1021 agataatctc acaccgctac tttattatag tttttctact agttattgct gaccagaagt
     1081 tcagtgttct agttttacgc agcgaccttg ttactgggct atctgacttt gcctattatc
     1141 tgtcggatat gatgttgaat tttcttgtgg ttttatttgc tcttattgct atgatttggt
     1201 cggggaaatg gcagaaaatc aatagtagaa agtttaaagg atcctatctt ttctattcct
     1261 tcctagctct gcttgctttt gttgtttgga atttcgttac cttttttctt ttcccaccta
     1321 ctcgaaatga aatttcttat caacatgctg ctccgacttt tacaggggct acagcatttt
     1381 tgatgtattt tttctatcct gtgattgcag gtcccatttt tgaagagata atccatcggg
     1441 gattggtgat gacagctctg gaaaaaggaa agaaatgggg gctagatgta cttggttccg
     1501 ctgttttatt tggggtctcg cacattagta atcacggttg ggtcttgaca gactttgtct
     1561 tctatatggg tggaggtctt atatttgcag tcttatttag aatgacaaag tcaatttatt
     1621 ggcctattgg actgcatata gtctacaatg gcattggtca gcttttgatg ttactgtaat
     1681 tttggttgtt agtagtatcg cggtctatcc ttggggaaag cttcccattt gaatttaaga
     1741 aggagttgtg atgaaagtat ttcttcaaaa tagagatttt agacaattaa ccatcaacca
     1801 gtggatttca acgcttgggg atacgatttt ttatctggcc ttcctgaatt atgtggcaga
     1861 tgcatctttt gcccctttgg cgattttact catcacgatt tcagaaaccc tgccccaagt
     1921 tctacaaatc tttctgggag ttttggcgga ttttcaacat catcgtgtct tgaaatacac
     1981 agtcattagt tttgcaaaat ttttgcttta ctctatagtg tctttatcac tttcagggca
     2041 gtctttctcc ttgttgctag tagcatttat ttgtctgatt aacctcttgt ctgacacatt
     2101 gagttatttt tcaggcgcca tgctcactcc gattttcatt agaattattg ggcaagacca
     2161 tctggcagaa gctattggct ttaaacagtc aactgttagt ttagtgaaaa caatcagtaa
     2221 tatcctagga ggagtcttac taggcattct atccatccag tttatttcct tactgaacgc
     2281 tcttaccttt ttaatcgcat ttttaggtat cctcttcata aaaactgacc tcttgaaagt
     2341 agaaaaaacg attagctatc aagaaggact ctctgtaaaa tccttttgcc agcatttgct
     2401 ccaatcatcg aaattgatat ggaatatgaa taaggtgctc ttggttttgt ttattatctc
     2461 tactagtcaa gcagtgataa atgttacggt tcctatttcc actctatttt taaggaacca
     2521 gccctttttg aatttacaaa caggtcaatc tcttgccttg ttagccacac tagaattgtc
     2581 agctcttatt ttgggaagtc tgatgagtgg ctatctgcaa cataccattt ccataaaaat
     2641 agccctgtat gcttcactcc tactccaatt actactccta gtgggctttg cgacagctca
     2701 ttttggcttg attctgatct ttagcttttt ggatgccttt ttcgcgggta ttctctctcc
     2761 tagattgcaa gaactcgtct ttaaacaaat acctgaggag tcaatgggag cagttcaatc
     2821 ttctatcggt gccattacgg ttgttttacc tagcttattt acaatagctt tggtaaccat
     2881 tgctactagc tttggaactc tggcagttag ctttgtttta ttgctatttc ttctagttgc
     2941 ctttgtcatg cttctgaata ttcgtgaaag tatttagtgg agaatttcta taatatatgt
     3001 ttgagactgg attgccagtc tttttatgat ggtcaaaatc aacagacttt ttctcttttc
     3061 tgaatgtgtt atagtagact gaatctaaaa tagtacgaaa caattgctaa aacatttata
     3121 gaaattaatt ttactttccc aattgatttg ttctcatctt atttcaatct gctatataat
     3181 agtccatgct gtggctggat tcttttcagc ctacttatac tcttcgaaac tctcttcaaa
     3241 ccacgtcagc ttcaccttgc cgtaggtatg gttactgact tcgtcagttc tatccacaac
     3301 ctcaaaacag tgttttgagc tgacttcatc agttctatct gcaacctcaa aacggtgttt
     3361 tgagcaacct gcggctagtt tcctagtttg ctctttgatt ttcattgagt attattaaga
     3421 caatatgagg agaaagatga aagaaaaagg attttaggag ggggcgcagg cagccatgcc
     3481 aacggctctt ggctatgtca gtatcggcct ggcctgtgga attatcggtg tgccctatgt
     3541 gacacttgtt gagatgggtt tgatgagtct ctttgtttat gctgggagtg cccagtttgc
     3601 catgttggca ctgattgtgg ttcaagctcc tgtgacagct attgctatga cggtttttct
     3661 aatcaacttg cgtctctttt tgttgagttt acacgcatcg acttatttcc gtcataccag
     3721 tctttggtac aatatcggta tgtctagtat cttgacagat gagacctatg gcgttttgat
     3781 gggcgaatta gcccatacag acaaggtaaa tcctatgtgg atgcacggaa acaatcttaa
     3841 cagctatgtg gcttggtttg tggggacagt agtcggaacg gctctgggtg gcctgctacc
     3901 aaatccagaa atctttgcct tgattctttc aagcgtagtg acaggtaagg ttgggagcct
     3961 tcctcaaatt aaatggctag acttcttagc cgtctttcca acagcttggg tagcctttcg
     4021 ctaccgcaat ctagtcggaa cagttctctt tggagtagtc ttgattgccg tcttgcgttt
     4081 ggtcttttaa atcagc
//
LOCUS       DAVLVP010000121         4076 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.121, whole genome shotgun sequence.
ACCESSION   DAVLVP010000121 DAVLVP010000000
VERSION     DAVLVP010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4076)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4076)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4076
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.121"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            18..971
                     /locus_tag="VYN69_001888"
     CDS             18..971
                     /locus_tag="VYN69_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000792120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEV1534101.1"
                     /translation="MKNKRIFKDFQASKMSLNIYTSPLLAFVFVFIGEFVAFTLYGIG
                     LLALIGLARNFGEAGQNLASYLQTLHQSLTDKTSDFRLILGLLAFGFILNTVFRWTRK
                     VEKRPIRTLGFYRENFLSNLLKGFSLGLALFLLTLLGLVVLGQYRLESIHLNPYSLAF
                     VVFTIPFWILQGTAEEVVARAWLLPQLASRTNLKLAILISSLFFTLLHMGNSGLTPLS
                     LVNLFLFGVAMALYLLKTDTVWGVAGIHGAWNFAQGNLFGILVSGQPSGTSLMTFLPQ
                     GNQDWLSGGSFGIEGSIMTSLVLLLLIVYLANKLKKENERM"
     gene            1048..3036
                     /gene="uvrB"
                     /locus_tag="VYN69_001889"
     CDS             1048..3036
                     /gene="uvrB"
                     /locus_tag="VYN69_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HEV1534102.1"
                     /translation="MINHITDNQFKLVSKYQPSGDQPQAIEQLVDNIEGGEKAQILMG
                     ATGTGKTYTMSQVISKVNKPTLVIAHNKTLAGQLYGEFKEFFPENAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSALLERNDVIVVASVSCIYGLGSPKEYA
                     DSVVSLRPGLEISRDKLLNDLVDIQFERNDIDFQRGRFRVRGDVVEIFPASRDEHAFR
                     VEFFGDEIDRIREVEALTGQVLGEVDHLAIFPATHFVTNDDHMEVAIAKIQAELEEQL
                     AVFEKEGKLLEAQRLKQRTEYDIEMLREMGYTNGVENYSRHMDGRSEGEPPYTLLDFF
                     PDDFLIMIDESHMTIGQIKGMYNGDRSRKEMLVNYGFRLPSALDNRPLRREEFESHVH
                     QIVYVSATPGDYENEQTETVIEQIIRPTGLLDPEVEVRPTMGQIDDLLGEINARVEKN
                     ERTFITTLTKKMAEDLTDYFKEMGIKVKYMHSDIKTLERTEIIRDLRLGVFDVLVGIN
                     LLREGIDVPEVSLVAILDADKEGFLRNERGLIQTIGRAARNSEGHVIMYADTVTQSMQ
                     RAIDETARRRKIQMAYNEEHGIVPQTIKKEIRDLIAVTKAVAKEEDKEVDINSLNKQE
                     RKELVKKLEKQMQEAVEVLDFELAAQIRDMMLEVKALD"
     gene            3046..3609
                     /locus_tag="VYN69_001890"
     CDS             3046..3609
                     /locus_tag="VYN69_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1534103.1"
                     /translation="MIYLRKLKREDLMSLWEMAYSQLNPVWKQYDAPYYDDYQYFSNF
                     KEFELQKSESILSNSNRLGIFVDDKLVGTVSRYWVCKQTRWMELGIGIYDKKFWNTGI
                     GKVAMLQWIDRTFQDYLELEHLGLTTWSGNIGMMKLAEKLRMKKEAHIPKVRYYQGKY
                     FDSIKYGILREDWEKINDGYYQINGNS"
     gene            3575..4063
                     /locus_tag="VYN69_001891"
     CDS             3575..4063
                     /locus_tag="VYN69_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004254187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1534104.1"
                     /translation="MTVIIKSMETPKEIEGKSLVHWQTWRESYDDLLPAEFQDTMTLE
                     RCRFFSQKYPENTLIAMDGVKVVGFISYGNFRDETIQAGEIIALYVLKDYYGKGIAQK
                     LMKAALTALNHFSEIFLWVLKDNKRAIAFYQKMGFTFDGQEKILELGKPIKEKRMVFY
                     SK"
BASE COUNT         1199 a          716 c          942 g         1219 t
ORIGIN      
        1 gaaaagagga cggggatatg aagaacaaaa gaatatttaa agacttccaa gcttcaaaaa
       61 tgagtttaaa catttacaca agccccttgt tagcctttgt ttttgtcttc ataggagagt
      121 ttgtggcttt tactttgtat ggtattggct tgttagctct catcggactt gctagaaatt
      181 ttggagaggc tggtcaaaat cttgcaagct acttgcagac cttgcatcag agcttgacgg
      241 ataaaacaag tgactttcgt ttaattttag gattactggc ctttggtttt attcttaaca
      301 ctgtgttcag atggacaaga aaagttgaga aaagacctat tcgaaccttg ggattttata
      361 gagagaattt cctcagcaat cttctgaaag gatttagtct aggcctggca ctttttcttc
      421 tgaccttgtt aggtttagtg gtcttaggtc aatatcgttt ggaatccatt cacttgaatc
      481 cttattctct tgcctttgtc gtctttacta tcccattttg gattttacag gggacagcag
      541 aagaagtggt ggcccgtgct tggctacttc ctcaattggc ctcaagaacc aatctaaaac
      601 tagctattct tatatctagc ctgttcttta ccctgcttca tatgggcaat tctggtctca
      661 cccctctatc tctagtaaat ctctttttat tcggagttgc catggctctt taccttctca
      721 aaactgatac agtttggggt gttgcaggta ttcatggtgc ttggaatttt gctcagggta
      781 atctctttgg gattttagtt agtggtcaac cgtcaggaac gtctctgatg acctttttac
      841 cacaaggcaa tcaagattgg ctatcaggtg gttcttttgg catagaaggt tccattatga
      901 caagtctggt attactactg ctgattgtct atcttgctaa taaattaaag aaagaaaatg
      961 aaaggatgtg acttcggtcc gtccttttct tcgtgaaaat actataagta tgctaaaata
     1021 ggaatagcac atggagagag gattcttatg atcaatcaca ttacagataa tcaatttaaa
     1081 ctagtatcaa aatatcaacc atcaggagat caaccccaag ctatcgagca gttggtggat
     1141 aacattgagg ggggagaaaa agctcagatt ctgatggggg cgactggaac agggaagacc
     1201 tatactatga gtcaggtcat ttctaaagtc aataaaccaa ctctggttat tgcccacaat
     1261 aaaactctgg ctggtcagct ctatggggag tttaaggaat ttttccctga aaatgcagtt
     1321 gagtatttcg tatcctacta tgattattac cagccagagg cctatgtccc ttctagcgat
     1381 acctatattg agaaggatag ttctgtcaat gacgagattg acaagcttcg ccactcagct
     1441 acctcagccc ttttggagcg taatgatgtt attgtcgtgg cctcagtctc ttgtatctat
     1501 ggtttgggtt cgcccaagga atacgctgat agtgtcgtta gtctccgtcc tggtctagag
     1561 atttctcgtg ataaactctt gaatgacttg gtcgatattc agtttgaacg taatgatatt
     1621 gatttccaac gcggaagatt tcgcgttcgt ggggatgtgg tagagatttt cccagcttcc
     1681 cgagatgaac atgcctttcg agtagaattt tttggagacg aaattgaccg tattcgtgaa
     1741 gttgaggctc tgacaggtca ggtgttggga gaagtggatc atttagcgat tttcccagcg
     1801 acacactttg tgaccaatga cgaccacatg gaagttgcca ttgcaaagat tcaggccgag
     1861 ttggaagaac aattagctgt ctttgaaaag gaaggtaaac tgcttgaagc ccagcgtttg
     1921 aaacagcgga cagagtatga tatcgaaatg ttgcgtgaga tgggctatac caatggggtt
     1981 gaaaattatt ctcgccacat ggatggacgg agcgaaggag agcctcctta tacgcttctc
     2041 gacttcttcc cagatgattt cttgattatg attgacgaga gtcatatgac catagggcaa
     2101 atcaagggca tgtacaatgg agaccgttcg cgtaaagaaa tgctggttaa ttatggtttc
     2161 cgtttgccgt ctgctttgga caatcgtcct ctccgtcggg aggagtttga gagtcacgtt
     2221 catcagattg tttacgtttc agcgacacct ggtgactatg aaaatgaaca gaccgagaca
     2281 gtgattgagc aaatcattcg tccaacggga ctcttggatc cagaggtgga agtccgtccg
     2341 actatgggac agattgatga cctcttgggt gaaatcaatg cccgcgttga aaaaaatgag
     2401 cgtaccttta tcacaacttt gaccaagaaa atggcagagg atttgaccga ctacttcaag
     2461 gaaatgggta tcaaggtcaa gtacatgcac tcggatatca agaccttgga acggacggag
     2521 attatccgtg acctgcgctt gggtgtcttt gatgtcttgg tcggaattaa cctgctccgt
     2581 gaaggaattg acgttcctga agtgagcctc gtagctattc tcgatgctga caaggaaggt
     2641 ttccttcgca acgaacgtgg actcatccag actattggac gtgctgctcg taacagtgaa
     2701 ggccatgtca tcatgtatgc agacacggtt acccagtcta tgcaacgtgc tatcgatgaa
     2761 actgcccgcc gtcggaaaat tcagatggcc tataatgaag agcatggtat cgtaccacag
     2821 acaatcaaga aagaaatccg tgacctgatt gccgtgacca aggcagttgc taaggaagag
     2881 gacaaggaag tcgacatcaa tagcctcaac aaacaagagc gcaaagaact cgtcaagaaa
     2941 ctagaaaaac aaatgcaaga agccgtcgaa gtgcttgact ttgaactggc ggctcagatc
     3001 cgtgatatga tgttggaagt caaggccttg gattagggga atagtatgat ttatttaaga
     3061 aagttaaaga gagaagattt gatgtcttta tgggaaatgg cttattcaca acttaatcca
     3121 gtttggaaac agtatgatgc tccctattat gatgattatc agtatttttc aaattttaaa
     3181 gaattcgaac tacaaaaatc agaatccatt ttaagcaact caaatcgcct tggtattttt
     3241 gttgatgata aactagttgg gactgtttcg cgttattggg tatgtaaaca aacaagatgg
     3301 atggaattgg gaattggtat ttatgataaa aaattctgga acactggtat tgggaaagtt
     3361 gctatgttgc agtggataga taggacgttt caggattact tggagttgga gcatctgggt
     3421 ttgacaactt ggtcagggaa tattggtatg atgaaacttg ctgaaaaatt aagaatgaaa
     3481 aaagaagctc atattccaaa agttcgttat tatcaaggta aatattttga tagtattaaa
     3541 tatggtattt tgagagaaga ctgggagaaa ataaatgacg gttattatca aatcaatgga
     3601 aactcctaaa gagatagaag gtaaatccct tgttcactgg caaacgtgga gagagtctta
     3661 tgatgacctt ttgcctgcgg aatttcagga tacaatgaca ttagaaagat gtcgattctt
     3721 tagtcaaaag tatccagaaa atacattgat tgcaatggat ggcgtgaagg tggttggttt
     3781 tatcagttat ggcaattttc gtgatgagac tattcaagct ggtgaaatta ttgctttata
     3841 tgttttaaaa gactattatg gaaaaggtat cgcacaaaag ttaatgaaag cagctttgac
     3901 tgctcttaat catttttctg aaattttctt atgggtattg aaagataaca agcgcgccat
     3961 tgctttctat caaaaaatgg gttttacttt tgatggacaa gaaaaaatac ttgaacttgg
     4021 aaagcctata aaggaaaaac gaatggtgtt ctattctaaa taattttaaa aaagta
//
LOCUS       DAVLVP010000122         4031 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.122, whole genome shotgun sequence.
ACCESSION   DAVLVP010000122 DAVLVP010000000
VERSION     DAVLVP010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4031
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.122"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            81..878
                     /locus_tag="VYN69_001892"
     CDS             81..878
                     /locus_tag="VYN69_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000254803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEV1534105.1"
                     /translation="MVSSEFISKIEFACNKKESLYSQSKFKYAIRSMFAGAFLTFSTA
                     AGAVGADLINKIAPGSGRFLFPFVFAWGLAYIVFLNAELVTSNMMFLTAGSFLKKISW
                     RKTAEILLYCTLFNLIGALIAGWGFAHSAAYANLTHDSFISGVVEMKLGRSNELVLLE
                     AILANIFVNIAILSFILVKDGGAKLWLVLSAIYMFVFLTNEHIAANFASFAIVKFSVA
                     ADSIANFGVGNMLRHWGVTFIGNFIGGGLLMGLPYAFLNKNEDTYVD"
     gene            1026..1148
                     /locus_tag="VYN69_001893"
     CDS             1026..1148
                     /locus_tag="VYN69_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001855084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine sulfhydrylase"
                     /protein_id="HEV1534106.1"
                     /translation="MTCDFKSETLQLHAGQVVAPATKSRAVPIYQTTFFVFDDT"
     gene            1170..2210
                     /locus_tag="VYN69_001894"
     CDS             1170..2210
                     /locus_tag="VYN69_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001231001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase"
                     /protein_id="HEV1534107.1"
                     /translation="MRKPGNIYTRITNPTTAALEGGVEALATASGMTAVTYTILALAH
                     AGDHVVAASTIYGGTFNLLKEPLPRYGITTTFVDVDNLEEVEAAIKDNTKLVLIETLG
                     NSLINIPDLEKLAEIAHKHQIPLVSDNTFATPYLINVFSHGVDIAIHSVTKFIGGHGT
                     TIGGIIVDSGRFDWTASGKFPQFVDEGPSCHNLSYTRDVGAAAFIIAVRVQLLRDTGA
                     ALSPFNAFLLLQRLETSSLRVERHVQNAETIVDFLVNHPKVEKVNYPKLADSPYHALA
                     EKYLPKGVGSIFTFHVKGGEEEARKVIDNLEIFSDLANAADAKSLVVHPATTTHGQLS
                     EKDLEAAGVTPN"
     gene            2381..3655
                     /locus_tag="VYN69_001895"
     CDS             2381..3655
                     /locus_tag="VYN69_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001002592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2130 domain-containing protein"
                     /protein_id="HEV1534108.1"
                     /translation="MNEIKCPNCGEVFTVNESQYAELLSQVRTAEFDKELHDRMRQEL
                     ALAEQKAMNEQQTKLAQKDQEIAQLQSQIQNFDTEKELAKKEVEQTSHQALLAKDKEV
                     QALENQLATLRLEHENQLQKTLSDLEKERNQVKNQLLLQEKENELSLASVKQNYEAQL
                     KAASEQVEFYKNFKAQQSTKAIGESLEQYAESEFNKVRSFAFPNAYFEKDNKVSSRGS
                     KGDFIFRECDENGVEIISIMFEMKNEADGTEKKHKNADFYKELDKDRREKNCEYAVLV
                     TMLEADNDYFNTGIVDVSHEYEKMYVVRPQFFIQLIGLLRNAALNSLKYKQELALVRE
                     QNIDITHFEEDLDAFKLAFAKNYNSASTNFGKAIDEIDKAIKRMEEVKKFLTTSENQL
                     RLANNKLEDVSVKKLTRKNPTMKAKFEALKGE"
     gene            3667..>4031
                     /locus_tag="VYN69_001896"
     CDS             3667..>4031
                     /locus_tag="VYN69_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012512660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534109.1"
                     /translation="MEDDLNYENLMDDVTEAIKKFNLVIFIGAGVSIAQGYPNWNNYI
                     EHLIKYWQGQVLSVSGEKRLGREHHVVFDLISKSSISNKRKVDLVNYELKKVFGEDFE
                     KRRLDFEKGYFKNLLPYSIV"
BASE COUNT         1250 a          711 c          880 g         1190 t
ORIGIN      
        1 ttttatgaaa tacagaaaac gcttcctaaa acctaaagtt tgtgatataa ttatcaagaa
       61 aaaagattta ggagtttatt atggtttctt cagaatttat ctcaaagatt gaatttgctt
      121 gcaataagaa agaaagtctt tatagtcaaa gcaaatttaa gtatgcgatt cgttcgatgt
      181 tcgcaggtgc atttttaacc ttcagtactg ctgcaggtgc agttggggct gacttgatta
      241 ataaaattgc accaggtagt ggacgcttcc tctttccatt cgtttttgct tggggcttgg
      301 cctacattgt ttttttgaat gccgagttgg tcacttcaaa catgatgttc ttgactgctg
      361 gtagtttctt aaaaaaaatc tcttggagaa aaacagctga gattttacta tactgtacct
      421 tgttcaacct tatcggagcc ttgatagcag ggtggggctt tgctcattcg gcagcctatg
      481 cgaatctgac acacgatagt ttcatctcag gtgttgttga gatgaagtta ggccgctcaa
      541 atgaattggt cttgcttgag gcgattttgg caaatatttt cgtaaatatt gcgattctgt
      601 catttatttt ggtcaaagat ggtggtgcca aactttggct tgtgttgtca gctatttaca
      661 tgtttgtatt cttaacaaac gagcacattg cggcgaactt tgcttctttc gcgattgtga
      721 aattcagtgt tgctgcggat tcaattgcca acttcggtgt tggaaatatg cttcgccact
      781 ggggtgtgac tttcatcgga aactttatcg gaggaggcct cttgatgggt cttccatatg
      841 ccttcctcaa taaaaacgaa gatacttatg tagattaaga aaatgagcac gattgagtcg
      901 tgcttttttc attttcaaaa taagataata gctatttctt atatcaaaat atagaaaact
      961 gatatttgta aactataact caaggtgcta caatatcctt aataaaatga tatggaggtc
     1021 accttatgac ttgtgatttt aaatctgaaa ctctacaact acatgctggt caagttgtgg
     1081 ctccagctac taagtctcgt gcagtgccga tttatcaaac aacatttttt gtttttgatg
     1141 acacgtagga aggtgccgat ctgtttgcct tgaggaaacc agggaacatt tatactcgta
     1201 tcaccaatcc tacaacagct gcccttgaag gtggtgttga agcgctagca acagcatcag
     1261 gtatgactgc agtgacttat acgattttgg cgcttgccca tgctggtgac catgtagtgg
     1321 ctgcttcgac tatttacggt ggaaccttca atcttttgaa agaacccctt cctcgttatg
     1381 gtatcacaac aacctttgtc gatgttgata atttggagga agtagaagca gctatcaaag
     1441 acaataccaa gcttgtcttg attgaaacct tgggtaactc cttgattaat attccagacc
     1501 tggaaaaact ggcagagatt gctcataaac atcaaatccc acttgtgtca gacaatactt
     1561 ttgcaacacc ttatttgatt aacgtcttct ctcatggtgt tgacattgcc attcactctg
     1621 tgactaagtt tatcggtggg catggtacaa ctattggagg aataattgtc gatagtggtc
     1681 gttttgactg gacggcttca gggaaattcc ctcaatttgt tgacgagggt ccaagctgcc
     1741 acaatttgag ctatactcgt gatgtgggtg cagcagcctt tattatagct gttcgagttc
     1801 aattgcttcg tgatacaggt gcagccttgt caccattcaa tgctttcctc ttgctacaaa
     1861 gacttgaaac ctcttcactt cgtgtggaac gccatgtaca aaatgctgag acaattgttg
     1921 attttcttgt caaccatcct aaggtagaaa aggtaaatta tccaaaactt gcagatagtc
     1981 cttatcatgc cttggctgag aaatacttgc caaaaggtgt cggttcaatc tttaccttcc
     2041 acgtcaaagg tggcgaggaa gaagcacgca aggtcattga taatttagaa atcttttctg
     2101 accttgcaaa cgcggcagat gctaaatcgc ttgttgtcca tccagcaaca accactcacg
     2161 gtcaattgtc agaaaaagac ctagaagcag caggtgtcac accaaactaa attcgtttgt
     2221 caatcggtct tgaaaatgta gaagatttga ttgaagactt gcgcttggcc ttggaaaaaa
     2281 tttaaagtaa aagaagataa acagtgggct tcgactcact gtttttgatt ttccctcagg
     2341 catgatataa tggttaaaga agtctagaaa gaggaacgat atgaacgaaa tcaaatgtcc
     2401 caactgtggg gaagtcttta cagtaaatga aagccagtat gctgaacttt tgtcccaagt
     2461 gagaacggca gagtttgata aggaactaca cgataggatg aggcaggaac tggccttggc
     2521 tgagcaaaag gctatgaatg agcaacagac taaactggct cagaaggatc aagaaattgc
     2581 gcaattgcag agtcaaatcc aaaactttga tacagaaaaa gaattggcca agaaagaagt
     2641 tgaacagaca agccatcagg ccttattggc taaggacaag gaagtacagg ccttggaaaa
     2701 ccagttggcg accttgcgtt tggagcatga aaatcaattg caaaagaccc tttctgacct
     2761 agaaaaagaa cgcaatcagg tcaaaaatca gctcctactg caagaaaagg aaaatgagtt
     2821 gtctttggct tctgttaagc aaaactacga agcccagctc aaagcagcca gtgaacaagt
     2881 cgagttttat aagaatttta aggctcaaca atctacaaaa gcgattgggg aaagcctaga
     2941 acagtatgca gagagtgagt ttaacaaggt tcgtagtttc gcctttccaa atgcttactt
     3001 tgagaaggat aacaaggtct cttcgcgtgg gtctaaaggg gactttatct tccgtgagtg
     3061 tgatgaaaat ggagttgaaa tcatttctat catgtttgag atgaaaaacg aagcggacgg
     3121 aacagagaag aagcacaaga atgcagattt ttacaaggaa ttggacaagg accgtcggga
     3181 gaagaactgt gagtatgccg ttttggtgac catgcttgag gctgataatg actactttaa
     3241 cacagggatt gttgacgtca gtcacgagta tgaaaaaatg tatgttgttc gtcctcaatt
     3301 ctttatccaa ttgattggtc tcttacgtaa tgcggcgcta aattccctaa aatacaagca
     3361 ggagttggcc ttggttcgcg agcaaaatat tgacattacg cattttgagg aagacttgga
     3421 tgcctttaag ctagcttttg ctaagaacta taattcagct tcgactaact ttggaaaagc
     3481 tattgatgaa atcgacaagg ccatcaaacg catggaagag gttaagaaat tcctgactac
     3541 atctgaaaac caactccgtc tagctaacaa caaattggaa gatgtctctg ttaaaaaatt
     3601 gacccggaaa aatccaacaa tgaaagcgaa gttcgaagca ttgaaggggg agtgaggaaa
     3661 taagttatgg aagatgatct taattatgaa aatttgatgg atgatgtaac cgaggctata
     3721 aaaaagttta acttagtaat ttttattggc gcaggagttt caattgctca aggttatcct
     3781 aattggaaca attatattga acatcttatt aagtattggc aaggacaagt tttatcggta
     3841 agtggtgaaa aaaggcttgg aagagaacat cacgtagttt ttgatttaat tagcaaatct
     3901 agtattagta ataaacgtaa agtggatctt gtaaactatg aattaaaaaa agtttttggt
     3961 gaagattttg aaaagcgacg actagatttt gaaaaaggat attttaaaaa tctattacca
     4021 tattcaatag t
//
LOCUS       DAVLVP010000123         4021 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.123, whole genome shotgun sequence.
ACCESSION   DAVLVP010000123 DAVLVP010000000
VERSION     DAVLVP010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.123"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            236..1681
                     /gene="cps4A"
                     /locus_tag="VYN69_001897"
     CDS             236..1681
                     /gene="cps4A"
                     /locus_tag="VYN69_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000091082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     Cps4A"
                     /protein_id="HEV1534110.1"
                     /translation="MSRRFKKSRSQKVKRSVNIVLLTIYLLLVCFLLFLIFKYNILAF
                     RYLNLVVTALVLLVALVGLLLIIYKKAEKFTIFLLVFSILVSSVSLFAVQQFVGLTNR
                     LNATSNYSEYSLSVAVLADSEIENVTQLTSVTAPTGTDNENIQKLLADIKSSQNTDLT
                     VDQSSSYLAAYKSLIAGETKAIVLNSVFENIIESEYPDYASKIKKIYTKGFIKKVEAP
                     KTSKNQSFNIYVSGIDTYGPISSVSRSDVNILMTVNRDTKRILLTTTPRDSYVPIADG
                     GNNQKDKLTHAGIYGVDSSIHTLENLYGVDINYYVRLNFTSFLKLIDLLGGVDVHNDQ
                     EFSALHGKFHFPVGNVHLDSEQALGFVRERYSLADGDRDRGRNQQKVIVAILQKLTST
                     EALKNYSTIIDSLQDSIQTNMPLETMINLVNAQLESGGNYKVNSQDLKGTGRTDLPSY
                     AMPDSNLYVMEIDDSSLAVVKAAIQDVMEGR"
     gene            1683..2414
                     /locus_tag="VYN69_001898"
     CDS             1683..2414
                     /locus_tag="VYN69_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEV1534111.1"
                     /translation="MIDIHSHIVFDVDDGPKSREESKALLTESYRQGVRTIVSTSHRR
                     KGMFETPEEKIAENFLQVREIAKEVADDLVIAYGAEIYYTLDALEKLEKKEIPTLNDS
                     RYALIEFSMNTPYRDIHSALSKILMLGITPVIAHIERYDALENNEKRVRELIDMGCYT
                     QVNSSHVLKPKLFGERYKFMKKRAQYFLERDLVHVVASDMHNLDSRPPYMQQAYDIIA
                     KKYGAKKAKEFFVDNPRKIIMDQLI"
     gene            2423..3115
                     /gene="cpsC"
                     /locus_tag="VYN69_001899"
     CDS             2423..3115
                     /gene="cpsC"
                     /locus_tag="VYN69_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000664160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CpsC"
                     /protein_id="HEV1534112.1"
                     /translation="MKEHNTLEIDILQLFRALWKRKLVILLVAIITSSVAFAYSTFVI
                     KPEFTSTTRIYVVNRNQEEKSGLTNQDLQAGTYLVKDYREIILSQDVLEEVISDLKLD
                     LTPKGLANKIKVTVPVDTRIVSVSVNDRVPEEASRIANSLREVAAQKIISITRVSDVT
                     TLEEARPAISPSSPNIKRNILIGFLAGGVGTGVIVLLLELLDTHVKRPEDIEDTLQMT
                     LLGVVPNLSKLK"
     gene            3125..3808
                     /gene="cpsD"
                     /locus_tag="VYN69_001900"
     CDS             3125..3808
                     /gene="cpsD"
                     /locus_tag="VYN69_001900"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase
                     CpsD"
                     /protein_id="HEV1534113.1"
                     /translation="MPTLEIAQKKLEFIKKAEEYYNALCTNIQLSGDKLKVISVTSVN
                     PGEGKTTTSVNIARSFARAGYKTLLIDGDTRNSVMSGFFKSREKITGLTEFLSGTADL
                     SHGLCDTNIENLFVVQSGSVSPNPTALLQSKNFNDMIETLRKYFDYIIVDTAPIGIVI
                     DAAIITQKCDASILVTATGEVNKRDVQKAKQQLEQTGKLFLGVILNKFDVQHKKYGSY
                     GDYGNYGKK"
BASE COUNT         1280 a          643 c          828 g         1270 t
ORIGIN      
        1 tcttgttcat aattagattg aaactagaat agtacacctc tgcttctaaa acattgttag
       61 aaatcgattt gactgtcctg atcgttttgt catgttctta tttcatttta ctatattttt
      121 ggttcgtggg aagtctacta agatacttaa agatgcagat agtgaaaata aaggtgtaga
      181 cattaccgta aaaaagtgat ataatcgtat gatgttcaag gtataggtgt taatcatgag
      241 tagacgtttt aaaaaatcac gttcacagaa agtgaagcga agtgttaata tagttttgtt
      301 gactatttat ttattgttag tttgtttttt attgttctta atctttaagt acaatatcct
      361 tgcttttaga tatcttaatc tagtggtaac tgcgttagtc ctactagttg ccttggtagg
      421 gctactcttg attatctata aaaaagctga aaaatttact atttttctgt tggtgttttc
      481 tatccttgtc agctcagtgt cgctctttgc agtacagcag tttgttggac tgaccaatcg
      541 tttaaatgcg acttctaatt actcagaata ttcgctcagt gtcgctgttt tagcagatag
      601 tgagatcgaa aatgttacgc aactgacgag tgtgacagca ccgactggga ctgataatga
      661 aaatattcaa aaactactag ctgatatcaa gtcaagtcag aataccgatt tgacggtcga
      721 ccagagttcg tcttacttgg ctgcttacaa gagtttgatt gcaggggaga ctaaggccat
      781 tgtcctaaat agtgtctttg aaaatatcat cgagtcagag tatccagact acgcatcgaa
      841 gataaaaaag atttatacca agggattcat taaaaaagta gaagctccta agacgtctaa
      901 gaatcagtct ttcaatatct atgttagtgg aattgacacc tatggtccta ttagttcggt
      961 gtcgcgatca gatgtcaata tcctgatgac tgtcaatcga gataccaaga gaatccttct
     1021 tacaacaact cctcgagatt catatgttcc tattgctgat ggtggaaata atcaaaagga
     1081 taaattgact catgctggta tatatggggt tgattcatcc attcataccc tagagaacct
     1141 ttatggtgta gatattaatt attatgttcg tttgaatttt acctcatttt tgaaattgat
     1201 tgacttattg ggaggggtag atgttcataa tgatcaagag ttttcagctc tacatgggaa
     1261 gttccatttc ccagtaggga atgtccattt agactctgag caagctctag gttttgtacg
     1321 tgaacgctac tcactagccg atggagaccg tgaccgtggt cgcaaccaac aaaaggtgat
     1381 tgtggctatc cttcaaaaat taacgtcaac cgaagcgctg aaaaattata gtacgatcat
     1441 tgatagcttg caagattcta tccaaacaaa tatgccactt gagactatga taaatttggt
     1501 caatgctcag ttagaaagtg gagggaatta taaagtaaat tctcaagatt taaaaggtac
     1561 aggtcggacg gatcttcctt cttatgcaat gccagacagt aacctctatg tgatggaaat
     1621 agatgatagt agtttagctg tagttaaagc agctatacag gatgtgatgg agggtagatg
     1681 aaatgataga catccattcg catatcgttt ttgatgtaga tgacggtccc aagtcaagag
     1741 aggaaagcaa ggctctcttg acagaatcct acaggcaggg ggtgcgaacc attgtctcta
     1801 cctctcaccg tcgcaagggc atgtttgaaa ctccggaaga gaagatagca gaaaactttc
     1861 ttcaggttcg ggaaattgca aaagaagtag cagatgattt agtcattgct tatggcgcag
     1921 agatatacta tactctggat gctctagaaa agctagaaaa aaaagaaatt cctaccctca
     1981 atgatagtcg ttatgccttg atagagttta gtatgaacac tccttatcgc gatattcata
     2041 gcgccttgag caagattttg atgttgggaa ttactccagt cattgcccac attgagcgct
     2101 atgatgctct tgaaaataac gaaaaacgtg ttcgtgaact gattgatatg ggctgttaca
     2161 cgcaagtaaa tagttcacat gtcctcaaac ccaaactttt tggcgaacgt tataaattca
     2221 tgaaaaaaag agctcagtat tttttggaac gtgatttagt tcatgtagtt gcaagtgaca
     2281 tgcacaattt agacagtaga cctccatata tgcaacaggc atatgatatc attgctaaga
     2341 aatatggagc gaaaaaagcg aaagaatttt ttgtagataa tcccagaaaa attataatgg
     2401 atcaattaat ttaggagaaa atatgaagga acacaacact ttggaaatcg atatattgca
     2461 actattcaga gctttatgga aaagaaagtt ggtcatttta ttagtggcaa ttataacttc
     2521 ttcagttgct tttgcctaca gtacttttgt tatcaaacct gagtttacta gtacgactcg
     2581 gatttatgta gttaaccgta atcaggaaga gaagtctggt ttaaccaatc aagacttgca
     2641 ggcaggaact tatctggtaa aagactaccg tgagattatc ctttcgcagg atgttttgga
     2701 ggaagttatt tctgatttga aactagattt gacgccaaaa ggtttggcta ataaaattaa
     2761 agtaacagta ccagttgata cccgtattgt ctctgtttca gttaatgatc gagttcctga
     2821 agaggcaagc cgtatcgcta actctttgag agaagtagct gctcaaaaaa ttatcagtat
     2881 tactcgtgtt tctgatgtga caacactgga ggaggcaaga ccggcgatat caccgtcttc
     2941 gccaaatatt aaacgcaata tactaattgg ttttttggca gggggggttg gaactggtgt
     3001 tatagttctt cttcttgaac ttttggacac tcatgtgaaa cgtccggaag atatcgaaga
     3061 tacactgcag atgacacttt tgggagttgt accaaacttg agtaagttga aataggagag
     3121 aggaatgccg acattagaaa tagcacaaaa aaaactggag ttcattaaga aggcagaaga
     3181 atattacaat gccttgtgta caaatataca gttgagcgga gataaactaa aagtaatttc
     3241 cgttacttct gttaaccctg gggaaggaaa aacaactact tccgtaaata tagcaaggtc
     3301 gtttgcgcgt gcaggctata aaactctttt gatcgatggc gatactcgaa attcagttat
     3361 gtcaggattt tttaaatctc gtgaaaaaat tacagggcta acagaatttt tatctgggac
     3421 agctgattta tctcacggtt tatgtgatac aaatattgaa aatttatttg tagttcaatc
     3481 ggggtctgta tcaccaaacc ctacagcctt gttacaaagt aaaaatttta atgatatgat
     3541 tgaaacattg cgtaaatatt ttgattatat cattgttgat acagcaccta ttggaattgt
     3601 tattgatgcg gcaattatca ctcaaaagtg tgatgcgtcc atcttggtaa cagcaacagg
     3661 tgaggtgaat aaacgtgatg tccaaaaagc gaaacaacaa ttagaacaaa cagggaaact
     3721 gtttttaggt gtcattctca ataaatttga tgttcaacat aaaaaatatg gttcttacgg
     3781 tgactatggt aattatggta aaaaataact taggaaagat tttatggatg aaaaaggatt
     3841 gaaaattttt ctggcagtat tacagagtat tattgtcatt ttattggttt attttcttag
     3901 ctttgttaga gagacagaac ttgaacgttc ttcgatggtt atactatacc ttctccactt
     3961 ttttgtattc tattttagtt cctatggtaa taattttttt aaaagagggc acttagttga
     4021 g
//
LOCUS       DAVLVP010000124         4013 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.124, whole genome shotgun sequence.
ACCESSION   DAVLVP010000124 DAVLVP010000000
VERSION     DAVLVP010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 4013)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4013)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4013
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.124"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            53..1462
                     /locus_tag="VYN69_001901"
     CDS             53..1462
                     /locus_tag="VYN69_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000728302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1534114.1"
                     /translation="MKKKLVFPNLFWWGAASSGPQTEGQYGKVHENVMDYWFKTHPED
                     FFDNVGPLVASNFFHTYTEDFHLMKEIGVNSFRTSIQWSRLIKNLETGEPDPKGIAFY
                     NAIIEEAKKNQMDLVMNLHHFDLPVELLQKYGGWESKHVVELFVKFAKTAFTCFGDKV
                     HYWTTFNEPMVIPEAGYLYAFHYPNLKGKGKEAVQVIYNLNLASAKVIQLYRSLELDG
                     KIGIILNLTPAYPRSNSPEDLEASRFTDDFFNKVFLNPAVKGTFPERLVKQLERDGVL
                     WSHTEKELQLMKSNTVDFLGVNYYHPKRVQAQANPEEYQTPWMPDQYFKEYEWLERRM
                     NPYRGWEIFPKAIYDIAMIVKEEYGNIPWFISENGMGVENEARFIDENGVIDDVYRIE
                     FYEEHLRWLHKAIEEGSHCFGYHAWTAFDCWSWNNAYKNRYGFISVDLETQKRTIKSS
                     GRWYRKVSDNNGFEVEIEE"
     gene            1480..2775
                     /locus_tag="VYN69_001902"
     CDS             1480..2775
                     /locus_tag="VYN69_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEV1534115.1"
                     /translation="MKNSKFIDQFATFAGKLGNQIHLKTLRDAFVTVMPLYILAGLIV
                     LLNNTVFKWIFQGDTLTRFQYWGITIANGTLSISGMIIAVMVGYFLAKNRDFENPLAA
                     SMLSLVSLIVMMPNTVSVVPDGAKDAVNISGVLSFNNTGTGAMFAGVIVAIIATELFI
                     ELSNVKALQMNLGENIPPAVSRSFSVLLPVMTVISLFGVVSALLFNITGMNLISIITI
                     FIQEPIRHIGTSLIGVIIIYSLGNMLWLFGIHQAVIYSAILEPLLLINITENITAANN
                     GQAIPHIINLSQIQTFALMGGSGSTLCLLIATFLVSRNAVSKNVAKLSFGPGIFNINE
                     PVLFGYPIVYNISLAIPFITVPVLGILISYLATVTGFMSPAFIQVPWTTPVFLNAWLA
                     TAGDVRAVLVQFIIFALGVLLYIPFIKVNDKVVEQEMES"
     gene            2780..3088
                     /locus_tag="VYN69_001903"
     CDS             2780..3088
                     /locus_tag="VYN69_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEV1534116.1"
                     /translation="MKQILLVCNAGMSTSMLVKKMQQSATERGIEISIQAKSMTEAKK
                     NIHEADVILIGPQIRYELLAVKEIAGNIPVDTIDMRDYGMMNGAKVLEQALAWIGEIR
                     "
     gene            3085..3393
                     /locus_tag="VYN69_001904"
     CDS             3085..3393
                     /locus_tag="VYN69_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEV1534117.1"
                     /translation="MTEEMEYICFQLIANSGAAKSSFIEAIQLAKAGNLKEAKIKIEE
                     AEDSLVEAHKIHSNLIQKEATGEKIGFSLLFMHAEDQMASTEIIQLLSKEFIDLYQNK
                     "
BASE COUNT         1345 a          672 c          763 g         1233 t
ORIGIN      
        1 ttttaaaatt atgctatact ttatgtatag aataatagaa aggaggttga gcatgaaaaa
       61 aaagttagta tttcctaatc tgttttggtg gggagctgct tctagcggac ctcagacaga
      121 gggtcaatat ggaaaagtac atgaaaatgt gatggactac tggttcaaaa cgcatccaga
      181 agattttttc gataatgtcg gacctcttgt agccagtaac ttttttcata cttacaccga
      241 agatttccac ttgatgaagg aaattggagt taattctttc cgcacttcca tccaatggag
      301 tcgactcatc aagaatttag agacaggtga gcctgatcca aaaggtattg ctttctacaa
      361 tgccatcatt gaagaagcta aaaagaacca gatggatctt gtgatgaatt tacatcattt
      421 tgatttacca gtggaacttc ttcaaaaata cggtggttgg gaaagcaaac atgtagtgga
      481 gttattcgtg aagtttgcca agactgcttt cacatgcttt ggagataagg ttcattactg
      541 gacaactttc aatgagccaa tggtcattcc agaagcagga tacttatatg ctttccatta
      601 tcccaatcta aaaggaaagg gaaaagaggc cgtacaagtc atctataatc taaaccttgc
      661 tagtgcaaaa gtgattcaac tatatcgctc attagaactt gatggaaaga ttgggattat
      721 tttaaacttg acacctgctt atccaagaag taattctcca gaagacttag aagcaagtcg
      781 atttacagat gacttcttta acaaagtctt cttgaatcca gctgttaaag gaactttccc
      841 agaaagattg gtaaaacagc tagagagaga tggcgtgtta tggagtcata ccgaaaaaga
      901 gcttcaactg atgaaatcaa atacggttga ttttcttgga gtaaactact accatccaaa
      961 acgtgttcaa gcacaagcaa atcctgagga atatcagacg ccctggatgc cagaccaata
     1021 cttcaaagag tatgaatggc tggagcgtcg catgaatcca tatcgtggtt gggaaatttt
     1081 tccgaaagcc atttatgata ttgctatgat tgtgaaggaa gaatatggta atatcccatg
     1141 gtttatcagt gaaaacggaa tgggtgttga aaacgaagca cggtttatcg atgaaaatgg
     1201 agttatcgat gacgtgtatc gtattgaatt ttatgaagaa catttaagat ggctacataa
     1261 agccattgaa gagggaagtc actgttttgg ataccacgct tggaccgcat ttgattgctg
     1321 gtcttggaat aatgcatata agaatcgtta cggatttatc tccgttgatt tagaaacgca
     1381 aaagagaacc atcaagagct caggaagatg gtatcgcaaa gtaagtgaca ataacggttt
     1441 tgaagtagaa attgaggagt aaagagagga gatattcaga tgaaaaactc aaaatttata
     1501 gaccaatttg ccacctttgc tggtaaacta gggaaccaaa ttcatttaaa aaccctaaga
     1561 gatgcattcg taacagtaat gccattatat attttggcag gcttaatcgt tcttttgaac
     1621 aacacggtat ttaagtggat tttccaaggg gatacattaa caagattcca atattgggga
     1681 ataacaattg caaacggtac tttaagtatt tcaggtatga ttattgctgt aatggttggt
     1741 tatttcttag ctaaaaacag agatttcgaa aacccgttag cagcatcaat gctatcatta
     1801 gtttctttaa ttgtgatgat gccaaataca gtttctgtag ttcctgacgg agcaaaagat
     1861 gcggtaaaca tttcaggtgt tctttcattc aacaacacag gtacaggcgc aatgttcgcc
     1921 ggggttatcg tagcgattat tgcaacagaa ttattcattg aattatcaaa cgttaaagct
     1981 ttacaaatga accttggtga aaatattcca ccagctgtta gtagatcatt tagcgtatta
     2041 cttccagtca tgaccgtcat ctccttattt ggggttgttt cagcattatt attcaatata
     2101 actggaatga acttaatctc aatcattaca atctttattc aagaaccaat tcgtcatatt
     2161 ggtacaagct taatcggggt cattattatt tactctttag gaaatatgtt atggctattt
     2221 ggtattcacc aagcagttat ttacagtgcc atcctagaac cattactatt aattaacatt
     2281 actgaaaaca tcactgcagc aaataatgga caagccattc cacacatcat caacctatca
     2341 caaatacaaa cattcgcttt aatgggtggt agtggatcta cattatgttt attaatagca
     2401 acattcttag tgagtcgcaa tgctgtctct aaaaacgtgg ctaaattatc ttttggacct
     2461 ggtatcttca atatcaatga accagtatta ttcggttacc caatcgttta taacatttca
     2521 ttagctattc catttatcac agttccagtc cttggtattt taatcagcta cttagcaaca
     2581 gttacaggat tcatgagtcc tgcatttata caagttcctt ggactacacc agtattctta
     2641 aatgcatggt tagcaacagc aggggacgtg agagcagttc tagttcaatt catcatcttt
     2701 gcacttggag ttcttctata cattccattt atcaaagtta atgacaaagt tgttgaacaa
     2761 gaaatggaaa gttaagatta tgaaacaaat tttattagta tgtaatgcgg gaatgtcgac
     2821 aagtatgctt gttaaaaaaa tgcaacaaag tgcgactgaa cgtgggattg aaatctctat
     2881 ccaagcaaaa tcaatgacag aagctaaaaa gaatatccat gaagcagatg tcattctaat
     2941 tgggccacaa attcgttatg agttacttgc tgtgaaagaa attgcaggaa atatccctgt
     3001 agataccatt gatatgcggg attacggaat gatgaatgga gcaaaagttc tggaacaagc
     3061 acttgcatgg ataggagaaa taagatgaca gaagaaatgg aatatatctg ttttcagctc
     3121 attgccaata gtggagcagc caaatcatcc tttattgaag ccatccaact agctaaggca
     3181 ggaaatttaa aagaagcgaa aattaaaatt gaagaagcgg aagactcctt agtagaagcg
     3241 cacaagattc actctaatct catacaaaaa gaagctactg gagaaaaaat aggattctcc
     3301 ttgctattta tgcatgcaga agaccaaatg gcttcaacag agattattca gttgctatct
     3361 aaagaattca tcgatctcta tcaaaataaa taggaacact gtcattccca tctgtcttta
     3421 ttaatgacga ctacattgta ggaacctcca aaagttggac aaaatgattg aaaggattgg
     3481 atcctgcatt acacaagatc caatcctttt cttttactct agtttttata atatgttgaa
     3541 tctagaatag tccactgtag tttctaaaaa attgctggaa atggatgtta agctccaatt
     3601 tatttgttta tatcttattt cagtttacta tactagctta ttattctagt gaaaaattga
     3661 atgagccact atttatttaa tatgtatgat gagggaatga ctcaaaaact tcttgtcgat
     3721 ataagaatat ctgagatagt attttaaaac gaaaggacta taaaagctca tttccttcgc
     3781 acttatttta tagagatcta tcattatact ctttagaaat caaaaagcaa cacaccttgt
     3841 catcgtttta tcgtaaatct gttaatgagg gggctagtct tatctgcaat ctcaaatcag
     3901 tgttttgagc tgaatggcac tcgttttcta gtttgctctt tgattttaat tgggccttag
     3961 taaatactac tcttaaatta aactatagtg aattgaaata agatgtgaac aac
//
LOCUS       DAVLVP010000125         3857 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.125, whole genome shotgun sequence.
ACCESSION   DAVLVP010000125 DAVLVP010000000
VERSION     DAVLVP010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3857)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.125"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..418
                     /locus_tag="VYN69_001905"
     CDS             <1..418
                     /locus_tag="VYN69_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534118.1"
                     /translation="KNKADQDSKPDEDKEHDEVSEPTHPESDEKENHAGLNPSADNLY
                     KPSTDTEETEEEAEDTTDEAEIPQVENSVINAKIADAEALLEKVTDPSIRQNAMETLT
                     GLKSSLLLGTKDNNTISAEVDSLLALLKESQPAPIQ"
     gene            628..3747
                     /gene="phtE"
                     /locus_tag="VYN69_001906"
     CDS             628..3747
                     /gene="phtE"
                     /locus_tag="VYN69_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000672589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtE"
                     /protein_id="HEV1534119.1"
                     /translation="MKFSKKYIAAGSAVIVSLSLCAYALNQHRSQENKDNNRVSYVDG
                     SQSSQKSENLTPDQVSQKEGIQAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDALFSE
                     ELLMKDPNYQLKDADIVNEVKGGYIIKVDGKYYVYLKDAAHADNVRTKDEINRQKQEH
                     VKDNEKVNSNVAVARSQGRYTTNDGYVFNPADIIEDTGNAYIVPHGGHYHYIPKSDLS
                     ASELAAAKAHLAGKNMQPSQLSYSSTASDNNTQSVAKGSTSKPANKSENLQSLLKELY
                     DSPSAQRYSESDGLVFDPAKIISRTPNGVAIPHGDHYHFIPYSKLSALEEKIARMVPI
                     SGTGSTVSTNAKPNEVVSSLGSLSSNPSSLTTSKELSSASDGYIFNPKDIVEETATAY
                     IVRHGDHFHYIPKSNQIGQPTLPNNSLATPSPSLPINPGTSHEKHEEDGYGFDANRII
                     AEDESGFVMSHGDHNHYFFKKDLTEEQIKAAQKHLEEVKTSHNGLDSLSSHEQDYPSN
                     AKEMKDLDKKIEEKIAGIMKQYGVKRESIVVNKEKNAIIYPHGDHHHADPIDEHKPVG
                     IGHSHSNYELFKPEEGVAKKEGNKVYTGEELTNVVNLLKNSTFNNQNFTLANGQKRVS
                     FSFPPELEKKLGINMLVKLITPDGKVLEKVSGKVFGEGVGNIANFELDQPYLPGQTFK
                     YTIASKDYPEVSYDGTFTVPTSLAYKMASQTIFYPFHAGDTYLRVNPQFAVPKGTDAL
                     VRVFDEFHGNAYLENNYKVGEIKLPIPKLNQGTTRTAGNKIPVTFMANAYLDNQSTYI
                     VEVPILEKENQTDKPSILPQFKRNKAQENSKLDEKVEEPKTSEKVEKEKLSETGNSTS
                     NSTLEEVPTVDPVQEKVAKFAESYGMKLENVLFNMDGTIELYLPSGEVIKKNMADFTG
                     EAPQGNGENKPSENGKVSTGTVENQPTENKPADSLPEAPNEKPVKPENSTDNGMLNPE
                     GNVGSDPMLDPALEEAPAVDPVQEKLEKFTASYGLGLDSVIFNMDGTIELRLPSGEVI
                     KKNLSDLIA"
BASE COUNT         1444 a          626 c          752 g         1035 t
ORIGIN      
        1 taaaaataag gcagaccaag atagtaaacc tgatgaagat aaggaacatg atgaagtaag
       61 tgagccaact caccctgaat ctgatgaaaa agagaatcac gctggtttaa atccttcagc
      121 agataatctt tataaaccaa gcactgatac ggaagagaca gaggaagaag ctgaagatac
      181 cacagatgag gctgaaattc ctcaagtaga gaattctgtt attaacgcta agatagcaga
      241 tgcggaggcc ttgctagaaa aagtaacaga tcctagtatt agacaaaatg ctatggagac
      301 attgactggt ctaaaaagta gtcttcttct cggaacgaaa gataataata ctatttcagc
      361 agaagtagat agtctcttgg ctttgttaaa agaaagtcaa ccggctccta tacagtagta
      421 aaatgaatgg agcatatttt atggagaagt aacctttcgt gttacttctc ttttttagaa
      481 aaacgtaaca gaaacttgac aaaagtaatt ttaaatagta aactatttac tggttaatta
      541 aatggttaaa taaccggttt agaaaactat ttaataaagt aaaagaagtt gagaaaaaac
      601 ttcatcattt attgaaatga gggatttatg aaatttagta aaaaatatat agcagctgga
      661 tcagctgtta tcgtatcctt gagtctatgt gcctatgcac taaaccagca tcgttcgcag
      721 gaaaataagg acaataatcg tgtctcttat gtggatggca gccagtcaag tcagaaaagt
      781 gaaaacttga caccagacca ggttagccag aaagaaggaa ttcaggctga gcaaattgta
      841 atcaaaatta cagatcaggg ctatgtaacg tcacacggtg accactatca ttactataat
      901 gggaaagttc cttatgatgc cctctttagt gaagaactct tgatgaagga tccaaactat
      961 caacttaaag acgctgatat tgtcaatgaa gtcaagggtg gttatatcat caaggtcgat
     1021 ggaaaatatt atgtctacct gaaagatgca gctcatgctg ataatgttcg aactaaagat
     1081 gaaatcaatc gtcaaaaaca agaacatgtc aaagataatg agaaggttaa ctctaatgtt
     1141 gctgtagcaa ggtctcaggg acgatatacg acaaatgatg gttatgtctt taatccagct
     1201 gatattatcg aagatacggg taatgcttat atcgttcctc atggaggtca ctatcactac
     1261 attcccaaaa gcgatttatc tgctagtgaa ttagcagcag ctaaagcaca tctggctgga
     1321 aaaaatatgc aaccgagtca gttaagctat tcttcaacag ctagtgacaa taacacgcaa
     1381 tctgtagcaa aaggatcaac tagcaagcca gcaaataaat ctgaaaatct ccagagtctt
     1441 ttgaaggaac tctatgattc acctagcgcc caacgttaca gtgaatcaga tggcctggtc
     1501 tttgaccctg ctaagattat cagtcgtaca ccaaatggag ttgcgattcc gcatggcgac
     1561 cattaccact ttattcctta cagcaagctt tctgctttag aagaaaagat tgccagaatg
     1621 gtgcctatca gtggaactgg ttctacagtt tctacaaatg caaaacctaa tgaagtagtg
     1681 tctagtctag gcagtctttc aagcaatcct tcttctttaa cgacaagtaa ggagctctct
     1741 tcagcatctg atggttatat ttttaatcca aaagatatcg ttgaagaaac ggctacagct
     1801 tatattgtaa gacatggtga tcatttccat tacattccaa aatcaaatca aattgggcaa
     1861 ccgactcttc caaacaatag tctagcaaca ccttctccat ctcttccaat caatccagga
     1921 acttcacatg agaaacatga agaagatgga tacggatttg atgctaatcg tattatcgct
     1981 gaagatgaat caggttttgt catgagtcac ggagaccaca atcattattt cttcaagaag
     2041 gacttgacag aagagcaaat taaggctgcg caaaaacatt tagaggaagt taaaactagt
     2101 cataatggat tagattcttt gtcatctcat gaacaggatt atccaagtaa tgccaaagaa
     2161 atgaaagatt tagataaaaa aatcgaagaa aaaattgctg gcattatgaa acaatatggt
     2221 gtcaaacgtg aaagtattgt cgtgaataaa gaaaaaaatg cgattattta tccgcatgga
     2281 gatcaccatc atgcagatcc gattgatgaa cataaaccgg ttggaattgg tcattctcac
     2341 agtaactatg aactgtttaa acccgaagaa ggagttgcta aaaaagaagg gaataaagtt
     2401 tatactggag aagaattaac gaatgttgtt aatttgttaa aaaatagtac gtttaataat
     2461 caaaacttta ctctagccaa tggtcaaaaa cgcgtttctt ttagttttcc gcctgaattg
     2521 gagaaaaaat taggtatcaa tatgctagta aaattaataa caccagatgg aaaagtattg
     2581 gagaaagtat ctggtaaagt atttggagaa ggagtaggga atattgcaaa ctttgaatta
     2641 gatcaacctt atttaccagg acaaacattt aagtatacta tcgcttcaaa agattatcca
     2701 gaagtaagtt atgatggtac atttacagtt ccaacctctt tagcttacaa aatggccagt
     2761 caaacgattt tctatccttt ccatgcaggg gatacttatt taagagtgaa ccctcaattt
     2821 gcagtgccta aaggaactga tgctttagtc agagtgtttg atgaatttca tggaaatgct
     2881 tatttagaaa ataactataa agttggtgaa atcaaattac cgattccgaa attaaaccaa
     2941 ggaacaacca gaacggccgg aaataaaatt cctgtaacct tcatggcaaa tgcttatttg
     3001 gacaatcaat cgacttatat tgtggaagta cctatcttgg aaaaagaaaa tcaaactgat
     3061 aaaccaagta ttctaccaca atttaaaagg aataaagcac aagaaaactc aaaacttgat
     3121 gaaaaggtag aagaaccaaa gactagtgag aaggtagaaa aagaaaaact ttctgaaact
     3181 gggaatagta ctagtaattc aacgttagaa gaagttccta cagtggatcc tgtacaagaa
     3241 aaagtagcaa aatttgctga aagttatggg atgaagctag aaaatgtctt gtttaatatg
     3301 gacggaacaa ttgaattata tttaccatcg ggagaagtca ttaaaaagaa tatggcagat
     3361 tttacaggag aagcacctca aggaaatggt gaaaataaac catctgaaaa tggaaaagta
     3421 tctactggaa cagttgagaa ccaaccaaca gaaaataaac cagcagattc tttaccagag
     3481 gcaccaaacg aaaaacctgt aaaaccagaa aactcaacgg ataatggaat gttgaatcca
     3541 gaagggaatg tggggagtga ccctatgtta gatccagcat tagaggaagc tccagcagta
     3601 gatcctgtac aagaaaaatt agaaaaattt acagctagtt acggattagg cttagatagt
     3661 gttatattca atatggatgg aacgattgaa ttaagattgc caagtggaga agtgataaaa
     3721 aagaatttgt ctgatctcat agcgtaagga atagcagtag aaaaagtctg aatcaaaaat
     3781 gaagttctct caaaagttag aaataaaact ctgactttgg gagaatttca ttttattatt
     3841 aatatataaa atttctt
//
LOCUS       DAVLVP010000126         3793 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.126, whole genome shotgun sequence.
ACCESSION   DAVLVP010000126 DAVLVP010000000
VERSION     DAVLVP010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.126"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..513
                     /locus_tag="VYN69_001907"
     CDS             <1..513
                     /locus_tag="VYN69_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000636508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HEV1534120.1"
                     /translation="APTFRDDGDASYLDINFERLLQSIEQEIKKKCKILIRLHPNDSH
                     FSNNISFNHDIIDVTLFSDMQELILLADVLLTDYSSAIFDFMLLNKPYVRYVNDLEKY
                     AELRGVSDTYYELPDSIIKTAEELYDLLPKKIENFDYDSIKKYRNEILCPIFNGTASE
                     NVGRRIIQEL"
     gene            549..1577
                     /locus_tag="VYN69_001908"
     CDS             549..1577
                     /locus_tag="VYN69_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEV1534121.1"
                     /translation="MKNNDLKIGSGAIHQISATLSQNNISGKILYCADPVVDDLYGSI
                     VRSQIEEIGRVKEESCNYNTIAYAMNIAERAIATDIDCIVGMGGGRVLDVCKYASFIS
                     KRPYLSIPTTAANDGIASPVAVLKRQDDRPKSLGAAIPSMTLIDIDVIASGPIQNIKA
                     GIGDTISNYTALKDWELAVERGKDEMHGFAYLMSQNSLDALMKTKYNSITPDFIEVLV
                     NSLVLSGIAMDFAGSSRPVSGSEHLFSHALDYYGSTRNLHGIQVALGTVAVLKLIENS
                     VDTVVDYLQRFEVHINPKLLGIDEELFIYCMQHATKMRSNRYTYLHEVDLSTDRLKQI
                     YKELISEL"
     gene            1593..2297
                     /locus_tag="VYN69_001909"
     CDS             1593..2297
                     /locus_tag="VYN69_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000645462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocholine cytidylyltransferase family
                     protein"
                     /protein_id="HEV1534122.1"
                     /translation="MKALILAAGLGTRLAPITNEVPKSLVPVNGKPILMKQIENLYQN
                     NITDITIIAGYKSSVLTDAVTEKYPEINIIDNVDFKTTNNMYSAYLGKAAMGDSDFLM
                     MNADVFYDASVIKSLLLHKAPNAIVTDLGIYIEESMKVVEKNGRLVEISKQISPEETL
                     GVSIDVYKFSYEAGARFFEKCKEFIEDKRELQMWSEVALNAILSEVEFVACPLEGRWL
                     EIDNHEDLVAAEKLFA"
     gene            2312..3145
                     /locus_tag="VYN69_001910"
     CDS             2312..3145
                     /locus_tag="VYN69_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000779205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HEV1534123.1"
                     /translation="MKLTNRVDYFGADISELQNKKLFLFDMDGTIYEEDRLFEGTLEL
                     LDYIHNIGGEYIFITNNSSKSVVDYVEKVNRLGIKAERDNFFTSAQATIVYIKENYPK
                     SKVYCQGTKSLIKELSDAGIDVTEQVSADIDVVLVGFDTELTSDKIRNTCEILSTKDV
                     PFIATNPDIRCPVSFGFIPDCGSICDMISKSVDRKPVYIGKPEPTMVDIVRKKLNYSL
                     FETVVIGDRLYTDIMTGINAGVTSVCVLTGEATVNDIQQGSIKPTYTFKNVKEMWKGI
                     V"
     gene            complement(3234..3626)
                     /locus_tag="VYN69_001911"
                     /pseudo
     CDS             complement(3234..3626)
                     /locus_tag="VYN69_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105201.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1256 a          532 c          747 g         1258 t
ORIGIN      
        1 gcccctactt tcagagatga tggggatgca tcttatttag atataaattt tgaacggctt
       61 ttgcaaagta ttgaacaaga aattaagaag aaatgtaaaa ttttaattag attacatcct
      121 aatgactctc attttagtaa taatatttct tttaatcatg acataattga tgttacgcta
      181 ttctcagata tgcaggaact tatattattg gcagatgttt tactgacaga ttattcttcc
      241 gcgatatttg attttatgct tttaaacaag ccttatgtca gatatgtgaa tgatttagaa
      301 aaatatgctg agcttagggg ggtatcagat acatattatg aattgccgga ttctattatt
      361 aaaactgctg aagaattgta tgacttactg cctaaaaaaa tagaaaattt tgattatgat
      421 tctataaaaa aatatagaaa tgaaatactg tgtccaatct ttaatggaac cgctagtgaa
      481 aatgtaggaa ggagaataat tcaagagttg tgaagtaaaa cattgggtta tttttaaggt
      541 aacgatattt gaaaaataat gatttaaaga taggaagtgg agctattcat caaatctccg
      601 ctacactttc tcaaaataat atttcgggaa agattttata ttgtgctgat ccagttgtcg
      661 atgacttgta cggttcgata gtacgttcac aaatagagga aattggtcgg gtaaaggaag
      721 aaagttgcaa ttataatact attgcttatg cgatgaatat agcagaaagg gctattgcca
      781 cagatattga ctgtattgtg ggaatgggag gaggtcgtgt tttagatgtc tgcaagtatg
      841 catcttttat ttcgaaacgt ccttacctat cgattccgac aacagcagca aatgatggca
      901 ttgcttcccc agttgctgtt ttgaaaaggc aagatgatag accaaaaagc ttaggggcgg
      961 ctatcccctc aatgacacta attgatattg atgttattgc atcaggacct atacaaaata
     1021 taaaagctgg tatcggtgat acaatatcca attacactgc attgaaagat tgggagttgg
     1081 cagttgagcg agggaaagat gagatgcatg gttttgcata tctaatgtcg caaaattctt
     1141 tagatgcttt aatgaaaacg aagtataatt ctattacccc tgattttatt gaagttttgg
     1201 taaactcttt agttttatca ggcattgcaa tggattttgc gggaagtagt agacctgtca
     1261 gtgggtcaga gcacttattt agtcatgcat tagattacta tggttctaca aggaatcttc
     1321 atggaattca ggttgcatta ggtacagtcg cagttttgaa attaattgaa aattctgttg
     1381 atactgtagt ggattacttg caaagatttg aggttcatat taacccgaaa cttttgggga
     1441 tagatgaaga gttgtttatt tattgtatgc aacatgctac aaaaatgaga agtaatcgct
     1501 atacctatct gcatgaagtt gatcttagca cagatagatt gaaacaaata tataaggaac
     1561 tcataagcga gttgtagaaa agagaggaaa aaatgaaagc acttatttta gcagcagggt
     1621 taggaacacg tctagctcca attaccaatg aggtaccaaa atctttggta ccagtcaatg
     1681 gtaagccaat tttgatgaaa caaattgaaa atttatatca aaataatatt acagatatta
     1741 cgattattgc tggatataag tcatctgtat taacagatgc agttactgaa aagtatccag
     1801 aaattaatat tattgataat gttgatttta aaacgactaa taatatgtat tcagcctatc
     1861 taggaaaagc tgcaatgggt gatagtgact tcttaatgat gaatgcagat gtattttatg
     1921 atgcttctgt tattaaaagt ctgttgcttc ataaagctcc aaatgcaatt gtaactgatt
     1981 taggtattta tattgaagag tctatgaaag tcgtagaaaa aaatggtcgt ttagtagaaa
     2041 tttctaaaca gatttcacct gaggaaactt taggagtttc tattgatgtt tataaattct
     2101 cttatgaagc aggtgctcga ttctttgaaa agtgtaagga atttattgaa gataaacgag
     2161 aacttcaaat gtggagtgag gttgctctta atgcaattct ttctgaagtt gaatttgtag
     2221 catgtccatt agagggtcgt tggttagaaa ttgataatca tgaagactta gttgctgcag
     2281 aaaaactatt tgcttgaacc gaatacggag aatgaaattg acaaatagag ttgattattt
     2341 tggtgctgat attagtgaac ttcagaataa aaaattattc ttatttgata tggatggtac
     2401 catttatgaa gaagataggt tgtttgaggg tactctcgaa ttattagact atattcataa
     2461 tattggcggt gagtatattt ttattacaaa caattcatct aagtctgttg ttgactatgt
     2521 agaaaaagtt aacagattag gtattaaagc tgaacgagat aattttttta cctctgctca
     2581 agccacaatt gtttatatta aagaaaatta tcctaaatct aaagtttatt gccaaggaac
     2641 aaaatctttg ataaaagaac tatctgacgc aggaattgat gtaactgagc aagttagtgc
     2701 tgatatagat gttgttcttg ttggttttga tacagaatta accagtgata aaattcgcaa
     2761 tacctgcgag attctatcaa caaaggacgt acctttcata gctactaacc ctgatattcg
     2821 ctgcccagta tcgtttggat tcatcccaga ttgtggttct atttgtgata tgattagtaa
     2881 atcagtcgat aggaaacctg tttacatagg taaacctgaa cctacgatgg ttgatattgt
     2941 tcgaaaaaaa ttaaattatt ctctatttga aacagttgtg attggagatc gcttgtatac
     3001 ggatatcatg actggtataa atgcaggagt aacttcagtt tgtgtgctga caggagaagc
     3061 aacggtgaat gatattcaac aaggtagtat aaaaccgact tatacattta aaaacgtgaa
     3121 agaaatgtgg aaaggaattg tctgattttt tcattctgaa tagtgaattc atgaatactg
     3181 tcaaaatact cgtatgtatt taaattgatt ttattattag acttcctgca aaactagaat
     3241 cctagttcat gattgataat agcagcaatc aaattcattc gtaatccgaa gcgtttacga
     3301 tgatttcgat aggttgttga aatcatttta aacgttttta ctttggcaaa gatgttctca
     3361 accttgcttc tctccttgga tagcgcatgg ttacaggctt tatcttcaac tgttagcggt
     3421 ttgagtttgc tggatttacg tggagtttat gcttgaggat atatcttcat gagcccttga
     3481 taaccactgt cagccaagat tttaccagct tgtccgatat ttctgcgact cattttgaac
     3541 aactttatat catgacaata gttcacagcg atatccaaag aaacaattct cccttgactt
     3601 gtgacaatcg cttgagcctt catagcgaga aatatctatc ctatcttcta gaatgtcttc
     3661 caaacgagga aactctcgta aacaaagagg ttttagaggc ctatttaccg tggactaaag
     3721 ttgtacaaga aaagtgcaaa taagaaatct ccagattagg aactatccgt gagttctcta
     3781 gtctggagat ttt
//
LOCUS       DAVLVP010000127         3581 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.127, whole genome shotgun sequence.
ACCESSION   DAVLVP010000127 DAVLVP010000000
VERSION     DAVLVP010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3581)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3581)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3581
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.127"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(88..1503)
                     /locus_tag="VYN69_001912"
     CDS             complement(88..1503)
                     /locus_tag="VYN69_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000151865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEV1534124.1"
                     /translation="MTIFPDDFLWGGAVAANQVEGAYNEDGKGLSVQDVLPKGGLGEA
                     TENPTEDNLKLLGIDFYHKYKEDISLFSEMGFNVFRTSIAWSRIFPKGDEEEPNEAGL
                     KYYDELFDELHAHGIEPLVTLSHYETPLYLARKYHGWIDRRMIHFYEKFARTVLERYK
                     DKVKYWLTFNEVNSVLELPFTSGGIDIPKENLSKQELYQAIHHELVASSLVTKIAREI
                     NSEFKVGCMVLAMPAYPMTPNPKDVWATHEYENLNYLFSDVHVRGYYPNYAKRYFKEH
                     DINIEFAAEDAELLKNYTVDFLSFSYYMSVTQSALPTQYNSGEGNIIGGLVNPYLESS
                     EWGWQIDPIGLRIILNRYYDRYQIPLFIVENGLGAKDQLIKDEFNNLTVQDDYRIQYM
                     KEHLLQVAEALQDGVEIMGYTSWGCIDCVSMSTAQLSKRYGLIYVDRNDDGNGTFNRY
                     KKMSFTWYKGVIESNGESLFK"
     gene            complement(1516..3354)
                     /locus_tag="VYN69_001913"
     CDS             complement(1516..3354)
                     /locus_tag="VYN69_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000120564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEV1534125.1"
                     /translation="MSYKDTVQKILDVIGGEKNVNRVTHCVTRLRLELKDENLVNDDD
                     VKKIPGVIGIMKKNGQYQIILGNDVANYYKEFVKLGNFESDSVVQGHKGNILERIIEY
                     IAGSMTPIIPAMLGGGMLKVLVIILPMLGILQSDSQTIAFLTFFGDAPYYFLPLLLAY
                     SASQKLKVTSTLAMSVAGVLLHPNFVQMVQSGNPLSLFGAPVTPASYGSSVVPILIMV
                     WLMKYIEKIIAKLTLAITKSFLQPTLVLLVSSCIALVVVGPIGVIVGEGLSNLVGQMY
                     GVAGWLTLAILGAIMPFIVMTGMHWAFAPIFLAASIATPDVLILPAMLGSNLAQGAAS
                     MAVALKSKNNNTKQIAFAAGFSALLAGITEPALYGVTLKYKKPLYAAMIGGGLAGLFA
                     GLTSVKAYLFAVPSLIALPQFIYSDVPSNIVNALIVAVISVVITFVLAYIFGIDEEES
                     SSNLEVEAGVSNKKMIFSPISGEIIPLSDVQDKTFSDKLIGDGVAIIPSEGKVYAPFD
                     GKITNIFPTKHAIGLKSDEGVELLIHIGLDTVELKGQGFISHVEEGDRVFKNQLIFEM
                     DLNLIKTKGYETVTPVIVTNTNDFLDVLVLPNNQTIEHSKELLVIL"
     gene            complement(3372..>3581)
                     /locus_tag="VYN69_001914"
     CDS             complement(3372..>3581)
                     /locus_tag="VYN69_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006270038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEV1534126.1"
                     /translation="FAHRLVENTTYCDDDDEDLLALMKNKYPREYECGEQVAMFIQTE
                     YNYLLTSSELVYLMAHIRRLTKNLD"
BASE COUNT         1195 a          729 c          482 g         1175 t
ORIGIN      
        1 agaatttgat gtcttccaaa cctctttatt tcatattcaa tgagatgttg ccttaaccgt
       61 gataatatct aatcatcaat aaacaaacta tttgaataag gattctccat ttgattcaat
      121 cactccttta taccaagtaa aggacatttt cttatatcga ttgaatgtgc catttccatc
      181 atcatttcga tcaacataaa tgagaccgta cctcttagaa agctgtgcag tggacataga
      241 aacgcagtca atacatcccc aagacgtata gcccataatt tccacaccat cttgtagagc
      301 ttcagctact tgcaataaat gttctttcat atactgaatt ctataatcat cttggactgt
      361 taagttatta aattcatctt ttattagttg atctttagca cctaatccgt tttccactat
      421 aaataatgga atttgataac ggtcataata tctatttaaa attatacgta gtccaattgg
      481 atcaatttgc catccccact cagaagactc taaataagga tttactaagc caccaataat
      541 attcccttct cctgaattat actgtgttgg aagagcagat tgagtcacac tcatgtaata
      601 gctaaaggat aaaaaatcta cggtataatt ttttaataac tctgcatctt cagctgcaaa
      661 ctctatgtta atgtcatgtt ccttaaaata tctttttgca taattcggat aataacctct
      721 aacatgcaca tctgaaaata gataatttag attctcatac tcatgagtcg cccatacatc
      781 ttttggattt ggagtcattg gataagctgg catagctaat accatacacc ccaccttaaa
      841 ctctgaatta atctcacgag caatttttgt aaccaaactc gaggcgacta attcatgatg
      901 tatagcttga tataattctt gtttcgaaag attctcctta ggtatatcta ttcctccact
      961 agtaaacggt aattccaaaa cagagtttac ttcgttaaat gtaagccaat atttaacttt
     1021 atctttatac ctttctaaaa ctgttcgagc aaatttttca taaaaatgaa tcattctcct
     1081 atcaatccat ccatgatatt ttcttgctaa atataatgga gtctcatagt gtgaaagagt
     1141 tacaagtggt tctatcccgt gagcatgtag ttcatcaaat aattcatcat aatatttcaa
     1201 accagcttcg ttaggttctt cctcatctcc ttttggaaaa attctactcc atgcaataga
     1261 agtacgaaaa acattaaagc ccatttcaga aaacaaggat atatcttcct tatatttatg
     1321 ataaaaatca atacctaaca attttaagtt atcttctgta ggattttctg ttgcttctcc
     1381 taatccacct ttgggtaaca catcctgaac tgataagccc ttaccatctt cattatatgc
     1441 tccctctact tgattagctg caacagctcc accccaaaga aaatcatctg gaaaaatggt
     1501 cataactttc ctccattata atattaccag taattcctta gaatgctcga ttgtctgatt
     1561 attaggtaat actaatacat ctagaaaatc attggtattc gttacaatta ctggtgtaac
     1621 tgtttcgtag cctttagtct tgattaaatt caagtccatt tcaaaaatca actgattttt
     1681 gaaaactctg tctccttctt ctacatgact aataaaacct tgacctttta gctcaacagt
     1741 atctaatcca atatgaatta gtaactcaac accctcatca ctcttcaatc caattgcgtg
     1801 cttagtcgga aaaatatttg taattttccc atcaaatggt gcataaacct taccttcact
     1861 tgggataatc gctactccgt ctccaattag tttatctgaa aatgttttat cctggacatc
     1921 gcttaacgga atgatttctc ctgatatagg agaaaatatc atttttttat ttgaaactcc
     1981 agcttcaact tctaaattgc tagaactctc ttcttcatcg attccaaata tataagctaa
     2041 tacaaaggta ataacaaccg aaatgaccgc cacaattaaa gcatttacaa tatttgatgg
     2101 cacatcagaa taaataaatt gaggcaacgc tatcaaagat gggacagcaa atagatatgc
     2161 tttaacacta gtaagacctg caaataatcc cgctaatcca ccaccaatca tagctgcata
     2221 aagcggtttt ttatatttta aagtcacacc atataatgca ggttcggtaa tccctgcaag
     2281 taaggctgag aaacctgctg caaaagcaat ttgttttgta ttattatttt tactctttaa
     2341 tgcaacagcc atcgaagcag ccccttgagc taagtttgac cctaacattg ctggaagaat
     2401 taatacgtct ggagtagcaa tagatgccgc caaaaaaata ggtgcaaaag cccaatgcat
     2461 tccagtcata acaataaatg gcataatagc accaagaata gctaatgtaa gccatccagc
     2521 tacaccatac atttgcccaa ctagatttga taatccttca ccaacaatta ctccaatagg
     2581 tccgactaca actaaggcaa tacagcttga tactaataat actagcgtag gttgcaaaaa
     2641 actcttagta atagctagtg ttaatttagc aattattttt tcaatatatt tcatcaacca
     2701 aaccataata agaattggaa cgactgatga accataacta gctggtgtca caggtgcacc
     2761 aaataaacta agaggattcc ctgattgcac catttgaaca aaatttggat ggagaagtac
     2821 acctgctaca gacatagcta atgtagatgt tacttttaat ttttgtgatg cagaataagc
     2881 taataacagc ggtaagaaat aatatggagc atccccaaaa aatgtcaaaa aagcaatagt
     2941 ctgagaatct gattgcaata taccaagcat tggtaaaatg attaccaaga ctttcaacat
     3001 acctccccct aacattgctg gaatgattgg agtcatggaa ccagcgatat actcaatgat
     3061 tctttctaaa atattccctt tgtgcccttg aacaactgaa tcggattcaa aattgccaag
     3121 tttaacgaat tctttataat aattagctac atcattacca agtataattt gatattgtcc
     3181 attctttttc ataataccta ttacacctgg tatcttcttc acatcatcat cattgactaa
     3241 attttcatct tttaattcta atcttaaacg tgttacacaa tgggtaactc tattgacatt
     3301 tttttcacct ccaattacat cgaggatttt ttgtaccgta tctttataac tcatggtatt
     3361 ctcctattct attaatctaa attttttgtt aagcgacgaa tatgagccat caaataaact
     3421 aattcactag aagtcagcaa ataattgtac tccgtttgta taaacattgc cacctgttca
     3481 ccacattcat attctctagg atatttattt ttcattaatg ctaacaagtc ttcatcatca
     3541 tcgtcacaat aagttgtatt ttcaaccaag cgatgggcaa a
//
LOCUS       DAVLVP010000128         3513 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.128, whole genome shotgun sequence.
ACCESSION   DAVLVP010000128 DAVLVP010000000
VERSION     DAVLVP010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.128"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..980
                     /locus_tag="VYN69_001915"
     CDS             <1..980
                     /locus_tag="VYN69_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1534127.1"
                     /translation="GDFVAIDNLNLDIYEGEFFTFLGPSGCGKSTTLRALVGFLDPSS
                     GSIEVNGTDVTHLEPEKRGIGIVFQSYALFPTMTVFDNIAFGLKVKKVAPDVIKAKVS
                     AVAAKIKISDQQLQRNVSELSGGQQQRVALARALVLEPKILCLDEPLSNLDAKLRVDL
                     RKELKRLQKELGITTLYVTHDQEEALTLSDRIAVFNNGYIEQVGTPVEIYHNSQTEFV
                     CDFIGDINVLTDETVHEVLLKNTSVFLEDKKGYIRLEKVRFNRETEQDFILKGTIIDV
                     EFSGVTIHYTIKVSESQILNVTSIDSQAAIRSVGESVELFITPSDVLQF"
     gene            992..2683
                     /locus_tag="VYN69_001916"
     CDS             992..2683
                     /locus_tag="VYN69_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1534128.1"
                     /translation="MRHKLNLKDWLIRLGLIWFLVTFIIYPNFDLVVNVFVKGGEFSL
                     DAVHRVLKSQRALQSIMNSFKLAFSLIITVNVVGILCVLFTEYFDIKGAKILKLGYMT
                     SLIYGGVVLATGYKFVYGPYGLITKFLQNVIPSLDSNWFIGYGAVLFIMTFSGTANHT
                     LFLTNTIRSVDYHTIEAARNMGAKPFTVFRKVVLPTLIPTLFALTIMVFLSGLSAVAA
                     PMIVGGKEFQTINPMIITFAGMGNSRDLAALLAIILGIATTILLTIMNKIEKGGNYIS
                     ISKTKAPLKKQKIASKPWNIIAHIVAYGLFTVFMLPLIFIVLYSFTDPVAIQTGNLTL
                     SNFTLENYRLFFSNSAAFSPFLVSFIYSIIAATTATILAVVFARVVRKHKSRFDFLFE
                     YGALLPWLLPSTLLAVSLLFTFNQPQFLVLNQILVGSLVILLIAYIVVKIPFSYRMVR
                     AILFSVDDEMEDAARSMGASPFYTMMKVIIPFILPVVLSVIALNFNSLLTDFDLSVFL
                     YHPLAQPLGITIRSAGDETATSNAQALVFVYTIVLMIISGTVLYFTQRPGRKVRK"
     gene            2686..3396
                     /locus_tag="VYN69_001917"
     CDS             2686..3396
                     /locus_tag="VYN69_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000127555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEV1534129.1"
                     /translation="MTATSLGLSNIEIVVRIVLSVVIGSIIGLERGSKSQPAGIRTYS
                     IVCLAACLIMMTNEYVSYKFGTGDPTRLGAQVISGVGFLGAGTILITDKKKITGLTTA
                     AGIWASAGIGLAIGVGFYEGALLVAISVWGVISMFQPLKKYLQNRSKMIELYIVVKST
                     EAYNRVLVYCAENGIRMTDSRTAFGDVNSDRIEYFDVPDKKIASFITLELSGRFEHLR
                     LMEEIANIVGVIYVEEIS"
BASE COUNT         1075 a          512 c          666 g         1260 t
ORIGIN      
        1 atggtgattt tgttgcaatt gataatctga atttagatat atatgaaggg gaattcttta
       61 catttcttgg gccttcagga tgtggtaaat caactacttt gagagcattg gtaggttttt
      121 tagatccatc atcaggaagt attgaagtta atggaacaga tgtcactcat ttggaacctg
      181 aaaagcgtgg aattggtatt gtatttcaat cttatgcgct atttccaact atgactgttt
      241 ttgataatat tgcatttggt ttaaaagtta agaaggtagc tccagatgtt attaaagcta
      301 aagtatcagc agtggcagca aaaattaaga tctctgatca acagttacag cgtaatgtat
      361 cagaattatc tgggggtcaa caacaaaggg tagcattggc tcgtgctctg gttcttgaac
      421 ctaaaattct ttgtctagat gaaccattgt caaaccttga cgcaaaatta cgtgtagatt
      481 tgagaaaaga gttgaaaaga cttcaaaaag agttaggtat tactacttta tatgttactc
      541 atgatcaaga ggaagccttg actttatctg atagaattgc agtctttaac aatggataca
      601 tcgaacaggt cggtacacca gtagagattt atcataattc tcaaactgaa tttgtatgtg
      661 atttcattgg agatattaat gttttgaccg atgaaacagt ccacgaagta ttattgaaaa
      721 atacaagcgt tttcttagag gataaaaaag gatacattcg attagagaaa gttcgattca
      781 atcgtgaaac tgaacaagat tttattctaa aagggacaat tattgatgtt gagttttctg
      841 gagttacaat tcactataca ataaaagttt ctgaaagtca gattcttaat gtaacaagta
      901 ttgatagtca ggctgctatt agatctgtcg gagaaagtgt ggaattattt atcacaccat
      961 cagacgttct gcaattttaa ggaggtgcag tatgcgtcat aaattaaatt taaaagattg
     1021 gcttattcgt ttagggttaa tctggttctt agtaacattt attatttatc caaactttga
     1081 tctagtagtg aatgtatttg taaaaggagg agaattttcc cttgatgctg tacatcgtgt
     1141 tctaaaatct cagagggcac ttcagagtat tatgaacagt tttaagttag cattttcact
     1201 cattattaca gttaatgtcg taggtattct ttgtgttcta tttacagagt actttgatat
     1261 taaaggtgct aaaattttaa aattaggtta tatgacctct ttaatttatg gaggagtggt
     1321 tttagcgact ggatataaat ttgtctatgg tccttatgga ttgattacaa aatttttaca
     1381 aaatgttatc ccttctttag actctaactg gtttattggg tatggtgcag tcttattcat
     1441 tatgacattt tcaggaactg ctaatcatac attgttttta acaaatacaa ttcgaagcgt
     1501 tgactatcac actattgagg ctgctcgaaa tatgggagca aaaccattta ctgttttccg
     1561 aaaagtagtg ttaccaacct taattccaac tctatttgca cttactatta tggtttttct
     1621 tagtggttta tctgcagtag cagcacccat gattgttggt ggtaaagaat ttcaaactat
     1681 aaatccaatg attattacat ttgcagggat ggggaattct cgtgatttag ctgccttact
     1741 tgcaattatt ttaggtattg caactacaat tttgcttact atcatgaata agatagaaaa
     1801 aggtggaaat tatatttcta tctctaagac taaagcgcct cttaaaaaac aaaaaattgc
     1861 gtctaagcct tggaatatca ttgctcacat tgtagcatat ggattgttca cagttttcat
     1921 gcttccacta atttttatag tattatactc atttacagat ccagttgcaa ttcaaacagg
     1981 taacttaaca ttatcaaact ttactttaga aaattatcgc ttattcttta gtaatagtgc
     2041 ggcattctct ccattcttgg tcagctttat ttattctatt attgctgcga caacagcaac
     2101 aattctcgca gttgtatttg ctcgtgttgt cagaaaacat aaatctcgtt ttgatttctt
     2161 atttgaatat ggtgctctac ttccttggtt actaccaagt acacttttag cagtaagttt
     2221 attatttact tttaatcagc cacaatttct tgtcttgaat cagattttgg taggtagttt
     2281 ggtaattcta cttattgcat atatagttgt aaaaatccca ttttcttata gaatggtacg
     2341 tgctatttta tttagtgttg atgatgagat ggaagatgca gcaagaagta tgggtgcttc
     2401 acctttttat actatgatga aggttatcat tccatttatt ttaccggttg ttctctctgt
     2461 tattgcttta aactttaact ctttattaac tgacttcgac ttatctgtat tcctttacca
     2521 tcccctagct caaccattag gtattacgat tcgatctgca ggtgatgaaa cagcaacatc
     2581 taatgcacaa gctctggtat ttgtttatac aattgttctg atgattattt ctggaacggt
     2641 attatacttc acacaaagac cggggcgtaa agtaaggaaa taatcatgac agccactagt
     2701 cttgggttat caaatattga aatagttgtc aggattgttt tatcagtagt cattggtagt
     2761 ataattggtt tagagagagg gagcaaatcc cagcctgcag gcatccgaac ttatagtatt
     2821 gtttgtctag ctgcatgttt gattatgatg acgaatgaat acgtatctta taaatttggg
     2881 acaggagatc ctacacgatt aggagctcaa gttatatcag gtgtgggttt tctaggcgct
     2941 ggaacgattc ttattacaga taaaaagaaa attacaggtc tgacaactgc agcaggcatt
     3001 tgggcttcgg caggaattgg attagctatt ggagtaggtt tttatgaggg agctctttta
     3061 gtagccattt ctgtttgggg tgtgatatcc atgttccaac cactaaaaaa atatctacaa
     3121 aatcgttcta aaatgattga attgtatata gtagttaaat ctacagaagc ctataatcgt
     3181 gtattggttt attgtgcaga aaatggtatc agaatgaccg attcaagaac agcgtttgga
     3241 gatgttaatt ctgacagaat tgaatacttt gatgttccag ataaaaaaat agcgtcattt
     3301 attactctgg aattatcagg tagatttgaa catcttcgac tgatggaaga aattgctaat
     3361 attgtaggtg ttatttatgt cgaagaaatt agctgaaatt tagtaaacaa agggacagat
     3421 gtttttgtcc ttttgttttt tagatgtttt aatcatctaa tttttgagta gatttttgct
     3481 cagaggtttt tatgaaagat tataaaatca att
//
LOCUS       DAVLVP010000129         3508 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.129, whole genome shotgun sequence.
ACCESSION   DAVLVP010000129 DAVLVP010000000
VERSION     DAVLVP010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3508
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.129"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(31..1581)
                     /locus_tag="VYN69_001918"
     CDS             complement(31..1581)
                     /locus_tag="VYN69_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000392528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HEV1534130.1"
                     /translation="MEEQSEIVRSKKEFAFASSTILSQVGRGIIVGLIVGIIVGSFRF
                     LIEKGFHMIQGVYQDQGYLVRNLFVLVLFYILICWLSAKLTRSEKDIKGSGIPQVEAE
                     LKGLMSLNWWGILWKKYVLGILAIASGLMLGREGPSIQLGAVGGKGIAKWLKSSPVEE
                     RSLIASGAAAGLAAAFNAPIAALLFVVEEVYHHFSRFFWVSTLAASIVANFVSLLMFG
                     LTPVLDMPDNIPPMTLDQYWIYLVMGIFLGFSGFLYEKAVLNVGRVYDLIGQKIHLDR
                     AYYPILAFILIIPVGIFLPQIIGGGNQLVLSLTEQNFSFQVLLAYFLIRFIWSMISYG
                     SGLPGGIFLPILALGSLLGALVGVICVNLGLVSQEQFPIFVILGMSGYFGAISKAPLT
                     AMILVTEMVGDIRNLMPLGLVTLVSYIIMDLLKGTPVYEAMLEKMLPEEVSSEGEVTL
                     IEIPVSDKIAGKQVHELNLPHNVLITTQVHNGKSQTVNGSTRMYLGDMIHLVIPKSEI
                     GKVKDLLL"
     gene            complement(1597..2376)
                     /locus_tag="VYN69_001919"
     CDS             complement(1597..2376)
                     /locus_tag="VYN69_001919"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEV1534131.1"
                     /translation="MATIKEIKELLVTVKELESPIFLELEKDNRSGVQKEISKRKRAI
                     QAELDENLRLESMLSYEKELYKQGLTLIAGIDEVGRGPLAGPVVAAAVILPKNCKIKG
                     LNDSKKIPKKKHLEIFQAVQDQALSIGIGIIDNQVIDQVNIYEATKLAMQEAISQLSP
                     QPEHLLIDAMKLDLPISQTSIIKGDANSLSIAAASIVAKVTRDELMKEYDQQFPGYDF
                     ATNAGYGTAKHLEGLTKLGVTPIHRTSFEPVKSLVLGKKES"
     gene            complement(2363..3214)
                     /gene="ylqF"
                     /locus_tag="VYN69_001920"
     CDS             complement(2363..3214)
                     /gene="ylqF"
                     /locus_tag="VYN69_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000201313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEV1534132.1"
                     /translation="MATIQWFPGHMSKARRQVQENLKFVDFVTILVDARLPLSSQNPM
                     LTKIVGDKPKLLILNKADLADPAMTKEWRQYFESQGIQTLAINSKEQVTVKVVTDAAK
                     KLMADKIARQKERGIQIETLRTMIIGIPNAGKSTLMNRLAGKKIAVVGNKPGVTKGQQ
                     WLKTNKDLEILDTPGILWPKFEDETVALKLALTGAIKDQLLPMDEVTIFGINYFKEHY
                     PEKLAERFKQMKIEEEPSVIIMDMTRALGFRDDYDRFYSLFVKEVRDGKLGNYTLDTL
                     EDLDGND"
BASE COUNT         1030 a          741 c          685 g         1052 t
ORIGIN      
        1 tatataacat aaattatgta aatactcatg ctacaacaac aaatctttga cttttccaat
       61 ttcacttttt ggaataacca ggtgaatcat atcacccaga tacattctgg ttgagccgtt
      121 aactgtttgg ctcttgccat tatggacttg agttgtgatg aggacgttgt gtggtaagtt
      181 gagttcatga acttgtttcc cagcaatttt atcagaaact ggtatttcga taagtgtaac
      241 ttctccttcg ctagatactt cttctggaag cattttttcc agcatggctt catagactgg
      301 cgtacctttg agcaaatcca tgataatata agaaacaaga gtgacaagac caagtggcat
      361 gaggttgcga atatctccta ccatctcagt tacgaggatc atagcggtta agggagcttt
      421 tgatatggct ccaaaatagc cactcattcc tagaatgaca aatataggga attgctcttg
      481 actgacaagt ccaagattga cacagataac accaactaag gcaccaagca aagaaccaag
      541 agctaaaatg gggaggaaaa ttcctcctgg cagtccactt ccatagctaa tcatactcca
      601 aataaagcgg attaaaaagt aagctaataa aacttggaaa ctaaaatttt gttcagttaa
      661 agaaaggaca agctgatttc cgccaccaat gatttgaggt aagaagattc cgactggtat
      721 gataaggata aaagccaaga tgggataata agccctatcc aaatggattt tttgaccaat
      781 caagtcataa actcttccaa cgtttaatac agctttctca tagagaaaac ctgaaaatcc
      841 aaggaaaatt cccatgacga gatatatcca atactggtct agggtcatgg gaggaatgtt
      901 atctggcata tccaatactg gtgtcaaacc gaacatgagt agagacacaa agtttgctac
      961 gatgctggct gctagagttg agacccagaa aaagcgcgaa aaatggtgat agacttcttc
     1021 tacaacaaag agaagtgctg caataggagc attaaaggct gcggctaaac ctgctgcagc
     1081 tccactggca atcaaggaac gttcctctac tggactggat ttgagccact tggcaattcc
     1141 tttaccacca actgctccaa gttgaatact gggtccctct cgacccagca tgagtccact
     1201 ggcaatagcg agaataccta gcacatattt tttccaaaga atgccccacc agttgaggga
     1261 catgaggcct ttcagttcgg cttcgacttg aggaattcct gagcctttaa tatctttttc
     1321 tgaccgtgtt agtttggcac tgagccaaca gatgagtata taaaacaaaa ccagtacaaa
     1381 aagattgcgc actaagtacc cttgatcttg ataaactcct tgtatcatgt ggaagccctt
     1441 ttcaattaag aaacgaaagg atccgacgat aattccaacg atgaggccga caatgattcc
     1501 tcgaccaact tgggatagta tagtgctgga tgcaaaggcg aattctttct tagaacggac
     1561 tatttccgac tgttcctcca tggtatttcc tttcaattaa ctttcttttt tacctaaaac
     1621 cagtgattta acgggttcaa agctggttcg gtgaattggg gtaactccta gttttgtgag
     1681 gccttccaga tgtttagctg tgccatatcc tgcattagta gcgaaatcat agccagggaa
     1741 ctgctgatcg tattctttca tcaattcatc acgtgttacc ttggctacta tagatgctgc
     1801 tgcgatagag agggagttgg catctccttt gataatggag gtttgtgaaa tgggcaagtc
     1861 cagtttcatg gcatcaatca aaaggtgctc tggttgaggg ctgagctggg agattgcttc
     1921 ttgcatggct agtttggttg cttcatagat gttgacttgg tcgatgacct gattatctat
     1981 gataccaatt ccaatcgaca aggcttggtc ttgaacggct tggaaaatct ccagatgttt
     2041 ctttttagga attttcttgc tgtcgttgag acctttaatc ttacaatttt taggtaaaat
     2101 aacggccgca gcgactacag gaccagcaag aggaccacga ccaacctcat caatacctgc
     2161 aattaaggtc aatccttgct tataaagttc tttttcataa gaaagcatgg attccaagcg
     2221 caaattttca tctaattcag cttggatggc tcttttacgc ttgctgattt ccttttgaac
     2281 tcctgagcga ttatcctttt caagctctaa aaaaataggg ctttctaact ccttgactgt
     2341 cacaaggagt tctttgattt ctttaatcgt tgccatcgag gtcttccaat gtatctaagg
     2401 tatagttacc gagtttgcca tcacggactt ccttcacgaa gagactgtaa aaacggtcat
     2461 agtcatcacg gaaaccgagg gcgcgggtca tatccataat aatcacagac ggttcttctt
     2521 caattttcat ttgtttgaag cgttcagcca gcttttctgg ataatgttct ttgaaataat
     2581 tgataccaaa aatggtaacc tcatccatag gaagcaactg gtctttgata gctccagtca
     2641 atgccaactt aagtgcaaca gtttcatcct caaacttagg ccagagaatc cccggtgtat
     2701 ccaagatttc caggtcttta ttggttttaa gccattgttg accttttgtg acccctggct
     2761 tgtttccaac aacagcaatc tttttaccag ccaaacggtt catcagagtt gatttaccag
     2821 cgtttggaat cccgataatc atggtacgca aggtttcaat ctgaatccca cgttctttct
     2881 ggcgagcaat cttatcagcc atgagcttct tggccgcatc tgttacaact tttacagtca
     2941 cttgctcttt ggagttgata gctagcgtct ggattccttg tgattcaaaa tactgacgcc
     3001 attccttggt cattgctgga tcagccaagt cggccttgtt taaaatcaag agttttggtt
     3061 tatcaccaac aatcttggtc aacataggat tttgactaga tagaggcaag cgtgcatcta
     3121 ctaaaatcgt cacaaaatca acaaatttta aattctcctg cacctgtcga cgagctttag
     3181 acatgtgacc aggaaaccat tgaatagtag ccattgagtt ttttcctttc gtagctctga
     3241 agctgttaat ctagaactaa gagatctcaa tctaaaaatg aatgatgtcc aaacagttct
     3301 tgttttatga cataaaaatc tactttatat tatatcatgc cttctttatt ttgtccgatg
     3361 atagcctttt accctatata gaaaaaagcc atcaaatgat gacttttcag agggagcaaa
     3421 aaactgtcct ctttatgttt taaacattta acccaaatca ttcatacctc tctcaactag
     3481 atgtaactta caaaacccct gacctcat
//
LOCUS       DAVLVP010000130         3460 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.130, whole genome shotgun sequence.
ACCESSION   DAVLVP010000130 DAVLVP010000000
VERSION     DAVLVP010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.130"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            51..>3460
                     /locus_tag="VYN69_001921"
     CDS             51..>3460
                     /locus_tag="VYN69_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="G5 domain-containing protein"
                     /protein_id="HEV1534133.1"
                     /translation="MEKYFGEKQERFSFRKLSVGLVSATISSLFFMSVLASSSVDAQE
                     TAGVHYKYVADSELSSEEKKQLVYDIPTYVENDDETYYLVYKLNSQNQLAELPNTGSK
                     NERQALVAGASLAALGILIFAVSKKKVKNKTVLHLVLVAGIGNGVLVSVHALENHLLL
                     NYNTDYELTSGEKLPLPKEISGYTYIGYIKEGKTTSDFEVSNQEKSAATPTKQQKVDY
                     NVTPNFVDHPSTVQAIQEQTPVSSTKPTEVQVVEKPFSTELINPRKEEKQSSDSQEQL
                     AEHKNLETKKEEKISPKEKTGVNTLNPQDEVLSGQLNKPELLYREETIETKIDFQEEI
                     QENPDLAEGTVRVKQEGKLGKKVEIVRIFSVNKEEVSREIVSTSTTAPIPRIVEKGTK
                     KTQVIKEQPETGVEHKDVQSGAIVEPAIQPELPEAVVSDKGVPEVQPALPEAVVTDKG
                     EPAVQPELPEAVVTDKGEPEVQPELPEAVVTDKGEPEVQPALPEAVVSDKGEPEQVAP
                     LPEYTRPQAGAVVEPAIQPELPEAVVTDKGEPAVQPELPEAVVTDKGETEVQPESPDT
                     VVSDKGEPKQVAPLPEYTGPQASAIVEPEQVAPLPEYTGVQAGSIVEPEKVEAPKEYT
                     GKIEQPSAEDTKPENEASSTNGESERPKDKIKEEKQVDKKLELRNVSNVELYTVENNK
                     YRHITAVDGALDSSLKYFMKVKSENFKDIMLPVTKIESTTKNNKEVYKIVAHAENLIQ
                     HENNVISNDYTYYLPKTQQSETGVYTSFKNLVDAMNSDPNGTFHLGATMDAREVELPD
                     DQESYVKNEFYGKLIGENNGKYYAIYNLKKPLFKTLNTATIQNLSIKEANVSSKEDAA
                     TISKEAKYNTLIDNVHSDGIIAGERGIGGLVSKVDNSRISNSSFTGRITNTYDTTVGY
                     EIGGLVGKLSGSLASIEKSIASIDIASNAKSGDQIVGGIAGVVEKSATIKYSYVEGNV
                     NNVRHFGKVGGVAGNLWDRDSQDVSKSGKLSYVLSDVNVTNGNAISGDNFDNMKEDHA
                     YSSKGNKVVNVVQVDDELVTKDSDVQRGTVLDADKVKEKKAELVSKHSTKVEDFDFTS
                     RYTTIYNEVTGYQKSREQVYKNIEKLLPFYNRETIVKYGNLVDESSEL"
BASE COUNT         1309 a          532 c          709 g          910 t
ORIGIN      
        1 tataataaag gtctttgtaa catatataaa ttttatttca aggaggaata atggaaaagt
       61 attttggtga aaaacaagag cgtttttcat ttagaaaatt atcagtagga cttgtatctg
      121 caacgatttc aagtttattt tttatgtctg tattagctag ttcatctgtg gatgctcaag
      181 aaactgcggg agttcactat aaatatgtgg cagattcaga gctatcatca gaagaaaaga
      241 agcagcttgt ctatgatatt ccgacatacg tggagaatga tgatgaaact tattatcttg
      301 tttataagtt aaattctcaa aatcaactgg cggaattacc aaatactgga agcaagaatg
      361 agaggcaagc cctagttgct ggtgctagct tagctgctct gggaatttta atttttgctg
      421 tttccaagaa aaaggttaag aataaaacgg tattacattt agtattggtt gcgggaatag
      481 gaaatggtgt cttagtttca gtccatgctt tagaaaatca tcttttgcta aattacaata
      541 cggactatga attgacctct ggagaaaaat tacctcttcc taaagagatt tcaggttaca
      601 cttatattgg atatatcaaa gagggaaaaa cgacttctga ttttgaagta agtaatcaag
      661 aaaaatcagc agccactcct acaaaacaac aaaaggtgga ttataatgtt acaccaaatt
      721 ttgtagacca tccatcaaca gtacaagcta ttcaggaaca aacacctgtt tcttcaacta
      781 agccgacaga agttcaagta gttgaaaaac ctttctctac tgaattaatc aatccaagaa
      841 aagaagagaa acaatcttca gattctcaag aacaattagc cgaacataag aatctagaaa
      901 cgaagaaaga ggagaagatt tctccaaaag aaaagactgg ggtaaataca ttaaatccac
      961 aggatgaagt tttatcaggt caattgaaca aacctgaact cttatatcgt gaggaaacta
     1021 tagagacaaa aatagatttt caagaagaaa ttcaagaaaa tcctgattta gctgaaggaa
     1081 ctgtaagagt aaaacaagaa ggtaaattag gtaagaaagt tgaaatcgtc agaatattct
     1141 ctgtaaacaa ggaagaagtt tcgcgagaaa ttgtttcgac ttcaacgact gcgcctattc
     1201 caagaatagt cgaaaaaggt actaaaaaaa ctcaagttat aaaggaacaa cctgagactg
     1261 gtgtagaaca taaggacgta cagtctggag ctattgttga acccgcaatt cagcctgagt
     1321 tgccagaagc tgtagtaagt gataaaggcg taccagaagt tcaaccagcg ttaccggaag
     1381 cagtcgtaac cgacaaaggt gagcccgctg tccaacctga attacccgaa gctgttgtaa
     1441 ctgacaaagg cgagcctgaa gttcaacctg aattacccga agctgttgta actgacaaag
     1501 gcgagcctga agttcaacca gcgttacccg aagcggttgt aagtgataaa ggtgaaccag
     1561 agcaggtagc cccattgcca gaatatacaa gaccacaagc aggtgcagta gttgaacctg
     1621 caatccaacc agagttacca gaagctgttg taactgacaa aggagaacca gcagtccaac
     1681 cagagctacc ggaagcggtt gtaactgaca aaggtgagac tgaggttcaa ccagagtcgc
     1741 cagatactgt ggtaagtgat aaaggtgaac ctaaacaggt agcaccgctt ccagaatata
     1801 caggaccaca agccagcgct attgttgaac cagagcaagt agcaccacta ccagaataca
     1861 caggggtaca agccggatcg atagtcgagc cagagaaagt agaagcaccg aaagaatata
     1921 ctggtaaaat cgaacaacct agtgcagaag atactaaacc tgaaaatgag gcaagctcta
     1981 caaatgggga atcggaacgt cctaaagata aaattaaaga agaaaaacaa gtcgacaaga
     2041 aactagaact tagaaatgtt tcaaacgtgg aattatatac ggtagaaaat aataaatatc
     2101 gacatattac ggctgtagat ggtgctttag atagttcatt gaagtatttc atgaaggtaa
     2161 aatcagaaaa ttttaaagat ataatgttgc ctgttactaa aattgaaagt acaacaaaaa
     2221 acaataaaga agtatacaaa attgtggctc atgcagaaaa tctaattcaa cacgaaaaca
     2281 atgttatttc gaatgactat acatattatt taccaaaaac tcagcaaagt gaaactggcg
     2341 tctatacatc atttaaaaat ttagttgatg caatgaatag tgaccctaat ggaacattcc
     2401 atttaggtgc aactatggat gctcgtgaag ttgaacttcc ggatgatcaa gaaagttatg
     2461 taaaaaatga attttacgga aaattaatag gtgaaaataa tggtaagtat tatgctattt
     2521 ataacttgaa aaaaccttta tttaaaactc taaacaccgc tactattcag aatctttcaa
     2581 taaaagaagc gaatgtttct tctaaagaag atgctgctac aatatcgaaa gaagcaaaat
     2641 acaatactct tatagataat gttcattctg atggtattat agctggtgaa cgtggaattg
     2701 gtggattggt ttcaaaagta gataactcga gaatttctaa tagtagtttt acaggcagaa
     2761 taactaacac atatgataca actgtagggt atgaaattgg tggtttagta ggtaaacttt
     2821 ctggttcgct tgcttctata gagaaatcaa tagcatcgat tgacatagcc tctaatgcaa
     2881 aatcaggaga tcaaattgtt ggtggtatag ccggagttgt tgagaaaagt gcgacaatta
     2941 agtatagcta tgttgaagga aatgttaaca atgtgcgaca ctttggtaaa gttgggggag
     3001 ttgcaggtaa tctttgggat agagattcac aagatgtaag taaatcaggt aaactatcat
     3061 atgttttaag tgatgttaat gtaacaaatg gtaatgctat atcgggtgat aattttgata
     3121 atatgaaaga agatcatgct tatagtagta aaggcaataa agtagtaaat gtagtccaag
     3181 tagatgatga acttgtgact aaagattcag atgtacaaag aggtacggta ttagatgctg
     3241 ataaagtaaa agagaaaaag gcagaattgg tttccaaaca cagcactaaa gtagaagatt
     3301 ttgatttcac ttcaagatac actactattt acaatgaagt aacaggttat caaaagtcga
     3361 gagaacaagt atataaaaat attgaaaaac tattaccgtt ctataatcga gagacaattg
     3421 ttaaatatgg taatttagta gatgaaagca gcgagctatt
//
LOCUS       DAVLVP010000131         3443 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.131, whole genome shotgun sequence.
ACCESSION   DAVLVP010000131 DAVLVP010000000
VERSION     DAVLVP010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.131"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(269..850)
                     /gene="pdxT"
                     /locus_tag="VYN69_001922"
     CDS             complement(269..850)
                     /gene="pdxT"
                     /locus_tag="VYN69_001922"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000689942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HEV1534134.1"
                     /translation="MKIGILALQGAFAEHAKVLDQLGVESVELRNLDDFQQDQSDLSG
                     LILPGGESTTMGKLLRDQDMLIPIREAILSGLPAFGTCAGLILLAKEIASQEESHLGT
                     MDMVVERNAYGRQLGSFYTEAECKGVGQIPMTFIRGPIISSVGEDVEILARVDNQIVA
                     AQEKNMLVTSFHPELTDDVRLHQYFINMCKEKR"
     gene            complement(851..1726)
                     /gene="pdxS"
                     /locus_tag="VYN69_001923"
     CDS             complement(851..1726)
                     /gene="pdxS"
                     /locus_tag="VYN69_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012054698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HEV1534135.1"
                     /translation="MTENRYELNKNLAQMLKGGVIMDVQNPEQARIAEAAGAAAVMAL
                     ERIPADIRAAGGVSRMSDPKMIKEIQEAVSIPVMAKVRIGHFVEAQILEAIEIDYIDE
                     SEVLSPADDRFHVDKKEFQVPFVCGAKDLGEALRRIAEGASMIRTKGEPGTGDIVQAV
                     RHMRMMNQEIRRIQNLREDELYVAAKDLQVPVELVQYVHEHGKLPVVNFAAGGVATPA
                     DAALMMQLGAEGVFVGSGIFKSGDPVKRASAIVKAVTNFRNPQILAQISEDLGEAMVG
                     INENEIQILMAERGK"
     gene            complement(1878..3257)
                     /locus_tag="VYN69_001924"
     CDS             complement(1878..3257)
                     /locus_tag="VYN69_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEV1534136.1"
                     /translation="MSKIVVVGANHAGTACINTMLDNFGNENEIVVFDQNSNISFLGC
                     GMALWIGEQIDGAEGLFYSDKEKLEAKGAKVYMNSPVLSIDYDNKVVTAEVEGKEHKE
                     SYEKLIFATGSTPILPPIEGVEIVKGNREFKATLENVQFVKLYQNAEEVINKLSDKSQ
                     HLDRIAVVGGGYIGVELAEAFERLGKEVVLVDIVDTVLNGYYDKDFTQMMAKNLEDHN
                     IRLALGQTVKAIEGDGKVERLITDKESFDVDMVILAVGFRPNTALADGKIELFRNGAF
                     LVDKKQETSIPGVYAVGDCATVYDNARKDTSYIALASNAVRTGIVGAYNACGHELEGI
                     GVQGSNGISIYGLHMVSTGLTLEKAKAAGYNATETGFNDLQKPEFMKHDNHEVAIKIV
                     FDKDSREILGAQMVSHDIAISMGIHMFSLAIQEHVTIDKLALTDLFFLPHFNKPYNYI
                     TMAALTAEK"
BASE COUNT          998 a          784 c          619 g         1042 t
ORIGIN      
        1 agcgataacg aagatagtag catatgtaaa actctaagca ggcagagcct gcttagagtt
       61 ttacaatcga atttcccaaa gaggaagcaa gatgaaagcc gtcattgctt atgctcataa
      121 gattctcaga atcgtttaca aactactctc caccaagcat acttatcaaa aagaaaagac
      181 gctaggactg aggaaacagt tctaacgccc aaactaaaaa aatttcaatt acagtatagc
      241 acaggaagtg attttttgcg cttttttact atcttttttc tttacacata ttgataaagt
      301 actggtgcaa gcgaacatca tcagtcaatt ctggatgaaa agaagttacc aacatatttt
      361 tttcttgggc tgcaacgatt tgattatcaa ctcttgctag aatttctaca tcctctccaa
      421 cactactaat aatcggacca cggataaagg tcattggaat ctgaccaact cctttacatt
      481 ctgcttccgt atagaaactt cctaattggc gcccataggc attgcgctcg accaccatat
      541 ccatagttcc tagatgactc tcttcctgag aagcaatttc cttagccagc aaaatcaagc
      601 ctgcacaggt tccaaaggct ggtaaaccag atagaatggc ttctcgaatg gggatcagca
      661 tgtcctggtc tcgtaagagc ttccccatgg ttgtagactc accaccaggc aaaatcaaac
      721 ccgacaagtc actctgatct tgctgaaaat catctagatt tctgagttct acactctcga
      781 cacctaattg atctagcact tttgcatgtt ctgcaaaggc cccttgcaag gccaatattc
      841 cgattttcat ctattttcct cgttcagcca tgagaatttg gatttcattt tcattaatac
      901 caaccatggc ttctcctaaa tcttcagaga tttgagctag gatttgagga ttacggaagt
      961 tagtcacagc cttaacaatg gcactcgctc gtttaacagg atctcctgac ttgaaaatac
     1021 ctgaaccgac aaagaccccc tctgccccta gttgcatcat taacgcagca tctgctggcg
     1081 ttgcaacacc tccagcagcg aaatttacaa ctggcaattt tccatgttca tgaacatatt
     1141 ggaccaattc tacagggact tgcaaatcct tggcagcaac ataaagctcg tcctcacgta
     1201 agttttgaat gcggcgaatt tcctgattca tcatacgcat atgacgaaca gcttggacga
     1261 tatcccctgt ccctggttct cctttggtac gaatcatgga agcaccttca gcgatacgac
     1321 gcaaggcttc acccaaatcc ttagcaccac agacaaaagg aacttggaat tctttcttgt
     1381 ccacatggaa acggtcatca gctggagata gaacttcact ctcgtcgata taatcaattt
     1441 caatagcctc taaaatctga gcttcaacaa aatgcccgat tctgacctta gccattactg
     1501 gaatactaac cgcttcttgg atttccttaa tcatctttgg gtcgctcatg cgggaaactc
     1561 ctccagctgc acgaatatca gccggaattc gttccaaggc catcacagct gccgcaccag
     1621 cagcttctgc gatacgagcc tgttcaggat tctgcacatc cataataaca ccacccttga
     1681 gcatctgtgc caagttttta tttagttcat aacgattttc agtcatttgt tttatcctca
     1741 tcatttgtat tggtagctac cttttcattt taagtcagaa cagaatgaag cacaagataa
     1801 aaaaactata attgtatggg gacagattag ctaaaaacta tctgacctta acttaaggcc
     1861 agatagctca ttcattttta tttttcagcc gtaagggcag ccattgtgat gtagttgtat
     1921 ggtttgttga agtgtggcaa gaagaagagg tctgtcaatg ccaatttatc aattgtcaca
     1981 tgctcttgga tagcaagtga gaacatgtgg attcccatgc taattgcaat atcatgggaa
     2041 accatttggg caccaagaat ttcacggcta tctttgtcaa agacaatctt aattgctact
     2101 tcatggttgt catgtttcat gaattctggt ttttgaagat cgttaaagcc tgtttcagtt
     2161 gcgttgtaac cagcagcttt cgctttttca agagtcaaac cagttgaaac catgtgaaga
     2221 ccgtagattg agataccatt tgaaccttga acaccgattc cttccaattc atgtccacaa
     2281 gcgttgtagg caccaacgat accagtgcga acagcatttg aagcaagagc gatatagctt
     2341 gtatctttac gagcattgtc ataaacagtc gcacagtcac caacagcgta aacgcctggg
     2401 atagatgttt cttgtttctt gtctacaagg aaggcaccgt tgcggaagag ttcgatctta
     2461 ccatcagcaa gggctgtgtt tggacggaaa ccaactgcaa ggataaccat atccacgtca
     2521 aagctttctt tgtcagtaat caagcgttca actttaccgt caccttcgat tgctttaaca
     2581 gtttgaccta gagccaagcg gatgttgtga tcttccaagt tcttcgccat catttgtgtg
     2641 aagtctttgt catagtaacc gttcaagaca gtatcaacga tatcaacaag gacaacttct
     2701 tttccaagac gttcaaaggc ctcagcaagt tcaacaccga tgtaaccacc accaacaacg
     2761 gcgatacggt cgaggtgttg gctcttgtca gaaagtttat tgataacttc ttcagcattt
     2821 tggtacaatt tcacgaattg aacattttca agagttgctt taaattcgcg gtttccttta
     2881 acaatttcaa caccttcgat tggtggcaag attggtgtag aacctgtagc gaaaatcaat
     2941 ttctcgtatg attctttgtg ttcttttcct tcaacttccg ctgtaactac tttgttatca
     3001 tagtcgattg aaagaacagg tgagttcatg taaactttag cacctttagc ttccaatttt
     3061 tctttatcag aatagaacaa gccttcagca ccgtcaattt gttcaccaat ccaaagagcc
     3121 attccacatc ctagaaaaga gatgttagag ttttggtcaa atacaacaat ttcgttctca
     3181 tttccaaaat tatccaacat ggtattgata catgctgtac cagcgtggtt agcaccgact
     3241 acaacgattt tactcataaa aaatttcctg ccttcattct aaatttactt tactagtata
     3301 ccatttaccg ttagcgttta caaggggggt actattattt cagcattttt tatcaaaaat
     3361 aattccccat ttatcctgtt ttcaagcctc atgaatttta tgagcatatt tcttgacttt
     3421 ttccaaattt tgtttgtgaa aat
//
LOCUS       DAVLVP010000132         3420 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.132, whole genome shotgun sequence.
ACCESSION   DAVLVP010000132 DAVLVP010000000
VERSION     DAVLVP010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3420
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.132"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(2169..3420)
                     /locus_tag="VYN69_001925"
                     /pseudo
     CDS             complement(2169..3420)
                     /locus_tag="VYN69_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001856522.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="chordopoxvirus G3 family protein"
BASE COUNT          863 a         1125 c          767 g          665 t
ORIGIN      
        1 agtgcttcag cctcagcaag cacctcagcg tctgaatcgg catcaaccag cgcctcagcc
       61 tcagcatcaa caagtgcatc agcttcagca agcacctcag cgtctgaatc agcatcaaca
      121 agtgcctcgg cttcagcatc aacgagtgct tcggcttcag cgtcaaccag tgcatcagag
      181 tcagcaagta ccagtgcgtc agcttcagcg tcaaccagcg cctcagcctc agcaagtacc
      241 agtgcgtcag cttcagcatc aaccagtgcg tcggcttcag cgtcaacaag tgcttcagcc
      301 tcagcaagca cctcagcgtc tgaatctgca tcaacgagtg cttcagcttc agcaagcaca
      361 agtgcgtcag catcagcctc aaccagtgct tcggcttcag catcgacaag tgcctcagct
      421 tccgcatcaa ccagtgcctc agcttcagca agtaccagcg catcagtctc agcaagcacc
      481 agtgcttcag cttcagcatc aacgagtgcg tcagcctcag caagcaccag tgcttcagcc
      541 tcagcatcaa caagtgcttc agtctcagcg tcaaccagtg cctctgaatc cgcatcaaca
      601 agtgcatcgg cttcagcaag caccagtgcg tcggcttcag caagtactag cgcctcagcc
      661 tcagcgtcaa ccagtgcatc agcctcagca agtactagtg catcagcttc agcatcaacg
      721 agtgcatcgg cttcagcgtc aaccagtgca tcagtctcag caagcaccag tgcgtcggct
      781 tcagcatcaa caagtgcatc ggcttcagca agcaccagtg cttcggcttc agcaagcacg
      841 tcagcatcaa caagcacatc agcatctgaa tcagcgtcaa ccagtgcgtc agcctcagca
      901 agtaccagcg cctcagcctc agcatcaacg agtgcgtcag cttccgcatc aacgagtgcg
      961 tctgaatcgg catcaacgag tgcgtcagcc tcagcaagta ccagcgcctc agcctcagca
     1021 tcaacgagtg cgtcggcatc agcaagtaca agtgcatcag cttcagcgtc aaccagtgcg
     1081 tccgcttcag cgtcaaccag tgcgtcagct tccgcatcaa cgagtgcgtc ggcttcagcg
     1141 tcaaccagtg cctcggcttc cgcatcaacg agtgcgtcgg catcagcaag tacaagtgcc
     1201 tcagcctcag catcaacgag tgcgtcagcg tcggcttcag caagtacctc agcgtctgaa
     1261 tcggcatcaa cgagtgcttc agcttcagca tcaacgagtg cgtcagcctc agcaagcacc
     1321 agtgcttcgg cttcagcaag cacgtcagca tcaacaagca caagtacatc ggcaagcacg
     1381 tcagcatcga caagtgcaag cacatcggcg tcaacaagtg caagtacgtc agcatctgaa
     1441 tcggcctcaa ccagtgcgtc gacttcagca agtacaagcg cctcagcctc agcaagtatc
     1501 tcagcgtctg aatcggcatc aaccagcgcc tcagcctcag catcaacaag tgcatcagct
     1561 tcagcaagta ccagtgcgtc ggcttcagca tcaacgagtg cttcagtctc agcgtcaacc
     1621 agtgcctctg aatccgcatc aaccagtgca tcagagtcag caagtactag cgcctcagcc
     1681 tcagcatcaa ccagtgcatc agcctcagca agtatctcag cgtctgaatc ggcatcaacg
     1741 agtgcgtcag cctcagcgtc aaccagcgcc tcagcctcag caagtaccag tacctcagcg
     1801 tctgaatcgg catcaaccag tgcatcagct tcagcatcaa cgagtgcttc ggcttccgca
     1861 tcaacaagtg cttcagcttc cgcatcaaca agtgcttcag cttccgcatc aacgagtgcg
     1921 tcggcttccg catcaacgag tgcgtcagct tccgcatcaa cgagtgcttc ggcttcagcg
     1981 tcaaccagtg cgtcagcctc agcaagcaca agcgcctcag cttcagcaag tactagtgca
     2041 tcagcttcag cgtcaacgag tgcttcggct tcagtatctg caagcacatc agcatctgaa
     2101 tccgcatcaa cgagtgcctc agcctcagca tcaacaagtg catcggcttc agcatcaaca
     2161 agtgcgtctg aatccgcatc aacgagtgct tcggcttcag caagcaccag cgcgtcagct
     2221 tcagcaagca caagcgcctc tgagtcagcg tcaaccagtg cgtcagcttc agcaagtacc
     2281 agtgcgtctg cgtcagcctc agcaagtacc agcgcctcgg cttcagcaag cacaagtgct
     2341 tctgaatccg catcaacaag tgcgtcagcc tcagcatcga caagtgcgtc agcttcagcg
     2401 tcaacaagtg cctcggcttc cgcatcaacc agtgcctcgg cttctgaatc tgcatcaacg
     2461 agcgcctcag cttcagcatc aaccagtgca tcagcttcag caagtaccag tgcgtctgaa
     2521 tccgcatcaa ccagtgcatc agcctcagca agcaccagtg cgtcggcctc agcaagcacc
     2581 agtgcttcgg cttcagcaag cacaagcgcc tcagcttcag catcaacaag tgcctctgaa
     2641 tccgcatcaa caagtgcatc ggcttcagca agcaccagtg cgtcggcttc agcatcaacg
     2701 agtgcatcgg cttcagcgtc aaccagtgca tcagagtcag caagtaccag tgcgtcagct
     2761 tccgcatcaa cgagtgcatc ggcttcagca agcaccagtg cttcggcttc agcaagcaca
     2821 agcgcctcag cttcagcgtc aacgagtgcg tcagcttcag catcaacaag tgcatcagct
     2881 tcagcatcaa ccagtgcatc agcctcagca agtaccagcg cctcagcttc agcatcgaca
     2941 agtgcgtcag cttcagcaag cacatcagct tctgaatccg catcaacgtc tgcctcagca
     3001 agtacatcag cttctgaatc ggcatcaacg agtgcttcgg cttcagcaag tacctcagcg
     3061 tctgaatctg catcaaccag tgcatcagct tcagcaagta ccagtgcttc agtctcagca
     3121 tcaaccagcg cctcggcctc agcgtcaacc agcgcctcag cctcagcaag taccagtacc
     3181 tcagcgtctg aatctgcatc aaccagtgca tcagcttcag catcaacgag tgcatcggct
     3241 tcagcaagca ccagtgcttc ggcttcagca agtactagtg catcagcttc agcgtcaacg
     3301 agtgcttcgg cttcagtatc tgcaagcaca tcagcatctg aatccgcatc aacgagtgcc
     3361 tcagcctcag catcaacaag tgcatcggct tcagcatcaa caagtgcgtc tgaatccgca
//
LOCUS       DAVLVP010000133         3306 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.133, whole genome shotgun sequence.
ACCESSION   DAVLVP010000133 DAVLVP010000000
VERSION     DAVLVP010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3306
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.133"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            338..499
                     /locus_tag="VYN69_001926"
     CDS             338..499
                     /locus_tag="VYN69_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534137.1"
                     /translation="MDNDWNGLADLIANLIAKYTGALDLDNLPDPTPAKNQEMKNSFD
                     MAKTQIETD"
     gene            591..2270
                     /locus_tag="VYN69_001927"
     CDS             591..2270
                     /locus_tag="VYN69_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000031864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEV1534138.1"
                     /translation="MSKEKIKVYLYTRVSTSIQIEGYSLEAQKSRMKAFAIYNDYEIV
                     GEYEDAGKSGKSIEGRIQFNRMMEDIKSGKDGVSFVLVFKLSRFARNAADVLSTLQIM
                     QDYGVNLICVEDGIDSSKDAGKLMISVLSAVAEIERENIRIQTMEGCIQKAREGKWNG
                     GFAPYGYKLEDGKLFINEEEAVAIRTIFDQYVNTTIGANGISKYLENHGIRKIPRQNG
                     KNPLFDAGLIRKILKNPVYNGKIAFGRRTLEKVHGTRNEYKQVEQDEYLISEGIHEAI
                     VSDEVWQAAQVKLKSQAKKYEHVNKGKDTRTHLLSGIVKCPICGVGMFGNKCIKKKKD
                     GTKYKDFYYYGCKHRQMIRGHKCTFSKQIREELLDDAVAEVIVKIVSNPKFASMMQEK
                     INMKVDTSEIEKEIDNYQKELRKSHSTKFKLIEEIDNLDVEDKHYKRRKQDLDDRLYR
                     MYDKIDELESSLIDAKAKKQTIEAEKLTGDNIYKVLIYFDKLYKVMNDVERRQLISAL
                     ISEIQVYEEKQSNGQWLKSITFKLPIIEENLNIDLDNDEQVECVSLLEKRS"
     gene            2538..3104
                     /locus_tag="VYN69_001928"
     CDS             2538..3104
                     /locus_tag="VYN69_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534139.1"
                     /translation="MVILELYQNDYSKDLVAFDSIEDGKAFVAQIPGYTLETEDGFEV
                     EYFNPKNIPDYMEIIFNGNIVPLSKFMFDPEENVNIIWKEISNLSLKNDRVIEGYSKI
                     DAYVVNNHEVKAYVETRETNYRKAKDFLESRGYEIDRSFFGSEDGEAILYRKKGIEVW
                     HFLCHLDPMFVEIEDVEGYVKEEVGEIQ"
BASE COUNT         1247 a          420 c          711 g          928 t
ORIGIN      
        1 tttgaacctt aacaattgaa tagaccaaga caagacgtta attatttttg gagcgtaata
       61 acctatccgt caagatcttt cggctgaata attcggcaaa aaaagttctg ctaagcaaaa
      121 agaaagtgag cgataagtaa gagtttgaat atagggaggg ataccctatc cgctatgaag
      181 aaaatgagga taatgatact tctaaggttg aagccggctc atccggaata gaggcggagg
      241 tgagattcct atgttgctaa tccaaagcac ttgtcaatgt caaaaaactt aattaaacaa
      301 ataattatta gatataaaac tttagaggag gtatatattg gacaatgact ggaatggatt
      361 ggctgattta atagcaaatc tgattgcaaa atatactggt gctttagatt tagataatct
      421 acccgatcca acgccagcaa aaaatcaaga gatgaaaaat agttttgaca tggcaaaaac
      481 acaaattgag acggactaat aaaagtgtta taatatactt gaaatgatag tccaaactta
      541 aattaaaaaa attatatatg atatagaaaa agtatattga ggttaatact atgtcaaaag
      601 aaaaaataaa agtatacctc tatacacgag tatctacgtc aatacagata gaggggtatt
      661 cgttagaggc acaaaaatca cgaatgaaag cttttgctat ttacaacgat tatgaaattg
      721 ttggagagta tgaagatgcc gggaagtctg gcaagtctat tgaaggtaga atacagttca
      781 atcgaatgat ggaagatata aaatctggaa aagatggagt atcttttgtt cttgtgttta
      841 agttatcaag atttgcaaga aatgccgctg atgttctttc tacacttcag ataatgcagg
      901 attatggagt taatcttatc tgcgttgaag atggcattga ttcatctaaa gatgctggga
      961 aactaatgat ttctgtttta tcagctgtgg ctgaaataga aagagaaaac atccgtattc
     1021 aaacaatgga aggttgcatt caaaaagcaa gggaaggaaa atggaatggt ggctttgctc
     1081 cgtatggata taaacttgaa gacggcaagc tgtttataaa tgaggaagag gcagttgcaa
     1141 taagaacgat tttcgaccag tatgtaaata ctacgatagg agccaatggg atatctaaat
     1201 acttagagaa tcatggaatt agaaaaatcc caagacagaa tggtaagaat cctttgtttg
     1261 atgcaggtct tataagaaag atattaaaga atcctgtata taatgggaaa atagcatttg
     1321 gaagaagaac tttagaaaaa gttcatggta caagaaatga atataagcag gttgaacaag
     1381 atgaatatct aatatctgaa gggatacatg aagctatagt ttccgatgaa gtttggcaag
     1441 ctgctcaggt taagctaaaa tctcaagcaa agaaatatga gcatgtgaat aaaggaaaag
     1501 atacacgcac acacttgctt tctggaattg taaaatgccc gatatgtgga gtgggaatgt
     1561 ttgggaacaa gtgtatcaag aaaaagaaag atggcacaaa gtataaagat ttttattact
     1621 atggttgtaa acataggcag atgataagag gtcataagtg tacattcagt aagcaaatta
     1681 gagaagaatt gttagatgat gctgttgcag aggtgattgt caagatagta agtaatccga
     1741 aatttgcttc tatgatgcaa gaaaaaatca acatgaaggt ggatacctct gaaatagaaa
     1801 aagaaataga taattaccaa aaggaattga gaaagagtca ttctacaaag tttaagctaa
     1861 ttgaggaaat agataattta gatgttgaag ataagcacta taagagaagg aaacaggatt
     1921 tagatgatag actatatcgt atgtatgaca aaatagatga attagaatca tcactaattg
     1981 atgcgaaagc aaagaaacag actattgaag ctgaaaaact tacaggagat aacatatata
     2041 aggttctgat ctattttgat aagctctata aagtcatgaa tgatgtagag cgtaggcagt
     2101 taatttcagc tttgatttct gaaattcaag tttatgaaga aaaacaatct aacggacaat
     2161 ggctaaaatc cattactttt aagctaccaa ttatagaaga aaatctcaac atagatttgg
     2221 acaatgatga acaagttgaa tgtgtaagtt tattagagaa acgtagttaa tcctcaaaag
     2281 gttccccaaa ctttttgagt cccactgacg catcacgctg aggcggtatc actgcttgta
     2341 cgagttgaag catcagcgaa gtaaaagtag atgttcagcc aatgcgacga ggtgagaccg
     2401 aaggtcgata aggtgtgttc tgggtatagg actcgtaaag taagtaggaa atatgatgag
     2461 atgatacaga gagcctgaat tggtcaggtg acgtgcttat ttacattagc ctgataatga
     2521 aaatggagag attatcgatg gttatattgg aattgtacca aaatgattat tctaaagatt
     2581 tagtagcatt tgattccata gaagatggga aagcctttgt ggctcaaatc ccgggataca
     2641 ctctggaaac agaagatggt tttgaagtgg agtattttaa tcctaaaaac ataccggatt
     2701 atatggaaat tatttttaac ggaaatattg ttcctttatc taaatttatg ttcgatcctg
     2761 aggaaaatgt aaacattatt tggaaagaga tttcaaattt atccctcaaa aatgatagag
     2821 tgatcgaagg atattcaaaa attgatgcat atgtcgttaa caaccatgaa gttaaagcct
     2881 atgtcgaaac aagagaaacg aactatcgta aagcaaaaga cttcttagaa agccgcggat
     2941 atgaaatcga cagaagcttt ttcggaagtg aagacggtga agcgattctt tacagaaaaa
     3001 aagggatcga agtttggcat ttcttgtgtc acttagaccc aatgtttgta gagattgaag
     3061 atgtagaagg atatgttaaa gaagaagttg gagagattca atagtcaacc aaagttcctt
     3121 gtgagtatat acctttgcta gtaaaagagt caaaacgtta taacgaaggg ggggaagaaa
     3181 acccttgagt gtaagtcttt ttcttgttta gagggaaagt attagagtag ccgctcataa
     3241 tttatactag atttgataac tgatttgaga cagattttat ttctataaat attgtgaaaa
     3301 tttttt
//
LOCUS       DAVLVP010000134         3280 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.134, whole genome shotgun sequence.
ACCESSION   DAVLVP010000134 DAVLVP010000000
VERSION     DAVLVP010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3280)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3280)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3280
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.134"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(189..800)
                     /gene="rpsD"
                     /locus_tag="VYN69_001929"
     CDS             complement(189..800)
                     /gene="rpsD"
                     /locus_tag="VYN69_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014407971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEV1534140.1"
                     /translation="MSRYTGPSWKQARRLGLSLTGTGKELARRNYVPGQHGPNNRSKL
                     SEYGLQLAEKQKLRFTYGVGEKQFRNLFVQATKIKGGILGFNFMLLLERRLDNVVYRL
                     GLATTRRQARQFVNHGHILVDGKRVDIPSYRVTPGQVISVREKSLKVPAILEAVEATL
                     GRPAFVSFDAEKLEGSLTRLPERDEINPEINEALVVEFYNKML"
     gene            complement(995..2047)
                     /locus_tag="VYN69_001930"
     CDS             complement(995..2047)
                     /locus_tag="VYN69_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEV1534141.1"
                     /translation="MKLKSYILVGYIISTLLTILVVFWAVQKMLIAKGEIYFLLGMTI
                     VASLVGAGISLFLLLPVFTSLGKLKEHAKRVAAKDFPSNLEVQGPVEFQQLGQTFNEM
                     SHDLQVSFDSLEESEREKGLMIAQLSHDIKTPITSIQATVEGILDGIIKESEQAHYLA
                     TIGRQTERLNKLVEELNFLTLNTARNQVETTSKDSIFLDKLLIECMSEFQFLIEQERR
                     DVHLQVIPESARIEGDYAKLSRILVNLVDNAFKYSAPGTKLEVVAKLEKDQLSISVTD
                     EGQGIAPEDLENIFKRLYRVETSRNMKTGGHGLGLAIARELAHQLGGEITVSSQYGLG
                     STFTLVLNLSGSENKA"
     gene            complement(2044..2742)
                     /locus_tag="VYN69_001931"
     CDS             complement(2044..2742)
                     /locus_tag="VYN69_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000520636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1534142.1"
                     /translation="MGKTILLVDDEVEITDIHQRYLIQAGYQVLVAHDGLEALELFKK
                     KPIDLIITDVMMPRMDGYDLISEVQYLSPEQPFLFITAKTSEQDKIYGLSLGADDFIA
                     KPFSPRELVLRVHNILRRLHRGGETELISLGNLKMNHSSHEVQIGEEMLDLTVKSFEL
                     LWILASNPERVFSKTDLYEKIWKEDYVDDTNTLNVHIHALRQELAKYSSDQTPTIKTV
                     WGLGYKIEKPRGQT"
     gene            complement(2909..>3280)
                     /locus_tag="VYN69_001932"
     CDS             complement(2909..>3280)
                     /locus_tag="VYN69_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEV1534143.1"
                     /translation="LDNIVATKKVTIKINVKETSDTANGSLSPSNSGSGVTPMNHNHA
                     TGTTDSMPADTMTSSTNTMAGENMAASANKMSDTMMSEDKAMLPNTGETQTSMASIGF
                     LGLALAGLLGGLGLKNKKEEN"
BASE COUNT          914 a          738 c          630 g          998 t
ORIGIN      
        1 ccataaaaga ttataccaca ttgtgtacta tttttggttc attttactat atcactttgg
       61 actatatcgg tctagttatg tctaaacgat tatagctgga tactaagaga aatcaaaaaa
      121 gcactaaggg agaacgtccc aaagtgctta atatcaaggc ctcaaagcct gtcttattca
      181 ataaaatatt acaacatctt gttgtagaat tcaacgacaa gtgcttcgtt gatttctggg
      241 ttgatttcgt cgcgttctgg caagcgagtc aatgaacctt ccaatttttc agcgtcgaat
      301 gatacgaatg ctggacgtcc aagagtagct tctactgctt caaggattgc tggaactttc
      361 aatgattttt cacgaactga gatcacttga cctggagtta cgcggtatga tgggatatca
      421 acgcgtttcc cgtcaacaag gatgtgaccg tggtttacga attgacgagc ttgacgacga
      481 gtagtcgcga gaccaagacg gtaaacaacg ttatccaaac gacgttccaa aagaagcata
      541 aagttgaaac ctaggattcc gcctttgatt tttgtagctt gtacgaacaa gttacggaat
      601 tgtttttcac ctacaccgta agtgaaacga agtttttgtt tttcagccaa ttgcaaaccg
      661 tattctgaca atttagaacg gttgtttggt ccgtgttgtc ctggtacgta gttacgacgt
      721 gccaattctt tacctgtacc tgtaagtgaa aggccaaggc gacgagcttg tttccaagat
      781 ggtcctgtat aacgtgacat atgaatgtcc tcctgatata aataatattt cggtggaaat
      841 agtcacttag aaagccctga ttcgtgcaga tgcccttcgc ctaaacagcc aaggttactt
      901 gtcataagac acctgttgac gagcttcatg ctttcctgct gatatttcac acaaaatcta
      961 ttctaccatg aatagctgga tttgtaaagg ggttttaggc tttattttca ctaccagaga
     1021 ggttgagaac gagggtaaag gtacttccta gaccgtactg gctgctgact gtgatttccc
     1081 cacccaattg atgggccaat tcacgcgcaa tcgcaagtcc taatccatga ccacctgtct
     1141 tcatgttacg cgaagtttcg acacgataaa ggcgtttgaa aatattttcc aaatcctctg
     1201 gggcaatacc ctgcccttca tcggtcacac tgattgaaag ctggtccttc tccagcttag
     1261 ccaccacttc cagcttggtt cctggagcag aatatttaaa agcgttatcg accagattca
     1321 ccaagatacg agaaagctta gcataatctc cctcaatccg ggcagactct gggattacct
     1381 gcaagtggac atctcttctc tcctgctcaa tcaaaaactg aaattcactc atgcactcaa
     1441 ttaagagctt gtccagaaaa atactgtctt tactggtagt ttccacctga tttctagctg
     1501 tgtttagggt caaaaaattc aactcctcaa ccagtttatt gagcctctcc gtctggcgtc
     1561 caatggttgc tagataatga gcttgctccg actccttgat aataccatcc aaaatccctt
     1621 ctaccgtcgc ttggatcgaa gtgataggag tcttaatatc atgcgacaac tgggcaatca
     1681 tcaagccctt ttctcgttcg ctttcttcca aggaatcaaa gcttacctgc aaatcatggg
     1741 acatctcatt aaaagtttgc cctaattgct gaaattctac aggaccttga acctccaaat
     1801 ttgaaggaaa atccttggcc gctacccgct tggcatgctc cttgagtttg cccaacgacg
     1861 taaagactgg caataggaga aagagactaa tcccagcacc gacaaggctg gcaacgatgg
     1921 tcatcccaag caaaaagtaa atctcgcctt tcgcaatcag cattttttga acagcccaaa
     1981 aaacaaccaa aatggttaag agggttgaaa taatatatcc aaccaaaata taacttttta
     2041 gtttcatgtt tgtcctctcg gtttctctat cttatatccc aacccccaaa ctgtcttaat
     2101 agtgggcgtt tggtcactac tatattttgc cagctcctgt cgaagagcat ggatatgcac
     2161 attcaaggta ttggtgtcat ccacgtagtc ttctttccag atcttttcat agaggtctgt
     2221 cttggagaaa actcgctctg gattgctagc taaaatccac agcaattcaa atgatttaac
     2281 agttaaatcc agcatttctt ctcctatttg aacttcatga ctactatgat tcatttttag
     2341 attgccaagg gaaatcagct ctgtttcgcc cccacgatga aggcggcgca aaatattgtg
     2401 gacacgcaaa accagctcac gtgggctaaa aggcttagca ataaaatcat ctgctcccaa
     2461 gctcaggccg taaatcttgt cctgttcact ggtcttagca gtaataaata ggaaaggctg
     2521 ctctggtgat aagtattgaa cctcactgat taaatcataa ccatccatcc gaggcatcat
     2581 gacatctgtg ataatcaaat caatcggttt tttcttgaac agctctagcg cttccagtcc
     2641 atcatgggct accaagacct gataacctgc ctgaattaag tatctctgat gaatatctgt
     2701 gatttctacc tcgtcgtcaa cgagtaaaat tgtctttccc atctgtctct cctttgataa
     2761 aaacagtgct atactgcttt aagtataaca ctatttttat tgttttacga tgattttaat
     2821 ctgctattga aagaaagtcc tgattgctga ttgagttgtt aaactagtct tagtcagccc
     2881 actatccatc atttatttcc ttagctgatt agttttcttc ttttttgttt ttcaaaccta
     2941 gaccaccgag taaacctgca agcgcaagcc caaggaaacc aatacttgcc attgatgttt
     3001 gagtctcacc agtatttggt agcatagctt tatcctctga catcatcgta tcagacatct
     3061 tgttagcaga agcagccatg ttttcacctg ccatcgtgtt ggtagaactt gtcatggtgt
     3121 cagcaggcat gctatctgta gtacctgtag catgattgtg attcatcgga gtcacgccag
     3181 aaccagagtt agaaggtgat aatgaaccat ttgctgtgtc tgatgtttct ttaacattta
     3241 tcttaatagt gactttttta gttgctacga tgttgtccaa
//
LOCUS       DAVLVP010000135         3139 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.135, whole genome shotgun sequence.
ACCESSION   DAVLVP010000135 DAVLVP010000000
VERSION     DAVLVP010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3139)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.135"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..165
                     /locus_tag="VYN69_001933"
     CDS             <1..165
                     /locus_tag="VYN69_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001818644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEV1534144.1"
                     /translation="VSFAISSKGEKMKVENISYRVDHRILFDNISFDTLSSGVTLITG
                     KNGTGKSTLL"
     gene            complement(531..1841)
                     /locus_tag="VYN69_001934"
     CDS             complement(531..1841)
                     /locus_tag="VYN69_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HEV1534145.1"
                     /translation="MIHLIMISAIALAIGIGYRTKINIGLLAIAFSYLIATTLMGLSP
                     KELLHFWPTSLFFTIFSVSLFYNVATTNGTLDVLAQHILYRTRTYPNALYMILYLMAT
                     LLSALGAGFFTTMAVCCPLAITLCQKADKHPLIGAQAVNWGASGGANLITSSSGIVFQ
                     GLFKQMGWEEQAFSLGNHIFIVSIIYPLIVLLLLSCYSHYSKGRTNSSLTIDQPPLLS
                     KVQRQTTLLMISSMVLVWLFPLLHLIFPNIAWIATYQKTFDIGFVSILMVCLALRLKL
                     GKQEAILAKVPWATIIMLCGMSLLMSLAVKSGLVTLIGHLMTTTIPHFWLPLFFCVIA
                     GVMSLFSSTLSVVAPALFPIIAIISAQNPQIDIHLLTTATVIGALSTNISPFSSAGSL
                     IQLSLPNIEERGLAFKKQIILGVPISLSLGLLTTWILILLASLS"
     gene            2078..2950
                     /locus_tag="VYN69_001935"
     CDS             2078..2950
                     /locus_tag="VYN69_001935"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000022746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HEV1534146.1"
                     /translation="MSIISTDLTPFQIDDTLKAALREDVHSEDYSTNAIFDHHGQAKV
                     SLFAKEAGVLAGLTVFQRVFTLFDAEVTFQNPHQFKDGDRLTSGDLVLEIIGSVRSLL
                     TCERVALNFLQHLSGIASMTAAYVEALGDDCIKVFDTRKTTPNLRLFEKYAVRVGGGY
                     NHRFNLSDAIMLKDNHIAAVGSVQKAIAQARAYAPFVKMVEVEVESLAAAEEAAAAGA
                     DIIMLDNMSLEQIEQAITLIAGRSRIECSGNIDMTTISRFRGLAIDYVSSGSLTHSAK
                     SLDFSMKGLTYLDV"
BASE COUNT          963 a          522 c          713 g          941 t
ORIGIN      
        1 gtttcttttg ctattagttc aaaaggagaa aaaatgaaag tagaaaatat ttcgtatagg
       61 gtggatcatc gtatattgtt tgataatatt tcttttgata ctttgagttc aggcgtgaca
      121 ttaattactg gtaaaaatgg tacaggaaag tcaactttac tatagtagat tgaaactaga
      181 atagtacaca tctacttata aaatattgtt agaaatcgat ttgactatcc tgatctattt
      241 gtcctggtct tatttcattt cactatattt caaattgagt atgacgaagt gcgctcccat
      301 gtcctgggaa cgcactttct tcatattttt catattcttg aatccatcga taaagactat
      361 tgggatgaat ttttaaagtt gaactaatca tttttacagg atgaggttta cagcagagag
      421 tttgaaggct ttatcaaagg tttttcttgg cataatgact tttcctcgtt tccacttaat
      481 tttgtgtcta ctttattata ccaagtccac ccttaagtta gataataaat ctaacttaag
      541 gaagctagaa ggatgagaat ccaggtggtc aagagtccca aacttaagct gatggggaca
      601 cccagaataa tttgcttttt gaaggcaagg ccacgttcct ctatattggg aagtgagagt
      661 tgaatgagag aaccagctga tgaaaagggt gagatattag tagatagagc gccaataacg
      721 gtggctgttg tgagtaagtg aatatcaatc tgaggatttt gagcactgat gatagcaatg
      781 atgggaaaga gggctggagc tacaacggat agggtggaac taaagagtga catcactccg
      841 gctatcacac aaaagaacag aggtaaccag aaatgaggaa tggttgttgt catgaggtgc
      901 cctatcagtg tgactaaacc tgacttgacc gctagagaca ttagtaagct catgccgcag
      961 agcatgataa ttgtagccca gggaacctta gctaaaatgg cttcttgctt ccctaatttg
     1021 agccttaagg cgaggcagac catgagtatt gagacaaagc caatatcaaa tgttttttga
     1081 taagtagcta tccaggcgat gtttgggaaa atgagatgca acaagggaaa aagccaaacc
     1141 aaaaccatgc tgctgatcat gagcaaggtg gtttgtcttt gaaccttgct gaggagtggt
     1201 ggttggtcaa tagtcaagga tgagtttgtt cttcccttac tatagtgact gtaacaggat
     1261 aataaaagca agacgatgag tgggtagata atgctgacga taaagatatg attgccaagt
     1321 gaaaaagctt gctcttccca tcccatttgc ttaaacaggc cttgaaagac aatgcctgag
     1381 ctactggtta tcaaattagc ccctcctgaa gctccccaat tgacagcttg agctccaatc
     1441 aaagggtgtt tgtccgcttt ttgacagagg gtaatcgcta gaggacagca aacggccata
     1501 gtagtgaaaa atccagcacc taaagcagac aaaagggttg ccatcaggta taaaatcatg
     1561 tagagggcgt tagggtaggt gcgtgtgcgg tagagaatgt gttgagccaa aacatcaaga
     1621 gtaccgttag ttgttgcaac gttataaaag agagagacgc taaaaatggt aaaaaagagt
     1681 gaggttggcc aaaaatgaag aagttctttg gggcttaatc ccatgagagt ggttgcgatg
     1741 aggtaagaaa aagcaatagc cagcaggcca atattgattt tggtgcggta accaattcca
     1801 atggctagag caatggcgct aatcattatt aaatgaatca ttgaattgtc ctttcagtta
     1861 gaatatggaa ataggttggc aattttggcg ctcaaaagac cacctgtttc ctattggtac
     1921 ttgactatta tacacaaaaa aaatacaatt gtcttgacaa ttgtattgac acttgttaga
     1981 attaaaaatg aggtattcag gcttgtgatt tcctatggaa gttaatagtg attgcctcta
     2041 gtgcttacaa gtgatattaa aaatagagga cttagtgatg tcaatcattt caactgattt
     2101 aacccctttt caaatagatg atacattgaa agcagccttg cgagaagatg ttcattccga
     2161 agattacagt accaatgcca tttttgatca tcatggccaa gccaaggtgt cgctttttgc
     2221 caaggaagct ggtgttttag cgggactaac cgtttttcaa agggttttta ccctatttga
     2281 tgccgaggtg accttccaga atcctcatca atttaaggat ggggatcgtt tgactagtgg
     2341 cgatttggtt ttagaaatca taggctcggt gagaagtctc ttaacatgtg aacgcgttgc
     2401 cttgaatttt ttacaacatt tatcagggat tgcttcgatg acagctgctt atgtagaagc
     2461 cttaggcgat gattgcatta aggtatttga tactcgaaaa actactccta atttacgtct
     2521 ttttgagaaa tatgccgtga gagttggcgg tggctataat catcgcttta atttatcaga
     2581 tgctatcatg ctaaaagaca atcacattgc ggcagtaggt agtgttcaaa aggcaattgc
     2641 tcaagcgcgt gcctatgccc cttttgtgaa aatggtcgag gtggaagtgg aaagccttgc
     2701 tgctgccgaa gaagctgcgg cggcgggtgc tgatattatc atgttggata atatgtcatt
     2761 ggaacagatt gaacaggcca ttaccctaat tgcaggacgt tctcggattg aatgttctgg
     2821 aaatattgat atgaccacta ttagccgttt tcgtggttta gcgattgatt acgtctccag
     2881 tggtagttta acccatagtg ctaagagtct tgatttttcc atgaagggtt taacctacct
     2941 tgatgtctaa gttgtaaaat aaactaactt tttaaaggat gtctttcctc tagaacgagt
     3001 tttatgtcag atagtttaaa cgcctcttca aatatagtaa aatgaaccaa aaatagtaca
     3061 caatgtggta taatcttctt atggcatatt caatagattt tcgtaaaaaa gttctctctt
     3121 attgtgagcg aacaggtag
//
LOCUS       DAVLVP010000136         2987 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.136, whole genome shotgun sequence.
ACCESSION   DAVLVP010000136 DAVLVP010000000
VERSION     DAVLVP010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2987
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.136"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            307..2808
                     /locus_tag="VYN69_001936"
     CDS             307..2808
                     /locus_tag="VYN69_001936"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014639013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEV1534147.1"
                     /translation="MSFYNHKEIEPKWQGYWAEHHTFKTGTDASKPKFYALDMFPYPS
                     GAGLHVGHPEGYTATDILSRYKRAQGYNVLHPMGWDAFGLPAEQYAMDTGNDPAEFTA
                     ENIANFKRQINALGFSYDWDREVNTTDPNYYKWTQWIFTKLYEKGLAYEAEVPVNWVE
                     ELGTAIANEEVLPDGTSERGGYPVVRKPMRQWMLKITAYAERLLNDLDELDWSESIKD
                     MQRNWIGKSTGANVTFKVKGTDKEFTVFTTRPDTLFGATFTVLAPEHELVDAITSSEQ
                     AEAVADYKHQASLKSDLARTDLAKEKTGVWTGAYAINPVNGKEMPIWIADYVLASYGT
                     GAVMAVPAHDQRDWEFAKQFDLPIVEVLEGGNVEETAYTEDGLHVNSDFLDGLNKEDA
                     IAKIVAWLEEKGCGQEKVTYRLRDWLFSRQRYWGEPIPIIHWEDGTSTAVPETELPLV
                     LPVTKDIRPSGTGESPLANLTDWLEVTREDGVKGRRETNTMPQWAGSSWYYLRYIDPH
                     NTEKLADEDLLKQWLPVDIYVGGAEHAVLHLLYARFWHKFLYDLGVVPTKEPFQKLFN
                     QGMILGTSYRDHRGALVATDKVEKRDGSFFHVETGEELEQAPAKMSKSLKNVVNPDDV
                     VEQYGADTLRVYEMFMGPLDASIAWSEEGLEGSRKFLDRVYRLITSKEILAENNGALD
                     KVYNETVKAVTEQIESLKFNTAIAQLMVFVNAANKEDKLYVDYAKGFIQLIAPFAPHL
                     AEELWQTVAETGESISYVAWPTWDESKLVEDEIEIVVQIKGKVRAKLMVAKDLSREEL
                     QEIALADEKVKAEIDGKEIVKVIAVPNKLVNIVVK"
BASE COUNT          916 a          603 c          670 g          798 t
ORIGIN      
        1 agattcagtc tactatacgt tcttcataaa tcaaaaagcg acataggtag tcggctattt
       61 attgtgaata cattaattag cattccagtt ttatcttcgg tctaaaataa gtattttgtg
      121 ctatacgaga taagcttctt gacttactcc ttgatttact gcataacaat gggataaaaa
      181 gtgggagata gagcaattca tagtcatcaa aattaatgag atacagtata cagtttttcc
      241 tttaaacaca tttcaaattc cctcaaaaat ggtataatag taacatcaca aaattggaga
      301 gagaccatga gtttttacaa tcataaagaa attgagccta agtggcaggg ctactgggca
      361 gaacatcata catttaagac aggaacagat gcatcaaaac ctaagtttta tgcgcttgat
      421 atgttccctt atccgtctgg agctggtctg cacgtaggac acccagaagg ttatactgca
      481 accgatatcc tcagtcgtta caaacgtgcg caaggctaca atgtccttca cccaatgggt
      541 tgggatgctt ttggtttgcc tgcagagcaa tacgctatgg atactggtaa tgacccagca
      601 gaatttacag cggaaaacat tgccaacttc aaacgtcaaa tcaatgcgct tggattttct
      661 tatgactggg atcgtgaagt caatacaaca gatccaaact actacaagtg gactcaatgg
      721 attttcacca agctttacga aaaaggcttg gcctatgaag ctgaagtgcc agtaaactgg
      781 gttgaggaat tgggaactgc cattgccaat gaagaagtgc ttcctgacgg aacttctgag
      841 cgtggaggct atccagttgt ccgcaaacca atgcgccaat ggatgctcaa aatcacggct
      901 tacgcagagc gcttgctcaa tgacttagat gaactagatt ggtcagagtc tatcaaggat
      961 atgcaacgca actggattgg taaatcaact ggtgccaatg taactttcaa agtaaaagga
     1021 acagacaagg aatttacagt ctttactact cgtccggaca cacttttcgg tgcgactttc
     1081 actgtcttgg ctcctgaaca tgaattagta gacgctatca caagttcaga gcaagcagaa
     1141 gctgtagcag actataaaca ccaagccagc cttaagtctg acttggctcg tacagacctt
     1201 gctaaagaaa aaacaggggt ttggactggt gcttatgcca tcaaccctgt caatggtaag
     1261 gaaatgccaa tctggattgc agactatgtc cttgctagtt atggaacagg tgcggttatg
     1321 gctgtgcctg cccacgacca acgtgactgg gaatttgcca aacaatttga ccttccaatc
     1381 gtcgaagtac ttgaaggtgg aaatgtcgaa gaaactgcct acacagagga tggcctgcat
     1441 gtcaattcag acttcctaga tggactcaac aaagaagacg ctattgccaa gattgtggct
     1501 tggttggaag aaaaaggctg tggtcaggag aaggttacct accgtctccg cgactggctc
     1561 tttagccgtc aacgttactg gggtgagcca attccaatca ttcattggga agatggaact
     1621 tcaacagctg ttcctgaaac tgaattgccg cttgtcttgc ctgtaaccaa ggatatccgt
     1681 ccttcaggta ctggtgaaag tccactagct aacttgacag attggcttga agtgactcgt
     1741 gaagatggtg tcaaaggtcg tcgtgaaacc aacactatgc cacaatgggc tggttcaagc
     1801 tggtactacc tccgctatat tgatccacac aatactgaga agttggctga tgaggacctc
     1861 ctcaaacaat ggttgccagt agatatctac gtgggtggtg cagagcatgc tgttcttcac
     1921 ttgctttacg ctcgtttctg gcacaaattc ctctatgacc tcggtgttgt tccgactaag
     1981 gaaccattcc aaaaactctt taaccaaggg atgattttgg gaacaagcta ccgtgaccac
     2041 cgtggtgctc ttgtggcaac cgacaaggtt gaaaaacgtg atggttcctt cttccatgta
     2101 gaaacagggg aagagttgga gcaagcgcca gccaagatgt ctaaatcgct caagaacgtt
     2161 gttaacccag acgatgtggt ggaacaatac ggtgccgata cccttcgtgt ttatgaaatg
     2221 tttatgggac cactcgatgc ttcgattgct tggtcagaag aaggattgga aggaagccgt
     2281 aagttccttg accgagttta ccgtttgatt acaagtaaag aaatccttgc ggaaaacaat
     2341 ggtgctcttg acaaggttta caacgaaaca gtcaaagctg ttactgagca aattgagtct
     2401 ctcaaattca acacagctat tgcccaactt atggtctttg tcaatgctgc taacaaggaa
     2461 gataagcttt atgttgacta tgccaaaggc tttattcaat tgattgcacc atttgcacct
     2521 cacttggcag aagaactctg gcaaacagtc gcagaaacag gtgagtcaat ctcttatgta
     2581 gcttggccaa cttgggacga aagcaaattg gttgaagatg aaattgaaat tgtcgtccaa
     2641 atcaaaggaa aagttcgtgc caaactcatg gttgctaaag atctatcacg tgaagaatta
     2701 caagaaatcg ctttagctga tgaaaaagtc aaagcagaaa ttgacggtaa ggaaatcgtg
     2761 aaagtaattg cggtaccgaa taaactcgtt aatatcgtcg ttaaataacg agtttattag
     2821 ctctatctgc caccttcaat agtccactgg actattgaag ccaactaaat tagttaacat
     2881 tgttgtgaaa taaaaagaag taactctaga gtatgaactt tagagttact tttagaatag
     2941 gtcagaatga atagattagt gaacgatagc aggaagtgtg aaatttt
//
LOCUS       DAVLVP010000137         2964 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.137, whole genome shotgun sequence.
ACCESSION   DAVLVP010000137 DAVLVP010000000
VERSION     DAVLVP010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.137"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(130..663)
                     /locus_tag="VYN69_001937"
     CDS             complement(130..663)
                     /locus_tag="VYN69_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000775318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEV1534148.1"
                     /translation="MKLPKEGDFITIQSYKHDGSLHRTWRDTMVLKTTENAIIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNIIAMIRDNGTSYYCNMASPYYLDEEALKYI
                     DYDLDVKIFTDGEKRLLDVEEYERHKRKMNYSDDLDYILKEHVKILVDWINNGRGPFS
                     EAYVNIWYKRYVELKNR"
     gene            complement(752..1528)
                     /gene="recX"
                     /locus_tag="VYN69_001938"
     CDS             complement(752..1528)
                     /gene="recX"
                     /locus_tag="VYN69_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000705102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEV1534149.1"
                     /translation="MKITKLEKKKRLYLMELDNDDKCYITEDTIVRFMLSRDKVISEE
                     ELKEIQDFAQFSYGKNLALYHLSFKARTEKEVREYLKKYDIDKNIVSQVIANLKEDKW
                     INDGQYAYAIINANQLSGDKGPYVLTQKLAQKGISKSTIEEILNDFDFSEVAQRVANK
                     LLKKYEGKLPARALQDKIIQNLTNKGFSYFDAKIAFDELDSQVDEETTQELIFKELDK
                     QYTKYARKYEGYELKQRLTQVLARKGYDFSDIASALREYL"
     gene            1566..2945
                     /gene="rlmD"
                     /locus_tag="VYN69_001939"
     CDS             1566..2945
                     /gene="rlmD"
                     /locus_tag="VYN69_001939"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEV1534150.1"
                     /translation="MNLKVKQKIPLKIKRMGINGEGIGFYQKTLVFVPGALKGEDIYC
                     QITSIRRNFVEAKLLKVNKKSKFRIVPSCTIYNECGGCQIMHLHYDKQLEFKTDLLHQ
                     ALKKFAPAGYENYEIRPTIGMQEPKYYRAKLQFQTRKFKNQVKAGLYAQNSHYLVELK
                     DCLVQDKETQVIANRLAELLTYHQIPITDERKVLGVRTIMVRRARKTGQVQIIIVTNR
                     QLNLTQLVKELVKDFPEVVTVAVNTNTAKTSEIYGEKTEIIWGQESIQEGVLNYEFSL
                     SPRAFYQLNPEQTEVLYSEAVKALDVDKEDHLIDAYCGVGTIGFAFAKKVKTLRGMDI
                     IPEAIEDAKRNAKRMGFDNTHYEAGTAEEIIPRWYKEGYRADALIVDPPRTGLDDKLL
                     DTILTYVPEKMVYISCNVSTLARDLVRLVEVYDLHYIQSVDMFPHTARTEAVVKLIKK
                     IRKSSVNRK"
BASE COUNT          940 a          539 c          539 g          946 t
ORIGIN      
        1 gcataaatct ctttctagta atgtgttgta actctgctat aatggattta ttcctttttg
       61 tgtttacaca atttatttta tactaccaaa aaaacaaggt caggattttg ttcctgacct
      121 ttgacaactt taccgattct ttagttctac atagcgcttg taccaaatgt ttacataggc
      181 ttctgagaaa ggaccacgtc cattgttaat ccaatcaaca agaattttga catgttcttt
      241 taaaatatag tccaagtcat cagaataatt cattttgcgt ttgtgacgct cgtactcttc
      301 aacgtccaag agacgttttt ccccatctgt aaaaatttta acatccaaat cgtaatcaat
      361 atacttcagt gcttcttcat ccagatagta ggggctagcc atattgcaat agtaagaagt
      421 tccattatca cgaatcatgg caatgatatt aaaccaatat ttcttgtgaa agtaaacaat
      481 agccggttct cgagtgaccc aacgacgacc atcactttcg gtaacaagtg tatgatcgtt
      541 gacaccaata atggcgtttt ctgttgtttt tagtaccatg gtgtcccgcc aagttcggtg
      601 gagactccca tcatgcttat aactttgaat tgtaataaag tcgccttctt ttggaagctt
      661 cataactaac caactttcta caatttataa gtttatcatt tactattgta ccataaaatt
      721 acctaaaatc tgtgaatttc acttggaaat attaaagata ttctctaaga gcgcttgcta
      781 tatccgaaaa atcgtagccc tttcgtgcta aaacttgagt taaacgctgc ttcagttcgt
      841 atccttcata ctttcgggca tacttagtat attgcttatc aagttccttg aagatgagtt
      901 cctgagtcgt ttcttcatca acttgactat ccaattcgtc aaaggcaatt ttagcatcaa
      961 aataagagaa gcccttgtta gtcaagttct ggataatctt atcttgcagg gcacgagctg
     1021 gaagttttcc ctcatatttt ttcaatagtt tattggctac acgttgagca acttccgaaa
     1081 aatcaaaatc attcaagatt tcttctatag tagattttga aattcctttt tgtgctaatt
     1141 tctgagtcag tacataaggt cccttgtctc ctgaaagttg attggcattg atgatagcat
     1201 aagcgtactg accatcatta atccacttat cttctttaag attagcaata acttgagaaa
     1261 cgatgttttt atcaatatcg tattttttca gatattctct gacttctttt tcagtgcgtg
     1321 ctttaaagga taagtggtag agggccagat tcttaccata agaaaattga gcaaagtctt
     1381 gaatctcttt caattcctct tcgcttatca ccttatctct cgataacata aaacgaacaa
     1441 ttgtatcttc ggtgatatag catttgtcgt cattatcaag ctccatcaga tagagtcttt
     1501 ttttcttttc aagttttgtg attttcatag ctctattata actcaaaatg tgataagata
     1561 ggggtatgaa tctgaaagtg aaacaaaaaa taccattaaa aatcaagcgc atgggaatta
     1621 acggtgaggg aatcggcttt taccaaaaaa cattagtctt tgtaccagga gctctcaaag
     1681 gcgaagatat ctattgtcag attacttcta ttagacgcaa ctttgttgaa gcaaaattac
     1741 tgaaggtcaa caagaagtct aaatttcgaa ttgtgccatc ttgtactatt tataatgaat
     1801 gcggaggctg ccaaatcatg cacctgcatt atgataagca gctggagttc aagacggact
     1861 tacttcatca agcgctgaaa aaatttgctc ctgcaggata tgaaaattat gaaattcgtc
     1921 caactattgg aatgcaggaa ccaaaatatt acagagctaa gttacaattt cagactcgaa
     1981 aatttaaaaa tcaggtcaag gcgggcttat atgcacaaaa ctctcactat ttagtagagt
     2041 tgaaagactg cctggtacaa gataaggaaa cccaagtgat tgctaatcgc ttagcagaat
     2101 tacttactta tcaccagatt ccaatcacgg atgagagaaa agttctaggt gtccgtacta
     2161 ttatggtccg acgcgcgaga aagaccggac aggttcagat tattattgtt acaaaccgcc
     2221 agcttaattt aactcaattg gtaaaagagt tggttaaaga tttcccagaa gttgtgacag
     2281 tagctgttaa tacaaataca gctaaaacca gtgagatata tggtgaaaag acagagatta
     2341 tctgggggca agagagtatt caagaaggtg tactcaatta tgaattttca ctatcccctc
     2401 gagcttttta tcaactaaat cctgagcaaa cagaagtcct ctatagcgaa gcagtaaaag
     2461 cgctggatgt tgataaagaa gaccatttga ttgacgctta ttgtggagtt ggaacgattg
     2521 gatttgcctt tgcaaagaaa gtaaaaacac tcagaggtat ggatattatt ccagaagcta
     2581 ttgaagatgc caagcgaaat gctaaaagaa tgggatttga caatactcat tatgaagctg
     2641 gaacggcaga agagattatt cctcgttggt acaaggaagg ctaccgagca gatgctttga
     2701 ttgttgaccc accacgtaca ggtctggatg ataagttatt agatactatt cttacttatg
     2761 taccagaaaa aatggtttat atttcttgta atgtttcgac cttggctcgt gatttggtac
     2821 gcttagtaga agtctatgat cttcattata tccagtcggt cgatatgttc ccacatacag
     2881 ctcgaactga agctgttgta aaattaataa aaaaaattag aaaaagtagt gtcaatcgaa
     2941 agtgattatg tcccccaaaa atgg
//
LOCUS       DAVLVP010000138         2785 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.138, whole genome shotgun sequence.
ACCESSION   DAVLVP010000138 DAVLVP010000000
VERSION     DAVLVP010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2785)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2785)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2785
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.138"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..885
                     /locus_tag="VYN69_001940"
     CDS             <1..885
                     /locus_tag="VYN69_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEV1534151.1"
                     /translation="LAAMKIKDSLDASKEGLMEIFYLSPKIERLKEIQNQDLQEGDDY
                     SLKKFDIDLKDVEFAYNKDAKVLNGVSFKAKQGEVTALVGASGCGKTTILKLISRLYD
                     YDKGQILIDGKDIKEISTESLFDKVSIVFQDVVLFNQSVMENIRIGKQDASDEEVKRA
                     AKLANCTDFIEKMDKGFDTVIGENGAELSGGERQRLSIARAFLKDAPILILDEITASL
                     DVNNEKKIQESLNNLVKDKTVVIISHRMKSIENADKIVVLQNGRVESEGKHEELLQKS
                     KIYKNLIEKTKMAEEFIY"
     gene            896..1480
                     /locus_tag="VYN69_001941"
     CDS             896..1480
                     /locus_tag="VYN69_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEV1534152.1"
                     /translation="MDNKKLKVKDLVSIGVFGVIYFAFMFGVGMMGLIPILFLIYPTV
                     LAIVAGTVVMLFMAKVQKPWALFIFGMISPLVMFAAGHTYVVVVLSLIVMIIAELIRK
                     IGNYNSFKYNMLSYAIFSTWICSSLMQMLLAKEKYMEWSLMTMGKDYVDVLEKLITYP
                     HMALVALGAFLGGILGAYIGKALLKKHFEKAGIV"
     gene            1480..2175
                     /locus_tag="VYN69_001942"
     CDS             1480..2175
                     /locus_tag="VYN69_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001141947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEV1534153.1"
                     /translation="MPTFSSNAPFNPNPISKILVVFITGLTVMHSINIRFELAIVCLI
                     GILLYLNGYKKTLFKWIILCGILYSLPNFMVLSELNPIVKMFLSIPIVIRMFILPFMA
                     ASFMIKTSDVGAIISSMDKLKISKNVSIPIAVMFRFFPSFKEEKKNIKMAMRVRGINF
                     KNPVKYLEYVSVPLLIISSNISDDIAKAAETKAIENPIAKARYIRVKIQLIDFVYVLA
                     VAGLIVGGLIWLK"
     gene            2163..>2785
                     /locus_tag="VYN69_001943"
     CDS             2163..>2785
                     /locus_tag="VYN69_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002837029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1534154.1"
                     /translation="MVEIKNLSLDYGEEHILDDISLSIAEGECVLFTGKSGNGKSSLI
                     NSINGLAVRYDNAKTKGEIIIDGKNIKNLELYQISMLVSTVFQNPKTYFFNVNTTLEL
                     LFYLENIGLAREEMDRRLKDMLEIFPIKNLLNRNIFNLSGGEKQILCIAASYIAGTKI
                     IVMDEPSSNLDIKSISVLAKMLKILKEKGISIIVAEHRIYYLMDIVDR"
BASE COUNT          994 a          363 c          542 g          886 t
ORIGIN      
        1 ctagctgcta tgaagataaa agactcttta gatgcatcta aagagggctt gatggaaata
       61 ttttatttat cgcccaaaat agaaagatta aaagaaattc aaaatcaaga tttacaagaa
      121 ggcgatgact atagcttaaa aaaatttgat attgatctaa aagatgttga gtttgcctac
      181 aataaagacg caaaagtttt aaatggtgta agttttaaag ctaagcaggg agaggtcact
      241 gctttggtag gtgcaagtgg ctgcggtaaa acaactatct tgaaacttat atcaagactt
      301 tatgattatg acaagggaca aatcttaatt gatggcaaag atataaagga aatatcaaca
      361 gaatcccttt ttgataaggt gtctattgtt ttccaagatg tggttctctt taatcaaagc
      421 gttatggaaa atattagaat cggtaagcaa gatgcaagtg acgaagaggt taaaagagca
      481 gcaaaacttg caaattgcac agattttata gaaaaaatgg ataaaggttt cgatacagtt
      541 attggtgaga acggagctga gctatcagga ggagaaagac aaagattatc aatagccaga
      601 gccttcttaa aagatgcgcc gatattgatc ttagatgaga taacagcaag ccttgatgtt
      661 aacaacgaga aaaagattca agagtcttta aataatttag ttaaagataa aactgttgta
      721 atcatttcac atagaatgaa atccatagaa aatgcagaca agatagtagt tcttcaaaac
      781 ggaagagtag aaagcgaagg taagcatgaa gagcttttac aaaaatcaaa aatttacaaa
      841 aatttaatag aaaagacaaa aatggcagaa gaatttattt attaggagga ctacaatgga
      901 taataaaaaa ttaaaagtaa aagatttagt aagcatcggt gtttttggcg taatttattt
      961 tgccttcatg tttggagttg gtatgatggg cttgattcca atattgttct taatataccc
     1021 gacagtatta gccatagttg caggaactgt tgttatgtta tttatggcta aggttcaaaa
     1081 gccatgggca ctatttatat ttggtatgat atcaccactt gtgatgtttg cagctggtca
     1141 tacctacgta gttgtggttt tatcacttat agtaatgata atagcagaat taattagaaa
     1201 gattggtaat tataattcat ttaaatacaa tatgctttct tatgcaatct tcagcacatg
     1261 gatatgtagc tctttaatgc aaatgctttt agcaaaagaa aaatatatgg agtggtcttt
     1321 gatgactatg ggaaaagatt atgttgatgt attagaaaag ttaataactt atcctcacat
     1381 ggctttagta gccttaggtg ctttcttagg aggaattctt ggagcatata taggcaaggc
     1441 tctattgaaa aaacactttg aaaaagcagg catcgtataa tgcctacttt ttcttcaaat
     1501 gctcccttta atcccaatcc gatctctaag atattagtcg tatttatcac aggcttaact
     1561 gttatgcata gcataaacat ccgttttgag ctggcaattg tttgtcttat tggtattttg
     1621 ctttatttga atggatacaa aaaaacactt ttcaaatgga ttatattgtg tgggatactt
     1681 tactccttgc ctaattttat ggtgttatct gaattaaacc ctatagttaa gatgtttttg
     1741 agtataccta ttgttattag aatgtttatt ttaccattta tggcagcaag ctttatgata
     1801 aagacctcgg atgtaggcgc aataatttca tcgatggata agcttaagat ttcaaagaat
     1861 gtatccatac ctattgcggt tatgtttaga ttcttcccat cttttaagga ggagaagaaa
     1921 aacatcaaaa tggctatgag agtaagaggg ataaatttta aaaacccagt caaatatctt
     1981 gaatatgttt ctgtgccact actcattata tcatctaata tatcagatga cattgcaaaa
     2041 gcggcagaaa caaaggcaat agaaaatcca attgccaagg ccagatacat tcgcgtaaag
     2101 atacagctaa ttgattttgt ttatgtttta gcggttgctg gacttattgt gggaggctta
     2161 atatggttga aataaaaaat ttaagtcttg attatggtga agagcatata ttagatgata
     2221 tatcactatc catagccgag ggagagtgcg tgctatttac aggaaaaagt ggaaatggta
     2281 agtcatcttt aataaattca atcaatggac tagctgtaag gtatgataac gcaaagacaa
     2341 agggcgaaat aattattgat ggtaagaata taaaaaattt ggaactttat caaatctcaa
     2401 tgcttgtttc aactgttttt caaaatccta agacatattt ttttaatgtc aatacgacat
     2461 tagaattatt attttatttg gaaaatatcg gtcttgcaag agaagagatg gacaggcgtt
     2521 tgaaggatat gcttgagata ttcccgataa aaaatctttt gaacagaaat atatttaatc
     2581 tatccggcgg tgaaaaacaa attctttgca ttgcagcttc ttatatagca ggtacaaaga
     2641 ttatagttat ggatgagcct tcatcgaatt tagatattaa aagcataagt gttttggcaa
     2701 agatgctaaa gatattaaaa gagaaaggca taagcataat tgttgcagag catagaattt
     2761 attatttgat ggacatagtt gaccg
//
LOCUS       DAVLVP010000139         2696 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.139, whole genome shotgun sequence.
ACCESSION   DAVLVP010000139 DAVLVP010000000
VERSION     DAVLVP010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.139"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            13..1014
                     /locus_tag="VYN69_001944"
     CDS             13..1014
                     /locus_tag="VYN69_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000789954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEV1534155.1"
                     /translation="MKNINGKKVTIYDIARLSGFSPKTVSRVINGGVNVKEETYQAIQ
                     KVIEELSYIPNAYAKNLTKKEAINILISVKKIDSFPLIWFHTLLDKVLRTCKEFGVNA
                     IVEYFGEEDTISNSIISSTGSLVDGVIVFYESVDDIRIQYLKKNHMPFLVFGESQTSG
                     VVYVSNNNFQATYDMMKAVTEEKFKNMWLLMGGESHVNKDRERGVRSFLNDKNYFMDL
                     KVIYGLSTIDSVYSYAMQHLTTQNYPDIIFVSGDEKVQGLIRACYEKGILIPDDISII
                     GFDNIPISQYYTPALSTIAPNYVKLAKEMIEGVLAIIKGESVTSVEVSPKFVRRQSF"
     gene            complement(1458..>2696)
                     /locus_tag="VYN69_001945"
     CDS             complement(1458..>2696)
                     /locus_tag="VYN69_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY-like element IS1202 family transposase"
                     /protein_id="HEV1534156.1"
                     /translation="RNRKPKHAIPDEIKERILKKYLSYETYKPNVLHFCELLAEEEGI
                     KLSDTTVRKILYKKNILSPKSHRKTKKRVRKQAKLNLNQPLDNPILPTAKDFLEDPKK
                     VHPSRPRKKFAGELIQMDASPHAWFGPETTNLHLAIDDASGNILGAYFDKQETLNAYY
                     HVLEQILANHGIPLQMKTDKRTVFTYQASNSKKMEDDTYTQFGYACHQLGILLETTSI
                     PQAKGRVERLNQTLQSRLPIELERNKIHTLEEANTFLLSYIQTFNEQFGNKTKLSVFE
                     EAPNPSERNLILARLAERVVDSGHHIRFQNRCYIPTEQGKEVYFIRKTKALVLKAFDG
                     DIYLNIADKIYHTKELLDHELYSKNFEQEPEQKKERRKYIPPQTHPWKLTSFKQYLHK
                     NKKDYEEFTSEEIHSPQLQV"
BASE COUNT          766 a          404 c          572 g          954 t
ORIGIN      
        1 tagaggactg aaatgaaaaa tataaatggg aaaaaggtaa ctatttatga tattgcacgt
       61 ttatcaggtt tttctccaaa aacagtttca cgagttatta atggtggagt aaatgttaaa
      121 gaagaaactt atcaagcgat tcaaaaagtt atagaggaat tgtcgtatat tccaaatgcg
      181 tatgcaaaaa atttaactaa aaaagaagct ataaatatat tgatttctgt aaaaaaaatc
      241 gactcttttc ctttaatttg gtttcatacg ctgttagata aagtgttacg gacgtgtaaa
      301 gaatttggtg ttaatgctat agttgagtac tttggagagg aggatacaat tagtaattca
      361 attatatcaa gcacaggtag cctagtagat ggtgttattg ttttttatga aagtgtagat
      421 gatatcagaa ttcagtattt aaagaagaac catatgcctt ttcttgtttt cggtgaatct
      481 caaacgtctg gagtagtcta tgtatctaat aataattttc aagctactta tgatatgatg
      541 aaagctgtaa ctgaagaaaa atttaaaaat atgtggttgc ttatgggagg agaatctcat
      601 gttaataagg atcgtgaaag aggtgtgcgt tcttttctga atgataaaaa ttactttatg
      661 gatttaaaag tcatttatgg cttatctaca attgattcag tttattctta tgcaatgcag
      721 cacttgacta cccaaaatta tccggacatt atttttgttt caggagatga aaaggttcag
      781 ggcttgattc gtgcgtgcta tgagaaagga attcttattc cagatgatat ttctattatt
      841 ggatttgata atattcctat ttctcagtat tatactccag ctttgtctac aattgctccc
      901 aattatgtta aattggctaa agaaatgata gaaggtgttt tagcaattat taaaggggaa
      961 agtgtcacat ctgttgaagt ttctcctaaa tttgttagga gacagagttt ctagtattgt
     1021 ttaattcttg aaaatcctag tggaaaagat ggagtgttct aaaataagtt ctgtttagcc
     1081 tccattcagt ttctattgag ttggatccaa tttctaacaa gtgggacatg ttcatgttcg
     1141 atctaaggaa gacatggtca cattcaaatg atattttcaa aaaaatcgta aaaaagtctg
     1201 tcaatcgaaa gtgattatgt ccccaaaaaa tggagagttt gagggcaatt attttgattt
     1261 catttgacac gaggaaccct tgaagctgtt ttgttagcta aaccaaggct agtcttagcc
     1321 ttggctcgcc agcctaacag ctccaagcct cttatcaaat gaaatacttg tcttacactc
     1381 ctcactttcc ctctcctaaa tgtaagggat agagtgactt cctttaagga aaagcttccc
     1441 atttagcgaa aaatagatta tacttgtagt tgaggagaat gaatctcctc actagtaaac
     1501 tcttcataat cctttttgtt tttatgaagg tattgtttga aagatgtgag tttccacgga
     1561 tgggtttgtg gagggatata cttgcgtctt tctttttttt gttctggttc ttgttcaaag
     1621 tttttcgaat agagttcatg atctagtagc tcctttgtgt gatagatttt gtcagcgata
     1681 ttgaggtaga tgtcaccatc aaatgctttt agaactaatg ctttcgtctt tctgatgaaa
     1741 tagacttctt ttccttgttc ggtaggaata tagcaacggt tttggaatcg gatatggtgt
     1801 ccactatcga cgactctctc cgccagtcta gctagaatga gattccgttc agaggggtta
     1861 ggagcctcct caaaaacaga gagttttgtc ttatttccaa actgttcatt aaaggtttgg
     1921 atgtaggaaa gaaggaaagt attggcttct tccaatgtat gaatcttgtt tcgttccagt
     1981 tcgataggca ggcgtgattg tagtgtctga ttgagtcttt cgaccctccc tttagcttga
     2041 gggatagagg tggtctcaag gagaatccct agttggtgac aggcgtatcc aaattgtgta
     2101 taggtgtcgt cctccatttt cttagagttg gatgcttgat aggtaaagac cgttctctta
     2161 tcagttttca tttgaagggg aatgccgtga ttggctaaga tttgttcgag gacatggtag
     2221 taggcattca aggtctcttg tttgtcaaaa taagccccta ggatattgcc ggaagcatca
     2281 tcaatggcta agtgtaagtt ggtggtttct ggtccaaacc aggcatgagg gctggcatcc
     2341 atttgaatga gttctccagc aaatttcttt ctgggtctac taggatgtac ctttttaggg
     2401 tcttccagga agtctttagc cgtcggtaag attggattgt ctaggggttg attcaggttc
     2461 agtttagctt gttttcttac tctcttcttt gtctttctgt gagacttagg agaaaggatg
     2521 tttttcttat agagtatttt tctaacagtt gtatcagaga gcttaattcc ctcttcttca
     2581 gctagtaatt cacagaaatg aaggacattt ggtttatatg tttcatagga gaggtatttc
     2641 tttaggatac gttctttgat ttcatcaggg attgcatgtt ttggttttcg atttct
//
LOCUS       DAVLVP010000140         2650 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.140, whole genome shotgun sequence.
ACCESSION   DAVLVP010000140 DAVLVP010000000
VERSION     DAVLVP010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.140"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(282..605)
                     /gene="tnpB"
                     /locus_tag="VYN69_001946"
                     /pseudo
     CDS             complement(282..605)
                     /gene="tnpB"
                     /locus_tag="VYN69_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            complement(865..1164)
                     /locus_tag="VYN69_001947"
     CDS             complement(865..1164)
                     /locus_tag="VYN69_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000767202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEV1534157.1"
                     /translation="MKLINTTNSHSQLVKSQLESTDATLVEVYSAGNTDVIFTQAPLH
                     YEILISNKHRAIREPEIETIQEFFLKRKIDKTSVDEANIKTLYSEKLIGISIPIK"
     gene            complement(1329..1784)
                     /locus_tag="VYN69_001948"
     CDS             complement(1329..1784)
                     /locus_tag="VYN69_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001811998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEV1534158.1"
                     /translation="MQWQTQLSEKKANTDYIARYGVYAVIPNPEQKQIVLVQEPNGAW
                     FLPCGEIEAGENHQEALKRELIEELGFTAEIGTYYGQADEYFYSRHRDTYYSNPAYLY
                     EATPFKEVQKPLENFNHIAWFPIDEAIKNLKRGSHKWAIESWKKQHKID"
     gene            1861..2650
                     /locus_tag="VYN69_001949"
                     /pseudo
     CDS             1861..2650
                     /locus_tag="VYN69_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611222.1"
                     /note="programmed frameshift; internal stop; incomplete;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
BASE COUNT          844 a          473 c          492 g          841 t
ORIGIN      
        1 ctcgtttgga agatattcta gaagatagga tagatatttc tcgctattta tactagacta
       61 aaatcaaaaa gcattataca atagtaatat gaaatcaatt aaagaagaaa tccaaaccat
      121 caaaacactt ttaaaagact ctcgtacagc taaatatcat aaacgccttc aaatcgttct
      181 atagtaaaac gaaacaagaa caggacaaat cgatcaggac agtcaaatcg atttctaaca
      241 atattttaga agcagaggtg tactattcta gtttcaatct actatatttt tggagtgata
      301 gaaaagccct tcatcagcca atctacttgt tcaggtgcga gagctttgac atccttttct
      361 gtacttagcc aagtcagttt tccgttctca aagcgtttat atagtagcca aaatccttga
      421 ccatcccagt aaagggcttt aaagcggtct ttacgtccac cacaaaagag aaagacttga
      481 ccggagaaag aatccaattc aaagtgggtt ttaactacat aggctaatga gtctattccc
      541 tacctcatat ctgtcttacc acaaacaagg tgaacttgac ctaaatcact tagttgaatt
      601 atcatagtac aatacctttc ctccgataat tattttttat ctagtatact ggaagttggg
      661 gaattaggat agataccttg ttatgacgcg cttacgtaac ttgtaactag ctgcctagtt
      721 tgatctttgc ttcttcattg attgcagtag atttcaaaat gataaaaacg cataatagtg
      781 ggtgtagaaa ctcttgttac tatgcgtttt gttatggaaa gacttattgg actttctctc
      841 aaatcgagtt tttactcaat tttcttactt gattgggatt gaaattccaa ttaatttctc
      901 tgagtagagt gtcttgatat tggcttcatc aacagaggtc ttatcaattt tacgtttcaa
      961 gaaaaattct tgaatggttt cgatttcagg ctcacgaata gcacggtgtt tgtttgagat
     1021 gaggatttca tagtgaagcg gagcttgggt aaaaataaca tctgtattcc ctgcagaata
     1081 aacctcaaca agggttgcat cggtactttc tagctgactt tttacaagtt gcgagtgtga
     1141 gtttgtcgta ttgataagct tcataatatt tcctccggtt ttctaattct attatagcac
     1201 tttttgaata aagtcgcttg atttatactc aatgaaaata aaagagcaaa ctaggaagct
     1261 agccgcaggc tatacttgag tatggtaagg cgacgctgat gtggtttgaa ttttattttc
     1321 gaagagtatt agtcaatctt atgctgtttt ttccaagatt caatggccca tttatggcta
     1381 ccacgtttaa ggtttttgat agcctcgtca atagggaacc aggcaatatg attaaagttt
     1441 tctagtggct tttgtacttc tttgaaagga gttgcttcat agaggtaggc aggattggag
     1501 tagtaggtat cacgatgacg agaatagaaa tattcgtcag cttgtccgta ataggtacca
     1561 atttctgctg tgaaaccaag ctcttcaatc aactcacgct ttagggcttc ctgatgattt
     1621 tcacctgctt caatttctcc acatggtagg aaccaagcac catttggttc ttgaacaaga
     1681 acaatttgtt tttgttcagg attagggata actgcatata cgccatagcg agcaatatag
     1741 tctgtattcg cttttttctc cgaaagttgg gtttgccatt gcattttcct cattatctag
     1801 tatcgttatt attatagtga aatgaaccaa aaatagtaca caatgtggta taatcttctt
     1861 atggcatatt caatagattt tcgtaaaaaa gttctctctt attgtgagcg aacaggtagt
     1921 ataacagaag catcacacgt tttccaaatc tcacgtaata ccatttatgg ctggttaaag
     1981 ctaaaagaga aaacaggaga gctaaaccac caagtaaaag gaacaaaatc aagaaaagtt
     2041 gatagagata gacttaaaaa ctatcttact gacaatcccg acgcttattt gactgaaata
     2101 gcttctgact ttggctgtca tccaactacc atccactatg cgctcaaagc tatgggctac
     2161 actcgaaaaa aagaaccaca cctactatga acaagagcca gaaaaagtag ccttatttct
     2221 taagaatttt aatagtttaa agcacctagc acctgtttag attgatgaaa caggattcga
     2281 tacttatttt tatcgagaat atggtcgctc attaaaaggt cagttaagaa gaggcaaagt
     2341 atctggaaga agatatcaga ggatttcttt ggttgcaggt ctaacaaatg gtgagttaat
     2401 cgttccaatg acttacgaag agacgatgac gagcgacttt tttgaagctt ggtttcagaa
     2461 gtttttctta ccaacattaa ccacaccatc ggttattatt atggataatg taagattcca
     2521 tagaatgggg aagctagaac tcttgtgtga agagtttggg cataaacttt tacctcttcc
     2581 tccctactca cctgagtaca atcctattga gaaaacatgg gctcatatca aaaagcacct
     2641 caaaaaggta
//
LOCUS       DAVLVP010000141         2611 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.141, whole genome shotgun sequence.
ACCESSION   DAVLVP010000141 DAVLVP010000000
VERSION     DAVLVP010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2611)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2611)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2611
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.141"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..201)
                     /locus_tag="VYN69_001950"
     CDS             complement(<1..201)
                     /locus_tag="VYN69_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001810555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534159.1"
                     /translation="MSEVDFNEAVNYEFTSDTCQLANSIYQSLFKFFDKKNFSGDLIF
                     TWKSPSLVKEGDYIGRRDSQVDN"
     gene            complement(259..972)
                     /locus_tag="VYN69_001951"
     CDS             complement(259..972)
                     /locus_tag="VYN69_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEV1534160.1"
                     /translation="MFYKFLLFDLDHTLLDFDAAEDVALTQLLKEEGVADIQAYKDYY
                     VPMNKALWKDLELKKISKQELVNTRFSRLFAHFGQEKDGSFLAQRYQFYLAQQGQTLS
                     GAHDLLDSLIGRDYNLYAATNGITAIQTGRLAQSGLAPYFNQVFISEQLQTQKPDALF
                     YEKIGQQIAGFSKEKTLMIGDSLTADIQGGNNAGIDTIWYNPHHLENHTQAQPTYEVY
                     SYQDLLDCLDKNILEKITF"
     gene            complement(1431..1811)
                     /locus_tag="VYN69_001952"
     CDS             complement(1431..1811)
                     /locus_tag="VYN69_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000443785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEV1534161.1"
                     /translation="MESIGIGLVIVSHSKHIAEGVVELISKVAKDVPITYVGGTEGGG
                     IGTSFDQVDRVVSENPADTLLAFFDLGSAKMNLKMVTDFSDKSIIINRVPIVEGAYNA
                     AALLQAGAELSVIQIQLAELEINK"
     gene            complement(2233..>2611)
                     /locus_tag="VYN69_001953"
     CDS             complement(2233..>2611)
                     /locus_tag="VYN69_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidine motif-containing protein"
                     /protein_id="HEV1534162.1"
                     /translation="RNKNGQADTNQTEKPSEEKPQTEKPEEETPREEKPQSEKPESPK
                     PTEEPEESPEESEEPQVETEKVEEKLREAEDLLGKIQDPIIKSNAKETLTGLKNNLLF
                     GTQDNNTIMAEAEKLLALLKESK"
BASE COUNT          790 a          516 c          417 g          888 t
ORIGIN      
        1 attatctact tgtgaatccc ttctcccaat ataatcccct tctttgacta atgatggaga
       61 tttccaagta aaaattaaat cgccagagaa atttttctta tcaaaaaact taaaaagaga
      121 ttgataaata ctatttgcta attgacaagt gtcagatgta aattcataat taacagcttc
      181 attaaaatca acttcagaca tttcaatgta ctcttcaata gtaatattag cttttttgta
      241 gtcattagct cgtctccttt aaaatgtgat cttttcaaga atatttttat ctaaacaatc
      301 cagcaagtct tggtaagaat agacttcgta agtcggctgg gcttgtgtgt gattttcgag
      361 gtgatgagga ttataccaga tagtgtcaat ccccgcatta ttgccacctt gaatgtcggc
      421 ggttagagaa tctccaatca tcagcgtctt ttctttacta aatccagcaa tttgctggcc
      481 aatcttttca taaaaaagag catccggctt ttgagtttgc aactgttctg agataaagac
      541 ttgattgaaa taaggtgcta gaccagattg agccaaacgt cctgtctgaa tggcagtaat
      601 gccatttgtc gcagcataca agttataatc acgcccaatg aggctgtcca agagatcatg
      661 agcgcccgat agtgtttgtc cctgctgggc gaggtaaaat tggtaacgct gggcaagaaa
      721 actaccgtct ttttcttgtc caaaatgagc aaataaacga gaaaagcgcg tgttaaccag
      781 ctcttgttta ctgattttct tcagctccaa gtctttccag agagccttgt tcataggaac
      841 gtaataatct ttataagcct gaatatccgc aactccttct tcttttagaa gttgggtcaa
      901 agccacatcc tcagcagcat caaaatcaag aagagtgtgg tcgaggtcga agagtagaaa
      961 tttgtagaac aatttgaggt tttcctttct gaaaattcat taagaacatt atatcataaa
     1021 gcacctcata caattaacta atttaatcac ttaaaaaaaa ttcgaacact ttctatacaa
     1081 ctgacagctc aaatctttca gaatagaaca atactaacta tcgaacaccc tgtcttcata
     1141 aatacatatg taattctagg cctagaattc ctataaacta aatgctttca tactcttcca
     1201 agtaattgat tgccttaaat tttaattttt gaaggtttct aaagctagaa tagccccatc
     1261 acaatcagtt ttgattgatt cacaatttag aaacactata gtttcactcc tgttaaaata
     1321 aaaaggaact gcataaagca atccctttct gattttgaaa tcatttactt aacattttat
     1381 agttgagata atcaatagct tatctataaa aagagttata gtaaaattcc ttatttattg
     1441 atttcaagct ccgctaactg tatttgaata actgacagtt ctgcaccagc ctgaagaaga
     1501 gcagctgcat tataggcacc ttctacaatt ggaaccctgt tgatgatgat acttttatca
     1561 ctgaaatcag tcaccatttt taagttcatt ttagcagaac ctaggtcaaa aaaggcaagt
     1621 aaagtatctg ctggattttc ggaaacaacc ctatctactt gatcaaaact cgttccaatt
     1681 cctccgccct cggttcctcc tacataagta atcggaacat ctttagctac tttactaatc
     1741 agttcaacaa caccttctgc aatgtgtttg gaatgtgaaa cgataacaag accaatacca
     1801 atactttcca tcaaaccact ccagtttcta aaatagcagt aaagagtaat cctgatgaga
     1861 atgatccagg atcaatatgt ccaagaaacc acatgctcct aagacaagag ctaacagact
     1921 ggccatcaat aatagtattg ttcttttttt catcattact ccttaactag tgtttaactg
     1981 attaattagc cagtaaatag tttatcttta tttacactat ctgttaagat atagtaaaat
     2041 gaaataagaa caggacagtc aaatcgattt ctaacaatgt tttagaagta gaggtatact
     2101 attctagttt caatctacta tattttgcac attttcatga aaaaaatgag aactagaact
     2161 cacattctgc tctcattttt cgttttcccg ttctcctatc ctgtttttag gagttagaaa
     2221 atgctgctac ctttacttac tctcctttaa taaagccaat agtttttcag cttctgccat
     2281 aatagtattg ttgtcctggg tgccaaatag taaattattt tttaatcctg tgagagtctc
     2341 tttggcattg gacttgataa ttggatcctg gatttttcca agtaaatctt cagcctctct
     2401 cagtttttct tcaacctttt cagtctcgac ctgaggttct tctgattcct ctggtgattc
     2461 ttctggttcc tctgttggtt ttggagactc tggtttctcg ctttgcggtt tctcttctcg
     2521 aggggtttct tcctcaggtt tttctgtctg aggtttctcc tcgcttggtt tttccgtttg
     2581 attggtatca gcttgaccat ttttgtttct t
//
LOCUS       DAVLVP010000142         2602 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.142, whole genome shotgun sequence.
ACCESSION   DAVLVP010000142 DAVLVP010000000
VERSION     DAVLVP010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.142"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            239..2563
                     /gene="pflB"
                     /locus_tag="VYN69_001954"
     CDS             239..2563
                     /gene="pflB"
                     /locus_tag="VYN69_001954"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016399021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEV1534163.1"
                     /translation="MVVKTVVEAQDIFDKAWEGFKGVDWKEKASVSRFVQANYTPYDG
                     DESFLAGPTERSLHIKKIVEETKAHYEETRFPMDTRPTSIADIPAGFIDKENEVIFGI
                     QNDELFKLNFMPKGGIRMAETTLKENGYEPDPAVHEIFTKYVTTVNDGIFRAYTSNIR
                     RARHAHTVTGLPDAYSRGRIIGVYARLALYGADYLMQEKVNDWNAIKEIDEETIRLRE
                     EVNLQYQALQQVVRLGDLYGVDVRKPAMNVKEAIQWVNIAFMAVCRVINGAATSLGRV
                     PIVLDIFAERDLARGTFTESEIQEFVDDFVMKLRTVKFARTKAYDQLYSGDPTFITTS
                     MAGMGNDGRHRVTKMDYRFLNTLDNIGNSPEPNLTVLWTDKLPYNFRRYCMHMSHKHS
                     SIQYEGVTTMAKDGYGEMSCISCCVSPLDPENEEQRHNIQYFGARVNVLKALLTGLNG
                     GYDDVHKDYKVFDIEPIRDEVLEFESVKANFEKSLDWLTDTYVDALNIIHYMTDRYNY
                     EAVQMAFLPTKQRANMGFGICGFANTVDTLSAIKYATVKPIRDEDGYIYDYETIGDYP
                     RWGEDDPRSNELAEWLIEAYTTRLRSHKLYKDAEATVSLLTITSNVAYSKQTGNSPVH
                     KGVYLNEDGSVNLSKLEFFSPGANPSNKAKGGWLQNLNSLSSLDFSYAADGISLTTQV
                     SPRALGKTRDEQVDNLVTILDGYFENGGQHVNLNVMDLNDVYEKIMSGEDVIVRISGY
                     CVNTKYLTPEQKTELTQRVFHEVLSMDDALDALS"
BASE COUNT          824 a          544 c          508 g          726 t
ORIGIN      
        1 ttcgatttcc attgagtgtt actgcttatt ttcttttatt ataccctttt ttctgaaaaa
       61 aagaaaaaag gactttattt tttcaaaaat ataatacagt ttgaaataaa atatagactg
      121 ttttagaaaa gaaagtgtaa aaatagggaa ttttcacttg ttgaaatcgg ttactatatg
      181 gtatacttgt cttatgaatg taacagatga ctgttactag aaaaaagagg acattaatat
      241 ggttgttaag acagttgttg aagcacaaga tatttttgac aaagcttggg aaggcttcaa
      301 aggcgtagat tggaaagaaa aagcaagtgt atcacgattt gtacaagcta actacacacc
      361 ttatgatgga gacgaaagct tccttgcagg accaacagag cgttcacttc acatcaagaa
      421 aattgtagaa gaaactaaag cacactacga agaaactcgt ttcccaatgg acactcgtcc
      481 aacatctatc gctgatatcc ctgctggatt tatcgacaaa gaaaatgaag ttatctttgg
      541 tatccaaaat gatgaactct tcaaattgaa cttcatgcca aaaggtggta tccgtatggc
      601 tgaaactact ttaaaagaaa atggatacga accagaccca gctgttcacg aaatcttcac
      661 taaatatgta acaacagtta acgacggtat tttccgtgcc tacacttcaa atattcgtcg
      721 cgctcgtcat gcacacactg taactggtct tccagatgca tactcacgcg gacgtatcat
      781 cggtgtttac gcacgtcttg ctctttacgg tgcagactac ttgatgcaag aaaaagtaaa
      841 cgactggaat gcaatcaaag aaatcgatga agaaacaatc cgtcttcgtg aagaagtaaa
      901 ccttcaatac caagcattgc aacaagttgt tcgcctgggt gacctttacg gggttgatgt
      961 tcgcaaacca gcgatgaacg tgaaagaagc aatccaatgg gttaacattg ctttcatggc
     1021 tgtctgccgt gtgattaacg gtgctgctac atctctaggt cgtgtaccaa tcgtattgga
     1081 catctttgca gaacgtgacc ttgctcgtgg tacatttact gaatcagaaa tccaagaatt
     1141 cgttgatgat ttcgttatga aacttcgtac agttaaattt gctcgtacaa aagcttatga
     1201 ccaattgtac tcaggtgacc caacctttat cacaacttct atggctggta tgggtaacga
     1261 cggtcgtcac cgtgttacta agatggacta ccgtttcttg aacacacttg acaacatcgg
     1321 taactcacca gaaccaaact tgacagttct ttggactgac aaattgccat acaacttccg
     1381 tcgctactgt atgcacatga gccacaaaca ctcttctatc caatacgaag gtgtaacaac
     1441 aatggctaaa gacggatatg gtgaaatgag ctgtatctca tgctgtgtgt ctccacttga
     1501 tccagaaaat gaagaacaac gccacaacat ccagtacttc ggtgctcgtg taaacgttct
     1561 taaagccctt cttactggtt tgaatggtgg ttacgacgat gttcacaaag actacaaagt
     1621 atttgatatc gaaccaatcc gtgacgaagt tcttgaattc gaatcagtta aagcgaactt
     1681 tgaaaaatct cttgactggt tgactgacac ttacgtagat gccttgaaca tcatccacta
     1741 catgactgat aggtacaact acgaagctgt tcaaatggcc ttcttgccaa ctaaacaacg
     1801 tgccaacatg ggattcggta tctgtggatt tgctaacact gttgatacat tgtcagctat
     1861 caaatacgct acagttaaac caatccgtga cgaagatggc tacatctacg attacgaaac
     1921 aatcggtgac tacccacgct ggggtgaaga tgacccacgt tcaaacgaat tggcagaatg
     1981 gttgatcgaa gcttacacaa ctcgtctacg tagccacaaa ctatacaaag acgcagaagc
     2041 tacagtatca cttttgacaa tcacatctaa cgttgcttac tctaaacaaa ctggtaactc
     2101 accagttcac aaaggtgtat acctcaacga agatggttct gtgaacttgt ctaaacttga
     2161 attcttctca ccaggtgcta acccatctaa caaagctaaa ggtggttggt tgcaaaactt
     2221 gaactcactt tctagccttg actttagtta tgcagctgac ggtatctcat tgactacaca
     2281 agtatcacct cgcgctcttg gtaagactcg tgatgaacaa gttgataact tggtaacaat
     2341 ccttgatggt tacttcgaaa acggtggaca acacgttaac ttgaacgtta tggacttgaa
     2401 cgatgtttac gaaaaaatca tgtcaggcga agacgttatc gtacgtatct ctggatactg
     2461 tgtaaacact aaatacctca ctccagaaca aaaaactgaa ttgacacaac gtgtcttcca
     2521 cgaagttctt tcaatggatg acgccttgga tgcattgagc taatcaagtt cggctctttg
     2581 tcaactgcag tgggttgaag tc
//
LOCUS       DAVLVP010000143         2529 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.143, whole genome shotgun sequence.
ACCESSION   DAVLVP010000143 DAVLVP010000000
VERSION     DAVLVP010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.143"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..157
                     /locus_tag="VYN69_001955"
     CDS             <1..157
                     /locus_tag="VYN69_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000176371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEV1534164.1"
                     /translation="QETLDKHHQSKTMQKILDLRKKYHLQMQVERYIEDDSGMPESDK
                     SFIDTGN"
     gene            347..1783
                     /locus_tag="VYN69_001956"
     CDS             347..1783
                     /locus_tag="VYN69_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEV1534165.1"
                     /translation="MTTFEDGFLWGGAVAAHQLEGGWQEGGKGISVADVMTAGRHGVA
                     REITSGVLEGKYYPNHEAIDFYHRYKEDIALFAEMGFKCFRTSIAWTRIFPKGDELEP
                     NEEGLQFYDNLFDECLKNGIEPVITLSHFEMPYHLVTEYGGWKNRKLIDFFARFAEVV
                     FKRYKDKVKYWMTFNEINNQANYKEDFAPFTNSGIVYEEGDNREAIMYQAAHYELVAS
                     ARAVKIGHEINPDFQIGCMIAMCPIYPATCNPKDILMAMKAMQKRYYFADVHVLGKYP
                     EHIFKYWERKGISVDFTAQDKEDLLGGTVDYIGFSYYMSFAIDSHRENNPYFDYLETE
                     DLVKNNYVKASEWEWQIDPEGLRYALNWFTDHYHLPLFIVENGFGAIDQVAADGMVHD
                     DYRIEYLGAHIREMKKAVVEDGVDLMGYTPWGCIDLVSAGTGEMRKRYGFIYVDKDDN
                     GKGSYNRSPKKSFGWYKEVISSNGESVE"
     gene            1835..2074
                     /locus_tag="VYN69_001957"
     CDS             1835..2074
                     /locus_tag="VYN69_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000580385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SemiSWEET family sugar transporter"
                     /protein_id="HEV1534166.1"
                     /translation="MIGSIAAILTTFAFLPQVFRVVKTKDTGSIALGMYVMQVIGIAL
                     WLAHGIRIGDLPLILANSVSFLLSGIILFYKLKYK"
     gene            2204..2518
                     /locus_tag="VYN69_001958"
     CDS             2204..2518
                     /locus_tag="VYN69_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003037418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEV1534167.1"
                     /translation="MAKALIICAAGMSSSLMAQKTTEFLKGKGKDIVVDAISATEGGK
                     AIAAAAFDLYLVSPQTKMYFKQFEEAGSKAGKPVVQIPPQAYIPIPMGIEKMANLILE
                     NI"
BASE COUNT          829 a          329 c          537 g          834 t
ORIGIN      
        1 tcaagaaact ttagataagc atcatcaatc aaagacaatg cagaaaatct tagatttaag
       61 aaaaaaatat cacttgcaga tgcaagttga acgttatata gaagatgatt ctgggatgcc
      121 agaaagcgat aagtcgttta tagacacagg gaattagaat tattgtgaga gtttttgtag
      181 tttgaaagta ttgaaaatcg taaagatatc attacgagaa tacaagaaga tattgttcta
      241 tttccattaa aaattacttc tttccttaac aataaggaaa aagaatgcgt tttcatcttt
      301 tggtttgagg tttataataa gagtatcaaa gtcaggagga aaaaagatga ctacttttga
      361 agatggattt ttatggggtg gtgctgttgc tgctcatcaa cttgaaggcg gatggcaaga
      421 aggtggcaaa ggaattagtg ttgctgatgt tatgactgct ggtcgccatg gagtagctcg
      481 tgaaattact tcgggagttt tagagggtaa atattatcca aatcatgagg cgatagattt
      541 ttatcatcgt tataaagaag acatagcact ttttgctgaa atgggattca agtgcttccg
      601 tacctctatt gcatggacac gtatctttcc aaaaggtgat gagttagagc cgaatgaaga
      661 aggattacag ttttatgata atctttttga tgaatgctta aagaatggta ttgaacctgt
      721 catcactcta tctcattttg aaatgcctta tcacttagtg accgaatatg gtggttggaa
      781 aaataggaaa ttgattgatt tctttgctcg ttttgcagaa gtcgtattta aacgttacaa
      841 agataaggtt aaatattgga tgactttcaa tgaaatcaat aatcaagcga attataagga
      901 agattttgca ccatttacta actcaggtat tgtatatgag gaaggtgata atagagaggc
      961 aattatgtat caagcagcac attacgaatt agttgcttct gcacgagctg taaaaattgg
     1021 tcatgagatt aatccagatt ttcaaatagg ttgtatgatt gcgatgtgtc caatttatcc
     1081 agctacttgc aatcctaagg atatcttaat ggcaatgaaa gctatgcaga agcgttatta
     1141 ttttgctgat gtgcatgttt taggtaaata tcctgagcat attttcaagt attgggaacg
     1201 aaaaggtatt tcagttgatt ttactgccca ggataaagaa gatttacttg gtgggactgt
     1261 agattacatt ggtttcagtt actatatgtc ctttgctatc gactctcatc gtgaaaataa
     1321 tccttatttt gattatcttg aaacggaaga tttagtgaaa aataattatg ttaaggcttc
     1381 tgaatgggag tggcaaattg atccagaagg tttgcgttat gcgttaaatt ggtttacaga
     1441 ccactatcac ttaccactct ttattgttga aaatggtttt ggagctatag atcaagttgc
     1501 agcagatggt atggtacatg atgattatag aattgaatat ctaggtgccc atattcgtga
     1561 aatgaaaaag gctgtagttg aagatggtgt tgatttaatg ggttatactc catggggatg
     1621 tattgatttg gtttcagctg gtaccggtga aatgcggaaa cgttatggct ttatttatgt
     1681 agataaagat gacaatggga agggaagtta taatcgttcc ccgaaaaaat cttttggctg
     1741 gtataaggaa gttatttcat ctaacggtga atcagtagaa tagtataaac aaactgagat
     1801 tattctcagt ttgtttagca tattgaggtg acatatgatt ggaagtattg cagcaatttt
     1861 aacaacattt gcatttttac cacaagtttt tcgtgtagtt aagacaaagg acacaggatc
     1921 tattgctcta ggtatgtatg ttatgcaagt tattggtatt gcattgtggc ttgctcatgg
     1981 tattcgtata ggagacttgc ctttgatttt agcaaatagt gtttcatttc tactctcagg
     2041 aataatttta ttttataaat tgaagtataa ataatttttt tccatatcat ttaggaaaat
     2101 gagggaattg tcattggaaa cgctttattt tataataaaa atagataaca aataagccaa
     2161 cttagcacag ctttgattgg atttaagaaa aggagaaaat attatggcaa aagctttaat
     2221 tatttgtgca gcaggaatgt cttcatcact tatggcacag aaaacaacag agtttctaaa
     2281 aggaaaaggg aaagatattg tagttgatgc tattagtgct actgaaggag ggaaagctat
     2341 cgcagcagca gcttttgatt tgtatcttgt cagccctcaa acaaaaatgt actttaagca
     2401 atttgaagag gctggttcta aagcaggaaa accagttgta caaatacctc ctcaagccta
     2461 tattcctatt ccaatgggaa ttgaaaaaat ggctaatttg attcttgaga atatttgatg
     2521 aacacatga
//
LOCUS       DAVLVP010000144         2405 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.144, whole genome shotgun sequence.
ACCESSION   DAVLVP010000144 DAVLVP010000000
VERSION     DAVLVP010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2405)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2405)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2405
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.144"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..154
                     /locus_tag="VYN69_001959"
     CDS             <1..154
                     /locus_tag="VYN69_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000756162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type I restriction endonuclease subunit S"
                     /protein_id="HEV1534168.1"
                     /translation="KKILLPLPPLALQNEFADFVAQVDKSQLAIQKSLEELETLKKSL
                     MQEYFG"
     gene            214..450
                     /locus_tag="VYN69_001960"
     CDS             214..450
                     /locus_tag="VYN69_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000260605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEV1534169.1"
                     /translation="MVVKTRKQGNSITITIPSEFNIPSGVKYEAKLLPSGEIIFTPEE
                     LGQQVSYVSDDAFDLNLDKIFDEYDDVFKALVEK"
     gene            447..860
                     /locus_tag="VYN69_001961"
     CDS             447..860
                     /locus_tag="VYN69_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000158364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEV1534170.1"
                     /translation="MTIYLTEKQIEKINALAIQRYSPNEKIQTVSPSALNMIVNLPEQ
                     FVFGKPLYPTIFDKATILFVQLIKKHVFANANKRTAFFVLVKFLQLNGYRFSVTVEEA
                     VKMCVTIAVEALTDEKMTSYSKWISEHSVREKVKK"
     gene            978..1943
                     /locus_tag="VYN69_001962"
     CDS             978..1943
                     /locus_tag="VYN69_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1534171.1"
                     /translation="MESKVTIIMQEMLPLLNNEQLLALRESLEHHLVDGKKQQKYSNN
                     NLLQLFITAKQVEGCSSKTIRYYQRTIENLFNAIKESVTQLTTDDLRSYLANYQSEKD
                     CSKANLDNIRRILSSFFAWLEQEEYIIKNPIRRIKKIKTEQNVKETYTDEHLEIMRDN
                     CENLRDLAIIDLLASTGMRVGELVQLNRSDIDFENRECVVFGKGKKERPVYFDARTKI
                     HLRNYLNDRKDSHPALFVTLVGKVQRLGIAGVEIRLRKLGDKLGIQKVHPHKFRRTLA
                     TKAIDKGMPIEQVQKLLGHSKIDTTLAYAMVNQNNVKHSHQKFIS"
     gene            1909..>2405
                     /locus_tag="VYN69_001963"
     CDS             1909..>2405
                     /locus_tag="VYN69_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001852328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEV1534172.1"
                     /translation="MSSIHTKNSSLKSKSRFNEMFGGNKIFESIDNLFDIIDGDRGKN
                     YPKSDELFSEEYCLFLNTKNVTKNGFSFDTKQFITKTKDKLLRKGKLERYDIVLTTRG
                     TVGNVAYYDELIKYKHLRINSGMVILRPKTPNLNQKFIIHVLRNNNYSRVISGSAQPQ
                     LPITK"
BASE COUNT          886 a          361 c          454 g          704 t
ORIGIN      
        1 aaaaaaaata cttctccccc tccccccact agccctccaa aatgagttcg cagactttgt
       61 agcccaggtc gacaaatcac aattggcaat ccaaaaatct ctggaagaac ttgaaacttt
      121 gaagaaatct ctgatgcagg agtattttgg ctgatattct gccattgtaa ttacggtaat
      181 gatttgttat aatacttcaa aggaggaaat cagatggtag taaaaacaag aaaacaagga
      241 aattcaatca ccattacgat tccaagtgaa tttaatattc caagtggtgt taaatacgaa
      301 gcgaaattgt taccaagtgg tgagattatc tttactcctg aagaattggg gcagcaggtt
      361 tcttatgtat ctgatgatgc ctttgactta aatttagata aaatatttga cgaatacgac
      421 gatgttttca aagctttggt ggaaaaatga caatctattt gacagaaaag caaattgaaa
      481 aaataaatgc tttagcaatt caacggtatt ctccaaatga gaaaattcaa acagttagtc
      541 cttctgcctt aaatatgatt gtgaacttac cagaacaatt tgtctttggg aagcctcttt
      601 atccaacaat ttttgataaa gcaacgatac tatttgtcca attgataaag aagcatgttt
      661 ttgctaatgc taataaaaga actgctttct tcgttttggt caaattttta caattaaacg
      721 gttatcgttt ttctgtaacg gtagaagaag cagtaaaaat gtgtgtaacc atcgcagtag
      781 aagctttaac tgatgaaaaa atgacaagct actccaaatg gatttctgaa cattctgtta
      841 gagaaaaggt caaaaagtaa cctagtatgc tggatttgaa tgagcacaag aaaataaatg
      901 aacagacaat attagaattc tgtaatgcag aaactgatat tgtctctttt tattgatgaa
      961 taagaaagtg agaaattatg gaatcaaaag ttacaattat catgcaagaa atgttacctc
     1021 ttttaaataa tgaacaatta ctagcgttga gagagagttt agaacatcat ctagtagacg
     1081 gaaaaaagca gcagaagtat tcgaataata acctgttgca actatttatt accgccaagc
     1141 aggtagaggg ctgtagctca aaaacaattc gttattatca gaggacgatt gaaaacttgt
     1201 ttaatgctat taaagagtct gtgacacaac tcacaacaga tgatttaagg agttatttag
     1261 caaattacca gtctgaaaag gattgtagta aggcaaattt agacaatatt aggcgtatat
     1321 tgtcttcttt ttttgcttgg cttgagcaag aggaatatat cattaaaaat cccattcgac
     1381 ggataaagaa aattaagact gagcaaaatg tgaaggaaac ttatactgat gaacatttgg
     1441 aaattatgcg tgataactgt gaaaatttga gagatttggc aataatagac ctactagcat
     1501 cgacaggtat gcgtgtaggg gagcttgtac agttgaatcg ttcagatatt gattttgaaa
     1561 acagagagtg tgttgtcttt ggtaaaggaa agaaggagag accagtatat tttgacgctc
     1621 gtacgaaaat tcatttaaga aattatctta acgacagaaa agatagtcac cctgctcttt
     1681 ttgtaacgct agttggaaaa gtccagaggc ttggaattgc tggtgtagag attcgcttaa
     1741 gaaagttagg agacaaactc ggcatacaaa aggttcaccc acataagttc agaagaactt
     1801 tagcgactaa ggcaattgat aaaggtatgc ctatcgaaca agtccaaaaa ctgctaggtc
     1861 atagcaagat tgacacaacc ctggcctatg ccatggtcaa tcaaaataat gtcaagcatt
     1921 cacaccaaaa attcatctct taaaagcaaa tcccgattta acgagatgtt tgggggaaat
     1981 aaaatatttg aaagcattga taacttattt gatattatag atggtgatag gggcaaaaat
     2041 tatcctaaat cagatgagtt gtttagtgag gagtactgtt tatttttaaa tacaaagaat
     2101 gttactaaaa acggattttc attcgataca aagcaattta tcactaaaac aaaggataaa
     2161 ttacttcgaa aaggcaaact tgagcgttat gatatagtct tgacaacaag aggtactgtt
     2221 ggaaatgtag cgtactacga tgaattaata aaatataaac atttacgtat aaattcaggt
     2281 atggtaatat tacgtcccaa gacaccaaat ctaaatcaga aatttattat ccatgtttta
     2341 aggaataata attatagtcg agtgatatca ggaagtgctc agcctcagtt accaattaca
     2401 aaatt
//
LOCUS       DAVLVP010000145         2377 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.145, whole genome shotgun sequence.
ACCESSION   DAVLVP010000145 DAVLVP010000000
VERSION     DAVLVP010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2377
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.145"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            214..1360
                     /locus_tag="VYN69_001964"
                     /pseudo
     CDS             214..1360
                     /locus_tag="VYN69_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611423.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(1476..2279)
                     /locus_tag="VYN69_001965"
     CDS             complement(1476..2279)
                     /locus_tag="VYN69_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001866401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family protein"
                     /protein_id="HEV1534173.1"
                     /translation="MQSTYNIDNPNLSYEAKRDLWRIGFGLQKVDNLVPSAYMESLAE
                     KQSRGELTYEQVYEDATAYHHTIDASTEEADLVSLRIVELLSRRGFSFSPATLLAIHK
                     ELFQDIFEPSIPVGQFRQTNITKNEPVLNGESVVYSDYSMIQMTLDYDFNQEKQVAYA
                     TLTQADVVKQIQHFISGIWQIHPFREGNTRTVTVFLIQYLREFGFDIDNTPFQQHSKY
                     FRDALVLDNAKILQRRSEFLTAFFENLLLGGQNDLSSEKMYLDLDLDFS"
BASE COUNT          836 a          426 c          431 g          684 t
ORIGIN      
        1 ctatttttga ttgagtaaaa gacactttaa ttttctggct cttcaactgt gaactacacc
       61 ccaaaagtta gacagaaaaa atctaacttt tggggtgttt tattatgaaa ctaacttatg
      121 atgataaagt tcagctatga acttagaaaa caaggatata gcttagagaa gctttcaaat
      181 aaatttggga taaacaattc taatcttagg tacatgatta aattgattga tcgttacgga
      241 atagagttcg tcaaaaaagg aaaaaatcgt tactattctc ctgatttaaa acaagaaatg
      301 attaataaag tctgacatga aggctggact aaagatagag tttctcttga atactgcctc
      361 ccaagtcgta cgatacttct taactggcta gcacaataca ggaaaaacgg gtatactatt
      421 gttgagaaaa caagagagag tacctgagag cggagaatgc catcctaaaa aagttaagag
      481 aactccgatt gaaggaggaa aaagagaaag aagaaagaca gaaattgttc aagaattaat
      541 gactgagttt tcgttagatc ttcttctaaa agccattaaa ctagctcgtt ggacctacta
      601 ctatcacttg aaacagctag acaaaacaga taaggaccaa gagcttaaag ctgaaattca
      661 atccattttt atcgaacata agggaaatta tgcttatcgt cggatttatt tagaactaag
      721 aaatcgtggt tatctggtaa atcataaaag agttcaaggt ttgatgaaag tactcaattt
      781 acaagctaaa atgcgacaga aacgaaaata aaggagacgt tggtaagaag gcagagaatc
      841 tcattcaagg ccaatttgaa ggctctaaaa caatggaaaa gtgctacaca gatgtgactg
      901 aatttgccat tccagcaagt actcaaaagc tttacttatc accagtttta gatggcttta
      961 acagcgaaat tatcgcctat aatctttcaa cttcacccaa cttagaacaa gtacaaacaa
     1021 tgttggaaca ggcattcaca gagaagcact acgagaatac gattctccat agtgaccaag
     1081 gctggcaata ccgacacgat tcttatcatc ggttcctaag agtaagggaa ttcaagcatc
     1141 tatgtcacgc aagggaaaca gccaagacaa cggtatgatg gagtccttct ttgggattct
     1201 gaaatcggaa atgttttacg gttatgagaa gtcgtttcag tcgcttaagc aattggaaca
     1261 agctattgta gactatattg attactacaa caataaacga attaaggtaa aactaaaagg
     1321 acttagcctt gtgcaataca gaactaaatc cttcggataa attatttgtc taactttttg
     1381 gggtcagtac acctaaaact ttgatgatat acgttttctt gtgagaatat ttacttcatt
     1441 tttgcctaaa attcaatgtt tactcagtat ttggattatg aaaaatcgag gtctaaatct
     1501 agatacattt tttctgaaga caaatcattt tgaccaccga gcaagagatt ttcaaaaaaa
     1561 gctgttaaaa actcagaacg tcgctgtaaa atctttgcat tatctaatac caaggcatca
     1621 cgaaaatatt tggaatgttg ctgaaatggt gtattatcaa tatcaaaacc aaactcacga
     1681 agatactgaa tcaaaaagac cgttactgtc cgagtgtttc cttcgcgaaa tggatgaatc
     1741 tgccagattc ctgaaataaa atgctggatt tgtttaacca catccgcctg agttagtgtc
     1801 gcatatgcaa cttgtttttc ctgattaaaa tcataatcta aggtcatttg aatcatggag
     1861 taatcagagt acacaacact ttcaccattc aaaacaggtt cattctttgt gatattggtc
     1921 tgacgaaatt gccccaccgg aatagagggt tcaaatatat cttgaaacaa ctccttatga
     1981 atagcaagta aggtcgcagg actaaagcta aagcctcttc gagacaatag ttctacaata
     2041 cgtagagaaa ccaagtctgc ctcttccgta cttgcatcaa tagtatggtg ataagccgtt
     2101 gcatcctcat aaacctgctc ataagtcagt tctccccggg actgtttctc agccaaagat
     2161 tccatatacg ctgatggcac tagattgtca actttctgca gaccaaaacc tatccgccat
     2221 aaatcacgct tcgcttcata agacaagttt ggattgtcaa tgttgtaagt tgattgcata
     2281 gaaatatcct ttcaattgtt tttcttcatt accagtaaat cacgaaattg gttggaaact
     2341 ggcttaaact ataaaaagtc ctcacaatag gttctat
//
LOCUS       DAVLVP010000146         2364 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.146, whole genome shotgun sequence.
ACCESSION   DAVLVP010000146 DAVLVP010000000
VERSION     DAVLVP010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.146"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            1..596
                     /locus_tag="VYN69_001966"
                     /pseudo
     CDS             1..596
                     /locus_tag="VYN69_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001817814.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="lanthionine synthetase"
     gene            639..1871
                     /locus_tag="VYN69_001967"
     CDS             639..1871
                     /locus_tag="VYN69_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000765682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1534174.1"
                     /translation="MKLFWTNNIYRQLLLNSCFSSFGDSIFYLAIINYVAQYNFAPLA
                     ILLISISEMVPLLSQLFLGILGDFQENRVKHALCIAKIKILLYAILTVFLVLSPFSLV
                     SVIMIVIINLISDTLSYLSAYMMNALYISVIKDNLHDAMGFRQSLMRVVRIVANLAGV
                     FLINVMSIQTISLINTLTFVIAFLGLYVIRHTLYEVEKKIEMSHTALSFKKYFQHLKQ
                     SLAVLLRLKDTVILLFLTTSMIAILDVSPRLIALRFIQQTLAQLSIGQLLALLSIIIS
                     CGAILGNMTSSNLFKNIRFTHLLVFCEISLLALITSILCQTYIVIFMTSFISSTIIGI
                     LSPRLQAAVFAHIPSDKMGTVGSALSTVDILAPSLLSLLALSIASGVSVQLALIFLYL
                     ILIALIFCQWLVTFNTHN"
     gene            2135..>2364
                     /locus_tag="VYN69_001968"
     CDS             2135..>2364
                     /locus_tag="VYN69_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001808790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1534175.1"
                     /translation="MNSLPNHHFQNKSFYQLSFDGGHLTQYGGLIFFQELFSQLKLKE
                     RISKYLVTNDQRRYCRYSDSDILVQFLFQLLTG"
BASE COUNT          687 a          508 c          395 g          774 t
ORIGIN      
        1 atttaccaat gacaatactc ttaaaacttt catccacaat agtatatggg gaaatttatc
       61 attatttcct acaaagagat acagccaaag aaagcatttt agactatagc tttgctcatg
      121 gatattgtgg aattgcatat gctttatttg cctattctaa agtcttagaa ccttctatgt
      181 tctataatga tctccataca ttccatactg aattaaaaaa attattagaa aaagttactt
      241 ctaatactga aaatttagga aatttacaac tttcttggtg caaaggaatt tccggaataa
      301 tcttatatct ttgtatgtac gattgtgacg gaaacaaaga tattattagt aaatatcaag
      361 aatttgtttt taaccatcat ctaaaaatga tgacaggata ttgccacgga ataactagcc
      421 tactacaaac cactgtctac aatcaaaaca aattactgat gaaaaaaatc caacaggtaa
      481 ttttagcatg ttctgaacga gatgatcacg gtttactgat gtttcaagga gatagtggta
      541 aagcagattt gtttgacttc ggaataggaa gcatggggta tattggtgtc tattaaataa
      601 taaattccca ttcgatgtgc agacataagg agaaaagtat gaaattattt tggacaaaca
      661 acatatatag acagttgctg ctaaacagct gtttttcatc attcggcgac agtattttct
      721 acctcgccat tatcaattat gtggctcaat acaatttcgc tccgctagcg attttactga
      781 tttccatttc agagatggtt cccctactat cgcaactctt tctcgggatt ttaggagatt
      841 ttcaagaaaa tagagtcaaa cacgcactct gtattgccaa aatcaaaatc ctgctctacg
      901 ctattttgac agtatttctc gtcttgtcgc ccttttcatt agtttcagtc attatgattg
      961 tcatcatcaa cctcatctct gacaccttga gctacctgtc tgcctacatg atgaacgccc
     1021 tctacatcag tgtaattaag gacaacctgc atgatgccat ggggttcagg cagtctctga
     1081 tgagggttgt ccgcattgtc gccaatctgg ctggcgtatt ccttatcaat gttatgagta
     1141 ttcaaactat ttcccttatc aacactctga cttttgtcat tgcctttttg ggcctgtatg
     1201 ttattcgaca taccttgtat gaggttgaaa aaaagattga aatgtcacat acagcactga
     1261 gttttaagaa atattttcaa catcttaaac agtcgctggc tgtgctcctg aggttaaaag
     1321 ataccgtcat actactgttt ctgacgacca gtatgattgc catcttggat gtgtcccctc
     1381 ggctgattgc cctccgcttc atccaacaga cactagcaca actgagcatt gggcaactcc
     1441 tcgccctgct ctccatcatc atttcttgtg gagctatcct tggcaatatg accagcagta
     1501 atctatttaa aaatatccgt ttcacgcacc tcttggtttt ctgtgagatt tccctattgg
     1561 ctctaataac tagtatcctt tgtcaaacct atatcgtaat tttcatgacc agtttcatca
     1621 gttctacgat tatcggcatt ctcagccctc gcctacaagc agctgtcttt gcccatatcc
     1681 ccagtgacaa gatgggaacg gtgggctctg ctctgagcac agtagacatt ctcgccccgt
     1741 ccctgctctc cctattagcc ctatccatag catcgggcgt ttcggtgcag ttagcattga
     1801 tatttttgta tcttatttta attgctctta tcttttgtca atggttagtc acgttcaaca
     1861 ctcataacta acgaaaaagc atgtgtagat ttcacatgct tttaatctcc ccaatcgtca
     1921 ggtcaagtac aacaaagtca cttctttgat taagcgagtg ttctaatatc cccaattatt
     1981 caccccaaat ctaaaaacca tccagaatcc ttgccttagc ttagatcctg gatggtttct
     2041 tttttcaccc aatgggtgtt ttttactaga caaaaaagag tttccccttt atggtataag
     2101 tgtagaaaaa aacacaaaaa gaaaggaaac tcacatgaac agtttaccaa atcatcactt
     2161 ccaaaacaag tctttttacc aactatcttt cgatggaggt catttaaccc agtatggtgg
     2221 tcttatcttt tttcaggaac ttttttccca gctgaaacta aaagagcgga tttctaagta
     2281 tttagtaacg aatgaccaac gccgctactg tcgttattcg gattcagata tccttgtcca
     2341 gttcctcttt caactgttaa cagg
//
LOCUS       DAVLVP010000147         2324 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.147, whole genome shotgun sequence.
ACCESSION   DAVLVP010000147 DAVLVP010000000
VERSION     DAVLVP010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.147"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(147..2129)
                     /locus_tag="VYN69_001969"
     CDS             complement(147..2129)
                     /locus_tag="VYN69_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEV1534176.1"
                     /translation="MKSSKLFALAGVTLLAATTLAACSGSGSSTKGEKTFSYIYETDP
                     DNLNYLTTAKAATANITSNVVDGLLENDRYGNFVPSMAEDWSVSKDGLTYTYTIRKDA
                     KWYTSEGEEYAAVKAQDFVTGLKYAADKKSDALYLVQESIKGLDAYVKGEIKDFSQVG
                     IKALDDQTVQYTLNKPESFWNSKTTMGVLAPVNEEFLNSKGDDFAKATDPNSLLYNGP
                     YLLKSIVTKSSVEFAKNPNYWDKDNVHIDKVKLSFWDGQDTSKPAENFKDGNLTAARL
                     YPTSASFAELEKSMKDNIVYTQQDSITYLVGTNIDRQSYKYTSKTSDEQKASTKKALL
                     NKDFRQAIAFGFDRTAYASQLNGQTGASKILRNIFVPPTFVQADGKNFGDMVKEKLVT
                     YGDEWKDVNLADSQDGLYNPEKAKAEFAKAKSALQAEGVTFPIHLDMPVDQTATTKVQ
                     RVQSMKQSLEATLGADNVIIDIQQLQKDEVNNITYFAENAAGEDWDLSDNVGWGPDFA
                     DPSTYLDIIKPSVGESTKTYLGFDSGEDNVAAKKVGLYDYEKLVTEAGDEATDVAKRY
                     DKYAAAQAWLTDSALIIPTTSRTGRPILSKMVPFTIPFALSGNKGTSEPILYKYLELQ
                     DKAVTVDEYQKAQEKWMKEKEESNKKAQEDLAKHVK"
BASE COUNT          655 a          472 c          409 g          788 t
ORIGIN      
        1 agtaaaatta acaaaaattt tctctattct gacaaaaaga ggctccaact tttggggggc
       61 agtacattta gaatctttct ttacaaatgt aaaaaggatt cagcattcaa gagaaatact
      121 aaatcctttc ttatattttg caacagttat ttcacatgtt ttgcgagatc ttcttgagcc
      181 tttttattag actcttcttt ttctttcatc catttttcct gagctttttg gtattcatct
      241 acagtgactg ccttgtcttg aagttccaag tatttatata agattggttc acttgtacct
      301 ttatttcctg acaatgcaaa tggtattgta aatggtacca tcttagacaa gattggacgc
      361 cctgtacgag atgtagttgg aataatcaaa gcactatctg tcaaccaagc ttgggctgca
      421 gcgtatttat catagcgttt agcaacatct gtagcctcat caccagcctc agtaaccaat
      481 ttttcgtagt catatagacc tactttttta gcagctacat tatcttcccc tgagtcaaac
      541 cctaaatatg ttttagtact ttctcctaca gatggtttga tgatatcaag gtaggttgat
      601 ggatcggcaa agtctggacc ccaaccgaca ttatctgata aatcccagtc ttcgccagca
      661 gcattttcag caaaatatgt aatattgttt acttcgtctt tttgtagttg ttggatatca
      721 ataatgacat tatcagctcc taaagttgct tccaaggatt gtttcataga ttggacgcgc
      781 tgaacttttg tagttgctgt ctggtcaact ggcatatcca aatgaattgg gaaagtcaca
      841 ccttctgctt gtaaggctga tttagcttta gcaaattcag ccttggcttt ttctggattg
      901 taaagaccat cctgagaatc tgcaagatta acatccttcc attcatcccc ataagtaacc
      961 aatttctctt tgaccatatc gccaaagttt ttaccatctg cttgaacaaa tgttggtgga
     1021 acaaagatat tacgtaagat tttgcttgct ccagtttgtc cattcaactg agaggcatag
     1081 gctgtacgat caaaaccaaa ggcaatagcc tgacggaaat ccttgtttaa gagagccttt
     1141 ttagtcgatg ccttttgttc gtcgctggtc ttagatgtgt atttatagga ctgacggtca
     1201 atatttgtac caactaaata cgtaatagag tcttgttgag tatagacaat attgtccttc
     1261 atactcttct caagctctgc gaaacttgca cttgttggat agagacgagc tgctgtaagg
     1321 ttaccatctt taaagttttc tgcaggtttg ctggtatctt gaccatccca gaatgacaat
     1381 ttaactttgt caatatgcac attgtcctta tcccagtagt tcggattttt cgcaaattca
     1441 acagaagatt tggtcacaat ggatttcaac aaataaggac cgttatacaa gagactattt
     1501 ggatccgtag ctttggcaaa atcatcccct tttgagttca aaaactcttc attaactggc
     1561 gcaagcacac ccatggttgt ttttgagttc caaaaacttt caggtttgtt caaagtgtac
     1621 tgaactgtct gatcatccag agccttaatt cctacttgtg agaaatcttt gatttcccct
     1681 tttacatagg catccaaccc tttgattgat tcttgaacaa ggtaaagagc atctgatttt
     1741 ttatcagcag catattttag tcctgttaca aagtcttgag ctttgactgc cgcgtattct
     1801 tcaccttcag aagtatacca ttttgcatcc ttacggatag tataagtgta agtcaatcca
     1861 tccttggata cagaccaatc ctcagccata gacggcacaa agttcccgta gcgatcattt
     1921 tctagcaaac catcaaccac gttactggta atatttgctg tcgcagcctt agcagttgtc
     1981 aaatagttga ggttatcagg gtctgtctca taaatgtatg agaatgtctt ctcaccttta
     2041 gtgcttgaac ctgatccaga gcatgcagct aaagtagtcg ccgccaataa tgtcacgccc
     2101 gcaagggcaa atagttttga acttttcatc attttctcct ttaaaacttt ctctccatta
     2161 tagactcttt tcagaaagat gtcaacagaa ttttcagaat ttttgaaaat tatttttcaa
     2221 acaacatctt tgcaaaaaat atgggctcta taatatttgt agtgggtaaa tcccctatgg
     2281 atattatgga gcctattttt gtgtagaaaa aaagtcccat aaga
//
LOCUS       DAVLVP010000148         2317 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.148, whole genome shotgun sequence.
ACCESSION   DAVLVP010000148 DAVLVP010000000
VERSION     DAVLVP010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.148"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..415)
                     /locus_tag="VYN69_001970"
     CDS             complement(<1..415)
                     /locus_tag="VYN69_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000406168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEV1534177.1"
                     /translation="MEISKGIIFNIQHFSIHDGPGIRTTVFLKGCPLRCPWCSNPESQ
                     RMKPEKMKDAQREKFTLVGEEKTVEEIITEVLKDKEFYEESGGGLTLSGGEIFAQFEF
                     AKAILKSAKEHHIHTAIETTAFVDHEKFIDLIQYVD"
     gene            537..1283
                     /locus_tag="VYN69_001971"
     CDS             537..1283
                     /locus_tag="VYN69_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEV1534178.1"
                     /translation="MKRLEQIIKLVSEYEKIDVNTLSEKLNVSKVTIRKDLDKLESKG
                     LLHREHGYAVLNSGDDLNVRLSINYEIKRKIVQEAVKLVSDNETIMIESGSTCALLAE
                     EICKQKRNVTIVTNSFFIANFVRAYDSCRIIVLGGEFQKDSQVTVGPLLKEMIQTFHV
                     HQAFVGTDGYDKEMGFTGKDLMRSEVVQYISAASDKVIVLTDSSKFDKRGTVRRFALS
                     QVYEVITDEKLSKQNIATLENAGIMVKVVS"
     gene            1296..2276
                     /locus_tag="VYN69_001972"
     CDS             1296..2276
                     /locus_tag="VYN69_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HEV1534179.1"
                     /translation="MNQDRNKLLSKIAYLYYIENLNQSQIAAKLGIYRTSISRMLTEA
                     RNAGIVKIEIENFDTNMFKLENYVKEKYGLESLEIIPNEFDDNPTILSERISQVAAGV
                     LRNLIDDNMKIGFSWGKSLSNLVDLIHSKSVRNVHFYPLAGGPSHIHAKYHVNTLIYE
                     MSRKFHGECTFMNATIVQENKLLADGILQSRYFENLKNSWKDLDIAVVGIGDFSNKGK
                     HQWLDMLTEDDFKELTKVKTVGEICCRFFDSKGKEVYENLQERTIAISLEDLKNIPQS
                     LAVAYGDTKVSSILSVLRANLVNHLITDKNTILKVLEEDGDLTFREILGE"
BASE COUNT          836 a          284 c          440 g          757 t
ORIGIN      
        1 aatccacata ttgaattaaa tcaataaatt tttcatgatc aacaaaggca gtagtttcaa
       61 tggcagtgtg tatgtgatgt tctttagctg attttaagat ggctttagca aattcaaact
      121 gagcaaatat ttcacctcct gataaagtta aacctccacc ggattcttcg taaaattctt
      181 tgtcttttaa tacctctgta ataatttctt ctacagtctt ttcttcaccg actaaggtga
      241 atttctctcg ttgagcatct ttcatttttt caggtttcat tctttgagat tcaggattag
      301 aacaccatgg acagcgcaga ggacatcctt ttaaaaaaac agttgtacga atacccgggc
      361 cgtcatgaat tgaaaagtgt tgaatattaa aaataattcc tttagatatt tccataagag
      421 ttcctcctct tcataagtct atcctatcac aaaaacgaaa gaaaaacaat tacgaacgaa
      481 agtttaattt gttgtttttt gctttttatt ttggtagaat aaaacggagg tgtaaaatga
      541 aaagattgga acaaattatt aaattagtat cagaatatga aaagatcgac gttaatacat
      601 tatcggaaaa attaaatgta tcgaaagtaa cgattagaaa agatttagat aaattagagt
      661 caaaaggttt attacacaga gagcatggat atgctgtatt aaatagtgga gatgacttaa
      721 atgtacgttt gtcaattaac tatgaaatta agagaaaaat tgttcaggaa gcagtaaaat
      781 tggtgtcaga taatgaaaca ataatgatag aatctggatc gacctgtgct ttacttgctg
      841 aggaaatttg caagcaaaaa agaaatgtta cgattgtaac aaattcattt tttatagcaa
      901 attttgtgag agcttatgat tcatgtcgta ttattgttct tggtggagag tttcagaagg
      961 attcacaggt tactgtggga cctttattaa aagaaatgat acagactttt catgtgcatc
     1021 aagcttttgt tgggacagat ggttacgata aagagatggg ctttacagga aaagatttaa
     1081 tgcgcagtga ggtagttcaa tatatttcag cagcgtcgga taaagtcatt gtactaactg
     1141 actcaagtaa atttgataaa aggggtacag taagaagatt tgctttaagt caagtctatg
     1201 aagtaataac agacgaaaaa ctttctaaac aaaatatagc tacattagaa aatgctggga
     1261 taatggttaa ggtagtttcg taagaggtta aatgtatgaa tcaagatagg aataaactgc
     1321 tctctaaaat tgcttatctg tattatattg aaaacttaaa tcagtcacaa atagcagcaa
     1381 aattaggaat ttatagaacc tctattagta gaatgttaac agaagcaagg aatgcaggaa
     1441 ttgttaaaat tgaaatagag aattttgata ccaatatgtt taagttggaa aattatgtaa
     1501 aagaaaaata tggtctggaa agtttagaaa ttattccaaa tgaatttgat gataatccaa
     1561 caattttatc tgaaagaatt tctcaagttg cagcaggtgt ccttaggaat ctaattgatg
     1621 ataatatgaa aattggcttt tcttggggga aaagtttaag taatttagta gatttaattc
     1681 acagtaaaag tgtccgaaat gttcacttct atcctctagc aggtggtcct agtcacatac
     1741 acgctaaata ccatgtgaat acactgattt atgaaatgtc tagaaaattt catggagagt
     1801 gtacatttat gaatgcaacg attgtgcaag aaaataaatt gttagcagat ggtattttgc
     1861 aatcaagata ttttgaaaat ttgaaaaata gttggaaaga tttagatata gctgtagtcg
     1921 gaattggtga ttttagcaat aaaggaaagc atcaatggtt agacatgctt acagaggatg
     1981 actttaaaga attgaccaaa gttaaaactg taggagaaat ttgttgtcga ttttttgatt
     2041 caaaaggtaa agaagtctat gaaaacttac aggaaagaac gatagcaatc tctttggaag
     2101 atttaaaaaa tattcctcaa agtttagctg ttgcttacgg tgatacgaaa gtatcttcga
     2161 ttctttctgt cttgcgtgct aatttagtaa atcatttgat tacagacaaa aatacaattt
     2221 taaaagtttt ggaagaagat ggggatttga cttttagaga gattctaggt gagtgaaaat
     2281 gatagactga ttcagtttat cgtttttctt tttagtt
//
LOCUS       DAVLVP010000149         2249 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.149, whole genome shotgun sequence.
ACCESSION   DAVLVP010000149 DAVLVP010000000
VERSION     DAVLVP010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.149"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            95..1618
                     /locus_tag="VYN69_001973"
     CDS             95..1618
                     /locus_tag="VYN69_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016648332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1534180.1"
                     /translation="MFSHILGFRLETNLRKRGIDGLEKASFRFFDLNPSGQIRKIIDD
                     NAAQTHQVVAHMIPDSSQAIITPVLVLALGFIVSIRVGIILLALTIIGGLILGAMMGE
                     QEFMKIYQESLSKLSAETVEYVRGMQVVKIFKANVESFKSFYKAIKDYSKYAYDYSLS
                     CKRPYVLYQWLFFGLIAILIIPIVYFMTSLASAKVILLELIMILFLSGVLFVSFMRMM
                     WYSMYISQGNYAVDTLEALYEDMQKDKLVHGNVNNFKNYNIEFENVSFAYNDKAVIEN
                     LSFNLEEGKSYALVGSSGSGKSTVAKLISGFYNVNKGSIKIGGIAISEYSDEALIKAI
                     SFIFQDSKLFKKSIYDNVALANKDATKDDVMRALKLAGCDLILDKFPERENTIIGSKG
                     VYLSGGEKQRIAIARAILKDSKIIIMDEASASIDPDNEFELQKAFKNLMKDKTVIMIA
                     HRLSTIKDLDEIIVMDSGKIIERGSDKELMSKDTRYKSLQEMFNSANEWRVSNERVL"
     gene            1584..>2249
                     /locus_tag="VYN69_001974"
     CDS             1584..>2249
                     /locus_tag="VYN69_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001847123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1534181.1"
                     /translation="MNGGFQMKEFYKKRFALTDGGARNLSKATLASFFVYCINMLPAI
                     LLMIFAQEVLENMGKSNGFYIVFSVLTLIAMYILLSIEYDKLYNTTYQESADLRIRTA
                     ENLSKLPLSYFSKHDISDISQTIMADIEGIEHAMSHSIPKVGGMVLFFPLISVMMLAG
                     NVKMGLAVIIPSILSFIFIPLSKKYQVNGQNRYYDVLRKNSESFQENIEMQMEIKAYN
                     LSKD"
BASE COUNT          799 a          308 c          420 g          722 t
ORIGIN      
        1 aataattaat tcaaacttat ccggtgcaga gagtatagca ttaaaatctg ttattacact
       61 aacaagtgga gcgatatttt attttgtctc aggaatgttt tcacatatct tgggattcag
      121 gcttgaaaca aatttaagaa aaaggggaat cgatggtctg gaaaaagcaa gttttaggtt
      181 ctttgactta aatccatctg gtcaaataag aaagattata gatgacaatg ctgcacaaac
      241 tcatcaggtg gtagcacaca tgattcccga tagttctcag gcaataatca cacccgtact
      301 tgtacttgca cttggcttta tagtaagtat aagagttggc ataattttgc ttgctcttac
      361 tataattggt ggcttaattt taggggcaat gatgggcgag caagaattta tgaagatata
      421 ccaagaatcc ctatctaaac taagtgctga aactgttgag tacgtgagag gaatgcaagt
      481 tgtaaaaata tttaaagcaa atgtagagtc ttttaaaagc ttttataagg cgataaaaga
      541 ttactcaaag tatgcttatg attattccct atcttgtaaa aggccttatg ttttgtatca
      601 atggttattt tttggactga ttgcaatttt aattattcct atagtttatt ttatgactag
      661 cttagctagc gcaaaggtga ttttacttga gcttatcatg attttatttt tatcaggagt
      721 tctctttgtt tcattcatga gaatgatgtg gtactccatg tatatttctc aaggaaatta
      781 tgcagtagat actttagagg cgctttacga agatatgcaa aaagacaaat tagtgcatgg
      841 taatgtcaat aattttaaaa actataatat agaatttgag aatgttagct ttgcttataa
      901 tgataaagct gtcattgaaa atttatcctt taatttagaa gaaggaaagt cctacgcact
      961 tgtcggttca tctggatcag gcaaatcaac agtagcaaaa cttatatcag gtttttacaa
     1021 tgttaataaa ggaagcataa agataggcgg gatagcaata agtgaatatt ctgacgaagc
     1081 cttaattaaa gccatttcct ttatttttca agattcaaaa ttattcaaga agagcattta
     1141 tgataatgta gctttagcta ataaagatgc gacgaaagat gacgttatga gagccttaaa
     1201 attagcagga tgcgatttaa tattagacaa attcccagaa agagaaaata caatcatagg
     1261 ctcaaaaggt gtttatttat ccggtggaga aaaacaaaga attgcaattg ctagagcaat
     1321 tttaaaggat tccaaaatta ttattatgga tgaagcatca gcatctattg acccagataa
     1381 cgagtttgaa ttgcaaaaag cttttaaaaa tcttatgaag gataaaacag ttatcatgat
     1441 tgcacacagg ctatctacaa ttaaagacct tgatgaaatt attgtcatgg atagtggaaa
     1501 aattatagaa agagggtctg acaaagaatt aatgtcaaaa gatacaaggt ataagagcct
     1561 gcaagagatg tttaacagtg cgaatgaatg gagggtttca aatgaaagag ttttataaaa
     1621 aaagatttgc tcttacagat ggaggagcaa gaaatttaag taaagcaaca ctggcttcat
     1681 ttttcgttta ttgtataaac atgcttcctg ccatattact tatgattttt gctcaggaag
     1741 ttttggaaaa tatgggcaaa agcaatggct tttatatagt attctcagtt ttgactttga
     1801 tagcaatgta tattttgctt tctatcgaat acgataaatt atataacaca acctatcaag
     1861 aaagtgcaga tttaagaata aggacagcgg agaatttatc aaaattacct ctatcttact
     1921 tttctaaaca tgacatttcc gacatttcac aaacaatcat ggctgatatt gaaggcatag
     1981 agcatgcaat gagccactca ataccaaagg tgggcggcat ggtactgttt ttcccattaa
     2041 tatctgtaat gatgctagcg ggcaatgtca agatgggttt agctgtaatt attccatcta
     2101 ttttaagctt tatatttata cctttatcta aaaaatatca ggttaatgga cagaatagat
     2161 attatgatgt cttaagaaaa aactcagaaa gctttcaaga aaatatcgaa atgcaaatgg
     2221 agattaaagc atataattta tcgaaggat
//
LOCUS       DAVLVP010000150         2202 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.150, whole genome shotgun sequence.
ACCESSION   DAVLVP010000150 DAVLVP010000000
VERSION     DAVLVP010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2202
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.150"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            43..1266
                     /locus_tag="VYN69_001975"
     CDS             43..1266
                     /locus_tag="VYN69_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lanthionine synthetase C family protein"
                     /protein_id="HEV1534182.1"
                     /translation="MLDNNIIDLLQRSCYTENRSYLSEYPSIIIYLSYRLANCDDNEH
                     SKLLYNRVNYYLHELLKSIKLNSRNNISMCYGFSGYVYALKLLPKRSKEYSKLLETLE
                     TILVSLTRDRLSEIKKSNKVKEEYIDVIQGVSSVGKYFLSKDKLTSNQELLLKGVLNY
                     LAGVINNKPTIYPEYMPNEKLKRKFPNGYINLGVAHGILGPLYVLALGFKKFNMPEYL
                     ISLKKGLSYYEKTFQTNKIGKIIGWNGRVSAEVESEKFEYNLSWCYGSLGMARVLYNI
                     SKIIDIPKLQELATDVFHSSIYYLNSSEILNNAICHGRSGIMLLFNLMYLDTGESQFK
                     AISDNLFKEIVNKATDSEYIFVERDIYFRGVNYDEVIEYIDFGLLNGVSGIVLALMAQ
                     RTGNASPLAEMFFMQ"
     gene            1295..>2202
                     /locus_tag="VYN69_001976"
     CDS             1295..>2202
                     /locus_tag="VYN69_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000722643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEV1534183.1"
                     /translation="MKKILNNKLYMKVLVSDLISNFGDTLYFIALMTYVTEIKSSNLA
                     ISIVNISETIPILFTIFFGIIADRTLNKVGMIIKTLWIRTILYLLVAVVMNFKESILV
                     VILASIVNLISDTLGQFENGLFYPISNRIVKKSDREETMAFRQTATSTMNIVNQSLGA
                     FLITFLSFFHLALINSLTFAISLLITLAIKSQINNFYIDKTPSTKVSKVDFKATFSDI
                     ISNLKLSLKHLFSLTNMKTVLLVIPILNGSLAIIIPLAVVNLSKSSALTIISSATTIS
                     VLGISTVSGGILGGTLILISKKFKNLS"
BASE COUNT          766 a          286 c          390 g          760 t
ORIGIN      
        1 attaagaagt atgatgaaat catcaataaa gtagatagtc tagtgttaga caataatatt
       61 attgatttac tacagcgctc gtgctatacg gaaaatagaa gttacttgtc ggaatacccc
      121 agcataatca tatatttatc atatagatta gctaattgcg atgataacga gcattctaag
      181 ttgctctaca atagagtaaa ctactattta catgaactgt taaagtcgat taagttgaac
      241 agtcgtaata atatatcaat gtgctatgga ttttctggct atgtatatgc cttgaaattg
      301 ttacctaaga ggagtaaaga atatagtaaa ttgctagaga cacttgaaac aattttagta
      361 tctttaacta gagataggct aagtgaaata aaaaaatcca ataaagttaa agaagaatat
      421 attgatgtaa tccaaggagt ttctagtgta ggaaagtatt ttcttagcaa ggataaattg
      481 acttctaatc aggaactatt attaaaagga gttttaaatt atttagcagg agtaataaat
      541 aataagccaa ctatttatcc ggaatatatg ccaaatgaaa aattaaagag aaaattcccc
      601 aatggctata tcaacctagg tgtagctcat ggtatattag gaccactata tgttttagca
      661 ttagggttta aaaaattcaa catgccagag tatttaattt cgctaaaaaa gggtttgagt
      721 tactatgaaa aaacttttca aactaataag attggcaaga ttattggctg gaatggtaga
      781 gtaagtgctg aggtagaaag tgagaaattc gaatataatt taagttggtg ttatggtagt
      841 cttggaatgg caagagtact atataatata tctaaaataa ttgacatccc aaaattacaa
      901 gagttagcta ctgatgtatt ccactcatct atttattacc ttaatagtag tgaaatttta
      961 aataacgcaa tttgtcatgg tcgctctggg ataatgcttt tgttcaactt gatgtattta
     1021 gacacagggg aatcacagtt taaagctatt tctgataatt tatttaagga aatagtaaat
     1081 aaagccactg attctgaata catatttgtg gaacgtgata tatattttag gggtgtgaat
     1141 tatgatgaag tgatagagta tattgatttt ggcttgttaa atggggtttc tgggatagtt
     1201 cttgcactaa tggcgcagag gacaggaaat gcttctcctt tggctgaaat gttttttatg
     1261 cagtgagggt ttaaggtaga tatagggata acatatgaaa aaaattttaa ataataagct
     1321 atatatgaaa gtcttggttt cagatctgat atcaaacttt ggagatacac tatattttat
     1381 agctctcatg acttatgtga cggagattaa gagtagcaat ttggctatct caatcgtaaa
     1441 tatatcggag actataccta tattgttcac aatatttttt ggtatcattg cagataggac
     1501 acttaataaa gtgggaatga ttatcaaaac gttatggata cggacaattt tatatttatt
     1561 agtggctgtt gtcatgaatt ttaaggaatc cattttagta gtaatacttg ctagtatagt
     1621 taatcttatt tcagatacat tggggcaatt tgaaaatggt cttttctatc caatttctaa
     1681 tcgaatagtg aaaaaatcag atagggaaga aaccatggcg tttaggcaaa cagcaacttc
     1741 taccatgaac attgtaaatc agtcgttagg ggcattttta atcacttttc ttagtttctt
     1801 tcacttagct cttataaatt ctctaacatt cgctattagt ttactaataa cattagctat
     1861 caaaagccaa attaacaatt tttatataga caagacccca tccactaagg tttcaaaagt
     1921 tgattttaaa gctacgtttt ctgatattat ttcaaactta aaactttcgc ttaaacatct
     1981 atttagtctc actaatatga agactgtatt acttgttatt ccaattctta atgggagctt
     2041 agccattatc ataccactag ctgtagtgaa tctttctaaa agttctgctc ttacaattat
     2101 tagttctgca acaacaattt cagttttggg aattagtact gtttctggag ggattttagg
     2161 tggaactttg attctaatta gtaaaaaatt taaaaatctt tc
//
LOCUS       DAVLVP010000151         2166 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.151, whole genome shotgun sequence.
ACCESSION   DAVLVP010000151 DAVLVP010000000
VERSION     DAVLVP010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.151"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..790
                     /locus_tag="VYN69_001977"
     CDS             <1..790
                     /locus_tag="VYN69_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEV1534184.1"
                     /translation="DTDIAYRKLKKKFRHISLTGDELKYKMEFSNKVWICWFQGEEHA
                     PELIKTCIQSMRNQLQGREIIVLTEKNISDYTEIPDYIIEKYKKGWISRAHYSDILRI
                     DLLCRHGGLWVDATVLNTGGDFSNLEVPLFVYKSLNLSRKDSQAIVASSWLISSYSNH
                     PILLYTRKLLWAYWRRKNSLCNYFLFHICFTIATERYPIEWSAVPTFNNHSPHILHFE
                     LNEQFSEKRWEQLKHISAFHKLNHHIDYSSGVNTFYKFIVSSKV"
     gene            795..>2166
                     /locus_tag="VYN69_001978"
     CDS             795..>2166
                     /locus_tag="VYN69_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HEV1534185.1"
                     /translation="MSNKISKNLAYNMGYQLIGIAAPLITSPYLSRILGAENLGIHSF
                     TMSVALYFMMFMLLGIANYGNRTIATVKREGKDILSKTFWSIYSIQLIMSILVTIAYL
                     AYLYLGAVHYKVIAILQLFLLLSNAVDITWFFYGLENFKQIVFRNTLVKLLGLFLIFL
                     CVRQGTDLWKYTFINGSVTLVGQLLLWGQLKGILTWKKIQLKELLPHIKPILILFIPV
                     LAISIFTNMDKYMLGLMVDVKQVGFYDNAGRIIEIPKALIAALGAVMLPRTSYLLAEG
                     QKEKSKYYIEVTILYVMIISSVLMFGLISVSDIFSIIFWGEEFLESGRLISAMSPAFV
                     FSVLGNIIRTQYLIPRAKDKDYVVSLIAGAVVNLVLNYFFIKPFGAMGATISTVLAEF
                     VLSGMQFWSVRRDLDLKRYLKNGLIFYLFGLIMHLIIIALKTQLPYNIMSLILLIVLG
                     GIVYVIF"
BASE COUNT          700 a          287 c          396 g          783 t
ORIGIN      
        1 agataccgat atagcatata gaaaactaaa gaagaagttt agacatattt cgttgactgg
       61 agatgagctt aaatataaaa tggagttttc aaataaagtt tggatttgtt ggtttcaggg
      121 agaagagcat gccccagagt taattaaaac atgtattcaa tcaatgagga atcaacttca
      181 aggaagagaa atcattgtgt tgacagaaaa aaatataagt gattatacag agattccaga
      241 ctatattatt gaaaaataca aaaaaggatg gatttctcgt gcgcattact ctgatatcct
      301 taggatagat cttctatgtc gccatggagg attatgggta gatgcaacag tgttgaatac
      361 aggaggagat ttctcaaatc ttgaggtacc actttttgta tataaatctt tgaatttatc
      421 aagaaaagat tcacaagcta ttgtagcatc aagttggtta atttcgtcat atagtaatca
      481 tcctatatta ctctatacga gaaaactcct atgggcctac tggcgaagaa aaaatagttt
      541 atgtaattat tttttgttcc atatatgttt tacaatagct acagagcggt acccaatcga
      601 atggtctgct gtccctactt ttaataatca ttctcctcat atacttcatt ttgagttgaa
      661 tgaacaattt tcggaaaaac ggtgggaaca actgaagcat atttcggcgt ttcataaatt
      721 aaatcatcat atagattatt ctagcggtgt taacactttt tataagttca tagtatcttc
      781 gaaagtttag aaaaatgagt aataaaatta gtaaaaattt agcctacaat atggggtatc
      841 agttaattgg tattgcagct cccttgataa cttctcctta tttatcacgg atattaggtg
      901 ctgaaaattt aggtattcat tcttttacta tgtcggttgc actttacttt atgatgttta
      961 tgcttcttgg aattgcaaat tacggaaata gaacaattgc gactgttaaa cgagaaggaa
     1021 aggatattct atcaaaaacg ttttggagta tctactctat ccagttgata atgtctattc
     1081 tggtaactat agcttatctt gcttatctat atttgggggc agtacattac aaagttattg
     1141 caatattaca actcttttta ttgttatcaa atgctgttga tattacttgg tttttctatg
     1201 gattagaaaa ttttaagcaa atcgttttta ggaatacatt agtcaaactt ttaggattat
     1261 ttttaatttt tttatgtgtg cgtcaaggca cagatttatg gaaatacaca tttataaacg
     1321 gatcagtaac tttagttggc caacttttac tatggggaca attaaaagga atattaactt
     1381 ggaaaaaaat tcaattaaag gaattactac ctcatatcaa gccaatttta attcttttta
     1441 ttcctgtact agccattagt atttttacta atatggataa atatatgctt ggtttaatgg
     1501 ttgatgtaaa gcaggttgga ttttatgaca atgcaggtag aattattgaa attcctaaag
     1561 ctttgattgc agctttaggg gccgttatgt taccaagaac ttcctattta ttagcggaag
     1621 gacagaaaga aaagagtaaa tactacatag aggtaacaat tttatacgtg atgattattt
     1681 ctagtgtatt gatgtttggg ctaatatcgg tatctgatat tttttccatt atattttggg
     1741 gagaagaatt tcttgaatca ggtagattga tttctgctat gtctccggct tttgtcttct
     1801 ctgtattagg aaatatcatt aggactcagt atttgattcc tagagctaag gataaagatt
     1861 atgtagtgtc tctaattgcg ggggctgttg ttaatctagt gctaaactat ttttttataa
     1921 aaccttttgg tgctatggga gcaacaatta gtacagtttt ggcagaattt gtattgtctg
     1981 gaatgcagtt ttggtcagta agaagagact tagatttaaa aagatatttg aaaaacggtt
     2041 tgatattcta cctatttggt ctgattatgc acctcattat tatagcactc aaaactcagt
     2101 taccatacaa tataatgagt cttattttac taatagtttt gggtggaata gtatatgtta
     2161 tttttt
//
LOCUS       DAVLVP010000152         2134 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.152, whole genome shotgun sequence.
ACCESSION   DAVLVP010000152 DAVLVP010000000
VERSION     DAVLVP010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.152"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(142..543)
                     /locus_tag="VYN69_001979"
     CDS             complement(142..543)
                     /locus_tag="VYN69_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEV1534186.1"
                     /translation="MLFFSISNDLLQLLISWEKQQRYELAKFGILSNPEQFVFTYIDT
                     KGNINKPLHADYLNNKMKSIRKRHKELTHATPHKLRHTGATLAKKAGMSLEAISKALT
                     HSDTGTTQIYVNTSNVVPMTVGEFALKSLKQ"
     gene            complement(919..2127)
                     /locus_tag="VYN69_001980"
     CDS             complement(919..2127)
                     /locus_tag="VYN69_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001865288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEV1534187.1"
                     /translation="MNYIKRPHYLDFLRRHRDRQIIKVVSGVRRAGKSVLFQLYKEEL
                     LATGVDEDQIISINFEDLSYYDLRHFQTLFAYIKDQLVSKKTYYIFLDEIQHVEKFEL
                     VADSLFILANVDLYLTGSNAYFMSSQLTTNLTGRYVEIEVLPLSFEEYLSGQSLTENL
                     NTTEIFNNYLFSAFPYLLQTSSYAEKIDYLRGIYNSILLNDIVTRLGNPNPTIIERIV
                     RTLLSSTGSLMSTNKIRNTLVSQNVSISHNTLENYLTTLTDSLLFYSVPRFDVKGRAL
                     LQRLEKYYPVDLGLRHLLLPDHKEDIGHILENMVYLELRRRYSQVYVGNLDKYEVDFV
                     VVTDLGHYAYYQVSETTLAPETLERELRPLEAIKDQFPKYLLTMDTIQPTANYNGIEK
                     KNIIDWLLEK"
BASE COUNT          666 a          339 c          363 g          766 t
ORIGIN      
        1 gtttcaaagc actcaaaagt tcaccttatt tttatagtaa aatgaaataa gaataggaca
       61 aatcgatcag gacagtcaaa tcgaattcta acaatgtttt agaagtagag gtgtactatt
      121 ctagtttcaa tctactatac cttattgttt tagagacttt aaagcaaatt cacctactgt
      181 cataggaact acattagaag tatttacata aatctgtgtt gttcctgtgt cactgtgtgt
      241 tagagcctta gaaatggctt ctaagctcat tcctgctttt ttagcaagtg tcgctcctgt
      301 atggcgtaat ttgtgaggtg tagcgtgtgt cagttcttta tgtcgctttc taatgctttt
      361 cattttattg ttcagatagt cggcatgtaa aggcttgttg atgtttcctt ttgtgtcgat
      421 atatgtaaaa acaaattgtt ctggattact gaggatgcca aactttgcta gttcatatct
      481 ttgttgtttt tcccaagaga taagaagttg aagtaaatca ttagaaatag agaaaaatag
      541 gatagcaaaa gctctatttc aggagaaaat gaagcaaata tttccataat aaaacgcata
      601 ttatcaaggt tgttcaatac ctgatactat gcgcttttct gattttaaag actgattgct
      661 cagtctcttt ttcattataa ttcagttaca atttaatctt atttgagaaa gtgggtgatg
      721 ctattctcta ataagactat gatattaatt tttgatagta cttccagaaa ttatagatcc
      781 cctttcatcc ccaatttcta ttttcaagtg tccgtattat atagcgtatt cgaaattcac
      841 tatatcttgt taggatttgg ggagaatcat tggtaacaga ctgttgcaaa tctaattagc
      901 tgtatgattt atatttatct atttttctag taaccaatct ataatgtttt tcttctcgat
      961 tccattgtaa ttggctgttg gctgaatcgt atccattgtt aatagatatt tagggaattg
     1021 atctttaatg gcttctagtg gtctaagttc tctttctagt gtttctggag caagtgttgt
     1081 ttcactgacc tgataataag cgtagtggcc aagatcagtt acaacaacaa aatcaacctc
     1141 atacttatct aaattaccaa catatacttg tgaatatcta cgtctcaatt ccaaatatac
     1201 catattttcc aagatatgcc caatgtcttc tttgtggtct ggtaataaga gatgtcgtaa
     1261 acctaaatca acgggataat atttttctaa gcgttgcaat aatgctctac cttttacatc
     1321 aaaacgtgga acggaataaa aaagtaaact atctgtcaaa gttgtcaaat aattttccaa
     1381 agtattatgg gatattgaaa cattttggct gactagggta ttgcgaatct tatttgttga
     1441 cattaagcta cctgtactac tgagaagggt tcggacaatg cgttcaataa tagtaggatt
     1501 tggatttccc aatctagtga caatatcatt taacagtatg gagttatata ttcctctgag
     1561 atagtcaatt ttttcagcgt aagatgatgt ttgcaataag taagggaaag cactaaagag
     1621 ataattgtta aaaatttctg ttgtattcag attctctgtg agagattgac ctgatagata
     1681 ttcttcaaat gacaaaggaa gaacctctat ctcaacatac cgaccagtca agtttgttgt
     1741 taattggcta ctcataaagt aggcgttaga tccagtcaaa tagaggtcta catttgctaa
     1801 gatgaataga ctatctgcta ccagttcaaa tttttcaaca tgttgaattt catctaaaaa
     1861 gatatagtat gttttcttgc taactaattg atcttttata taagcgaata atgtttgaaa
     1921 atgtcgcaaa tcatagtaac tcaaatcttc gaaattgatg gatataatct gatcctcgtc
     1981 taccccagtt gctagtaact cctctttata gagttgaaaa agcacagatt taccagctcg
     2041 tctaactcca ctcacaactt tgatgatttg tcggtcacga tgtcttctta aaaaatctaa
     2101 ataatgtggt cttttgatat agttcataat agtt
//
LOCUS       DAVLVP010000153         1942 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.153, whole genome shotgun sequence.
ACCESSION   DAVLVP010000153 DAVLVP010000000
VERSION     DAVLVP010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.153"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            253..1194
                     /locus_tag="VYN69_001981"
     CDS             253..1194
                     /locus_tag="VYN69_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEV1534188.1"
                     /translation="MLTYFVTLKIIYYFHLELAYFDEDYLYPFLKKVFFALLLLHIVS
                     FVSDMVSTIKHLNSFGSILSSIVFISLLLTFFAMNSHKVQMEKEIALKQKKFEQKHLQ
                     NYTDEIVGLYNEIRGFRHDYAGMLVSMQMAIDSGNLQEIDRVYNEVLVKANHKLRSDK
                     YTYFDLNNIEDSALRSLVAQSIVYARNNGVEFTLEVKDTITKLPIELLDLVRIMSVLL
                     NNAVEGSADSYKKQMEVAVIKMETETVIVIQNSCKMTMTPSGDLFALGFSTKGRNRGV
                     GLNNVKELLDKYNNIILETEMEGSTFRQIIRFKRKFE"
     gene            1191..>1942
                     /locus_tag="VYN69_001982"
     CDS             1191..>1942
                     /locus_tag="VYN69_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000866085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEV1534189.1"
                     /translation="MKVLILEDVIEHQVRLERILDEISKESNTPISYKTTGKVREFEE
                     YIENDEVNQLYFLDIDIHGIEKKGFEVAQLIRHYNPYAIIVFITSRSEFATLTYKYQI
                     SALDFVDKDINDEMFKKRIEQNIFYTKSMLLENEDVVDYFDYNYKGNDLKIPYHDILY
                     IETTGVSHKLRIIGKNFAKEFYGTMTDIQEKDKHAQRFYSPHKSFLVNIGNIREIDRK
                     NLEIVFYEDHRCPISRLKIRKLKDILEKKSQK"
BASE COUNT          657 a          277 c          358 g          650 t
ORIGIN      
        1 atagtacttg attttccctt ctatcttcta tatttagatg ggttagggat tgcaacattt
       61 ttatttcctt tgggcctata ttcctatttt cgatggatga aacagtatga gagggataga
      121 ggactatttc taagtttact actatctctt ttatatgaga gcactcataa ctttctgtcc
      181 gtaactttct cttctataac aggagacaat tttgttttac agtattatgg cctattcttt
      241 ttcgttgtga cggtgttgac ctattttgtt acattaaaaa tcatttatta tttccatttg
      301 gaactagcct attttgacga ggactacctt tatcctttct tgaaaaaagt attttttgct
      361 ttactattgc tacatattgt atctttcgtt tcagatatgg taagtacgat taaacatttg
      421 aatagttttg gaagtatttt gtcatctatt gtctttatct ctctcctttt gaccttcttt
      481 gcaatgaatt ctcataaagt tcaaatggag aaagagattg ctttgaagca gaagaaattt
      541 gaacagaaac atttacagaa ttacacagat gaaattgtcg gtctgtataa tgaaatccgt
      601 ggttttcgac atgattatgc tggaatgctt gtcagcatgc agatggcaat tgacagtggt
      661 aatttacagg aaattgacag agtttacaat gaagttttgg tcaaagcaaa tcataaacta
      721 cgttcagata agtacactta ctttgatttg aacaacatag aagattcagc tttacgaagt
      781 ttggttgctc agtcaattgt ctatgctcga aataatggtg tagagtttac actggaagta
      841 aaagatacga ttaccaagct tccaattgaa ctattggatt tagtgcgtat catgagcgtt
      901 ttattgaaca atgctgtcga aggatcggct gatagctata aaaagcagat ggaagtagca
      961 gttattaaga tggaaactga aacagttatt gtgattcaga attcatgtaa aatgacgatg
     1021 actccttcag gagatctatt tgccttagga ttctccacta agggaagaaa tcgcggagtc
     1081 ggattaaata atgtgaaaga actactagat aagtacaaca acattatttt agaaacagag
     1141 atggaaggca gtacatttag acaaatcatt agatttaaga ggaaatttga atgaaagttt
     1201 taattttaga agatgttatt gaacatcaag tgagactaga gagaatattg gatgaaattt
     1261 cgaaagaatc gaatactcca atatcataca agacaacggg aaaagtccgt gaatttgaag
     1321 aatacattga aaatgatgaa gtaaatcaac tttatttcct agatatcgat attcatggaa
     1381 ttgagaaaaa gggatttgaa gttgctcaac tcattcgtca ttacaatcct tacgctatta
     1441 tcgtctttat caccagtcga tcagagtttg cgactctgac ctataaatac cagatatcag
     1501 ccctagattt tgttgataag gatatcaacg atgagatgtt taagaagcga attgagcaaa
     1561 atatcttcta cacgaagagt atgttacttg aaaatgaaga tgttgtagat tatttcgact
     1621 acaattacaa gggaaatgat ttaaaaattc cttaccatga tattttgtat attgaaacga
     1681 caggagtctc tcataaattg cgcattattg gtaagaattt tgcaaaagag ttttatggta
     1741 ccatgacaga tattcaggaa aaggacaaac atgctcagcg attttattct cctcacaagt
     1801 catttttggt aaatataggc aatatcagag aaattgatcg aaaaaactta gaaattgttt
     1861 tctatgaaga ccatcgttgt cctatttcaa gattaaaaat tagaaaatta aaagatattt
     1921 tagagaaaaa atctcaaaag tg
//
LOCUS       DAVLVP010000154         1838 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.154, whole genome shotgun sequence.
ACCESSION   DAVLVP010000154 DAVLVP010000000
VERSION     DAVLVP010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1838
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.154"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..184
                     /locus_tag="VYN69_001983"
     CDS             <1..184
                     /locus_tag="VYN69_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001017606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HEV1534190.1"
                     /translation="VGIKILVYEPNLNVSIDNVIFEKNFELFTKQSDLIVANRWDRGL
                     EAYKDKVYTRGIWIRD"
     gene            579..1442
                     /locus_tag="VYN69_001984"
     CDS             579..1442
                     /locus_tag="VYN69_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE/MutR family transcriptional regulator"
                     /protein_id="HEV1534191.1"
                     /translation="MIEKMELGEFYKELRLARKLKQSDVACAGLTASQLSKFELGQSM
                     LSADKLILAIQGINVTFDEFGHKLNNYQESPHMQIGRKVVDRFAHQDIAGLEQLLEEV
                     EQGQMAETYRRLNAIVIKNALHSLDKSYPLAEEDSEFLTTYLYAIESWTWFELYLFCN
                     TMPFLSNQDLIFLSTSLLEKSKEFKELVHNRLYMKSGFLNIISELMERKLFSYIPIFE
                     AELESMLRPYDVFEKVLWQFLKKMSIFLQTKGNNQKEIENFIQSLQVLENPQLTSLFE
                     LRLQQYKALID"
     gene            1733..1837
                     /locus_tag="VYN69_001985"
     CDS             1733..1837
                     /locus_tag="VYN69_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011225312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEV1534192.1"
                     /translation="MENTEFSLELDVTEVATEQDYVSSGVTSTGCCKN"
BASE COUNT          622 a          294 c          354 g          568 t
ORIGIN      
        1 tgtcggtata aaaattttag tatatgaacc aaatttaaac gttagtatag ataacgtaat
       61 ttttgaaaag aattttgaac tatttacaaa acaaagcgat ctaattgttg caaatagatg
      121 ggataggggc cttgaagctt ataaagataa agtttacaca agggggatct ggataagaga
      181 ttaactattg atattaaaaa ggcaataatt tataaaattt gtacaaaaaa atgttaaatt
      241 ttcacatttt catagtatac tgtttaatag aaagtaggtt ctttgtaacc gctccattat
      301 aaaatccttt cactcttccg gtatactatt aggaaaaaca taactggagg atattttgag
      361 ctcacacctc atcgttccag tggctgttcc ccaagctcgt gtactgccca aaaaagttag
      421 acattctatt tagaacgagg attgagttct gtattgtaca aggctcggtc cttttagagt
      481 cagcttaagg ctggcttttt taatcaccaa agtgtcagaa tgttttgaca aatgaacaca
      541 aataatgata taatatgcaa aagctaggag gtggtaggat gattgaaaaa atggaactgg
      601 gggaatttta caaggaattg cgtttggcga gaaagctcaa gcagtcagat gtggcttgtg
      661 ctggactaac agcttctcag ctatccaagt tcgaactagg gcagtctatg ctgtctgcgg
      721 ataagctcat actagccatc caagggatca atgtgacctt tgatgagttt gggcacaagc
      781 tcaacaacta tcaagaatct ccacatatgc agattggtag aaaggttgtg gatcgctttg
      841 ctcatcaaga tatagctggc ttagagcaac tgttggagga agtcgagcaa ggacagatgg
      901 cggagaccta tcgtcgtttg aatgctattg tgattaaaaa tgcactccat tccttagata
      961 aaagctatcc gctagcagag gaggatagcg agtttttgac tacctacctc tatgctattg
     1021 agtcttggac ctggtttgaa ctctatcttt tttgcaatac catgcccttc ttgagcaatc
     1081 aagatctgat ctttttatca acctctttgc ttgaaaaatc caaggaattt aaagagttgg
     1141 tacataatag actgtatatg aaaagtggct ttctgaatat tatatcagag ctcatggagc
     1201 gcaagctttt ctcctatatc ccaatctttg aagcagagct ggagagcatg ttgcgtccgt
     1261 acgatgtttt tgaaaaagta ttgtggcaat ttttaaagaa aatgagtatt ttccttcaaa
     1321 ccaaaggaaa caatcaaaaa gagattgaaa attttatcca atctctgcaa gtattagaaa
     1381 atccacaatt aacatccctt tttgaactac gcttacagca atacaaagca cttatcgatt
     1441 agttagaaat gggaaaaaga taataatctc agcaacgatt gtcaataatg ttaaaacttg
     1501 tttcttcata tgacttccac tcctcattag taataacttt attataccag ggaaatgatc
     1561 aaatctacta aaatcgcaaa tgtgaaattt ttataagaaa aaatatcaaa tatgcgattt
     1621 tctaaaataa gccaattttc gtgttatact gtacttataa agcacttgaa gcaaatccta
     1681 ggtcgcagaa gtagtttata agtaaaaata attggaggag gaatcgttta ttatggaaaa
     1741 tacagaattt tcattggaac ttgatgtgac tgaagttgca actgaacaag attatgtttc
     1801 tagtggtgtt acatctacag gttgttgtaa aaactaat
//
LOCUS       DAVLVP010000155         1789 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.155, whole genome shotgun sequence.
ACCESSION   DAVLVP010000155 DAVLVP010000000
VERSION     DAVLVP010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1789)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.155"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..411)
                     /locus_tag="VYN69_001986"
     CDS             complement(<1..411)
                     /locus_tag="VYN69_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000358304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534193.1"
                     /translation="MDKKERQKIEQQRREMALTNTFFNRYLLLRYSIALFFFGNIYWL
                     LNQFISPSPIIIFPIMLIVFSILATIEQFKLYGNRKEKLGITLMFVRIQMLISIGLLV
                     LTWTSWFKNLFPIFENNQVARLFVFVVLLLGLVLS"
     gene            complement(442..756)
                     /locus_tag="VYN69_001987"
     CDS             complement(442..756)
                     /locus_tag="VYN69_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001070943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEV1534194.1"
                     /translation="MNQEELQVAAFEIILHSGNARSEIHEAFAKMREGSFDDAESKLN
                     QSNEIILEAHHAQTKLLQEYASGVEIKIEIIMVHAQDHLMTTMTLLEVAKEMLALYKK
                     VN"
     gene            complement(766..>1789)
                     /locus_tag="VYN69_001988"
     CDS             complement(766..>1789)
                     /locus_tag="VYN69_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001861534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEV1534195.1"
                     /translation="YNFSLDFQLIEGLITHLMTLQVRLESRITLNNPLVDEIKQNYSD
                     IFFMTREILANMDKFLEWTISDDEVAYVSLHFLAAMERSKESTKFNILAICATGFGAA
                     QMLRNRLETEFGKRVEVVDVIGYYELNQEKLKGIDFIVSAVDFSNLYFQIPVFKVSVF
                     LKSDEMEMIRKAMDQMQVSSYVQSSKINKFENNSFRQYFSKENFLICTESDKVNLLEK
                     MVEGLSVGESNEFEQSLLYGIKQREELSSVVFSEKIAVPHPIQPFGTEGKVSVAICKD
                     SLLWDNQSSHVQLVFLLSPSIYGNEGLATVTKKIVSLTENDELQNQLISCNNFEDFIN
                     IFEKIK"
BASE COUNT          599 a          338 c          232 g          620 t
ORIGIN      
        1 actcagaact aaacctaata gaagaactac aaaaacaaat aaacgtgcta cttggttatt
       61 ttcaaaaatc ggaaaaagat tcttaaacca acttgtccaa gttaaaacaa gtaatcctat
      121 tgaaataagc atttgtattc taacaaacat tagtgttatt cccaactttt ctttcctatt
      181 tccataaagt ttaaattgtt caatagttgc taaaatagaa aatactatga gcataatggg
      241 gaaaataata ataggcgagg gactaataaa ctgattcaaa agccaataaa tattcccaaa
      301 aaagaagagt gctattgaat aacgtagaag aagatatcga ttaaaaaaag tattagttag
      361 agccatctct cgacgttgtt gttcaatctt ttgtcgttct tttttatcca tatcatttcc
      421 tccttatata acaacacata tttagttaac tttcttataa agagctaaca tttcctttgc
      481 tacttctaat aatgtcatag tggtcattaa atgatcttga gcatgtacca tgataatttc
      541 aattttaatt tccactccac ttgcgtattc ttgcaagagt ttggtttgtg catgatgcgc
      601 ttcaagaatt atctcatttg attgatttaa tttactttct gcatcatcaa aactaccttc
      661 tctcattttt gcaaatgctt catgtatttc tgaccttgca tttcccgaat gcaggataat
      721 ttcaaatgct gcaacctgca gttcctcttg attcatatga acctcctatt ttatcttctc
      781 aaatatgtta ataaaatctt caaagttatt gcaagatatt agctgatttt gcaactcatc
      841 attctctgtc agagagacta tctttttagt cacagttgcc aaaccttcgt tcccatatat
      901 tgatggagat agaagaaata ctagctggac atgtgaactt tgattatccc agagtaacga
      961 atctttacag attgcaaccg aaacctttcc ctctgtacca aagggctgaa taggatgcgg
     1021 aactgcaatt ttttcagaaa aaacaactga acttaattct tcgcgctgtt taattccata
     1081 aagtaaagat tgttcaaact catttgattc accaacagat aaaccctcaa ccatcttttc
     1141 aagtaaattt accttgtctg attcagtaca tattaaaaaa ttttctttac taaaatactg
     1201 tctaaagctg ttgttttcaa atttgttaat ctttgatgat tgtacataac tagaaacttg
     1261 catctgatcc atagcttttc taatcatttc catctcatca ctcttaagaa acacactaac
     1321 tttaaaaact gggatttgaa aatatagatt tgagaaatca acagctgaca ctataaaatc
     1381 tattccttta agtttttctt gattcaattc ataatagcct attacatcaa caacttctac
     1441 tcgcttccca aactccgttt ccaaacgatt tcttaacatt tgggctgcac caaatcctgt
     1501 tgcacaaata gcaagaatat taaacttagt actctctttg ctacgttcca tagcagctaa
     1561 aaagtgaaga cttacatatg ctacttcatc atctgatatt gtccactcca agaacttgtc
     1621 catatttgca agaatttctc tagtcataaa gaatatatca ctataattct gtttaatttc
     1681 atctaccaaa gggttattta aggtaatccg gctttctaaa cgtacttgta gtgtcattag
     1741 atgagttatc aatccttcaa ttagttggaa atctaaagaa aagttatac
//
LOCUS       DAVLVP010000156         1772 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.156, whole genome shotgun sequence.
ACCESSION   DAVLVP010000156 DAVLVP010000000
VERSION     DAVLVP010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.156"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            358..834
                     /locus_tag="VYN69_001989"
     CDS             358..834
                     /locus_tag="VYN69_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003042293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEV1534196.1"
                     /translation="MIGKNIKSLRKTHDLTQPEFARIIGISRNSLSRYENGTSSVSTE
                     LIDIICQKFNVSYVDIVGEDKMLNPVEDYELTLKIEIVKERGANLLSRLYRYQDSQGI
                     SIDDESNPWILMSDDLSDLIQTNIYLVENFDEIERYSGYLDGIERMLEISEKRMVA"
     gene            834..1595
                     /locus_tag="VYN69_001990"
     CDS             834..1595
                     /locus_tag="VYN69_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000405360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEV1534197.1"
                     /translation="MEIQDYTDSEFKHALARNLRSLTRGKKSSKQPIAILLGGQSGAG
                     KTTIHRIKQKEFQGNIVIIDGDSFRSQHPHYLELQQEYGKDSVEYTKDFAGKMVESLV
                     TELSHLGYNLLIEGTLRTIDVPKETAQLLKNKGYEVQLALIATKPELSYLSTLIRYEE
                     LYIINPNQARATPKEHHDFIVNHLVDNTRQLEELAIFERIQIYQRDRSCVYDSKENTT
                     SAADVLQELLFGEWSQVEKEMLQVGEKRLNELLEK"
BASE COUNT          644 a          242 c          347 g          539 t
ORIGIN      
        1 acaacacttt attgaggtat tggatacgct ttgctaatag cctaataatc acatgtggag
       61 tgttgctaca acgaaaaagg tgataatcct tgatttcaag ctattttata agcattttgt
      121 ctttgtagat aaaggcaatt ttgacaataa aaatcctaaa aggtgaatcg ttatagatgt
      181 atttgtagat atcgtttgcg catcgaaaaa attaatacaa gaataaatat ttatggctct
      241 ttaggtgact ttcatagaag taaagtttag gatagaaaaa caagaaataa cgcaccattt
      301 ttggtgcgtt atgctttttt atgctataat ggatttataa aaataaagga gtttgctatg
      361 attggaaaga acataaaatc cttacgtaaa acacatgact taacacaacc tgaatttgca
      421 cgaattatag gaatttctcg aaatagcttg agtcgttatg aaaatggaac tagttcagtc
      481 tctacggaat taattgacat catttgtcag aagtttaatg tatcttatgt cgatattgta
      541 ggagaagata aaatgctcaa tcctgttgaa gattatgaat tgactttgaa aattgaaatt
      601 gtgaaagaaa gaggtgctaa tctattatct cgactctatc gttatcaaga tagtcaggga
      661 attagcattg atgatgaatc taatccttgg attttaatga gtgatgatct atctgacttg
      721 attcagacaa atatctattt agtagaaaat tttgatgaaa tagagagata tagcggctat
      781 ttggatggaa ttgaacgtat gttagagata tctgaaaagc ggatggtagc ttaatggaaa
      841 tccaagatta tactgatagt gaattcaaac atgctttagc aaggaatctt cgttcactga
      901 caagaggaaa aaagtccagt aaacaaccta tagcgatttt gcttggaggt caaagtggtg
      961 ccggtaagac tacaattcat cgtattaaac agaaagaatt tcaaggaaat attgttatca
     1021 tagatggtga tagttttcgt tctcagcatc cacactattt agaactgcag caagaatatg
     1081 gcaaagacag tgtagaatac accaaagatt ttgcaggtaa aatggtagag tctttagtaa
     1141 cagaattgag tcatttggga tacaatcttt tgatagaggg aactttacga acgattgacg
     1201 ttccaaagga aacagcacaa ctcttgaaaa ataagggata tgaagtacaa ttggccttaa
     1261 ttgcgacaaa gcctgaattg tcgtatctaa gtactcttat ccgttatgaa gaactgtaca
     1321 ttatcaatcc aaatcaagca cgcgcaactc caaaagaaca tcatgatttc attgtaaatc
     1381 atctagttga taatacacga caattggaag aactggctat ctttgaaaga attcaaattt
     1441 accaacgaga tagaagttgt gtatatgatt caaaagaaaa tacaacttca gcagcagatg
     1501 ttcttcaaga gttactcttt ggggagtgga gtcaggtaga gaaggagatg ttgcaggtgg
     1561 gggaaaagag acttaatgaa ttacttgaaa aataaacaat tgatattttt aggagaatag
     1621 aaatgagagg gtttaataac aagataaagt ctgtttatca agaactaaca aattccaaag
     1681 agaaattcgg tagctttcac aagactttaa ttcatttgca tacacctgtt tctcatgatt
     1741 acaagctatt ttctaattgg actgcaacga aa
//
LOCUS       DAVLVP010000157         1739 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.157, whole genome shotgun sequence.
ACCESSION   DAVLVP010000157 DAVLVP010000000
VERSION     DAVLVP010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1739
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.157"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..1139
                     /locus_tag="VYN69_001991"
     CDS             <1..1139
                     /locus_tag="VYN69_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001013545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEV1534198.1"
                     /translation="AIAISVLNFFIAERFSISRRGMVYFLTLEGISLYLLNFLVKKYW
                     KHVFFNLKNSKKILLLTVTKNMEKVLDKLLESDELSWKLVAVSVLDKSDFQHDKIPVI
                     EKEKIIEFATHEVVDEVFVNLPGESYDIGEIISRFETMGIDVTVNLKAFDKNLGRNKQ
                     IHEMVGLNVVTFSTNFYKTSHVISKRILDICGATIGLVLFAIASLVLVPLIRKDGGPA
                     IFAQTRIGKNGRHFTFYKFRSMRIDAEAIKEQLMDQNTMQGGMFKMDNDPRVTKIGRF
                     IRKTSLDELPQFWNVFIGDMSLVGTRPPTVDEYDQYTPEQKRRLSFKPGITGLWQVSG
                     RSKITDFDDVVKLDVAYIDNWTIWKDIEILLKTVKVVFIRDGAK"
     gene            1143..1592
                     /locus_tag="VYN69_001992"
     CDS             1143..1592
                     /locus_tag="VYN69_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--LPS N-acetylglucosamine
                     transferase"
                     /protein_id="HEV1534199.1"
                     /translation="MKVCLVGSSGGHLTHLYLLKPFWNDKERFWVTFDKADARSVLSE
                     EVVYPCYYPTNRNIKNLIKNTFLAYKILKKERPDLIISSGAAIAVPFFYIGKLFGSKT
                     VYIEVFDRIDASTLTGKLVYPVTDRFIVQWEEMKKVYPKAINLGGIF"
     gene            1592..>1739
                     /locus_tag="VYN69_001993"
     CDS             1592..>1739
                     /locus_tag="VYN69_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000578430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug MFS transporter"
                     /protein_id="HEV1534200.1"
                     /translation="MIFVTVGTHEQQFDRLIKEVDYLKKENLIQDEVFIQIGYSSYIP
                     KYCEW"
BASE COUNT          585 a          220 c          357 g          577 t
ORIGIN      
        1 ttgcaatagc tataagtgta ttaaactttt ttatagcgga acggtttagt atctctagaa
       61 gaggaatggt atacttctta actttagaag gaatatcctt atacttgtta aatttcttag
      121 taaagaaata ttggaagcat gtgtttttta atctaaaaaa tagcaagaaa attttactgt
      181 taacagtaac gaaaaatatg gaaaaagttc ttgataaatt gctagaatct gatgaacttt
      241 catggaaatt ggtagcagta agtgttttgg ataaatctga ttttcaacat gataaaatac
      301 ctgtaattga aaaggaaaaa attattgaat ttgcaacgca tgaagttgtg gatgaggtgt
      361 ttgtcaatct tccaggagag agctacgata ttggagaaat tatctctagg tttgagacaa
      421 tggggataga tgtaactgta aatcttaaag catttgataa gaatttgggt cgcaataaac
      481 aaattcatga gatggtagga ttgaatgtag tcactttctc tacaaatttt tataaaacta
      541 gtcatgtgat ttcaaagaga attctcgata tttgtggtgc cactattggc cttgttcttt
      601 ttgctatagc tagtctagtt ttagttccat tgattcgtaa agatggcgga ccagctattt
      661 ttgctcaaac tcgtataggg aaaaatggtc gacattttac cttttataaa ttccgttcga
      721 tgcggatcga tgctgaagct atcaaagaac aattgatgga tcaaaatacg atgcaaggtg
      781 gtatgtttaa gatggacaat gatcctcgtg ttacaaaaat tggtcgcttt attcgtaaaa
      841 ccagtttgga tgaattgccg cagttttgga atgtctttat aggagatatg agtttggtgg
      901 gaacacgtcc acctacagta gacgagtatg atcagtatac tccagaacag aaacgtcgac
      961 tcagctttaa acctggtatt acaggtttat ggcaggttag cggccgtagt aaaataaccg
     1021 attttgacga tgttgtaaaa ttagatgtgg cttatattga taattggaca atctggaaag
     1081 atattgaaat tttgcttaaa actgttaaag ttgtatttat aagagatgga gcgaagtaag
     1141 gaatgaaagt atgtctagtt ggatctagtg gaggccattt aactcatttg tatttattaa
     1201 aacctttctg gaatgataaa gaaagatttt gggttacttt tgataaggcg gacgctcgga
     1261 gtgtactctc tgaggaagtt gtatatccct gttattatcc aaccaatcgt aatataaaaa
     1321 atttaataaa aaatactttt ctcgcctata aaattttaaa aaaagagaga ccagatttga
     1381 ttatttcttc tggtgccgct atcgctgttc ctttctttta tattgggaag ttatttggat
     1441 caaagacagt gtatatagaa gtgtttgata gaattgatgc ttcaacactg acaggaaaac
     1501 ttgtttatcc agtaacggat aggtttattg tacaatggga agaaatgaaa aaagtatacc
     1561 caaaagcaat taacctagga gggatattct aatgattttt gtgactgtag gtacacacga
     1621 acaacaattt gatcgattga ttaaagaagt tgattatcta aaaaaagaaa atcttattca
     1681 agatgaagta tttattcaaa taggatattc cagttatatt ccgaaatatt gtgagtggg
//
LOCUS       DAVLVP010000158         1641 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.158, whole genome shotgun sequence.
ACCESSION   DAVLVP010000158 DAVLVP010000000
VERSION     DAVLVP010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.158"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            67..138
                     /locus_tag="VYN69_001994"
     tRNA            67..138
                     /locus_tag="VYN69_001994"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:99..101,aa:Gln,seq:ttg)
     gene            300..1502
                     /gene="rpsA"
                     /locus_tag="VYN69_001995"
     CDS             300..1502
                     /gene="rpsA"
                     /locus_tag="VYN69_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002897515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEV1534201.1"
                     /translation="MNEFEDLLNSVSQVETGDVVSAEVLTVDATQANVAISGTGVEGV
                     LTLRELTNDRDADINDFVKVGEVLDVLVLRQVVGKDTDTVTYLVSKKRLEARKAWDKL
                     VGREEEVVTVKGTRAVKGGLSVEFEGVRGFIPASMLDTRFVRNAERFVGQEFDTKIKE
                     VNAKENRFILSRREVVEAATAAARAEVFGKLAVGDVVTGKVARITSFGAFIDLGGVDG
                     LVHLTELSHERNVSPKSVVTVGEEIEVKILDLNEEEGRVSLSIKATVPGPWDGVEQKL
                     AKGDVVEGTVKRLTDFGAFVEVLPGIDGLVHVSQISHKRIENPKEALKVGQEVQVKVL
                     EVNADAERVSLSIKALEERPAQEEGQKEEKRVARPRRPRRQEKRDFELPETQTGFSMA
                     DLFGDIEL"
BASE COUNT          498 a          273 c          383 g          487 t
ORIGIN      
        1 gcggcggact gtaaatccgc tccttcgggt tcgggggttc gaatccctct ctctccattt
       61 cattaatggg gtatagccaa gcggtaaggc aagggacttt gactccctca tgcgttggtt
      121 cgaatccagc taccccagtt cttaggtaat aatcaagata aaaagcaaaa tatcttaggg
      181 tattttattt ttataattga aagacgtgaa tgatatgaac atgtccttgc gggtgcttag
      241 gaaaaaaatt ataagtatgt caagtttaag aaaaacttga ttgttggagg attttttaga
      301 tgaacgaatt tgaagatttg ctaaatagcg ttagtcaagt tgagactggt gatgttgtta
      361 gtgctgaagt attgacagtt gatgcgactc aagctaacgt tgcaatctct ggaactggtg
      421 ttgaaggtgt cttgactctt cgcgaattga caaacgatcg tgatgcagat atcaatgact
      481 ttgttaaagt aggagaagta ttggatgttc ttgtacttcg tcaagtagtt ggtaaagata
      541 ctgatacagt tacatacctt gtatctaaaa aacgccttga agctcgcaaa gcatgggaca
      601 aacttgttgg tcgcgaagaa gaagttgtta ctgttaaagg aacgcgtgcc gttaaaggtg
      661 gactttcagt agaatttgaa ggtgttcgtg gatttatccc agcttcgatg ttggatactc
      721 gtttcgtacg taacgctgag cgttttgtag gtcaagaatt tgatactaaa atcaaagaag
      781 ttaacgctaa agaaaaccgc ttcatccttt cacgtcgtga agttgttgaa gcagctactg
      841 cagcagctcg cgctgaagta ttcggtaaat tggctgttgg tgatgttgta actggtaaag
      901 ttgctcgtat cacaagcttc ggcgctttca tcgaccttgg tggtgttgat ggattggttc
      961 acttgactga attgtcacat gaacgtaacg tatcaccaaa atcagttgta actgttggtg
     1021 aagaaattga agtgaaaatc cttgatctta acgaagaaga aggacgtgta tcactttcta
     1081 ttaaagcaac agtaccagga ccatgggatg gcgttgagca aaaattggct aaaggtgatg
     1141 tagtagaagg aacagttaaa cgtttgactg acttcggtgc atttgttgaa gtattgccag
     1201 gtatcgatgg acttgttcac gtatcacaaa tttcacacaa acggattgaa aatccaaaag
     1261 aagctcttaa agttggtcaa gaagttcaag ttaaagttct tgaagttaac gcagatgcag
     1321 aacgcgtgtc actttctatt aaagctcttg aagaacgtcc agcccaagaa gaaggacaaa
     1381 aagaagaaaa acgtgttgct cgtccacgtc gtccaagacg tcaagaaaag cgtgatttcg
     1441 aacttccaga aacacaaaca ggattttcaa tggctgattt gtttggtgat atcgaacttt
     1501 aatcaaattg aaaattcaca aaatcctttg tttactaaac aaaggatttt tctggctctt
     1561 tgtcaactgt agtgggttga aaaaaagcta agctcgagaa aggacaaatt tcgtcctttc
     1621 ttttttgata ttcagagcga t
//
LOCUS       DAVLVP010000159         1578 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.159, whole genome shotgun sequence.
ACCESSION   DAVLVP010000159 DAVLVP010000000
VERSION     DAVLVP010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1578)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.159"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            2..892
                     /locus_tag="VYN69_001996"
     CDS             2..892
                     /locus_tag="VYN69_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000370602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534202.1"
                     /translation="MDNTKVIEWLNYCDQNDIKPWLWDFKNCYSSELTADNISNFLKY
                     KNGELVNPSTFCITKQVGSNADCDKEALPLYNVLGWQHSERDIIRGETLNSYITTFTQ
                     AITNDPNYKEICKKIGVNLNEYLNKQYPILHHNKNYQNFKMIQKNLKEFEAFAKLTHT
                     IGNFTVLPHWMNTGRYNFSKDYWDITMLSLQEWLTNLSPQAWKNFIDTYYLQPYVDNN
                     YQTEVFWETHNYEYIYPKKTEDFSIFLKRVNERIEERGKFIIKQICDKLNKKDFHFYK
                     EIENMDKIKYSNEFEQERKQ"
     gene            889..1359
                     /locus_tag="VYN69_001997"
     CDS             889..1359
                     /locus_tag="VYN69_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013851449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534203.1"
                     /translation="MTRVYKSIRLAYEAKVWIDELIQEKERKIQELNKEDFLDKLEKT
                     LLKNHYDELNGLSFNIILKASIGSVIEESYRNTKHYPIDKWQKLRQQMERDVKNVNPN
                     LETTVTPRIYLDEDVLAGLDDFRYVLMKEDCATRLPRLSYIIKLVVYSYWKEQH"
BASE COUNT          614 a          250 c          241 g          473 t
ORIGIN      
        1 tatggataac acaaaagtta ttgaatggtt aaattactgt gaccaaaacg atatcaaacc
       61 atggttatgg gattttaaaa attgttactc tagcgagtta accgcagata atatttcaaa
      121 ttttctaaaa tataaaaatg gagaactcgt aaatccttcc acattttgta tcacaaaaca
      181 ggttggtagt aacgctgact gcgataaaga ggcactccca ctttataatg ttttaggttg
      241 gcaacattcc gagagagata taataagagg tgagacctta aattcttata taactacatt
      301 tactcaagct attaccaatg acccgaatta taaagaaatt tgtaaaaaga ttggagtaaa
      361 cttaaatgaa tatctaaaca agcagtatcc aatcttacac cataataaaa actatcaaaa
      421 tttcaagatg attcaaaaaa acctaaaaga atttgaagca ttcgcaaaat taacacatac
      481 aatcggaaac tttactgtat tgcctcattg gatgaatact ggacgataca atttctcaaa
      541 agattactgg gatattacaa tgctttctct tcaagaatgg cttactaatt tatctcctca
      601 agcatggaaa aattttattg atacatacta tcttcaaccc tatgttgata ataactatca
      661 aacagaggta ttttgggaaa ctcataacta cgaatacatt tatcccaaga aaaccgaaga
      721 tttctccatc ttcctaaaaa gagtcaatga acggattgag gagcgcggga aattcatcat
      781 taaacaaata tgcgataaac taaataaaaa agatttccat ttctataagg aaattgaaaa
      841 tatggataaa attaaatact caaatgaatt tgaacaagag aggaaacaat gactagagta
      901 tataaaagca tccgccttgc gtacgaggca aaggtatgga tagatgaatt gattcaagaa
      961 aaagaacgta aaattcaaga acttaataaa gaggattttc tagataaact tgaaaaaaca
     1021 cttcttaaaa atcactatga tgaattgaat ggtctttcat ttaatatcat tctaaaagcc
     1081 tcaataggga gtgtcataga agaatcctat cgaaatacta aacactatcc aattgacaaa
     1141 tggcaaaagc tacgtcagca gatggaaagg gacgtaaaaa atgttaatcc taacttagaa
     1201 accacagtta ctccccgtat ttatctagac gaggatgtgc ttgctgggct tgatgatttt
     1261 cgctatgttt tgatgaaaga ggactgtgct actagactac ctcgtcttag ctacattatc
     1321 aaacttgttg tatattccta ctggaaagaa caacactaat ctaatcagtt ctactgttta
     1381 gattttcatt tctttaaaat gtatatttgt tgtactacta ataaatctag aaaaataggt
     1441 aatctatgtt taaattaaag aaagtcgctg acttaaaaag aaattatatc atttcaacta
     1501 acaaattaaa ggtaactcat tggtcaatat taagcctctc tatctgcatt atgcttacta
     1561 ttattgcaaa tattagag
//
LOCUS       DAVLVP010000160         1566 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.160, whole genome shotgun sequence.
ACCESSION   DAVLVP010000160 DAVLVP010000000
VERSION     DAVLVP010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1566
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.160"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            351..1046
                     /locus_tag="VYN69_001998"
     CDS             351..1046
                     /locus_tag="VYN69_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001144916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1534204.1"
                     /translation="MPVNEYGQMIGESMEGYTPGALPSIDFLEGRYARIEALSVEKHA
                     EDLLAVYGPDTPREMWTYLFQEPVADMGELVSLLNQMLARKDRFYYAIIDKATGKALG
                     TFSLMRIDQNNRVIEVGAVTFSPELRGTRIGTEAQYLLARYIFEELNYRRYEWKCDAL
                     NLPSRRVAERLGFVYEGTFRQAVVYKGRTRDTDWLSMIDMDWPQVKDRLETWLRPENF
                     DKNGQQYKSLREL"
     gene            1058..1474
                     /locus_tag="VYN69_001999"
     CDS             1058..1474
                     /locus_tag="VYN69_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001862593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1534205.1"
                     /translation="MITIKKQEIVKLEDVLHLYQAVGWTNYTHQPEMLEHALSHSLVI
                     YLALDGDAVVGLIRLVGDGFSSVFVQDLIVLPIYQRQGIGSALMKEALKDYKDAYQVQ
                     LVTEETEKNVGFYRSMGFEILSTYNCIGMTWANREK"
BASE COUNT          488 a          243 c          373 g          462 t
ORIGIN      
        1 tagataaact tactgattct gaatcgaaaa aatacggtga atatattgat ttgtacgaat
       61 cgtgtcgtca gaacaattta agtgttctac ttaactttct tcaaaattcg gatgataaac
      121 gaggagttat tgctagtaac tcagtgagaa ttatgtttga atctttggat aaagcagacc
      181 aaattgcggc cctgaatatt tgtagaaact atgtagcacg agaaaatgtt ttaaataaag
      241 aagatgcaga aaattgttaa atgaaattga taaaaaactt gaaattagca gataggacaa
      301 ttcaaataga gttgagtaaa aagtttgaaa agtgaaacaa ggagaaagct atgccagtaa
      361 atgaatatgg tcagatgatt ggtgagtcaa tggaaggtta tacacccggt gcactgcctt
      421 ctattgattt cttagaaggg cgttatgcta ggatagaggc tctctcggta gaaaaacatg
      481 cggaggattt gctagctgtt tatggcccgg atactcctcg ggagatgtgg acctatcttt
      541 ttcaggagcc agtagcagat atgggggagc tggttagcct tttaaatcag atgttggctc
      601 gtaaggaccg tttttactat gcaatcatag acaaggcgac tggtaaggct ttgggaactt
      661 tttctctcat gcgcattgac cagaataacc gagtaataga agtcggagca gtcacttttt
      721 ctccagagct aaggggtaca cggataggaa ctgaggctca gtatctccta gctcgctata
      781 tttttgagga gctaaactat cgtcgctacg agtggaaatg cgatgcctta aatctgccat
      841 ccagacgagt tgcggagcgt ttgggatttg tctatgaagg aaccttccgc caggcggtgg
      901 tttataaggg gcgtacgaga gatacggatt ggttgtctat gattgatatg gactggcctc
      961 aagtcaaaga tcgtttggaa acatggctgc gtcctgaaaa ttttgataaa aatggacagc
     1021 agtacaagag cttgagagaa ctttaagagg tgttgagatg attactatta aaaagcaaga
     1081 aattgtcaag ctagaggatg ttttgcatct ctatcaggct gtcggttgga caaattatac
     1141 ccatcaacca gagatgctgg agcatgcctt atcgcattca ttagtaattt atctggcact
     1201 tgatggtgat gctgtggtgg gcttgattcg tttggttgga gatggattct catcagtttt
     1261 tgtacaggat ttgattgttt taccaatcta tcagcgtcaa gggattggta gtgccctaat
     1321 gaaagaggcc ttaaaagatt acaaagatgc ttatcaagtc cagctggtga cagaagagac
     1381 agaaaaaaac gtgggatttt atcgttctat gggctttgaa atcttatcca cctataattg
     1441 tatagggatg acttgggcga atcgagaaaa ataacaaaac ttgttttttc ttaagcaaaa
     1501 tttaaggatg gtctagtatt atataatcat taaataaaga cctcctaact ttatttaata
     1561 aaatcc
//
LOCUS       DAVLVP010000161         1559 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.161, whole genome shotgun sequence.
ACCESSION   DAVLVP010000161 DAVLVP010000000
VERSION     DAVLVP010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1559
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.161"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            60..977
                     /gene="adcAII"
                     /locus_tag="VYN69_002000"
     CDS             60..977
                     /gene="adcAII"
                     /locus_tag="VYN69_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding lipoprotein AdcAII"
                     /protein_id="HEV1534206.1"
                     /translation="MKKQNLFLVLLSVFLLCLGACGQKESQTGKGMKIVTSFYPIYAM
                     VKEVSGDLNDVRMIQSSSGIHSFEPSANDIAAIYDADVFVYHSHTLESWAGSLDPNLK
                     KSKVKVLEASEGMTLERVPGLEDVEAGDGVDEKTLYDPHTWLDPEKAGEEAQIIADKL
                     SEVDSEHKETYQKNAQAFIKKAQELTKKFQPKFEKATQKTFVTQHTAFSYLAKRFGLN
                     QLGIAGISPEQEPSPRQLTEIQEFVKTYKVKTIFTESNASSKVAETLVKSTGVGLKTL
                     NPLEADPQNDKTYLENLEENMSVLAEELK"
     gene            985..>1559
                     /locus_tag="VYN69_002001"
     CDS             985..>1559
                     /locus_tag="VYN69_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1534207.1"
                     /translation="MKINKKYLAGSVAVLALSVCSYELGRHQAGQVKKESNRVSYIDG
                     DQAGQKAENLTPDEVSKREGINAEQIVIKITDQGYVTSHGDHYHYYNGKVPYDAIISE
                     ELLMKDPNYQLKDSDIVNEIKGGYVIKVDGKYYVYLKDAAHADNIRTKEEIKRQKQEH
                     SHNHNSRADNAVAAARAQGRYTTDDGYIFNAS"
BASE COUNT          537 a          262 c          344 g          416 t
ORIGIN      
        1 agattaaatt ttcaggagta gaatatttac tagttaatta aatggttaag gagttgttca
       61 tgaagaaaca aaatttattt ttagtcctgt taagtgtctt tcttttgtgc ttgggggctt
      121 gtggtcaaaa ggaaagtcag acaggaaagg ggatgaaaat tgtgaccagt ttttatccta
      181 tctacgctat ggttaaggaa gtatctggtg acttgaatga tgttcggatg attcagtcaa
      241 gtagtggtat tcactccttt gaaccttcgg caaatgatat cgcagccatc tatgatgcag
      301 atgtctttgt ttaccattct catacactcg aatcttgggc aggaagtctg gatccaaatc
      361 taaaaaaatc caaagtgaag gtcttagagg cttctgaggg aatgaccttg gaacgtgtcc
      421 ctggactaga ggatgtggaa gcaggggatg gagttgatga aaaaacgctc tatgaccctc
      481 acacatggct agatcctgaa aaagctggag aagaagccca aattatcgct gataaacttt
      541 cagaggtgga tagtgagcat aaagagactt atcaaaaaaa tgcgcaagcc tttatcaaaa
      601 aagctcagga attgactaag aaattccaac caaaatttga aaaagcgact cagaaaacat
      661 ttgtaacaca acatacagcc ttttcttatc tagcgaagag atttgggctt aatcaacttg
      721 gtattgcagg catctctcct gaacaagaac caagtccacg acaattaaca gaaattcagg
      781 aatttgttaa aacctataag gttaaaacga tttttacaga aagtaatgct tcttcaaaag
      841 tagctgaaac tcttgtcaaa tcaacaggtg tgggtcttaa aactctgaat cctttagagg
      901 cagacccaca aaatgacaag acctatttag aaaatctgga agaaaatatg agtgttctag
      961 cagaagaatt aaagtgagga aagaatgaaa atcaataaaa aatatctagc aggttcagtg
     1021 gcagtccttg ccctaagtgt ttgttcctat gaacttggtc gtcaccaagc tggtcaggtt
     1081 aagaaagagt ctaatcgagt ttcttatata gatggtgatc aggctggtca aaaggcagaa
     1141 aatttgacac cagatgaagt cagtaagaga gaggggatca acgccgaaca aattgttatc
     1201 aagattacgg atcaaggtta tgtgacctct catggagacc attatcatta ctataatggc
     1261 aaggttcctt atgatgccat catcagtgaa gaacttctca tgaaagatcc gaattatcag
     1321 ttgaaggatt cagacattgt caatgaaatc aagggtggct atgtgattaa ggtagacgga
     1381 aaatactatg tttaccttaa agatgcggcc catgcggaca atattcggac aaaagaagag
     1441 attaaacgtc agaagcagga acacagtcat aatcataact caagagcaga taatgctgtt
     1501 gctgcagcca gagcccaagg acgttataca acggatgatg ggtatatctt caatgcatc
//
LOCUS       DAVLVP010000162         1551 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.162, whole genome shotgun sequence.
ACCESSION   DAVLVP010000162 DAVLVP010000000
VERSION     DAVLVP010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1551)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1551)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1551
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.162"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            19..1365
                     /gene="gdhA"
                     /locus_tag="VYN69_002002"
     CDS             19..1365
                     /gene="gdhA"
                     /locus_tag="VYN69_002002"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011999806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEV1534208.1"
                     /translation="MTSAKEYIQSVFETVKARNGHEAEFLQAVEEFFNTLEPVFEKHP
                     EYIEENILARITEPERVISFRVPWVDRDGKVQVNRGYRVQFNSAVGPYKGGLRFHPTV
                     NQGILKFLGFEQIFKNVLTGLPIGGGKGGSDFDPKGKTDAEVMRFCQSFMTELQKHIG
                     PSLDVPAGDIGVGGREIGYLYGQYKRLNQFDAGVLTGKPLGFGGSLIRPEATGYGLVY
                     YTEEMLKANGNSFAGKKVVISGSGNVAQYALQKATELGATVISVSDSNGYVIDENGID
                     FDLLVDVKEKRRARLTEYAAEKATATYHEGSVWTYAGNYDIALPCATQNEINGEAAKR
                     LVAQGVICVSEGANMPSNLDAIKVYKENGIFYGPAKAANAGGVAVSALEMSQNSLRLS
                     WTREEVDGRLKDIMTNIFNTAKTTSETYGLDKDYLAGANIAAFENVANAMIAQGVV"
BASE COUNT          467 a          307 c          327 g          450 t
ORIGIN      
        1 caataaaagg agaacgatat gacatctgct aaagaatata tccaaagcgt gtttgaaact
       61 gtgaaagctc gtaacgggca cgaggctgaa ttcctccaag ctgttgaaga atttttcaac
      121 actttggaac ctgtatttga aaaacaccct gagtatatcg aagaaaatat cttggcacgt
      181 attactgagc ctgagcgcgt gatttctttc cgtgttcctt gggttgaccg tgatggaaaa
      241 gttcaagtaa accgcggcta ccgtgttcaa ttcaactcag ctgttggacc atataaaggc
      301 ggacttcgtt tccacccaac tgtaaaccaa gggattttga aattcctcgg atttgaacaa
      361 atctttaaaa acgtcttgac tggacttcct atcggtggag gtaaaggtgg atcagacttc
      421 gatcctaaag gtaaaacaga tgctgaagtg atgcgcttct gccaaagctt catgactgaa
      481 ttgcaaaaac acattggacc atcacttgac gtacctgctg gtgatatcgg tgttggtgga
      541 cgtgagattg gttaccttta cggtcaatac aagcgtctta accaatttga tgctggtgtc
      601 ttgactggta aacctcttgg atttggtggt agcttgattc gtccagaagc aactggttac
      661 ggtttggttt actatactga agaaatgctc aaagctaatg gtaacagctt tgctggtaag
      721 aaagtggtta tttcaggttc tggtaacgtt gctcaatacg ctcttcaaaa agcaaccgaa
      781 cttggtgcaa cagttatctc tgtttctgac tcaaacggct atgttatcga tgaaaacggt
      841 atcgacttcg atcttttggt tgatgttaaa gaaaaacgtc gcgctcgttt gactgagtat
      901 gcagctgaga aagcaactgc tacttatcat gaaggttctg tatggactta cgctggaaac
      961 tatgacattg cgcttccatg tgctactcaa aacgaaatca acggtgaagc agctaaacgt
     1021 ttggttgctc aaggcgttat ctgtgtatct gaaggtgcca acatgccaag caaccttgat
     1081 gccatcaaag tttacaaaga aaatggtatc ttctacggac ctgccaaagc tgccaacgct
     1141 ggtggtgtag ccgtttcagc ccttgaaatg agccaaaaca gccttcgcct ctcatggact
     1201 cgtgaagaag ttgatggacg tctcaaagac atcatgacca acatctttaa cacagctaaa
     1261 acaacttcag aaacatatgg tcttgataaa gactaccttg caggagctaa cattgctgcc
     1321 tttgaaaatg tagcaaacgc tatgattgca caaggcgtgg tataaaaaaa aattacggaa
     1381 actgactacc cagacgggta gccagttttt tcaattccta aaatttaaga ttctaatacc
     1441 ctttatgcaa aaatatagaa ttctaccttt ttctttatat aggctaatgt ttttaatata
     1501 tttattgtct atttttgaaa agacaataaa actattctac tatagattat c
//
LOCUS       DAVLVP010000163         1520 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.163, whole genome shotgun sequence.
ACCESSION   DAVLVP010000163 DAVLVP010000000
VERSION     DAVLVP010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1520)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1520)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1520
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.163"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            162..1340
                     /locus_tag="VYN69_002003"
     CDS             162..1340
                     /locus_tag="VYN69_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000175872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HEV1534209.1"
                     /translation="MTLSKKQLQLRAKILETVYTLGPISRIEIATKTGITPATTSSIT
                     NDLIKENILLELGEDEHDTSVGRKKILLDIQAKRFYYIGCELSEKHFTFALGDNLGNI
                     LKEEKEIVTKQLIQEKGNQLINQTLKQFLNNCSDYEIEAIGIALPGRYLDDYKITTNN
                     PLWQHIDLEMIQSHFDKPLFFSNNVNCMAIGKRLFSRQQNDPNFAYFHFARGMHCSYI
                     YDGNIYGKGNLMIGEIGHTVVSSEGEECSCGRKGCLQTFAGESWLIKKSKILYHQSPY
                     SLLPSLVKNADDIDIQVILTAYQLGDTGIITLIHQALLYLSQTILNISMMIDSQKIYL
                     HSPLLTNQHIIQKLYSEMNYKPKLLYNRLPEVIIESYNDFTAAHSAIALCLYHTILHS
                     "
BASE COUNT          566 a          265 c          221 g          468 t
ORIGIN      
        1 ttgtcaagga tttgtgtaaa catttgatta ttttttatta tcaatatata ataaaaagag
       61 accagtaagt aatgaaaagc ttaaaaagat taacatctac atttatccat agcaaaaaat
      121 cataggattt taagaatctg acaactagga agaaaactaa tatgacctta tctaagaaac
      181 aattacaact aagagctaaa atactggaaa cagtttacac attaggtccc atttcacgaa
      241 ttgaaatcgc tactaaaaca ggaataacac ctgcaacaac tagcagtatc acaaatgatc
      301 tgattaaaga aaatattctc ttagaacttg gtgaagacga acacgataca agcgtaggga
      361 gaaaaaaaat cctgctagac attcaagcta aacgatttta ctacattggt tgtgaactat
      421 ctgaaaaaca tttcactttt gctttaggag ataatctagg aaatatctta aaggaagaga
      481 aagaaattgt tactaaacag ttaatccaag aaaagggaaa tcaactcatt aaccagacac
      541 taaagcaatt cttaaataac tgctctgatt atgaaataga agcaattgga atcgcgctac
      601 ctggtcgcta tttagatgat tataaaataa caacaaacaa tcctttatgg caacatatcg
      661 acttagaaat gattcaatcc cactttgata agcctttgtt tttctctaac aatgttaact
      721 gtatggctat aggtaaacgc ctatttagtc gccaacagaa tgaccctaat tttgcttatt
      781 ttcattttgc tagaggtatg cactgttctt atatctacga cggaaacatt tatgggaaag
      841 gaaatctaat gattggagaa atagggcata cagttgtttc atctgaggga gaagaatgta
      901 gctgcgggcg caagggatgc ttacaaactt ttgctggaga atcttggtta atcaaaaaat
      961 caaaaattct atatcatcaa tctccctatt ctctacttcc tagtttagtc aaaaatgccg
     1021 atgatattga tatccaagtt attttaaccg cttatcaatt aggtgacact ggaataatca
     1081 cacttatcca tcaagcctta ctctatctta gtcaaaccat tttaaatatc agtatgatga
     1141 ttgactctca aaaaatctac ctacatagtc ctttactaac aaatcaacac atcatccaaa
     1201 aactatatag tgaaatgaac tacaaaccca aactgctcta taatcgccta cctgaagtca
     1261 ttattgaatc ttacaatgat tttacagccg cacactcagc tattgcactt tgtttgtatc
     1321 atacaattct acattcttaa ttttctatta catttaataa ataggctaaa aagagggagt
     1381 atatcataaa aagcgaataa acctgttttc tgaaaatcag aattctgttt tgttcgcttt
     1441 ctttatattt ggaattatac tcctatctcc catttttcat aatgtatagt aaaatgaaat
     1501 aataacagga cgaatcgatc
//
LOCUS       DAVLVP010000164         1488 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.164, whole genome shotgun sequence.
ACCESSION   DAVLVP010000164 DAVLVP010000000
VERSION     DAVLVP010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.164"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..1325)
                     /locus_tag="VYN69_002004"
     CDS             complement(<1..1325)
                     /locus_tag="VYN69_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015898732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec-dependent serine-rich glycoprotein
                     adhesin"
                     /protein_id="HEV1534210.1"
                     /translation="MFKVLRGGVDTTQVMTETVEDKVSHSITGLDILKGIVAAGTVIS
                     GTVATQTKVFTNESAVLEKTVEKTDALATNGTVVLGTISTSNSASSTSLSASESASTS
                     ASESASTSASTSASTSASESASTSASTSISASSTVVGSQTAAATEATAKKVEEDRKKP
                     ASDYVASVTNVNLQSYAKRRKRSVDSIEQLLASINAAVFSGNMIVNGAPAINASLNIA
                     KSETKVYTGTGKDSFYNIPIYYQLTVTNDGSKLTFTYTVTYVNPTTKILGNLSKMRLG
                     YSIYNTGTSIQTMLTLGSGLGTPSDVKNYITDKDGVLVRSYNTSTMTTQGSGYTWANG
                     AQMNGWQARREYGLTSSWTVPITGTDTSFTFTPYAAKTDNIRTNYFNGRGKVVESSTT
                     SQSLSQSKSLSVSASQSASASASTSASASASTSASASASTSASASASTSA"
     gene            complement(1323..1436)
                     /locus_tag="VYN69_002005"
                     /pseudo
     CDS             complement(1323..1436)
                     /locus_tag="VYN69_002005"
                     /inference="COORDINATES: protein motif:HMM:TIGR03715.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="KxYKxGKxW signal peptide domain-containing
                     protein"
BASE COUNT          402 a          316 c          301 g          469 t
ORIGIN      
        1 gcgcttgtgc ttgctgaagc cgacgcactc gttgatgctg aggctgacgc actcgttgat
       61 gctgaagctg aggcactggt tgatgctgag gctgaggcgc tttgactagc acttactgag
      121 agtgacttag actgtgaaag tgactgactg gtcgtgctag attcaactac ctttcctcta
      181 ccattgaagt agttagttct aatgttatct gttttagcag cgtaaggggt aaatgtaaag
      241 gatgtatccg ttccagtaat tggtacagtc caagatgatg ttaatccata ttctctccta
      301 gcctgccaac cattcatttg ggcaccattt gcccaagtat acccactacc ctgcgtcgtc
      361 attgtagatg tattatagga tcgaaccagt acaccatctt tgtcagtaat gtagttcttt
      421 acatctgaag gggtaccaag accactgcca agggttaaca ttgtttgtat tgaagtacct
      481 gtattataga tagaataccc aagacgcatc tttgaaagat taccaagaat ctttgttgta
      541 ggattcacat acgtaaccgt ataggtaaag gtcaattttg aaccatcatt tgtcactgtc
      601 aattgatagt aaattggaat attataaaat gaatctttac ctgtacccgt gtaaactttt
      661 gtctcacttt tagcaatgtt tagacttgca ttaattgcag gagcgccatt tacaatcata
      721 ttgccagaaa aaacagcagc atttatagaa gccagcaatt gctcgatgga atccactgaa
      781 cgcttgcgtc gcttagcata agattggaga ttgacatttg taactgatgc tacataatca
      841 ctagctggtt tcttacgatc ttcttcgacc ttcttagcag ttgcttctgt agcggcagct
      901 gtttgtgaac ctaccacagt agatgatgca gagatacttg tcgaagccga tgtacttgct
      961 gattctgatg cacttgtact cgctgaggtc gaagcgctgg ttgaggctga ctcagatgca
     1021 gatgtacttg ccgactctga agctgacaaa ctagttgaac tcgctgaatt acttgtagat
     1081 atcgtaccta gaactactgt accatttgtt gccaaagcat ccgttttctc tacagttttt
     1141 tcaagtactg ctgactcatt tgtaaatacc ttcgtttgag ttgcaacggt tccacttatg
     1201 acagttcccg cagcaactat ccccttgagg atatcaagcc cagtaattga atgacttact
     1261 ttatcttcta ccgtttcggt catgacctga gtagtatcaa caccacctcg caagacctta
     1321 aacaagccaa aaagagaagt cgaggcccgt agccaatgct tccctgattt gattaatttg
     1381 aaccgagtta ctcggtctgt ttctctatat tcacccttct gacgtctaaa aaatattttg
     1441 aaatttcctt tcgagtatta ttttcatgta tggatataaa taaattac
//
LOCUS       DAVLVP010000165         1435 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.165, whole genome shotgun sequence.
ACCESSION   DAVLVP010000165 DAVLVP010000000
VERSION     DAVLVP010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1435
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.165"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..710)
                     /locus_tag="VYN69_002006"
     CDS             complement(<1..710)
                     /locus_tag="VYN69_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534211.1"
                     /translation="MKDDQKYLLAGLYSLLVAIFYFPLIESKGIFVSILMAVLLLYLI
                     YFIATVIHIVIIKFIRKKSFKYLVLYPFTYDGSWRFQPINLLYFPEMVRDVIPINLVQ
                     EYCQGQPYGLLKKMLKRIRLSREISLLLATIIVYFFTHRILPLSVFTFMFSYILLFAQ
                     SYLGGNTVWIGNRRLIIDDEFEKILLSKSYVKEISSARYSEYLTCEYKNLTPIILLAI
                     FENLLDSYLLQNQSKVDL"
BASE COUNT          548 a          260 c          181 g          446 t
ORIGIN      
        1 tctaagtcaa ccttactctg attctgaagc aagtaggagt caagcaaatt ttcaaaaata
       61 gctaacaata tgattggtgt aaggtttttg tattcacatg tcaagtattc cgagtatcgt
      121 gcagatgata tttcttttac ataacttttt gaaagcaaga ttttttcaaa ttcatcatct
      181 attattaatc ttctatttcc aatccaaacg gtattaccac ccaaataaga ttgtgcaaat
      241 agcaatatgt aagaaaacat gaacgtaaat actgaaagag gtagaattct atgcgtaaaa
      301 aaatagacga taatggtagc caggagtaaa gatatctctc gtgacaatcg aattcttttc
      361 agcatctttt ttaacagtcc gtaaggttgt ccttggcaat attcttgaac taaattaatc
      421 ggaatgacat ctctcaccat ttctggaaag tagagtaagt ttataggttg aaatctccag
      481 ctcccatcat aagtaaatgg atatagaacc aaatacttaa aagatttttt tcttataaac
      541 ttaattatca caatatgaat gactgttgca atgaagtata ttaaatataa aagtaaaacc
      601 gccatcaata tacttacaaa aatccccttg gactcaatca atggaaaata aaatattgca
      661 actagaagcg aataaagtcc cgctaataaa tatttctgat catctttcat tcaaaacacc
      721 atattattta ctaagaatac atcaatacaa tagagcaact ctgtaagtca atatttgata
      781 aagcaaagct ttaaaccctt ccccagacgt tagcttttct tcaacccact acagttgaca
      841 aggagcccaa attattgata aaaactttag tcatcatcgt ctctataaaa atattttcta
      901 tacactcttg ataataccaa aattaatgcc aatatcatac caagagctat aataatagaa
      961 tttattagat ttattttagt atatccaatc actagtccaa taattggact agcagacatg
     1021 cttagatata ataaaaataa caaaaaatga atttcaccaa ttcctgcaaa aaaatgtttc
     1081 atagctatag ttttacgccc ataaataccc atcaaattta ccgtctacga tatctaaacc
     1141 atatgcaatg gctcctccta atgagaagac ttcagcaaca gtagccgcaa tattaagagc
     1201 attagtaata gttgccatac taactccacc aatatatctt gttatagcac ttagttacct
     1261 agataagata tcctttagct tgcttccaaa atttaagtct acacctataa tatgagttca
     1321 tattagtact gctaaatcct atctatagta atgtcaaaca cctaaataaa gtcatctctc
     1381 caattcattc aagaaatgaa gaaaggcaaa aaatctttaa aataagaaaa atgca
//
LOCUS       DAVLVP010000166         1415 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.166, whole genome shotgun sequence.
ACCESSION   DAVLVP010000166 DAVLVP010000000
VERSION     DAVLVP010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1415
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.166"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..>1415
                     /gene="pcpA"
                     /locus_tag="VYN69_002007"
     CDS             <1..>1415
                     /gene="pcpA"
                     /locus_tag="VYN69_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000753868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="choline-binding protein PcpA"
                     /protein_id="HEV1534212.1"
                     /translation="KTTILSLTTAAVILAAYVPNEPILADTPSSEVIKETKVGSIIQQ
                     NNIKYKVLTVEGNIGTVQVGNGVTPVEFEAGQDGKPFTIPTKITVGDKVFTVTEVASQ
                     AFSYYPDETGRIVYYPSSITIPSSIKKIQKKGFHGSKAKTIIFDKGSQLEKIEDRAFD
                     FSELEEIELPASLEYIGTSAFSFSQKLKKLTFSSSSKLELISHEAFANLSNLEKLTLP
                     KSVKTLGSNLFRLTTSLKHVDVEEGNESFASVDGVLFSKDKTQLIYYPSQKNDESYKT
                     PKETKELASYSFNKNSYLKKLELNEGLEKIGTFAFADAIKLEEISLPNSLETIERLAF
                     YGNLELKELILPDNVKNFGKHVMNGLPKLKSLTIGNNINSLPSFFLSGVLDSLKEIHI
                     KNKSTEFSVKKDTFAIPETVKFYVTSEHIKDVLKSNLSTSNDIIVEKVDNIKQETDVA
                     KPKKNSNQGVVGWVKDKGLWY"
BASE COUNT          550 a          197 c          230 g          438 t
ORIGIN      
        1 aaaaactaca atattatcat taactacagc tgcggttatt ttagcagcat atgtccctaa
       61 tgaaccaatc ctagcagata ctcctagttc ggaagtaatc aaagagacta aagttggaag
      121 tattattcaa caaaataata tcaaatataa ggttctaact gtagaaggta acataggaac
      181 tgttcaagtg ggtaatggag ttactcctgt agagtttgaa gctggtcaag atggaaaacc
      241 attcacgatt cctacaaaaa tcacagtagg tgataaagta tttaccgtta ctgaagtagc
      301 tagtcaagct tttagttatt atccagatga aacaggtaga attgtctact atcctagctc
      361 tattactatc ccatcaagca taaaaaaaat acaaaaaaaa ggcttccatg gaagtaaagc
      421 taaaactatt atttttgaca aaggcagtca gctggagaaa attgaagata gagcttttga
      481 tttttctgaa ttagaagaga ttgaattgcc tgcatctcta gaatatattg gaacaagtgc
      541 attttctttt agtcaaaaat tgaaaaagct aaccttttcc tcaagttcaa aattagaatt
      601 aatatcacat gaggcttttg ctaatttatc aaatttagag aaactaacat taccaaaatc
      661 ggttaaaaca ttaggaagta atctatttag actcactact agcttaaaac atgttgatgt
      721 tgaagaagga aatgaatcgt ttgcctcagt tgatggtgtt ttgttttcaa aagataaaac
      781 ccaattaatt tattatccaa gtcaaaaaaa tgacgaaagt tataaaacgc ctaaggagac
      841 aaaagaactt gcatcatatt catttaataa aaattcttac ttgaaaaaac tcgaattgaa
      901 tgaaggttta gaaaaaatcg gtacttttgc atttgcagat gcgattaaac ttgaagaaat
      961 tagcttacca aatagtttag aaactattga acgtttagcc ttttacggta atttagaatt
     1021 aaaagaactt atattaccag ataatgttaa aaattttggt aaacacgtta tgaacggttt
     1081 accaaaatta aaaagtttaa caattggtaa taatatcaac tcattgccgt ccttcttcct
     1141 aagtggcgtc ttagattcat taaaggaaat tcatattaag aataaaagta cagagttttc
     1201 tgtgaaaaaa gatacatttg caattcctga aactgttaag ttctatgtaa catcagaaca
     1261 tataaaagat gttcttaaat caaatttatc tactagtaat gatatcattg ttgaaaaagt
     1321 agataatata aaacaagaaa ctgatgtagc taaacctaaa aagaattcta atcagggagt
     1381 agttggttgg gttaaagaca aaggtttatg gtatt
//
LOCUS       DAVLVP010000167         1391 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.167, whole genome shotgun sequence.
ACCESSION   DAVLVP010000167 DAVLVP010000000
VERSION     DAVLVP010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1391
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.167"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            88..1266
                     /locus_tag="VYN69_002008"
     CDS             88..1266
                     /locus_tag="VYN69_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534213.1"
                     /translation="MTLPVRKSLHDAVLQASKADTWEQATKEWNEVSLMFNGIGRSNC
                     VCGNAIKYAYELFNGVTGQRLFPIGSDCVRHFHRLSLDQQLEEEEKLLRKVENLTRKA
                     QKKEKIKVNKSDFDERLLKWLWEKGVFKPNRGNQFAPERDYQLFLEVFQGGSWTKAEP
                     KKKARMEEVLEKCIKPFLLGKSDDQLYLVKLGKEKIDYEQELRIQAEKERKKRDKIAK
                     QYADNLVLAMGPAERAYQDYFGFTETLTQEERKWEKILFGKNRAERAIKAKQYQKELE
                     KDQRIASQDPIERKQKQTWLLNSYFRELPEEKARFSRLLLEYRKSGEVPFSTEYLSDH
                     LIDFFYKMKAFEFEIAPEQVRDFLKKSLQEDHRSSAQGSWIEGILLNCLKPFLGRLVI
                     "
BASE COUNT          477 a          270 c          295 g          349 t
ORIGIN      
        1 acaacctcaa aacagtgttt tgagctgact gcggctagct ttctagtttg ctctttgatt
       61 tttattgagt attagaaagg agacctcatg acacttccag ttagaaaatc cctgcacgat
      121 gcagttttac aggcttcgaa agcggatact tgggaacaag ctaccaagga atggaatgaa
      181 gtttccttga tgtttaacgg tatcggccgt agcaattgtg tctgtggaaa tgccatcaaa
      241 tacgcttatg aactctttaa cggtgtcaca ggccaacgcc tctttccaat tggtagcgac
      301 tgtgttcgtc attttcatcg attgtccctt gaccagcaat tggaagagga agaaaaactg
      361 ctcagaaagg ttgaaaatct aaccagaaaa gctcagaaaa aggaaaaaat caaggtcaat
      421 aaaagtgact ttgacgagcg acttctaaaa tggctctggg aaaaaggtgt tttcaaaccc
      481 aatcgtggca atcaatttgc gcctgagaga gactaccagc ttttcctaga agtctttcag
      541 ggaggaagtt ggaccaaggc ggaaccaaag aaaaaggctc ggatggaaga agtccttgaa
      601 aagtgtatca aaccattttt acttgggaaa tcggatgacc aactctacct tgtcaagcta
      661 ggcaaggaga aaattgacta cgaacaagaa ctccgtatcc aggcagagaa agaacgcaag
      721 aagagggata aaatcgccaa gcaatatgca gataatctgg ttctagccat gggacccgca
      781 gagcgtgctt atcaagatta ctttggcttt acagagaccc tgactcaaga agaacgcaag
      841 tgggaaaaaa ttctttttgg taaaaataga gcagaacggg ctatcaaggc caaacaatac
      901 caaaaagagc tggaaaaaga ccaacgaatt gccagtcagg atccaattga aagaaaacag
      961 aagcagactt ggctcctcaa ttcctatttt cgagagcttc ctgaagaaaa agctcgattt
     1021 tctaggctct tattagaata tcgaaaaagt ggtgaagtac ccttttcaac tgaatacctg
     1081 tcagatcatc tcatcgactt tttctataaa atgaaagcct ttgaatttga aatcgcacca
     1141 gaacaagtcc gagattttct aaaaaaaagc ctgcaggaag accatcgctc atcagcacaa
     1201 ggaagctgga tagaaggaat tctcctcaac tgcctcaaac catttttagg acgattagtg
     1261 atatgagtaa cacctaccgt gtaaatttca tggtaggtgt tattgtaccc caaaataata
     1321 taaaacaaag tatgaaagcg catctcaatt ttaagacttt tttgtaaatt aagagtataa
     1381 ttaaggtata c
//
LOCUS       DAVLVP010000168         1370 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.168, whole genome shotgun sequence.
ACCESSION   DAVLVP010000168 DAVLVP010000000
VERSION     DAVLVP010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1370
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.168"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            104..1165
                     /gene="dinB"
                     /locus_tag="VYN69_002009"
     CDS             104..1165
                     /gene="dinB"
                     /locus_tag="VYN69_002009"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000904550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEV1534214.1"
                     /translation="MLIFPLLNDLSRKIIHIDMDAFFAAVEIKDNPKLRGKPVIIGSD
                     PRQTGGRGVVSTCSYEARAFGVHSAMSSKEAYERCPQAVFISGNYEKYKAVGLQIRAI
                     FKRYTDLIEPMSIDEAYLDVTENKLGIKSAVKIARLIQKDIWQELHLTASAGISYNKF
                     LAKMASDYQKPHGLTVILPEQAEDFLKQMDISKFHGVGKKTVERLHQMGVFTGADLLE
                     VPEVTLIDRFGRLGYDLYRKARGIHNSPVKSNRIPKSIGKEKTYGKILRAEEDIKKEL
                     TLLSEKVALNLHQQEKAGKIVILKIRYEDFSTLTKRKSLAQKTQDASQISQIALQLYE
                     ELSEKERGVRLLGITMTGF"
BASE COUNT          434 a          264 c          298 g          374 t
ORIGIN      
        1 ctaggaagct agccgcagat tgctcaaaac actgttttga ggttgcagat ggaagctgac
       61 gtggtttgaa gagattttcg aagagtataa aaaggtgcta ggcatgttga tttttccttt
      121 gttaaatgat ttgtcaagaa aaatcatcca tattgacatg gatgcctttt ttgctgcggt
      181 ggaaatcaag gataatccta aactcagagg aaaacctgtc attattggaa gcgaccctcg
      241 gcaaacaggt ggacggggag tcgtttctac ctgtagctat gaggctcgag cttttggtgt
      301 ccattctgcc atgagttcca aggaagctta tgaacgttgt ccccaggccg tctttatctc
      361 aggaaattat gagaaataca aagctgtggg actccagatt cgagctattt ttaagcgcta
      421 tacagatttg attgaaccca tgagcattga cgaagcctat ttggatgtga cagaaaataa
      481 actcggtatc aagtcagcgg tcaaaattgc tcgcctcatt caaaaagata tctggcaaga
      541 actccatcta actgcttctg ccggtatttc ctacaacaag ttcttagcta aaatggcgag
      601 tgattatcaa aaaccacatg gtttgacagt gattctacct gaacaggctg aggattttct
      661 caaacaaatg gatatttcca aatttcatgg agtaggaaaa aagacagtag aacgtcttca
      721 tcaaatgggc gtttttactg gtgctgattt acttgaagtt cctgaggtga ccctaataga
      781 ccgttttggt agactaggct atgatctata tcgaaaggct cgtggcattc acaactctcc
      841 agtcaaatcc aatcgcatcc ctaaatcaat cggcaaggag aaaacctacg ggaagattct
      901 ccgtgccgag gaagatatca aaaaagagct gactcttcta tcagaaaaag tcgctctcaa
      961 tctacatcaa caagaaaaag ctggaaaaat tgtcattttg aaaatccgct acgaggactt
     1021 ttcaactctt accaaacgaa aaagtcttgc tcaaaaaaca caagatgcta gtcagataag
     1081 ccaaatagcc ctgcaactct atgaagaatt aagcgagaaa gaaagaggtg tccgcctatt
     1141 ggggattacc atgactggat tttaaagctc aatgaaaatc aaagagcaaa ctaggaggct
     1201 agccgcaggc tgctcaaaac actgttttga ggttgcagat aaagctgacg cggtttgaag
     1261 agattttcta agagtataaa accttgaaga gttgcccctt caaggttttt cttaggctct
     1321 ataatatttg tagtgggtaa atccactata gatattatgg agcctatttt
//
LOCUS       DAVLVP010000169         1318 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.169, whole genome shotgun sequence.
ACCESSION   DAVLVP010000169 DAVLVP010000000
VERSION     DAVLVP010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1318
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.169"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            67..351
                     /locus_tag="VYN69_002010"
     CDS             67..351
                     /locus_tag="VYN69_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002923859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534215.1"
                     /translation="MDWYDYMIQASKQSQFNASHWFRYLRKVIFEDYSYLTNQDVEKL
                     LDSKELTRFQKISLKYAFQEHTPTHKYVISLNKPAKLTNVQKLMEKYKHG"
     gene            344..582
                     /locus_tag="VYN69_002011"
                     /pseudo
     CDS             344..582
                     /locus_tag="VYN69_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016397100.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            678..896
                     /locus_tag="VYN69_002012"
     CDS             678..896
                     /locus_tag="VYN69_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001849851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534216.1"
                     /translation="MMSIREQDLKDIGAIIKYKNFHSPFDTFKYLKDMGFDTIDLSVL
                     LEGFSYAYGMDWLEKFFKENQDKLREFY"
BASE COUNT          475 a          211 c          216 g          416 t
ORIGIN      
        1 taagaaaaac tacatttttg aatataaact acaataatat aaactaaatt ttataggagg
       61 aagacaatgg attggtacga ttatatgata caggcatcca aacaatcaca attcaacgca
      121 agccattggt ttcgctattt gcgaaaagtt atttttgaag actattctta tttaacaaac
      181 caagatgtag aaaagttgct agactccaaa gaactaaccc gttttcaaaa aattagcttg
      241 aagtatgcct ttcaagagca tactccaact cataaatatg tgatttcatt aaataaacct
      301 gctaagttaa ccaatgttca aaaattgatg gagaaataca aacatggata aaatgaaacc
      361 ggtcttccaa gccctaaata agaaattaat tcaggaaaat ctgactttaa caattatggt
      421 tatgtcttag aatatcatgg tttacgtgcc acacaagatg ttgatgcttt ttatgaccaa
      481 aatcagaaaa tcaatgaaat tattgctcgt gtaggaaaac agtttaatct taacatccac
      541 gaagagttat ggctcaacaa tcatgttgca aagcaaatat gaacaaacaa cctcccttaa
      601 gtttatgcga atccttgtac tcatttgaga atctgaccgt tttagtagtc cctattgagt
      661 atgtgttagg aatgaagatg atgagtatca gagagcagga tttgaaagat attggtgcaa
      721 ttattaaata caaaaacttt cattcaccat tcgatacctt taaatattta aaagacatgg
      781 gatttgatac tattgacctc tctgttctat tagaaggttt tagttatgct tatggtatgg
      841 attggttaga aaaatttttt aaagaaaatc aagataaact tcgcgaattt tattaacagt
      901 atcaaacaag atgattgtca attagccagt cgtcttattt ttatgatgga atgaaataaa
      961 atcggaacaa atcacttaga gaattccagt caatttctag cactgtttga gacgtttcag
     1021 cggactatag taaaatgaaa taagaatagg acaaatcgat caggacagtc aaatcgattt
     1081 ctaagaatgt tttagaagta aaggtgtact attctacttt caatctatta tatttcagat
     1141 tcggtccact attccttccc aaaaacattg catattgcgc cgaaggaatt gctcatacta
     1201 tcccaagcac aaaaccctca gtcgtttgac cgagggttcc tttttattat tcaagaactt
     1261 gaggctctat aatatttgta gtgggtaaat cccctatgga tattatggag cctatttt
//
LOCUS       DAVLVP010000170         1239 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.170, whole genome shotgun sequence.
ACCESSION   DAVLVP010000170 DAVLVP010000000
VERSION     DAVLVP010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1239
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.170"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..266)
                     /locus_tag="VYN69_002013"
     CDS             complement(<1..266)
                     /locus_tag="VYN69_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001155321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEV1534217.1"
                     /translation="MQNHYTTKGEHLTIDSRRLIERWKKEGKSNREIASLLGKVPQTI
                     HTEIKRGTVRQCLGKGRFKEVYSADYAQQSYENNRKRSVKKSSLT"
     gene            complement(724..1122)
                     /locus_tag="VYN69_002014"
     CDS             complement(724..1122)
                     /locus_tag="VYN69_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEV1534218.1"
                     /translation="MENKESHKSQEIGNLIRVYNRSKREEAESEPLNLYVEDEKGNLL
                     AGLIAETFGNWLEIEYLFVKEELRGQGIGSKLLQQAESEAKNRNCCFAFVNTYQFQAP
                     DFYQKHGYKEVFSLQDYLYIRQRYYYQKNL"
BASE COUNT          356 a          246 c          194 g          443 t
ORIGIN      
        1 gtcaagcttg atttcttgac cgagcgcttg cgattgtttt cataagactg ttgagcatag
       61 tcggcagaat aaacctcttt gaagcgccct tttccaagac attgtcggac tgtcccacgc
      121 ttgatttcag tgtggatagt ttgaggaact tttccaagca gagaggcaat ttctctattt
      181 gatttccctt cttttttcca tctttcgatt aagcgacggc tatcgattgt caaatgttcg
      241 ccttttgtag tataatggtt ttgcatctct gtgcctttct tgtgtttgtg gttgaacaac
      301 aagtataaca cagaggtgtt ttcttatgcc tacaagagct ttcattattt ccattttctt
      361 tcggatttca ctctattctg aaaaacttgt gtatatttac tgaagctagc aagttttacc
      421 tgtaaattta atgaaagcaa cacaaaatcc gagaggggaa tctcggatta atgtcaattt
      481 aaaaagatag agagttttta gtttaaataa attgttttaa aatatcaaca acatcacttc
      541 ttttcttaac ctgataagcc ttgattccta attttggagc tacgattata ttgtcctcaa
      601 tatcgtctag aaagacacaa tttctaggtt ataactggta tttatcgata gttactcata
      661 tacatcagtc cacctccata cttatgtgcg agcctctctt tgtattatac ctccatactc
      721 accttacaga ttcttttggt aataatatct ttgcctaatg tagagatagt cttgcaaaga
      781 aaaaacttcc ttgtagccat gtttctgata aaagtccggt gcctggaact gataagtatt
      841 gacaaaggca aaacaacaat ttcgattctt agcttcactt tctgcctgtt gcaatagttt
      901 tgaaccgatt ccttgccctc gcagttcctc ttttacaaac aaatactcga tttctagcca
      961 atttccaaaa gtctctgcta tcaaacccgc aaggagattg cccttttcat cttcgacata
     1021 aagattaagt ggctcacttt cagcctcttc cctttttgaa cggttataaa cacgaatcag
     1081 attccctatt tcttgcgatt tatgtgattc cttattttcc aatctaaagt ataatgaaat
     1141 gaaataaaac atgcgcaaat cgattaagga atttaatcta atttctaata atgtcttaga
     1201 aatcaaagtt tactatttta acttcaatgc actatacat
//
LOCUS       DAVLVP010000171         1153 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.171, whole genome shotgun sequence.
ACCESSION   DAVLVP010000171 DAVLVP010000000
VERSION     DAVLVP010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1153)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1153)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1153
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.171"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            51..779
                     /locus_tag="VYN69_002015"
     CDS             51..779
                     /locus_tag="VYN69_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEV1534219.1"
                     /translation="MSTKYLFIYNEIREKILCNKYTMNEQLPDEMTLAKQFACSRMTI
                     KKALDLLVSEGLIFRKRGQGTFVLSRGSSKRKLIVPERDIRGLTKISEDAHSTIDSRI
                     IHFKLEFANEFLAEKLQVALQSPVYNIYRLRIIDGKPYVLEQTYMSTDVIPGITEDIL
                     QKSIYNYIEGKLGLHIASATKILRASSSSENEQHYLQLLPTEPVFEVEQVAYLDNGTP
                     FEYSISRHRYDLFEFNSFALRHSS"
BASE COUNT          385 a          178 c          201 g          389 t
ORIGIN      
        1 ttgaactttg ttatactact attagaatat tatagagaga accagattaa atgagtacaa
       61 aatatttatt tatttacaat gagattcgtg aaaagattct ttgtaataaa tataccatga
      121 acgaacaatt gcctgatgaa atgacattag ctaaacagtt tgcctgtagt cgaatgacga
      181 tcaaaaaagc tttagacttg ttagtttctg agggcttaat ttttagaaaa cgtgggcagg
      241 gaacctttgt tctctctcgt ggcagctcaa aaagaaaatt aatcgttcca gaaagagata
      301 tccggggact gacaaaaata tctgaagatg ctcattctac aattgactcg aggattattc
      361 acttcaaatt agaatttgca aatgaatttt tagcagaaaa actacaggtc gctttgcaga
      421 gtccagttta taatatttac cgcctgcgta ttattgacgg taaaccttat gttctggaac
      481 aaacttatat gagtaccgat gttattccag gtattactga agatatttta caaaaatcga
      541 tttacaatta cattgaagga aagttaggat tgcatattgc cagtgctaca aaaatcttac
      601 gagcttcttc tagttcagaa aatgagcaac attacttgca gctccttcca acggaaccgg
      661 tatttgaagt agaacaagtg gcttatttgg ataacggaac tccgtttgag tactcgatta
      721 gtcgtcatcg ctatgattta tttgaattta attcttttgc attacgacat tcctcctagg
      781 agaaaatgtg aaaatgaagc caatctttta caggctctag tttaagaaaa atttaaaaca
      841 gggcaagaag gtcccatcta tgcttaaatg gtttctcttt tctaaataag atgactttaa
      901 aagagtgatc gttttatcca tcatgttgaa aaatatcttc gtatagctta tagagtaggt
      961 actgaaattg ttcacctgat ctacttctta tagttattta gttttaaata gtgtttcaaa
     1021 cattcttaca ctgacgagaa gtttttgagt cttttcttgt aacacatata gtatattgag
     1081 gttagaatag tagactgtga cttctaaaaa attgctagaa atgaatttca atctcccaat
     1141 ttatttgttc ata
//
LOCUS       DAVLVP010000172         1148 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.172, whole genome shotgun sequence.
ACCESSION   DAVLVP010000172 DAVLVP010000000
VERSION     DAVLVP010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1148)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1148)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1148
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.172"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            50..781
                     /locus_tag="VYN69_002016"
     CDS             50..781
                     /locus_tag="VYN69_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1534220.1"
                     /translation="MVNNVAVKVSNLSKEFLLGQDKTVSILKDVSLSVNYGEFVSILG
                     VSGSGKSTLLSCLSSLSEPTSGEVVINGVNPYTLKEGKLAKFRRQDIAIIFQNYNLIP
                     ALPVLENVTLPLRLSGKSVDSNKVKKMLDSLNFKAELSSLVATLSGGEQQKVAITRAI
                     IADSKIIFADEPTGALDSVSRKLIFETLRNLASQGKCVLMVTHDIELASKTDRALILK
                     DGKISRQIIKPSADELYQALESSKD"
     gene            799..>1148
                     /locus_tag="VYN69_002017"
     CDS             799..>1148
                     /locus_tag="VYN69_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000915989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-carotene 15,15'-monooxygenase"
                     /protein_id="HEV1534221.1"
                     /translation="MLKLIIYQFQYSKRQWLGTIPLLFVSSLIVGTSLFGIASSIKTA
                     NINASQLFQMLIIFGGTTLFFLISNNIRLLIDIFKKDYQLWAILGASRTQLSLLVSGQ
                     FYLMAVIVSSIGTIL"
BASE COUNT          395 a          171 c          200 g          382 t
ORIGIN      
        1 atattgtata attaaatcaa agatgacact ggaatcagga ggatttatta tggttaataa
       61 tgtagctgtt aaagtctcaa acctttcaaa agaatttttg ttagggcaag acaaaactgt
      121 ctctattttg aaagatgttt ctttatctgt caattatggg gaatttgtat caatacttgg
      181 tgtcagtggt tctggaaagt ctactttatt aagttgctta tcaagtttat ctgaaccaac
      241 aagtggcgaa gttgttatca atggtgtaaa tccatatacc ttaaaagaag ggaagcttgc
      301 taaatttaga agacaagata ttgcaataat ttttcaaaac tataatctga tacccgctct
      361 acctgtacta gaaaatgtta cacttcctct gagactctca ggaaagagtg ttgatagcaa
      421 taaagtaaaa aaaatgttgg atagtttgaa ttttaaagca gaactatcat cattagttgc
      481 taccttatca ggtggagaac agcaaaaagt ggctattact cgtgcaataa tagctgatag
      541 taaaattatt tttgctgatg agccaacagg agctctggac agcgtctcaa gaaaacttat
      601 atttgaaaca cttcgtaatt tagcaagtca aggaaaatgt gttcttatgg tcactcatga
      661 tattgagttg gcttcaaaaa ctgacagagc actcatttta aaagacggaa aaatatctcg
      721 acaaattatt aaaccttcag ctgatgagct ctaccaagca cttgaaagta gtaaagatta
      781 atttgaggag gatttattat gctaaaatta atcatttacc aatttcagta ctcaaaaaga
      841 caatggttag gaacaatacc attactattt gtatctagcc taattgttgg aacatcttta
      901 tttggtattg ctagttcaat caagactgct aacattaacg cttcacaact ttttcaaatg
      961 ttaataattt ttggcggaac tactctattt ttccttattt caaataatat aagactgcta
     1021 atagatatct ttaaaaagga ttaccaatta tgggctatct taggcgcaag cagaactcag
     1081 ctatctttat tagtatctgg acagttttat ttaatggcag tgattgttag tagtattggt
     1141 acaatact
//
LOCUS       DAVLVP010000173         1123 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.173, whole genome shotgun sequence.
ACCESSION   DAVLVP010000173 DAVLVP010000000
VERSION     DAVLVP010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.173"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            373..>1123
                     /locus_tag="VYN69_002018"
     CDS             373..>1123
                     /locus_tag="VYN69_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019213953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobA/MobL family protein"
                     /protein_id="HEV1534222.1"
                     /translation="MADSFHFSVNIISRGKGKSAVASAAYISGEKIKNEWDGVTHDYT
                     RKEKILVKNIILPDHIPKEFNDRSILWNKVEMAEKNSNAQLARQFIIGLPKELSLSEN
                     KNLVERYIKENLTSQGMIVDYAIHDESQDKNGNIHCHIMTIMRPINEKGEFLAKSKKE
                     YILDEKGEKVLNKNGKPKTRKVELTTWNDTGNVEQWRENFSDLCNKYLERAGAEKRVD
                     HRSFKRQNSDYLPTIHLGSAASAMERKGIETD"
BASE COUNT          507 a          159 c          201 g          256 t
ORIGIN      
        1 gaaattttag aagaaagaat agatgataat gtagaaaaat catctaaaga tgaaataaca
       61 taattgtccc ttggttacaa gggcgcaatt atacacccta aagggtgctt gcgtcctgcg
      121 gagtcaaacc cttgcaggga gcaacaacca ttcgcttttt aaaagtcaag ggtaaataaa
      181 ctcgctaagg ctcgcccttg acttttaaaa tttgaaatga aagtaacaat taagccgaca
      241 tactgaaaca acaaaaatta attcagtagt cggctttttt tattctatca tttcaaaacg
      301 ctcattcaca aagaggatat aaaaaaacta ttaagcctcc acctaaaaca acaaagaaag
      361 gaagtgataa aaatggcaga cagttttcat ttttcagtaa acataatatc aagaggaaaa
      421 ggcaaaagtg cagtagcaag tgcagcatat ataagtggag aaaaaataaa aaatgaatgg
      481 gacggagtaa cccatgacta cacaagaaaa gaaaaaatat tagtaaaaaa tataatattg
      541 cctgatcata taccaaaaga atttaatgat aggtctatct tatggaataa agtagaaatg
      601 gcagaaaaaa attctaatgc acaactagca agacaattca taataggact accaaaagag
      661 ttatctttaa gtgaaaataa aaacctggtt gaaagatata taaaagaaaa tttaacatca
      721 caaggaatga tagtagatta tgcaattcat gatgagagtc aagataaaaa tggaaatatc
      781 cattgtcata taatgaccat aatgcgacca ataaacgaaa aaggagagtt cttagccaag
      841 tcaaaaaaag aatatatcct agatgaaaaa ggagaaaaag tattaaacaa aaatggaaaa
      901 ccaaagacaa gaaaagtaga actaacaacc tggaacgata caggcaatgt agaacaatgg
      961 agagaaaatt tttcagatct ttgtaacaaa tatttagaaa gagcaggagc agaaaaaaga
     1021 gtagaccata ggagttttaa aagacaaaat tcagattatc taccgacaat ccatttagga
     1081 tcagcagcaa gtgcaatgga aagaaaggga atagaaactg aca
//
LOCUS       DAVLVP010000174         1110 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.174, whole genome shotgun sequence.
ACCESSION   DAVLVP010000174 DAVLVP010000000
VERSION     DAVLVP010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1110
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.174"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(233..544)
                     /locus_tag="VYN69_002019"
     CDS             complement(233..544)
                     /locus_tag="VYN69_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534223.1"
                     /translation="MIEAQGFLVDKQTRCIHYHSKLDIIALQCYDCKKYYACYRCHDS
                     LENHPFEPYPLSLIQDKPILCGVCLKLLTYKQYKESLSCSFCFSRFNPGCQNHKERYF
                     K"
     gene            complement(559..>1110)
                     /locus_tag="VYN69_002020"
     CDS             complement(559..>1110)
                     /locus_tag="VYN69_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase C-terminal
                     domain-containing protein"
                     /protein_id="HEV1534224.1"
                     /translation="GVDSLKIEGRMKSIHYVSTVTNCYKAAVDAYLESPEKFEAIKQD
                     LVDEMWKVAQRELATGFYYGIPSENEQLFGARRKIPEYKFVAEVVSYDDAAQTATIRQ
                     RNVINEGDQVEFYGPGFRHFETYIEDLHDAKGNKIDRAPNPMELLTIKVPQPVQSGDM
                     VRALKEGLINFYKEDGTSVTVRA"
BASE COUNT          336 a          220 c          192 g          362 t
ORIGIN      
        1 agacgcaatt ctaatctttt agactttgca taataactgt taaacagttg aaaattaacc
       61 cagttttaac aagtagtcaa aataccagat aaaggaaaaa agtctttaaa atcttaaaaa
      121 cgcatagtat caggtattga acaatcttga tattatgcgt tttattgtgg gaagatttac
      181 ttcattttct cttggaattg agttttctcc tacttcaaaa ctagatgatt tgctatttaa
      241 aatagcgttc cttatgattt tggcaacctg gattaaagcg agaaaaacaa aaggagcaac
      301 ttaaactttc tttatattgc ttatatgtta gtagttttag acaaacacca cataaaatag
      361 gcttatcctg tatcaaagat aagggatacg gctcaaaagg gtgattttct aatgaatcat
      421 gacaccgata acaagcatag tactttttac aatcatagca ttgtaaagca ataatatcca
      481 acttgctatg gtaatgaatg catcttgttt gcttatccac taagaaaccc tgtgcctcta
      541 tcatttcctt ttcctttctt aagctcgaac tgtgacgctg gttccatctt ccttataaaa
      601 attgatgagt ccttctttta atgcacgaac catgtctcct gattgaacgg gttgtgggac
      661 tttaatagtc aatagttcca ttggatttgg agcgcggtcg attttattgc ctttggcatc
      721 atgcaaatct tcaatatagg tttcaaaatg acggaaacct ggaccataaa actcaacttg
      781 gtccccttcg ttaatgacgt ttcgttgacg aatggttgct gtttgtgccg catcatcata
      841 agaaaccact tcagcgacaa acttgtactc aggaatttta cggcgagcac caaacaactg
      901 ctcattttca gatggtatac cgtagtagaa acctgttgcc aattcacgtt gggcaacctt
      961 ccacatctcg tccaccaagt cttgtttgat agcttcaaac ttttcaggac tttcaagata
     1021 ggcatccaca gccgccttgt agcagttggt tactgttgat acgtagtgaa tagacttcat
     1081 acgtccttcg atttttagac tgtccacacc
//
LOCUS       DAVLVP010000175         1024 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.175, whole genome shotgun sequence.
ACCESSION   DAVLVP010000175 DAVLVP010000000
VERSION     DAVLVP010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1024
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.175"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            325..507
                     /locus_tag="VYN69_002021"
                     /pseudo
     CDS             325..507
                     /locus_tag="VYN69_002021"
                     /inference="COORDINATES: protein motif:HMM:NF024172.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          394 a          186 c          139 g          305 t
ORIGIN      
        1 tttagcgcca catagtactg aactctctcc aaaaacggtt attcctcttt gaatagggcg
       61 ttatcacaag aaaagcatct ccacgtttca acttcatatg gctcaaaaac aatcaattga
      121 tgctaaaacc tgtacctaga tgtttcggtt cataaaacca tgaaactgta aaagtggatg
      181 aaattgatag cgatagtcaa atcaagaggc atcataactc taaaaagtca caatatataa
      241 gttcatcctc ggaaaaatat cattctaatt gttgaaatgc ctacatgaaa agaaacgtca
      301 aatgctcatg aaacaacgaa tacaggtatc aaaactatga caaaacaagt ccctaaattt
      361 actaaagaca ctgctcaact ttacacctgt aaatggttgt tgtataataa agttacaaag
      421 atgtacgacc acactgttgt aaatcatagt gttcgcgaat atattactga tagcatttct
      481 acaaatacaa ttgaagagac tggatagact ttaaacgaaa tatgtcagtg ttttggcatt
      541 cctagccttc atatcattta aagaattcta tagacaaaat ttttcccaat acagacactc
      601 gtaacaactg cttcattttt ctaccaacat atttaggaac aggataagat acaagaggat
      661 taatccatag ctcagttcta taccaatcta agacaaataa gctaaaaaaa cgattgataa
      721 taagcaaata gattccaaat tttctctatc tgctcatttt aataaacaat actagtgtaa
      781 ctatccttcc agtcagaagc ttgtcaaatc acaccgaaaa ttcttctaaa atttatctcg
      841 ttaggcaatc aagcaaaaac tcgacgatag tacaaacatt atcatacaga attgacttcc
      901 taaattatat actttagtaa ggttttcgga taagaaaaaa ggttcatttt acatttctaa
      961 acattctttt ctaagatgaa aaacagaatt tttcgattgt aatttaaagc aacaagaaga
     1021 tttt
//
LOCUS       DAVLVP010000176         1017 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.176, whole genome shotgun sequence.
ACCESSION   DAVLVP010000176 DAVLVP010000000
VERSION     DAVLVP010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1017)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1017)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1017
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.176"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            1..1017
                     /locus_tag="VYN69_002022"
                     /pseudo
     CDS             1..1017
                     /locus_tag="VYN69_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611512.1"
                     /note="frameshifted; internal stop; incomplete; missing
                     N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          383 a          180 c          206 g          248 t
ORIGIN      
        1 caagaaatga ttcataaagt ctgacatgaa ggctggacta aagatagagt ttctcttgaa
       61 tacggtctcc caagtcgtac gatacttctt aactggctag cacaatacag gaaaaacggg
      121 tatactattg ttgagaaaac aagagggaga gtacctaaaa tgggacgtaa gccaaaaacg
      181 agacctgaag agaggacaga attagaacgt cttcaagcag aaaatgagta cctgagagcg
      241 gagaatgcca tcctaaaaaa gttaagagaa ctccgattga aggaggaaaa agagaaagaa
      301 gaaagacaga aattgttcaa gaattaatga ctgagttttc gttagatctt cttctaaaag
      361 ccattaaact agctcgttgg acctactact atcacttgaa acagctagat aaaccagata
      421 aggaccaaga gcttaaagct gaaattcaat ccatctttat cgaacacaag ggaaattatg
      481 cttatcgtcg gatttattta gaactaagaa atcgtggtta tctggtaaat cataaaagag
      541 ttcaaggctt gataaaagta ctcaatttac aagctaaaat gcgacaaaaa cgaaaatatt
      601 cttctcataa aggagacgtt ggtaagaaag cagagaatct cattcaaggc caatttgaag
      661 gctctaaaac aatggaaaag tgctacacag atgtgacaga atttgccatt ccagcaagta
      721 ctcaaaagct ttacttatta ccagttttag atggctttaa tagcgaaatt atcgcctata
      781 atctttcaac ttcacccaac ttagaacaag tacaaacaat gttggaacag gcattcacag
      841 agaagcacta cgagaatacg attctccata gtgaccaagg ctggcaatat caacacgatt
      901 cttatcatcg gttcctagag agtaagggaa ttcaagcatc tatgtcacgc aagggaaaca
      961 gccaagacaa cggtatgatg gagtccttct ttgggattct gaaatcggaa atgtttt
//
LOCUS       DAVLVP010000177         1014 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.177, whole genome shotgun sequence.
ACCESSION   DAVLVP010000177 DAVLVP010000000
VERSION     DAVLVP010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1014
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.177"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            30..>1014
                     /locus_tag="VYN69_002023"
     CDS             30..>1014
                     /locus_tag="VYN69_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001244688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEV1534225.1"
                     /translation="MRNKKQINLGIIFVICLGLLITIFLSLKLGTKEINIRDFLAAFG
                     MGNTNDDFIKSIIYKRIPRTIFAILAGSSLAISGVLMQSVTRNPIADPGILGINTGAS
                     LSVVIGLSFLGISSSISHISFAIIGGLVSAIFVYAIAVSGKAGLTPIKLALSGTCVSM
                     ALSSFVSFLILPNNNVLDKFRFWQIGSLGAATLSSISTLLPFIILGHLIAIFISSDLN
                     ALAMGDEMAVGLGVNVNRIRSLAIIASVLLCSSITAIGGPIGFVGLIVPHFCGLFISK
                     DIRTMTISSSFIGAELLLICDIIGRMLGKPGEIEVGIITAIIGGPVLIYVTM"
BASE COUNT          315 a          149 c          188 g          362 t
ORIGIN      
        1 tctaaatctt ttaggaaatg catgcaaaaa tgcgaaataa aaaacaaata aacctaggca
       61 taatttttgt aatctgccta ggtcttctta ttacaatatt tttgtcatta aagcttggaa
      121 caaaagaaat taatatcaga gattttttag cagcttttgg aatgggtaat acaaatgatg
      181 attttattaa atcaattata tataaaagaa tacctagaac tatttttgca attttagcag
      241 gttctagtct tgccataagc ggtgtattga tgcaatcagt tactagaaac ccaatagctg
      301 atccaggtat actcggtata aacacaggag caagtcttag tgtagtaatt ggtctttctt
      361 ttttaggaat ttcatcaagc ataagccata taagttttgc aatcattggt ggcttagtaa
      421 gtgcaatttt tgtatacgcg attgctgtaa gcggaaaagc aggccttacc cctataaaac
      481 ttgccttatc aggaacttgt gttagtatgg ctttaagcag ttttgtaagt tttttaattt
      541 taccgaataa taacgtctta gacaaattta gattttggca aataggtagc cttggagcag
      601 ctacattatc ttctatatct acactactac cttttataat tttaggtcac ttgatagcta
      661 tatttatttc atcagattta aacgctttag ctatgggtga tgaaatggct gttggtcttg
      721 gagttaatgt taataggata agatcacttg caataattgc aagtgtgctt ttatgttcaa
      781 gtattactgc aattggtgga cctattggct tcgtaggtct tatagttcct cacttttgtg
      841 gcttatttat aagcaaagat atacgcacaa tgaccatttc ttcatctttt ataggtgcag
      901 agctcttgct tatatgtgat ataatcggcc gtatgttagg taaaccaggt gaaattgaag
      961 tagggataat tactgcaata atcgggggtc cagtacttat ttatgtaact atga
//
LOCUS       DAVLVP010000178          976 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.178, whole genome shotgun sequence.
ACCESSION   DAVLVP010000178 DAVLVP010000000
VERSION     DAVLVP010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 976)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 976)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..976
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.178"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..400)
                     /locus_tag="VYN69_002024"
     CDS             complement(<1..400)
                     /locus_tag="VYN69_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534226.1"
                     /translation="MIRKLIVFLLMLPLFVLWLELHVLVNNLLLNLESPLDFVISMSL
                     AFCSLILSKIVLDILYALKDLYKKEGLIKIFPFIFIRRKKVNVRFSPYFSFHRKSLSP
                     DDLRSRIIWSFILEIAIILVFILKIPFAIIM"
     gene            complement(914..976)
                     /locus_tag="VYN69_002025"
                     /pseudo
     CDS             complement(914..976)
                     /locus_tag="VYN69_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424421.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
BASE COUNT          369 a          187 c          127 g          293 t
ORIGIN      
        1 gcatgatgat tgcaaaagga atttttaaaa taaatactag gataatagca atttcaagaa
       61 taaaactcca tataatccta gatcttaaat catcaggcga caaactttta cgatggaatg
      121 aaaaataggg cgagaatctc acattcactt ttttccttct tataaaaata aagggaaaaa
      181 tcttaataag accttctttc ttgtataaat ctttcaatgc gtatagtatg tctaaaacta
      241 tttttgataa aatcaaacta cagaaagcaa gactcatact aatcacaaaa tcaaggggag
      301 attctagatt caatagtaag ttattcacta ggacatgcaa ctctagccac aagacaaaaa
      361 gagggagcat tagtaagaaa acaattagtt tacgtatcat aattttacag tgtccttatc
      421 attttcttaa ttttggataa atcatcattg ctatgattaa aatcaaaaaa acgacattaa
      481 tccaaataat caactttttt atttcttcgc taacaaaatc ataggctaaa aaagttatgg
      541 atgatataaa tagacataca aaaaaaataa taacaaaaat tttttccatt ttaataccca
      601 tatctagtta ccacaaggcg tttaaatttg ggtctctcct atttacatca ttattggccc
      661 agattgttgt aatagcatcg ccaattgaca tccaagctgc aaatccatta atagcagaca
      721 gtgcaaatcc tgccactcta tttgcaactc gtaccccaac atatcttgca gctgctccag
      781 caattgcgct tgtaactcgc cgcaaaccat aattacgaat ataatttcta actagactga
      841 ttgctcctcc acctaaaaca ccattaaaag ctatattaat ggctgttgca acaggccatc
      901 tcggaataga aaatccccca tcaagataca tcatctcttc ttgctcaaga acaacataat
      961 tatttggtaa tacgag
//
LOCUS       DAVLVP010000179          961 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.179, whole genome shotgun sequence.
ACCESSION   DAVLVP010000179 DAVLVP010000000
VERSION     DAVLVP010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..961
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.179"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(112..465)
                     /locus_tag="VYN69_002026"
     CDS             complement(112..465)
                     /locus_tag="VYN69_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000424445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HEV1534227.1"
                     /translation="MELVLPNNYVVIDEEVMMYLDGGAYLSKRACQGICAALAMSSGT
                     FIALAGAAVLTKKLINYIKVGGLGGWLIGAAAGVLATAAGKIAYCIGYGVLNRGCDIN
                     GNPYPWDGFISATVR"
     gene            complement(521..>961)
                     /locus_tag="VYN69_002027"
     CDS             complement(521..>961)
                     /locus_tag="VYN69_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000619809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534228.1"
                     /translation="FLFNQNKWQKEDSFESNLHKILMDKIQKSELSYSDLMSLLLYDA
                     MNQSTFLTDSELFEDILKKIEDSHNILLCTGLVELLLYEISISNNNNWQEKVDKIRIQ
                     LIRINQLDFFYYTSWLRQNFDFCMNREYHKMKSRKLLLSNKKIV"
BASE COUNT          318 a          192 c          121 g          330 t
ORIGIN      
        1 ctaaaaaaat aagcaaaaaa tactccgtat catttataat cgccctgaat gataaaaata
       61 tatacaccca acacacgatg atgtgtattt ttcgaatctt atctacatta ctcatcttac
      121 tgttgcagat atgaatccat cccaaggata agggttcccg ttaatatcac aacctctatt
      181 aagaacacca tatccaatac agtaagctat tttccctgct gctgtagcca atacacctgc
      241 tgctgcacca ataagccagc ctccaagacc tccaacctta atatagttta ttagtttttt
      301 ggttaaaact gcagctccag ctaatgctat aaaagttcct gaactcatag ctaaagctgc
      361 gcaaattcct tgacaagcac gcttgcttaa ataagctccc ccatcaagat acatcattac
      421 ttcttcatca atcacaacat aattatttgg taatacaagt tccatttgtt ttctcctcct
      481 tagttgaaat tgtttcattt gttttacaaa taggacgaac ttaaactatc tttttattac
      541 tcagtaataa tttccgagat ttcattttat gatactctct attcatacag aaatcaaaat
      601 tttgccttaa ccaacttgta taatagaaaa aatctaattg atttattctg atgagctgta
      661 ttctaatttt atcaaccttt tcttgccagt tgttattgtt actaatagaa atctcataca
      721 gcagtaactc gacaagacct gtacacagaa ggatgttatg agaatcttcg atttttttca
      781 aaatatcctc aaaaagttca ctatccgtta aaaaggtgga ttggttcatc gcatcataga
      841 gaagaaggga cattaagtca gaatagctta attctgattt ttgtattttg tccatcaata
      901 tcttatgcaa attactttca aatgaatctt ctttttgcca tttgttttga ttaaaaagaa
      961 a
//
LOCUS       DAVLVP010000180          959 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.180, whole genome shotgun sequence.
ACCESSION   DAVLVP010000180 DAVLVP010000000
VERSION     DAVLVP010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..959
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.180"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            247..>959
                     /locus_tag="VYN69_002028"
     CDS             247..>959
                     /locus_tag="VYN69_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000169115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEV1534229.1"
                     /translation="MTKTLKRPEVLSPAGTLEKLKVAVQYGADAVFIGGQAYGLRSRA
                     GNFTFEQMEEGVQFAAKYGAKVYVAANMVMHEGNEAGAGEWFRKLRDIGIAAVIVSDP
                     ALIMIAATEAPGLEIHLSTQASATNYETLEFWKELGLTRVVLAREVSMEELAEIRKRT
                     DVEIEAFVHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYDLYDMPFGKERKSL
                     QGEIPEEFSMSAVDMSMID"
BASE COUNT          269 a          171 c          224 g          295 t
ORIGIN      
        1 gtagtcatct aaggactaat ttatgtattt ttacgagtca gtatctttgg atactggttt
       61 ttacttttct agactttttg actacttgtt aaaactggga taattttcga ctgtttaaca
      121 gttattatgc aaagtctaaa agattagaat tgtcaaaaca atccgtctag gcttgatttt
      181 atcctttatt tactataaaa tgagaaggaa aaatgtcaaa cttttatatt gcaaatagga
      241 gaaattatga caaaaacatt aaaacgtcct gaggttttat cacctgcagg gactttagag
      301 aagctaaagg tagctgttca gtatggagca gatgctgtct ttatcggtgg tcaggcctat
      361 ggtcttcgta gccgtgccgg aaactttact ttcgaacaga tggaagaagg cgtgcagttt
      421 gcggccaagt atggtgccaa ggtctatgta gcggctaata tggttatgca cgaaggaaat
      481 gaagctggtg ctggtgagtg gttccgtaaa ctgcgtgata tcgggattgc agcagttatc
      541 gtatctgacc cagccttgat tatgattgca gctactgaag caccaggcct tgaaatccac
      601 ctttctaccc aagccagtgc cactaactat gaaacccttg agttctggaa agagctaggc
      661 ttgactcgtg tcgttttagc gcgtgaggtt tcaatggaag aattagctga aatccgcaaa
      721 cgtacagatg ttgaaattga agcctttgtc catggagcta tgtgtatttc atactctgga
      781 cgttgtactc tttcaaacca catgagtatg cgtgatgcca accgtggtgg atgttctcag
      841 tcatgccgtt ggaaatacga cctttacgat atgccatttg ggaaagaacg taagagtttg
      901 cagggtgaga ttccagaaga attttcaatg tcagccgttg acatgtctat gattgacca
//
LOCUS       DAVLVP010000181          918 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.181, whole genome shotgun sequence.
ACCESSION   DAVLVP010000181 DAVLVP010000000
VERSION     DAVLVP010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..918
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.181"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..675
                     /locus_tag="VYN69_002029"
     CDS             <1..675
                     /locus_tag="VYN69_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEV1534230.1"
                     /translation="DKRIKRLRRISGGDTYTIIYLKLLLLSLKDEGKLYYDGVESDFI
                     KELALTIDETDDDVMVTVNYLINQGLLEVVTENDEYYLTEIPNLIGSETAWAEKKRRY
                     RQNKQRTLSLMSPTHVRQEIEIEKDIEIDKEREGEIDKKSTPIFYGEFKNVRLSKEEY
                     KNLKEKLNSHTDIMINKLSRYMESSGKTYQNHYVTILKWYEEDKDKLRQKGLNKKMNY
                     DVGESL"
BASE COUNT          412 a          117 c          171 g          218 t
ORIGIN      
        1 gacaaaagga taaaaaggtt aaggagaata tctggaggag atacctatac gataatctac
       61 ctaaaacttc tactactaag cctaaaagat gagggcaaac tctattatga cggagtagaa
      121 agtgatttta taaaagagtt agcactaacc atagatgaaa cagacgatga tgtaatggta
      181 acagtaaatt atttaatcaa tcaaggctta ttagaagttg taacagaaaa tgatgaatac
      241 tacctaactg aaataccaaa cttaatcgga tcagaaacag cctgggcaga gaaaaaaaga
      301 agatacagac aaaataaaca gaggacattg tccttaatga gtccaaccca tgtccgacaa
      361 gagatagaga tagagaaaga tatagagata gataaagaga gagagggaga gatagataaa
      421 aagtccaccc ccatttttta tggagaattc aaaaatgtaa ggttaagcaa agaagaatat
      481 aaaaatctaa aagaaaaatt aaactcacac acagatataa tgataaataa actatccaga
      541 tacatggaaa gcagtggtaa gacctatcaa aaccactatg tgacaatctt aaaatggtat
      601 gaagaagata aagacaaact aagacagaaa ggtttaaata aaaaaatgaa ttatgatgta
      661 ggagaatctt tatagataaa aagaggtgga aaggcactat taaagacttt aagtttaaaa
      721 aggtatcaaa gtatgccaga gataaaaaaa tataaatatg acactttaaa ggcgattaga
      781 aaattaaaga aatgctagaa gacttttaga tgacagttac caagagggtc taattttcac
      841 tgctacttat gcagtaggaa ttggaccttt ttatataagc aaattctatt tcaaaattgg
      901 ttaccaagaa agacttct
//
LOCUS       DAVLVP010000182          867 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.182, whole genome shotgun sequence.
ACCESSION   DAVLVP010000182 DAVLVP010000000
VERSION     DAVLVP010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 867)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..867
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.182"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            16..800
                     /locus_tag="VYN69_002030"
                     /pseudo
     CDS             16..800
                     /locus_tag="VYN69_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000436304.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          297 a          184 c          137 g          249 t
ORIGIN      
        1 gtataaaaaa ttcaaactag aattcttctt agaaaaggcc gttttaagtg ctatcactgt
       61 tcaaaaatga gggtcgctga aacttctatc gtcaagaaga atcatcaaat tccttgtatc
      121 atcaatcaaa agattgctca gaagctaatt aaaaagactt ctatgaccga cattgatcat
      181 cagttgtcta tttcaacttc aactgtcatt cgcaagatca atgattttca ctttgagcat
      241 gatttttcgc gtcttcctga gattatgtcc taggacgttg aaacagtctg gggagtgact
      301 gtttcaatcg ggagatagag atgagcttta ttgctcagga ctttgataag ctcaatatca
      361 taactgttct tgagagcaga acacaagcca tcatccgaaa tcccatgaat acaaggctat
      421 caagcgatac tgaaagctca ttcaacaaga tagtcagaaa ctgagtgata aacgatttta
      481 tcgccctact tttcgtatgc acttgactaa taaagaaatt ctagataaac ttttgagcta
      541 ttccgaagac ttgaaacacc actataatct ctatcagctc ttgcttttcc accctgttta
      601 aaaccttcct caaagataaa gagaaaatcg tcaacgccct tcaattacct tattccaacg
      661 ccaaactgga agccaccaat aatctcatca aacttatcaa gcacaatgcc tttggtttta
      721 ggaactttga aaacttcaaa aaagaaagga cgaaatttgt cctttctaga tcttcgcttt
      781 cttcaaccca ctacagttga caaagaaccg aaaaatgtct cgcctctaat atagtcaatt
      841 gaaacaagaa caagagaaaa gagcctc
//
LOCUS       DAVLVP010000183          864 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.183, whole genome shotgun sequence.
ACCESSION   DAVLVP010000183 DAVLVP010000000
VERSION     DAVLVP010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..864
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.183"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(716..>864)
                     /locus_tag="VYN69_002031"
     CDS             complement(716..>864)
                     /locus_tag="VYN69_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001061789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HEV1534231.1"
                     /translation="SLSSAKEEYINRVLNVLDKEYGGILRYINTELGISSQEIEELKD
                     RYLF"
BASE COUNT          282 a          154 c          107 g          321 t
ORIGIN      
        1 tataatttta atatcattta cctacttcca agatgctgaa aaatactaag tcacactatt
       61 atgatgttat tatttccatt tttattttaa cagatgagtc aattctttca actaccccct
      121 cccttataac tatattcatg aagtattctt gataataaac ctcatttaac ttctcaaagt
      181 agtcagcttc tgcaactgct attatctcat aacagattga tttggtttca ataaatggaa
      241 gctagaagcc ttgtcagcta ccctgcacag ttggtgtttt ttcatttttc tcttcaaaaa
      301 aattggtcgg aagttataat aaaaaggtga tgtgtttgtg atgtgtttgt ggtgagttga
      361 attaagagac aaactcactt tgtcttaact tttaaattgc ctaacaaaaa tacggagatt
      421 attctctaag ctttaactgc aacattatgt ctctacaacc aacgataagt ccagaaatct
      481 acccaaataa ccaaacaacc tacatttgtt gaactttgat gacaagtagg aaattttaca
      541 tttgataaac actattttgt acgcattttg cacataattt aacgtgcttt ccatcaattt
      601 agttaaggac ttacatagaa aacaatccgt ttttgcttat gttaaattag tagaattaga
      661 caaaaacaaa accgccgttc gcactgttat atattcattt ttagtactta ctattctaaa
      721 aaagatatct atctttcaat tcctcaattt cttgactact tattcctagt tctgtattaa
      781 tatatctaag tattcctcca tattctttat caaggacatt caatactcga ttaatatatt
      841 cttcttttgc tgaactgagt gaaa
//
LOCUS       DAVLVP010000184          797 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.184, whole genome shotgun sequence.
ACCESSION   DAVLVP010000184 DAVLVP010000000
VERSION     DAVLVP010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.184"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..>797
                     /locus_tag="VYN69_002032"
     CDS             <1..>797
                     /locus_tag="VYN69_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M26"
                     /protein_id="HEV1534232.1"
                     /translation="YDRLTSPSWKYQSMVLPLLTLPEEKTVFMIANISTIGFGAYDRY
                     RSKVHPKGDNLNKFVEDNVREAAKRFRDHYDYWYKILEPENREKLYRSLLVYDAFKFG
                     RDNTEDKVTYQADFETDHPAIKYFFGPAGNNVVHNGHGAYATGDAFYYMAYRMLDKDG
                     AVTYTHEMTHNSDREIYLGGYGRRSGLGPEFYAKGLLQAPDHPYDPTITINSVLKYDD
                     SENSTRLQVADPTQRFNSAEDLHNYMHNMFDLIYTLEILEGRAVAKL"
BASE COUNT          298 a          107 c          151 g          241 t
ORIGIN      
        1 tatgatagat taactagtcc gtcatggaaa tatcaaagta tggtcttacc actattaact
       61 ttacctgaag aaaaaacagt atttatgata gcgaatattt caacaatagg atttggtgct
      121 tatgatagat atagaagtaa agtccatcct aaaggagata acttaaataa atttgtagaa
      181 gacaatgtaa gagaagctgc aaaaagattt agagatcatt atgattattg gtataaaata
      241 ttagagcctg agaatagaga gaaactttat agaagtttat tagtatacga tgcatttaaa
      301 tttggtcgag ataatactga agataaagta acttatcaag ctgattttga aactgatcat
      361 ccagctataa aatacttctt tggacctgcg gggaataatg ttgttcataa tggacacgga
      421 gcatatgcta ccggagatgc attctattat atggcatatc gtatgttgga taaagatgga
      481 gctgtaacat atactcatga aatgacacat aactctgata gagaaattta tcttggagga
      541 tatggaagaa gaagcggatt agggcctgag ttttatgcta agggcttatt acaagcacct
      601 gaccacccat atgatccaac aataacaatt aactctgtgt tgaaatatga tgattcagaa
      661 aattcaacaa gacttcaagt agctgatcca actcaacgat ttaatagtgc agaagaccta
      721 cataattata tgcataatat gtttgattta atatatactc ttgaaatatt agaaggtcgt
      781 gctgtagcta aattaga
//
LOCUS       DAVLVP010000185          762 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.185, whole genome shotgun sequence.
ACCESSION   DAVLVP010000185 DAVLVP010000000
VERSION     DAVLVP010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..762
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.185"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..667
                     /locus_tag="VYN69_002033"
     CDS             <1..667
                     /locus_tag="VYN69_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000677523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEV1534233.1"
                     /translation="TLIKTLSRLIKPLEGEVLLDNKSINSYKEKDLAKHIAILPQSPI
                     IPESITVADLVSRGRFPYRKPFKSLGKDDLEIINRSMVKANVEDLANNLVEELSGGQR
                     QRVWIALALAQDTSILLLDEPTTYLDISYQIELLDLLTDLNQKYKTTICMILHDINLT
                     ARYADYLFAIKEGKLVAEGKPEDILNDKLVKDIFNLEAKIIRDPISNSPLMIPIGKHH
                     INS"
BASE COUNT          286 a          137 c          120 g          219 t
ORIGIN      
        1 aacactcatt aaaaccttgt ctcgacttat aaagccatta gagggagaag tattgcttga
       61 taataaatca attaattctt ataaagaaaa agatttagca aaacacatag ctatattacc
      121 tcaatctcca ataatccctg aatcaataac agtagctgat cttgtaagcc gtggtcgttt
      181 cccctacaga aagcctttta agagtcttgg aaaagatgac cttgaaataa taaacagatc
      241 aatggttaag gccaatgttg aagatctagc aaataaccta gttgaagaac tttctggggg
      301 tcaaaggcaa agagtatgga tagctctagc cctagcccaa gatacaagta tcctactttt
      361 agatgagcca actacttact tggatatctc atatcaaata gaactattag acctcttgac
      421 tgatctaaac caaaaatata agacaaccat ttgcatgatt ttgcacgata taaatctaac
      481 agcaagatac gctgattacc tatttgcaat taaagaaggt aaacttgttg cagagggaaa
      541 gcctgaagat atactaaatg ataaactagt taaagatatc tttaatcttg aagcaaaaat
      601 tatacgtgac cctatttcca attcgcctct aatgattcct attggcaagc accatattaa
      661 ctcttaataa ttgaatattg gaattcattt aaaagtcgca aattttgaac tgaccctcaa
      721 aagttaggcc aaaaaactaa ctaaaggagg tcagtttttt aa
//
LOCUS       DAVLVP010000186          751 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.186, whole genome shotgun sequence.
ACCESSION   DAVLVP010000186 DAVLVP010000000
VERSION     DAVLVP010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.186"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..348
                     /locus_tag="VYN69_002034"
     CDS             <1..348
                     /locus_tag="VYN69_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000588926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEV1534234.1"
                     /translation="EEGLVDDIPRLRKYFKTKFRNRILDYIRKQESQKRRYDKEPYEE
                     VGEISHRISEGGLWLDDYYLFHETLRDYRNKQSKEKQEELERVLSNERFRGRQRVLRD
                     LRIVFKEFTIRTH"
     gene            442..513
                     /locus_tag="VYN69_002035"
     tRNA            442..513
                     /locus_tag="VYN69_002035"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:475..477,aa:Glu,seq:ttc)
BASE COUNT          295 a          112 c          168 g          176 t
ORIGIN      
        1 gaagaaggac tggtagacga tattccacgt ttaaggaaat atttcaaaac caagtttcga
       61 aatcgaattt tagactatat ccgtaagcag gaaagtcaga agcgtagata cgataaagaa
      121 ccctatgaag aagtgggaga gatcagtcat cgtataagtg agggaggtct ctggctagat
      181 gattattatc tctttcatga aacactaaga gattatagaa acaaacaaag taaagagaaa
      241 caagaagaac tagaacgcgt cttaagcaat gaacgatttc gagggcgtca aagagtatta
      301 agagacttac gcattgtgtt taaggagttt actatccgta cccactagta agtcatgcaa
      361 aaaaaatgaa aaaaattaga aaaagtagtt gacaaagttt gaaaagactg tataatagta
      421 agagttgaaa ataacaactc aggtccgttg gtcaaggggt taagacaccg ccttttcacg
      481 gcggtaacac gggttcgaat cccgtacgga ctatggtatg ttgcgtcagg agcacttgat
      541 gaaaaaaagt ttaaaaaagc ttaaaaatct tcaaaaaagt gttgacaagc gaaagcagtt
      601 gtgatatact aatatagttg tcgcttgaga gaagcaagtg acaaagacct ttgaaaactg
      661 aacaagacga accaatgtgc agggcgctac aacataagtt gtagtactga acaatgaaaa
      721 aacaataaat ctgtcagtga cagaaatgag t
//
LOCUS       DAVLVP010000187          707 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.187, whole genome shotgun sequence.
ACCESSION   DAVLVP010000187 DAVLVP010000000
VERSION     DAVLVP010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..707
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.187"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..629)
                     /locus_tag="VYN69_002036"
     CDS             complement(<1..629)
                     /locus_tag="VYN69_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000903082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloramphenicol acetyltransferase"
                     /protein_id="HEV1534235.1"
                     /translation="MLHPIFIIRRSWDGIFHLSEWKRNEEFDFFNKMDVPYLSMSSRI
                     EVTQAINFHKKHSISLYAIISWCVMSAINSIPELLMDTDGKIVWQYNQRGCSFTTLTS
                     EDKLNFSSFTMGDNLIEFVSAFNINKQKAEEGQKPNIDKNNIAYLSCVPWIDFLHVST
                     PMNLSKIDTVPRITWGKVIQENQRYFCTVNLQINHGMGDGLHVSNFFVL"
BASE COUNT          239 a          133 c           96 g          239 t
ORIGIN      
        1 aataacacaa aaaaattaga tacgtgtaat ccatctccca ttccatgatt aatttgtaaa
       61 ttcactgtac aaaaatatct ttggttctct tgaataactt ttccccacgt tattcgagga
      121 actgtatcaa tttttgaaag attcatagga gtgcttacat gtaaaaaatc aatccatgga
      181 acacaagaaa gataagcaat gttattcttg tcaatatttg gtttttgccc ttcttctgcc
      241 ttctgtttat ttatgttgaa cgcagaaaca aattcaatta aattatctcc catcgtaaat
      301 gaagaaaaat taagcttatc ttccgatgta agggtagtga aagaacaacc tctttgattg
      361 tattgccaga caatctttcc gtcggtatcc attagtaatt ctggaataga atttattgca
      421 ctcataacac accacgatat tatggcataa agtgatatcg aatgtttttt atgaaaattg
      481 atagcctgcg ttacttctat tcgtgaactc atagataaat aaggaacatc cattttattg
      541 aaaaagtcaa attcttcatt ccttttccac tctgataaat gaaaaattcc atcccaactc
      601 ctccttataa taaaaatagg gtgtagcatt cttttttcat gctacaccca caatcaacca
      661 tctttaaggc ttactctgac aagtaagtta ataagaatct ggactcc
//
LOCUS       DAVLVP010000188          676 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.188, whole genome shotgun sequence.
ACCESSION   DAVLVP010000188 DAVLVP010000000
VERSION     DAVLVP010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.188"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            60..>676
                     /locus_tag="VYN69_002037"
     CDS             60..>676
                     /locus_tag="VYN69_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018365310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEV1534236.1"
                     /translation="MDFEYFYNREAERFNFLKVPEILVDREEFRGLSAEAIILYSILL
                     KQTGMSFKNNWIDKEGRVFIYFTVEEIMKRRNISKPTAIKTLDELDVKKGIGLIERVR
                     LGLGKPNIIYVKDFMSIFQVKENDLQKSKNLTSEVKDFNLRSKENELQEVKNLDSNYI
                     ENNKSKYSKREYSFGENGLGTFQNVFLAAEDISDLQIIMNSQLEN"
BASE COUNT          269 a           82 c          131 g          194 t
ORIGIN      
        1 atagaagaaa aactatcgtc tttttctttg caaattttta agaagggagg tgatcttgca
       61 tggactttga atatttttat aacagagaag cggaaagatt taacttctta aaagtaccgg
      121 agatattagt tgatagagaa gaatttcggg gcttatcagc agaagcaatt atcctttatt
      181 ccatacttct taaacagaca ggaatgtcat ttaagaataa ctggatagac aaggaaggca
      241 gagtatttat ctattttact gtcgaagaaa ttatgaaaag aagaaatatc tcaaagccaa
      301 ctgccataaa aacattagat gagcttgatg taaaaaaagg aataggactg atcgaaagag
      361 taaggcttgg acttggtaag ccgaacatca tttatgttaa agactttatg agtatatttc
      421 aggtaaaaga aaatgactta cagaagtcaa aaaacttaac ttcagaagta aaagatttta
      481 acctcagaag taaagaaaat gaacttcaag aggttaagaa ccttgactct aactatatag
      541 agaataataa gagtaagtat agtaagagag aatatagttt tggtgaaaac ggacttggaa
      601 catttcaaaa tgtgttttta gctgctgaag atatatcgga tttacaaatc ataatgaact
      661 cacagcttga gaatta
//
LOCUS       DAVLVP010000189          638 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.189, whole genome shotgun sequence.
ACCESSION   DAVLVP010000189 DAVLVP010000000
VERSION     DAVLVP010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..638
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.189"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..418)
                     /locus_tag="VYN69_002038"
     CDS             complement(<1..418)
                     /locus_tag="VYN69_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEV1534237.1"
                     /translation="MGAARDFLELINHKGLPLFNPLVGDSHVNNRQEYDNTGSGNLQP
                     EKWNETAKQLYNVIMWLIILWNAKPDTPLFNFKDEVIKYKTYEPFESSIKRVNTTIKN
                     GSKGKTLTEMINGYRADNDIRDEICNFNILKNKIRDM"
BASE COUNT          186 a          131 c           72 g          249 t
ORIGIN      
        1 tcatatcacg aattttattt ttcagaatat taaagttaca aatttcatct ctaatatcgt
       61 tatccgctct gtagccatta atcatctcag tcagagtttt ccctttacta ccattcttta
      121 tagtagtatt tactcttttt atactgcttt caaaaggctc atatgtttta tacttaatta
      181 cttcgtcttt aaaattaaat aaaggtgtat ccggcttagc attccataaa ataatcaacc
      241 acattataac attataaagc tgctttgcag tttcattcca cttttcaggt tgtaaatttc
      301 cactccctgt attgtcatat tcttgtctat tatttacatg agaatcacct acaagcggat
      361 taaaaagagg tagccctttg tgattaatta gttctaaaaa atcccttgcc gcacccatgc
      421 aacactgaat aacttccttc ttgccatcaa ttttactctc agcgataaat acataatatg
      481 catcttcaat agttgctcct gcatcactat gtttttgttg atttgctaac agcttaatat
      541 gtgctttagg ctaaacttca aaatctctaa caattctttg tcttgtttca tgtcctctac
      601 aattcattta agttctcctt tacaagttta gtaactag
//
LOCUS       DAVLVP010000190          629 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.190, whole genome shotgun sequence.
ACCESSION   DAVLVP010000190 DAVLVP010000000
VERSION     DAVLVP010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 629)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 629)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..629
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.190"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..>629
                     /gene="phtB"
                     /locus_tag="VYN69_002039"
     CDS             <1..>629
                     /gene="phtB"
                     /locus_tag="VYN69_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal histidine triad protein PhtB"
                     /protein_id="HEV1534238.1"
                     /translation="LKDVSSDKVKLVDDILAFLAPIRHPERLGKPNAQITYTDDEIQV
                     AKLAGKYTTEDGYIFDPRDITSDEGDAYVTPHMTHSHWIKKDSLSEAERAAAQAYAKE
                     KGLTPPSTDHQDSGNTEAKGAEAIYNRVKAAKKVPLDRMPYNLQYTVEVKNGSLIIPH
                     YDHYHNIKFEWFDEGLYEAPKGYTLEDLLATVKYYVEHPNERPHSDNGF"
BASE COUNT          200 a          128 c          139 g          162 t
ORIGIN      
        1 actcaaggat gtctcaagtg ataaagtcaa gttagtggat gatattcttg ccttcttagc
       61 tccgattcgt catccagaac gtttaggaaa accaaatgcg caaattacct acactgatga
      121 tgagattcaa gtagccaagt tggcaggcaa gtacacaaca gaagacggtt atatctttga
      181 tcctcgtgat ataaccagtg atgaggggga tgcctatgta actccacata tgacccatag
      241 ccactggatt aaaaaagata gtttgtctga agctgagaga gcggcagccc aggcttatgc
      301 taaagagaaa ggtttgaccc ctccttcgac agaccatcag gattcaggaa atactgaggc
      361 aaaaggagca gaagctatct acaaccgcgt gaaagcagct aagaaggtgc cacttgatcg
      421 tatgccttac aatcttcaat atactgtaga agtcaaaaac ggtagtttaa tcatacctca
      481 ttatgaccat taccataaca tcaaatttga gtggtttgac gaaggtcttt atgaggcacc
      541 taaggggtat actcttgagg atcttttggc gactgtcaag tactatgtcg aacatccaaa
      601 cgaacgtccg cattcagata atggttttg
//
LOCUS       DAVLVP010000191          526 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.191, whole genome shotgun sequence.
ACCESSION   DAVLVP010000191 DAVLVP010000000
VERSION     DAVLVP010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.191"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..335
                     /locus_tag="VYN69_002040"
     CDS             <1..335
                     /locus_tag="VYN69_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008902124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PcfB family protein"
                     /protein_id="HEV1534239.1"
                     /translation="KGQLENISIDQIDLKDLKKELNKLGVSFSVMKNKESKNYEIFFQ
                     AKDIKVMEYAFKQVIAKENKKEKESILKQIKKYKDLSKNKDKTKEKGKRKVKEKVKPN
                     KKDMTREI"
     gene            462..>526
                     /locus_tag="VYN69_002041"
     CDS             462..>526
                     /locus_tag="VYN69_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEV1534240.1"
                     /translation="MATNKRYYWIKLKEEFFTDKR"
BASE COUNT          284 a           74 c           80 g           88 t
ORIGIN      
        1 agaaaggaca actagaaaat ataagcatag atcaaatcga cctcaaagac ttaaaaaaag
       61 aactaaacaa acttggagta agtttctcag taatgaaaaa caaagaaagc aaaaactatg
      121 aaatattctt ccaagccaaa gacataaaag taatggaata tgcctttaag caagtcatag
      181 ccaaggaaaa taaaaaagaa aaagaaagta tcctaaaaca aataaagaaa tacaaagacc
      241 tatccaaaaa caaagataag acaaaagaaa aaggaaaaag gaaagtaaaa gaaaaagtaa
      301 aaccaaacaa aaaagatatg accagagaaa tctaaaggga gtaacgaaaa gttacaccct
      361 taaataacca taataacaag acttccgaaa aacagaagtc cagaattccg aaaatcggaa
      421 atcaagaatt tcgattatcg aaaatcaaga aaggagcaga tatggcaaca aacaaaagat
      481 actattggat aaaattaaaa gaagaatttt tcacagacaa aaggat
//
LOCUS       DAVLVP010000192          494 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.192, whole genome shotgun sequence.
ACCESSION   DAVLVP010000192 DAVLVP010000000
VERSION     DAVLVP010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..494
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.192"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..>494
                     /locus_tag="VYN69_002042"
     CDS             <1..>494
                     /locus_tag="VYN69_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="pneumococcal-type histidine triad protein"
                     /protein_id="HEV1534241.1"
                     /translation="RTARGVAVPHGNHYHFIPYEQMSELEKRIARIIPLRYRSNHWVP
                     DSRPEQPSPQSTPEPSPSLQPAPNPQPAPSNPIDEKLVKEAVRKVGDGYVFEENGVSR
                     YIPAKDLSAETAAGIDSKLAKQESLSHKLGAKKTDLPSSDREFYNKAYDLLARIHQDL
                     LDNK"
BASE COUNT          162 a          114 c           95 g          123 t
ORIGIN      
        1 tcgaaccgcc agaggtgtag ctgtccctca tggtaaccat taccacttta tcccttatga
       61 acaaatgtct gaattggaaa aacgaattgc tcgtattatt ccccttcgtt atcgttcaaa
      121 ccattgggta ccagattcaa gaccagaaca accaagtcca caatcgactc cggaacctag
      181 tccaagtctg caacctgcac caaatcctca accagctcca agcaatccaa ttgatgagaa
      241 attggtcaaa gaagctgttc gaaaagtagg cgatggttat gtctttgagg agaatggagt
      301 ttctcgttat atcccagcca aggatctttc agcagaaaca gcagcaggca ttgatagcaa
      361 actggccaag caggaaagtt tatctcataa gctaggagct aagaaaactg acctcccatc
      421 tagtgatcga gaattttaca ataaggctta tgacttacta gcaagaattc accaagattt
      481 acttgataat aaag
//
LOCUS       DAVLVP010000193          443 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.193, whole genome shotgun sequence.
ACCESSION   DAVLVP010000193 DAVLVP010000000
VERSION     DAVLVP010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..443
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.193"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            complement(<1..137)
                     /locus_tag="VYN69_002043"
     CDS             complement(<1..137)
                     /locus_tag="VYN69_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEV1534242.1"
                     /translation="MNETKKYLVIKAIAQGKKTKKRACVELNLSERQINRLLLAYQQK
                     G"
     gene            308..379
                     /locus_tag="VYN69_002044"
     tRNA            308..379
                     /locus_tag="VYN69_002044"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:341..343,aa:Glu,seq:ttc)
BASE COUNT          140 a           73 c           82 g          148 t
ORIGIN      
        1 tttcctttct gttgataggc tagtagcaga cgattgattt gtctttcaga aagattgagt
       61 tcgacacagg cccgtttctt tgttttcttt ccttgggcta tagcttttat cacaagatat
      121 tttttcgttt cattcatatt cagttggatc cttttcatat gactattcta tcaaatggga
      181 cattttcacg ttcgatttac taaagacatt atcacattcg aatcacaatt aataaaaaaa
      241 attagaaaaa gtagttgaca aagtttgaaa agtctgtata atagtaagag ttgaaaataa
      301 caactcaggt ccgttggtca aggggttaag acaccgcctt ttcacggcgg taacacgggt
      361 tcgaatcccg tacggactat ggtatgttgc ggttggaaca cttgatgaaa aaaagtttaa
      421 aaaaagctta aaaatcttca aaa
//
LOCUS       DAVLVP010000194          417 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.194, whole genome shotgun sequence.
ACCESSION   DAVLVP010000194 DAVLVP010000000
VERSION     DAVLVP010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 417)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 417)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..417
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.194"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            <1..>417
                     /locus_tag="VYN69_002045"
     CDS             <1..>417
                     /locus_tag="VYN69_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEV1534243.1"
                     /translation="YWKLIQQDSRKLSDKRFYRPTFRMHLTNKEILDKILSYSEDLKH
                     HYQIYQLLLFHFQNKDPEKFFGLIEDNLKQVHPIFQTVFKTFLKNKEKIVNALQLPYS
                     NAKLEATNNLIKLIKRNAFGFRNFENFKKRIFIALN"
BASE COUNT          147 a           89 c           56 g          125 t
ORIGIN      
        1 ttactggaaa ctcatccaac aggatagtcg taaattgagc gataaacgtt tttatcgccc
       61 tacttttcgc atgcacttaa caaataaaga aattcttgac aagattttaa gctattcaga
      121 agacttgaaa caccactatc agatctatca actcttactt tttcactttc agaacaaaga
      181 ccctgagaaa tttttcggac tcattgagga caatctgaag caggttcatc ctatttttca
      241 gactgtcttt aaaacctttc taaagaacaa agaaaaaatc gtcaacgctc ttcaattacc
      301 ttattccaac gcaaaattgg aagcgaccaa taatctcatc aaacttatca aacgaaacgc
      361 ctttggtttt cggaactttg aaaacttcaa aaaaaggatt tttatcgctc tgaacat
//
LOCUS       DAVLVP010000195          364 bp    DNA     linear   BCT 22-JAN-2024
DEFINITION  TPA_asm: Streptococcus pneumoniae strain AUSMDU00072952
            SAMN33607483-rid17938963.denovo.195, whole genome shotgun sequence.
ACCESSION   DAVLVP010000195 DAVLVP010000000
VERSION     DAVLVP010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN33607483
            Sequence Read Archive: SRR23725588
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Streptococcus pneumoniae
  ORGANISM  Streptococcus pneumoniae
            Bacteria; Bacillota; Bacilli; Lactobacillales; Streptococcaceae;
            Streptococcus.
REFERENCE   1  (bases 1 to 364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (06-MAY-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 06-MAY-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001721465.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001721465.1
            Genome Coverage       :: 264x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/06/2023 22:36:00
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,046
            CDSs (total)                      :: 1,999
            Genes (coding)                    :: 1,880
            CDSs (with protein)               :: 1,880
            Genes (RNA)                       :: 47
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 42
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 119
            CDSs (without protein)            :: 119
            Pseudo Genes (ambiguous residues) :: 0 of 119
            Pseudo Genes (frameshifted)       :: 52 of 119
            Pseudo Genes (incomplete)         :: 54 of 119
            Pseudo Genes (internal stop)      :: 39 of 119
            Pseudo Genes (multiple problems)  :: 23 of 119
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..364
                     /organism="Streptococcus pneumoniae"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN33607483-rid17938963.denovo.195"
                     /strain="AUSMDU00072952"
                     /serotype="15B"
                     /host="Homo sapiens"
                     /db_xref="taxon:1313"
                     /country="Australia: Victoria"
                     /collection_date="2022"
                     /collected_by="MDU-PHL"
                     /note="genotype: ST2606"
     gene            1..364
                     /locus_tag="VYN69_002046"
                     /pseudo
     CDS             1..364
                     /locus_tag="VYN69_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800706.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="Rgg/GadR/MutR family transcriptional regulator"
BASE COUNT          137 a           39 c           73 g          115 t
ORIGIN      
        1 aaggattgga ggtaataaaa aatgagcaac aaaaactttt tgagaagagt ggggaaaaaa
       61 ctcataaaat aaatacaatt atggttcagg gattatgaaa tcagctagat tgtaatcatg
      121 tagttagtag agatgatcta aatttagtat atgactattt attccagaag gaacgatggg
      181 agtcctatga gataacgctg attggtaatt tatatcatct ttttgaaaca gattatattt
      241 acatggtcgg gaaagaaata ctagagcgta cacattatta cgaaaaaatc ggcaagaata
      301 gaaatttagt tgtgtcagct tgtttaaatt tttggttctg ttgccttgaa aattcacatc
      361 taat
//
